BBa_K1461236
BBa_K1461236 Version 1 (Component)
resettable counter device using ecf11 (w/o crRBS, taRNA)

BBa_K1461239
BBa_K1461239 Version 1 (Component)
σ-Re Counter (double, using ecf20)

BBa_K1461240
BBa_K1461240 Version 1 (Component)
σ-Re Counter (double, using ecf11)

BBa_K1461241
BBa_K1461241 Version 1 (Component)
σ-Re Counter (triple)

BBa_K1351023
BBa_K1351023 Version 1 (Component)
upp cassette as counter-selection marker

BBa_K1465225
BBa_K1465225 Version 1 (Component)
Knock-down of phosphofructokinase A using sRNA (sRNA:pfkA)

BBa_K1895006
BBa_K1895006 Version 1 (Component)
Alternative electrically induced expression system using the dnaK promoter.

BBa_K2163000
BBa_K2163000 Version 1 (Component)
Measurement of Protease Activity using FRET

BY-Toggle
BBa_K1908000 Version 1 (Component)
Blue-Yellow fluorescent protein toggle switch using pLac and OmpC promoter.

BBa_K1945002
BBa_K1945002 Version 1 (Component)
Homologous Recombination Intermediate for Counter-selection in Synechocystis sp. PCC 6803

BBa_M50053
BBa_M50053 Version 1 (Component)
FRET-based glucose sensor using a glucose binding protein, mRuby3 and cometGFP

SBOLDesigner CAD Tool
SBOLDesigner Version 3.1 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are the ability to add variant collections to combinatorial derivations, enumerating those collections, and the ability to view sequence features hierarchically. There are also some small changes to the way that preferences work in regards to saving a design with incomplete sequences.

BBa_K322924
BBa_K322924 Version 1 (Component)
Alternate targeting contruct for TnaA replacement using BRIDGE

BBa_K329069
BBa_K329069 Version 1 (Component)
Population counter Tetracycline resistant after recombination - PmS-MTet-LuxI

BBa_J70652
BBa_J70652 Version 1 (Component)
This tests adding an internal strep tag linker to a protein using J70590 as a te

BBa_J70653
BBa_J70653 Version 1 (Component)
This tests adding an internal strep tag linker to a protein using J70590 as a te

BBa_K656006
BBa_K656006 Version 1 (Component)
IPTG Reporter Using Cohesin-Gly-Ser-Gly-Cohesin

BBa_K737000
BBa_K737000 Version 1 (Component)
We got this part from the mutant of E.coli strain K12, DH5α,using PCR with the primers we desig

BBa_K737001
BBa_K737001 Version 1 (Component)
We got this part from the mutant of E.coli strain K12, DH5α,using PCR with the primers we desig

SEGA
SEGA_collection Version 1 (Collection)
In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.

SBOLDesigner CAD Tool
SBOLDesigner Version 3.1 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are the ability to add variant collections to combinatorial derivations, enumerating those collections, and the ability to view sequence features hierarchically. There are also some small changes to the way that preferences work in regards to saving a design with incomplete sequences.

BBa_J70064
BBa_J70064 Version 1 (Component)
Adds 6 his suffix, using BioScaffold part J70010 (PpiI)

BBa_J70065
BBa_J70065 Version 1 (Component)
Adds 6 his suffix, using BioScaffold part J70009 (PpiI)

BBa_J70082
BBa_J70082 Version 1 (Component)
Adds 6 his prefix, using BioScaffold part J70030 (PpiI)

BBa_J70084
BBa_J70084 Version 1 (Component)
Adds 6 his suffix, using BioScaffold part J70030 (PpiI) in pSB1AT3

BBa_J70074
BBa_J70074 Version 1 (Component)
Adds 6 his suffix using BioScaffold part J70012

BBa_J70072
BBa_J70072 Version 1 (Component)
Adds 6 his prefix using BioScaffold part J70012

BBa_J70062
BBa_J70062 Version 1 (Component)
Adds 6 his prefix using BioScaffold part J70010

BBa_J69591
BBa_J69591 Version 1 (Component)
GFP reporter device - using Standard Promoter (1RPU)

BBa_K233001
BBa_K233001 Version 1 (Component)
Snowdrift 1 , using TorA. A module to secrete B-galactosidase using the TAT-export Pathway

Co-op
BBa_K233002 Version 1 (Component)
Snowdrift 2 , using YcdB. A module to secrete B-galactosidase using the TAT-export Pathway

BBa_K233312
BBa_K233312 Version 1 (Component)
GFP secretion device using Ycdb

BBa_K325801
BBa_K325801 Version 1 (Component)
Theophylline activated quiescence inducer (using Rcd)

SBOLDesigner CAD Tool
SBOLDesigner Version 3.0 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.