Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Maryam Ige, Janki Patel
Date created: 2014-06-22 11:00:00
Date modified: 2015-05-08 01:10:13
GST-melittin
| Types | DnaRegion |
| Roles | Coding CDS |
| Sequences | BBa_K1382001_sequence (Version 1) |
Description
This consists of melittin fused to GST (Glutathione S-Tranferase) with a TEV cleavage site. Melittin is a toxin which causes cell death. GST-melittin is an inactive form of melittin, which ensures that melittin can be produced in a cell without killing the host cell. To activate melittin, TEV protease is added to cleave melittin from GST.Notes
Thrombin was the initial cleavage site, however we decided to use TEV because it was more efficient. As a result, we have both thrombin and TEV cleavage sites.Source
Melittin- Apis Mellifera| Sequence Annotation | Location | Component / Role(s) |
| Thrombin cleavage site GST TEV cleavage site Melittin | 661,678 1,654 685,705 709,792 | feature/misc sequence_feature tag feature/tag feature/misc sequence_feature CDS feature/cds |