BBa_K1430000

BBa_K1430000 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1430000

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Cedric Bl??tz

Date created: 2014-09-29 11:00:00

Date modified: 2015-05-08 01:10:23

yeast 2-micron

Details

• Types: DnaRegion
• Roles: Regulatory; promoter
• Sequences: BBa_K1430000_sequence (Version 1)

Description

Mutated 2-micron for plasmid amplification in yeast cells. The 2 micron sequence was mutated that internal restriction sites (for Pre/-Suffix enzymes) are lost.

Notes

A silent mutation was created via PCR to delete restriction site for Xba1.

Source

pDEST-GAD (plasmid, Invitrogen)

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
2-micron ori	1,1343	sequence_feature, feature/misc

Other Properties

• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1430000/1

Member of these Collections

• iGEM Parts Registry