Counter
BBa_J05050 Version 1 (Component)
Counter

BBa_K329017
BBa_K329017 Version 1 (Component)
Population counter Tetracycline resistant - PmS-MTet-LuxI

BBa_K329019
BBa_K329019 Version 1 (Component)
Population counter Tetracycline resistant - Final PmS-MTet

BBa_K329023
BBa_K329023 Version 1 (Component)
Population counter expressing RFP - PsS-RFP

BBa_K329022
BBa_K329022 Version 1 (Component)
Population counter expressing RFP - PmS-RFP

BBa_K329068
BBa_K329068 Version 1 (Component)
Population counter Tetracycline resistant - PsS-WTet-LuxI

BBa_K329067
BBa_K329067 Version 1 (Component)
Population counter expressing RFP - PsM-RFP

BBa_K329066
BBa_K329066 Version 1 (Component)
Population counter expressing RFP - PsW-RFP

BBa_K225002
BBa_K225002 Version 1 (Component)
RTC counter element 1

BBa_K225003
BBa_K225003 Version 1 (Component)
RTC counter element 2

BBa_K917010
BBa_K917010 Version 1 (Component)
Kanamycin resistance+lac promoter+RFP+sacB selection-counterselection cassette

BBa_M1618
BBa_M1618 Version 1 (Component)
Cell Replication Counter Device

BBa_K1461227
BBa_K1461227 Version 1 (Component)
resettable single counter system using ecf20

BBa_K1461229
BBa_K1461229 Version 1 (Component)
resettable single counter system using ecf11

BBa_K1461232
BBa_K1461232 Version 1 (Component)
single counter

BBa_K1461235
BBa_K1461235 Version 1 (Component)
resettable counter device using ecf20 (w/o crRBS, taRNA)

BBa_K1461236
BBa_K1461236 Version 1 (Component)
resettable counter device using ecf11 (w/o crRBS, taRNA)

BBa_K1461239
BBa_K1461239 Version 1 (Component)
σ-Re Counter (double, using ecf20)

BBa_K1461240
BBa_K1461240 Version 1 (Component)
σ-Re Counter (double, using ecf11)

BBa_K1461241
BBa_K1461241 Version 1 (Component)
σ-Re Counter (triple)

BBa_K1351023
BBa_K1351023 Version 1 (Component)
upp cassette as counter-selection marker

BBa_K1945002
BBa_K1945002 Version 1 (Component)
Homologous Recombination Intermediate for Counter-selection in Synechocystis sp. PCC 6803

BBa_K329069
BBa_K329069 Version 1 (Component)
Population counter Tetracycline resistant after recombination - PmS-MTet-LuxI

SEGA
SEGA_collection Version 1 (Collection)
In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.