Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Hannah Itell
Date created: 2014-07-24 11:00:00
Date modified: 2015-08-31 04:08:23
Strong Promoter + M6-R1 Ammeline Riboswitch + RFP
| Types | DnaRegion |
| Roles | DNA sequence_feature |
| Sequences | BBa_J100174_sequence (Version 1) |
Description
This has a seven mutations from the original M6 riboswitch sequence found in part J119332. Five of these mutations are part of the intentional design of the riboswitch, in order for it to be more effective than the original M6 riboswitch. As with part J119332, base 432 is mutated from an A (tyrosine) to a G (cysteine) in the RFP CDS. The two additional mutations are T20C in the promoter region and C852T in the RFP CDS.Notes
N/ASource
J119332| Sequence Annotation | Location | Component / Role(s) |
| P5 M6-R1 Ammeline Riboswitch RFP A to G Change T to C Change C to T Change | 18,55 73,187 185,869 432,432 20,20 852,852 | feature/promoter promoter feature/misc sequence_feature feature/cds CDS sequence_alteration feature/mutation feature/mutation sequence_alteration feature/mutation sequence_alteration |