Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: MWSU Genetics Lab Fall 2012 Tues 8:00
Date created: 2013-01-07 12:00:00
Date modified: 2015-08-31 04:08:27
pTac with mutations
| Types | DnaRegion |
| Roles | Regulatory promoter |
| Sequences | BBa_J119129_sequence (Version 1) |
Description
This part was designed to see the effects of mutations on a functional promoter. Golden Gate Assembly was used to insert the mutations into Part J100091 at the double terminator sites. A mutation was created by removing the ATTA sequence after the -35 location. This had the effect of reducing the spacing between the -35 and -10 elements from 16 base pairs to 12 base pairs.Notes
The oligonucleotides needed to have sticky ends compatible with those produced by BsaI digestion of J100091 so that Golden Gate Assembly could be used.Source
Oligonucleotides.| Sequence Annotation | Location | Component / Role(s) |
| -35 region -10 region | 11,16 29,34 | sequence_feature feature/dna sequence_feature feature/dna |