//terminator/double
type: Collection
persistent identity: terminator double
display id: terminator_double
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
owned by: james
owned by: myers
member: Zwf-Device
member: BBa_K1031224
member: BBa_K1031422
member: BBa_K1031620
member: BBa_C0450
member: BBa_K1031100
member: BBa_K1031410
member: BBa_K1486025
member: BBa_K1031625
member: BBa_K1031221
member: BBa_K2122100
member: BBa_K1031622
member: BBa_K1031923
member: BBa_K1031114
member: BBa_K1031510
member: BBa_B0014
member: BBa_K1031941
member: BBa_K1031804
member: BBa_K2122000
member: Reporter +
member: BBa_K1031623
member: BBa_K933000
member: BBa_K1031111
member: BBa_K1031624
member: BBa_K1031520
member: BBa_K1031913
member: BBa_B0025
member: BBa_K1031112
member: BBa_K1031302
member: BBa_K1031521
member: BBa_K1486005
member: BBa_B0017
member: BBa_K1031931
member: BBa_K1031621
member: BBa_B0024
member: BBa_K1031113
member: BBa_K1031311
member: BBa_K1031922
member: BBa_K1031803
member: BBa_B0015
member: BBa_K259006
member: BBa_I11013
member: BBa_K1031222
member: BBa_K1031420
member: BBa_K1031916
member: BBa_K1031223
member: BBa_K1031421
member: BBa_K1486001
member: BBa_K1031301
member: BBa_K1031917
member: BBa_K1486009
member: BBa_K1031710

BBa_K1031917
type: ComponentDefinition
persistent identity: BBa K1031917
display id: BBa_K1031917
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: J23117-XylS-Terminator
creator: He Shuaixin
created: 2013-10-13T11:00:00Z
modified: 2015-05-08T01:08:48Z
discontinued: false
dominant: false
group u list: _1338_
m user id: 0
owner id: 15843
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: 117-XylS-TT
mutable notes: molecular cloning
mutable provenance: Pseudomonas.putida
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: J23117
sequence annotation: XylS
sequence annotation: B0015
sequence: https://synbiohub.org/public/igem/BBa_K1031917_sequence/1

J23117
type: SequenceAnnotation
persistent identity: annotation2369600
display id: annotation2369600
version: 1
top level: BBa_K1031917
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1031917/annotation2369600/range2369600/1

https://synbiohub.org/public/igem/BBa_K1031917/annotation2369600/range2369600/1
type: Range
persistent identity: range2369600
display id: range2369600
version: 1
direction: direction/forward
top level: BBa_K1031917
start: 1
end: 35
orientation: inline

XylS
type: SequenceAnnotation
persistent identity: annotation2369601
display id: annotation2369601
version: 1
top level: BBa_K1031917
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1031917/annotation2369601/range2369601/1

https://synbiohub.org/public/igem/BBa_K1031917/annotation2369601/range2369601/1
type: Range
persistent identity: range2369601
display id: range2369601
version: 1
direction: direction/forward
top level: BBa_K1031917
start: 63
end: 1028
orientation: inline

B0015
type: SequenceAnnotation
persistent identity: annotation2369602
display id: annotation2369602
version: 1
top level: BBa_K1031917
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1031917/annotation2369602/range2369602/1

https://synbiohub.org/public/igem/BBa_K1031917/annotation2369602/range2369602/1
type: Range
persistent identity: range2369602
display id: range2369602
version: 1
direction: direction/forward
top level: BBa_K1031917
start: 1035
end: 1163
orientation: inline

BBa_K933000
type: ComponentDefinition
persistent identity: BBa K933000
display id: BBa_K933000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: two start codons coding for RFP and sfGFP reporters
creator: Hannah Jepsen-Burger
created: 2012-09-30T11:00:00Z
modified: 2015-05-08T01:13:47Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1198_
m user id: 0
owner id: 13034
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part was used in a design that tests multiple start codons. The first start codon codes for RFP. The second start codon codes for sfGFP. They are 7 nucleotides apart on the DNA strand. The ribosome binding site translation initiation rate determines which protein is expressed. The part has two restriction enzyme sites flanking the RBS and promoter regions, so varying RBS strengths may be tested.
mutable notes: This design used gBlocks synthesized by IDT. Several CBAR reactions were necessary to achieve the construct.
mutable provenance: This part contains no direct BioBrick parts. It was assembled from several pieces of plasmid DNA in the Salis Lab at Penn State. The dRBS1 vector and pBAC reporter components were assembled from PCR-amplified fragments.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: weak RBS
sequence annotation: start codon RFP (ORF 0)
sequence annotation: double terminator
sequence annotation: sfGFP reporter
sequence annotation: start codon sfGFP (ORF +1)
sequence annotation: 1N break in reference frame
sequence annotation: 41
sequence: https://synbiohub.org/public/igem/BBa_K933000_sequence/1

weak RBS
type: SequenceAnnotation
persistent identity: annotation2211994
display id: annotation2211994
version: 1
top level: BBa_K933000
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K933000/annotation2211994/range2211994/1

https://synbiohub.org/public/igem/BBa_K933000/annotation2211994/range2211994/1
type: Range
persistent identity: range2211994
display id: range2211994
version: 1
direction: direction/forward
top level: BBa_K933000
start: 1
end: 28
orientation: inline

start codon RFP (ORF 0)
type: SequenceAnnotation
persistent identity: annotation2211995
display id: annotation2211995
version: 1
top level: BBa_K933000
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K933000/annotation2211995/range2211995/1

https://synbiohub.org/public/igem/BBa_K933000/annotation2211995/range2211995/1
type: Range
persistent identity: range2211995
display id: range2211995
version: 1
direction: direction/forward
top level: BBa_K933000
start: 29
end: 30
orientation: inline

double terminator
type: SequenceAnnotation
persistent identity: annotation2212000
display id: annotation2212000
version: 1
top level: BBa_K933000
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K933000/annotation2212000/range2212000/1

https://synbiohub.org/public/igem/BBa_K933000/annotation2212000/range2212000/1
type: Range
persistent identity: range2212000
display id: range2212000
version: 1
direction: direction/forward
top level: BBa_K933000
start: 1461
end: 1589
orientation: inline

sfGFP reporter
type: SequenceAnnotation
persistent identity: annotation2211999
display id: annotation2211999
version: 1
top level: BBa_K933000
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K933000/annotation2211999/range2211999/1

https://synbiohub.org/public/igem/BBa_K933000/annotation2211999/range2211999/1
type: Range
persistent identity: range2211999
display id: range2211999
version: 1
direction: direction/forward
top level: BBa_K933000
start: 711
end: 1427
orientation: inline

start codon sfGFP (ORF +1)
type: SequenceAnnotation
persistent identity: annotation2211996
display id: annotation2211996
version: 1
top level: BBa_K933000
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K933000/annotation2211996/range2211996/1

https://synbiohub.org/public/igem/BBa_K933000/annotation2211996/range2211996/1
type: Range
persistent identity: range2211996
display id: range2211996
version: 1
direction: direction/forward
top level: BBa_K933000
start: 39
end: 40
orientation: inline

1N break in reference frame
type: SequenceAnnotation
persistent identity: annotation2211998
display id: annotation2211998
version: 1
top level: BBa_K933000
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K933000/annotation2211998/range2211998/1

https://synbiohub.org/public/igem/BBa_K933000/annotation2211998/range2211998/1
type: Range
persistent identity: range2211998
display id: range2211998
version: 1
direction: direction/forward
top level: BBa_K933000
start: 710
end: 710
orientation: inline

41
type: SequenceAnnotation
persistent identity: annotation2211997
display id: annotation2211997
version: 1
top level: BBa_K933000
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K933000/annotation2211997/range2211997/1

https://synbiohub.org/public/igem/BBa_K933000/annotation2211997/range2211997/1
type: Range
persistent identity: range2211997
display id: range2211997
version: 1
direction: direction/forward
top level: BBa_K933000
start: 41
end: 709
orientation: inline

BBa_K1031804
type: ComponentDefinition
persistent identity: BBa K1031804
display id: BBa_K1031804
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Pu-B0032-sfGFP-Terminator (XylR)
creator: He Shuaixin
created: 2013-09-08T11:00:00Z
modified: 2015-05-08T01:08:48Z
discontinued: false
dominant: false
group u list: _1338_
m user id: 0
owner id: 15843
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Pu-32-sfGFP-TT (XylR)
mutable notes: molecular cloning
mutable provenance: synthesis, lab made
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: B0015
sequence annotation: B0032
sequence annotation: sfGFP
sequence annotation: Pu (XylR)
sequence: https://synbiohub.org/public/igem/BBa_K1031804_sequence/1

B0015
type: SequenceAnnotation
persistent identity: annotation2362534
display id: annotation2362534
version: 1
top level: BBa_K1031804
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1031804/annotation2362534/range2362534/1

https://synbiohub.org/public/igem/BBa_K1031804/annotation2362534/range2362534/1
type: Range
persistent identity: range2362534
display id: range2362534
version: 1
direction: direction/forward
top level: BBa_K1031804
start: 935
end: 1063
orientation: inline

B0032
type: SequenceAnnotation
persistent identity: annotation2362532
display id: annotation2362532
version: 1
top level: BBa_K1031804
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1031804/annotation2362532/range2362532/1

https://synbiohub.org/public/igem/BBa_K1031804/annotation2362532/range2362532/1
type: Range
persistent identity: range2362532
display id: range2362532
version: 1
direction: direction/forward
top level: BBa_K1031804
start: 187
end: 199
orientation: inline

sfGFP
type: SequenceAnnotation
persistent identity: annotation2362533
display id: annotation2362533
version: 1
top level: BBa_K1031804
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1031804/annotation2362533/range2362533/1

https://synbiohub.org/public/igem/BBa_K1031804/annotation2362533/range2362533/1
type: Range
persistent identity: range2362533
display id: range2362533
version: 1
direction: direction/forward
top level: BBa_K1031804
start: 206
end: 928
orientation: inline

Pu (XylR)
type: SequenceAnnotation
persistent identity: annotation2362531
display id: annotation2362531
version: 1
top level: BBa_K1031804
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1031804/annotation2362531/range2362531/1

https://synbiohub.org/public/igem/BBa_K1031804/annotation2362531/range2362531/1
type: Range
persistent identity: range2362531
display id: range2362531
version: 1
direction: direction/forward
top level: BBa_K1031804
start: 1
end: 180
orientation: inline

Zwf-Device
type: ComponentDefinition
persistent identity: BBa K1840007
display id: BBa_K1840007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Device: TT-zwf-mCherry
creator: Julia Anna Adrian
created: 2015-09-17T11:00:00Z
modified: 2015-09-18T02:16:57Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2266_
m user id: 26543
owner id: 26543
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This device acts as a detector for glucose (and certain derivatives of it). The double transcription terminator ensures, that only the gene downstream of the promotor zwf is influenced by it. The zwf promotor (BBa_K1840002) is contained in the genome of Pseudomonas putida and is situated in front of the enzyme zwf (glucose 6-phosphate dehydrogenase). The repressor HexR can bind the zwf promotor sequence and thus prevent the transcription of the zwf gene. In case the glucose derivative 2-Keto-3-deoxy-6-phosphogluconate is present, it binds to the promotor and leads to a subsequent conformational change. Hence, the repressor can no longer bind and the gene(s) downstream can be transcribed. In our device, the downstream gene is mCherry, codon optimized for Pseudomonas (BBa_K1840004). Therefore, in the presence of glucose, mCherry is expressed. In various experiments, we could see that this device is in deed working.
mutable notes: As promotor sequence, we assumed all DNA in front of the zwf gene up to the next gene, coding for the repressor HexR. Since it is encoded on the opposite strand, there is the possibility, that zwf functions as a promotor for this gene as well. Therefore the double terminator was needed.
mutable provenance: The double terminator was already registered in the iGEM catalogue. The zwf promotor comes from the genome of Pseudomonas putida. The mCherry is codon optimized for expression in Pseudomonas.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1840002
component: BBa_B0014
component: BBa_K1840004
sequence annotation: BBa_B0014
sequence annotation: BBa_K1840002
sequence annotation: BBa_K1840004
sequence: https://synbiohub.org/public/igem/BBa_K1840007_sequence/1

BBa_K1840002
type: Component
persistent identity: component2473635
display id: component2473635
version: 1
top level: Zwf-Device
access: public
definition: https://synbiohub.org/public/igem/BBa_K1840002/1

BBa_B0014
type: Component
persistent identity: component2473634
display id: component2473634
version: 1
top level: Zwf-Device
access: public
definition: BBa_B0014

BBa_K1840004
type: Component
persistent identity: component2473636
display id: component2473636
version: 1
top level: Zwf-Device
access: public
definition: https://synbiohub.org/public/igem/BBa_K1840004/1

BBa_B0014
type: SequenceAnnotation
persistent identity: annotation2473634
display id: annotation2473634
version: 1
top level: Zwf-Device
location: https://synbiohub.org/public/igem/BBa_K1840007/annotation2473634/range2473634/1
component: BBa_B0014

https://synbiohub.org/public/igem/BBa_K1840007/annotation2473634/range2473634/1
type: Range
persistent identity: range2473634
display id: range2473634
version: 1
direction: direction/reverse
top level: Zwf-Device
start: 1
end: 95
orientation: inline

BBa_K1840002
type: SequenceAnnotation
persistent identity: annotation2473635
display id: annotation2473635
version: 1
top level: Zwf-Device
location: https://synbiohub.org/public/igem/BBa_K1840007/annotation2473635/range2473635/1
component: BBa_K1840002

https://synbiohub.org/public/igem/BBa_K1840007/annotation2473635/range2473635/1
type: Range
persistent identity: range2473635
display id: range2473635
version: 1
direction: direction/reverse
top level: Zwf-Device
start: 104
end: 305
orientation: inline

BBa_K1840004
type: SequenceAnnotation
persistent identity: annotation2473636
display id: annotation2473636
version: 1
top level: Zwf-Device
location: https://synbiohub.org/public/igem/BBa_K1840007/annotation2473636/range2473636/1
component: BBa_K1840004

https://synbiohub.org/public/igem/BBa_K1840007/annotation2473636/range2473636/1
type: Range
persistent identity: range2473636
display id: range2473636
version: 1
direction: direction/reverse
top level: Zwf-Device
start: 312
end: 1025
orientation: inline

BBa_K1031625
type: ComponentDefinition
persistent identity: BBa K1031625
display id: BBa_K1031625
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: J23117-NahF-Terminator
creator: He Shuaixin
created: 2013-09-08T11:00:00Z
modified: 2015-05-08T01:08:47Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1338_
m user id: 0
owner id: 15843
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: 117-NahF-TT
mutable notes: molecular cloning
mutable provenance: synthesis Pseudomonas
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: B0015
sequence annotation: NahF
sequence annotation: J23117
sequence: https://synbiohub.org/public/igem/BBa_K1031625_sequence/1

B0015
type: SequenceAnnotation
persistent identity: annotation2361916
display id: annotation2361916
version: 1
top level: BBa_K1031625
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1031625/annotation2361916/range2361916/1

https://synbiohub.org/public/igem/BBa_K1031625/annotation2361916/range2361916/1
type: Range
persistent identity: range2361916
display id: range2361916
version: 1
direction: direction/forward
top level: BBa_K1031625
start: 1520
end: 1648
orientation: inline

NahF
type: SequenceAnnotation
persistent identity: annotation2361915
display id: annotation2361915
version: 1
top level: BBa_K1031625
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1031625/annotation2361915/range2361915/1

https://synbiohub.org/public/igem/BBa_K1031625/annotation2361915/range2361915/1
type: Range
persistent identity: range2361915
display id: range2361915
version: 1
direction: direction/forward
top level: BBa_K1031625
start: 62
end: 1513
orientation: inline

J23117
type: SequenceAnnotation
persistent identity: annotation2361913
display id: annotation2361913
version: 1
top level: BBa_K1031625
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1031625/annotation2361913/range2361913/1

https://synbiohub.org/public/igem/BBa_K1031625/annotation2361913/range2361913/1
type: Range
persistent identity: range2361913
display id: range2361913
version: 1
direction: direction/forward
top level: BBa_K1031625
start: 1
end: 35
orientation: inline

BBa_K1031624
type: ComponentDefinition
persistent identity: BBa K1031624
display id: BBa_K1031624
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: J23114-NahF-Terminator
creator: He Shuaixin
created: 2013-09-08T11:00:00Z
modified: 2015-05-08T01:08:47Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1338_
m user id: 0
owner id: 15843
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: 114-NahF-TT
mutable notes: molecular cloning
mutable provenance: synthesis Pseudomonas
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: J23114
sequence annotation: NahF
sequence annotation: B0015
sequence: https://synbiohub.org/public/igem/BBa_K1031624_sequence/1

J23114
type: SequenceAnnotation
persistent identity: annotation2361903
display id: annotation2361903
version: 1
top level: BBa_K1031624
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1031624/annotation2361903/range2361903/1

https://synbiohub.org/public/igem/BBa_K1031624/annotation2361903/range2361903/1
type: Range
persistent identity: range2361903
display id: range2361903
version: 1
direction: direction/forward
top level: BBa_K1031624
start: 1
end: 35
orientation: inline

NahF
type: SequenceAnnotation
persistent identity: annotation2361904
display id: annotation2361904
version: 1
top level: BBa_K1031624
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1031624/annotation2361904/range2361904/1

https://synbiohub.org/public/igem/BBa_K1031624/annotation2361904/range2361904/1
type: Range
persistent identity: range2361904
display id: range2361904
version: 1
direction: direction/forward
top level: BBa_K1031624
start: 62
end: 1513
orientation: inline

B0015
type: SequenceAnnotation
persistent identity: annotation2361905
display id: annotation2361905
version: 1
top level: BBa_K1031624
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1031624/annotation2361905/range2361905/1

https://synbiohub.org/public/igem/BBa_K1031624/annotation2361905/range2361905/1
type: Range
persistent identity: range2361905
display id: range2361905
version: 1
direction: direction/forward
top level: BBa_K1031624
start: 1520
end: 1648
orientation: inline

BBa_K1031923
type: ComponentDefinition
persistent identity: BBa K1031923
display id: BBa_K1031923
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: J23109-XylB-Terminator
creator: He Shuaixin
created: 2013-10-21T11:00:00Z
modified: 2015-05-08T01:08:48Z
discontinued: false
dominant: false
group u list: _1338_
m user id: 0
owner id: 15843
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: J23109-XylB-Terminator
mutable notes: molecular cloning
mutable provenance: Pseudomonas.putida
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: B0015
sequence annotation: J23109
sequence annotation: XylB
sequence: https://synbiohub.org/public/igem/BBa_K1031923_sequence/1

B0015
type: SequenceAnnotation
persistent identity: annotation2370041
display id: annotation2370041
version: 1
top level: BBa_K1031923
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1031923/annotation2370041/range2370041/1

https://synbiohub.org/public/igem/BBa_K1031923/annotation2370041/range2370041/1
type: Range
persistent identity: range2370041
display id: range2370041
version: 1
direction: direction/forward
top level: BBa_K1031923
start: 1176
end: 1304
orientation: inline

J23109
type: SequenceAnnotation
persistent identity: annotation2370039
display id: annotation2370039
version: 1
top level: BBa_K1031923
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1031923/annotation2370039/range2370039/1

https://synbiohub.org/public/igem/BBa_K1031923/annotation2370039/range2370039/1
type: Range
persistent identity: range2370039
display id: range2370039
version: 1
direction: direction/forward
top level: BBa_K1031923
start: 1
end: 35
orientation: inline

XylB
type: SequenceAnnotation
persistent identity: annotation2370040
display id: annotation2370040
version: 1
top level: BBa_K1031923
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1031923/annotation2370040/range2370040/1

https://synbiohub.org/public/igem/BBa_K1031923/annotation2370040/range2370040/1
type: Range
persistent identity: range2370040
display id: range2370040
version: 1
direction: direction/forward
top level: BBa_K1031923
start: 63
end: 1169
orientation: inline

BBa_K1031114
type: ComponentDefinition
persistent identity: BBa K1031114
display id: BBa_K1031114
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Po-B0032-sfGFP (CapR)
creator: He Shuaixin
created: 2013-09-08T11:00:00Z
modified: 2015-05-08T01:08:47Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1338_
m user id: 0
owner id: 15843
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Po-32-sfGFP (CapR)
mutable notes: molecular cloning
mutable provenance: synthesis, lab made
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: sfGFP
sequence annotation: B0032
sequence annotation: B0015
sequence annotation: Po (CapR)
sequence: https://synbiohub.org/public/igem/BBa_K1031114_sequence/1

sfGFP
type: SequenceAnnotation
persistent identity: annotation2362070
display id: annotation2362070
version: 1
top level: BBa_K1031114
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1031114/annotation2362070/range2362070/1

https://synbiohub.org/public/igem/BBa_K1031114/annotation2362070/range2362070/1
type: Range
persistent identity: range2362070
display id: range2362070
version: 1
direction: direction/forward
top level: BBa_K1031114
start: 196
end: 918
orientation: inline

B0032
type: SequenceAnnotation
persistent identity: annotation2362069
display id: annotation2362069
version: 1
top level: BBa_K1031114
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1031114/annotation2362069/range2362069/1

https://synbiohub.org/public/igem/BBa_K1031114/annotation2362069/range2362069/1
type: Range
persistent identity: range2362069
display id: range2362069
version: 1
direction: direction/forward
top level: BBa_K1031114
start: 177
end: 189
orientation: inline

B0015
type: SequenceAnnotation
persistent identity: annotation2362071
display id: annotation2362071
version: 1
top level: BBa_K1031114
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1031114/annotation2362071/range2362071/1

https://synbiohub.org/public/igem/BBa_K1031114/annotation2362071/range2362071/1
type: Range
persistent identity: range2362071
display id: range2362071
version: 1
direction: direction/forward
top level: BBa_K1031114
start: 925
end: 1053
orientation: inline

Po (CapR)
type: SequenceAnnotation
persistent identity: annotation2362068
display id: annotation2362068
version: 1
top level: BBa_K1031114
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1031114/annotation2362068/range2362068/1

https://synbiohub.org/public/igem/BBa_K1031114/annotation2362068/range2362068/1
type: Range
persistent identity: range2362068
display id: range2362068
version: 1
direction: direction/forward
top level: BBa_K1031114
start: 1
end: 170
orientation: inline

BBa_K1031222
type: ComponentDefinition
persistent identity: BBa K1031222
display id: BBa_K1031222
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Po-B0032-sfGFP-Terminator (DmpR)
creator: He Shuaixin
created: 2013-09-08T11:00:00Z
modified: 2015-05-08T01:08:47Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1338_
m user id: 0
owner id: 15843
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: Po-32-sfGFP-TT (DmpR)
mutable notes: molecular cloning
mutable provenance: synthesis, lab made
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: po promoter
sequence annotation: B0032
sequence annotation: B0015
sequence annotation: sfGFP
sequence: https://synbiohub.org/public/igem/BBa_K1031222_sequence/1

po promoter
type: SequenceAnnotation
persistent identity: annotation2354354
display id: annotation2354354
version: 1
top level: BBa_K1031222
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1031222/annotation2354354/range2354354/1

https://synbiohub.org/public/igem/BBa_K1031222/annotation2354354/range2354354/1
type: Range
persistent identity: range2354354
display id: range2354354
version: 1
direction: direction/forward
top level: BBa_K1031222
start: 1
end: 171
orientation: inline

B0032
type: SequenceAnnotation
persistent identity: annotation2354355
display id: annotation2354355
version: 1
top level: BBa_K1031222
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1031222/annotation2354355/range2354355/1

https://synbiohub.org/public/igem/BBa_K1031222/annotation2354355/range2354355/1
type: Range
persistent identity: range2354355
display id: range2354355
version: 1
direction: direction/forward
top level: BBa_K1031222
start: 180
end: 182
orientation: inline

B0015
type: SequenceAnnotation
persistent identity: annotation2354357
display id: annotation2354357
version: 1
top level: BBa_K1031222
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1031222/annotation2354357/range2354357/1

https://synbiohub.org/public/igem/BBa_K1031222/annotation2354357/range2354357/1
type: Range
persistent identity: range2354357
display id: range2354357
version: 1
direction: direction/forward
top level: BBa_K1031222
start: 930
end: 1058
orientation: inline

sfGFP
type: SequenceAnnotation
persistent identity: annotation2354356
display id: annotation2354356
version: 1
top level: BBa_K1031222
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1031222/annotation2354356/range2354356/1

https://synbiohub.org/public/igem/BBa_K1031222/annotation2354356/range2354356/1
type: Range
persistent identity: range2354356
display id: range2354356
version: 1
direction: direction/forward
top level: BBa_K1031222
start: 199
end: 921
orientation: inline

BBa_B0024
type: ComponentDefinition
persistent identity: BBa B0024
display id: BBa_B0024
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: double terminator (B0012-B0011), reversed
creator: Caitlin Conboy
created: 2003-12-02T12:00:00Z
modified: 2015-08-31T04:07:20Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence: https://synbiohub.org/public/igem/BBa_B0024_sequence/1

BBa_K2122100
type: ComponentDefinition
persistent identity: BBa K2122100
display id: BBa_K2122100
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Device expressing the Gb3 Synthase enzyme under control of a constitutive promoter
creator: Sannia Farrukh
created: 2016-09-28T11:00:00Z
modified: 2016-10-18T01:30:37Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2591_
m user id: 32074
owner id: 32074
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This part will code for Gb3 synthase. Gb3 synthase in return makes Gb3 which is a receptor for Shiga-like toxin (stx 1&2). Shiga-like toxins are exotoxins, which consist of a toxic enzymatic A subunit and a cell-binding B subunit. The latter binds to a globotriaosylceramide (Gb3) receptor, expressed on the surface of the target cells in humans. This interaction is responsible for the toxin's entry into the host cell. When the receptor is exposed to the food sample, the SLT subunit B, if present, will bind to Gb3.
mutable notes: A constitutive promoter was added to the sequence
mutable provenance: Coding Sequence from GenBank
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_B0014
component: BBa_K2122150
component: BBa_B0034
component: BBa_J23100
sequence annotation: BBa_B0014
sequence annotation: BBa_B0034
sequence annotation: BBa_J23100
sequence annotation: BBa_K2122150
sequence: https://synbiohub.org/public/igem/BBa_K2122100_sequence/1

BBa_B0014
type: Component
persistent identity: component2518023
display id: component2518023
version: 1
top level: BBa_K2122100
access: public
definition: BBa_B0014

BBa_K2122150
type: Component
persistent identity: component2518016
display id: component2518016
version: 1
top level: BBa_K2122100
access: public
definition: https://synbiohub.org/public/igem/BBa_K2122150/1

BBa_B0034
type: Component
persistent identity: component2518015
display id: component2518015
version: 1
top level: BBa_K2122100
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_J23100
type: Component
persistent identity: component2518013
display id: component2518013
version: 1
top level: BBa_K2122100
access: public
definition: https://synbiohub.org/public/igem/BBa_J23100/1

BBa_B0014
type: SequenceAnnotation
persistent identity: annotation2518023
display id: annotation2518023
version: 1
top level: BBa_K2122100
location: https://synbiohub.org/public/igem/BBa_K2122100/annotation2518023/range2518023/1
component: BBa_B0014

https://synbiohub.org/public/igem/BBa_K2122100/annotation2518023/range2518023/1
type: Range
persistent identity: range2518023
display id: range2518023
version: 1
direction: direction/reverse
top level: BBa_K2122100
start: 1132
end: 1226
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2518015
display id: annotation2518015
version: 1
top level: BBa_K2122100
location: https://synbiohub.org/public/igem/BBa_K2122100/annotation2518015/range2518015/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K2122100/annotation2518015/range2518015/1
type: Range
persistent identity: range2518015
display id: range2518015
version: 1
direction: direction/reverse
top level: BBa_K2122100
start: 44
end: 55
orientation: inline

BBa_J23100
type: SequenceAnnotation
persistent identity: annotation2518013
display id: annotation2518013
version: 1
top level: BBa_K2122100
location: https://synbiohub.org/public/igem/BBa_K2122100/annotation2518013/range2518013/1
component: BBa_J23100

https://synbiohub.org/public/igem/BBa_K2122100/annotation2518013/range2518013/1
type: Range
persistent identity: range2518013
display id: range2518013
version: 1
direction: direction/reverse
top level: BBa_K2122100
start: 1
end: 35
orientation: inline

BBa_K2122150
type: SequenceAnnotation
persistent identity: annotation2518016
display id: annotation2518016
version: 1
top level: BBa_K2122100
location: https://synbiohub.org/public/igem/BBa_K2122100/annotation2518016/range2518016/1
component: BBa_K2122150

https://synbiohub.org/public/igem/BBa_K2122100/annotation2518016/range2518016/1
type: Range
persistent identity: range2518016
display id: range2518016
version: 1
direction: direction/reverse
top level: BBa_K2122100
start: 62
end: 1123
orientation: inline

BBa_K1031302
type: ComponentDefinition
persistent identity: BBa K1031302
display id: BBa_K1031302
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Pc-B0032-sfGFP-Terminator (HbpR)
creator: He Shuaixin
created: 2013-09-08T11:00:00Z
modified: 2015-05-08T01:08:47Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1338_
m user id: 0
owner id: 15843
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Ph-32-sfGFP-TT (HbpR)
mutable notes: molecular cloning
mutable provenance: synthesis, lab made
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: Ph
sequence annotation: BBa_B0032
sequence annotation: BBa_B0015
sequence annotation: sfGFP
sequence: https://synbiohub.org/public/igem/BBa_K1031302_sequence/1

Ph
type: SequenceAnnotation
persistent identity: annotation2360436
display id: annotation2360436
version: 1
top level: BBa_K1031302
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1031302/annotation2360436/range2360436/1

https://synbiohub.org/public/igem/BBa_K1031302/annotation2360436/range2360436/1
type: Range
persistent identity: range2360436
display id: range2360436
version: 1
direction: direction/forward
top level: BBa_K1031302
start: 1
end: 229
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation2360437
display id: annotation2360437
version: 1
top level: BBa_K1031302
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1031302/annotation2360437/range2360437/1

https://synbiohub.org/public/igem/BBa_K1031302/annotation2360437/range2360437/1
type: Range
persistent identity: range2360437
display id: range2360437
version: 1
direction: direction/forward
top level: BBa_K1031302
start: 236
end: 247
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2360439
display id: annotation2360439
version: 1
top level: BBa_K1031302
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1031302/annotation2360439/range2360439/1

https://synbiohub.org/public/igem/BBa_K1031302/annotation2360439/range2360439/1
type: Range
persistent identity: range2360439
display id: range2360439
version: 1
direction: direction/forward
top level: BBa_K1031302
start: 983
end: 1111
orientation: inline

sfGFP
type: SequenceAnnotation
persistent identity: annotation2360438
display id: annotation2360438
version: 1
top level: BBa_K1031302
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1031302/annotation2360438/range2360438/1

https://synbiohub.org/public/igem/BBa_K1031302/annotation2360438/range2360438/1
type: Range
persistent identity: range2360438
display id: range2360438
version: 1
direction: direction/forward
top level: BBa_K1031302
start: 254
end: 976
orientation: inline

BBa_K1031803
type: ComponentDefinition
persistent identity: BBa K1031803
display id: BBa_K1031803
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Pu-B0031-sfGFP-Terminator (XylR)
creator: He Shuaixin
created: 2013-09-08T11:00:00Z
modified: 2015-05-08T01:08:48Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1338_
m user id: 0
owner id: 15843
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Pu-31-sfGFP-TT (XylR)
mutable notes: molecular cloning
mutable provenance: synthesis, lab made
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: B0031
sequence annotation: Pu (XylR)
sequence annotation: B0015
sequence annotation: sfGFP
sequence: https://synbiohub.org/public/igem/BBa_K1031803_sequence/1

B0031
type: SequenceAnnotation
persistent identity: annotation2362526
display id: annotation2362526
version: 1
top level: BBa_K1031803
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1031803/annotation2362526/range2362526/1

https://synbiohub.org/public/igem/BBa_K1031803/annotation2362526/range2362526/1
type: Range
persistent identity: range2362526
display id: range2362526
version: 1
direction: direction/forward
top level: BBa_K1031803
start: 187
end: 200
orientation: inline

Pu (XylR)
type: SequenceAnnotation
persistent identity: annotation2362525
display id: annotation2362525
version: 1
top level: BBa_K1031803
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1031803/annotation2362525/range2362525/1

https://synbiohub.org/public/igem/BBa_K1031803/annotation2362525/range2362525/1
type: Range
persistent identity: range2362525
display id: range2362525
version: 1
direction: direction/forward
top level: BBa_K1031803
start: 1
end: 180
orientation: inline

B0015
type: SequenceAnnotation
persistent identity: annotation2362528
display id: annotation2362528
version: 1
top level: BBa_K1031803
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1031803/annotation2362528/range2362528/1

https://synbiohub.org/public/igem/BBa_K1031803/annotation2362528/range2362528/1
type: Range
persistent identity: range2362528
display id: range2362528
version: 1
direction: direction/forward
top level: BBa_K1031803
start: 936
end: 1064
orientation: inline

sfGFP
type: SequenceAnnotation
persistent identity: annotation2362527
display id: annotation2362527
version: 1
top level: BBa_K1031803
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1031803/annotation2362527/range2362527/1

https://synbiohub.org/public/igem/BBa_K1031803/annotation2362527/range2362527/1
type: Range
persistent identity: range2362527
display id: range2362527
version: 1
direction: direction/forward
top level: BBa_K1031803
start: 221
end: 929
orientation: inline

BBa_K1031510
type: ComponentDefinition
persistent identity: BBa K1031510
display id: BBa_K1031510
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: HpaR-Terminator
creator: He Shuaixin
created: 2013-09-08T11:00:00Z
modified: 2015-05-08T01:08:47Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1338_
m user id: 0
owner id: 15843
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: HpaR-TT
mutable notes: molecular cloning
mutable provenance: PCR genome E.coli
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: HpaR
sequence annotation: BBa_B0034
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_K1031510_sequence/1

HpaR
type: SequenceAnnotation
persistent identity: annotation2357472
display id: annotation2357472
version: 1
top level: BBa_K1031510
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1031510/annotation2357472/range2357472/1

https://synbiohub.org/public/igem/BBa_K1031510/annotation2357472/range2357472/1
type: Range
persistent identity: range2357472
display id: range2357472
version: 1
direction: direction/forward
top level: BBa_K1031510
start: 19
end: 465
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2357469
display id: annotation2357469
version: 1
top level: BBa_K1031510
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1031510/annotation2357469/range2357469/1

https://synbiohub.org/public/igem/BBa_K1031510/annotation2357469/range2357469/1
type: Range
persistent identity: range2357469
display id: range2357469
version: 1
direction: direction/forward
top level: BBa_K1031510
start: 1
end: 12
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2357473
display id: annotation2357473
version: 1
top level: BBa_K1031510
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1031510/annotation2357473/range2357473/1

https://synbiohub.org/public/igem/BBa_K1031510/annotation2357473/range2357473/1
type: Range
persistent identity: range2357473
display id: range2357473
version: 1
direction: direction/forward
top level: BBa_K1031510
start: 474
end: 602
orientation: inline

BBa_K1031941
type: ComponentDefinition
persistent identity: BBa K1031941
display id: BBa_K1031941
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Pm-B0034-eGFP (XylS)
creator: He Shuaixin
created: 2013-10-20T11:00:00Z
modified: 2015-05-08T01:08:48Z
discontinued: false
dominant: false
group u list: _1338_
m user id: 0
owner id: 15843
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Pm-B0034-eGFP (XylS)
mutable notes: molecular cloning
mutable provenance: Pseudomonas.putida
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: eGFP
sequence annotation: Pm
sequence annotation: B0015
sequence annotation: B0034
sequence: https://synbiohub.org/public/igem/BBa_K1031941_sequence/1

eGFP
type: SequenceAnnotation
persistent identity: annotation2370034
display id: annotation2370034
version: 1
top level: BBa_K1031941
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1031941/annotation2370034/range2370034/1

https://synbiohub.org/public/igem/BBa_K1031941/annotation2370034/range2370034/1
type: Range
persistent identity: range2370034
display id: range2370034
version: 1
direction: direction/forward
top level: BBa_K1031941
start: 107
end: 977
orientation: inline

Pm
type: SequenceAnnotation
persistent identity: annotation2370032
display id: annotation2370032
version: 1
top level: BBa_K1031941
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1031941/annotation2370032/range2370032/1

https://synbiohub.org/public/igem/BBa_K1031941/annotation2370032/range2370032/1
type: Range
persistent identity: range2370032
display id: range2370032
version: 1
direction: direction/forward
top level: BBa_K1031941
start: 1
end: 88
orientation: inline

B0015
type: SequenceAnnotation
persistent identity: annotation2370035
display id: annotation2370035
version: 1
top level: BBa_K1031941
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1031941/annotation2370035/range2370035/1

https://synbiohub.org/public/igem/BBa_K1031941/annotation2370035/range2370035/1
type: Range
persistent identity: range2370035
display id: range2370035
version: 1
direction: direction/forward
top level: BBa_K1031941
start: 984
end: 1112
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation2370033
display id: annotation2370033
version: 1
top level: BBa_K1031941
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1031941/annotation2370033/range2370033/1

https://synbiohub.org/public/igem/BBa_K1031941/annotation2370033/range2370033/1
type: Range
persistent identity: range2370033
display id: range2370033
version: 1
direction: direction/forward
top level: BBa_K1031941
start: 89
end: 101
orientation: inline

BBa_K1031224
type: ComponentDefinition
persistent identity: BBa K1031224
display id: BBa_K1031224
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Po-B0034-sfGFP-Terminator (DmpR)
creator: He Shuaixin
created: 2013-09-08T11:00:00Z
modified: 2015-05-08T01:08:47Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1338_
m user id: 0
owner id: 15843
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Po-34-sfGFP-TT (DmpR)
mutable notes: molecular cloning
mutable provenance: synthesis, lab made
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: B0015
sequence annotation: po promoter
sequence annotation: B0034
sequence annotation: sfGFP
sequence: https://synbiohub.org/public/igem/BBa_K1031224_sequence/1

B0015
type: SequenceAnnotation
persistent identity: annotation2354393
display id: annotation2354393
version: 1
top level: BBa_K1031224
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1031224/annotation2354393/range2354393/1

https://synbiohub.org/public/igem/BBa_K1031224/annotation2354393/range2354393/1
type: Range
persistent identity: range2354393
display id: range2354393
version: 1
direction: direction/forward
top level: BBa_K1031224
start: 929
end: 1057
orientation: inline

po promoter
type: SequenceAnnotation
persistent identity: annotation2354390
display id: annotation2354390
version: 1
top level: BBa_K1031224
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1031224/annotation2354390/range2354390/1

https://synbiohub.org/public/igem/BBa_K1031224/annotation2354390/range2354390/1
type: Range
persistent identity: range2354390
display id: range2354390
version: 1
direction: direction/forward
top level: BBa_K1031224
start: 1
end: 171
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation2354391
display id: annotation2354391
version: 1
top level: BBa_K1031224
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1031224/annotation2354391/range2354391/1

https://synbiohub.org/public/igem/BBa_K1031224/annotation2354391/range2354391/1
type: Range
persistent identity: range2354391
display id: range2354391
version: 1
direction: direction/forward
top level: BBa_K1031224
start: 180
end: 191
orientation: inline

sfGFP
type: SequenceAnnotation
persistent identity: annotation2354392
display id: annotation2354392
version: 1
top level: BBa_K1031224
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1031224/annotation2354392/range2354392/1

https://synbiohub.org/public/igem/BBa_K1031224/annotation2354392/range2354392/1
type: Range
persistent identity: range2354392
display id: range2354392
version: 1
direction: direction/forward
top level: BBa_K1031224
start: 198
end: 920
orientation: inline

BBa_K1031111
type: ComponentDefinition
persistent identity: BBa K1031111
display id: BBa_K1031111
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: J23109-CapR-Terminator
creator: He Shuaixin
created: 2013-09-08T11:00:00Z
modified: 2015-05-08T01:08:47Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1338_
m user id: 0
owner id: 15843
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: 109-CapR-TT
mutable notes: molecular cloning
mutable provenance: synthesis Pseudomonas putida
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: B0015
sequence annotation: J23109
sequence annotation: CapR
sequence: https://synbiohub.org/public/igem/BBa_K1031111_sequence/1

B0015
type: SequenceAnnotation
persistent identity: annotation2361311
display id: annotation2361311
version: 1
top level: BBa_K1031111
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1031111/annotation2361311/range2361311/1

https://synbiohub.org/public/igem/BBa_K1031111/annotation2361311/range2361311/1
type: Range
persistent identity: range2361311
display id: range2361311
version: 1
direction: direction/forward
top level: BBa_K1031111
start: 1760
end: 1888
orientation: inline

J23109
type: SequenceAnnotation
persistent identity: annotation2361309
display id: annotation2361309
version: 1
top level: BBa_K1031111
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1031111/annotation2361309/range2361309/1

https://synbiohub.org/public/igem/BBa_K1031111/annotation2361309/range2361309/1
type: Range
persistent identity: range2361309
display id: range2361309
version: 1
direction: direction/forward
top level: BBa_K1031111
start: 1
end: 35
orientation: inline

CapR
type: SequenceAnnotation
persistent identity: annotation2361310
display id: annotation2361310
version: 1
top level: BBa_K1031111
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1031111/annotation2361310/range2361310/1

https://synbiohub.org/public/igem/BBa_K1031111/annotation2361310/range2361310/1
type: Range
persistent identity: range2361310
display id: range2361310
version: 1
direction: direction/forward
top level: BBa_K1031111
start: 62
end: 1753
orientation: inline

BBa_B0014
type: ComponentDefinition
persistent identity: BBa B0014
display id: BBa_B0014
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: double terminator (B0012-B0011)
creator: Reshma Shetty
created: 2003-07-15T11:00:00Z
modified: 2015-08-31T04:07:20Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Double terminator consisting of BBa_B0012 and BBa_B0011
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000141
role: partType/Terminator
component: BBa_B0011
component: BBa_B0012
sequence annotation: BBa_B0012
sequence annotation: BBa_B0011
sequence: https://synbiohub.org/public/igem/BBa_B0014_sequence/1

BBa_B0011
type: Component
persistent identity: component939311
display id: component939311
version: 1
top level: BBa_B0014
access: public
definition: https://synbiohub.org/public/igem/BBa_B0011/1

BBa_B0012
type: Component
persistent identity: component939303
display id: component939303
version: 1
top level: BBa_B0014
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation939303
display id: annotation939303
version: 1
top level: BBa_B0014
location: https://synbiohub.org/public/igem/BBa_B0014/annotation939303/range939303/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0014/annotation939303/range939303/1
type: Range
persistent identity: range939303
display id: range939303
version: 1
direction: direction/forward
top level: BBa_B0014
start: 1
end: 41
orientation: inline

BBa_B0011
type: SequenceAnnotation
persistent identity: annotation939311
display id: annotation939311
version: 1
top level: BBa_B0014
location: https://synbiohub.org/public/igem/BBa_B0014/annotation939311/range939311/1
component: BBa_B0011

https://synbiohub.org/public/igem/BBa_B0014/annotation939311/range939311/1
type: Range
persistent identity: range939311
display id: range939311
version: 1
direction: direction/forward
top level: BBa_B0014
start: 50
end: 95
orientation: inline

BBa_K1031223
type: ComponentDefinition
persistent identity: BBa K1031223
display id: BBa_K1031223
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Po-B0033-sfGFP-Terminator (DmpR)
creator: He Shuaixin
created: 2013-09-08T11:00:00Z
modified: 2015-05-08T01:08:47Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1338_
m user id: 0
owner id: 15843
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Po-33-sfGFP-TT (DmpR)
mutable notes: molecular cloning
mutable provenance: synthesis, lab made
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: b0033
sequence annotation: B0015
sequence annotation: po promoter
sequence annotation: sfGFP
sequence: https://synbiohub.org/public/igem/BBa_K1031223_sequence/1

b0033
type: SequenceAnnotation
persistent identity: annotation2354359
display id: annotation2354359
version: 1
top level: BBa_K1031223
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1031223/annotation2354359/range2354359/1

https://synbiohub.org/public/igem/BBa_K1031223/annotation2354359/range2354359/1
type: Range
persistent identity: range2354359
display id: range2354359
version: 1
direction: direction/forward
top level: BBa_K1031223
start: 180
end: 190
orientation: inline

B0015
type: SequenceAnnotation
persistent identity: annotation2354361
display id: annotation2354361
version: 1
top level: BBa_K1031223
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1031223/annotation2354361/range2354361/1

https://synbiohub.org/public/igem/BBa_K1031223/annotation2354361/range2354361/1
type: Range
persistent identity: range2354361
display id: range2354361
version: 1
direction: direction/forward
top level: BBa_K1031223
start: 928
end: 1056
orientation: inline

po promoter
type: SequenceAnnotation
persistent identity: annotation2354358
display id: annotation2354358
version: 1
top level: BBa_K1031223
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1031223/annotation2354358/range2354358/1

https://synbiohub.org/public/igem/BBa_K1031223/annotation2354358/range2354358/1
type: Range
persistent identity: range2354358
display id: range2354358
version: 1
direction: direction/forward
top level: BBa_K1031223
start: 1
end: 171
orientation: inline

sfGFP
type: SequenceAnnotation
persistent identity: annotation2354360
display id: annotation2354360
version: 1
top level: BBa_K1031223
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1031223/annotation2354360/range2354360/1

https://synbiohub.org/public/igem/BBa_K1031223/annotation2354360/range2354360/1
type: Range
persistent identity: range2354360
display id: range2354360
version: 1
direction: direction/forward
top level: BBa_K1031223
start: 197
end: 919
orientation: inline

BBa_K1031410
type: ComponentDefinition
persistent identity: BBa K1031410
display id: BBa_K1031410
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: HcaR-Terminator
creator: He Shuaixin
created: 2013-09-08T11:00:00Z
modified: 2015-05-08T01:08:47Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1338_
m user id: 0
owner id: 15843
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: HcaR-TT
mutable notes: molecular cloning
mutable provenance: PCR genome E.coli
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: BBa_B0034
sequence annotation: BBa_B0015
sequence annotation: HcaR
sequence: https://synbiohub.org/public/igem/BBa_K1031410_sequence/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2357450
display id: annotation2357450
version: 1
top level: BBa_K1031410
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1031410/annotation2357450/range2357450/1

https://synbiohub.org/public/igem/BBa_K1031410/annotation2357450/range2357450/1
type: Range
persistent identity: range2357450
display id: range2357450
version: 1
direction: direction/forward
top level: BBa_K1031410
start: 1
end: 12
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2357452
display id: annotation2357452
version: 1
top level: BBa_K1031410
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1031410/annotation2357452/range2357452/1

https://synbiohub.org/public/igem/BBa_K1031410/annotation2357452/range2357452/1
type: Range
persistent identity: range2357452
display id: range2357452
version: 1
direction: direction/forward
top level: BBa_K1031410
start: 918
end: 1046
orientation: inline

HcaR
type: SequenceAnnotation
persistent identity: annotation2357451
display id: annotation2357451
version: 1
top level: BBa_K1031410
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1031410/annotation2357451/range2357451/1

https://synbiohub.org/public/igem/BBa_K1031410/annotation2357451/range2357451/1
type: Range
persistent identity: range2357451
display id: range2357451
version: 1
direction: direction/forward
top level: BBa_K1031410
start: 19
end: 909
orientation: inline

BBa_K1031100
type: ComponentDefinition
persistent identity: BBa K1031100
display id: BBa_K1031100
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CapR-Terminator
creator: He Shuaixin
created: 2013-09-08T11:00:00Z
modified: 2015-05-08T01:08:47Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1338_
m user id: 0
owner id: 15843
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: CapR-TT
mutable notes: Pseudomonas putida
mutable provenance: synthesis
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: B0015
sequence annotation: CapR
sequence: https://synbiohub.org/public/igem/BBa_K1031100_sequence/1

B0015
type: SequenceAnnotation
persistent identity: annotation2361122
display id: annotation2361122
version: 1
top level: BBa_K1031100
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1031100/annotation2361122/range2361122/1

https://synbiohub.org/public/igem/BBa_K1031100/annotation2361122/range2361122/1
type: Range
persistent identity: range2361122
display id: range2361122
version: 1
direction: direction/forward
top level: BBa_K1031100
start: 1699
end: 1827
orientation: inline

CapR
type: SequenceAnnotation
persistent identity: annotation2361063
display id: annotation2361063
version: 1
top level: BBa_K1031100
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1031100/annotation2361063/range2361063/1

https://synbiohub.org/public/igem/BBa_K1031100/annotation2361063/range2361063/1
type: Range
persistent identity: range2361063
display id: range2361063
version: 1
direction: direction/forward
top level: BBa_K1031100
start: 1
end: 1692
orientation: inline

BBa_K1031421
type: ComponentDefinition
persistent identity: BBa K1031421
display id: BBa_K1031421
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ph-B0032-sfGFP-Terminator (HcaR)
creator: He Shuaixin
created: 2013-09-08T11:00:00Z
modified: 2015-05-08T01:08:47Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1338_
m user id: 0
owner id: 15843
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Ph-32-sfGFP-TT (HcaR)
mutable notes: molecular cloning
mutable provenance: synthesis, lab made
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: Ph (HcaR)
sequence annotation: sfGFP
sequence annotation: B0015
sequence annotation: B0032
sequence: https://synbiohub.org/public/igem/BBa_K1031421_sequence/1

Ph (HcaR)
type: SequenceAnnotation
persistent identity: annotation2362372
display id: annotation2362372
version: 1
top level: BBa_K1031421
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1031421/annotation2362372/range2362372/1

https://synbiohub.org/public/igem/BBa_K1031421/annotation2362372/range2362372/1
type: Range
persistent identity: range2362372
display id: range2362372
version: 1
direction: direction/forward
top level: BBa_K1031421
start: 1
end: 74
orientation: inline

sfGFP
type: SequenceAnnotation
persistent identity: annotation2362385
display id: annotation2362385
version: 1
top level: BBa_K1031421
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1031421/annotation2362385/range2362385/1

https://synbiohub.org/public/igem/BBa_K1031421/annotation2362385/range2362385/1
type: Range
persistent identity: range2362385
display id: range2362385
version: 1
direction: direction/forward
top level: BBa_K1031421
start: 100
end: 822
orientation: inline

B0015
type: SequenceAnnotation
persistent identity: annotation2362386
display id: annotation2362386
version: 1
top level: BBa_K1031421
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1031421/annotation2362386/range2362386/1

https://synbiohub.org/public/igem/BBa_K1031421/annotation2362386/range2362386/1
type: Range
persistent identity: range2362386
display id: range2362386
version: 1
direction: direction/forward
top level: BBa_K1031421
start: 829
end: 957
orientation: inline

B0032
type: SequenceAnnotation
persistent identity: annotation2362373
display id: annotation2362373
version: 1
top level: BBa_K1031421
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1031421/annotation2362373/range2362373/1

https://synbiohub.org/public/igem/BBa_K1031421/annotation2362373/range2362373/1
type: Range
persistent identity: range2362373
display id: range2362373
version: 1
direction: direction/forward
top level: BBa_K1031421
start: 81
end: 93
orientation: inline

BBa_K1031301
type: ComponentDefinition
persistent identity: BBa K1031301
display id: BBa_K1031301
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Pc-B0031-sfGFP-Terminator (HbpR)
creator: He Shuaixin
created: 2013-09-08T11:00:00Z
modified: 2015-05-08T01:08:47Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1338_
m user id: 0
owner id: 15843
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Ph-31-sfGFP-TT (HbpR)
mutable notes: molecular cloning
mutable provenance: synthesis, lab made
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: BBa_B0031
sequence annotation: sfGFP
sequence annotation: Ph
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_K1031301_sequence/1

BBa_B0031
type: SequenceAnnotation
persistent identity: annotation2360427
display id: annotation2360427
version: 1
top level: BBa_K1031301
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1031301/annotation2360427/range2360427/1

https://synbiohub.org/public/igem/BBa_K1031301/annotation2360427/range2360427/1
type: Range
persistent identity: range2360427
display id: range2360427
version: 1
direction: direction/forward
top level: BBa_K1031301
start: 236
end: 248
orientation: inline

sfGFP
type: SequenceAnnotation
persistent identity: annotation2360428
display id: annotation2360428
version: 1
top level: BBa_K1031301
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1031301/annotation2360428/range2360428/1

https://synbiohub.org/public/igem/BBa_K1031301/annotation2360428/range2360428/1
type: Range
persistent identity: range2360428
display id: range2360428
version: 1
direction: direction/forward
top level: BBa_K1031301
start: 255
end: 977
orientation: inline

Ph
type: SequenceAnnotation
persistent identity: annotation2360426
display id: annotation2360426
version: 1
top level: BBa_K1031301
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1031301/annotation2360426/range2360426/1

https://synbiohub.org/public/igem/BBa_K1031301/annotation2360426/range2360426/1
type: Range
persistent identity: range2360426
display id: range2360426
version: 1
direction: direction/forward
top level: BBa_K1031301
start: 1
end: 229
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2360429
display id: annotation2360429
version: 1
top level: BBa_K1031301
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1031301/annotation2360429/range2360429/1

https://synbiohub.org/public/igem/BBa_K1031301/annotation2360429/range2360429/1
type: Range
persistent identity: range2360429
display id: range2360429
version: 1
direction: direction/forward
top level: BBa_K1031301
start: 984
end: 1112
orientation: inline

BBa_K1031913
type: ComponentDefinition
persistent identity: BBa K1031913
display id: BBa_K1031913
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: J23109-XylS-Terminator
creator: He Shuaixin
created: 2013-09-08T11:00:00Z
modified: 2015-05-08T01:08:48Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1338_
m user id: 0
owner id: 15843
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: 109-XylS-TT
mutable notes: molecular cloning
mutable provenance: synthesis Pseudomonas
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: J23109
sequence annotation: B0015
sequence annotation: XylS
sequence: https://synbiohub.org/public/igem/BBa_K1031913_sequence/1

J23109
type: SequenceAnnotation
persistent identity: annotation2360169
display id: annotation2360169
version: 1
top level: BBa_K1031913
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1031913/annotation2360169/range2360169/1

https://synbiohub.org/public/igem/BBa_K1031913/annotation2360169/range2360169/1
type: Range
persistent identity: range2360169
display id: range2360169
version: 1
direction: direction/forward
top level: BBa_K1031913
start: 1
end: 35
orientation: inline

B0015
type: SequenceAnnotation
persistent identity: annotation2360171
display id: annotation2360171
version: 1
top level: BBa_K1031913
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1031913/annotation2360171/range2360171/1

https://synbiohub.org/public/igem/BBa_K1031913/annotation2360171/range2360171/1
type: Range
persistent identity: range2360171
display id: range2360171
version: 1
direction: direction/forward
top level: BBa_K1031913
start: 1034
end: 1162
orientation: inline

XylS
type: SequenceAnnotation
persistent identity: annotation2360170
display id: annotation2360170
version: 1
top level: BBa_K1031913
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1031913/annotation2360170/range2360170/1

https://synbiohub.org/public/igem/BBa_K1031913/annotation2360170/range2360170/1
type: Range
persistent identity: range2360170
display id: range2360170
version: 1
direction: direction/forward
top level: BBa_K1031913
start: 36
end: 1027
orientation: inline

BBa_K1031621
type: ComponentDefinition
persistent identity: BBa K1031621
display id: BBa_K1031621
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: J23105-NahF-Terminator
creator: He Shuaixin
created: 2013-09-08T11:00:00Z
modified: 2015-05-08T01:08:47Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1338_
m user id: 0
owner id: 15843
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: 105-NahF-TT
mutable notes: molecular cloning
mutable provenance: synthesis Pseudomonas
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: NahF
sequence annotation: J23105
sequence annotation: B0015
sequence: https://synbiohub.org/public/igem/BBa_K1031621_sequence/1

NahF
type: SequenceAnnotation
persistent identity: annotation2361695
display id: annotation2361695
version: 1
top level: BBa_K1031621
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1031621/annotation2361695/range2361695/1

https://synbiohub.org/public/igem/BBa_K1031621/annotation2361695/range2361695/1
type: Range
persistent identity: range2361695
display id: range2361695
version: 1
direction: direction/forward
top level: BBa_K1031621
start: 62
end: 1513
orientation: inline

J23105
type: SequenceAnnotation
persistent identity: annotation2361694
display id: annotation2361694
version: 1
top level: BBa_K1031621
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1031621/annotation2361694/range2361694/1

https://synbiohub.org/public/igem/BBa_K1031621/annotation2361694/range2361694/1
type: Range
persistent identity: range2361694
display id: range2361694
version: 1
direction: direction/forward
top level: BBa_K1031621
start: 1
end: 35
orientation: inline

B0015
type: SequenceAnnotation
persistent identity: annotation2361696
display id: annotation2361696
version: 1
top level: BBa_K1031621
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1031621/annotation2361696/range2361696/1

https://synbiohub.org/public/igem/BBa_K1031621/annotation2361696/range2361696/1
type: Range
persistent identity: range2361696
display id: range2361696
version: 1
direction: direction/forward
top level: BBa_K1031621
start: 1520
end: 1648
orientation: inline

BBa_K1031113
type: ComponentDefinition
persistent identity: BBa K1031113
display id: BBa_K1031113
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Po-B0031-sfGFP-Terminator (CapR)
creator: He Shuaixin
created: 2013-09-08T11:00:00Z
modified: 2015-05-08T01:08:47Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1338_
m user id: 0
owner id: 15843
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Po-31-sfGFP-TT (CapR)
mutable notes: molecular cloning
mutable provenance: synthesis
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: B0031
sequence annotation: B0015
sequence annotation: sfGFP
sequence annotation: Po (CapR)
sequence: https://synbiohub.org/public/igem/BBa_K1031113_sequence/1

B0031
type: SequenceAnnotation
persistent identity: annotation2362065
display id: annotation2362065
version: 1
top level: BBa_K1031113
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1031113/annotation2362065/range2362065/1

https://synbiohub.org/public/igem/BBa_K1031113/annotation2362065/range2362065/1
type: Range
persistent identity: range2362065
display id: range2362065
version: 1
direction: direction/forward
top level: BBa_K1031113
start: 177
end: 189
orientation: inline

B0015
type: SequenceAnnotation
persistent identity: annotation2362067
display id: annotation2362067
version: 1
top level: BBa_K1031113
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1031113/annotation2362067/range2362067/1

https://synbiohub.org/public/igem/BBa_K1031113/annotation2362067/range2362067/1
type: Range
persistent identity: range2362067
display id: range2362067
version: 1
direction: direction/forward
top level: BBa_K1031113
start: 925
end: 1054
orientation: inline

sfGFP
type: SequenceAnnotation
persistent identity: annotation2362066
display id: annotation2362066
version: 1
top level: BBa_K1031113
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1031113/annotation2362066/range2362066/1

https://synbiohub.org/public/igem/BBa_K1031113/annotation2362066/range2362066/1
type: Range
persistent identity: range2362066
display id: range2362066
version: 1
direction: direction/forward
top level: BBa_K1031113
start: 197
end: 919
orientation: inline

Po (CapR)
type: SequenceAnnotation
persistent identity: annotation2362064
display id: annotation2362064
version: 1
top level: BBa_K1031113
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1031113/annotation2362064/range2362064/1

https://synbiohub.org/public/igem/BBa_K1031113/annotation2362064/range2362064/1
type: Range
persistent identity: range2362064
display id: range2362064
version: 1
direction: direction/forward
top level: BBa_K1031113
start: 1
end: 170
orientation: inline

BBa_K1031931
type: ComponentDefinition
persistent identity: BBa K1031931
display id: BBa_K1031931
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: J23114-XylC-Terminator
creator: He Shuaixin
created: 2013-10-13T11:00:00Z
modified: 2015-05-08T01:08:48Z
discontinued: false
dominant: false
group u list: _1338_
m user id: 0
owner id: 15843
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: 114-XylC-TT
mutable notes: PCR & molecular cloning
mutable provenance: Pseudomonas.putida pWW0
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: XylC
sequence annotation: B0015
sequence annotation: J23114
sequence: https://synbiohub.org/public/igem/BBa_K1031931_sequence/1

XylC
type: SequenceAnnotation
persistent identity: annotation2369604
display id: annotation2369604
version: 1
top level: BBa_K1031931
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1031931/annotation2369604/range2369604/1

https://synbiohub.org/public/igem/BBa_K1031931/annotation2369604/range2369604/1
type: Range
persistent identity: range2369604
display id: range2369604
version: 1
direction: direction/forward
top level: BBa_K1031931
start: 54
end: 1517
orientation: inline

B0015
type: SequenceAnnotation
persistent identity: annotation2369605
display id: annotation2369605
version: 1
top level: BBa_K1031931
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1031931/annotation2369605/range2369605/1

https://synbiohub.org/public/igem/BBa_K1031931/annotation2369605/range2369605/1
type: Range
persistent identity: range2369605
display id: range2369605
version: 1
direction: direction/forward
top level: BBa_K1031931
start: 1518
end: 1646
orientation: inline

J23114
type: SequenceAnnotation
persistent identity: annotation2369603
display id: annotation2369603
version: 1
top level: BBa_K1031931
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1031931/annotation2369603/range2369603/1

https://synbiohub.org/public/igem/BBa_K1031931/annotation2369603/range2369603/1
type: Range
persistent identity: range2369603
display id: range2369603
version: 1
direction: direction/forward
top level: BBa_K1031931
start: 1
end: 35
orientation: inline

BBa_K1031420
type: ComponentDefinition
persistent identity: BBa K1031420
display id: BBa_K1031420
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ph-B0031-sfGFP-Terminator (HcaR)
creator: He Shuaixin
created: 2013-09-08T11:00:00Z
modified: 2015-05-08T01:08:47Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1338_
m user id: 0
owner id: 15843
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Ph-sfGFP-TT (HcaR)
mutable notes: molecular cloning
mutable provenance: synthesis, lab made
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: B0015
sequence annotation: sfGFP
sequence annotation: Ph (HcaR)
sequence annotation: B0031
sequence: https://synbiohub.org/public/igem/BBa_K1031420_sequence/1

B0015
type: SequenceAnnotation
persistent identity: annotation2362327
display id: annotation2362327
version: 1
top level: BBa_K1031420
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1031420/annotation2362327/range2362327/1

https://synbiohub.org/public/igem/BBa_K1031420/annotation2362327/range2362327/1
type: Range
persistent identity: range2362327
display id: range2362327
version: 1
direction: direction/forward
top level: BBa_K1031420
start: 830
end: 958
orientation: inline

sfGFP
type: SequenceAnnotation
persistent identity: annotation2362326
display id: annotation2362326
version: 1
top level: BBa_K1031420
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1031420/annotation2362326/range2362326/1

https://synbiohub.org/public/igem/BBa_K1031420/annotation2362326/range2362326/1
type: Range
persistent identity: range2362326
display id: range2362326
version: 1
direction: direction/forward
top level: BBa_K1031420
start: 101
end: 823
orientation: inline

Ph (HcaR)
type: SequenceAnnotation
persistent identity: annotation2362324
display id: annotation2362324
version: 1
top level: BBa_K1031420
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1031420/annotation2362324/range2362324/1

https://synbiohub.org/public/igem/BBa_K1031420/annotation2362324/range2362324/1
type: Range
persistent identity: range2362324
display id: range2362324
version: 1
direction: direction/forward
top level: BBa_K1031420
start: 1
end: 74
orientation: inline

B0031
type: SequenceAnnotation
persistent identity: annotation2362325
display id: annotation2362325
version: 1
top level: BBa_K1031420
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1031420/annotation2362325/range2362325/1

https://synbiohub.org/public/igem/BBa_K1031420/annotation2362325/range2362325/1
type: Range
persistent identity: range2362325
display id: range2362325
version: 1
direction: direction/forward
top level: BBa_K1031420
start: 81
end: 94
orientation: inline

BBa_B0025
type: ComponentDefinition
persistent identity: BBa B0025
display id: BBa_B0025
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: double terminator (B0015), reversed
creator: Caitlin Conboy
created: 2003-12-02T12:00:00Z
modified: 2015-08-31T04:07:20Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: B0010
sequence annotation: B0012
sequence: https://synbiohub.org/public/igem/BBa_B0025_sequence/1

B0010
type: SequenceAnnotation
persistent identity: annotation369703
display id: annotation369703
version: 1
top level: BBa_B0025
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_B0025/annotation369703/range369703/1

https://synbiohub.org/public/igem/BBa_B0025/annotation369703/range369703/1
type: Range
persistent identity: range369703
display id: range369703
version: 1
direction: direction/reverse
top level: BBa_B0025
start: 50
end: 129
orientation: inline

B0012
type: SequenceAnnotation
persistent identity: annotation369702
display id: annotation369702
version: 1
top level: BBa_B0025
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_B0025/annotation369702/range369702/1

https://synbiohub.org/public/igem/BBa_B0025/annotation369702/range369702/1
type: Range
persistent identity: range369702
display id: range369702
version: 1
direction: direction/reverse
top level: BBa_B0025
start: 1
end: 41
orientation: inline

BBa_K1031620
type: ComponentDefinition
persistent identity: BBa K1031620
display id: BBa_K1031620
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: NahF-Terminator
creator: He Shuaixin
created: 2013-09-08T11:00:00Z
modified: 2015-05-08T01:08:47Z
discontinued: false
dominant: false
group u list: _1338_
m user id: 0
owner id: 15843
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: NahF-TT
mutable notes: molecular cloning
mutable provenance: synthesis Pseudomonas
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: NahF
sequence annotation: B0015
sequence: https://synbiohub.org/public/igem/BBa_K1031620_sequence/1

NahF
type: SequenceAnnotation
persistent identity: annotation2360134
display id: annotation2360134
version: 1
top level: BBa_K1031620
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1031620/annotation2360134/range2360134/1

https://synbiohub.org/public/igem/BBa_K1031620/annotation2360134/range2360134/1
type: Range
persistent identity: range2360134
display id: range2360134
version: 1
direction: direction/forward
top level: BBa_K1031620
start: 1
end: 1452
orientation: inline

B0015
type: SequenceAnnotation
persistent identity: annotation2360136
display id: annotation2360136
version: 1
top level: BBa_K1031620
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1031620/annotation2360136/range2360136/1

https://synbiohub.org/public/igem/BBa_K1031620/annotation2360136/range2360136/1
type: Range
persistent identity: range2360136
display id: range2360136
version: 1
direction: direction/forward
top level: BBa_K1031620
start: 1459
end: 1587
orientation: inline

BBa_K1486001
type: ComponentDefinition
persistent identity: BBa K1486001
display id: BBa_K1486001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Arabinose promoter + CpxR
creator: iGem EPFL 2014
created: 2014-08-11T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: The part contains CpxR with an upstream arabinose-inducible promoter.
mutable notes: The arabinose had to be upstream of the CpxR. Also, the sequence had to be tested because of a mutation, and it works perfectly.
mutable provenance: The CpxR comes from the genome of E coli, and the arabinose promoter comes from a biobrick.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_I0500
component: BBa_K1486000
component: BBa_B0034
sequence annotation: BBa_I0500
sequence annotation: BBa_K1486000
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K1486001_sequence/1

BBa_I0500
type: Component
persistent identity: component2382072
display id: component2382072
version: 1
top level: BBa_K1486001
access: public
definition: https://synbiohub.org/public/igem/BBa_I0500/1

BBa_K1486000
type: Component
persistent identity: component2382078
display id: component2382078
version: 1
top level: BBa_K1486001
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486000/1

BBa_B0034
type: Component
persistent identity: component2382074
display id: component2382074
version: 1
top level: BBa_K1486001
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_I0500
type: SequenceAnnotation
persistent identity: annotation2382072
display id: annotation2382072
version: 1
top level: BBa_K1486001
location: https://synbiohub.org/public/igem/BBa_K1486001/annotation2382072/range2382072/1
component: BBa_I0500

https://synbiohub.org/public/igem/BBa_K1486001/annotation2382072/range2382072/1
type: Range
persistent identity: range2382072
display id: range2382072
version: 1
direction: direction/reverse
top level: BBa_K1486001
start: 1
end: 1210
orientation: inline

BBa_K1486000
type: SequenceAnnotation
persistent identity: annotation2382078
display id: annotation2382078
version: 1
top level: BBa_K1486001
location: https://synbiohub.org/public/igem/BBa_K1486001/annotation2382078/range2382078/1
component: BBa_K1486000

https://synbiohub.org/public/igem/BBa_K1486001/annotation2382078/range2382078/1
type: Range
persistent identity: range2382078
display id: range2382078
version: 1
direction: direction/reverse
top level: BBa_K1486001
start: 1223
end: 1924
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2382074
display id: annotation2382074
version: 1
top level: BBa_K1486001
location: https://synbiohub.org/public/igem/BBa_K1486001/annotation2382074/range2382074/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1486001/annotation2382074/range2382074/1
type: Range
persistent identity: range2382074
display id: range2382074
version: 1
direction: direction/reverse
top level: BBa_K1486001
start: 1211
end: 1222
orientation: inline

BBa_K1031422
type: ComponentDefinition
persistent identity: BBa K1031422
display id: BBa_K1031422
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ph(HcaR)-B0034-sfGFP
creator: He Shuaixin
created: 2013-10-17T11:00:00Z
modified: 2015-05-08T01:08:47Z
discontinued: false
dominant: false
group u list: _1338_
m user id: 0
owner id: 15843
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Ph(HcaR)-B0034-sfGFP
mutable notes: molecular cloning
mutable provenance: E.coli
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: sfGFP
sequence annotation: B0015
sequence annotation: Ph (HcaR)
sequence annotation: B0034
sequence: https://synbiohub.org/public/igem/BBa_K1031422_sequence/1

sfGFP
type: SequenceAnnotation
persistent identity: annotation2369614
display id: annotation2369614
version: 1
top level: BBa_K1031422
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1031422/annotation2369614/range2369614/1

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type: Range
persistent identity: range2369614
display id: range2369614
version: 1
direction: direction/forward
top level: BBa_K1031422
start: 101
end: 823
orientation: inline

B0015
type: SequenceAnnotation
persistent identity: annotation2369616
display id: annotation2369616
version: 1
top level: BBa_K1031422
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1031422/annotation2369616/range2369616/1

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type: Range
persistent identity: range2369616
display id: range2369616
version: 1
direction: direction/forward
top level: BBa_K1031422
start: 830
end: 958
orientation: inline

Ph (HcaR)
type: SequenceAnnotation
persistent identity: annotation2369613
display id: annotation2369613
version: 1
top level: BBa_K1031422
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1031422/annotation2369613/range2369613/1

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type: Range
persistent identity: range2369613
display id: range2369613
version: 1
direction: direction/forward
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start: 1
end: 74
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation2369615
display id: annotation2369615
version: 1
top level: BBa_K1031422
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1031422/annotation2369615/range2369615/1

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type: Range
persistent identity: range2369615
display id: range2369615
version: 1
direction: direction/forward
top level: BBa_K1031422
start: 84
end: 95
orientation: inline

BBa_K1486009
type: ComponentDefinition
persistent identity: BBa K1486009
display id: BBa_K1486009
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CxpR & Split IFP1.4 [Nterm + Nterm]
creator: iGem EPFL 2014
created: 2014-08-26T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part has two fused proteins downstream of an arabinose-inducible promoter: CpxR-IFP[1] and CpxR-IFP[2]. This construct was made in all orientations: - CpxR-IFP[1] + CpxR-IFP[2] (BBa_K1286008) - IFP[1]-CpxR + CpxR-IFP[2] (BBa_K1486010) - CpxR-IFP[1] + IFP[2]-CpxR (BBa_K1486011)
mutable notes: All orientations were made, so all dimerizations could be tested (see main page for biobrick numbers). Flexible linkers were used for each of the fused proteins, and an RBS was put before each one of them.
mutable provenance: From Michnick Article. To Edit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K1486000
component: BBa_K1486006
component: BBa_I0500
component: BBa_K1486007
component: BBa_K1486004
component: BBa_B0034
component: BBa_K1486052
sequence annotation: BBa_B0034
sequence annotation: BBa_K1486004
sequence annotation: BBa_I0500
sequence annotation: BBa_K1486006
sequence annotation: BBa_K1486000
sequence annotation: BBa_K1486052
sequence annotation: BBa_K1486007
sequence: https://synbiohub.org/public/igem/BBa_K1486009_sequence/1

BBa_K1486000
type: Component
persistent identity: component2420788
display id: component2420788
version: 1
top level: BBa_K1486009
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486000/1

BBa_K1486006
type: Component
persistent identity: component2420771
display id: component2420771
version: 1
top level: BBa_K1486009
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486006/1

BBa_I0500
type: Component
persistent identity: component2420763
display id: component2420763
version: 1
top level: BBa_K1486009
access: public
definition: https://synbiohub.org/public/igem/BBa_I0500/1

BBa_K1486007
type: Component
persistent identity: component2420783
display id: component2420783
version: 1
top level: BBa_K1486009
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486007/1

BBa_K1486000
type: Component
persistent identity: component2420776
display id: component2420776
version: 1
top level: BBa_K1486009
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486000/1

BBa_K1486004
type: Component
persistent identity: component2420784
display id: component2420784
version: 1
top level: BBa_K1486009
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486004/1

BBa_K1486004
type: Component
persistent identity: component2420772
display id: component2420772
version: 1
top level: BBa_K1486009
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486004/1

BBa_K1486004
type: Component
persistent identity: component2420758
display id: component2420758
version: 1
top level: BBa_K1486009
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486004/1

BBa_K1486000
type: Component
persistent identity: component2420766
display id: component2420766
version: 1
top level: BBa_K1486009
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486000/1

BBa_B0034
type: Component
persistent identity: component2420779
display id: component2420779
version: 1
top level: BBa_K1486009
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K1486052
type: Component
persistent identity: component2420777
display id: component2420777
version: 1
top level: BBa_K1486009
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486052/1

BBa_K1486007
type: Component
persistent identity: component2420756
display id: component2420756
version: 1
top level: BBa_K1486009
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486007/1

BBa_B0034
type: Component
persistent identity: component2420767
display id: component2420767
version: 1
top level: BBa_K1486009
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0034
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display id: annotation2420779
version: 1
top level: BBa_K1486009
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component: BBa_B0034

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display id: range2420779
version: 1
direction: direction/reverse
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start: 2398
end: 2409
orientation: inline

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type: SequenceAnnotation
persistent identity: annotation2420758
display id: annotation2420758
version: 1
top level: BBa_K1486009
location: https://synbiohub.org/public/igem/BBa_K1486009/annotation2420758/range2420758/1
component: BBa_K1486004

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persistent identity: range2420758
display id: range2420758
version: 1
direction: direction/reverse
top level: BBa_K1486009
start: 2995
end: 3024
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BBa_K1486004
type: SequenceAnnotation
persistent identity: annotation2420772
display id: annotation2420772
version: 1
top level: BBa_K1486009
location: https://synbiohub.org/public/igem/BBa_K1486009/annotation2420772/range2420772/1
component: BBa_K1486004

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end: 1657
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BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2420767
display id: annotation2420767
version: 1
top level: BBa_K1486009
location: https://synbiohub.org/public/igem/BBa_K1486009/annotation2420767/range2420767/1
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persistent identity: range2420767
display id: range2420767
version: 1
direction: direction/reverse
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persistent identity: annotation2420763
display id: annotation2420763
version: 1
top level: BBa_K1486009
location: https://synbiohub.org/public/igem/BBa_K1486009/annotation2420763/range2420763/1
component: BBa_I0500

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version: 1
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persistent identity: annotation2420776
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version: 1
top level: BBa_K1486009
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BBa_K1486052
type: SequenceAnnotation
persistent identity: annotation2420777
display id: annotation2420777
version: 1
top level: BBa_K1486009
location: https://synbiohub.org/public/igem/BBa_K1486009/annotation2420777/range2420777/1
component: BBa_K1486052

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persistent identity: range2420777
display id: range2420777
version: 1
direction: direction/reverse
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start: 2360
end: 2397
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BBa_K1486007
type: SequenceAnnotation
persistent identity: annotation2420783
display id: annotation2420783
version: 1
top level: BBa_K1486009
location: https://synbiohub.org/public/igem/BBa_K1486009/annotation2420783/range2420783/1
component: BBa_K1486007

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persistent identity: range2420783
display id: range2420783
version: 1
direction: direction/reverse
top level: BBa_K1486009
start: 2410
end: 2994
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BBa_K1486000
type: SequenceAnnotation
persistent identity: annotation2420788
display id: annotation2420788
version: 1
top level: BBa_K1486009
location: https://synbiohub.org/public/igem/BBa_K1486009/annotation2420788/range2420788/1
component: BBa_K1486000

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type: Range
persistent identity: range2420788
display id: range2420788
version: 1
direction: direction/reverse
top level: BBa_K1486009
start: 3025
end: 3726
orientation: inline

BBa_K1486004
type: SequenceAnnotation
persistent identity: annotation2420784
display id: annotation2420784
version: 1
top level: BBa_K1486009
location: https://synbiohub.org/public/igem/BBa_K1486009/annotation2420784/range2420784/1
component: BBa_K1486004

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type: Range
persistent identity: range2420784
display id: range2420784
version: 1
direction: direction/reverse
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start: 2995
end: 3024
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BBa_K1486007
type: SequenceAnnotation
persistent identity: annotation2420756
display id: annotation2420756
version: 1
top level: BBa_K1486009
location: https://synbiohub.org/public/igem/BBa_K1486009/annotation2420756/range2420756/1
component: BBa_K1486007

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persistent identity: range2420756
display id: range2420756
version: 1
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start: 2410
end: 2994
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BBa_K1486000
type: SequenceAnnotation
persistent identity: annotation2420766
display id: annotation2420766
version: 1
top level: BBa_K1486009
location: https://synbiohub.org/public/igem/BBa_K1486009/annotation2420766/range2420766/1
component: BBa_K1486000

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type: Range
persistent identity: range2420766
display id: range2420766
version: 1
direction: direction/reverse
top level: BBa_K1486009
start: 3025
end: 3726
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BBa_K1031221
type: ComponentDefinition
persistent identity: BBa K1031221
display id: BBa_K1031221
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Po-B0031-sfGFP-Terminator (DmpR)
creator: He Shuaixin
created: 2013-09-08T11:00:00Z
modified: 2015-05-08T01:08:47Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1338_
m user id: 0
owner id: 15843
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: Po-31-sfGFP-TT (DmpR)
mutable notes: molecular cloning
mutable provenance: synthesis, lab made
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: sfGFP
sequence annotation: B0031
sequence annotation: B0015
sequence annotation: Po promoter
sequence: https://synbiohub.org/public/igem/BBa_K1031221_sequence/1

sfGFP
type: SequenceAnnotation
persistent identity: annotation2354352
display id: annotation2354352
version: 1
top level: BBa_K1031221
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1031221/annotation2354352/range2354352/1

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type: Range
persistent identity: range2354352
display id: range2354352
version: 1
direction: direction/forward
top level: BBa_K1031221
start: 200
end: 922
orientation: inline

B0031
type: SequenceAnnotation
persistent identity: annotation2354351
display id: annotation2354351
version: 1
top level: BBa_K1031221
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1031221/annotation2354351/range2354351/1

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type: Range
persistent identity: range2354351
display id: range2354351
version: 1
direction: direction/forward
top level: BBa_K1031221
start: 180
end: 193
orientation: inline

B0015
type: SequenceAnnotation
persistent identity: annotation2354353
display id: annotation2354353
version: 1
top level: BBa_K1031221
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1031221/annotation2354353/range2354353/1

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type: Range
persistent identity: range2354353
display id: range2354353
version: 1
direction: direction/forward
top level: BBa_K1031221
start: 931
end: 1059
orientation: inline

Po promoter
type: SequenceAnnotation
persistent identity: annotation2354350
display id: annotation2354350
version: 1
top level: BBa_K1031221
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1031221/annotation2354350/range2354350/1

https://synbiohub.org/public/igem/BBa_K1031221/annotation2354350/range2354350/1
type: Range
persistent identity: range2354350
display id: range2354350
version: 1
direction: direction/forward
top level: BBa_K1031221
start: 1
end: 171
orientation: inline

BBa_K1486005
type: ComponentDefinition
persistent identity: BBa K1486005
display id: BBa_K1486005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Arabinose promoter + sfGFP-CpxR [Cterm]
creator: iGem EPFL 2014
created: 2014-08-12T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part is composed of an arabinose-inducible promoter for superfolded GFP attached by a linker to CpxR (on the C terminus of CpxR).
mutable notes: We had to test both attachments, N and C terminus of the CpxR.
mutable provenance: The arabinose promoter and the sfGFP were obtained from the registry, while the CpxR was obtained from the E coli genome.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K1486003
component: BBa_I746916
component: BBa_B0034
component: BBa_K1486000
component: BBa_I0500
sequence annotation: BBa_I746916
sequence annotation: BBa_I0500
sequence annotation: BBa_K1486000
sequence annotation: BBa_B0034
sequence annotation: BBa_K1486003
sequence: https://synbiohub.org/public/igem/BBa_K1486005_sequence/1

BBa_K1486003
type: Component
persistent identity: component2382089
display id: component2382089
version: 1
top level: BBa_K1486005
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486003/1

BBa_I746916
type: Component
persistent identity: component2382093
display id: component2382093
version: 1
top level: BBa_K1486005
access: public
definition: https://synbiohub.org/public/igem/BBa_I746916/1

BBa_B0034
type: Component
persistent identity: component2382084
display id: component2382084
version: 1
top level: BBa_K1486005
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

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persistent identity: component2382088
display id: component2382088
version: 1
top level: BBa_K1486005
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486000/1

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persistent identity: component2382082
display id: component2382082
version: 1
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access: public
definition: https://synbiohub.org/public/igem/BBa_I0500/1

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display id: annotation2382093
version: 1
top level: BBa_K1486005
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version: 1
direction: direction/reverse
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display id: annotation2382082
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top level: BBa_K1486005
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direction: direction/reverse
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persistent identity: annotation2382084
display id: annotation2382084
version: 1
top level: BBa_K1486005
location: https://synbiohub.org/public/igem/BBa_K1486005/annotation2382084/range2382084/1
component: BBa_B0034

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persistent identity: range2382084
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version: 1
direction: direction/reverse
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start: 1211
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persistent identity: annotation2382089
display id: annotation2382089
version: 1
top level: BBa_K1486005
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version: 1
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type: ComponentDefinition
persistent identity: BBa K1031311
display id: BBa_K1031311
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: J23114-HbpR-Terminator
creator: He Shuaixin
created: 2013-10-12T11:00:00Z
modified: 2015-05-08T01:08:47Z
discontinued: false
dominant: false
group u list: _1338_
m user id: 0
owner id: 15843
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: 114-HbpR-TT
mutable notes: molecular cloning
mutable provenance: P.putida
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: J23114
sequence annotation: HbpR
sequence annotation: B0015
sequence: https://synbiohub.org/public/igem/BBa_K1031311_sequence/1

J23114
type: SequenceAnnotation
persistent identity: annotation2369597
display id: annotation2369597
version: 1
top level: BBa_K1031311
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1031311/annotation2369597/range2369597/1

https://synbiohub.org/public/igem/BBa_K1031311/annotation2369597/range2369597/1
type: Range
persistent identity: range2369597
display id: range2369597
version: 1
direction: direction/forward
top level: BBa_K1031311
start: 1
end: 35
orientation: inline

HbpR
type: SequenceAnnotation
persistent identity: annotation2369598
display id: annotation2369598
version: 1
top level: BBa_K1031311
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1031311/annotation2369598/range2369598/1

https://synbiohub.org/public/igem/BBa_K1031311/annotation2369598/range2369598/1
type: Range
persistent identity: range2369598
display id: range2369598
version: 1
direction: direction/forward
top level: BBa_K1031311
start: 63
end: 1713
orientation: inline

B0015
type: SequenceAnnotation
persistent identity: annotation2369599
display id: annotation2369599
version: 1
top level: BBa_K1031311
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1031311/annotation2369599/range2369599/1

https://synbiohub.org/public/igem/BBa_K1031311/annotation2369599/range2369599/1
type: Range
persistent identity: range2369599
display id: range2369599
version: 1
direction: direction/forward
top level: BBa_K1031311
start: 1782
end: 1910
orientation: inline

BBa_K1031521
type: ComponentDefinition
persistent identity: BBa K1031521
display id: BBa_K1031521
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Pg-B0032-sfGFP-Terminator (HpaR)
creator: He Shuaixin
created: 2013-09-08T11:00:00Z
modified: 2015-05-08T01:08:47Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1338_
m user id: 0
owner id: 15843
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Pg-32-sfGFP-TT (HpaR)
mutable notes: molecular cloning
mutable provenance: synthesis, lab made
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: B0032
sequence annotation: B0015
sequence annotation: Pg (HpaR)
sequence annotation: sfGFP
sequence: https://synbiohub.org/public/igem/BBa_K1031521_sequence/1

B0032
type: SequenceAnnotation
persistent identity: annotation2362522
display id: annotation2362522
version: 1
top level: BBa_K1031521
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1031521/annotation2362522/range2362522/1

https://synbiohub.org/public/igem/BBa_K1031521/annotation2362522/range2362522/1
type: Range
persistent identity: range2362522
display id: range2362522
version: 1
direction: direction/forward
top level: BBa_K1031521
start: 234
end: 246
orientation: inline

B0015
type: SequenceAnnotation
persistent identity: annotation2362524
display id: annotation2362524
version: 1
top level: BBa_K1031521
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1031521/annotation2362524/range2362524/1

https://synbiohub.org/public/igem/BBa_K1031521/annotation2362524/range2362524/1
type: Range
persistent identity: range2362524
display id: range2362524
version: 1
direction: direction/forward
top level: BBa_K1031521
start: 982
end: 1110
orientation: inline

Pg (HpaR)
type: SequenceAnnotation
persistent identity: annotation2362521
display id: annotation2362521
version: 1
top level: BBa_K1031521
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1031521/annotation2362521/range2362521/1

https://synbiohub.org/public/igem/BBa_K1031521/annotation2362521/range2362521/1
type: Range
persistent identity: range2362521
display id: range2362521
version: 1
direction: direction/forward
top level: BBa_K1031521
start: 1
end: 227
orientation: inline

sfGFP
type: SequenceAnnotation
persistent identity: annotation2362523
display id: annotation2362523
version: 1
top level: BBa_K1031521
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1031521/annotation2362523/range2362523/1

https://synbiohub.org/public/igem/BBa_K1031521/annotation2362523/range2362523/1
type: Range
persistent identity: range2362523
display id: range2362523
version: 1
direction: direction/forward
top level: BBa_K1031521
start: 253
end: 975
orientation: inline

BBa_K1031622
type: ComponentDefinition
persistent identity: BBa K1031622
display id: BBa_K1031622
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: J23106-NahF-Terminator
creator: He Shuaixin
created: 2013-09-08T11:00:00Z
modified: 2015-05-08T01:08:47Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1338_
m user id: 0
owner id: 15843
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: 106-NahF-TT
mutable notes: molecular cloning
mutable provenance: synthesis Pseudomonas
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: B0015
sequence annotation: NahF
sequence annotation: J23106
sequence: https://synbiohub.org/public/igem/BBa_K1031622_sequence/1

B0015
type: SequenceAnnotation
persistent identity: annotation2361881
display id: annotation2361881
version: 1
top level: BBa_K1031622
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1031622/annotation2361881/range2361881/1

https://synbiohub.org/public/igem/BBa_K1031622/annotation2361881/range2361881/1
type: Range
persistent identity: range2361881
display id: range2361881
version: 1
direction: direction/forward
top level: BBa_K1031622
start: 1519
end: 1648
orientation: inline

NahF
type: SequenceAnnotation
persistent identity: annotation2361879
display id: annotation2361879
version: 1
top level: BBa_K1031622
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1031622/annotation2361879/range2361879/1

https://synbiohub.org/public/igem/BBa_K1031622/annotation2361879/range2361879/1
type: Range
persistent identity: range2361879
display id: range2361879
version: 1
direction: direction/forward
top level: BBa_K1031622
start: 62
end: 1513
orientation: inline

J23106
type: SequenceAnnotation
persistent identity: annotation2361878
display id: annotation2361878
version: 1
top level: BBa_K1031622
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1031622/annotation2361878/range2361878/1

https://synbiohub.org/public/igem/BBa_K1031622/annotation2361878/range2361878/1
type: Range
persistent identity: range2361878
display id: range2361878
version: 1
direction: direction/forward
top level: BBa_K1031622
start: 1
end: 35
orientation: inline

BBa_B0015
type: ComponentDefinition
persistent identity: BBa B0015
display id: BBa_B0015
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: double terminator (B0010-B0012)
creator: Reshma Shetty
created: 2003-07-16T11:00:00Z
modified: 2015-08-31T04:07:20Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Double terminator consisting of BBa_B0010 and BBa_B0012
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000141
role: partType/Terminator
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_B0010
sequence: https://synbiohub.org/public/igem/BBa_B0015_sequence/1

BBa_B0012
type: Component
persistent identity: component1916612
display id: component1916612
version: 1
top level: BBa_B0015
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0010
type: Component
persistent identity: component1916610
display id: component1916610
version: 1
top level: BBa_B0015
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1916612
display id: annotation1916612
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
type: Range
persistent identity: range1916612
display id: range1916612
version: 1
direction: direction/forward
top level: BBa_B0015
start: 89
end: 129
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1916610
display id: annotation1916610
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
type: Range
persistent identity: range1916610
display id: range1916610
version: 1
direction: direction/forward
top level: BBa_B0015
start: 1
end: 80
orientation: inline

BBa_B0017
type: ComponentDefinition
persistent identity: BBa B0017
display id: BBa_B0017
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: double terminator (B0010-B0010)
creator: Austin Che
created: 2004-01-08T12:00:00Z
modified: 2015-08-31T04:07:20Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Double terminator with two copies of <bb_part>BBa_B0010</bb_part>
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000141
role: partType/Terminator
component: BBa_B0010
sequence annotation: BBa_B0010
sequence: https://synbiohub.org/public/igem/BBa_B0017_sequence/1

BBa_B0010
type: Component
persistent identity: component939331
display id: component939331
version: 1
top level: BBa_B0017
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0010
type: Component
persistent identity: component939337
display id: component939337
version: 1
top level: BBa_B0017
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation939331
display id: annotation939331
version: 1
top level: BBa_B0017
location: https://synbiohub.org/public/igem/BBa_B0017/annotation939331/range939331/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_B0017/annotation939331/range939331/1
type: Range
persistent identity: range939331
display id: range939331
version: 1
direction: direction/forward
top level: BBa_B0017
start: 1
end: 80
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation939337
display id: annotation939337
version: 1
top level: BBa_B0017
location: https://synbiohub.org/public/igem/BBa_B0017/annotation939337/range939337/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_B0017/annotation939337/range939337/1
type: Range
persistent identity: range939337
display id: range939337
version: 1
direction: direction/forward
top level: BBa_B0017
start: 89
end: 168
orientation: inline

BBa_K259006
type: ComponentDefinition
persistent identity: BBa K259006
display id: BBa_K259006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: GFP-Terminator
creator: Petros Mina
created: 2009-09-08T11:00:00Z
modified: 2015-05-08T01:11:42Z
discontinued: false
dominant: false
group u list: _357_
m user id: 0
owner id: 5318
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: ==Part Description== This is a composite part made from Green Fluorescent Protein (GFP) and a double terminator. Green Fluorescent protein (originally from part [http://partsregistry.org/Part:BBa_E0040 BBa_E0040]) that will glow green when excited by the correct wavelengths and thus can be used as a reporter gene. This part is directly fused to a terminator (originally from part [http://partsregistry.org/Part:BBa_B0014 BBa_B0014]) so it is ideal to be fused at the end of your construct. ==Table of Statistics== {| style="color:white; background-color:#0066CC;" cellpadding="20" cellspacing="0" border="2" !Chassis !Monomer/Multimer !Aminoacid Length/Weight !Localisation !Structure !Excitation wavelengths !Emmision wavelengths |- |Prokaryotic and Eukaryotic |Bipartite |274 aminoacids | |beta-barrel(for GFP) hairpins for Terminator |501nm |511nm |} ==References== <biblio> #Valdivia pmid=8707053 </biblio>
mutable notes: No additional design considerations. The parts were fused together following RFC10 assembly.
mutable provenance: GFP originates from jellyfish Aequeora victoria. For more details visit the [http://www.expasy.ch/uniprot/P42212 SwissProt entry]. The double terminator was designed by Reshma Shetty. For more details visis the [http://partsregistry.org/Part:BBa_B0014 original part page].
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_B0012
component: BBa_E0040
component: BBa_B0011
sequence annotation: BBa_B0011
sequence annotation: BBa_B0012
sequence annotation: BBa_E0040
sequence: https://synbiohub.org/public/igem/BBa_K259006_sequence/1

BBa_B0012
type: Component
persistent identity: component2020530
display id: component2020530
version: 1
top level: BBa_K259006
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_E0040
type: Component
persistent identity: component2020529
display id: component2020529
version: 1
top level: BBa_K259006
access: public
definition: https://synbiohub.org/public/igem/BBa_E0040/1

BBa_B0011
type: Component
persistent identity: component2020534
display id: component2020534
version: 1
top level: BBa_K259006
access: public
definition: https://synbiohub.org/public/igem/BBa_B0011/1

BBa_B0011
type: SequenceAnnotation
persistent identity: annotation2020534
display id: annotation2020534
version: 1
top level: BBa_K259006
location: https://synbiohub.org/public/igem/BBa_K259006/annotation2020534/range2020534/1
component: BBa_B0011

https://synbiohub.org/public/igem/BBa_K259006/annotation2020534/range2020534/1
type: Range
persistent identity: range2020534
display id: range2020534
version: 1
direction: direction/forward
top level: BBa_K259006
start: 778
end: 823
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2020530
display id: annotation2020530
version: 1
top level: BBa_K259006
location: https://synbiohub.org/public/igem/BBa_K259006/annotation2020530/range2020530/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K259006/annotation2020530/range2020530/1
type: Range
persistent identity: range2020530
display id: range2020530
version: 1
direction: direction/forward
top level: BBa_K259006
start: 729
end: 769
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation2020529
display id: annotation2020529
version: 1
top level: BBa_K259006
location: https://synbiohub.org/public/igem/BBa_K259006/annotation2020529/range2020529/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_K259006/annotation2020529/range2020529/1
type: Range
persistent identity: range2020529
display id: range2020529
version: 1
direction: direction/reverse
top level: BBa_K259006
start: 1
end: 720
orientation: inline

BBa_K1031623
type: ComponentDefinition
persistent identity: BBa K1031623
display id: BBa_K1031623
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: J23113-NahF-Terminator
creator: He Shuaixin
created: 2013-09-08T11:00:00Z
modified: 2015-05-08T01:08:47Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1338_
m user id: 0
owner id: 15843
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: 113-NahF-TT
mutable notes: molecular cloning
mutable provenance: synthesis Pseudomonas
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: NahF
sequence annotation: J23113
sequence annotation: B0015
sequence: https://synbiohub.org/public/igem/BBa_K1031623_sequence/1

NahF
type: SequenceAnnotation
persistent identity: annotation2361891
display id: annotation2361891
version: 1
top level: BBa_K1031623
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1031623/annotation2361891/range2361891/1

https://synbiohub.org/public/igem/BBa_K1031623/annotation2361891/range2361891/1
type: Range
persistent identity: range2361891
display id: range2361891
version: 1
direction: direction/forward
top level: BBa_K1031623
start: 62
end: 1513
orientation: inline

J23113
type: SequenceAnnotation
persistent identity: annotation2361890
display id: annotation2361890
version: 1
top level: BBa_K1031623
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1031623/annotation2361890/range2361890/1

https://synbiohub.org/public/igem/BBa_K1031623/annotation2361890/range2361890/1
type: Range
persistent identity: range2361890
display id: range2361890
version: 1
direction: direction/forward
top level: BBa_K1031623
start: 1
end: 35
orientation: inline

B0015
type: SequenceAnnotation
persistent identity: annotation2361894
display id: annotation2361894
version: 1
top level: BBa_K1031623
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1031623/annotation2361894/range2361894/1

https://synbiohub.org/public/igem/BBa_K1031623/annotation2361894/range2361894/1
type: Range
persistent identity: range2361894
display id: range2361894
version: 1
direction: direction/forward
top level: BBa_K1031623
start: 1520
end: 1648
orientation: inline

BBa_K1031922
type: ComponentDefinition
persistent identity: BBa K1031922
display id: BBa_K1031922
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: J23114-XylB-Terminator
creator: He Shuaixin
created: 2013-10-21T11:00:00Z
modified: 2015-05-08T01:08:48Z
discontinued: false
dominant: false
group u list: _1338_
m user id: 0
owner id: 15843
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: J23114-XylB-Terminator
mutable notes: molecular cloning
mutable provenance: Pseudomonas.putida
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: B0015
sequence annotation: XylB
sequence annotation: J23114
sequence: https://synbiohub.org/public/igem/BBa_K1031922_sequence/1

B0015
type: SequenceAnnotation
persistent identity: annotation2370038
display id: annotation2370038
version: 1
top level: BBa_K1031922
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1031922/annotation2370038/range2370038/1

https://synbiohub.org/public/igem/BBa_K1031922/annotation2370038/range2370038/1
type: Range
persistent identity: range2370038
display id: range2370038
version: 1
direction: direction/forward
top level: BBa_K1031922
start: 1176
end: 1304
orientation: inline

XylB
type: SequenceAnnotation
persistent identity: annotation2370037
display id: annotation2370037
version: 1
top level: BBa_K1031922
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1031922/annotation2370037/range2370037/1

https://synbiohub.org/public/igem/BBa_K1031922/annotation2370037/range2370037/1
type: Range
persistent identity: range2370037
display id: range2370037
version: 1
direction: direction/forward
top level: BBa_K1031922
start: 63
end: 1169
orientation: inline

J23114
type: SequenceAnnotation
persistent identity: annotation2370036
display id: annotation2370036
version: 1
top level: BBa_K1031922
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1031922/annotation2370036/range2370036/1

https://synbiohub.org/public/igem/BBa_K1031922/annotation2370036/range2370036/1
type: Range
persistent identity: range2370036
display id: range2370036
version: 1
direction: direction/forward
top level: BBa_K1031922
start: 1
end: 35
orientation: inline

Reporter +
type: ComponentDefinition
persistent identity: BBa K653001
display id: BBa_K653001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: GFP reporter plus Double Terminator
creator: Ernesto G??mez
created: 2011-09-27T11:00:00Z
modified: 2015-05-08T01:13:00Z
discontinued: false
dominant: false
experience: experience/None
group u list: _830_
m user id: 0
owner id: 7119
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: The iGEM Team Panama 2011 have to design and assemble the ends of an expression platform in order to bring up by rational designing a fuctional test platform for the expression of our Team favorite BioBrick part BBa_K653000 A.K. The Biosurfactator. We choose to assembly two existing BioBrick parts from the shop, we mean The Registry. Those parts are: a GFP reporter BBa_E0040 and a double terminator BBa_B0015. We assemble them to bring up a new composite part that will serve as a well characterized ends for a expression platform for several coding sequences like our RhlAB_BBa_K653000
mutable notes: This GFP part was derived from the reporter device used by Elowitz's repressilator : GFPmut3* has a couple of mutations from wtGFP - S2R and S65G and S72A. Emerald and EGFP have S65T and multiple other mutations. The S2R mutation should have no effect. The Double terminator consisting of BBa_B0010 and BBa_B0012. This is the most commonly used terminator. It seems to be reliable. The assembling of this two parts was performed using the 3A Assembly Method.
mutable provenance: atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtg aaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatgg tgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttc aaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatg gaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaat tagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattac ctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatgg atgaactatacaaataataa ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctc accttcgggtgggcctttctgcgtttata
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_E0040
component: BBa_B0015
sequence annotation: BBa_B0015
sequence annotation: BBa_E0040
sequence: https://synbiohub.org/public/igem/BBa_K653001_sequence/1

BBa_E0040
type: Component
persistent identity: component2146079
display id: component2146079
version: 1
top level: Reporter +
access: public
definition: https://synbiohub.org/public/igem/BBa_E0040/1

BBa_B0015
type: Component
persistent identity: component2146086
display id: component2146086
version: 1
top level: Reporter +
access: public
definition: BBa_B0015

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2146086
display id: annotation2146086
version: 1
top level: Reporter +
location: https://synbiohub.org/public/igem/BBa_K653001/annotation2146086/range2146086/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K653001/annotation2146086/range2146086/1
type: Range
persistent identity: range2146086
display id: range2146086
version: 1
direction: direction/reverse
top level: Reporter +
start: 729
end: 857
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation2146079
display id: annotation2146079
version: 1
top level: Reporter +
location: https://synbiohub.org/public/igem/BBa_K653001/annotation2146079/range2146079/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_K653001/annotation2146079/range2146079/1
type: Range
persistent identity: range2146079
display id: range2146079
version: 1
direction: direction/reverse
top level: Reporter +
start: 1
end: 720
orientation: inline

BBa_K2122000
type: ComponentDefinition
persistent identity: BBa K2122000
display id: BBa_K2122000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Device expressing the Gb3 Synthase enzyme under control of an arabinose inducible promoter
creator: Sannia Farrukh
created: 2016-09-28T11:00:00Z
modified: 2016-10-18T01:26:30Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2591_
m user id: 32074
owner id: 32074
owning group id: 9
status: status/Planning
bookmark: true
mutable description: This part will code for Gb3 synthase. Gb3 synthase in return makes Gb3 which is a receptor for Shiga-like toxin (stx 1&2). Shiga-like toxins are exotoxins, which consist of a toxic enzymatic A subunit and a cell-binding B subunit. The latter binds to a globotriaosylceramide (Gb3) receptor, expressed on the surface of the target cells in humans. This interaction is responsible for the toxin's entry into the host cell. When the receptor is exposed to the food sample, the SLT subunit B, if present, will bind to Gb3.
mutable notes: An inducible promoter (BBa_10500), RBS (BBa_B0034) Scar was added to the sequence. Along with a terminator.
mutable provenance: Coding Sequence from GenBank
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_K2122150
component: BBa_B0034
component: BBa_I0500
component: BBa_B0014
sequence annotation: BBa_B0014
sequence annotation: BBa_I0500
sequence annotation: BBa_B0034
sequence annotation: BBa_K2122150
sequence: https://synbiohub.org/public/igem/BBa_K2122000_sequence/1

BBa_K2122150
type: Component
persistent identity: component2517225
display id: component2517225
version: 1
top level: BBa_K2122000
access: public
definition: https://synbiohub.org/public/igem/BBa_K2122150/1

BBa_B0034
type: Component
persistent identity: component2517224
display id: component2517224
version: 1
top level: BBa_K2122000
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_I0500
type: Component
persistent identity: component2517222
display id: component2517222
version: 1
top level: BBa_K2122000
access: public
definition: https://synbiohub.org/public/igem/BBa_I0500/1

BBa_B0014
type: Component
persistent identity: component2517232
display id: component2517232
version: 1
top level: BBa_K2122000
access: public
definition: BBa_B0014

BBa_B0014
type: SequenceAnnotation
persistent identity: annotation2517232
display id: annotation2517232
version: 1
top level: BBa_K2122000
location: https://synbiohub.org/public/igem/BBa_K2122000/annotation2517232/range2517232/1
component: BBa_B0014

https://synbiohub.org/public/igem/BBa_K2122000/annotation2517232/range2517232/1
type: Range
persistent identity: range2517232
display id: range2517232
version: 1
direction: direction/reverse
top level: BBa_K2122000
start: 2307
end: 2401
orientation: inline

BBa_I0500
type: SequenceAnnotation
persistent identity: annotation2517222
display id: annotation2517222
version: 1
top level: BBa_K2122000
location: https://synbiohub.org/public/igem/BBa_K2122000/annotation2517222/range2517222/1
component: BBa_I0500

https://synbiohub.org/public/igem/BBa_K2122000/annotation2517222/range2517222/1
type: Range
persistent identity: range2517222
display id: range2517222
version: 1
direction: direction/reverse
top level: BBa_K2122000
start: 1
end: 1210
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2517224
display id: annotation2517224
version: 1
top level: BBa_K2122000
location: https://synbiohub.org/public/igem/BBa_K2122000/annotation2517224/range2517224/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K2122000/annotation2517224/range2517224/1
type: Range
persistent identity: range2517224
display id: range2517224
version: 1
direction: direction/reverse
top level: BBa_K2122000
start: 1219
end: 1230
orientation: inline

BBa_K2122150
type: SequenceAnnotation
persistent identity: annotation2517225
display id: annotation2517225
version: 1
top level: BBa_K2122000
location: https://synbiohub.org/public/igem/BBa_K2122000/annotation2517225/range2517225/1
component: BBa_K2122150

https://synbiohub.org/public/igem/BBa_K2122000/annotation2517225/range2517225/1
type: Range
persistent identity: range2517225
display id: range2517225
version: 1
direction: direction/reverse
top level: BBa_K2122000
start: 1237
end: 2298
orientation: inline

BBa_K1031112
type: ComponentDefinition
persistent identity: BBa K1031112
display id: BBa_K1031112
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: J23113-CapR-Terminator
creator: He Shuaixin
created: 2013-09-08T11:00:00Z
modified: 2015-05-08T01:08:47Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1338_
m user id: 0
owner id: 15843
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: 113-CapR-TT
mutable notes: molecular cloning
mutable provenance: synthesis Pseudomonas putida
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: J23113
sequence annotation: B0015
sequence annotation: CapR
sequence: https://synbiohub.org/public/igem/BBa_K1031112_sequence/1

J23113
type: SequenceAnnotation
persistent identity: annotation2361448
display id: annotation2361448
version: 1
top level: BBa_K1031112
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1031112/annotation2361448/range2361448/1

https://synbiohub.org/public/igem/BBa_K1031112/annotation2361448/range2361448/1
type: Range
persistent identity: range2361448
display id: range2361448
version: 1
direction: direction/forward
top level: BBa_K1031112
start: 1
end: 35
orientation: inline

B0015
type: SequenceAnnotation
persistent identity: annotation2361508
display id: annotation2361508
version: 1
top level: BBa_K1031112
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1031112/annotation2361508/range2361508/1

https://synbiohub.org/public/igem/BBa_K1031112/annotation2361508/range2361508/1
type: Range
persistent identity: range2361508
display id: range2361508
version: 1
direction: direction/forward
top level: BBa_K1031112
start: 1760
end: 1888
orientation: inline

CapR
type: SequenceAnnotation
persistent identity: annotation2361507
display id: annotation2361507
version: 1
top level: BBa_K1031112
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1031112/annotation2361507/range2361507/1

https://synbiohub.org/public/igem/BBa_K1031112/annotation2361507/range2361507/1
type: Range
persistent identity: range2361507
display id: range2361507
version: 1
direction: direction/forward
top level: BBa_K1031112
start: 62
end: 1753
orientation: inline

BBa_K1486025
type: ComponentDefinition
persistent identity: BBa K1486025
display id: BBa_K1486025
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: ADH1 Terminator
creator: EPFL iGem team 2014
created: 2014-09-25T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: To edit
mutable notes: To edit
mutable provenance: To edit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: Adh1 terminator
sequence: https://synbiohub.org/public/igem/BBa_K1486025_sequence/1

Adh1 terminator
type: SequenceAnnotation
persistent identity: annotation2418776
display id: annotation2418776
version: 1
top level: BBa_K1486025
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1486025/annotation2418776/range2418776/1

https://synbiohub.org/public/igem/BBa_K1486025/annotation2418776/range2418776/1
type: Range
persistent identity: range2418776
display id: range2418776
version: 1
direction: direction/forward
top level: BBa_K1486025
start: 1
end: 188
orientation: inline

BBa_I11013
type: ComponentDefinition
persistent identity: BBa I11013
display id: BBa_I11013
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Modification of biobricks part BBa_B0015
creator: bu
created: 2004-09-02T11:00:00Z
modified: 2015-08-31T04:07:30Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2_
m user id: 0
owner id: 102
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: Modified BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_I11013_sequence/1

Modified BBa_B0015
type: SequenceAnnotation
persistent identity: annotation1064099
display id: annotation1064099
version: 1
top level: BBa_I11013
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_I11013/annotation1064099/range1064099/1

https://synbiohub.org/public/igem/BBa_I11013/annotation1064099/range1064099/1
type: Range
persistent identity: range1064099
display id: range1064099
version: 1
direction: direction/forward
top level: BBa_I11013
start: 1
end: 129
orientation: inline

BBa_C0450
type: ComponentDefinition
persistent identity: BBa C0450
display id: BBa_C0450
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Terminator (C0440 + C0440)
creator: T. Senthil
created: 2005-10-28T11:00:00Z
modified: 2015-08-31T04:07:24Z
discontinued: false
dominant: false
group u list: _44_
m user id: 0
owner id: 439
owning group id: 44
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Two terminators in Bgl operon that is antiterminated by bglG
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000141
role: partType/Terminator
component: BBa_C0440
sequence annotation: BBa_C0440
sequence: https://synbiohub.org/public/igem/BBa_C0450_sequence/1

BBa_C0440
type: Component
persistent identity: component1744577
display id: component1744577
version: 1
top level: BBa_C0450
access: public
definition: https://synbiohub.org/public/igem/BBa_C0440/1

BBa_C0440
type: Component
persistent identity: component1744572
display id: component1744572
version: 1
top level: BBa_C0450
access: public
definition: https://synbiohub.org/public/igem/BBa_C0440/1

BBa_C0440
type: SequenceAnnotation
persistent identity: annotation1744577
display id: annotation1744577
version: 1
top level: BBa_C0450
location: https://synbiohub.org/public/igem/BBa_C0450/annotation1744577/range1744577/1
component: BBa_C0440

https://synbiohub.org/public/igem/BBa_C0450/annotation1744577/range1744577/1
type: Range
persistent identity: range1744577
display id: range1744577
version: 1
direction: direction/forward
top level: BBa_C0450
start: 133
end: 256
orientation: inline

BBa_C0440
type: SequenceAnnotation
persistent identity: annotation1744572
display id: annotation1744572
version: 1
top level: BBa_C0450
location: https://synbiohub.org/public/igem/BBa_C0450/annotation1744572/range1744572/1
component: BBa_C0440

https://synbiohub.org/public/igem/BBa_C0450/annotation1744572/range1744572/1
type: Range
persistent identity: range1744572
display id: range1744572
version: 1
direction: direction/forward
top level: BBa_C0450
start: 1
end: 124
orientation: inline

BBa_K1031916
type: ComponentDefinition
persistent identity: BBa K1031916
display id: BBa_K1031916
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: J23114-XylS-Terminator
creator: He Shuaixin
created: 2013-09-08T11:00:00Z
modified: 2015-05-08T01:08:48Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1338_
m user id: 0
owner id: 15843
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: 114-XylS-TT
mutable notes: molecular cloning
mutable provenance: synthesis PSEUDOMONAS
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: B0015
sequence annotation: XylS
sequence annotation: J23114
sequence: https://synbiohub.org/public/igem/BBa_K1031916_sequence/1

B0015
type: SequenceAnnotation
persistent identity: annotation2360187
display id: annotation2360187
version: 1
top level: BBa_K1031916
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1031916/annotation2360187/range2360187/1

https://synbiohub.org/public/igem/BBa_K1031916/annotation2360187/range2360187/1
type: Range
persistent identity: range2360187
display id: range2360187
version: 1
direction: direction/forward
top level: BBa_K1031916
start: 1034
end: 1162
orientation: inline

XylS
type: SequenceAnnotation
persistent identity: annotation2360185
display id: annotation2360185
version: 1
top level: BBa_K1031916
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1031916/annotation2360185/range2360185/1

https://synbiohub.org/public/igem/BBa_K1031916/annotation2360185/range2360185/1
type: Range
persistent identity: range2360185
display id: range2360185
version: 1
direction: direction/forward
top level: BBa_K1031916
start: 62
end: 1027
orientation: inline

J23114
type: SequenceAnnotation
persistent identity: annotation2360184
display id: annotation2360184
version: 1
top level: BBa_K1031916
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1031916/annotation2360184/range2360184/1

https://synbiohub.org/public/igem/BBa_K1031916/annotation2360184/range2360184/1
type: Range
persistent identity: range2360184
display id: range2360184
version: 1
direction: direction/forward
top level: BBa_K1031916
start: 1
end: 35
orientation: inline

BBa_K1031520
type: ComponentDefinition
persistent identity: BBa K1031520
display id: BBa_K1031520
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Pg-B0031-sfGFP-Terminator (HpaR)
creator: He Shuaixin
created: 2013-09-08T11:00:00Z
modified: 2015-05-08T01:08:47Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1338_
m user id: 0
owner id: 15843
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Pg-sfGFP-TT (HpaR)
mutable notes: molecular cloning
mutable provenance: synthesis, lab made
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: Pg (HpaR)
sequence annotation: sfGFP
sequence annotation: B0015
sequence annotation: B0031
sequence: https://synbiohub.org/public/igem/BBa_K1031520_sequence/1

Pg (HpaR)
type: SequenceAnnotation
persistent identity: annotation2362409
display id: annotation2362409
version: 1
top level: BBa_K1031520
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1031520/annotation2362409/range2362409/1

https://synbiohub.org/public/igem/BBa_K1031520/annotation2362409/range2362409/1
type: Range
persistent identity: range2362409
display id: range2362409
version: 1
direction: direction/forward
top level: BBa_K1031520
start: 1
end: 227
orientation: inline

sfGFP
type: SequenceAnnotation
persistent identity: annotation2362411
display id: annotation2362411
version: 1
top level: BBa_K1031520
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1031520/annotation2362411/range2362411/1

https://synbiohub.org/public/igem/BBa_K1031520/annotation2362411/range2362411/1
type: Range
persistent identity: range2362411
display id: range2362411
version: 1
direction: direction/forward
top level: BBa_K1031520
start: 254
end: 976
orientation: inline

B0015
type: SequenceAnnotation
persistent identity: annotation2362412
display id: annotation2362412
version: 1
top level: BBa_K1031520
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1031520/annotation2362412/range2362412/1

https://synbiohub.org/public/igem/BBa_K1031520/annotation2362412/range2362412/1
type: Range
persistent identity: range2362412
display id: range2362412
version: 1
direction: direction/forward
top level: BBa_K1031520
start: 983
end: 1111
orientation: inline

B0031
type: SequenceAnnotation
persistent identity: annotation2362410
display id: annotation2362410
version: 1
top level: BBa_K1031520
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1031520/annotation2362410/range2362410/1

https://synbiohub.org/public/igem/BBa_K1031520/annotation2362410/range2362410/1
type: Range
persistent identity: range2362410
display id: range2362410
version: 1
direction: direction/forward
top level: BBa_K1031520
start: 234
end: 247
orientation: inline

BBa_K1031710
type: ComponentDefinition
persistent identity: BBa K1031710
display id: BBa_K1031710
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PaaX-Terminator
creator: He Shuaixin
created: 2013-09-08T11:00:00Z
modified: 2015-05-08T01:08:47Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1338_
m user id: 0
owner id: 15843
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: PaaX-TT
mutable notes: Molecular cloning
mutable provenance: PCR genome E.coli
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: BBa_B0034
sequence annotation: PaaX
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_K1031710_sequence/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2357485
display id: annotation2357485
version: 1
top level: BBa_K1031710
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1031710/annotation2357485/range2357485/1

https://synbiohub.org/public/igem/BBa_K1031710/annotation2357485/range2357485/1
type: Range
persistent identity: range2357485
display id: range2357485
version: 1
direction: direction/forward
top level: BBa_K1031710
start: 1
end: 12
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BBa_B0015
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