BBa_M36989
BBa_M36989 Version 1 (Component)
Secreting Antifreeze Protein Correcting Forward Primer

BBa_M36988
BBa_M36988 Version 1 (Component)
Secreting Antifreeze Protein Correcting Reverse Primer

P<sub>rsaA
BBa_K531000 Version 1 (Component)
P_rsaA: A constitutive promoter and RBS from <i>Caulobacter crescentus</i>

BBa_K531001
BBa_K531001 Version 1 (Component)
<i>C. crescentus</i> S-layer RsaA C-term

P<sub>xyl<
BBa_K531002 Version 1 (Component)
P_xyl: Inducible promoter and RBS from <i>Caulobacter crescentus</i>

BBa_K531004
BBa_K531004 Version 1 (Component)
dspB from Actinobacillus actinomycetemcomitansis optimized for Caulobacter crescentus

BBa_K531005
BBa_K531005 Version 1 (Component)
P_xyl promoter+optimized <i>esp</i>+<i>rsaA</i> secretion tag

BBa_K531006
BBa_K531006 Version 1 (Component)
Optimized esp gene for <i>C. crescentus</i>

BBa_K553020
BBa_K553020 Version 1 (Component)
Mammaliam Secreted Beta Lactamase

BBa_K554002
BBa_K554002 Version 1 (Component)
HlyA secretion signal peptide

SBOLDesigner CAD Tool
SBOLDesigner Version 3.1 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are the ability to add variant collections to combinatorial derivations, enumerating those collections, and the ability to view sequence features hierarchically. There are also some small changes to the way that preferences work in regards to saving a design with incomplete sequences.

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

mRFP1
BBa_I13502 Version 1 (Component)
Screening plasmid intermediate

BBa_K125810
BBa_K125810 Version 1 (Component)
slr2016 signal sequence + GFP fusion for secretion of GFP

BBa_K112328
BBa_K112328 Version 1 (Component)
{rbs_ompT>} {< Cre>}

BBa_K112340
BBa_K112340 Version 1 (Component)
{rbs_ompT>} {< Cre>}{< phoA!}{< b1006>}

BBa_K112348
BBa_K112348 Version 1 (Component)
{rbs_ompT>} {< Cre>}{< S-Tag!}{< b1006>}

BBa_K112360
BBa_K112360 Version 1 (Component)
{Pbad}{rbs_ompT>} {< Cre>}{< phoA!}{< b1006>}

BBa_K112368
BBa_K112368 Version 1 (Component)
{Pbad}{rbs_ompT>} {< Cre>}{< S-Tag!}{< b1006>}

BBa_K112122
BBa_K112122 Version 1 (Component)
Cre with stop codon

BBa_J70033
BBa_J70033 Version 1 (Component)
A BioMortar Part for Creating GSGS Protein Fusions (see Design page)

BBa_I13501
BBa_I13501 Version 1 (Component)
Screening plasmid intermediate

BBa_M10092
BBa_M10092 Version 1 (Component)
{Pbad.rbs.prepro&#707;} For arabinose induced secretion

BBa_K233001
BBa_K233001 Version 1 (Component)
Snowdrift 1 , using TorA. A module to secrete B-galactosidase using the TAT-export Pathway

Co-op
BBa_K233002 Version 1 (Component)
Snowdrift 2 , using YcdB. A module to secrete B-galactosidase using the TAT-export Pathway

HfsG
BBa_K196002 Version 1 (Component)
HfsG protein from <i>Caulobacter crescentus</i>

HfsH
BBa_K196003 Version 1 (Component)
HfsH protein from <i>Caulobacter crescentus</i>

BBa_K196004
BBa_K196004 Version 1 (Component)
HfsG + HfsH proteins from <i>Caulobacter crescentus</i>

BBa_K196005
BBa_K196005 Version 1 (Component)
HfsG + HfsH proteins from <i>Caulobacter crescentus</i> + ter (BBa_B0015)

BBa_K228867
BBa_K228867 Version 1 (Component)
pSB1A2-lacZ screening plasmid

BBa_K233311
BBa_K233311 Version 1 (Component)
Protein(GFP) secretion device

BBa_K233312
BBa_K233312 Version 1 (Component)
GFP secretion device using Ycdb

BBa_K284031
BBa_K284031 Version 1 (Component)
Cre Recombinase without start codon

BBa_K284043
BBa_K284043 Version 1 (Component)
AGTC repetition and CRE recombinase under constitutive promoter

BBa_K215012
BBa_K215012 Version 1 (Component)
Secretion Signal and Streptavidin Binding Tags on GFP

BBa_K206012
BBa_K206012 Version 1 (Component)
Cre Recombinase with LVA Tag

BBa_K284029
BBa_K284029 Version 1 (Component)
Cre recombinase under the control of QseA promotor

BBa_K212004
BBa_K212004 Version 1 (Component)
secretion signal peptide + GFPmut3b

BBa_K284150
BBa_K284150 Version 1 (Component)
Alpha Hemolysin Secretion System

BBa_K318010
BBa_K318010 Version 1 (Component)
T + araC + pBad + RBS + Cre + T

BBa_K318020
BBa_K318020 Version 1 (Component)
T + araC + pBad + RBS + Cre

BBa_K315032
BBa_K315032 Version 1 (Component)
pBad + RBS + cre + pTet + loxP forward + RBS + RFP + lox5171 forward

BBa_K315033
BBa_K315033 Version 1 (Component)
pBad + RBS + cre + pTet + lox5171 forward + RBS + RFP + lox5171 forward

BBa_K315034
BBa_K315034 Version 1 (Component)
pBad + RBS + cre + pTet + lox5171 forward + RBS + RFP + lox5171 reverse

BBa_K315036
BBa_K315036 Version 1 (Component)
pBad + RBS + cre + pTet + lox5171 forward + RBS + RFP + loxm2 forward

BBa_K315037
BBa_K315037 Version 1 (Component)
pBad + RBS + cre + pTet + lox5171 forward + RBS + RFP + loxN forward

BBa_K315038
BBa_K315038 Version 1 (Component)
pBad + RBS + cre + pTet + lox5171 forward + RBS + RFP + lox2272 forward

BBa_K315039
BBa_K315039 Version 1 (Component)
pBad + RBS + cre + pTet + lox5171 forward + RBS + RFP + loxBri forward

BBa_K390003
BBa_K390003 Version 1 (Component)
pilA secretion tag for Synechocystis sp. PCC 6803

BBa_K390004
BBa_K390004 Version 1 (Component)
art+ secretion tag for Synechocystis sp. PCC 6803