gpS
BBa_K836004 Version 1 (Component)
Lysis inhibitor from Enterobacteria phage lambda (codon usage optimized for R. opacus)

BBa_K836005
BBa_K836005 Version 1 (Component)
Lysozyme from Rhodococcus phage RER2 (codon usage optimized for R, opacus)

gpS
BBa_K836006 Version 1 (Component)
Lysis protein S from Enterobacteria phage lambda (codon usage optimized for R. opacus)

nitR
BBa_K836007 Version 1 (Component)
Nitrilase regulator from R. rhodochrous (codon usage optimized for R. opacus)

SEGA
SEGA_collection Version 1 (Collection)
In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.

BBa_K124017
BBa_K124017 Version 1 (Component)
Bacteriophage Lysis Cassette S105, R, and Rz

SV40 NLS
BBa_J63008 Version 1 (Component)
SV40 nuclear localization sequence from SV40; yeast codon optimized

BBa_K112122
BBa_K112122 Version 1 (Component)
Cre with stop codon

BBa_C0361
BBa_C0361 Version 1 (Component)
luxI (+LVA) - Codon-optimised for E.coli

BBa_C0378
BBa_C0378 Version 1 (Component)
lasI (+LVA) - Codon-optimised for E.coli

BBa_C0362
BBa_C0362 Version 1 (Component)
luxR - codon-optimised for E.coli

BBa_J69010
BBa_J69010 Version 1 (Component)
3OC6-HSL Lux Reciever Device - Codon-Optimised

BBa_J69011
BBa_J69011 Version 1 (Component)
3OC6-HSL Lux Reciever Device (Codon-Optimised) with GFP Reporter

BBa_Z0301
BBa_Z0301 Version 1 (Component)
Venus YFP - E. coli codon optimized

mCherry
BBa_J06505 Version 1 (Component)
monomeric RFP optimized for bacteria

VR2
BBa_J18907 Version 1 (Component)
VR2: Reverse Primer to pSB1 Biobrick Vectors (optimized VR)

BBa_J70029
BBa_J70029 Version 1 (Component)
RBS + mCherry gene, optimized for expression in Me. florum and E. coli

BBa_K199007
BBa_K199007 Version 1 (Component)
CCAUC Suppressor tRNA (9-bp Anticodon and Produces Serine)

BBa_K199008
BBa_K199008 Version 1 (Component)
CCAUC Suppressor tRNA (10-bp Anticodon and Produces Serine)

BBa_C100014
BBa_C100014 Version 1 (Component)
LMS ORF (B0034 optimized)

BBa_C100015
BBa_C100015 Version 1 (Component)
LIS ORF (B0034 optimized)

BBa_C100016
BBa_C100016 Version 1 (Component)
Oe23 ORF (B0034 optimized)

BBa_C100017
BBa_C100017 Version 1 (Component)
Myrcene Synthase (B0034 optimized)

BBa_P100114
BBa_P100114 Version 1 (Component)
LMS ORF with RBS (optimized)

BBa_P100115
BBa_P100115 Version 1 (Component)
LIS ORF with RBS (optimized)

BBa_P100116
BBa_P100116 Version 1 (Component)
Oe23 ORF with RBS (optimized)

BBa_P100117
BBa_P100117 Version 1 (Component)
Myrcene Synthase ORF with RBS (optimized)

BBa_P101114
BBa_P101114 Version 1 (Component)
LMS ORF with RBS and Term (optimized)

BBa_P101115
BBa_P101115 Version 1 (Component)
LIS ORF with RBS and Term (optimized)

BBa_P101116
BBa_P101116 Version 1 (Component)
Oe23 ORF with RBS and Term (optimized)

BBa_P101117
BBa_P101117 Version 1 (Component)
Myrcene Synthase ORF with RBS and Term (optimized)

BBa_P111114
BBa_P111114 Version 1 (Component)
LMS complete construct (R0040, B0034 optimized)

BBa_P121114
BBa_P121114 Version 1 (Component)
LMS complete construct (F2620, B0034 optimized)

BBa_P111115
BBa_P111115 Version 1 (Component)
LIS complete construct (R0040, B0034 optimized)

BBa_P121115
BBa_P121115 Version 1 (Component)
LIS complete construct (F2620, B0034 optimized)

BBa_P111116
BBa_P111116 Version 1 (Component)
Oe23 complete construct (R0040, B0034 optimized)

BBa_P121116
BBa_P121116 Version 1 (Component)
Oe23 complete construct (F2620, B0034 optimized)

BBa_P111117
BBa_P111117 Version 1 (Component)
Myrcene Synthase complete construct (R0040, B0034 optimized)

BBa_P121117
BBa_P121117 Version 1 (Component)
Myrcene Synthase complete construct (F2620, B0034 optimized)

BBa_K199031
BBa_K199031 Version 1 (Component)
pBad-CCAUC Suppressor tRNA (9-bp Anticodon)

BBa_K199032
BBa_K199032 Version 1 (Component)
pBad-CCAUC Suppressor tRNA (10-bp Anticodon)

BBa_K199071
BBa_K199071 Version 1 (Component)
I13453:K199014: Pbad promomtor with the suppressor tRNA of the codon AGGAC

BBa_K194001
BBa_K194001 Version 1 (Component)
GFP (a yeast- and FACS-optimized GFP variant),

BBa_K234092
BBa_K234092 Version 1 (Component)
Stop codon + CYC1 Terminator

BioScaffol
BBa_K259002 Version 1 (Component)
BioScaffold Linker - Removes Stop Codons & scars & replaces with a Gly-Ser linker

BioScaffol
BBa_K259004 Version 1 (Component)
BioScaffold Linker - Removes Stop Codons & scars & replaces with a Gly-Ser linker

BBa_K199056
BBa_K199056 Version 1 (Component)
CCAUC tRNA (9-bp Anticodon) with RFP Reporter

BBa_K199057
BBa_K199057 Version 1 (Component)
CCAUC tRNA (10-bp Anticodon) with RFP Reporter

BBa_E2030
BBa_E2030 Version 1 (Component)
enhanced version of EYFP, yeast-optimized

BBa_K216004
BBa_K216004 Version 1 (Component)
Trz hybrid signal transduction protein