BBa_K654049
BBa_K654049 Version 1 (Component)
Promoter/RBS Tester psbA2 Reference Dual Luciferase Assay

BBa_K654050
BBa_K654050 Version 1 (Component)
Promoter Control psbA2 Reference Dual Luciferase Assay

BBa_K654054
BBa_K654054 Version 1 (Component)
Promoter Tester 6803 Consensus RBS psbA2 Reference Dual Luciferase Assay

BBa_K654051
BBa_K654051 Version 1 (Component)
Promoter Tester 6803 Native RBS sigA Reference Dual Luciferase Assay

BBa_K654052
BBa_K654052 Version 1 (Component)
Promoter/RBS Tester sigA Reference Dual Luciferase Assay

BBa_K654053
BBa_K654053 Version 1 (Component)
Promoter Control sigA Reference Dual Luciferase Assay

BBa_K654055
BBa_K654055 Version 1 (Component)
Promoter Tester 6803 Consensus RBS sigA Reference Dual Luciferase Assay

BBa_K654096
BBa_K654096 Version 1 (Component)
E. coli Reference Promoter (fusion of Anderson 0.51 and 1.00 promoters)

BBa_K654097
BBa_K654097 Version 1 (Component)
Anderson 0.10 E. coli Reference Promoter

BBa_K654098
BBa_K654098 Version 1 (Component)
Anderson 0.01 E. coli Reference Promoter

BBa_K750001
BBa_K750001 Version 1 (Component)
LuxI expression device activated by arabinose(Regulated by RBS of 1.0 strength)

BBa_K750002
BBa_K750002 Version 1 (Component)
LuxI expression device activated by arabinose(Regulated by RBS of 0.6 strength)

BBa_K750003
BBa_K750003 Version 1 (Component)
LuxI expression device activated by arabinose(Regulated by RBS of 0.3 strength)

BBa_K750004
BBa_K750004 Version 1 (Component)
LuxI expression device activated by arabinose(Regulated by RBS of 0.07 strength)

BBa_K750005
BBa_K750005 Version 1 (Component)
LuxI expression device activated by arabinose(Regulated by RBS of 0.01 strength)

SBOLDesigner CAD Tool
SBOLDesigner Version 3.1 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are the ability to add variant collections to combinatorial derivations, enumerating those collections, and the ability to view sequence features hierarchically. There are also some small changes to the way that preferences work in regards to saving a design with incomplete sequences.

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

BBa_J24814
BBa_J24814 Version 1 (Component)
RBS + Renilla

BBa_J24815
BBa_J24815 Version 1 (Component)
J23101 promoter with Renilla gene attached

BBa_K127003
BBa_K127003 Version 1 (Component)
Polystyrene binding peptide inserted CPX generated by qurum sensing and constitutive GFP genarator

BBa_J24820
BBa_J24820 Version 1 (Component)
Renilla luciferase reporter device for DLARAP

BBa_M11079
BBa_M11079 Version 1 (Component)
Copper binding paired with Polystyrene binding

BBa_K216014
BBa_K216014 Version 1 (Component)
PyeaR with Renilla luciferase

BBa_I1021
BBa_I1021 Version 1 (Component)
Antisense RNA w/BBa_I1020 Interference (KISS)

BBa_I1022
BBa_I1022 Version 1 (Component)
Antisense RNA w/BBa_I1020 Interference (micRNA)

BBa_J95031
BBa_J95031 Version 1 (Component)
BioBrick promoter and RBS measurement reference standard for R. sphaeroides