Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Hao Xing
Date created: 2013-05-11 11:00:00
Date modified: 2015-05-08 01:14:08
Hydrocarbon Sensor
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_M41006_sequence (Version 1) |
Description
This is a sequence for hydrocarbon sensor. The input for this sensor is n-alkane chain and output is LacI. It has a sequence which encodes membrane protein (AlkL) and chemotaxis protein (AlkN) responsible for hydrocarbon sensing. This sequence does not code for any major alkane degrading enzymes except AlkL and AlkN.Notes
This sensor is specific for our system. The output can be changed by removing LacI gene and adding other gene.Source
Canosa, I., et al. "A Positive Feedback Mechanism Controls Expression of AlkS, the Transcriptional Regulator of the Pseudomonas Oleovorans Alkane Degradation Pathway." Molecular microbiology 35.4 (2000): 791-9.Van Beilen, JB, et al. "Analysis of Pseudomonas Putida Alkane-Degradation Gene Clusters and Flanking Insertion Sequences: Evolution and Regulation of the Alk Genes." Microbiology 147.6 (2001): 1621-30.
| Access | Instance | Definition |
| public public public public public public public public public public public public public public | BBa_J23119 BBa_B0034 BBa_M41000 BBa_M41001 BBa_B0015 BBa_M41002 BBa_M41003 BBa_B0034 BBa_M41004 BBa_B0015 BBa_M41005 BBa_B0034 BBa_C0012 BBa_B0015 | BBa_J23119 BBa_B0034 BBa_M41000 BBa_M41001 BBa_B0015 BBa_M41002 BBa_M41003 BBa_B0034 BBa_M41004 BBa_B0015 BBa_M41005 BBa_B0034 lacI BBa_B0015 |