Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Kelly Cochran, Mike Quaney
Date created: 2014-07-21 11:00:00
Date modified: 2015-08-31 04:08:28
Ammeline Fitness Module with Riboswitch N2 + TetA (T138C, C160A)
| Types | DnaRegion |
| Roles | engineered_region Measurement |
| Sequences | BBa_J119365_sequence (Version 1) |
Description
The construct is a fitness module for ammeline. The P5 promoter drives a mutated riboswitch (N2 with mutations T138C, C160A) that allows translation in the presence of ammeline (made by demethylation of melamine) of a TetA fitness gene.Notes
N2, TetASource
N2, TetA| Sequence Annotation | Location | Component / Role(s) |
| P5 promoter Ammeline Riboswitch TetA T to C mutation C to A mutation | 19,55 73,190 185,1382 138,138 160,160 | feature/promoter promoter feature/rbs ribosome_entry_site CDS feature/cds feature/mutation sequence_alteration feature/mutation sequence_alteration |