//rnap/prokaryote/ecoli/sigma70
type: Collection
persistent identity: rnap prokaryote ecoli sigma70
display id: rnap_prokaryote_ecoli_sigma70
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
owned by: james
owned by: myers
member: BBa_K823006
member: BBa_K1585103
member: BBa_J23114
member: Plac(rev)
member: Pnot(O44)
member: BBa_K256020
member: BBa_K136004
member: U049O11+D0
member: BBa_K180000
member: BBa_K091156
member: U097NUL+D0
member: KP3
member: OmpR
member: BBa_R2113
member: BBa_K1031743
member: BBa_K206001
member: BBa_J23108
member: BBa_K427003
member: BBa_K113009
member: BBa_K116401
member: BBa_K1886017
member: BBa_K823011
member: BBa_K112701
member: BBa_K180019
member: BBa_J23109
member: BBa_K266000
member: cII p22
member: Pnot(O12)
member: BBa_K1585102
member: LasR+PAI
member: BBa_K823005
member: NOR2
member: BBa_K136010
member: BBa_K180018
member: BBa_I739106
member: pspoIIE
member: BBa_R2201
member: U037O11+D0
member: U061O11+D0
member: BBa_I13406
member: BBa_K091101
member: BBa_K091157
member: BBa_I714924
member: Zif23
member: BBa_J23115
member: BBa_K137029
member: BBa_I756014
member: Bla
member: BBa_K112402
member: BBa_M13108
member: BBa_K292001
member: BBa_I746361
member: BBa_J64981
member: BBa_K137087
member: CreAp
member: Pmra
member: Las CIO
member: BBa_K1585110
member: BBa_I739105
member: BBa_I746363
member: BBa_K112401
member: BBa_K823004
member: BBa_K180017
member: BBa_J23107
member: BBa_K1520516
member: BBa_J64804
member: BBa_K1682004

U037O11+D0
type: ComponentDefinition
persistent identity: BBa I732446
display id: BBa_I732446
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member (U037O11+D038O22)
creator: Zhan Jian
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732446_sequence/1

luxR
type: ComponentDefinition
persistent identity: BBa R0061
display id: BBa_R0061
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter (HSL-mediated luxR repressor)
creator: Srini Devadas, David Gray, Ronny Krashinsky, Debra Lin, and Chris Zheng Liu
created: 2004-01-28T12:00:00Z
modified: 2015-05-08T01:14:15Z
discontinued: false
dominant: true
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This part involves the -10 binding site, the -35 binding site, and the twenty nucleotides between that constitute the lux box. With this part, LuxR functions as a acyl-homoserine lactone-dependent repressor. LuxR resonds to the HSL produced by LuxI, N-(3-oxohexanoyl)-HSL. The Lux box is positioned such that it partially overlaps the consensus -35 and -10 hexamers of an RNA polymerase binding site.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -35
sequence annotation: -10
sequence annotation: Lux Box
sequence: https://synbiohub.org/public/igem/BBa_R0061_sequence/1

-35
type: SequenceAnnotation
persistent identity: annotation305863
display id: annotation305863
version: 1
top level: luxR
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0061/annotation305863/range305863/1

https://synbiohub.org/public/igem/BBa_R0061/annotation305863/range305863/1
type: Range
persistent identity: range305863
display id: range305863
version: 1
direction: direction/forward
top level: luxR
start: 1
end: 6
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation305864
display id: annotation305864
version: 1
top level: luxR
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0061/annotation305864/range305864/1

https://synbiohub.org/public/igem/BBa_R0061/annotation305864/range305864/1
type: Range
persistent identity: range305864
display id: range305864
version: 1
direction: direction/forward
top level: luxR
start: 25
end: 30
orientation: inline

Lux Box
type: SequenceAnnotation
persistent identity: annotation305877
display id: annotation305877
version: 1
top level: luxR
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0061/annotation305877/range305877/1

https://synbiohub.org/public/igem/BBa_R0061/annotation305877/range305877/1
type: Range
persistent identity: range305877
display id: range305877
version: 1
direction: direction/forward
top level: luxR
start: 6
end: 25
orientation: inline

BBa_M13101
type: ComponentDefinition
persistent identity: BBa M13101
display id: BBa_M13101
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: M13K07 gene I promoter
creator: Natalie Kuldell
created: 2006-12-15T12:00:00Z
modified: 2015-05-08T01:13:55Z
discontinued: false
dominant: false
group u list: _45_
m user id: 0
owner id: 314
owning group id: 1
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This part is from the bacteriophage M13 genome, bp 3086-3132 in M13K07. It directs transcription of M13 gene VII (BBa_M13507, BBa_M13007). Its identity as a promoter was described in Gene (1980) 11:129-148 based on -10,-35 homology and fragment of genome able to bind RNAP.
mutable notes: none
mutable provenance: New England Biolabs M13K07. Sequenced in 2002 by L. Panganaban.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_M13101_sequence/1

BBa_K1585115
type: ComponentDefinition
persistent identity: BBa K1585115
display id: BBa_K1585115
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Anderson Promoter with lacI binding site
creator: Tobias Schwanemann
created: 2015-08-31T11:00:00Z
modified: 2015-09-01T02:13:08Z
discontinued: false
dominant: false
experience: experience/Fails
group u list: _2002_
m user id: 24474
owner id: 24474
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Promoters of the Anderson Promoter Collection with lacI binding site derived from [http://parts.igem.org/Part:BBa_R0010 R_0010] to make them [http://openwetware.org/wiki/IPTG IPTG] iducible.
mutable notes: We choosed lacI-binding-site of part BBa_R0010 because we assumed older parts would be more reliable. The part is shipped in backbone [http://parts.igem.org/Part:BBa_J61002 J61002] because the functionality is directly visible by adding IPTG. RFP ([http://parts.igem.org/Part:BBa_E1010 E1010]) should get expressed then.
mutable provenance: Assambly of [http://parts.igem.org/Part:BBa_J23110 J23110] and lacI-binding-site of [http://parts.igem.org/Part:BBa_R0010 R_0010] The "lacI binding site" of Part [http://parts.igem.org/Part:BBa_R0010 R_0010] differs from lacO from Part [http://parts.igem.org/Part:BBa_J33207 BBa_J33207].
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
component: BBa_K1585999
component: BBa_J23115
sequence annotation: BBa_J23115
sequence annotation: BBa_K1585999
sequence: https://synbiohub.org/public/igem/BBa_K1585115_sequence/1

BBa_K1585999
type: Component
persistent identity: component2441707
display id: component2441707
version: 1
top level: BBa_K1585115
access: public
definition: https://synbiohub.org/public/igem/BBa_K1585999/1

BBa_J23115
type: Component
persistent identity: component2441705
display id: component2441705
version: 1
top level: BBa_K1585115
access: public
definition: BBa_J23115

BBa_J23115
type: SequenceAnnotation
persistent identity: annotation2441705
display id: annotation2441705
version: 1
top level: BBa_K1585115
location: https://synbiohub.org/public/igem/BBa_K1585115/annotation2441705/range2441705/1
component: BBa_J23115

https://synbiohub.org/public/igem/BBa_K1585115/annotation2441705/range2441705/1
type: Range
persistent identity: range2441705
display id: range2441705
version: 1
direction: direction/reverse
top level: BBa_K1585115
start: 1
end: 35
orientation: inline

BBa_K1585999
type: SequenceAnnotation
persistent identity: annotation2441707
display id: annotation2441707
version: 1
top level: BBa_K1585115
location: https://synbiohub.org/public/igem/BBa_K1585115/annotation2441707/range2441707/1
component: BBa_K1585999

https://synbiohub.org/public/igem/BBa_K1585115/annotation2441707/range2441707/1
type: Range
persistent identity: range2441707
display id: range2441707
version: 1
direction: direction/reverse
top level: BBa_K1585115
start: 44
end: 78
orientation: inline

U097O11+D0
type: ComponentDefinition
persistent identity: BBa I732416
display id: BBa_I732416
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member (U097O11+D038O22)
creator: Zhan Jian
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732416_sequence/1

Pnot(O17)
type: ComponentDefinition
persistent identity: BBa I732272
display id: BBa_I732272
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member with Hybrid Operator (D001O17)
creator: Ma Rui
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:57Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732272_sequence/1

cinR
type: ComponentDefinition
persistent identity: BBa R0078
display id: BBa_R0078
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter (cinR and HSL regulated)
creator: Drew Endy
created: 2004-01-29T12:00:00Z
modified: 2015-05-08T01:14:15Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable provenance: Rhizobium leguminosarum
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: unidentified/uncharacterized CinR dependent
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_R0078_sequence/1

unidentified/uncharacterized CinR dependent
type: SequenceAnnotation
persistent identity: annotation318234
display id: annotation318234
version: 1
top level: cinR
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_R0078/annotation318234/range318234/1

https://synbiohub.org/public/igem/BBa_R0078/annotation318234/range318234/1
type: Range
persistent identity: range318234
display id: range318234
version: 1
direction: direction/forward
top level: cinR
start: 1
end: 220
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation318250
display id: annotation318250
version: 1
top level: cinR
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_R0078/annotation318250/range318250/1

https://synbiohub.org/public/igem/BBa_R0078/annotation318250/range318250/1
type: Range
persistent identity: range318250
display id: range318250
version: 1
direction: direction/forward
top level: cinR
start: 91
end: 136
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation318244
display id: annotation318244
version: 1
top level: cinR
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_R0078/annotation318244/range318244/1

https://synbiohub.org/public/igem/BBa_R0078/annotation318244/range318244/1
type: Range
persistent identity: range318244
display id: range318244
version: 1
direction: direction/forward
top level: cinR
start: 35
end: 74
orientation: inline

pspoIIE
type: ComponentDefinition
persistent identity: BBa J01005
display id: BBa_J01005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pspoIIE promoter (spo0A J01004, positive)
creator: Golden Bear
created: 2005-10-31T12:00:00Z
modified: 2015-08-31T04:08:11Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _13_
m user id: 0
owner id: 395
owning group id: 13
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: promoter from b. subtilis, activated by spo0A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J01005_sequence/1

BBa_R2114
type: ComponentDefinition
persistent identity: BBa R2114
display id: BBa_R2114
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter with operator site for C2003
creator: Reshma Shetty
created: 2006-10-22T11:00:00Z
modified: 2015-05-08T01:14:16Z
discontinued: false
dominant: false
group u list: _41_
m user id: 0
owner id: 126
owning group id: 70
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This promoter is designed to bind to C2003.
mutable notes: The placement of the operator site was designed to be near optimal for steric interference between C2003 and RNA polymerase.
mutable provenance: This part was inadvertently made while trying to make BBa_R2111. It contains a single base pair mutation in the -10 site of BBa_R2111.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -10 site
sequence annotation: transcription start site
sequence annotation: -35 site
sequence annotation: C2003 operator site
sequence: https://synbiohub.org/public/igem/BBa_R2114_sequence/1

-10 site
type: SequenceAnnotation
persistent identity: annotation1904659
display id: annotation1904659
version: 1
top level: BBa_R2114
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R2114/annotation1904659/range1904659/1

https://synbiohub.org/public/igem/BBa_R2114/annotation1904659/range1904659/1
type: Range
persistent identity: range1904659
display id: range1904659
version: 1
direction: direction/forward
top level: BBa_R2114
start: 40
end: 45
orientation: inline

transcription start site
type: SequenceAnnotation
persistent identity: annotation1904661
display id: annotation1904661
version: 1
top level: BBa_R2114
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_R2114/annotation1904661/range1904661/1

https://synbiohub.org/public/igem/BBa_R2114/annotation1904661/range1904661/1
type: Range
persistent identity: range1904661
display id: range1904661
version: 1
direction: direction/forward
top level: BBa_R2114
start: 53
end: 53
orientation: inline

-35 site
type: SequenceAnnotation
persistent identity: annotation1904660
display id: annotation1904660
version: 1
top level: BBa_R2114
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R2114/annotation1904660/range1904660/1

https://synbiohub.org/public/igem/BBa_R2114/annotation1904660/range1904660/1
type: Range
persistent identity: range1904660
display id: range1904660
version: 1
direction: direction/forward
top level: BBa_R2114
start: 17
end: 22
orientation: inline

C2003 operator site
type: SequenceAnnotation
persistent identity: annotation1904662
display id: annotation1904662
version: 1
top level: BBa_R2114
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_R2114/annotation1904662/range1904662/1

https://synbiohub.org/public/igem/BBa_R2114/annotation1904662/range1904662/1
type: Range
persistent identity: range1904662
display id: range1904662
version: 1
direction: direction/forward
top level: BBa_R2114
start: 23
end: 34
orientation: inline

BBa_K823014
type: ComponentDefinition
persistent identity: BBa K823014
display id: BBa_K823014
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Anderson promoter J23118
creator: Korinna Kraft
created: 2012-09-07T11:00:00Z
modified: 2015-07-07T11:51:43Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1081_
m user id: 4206
owner id: 12081
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Anderson promoter J23118 without RFP in backbone vector pSB1C3 to easily fuse the promoter with other reporters e.g. the lux operon.
mutable notes: no considerations
mutable provenance: Partsregistry
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -35 box
sequence annotation: -10 box
sequence annotation: J23118
sequence: https://synbiohub.org/public/igem/BBa_K823014_sequence/1

-35 box
type: SequenceAnnotation
persistent identity: annotation2182547
display id: annotation2182547
version: 1
top level: BBa_K823014
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K823014/annotation2182547/range2182547/1

https://synbiohub.org/public/igem/BBa_K823014/annotation2182547/range2182547/1
type: Range
persistent identity: range2182547
display id: range2182547
version: 1
direction: direction/forward
top level: BBa_K823014
start: 1
end: 6
orientation: inline

-10 box
type: SequenceAnnotation
persistent identity: annotation2182548
display id: annotation2182548
version: 1
top level: BBa_K823014
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K823014/annotation2182548/range2182548/1

https://synbiohub.org/public/igem/BBa_K823014/annotation2182548/range2182548/1
type: Range
persistent identity: range2182548
display id: range2182548
version: 1
direction: direction/forward
top level: BBa_K823014
start: 24
end: 29
orientation: inline

J23118
type: SequenceAnnotation
persistent identity: annotation2182546
display id: annotation2182546
version: 1
top level: BBa_K823014
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K823014/annotation2182546/range2182546/1

https://synbiohub.org/public/igem/BBa_K823014/annotation2182546/range2182546/1
type: Range
persistent identity: range2182546
display id: range2182546
version: 1
direction: direction/forward
top level: BBa_K823014
start: 1
end: 35
orientation: inline

Pnot(O61)
type: ComponentDefinition
persistent identity: BBa I732279
display id: BBa_I732279
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member with Hybrid Operator (D001O61)
creator: Ma Rui
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:57Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732279_sequence/1

BBa_K823007
type: ComponentDefinition
persistent identity: BBa K823007
display id: BBa_K823007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Anderson promoter J23103
creator: Korinna Kraft
created: 2012-09-07T11:00:00Z
modified: 2015-07-07T11:46:24Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1081_
m user id: 4206
owner id: 12081
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Anderson promoter J23103 without RFP in backbone vector pSB1C3 to easily fuse the promoter with other reporters e.g. the lux operon.
mutable notes: no considerations
mutable provenance: Partsregistry
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: J23103
sequence annotation: -35 box
sequence annotation: -10 box
sequence: https://synbiohub.org/public/igem/BBa_K823007_sequence/1

J23103
type: SequenceAnnotation
persistent identity: annotation2182515
display id: annotation2182515
version: 1
top level: BBa_K823007
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K823007/annotation2182515/range2182515/1

https://synbiohub.org/public/igem/BBa_K823007/annotation2182515/range2182515/1
type: Range
persistent identity: range2182515
display id: range2182515
version: 1
direction: direction/forward
top level: BBa_K823007
start: 1
end: 35
orientation: inline

-35 box
type: SequenceAnnotation
persistent identity: annotation2182516
display id: annotation2182516
version: 1
top level: BBa_K823007
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K823007/annotation2182516/range2182516/1

https://synbiohub.org/public/igem/BBa_K823007/annotation2182516/range2182516/1
type: Range
persistent identity: range2182516
display id: range2182516
version: 1
direction: direction/forward
top level: BBa_K823007
start: 1
end: 6
orientation: inline

-10 box
type: SequenceAnnotation
persistent identity: annotation2182517
display id: annotation2182517
version: 1
top level: BBa_K823007
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K823007/annotation2182517/range2182517/1

https://synbiohub.org/public/igem/BBa_K823007/annotation2182517/range2182517/1
type: Range
persistent identity: range2182517
display id: range2182517
version: 1
direction: direction/forward
top level: BBa_K823007
start: 24
end: 29
orientation: inline

BBa_K137089
type: ComponentDefinition
persistent identity: BBa K137089
display id: BBa_K137089
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: optimized (TA) repeat constitutive promoter with 21 bp between -10 and -35 elements
creator: Allen Lin
created: 2008-08-20T11:00:00Z
modified: 2015-05-08T01:10:10Z
discontinued: false
dominant: false
experience: experience/None
group u list: _187_
m user id: 0
owner id: 3112
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: optimized (TA) repeat constitutive promoter with 21 bp between -10 and -35 elements
mutable notes: None
mutable provenance: PCR
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K137089_sequence/1

BBa_M13105
type: ComponentDefinition
persistent identity: BBa M13105
display id: BBa_M13105
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: M13K07 gene V promoter
creator: Natalie Kuldell
created: 2006-12-15T12:00:00Z
modified: 2015-05-08T01:13:55Z
discontinued: false
dominant: false
group u list: _45_
m user id: 0
owner id: 314
owning group id: 1
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This part is from the bacteriophage M13 genome, bp 786-835 in M13K07. It directs transcription of M13 gene V (BBa_M13505, BBa_M13005). Its identity as a promoter was described in Gene (1980) 11:129-148 based on -10,-35 homology and fragment of genome able to bind RNAP.
mutable notes: none
mutable provenance: New England Biolabs M13K07. Sequenced in 2002 by L. Panganaban.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_M13105_sequence/1

BBa_K256028
type: ComponentDefinition
persistent identity: BBa K256028
display id: BBa_K256028
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: placI:CHE
creator: iGEM09_NTU-Singapore
created: 2009-10-18T11:00:00Z
modified: 2015-05-08T01:11:41Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _359_
m user id: 0
owner id: 4864
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: Generator of (CHE)Cholesterol esterase. Attached to placI, the part is inducible by IPTG and Lactose. It works better in Lactose.
mutable notes: --
mutable provenance: --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K256023
component: BBa_B0012
component: BBa_R0010
component: BBa_B0034
component: BBa_B0010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_B0034
sequence annotation: BBa_K256023
sequence annotation: BBa_R0010
sequence: https://synbiohub.org/public/igem/BBa_K256028_sequence/1

BBa_K256023
type: Component
persistent identity: component2052738
display id: component2052738
version: 1
top level: BBa_K256028
access: public
definition: https://synbiohub.org/public/igem/BBa_K256023/1

BBa_B0012
type: Component
persistent identity: component2052741
display id: component2052741
version: 1
top level: BBa_K256028
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_R0010
type: Component
persistent identity: component2052729
display id: component2052729
version: 1
top level: BBa_K256028
access: public
definition: LacI

BBa_B0034
type: Component
persistent identity: component2052737
display id: component2052737
version: 1
top level: BBa_K256028
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0010
type: Component
persistent identity: component2052739
display id: component2052739
version: 1
top level: BBa_K256028
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2052739
display id: annotation2052739
version: 1
top level: BBa_K256028
location: https://synbiohub.org/public/igem/BBa_K256028/annotation2052739/range2052739/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K256028/annotation2052739/range2052739/1
type: Range
persistent identity: range2052739
display id: range2052739
version: 1
direction: direction/forward
top level: BBa_K256028
start: 1177
end: 1256
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2052741
display id: annotation2052741
version: 1
top level: BBa_K256028
location: https://synbiohub.org/public/igem/BBa_K256028/annotation2052741/range2052741/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K256028/annotation2052741/range2052741/1
type: Range
persistent identity: range2052741
display id: range2052741
version: 1
direction: direction/forward
top level: BBa_K256028
start: 1265
end: 1305
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2052737
display id: annotation2052737
version: 1
top level: BBa_K256028
location: https://synbiohub.org/public/igem/BBa_K256028/annotation2052737/range2052737/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K256028/annotation2052737/range2052737/1
type: Range
persistent identity: range2052737
display id: range2052737
version: 1
direction: direction/reverse
top level: BBa_K256028
start: 209
end: 220
orientation: inline

BBa_K256023
type: SequenceAnnotation
persistent identity: annotation2052738
display id: annotation2052738
version: 1
top level: BBa_K256028
location: https://synbiohub.org/public/igem/BBa_K256028/annotation2052738/range2052738/1
component: BBa_K256023

https://synbiohub.org/public/igem/BBa_K256028/annotation2052738/range2052738/1
type: Range
persistent identity: range2052738
display id: range2052738
version: 1
direction: direction/reverse
top level: BBa_K256028
start: 227
end: 1168
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation2052729
display id: annotation2052729
version: 1
top level: BBa_K256028
location: https://synbiohub.org/public/igem/BBa_K256028/annotation2052729/range2052729/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_K256028/annotation2052729/range2052729/1
type: Range
persistent identity: range2052729
display id: range2052729
version: 1
direction: direction/forward
top level: BBa_K256028
start: 1
end: 200
orientation: inline

BBa_K823013
type: ComponentDefinition
persistent identity: BBa K823013
display id: BBa_K823013
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Anderson promoter J23117
creator: Korinna Kraft
created: 2012-09-07T11:00:00Z
modified: 2015-07-07T11:51:22Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1081_
m user id: 4206
owner id: 12081
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Anderson promoter J23117 without RFP in backbone vector pSB1C3 to easily fuse the promoter with other reporters e.g. the lux operon.
mutable notes: no considerations
mutable provenance: Partsregistry
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -10 box
sequence annotation: -35 box
sequence annotation: J23117
sequence: https://synbiohub.org/public/igem/BBa_K823013_sequence/1

-10 box
type: SequenceAnnotation
persistent identity: annotation2182545
display id: annotation2182545
version: 1
top level: BBa_K823013
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K823013/annotation2182545/range2182545/1

https://synbiohub.org/public/igem/BBa_K823013/annotation2182545/range2182545/1
type: Range
persistent identity: range2182545
display id: range2182545
version: 1
direction: direction/forward
top level: BBa_K823013
start: 24
end: 29
orientation: inline

-35 box
type: SequenceAnnotation
persistent identity: annotation2182544
display id: annotation2182544
version: 1
top level: BBa_K823013
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K823013/annotation2182544/range2182544/1

https://synbiohub.org/public/igem/BBa_K823013/annotation2182544/range2182544/1
type: Range
persistent identity: range2182544
display id: range2182544
version: 1
direction: direction/forward
top level: BBa_K823013
start: 1
end: 6
orientation: inline

J23117
type: SequenceAnnotation
persistent identity: annotation2182543
display id: annotation2182543
version: 1
top level: BBa_K823013
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K823013/annotation2182543/range2182543/1

https://synbiohub.org/public/igem/BBa_K823013/annotation2182543/range2182543/1
type: Range
persistent identity: range2182543
display id: range2182543
version: 1
direction: direction/forward
top level: BBa_K823013
start: 1
end: 35
orientation: inline

BBa_J15502
type: ComponentDefinition
persistent identity: BBa J15502
display id: BBa_J15502
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: copA promoter
creator: Chris French
created: 2008-05-15T11:00:00Z
modified: 2015-08-31T04:08:32Z
discontinued: false
dominant: false
group u list: _163_
m user id: 0
owner id: 837
owning group id: 163
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Promoter of the copA copper efflux gene from Escherichia coli K12. This promoter is induced in the presence of copper ions, regulated by the activator protein CueR (CopR).
mutable notes: No special considerations
mutable provenance: Escherichia coli K12
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: CpxR
sequence annotation: -35
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_J15502_sequence/1

CpxR
type: SequenceAnnotation
persistent identity: annotation1963241
display id: annotation1963241
version: 1
top level: BBa_J15502
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_J15502/annotation1963241/range1963241/1

https://synbiohub.org/public/igem/BBa_J15502/annotation1963241/range1963241/1
type: Range
persistent identity: range1963241
display id: range1963241
version: 1
direction: direction/forward
top level: BBa_J15502
start: 214
end: 227
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1963239
display id: annotation1963239
version: 1
top level: BBa_J15502
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J15502/annotation1963239/range1963239/1

https://synbiohub.org/public/igem/BBa_J15502/annotation1963239/range1963239/1
type: Range
persistent identity: range1963239
display id: range1963239
version: 1
direction: direction/forward
top level: BBa_J15502
start: 248
end: 253
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1963240
display id: annotation1963240
version: 1
top level: BBa_J15502
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J15502/annotation1963240/range1963240/1

https://synbiohub.org/public/igem/BBa_J15502/annotation1963240/range1963240/1
type: Range
persistent identity: range1963240
display id: range1963240
version: 1
direction: direction/forward
top level: BBa_J15502
start: 273
end: 278
orientation: inline

BBa_K137086
type: ComponentDefinition
persistent identity: BBa K137086
display id: BBa_K137086
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: optimized (TA) repeat constitutive promoter with 15 bp between -10 and -35 elements
creator: Allen Lin
created: 2008-08-20T11:00:00Z
modified: 2015-05-08T01:10:10Z
discontinued: false
dominant: false
experience: experience/None
group u list: _187_
m user id: 0
owner id: 3112
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: optimized (TA) repeat constitutive promoter with 15 bp between -10 and -35 elements
mutable notes: None
mutable provenance: PCR
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K137086_sequence/1

hybB
type: ComponentDefinition
persistent identity: BBa J45503
display id: BBa_J45503
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: hybB Cold Shock Promoter
creator: Stephen Payne
created: 2006-06-20T11:00:00Z
modified: 2015-08-31T04:08:49Z
discontinued: false
dominant: false
experience: experience/None
group u list: _84_
m user id: 0
owner id: 642
owning group id: 84
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This cold shock promoter is only active at temperatures lower than 30 degree Celsius.
mutable notes: None.
mutable provenance: Chris Voigt Lab at UCSF
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J45503_sequence/1

U073O11+D0
type: ComponentDefinition
persistent identity: BBa I732430
display id: BBa_I732430
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member (U073O11+D062O22)
creator: Zhan Jian
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732430_sequence/1

BBa_K180003
type: ComponentDefinition
persistent identity: BBa K180003
display id: BBa_K180003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PTAC testing plasmid (GFP) - basic
creator: Richard Wilson, Stephanie Chambers
created: 2009-10-15T11:00:00Z
modified: 2015-05-08T01:11:06Z
discontinued: false
dominant: false
experience: experience/None
group u list: _278_
m user id: 0
owner id: 4082
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: As per BBa_K180002, omitting the RBS and STOP biobrick parts (B0034 and B0015) for speed and ease of construction.
mutable notes: Assess if the RBS (B0034) sequence is needed prior to GFP (E0040) whilst still able to test function of part K180000.
mutable provenance: See parts K180000, E0040
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K180000
component: BBa_E0040
sequence annotation: BBa_K180000
sequence annotation: BBa_E0040
sequence: https://synbiohub.org/public/igem/BBa_K180003_sequence/1

BBa_K180000
type: Component
persistent identity: component2044072
display id: component2044072
version: 1
top level: BBa_K180003
access: public
definition: BBa_K180000

BBa_E0040
type: Component
persistent identity: component2044074
display id: component2044074
version: 1
top level: BBa_K180003
access: public
definition: https://synbiohub.org/public/igem/BBa_E0040/1

BBa_K180000
type: SequenceAnnotation
persistent identity: annotation2044072
display id: annotation2044072
version: 1
top level: BBa_K180003
location: https://synbiohub.org/public/igem/BBa_K180003/annotation2044072/range2044072/1
component: BBa_K180000

https://synbiohub.org/public/igem/BBa_K180003/annotation2044072/range2044072/1
type: Range
persistent identity: range2044072
display id: range2044072
version: 1
direction: direction/reverse
top level: BBa_K180003
start: 1
end: 1447
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation2044074
display id: annotation2044074
version: 1
top level: BBa_K180003
location: https://synbiohub.org/public/igem/BBa_K180003/annotation2044074/range2044074/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_K180003/annotation2044074/range2044074/1
type: Range
persistent identity: range2044074
display id: range2044074
version: 1
direction: direction/reverse
top level: BBa_K180003
start: 1454
end: 2173
orientation: inline

U049O11+D0
type: ComponentDefinition
persistent identity: BBa I732437
display id: BBa_I732437
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member (U049O11+D002O22)
creator: Zhan Jian
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732437_sequence/1

BBa_K112900
type: ComponentDefinition
persistent identity: BBa K112900
display id: BBa_K112900
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Pbad
creator: Bing Xia
created: 2008-10-20T11:00:00Z
modified: 2015-05-08T01:09:19Z
discontinued: false
dominant: false
group u list: _224_
m user id: 0
owner id: 2998
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Arabinose induced promoter. ---- This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at: [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K112900_sequence/1

BBa_I732021
type: ComponentDefinition
persistent identity: BBa I732021
display id: BBa_I732021
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Template for Building Primer Family Member
creator: Zhan Jian
created: 2007-07-26T11:00:00Z
modified: 2015-08-31T04:07:56Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1572
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -35
sequence annotation: +1
sequence annotation: consensus elements
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_I732021_sequence/1

-35
type: SequenceAnnotation
persistent identity: annotation1939927
display id: annotation1939927
version: 1
top level: BBa_I732021
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I732021/annotation1939927/range1939927/1

https://synbiohub.org/public/igem/BBa_I732021/annotation1939927/range1939927/1
type: Range
persistent identity: range1939927
display id: range1939927
version: 1
direction: direction/forward
top level: BBa_I732021
start: 62
end: 67
orientation: inline

+1
type: SequenceAnnotation
persistent identity: annotation1939929
display id: annotation1939929
version: 1
top level: BBa_I732021
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I732021/annotation1939929/range1939929/1

https://synbiohub.org/public/igem/BBa_I732021/annotation1939929/range1939929/1
type: Range
persistent identity: range1939929
display id: range1939929
version: 1
direction: direction/forward
top level: BBa_I732021
start: 98
end: 98
orientation: inline

consensus elements
type: SequenceAnnotation
persistent identity: annotation1939930
display id: annotation1939930
version: 1
top level: BBa_I732021
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_I732021/annotation1939930/range1939930/1

https://synbiohub.org/public/igem/BBa_I732021/annotation1939930/range1939930/1
type: Range
persistent identity: range1939930
display id: range1939930
version: 1
direction: direction/forward
top level: BBa_I732021
start: 62
end: 98
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1939928
display id: annotation1939928
version: 1
top level: BBa_I732021
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I732021/annotation1939928/range1939928/1

https://synbiohub.org/public/igem/BBa_I732021/annotation1939928/range1939928/1
type: Range
persistent identity: range1939928
display id: range1939928
version: 1
direction: direction/forward
top level: BBa_I732021
start: 86
end: 91
orientation: inline

BBa_K091101
type: ComponentDefinition
persistent identity: BBa K091101
display id: BBa_K091101
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pTet_Lac hybrid promoter
creator: Erin Feeney
created: 2008-05-28T11:00:00Z
modified: 2015-05-08T01:08:37Z
discontinued: false
dominant: false
experience: experience/None
group u list: _191_
m user id: 0
owner id: 3067
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This promoter is repressed by tetR and lacI; it contains two binding sites for TetR and one LacI binding site. this part is an AND-gate since transcription occurs only in the presence of both ATC and IPTG.
mutable notes: Also designed by Pallavi Penumetcha and Robert Cool.
mutable provenance: This part consists of the R0040 tetR promoter with the addition of the R0010 lacI binding site.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -35
sequence annotation: lacI binding site
sequence annotation: -10
sequence annotation: tetR binding site 1
sequence annotation: tetR binding site 2
sequence: https://synbiohub.org/public/igem/BBa_K091101_sequence/1

-35
type: SequenceAnnotation
persistent identity: annotation1963339
display id: annotation1963339
version: 1
top level: BBa_K091101
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K091101/annotation1963339/range1963339/1

https://synbiohub.org/public/igem/BBa_K091101/annotation1963339/range1963339/1
type: Range
persistent identity: range1963339
display id: range1963339
version: 1
direction: direction/forward
top level: BBa_K091101
start: 20
end: 25
orientation: inline

lacI binding site
type: SequenceAnnotation
persistent identity: annotation1963341
display id: annotation1963341
version: 1
top level: BBa_K091101
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K091101/annotation1963341/range1963341/1

https://synbiohub.org/public/igem/BBa_K091101/annotation1963341/range1963341/1
type: Range
persistent identity: range1963341
display id: range1963341
version: 1
direction: direction/forward
top level: BBa_K091101
start: 49
end: 83
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1963340
display id: annotation1963340
version: 1
top level: BBa_K091101
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K091101/annotation1963340/range1963340/1

https://synbiohub.org/public/igem/BBa_K091101/annotation1963340/range1963340/1
type: Range
persistent identity: range1963340
display id: range1963340
version: 1
direction: direction/forward
top level: BBa_K091101
start: 43
end: 48
orientation: inline

tetR binding site 1
type: SequenceAnnotation
persistent identity: annotation1963337
display id: annotation1963337
version: 1
top level: BBa_K091101
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K091101/annotation1963337/range1963337/1

https://synbiohub.org/public/igem/BBa_K091101/annotation1963337/range1963337/1
type: Range
persistent identity: range1963337
display id: range1963337
version: 1
direction: direction/forward
top level: BBa_K091101
start: 1
end: 19
orientation: inline

tetR binding site 2
type: SequenceAnnotation
persistent identity: annotation1963338
display id: annotation1963338
version: 1
top level: BBa_K091101
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K091101/annotation1963338/range1963338/1

https://synbiohub.org/public/igem/BBa_K091101/annotation1963338/range1963338/1
type: Range
persistent identity: range1963338
display id: range1963338
version: 1
direction: direction/forward
top level: BBa_K091101
start: 26
end: 44
orientation: inline

BBa_R2201
type: ComponentDefinition
persistent identity: BBa R2201
display id: BBa_R2201
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: C2006-repressible promoter
creator: Reshma Shetty
created: 2007-08-16T11:00:00Z
modified: 2015-05-08T01:14:16Z
discontinued: false
dominant: false
group u list: _41_
m user id: 0
owner id: 126
owning group id: 162
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is a promoter designed to be repressed by BBa_C2006: a MBP.Zif268.GCN4 homodimer.
mutable notes: The operator site is positioned to increase the steric hindrance between the repressor and RNAP based on comparing the structures of the repressor bound to DNA and RNAP bound to DNA.
mutable provenance: This sequence is derived from BBa_R2000 and the protein structures of Zif268.GCN4(-2) bound to DNA and RNAP bound to DNA.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -10
sequence annotation: operator
sequence annotation: -35
sequence: https://synbiohub.org/public/igem/BBa_R2201_sequence/1

-10
type: SequenceAnnotation
persistent identity: annotation1941587
display id: annotation1941587
version: 1
top level: BBa_R2201
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R2201/annotation1941587/range1941587/1

https://synbiohub.org/public/igem/BBa_R2201/annotation1941587/range1941587/1
type: Range
persistent identity: range1941587
display id: range1941587
version: 1
direction: direction/forward
top level: BBa_R2201
start: 33
end: 38
orientation: inline

operator
type: SequenceAnnotation
persistent identity: annotation1941586
display id: annotation1941586
version: 1
top level: BBa_R2201
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_R2201/annotation1941586/range1941586/1

https://synbiohub.org/public/igem/BBa_R2201/annotation1941586/range1941586/1
type: Range
persistent identity: range1941586
display id: range1941586
version: 1
direction: direction/forward
top level: BBa_R2201
start: 14
end: 26
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1941585
display id: annotation1941585
version: 1
top level: BBa_R2201
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R2201/annotation1941585/range1941585/1

https://synbiohub.org/public/igem/BBa_R2201/annotation1941585/range1941585/1
type: Range
persistent identity: range1941585
display id: range1941585
version: 1
direction: direction/forward
top level: BBa_R2201
start: 10
end: 15
orientation: inline

BBa_J54016
type: ComponentDefinition
persistent identity: BBa J54016
display id: BBa_J54016
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: promoter_lacq
creator: Yusaku Nakashima
created: 2006-10-14T11:00:00Z
modified: 2015-08-31T03:48:11Z
discontinued: false
dominant: false
group u list: _76_
m user id: 0
owner id: 762
owning group id: 76
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: lacq promoter
mutable notes: As Promoter
mutable provenance: DNA synthesis
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: promoter_lacq
sequence annotation: BamHI
sequence: https://synbiohub.org/public/igem/BBa_J54016_sequence/1

promoter_lacq
type: SequenceAnnotation
persistent identity: annotation1903169
display id: annotation1903169
version: 1
top level: BBa_J54016
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J54016/annotation1903169/range1903169/1

https://synbiohub.org/public/igem/BBa_J54016/annotation1903169/range1903169/1
type: Range
persistent identity: range1903169
display id: range1903169
version: 1
direction: direction/forward
top level: BBa_J54016
start: 18
end: 49
orientation: inline

BamHI
type: SequenceAnnotation
persistent identity: annotation1903168
display id: annotation1903168
version: 1
top level: BBa_J54016
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_J54016/annotation1903168/range1903168/1

https://synbiohub.org/public/igem/BBa_J54016/annotation1903168/range1903168/1
type: Range
persistent identity: range1903168
display id: range1903168
version: 1
direction: direction/forward
top level: BBa_J54016
start: 12
end: 17
orientation: inline

BBa_K256020
type: ComponentDefinition
persistent identity: BBa K256020
display id: BBa_K256020
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: J23119:HO1
creator: iGEM09_NTU-Singapore
created: 2009-10-18T11:00:00Z
modified: 2015-05-08T01:11:41Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _359_
m user id: 0
owner id: 4864
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: Generator of Heme Oxygenase (HO1)
mutable notes: --
mutable provenance: --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0015
component: BBa_B0034
component: BBa_J23119
component: BBa_I15008
sequence annotation: BBa_B0015
sequence annotation: BBa_B0034
sequence annotation: BBa_J23119
sequence annotation: BBa_I15008
sequence: https://synbiohub.org/public/igem/BBa_K256020_sequence/1

BBa_B0015
type: Component
persistent identity: component2256399
display id: component2256399
version: 1
top level: BBa_K256020
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_B0034
type: Component
persistent identity: component2256390
display id: component2256390
version: 1
top level: BBa_K256020
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_J23119
type: Component
persistent identity: component2256388
display id: component2256388
version: 1
top level: BBa_K256020
access: public
definition: BBa_J23119

BBa_I15008
type: Component
persistent identity: component2256392
display id: component2256392
version: 1
top level: BBa_K256020
access: public
definition: https://synbiohub.org/public/igem/BBa_I15008/1

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2256399
display id: annotation2256399
version: 1
top level: BBa_K256020
location: https://synbiohub.org/public/igem/BBa_K256020/annotation2256399/range2256399/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K256020/annotation2256399/range2256399/1
type: Range
persistent identity: range2256399
display id: range2256399
version: 1
direction: direction/reverse
top level: BBa_K256020
start: 821
end: 949
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2256390
display id: annotation2256390
version: 1
top level: BBa_K256020
location: https://synbiohub.org/public/igem/BBa_K256020/annotation2256390/range2256390/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K256020/annotation2256390/range2256390/1
type: Range
persistent identity: range2256390
display id: range2256390
version: 1
direction: direction/reverse
top level: BBa_K256020
start: 44
end: 55
orientation: inline

BBa_J23119
type: SequenceAnnotation
persistent identity: annotation2256388
display id: annotation2256388
version: 1
top level: BBa_K256020
location: https://synbiohub.org/public/igem/BBa_K256020/annotation2256388/range2256388/1
component: BBa_J23119

https://synbiohub.org/public/igem/BBa_K256020/annotation2256388/range2256388/1
type: Range
persistent identity: range2256388
display id: range2256388
version: 1
direction: direction/reverse
top level: BBa_K256020
start: 1
end: 35
orientation: inline

BBa_I15008
type: SequenceAnnotation
persistent identity: annotation2256392
display id: annotation2256392
version: 1
top level: BBa_K256020
location: https://synbiohub.org/public/igem/BBa_K256020/annotation2256392/range2256392/1
component: BBa_I15008

https://synbiohub.org/public/igem/BBa_K256020/annotation2256392/range2256392/1
type: Range
persistent identity: range2256392
display id: range2256392
version: 1
direction: direction/reverse
top level: BBa_K256020
start: 62
end: 812
orientation: inline

NAND2616
type: ComponentDefinition
persistent identity: BBa I732301
display id: BBa_I732301
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: NAND Candidate (U073O26D001O16)
creator: Ma Rui
created: 2007-10-02T11:00:00Z
modified: 2015-08-31T04:07:57Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _156_
m user id: 0
owner id: 1592
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: U97NUL D001O11
mutable notes: N/A
mutable provenance: LacIq
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732301_sequence/1

BBa_J56015
type: ComponentDefinition
persistent identity: BBa J56015
display id: BBa_J56015
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: lacIQ - promoter sequence
creator: Matt Eames
created: 2006-10-31T12:00:00Z
modified: 2015-08-31T03:22:59Z
discontinued: false
dominant: false
group u list: _79_98_
m user id: 0
owner id: 1132
owning group id: 98
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Promoter sequence for constituitive expression
mutable notes: None
mutable provenance: Synthesized by DNA 2.0
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J56015_sequence/1

Pspac-hy
type: ComponentDefinition
persistent identity: BBa I746665
display id: BBa_I746665
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Pspac-hy promoter
creator: Yue Miao
created: 2007-10-23T11:00:00Z
modified: 2015-08-31T04:08:05Z
discontinued: false
dominant: false
experience: experience/None
group u list: _116_
m user id: 0
owner id: 2119
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: There is a G->T point mutation of pSpac at the -1 site, changing the promoter to a stronger one - hence the name pSpac-hy, hy meaning hyper. This promoter drives transcription at rates of about ten times that of the wild type (pSpac) promoter.
mutable notes: NA
mutable provenance: Comes from the pPL82 vector, which is intended as a B. subtilis integrational vector. It is placed in front of the MCS in the plasmid, as it would drive the expression of the gene cloned into the MCS both in B. subtilis and E. coli.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I746665_sequence/1

NANDbeta
type: ComponentDefinition
persistent identity: BBa I732306
display id: BBa_I732306
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: NAND Candidate (U073O11D002O22)
creator: Zhan Jian
created: 2007-10-25T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: {| border="1" | |align="center"| |align="center"| |}
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732306_sequence/1

BBa_K1660005
type: ComponentDefinition
persistent identity: BBa K1660005
display id: BBa_K1660005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RFP controlled by the PompR promoter
creator: Dai Yuanyi
created: 2015-09-07T11:00:00Z
modified: 2015-09-08T12:26:12Z
discontinued: false
dominant: false
group u list: _2078_
m user id: 26933
owner id: 26933
owning group id: 9
status: status/Planning
bookmark: false
mutable description: PompR is a OmpR-controlled promoter which can be positively regulated by phosphorylated OmpR. This promoter is taken from the upstream region of ompC. Phosphorylated OmpR binds to the three operator sites and activates transcription. We insert PompR(BBa_R0082) sequences into the upstream region of rfp gene, which together is connected to vector PSB1C3 afterwards, and therefore we are able to observe the OmpR promoter efficiency through the control of light.
mutable notes: We constructed this biobrick through pcr.
mutable provenance: This part comes from the stock.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_R0082
component: BBa_K516032
sequence annotation: BBa_R0082
sequence annotation: BBa_K516032
sequence: https://synbiohub.org/public/igem/BBa_K1660005_sequence/1

BBa_R0082
type: Component
persistent identity: component2446043
display id: component2446043
version: 1
top level: BBa_K1660005
access: public
definition: OmpR

BBa_K516032
type: Component
persistent identity: component2446057
display id: component2446057
version: 1
top level: BBa_K1660005
access: public
definition: https://synbiohub.org/public/igem/BBa_K516032/1

BBa_R0082
type: SequenceAnnotation
persistent identity: annotation2446043
display id: annotation2446043
version: 1
top level: BBa_K1660005
location: https://synbiohub.org/public/igem/BBa_K1660005/annotation2446043/range2446043/1
component: BBa_R0082

https://synbiohub.org/public/igem/BBa_K1660005/annotation2446043/range2446043/1
type: Range
persistent identity: range2446043
display id: range2446043
version: 1
direction: direction/reverse
top level: BBa_K1660005
start: 1
end: 108
orientation: inline

BBa_K516032
type: SequenceAnnotation
persistent identity: annotation2446057
display id: annotation2446057
version: 1
top level: BBa_K1660005
location: https://synbiohub.org/public/igem/BBa_K1660005/annotation2446057/range2446057/1
component: BBa_K516032

https://synbiohub.org/public/igem/BBa_K1660005/annotation2446057/range2446057/1
type: Range
persistent identity: range2446057
display id: range2446057
version: 1
direction: direction/reverse
top level: BBa_K1660005
start: 117
end: 978
orientation: inline

BBa_K823011
type: ComponentDefinition
persistent identity: BBa K823011
display id: BBa_K823011
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Anderson promoter J23114
creator: Korinna Kraft
created: 2012-09-07T11:00:00Z
modified: 2015-07-07T11:50:55Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1081_
m user id: 4206
owner id: 12081
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Anderson promoter J23114 without RFP in backbone vector pSB1C3 to easily fuse the promoter with other reporters e.g. the lux operon.
mutable notes: no considerations
mutable provenance: Partsregistry
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: J23114
sequence annotation: -10 box
sequence annotation: -35 box
sequence: https://synbiohub.org/public/igem/BBa_K823011_sequence/1

J23114
type: SequenceAnnotation
persistent identity: annotation2182537
display id: annotation2182537
version: 1
top level: BBa_K823011
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K823011/annotation2182537/range2182537/1

https://synbiohub.org/public/igem/BBa_K823011/annotation2182537/range2182537/1
type: Range
persistent identity: range2182537
display id: range2182537
version: 1
direction: direction/forward
top level: BBa_K823011
start: 1
end: 35
orientation: inline

-10 box
type: SequenceAnnotation
persistent identity: annotation2182539
display id: annotation2182539
version: 1
top level: BBa_K823011
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K823011/annotation2182539/range2182539/1

https://synbiohub.org/public/igem/BBa_K823011/annotation2182539/range2182539/1
type: Range
persistent identity: range2182539
display id: range2182539
version: 1
direction: direction/forward
top level: BBa_K823011
start: 24
end: 29
orientation: inline

-35 box
type: SequenceAnnotation
persistent identity: annotation2182538
display id: annotation2182538
version: 1
top level: BBa_K823011
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K823011/annotation2182538/range2182538/1

https://synbiohub.org/public/igem/BBa_K823011/annotation2182538/range2182538/1
type: Range
persistent identity: range2182538
display id: range2182538
version: 1
direction: direction/forward
top level: BBa_K823011
start: 1
end: 6
orientation: inline

BBa_K259005
type: ComponentDefinition
persistent identity: BBa K259005
display id: BBa_K259005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: AraC Rheostat Promoter
creator: Petros Mina
created: 2009-09-02T11:00:00Z
modified: 2015-05-08T01:11:42Z
discontinued: false
dominant: false
group u list: _357_
m user id: 0
owner id: 5318
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: The AraC/pBAD promoter can be used with prokaryotic chassis to regulate expresion of proteins. When the AraC/pBAD promoter is placed upstream of your canditate translational unit (protein to be expressed) it allows control of the expression of the said protein. This is done via controlling the concentration of arabinose sugar in the environment/solution of bacterial growth. This composite part does not have an LVA degradation tag at its end, hence can be used with all prokaryotic assemblies.
mutable notes: The part was designed and assembled from the iGEM2009 kit with standard RFC10 assembly.
mutable provenance: The AraC promoter is found naturally in E.Coli at 1.52 min/centisome in the clockwise orientation. More information can be found at [http://ecogene.org/geneinfo.php?eg_id=EG10054 EcoGene].
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_R0081
component: BBa_R0080
sequence annotation: BBa_R0080
sequence annotation: BBa_R0081
sequence: https://synbiohub.org/public/igem/BBa_K259005_sequence/1

BBa_R0081
type: Component
persistent identity: component2018287
display id: component2018287
version: 1
top level: BBa_K259005
access: public
definition: https://synbiohub.org/public/igem/BBa_R0081/1

BBa_R0080
type: Component
persistent identity: component2018294
display id: component2018294
version: 1
top level: BBa_K259005
access: public
definition: AraC

BBa_R0080
type: SequenceAnnotation
persistent identity: annotation2018294
display id: annotation2018294
version: 1
top level: BBa_K259005
location: https://synbiohub.org/public/igem/BBa_K259005/annotation2018294/range2018294/1
component: BBa_R0080

https://synbiohub.org/public/igem/BBa_K259005/annotation2018294/range2018294/1
type: Range
persistent identity: range2018294
display id: range2018294
version: 1
direction: direction/reverse
top level: BBa_K259005
start: 192
end: 340
orientation: inline

BBa_R0081
type: SequenceAnnotation
persistent identity: annotation2018287
display id: annotation2018287
version: 1
top level: BBa_K259005
location: https://synbiohub.org/public/igem/BBa_K259005/annotation2018287/range2018287/1
component: BBa_R0081

https://synbiohub.org/public/igem/BBa_K259005/annotation2018287/range2018287/1
type: Range
persistent identity: range2018287
display id: range2018287
version: 1
direction: direction/reverse
top level: BBa_K259005
start: 1
end: 183
orientation: inline

BBa_J07019
type: ComponentDefinition
persistent identity: BBa J07019
display id: BBa_J07019
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: FecA Promoter (with Fur box)
creator: jxyu
created: 2005-07-12T11:00:00Z
modified: 2015-08-31T04:08:19Z
discontinued: false
dominant: true
experience: experience/None
group u list: _21_
m user id: 0
owner id: 384
owning group id: 21
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: In native e.coli, this promoter is located upstream of FecA cds. Regulated by FecI protein, which is in turn regulated by FecA.
mutable provenance: MG1655
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: Fur Box (binds Fur)
sequence annotation: -59 to -1 region
sequence annotation: FecI binding region
sequence: https://synbiohub.org/public/igem/BBa_J07019_sequence/1

Fur Box (binds Fur)
type: SequenceAnnotation
persistent identity: annotation1579238
display id: annotation1579238
version: 1
top level: BBa_J07019
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_J07019/annotation1579238/range1579238/1

https://synbiohub.org/public/igem/BBa_J07019/annotation1579238/range1579238/1
type: Range
persistent identity: range1579238
display id: range1579238
version: 1
direction: direction/forward
top level: BBa_J07019
start: 25
end: 39
orientation: inline

-59 to -1 region
type: SequenceAnnotation
persistent identity: annotation1579189
display id: annotation1579189
version: 1
top level: BBa_J07019
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J07019/annotation1579189/range1579189/1

https://synbiohub.org/public/igem/BBa_J07019/annotation1579189/range1579189/1
type: Range
persistent identity: range1579189
display id: range1579189
version: 1
direction: direction/forward
top level: BBa_J07019
start: 1
end: 60
orientation: inline

FecI binding region
type: SequenceAnnotation
persistent identity: annotation1579239
display id: annotation1579239
version: 1
top level: BBa_J07019
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_J07019/annotation1579239/range1579239/1

https://synbiohub.org/public/igem/BBa_J07019/annotation1579239/range1579239/1
type: Range
persistent identity: range1579239
display id: range1579239
version: 1
direction: direction/forward
top level: BBa_J07019
start: 70
end: 76
orientation: inline

BBa_I760005
type: ComponentDefinition
persistent identity: BBa I760005
display id: BBa_I760005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Cu-sensitive promoter
creator: Gennady Zakharov
created: 2007-08-19T11:00:00Z
modified: 2015-08-31T04:08:08Z
discontinued: false
dominant: false
experience: experience/None
group u list: _148_
m user id: 0
owner id: 2080
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Promoter from the copper-sensetive CusR/CusS two component signal system. (E.Coli)
mutable notes: Sequence from the NCBI database, with added BioBricks prefix and suffix.
mutable provenance: http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I760005_sequence/1

BBa_K112320
type: ComponentDefinition
persistent identity: BBa K112320
display id: BBa_K112320
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: {< ftsAZ promoter >} in BBb format
creator: Aron Lau
created: 2008-10-21T11:00:00Z
modified: 2015-05-08T01:09:16Z
discontinued: false
dominant: false
group u list: _224_
m user id: 0
owner id: 2999
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: ftsAZ promoter is a promoter with levels of expression are inversely proportional to the growth rate. This part is actually in BBb format. ---- This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at: [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
mutable notes: PCR al0021/al0022 on MG1655 (804bp, EcoRI/BamHI/DpnI) Sub into pBca1256 (EcoRI/BamHI, 2472+910, L) Product is pBca1256-K112320 ----------------------------------------------- al0021 Forward Biobricking of ftsAZ promoter CCATAGAATTCatgAGATCTaacgacagtgttggtgagcg al0022 Reverse Biobricking of ftsAZ promoter CGttaGGATCCgtaccaatctccagtcctactaccag
mutable provenance: MG1655
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K112320_sequence/1

BBa_K136001
type: ComponentDefinition
persistent identity: BBa K136001
display id: BBa_K136001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter of flgA gene from E. coli flagella
creator: Cyprien Maisonnier
created: 2008-08-11T11:00:00Z
modified: 2015-05-08T01:10:02Z
discontinued: true
dominant: false
experience: experience/None
group u list: _211_
m user id: 0
owner id: 3012
owning group id: 9
sample status: Discontinued
status: status/Deleted
bookmark: false
mutable description: ''fliA'' is a class 2 gene that is part of the construction of the flagella ( ''Ordering Genes in a Flagella Pathway by Analysis of Expression Kinetics from Living Bacteria'' - S. Kalir, J. McClure, K. Pabbaraju, C. Southward, M. Ronen, S. Leibler, M. G. Surette, U. Alon ). Its promoter is consitutively repressed. It is activated by FlhD4C2 and maybe by FliA with different strength ( ''Using a Quantitative Blueprint to Reprogram the Dynamics of the Flagella Gene Network'' - Shiraz Kalir and Uri Alon ). '''Work in progress :''' Deeper quantitative characterization
mutable notes: The primers used to amplify this sequence are : *Forward : GTTTCTTCGAATTCGCGGCCGCTTCTAGAGAGCATATCTCCTCCGCAGGTATCAAAAT *Reverse : GTTTCTTCCTGCAGCGGCCGCTACTAGTAACAGTATCGCGATGATCGCCACGCTACGT
mutable provenance: This part comes from the genome of ''E.coli'' K12 strain MG1655.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: FlhDC binding site 1
sequence annotation: FlhDC binding site 2
sequence annotation: Initiation start for transcription
sequence: https://synbiohub.org/public/igem/BBa_K136001_sequence/1

FlhDC binding site 1
type: SequenceAnnotation
persistent identity: annotation1971568
display id: annotation1971568
version: 1
top level: BBa_K136001
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K136001/annotation1971568/range1971568/1

https://synbiohub.org/public/igem/BBa_K136001/annotation1971568/range1971568/1
type: Range
persistent identity: range1971568
display id: range1971568
version: 1
direction: direction/forward
top level: BBa_K136001
start: 63
end: 78
orientation: inline

FlhDC binding site 2
type: SequenceAnnotation
persistent identity: annotation1971569
display id: annotation1971569
version: 1
top level: BBa_K136001
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K136001/annotation1971569/range1971569/1

https://synbiohub.org/public/igem/BBa_K136001/annotation1971569/range1971569/1
type: Range
persistent identity: range1971569
display id: range1971569
version: 1
direction: direction/forward
top level: BBa_K136001
start: 69
end: 104
orientation: inline

Initiation start for transcription
type: SequenceAnnotation
persistent identity: annotation1971570
display id: annotation1971570
version: 1
top level: BBa_K136001
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K136001/annotation1971570/range1971570/1

https://synbiohub.org/public/igem/BBa_K136001/annotation1971570/range1971570/1
type: Range
persistent identity: range1971570
display id: range1971570
version: 1
direction: direction/forward
top level: BBa_K136001
start: 138
end: 138
orientation: inline

BBa_K1031743
type: ComponentDefinition
persistent identity: BBa K1031743
display id: BBa_K1031743
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: J23113-PaaX
creator: He Shuaixin
created: 2013-09-08T11:00:00Z
modified: 2015-05-08T01:08:47Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1338_
m user id: 0
owner id: 15843
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: 113-PaaX-TT
mutable notes: molecular cloning
mutable provenance: PCR genome E.coli
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: BBa_B0034
sequence annotation: BBa_J23113
sequence annotation: PaaX
sequence: https://synbiohub.org/public/igem/BBa_K1031743_sequence/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2357492
display id: annotation2357492
version: 1
top level: BBa_K1031743
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1031743/annotation2357492/range2357492/1

https://synbiohub.org/public/igem/BBa_K1031743/annotation2357492/range2357492/1
type: Range
persistent identity: range2357492
display id: range2357492
version: 1
direction: direction/forward
top level: BBa_K1031743
start: 44
end: 55
orientation: inline

BBa_J23113
type: SequenceAnnotation
persistent identity: annotation2357491
display id: annotation2357491
version: 1
top level: BBa_K1031743
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1031743/annotation2357491/range2357491/1

https://synbiohub.org/public/igem/BBa_K1031743/annotation2357491/range2357491/1
type: Range
persistent identity: range2357491
display id: range2357491
version: 1
direction: direction/forward
top level: BBa_K1031743
start: 1
end: 35
orientation: inline

PaaX
type: SequenceAnnotation
persistent identity: annotation2357493
display id: annotation2357493
version: 1
top level: BBa_K1031743
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1031743/annotation2357493/range2357493/1

https://synbiohub.org/public/igem/BBa_K1031743/annotation2357493/range2357493/1
type: Range
persistent identity: range2357493
display id: range2357493
version: 1
direction: direction/forward
top level: BBa_K1031743
start: 62
end: 1012
orientation: inline

BBa_J23116
type: ComponentDefinition
persistent identity: BBa J23116
display id: BBa_J23116
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: constitutive promoter family member
creator: John Anderson
created: 2006-08-16T11:00:00Z
modified: 2015-08-31T04:08:40Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: true
mutable description: Later
mutable notes: N/A
mutable provenance: Later
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J23116_sequence/1

BBa_I746360
type: ComponentDefinition
persistent identity: BBa I746360
display id: BBa_I746360
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PF promoter from P2 phage
creator: Stefan Milde
created: 2007-09-10T11:00:00Z
modified: 2015-08-31T04:08:04Z
discontinued: false
dominant: false
experience: experience/None
group u list: _116_
m user id: 0
owner id: 2122
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This is the PF promoter taken from the P2 phage genome. It is an inducible promoter that is activated by a class of activators, including P2 ogr (I746350), PSP3 pag (I746351) and phiR73 delta (I746352). These different activators should cause different levels of activity of the PF promoter.
mutable notes: no special considerations seemed necessary
mutable provenance: The original source is the P2 phage genome. For our work the DNA came from plasmids, kindly supplied to us by Prof. Calendar, University of California (Berkeley): Bacteriophage PSP3 and fR73 Activator Proteins: Analysis of Promoter Specificities; Julien and Calendar, 1996; JOURNAL OF BACTERIOLOGY, Oct. 1996, p. 5668???5675
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: PF
sequence: https://synbiohub.org/public/igem/BBa_I746360_sequence/1

PF
type: SequenceAnnotation
persistent identity: annotation1943783
display id: annotation1943783
version: 1
top level: BBa_I746360
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I746360/annotation1943783/range1943783/1

https://synbiohub.org/public/igem/BBa_I746360/annotation1943783/range1943783/1
type: Range
persistent identity: range1943783
display id: range1943783
version: 1
direction: direction/forward
top level: BBa_I746360
start: 1
end: 91
orientation: inline

BBa_J56012
type: ComponentDefinition
persistent identity: BBa J56012
display id: BBa_J56012
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Invertible sequence of dna includes Ptrc promoter
creator: Matt Eames
created: 2006-10-31T12:00:00Z
modified: 2015-08-31T03:22:59Z
discontinued: false
dominant: false
group u list: _79_98_
m user id: 0
owner id: 1132
owning group id: 98
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Seuqence is inverted by the FimE protein. Inside recognition sequence is a Ptrc promoter sequence. Switch is irreversible.
mutable notes: None
mutable provenance: Synthesized by DNA 2.0
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J56012_sequence/1

BBa_K266011
type: ComponentDefinition
persistent identity: BBa K266011
display id: BBa_K266011
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Lac Inverter and strong RBS
creator: UNAM FCIENCIAS-CINVESTAV IRA
created: 2009-10-13T11:00:00Z
modified: 2015-05-08T01:11:43Z
discontinued: false
dominant: false
experience: experience/None
group u list: _363_
m user id: 0
owner id: 5491
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: POPS regulated and Lac inverter system with strong RBS
mutable notes: None
mutable provenance: The biobricks BBa_Q04121 and BBa_B0034 where used to build this biobrick
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Inverter
component: BBa_Q04121
component: BBa_B0034
sequence annotation: BBa_Q04121
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K266011_sequence/1

BBa_Q04121
type: Component
persistent identity: component2262983
display id: component2262983
version: 1
top level: BBa_K266011
access: public
definition: https://synbiohub.org/public/igem/BBa_Q04121/1

BBa_B0034
type: Component
persistent identity: component2262985
display id: component2262985
version: 1
top level: BBa_K266011
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_Q04121
type: SequenceAnnotation
persistent identity: annotation2262983
display id: annotation2262983
version: 1
top level: BBa_K266011
location: https://synbiohub.org/public/igem/BBa_K266011/annotation2262983/range2262983/1
component: BBa_Q04121

https://synbiohub.org/public/igem/BBa_K266011/annotation2262983/range2262983/1
type: Range
persistent identity: range2262983
display id: range2262983
version: 1
direction: direction/reverse
top level: BBa_K266011
start: 1
end: 1371
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2262985
display id: annotation2262985
version: 1
top level: BBa_K266011
location: https://synbiohub.org/public/igem/BBa_K266011/annotation2262985/range2262985/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K266011/annotation2262985/range2262985/1
type: Range
persistent identity: range2262985
display id: range2262985
version: 1
direction: direction/reverse
top level: BBa_K266011
start: 1380
end: 1391
orientation: inline

BBa_I756014
type: ComponentDefinition
persistent identity: BBa I756014
display id: BBa_I756014
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LexAoperator-MajorLatePromoter
creator: Johannes Hugo Decker
created: 2007-08-15T11:00:00Z
modified: 2015-08-31T04:08:06Z
discontinued: false
dominant: false
group u list: _108_
m user id: 0
owner id: 2071
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This part contains the LexAoperator and MajorLatePromoter genes with restriction site KdeI at the 5 prime end and AscI at the 3' end. Will use it as both a regulatory part for certain signals as well as a promoter.
mutable notes: Had to make sure the restriction sites used were not in the sequence or in the sequence of any of the genes downstream of the promoter.
mutable provenance: genbank
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I756014_sequence/1

BBa_K131001
type: ComponentDefinition
persistent identity: BBa K131001
display id: BBa_K131001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: ColE2 SOS Promoter
creator: Kevin McLeod
created: 2008-07-21T11:00:00Z
modified: 2015-05-08T01:09:47Z
discontinued: true
dominant: false
experience: experience/None
group u list: _259_
m user id: 0
owner id: 1917
owning group id: 9
sample status: Discontinued
status: status/Deleted
bookmark: false
mutable description: This promoter normally represses the ColE2 activity gene, ceaB, in the ColE2 operon. To activate this promoter would require a RecA positive strain, as the protein LexA represses the promoter. To induce this promoter, an intercalating agent, mitomycin C, was utilized to induce the SOS response, and thus the expression of the genes under control of this promoter.
mutable notes: Lab strains of E. coli, such as Top10 from Invitrogen, are deficient in RecA so we required a RecA positive strain to induce this promoter. We used BL21 from Novagen.
mutable provenance: This part comes from the ColE2 operon, which came from a plasmid.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -15 Signal
sequence annotation: Region of Dyad Symmetry
sequence annotation: SOS Box
sequence annotation: -35 Signal
sequence: https://synbiohub.org/public/igem/BBa_K131001_sequence/1

-15 Signal
type: SequenceAnnotation
persistent identity: annotation1968165
display id: annotation1968165
version: 1
top level: BBa_K131001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K131001/annotation1968165/range1968165/1

https://synbiohub.org/public/igem/BBa_K131001/annotation1968165/range1968165/1
type: Range
persistent identity: range1968165
display id: range1968165
version: 1
direction: direction/forward
top level: BBa_K131001
start: 241
end: 246
orientation: inline

Region of Dyad Symmetry
type: SequenceAnnotation
persistent identity: annotation1968166
display id: annotation1968166
version: 1
top level: BBa_K131001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K131001/annotation1968166/range1968166/1

https://synbiohub.org/public/igem/BBa_K131001/annotation1968166/range1968166/1
type: Range
persistent identity: range1968166
display id: range1968166
version: 1
direction: direction/forward
top level: BBa_K131001
start: 136
end: 161
orientation: inline

SOS Box
type: SequenceAnnotation
persistent identity: annotation1968167
display id: annotation1968167
version: 1
top level: BBa_K131001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K131001/annotation1968167/range1968167/1

https://synbiohub.org/public/igem/BBa_K131001/annotation1968167/range1968167/1
type: Range
persistent identity: range1968167
display id: range1968167
version: 1
direction: direction/forward
top level: BBa_K131001
start: 252
end: 267
orientation: inline

-35 Signal
type: SequenceAnnotation
persistent identity: annotation1968164
display id: annotation1968164
version: 1
top level: BBa_K131001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K131001/annotation1968164/range1968164/1

https://synbiohub.org/public/igem/BBa_K131001/annotation1968164/range1968164/1
type: Range
persistent identity: range1968164
display id: range1968164
version: 1
direction: direction/forward
top level: BBa_K131001
start: 218
end: 224
orientation: inline

NAND2-7
type: ComponentDefinition
persistent identity: BBa I732304
display id: BBa_I732304
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: NAND Candidate (U073O22D001O47)
creator: Ma Rui
created: 2007-10-02T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1592
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: U97NUL D001O44
mutable notes: .
mutable provenance: .
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732304_sequence/1

BBa_S03385
type: ComponentDefinition
persistent identity: BBa S03385
display id: BBa_S03385
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Cold-sensing promoter (hybB)
creator: Elizabeth Clarke
created: 2005-11-03T12:00:00Z
modified: 2015-05-08T01:14:22Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _9_
m user id: 0
owner id: 473
owning group id: 9
sample status: No part sequence
status: status/Planning
bookmark: false
mutable description: This is the hybB promoter, which normally controlls hydrogenase regulation at low temperatures. It turns ON at 30oC and is active as low as 20oC (lower not tested). It is OFF at 37 and higher temperatures. In the literature, it is also active in anaerobic environments. This hasn't been tested.
mutable provenance: lizjaneclarke@gmail.com Univerity of California - San Francisco
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory

BBa_K137030
type: ComponentDefinition
persistent identity: BBa K137030
display id: BBa_K137030
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: constitutive promoter with (TA)9 between -10 and -35 elements
creator: Allen Lin
created: 2008-07-20T11:00:00Z
modified: 2015-05-08T01:10:09Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _187_
m user id: 0
owner id: 3112
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: constitutive promoter with (TA)9 between -10 and -35 elements
mutable notes: With 9 TA repeats between the -10 and -35 elements, the distance between the two elements is not optimal for the sigma factor to bind to the promoter. Thus, the promoter should be in the 'off' state.
mutable provenance: This part was PCR amplified from two synthesized primers that bound to each other.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: TA repeat
sequence annotation: -10 element
sequence: https://synbiohub.org/public/igem/BBa_K137030_sequence/1

TA repeat
type: SequenceAnnotation
persistent identity: annotation1967767
display id: annotation1967767
version: 1
top level: BBa_K137030
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K137030/annotation1967767/range1967767/1

https://synbiohub.org/public/igem/BBa_K137030/annotation1967767/range1967767/1
type: Range
persistent identity: range1967767
display id: range1967767
version: 1
direction: direction/forward
top level: BBa_K137030
start: 7
end: 20
orientation: inline

-10 element
type: SequenceAnnotation
persistent identity: annotation1967766
display id: annotation1967766
version: 1
top level: BBa_K137030
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K137030/annotation1967766/range1967766/1

https://synbiohub.org/public/igem/BBa_K137030/annotation1967766/range1967766/1
type: Range
persistent identity: range1967766
display id: range1967766
version: 1
direction: direction/forward
top level: BBa_K137030
start: 21
end: 25
orientation: inline

BBa_M31519
type: ComponentDefinition
persistent identity: BBa M31519
display id: BBa_M31519
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Modified promoter sequence of g3.
creator: Han Zhu
created: 2007-02-27T12:00:00Z
modified: 2015-05-08T01:14:00Z
discontinued: false
dominant: false
group u list: _102_
m user id: 0
owner id: 1377
owning group id: 102
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is the promoter sequence of g3 which has been separated from g8. It also includes the restriction enzyme EcoRV in front.
mutable notes: This part used in conjunction with BBa_M131520 allows g8 and promoter of g3 to be separate from each other, therefore allowing free regulation of g3 with no consequences on g8.
mutable provenance: GATATCaattcacctcgaaagcaagctgataaaccgatacaattaaaggctcct
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BmtI
sequence annotation: promoter-g3
sequence annotation: EcoRV
sequence: https://synbiohub.org/public/igem/BBa_M31519_sequence/1

BmtI
type: SequenceAnnotation
persistent identity: annotation1919691
display id: annotation1919691
version: 1
top level: BBa_M31519
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_M31519/annotation1919691/range1919691/1

https://synbiohub.org/public/igem/BBa_M31519/annotation1919691/range1919691/1
type: Range
persistent identity: range1919691
display id: range1919691
version: 1
direction: direction/forward
top level: BBa_M31519
start: 54
end: 60
orientation: inline

promoter-g3
type: SequenceAnnotation
persistent identity: annotation1915216
display id: annotation1915216
version: 1
top level: BBa_M31519
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_M31519/annotation1915216/range1915216/1

https://synbiohub.org/public/igem/BBa_M31519/annotation1915216/range1915216/1
type: Range
persistent identity: range1915216
display id: range1915216
version: 1
direction: direction/forward
top level: BBa_M31519
start: 7
end: 54
orientation: inline

EcoRV
type: SequenceAnnotation
persistent identity: annotation1915217
display id: annotation1915217
version: 1
top level: BBa_M31519
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_M31519/annotation1915217/range1915217/1

https://synbiohub.org/public/igem/BBa_M31519/annotation1915217/range1915217/1
type: Range
persistent identity: range1915217
display id: range1915217
version: 1
direction: direction/forward
top level: BBa_M31519
start: 1
end: 6
orientation: inline

cII p22
type: ComponentDefinition
persistent identity: BBa R0053
display id: BBa_R0053
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter (p22 cII regulated)
creator: Maia Mahoney
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: The p22 cII regulatory region sequence is a 97 base-pair sequence with the standard BioBrick prefix and suffix sections on its ends. p22 cII repressor protein, BBa_C0053, binds to it. This segment contains O-R1, O-R2, a fragment of O-R3, the -35 of P-RM, and P-R (-10 and -35 from Tom Knight)
mutable provenance: Bacteriophage p22.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: OR2
sequence annotation: OR3
sequence annotation: -10
sequence annotation: BBa_R0053
sequence annotation: -35
sequence annotation: OR1
sequence: https://synbiohub.org/public/igem/BBa_R0053_sequence/1

OR2
type: SequenceAnnotation
persistent identity: annotation2036
display id: annotation2036
version: 1
top level: cII p22
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0053/annotation2036/range2036/1

https://synbiohub.org/public/igem/BBa_R0053/annotation2036/range2036/1
type: Range
persistent identity: range2036
display id: range2036
version: 1
direction: direction/forward
top level: cII p22
start: 11
end: 28
orientation: inline

OR3
type: SequenceAnnotation
persistent identity: annotation2035
display id: annotation2035
version: 1
top level: cII p22
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0053/annotation2035/range2035/1

https://synbiohub.org/public/igem/BBa_R0053/annotation2035/range2035/1
type: Range
persistent identity: range2035
display id: range2035
version: 1
direction: direction/forward
top level: cII p22
start: 1
end: 3
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2042
display id: annotation2042
version: 1
top level: cII p22
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0053/annotation2042/range2042/1

https://synbiohub.org/public/igem/BBa_R0053/annotation2042/range2042/1
type: Range
persistent identity: range2042
display id: range2042
version: 1
direction: direction/forward
top level: cII p22
start: 30
end: 35
orientation: inline

BBa_R0053
type: SequenceAnnotation
persistent identity: annotation7069
display id: annotation7069
version: 1
top level: cII p22
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_R0053/annotation7069/range7069/1

https://synbiohub.org/public/igem/BBa_R0053/annotation7069/range7069/1
type: Range
persistent identity: range7069
display id: range7069
version: 1
direction: direction/forward
top level: cII p22
start: 1
end: 54
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2038
display id: annotation2038
version: 1
top level: cII p22
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0053/annotation2038/range2038/1

https://synbiohub.org/public/igem/BBa_R0053/annotation2038/range2038/1
type: Range
persistent identity: range2038
display id: range2038
version: 1
direction: direction/forward
top level: cII p22
start: 18
end: 23
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2041
display id: annotation2041
version: 1
top level: cII p22
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0053/annotation2041/range2041/1

https://synbiohub.org/public/igem/BBa_R0053/annotation2041/range2041/1
type: Range
persistent identity: range2041
display id: range2041
version: 1
direction: direction/forward
top level: cII p22
start: 8
end: 13
orientation: inline

OR1
type: SequenceAnnotation
persistent identity: annotation2037
display id: annotation2037
version: 1
top level: cII p22
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0053/annotation2037/range2037/1

https://synbiohub.org/public/igem/BBa_R0053/annotation2037/range2037/1
type: Range
persistent identity: range2037
display id: range2037
version: 1
direction: direction/forward
top level: cII p22
start: 34
end: 51
orientation: inline

BBa_K109200
type: ComponentDefinition
persistent identity: BBa K109200
display id: BBa_K109200
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: AraC and TetR promoter (hybrid)
creator: Faifan Tantakitti
created: 2008-07-27T11:00:00Z
modified: 2015-05-08T01:09:07Z
discontinued: false
dominant: false
experience: experience/None
group u list: _250_
m user id: 0
owner id: 3345
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This is lacI. trial version.
mutable notes: ......blank2....
mutable provenance: .....blank1...
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: AraC
sequence annotation: TetR
sequence annotation: -35
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_K109200_sequence/1

AraC
type: SequenceAnnotation
persistent identity: annotation1969061
display id: annotation1969061
version: 1
top level: BBa_K109200
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K109200/annotation1969061/range1969061/1

https://synbiohub.org/public/igem/BBa_K109200/annotation1969061/range1969061/1
type: Range
persistent identity: range1969061
display id: range1969061
version: 1
direction: direction/forward
top level: BBa_K109200
start: 34
end: 50
orientation: inline

TetR
type: SequenceAnnotation
persistent identity: annotation1969066
display id: annotation1969066
version: 1
top level: BBa_K109200
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K109200/annotation1969066/range1969066/1

https://synbiohub.org/public/igem/BBa_K109200/annotation1969066/range1969066/1
type: Range
persistent identity: range1969066
display id: range1969066
version: 1
direction: direction/forward
top level: BBa_K109200
start: 81
end: 99
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1980470
display id: annotation1980470
version: 1
top level: BBa_K109200
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K109200/annotation1980470/range1980470/1

https://synbiohub.org/public/igem/BBa_K109200/annotation1980470/range1980470/1
type: Range
persistent identity: range1980470
display id: range1980470
version: 1
direction: direction/forward
top level: BBa_K109200
start: 49
end: 54
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1980471
display id: annotation1980471
version: 1
top level: BBa_K109200
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K109200/annotation1980471/range1980471/1

https://synbiohub.org/public/igem/BBa_K109200/annotation1980471/range1980471/1
type: Range
persistent identity: range1980471
display id: range1980471
version: 1
direction: direction/forward
top level: BBa_K109200
start: 73
end: 78
orientation: inline

NAND59
type: ComponentDefinition
persistent identity: BBa I732305
display id: BBa_I732305
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: NAND Candidate (U073O22D059O46)
creator: Ma Rui
created: 2007-10-02T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _156_
m user id: 0
owner id: 1592
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: U97NUL D001O55
mutable notes: .
mutable provenance: .
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732305_sequence/1

BBa_K1585113
type: ComponentDefinition
persistent identity: BBa K1585113
display id: BBa_K1585113
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Anderson Promoter with lacI binding site
creator: Tobias Schwanemann
created: 2015-08-31T11:00:00Z
modified: 2015-09-01T02:09:36Z
discontinued: false
dominant: false
experience: experience/Fails
group u list: _2002_
m user id: 24474
owner id: 24474
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Promoters of the Anderson Promoter Collection with lacI binding site derived from [http://parts.igem.org/Part:BBa_R0010 R_0010] to make them [http://openwetware.org/wiki/IPTG IPTG] iducible.
mutable notes: We choosed lacI-binding-site of part BBa_R0010 because we assumed older parts would be more reliable. The part is shipped in backbone [http://parts.igem.org/Part:BBa_J61002 J61002] because the functionality is directly visible by adding IPTG. RFP ([http://parts.igem.org/Part:BBa_E1010 E1010]) should get expressed then.
mutable provenance: Assambly of [http://parts.igem.org/Part:BBa_J23110 J23110] and lacI-binding-site of [http://parts.igem.org/Part:BBa_R0010 R_0010] The "lacI binding site" of Part [http://parts.igem.org/Part:BBa_R0010 R_0010] differs from lacO from Part [http://parts.igem.org/Part:BBa_J33207 BBa_J33207].
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
component: BBa_K1585999
component: BBa_J23113
sequence annotation: BBa_K1585999
sequence annotation: BBa_J23113
sequence: https://synbiohub.org/public/igem/BBa_K1585113_sequence/1

BBa_K1585999
type: Component
persistent identity: component2441704
display id: component2441704
version: 1
top level: BBa_K1585113
access: public
definition: https://synbiohub.org/public/igem/BBa_K1585999/1

BBa_J23113
type: Component
persistent identity: component2441702
display id: component2441702
version: 1
top level: BBa_K1585113
access: public
definition: BBa_J23113

BBa_K1585999
type: SequenceAnnotation
persistent identity: annotation2441704
display id: annotation2441704
version: 1
top level: BBa_K1585113
location: https://synbiohub.org/public/igem/BBa_K1585113/annotation2441704/range2441704/1
component: BBa_K1585999

https://synbiohub.org/public/igem/BBa_K1585113/annotation2441704/range2441704/1
type: Range
persistent identity: range2441704
display id: range2441704
version: 1
direction: direction/reverse
top level: BBa_K1585113
start: 44
end: 78
orientation: inline

BBa_J23113
type: SequenceAnnotation
persistent identity: annotation2441702
display id: annotation2441702
version: 1
top level: BBa_K1585113
location: https://synbiohub.org/public/igem/BBa_K1585113/annotation2441702/range2441702/1
component: BBa_J23113

https://synbiohub.org/public/igem/BBa_K1585113/annotation2441702/range2441702/1
type: Range
persistent identity: range2441702
display id: range2441702
version: 1
direction: direction/reverse
top level: BBa_K1585113
start: 1
end: 35
orientation: inline

BBa_K093008
type: ComponentDefinition
persistent identity: BBa K093008
display id: BBa_K093008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: reverse BBa_R0011
creator: John Heil
created: 2008-10-27T12:00:00Z
modified: 2015-05-08T01:08:40Z
discontinued: false
dominant: false
experience: experience/None
group u list: _247_
m user id: 0
owner id: 3630
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: reverse compliments of BBa_R0011
mutable notes: for downstream application
mutable provenance: synthetic
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: lacO1
sequence annotation: -10
sequence annotation: -35
sequence: https://synbiohub.org/public/igem/BBa_K093008_sequence/1

lacO1
type: SequenceAnnotation
persistent identity: annotation1992542
display id: annotation1992542
version: 1
top level: BBa_K093008
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K093008/annotation1992542/range1992542/1

https://synbiohub.org/public/igem/BBa_K093008/annotation1992542/range1992542/1
type: Range
persistent identity: range1992542
display id: range1992542
version: 1
direction: direction/reverse
top level: BBa_K093008
start: 36
end: 52
orientation: inline

lacO1
type: SequenceAnnotation
persistent identity: annotation1992543
display id: annotation1992543
version: 1
top level: BBa_K093008
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K093008/annotation1992543/range1992543/1

https://synbiohub.org/public/igem/BBa_K093008/annotation1992543/range1992543/1
type: Range
persistent identity: range1992543
display id: range1992543
version: 1
direction: direction/reverse
top level: BBa_K093008
start: 15
end: 29
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1992540
display id: annotation1992540
version: 1
top level: BBa_K093008
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K093008/annotation1992540/range1992540/1

https://synbiohub.org/public/igem/BBa_K093008/annotation1992540/range1992540/1
type: Range
persistent identity: range1992540
display id: range1992540
version: 1
direction: direction/reverse
top level: BBa_K093008
start: 8
end: 14
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1992541
display id: annotation1992541
version: 1
top level: BBa_K093008
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K093008/annotation1992541/range1992541/1

https://synbiohub.org/public/igem/BBa_K093008/annotation1992541/range1992541/1
type: Range
persistent identity: range1992541
display id: range1992541
version: 1
direction: direction/reverse
top level: BBa_K093008
start: 30
end: 35
orientation: inline

pAND
type: ComponentDefinition
persistent identity: BBa J58100
display id: BBa_J58100
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: AND-type promoter synergistically activated by cI and CRP
creator: Valencia iGEM 2006
created: 2006-10-25T11:00:00Z
modified: 2015-08-31T03:14:48Z
discontinued: false
dominant: false
experience: experience/None
group u list: _83_
m user id: 0
owner id: 1100
owning group id: 83
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: none
mutable notes: none
mutable provenance: none
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: lam-cI OR2 binding site
sequence annotation: spacer from I12007 (77-82)
sequence annotation: -10
sequence annotation: spacer from I12007 (54-70)
sequence annotation: spacer from I12007 (26-32)
sequence annotation: spacer from I12007 (1-11, 59-72)
sequence annotation: CRP binding site
sequence annotation: -35
sequence: https://synbiohub.org/public/igem/BBa_J58100_sequence/1

lam-cI OR2 binding site
type: SequenceAnnotation
persistent identity: annotation1908169
display id: annotation1908169
version: 1
top level: pAND
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_J58100/annotation1908169/range1908169/1

https://synbiohub.org/public/igem/BBa_J58100/annotation1908169/range1908169/1
type: Range
persistent identity: range1908169
display id: range1908169
version: 1
direction: direction/forward
top level: pAND
start: 57
end: 74
orientation: inline

spacer from I12007 (77-82)
type: SequenceAnnotation
persistent identity: annotation1908182
display id: annotation1908182
version: 1
top level: pAND
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J58100/annotation1908182/range1908182/1

https://synbiohub.org/public/igem/BBa_J58100/annotation1908182/range1908182/1
type: Range
persistent identity: range1908182
display id: range1908182
version: 1
direction: direction/forward
top level: pAND
start: 101
end: 106
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1908179
display id: annotation1908179
version: 1
top level: pAND
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J58100/annotation1908179/range1908179/1

https://synbiohub.org/public/igem/BBa_J58100/annotation1908179/range1908179/1
type: Range
persistent identity: range1908179
display id: range1908179
version: 1
direction: direction/forward
top level: pAND
start: 95
end: 100
orientation: inline

spacer from I12007 (54-70)
type: SequenceAnnotation
persistent identity: annotation1908181
display id: annotation1908181
version: 1
top level: pAND
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J58100/annotation1908181/range1908181/1

https://synbiohub.org/public/igem/BBa_J58100/annotation1908181/range1908181/1
type: Range
persistent identity: range1908181
display id: range1908181
version: 1
direction: direction/forward
top level: pAND
start: 78
end: 94
orientation: inline

spacer from I12007 (26-32)
type: SequenceAnnotation
persistent identity: annotation1908180
display id: annotation1908180
version: 1
top level: pAND
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J58100/annotation1908180/range1908180/1

https://synbiohub.org/public/igem/BBa_J58100/annotation1908180/range1908180/1
type: Range
persistent identity: range1908180
display id: range1908180
version: 1
direction: direction/forward
top level: pAND
start: 50
end: 56
orientation: inline

spacer from I12007 (1-11, 59-72)
type: SequenceAnnotation
persistent identity: annotation1908171
display id: annotation1908171
version: 1
top level: pAND
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J58100/annotation1908171/range1908171/1

https://synbiohub.org/public/igem/BBa_J58100/annotation1908171/range1908171/1
type: Range
persistent identity: range1908171
display id: range1908171
version: 1
direction: direction/forward
top level: pAND
start: 25
end: 49
orientation: inline

CRP binding site
type: SequenceAnnotation
persistent identity: annotation1908168
display id: annotation1908168
version: 1
top level: pAND
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_J58100/annotation1908168/range1908168/1

https://synbiohub.org/public/igem/BBa_J58100/annotation1908168/range1908168/1
type: Range
persistent identity: range1908168
display id: range1908168
version: 1
direction: direction/forward
top level: pAND
start: 3
end: 24
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1908178
display id: annotation1908178
version: 1
top level: pAND
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J58100/annotation1908178/range1908178/1

https://synbiohub.org/public/igem/BBa_J58100/annotation1908178/range1908178/1
type: Range
persistent identity: range1908178
display id: range1908178
version: 1
direction: direction/forward
top level: pAND
start: 72
end: 77
orientation: inline

BBa_K819002
type: ComponentDefinition
persistent identity: BBa K819002
display id: BBa_K819002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Luminesensor repressible recA408 promoter
creator: YU Zhou
created: 2012-09-01T11:00:00Z
modified: 2015-05-08T01:13:29Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1077_
m user id: 0
owner id: 13785
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: recA promoter in 408 form.recA promoter belongs to the SOS regulon family, and promotes in E.coli transcription of recA protein, which forms filament when bound to ssDNA and trigger the self-cleavage of LexA protein.recA408 have one SOS box, which binds to the dimerized LexA protein and cause inhibition of the transcription of downstream gene.
mutable notes: The original RecA was mutated to 408 form in order to be responsive to 408 mutant form of LexA DNA binding domain.
mutable provenance: This comes later.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: RecA 408 promoter
sequence annotation: transcription start site
sequence: https://synbiohub.org/public/igem/BBa_K819002_sequence/1

RecA 408 promoter
type: SequenceAnnotation
persistent identity: annotation2181609
display id: annotation2181609
version: 1
top level: BBa_K819002
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K819002/annotation2181609/range2181609/1

https://synbiohub.org/public/igem/BBa_K819002/annotation2181609/range2181609/1
type: Range
persistent identity: range2181609
display id: range2181609
version: 1
direction: direction/forward
top level: BBa_K819002
start: 1
end: 81
orientation: inline

transcription start site
type: SequenceAnnotation
persistent identity: annotation2181610
display id: annotation2181610
version: 1
top level: BBa_K819002
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K819002/annotation2181610/range2181610/1

https://synbiohub.org/public/igem/BBa_K819002/annotation2181610/range2181610/1
type: Range
persistent identity: range2181610
display id: range2181610
version: 1
direction: direction/forward
top level: BBa_K819002
start: 61
end: 61
orientation: inline

BBa_K137124
type: ComponentDefinition
persistent identity: BBa K137124
display id: BBa_K137124
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LacI-repressed promoter A81
creator: Josh Michener
created: 2008-10-07T11:00:00Z
modified: 2015-05-08T01:10:11Z
discontinued: true
dominant: false
experience: experience/None
group u list: _187_
m user id: 0
owner id: 253
owning group id: 6
sample status: Discontinued
status: status/Deleted
bookmark: false
mutable description: LacI-repressible promoter A81 developed by Sidney Cox (http://www.nature.com/msb/journal/v3/n1/full/msb4100187.html). Showed a regulatory range (ON/OFF) of 1.5x10^4.
mutable provenance: Annealed from oligos.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K137124_sequence/1

BBa_K427004
type: ComponentDefinition
persistent identity: BBa K427004
display id: BBa_K427004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Pmom promoter of Mu bacteriophage
creator: Jan Marte Contreras Ortiz
created: 2010-10-21T11:00:00Z
modified: 2015-05-08T01:12:29Z
discontinued: false
dominant: false
group u list: _544_
m user id: 0
owner id: 6602
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Pmom is one of the four late promoters of the Mu bacteriophage. It can be activated by the C protein, which is produced by the middle promoter Pm. Pmom works because of a special configuration of the DNA. It can be used to create a sensitivity tuner alongside the C protein of the same phage. This construction can be used to increase the POPS output of a promoter.
mutable notes: The design of this part had no special considerations
mutable provenance: Synthetized from the reported sequence of Pmom promoter from Mu bacteriophage
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: Pmom
sequence: https://synbiohub.org/public/igem/BBa_K427004_sequence/1

Pmom
type: SequenceAnnotation
persistent identity: annotation2092368
display id: annotation2092368
version: 1
top level: BBa_K427004
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K427004/annotation2092368/range2092368/1

https://synbiohub.org/public/igem/BBa_K427004/annotation2092368/range2092368/1
type: Range
persistent identity: range2092368
display id: range2092368
version: 1
direction: direction/forward
top level: BBa_K427004
start: 1
end: 79
orientation: inline

BBa_K136010
type: ComponentDefinition
persistent identity: BBa K136010
display id: BBa_K136010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: fliA promoter
creator: Kok-Phen YAN
created: 2008-10-24T11:00:00Z
modified: 2015-05-08T01:10:02Z
discontinued: false
dominant: false
group u list: _211_
m user id: 0
owner id: 3262
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: FliA is a transcription factor involved in the formation of the flagella in E. coli. The promotor of fliA is induced by FlhDC. FliA seems to have a positive autoregulation on its promotor.
mutable notes: primers used
mutable provenance: This part comes from the genome of E. coli K12 strain MG1655.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: FlhDC binding site 1
sequence annotation: A mutated to G
sequence annotation: transcription initiation site
sequence annotation: ATG
sequence annotation: FlhDC binding site 2
sequence annotation: natural fliA RBS
sequence: https://synbiohub.org/public/igem/BBa_K136010_sequence/1

FlhDC binding site 1
type: SequenceAnnotation
persistent identity: annotation1987040
display id: annotation1987040
version: 1
top level: BBa_K136010
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K136010/annotation1987040/range1987040/1

https://synbiohub.org/public/igem/BBa_K136010/annotation1987040/range1987040/1
type: Range
persistent identity: range1987040
display id: range1987040
version: 1
direction: direction/forward
top level: BBa_K136010
start: 198
end: 225
orientation: inline

A mutated to G
type: SequenceAnnotation
persistent identity: annotation1987043
display id: annotation1987043
version: 1
top level: BBa_K136010
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K136010/annotation1987043/range1987043/1

https://synbiohub.org/public/igem/BBa_K136010/annotation1987043/range1987043/1
type: Range
persistent identity: range1987043
display id: range1987043
version: 1
direction: direction/forward
top level: BBa_K136010
start: 316
end: 316
orientation: inline

transcription initiation site
type: SequenceAnnotation
persistent identity: annotation1987045
display id: annotation1987045
version: 1
top level: BBa_K136010
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K136010/annotation1987045/range1987045/1

https://synbiohub.org/public/igem/BBa_K136010/annotation1987045/range1987045/1
type: Range
persistent identity: range1987045
display id: range1987045
version: 1
direction: direction/forward
top level: BBa_K136010
start: 282
end: 282
orientation: inline

ATG
type: SequenceAnnotation
persistent identity: annotation1987044
display id: annotation1987044
version: 1
top level: BBa_K136010
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K136010/annotation1987044/range1987044/1

https://synbiohub.org/public/igem/BBa_K136010/annotation1987044/range1987044/1
type: Range
persistent identity: range1987044
display id: range1987044
version: 1
direction: direction/forward
top level: BBa_K136010
start: 342
end: 344
orientation: inline

FlhDC binding site 2
type: SequenceAnnotation
persistent identity: annotation1987041
display id: annotation1987041
version: 1
top level: BBa_K136010
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K136010/annotation1987041/range1987041/1

https://synbiohub.org/public/igem/BBa_K136010/annotation1987041/range1987041/1
type: Range
persistent identity: range1987041
display id: range1987041
version: 1
direction: direction/forward
top level: BBa_K136010
start: 226
end: 261
orientation: inline

A mutated to G
type: SequenceAnnotation
persistent identity: annotation1987042
display id: annotation1987042
version: 1
top level: BBa_K136010
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K136010/annotation1987042/range1987042/1

https://synbiohub.org/public/igem/BBa_K136010/annotation1987042/range1987042/1
type: Range
persistent identity: range1987042
display id: range1987042
version: 1
direction: direction/forward
top level: BBa_K136010
start: 263
end: 263
orientation: inline

transcription initiation site
type: SequenceAnnotation
persistent identity: annotation1987046
display id: annotation1987046
version: 1
top level: BBa_K136010
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K136010/annotation1987046/range1987046/1

https://synbiohub.org/public/igem/BBa_K136010/annotation1987046/range1987046/1
type: Range
persistent identity: range1987046
display id: range1987046
version: 1
direction: direction/forward
top level: BBa_K136010
start: 293
end: 293
orientation: inline

natural fliA RBS
type: SequenceAnnotation
persistent identity: annotation1999348
display id: annotation1999348
version: 1
top level: BBa_K136010
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K136010/annotation1999348/range1999348/1

https://synbiohub.org/public/igem/BBa_K136010/annotation1999348/range1999348/1
type: Range
persistent identity: range1999348
display id: range1999348
version: 1
direction: direction/forward
top level: BBa_K136010
start: 330
end: 337
orientation: inline

Pnif
type: ComponentDefinition
persistent identity: BBa K1796001
display id: BBa_K1796001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: promoter of Paenibacillus sp. WLY78 nif gene cluster
creator: Nannan Xie
created: 2015-09-16T11:00:00Z
modified: 2015-09-18T02:35:42Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2221_
m user id: 12637
owner id: 23592
owning group id: 9
status: status/Planning
bookmark: false
mutable description: The promoter of Paenibacillus sp. WLY78 nif gene cluster, We named it Pnif. Pnif is a σ70-dependent promoter located upstream of nifB. Pnif is in control of all the gene clusters of nitrogen fixation genome. Confirmation of nif promoter We standardized the nif promoter of Paenibacillus sp. WLY78, and linked it to the upstream of 3 reporter genes (RFP reporter: BBa_K1357010, YFP reporter: BBa_E0430;amilCP reporter: BBa_K1357009) to confirm the reliability of this promoter. The results are shown in Figure 2. A B C http://2015.igem.org/wiki/images/b/b2/Confirmation_of_Pnif_AB.png http://2015.igem.org/wiki/images/thumb/3/34/C-confirmation_of_Pnif.jpeg/622px-C-confirmation_of_Pnif.jpeg Fig.4. Confirmation of nif promoter of Paenibacillus sp. WLY78. (A) Pnif+RFP reporter; (B) Pnif+YFP reporter;(C)Pnif+amilCP reporter. The results confirmed the reliability of Pnif. The initiation of Pnif does not require inducement. Thus it is a effective constitutive promoter.
mutable notes: effeciency of the promoter in E.coli for ordinary parts
mutable provenance: Pnif is from Paenibacillus sp. WLY78 genome,located upstream of nifB,the first gene of nitrogen fixation cluster.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -10
sequence annotation: -35
sequence annotation: Pnif
sequence: https://synbiohub.org/public/igem/BBa_K1796001_sequence/1

-10
type: SequenceAnnotation
persistent identity: annotation2473660
display id: annotation2473660
version: 1
top level: Pnif
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K1796001/annotation2473660/range2473660/1

https://synbiohub.org/public/igem/BBa_K1796001/annotation2473660/range2473660/1
type: Range
persistent identity: range2473660
display id: range2473660
version: 1
direction: direction/forward
top level: Pnif
start: 85
end: 90
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2473637
display id: annotation2473637
version: 1
top level: Pnif
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K1796001/annotation2473637/range2473637/1

https://synbiohub.org/public/igem/BBa_K1796001/annotation2473637/range2473637/1
type: Range
persistent identity: range2473637
display id: range2473637
version: 1
direction: direction/forward
top level: Pnif
start: 62
end: 67
orientation: inline

Pnif
type: SequenceAnnotation
persistent identity: annotation2473661
display id: annotation2473661
version: 1
top level: Pnif
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1796001/annotation2473661/range2473661/1

https://synbiohub.org/public/igem/BBa_K1796001/annotation2473661/range2473661/1
type: Range
persistent identity: range2473661
display id: range2473661
version: 1
direction: direction/forward
top level: Pnif
start: 1
end: 100
orientation: inline

BBa_I741019
type: ComponentDefinition
persistent identity: BBa I741019
display id: BBa_I741019
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Right facing promoter (for xylA) controlled by xylR and CRP-cAMP
creator: Lucien Weiss
created: 2007-06-20T11:00:00Z
modified: 2015-08-31T04:08:02Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _143_
m user id: 0
owner id: 1763
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: naturally the promoter to xylA and xylB
mutable notes: removed RBS cut out several xylR activation sites
mutable provenance: ecoli k12 chromosome
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: CRP-cAMP transcriptional dual regulator activates transcription
sequence annotation: XylR-Xylose transcriptional activator activates transcription
sequence annotation: xylAp
sequence: https://synbiohub.org/public/igem/BBa_I741019_sequence/1

CRP-cAMP transcriptional dual regulator activates transcription
type: SequenceAnnotation
persistent identity: annotation1935193
display id: annotation1935193
version: 1
top level: BBa_I741019
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I741019/annotation1935193/range1935193/1

https://synbiohub.org/public/igem/BBa_I741019/annotation1935193/range1935193/1
type: Range
persistent identity: range1935193
display id: range1935193
version: 1
direction: direction/forward
top level: BBa_I741019
start: 86
end: 107
orientation: inline

XylR-Xylose transcriptional activator activates transcription
type: SequenceAnnotation
persistent identity: annotation1935211
display id: annotation1935211
version: 1
top level: BBa_I741019
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I741019/annotation1935211/range1935211/1

https://synbiohub.org/public/igem/BBa_I741019/annotation1935211/range1935211/1
type: Range
persistent identity: range1935211
display id: range1935211
version: 1
direction: direction/reverse
top level: BBa_I741019
start: 48
end: 64
orientation: inline

xylAp
type: SequenceAnnotation
persistent identity: annotation1935191
display id: annotation1935191
version: 1
top level: BBa_I741019
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I741019/annotation1935191/range1935191/1

https://synbiohub.org/public/igem/BBa_I741019/annotation1935191/range1935191/1
type: Range
persistent identity: range1935191
display id: range1935191
version: 1
direction: direction/reverse
top level: BBa_I741019
start: 76
end: 117
orientation: inline

XylR-Xylose transcriptional activator activates transcription
type: SequenceAnnotation
persistent identity: annotation1935195
display id: annotation1935195
version: 1
top level: BBa_I741019
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I741019/annotation1935195/range1935195/1

https://synbiohub.org/public/igem/BBa_I741019/annotation1935195/range1935195/1
type: Range
persistent identity: range1935195
display id: range1935195
version: 1
direction: direction/forward
top level: BBa_I741019
start: 69
end: 85
orientation: inline

BBa_K180014
type: ComponentDefinition
persistent identity: BBa K180014
display id: BBa_K180014
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Rock-paper-scissors - Paper primary plasmid
creator: Richard Wilson, Stephanie Chambers
created: 2009-10-20T11:00:00Z
modified: 2015-05-08T01:11:06Z
discontinued: false
dominant: false
experience: experience/None
group u list: _278_
m user id: 0
owner id: 4082
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: Composite part, consisting of BBa_K180005 and BBa_K180015, but with BBa_K180005 reading along the reverse strand, and BBa_K180015 reading along the forward strand. Forms the 'paper' primary plasmid in Team:Southampton's rock-paper-scissors project design.
mutable notes: BBa_K180005 reading along reverse strand, and BBa_K180015 reading along the forward strand to ensure the constitutive promoter BBa_J23119 (present in BBa_K180005) has no effect over GFP or RhlI expression in BBa_K180015.
mutable provenance: See relevant biobrick component parts (BBa_K180011 and BBa_K180012).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: K180005
sequence annotation: B0015
sequence annotation: C0170
sequence annotation: conserved
sequence annotation: -35
sequence annotation: luxR
sequence annotation: rhlI
sequence annotation: -10
sequence annotation: B0034
sequence annotation: Ptac
sequence annotation: C0062
sequence annotation: E0040
sequence annotation: K180015
sequence annotation: lacIq
sequence annotation: GFPmut3b
sequence annotation: K180000
sequence annotation: J23119
sequence: https://synbiohub.org/public/igem/BBa_K180014_sequence/1

K180005
type: SequenceAnnotation
persistent identity: annotation2058320
display id: annotation2058320
version: 1
top level: BBa_K180014
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K180014/annotation2058320/range2058320/1

https://synbiohub.org/public/igem/BBa_K180014/annotation2058320/range2058320/1
type: Range
persistent identity: range2058320
display id: range2058320
version: 1
direction: direction/reverse
top level: BBa_K180014
start: 1
end: 979
orientation: inline

B0015
type: SequenceAnnotation
persistent identity: annotation2058343
display id: annotation2058343
version: 1
top level: BBa_K180014
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K180014/annotation2058343/range2058343/1

https://synbiohub.org/public/igem/BBa_K180014/annotation2058343/range2058343/1
type: Range
persistent identity: range2058343
display id: range2058343
version: 1
direction: direction/forward
top level: BBa_K180014
start: 3824
end: 3952
orientation: inline

C0170
type: SequenceAnnotation
persistent identity: annotation2058341
display id: annotation2058341
version: 1
top level: BBa_K180014
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K180014/annotation2058341/range2058341/1

https://synbiohub.org/public/igem/BBa_K180014/annotation2058341/range2058341/1
type: Range
persistent identity: range2058341
display id: range2058341
version: 1
direction: direction/forward
top level: BBa_K180014
start: 3207
end: 3815
orientation: inline

conserved
type: SequenceAnnotation
persistent identity: annotation2058328
display id: annotation2058328
version: 1
top level: BBa_K180014
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K180014/annotation2058328/range2058328/1

https://synbiohub.org/public/igem/BBa_K180014/annotation2058328/range2058328/1
type: Range
persistent identity: range2058328
display id: range2058328
version: 1
direction: direction/forward
top level: BBa_K180014
start: 2447
end: 2450
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2058334
display id: annotation2058334
version: 1
top level: BBa_K180014
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K180014/annotation2058334/range2058334/1

https://synbiohub.org/public/igem/BBa_K180014/annotation2058334/range2058334/1
type: Range
persistent identity: range2058334
display id: range2058334
version: 1
direction: direction/reverse
top level: BBa_K180014
start: 975
end: 979
orientation: inline

luxR
type: SequenceAnnotation
persistent identity: annotation2058326
display id: annotation2058326
version: 1
top level: BBa_K180014
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K180014/annotation2058326/range2058326/1

https://synbiohub.org/public/igem/BBa_K180014/annotation2058326/range2058326/1
type: Range
persistent identity: range2058326
display id: range2058326
version: 1
direction: direction/reverse
top level: BBa_K180014
start: 169
end: 918
orientation: inline

rhlI
type: SequenceAnnotation
persistent identity: annotation2058342
display id: annotation2058342
version: 1
top level: BBa_K180014
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K180014/annotation2058342/range2058342/1

https://synbiohub.org/public/igem/BBa_K180014/annotation2058342/range2058342/1
type: Range
persistent identity: range2058342
display id: range2058342
version: 1
direction: direction/forward
top level: BBa_K180014
start: 3207
end: 3815
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2058335
display id: annotation2058335
version: 1
top level: BBa_K180014
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K180014/annotation2058335/range2058335/1

https://synbiohub.org/public/igem/BBa_K180014/annotation2058335/range2058335/1
type: Range
persistent identity: range2058335
display id: range2058335
version: 1
direction: direction/reverse
top level: BBa_K180014
start: 951
end: 956
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation2058331
display id: annotation2058331
version: 1
top level: BBa_K180014
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K180014/annotation2058331/range2058331/1

https://synbiohub.org/public/igem/BBa_K180014/annotation2058331/range2058331/1
type: Range
persistent identity: range2058331
display id: range2058331
version: 1
direction: direction/reverse
top level: BBa_K180014
start: 925
end: 936
orientation: inline

Ptac
type: SequenceAnnotation
persistent identity: annotation2058338
display id: annotation2058338
version: 1
top level: BBa_K180014
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K180014/annotation2058338/range2058338/1

https://synbiohub.org/public/igem/BBa_K180014/annotation2058338/range2058338/1
type: Range
persistent identity: range2058338
display id: range2058338
version: 1
direction: direction/forward
top level: BBa_K180014
start: 2312
end: 2339
orientation: inline

conserved
type: SequenceAnnotation
persistent identity: annotation2058330
display id: annotation2058330
version: 1
top level: BBa_K180014
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K180014/annotation2058330/range2058330/1

https://synbiohub.org/public/igem/BBa_K180014/annotation2058330/range2058330/1
type: Range
persistent identity: range2058330
display id: range2058330
version: 1
direction: direction/forward
top level: BBa_K180014
start: 3193
end: 3196
orientation: inline

C0062
type: SequenceAnnotation
persistent identity: annotation2058325
display id: annotation2058325
version: 1
top level: BBa_K180014
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K180014/annotation2058325/range2058325/1

https://synbiohub.org/public/igem/BBa_K180014/annotation2058325/range2058325/1
type: Range
persistent identity: range2058325
display id: range2058325
version: 1
direction: direction/reverse
top level: BBa_K180014
start: 163
end: 918
orientation: inline

E0040
type: SequenceAnnotation
persistent identity: annotation2058339
display id: annotation2058339
version: 1
top level: BBa_K180014
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K180014/annotation2058339/range2058339/1

https://synbiohub.org/public/igem/BBa_K180014/annotation2058339/range2058339/1
type: Range
persistent identity: range2058339
display id: range2058339
version: 1
direction: direction/forward
top level: BBa_K180014
start: 2461
end: 3180
orientation: inline

K180015
type: SequenceAnnotation
persistent identity: annotation2058321
display id: annotation2058321
version: 1
top level: BBa_K180014
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K180014/annotation2058321/range2058321/1

https://synbiohub.org/public/igem/BBa_K180014/annotation2058321/range2058321/1
type: Range
persistent identity: range2058321
display id: range2058321
version: 1
direction: direction/forward
top level: BBa_K180014
start: 988
end: 3952
orientation: inline

lacIq
type: SequenceAnnotation
persistent identity: annotation2058337
display id: annotation2058337
version: 1
top level: BBa_K180014
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K180014/annotation2058337/range2058337/1

https://synbiohub.org/public/igem/BBa_K180014/annotation2058337/range2058337/1
type: Range
persistent identity: range2058337
display id: range2058337
version: 1
direction: direction/forward
top level: BBa_K180014
start: 988
end: 2069
orientation: inline

conserved
type: SequenceAnnotation
persistent identity: annotation2058332
display id: annotation2058332
version: 1
top level: BBa_K180014
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K180014/annotation2058332/range2058332/1

https://synbiohub.org/public/igem/BBa_K180014/annotation2058332/range2058332/1
type: Range
persistent identity: range2058332
display id: range2058332
version: 1
direction: direction/reverse
top level: BBa_K180014
start: 929
end: 932
orientation: inline

GFPmut3b
type: SequenceAnnotation
persistent identity: annotation2058340
display id: annotation2058340
version: 1
top level: BBa_K180014
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K180014/annotation2058340/range2058340/1

https://synbiohub.org/public/igem/BBa_K180014/annotation2058340/range2058340/1
type: Range
persistent identity: range2058340
display id: range2058340
version: 1
direction: direction/forward
top level: BBa_K180014
start: 2461
end: 3180
orientation: inline

K180000
type: SequenceAnnotation
persistent identity: annotation2058336
display id: annotation2058336
version: 1
top level: BBa_K180014
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K180014/annotation2058336/range2058336/1

https://synbiohub.org/public/igem/BBa_K180014/annotation2058336/range2058336/1
type: Range
persistent identity: range2058336
display id: range2058336
version: 1
direction: direction/forward
top level: BBa_K180014
start: 988
end: 2434
orientation: inline

J23119
type: SequenceAnnotation
persistent identity: annotation2058333
display id: annotation2058333
version: 1
top level: BBa_K180014
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K180014/annotation2058333/range2058333/1

https://synbiohub.org/public/igem/BBa_K180014/annotation2058333/range2058333/1
type: Range
persistent identity: range2058333
display id: range2058333
version: 1
direction: direction/reverse
top level: BBa_K180014
start: 945
end: 979
orientation: inline

B0015
type: SequenceAnnotation
persistent identity: annotation2058324
display id: annotation2058324
version: 1
top level: BBa_K180014
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K180014/annotation2058324/range2058324/1

https://synbiohub.org/public/igem/BBa_K180014/annotation2058324/range2058324/1
type: Range
persistent identity: range2058324
display id: range2058324
version: 1
direction: direction/reverse
top level: BBa_K180014
start: 1
end: 129
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation2058327
display id: annotation2058327
version: 1
top level: BBa_K180014
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K180014/annotation2058327/range2058327/1

https://synbiohub.org/public/igem/BBa_K180014/annotation2058327/range2058327/1
type: Range
persistent identity: range2058327
display id: range2058327
version: 1
direction: direction/forward
top level: BBa_K180014
start: 2443
end: 2454
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation2058329
display id: annotation2058329
version: 1
top level: BBa_K180014
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K180014/annotation2058329/range2058329/1

https://synbiohub.org/public/igem/BBa_K180014/annotation2058329/range2058329/1
type: Range
persistent identity: range2058329
display id: range2058329
version: 1
direction: direction/forward
top level: BBa_K180014
start: 3189
end: 3200
orientation: inline

pRE
type: ComponentDefinition
persistent identity: BBa K116603
display id: BBa_K116603
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pRE promoter from λ phage
creator: Jesse Wu
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:09:33Z
discontinued: false
dominant: false
experience: experience/None
group u list: _210_
m user id: 0
owner id: 2749
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: The pRE coding region from λ phage. It is able to be induced by CII (BBa_K116602).
mutable notes: none.
mutable provenance: λ phage
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K116603_sequence/1

BBa_I714924
type: ComponentDefinition
persistent identity: BBa I714924
display id: BBa_I714924
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RecA_DlexO_DLacO1
creator: Chunbo Lou
created: 2007-10-21T11:00:00Z
modified: 2015-08-31T04:07:48Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _142_
m user id: 0
owner id: 1911
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: a NOR gate
mutable notes: NOR gat
mutable provenance: synthetic
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: LexA
sequence annotation: -35
sequence annotation: -10
sequence annotation: LacO1
sequence annotation: SDY_EGFP
sequence: https://synbiohub.org/public/igem/BBa_I714924_sequence/1

LexA
type: SequenceAnnotation
persistent identity: annotation1951922
display id: annotation1951922
version: 1
top level: BBa_I714924
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I714924/annotation1951922/range1951922/1

https://synbiohub.org/public/igem/BBa_I714924/annotation1951922/range1951922/1
type: Range
persistent identity: range1951922
display id: range1951922
version: 1
direction: direction/forward
top level: BBa_I714924
start: 65
end: 80
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1951925
display id: annotation1951925
version: 1
top level: BBa_I714924
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I714924/annotation1951925/range1951925/1

https://synbiohub.org/public/igem/BBa_I714924/annotation1951925/range1951925/1
type: Range
persistent identity: range1951925
display id: range1951925
version: 1
direction: direction/forward
top level: BBa_I714924
start: 59
end: 64
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1951926
display id: annotation1951926
version: 1
top level: BBa_I714924
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I714924/annotation1951926/range1951926/1

https://synbiohub.org/public/igem/BBa_I714924/annotation1951926/range1951926/1
type: Range
persistent identity: range1951926
display id: range1951926
version: 1
direction: direction/forward
top level: BBa_I714924
start: 81
end: 86
orientation: inline

LexA
type: SequenceAnnotation
persistent identity: annotation1956155
display id: annotation1956155
version: 1
top level: BBa_I714924
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I714924/annotation1956155/range1956155/1

https://synbiohub.org/public/igem/BBa_I714924/annotation1956155/range1956155/1
type: Range
persistent identity: range1956155
display id: range1956155
version: 1
direction: direction/forward
top level: BBa_I714924
start: 35
end: 55
orientation: inline

LacO1
type: SequenceAnnotation
persistent identity: annotation1951924
display id: annotation1951924
version: 1
top level: BBa_I714924
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I714924/annotation1951924/range1951924/1

https://synbiohub.org/public/igem/BBa_I714924/annotation1951924/range1951924/1
type: Range
persistent identity: range1951924
display id: range1951924
version: 1
direction: direction/forward
top level: BBa_I714924
start: 93
end: 112
orientation: inline

LacO1
type: SequenceAnnotation
persistent identity: annotation1951921
display id: annotation1951921
version: 1
top level: BBa_I714924
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I714924/annotation1951921/range1951921/1

https://synbiohub.org/public/igem/BBa_I714924/annotation1951921/range1951921/1
type: Range
persistent identity: range1951921
display id: range1951921
version: 1
direction: direction/forward
top level: BBa_I714924
start: 1
end: 21
orientation: inline

SDY_EGFP
type: SequenceAnnotation
persistent identity: annotation1956422
display id: annotation1956422
version: 1
top level: BBa_I714924
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_I714924/annotation1956422/range1956422/1

https://synbiohub.org/public/igem/BBa_I714924/annotation1956422/range1956422/1
type: Range
persistent identity: range1956422
display id: range1956422
version: 1
direction: direction/forward
top level: BBa_I714924
start: 121
end: 862
orientation: inline

BBa_J06403
type: ComponentDefinition
persistent identity: BBa J06403
display id: BBa_J06403
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RhIR promoter repressible by CI
creator: pbradley
created: 2005-06-23T11:00:00Z
modified: 2015-08-31T04:08:17Z
discontinued: false
dominant: false
experience: experience/None
group u list: _20_
m user id: 0
owner id: 358
owning group id: 20
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: THIS PART DOES NOT WORK. Need to add the CI operator sites.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -35
sequence annotation: modified P(Rhl)
sequence annotation: RhlR
sequence annotation: BBa_I14017
sequence: https://synbiohub.org/public/igem/BBa_J06403_sequence/1

-35
type: SequenceAnnotation
persistent identity: annotation1538367
display id: annotation1538367
version: 1
top level: BBa_J06403
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J06403/annotation1538367/range1538367/1

https://synbiohub.org/public/igem/BBa_J06403/annotation1538367/range1538367/1
type: Range
persistent identity: range1538367
display id: range1538367
version: 1
direction: direction/forward
top level: BBa_J06403
start: 21
end: 50
orientation: inline

modified P(Rhl)
type: SequenceAnnotation
persistent identity: annotation1538365
display id: annotation1538365
version: 1
top level: BBa_J06403
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_J06403/annotation1538365/range1538365/1

https://synbiohub.org/public/igem/BBa_J06403/annotation1538365/range1538365/1
type: Range
persistent identity: range1538365
display id: range1538365
version: 1
direction: direction/forward
top level: BBa_J06403
start: 1
end: 51
orientation: inline

RhlR
type: SequenceAnnotation
persistent identity: annotation1538366
display id: annotation1538366
version: 1
top level: BBa_J06403
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_J06403/annotation1538366/range1538366/1

https://synbiohub.org/public/igem/BBa_J06403/annotation1538366/range1538366/1
type: Range
persistent identity: range1538366
display id: range1538366
version: 1
direction: direction/forward
top level: BBa_J06403
start: 1
end: 20
orientation: inline

BBa_I14017
type: SequenceAnnotation
persistent identity: annotation1538368
display id: annotation1538368
version: 1
top level: BBa_J06403
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_J06403/annotation1538368/range1538368/1

https://synbiohub.org/public/igem/BBa_J06403/annotation1538368/range1538368/1
type: Range
persistent identity: range1538368
display id: range1538368
version: 1
direction: direction/forward
top level: BBa_J06403
start: 1
end: 51
orientation: inline

BBa_K112406
type: ComponentDefinition
persistent identity: BBa K112406
display id: BBa_K112406
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: cadC promoter
creator: Bing Xia
created: 2008-10-21T11:00:00Z
modified: 2015-05-08T01:09:17Z
discontinued: false
dominant: false
group u list: _224_
m user id: 0
owner id: 2998
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: ---- This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at: [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
mutable provenance: Biobricking of cadC promoter and cadC gene PCR cadC-F and cadC-R on MG1655 (2336 bp, EcoR1/BamH1) Sub into pBca1256 (EcoR1/BamH1 910+2472bp, L) Product is pBca1256-K112406 ---------------------------- cb10013-cadC-F cgataGAATTCatgAGATCTcaccagcaatttgcgggcatatc cb10012-cad-R cgtagGGATCCtgaccagaccaaactggcg
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K112406_sequence/1

BBa_K1520516
type: ComponentDefinition
persistent identity: BBa K1520516
display id: BBa_K1520516
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: MC31-rbs-luxI-Ter-Pcons2-rbs-luxR-Ter-Prlux-rbs-OMPA-golB-rbs-luxI-Ter
creator: yue wu
created: 2014-09-22T11:00:00Z
modified: 2015-05-08T01:10:49Z
discontinued: false
dominant: false
group u list: _1902_
m user id: 0
owner id: 21318
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: MC31-rbs-luxI-Ter-Pcons2-rbs-luxR-Ter-Prlux-rbs-OMPA-golB-rbs-luxI-Ter It is a test of our positive feedback circuit for microcystin.
mutable notes: None
mutable provenance: MC31, luxI, Pcons2, luxR, Prlux, OMPA-golB, Ter
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1520003
component: BBa_K1520002
component: BBa_K1520504
component: BBa_K1520507
component: BBa_R0062
component: BBa_K1520029
sequence annotation: BBa_K1520002
sequence annotation: BBa_K1520504
sequence annotation: BBa_R0062
sequence annotation: BBa_K1520029
sequence annotation: BBa_K1520507
sequence annotation: BBa_K1520003
sequence: https://synbiohub.org/public/igem/BBa_K1520516_sequence/1

BBa_K1520003
type: Component
persistent identity: component2385702
display id: component2385702
version: 1
top level: BBa_K1520516
access: public
definition: https://synbiohub.org/public/igem/BBa_K1520003/1

BBa_K1520002
type: Component
persistent identity: component2385692
display id: component2385692
version: 1
top level: BBa_K1520516
access: public
definition: https://synbiohub.org/public/igem/BBa_K1520002/1

BBa_K1520504
type: Component
persistent identity: component2385695
display id: component2385695
version: 1
top level: BBa_K1520516
access: public
definition: https://synbiohub.org/public/igem/BBa_K1520504/1

BBa_K1520507
type: Component
persistent identity: component2385714
display id: component2385714
version: 1
top level: BBa_K1520516
access: public
definition: https://synbiohub.org/public/igem/BBa_K1520507/1

BBa_R0062
type: Component
persistent identity: component2385704
display id: component2385704
version: 1
top level: BBa_K1520516
access: public
definition: lux pR

BBa_K1520504
type: Component
persistent identity: component2385717
display id: component2385717
version: 1
top level: BBa_K1520516
access: public
definition: https://synbiohub.org/public/igem/BBa_K1520504/1

BBa_K1520029
type: Component
persistent identity: component2385718
display id: component2385718
version: 1
top level: BBa_K1520516
access: public
definition: https://synbiohub.org/public/igem/BBa_K1520029/1

BBa_K1520029
type: Component
persistent identity: component2385696
display id: component2385696
version: 1
top level: BBa_K1520516
access: public
definition: https://synbiohub.org/public/igem/BBa_K1520029/1

BBa_K1520002
type: SequenceAnnotation
persistent identity: annotation2385692
display id: annotation2385692
version: 1
top level: BBa_K1520516
location: https://synbiohub.org/public/igem/BBa_K1520516/annotation2385692/range2385692/1
component: BBa_K1520002

https://synbiohub.org/public/igem/BBa_K1520516/annotation2385692/range2385692/1
type: Range
persistent identity: range2385692
display id: range2385692
version: 1
direction: direction/reverse
top level: BBa_K1520516
start: 1
end: 132
orientation: inline

BBa_K1520504
type: SequenceAnnotation
persistent identity: annotation2385695
display id: annotation2385695
version: 1
top level: BBa_K1520516
location: https://synbiohub.org/public/igem/BBa_K1520516/annotation2385695/range2385695/1
component: BBa_K1520504

https://synbiohub.org/public/igem/BBa_K1520516/annotation2385695/range2385695/1
type: Range
persistent identity: range2385695
display id: range2385695
version: 1
direction: direction/reverse
top level: BBa_K1520516
start: 141
end: 801
orientation: inline

BBa_R0062
type: SequenceAnnotation
persistent identity: annotation2385704
display id: annotation2385704
version: 1
top level: BBa_K1520516
location: https://synbiohub.org/public/igem/BBa_K1520516/annotation2385704/range2385704/1
component: BBa_R0062

https://synbiohub.org/public/igem/BBa_K1520516/annotation2385704/range2385704/1
type: Range
persistent identity: range2385704
display id: range2385704
version: 1
direction: direction/forward
top level: BBa_K1520516
start: 1934
end: 1988
orientation: inline

BBa_K1520029
type: SequenceAnnotation
persistent identity: annotation2385718
display id: annotation2385718
version: 1
top level: BBa_K1520516
location: https://synbiohub.org/public/igem/BBa_K1520516/annotation2385718/range2385718/1
component: BBa_K1520029

https://synbiohub.org/public/igem/BBa_K1520516/annotation2385718/range2385718/1
type: Range
persistent identity: range2385718
display id: range2385718
version: 1
direction: direction/reverse
top level: BBa_K1520516
start: 3482
end: 3610
orientation: inline

BBa_K1520504
type: SequenceAnnotation
persistent identity: annotation2385717
display id: annotation2385717
version: 1
top level: BBa_K1520516
location: https://synbiohub.org/public/igem/BBa_K1520516/annotation2385717/range2385717/1
component: BBa_K1520504

https://synbiohub.org/public/igem/BBa_K1520516/annotation2385717/range2385717/1
type: Range
persistent identity: range2385717
display id: range2385717
version: 1
direction: direction/reverse
top level: BBa_K1520516
start: 2813
end: 3473
orientation: inline

BBa_K1520507
type: SequenceAnnotation
persistent identity: annotation2385714
display id: annotation2385714
version: 1
top level: BBa_K1520516
location: https://synbiohub.org/public/igem/BBa_K1520516/annotation2385714/range2385714/1
component: BBa_K1520507

https://synbiohub.org/public/igem/BBa_K1520516/annotation2385714/range2385714/1
type: Range
persistent identity: range2385714
display id: range2385714
version: 1
direction: direction/reverse
top level: BBa_K1520516
start: 1995
end: 2804
orientation: inline

BBa_K1520003
type: SequenceAnnotation
persistent identity: annotation2385702
display id: annotation2385702
version: 1
top level: BBa_K1520516
location: https://synbiohub.org/public/igem/BBa_K1520516/annotation2385702/range2385702/1
component: BBa_K1520003

https://synbiohub.org/public/igem/BBa_K1520516/annotation2385702/range2385702/1
type: Range
persistent identity: range2385702
display id: range2385702
version: 1
direction: direction/reverse
top level: BBa_K1520516
start: 947
end: 1925
orientation: inline

BBa_K1520029
type: SequenceAnnotation
persistent identity: annotation2385696
display id: annotation2385696
version: 1
top level: BBa_K1520516
location: https://synbiohub.org/public/igem/BBa_K1520516/annotation2385696/range2385696/1
component: BBa_K1520029

https://synbiohub.org/public/igem/BBa_K1520516/annotation2385696/range2385696/1
type: Range
persistent identity: range2385696
display id: range2385696
version: 1
direction: direction/reverse
top level: BBa_K1520516
start: 810
end: 938
orientation: inline

BBa_I732276
type: ComponentDefinition
persistent identity: BBa I732276
display id: BBa_I732276
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member with Hybrid Operator (D001O27)
creator: Ma Rui
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:57Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732276_sequence/1

U073O11+D0
type: ComponentDefinition
persistent identity: BBa I732426
display id: BBa_I732426
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member (U073O11+D014O22)
creator: Zhan Jian
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732426_sequence/1

BBa_I715003
type: ComponentDefinition
persistent identity: BBa I715003
display id: BBa_I715003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: hybrid pLac with UV5 mutation
creator: Michael Waters
created: 2007-07-02T11:00:00Z
modified: 2015-08-31T04:07:49Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _120_
m user id: 0
owner id: 1761
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This promoter sequence is a derivative of part BBa_R0011 which is a -35 and -10 recognition site with two lac operator 1's inserted in the spacer region of the RNAP recognition region and before the -35 RANP recognition region. This part utilizes the UV5 double mutation at the -10 RNAP recognition site to recruit RNAP without the help of a CAP binding region.
mutable notes: The length of the fragment makes it cumbersome to gel purify
mutable provenance: E.Coli genome
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: lac 01
sequence annotation: -35 RNAP binding
sequence annotation: UV5 -10 RNAP recognition region double mutation
sequence annotation: -10 RNAP binding
sequence: https://synbiohub.org/public/igem/BBa_I715003_sequence/1

lac 01
type: SequenceAnnotation
persistent identity: annotation1936229
display id: annotation1936229
version: 1
top level: BBa_I715003
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_I715003/annotation1936229/range1936229/1

https://synbiohub.org/public/igem/BBa_I715003/annotation1936229/range1936229/1
type: Range
persistent identity: range1936229
display id: range1936229
version: 1
direction: direction/forward
top level: BBa_I715003
start: 3
end: 19
orientation: inline

-35 RNAP binding
type: SequenceAnnotation
persistent identity: annotation1936228
display id: annotation1936228
version: 1
top level: BBa_I715003
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I715003/annotation1936228/range1936228/1

https://synbiohub.org/public/igem/BBa_I715003/annotation1936228/range1936228/1
type: Range
persistent identity: range1936228
display id: range1936228
version: 1
direction: direction/forward
top level: BBa_I715003
start: 20
end: 25
orientation: inline

UV5 -10 RNAP recognition region double mutation
type: SequenceAnnotation
persistent identity: annotation1936232
display id: annotation1936232
version: 1
top level: BBa_I715003
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_I715003/annotation1936232/range1936232/1

https://synbiohub.org/public/igem/BBa_I715003/annotation1936232/range1936232/1
type: Range
persistent identity: range1936232
display id: range1936232
version: 1
direction: direction/forward
top level: BBa_I715003
start: 43
end: 47
orientation: inline

-10 RNAP binding
type: SequenceAnnotation
persistent identity: annotation1936231
display id: annotation1936231
version: 1
top level: BBa_I715003
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I715003/annotation1936231/range1936231/1

https://synbiohub.org/public/igem/BBa_I715003/annotation1936231/range1936231/1
type: Range
persistent identity: range1936231
display id: range1936231
version: 1
direction: direction/forward
top level: BBa_I715003
start: 43
end: 47
orientation: inline

lac 01
type: SequenceAnnotation
persistent identity: annotation1936230
display id: annotation1936230
version: 1
top level: BBa_I715003
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_I715003/annotation1936230/range1936230/1

https://synbiohub.org/public/igem/BBa_I715003/annotation1936230/range1936230/1
type: Range
persistent identity: range1936230
display id: range1936230
version: 1
direction: direction/forward
top level: BBa_I715003
start: 26
end: 42
orientation: inline

BBa_J64067
type: ComponentDefinition
persistent identity: BBa J64067
display id: BBa_J64067
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LuxR+3OC6HSL independent R0065
creator: Jeffrey J Tabor
created: 2007-12-12T12:00:00Z
modified: 2015-05-08T01:08:17Z
discontinued: false
dominant: false
experience: experience/None
group u list: _98_
m user id: 0
owner id: 88
owning group id: 98
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: The -35 box of R0065 has been modified to promote constitutive (LuxR+3OC6HSL-independent) gene expression. An extended -10 sequence has also been added to increase absolute transcription. The promoter still contains the operators OR1 and OR2 such that it retains its sensitivity to CI.
mutable notes: see above
mutable provenance: modified from R0065
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: OR1
sequence annotation: extended -10
sequence annotation: OR2
sequence annotation: lux box
sequence annotation: -35
sequence annotation: +1
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_J64067_sequence/1

OR1
type: SequenceAnnotation
persistent identity: annotation1959024
display id: annotation1959024
version: 1
top level: BBa_J64067
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J64067/annotation1959024/range1959024/1

https://synbiohub.org/public/igem/BBa_J64067/annotation1959024/range1959024/1
type: Range
persistent identity: range1959024
display id: range1959024
version: 1
direction: direction/forward
top level: BBa_J64067
start: 82
end: 98
orientation: inline

extended -10
type: SequenceAnnotation
persistent identity: annotation1959019
display id: annotation1959019
version: 1
top level: BBa_J64067
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J64067/annotation1959019/range1959019/1

https://synbiohub.org/public/igem/BBa_J64067/annotation1959019/range1959019/1
type: Range
persistent identity: range1959019
display id: range1959019
version: 1
direction: direction/forward
top level: BBa_J64067
start: 43
end: 46
orientation: inline

OR2
type: SequenceAnnotation
persistent identity: annotation1959023
display id: annotation1959023
version: 1
top level: BBa_J64067
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_J64067/annotation1959023/range1959023/1

https://synbiohub.org/public/igem/BBa_J64067/annotation1959023/range1959023/1
type: Range
persistent identity: range1959023
display id: range1959023
version: 1
direction: direction/forward
top level: BBa_J64067
start: 58
end: 75
orientation: inline

lux box
type: SequenceAnnotation
persistent identity: annotation1959021
display id: annotation1959021
version: 1
top level: BBa_J64067
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_J64067/annotation1959021/range1959021/1

https://synbiohub.org/public/igem/BBa_J64067/annotation1959021/range1959021/1
type: Range
persistent identity: range1959021
display id: range1959021
version: 1
direction: direction/forward
top level: BBa_J64067
start: 6
end: 26
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1959018
display id: annotation1959018
version: 1
top level: BBa_J64067
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J64067/annotation1959018/range1959018/1

https://synbiohub.org/public/igem/BBa_J64067/annotation1959018/range1959018/1
type: Range
persistent identity: range1959018
display id: range1959018
version: 1
direction: direction/forward
top level: BBa_J64067
start: 25
end: 30
orientation: inline

+1
type: SequenceAnnotation
persistent identity: annotation1959022
display id: annotation1959022
version: 1
top level: BBa_J64067
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J64067/annotation1959022/range1959022/1

https://synbiohub.org/public/igem/BBa_J64067/annotation1959022/range1959022/1
type: Range
persistent identity: range1959022
display id: range1959022
version: 1
direction: direction/forward
top level: BBa_J64067
start: 59
end: 59
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1959020
display id: annotation1959020
version: 1
top level: BBa_J64067
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J64067/annotation1959020/range1959020/1

https://synbiohub.org/public/igem/BBa_J64067/annotation1959020/range1959020/1
type: Range
persistent identity: range1959020
display id: range1959020
version: 1
direction: direction/forward
top level: BBa_J64067
start: 48
end: 53
orientation: inline

U097NUL+D0
type: ComponentDefinition
persistent identity: BBa I732408
display id: BBa_I732408
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member (U097NUL+D014O22)
creator: Zhan Jian
created: 2007-09-19T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1572
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: A Double Reporter (LacZ-alpha and RFP) loaded on pSB3C5. For more information please refer to BBa_I732402.
mutable notes: Might be later edited.
mutable provenance: Might be later edited.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: lacZ
sequence annotation: BBa_B0034
sequence annotation: T7 TE
sequence annotation: mrfp1
sequence annotation: conserved
sequence annotation: stem_loop
sequence annotation: BglII
sequence annotation: STOP
sequence annotation: BBa_B0012
sequence annotation: BBa_I732006
sequence annotation: barcode
sequence annotation: START
sequence annotation: BBa_I732007
sequence annotation: stop
sequence annotation: BBa_B0010
sequence annotation: polya
sequence annotation: BamHI
sequence annotation: BBa_E1010
sequence: https://synbiohub.org/public/igem/BBa_I732408_sequence/1

lacZ
type: SequenceAnnotation
persistent identity: annotation1944503
display id: annotation1944503
version: 1
top level: U097NUL+D0
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_I732408/annotation1944503/range1944503/1

https://synbiohub.org/public/igem/BBa_I732408/annotation1944503/range1944503/1
type: Range
persistent identity: range1944503
display id: range1944503
version: 1
direction: direction/forward
top level: U097NUL+D0
start: 46
end: 279
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1944507
display id: annotation1944507
version: 1
top level: U097NUL+D0
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I732408/annotation1944507/range1944507/1

https://synbiohub.org/public/igem/BBa_I732408/annotation1944507/range1944507/1
type: Range
persistent identity: range1944507
display id: range1944507
version: 1
direction: direction/reverse
top level: U097NUL+D0
start: 288
end: 299
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1944514
display id: annotation1944514
version: 1
top level: U097NUL+D0
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_I732408/annotation1944514/range1944514/1

https://synbiohub.org/public/igem/BBa_I732408/annotation1944514/range1944514/1
type: Range
persistent identity: range1944514
display id: range1944514
version: 1
direction: direction/forward
top level: U097NUL+D0
start: 1115
end: 1134
orientation: inline

mrfp1
type: SequenceAnnotation
persistent identity: annotation1944508
display id: annotation1944508
version: 1
top level: U097NUL+D0
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_I732408/annotation1944508/range1944508/1

https://synbiohub.org/public/igem/BBa_I732408/annotation1944508/range1944508/1
type: Range
persistent identity: range1944508
display id: range1944508
version: 1
direction: direction/forward
top level: U097NUL+D0
start: 306
end: 980
orientation: inline

conserved
type: SequenceAnnotation
persistent identity: annotation1944506
display id: annotation1944506
version: 1
top level: U097NUL+D0
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_I732408/annotation1944506/range1944506/1

https://synbiohub.org/public/igem/BBa_I732408/annotation1944506/range1944506/1
type: Range
persistent identity: range1944506
display id: range1944506
version: 1
direction: direction/forward
top level: U097NUL+D0
start: 292
end: 295
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation1944512
display id: annotation1944512
version: 1
top level: U097NUL+D0
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_I732408/annotation1944512/range1944512/1

https://synbiohub.org/public/igem/BBa_I732408/annotation1944512/range1944512/1
type: Range
persistent identity: range1944512
display id: range1944512
version: 1
direction: direction/forward
top level: U097NUL+D0
start: 1031
end: 1074
orientation: inline

BglII
type: SequenceAnnotation
persistent identity: annotation1944497
display id: annotation1944497
version: 1
top level: U097NUL+D0
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I732408/annotation1944497/range1944497/1

https://synbiohub.org/public/igem/BBa_I732408/annotation1944497/range1944497/1
type: Range
persistent identity: range1944497
display id: range1944497
version: 1
direction: direction/forward
top level: U097NUL+D0
start: 3
end: 8
orientation: inline

conserved
type: SequenceAnnotation
persistent identity: annotation1944500
display id: annotation1944500
version: 1
top level: U097NUL+D0
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_I732408/annotation1944500/range1944500/1

https://synbiohub.org/public/igem/BBa_I732408/annotation1944500/range1944500/1
type: Range
persistent identity: range1944500
display id: range1944500
version: 1
direction: direction/forward
top level: U097NUL+D0
start: 32
end: 35
orientation: inline

STOP
type: SequenceAnnotation
persistent identity: annotation1944504
display id: annotation1944504
version: 1
top level: U097NUL+D0
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_I732408/annotation1944504/range1944504/1

https://synbiohub.org/public/igem/BBa_I732408/annotation1944504/range1944504/1
type: Range
persistent identity: range1944504
display id: range1944504
version: 1
direction: direction/forward
top level: U097NUL+D0
start: 274
end: 279
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1944513
display id: annotation1944513
version: 1
top level: U097NUL+D0
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I732408/annotation1944513/range1944513/1

https://synbiohub.org/public/igem/BBa_I732408/annotation1944513/range1944513/1
type: Range
persistent identity: range1944513
display id: range1944513
version: 1
direction: direction/forward
top level: U097NUL+D0
start: 1108
end: 1148
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1944501
display id: annotation1944501
version: 1
top level: U097NUL+D0
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I732408/annotation1944501/range1944501/1

https://synbiohub.org/public/igem/BBa_I732408/annotation1944501/range1944501/1
type: Range
persistent identity: range1944501
display id: range1944501
version: 1
direction: direction/reverse
top level: U097NUL+D0
start: 28
end: 39
orientation: inline

BBa_I732006
type: SequenceAnnotation
persistent identity: annotation1944505
display id: annotation1944505
version: 1
top level: U097NUL+D0
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I732408/annotation1944505/range1944505/1

https://synbiohub.org/public/igem/BBa_I732408/annotation1944505/range1944505/1
type: Range
persistent identity: range1944505
display id: range1944505
version: 1
direction: direction/reverse
top level: U097NUL+D0
start: 46
end: 279
orientation: inline

barcode
type: SequenceAnnotation
persistent identity: annotation1944510
display id: annotation1944510
version: 1
top level: U097NUL+D0
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I732408/annotation1944510/range1944510/1

https://synbiohub.org/public/igem/BBa_I732408/annotation1944510/range1944510/1
type: Range
persistent identity: range1944510
display id: range1944510
version: 1
direction: direction/reverse
top level: U097NUL+D0
start: 987
end: 1011
orientation: inline

START
type: SequenceAnnotation
persistent identity: annotation1944502
display id: annotation1944502
version: 1
top level: U097NUL+D0
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_I732408/annotation1944502/range1944502/1

https://synbiohub.org/public/igem/BBa_I732408/annotation1944502/range1944502/1
type: Range
persistent identity: range1944502
display id: range1944502
version: 1
direction: direction/forward
top level: U097NUL+D0
start: 46
end: 48
orientation: inline

BBa_I732007
type: SequenceAnnotation
persistent identity: annotation1944499
display id: annotation1944499
version: 1
top level: U097NUL+D0
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I732408/annotation1944499/range1944499/1

https://synbiohub.org/public/igem/BBa_I732408/annotation1944499/range1944499/1
type: Range
persistent identity: range1944499
display id: range1944499
version: 1
direction: direction/reverse
top level: U097NUL+D0
start: 1
end: 19
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1944516
display id: annotation1944516
version: 1
top level: U097NUL+D0
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_I732408/annotation1944516/range1944516/1

https://synbiohub.org/public/igem/BBa_I732408/annotation1944516/range1944516/1
type: Range
persistent identity: range1944516
display id: range1944516
version: 1
direction: direction/forward
top level: U097NUL+D0
start: 1141
end: 1141
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1944511
display id: annotation1944511
version: 1
top level: U097NUL+D0
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I732408/annotation1944511/range1944511/1

https://synbiohub.org/public/igem/BBa_I732408/annotation1944511/range1944511/1
type: Range
persistent identity: range1944511
display id: range1944511
version: 1
direction: direction/forward
top level: U097NUL+D0
start: 1020
end: 1099
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1944515
display id: annotation1944515
version: 1
top level: U097NUL+D0
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_I732408/annotation1944515/range1944515/1

https://synbiohub.org/public/igem/BBa_I732408/annotation1944515/range1944515/1
type: Range
persistent identity: range1944515
display id: range1944515
version: 1
direction: direction/forward
top level: U097NUL+D0
start: 1135
end: 1148
orientation: inline

BamHI
type: SequenceAnnotation
persistent identity: annotation1944498
display id: annotation1944498
version: 1
top level: U097NUL+D0
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I732408/annotation1944498/range1944498/1

https://synbiohub.org/public/igem/BBa_I732408/annotation1944498/range1944498/1
type: Range
persistent identity: range1944498
display id: range1944498
version: 1
direction: direction/forward
top level: U097NUL+D0
start: 12
end: 17
orientation: inline

BBa_E1010
type: SequenceAnnotation
persistent identity: annotation1944509
display id: annotation1944509
version: 1
top level: U097NUL+D0
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I732408/annotation1944509/range1944509/1

https://synbiohub.org/public/igem/BBa_I732408/annotation1944509/range1944509/1
type: Range
persistent identity: range1944509
display id: range1944509
version: 1
direction: direction/reverse
top level: U097NUL+D0
start: 306
end: 986
orientation: inline

BBa_J61054
type: ComponentDefinition
persistent identity: BBa J61054
display id: BBa_J61054
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: [HIP-1] Promoter
creator: John Anderson
created: 2007-02-20T12:00:00Z
modified: 2015-08-31T02:03:00Z
discontinued: false
dominant: false
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: non
mutable notes: non
mutable provenance: non
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J61054_sequence/1

Prm+
type: ComponentDefinition
persistent identity: BBa I12001
display id: BBa_I12001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter (PRM+)
creator: mcnamara
created: 2004-07-11T11:00:00Z
modified: 2015-08-31T04:07:31Z
discontinued: false
dominant: false
experience: experience/None
group u list: _3_
m user id: 0
owner id: 147
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I12001_sequence/1

BBa_K180017
type: ComponentDefinition
persistent identity: BBa K180017
display id: BBa_K180017
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Rock-paper-scissors - Scissors primary plasmid
creator: Richard Wilson, Stephanie Chambers
created: 2009-10-20T11:00:00Z
modified: 2015-05-08T01:11:06Z
discontinued: false
dominant: false
experience: experience/None
group u list: _278_
m user id: 0
owner id: 4082
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: Composite part, consisting of BBa_K180018 and BBa_K180019, but with BBa_K180018 reading along the reverse strand, and BBa_K180019 reading along the forward strand. Forms the 'scissors' primary plasmid in Team:Southampton's rock-paper-scissors project design.
mutable notes: BBa_K180018 reading along reverse strand, and BBa_K180019 reading along the forward strand to ensure the constitutive promoter BBa_J23119 (present in BBa_K180018) has no effect over mBanana or LuxI expression in BBa_K180019.
mutable provenance: See relevant biobrick component parts (BBa_K180018 and BBa_K180019).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: K180019
sequence annotation: mBanana
sequence annotation: conserved
sequence annotation: C0161
sequence annotation: J23119
sequence annotation: K180018
sequence annotation: Ptac
sequence annotation: lacI
sequence annotation: B0034
sequence annotation: lasR
sequence annotation: B0015
sequence annotation: lacIq
sequence annotation: -10
sequence annotation: -35
sequence annotation: K180009
sequence annotation: C0179
sequence annotation: K180000
sequence: https://synbiohub.org/public/igem/BBa_K180017_sequence/1

K180019
type: SequenceAnnotation
persistent identity: annotation2058464
display id: annotation2058464
version: 1
top level: BBa_K180017
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K180017/annotation2058464/range2058464/1

https://synbiohub.org/public/igem/BBa_K180017/annotation2058464/range2058464/1
type: Range
persistent identity: range2058464
display id: range2058464
version: 1
direction: direction/forward
top level: BBa_K180017
start: 930
end: 3858
orientation: inline

mBanana
type: SequenceAnnotation
persistent identity: annotation2058470
display id: annotation2058470
version: 1
top level: BBa_K180017
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K180017/annotation2058470/range2058470/1

https://synbiohub.org/public/igem/BBa_K180017/annotation2058470/range2058470/1
type: Range
persistent identity: range2058470
display id: range2058470
version: 1
direction: direction/forward
top level: BBa_K180017
start: 2403
end: 3110
orientation: inline

conserved
type: SequenceAnnotation
persistent identity: annotation2058467
display id: annotation2058467
version: 1
top level: BBa_K180017
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K180017/annotation2058467/range2058467/1

https://synbiohub.org/public/igem/BBa_K180017/annotation2058467/range2058467/1
type: Range
persistent identity: range2058467
display id: range2058467
version: 1
direction: direction/forward
top level: BBa_K180017
start: 2389
end: 2392
orientation: inline

conserved
type: SequenceAnnotation
persistent identity: annotation2058458
display id: annotation2058458
version: 1
top level: BBa_K180017
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K180017/annotation2058458/range2058458/1

https://synbiohub.org/public/igem/BBa_K180017/annotation2058458/range2058458/1
type: Range
persistent identity: range2058458
display id: range2058458
version: 1
direction: direction/reverse
top level: BBa_K180017
start: 871
end: 874
orientation: inline

C0161
type: SequenceAnnotation
persistent identity: annotation2058472
display id: annotation2058472
version: 1
top level: BBa_K180017
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K180017/annotation2058472/range2058472/1

https://synbiohub.org/public/igem/BBa_K180017/annotation2058472/range2058472/1
type: Range
persistent identity: range2058472
display id: range2058472
version: 1
direction: direction/forward
top level: BBa_K180017
start: 3137
end: 3721
orientation: inline

J23119
type: SequenceAnnotation
persistent identity: annotation2058459
display id: annotation2058459
version: 1
top level: BBa_K180017
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K180017/annotation2058459/range2058459/1

https://synbiohub.org/public/igem/BBa_K180017/annotation2058459/range2058459/1
type: Range
persistent identity: range2058459
display id: range2058459
version: 1
direction: direction/reverse
top level: BBa_K180017
start: 887
end: 921
orientation: inline

K180018
type: SequenceAnnotation
persistent identity: annotation2058454
display id: annotation2058454
version: 1
top level: BBa_K180017
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K180017/annotation2058454/range2058454/1

https://synbiohub.org/public/igem/BBa_K180017/annotation2058454/range2058454/1
type: Range
persistent identity: range2058454
display id: range2058454
version: 1
direction: direction/reverse
top level: BBa_K180017
start: 1
end: 921
orientation: inline

Ptac
type: SequenceAnnotation
persistent identity: annotation2058465
display id: annotation2058465
version: 1
top level: BBa_K180017
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K180017/annotation2058465/range2058465/1

https://synbiohub.org/public/igem/BBa_K180017/annotation2058465/range2058465/1
type: Range
persistent identity: range2058465
display id: range2058465
version: 1
direction: direction/forward
top level: BBa_K180017
start: 2254
end: 2281
orientation: inline

lacI
type: SequenceAnnotation
persistent identity: annotation2058473
display id: annotation2058473
version: 1
top level: BBa_K180017
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K180017/annotation2058473/range2058473/1

https://synbiohub.org/public/igem/BBa_K180017/annotation2058473/range2058473/1
type: Range
persistent identity: range2058473
display id: range2058473
version: 1
direction: direction/forward
top level: BBa_K180017
start: 3137
end: 3721
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation2058457
display id: annotation2058457
version: 1
top level: BBa_K180017
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K180017/annotation2058457/range2058457/1

https://synbiohub.org/public/igem/BBa_K180017/annotation2058457/range2058457/1
type: Range
persistent identity: range2058457
display id: range2058457
version: 1
direction: direction/reverse
top level: BBa_K180017
start: 867
end: 878
orientation: inline

lasR
type: SequenceAnnotation
persistent identity: annotation2058455
display id: annotation2058455
version: 1
top level: BBa_K180017
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K180017/annotation2058455/range2058455/1

https://synbiohub.org/public/igem/BBa_K180017/annotation2058455/range2058455/1
type: Range
persistent identity: range2058455
display id: range2058455
version: 1
direction: direction/reverse
top level: BBa_K180017
start: 138
end: 860
orientation: inline

B0015
type: SequenceAnnotation
persistent identity: annotation2058453
display id: annotation2058453
version: 1
top level: BBa_K180017
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K180017/annotation2058453/range2058453/1

https://synbiohub.org/public/igem/BBa_K180017/annotation2058453/range2058453/1
type: Range
persistent identity: range2058453
display id: range2058453
version: 1
direction: direction/reverse
top level: BBa_K180017
start: 1
end: 129
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation2058466
display id: annotation2058466
version: 1
top level: BBa_K180017
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K180017/annotation2058466/range2058466/1

https://synbiohub.org/public/igem/BBa_K180017/annotation2058466/range2058466/1
type: Range
persistent identity: range2058466
display id: range2058466
version: 1
direction: direction/forward
top level: BBa_K180017
start: 2385
end: 2396
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation2058471
display id: annotation2058471
version: 1
top level: BBa_K180017
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K180017/annotation2058471/range2058471/1

https://synbiohub.org/public/igem/BBa_K180017/annotation2058471/range2058471/1
type: Range
persistent identity: range2058471
display id: range2058471
version: 1
direction: direction/forward
top level: BBa_K180017
start: 3119
end: 3130
orientation: inline

lacIq
type: SequenceAnnotation
persistent identity: annotation2058462
display id: annotation2058462
version: 1
top level: BBa_K180017
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K180017/annotation2058462/range2058462/1

https://synbiohub.org/public/igem/BBa_K180017/annotation2058462/range2058462/1
type: Range
persistent identity: range2058462
display id: range2058462
version: 1
direction: direction/forward
top level: BBa_K180017
start: 930
end: 2011
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2058460
display id: annotation2058460
version: 1
top level: BBa_K180017
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K180017/annotation2058460/range2058460/1

https://synbiohub.org/public/igem/BBa_K180017/annotation2058460/range2058460/1
type: Range
persistent identity: range2058460
display id: range2058460
version: 1
direction: direction/reverse
top level: BBa_K180017
start: 893
end: 898
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2058461
display id: annotation2058461
version: 1
top level: BBa_K180017
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K180017/annotation2058461/range2058461/1

https://synbiohub.org/public/igem/BBa_K180017/annotation2058461/range2058461/1
type: Range
persistent identity: range2058461
display id: range2058461
version: 1
direction: direction/reverse
top level: BBa_K180017
start: 916
end: 921
orientation: inline

K180009
type: SequenceAnnotation
persistent identity: annotation2058469
display id: annotation2058469
version: 1
top level: BBa_K180017
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K180017/annotation2058469/range2058469/1

https://synbiohub.org/public/igem/BBa_K180017/annotation2058469/range2058469/1
type: Range
persistent identity: range2058469
display id: range2058469
version: 1
direction: direction/forward
top level: BBa_K180017
start: 2403
end: 3110
orientation: inline

C0179
type: SequenceAnnotation
persistent identity: annotation2058456
display id: annotation2058456
version: 1
top level: BBa_K180017
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K180017/annotation2058456/range2058456/1

https://synbiohub.org/public/igem/BBa_K180017/annotation2058456/range2058456/1
type: Range
persistent identity: range2058456
display id: range2058456
version: 1
direction: direction/reverse
top level: BBa_K180017
start: 138
end: 860
orientation: inline

B0015
type: SequenceAnnotation
persistent identity: annotation2058474
display id: annotation2058474
version: 1
top level: BBa_K180017
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K180017/annotation2058474/range2058474/1

https://synbiohub.org/public/igem/BBa_K180017/annotation2058474/range2058474/1
type: Range
persistent identity: range2058474
display id: range2058474
version: 1
direction: direction/forward
top level: BBa_K180017
start: 3730
end: 3858
orientation: inline

K180000
type: SequenceAnnotation
persistent identity: annotation2058463
display id: annotation2058463
version: 1
top level: BBa_K180017
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K180017/annotation2058463/range2058463/1

https://synbiohub.org/public/igem/BBa_K180017/annotation2058463/range2058463/1
type: Range
persistent identity: range2058463
display id: range2058463
version: 1
direction: direction/forward
top level: BBa_K180017
start: 930
end: 2376
orientation: inline

conserved
type: SequenceAnnotation
persistent identity: annotation2058468
display id: annotation2058468
version: 1
top level: BBa_K180017
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K180017/annotation2058468/range2058468/1

https://synbiohub.org/public/igem/BBa_K180017/annotation2058468/range2058468/1
type: Range
persistent identity: range2058468
display id: range2058468
version: 1
direction: direction/forward
top level: BBa_K180017
start: 2390
end: 2393
orientation: inline

BBa_K823008
type: ComponentDefinition
persistent identity: BBa K823008
display id: BBa_K823008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Anderson promoter J23106
creator: Korinna Kraft
created: 2012-09-07T11:00:00Z
modified: 2015-07-07T11:47:05Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1081_
m user id: 4206
owner id: 12081
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Anderson promoter J23106 without RFP in backbone vector pSB1C3 to easily fuse the promoter with other reporters e.g. the lux operon.
mutable notes: no considerations
mutable provenance: Partsregistry
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: J23106
sequence annotation: -10 box
sequence annotation: -35 box
sequence: https://synbiohub.org/public/igem/BBa_K823008_sequence/1

J23106
type: SequenceAnnotation
persistent identity: annotation2182530
display id: annotation2182530
version: 1
top level: BBa_K823008
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K823008/annotation2182530/range2182530/1

https://synbiohub.org/public/igem/BBa_K823008/annotation2182530/range2182530/1
type: Range
persistent identity: range2182530
display id: range2182530
version: 1
direction: direction/forward
top level: BBa_K823008
start: 1
end: 35
orientation: inline

-10 box
type: SequenceAnnotation
persistent identity: annotation2182529
display id: annotation2182529
version: 1
top level: BBa_K823008
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K823008/annotation2182529/range2182529/1

https://synbiohub.org/public/igem/BBa_K823008/annotation2182529/range2182529/1
type: Range
persistent identity: range2182529
display id: range2182529
version: 1
direction: direction/forward
top level: BBa_K823008
start: 24
end: 29
orientation: inline

-35 box
type: SequenceAnnotation
persistent identity: annotation2182528
display id: annotation2182528
version: 1
top level: BBa_K823008
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K823008/annotation2182528/range2182528/1

https://synbiohub.org/public/igem/BBa_K823008/annotation2182528/range2182528/1
type: Range
persistent identity: range2182528
display id: range2182528
version: 1
direction: direction/forward
top level: BBa_K823008
start: 1
end: 6
orientation: inline

lux pL
type: ComponentDefinition
persistent identity: BBa R0063
display id: BBa_R0063
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter (luxR & HSL regulated -- lux pL)
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:15Z
discontinued: false
dominant: true
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: The lux cassette of V. fischeri contains a left and a right promoter. The left promoter gives weak constitutive expression of downstream genes.This expression is down-regulated by the action of the Lux repressor, LuxR. Two molecules of LuxR protein form a complex with two molecules the signalling compound homoserine lactone (HSL). This complex binds to a palindromic site on the promoter, increasing the rate of transcription from Pr, repressing transcription from Pl
mutable notes: This promoter is based on the Vibrio fischeri quorum sensing gene promoters. Two genes LuxI and LuxR and transcribed in opposite directions as shown below. The original sequence from which the parts <bb_part>BBa_R0062</bb_part> and <bb_part>BBa_R0063</bb_part> were derived is shown in the picture below.Includes most of Lux reulatory region, including the LuxR binding site which activates the right promoter. A putative LuxR autorepression binding site is also identified adjacent to the -10 site of the right promoter. This 2nd site has 55% identity with the first site. Putative inverted repeats (of size 18-27 bp) also exist between these two sites (not marked above), which may represent binding sites for other regulatory proteins. <img src="<bb_file>Image01.gif</bb_file>" width="614" height="362"> Unspecified.
mutable provenance: V. fischeri.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: start
sequence annotation: BBa_R0063
sequence annotation: Putative LuxR/HSL
sequence annotation: LuxR/HSL
sequence annotation: -10
sequence annotation: -35
sequence: https://synbiohub.org/public/igem/BBa_R0063_sequence/1

start
type: SequenceAnnotation
persistent identity: annotation2054
display id: annotation2054
version: 1
top level: lux pL
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_R0063/annotation2054/range2054/1

https://synbiohub.org/public/igem/BBa_R0063/annotation2054/range2054/1
type: Range
persistent identity: range2054
display id: range2054
version: 1
direction: direction/forward
top level: lux pL
start: 128
end: 128
orientation: inline

BBa_R0063
type: SequenceAnnotation
persistent identity: annotation7071
display id: annotation7071
version: 1
top level: lux pL
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_R0063/annotation7071/range7071/1

https://synbiohub.org/public/igem/BBa_R0063/annotation7071/range7071/1
type: Range
persistent identity: range7071
display id: range7071
version: 1
direction: direction/forward
top level: lux pL
start: 1
end: 151
orientation: inline

Putative LuxR/HSL
type: SequenceAnnotation
persistent identity: annotation2055
display id: annotation2055
version: 1
top level: lux pL
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0063/annotation2055/range2055/1

https://synbiohub.org/public/igem/BBa_R0063/annotation2055/range2055/1
type: Range
persistent identity: range2055
display id: range2055
version: 1
direction: direction/forward
top level: lux pL
start: 130
end: 149
orientation: inline

LuxR/HSL
type: SequenceAnnotation
persistent identity: annotation2051
display id: annotation2051
version: 1
top level: lux pL
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0063/annotation2051/range2051/1

https://synbiohub.org/public/igem/BBa_R0063/annotation2051/range2051/1
type: Range
persistent identity: range2051
display id: range2051
version: 1
direction: direction/forward
top level: lux pL
start: 1
end: 20
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2052
display id: annotation2052
version: 1
top level: lux pL
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0063/annotation2052/range2052/1

https://synbiohub.org/public/igem/BBa_R0063/annotation2052/range2052/1
type: Range
persistent identity: range2052
display id: range2052
version: 1
direction: direction/forward
top level: lux pL
start: 115
end: 122
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2053
display id: annotation2053
version: 1
top level: lux pL
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0063/annotation2053/range2053/1

https://synbiohub.org/public/igem/BBa_R0063/annotation2053/range2053/1
type: Range
persistent identity: range2053
display id: range2053
version: 1
direction: direction/forward
top level: lux pL
start: 89
end: 94
orientation: inline

Zif23
type: ComponentDefinition
persistent identity: BBa R2000
display id: BBa_R2000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter, Zif23 regulated, test: between
creator: Ronny Krashinsky
created: 2004-01-29T12:00:00Z
modified: 2015-05-08T01:14:15Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Promoter with standard -10 and -35 sites. A symmetric Zif23 dimer target site is between the -10 and -35 sites.
mutable notes: consensus sequences based on [Jensen98].
mutable provenance: synthetic
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: (+1)
sequence annotation: consensus
sequence annotation: -10
sequence annotation: Zif23
sequence annotation: -35
sequence: https://synbiohub.org/public/igem/BBa_R2000_sequence/1

(+1)
type: SequenceAnnotation
persistent identity: annotation318102
display id: annotation318102
version: 1
top level: Zif23
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_R2000/annotation318102/range318102/1

https://synbiohub.org/public/igem/BBa_R2000/annotation318102/range318102/1
type: Range
persistent identity: range318102
display id: range318102
version: 1
direction: direction/forward
top level: Zif23
start: 45
end: 45
orientation: inline

consensus
type: SequenceAnnotation
persistent identity: annotation318100
display id: annotation318100
version: 1
top level: Zif23
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_R2000/annotation318100/range318100/1

https://synbiohub.org/public/igem/BBa_R2000/annotation318100/range318100/1
type: Range
persistent identity: range318100
display id: range318100
version: 1
direction: direction/forward
top level: Zif23
start: 1
end: 9
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation318098
display id: annotation318098
version: 1
top level: Zif23
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R2000/annotation318098/range318098/1

https://synbiohub.org/public/igem/BBa_R2000/annotation318098/range318098/1
type: Range
persistent identity: range318098
display id: range318098
version: 1
direction: direction/forward
top level: Zif23
start: 33
end: 38
orientation: inline

Zif23
type: SequenceAnnotation
persistent identity: annotation318099
display id: annotation318099
version: 1
top level: Zif23
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R2000/annotation318099/range318099/1

https://synbiohub.org/public/igem/BBa_R2000/annotation318099/range318099/1
type: Range
persistent identity: range318099
display id: range318099
version: 1
direction: direction/forward
top level: Zif23
start: 20
end: 33
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation318094
display id: annotation318094
version: 1
top level: Zif23
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R2000/annotation318094/range318094/1

https://synbiohub.org/public/igem/BBa_R2000/annotation318094/range318094/1
type: Range
persistent identity: range318094
display id: range318094
version: 1
direction: direction/forward
top level: Zif23
start: 10
end: 15
orientation: inline

BBa_K180007
type: ComponentDefinition
persistent identity: BBa K180007
display id: BBa_K180007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Game of Life - Secondary plasmid [tac pR, LuxI generator]
creator: Richard Wilson, Stephanie Chambers
created: 2009-10-15T11:00:00Z
modified: 2015-05-08T01:11:06Z
discontinued: false
dominant: false
experience: experience/None
group u list: _278_
m user id: 0
owner id: 4082
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: iGEM2009 Team:Southampton Game of Life secondary plasmid.
mutable notes: Regulated expression of LuxI (C0161) by TAC pR (K180000), a functional hybrid lac/trp promoter.
mutable provenance: See relevant basic biobrick parts (K180000, B0034, C0161, B0015).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_C0161
component: BBa_K180000
component: BBa_B0010
component: BBa_B0034
component: BBa_B0012
sequence annotation: BBa_K180000
sequence annotation: BBa_C0161
sequence annotation: BBa_B0034
sequence annotation: BBa_B0012
sequence annotation: BBa_B0010
sequence: https://synbiohub.org/public/igem/BBa_K180007_sequence/1

BBa_C0161
type: Component
persistent identity: component2046432
display id: component2046432
version: 1
top level: BBa_K180007
access: public
definition: https://synbiohub.org/public/igem/BBa_C0161/1

BBa_K180000
type: Component
persistent identity: component2046428
display id: component2046428
version: 1
top level: BBa_K180007
access: public
definition: BBa_K180000

BBa_B0010
type: Component
persistent identity: component2046433
display id: component2046433
version: 1
top level: BBa_K180007
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0034
type: Component
persistent identity: component2046430
display id: component2046430
version: 1
top level: BBa_K180007
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0012
type: Component
persistent identity: component2046435
display id: component2046435
version: 1
top level: BBa_K180007
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_K180000
type: SequenceAnnotation
persistent identity: annotation2046428
display id: annotation2046428
version: 1
top level: BBa_K180007
location: https://synbiohub.org/public/igem/BBa_K180007/annotation2046428/range2046428/1
component: BBa_K180000

https://synbiohub.org/public/igem/BBa_K180007/annotation2046428/range2046428/1
type: Range
persistent identity: range2046428
display id: range2046428
version: 1
direction: direction/reverse
top level: BBa_K180007
start: 1
end: 1447
orientation: inline

BBa_C0161
type: SequenceAnnotation
persistent identity: annotation2046432
display id: annotation2046432
version: 1
top level: BBa_K180007
location: https://synbiohub.org/public/igem/BBa_K180007/annotation2046432/range2046432/1
component: BBa_C0161

https://synbiohub.org/public/igem/BBa_K180007/annotation2046432/range2046432/1
type: Range
persistent identity: range2046432
display id: range2046432
version: 1
direction: direction/reverse
top level: BBa_K180007
start: 1474
end: 2058
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2046430
display id: annotation2046430
version: 1
top level: BBa_K180007
location: https://synbiohub.org/public/igem/BBa_K180007/annotation2046430/range2046430/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K180007/annotation2046430/range2046430/1
type: Range
persistent identity: range2046430
display id: range2046430
version: 1
direction: direction/reverse
top level: BBa_K180007
start: 1456
end: 1467
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2046435
display id: annotation2046435
version: 1
top level: BBa_K180007
location: https://synbiohub.org/public/igem/BBa_K180007/annotation2046435/range2046435/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K180007/annotation2046435/range2046435/1
type: Range
persistent identity: range2046435
display id: range2046435
version: 1
direction: direction/forward
top level: BBa_K180007
start: 2155
end: 2195
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2046433
display id: annotation2046433
version: 1
top level: BBa_K180007
location: https://synbiohub.org/public/igem/BBa_K180007/annotation2046433/range2046433/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K180007/annotation2046433/range2046433/1
type: Range
persistent identity: range2046433
display id: range2046433
version: 1
direction: direction/forward
top level: BBa_K180007
start: 2067
end: 2146
orientation: inline

BBa_K112318
type: ComponentDefinition
persistent identity: BBa K112318
display id: BBa_K112318
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: {< bolA promoter>} in BBb format
creator: Aron Lau
created: 2008-10-20T11:00:00Z
modified: 2015-05-08T01:09:16Z
discontinued: false
dominant: false
group u list: _224_
m user id: 0
owner id: 2999
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: bolA promoter is a promoter where levels of expression are inversely proportional to the growth rate. To read more, go to following link [http://www.pubmedcentral.nih.gov/picrender.fcgi?artid=402084&blobtype=pdf] This part is actually in BBb format. ---- This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at: [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
mutable notes: PCR al0017/al0018 on MG1655 (467bp, EcoRI/BamHI/DpnI) Sub into pBca1256 (EcoRI/BamHI, 2472+910, L) Product is pBca1256-K112318 ----------------------------------------------- al0017 Forward Biobricking of bolA promoter CCATAGAATTCatgAGATCTtgctgtggcagtgtaatcgtc al0018 Reverse Biobricking of bolA promoter CGttaGGATCCcttctatccgctcacgtatc
mutable provenance: MG1655
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K112318_sequence/1

BBa_K112322
type: ComponentDefinition
persistent identity: BBa K112322
display id: BBa_K112322
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: {Pdps} in BBb format
creator: Aron Lau
created: 2008-10-21T11:00:00Z
modified: 2015-05-08T01:09:16Z
discontinued: false
dominant: false
group u list: _224_
m user id: 0
owner id: 2999
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Pdps promoter. This part is actually in BBb format. ---- This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at: [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
mutable notes: PCR JH089F/JH089R on Salmonella L2. (379 bp, EcoRI/BamHI/DpnI) Sub into pBjh1601CK (EcoRI/BamHI, 3134+910, L) Product is pBjh1601CK-K112322 ----------------------------------------------- JH089F Forward biobricking of {Pdps} cgataGAATTCatgAGATCTaacgattcgacgcgaaccgc JH089R Reverse biobricking of {Pdps} cgttaGGATCCaatctcatatcctcttgatg
mutable provenance: Salmonella L2
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K112322_sequence/1

Kat
type: ComponentDefinition
persistent identity: BBa I14034
display id: BBa_I14034
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: P(Kat)
creator: Vikram Vijayan, Allen Hsu, Lawrence Fomundam
created: 2004-08-03T11:00:00Z
modified: 2015-08-31T04:07:38Z
discontinued: false
dominant: false
experience: experience/None
group u list: _4_
m user id: 0
owner id: 171
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Constitutive Promoter, Medium Transcription
mutable provenance: nnpp promoter 3 from pSB2K3
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: P(Kat)
sequence: https://synbiohub.org/public/igem/BBa_I14034_sequence/1

P(Kat)
type: SequenceAnnotation
persistent identity: annotation1023116
display id: annotation1023116
version: 1
top level: Kat
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I14034/annotation1023116/range1023116/1

https://synbiohub.org/public/igem/BBa_I14034/annotation1023116/range1023116/1
type: Range
persistent identity: range1023116
display id: range1023116
version: 1
direction: direction/forward
top level: Kat
start: 1
end: 45
orientation: inline

pLac(B4)
type: ComponentDefinition
persistent identity: BBa K137125
display id: BBa_K137125
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LacI-repressed promoter B4
creator: Josh Michener
created: 2008-10-07T11:00:00Z
modified: 2015-05-08T01:10:11Z
discontinued: false
dominant: false
experience: experience/None
group u list: _187_
m user id: 0
owner id: 253
owning group id: 6
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: LacI-repressible promoter B4 developed by Sidney Cox (http://www.nature.com/msb/journal/v3/n1/full/msb4100187.html). Showed a regulatory range (ON/OFF) of 2.5x10^4.
mutable provenance: Annealed from oligos.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K137125_sequence/1

BBa_I739107
type: ComponentDefinition
persistent identity: BBa I739107
display id: BBa_I739107
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Double Promoter (cI, negative / LacI, negative)
creator: Stefan Luzi
created: 2007-10-16T11:00:00Z
modified: 2015-08-31T04:08:01Z
discontinued: false
dominant: false
group u list: _124_
m user id: 0
owner id: 2183
owning group id: 62
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: asdf
mutable notes: sadf
mutable provenance: Synthetic DNA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: R0010
sequence annotation: LacI binding site
sequence annotation: R0051
sequence: https://synbiohub.org/public/igem/BBa_I739107_sequence/1

R0010
type: SequenceAnnotation
persistent identity: annotation1948512
display id: annotation1948512
version: 1
top level: BBa_I739107
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_I739107/annotation1948512/range1948512/1

https://synbiohub.org/public/igem/BBa_I739107/annotation1948512/range1948512/1
type: Range
persistent identity: range1948512
display id: range1948512
version: 1
direction: direction/forward
top level: BBa_I739107
start: 44
end: 78
orientation: inline

LacI binding site
type: SequenceAnnotation
persistent identity: annotation1948511
display id: annotation1948511
version: 1
top level: BBa_I739107
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I739107/annotation1948511/range1948511/1

https://synbiohub.org/public/igem/BBa_I739107/annotation1948511/range1948511/1
type: Range
persistent identity: range1948511
display id: range1948511
version: 1
direction: direction/forward
top level: BBa_I739107
start: 44
end: 78
orientation: inline

R0051
type: SequenceAnnotation
persistent identity: annotation1948510
display id: annotation1948510
version: 1
top level: BBa_I739107
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I739107/annotation1948510/range1948510/1

https://synbiohub.org/public/igem/BBa_I739107/annotation1948510/range1948510/1
type: Range
persistent identity: range1948510
display id: range1948510
version: 1
direction: direction/forward
top level: BBa_I739107
start: 1
end: 43
orientation: inline

BBa_K1682004
type: ComponentDefinition
persistent identity: BBa K1682004
display id: BBa_K1682004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PkdpF[-15, T>G]
creator: iGEM15_HKUST-Rice
created: 2015-08-09T11:00:00Z
modified: 2015-09-18T09:16:31Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2100_
m user id: 8758
owner id: 21235
owning group id: 9
status: status/Planning
bookmark: false
mutable description: kdpFp[-15, T>G]
mutable notes: kdpFp[-15, T>G]
mutable provenance: kdpFp[-15, T>G]
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: phospho-KdpE binding site
sequence annotation: -10
sequence annotation: -35
sequence annotation: EcoRI Illegal site removed T->G
sequence: https://synbiohub.org/public/igem/BBa_K1682004_sequence/1

phospho-KdpE binding site
type: SequenceAnnotation
persistent identity: annotation2473581
display id: annotation2473581
version: 1
top level: BBa_K1682004
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1682004/annotation2473581/range2473581/1

https://synbiohub.org/public/igem/BBa_K1682004/annotation2473581/range2473581/1
type: Range
persistent identity: range2473581
display id: range2473581
version: 1
direction: direction/forward
top level: BBa_K1682004
start: 8
end: 24
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2473582
display id: annotation2473582
version: 1
top level: BBa_K1682004
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1682004/annotation2473582/range2473582/1

https://synbiohub.org/public/igem/BBa_K1682004/annotation2473582/range2473582/1
type: Range
persistent identity: range2473582
display id: range2473582
version: 1
direction: direction/forward
top level: BBa_K1682004
start: 69
end: 74
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2473584
display id: annotation2473584
version: 1
top level: BBa_K1682004
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1682004/annotation2473584/range2473584/1

https://synbiohub.org/public/igem/BBa_K1682004/annotation2473584/range2473584/1
type: Range
persistent identity: range2473584
display id: range2473584
version: 1
direction: direction/forward
top level: BBa_K1682004
start: 42
end: 47
orientation: inline

EcoRI Illegal site removed T->G
type: SequenceAnnotation
persistent identity: annotation2475215
display id: annotation2475215
version: 1
top level: BBa_K1682004
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K1682004/annotation2475215/range2475215/1

https://synbiohub.org/public/igem/BBa_K1682004/annotation2475215/range2475215/1
type: Range
persistent identity: range2475215
display id: range2475215
version: 1
direction: direction/forward
top level: BBa_K1682004
start: 65
end: 66
orientation: inline

U097NUL+D0
type: ComponentDefinition
persistent identity: BBa I732410
display id: BBa_I732410
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member (U097NUL+D038O22)
creator: Zhan Jian
created: 2007-09-22T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1572
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: To be later edited if time permits.
mutable notes: To be later edited if time permits.
mutable provenance: To be later edited if time permits.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: consensus elements
sequence annotation: +1
sequence annotation: O2
sequence: https://synbiohub.org/public/igem/BBa_I732410_sequence/1

consensus elements
type: SequenceAnnotation
persistent identity: annotation1944558
display id: annotation1944558
version: 1
top level: U097NUL+D0
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I732410/annotation1944558/range1944558/1

https://synbiohub.org/public/igem/BBa_I732410/annotation1944558/range1944558/1
type: Range
persistent identity: range1944558
display id: range1944558
version: 1
direction: direction/forward
top level: U097NUL+D0
start: 67
end: 137
orientation: inline

+1
type: SequenceAnnotation
persistent identity: annotation1944560
display id: annotation1944560
version: 1
top level: U097NUL+D0
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_I732410/annotation1944560/range1944560/1

https://synbiohub.org/public/igem/BBa_I732410/annotation1944560/range1944560/1
type: Range
persistent identity: range1944560
display id: range1944560
version: 1
direction: direction/forward
top level: U097NUL+D0
start: 137
end: 137
orientation: inline

O2
type: SequenceAnnotation
persistent identity: annotation1944559
display id: annotation1944559
version: 1
top level: U097NUL+D0
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_I732410/annotation1944559/range1944559/1

https://synbiohub.org/public/igem/BBa_I732410/annotation1944559/range1944559/1
type: Range
persistent identity: range1944559
display id: range1944559
version: 1
direction: direction/forward
top level: U097NUL+D0
start: 134
end: 153
orientation: inline

BBa_K091146
type: ComponentDefinition
persistent identity: BBa K091146
display id: BBa_K091146
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pLas/Lux Hybrid Promoter
creator: Erin Feeney
created: 2008-07-16T11:00:00Z
modified: 2015-05-08T01:08:38Z
discontinued: false
dominant: false
experience: experience/None
group u list: _191_
m user id: 0
owner id: 3067
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: K091146 is a modified version of the pLas' promoter (BBa_K091117) containing the addition of a luxR binding site between the -35 and -10 regions. This promoter is expected to be activated in the presence of PAI-1 and LasR and repressed in the presence of AI-1 and LuxR.
mutable notes: The Lux box was placed adjacent to the LasR binding site with the aim of minimizing cross-talk in the presence of both PAI-1 and AI-1; since the LasR site should have a higher affinity, we hope that the binding of PAI-1 will competitively exclude the binding of AI-1.
mutable provenance: The LuxR binding site is from BBa_R0062 and the pLas' promoter is from the region upstream of the LasI gene in Pseudomonas aeruginosa.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: LasR
sequence annotation: -35
sequence annotation: -10
sequence annotation: LuxR
sequence annotation: Transcriptional Start
sequence: https://synbiohub.org/public/igem/BBa_K091146_sequence/1

LasR
type: SequenceAnnotation
persistent identity: annotation1967354
display id: annotation1967354
version: 1
top level: BBa_K091146
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K091146/annotation1967354/range1967354/1

https://synbiohub.org/public/igem/BBa_K091146/annotation1967354/range1967354/1
type: Range
persistent identity: range1967354
display id: range1967354
version: 1
direction: direction/forward
top level: BBa_K091146
start: 75
end: 93
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1967357
display id: annotation1967357
version: 1
top level: BBa_K091146
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K091146/annotation1967357/range1967357/1

https://synbiohub.org/public/igem/BBa_K091146/annotation1967357/range1967357/1
type: Range
persistent identity: range1967357
display id: range1967357
version: 1
direction: direction/forward
top level: BBa_K091146
start: 86
end: 91
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1967356
display id: annotation1967356
version: 1
top level: BBa_K091146
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K091146/annotation1967356/range1967356/1

https://synbiohub.org/public/igem/BBa_K091146/annotation1967356/range1967356/1
type: Range
persistent identity: range1967356
display id: range1967356
version: 1
direction: direction/forward
top level: BBa_K091146
start: 113
end: 118
orientation: inline

LuxR
type: SequenceAnnotation
persistent identity: annotation1967355
display id: annotation1967355
version: 1
top level: BBa_K091146
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K091146/annotation1967355/range1967355/1

https://synbiohub.org/public/igem/BBa_K091146/annotation1967355/range1967355/1
type: Range
persistent identity: range1967355
display id: range1967355
version: 1
direction: direction/forward
top level: BBa_K091146
start: 94
end: 113
orientation: inline

Transcriptional Start
type: SequenceAnnotation
persistent identity: annotation1967358
display id: annotation1967358
version: 1
top level: BBa_K091146
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K091146/annotation1967358/range1967358/1

https://synbiohub.org/public/igem/BBa_K091146/annotation1967358/range1967358/1
type: Range
persistent identity: range1967358
display id: range1967358
version: 1
direction: direction/forward
top level: BBa_K091146
start: 125
end: 125
orientation: inline

BBa_K118011
type: ComponentDefinition
persistent identity: BBa K118011
display id: BBa_K118011
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PcstA (glucose-repressible promoter)
creator: Andrew Hall
created: 2008-08-18T11:00:00Z
modified: 2015-05-08T01:09:36Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _192_
m user id: 0
owner id: 3282
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: This is the promoter for the ''Eschaerichia coli'' JM109 ''cstA'' gene. It includes the CRP-binding site and the RNA polymerase-binding site. Low glucose concentration results in increased activity by adenylate cyclase. cAMP binds to the cAMP receptor protein, which, in its bound form, is able to associate with the promoter and promote transcription of the downstream gene. (''cstA'' encodes the carbon starvation protein.)
mutable notes: No special considerations
mutable provenance: ''Eschaerichia coli'' JM109 genomic DNA
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: cAMP receptor protein binding site
sequence annotation: RNA polymerase binding site
sequence: https://synbiohub.org/public/igem/BBa_K118011_sequence/1

cAMP receptor protein binding site
type: SequenceAnnotation
persistent identity: annotation1972435
display id: annotation1972435
version: 1
top level: BBa_K118011
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K118011/annotation1972435/range1972435/1

https://synbiohub.org/public/igem/BBa_K118011/annotation1972435/range1972435/1
type: Range
persistent identity: range1972435
display id: range1972435
version: 1
direction: direction/forward
top level: BBa_K118011
start: 1
end: 42
orientation: inline

RNA polymerase binding site
type: SequenceAnnotation
persistent identity: annotation1972436
display id: annotation1972436
version: 1
top level: BBa_K118011
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K118011/annotation1972436/range1972436/1

https://synbiohub.org/public/igem/BBa_K118011/annotation1972436/range1972436/1
type: Range
persistent identity: range1972436
display id: range1972436
version: 1
direction: direction/forward
top level: BBa_K118011
start: 101
end: 131
orientation: inline

BBa_K091107
type: ComponentDefinition
persistent identity: BBa K091107
display id: BBa_K091107
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pLux/cI Hybrid Promoter
creator: Xiao Zhu
created: 2008-06-18T11:00:00Z
modified: 2015-05-08T01:08:37Z
discontinued: false
dominant: false
experience: experience/None
group u list: _191_
m user id: 0
owner id: 3130
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This hybrid promoter is designed to respond to activation by LuxR when it is bound to AHL and to repression by the phage lambda cI repressor. The repression needs to win over activation.
mutable notes: This part is based on the idea of replacing the region of the Lux promoter downstream of the -35 with the OR2 cI half-operator, added LuxR-10 region. Based on what we have found up to now, promoters which are constructed just by inserting OR1/2 downstream of Lux are all very leaky.
mutable provenance: this part comes from LuxR promoter and cI lambda promoter.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: cI OR2
sequence annotation: Lux -10
sequence annotation: Lux Box
sequence annotation: Lux -35
sequence: https://synbiohub.org/public/igem/BBa_K091107_sequence/1

cI OR2
type: SequenceAnnotation
persistent identity: annotation1963752
display id: annotation1963752
version: 1
top level: BBa_K091107
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K091107/annotation1963752/range1963752/1

https://synbiohub.org/public/igem/BBa_K091107/annotation1963752/range1963752/1
type: Range
persistent identity: range1963752
display id: range1963752
version: 1
direction: direction/forward
top level: BBa_K091107
start: 26
end: 43
orientation: inline

Lux -10
type: SequenceAnnotation
persistent identity: annotation1963753
display id: annotation1963753
version: 1
top level: BBa_K091107
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K091107/annotation1963753/range1963753/1

https://synbiohub.org/public/igem/BBa_K091107/annotation1963753/range1963753/1
type: Range
persistent identity: range1963753
display id: range1963753
version: 1
direction: direction/forward
top level: BBa_K091107
start: 44
end: 49
orientation: inline

Lux Box
type: SequenceAnnotation
persistent identity: annotation1963750
display id: annotation1963750
version: 1
top level: BBa_K091107
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K091107/annotation1963750/range1963750/1

https://synbiohub.org/public/igem/BBa_K091107/annotation1963750/range1963750/1
type: Range
persistent identity: range1963750
display id: range1963750
version: 1
direction: direction/forward
top level: BBa_K091107
start: 1
end: 20
orientation: inline

Lux -35
type: SequenceAnnotation
persistent identity: annotation1963751
display id: annotation1963751
version: 1
top level: BBa_K091107
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K091107/annotation1963751/range1963751/1

https://synbiohub.org/public/igem/BBa_K091107/annotation1963751/range1963751/1
type: Range
persistent identity: range1963751
display id: range1963751
version: 1
direction: direction/forward
top level: BBa_K091107
start: 20
end: 25
orientation: inline

BBa_J64069
type: ComponentDefinition
persistent identity: BBa J64069
display id: BBa_J64069
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: R0065 with lux box deleted
creator: Jeffrey J Tabor
created: 2008-02-10T12:00:00Z
modified: 2015-05-08T01:08:17Z
discontinued: false
dominant: false
experience: experience/None
group u list: _98_
m user id: 0
owner id: 88
owning group id: 98
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: luxR operator (lux box) was deleted from R0065 and replaced with a BamHI hexamer.
mutable notes: added a bamHI site immediately upstream of -35 in place of lux operator
mutable provenance: constructed
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -10
sequence annotation: +1
sequence annotation: -35
sequence: https://synbiohub.org/public/igem/BBa_J64069_sequence/1

-10
type: SequenceAnnotation
persistent identity: annotation1961161
display id: annotation1961161
version: 1
top level: BBa_J64069
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J64069/annotation1961161/range1961161/1

https://synbiohub.org/public/igem/BBa_J64069/annotation1961161/range1961161/1
type: Range
persistent identity: range1961161
display id: range1961161
version: 1
direction: direction/forward
top level: BBa_J64069
start: 34
end: 39
orientation: inline

+1
type: SequenceAnnotation
persistent identity: annotation1961162
display id: annotation1961162
version: 1
top level: BBa_J64069
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J64069/annotation1961162/range1961162/1

https://synbiohub.org/public/igem/BBa_J64069/annotation1961162/range1961162/1
type: Range
persistent identity: range1961162
display id: range1961162
version: 1
direction: direction/forward
top level: BBa_J64069
start: 46
end: 46
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1961160
display id: annotation1961160
version: 1
top level: BBa_J64069
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J64069/annotation1961160/range1961160/1

https://synbiohub.org/public/igem/BBa_J64069/annotation1961160/range1961160/1
type: Range
persistent identity: range1961160
display id: range1961160
version: 1
direction: direction/forward
top level: BBa_J64069
start: 12
end: 17
orientation: inline

const.
type: ComponentDefinition
persistent identity: BBa R1074
display id: BBa_R1074
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Constitutive Promoter I
creator: Chris Walsh (Fighting Darwins)
created: 2004-01-27T12:00:00Z
modified: 2015-05-08T01:14:15Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: constitutive promoter which outputs .3 TIPs point mutants of wild type rrnB promoter P1
mutable provenance: Gaal, Gourse, et al.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -10
sequence annotation: -35
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_R1074_sequence/1

-10
type: SequenceAnnotation
persistent identity: annotation309011
display id: annotation309011
version: 1
top level: const.
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R1074/annotation309011/range309011/1

https://synbiohub.org/public/igem/BBa_R1074/annotation309011/range309011/1
type: Range
persistent identity: range309011
display id: range309011
version: 1
direction: direction/forward
top level: const.
start: 35
end: 40
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation309012
display id: annotation309012
version: 1
top level: const.
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R1074/annotation309012/range309012/1

https://synbiohub.org/public/igem/BBa_R1074/annotation309012/range309012/1
type: Range
persistent identity: range309012
display id: range309012
version: 1
direction: direction/forward
top level: const.
start: 15
end: 20
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2214002
display id: annotation2214002
version: 1
top level: const.
role: SO:0000807
role: feature/barcode
location: https://synbiohub.org/public/igem/BBa_R1074/annotation2214002/range2214002/1

https://synbiohub.org/public/igem/BBa_R1074/annotation2214002/range2214002/1
type: Range
persistent identity: range2214002
display id: range2214002
version: 1
direction: direction/forward
top level: const.
start: 50
end: 74
orientation: inline

U037O11+D0
type: ComponentDefinition
persistent identity: BBa I732447
display id: BBa_I732447
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member (U037O11+D050O22)
creator: Zhan Jian
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732447_sequence/1

MioC
type: ComponentDefinition
persistent identity: BBa K101017
display id: BBa_K101017
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: MioC Promoter (DNAa-Repressed Promoter)
creator: Karl Petersen
created: 2008-10-27T12:00:00Z
modified: 2015-05-08T01:08:42Z
discontinued: false
dominant: false
experience: experience/None
group u list: _205_
m user id: 0
owner id: 2728
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: The MioC promoter is a cell-cycle dependent promoter that is repressed before initiation of replication and depressed shortly after. The regulation of the MioC gene is overseen by several DNAa proteins, which bind along the promoter. This particular MioC promoter was isolated from K-12 (JM109). This promoter can be used in series with a reporter to signal only during a given part of the cell cycle.
mutable notes: Site directed mutagenesis was used to create a Spe1 restriction site.
mutable provenance: The source of this part is from the genomic dna of E.coli (JM109).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: DNAa Binding Box
sequence annotation: MioC Promoter
sequence: https://synbiohub.org/public/igem/BBa_K101017_sequence/1

DNAa Binding Box
type: SequenceAnnotation
persistent identity: annotation1992605
display id: annotation1992605
version: 1
top level: MioC
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K101017/annotation1992605/range1992605/1

https://synbiohub.org/public/igem/BBa_K101017/annotation1992605/range1992605/1
type: Range
persistent identity: range1992605
display id: range1992605
version: 1
direction: direction/forward
top level: MioC
start: 283
end: 292
orientation: inline

DNAa Binding Box
type: SequenceAnnotation
persistent identity: annotation1992609
display id: annotation1992609
version: 1
top level: MioC
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K101017/annotation1992609/range1992609/1

https://synbiohub.org/public/igem/BBa_K101017/annotation1992609/range1992609/1
type: Range
persistent identity: range1992609
display id: range1992609
version: 1
direction: direction/forward
top level: MioC
start: 159
end: 167
orientation: inline

MioC Promoter
type: SequenceAnnotation
persistent identity: annotation1992615
display id: annotation1992615
version: 1
top level: MioC
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K101017/annotation1992615/range1992615/1

https://synbiohub.org/public/igem/BBa_K101017/annotation1992615/range1992615/1
type: Range
persistent identity: range1992615
display id: range1992615
version: 1
direction: direction/forward
top level: MioC
start: 131
end: 319
orientation: inline

DNAa Binding Box
type: SequenceAnnotation
persistent identity: annotation1992607
display id: annotation1992607
version: 1
top level: MioC
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K101017/annotation1992607/range1992607/1

https://synbiohub.org/public/igem/BBa_K101017/annotation1992607/range1992607/1
type: Range
persistent identity: range1992607
display id: range1992607
version: 1
direction: direction/forward
top level: MioC
start: 273
end: 279
orientation: inline

DNAa Binding Box
type: SequenceAnnotation
persistent identity: annotation1992601
display id: annotation1992601
version: 1
top level: MioC
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K101017/annotation1992601/range1992601/1

https://synbiohub.org/public/igem/BBa_K101017/annotation1992601/range1992601/1
type: Range
persistent identity: range1992601
display id: range1992601
version: 1
direction: direction/forward
top level: MioC
start: 295
end: 303
orientation: inline

BBa_J23117
type: ComponentDefinition
persistent identity: BBa J23117
display id: BBa_J23117
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: constitutive promoter family member
creator: John Anderson
created: 2006-08-16T11:00:00Z
modified: 2015-08-31T04:08:40Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: Later
mutable notes: N/A
mutable provenance: Later
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J23117_sequence/1

BBa_K1682012
type: ComponentDefinition
persistent identity: BBa K1682012
display id: BBa_K1682012
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PphoA promoter
creator: iGEM15_HKUST-Rice
created: 2015-09-01T11:00:00Z
modified: 2015-09-18T10:16:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2100_
m user id: 21235
owner id: 26069
owning group id: 9
status: status/Planning
bookmark: false
mutable description: PphoA promoter is a phosphate-responsive promoter. In high concentration of phosphate, it will be repressed while in low phosphate concentration, it will be activated.
mutable notes: N/A
mutable provenance: Native in E. coli
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -10
sequence annotation: -35
sequence annotation: phospho-phoB
sequence: https://synbiohub.org/public/igem/BBa_K1682012_sequence/1

-10
type: SequenceAnnotation
persistent identity: annotation2476004
display id: annotation2476004
version: 1
top level: BBa_K1682012
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1682012/annotation2476004/range2476004/1

https://synbiohub.org/public/igem/BBa_K1682012/annotation2476004/range2476004/1
type: Range
persistent identity: range2476004
display id: range2476004
version: 1
direction: direction/forward
top level: BBa_K1682012
start: 50
end: 56
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2476003
display id: annotation2476003
version: 1
top level: BBa_K1682012
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1682012/annotation2476003/range2476003/1

https://synbiohub.org/public/igem/BBa_K1682012/annotation2476003/range2476003/1
type: Range
persistent identity: range2476003
display id: range2476003
version: 1
direction: direction/forward
top level: BBa_K1682012
start: 28
end: 33
orientation: inline

phospho-phoB
type: SequenceAnnotation
persistent identity: annotation2476002
display id: annotation2476002
version: 1
top level: BBa_K1682012
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1682012/annotation2476002/range2476002/1

https://synbiohub.org/public/igem/BBa_K1682012/annotation2476002/range2476002/1
type: Range
persistent identity: range2476002
display id: range2476002
version: 1
direction: direction/forward
top level: BBa_K1682012
start: 21
end: 40
orientation: inline

BBa_K119002
type: ComponentDefinition
persistent identity: BBa K119002
display id: BBa_K119002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RcnR operator (represses RcnA)
creator: Libertad Pantoja
created: 2008-09-27T11:00:00Z
modified: 2015-05-08T01:09:38Z
discontinued: false
dominant: false
group u list: _235_
m user id: 0
owner id: 3054
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Operator for RcnR(YohL) the negative regulator of niquel extrusion pump(RcnA) in E.coli
mutable notes: We needed the RcnR operator without its promoter, so it was necessary to predict the promoter and discard it from the sequence.
mutable provenance: E.coli genome
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: RcnRop
sequence: https://synbiohub.org/public/igem/BBa_K119002_sequence/1

RcnRop
type: SequenceAnnotation
persistent identity: annotation1977507
display id: annotation1977507
version: 1
top level: BBa_K119002
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K119002/annotation1977507/range1977507/1

https://synbiohub.org/public/igem/BBa_K119002/annotation1977507/range1977507/1
type: Range
persistent identity: range1977507
display id: range1977507
version: 1
direction: direction/forward
top level: BBa_K119002
start: 1
end: 83
orientation: inline

U085O11+D0
type: ComponentDefinition
persistent identity: BBa I732402
display id: BBa_I732402
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member (U085O11+D062NUL)
creator: Zhan Jian
created: 2007-09-20T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1572
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Might be later edited.
mutable notes: Might be later edited.
mutable provenance: Might be later edited.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: consensus elements
sequence annotation: +1
sequence annotation: O1
sequence: https://synbiohub.org/public/igem/BBa_I732402_sequence/1

consensus elements
type: SequenceAnnotation
persistent identity: annotation1944539
display id: annotation1944539
version: 1
top level: U085O11+D0
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I732402/annotation1944539/range1944539/1

https://synbiohub.org/public/igem/BBa_I732402/annotation1944539/range1944539/1
type: Range
persistent identity: range1944539
display id: range1944539
version: 1
direction: direction/forward
top level: U085O11+D0
start: 69
end: 105
orientation: inline

+1
type: SequenceAnnotation
persistent identity: annotation1944541
display id: annotation1944541
version: 1
top level: U085O11+D0
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_I732402/annotation1944541/range1944541/1

https://synbiohub.org/public/igem/BBa_I732402/annotation1944541/range1944541/1
type: Range
persistent identity: range1944541
display id: range1944541
version: 1
direction: direction/forward
top level: U085O11+D0
start: 105
end: 105
orientation: inline

O1
type: SequenceAnnotation
persistent identity: annotation1944540
display id: annotation1944540
version: 1
top level: U085O11+D0
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_I732402/annotation1944540/range1944540/1

https://synbiohub.org/public/igem/BBa_I732402/annotation1944540/range1944540/1
type: Range
persistent identity: range1944540
display id: range1944540
version: 1
direction: direction/forward
top level: U085O11+D0
start: 1
end: 20
orientation: inline

U085O11+D0
type: ComponentDefinition
persistent identity: BBa I732421
display id: BBa_I732421
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member (U085O11+D026O22)
creator: Zhan Jian
created: 2007-10-02T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1592
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Our first NOR logical gate
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732421_sequence/1

BBa_K091105
type: ComponentDefinition
persistent identity: BBa K091105
display id: BBa_K091105
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pTet/Mnt Hybrid Promoter
creator: Robert Cool
created: 2008-06-18T11:00:00Z
modified: 2015-05-08T01:08:37Z
discontinued: false
dominant: false
experience: experience/None
group u list: _191_
m user id: 0
owner id: 3080
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This promoter is a modified version of the Mnt promoter that is also responsive to TetR. The promoter should be repressed by Mnt repressor. It should also be repressed by TetR, and in the absence of Mnt repressor, should be induced by aTc.
mutable notes: Mnt repressor binds as a tetramer to two half-operator sites. Introduction of TetR binding sites between the Mnt promoter -10 sequence and the start site for transcription should allow for repression by TetR. Accordingly, the hybrid promoter was designed by 1) Remove all bases of mnt promoter 3??? to -10: gagtcgtattaattt will be replaced by tccctatcagtgata, and 2) truncate the second half of ptet -10 and mutate -35 so that it function to bind tetR (replace ttgaca with actgta), but is not a promoter, and 3) place the tetR1 and tetR2 binding sites 3??? to truncated mnt -10 sequence.
mutable provenance: This part comes from the Mnt promoter and the TetR promoter
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: Mnt
sequence annotation: tetR2
sequence annotation: -10
sequence annotation: tetR1
sequence: https://synbiohub.org/public/igem/BBa_K091105_sequence/1

Mnt
type: SequenceAnnotation
persistent identity: annotation1963742
display id: annotation1963742
version: 1
top level: BBa_K091105
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K091105/annotation1963742/range1963742/1

https://synbiohub.org/public/igem/BBa_K091105/annotation1963742/range1963742/1
type: Range
persistent identity: range1963742
display id: range1963742
version: 1
direction: direction/forward
top level: BBa_K091105
start: 1
end: 52
orientation: inline

tetR2
type: SequenceAnnotation
persistent identity: annotation1963741
display id: annotation1963741
version: 1
top level: BBa_K091105
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K091105/annotation1963741/range1963741/1

https://synbiohub.org/public/igem/BBa_K091105/annotation1963741/range1963741/1
type: Range
persistent identity: range1963741
display id: range1963741
version: 1
direction: direction/forward
top level: BBa_K091105
start: 78
end: 98
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1963739
display id: annotation1963739
version: 1
top level: BBa_K091105
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K091105/annotation1963739/range1963739/1

https://synbiohub.org/public/igem/BBa_K091105/annotation1963739/range1963739/1
type: Range
persistent identity: range1963739
display id: range1963739
version: 1
direction: direction/forward
top level: BBa_K091105
start: 47
end: 52
orientation: inline

tetR1
type: SequenceAnnotation
persistent identity: annotation1963740
display id: annotation1963740
version: 1
top level: BBa_K091105
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K091105/annotation1963740/range1963740/1

https://synbiohub.org/public/igem/BBa_K091105/annotation1963740/range1963740/1
type: Range
persistent identity: range1963740
display id: range1963740
version: 1
direction: direction/forward
top level: BBa_K091105
start: 53
end: 71
orientation: inline

BBa_M13102
type: ComponentDefinition
persistent identity: BBa M13102
display id: BBa_M13102
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: M13K07 gene II promoter
creator: Natalie Kuldell
created: 2006-12-15T12:00:00Z
modified: 2015-05-08T01:13:55Z
discontinued: false
dominant: true
experience: experience/None
group u list: _45_
m user id: 0
owner id: 314
owning group id: 1
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This part is from the bacteriophage M13 genome. It directs transcription of M13 gene II (BBa_M13502, BBa_M13002). Promoter identified in Gene (1980) 11:129-148 based on -10,-35 homology and fragment of genome able to bind RNAP.
mutable notes: none
mutable provenance: New England Biolabs M13K07. Sequenced in 2002 by L. Panganaban.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_M13102_sequence/1

BBa_K427003
type: ComponentDefinition
persistent identity: BBa K427003
display id: BBa_K427003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Pm promoter of Mu bacteriophage
creator: Jan Marte Contreras Ortiz
created: 2010-10-21T11:00:00Z
modified: 2015-05-08T01:12:29Z
discontinued: false
dominant: false
group u list: _544_
m user id: 0
owner id: 6602
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Pm is the middle promoter of the Mu bacteriophage. It is in charge of the transcription of the activator protein C. Pm is activated by a product of the early transcription, protein Mor. It can be used to build a sensitivity tuner alongside this protein, this construct can be used to increase the amount of POPS in a certain genetic construction.
mutable notes: No special considerations
mutable provenance: Synthetized from the reported sequence of Pm promoter from Mu bacteriophage.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: Pm
sequence: https://synbiohub.org/public/igem/BBa_K427003_sequence/1

Pm
type: SequenceAnnotation
persistent identity: annotation2092367
display id: annotation2092367
version: 1
top level: BBa_K427003
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K427003/annotation2092367/range2092367/1

https://synbiohub.org/public/igem/BBa_K427003/annotation2092367/range2092367/1
type: Range
persistent identity: range2092367
display id: range2092367
version: 1
direction: direction/forward
top level: BBa_K427003
start: 1
end: 71
orientation: inline

pBad/araC
type: ComponentDefinition
persistent identity: BBa I0500
display id: BBa_I0500
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Inducible pBad/araC promoter
creator: Sri Kosuri
created: 2003-12-05T12:00:00Z
modified: 2015-08-31T04:07:29Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: PC
sequence annotation: Pbad
sequence annotation: AraC
sequence: https://synbiohub.org/public/igem/BBa_I0500_sequence/1

PC
type: SequenceAnnotation
persistent identity: annotation1498842
display id: annotation1498842
version: 1
top level: pBad/araC
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I0500/annotation1498842/range1498842/1

https://synbiohub.org/public/igem/BBa_I0500/annotation1498842/range1498842/1
type: Range
persistent identity: range1498842
display id: range1498842
version: 1
direction: direction/forward
top level: pBad/araC
start: 1043
end: 1114
orientation: inline

Pbad
type: SequenceAnnotation
persistent identity: annotation1498841
display id: annotation1498841
version: 1
top level: pBad/araC
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I0500/annotation1498841/range1498841/1

https://synbiohub.org/public/igem/BBa_I0500/annotation1498841/range1498841/1
type: Range
persistent identity: range1498841
display id: range1498841
version: 1
direction: direction/forward
top level: pBad/araC
start: 1080
end: 1210
orientation: inline

AraC
type: SequenceAnnotation
persistent identity: annotation1498843
display id: annotation1498843
version: 1
top level: pBad/araC
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I0500/annotation1498843/range1498843/1

https://synbiohub.org/public/igem/BBa_I0500/annotation1498843/range1498843/1
type: Range
persistent identity: range1498843
display id: range1498843
version: 1
direction: direction/forward
top level: pBad/araC
start: 1
end: 1079
orientation: inline

BBa_K1585110
type: ComponentDefinition
persistent identity: BBa K1585110
display id: BBa_K1585110
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Anderson Promoter with lacI binding site
creator: Tobias Schwanemann
created: 2015-08-31T11:00:00Z
modified: 2015-09-01T02:09:56Z
discontinued: false
dominant: false
experience: experience/Fails
group u list: _2002_
m user id: 24474
owner id: 24474
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Promoters of the Anderson Promoter Collection with lacI binding site derived from [http://parts.igem.org/Part:BBa_R0010 R_0010] to make them [http://openwetware.org/wiki/IPTG IPTG] iducible.
mutable notes: We choosed lacI-binding-site of part BBa_R0010 because we assumed older parts would be more reliable. The part is shipped in backbone [http://parts.igem.org/Part:BBa_J61002 J61002] because the functionality is directly visible by adding IPTG. RFP ([http://parts.igem.org/Part:BBa_E1010 E1010]) should get expressed then.
mutable provenance: Assambly of [http://parts.igem.org/Part:BBa_J23106 J23106] and lacI-binding-site of [http://parts.igem.org/Part:BBa_R0010 R_0010] The "lacI binding site" of Part [http://parts.igem.org/Part:BBa_R0010 R_0010] differs from lacO from Part [http://parts.igem.org/Part:BBa_J33207 BBa_J33207].
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
component: BBa_K1585999
component: BBa_J23110
sequence annotation: BBa_J23110
sequence annotation: BBa_K1585999
sequence: https://synbiohub.org/public/igem/BBa_K1585110_sequence/1

BBa_K1585999
type: Component
persistent identity: component2441701
display id: component2441701
version: 1
top level: BBa_K1585110
access: public
definition: https://synbiohub.org/public/igem/BBa_K1585999/1

BBa_J23110
type: Component
persistent identity: component2441699
display id: component2441699
version: 1
top level: BBa_K1585110
access: public
definition: BBa_J23110

BBa_J23110
type: SequenceAnnotation
persistent identity: annotation2441699
display id: annotation2441699
version: 1
top level: BBa_K1585110
location: https://synbiohub.org/public/igem/BBa_K1585110/annotation2441699/range2441699/1
component: BBa_J23110

https://synbiohub.org/public/igem/BBa_K1585110/annotation2441699/range2441699/1
type: Range
persistent identity: range2441699
display id: range2441699
version: 1
direction: direction/reverse
top level: BBa_K1585110
start: 1
end: 35
orientation: inline

BBa_K1585999
type: SequenceAnnotation
persistent identity: annotation2441701
display id: annotation2441701
version: 1
top level: BBa_K1585110
location: https://synbiohub.org/public/igem/BBa_K1585110/annotation2441701/range2441701/1
component: BBa_K1585999

https://synbiohub.org/public/igem/BBa_K1585110/annotation2441701/range2441701/1
type: Range
persistent identity: range2441701
display id: range2441701
version: 1
direction: direction/reverse
top level: BBa_K1585110
start: 44
end: 78
orientation: inline

BBa_J23104
type: ComponentDefinition
persistent identity: BBa J23104
display id: BBa_J23104
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: constitutive promoter family member
creator: John Anderson
created: 2006-08-03T11:00:00Z
modified: 2015-08-31T04:08:40Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: true
mutable description: replace later
mutable notes: N/A
mutable provenance: isolated from library of promoters
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J23104_sequence/1

Bla
type: ComponentDefinition
persistent identity: BBa I14018
display id: BBa_I14018
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: P(Bla)
creator: Vikram Vijayan, Allen Hsu, Lawrence Fomundam
created: 2004-08-01T11:00:00Z
modified: 2015-08-31T04:07:37Z
discontinued: false
dominant: false
experience: experience/None
group u list: _4_
m user id: 0
owner id: 171
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: P(Bla), Medium Transcription
mutable provenance: Plasmid pUC19
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: P(Bla)
sequence annotation: -10
sequence annotation: -35
sequence: https://synbiohub.org/public/igem/BBa_I14018_sequence/1

P(Bla)
type: SequenceAnnotation
persistent identity: annotation1018027
display id: annotation1018027
version: 1
top level: Bla
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_I14018/annotation1018027/range1018027/1

https://synbiohub.org/public/igem/BBa_I14018/annotation1018027/range1018027/1
type: Range
persistent identity: range1018027
display id: range1018027
version: 1
direction: direction/forward
top level: Bla
start: 1
end: 35
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1027016
display id: annotation1027016
version: 1
top level: Bla
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I14018/annotation1027016/range1027016/1

https://synbiohub.org/public/igem/BBa_I14018/annotation1027016/range1027016/1
type: Range
persistent identity: range1027016
display id: range1027016
version: 1
direction: direction/forward
top level: Bla
start: 25
end: 30
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1027020
display id: annotation1027020
version: 1
top level: Bla
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I14018/annotation1027020/range1027020/1

https://synbiohub.org/public/igem/BBa_I14018/annotation1027020/range1027020/1
type: Range
persistent identity: range1027020
display id: range1027020
version: 1
direction: direction/forward
top level: Bla
start: 4
end: 9
orientation: inline

Zif23
type: ComponentDefinition
persistent identity: BBa R2001
display id: BBa_R2001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter, Zif23 regulated, test: after
creator: Ronny Krashinsky
created: 2004-01-29T12:00:00Z
modified: 2015-05-08T01:14:15Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Promoter with standard -10 and -35 sites. A symmetric Zif23 dimer target site is after the -10 site.
mutable notes: consensus sequences based on [Jensen98].
mutable provenance: synthetic
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -10
sequence annotation: Zif23
sequence annotation: (+1)
sequence annotation: consensus
sequence annotation: -35
sequence: https://synbiohub.org/public/igem/BBa_R2001_sequence/1

-10
type: SequenceAnnotation
persistent identity: annotation318133
display id: annotation318133
version: 1
top level: Zif23
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R2001/annotation318133/range318133/1

https://synbiohub.org/public/igem/BBa_R2001/annotation318133/range318133/1
type: Range
persistent identity: range318133
display id: range318133
version: 1
direction: direction/forward
top level: Zif23
start: 33
end: 38
orientation: inline

Zif23
type: SequenceAnnotation
persistent identity: annotation318136
display id: annotation318136
version: 1
top level: Zif23
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R2001/annotation318136/range318136/1

https://synbiohub.org/public/igem/BBa_R2001/annotation318136/range318136/1
type: Range
persistent identity: range318136
display id: range318136
version: 1
direction: direction/forward
top level: Zif23
start: 39
end: 52
orientation: inline

(+1)
type: SequenceAnnotation
persistent identity: annotation318134
display id: annotation318134
version: 1
top level: Zif23
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_R2001/annotation318134/range318134/1

https://synbiohub.org/public/igem/BBa_R2001/annotation318134/range318134/1
type: Range
persistent identity: range318134
display id: range318134
version: 1
direction: direction/forward
top level: Zif23
start: 45
end: 45
orientation: inline

consensus
type: SequenceAnnotation
persistent identity: annotation318135
display id: annotation318135
version: 1
top level: Zif23
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_R2001/annotation318135/range318135/1

https://synbiohub.org/public/igem/BBa_R2001/annotation318135/range318135/1
type: Range
persistent identity: range318135
display id: range318135
version: 1
direction: direction/forward
top level: Zif23
start: 1
end: 9
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation318132
display id: annotation318132
version: 1
top level: Zif23
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R2001/annotation318132/range318132/1

https://synbiohub.org/public/igem/BBa_R2001/annotation318132/range318132/1
type: Range
persistent identity: range318132
display id: range318132
version: 1
direction: direction/forward
top level: Zif23
start: 10
end: 15
orientation: inline

consensus
type: SequenceAnnotation
persistent identity: annotation318142
display id: annotation318142
version: 1
top level: Zif23
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_R2001/annotation318142/range318142/1

https://synbiohub.org/public/igem/BBa_R2001/annotation318142/range318142/1
type: Range
persistent identity: range318142
display id: range318142
version: 1
direction: direction/forward
top level: Zif23
start: 30
end: 32
orientation: inline

BBa_K101000
type: ComponentDefinition
persistent identity: BBa K101000
display id: BBa_K101000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Dual-Repressed Promoter for p22 mnt and TetR
creator: Bennett Swiniarski
created: 2008-07-06T11:00:00Z
modified: 2015-05-08T01:08:41Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _205_
m user id: 0
owner id: 3363
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This part is a promoter that is repressed by both p22 mnt and TetR. It can be used to control the response of any gene that follows this promoter. It will be repressed by the presence of either p22 mnt or TetR.
mutable notes: TetR usually binds to two operator sites, one located upstream from -35 and one located between -35 and -10. However, p22 mnt also has two operator sites, one between -35 and -10 and another downstream from -10. Since each had an operator site in between -35 and -10, we had to eliminate the TetR operator site in order to allow the promoter to work. TetR was picked because it has a stronger binding than p22 mnt, and so would be more able to use only one site to bind.
mutable provenance: This part was designed by combining sequences from parts C0040 (TetR promoter) and C0072 (p22 mnt promoter).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K101000_sequence/1

BBa_S04509
type: ComponentDefinition
persistent identity: BBa S04509
display id: BBa_S04509
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: R0010:B0034
creator: Jacky, Fong Chuen, Loo
created: 2010-10-19T11:00:00Z
modified: 2015-05-08T01:14:41Z
discontinued: false
dominant: false
group u list: _9_
m user id: 0
owner id: 6123
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_R0010
component: BBa_B0034
sequence annotation: BBa_B0034
sequence annotation: BBa_R0010
sequence: https://synbiohub.org/public/igem/BBa_S04509_sequence/1

BBa_R0010
type: Component
persistent identity: component2090735
display id: component2090735
version: 1
top level: BBa_S04509
access: public
definition: LacI

BBa_B0034
type: Component
persistent identity: component2090743
display id: component2090743
version: 1
top level: BBa_S04509
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2090743
display id: annotation2090743
version: 1
top level: BBa_S04509
location: https://synbiohub.org/public/igem/BBa_S04509/annotation2090743/range2090743/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_S04509/annotation2090743/range2090743/1
type: Range
persistent identity: range2090743
display id: range2090743
version: 1
direction: direction/reverse
top level: BBa_S04509
start: 209
end: 220
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation2090735
display id: annotation2090735
version: 1
top level: BBa_S04509
location: https://synbiohub.org/public/igem/BBa_S04509/annotation2090735/range2090735/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_S04509/annotation2090735/range2090735/1
type: Range
persistent identity: range2090735
display id: range2090735
version: 1
direction: direction/forward
top level: BBa_S04509
start: 1
end: 200
orientation: inline

cI TP901
type: ComponentDefinition
persistent identity: BBa R0075
display id: BBa_R0075
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter (TP901 cI regulated)
creator: crackdots
created: 2004-01-27T12:00:00Z
modified: 2015-05-08T01:14:15Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: cI repressor from phage TP901 infecting L. lactis
mutable notes: operator sites: pR and pL
mutable provenance: TP901-1
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: cI tp901 OR
sequence annotation: -10
sequence annotation: start
sequence annotation: cI tp901 OL
sequence annotation: -35
sequence: https://synbiohub.org/public/igem/BBa_R0075_sequence/1

cI tp901 OR
type: SequenceAnnotation
persistent identity: annotation302714
display id: annotation302714
version: 1
top level: cI TP901
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0075/annotation302714/range302714/1

https://synbiohub.org/public/igem/BBa_R0075/annotation302714/range302714/1
type: Range
persistent identity: range302714
display id: range302714
version: 1
direction: direction/forward
top level: cI TP901
start: 19
end: 57
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation319985
display id: annotation319985
version: 1
top level: cI TP901
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0075/annotation319985/range319985/1

https://synbiohub.org/public/igem/BBa_R0075/annotation319985/range319985/1
type: Range
persistent identity: range319985
display id: range319985
version: 1
direction: direction/forward
top level: cI TP901
start: 76
end: 82
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation320591
display id: annotation320591
version: 1
top level: cI TP901
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_R0075/annotation320591/range320591/1

https://synbiohub.org/public/igem/BBa_R0075/annotation320591/range320591/1
type: Range
persistent identity: range320591
display id: range320591
version: 1
direction: direction/forward
top level: cI TP901
start: 89
end: 89
orientation: inline

cI tp901 OL
type: SequenceAnnotation
persistent identity: annotation319984
display id: annotation319984
version: 1
top level: cI TP901
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0075/annotation319984/range319984/1

https://synbiohub.org/public/igem/BBa_R0075/annotation319984/range319984/1
type: Range
persistent identity: range319984
display id: range319984
version: 1
direction: direction/forward
top level: cI TP901
start: 85
end: 115
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation319983
display id: annotation319983
version: 1
top level: cI TP901
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0075/annotation319983/range319983/1

https://synbiohub.org/public/igem/BBa_R0075/annotation319983/range319983/1
type: Range
persistent identity: range319983
display id: range319983
version: 1
direction: direction/forward
top level: cI TP901
start: 53
end: 59
orientation: inline

lux pR-5
type: ComponentDefinition
persistent identity: BBa K658007
display id: BBa_K658007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: position 5 mutated promoter lux pR-5 (luxR & HSL regulated)
creator: Yidan Hu
created: 2011-09-30T11:00:00Z
modified: 2015-05-08T01:13:02Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _835_
m user id: 0
owner id: 10207
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: The position 5 mutated promoter lux pR-5 is a product of site-directed mutagenesis of promoter lux pR (BBa_R0062). The promoter lux pR is based on the Vibrio fischeri quorum sensing gene promoters. The position 5 mutated promoter lux pR-5 gives a higher expression of downstream genes in an autoinduction system. In another word, the lux pR-5 has a higher efficiency than the lux pR. Similar to promoter lux pR, the expression is up-regulated by the action of LuxR protein combined with the signal molecule HSL.
mutable notes: By PCR method the G residue of position 5 was changed to a C based on the sequence of the promoter lux pR(BBa_R0062).
mutable provenance: The promoter lux pR (BBa_R0062).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: LuxR/HSL
sequence annotation: -35
sequence annotation: start
sequence annotation: K658007
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_K658007_sequence/1

LuxR/HSL
type: SequenceAnnotation
persistent identity: annotation2148169
display id: annotation2148169
version: 1
top level: lux pR-5
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K658007/annotation2148169/range2148169/1

https://synbiohub.org/public/igem/BBa_K658007/annotation2148169/range2148169/1
type: Range
persistent identity: range2148169
display id: range2148169
version: 1
direction: direction/forward
top level: lux pR-5
start: 1
end: 20
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2148170
display id: annotation2148170
version: 1
top level: lux pR-5
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K658007/annotation2148170/range2148170/1

https://synbiohub.org/public/igem/BBa_K658007/annotation2148170/range2148170/1
type: Range
persistent identity: range2148170
display id: range2148170
version: 1
direction: direction/forward
top level: lux pR-5
start: 20
end: 25
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2148167
display id: annotation2148167
version: 1
top level: lux pR-5
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K658007/annotation2148167/range2148167/1

https://synbiohub.org/public/igem/BBa_K658007/annotation2148167/range2148167/1
type: Range
persistent identity: range2148167
display id: range2148167
version: 1
direction: direction/forward
top level: lux pR-5
start: 53
end: 53
orientation: inline

K658007
type: SequenceAnnotation
persistent identity: annotation2148168
display id: annotation2148168
version: 1
top level: lux pR-5
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K658007/annotation2148168/range2148168/1

https://synbiohub.org/public/igem/BBa_K658007/annotation2148168/range2148168/1
type: Range
persistent identity: range2148168
display id: range2148168
version: 1
direction: direction/forward
top level: lux pR-5
start: 1
end: 55
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2148171
display id: annotation2148171
version: 1
top level: lux pR-5
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K658007/annotation2148171/range2148171/1

https://synbiohub.org/public/igem/BBa_K658007/annotation2148171/range2148171/1
type: Range
persistent identity: range2148171
display id: range2148171
version: 1
direction: direction/forward
top level: lux pR-5
start: 42
end: 47
orientation: inline

U097NUL+D0
type: ComponentDefinition
persistent identity: BBa I732412
display id: BBa_I732412
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member (U097NUL+D062O22)
creator: Zhan Jian
created: 2007-09-22T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1572
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: To be later edited if time permits.
mutable notes: To be later edited if time permits.
mutable provenance: To be later edited if time permits.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: consensus elements
sequence annotation: O2
sequence annotation: +1
sequence: https://synbiohub.org/public/igem/BBa_I732412_sequence/1

consensus elements
type: SequenceAnnotation
persistent identity: annotation1944564
display id: annotation1944564
version: 1
top level: U097NUL+D0
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I732412/annotation1944564/range1944564/1

https://synbiohub.org/public/igem/BBa_I732412/annotation1944564/range1944564/1
type: Range
persistent identity: range1944564
display id: range1944564
version: 1
direction: direction/forward
top level: U097NUL+D0
start: 61
end: 137
orientation: inline

O2
type: SequenceAnnotation
persistent identity: annotation1944565
display id: annotation1944565
version: 1
top level: U097NUL+D0
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_I732412/annotation1944565/range1944565/1

https://synbiohub.org/public/igem/BBa_I732412/annotation1944565/range1944565/1
type: Range
persistent identity: range1944565
display id: range1944565
version: 1
direction: direction/forward
top level: U097NUL+D0
start: 158
end: 177
orientation: inline

+1
type: SequenceAnnotation
persistent identity: annotation1944566
display id: annotation1944566
version: 1
top level: U097NUL+D0
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_I732412/annotation1944566/range1944566/1

https://synbiohub.org/public/igem/BBa_I732412/annotation1944566/range1944566/1
type: Range
persistent identity: range1944566
display id: range1944566
version: 1
direction: direction/forward
top level: U097NUL+D0
start: 137
end: 137
orientation: inline

BBa_J23111
type: ComponentDefinition
persistent identity: BBa J23111
display id: BBa_J23111
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: constitutive promoter family member
creator: John Anderson
created: 2006-08-16T11:00:00Z
modified: 2015-08-31T04:08:40Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: true
mutable description: Later
mutable notes: N/A
mutable provenance: Later
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J23111_sequence/1

BBa_K1585101
type: ComponentDefinition
persistent identity: BBa K1585101
display id: BBa_K1585101
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Anderson Promoter with lacI binding site
creator: Tobias Schwanemann
created: 2015-08-23T11:00:00Z
modified: 2015-09-01T03:27:19Z
discontinued: false
dominant: false
experience: experience/Fails
group u list: _2002_
m user id: 24474
owner id: 24474
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Promoters of the Anderson Promoter Collection with lacI binding site derived from [http://parts.igem.org/Part:BBa_R0010 R_0010] to make them [http://openwetware.org/wiki/IPTG IPTG] iducible.
mutable notes: The "lacI binding site" of Part [http://parts.igem.org/Part:BBa_R0010 R_0010] differs from lacO from Part [http://parts.igem.org/Part:BBa_J33207 BBa_J33207] although they should be same. We choosed part BBa_R0010 because we assumed older parts would be reliable.
mutable provenance: Assambly of [http://parts.igem.org/Part:BBa_J23100 J23100] and lacI-binding-site of [http://parts.igem.org/Part:BBa_R0010 R_0010]
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
component: BBa_K1585999
component: BBa_J23101
sequence annotation: BBa_K1585999
sequence annotation: BBa_J23101
sequence: https://synbiohub.org/public/igem/BBa_K1585101_sequence/1

BBa_K1585999
type: Component
persistent identity: component2436907
display id: component2436907
version: 1
top level: BBa_K1585101
access: public
definition: https://synbiohub.org/public/igem/BBa_K1585999/1

BBa_J23101
type: Component
persistent identity: component2436906
display id: component2436906
version: 1
top level: BBa_K1585101
access: public
definition: BBa_J23101

BBa_K1585999
type: SequenceAnnotation
persistent identity: annotation2436907
display id: annotation2436907
version: 1
top level: BBa_K1585101
location: https://synbiohub.org/public/igem/BBa_K1585101/annotation2436907/range2436907/1
component: BBa_K1585999

https://synbiohub.org/public/igem/BBa_K1585101/annotation2436907/range2436907/1
type: Range
persistent identity: range2436907
display id: range2436907
version: 1
direction: direction/reverse
top level: BBa_K1585101
start: 44
end: 78
orientation: inline

BBa_J23101
type: SequenceAnnotation
persistent identity: annotation2436906
display id: annotation2436906
version: 1
top level: BBa_K1585101
location: https://synbiohub.org/public/igem/BBa_K1585101/annotation2436906/range2436906/1
component: BBa_J23101

https://synbiohub.org/public/igem/BBa_K1585101/annotation2436906/range2436906/1
type: Range
persistent identity: range2436906
display id: range2436906
version: 1
direction: direction/reverse
top level: BBa_K1585101
start: 1
end: 35
orientation: inline

BBa_I746364
type: ComponentDefinition
persistent identity: BBa I746364
display id: BBa_I746364
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Psid promoter from P4 phage
creator: Stefan Milde
created: 2007-09-10T11:00:00Z
modified: 2015-08-31T04:08:04Z
discontinued: false
dominant: false
group u list: _116_
m user id: 0
owner id: 2122
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This is the Psid promoter taken from the P4 phage genome. It is an inducible promoter that is activated by a class of activators, including P2 ogr (I746350), PSP3 pag (I746351) and phiR73 delta (I746352). These different activators should cause different levels of activity of the Psid promoter.
mutable notes: no special considerations
mutable provenance: P4 phage genome
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: Psid
sequence: https://synbiohub.org/public/igem/BBa_I746364_sequence/1

Psid
type: SequenceAnnotation
persistent identity: annotation1943787
display id: annotation1943787
version: 1
top level: BBa_I746364
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I746364/annotation1943787/range1943787/1

https://synbiohub.org/public/igem/BBa_I746364/annotation1943787/range1943787/1
type: Range
persistent identity: range1943787
display id: range1943787
version: 1
direction: direction/forward
top level: BBa_I746364
start: 1
end: 93
orientation: inline

BBa_M13110
type: ComponentDefinition
persistent identity: BBa M13110
display id: BBa_M13110
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: M13110
creator: Natalie Kuldell
created: 2006-12-15T12:00:00Z
modified: 2015-05-08T01:13:55Z
discontinued: false
dominant: false
group u list: _45_
m user id: 0
owner id: 314
owning group id: 1
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This part is from the bacteriophage M13 genome, bp 381-428 in M13K07. It directs transcription of M13 gene X (BBa_M13510, BBa_M13010). Its identity as a promoter was described in Gene (1980) 11:129-148 based on -10,-35 homology and fragment of genome able to bind RNAP.
mutable notes: none
mutable provenance: New England Biolabs M13K07. Sequenced in 2002 by L. Panganaban.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_M13110_sequence/1

Lux
type: ComponentDefinition
persistent identity: BBa I1051
display id: BBa_I1051
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Lux cassette right promoter
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:30Z
discontinued: false
dominant: true
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: The lux cassette of V. fischeri contains a left and a right promoter. The right promoter gives weak constitutive expression of downstream genes.This expression is up-regulated by the action of the Lux repressor, LuxR. Two molecules of LuxR protein form a complex with two molecules the signalling compound homoserine lactone (HSL). This complex binds to a palindromic site on the promoter, increasing the rate of transcription.In thisversion, a binding site for cI dimer has been added: binding to this site dominantly represses transcription.
mutable provenance: V. fishceri and phage lambda (courtesy of Ron Weiss)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -10
sequence annotation: LuxR/HSL
sequence annotation: cI (OR1)
sequence annotation: -35
sequence annotation: start
sequence annotation: BBa_I1051
sequence: https://synbiohub.org/public/igem/BBa_I1051_sequence/1

-10
type: SequenceAnnotation
persistent identity: annotation1938
display id: annotation1938
version: 1
top level: Lux
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I1051/annotation1938/range1938/1

https://synbiohub.org/public/igem/BBa_I1051/annotation1938/range1938/1
type: Range
persistent identity: range1938
display id: range1938
version: 1
direction: direction/forward
top level: Lux
start: 42
end: 47
orientation: inline

LuxR/HSL
type: SequenceAnnotation
persistent identity: annotation1936
display id: annotation1936
version: 1
top level: Lux
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I1051/annotation1936/range1936/1

https://synbiohub.org/public/igem/BBa_I1051/annotation1936/range1936/1
type: Range
persistent identity: range1936
display id: range1936
version: 1
direction: direction/forward
top level: Lux
start: 1
end: 20
orientation: inline

cI (OR1)
type: SequenceAnnotation
persistent identity: annotation1939
display id: annotation1939
version: 1
top level: Lux
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I1051/annotation1939/range1939/1

https://synbiohub.org/public/igem/BBa_I1051/annotation1939/range1939/1
type: Range
persistent identity: range1939
display id: range1939
version: 1
direction: direction/forward
top level: Lux
start: 52
end: 68
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1937
display id: annotation1937
version: 1
top level: Lux
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I1051/annotation1937/range1937/1

https://synbiohub.org/public/igem/BBa_I1051/annotation1937/range1937/1
type: Range
persistent identity: range1937
display id: range1937
version: 1
direction: direction/forward
top level: Lux
start: 20
end: 25
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation1940
display id: annotation1940
version: 1
top level: Lux
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_I1051/annotation1940/range1940/1

https://synbiohub.org/public/igem/BBa_I1051/annotation1940/range1940/1
type: Range
persistent identity: range1940
display id: range1940
version: 1
direction: direction/forward
top level: Lux
start: 53
end: 53
orientation: inline

BBa_I1051
type: SequenceAnnotation
persistent identity: annotation7059
display id: annotation7059
version: 1
top level: Lux
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I1051/annotation7059/range7059/1

https://synbiohub.org/public/igem/BBa_I1051/annotation7059/range7059/1
type: Range
persistent identity: range7059
display id: range7059
version: 1
direction: direction/forward
top level: Lux
start: 1
end: 68
orientation: inline

BBa_K266000
type: ComponentDefinition
persistent identity: BBa K266000
display id: BBa_K266000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PAI+LasR -> LuxI (AI)
creator: UNAM FCIENCIAS-CINVESTAV IRA
created: 2009-10-12T11:00:00Z
modified: 2015-05-08T01:11:43Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _363_
m user id: 0
owner id: 1955
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This biobrick has a promoter inducible by PAI+LasR (BBa_R0079)and produces LuxI enzyme (BBa_F1610). This enzyme produces 3OC6HSL (AI).
mutable notes: None
mutable provenance: The DNA of BBa_R0079 and BBa_F1610 biobricks where used.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Signalling
role: SO:0000804
component: BBa_F1610
component: BBa_R0079
sequence annotation: BBa_R0079
sequence annotation: BBa_F1610
sequence: https://synbiohub.org/public/igem/BBa_K266000_sequence/1

BBa_F1610
type: Component
persistent identity: component2256062
display id: component2256062
version: 1
top level: BBa_K266000
access: public
definition: https://synbiohub.org/public/igem/BBa_F1610/1

BBa_R0079
type: Component
persistent identity: component2256049
display id: component2256049
version: 1
top level: BBa_K266000
access: public
definition: LasR+PAI

BBa_R0079
type: SequenceAnnotation
persistent identity: annotation2256049
display id: annotation2256049
version: 1
top level: BBa_K266000
location: https://synbiohub.org/public/igem/BBa_K266000/annotation2256049/range2256049/1
component: BBa_R0079

https://synbiohub.org/public/igem/BBa_K266000/annotation2256049/range2256049/1
type: Range
persistent identity: range2256049
display id: range2256049
version: 1
direction: direction/reverse
top level: BBa_K266000
start: 1
end: 157
orientation: inline

BBa_F1610
type: SequenceAnnotation
persistent identity: annotation2256062
display id: annotation2256062
version: 1
top level: BBa_K266000
location: https://synbiohub.org/public/igem/BBa_K266000/annotation2256062/range2256062/1
component: BBa_F1610

https://synbiohub.org/public/igem/BBa_K266000/annotation2256062/range2256062/1
type: Range
persistent identity: range2256062
display id: range2256062
version: 1
direction: direction/reverse
top level: BBa_K266000
start: 166
end: 963
orientation: inline

BBa_J23115
type: ComponentDefinition
persistent identity: BBa J23115
display id: BBa_J23115
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: constitutive promoter family member
creator: John Anderson
created: 2006-08-16T11:00:00Z
modified: 2015-08-31T04:08:40Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: true
mutable description: Later
mutable notes: N/A
mutable provenance: Later
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J23115_sequence/1

U073O11+D0
type: ComponentDefinition
persistent identity: BBa I732425
display id: BBa_I732425
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member (U073O11+D002O22)
creator: Zhan Jian
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: true
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732425_sequence/1

BBa_J64065
type: ComponentDefinition
persistent identity: BBa J64065
display id: BBa_J64065
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: cI repressed promoter
creator: Jeffrey J Tabor
created: 2007-05-12T11:00:00Z
modified: 2015-05-08T01:08:16Z
discontinued: false
dominant: false
experience: experience/Fails
group u list: _98_
m user id: 0
owner id: 88
owning group id: 98
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is a LuxR-30C6HSL-independent version of R0065. All DNA upstream of the -10 box is from the constitutive promoter J23119. All DNA downstream from the -10 box down is from R0065. This results in the replacement of the Lux box region for a constitutive promoter region and the maintenance of the two cI operators downstream of the transcription start site.
mutable notes: none
mutable provenance: J23119 + R0065
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -35
sequence annotation: +1
sequence annotation: -10
sequence annotation: OR2
sequence annotation: OR1
sequence: https://synbiohub.org/public/igem/BBa_J64065_sequence/1

-35
type: SequenceAnnotation
persistent identity: annotation1932681
display id: annotation1932681
version: 1
top level: BBa_J64065
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J64065/annotation1932681/range1932681/1

https://synbiohub.org/public/igem/BBa_J64065/annotation1932681/range1932681/1
type: Range
persistent identity: range1932681
display id: range1932681
version: 1
direction: direction/forward
top level: BBa_J64065
start: 1
end: 6
orientation: inline

+1
type: SequenceAnnotation
persistent identity: annotation1932685
display id: annotation1932685
version: 1
top level: BBa_J64065
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J64065/annotation1932685/range1932685/1

https://synbiohub.org/public/igem/BBa_J64065/annotation1932685/range1932685/1
type: Range
persistent identity: range1932685
display id: range1932685
version: 1
direction: direction/forward
top level: BBa_J64065
start: 35
end: 35
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1932682
display id: annotation1932682
version: 1
top level: BBa_J64065
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J64065/annotation1932682/range1932682/1

https://synbiohub.org/public/igem/BBa_J64065/annotation1932682/range1932682/1
type: Range
persistent identity: range1932682
display id: range1932682
version: 1
direction: direction/forward
top level: BBa_J64065
start: 24
end: 29
orientation: inline

OR2
type: SequenceAnnotation
persistent identity: annotation1932684
display id: annotation1932684
version: 1
top level: BBa_J64065
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_J64065/annotation1932684/range1932684/1

https://synbiohub.org/public/igem/BBa_J64065/annotation1932684/range1932684/1
type: Range
persistent identity: range1932684
display id: range1932684
version: 1
direction: direction/forward
top level: BBa_J64065
start: 58
end: 74
orientation: inline

OR1
type: SequenceAnnotation
persistent identity: annotation1932683
display id: annotation1932683
version: 1
top level: BBa_J64065
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_J64065/annotation1932683/range1932683/1

https://synbiohub.org/public/igem/BBa_J64065/annotation1932683/range1932683/1
type: Range
persistent identity: range1932683
display id: range1932683
version: 1
direction: direction/forward
top level: BBa_J64065
start: 34
end: 51
orientation: inline

BBa_J23151
type: ComponentDefinition
persistent identity: BBa J23151
display id: BBa_J23151
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: 1bp mutant from J23114
creator: Jason Kelly
created: 2008-02-11T12:00:00Z
modified: 2015-08-31T04:08:41Z
discontinued: false
dominant: false
group u list: _11_
m user id: 0
owner id: 2
owning group id: 84
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: .
mutable notes: .
mutable provenance: .
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J23151_sequence/1

BBa_S04209
type: ComponentDefinition
persistent identity: BBa S04209
display id: BBa_S04209
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: R0051:Q04121:B0034:C0079:B0015
creator: UNAM FCiencias-CINVESTAV IRA
created: 2009-06-02T11:00:00Z
modified: 2015-05-08T01:14:36Z
discontinued: false
dominant: true
experience: experience/None
group u list: _9_
m user id: 0
owner id: 1955
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_S04208
component: BBa_B0034
sequence annotation: BBa_S04208
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_S04209_sequence/1

BBa_S04208
type: Component
persistent identity: component2267293
display id: component2267293
version: 1
top level: BBa_S04209
access: public
definition: https://synbiohub.org/public/igem/BBa_S04208/1

BBa_B0034
type: Component
persistent identity: component2267295
display id: component2267295
version: 1
top level: BBa_S04209
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_S04208
type: SequenceAnnotation
persistent identity: annotation2267293
display id: annotation2267293
version: 1
top level: BBa_S04209
location: https://synbiohub.org/public/igem/BBa_S04209/annotation2267293/range2267293/1
component: BBa_S04208

https://synbiohub.org/public/igem/BBa_S04209/annotation2267293/range2267293/1
type: Range
persistent identity: range2267293
display id: range2267293
version: 1
direction: direction/reverse
top level: BBa_S04209
start: 1
end: 1414
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2267295
display id: annotation2267295
version: 1
top level: BBa_S04209
location: https://synbiohub.org/public/igem/BBa_S04209/annotation2267295/range2267295/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_S04209/annotation2267295/range2267295/1
type: Range
persistent identity: range2267295
display id: range2267295
version: 1
direction: direction/reverse
top level: BBa_S04209
start: 1423
end: 1434
orientation: inline

U049O11+D0
type: ComponentDefinition
persistent identity: BBa I732438
display id: BBa_I732438
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member (U049O11+D014O22)
creator: Zhan Jian
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732438_sequence/1

BBa_J64068
type: ComponentDefinition
persistent identity: BBa J64068
display id: BBa_J64068
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: increased strength R0051
creator: Jeffrey J Tabor
created: 2008-01-11T12:00:00Z
modified: 2015-05-08T01:08:17Z
discontinued: false
dominant: false
experience: experience/None
group u list: _98_
m user id: 0
owner id: 88
owning group id: 98
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: R0051 was modified to contain consensus -35 and -10 sequences. The CI operator OR1 was moved from it's original position (overlapping the -10) to between the -35 and -10. Fortuitously this results in the optimal (for high expression) 17bp spacing between the -35 and -10 sites.
mutable notes: none
mutable provenance: designed
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -35
sequence annotation: -10
sequence annotation: OR1
sequence: https://synbiohub.org/public/igem/BBa_J64068_sequence/1

-35
type: SequenceAnnotation
persistent identity: annotation1959539
display id: annotation1959539
version: 1
top level: BBa_J64068
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J64068/annotation1959539/range1959539/1

https://synbiohub.org/public/igem/BBa_J64068/annotation1959539/range1959539/1
type: Range
persistent identity: range1959539
display id: range1959539
version: 1
direction: direction/forward
top level: BBa_J64068
start: 15
end: 20
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1959541
display id: annotation1959541
version: 1
top level: BBa_J64068
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J64068/annotation1959541/range1959541/1

https://synbiohub.org/public/igem/BBa_J64068/annotation1959541/range1959541/1
type: Range
persistent identity: range1959541
display id: range1959541
version: 1
direction: direction/forward
top level: BBa_J64068
start: 38
end: 43
orientation: inline

OR1
type: SequenceAnnotation
persistent identity: annotation1959540
display id: annotation1959540
version: 1
top level: BBa_J64068
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_J64068/annotation1959540/range1959540/1

https://synbiohub.org/public/igem/BBa_J64068/annotation1959540/range1959540/1
type: Range
persistent identity: range1959540
display id: range1959540
version: 1
direction: direction/forward
top level: BBa_J64068
start: 21
end: 37
orientation: inline

lacI+pL
type: ComponentDefinition
persistent identity: BBa R0011
display id: BBa_R0011
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter (lacI regulated, lambda pL hybrid)
creator: Neelaksh Varshney, Grace Kenney, Daniel Shen, Samantha Sutton
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) The PLlac 0-1 promoter is a hybrid regulatory region consisting of the promoter P(L) of phage lambda with the cI binding sites replaced with lacO1. The hybrid design allows for strong promotion that can nevertheless be tightly repressed by LacI, the Lac inhibitor (i.e. repressor) (<bb_part>BBa_C0010</bb_part>) ([LUTZ97]). The activity of the promoter can be regulated over a >600-fold range by IPTG in E.Coli DH5-alpha-Z1 (same paper reference).
mutable notes: hybrid promoter design to create strong promoter that is, at the same time, highly repressible. note that the upstream operator installed in this hybrid is slightly different than the one in the original source (Lutz and Bujard, 1997). the most upstream operator region is slightly truncated in the represillator version, so that both operators in the hybrid are the same sequence. see references for details. also, the sequence has been truncated after the transcriptional start site. LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and increase transcription. This part is incompatible with environments containing lactose or lactose analogs.
mutable provenance: represillator of Elowitz and Leibler (2000)
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -35
sequence annotation: BBa_R0011
sequence annotation: lac O1
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R0011_sequence/1

-35
type: SequenceAnnotation
persistent identity: annotation2000
display id: annotation2000
version: 1
top level: lacI+pL
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0011/annotation2000/range2000/1

https://synbiohub.org/public/igem/BBa_R0011/annotation2000/range2000/1
type: Range
persistent identity: range2000
display id: range2000
version: 1
direction: direction/forward
top level: lacI+pL
start: 20
end: 25
orientation: inline

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation7064
display id: annotation7064
version: 1
top level: lacI+pL
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_R0011/annotation7064/range7064/1

https://synbiohub.org/public/igem/BBa_R0011/annotation7064/range7064/1
type: Range
persistent identity: range7064
display id: range7064
version: 1
direction: direction/forward
top level: lacI+pL
start: 1
end: 54
orientation: inline

lac O1
type: SequenceAnnotation
persistent identity: annotation2001
display id: annotation2001
version: 1
top level: lacI+pL
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_R0011/annotation2001/range2001/1

https://synbiohub.org/public/igem/BBa_R0011/annotation2001/range2001/1
type: Range
persistent identity: range2001
display id: range2001
version: 1
direction: direction/forward
top level: lacI+pL
start: 26
end: 42
orientation: inline

lac O1
type: SequenceAnnotation
persistent identity: annotation1999
display id: annotation1999
version: 1
top level: lacI+pL
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_R0011/annotation1999/range1999/1

https://synbiohub.org/public/igem/BBa_R0011/annotation1999/range1999/1
type: Range
persistent identity: range1999
display id: range1999
version: 1
direction: direction/forward
top level: lacI+pL
start: 3
end: 19
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2002
display id: annotation2002
version: 1
top level: lacI+pL
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0011/annotation2002/range2002/1

https://synbiohub.org/public/igem/BBa_R0011/annotation2002/range2002/1
type: Range
persistent identity: range2002
display id: range2002
version: 1
direction: direction/forward
top level: lacI+pL
start: 43
end: 48
orientation: inline

cI+luxR
type: ComponentDefinition
persistent identity: BBa R0065
display id: BBa_R0065
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter (lambda cI and luxR regulated -- hybrid)
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:15Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: cI repressor negatively regulates this promoter and LuxR activates its transcription.The effect of cI is dominant over LuxR. This part is based on the LuxR and cI repressor regulated hybrid promoter designed and tested by Ron Weiss. It requires the binding of two cI repressor dimers for maximal repression and contains two cI repressor binding sites namely, OR1 and OR2. This promoter is leaky in the sense that 'some' transcription is seen in the absence of both cI and LuxR. LuxI cI activity of promoter + + zero + - maximum - + zero - - leaky (no quantitative information)
mutable notes: This part was designed based on the LuxR and cI repressor regulated hybrid promoter tested by Ron Weiss and the LuxR-LuxICDABE sequence annotated by Tom Knight AF170104 .
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -10
sequence annotation: OR1 cI
sequence annotation: Lux Box
sequence annotation: -35
sequence annotation: lux p(R) start
sequence annotation: OR2 cI
sequence annotation: BBa_R0065
sequence: https://synbiohub.org/public/igem/BBa_R0065_sequence/1

-10
type: SequenceAnnotation
persistent identity: annotation1986781
display id: annotation1986781
version: 1
top level: cI+luxR
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0065/annotation1986781/range1986781/1

https://synbiohub.org/public/igem/BBa_R0065/annotation1986781/range1986781/1
type: Range
persistent identity: range1986781
display id: range1986781
version: 1
direction: direction/forward
top level: cI+luxR
start: 94
end: 97
orientation: inline

OR1 cI
type: SequenceAnnotation
persistent identity: annotation1986780
display id: annotation1986780
version: 1
top level: cI+luxR
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0065/annotation1986780/range1986780/1

https://synbiohub.org/public/igem/BBa_R0065/annotation1986780/range1986780/1
type: Range
persistent identity: range1986780
display id: range1986780
version: 1
direction: direction/forward
top level: cI+luxR
start: 81
end: 97
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1986776
display id: annotation1986776
version: 1
top level: cI+luxR
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0065/annotation1986776/range1986776/1

https://synbiohub.org/public/igem/BBa_R0065/annotation1986776/range1986776/1
type: Range
persistent identity: range1986776
display id: range1986776
version: 1
direction: direction/forward
top level: cI+luxR
start: 47
end: 52
orientation: inline

Lux Box
type: SequenceAnnotation
persistent identity: annotation1986775
display id: annotation1986775
version: 1
top level: cI+luxR
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0065/annotation1986775/range1986775/1

https://synbiohub.org/public/igem/BBa_R0065/annotation1986775/range1986775/1
type: Range
persistent identity: range1986775
display id: range1986775
version: 1
direction: direction/forward
top level: cI+luxR
start: 6
end: 25
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1986779
display id: annotation1986779
version: 1
top level: cI+luxR
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0065/annotation1986779/range1986779/1

https://synbiohub.org/public/igem/BBa_R0065/annotation1986779/range1986779/1
type: Range
persistent identity: range1986779
display id: range1986779
version: 1
direction: direction/forward
top level: cI+luxR
start: 71
end: 76
orientation: inline

lux p(R) start
type: SequenceAnnotation
persistent identity: annotation1986778
display id: annotation1986778
version: 1
top level: cI+luxR
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0065/annotation1986778/range1986778/1

https://synbiohub.org/public/igem/BBa_R0065/annotation1986778/range1986778/1
type: Range
persistent identity: range1986778
display id: range1986778
version: 1
direction: direction/forward
top level: cI+luxR
start: 58
end: 58
orientation: inline

OR2 cI
type: SequenceAnnotation
persistent identity: annotation1986777
display id: annotation1986777
version: 1
top level: cI+luxR
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0065/annotation1986777/range1986777/1

https://synbiohub.org/public/igem/BBa_R0065/annotation1986777/range1986777/1
type: Range
persistent identity: range1986777
display id: range1986777
version: 1
direction: direction/forward
top level: cI+luxR
start: 57
end: 73
orientation: inline

BBa_R0065
type: SequenceAnnotation
persistent identity: annotation1986774
display id: annotation1986774
version: 1
top level: cI+luxR
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_R0065/annotation1986774/range1986774/1

https://synbiohub.org/public/igem/BBa_R0065/annotation1986774/range1986774/1
type: Range
persistent identity: range1986774
display id: range1986774
version: 1
direction: direction/forward
top level: cI+luxR
start: 1
end: 97
orientation: inline

PenI
type: ComponentDefinition
persistent identity: BBa R0074
display id: BBa_R0074
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter (PenI regulated)
creator: crackdots
created: 2004-01-27T12:00:00Z
modified: 2015-05-08T01:14:15Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: PenP operator from Bacillus Lichenformis. Two operator sites are negatively regulated by PenI (C0074).
mutable notes: extends slightly past -50
mutable provenance: bacillus licheniformis
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: dimer left half
sequence annotation: -35
sequence annotation: -10
sequence annotation: PenI
sequence annotation: dimer right half
sequence: https://synbiohub.org/public/igem/BBa_R0074_sequence/1

dimer left half
type: SequenceAnnotation
persistent identity: annotation319788
display id: annotation319788
version: 1
top level: PenI
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0074/annotation319788/range319788/1

https://synbiohub.org/public/igem/BBa_R0074/annotation319788/range319788/1
type: Range
persistent identity: range319788
display id: range319788
version: 1
direction: direction/forward
top level: PenI
start: 31
end: 53
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation319211
display id: annotation319211
version: 1
top level: PenI
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0074/annotation319211/range319211/1

https://synbiohub.org/public/igem/BBa_R0074/annotation319211/range319211/1
type: Range
persistent identity: range319211
display id: range319211
version: 1
direction: direction/forward
top level: PenI
start: 23
end: 28
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation319212
display id: annotation319212
version: 1
top level: PenI
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0074/annotation319212/range319212/1

https://synbiohub.org/public/igem/BBa_R0074/annotation319212/range319212/1
type: Range
persistent identity: range319212
display id: range319212
version: 1
direction: direction/forward
top level: PenI
start: 46
end: 51
orientation: inline

PenI
type: SequenceAnnotation
persistent identity: annotation302713
display id: annotation302713
version: 1
top level: PenI
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_R0074/annotation302713/range302713/1

https://synbiohub.org/public/igem/BBa_R0074/annotation302713/range302713/1
type: Range
persistent identity: range302713
display id: range302713
version: 1
direction: direction/forward
top level: PenI
start: 1
end: 77
orientation: inline

dimer right half
type: SequenceAnnotation
persistent identity: annotation319789
display id: annotation319789
version: 1
top level: PenI
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0074/annotation319789/range319789/1

https://synbiohub.org/public/igem/BBa_R0074/annotation319789/range319789/1
type: Range
persistent identity: range319789
display id: range319789
version: 1
direction: direction/forward
top level: PenI
start: 55
end: 76
orientation: inline

pRecA
type: ComponentDefinition
persistent identity: BBa K093000
display id: BBa_K093000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pRecA with LexA binding site
creator: Julian Wiegelmann, Danielle Nash
created: 2008-10-25T11:00:00Z
modified: 2015-05-08T01:08:40Z
discontinued: false
dominant: false
experience: experience/None
group u list: _247_
m user id: 0
owner id: 3630
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This is a pRecA promoter with LexA binding sites. LexA is cleaved by RecA when RecA is upregulated due to induction of the SOS response triggered by single stranded DNA. For this part to work it must be in a RecA+ strain of E. coli such as TG1
mutable notes: How far past the start site of transcription do you need to go in order for the promoter to drive transcription? The promoter has been confirmed to drive transcription in a regular DH5alpha strain, so it the answer to the question is, 3 bp before the suffix, maybe less. The LexA binding site is in the core region of the promoter (between the -35 and -10 sigma 70 binding sites). According to Robert Sidney Cox III and colleagues in their paper "Programming ene expression with combinatorial promoters, the core region is the most effective region for binding of repressor molecules.
mutable provenance: synthetic. Oligonucleotides were designed to form the double stranded molecule with sticky ends for direct ligation into a biobrick vector.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -10
sequence annotation: LexA binding
sequence annotation: -35
sequence: https://synbiohub.org/public/igem/BBa_K093000_sequence/1

-10
type: SequenceAnnotation
persistent identity: annotation1989775
display id: annotation1989775
version: 1
top level: pRecA
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K093000/annotation1989775/range1989775/1

https://synbiohub.org/public/igem/BBa_K093000/annotation1989775/range1989775/1
type: Range
persistent identity: range1989775
display id: range1989775
version: 1
direction: direction/forward
top level: pRecA
start: 30
end: 39
orientation: inline

LexA binding
type: SequenceAnnotation
persistent identity: annotation1989779
display id: annotation1989779
version: 1
top level: pRecA
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K093000/annotation1989779/range1989779/1

https://synbiohub.org/public/igem/BBa_K093000/annotation1989779/range1989779/1
type: Range
persistent identity: range1989779
display id: range1989779
version: 1
direction: direction/forward
top level: pRecA
start: 15
end: 35
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1989749
display id: annotation1989749
version: 1
top level: pRecA
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K093000/annotation1989749/range1989749/1

https://synbiohub.org/public/igem/BBa_K093000/annotation1989749/range1989749/1
type: Range
persistent identity: range1989749
display id: range1989749
version: 1
direction: direction/forward
top level: pRecA
start: 11
end: 17
orientation: inline

U049O11+D0
type: ComponentDefinition
persistent identity: BBa I732440
display id: BBa_I732440
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member (U049O11+D038O22)
creator: Zhan Jian
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732440_sequence/1

BBa_J22126
type: ComponentDefinition
persistent identity: BBa J22126
display id: BBa_J22126
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Rec A (SOS) promoter
creator: Krithiga Sankaran
created: 2006-09-05T11:00:00Z
modified: 2015-08-31T04:08:38Z
discontinued: false
dominant: false
group u list: _70_
m user id: 0
owner id: 1038
owning group id: 70
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: RecA (SOS)promoter.
mutable notes: The Rec A(SOS) promoter has been PCR amplified from the chromosomal DNA from MG1655(Ecoli).
mutable provenance: NA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J22126_sequence/1

BBa_K137087
type: ComponentDefinition
persistent identity: BBa K137087
display id: BBa_K137087
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: optimized (TA) repeat constitutive promoter with 17 bp between -10 and -35 elements
creator: Allen Lin
created: 2008-08-20T11:00:00Z
modified: 2015-05-08T01:10:10Z
discontinued: false
dominant: false
experience: experience/None
group u list: _187_
m user id: 0
owner id: 3112
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: optimized (TA) repeat constitutive promoter with 17 bp between -10 and -35 elements
mutable notes: None
mutable provenance: PCR
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K137087_sequence/1

LasR+PAI
type: ComponentDefinition
persistent identity: BBa R0079
display id: BBa_R0079
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter (LasR & PAI regulated)
creator: Alvin Carter Powers (Fighting Darwins)
created: 2004-01-27T12:00:00Z
modified: 2015-05-08T01:14:15Z
discontinued: false
dominant: true
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Binding region for LasR protein (positive regulation)
mutable provenance: "Analysis of the Pseudomonas aeruginosa Elastase (lasB) Regulatory Region". Lynn Rust, Everett Pesci, and Barbara Iglewski
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: OP2
sequence annotation: OP1
sequence annotation: -10
sequence annotation: -35
sequence: https://synbiohub.org/public/igem/BBa_R0079_sequence/1

OP2
type: SequenceAnnotation
persistent identity: annotation300985
display id: annotation300985
version: 1
top level: LasR+PAI
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0079/annotation300985/range300985/1

https://synbiohub.org/public/igem/BBa_R0079/annotation300985/range300985/1
type: Range
persistent identity: range300985
display id: range300985
version: 1
direction: direction/forward
top level: LasR+PAI
start: 46
end: 63
orientation: inline

OP1
type: SequenceAnnotation
persistent identity: annotation300992
display id: annotation300992
version: 1
top level: LasR+PAI
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0079/annotation300992/range300992/1

https://synbiohub.org/public/igem/BBa_R0079/annotation300992/range300992/1
type: Range
persistent identity: range300992
display id: range300992
version: 1
direction: direction/forward
top level: LasR+PAI
start: 106
end: 125
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation318496
display id: annotation318496
version: 1
top level: LasR+PAI
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0079/annotation318496/range318496/1

https://synbiohub.org/public/igem/BBa_R0079/annotation318496/range318496/1
type: Range
persistent identity: range318496
display id: range318496
version: 1
direction: direction/forward
top level: LasR+PAI
start: 140
end: 145
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation318495
display id: annotation318495
version: 1
top level: LasR+PAI
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0079/annotation318495/range318495/1

https://synbiohub.org/public/igem/BBa_R0079/annotation318495/range318495/1
type: Range
persistent identity: range318495
display id: range318495
version: 1
direction: direction/forward
top level: LasR+PAI
start: 117
end: 122
orientation: inline

BBa_I13406
type: ComponentDefinition
persistent identity: BBa I13406
display id: BBa_I13406
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Pbad/AraC with extra REN sites
creator: jkm
created: 2005-05-03T11:00:00Z
modified: 2015-08-31T04:07:33Z
discontinued: false
dominant: false
experience: experience/None
group u list: _6_
m user id: 0
owner id: 253
owning group id: 6
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: I0500 with extra trailing restriction sites: Eco-Xba-I0500-HindIII-NcoI-Spe-Pst
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I13406_sequence/1

BBa_K137085
type: ComponentDefinition
persistent identity: BBa K137085
display id: BBa_K137085
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: optimized (TA) repeat constitutive promoter with 13 bp between -10 and -35 elements
creator: Allen Lin
created: 2008-08-20T11:00:00Z
modified: 2015-05-08T01:10:10Z
discontinued: false
dominant: false
experience: experience/None
group u list: _187_
m user id: 0
owner id: 3112
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: optimized (TA) repeat constitutive promoter with 13 bp between -10 and -35 elements
mutable notes: None
mutable provenance: PCR
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K137085_sequence/1

BBa_K121011
type: ComponentDefinition
persistent identity: BBa K121011
display id: BBa_K121011
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: promoter (lacI regulated)
creator: Masashi Kajita
created: 2008-10-15T11:00:00Z
modified: 2015-05-08T01:09:41Z
discontinued: false
dominant: false
group u list: _220_
m user id: 0
owner id: 3180
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: pressure inducible lac promoter
mutable notes: na
mutable provenance: na
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -10
sequence annotation: K121011
sequence annotation: CAP binding site
sequence annotation: LacI binding site
sequence annotation: -35
sequence: https://synbiohub.org/public/igem/BBa_K121011_sequence/1

-10
type: SequenceAnnotation
persistent identity: annotation1981861
display id: annotation1981861
version: 1
top level: BBa_K121011
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K121011/annotation1981861/range1981861/1

https://synbiohub.org/public/igem/BBa_K121011/annotation1981861/range1981861/1
type: Range
persistent identity: range1981861
display id: range1981861
version: 1
direction: direction/forward
top level: BBa_K121011
start: 165
end: 168
orientation: inline

K121011
type: SequenceAnnotation
persistent identity: annotation1981858
display id: annotation1981858
version: 1
top level: BBa_K121011
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K121011/annotation1981858/range1981858/1

https://synbiohub.org/public/igem/BBa_K121011/annotation1981858/range1981858/1
type: Range
persistent identity: range1981858
display id: range1981858
version: 1
direction: direction/forward
top level: BBa_K121011
start: 1
end: 232
orientation: inline

CAP binding site
type: SequenceAnnotation
persistent identity: annotation1981859
display id: annotation1981859
version: 1
top level: BBa_K121011
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K121011/annotation1981859/range1981859/1

https://synbiohub.org/public/igem/BBa_K121011/annotation1981859/range1981859/1
type: Range
persistent identity: range1981859
display id: range1981859
version: 1
direction: direction/forward
top level: BBa_K121011
start: 94
end: 131
orientation: inline

LacI binding site
type: SequenceAnnotation
persistent identity: annotation1981862
display id: annotation1981862
version: 1
top level: BBa_K121011
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K121011/annotation1981862/range1981862/1

https://synbiohub.org/public/igem/BBa_K121011/annotation1981862/range1981862/1
type: Range
persistent identity: range1981862
display id: range1981862
version: 1
direction: direction/forward
top level: BBa_K121011
start: 169
end: 203
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1981860
display id: annotation1981860
version: 1
top level: BBa_K121011
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K121011/annotation1981860/range1981860/1

https://synbiohub.org/public/igem/BBa_K121011/annotation1981860/range1981860/1
type: Range
persistent identity: range1981860
display id: range1981860
version: 1
direction: direction/forward
top level: BBa_K121011
start: 141
end: 146
orientation: inline

BBa_I746361
type: ComponentDefinition
persistent identity: BBa I746361
display id: BBa_I746361
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PO promoter from P2 phage
creator: Stefan Milde
created: 2007-09-10T11:00:00Z
modified: 2015-08-31T04:08:04Z
discontinued: false
dominant: false
group u list: _116_
m user id: 0
owner id: 2122
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This is the PO promoter taken from the P2 phage genome. It is an inducible promoter that is activated by a class of activators, including P2 ogr (I746350), PSP3 pag (I746351) and phiR73 delta (I746352). These different activators should cause different levels of activity of the PO promoter.
mutable notes: no special considerations
mutable provenance: The source of the DNA is the P2 phage genome.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: PO
sequence: https://synbiohub.org/public/igem/BBa_I746361_sequence/1

PO
type: SequenceAnnotation
persistent identity: annotation1943784
display id: annotation1943784
version: 1
top level: BBa_I746361
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I746361/annotation1943784/range1943784/1

https://synbiohub.org/public/igem/BBa_I746361/annotation1943784/range1943784/1
type: Range
persistent identity: range1943784
display id: range1943784
version: 1
direction: direction/forward
top level: BBa_I746361
start: 1
end: 92
orientation: inline

BBa_K113009
type: ComponentDefinition
persistent identity: BBa K113009
display id: BBa_K113009
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pBad/araC
creator: Zheng Wei, Wang Jinyu
created: 2008-10-25T11:00:00Z
modified: 2015-05-08T01:09:20Z
discontinued: false
dominant: false
experience: experience/None
group u list: _254_
m user id: 0
owner id: 2711
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: It is the same as BBa_I0050. But the part seems not working so we make a new one, which the template is from iHKU team.
mutable notes: later
mutable provenance: iHKU team
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K113009_sequence/1

RhI
type: ComponentDefinition
persistent identity: BBa I14017
display id: BBa_I14017
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: P(Rhl)
creator: Vikram Vijayan, Allen Hsu, Lawrence Fomundam
created: 2004-08-01T11:00:00Z
modified: 2015-08-31T04:07:37Z
discontinued: false
dominant: true
experience: experience/None
group u list: _4_
m user id: 0
owner id: 171
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: RhlR and C4HSL regulated promoter.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: modified P(Rhl)
sequence annotation: -35
sequence annotation: RhlR
sequence: https://synbiohub.org/public/igem/BBa_I14017_sequence/1

modified P(Rhl)
type: SequenceAnnotation
persistent identity: annotation1043006
display id: annotation1043006
version: 1
top level: RhI
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_I14017/annotation1043006/range1043006/1

https://synbiohub.org/public/igem/BBa_I14017/annotation1043006/range1043006/1
type: Range
persistent identity: range1043006
display id: range1043006
version: 1
direction: direction/forward
top level: RhI
start: 1
end: 51
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1043007
display id: annotation1043007
version: 1
top level: RhI
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I14017/annotation1043007/range1043007/1

https://synbiohub.org/public/igem/BBa_I14017/annotation1043007/range1043007/1
type: Range
persistent identity: range1043007
display id: range1043007
version: 1
direction: direction/forward
top level: RhI
start: 21
end: 50
orientation: inline

RhlR
type: SequenceAnnotation
persistent identity: annotation1043005
display id: annotation1043005
version: 1
top level: RhI
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I14017/annotation1043005/range1043005/1

https://synbiohub.org/public/igem/BBa_I14017/annotation1043005/range1043005/1
type: Range
persistent identity: range1043005
display id: range1043005
version: 1
direction: direction/forward
top level: RhI
start: 1
end: 20
orientation: inline

BBa_K864400
type: ComponentDefinition
persistent identity: BBa K864400
display id: BBa_K864400
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ptac, trp & lac regulated promoter
creator: Erik Lundin
created: 2012-09-24T11:00:00Z
modified: 2015-05-08T01:13:37Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1124_
m user id: 0
owner id: 9827
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: The Ptac promoter is a functional hybrid promoter, derived from the trp and lac promoters, that are regulated by trp and lac [1]. This part also exist together with lacI, part BBa_K180000 [1] Proc. Natl. Acad. Sci. USA, Vol. 80, pp. 21-25
mutable notes: Oligos ordered from SigmaAldrich were annealed to form a double stranded DNA segment, ready to be ligated into any BioBrick backbone.
mutable provenance: Created from oligos ordered from SigmaAldrich. Oligo sequences: Ptac+ AATTCGCGGCCGCTTCTAGAGGAGCTGTTGACAATTAATCATCGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTA Ptac- CTAGTAAATTGTTATCCGCTCACAATTCCACACATTATACGAGCCGATGATTAATTGTCAACAGCTCCTCTAGAAGCGGCCGCG
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: lacO1 site
sequence annotation: -10
sequence annotation: -35
sequence annotation: Ptac
sequence: https://synbiohub.org/public/igem/BBa_K864400_sequence/1

lacO1 site
type: SequenceAnnotation
persistent identity: annotation2197240
display id: annotation2197240
version: 1
top level: BBa_K864400
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K864400/annotation2197240/range2197240/1

https://synbiohub.org/public/igem/BBa_K864400/annotation2197240/range2197240/1
type: Range
persistent identity: range2197240
display id: range2197240
version: 1
direction: direction/forward
top level: BBa_K864400
start: 36
end: 61
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2197238
display id: annotation2197238
version: 1
top level: BBa_K864400
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K864400/annotation2197238/range2197238/1

https://synbiohub.org/public/igem/BBa_K864400/annotation2197238/range2197238/1
type: Range
persistent identity: range2197238
display id: range2197238
version: 1
direction: direction/forward
top level: BBa_K864400
start: 29
end: 33
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2197237
display id: annotation2197237
version: 1
top level: BBa_K864400
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K864400/annotation2197237/range2197237/1

https://synbiohub.org/public/igem/BBa_K864400/annotation2197237/range2197237/1
type: Range
persistent identity: range2197237
display id: range2197237
version: 1
direction: direction/forward
top level: BBa_K864400
start: 7
end: 12
orientation: inline

Ptac
type: SequenceAnnotation
persistent identity: annotation2197236
display id: annotation2197236
version: 1
top level: BBa_K864400
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K864400/annotation2197236/range2197236/1

https://synbiohub.org/public/igem/BBa_K864400/annotation2197236/range2197236/1
type: Range
persistent identity: range2197236
display id: range2197236
version: 1
direction: direction/forward
top level: BBa_K864400
start: 1
end: 40
orientation: inline

BBa_K2168001
type: ComponentDefinition
persistent identity: BBa K2168001
display id: BBa_K2168001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pBAD/araC - (N-6xHis) - Ag43 - sfGFP
creator: Arunavo Chakraborty
created: 2016-10-12T11:00:00Z
modified: 2016-10-13T03:15:53Z
discontinued: false
dominant: false
group u list: _2639_
m user id: 30486
owner id: 30486
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This is a fusion protein with Ag43, a protein from E coli tagged with a 6xHis tag on the N-terminal and sfGFP tagged on C-terminal of the pro-protein. Ag43 causes cell-cell adhesion in E coli, and the 6xHis and sfGFP tags are present to allow convenient study dependence of Ag43 mediated aggregation on Ag43 expression levels, which can be determined from the sfGFP fluoresence and/or quantification of 6xHis tag. The 6xHis tag is included to allow protein concentration estimation in case the sfGFP fails to fold properly.
mutable notes: The location for the 6xHis tag is the same as that for BBa_K2168000(Ag43 tagged with 6xHis on N-terminal under pBAD/araC promoter) and uses the same design principles. For independent folding, a flexible GGGGSGGGGS linker was added between Ag43 and sfGFP, and the linker was choosen to be not very hydrophilic or hydrophobic, since Ag43 lies very close to lipid membranes.
mutable provenance: This part was made from Gibson assemblies from fragments of two previous parts - BBa_K1352000 and BBa_I746908, which code for Ag43 and sfGFP respectively.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: (GGGGS)2 linker
sequence annotation: pBAD
sequence annotation: B0034
sequence annotation: 6xHis
sequence annotation: Ag43(PstI sites removed)
sequence annotation: araC
sequence annotation: sfGFP
sequence annotation: Double terminator (B0015)
sequence: https://synbiohub.org/public/igem/BBa_K2168001_sequence/1

(GGGGS)2 linker
type: SequenceAnnotation
persistent identity: annotation2499487
display id: annotation2499487
version: 1
top level: BBa_K2168001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K2168001/annotation2499487/range2499487/1

https://synbiohub.org/public/igem/BBa_K2168001/annotation2499487/range2499487/1
type: Range
persistent identity: range2499487
display id: range2499487
version: 1
direction: direction/forward
top level: BBa_K2168001
start: 4372
end: 4401
orientation: inline

pBAD
type: SequenceAnnotation
persistent identity: annotation2499484
display id: annotation2499484
version: 1
top level: BBa_K2168001
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K2168001/annotation2499484/range2499484/1

https://synbiohub.org/public/igem/BBa_K2168001/annotation2499484/range2499484/1
type: Range
persistent identity: range2499484
display id: range2499484
version: 1
direction: direction/forward
top level: BBa_K2168001
start: 906
end: 1190
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation2499485
display id: annotation2499485
version: 1
top level: BBa_K2168001
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K2168001/annotation2499485/range2499485/1

https://synbiohub.org/public/igem/BBa_K2168001/annotation2499485/range2499485/1
type: Range
persistent identity: range2499485
display id: range2499485
version: 1
direction: direction/forward
top level: BBa_K2168001
start: 1219
end: 1230
orientation: inline

6xHis
type: SequenceAnnotation
persistent identity: annotation2499490
display id: annotation2499490
version: 1
top level: BBa_K2168001
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K2168001/annotation2499490/range2499490/1

https://synbiohub.org/public/igem/BBa_K2168001/annotation2499490/range2499490/1
type: Range
persistent identity: range2499490
display id: range2499490
version: 1
direction: direction/forward
top level: BBa_K2168001
start: 1396
end: 1413
orientation: inline

Ag43(PstI sites removed)
type: SequenceAnnotation
persistent identity: annotation2499486
display id: annotation2499486
version: 1
top level: BBa_K2168001
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K2168001/annotation2499486/range2499486/1

https://synbiohub.org/public/igem/BBa_K2168001/annotation2499486/range2499486/1
type: Range
persistent identity: range2499486
display id: range2499486
version: 1
direction: direction/forward
top level: BBa_K2168001
start: 1237
end: 4371
orientation: inline

araC
type: SequenceAnnotation
persistent identity: annotation2499465
display id: annotation2499465
version: 1
top level: BBa_K2168001
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K2168001/annotation2499465/range2499465/1

https://synbiohub.org/public/igem/BBa_K2168001/annotation2499465/range2499465/1
type: Range
persistent identity: range2499465
display id: range2499465
version: 1
direction: direction/reverse
top level: BBa_K2168001
start: 1
end: 879
orientation: inline

sfGFP
type: SequenceAnnotation
persistent identity: annotation2499488
display id: annotation2499488
version: 1
top level: BBa_K2168001
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K2168001/annotation2499488/range2499488/1

https://synbiohub.org/public/igem/BBa_K2168001/annotation2499488/range2499488/1
type: Range
persistent identity: range2499488
display id: range2499488
version: 1
direction: direction/forward
top level: BBa_K2168001
start: 4402
end: 5118
orientation: inline

Double terminator (B0015)
type: SequenceAnnotation
persistent identity: annotation2499489
display id: annotation2499489
version: 1
top level: BBa_K2168001
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K2168001/annotation2499489/range2499489/1

https://synbiohub.org/public/igem/BBa_K2168001/annotation2499489/range2499489/1
type: Range
persistent identity: range2499489
display id: range2499489
version: 1
direction: direction/forward
top level: BBa_K2168001
start: 5130
end: 5258
orientation: inline

Zif23
type: ComponentDefinition
persistent identity: BBa R2002
display id: BBa_R2002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter, Zif23 regulated, test: between and after
creator: Ronny Krashinsky
created: 2004-01-29T12:00:00Z
modified: 2015-05-08T01:14:16Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Promoter with standard -10 and -35 sites. A symmetric Zif23 dimer target site is between the -10 and -35 sites, and a symmetric Zif23 dimer target site is after the -10 site.
mutable notes: consensus sequences based on [Jensen98].
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: consensus
sequence annotation: Zif23
sequence annotation: -35
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R2002_sequence/1

consensus
type: SequenceAnnotation
persistent identity: annotation318174
display id: annotation318174
version: 1
top level: Zif23
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_R2002/annotation318174/range318174/1

https://synbiohub.org/public/igem/BBa_R2002/annotation318174/range318174/1
type: Range
persistent identity: range318174
display id: range318174
version: 1
direction: direction/forward
top level: Zif23
start: 1
end: 9
orientation: inline

Zif23
type: SequenceAnnotation
persistent identity: annotation318171
display id: annotation318171
version: 1
top level: Zif23
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R2002/annotation318171/range318171/1

https://synbiohub.org/public/igem/BBa_R2002/annotation318171/range318171/1
type: Range
persistent identity: range318171
display id: range318171
version: 1
direction: direction/forward
top level: Zif23
start: 20
end: 33
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation318160
display id: annotation318160
version: 1
top level: Zif23
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R2002/annotation318160/range318160/1

https://synbiohub.org/public/igem/BBa_R2002/annotation318160/range318160/1
type: Range
persistent identity: range318160
display id: range318160
version: 1
direction: direction/forward
top level: Zif23
start: 10
end: 15
orientation: inline

Zif23
type: SequenceAnnotation
persistent identity: annotation318164
display id: annotation318164
version: 1
top level: Zif23
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R2002/annotation318164/range318164/1

https://synbiohub.org/public/igem/BBa_R2002/annotation318164/range318164/1
type: Range
persistent identity: range318164
display id: range318164
version: 1
direction: direction/forward
top level: Zif23
start: 39
end: 52
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation318163
display id: annotation318163
version: 1
top level: Zif23
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R2002/annotation318163/range318163/1

https://synbiohub.org/public/igem/BBa_R2002/annotation318163/range318163/1
type: Range
persistent identity: range318163
display id: range318163
version: 1
direction: direction/forward
top level: Zif23
start: 33
end: 38
orientation: inline

BBa_J34806
type: ComponentDefinition
persistent identity: BBa J34806
display id: BBa_J34806
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: promoter lac induced
creator: Alexandra Choutko
created: 2006-10-25T11:00:00Z
modified: 2015-08-31T04:08:46Z
discontinued: false
dominant: false
experience: experience/None
group u list: _62_
m user id: 0
owner id: 1085
owning group id: 62
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: to be used with transcription factor BBa_J34802
mutable notes: a
mutable provenance: a
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J34806_sequence/1

Pnot(O33)
type: ComponentDefinition
persistent identity: BBa I732203
display id: BBa_I732203
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: NOT Gate Promoter Family Member (D001O33)
creator: Zhan Jian
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:57Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732203_sequence/1

U097O11+D0
type: ComponentDefinition
persistent identity: BBa I732417
display id: BBa_I732417
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member (U097O11+D050O22)
creator: Zhan Jian
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732417_sequence/1

BBa_K180000
type: ComponentDefinition
persistent identity: BBa K180000
display id: BBa_K180000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Hybrid promoter (trp & lac regulated -- tac pR)
creator: Richard Wilson, Stephanie Chambers
created: 2009-10-11T11:00:00Z
modified: 2015-05-08T01:11:06Z
discontinued: false
dominant: false
experience: experience/None
group u list: _278_
m user id: 0
owner id: 4082
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: The Ptac promoter is a functional hybrid promoter, derived from the trp and lac promoters, that are regulated by trp and lac [1]. The Ptac sequence has been extracted by PCR from the commercially available plasmid pMAL-pIII from New England Biolabs (N8101S), with the addition of the biobrick prefix and suffix sequences. [1] Proc. Natl. Acad. Sci. USA, Vol. 80, pp. 21-25
mutable notes: Sequence includes lacIq and Shine-Dalgarno sequence from pMAL-pIII prior to the Ptac promoter to ensure correct functionality of the promoter.
mutable provenance: pMAL-pIII (New England Biolabs N8101S)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: lacIq
sequence annotation: Ptac
sequence: https://synbiohub.org/public/igem/BBa_K180000_sequence/1

lacIq
type: SequenceAnnotation
persistent identity: annotation2044120
display id: annotation2044120
version: 1
top level: BBa_K180000
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K180000/annotation2044120/range2044120/1

https://synbiohub.org/public/igem/BBa_K180000/annotation2044120/range2044120/1
type: Range
persistent identity: range2044120
display id: range2044120
version: 1
direction: direction/forward
top level: BBa_K180000
start: 1
end: 1082
orientation: inline

Ptac
type: SequenceAnnotation
persistent identity: annotation2044121
display id: annotation2044121
version: 1
top level: BBa_K180000
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K180000/annotation2044121/range2044121/1

https://synbiohub.org/public/igem/BBa_K180000/annotation2044121/range2044121/1
type: Range
persistent identity: range2044121
display id: range2044121
version: 1
direction: direction/forward
top level: BBa_K180000
start: 1325
end: 1352
orientation: inline

BBa_J48104
type: ComponentDefinition
persistent identity: BBa J48104
display id: BBa_J48104
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: NikR promoter, a protein of the ribbon helix-helix family of trancription factors that repress expre
creator: Laina Magaya
created: 2006-10-30T12:00:00Z
modified: 2015-08-31T04:08:51Z
discontinued: false
dominant: false
experience: experience/None
group u list: _73_
m user id: 0
owner id: 969
owning group id: 73
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Nickel responsive transcriptional regulator
mutable notes: Cut with Pst I and Xba I
mutable provenance: Escherichia coli UTI89
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J48104_sequence/1

U085O11+D0
type: ComponentDefinition
persistent identity: BBa I732422
display id: BBa_I732422
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member (U085O11+D038O22)
creator: Zhan Jian
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732422_sequence/1

BBa_K116001
type: ComponentDefinition
persistent identity: BBa K116001
display id: BBa_K116001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: nhaA promoter, that can be regulated by pH and nhaR protein.
creator: Chih-Hsien Yang
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:09:32Z
discontinued: false
dominant: true
group u list: _210_
m user id: 0
owner id: 592
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: It's E.coli K12 sodium/proton anti-transport promoter. It can be a pH sensor. We test pH senses in different pH value and Sodium concentration.
mutable notes: It should contain NhaR binding site.
mutable provenance: E.coli K12 MG1655 genomic DNA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K116001_sequence/1

BBa_M13103
type: ComponentDefinition
persistent identity: BBa M13103
display id: BBa_M13103
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: M13K07 gene III promoter
creator: Natalie Kuldell
created: 2006-12-15T12:00:00Z
modified: 2015-05-08T01:13:55Z
discontinued: false
dominant: true
group u list: _45_
m user id: 0
owner id: 314
owning group id: 1
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This part is from the bacteriophage M13 genome, bp 1500-1547 in M13K07. It directs transcription of M13 gene III (BBa_M13503, BBa_M13003). Its identity as a promoter was described in Gene (1980) 11:129-148 based on -10,-35 homology and fragment of genome able to bind RNAP.
mutable notes: none
mutable provenance: New England Biolabs M13K07. Sequenced in 2002 by L. Panganaban.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_M13103_sequence/1

Pnot(O77)
type: ComponentDefinition
persistent identity: BBa I732207
display id: BBa_I732207
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: NOT Gate Promoter Family Member (D001O77)
creator: Zhan Jian
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:57Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732207_sequence/1

cI HK022
type: ComponentDefinition
persistent identity: BBa R1050
display id: BBa_R1050
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter, Standard (HK022 cI regulated)
creator: Reshma Shetty, Drew Endy
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:15Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: [Note: This is the same part as R0050 except that the -10 and -35 sites and spacing have been changed to comply with BBa_S0001]. The pR regulatory region is a 98 base-pair sequence with standard BioBrick prefix and suffix sections on its ends. It contains two operator sites at which HK022 cI protein can bind cooperatively. The -35 and -10 regions from pR are included along with some flanking sequences. The cutoff for the 5' end of the regulatory region was placed just after the pM -10 region which promotes transcription of a gene upstream (thus the pM -10 region is not included). The cutoff for the 3' end of the regulatory region was placed immediately prior to the RBS.
mutable notes: Derived from STHK022N . Modified to comply with BBa_S0001: TTGACA-17N-GATACT Literature claims that there is no cross-talk between HK022 cI regulatory region, Lambda cI, 434 cI and P22 cI, but this has not been verified experimentally by the synthetic biology group at MIT.
mutable provenance: Bacteriophage HK022.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: OR2
sequence annotation: -10
sequence annotation: OR1
sequence annotation: BBa_R1050
sequence annotation: -35
sequence annotation: putative
sequence: https://synbiohub.org/public/igem/BBa_R1050_sequence/1

OR2
type: SequenceAnnotation
persistent identity: annotation2066
display id: annotation2066
version: 1
top level: cI HK022
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R1050/annotation2066/range2066/1

https://synbiohub.org/public/igem/BBa_R1050/annotation2066/range2066/1
type: Range
persistent identity: range2066
display id: range2066
version: 1
direction: direction/forward
top level: cI HK022
start: 14
end: 28
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2071
display id: annotation2071
version: 1
top level: cI HK022
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R1050/annotation2071/range2071/1

https://synbiohub.org/public/igem/BBa_R1050/annotation2071/range2071/1
type: Range
persistent identity: range2071
display id: range2071
version: 1
direction: direction/forward
top level: cI HK022
start: 31
end: 36
orientation: inline

OR1
type: SequenceAnnotation
persistent identity: annotation2067
display id: annotation2067
version: 1
top level: cI HK022
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R1050/annotation2067/range2067/1

https://synbiohub.org/public/igem/BBa_R1050/annotation2067/range2067/1
type: Range
persistent identity: range2067
display id: range2067
version: 1
direction: direction/forward
top level: cI HK022
start: 38
end: 52
orientation: inline

BBa_R1050
type: SequenceAnnotation
persistent identity: annotation7073
display id: annotation7073
version: 1
top level: cI HK022
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_R1050/annotation7073/range7073/1

https://synbiohub.org/public/igem/BBa_R1050/annotation7073/range7073/1
type: Range
persistent identity: range7073
display id: range7073
version: 1
direction: direction/forward
top level: cI HK022
start: 1
end: 56
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2065
display id: annotation2065
version: 1
top level: cI HK022
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R1050/annotation2065/range2065/1

https://synbiohub.org/public/igem/BBa_R1050/annotation2065/range2065/1
type: Range
persistent identity: range2065
display id: range2065
version: 1
direction: direction/forward
top level: cI HK022
start: 8
end: 14
orientation: inline

putative
type: SequenceAnnotation
persistent identity: annotation2070
display id: annotation2070
version: 1
top level: cI HK022
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_R1050/annotation2070/range2070/1

https://synbiohub.org/public/igem/BBa_R1050/annotation2070/range2070/1
type: Range
persistent identity: range2070
display id: range2070
version: 1
direction: direction/forward
top level: cI HK022
start: 44
end: 44
orientation: inline

BBa_K112401
type: ComponentDefinition
persistent identity: BBa K112401
display id: BBa_K112401
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter for recA gene - SOS and Ultrasound Sensitive
creator: Christina Brown
created: 2008-10-20T11:00:00Z
modified: 2015-05-08T01:09:17Z
discontinued: false
dominant: false
group u list: _224_
m user id: 0
owner id: 3241
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: According to Vollmer, et. al in their 1998 Applied and Enviormental Microbiology paper, "Bacterial Stress Responses to 1-Megahertz Pulsed Ultrasound in the Presence of Microbubble", the promoter for the recA gene, a known SOS response gene, is upregulated 4 fold when cells are exposed to high frequency ultrasound and microbubble cavitation.
mutable notes: We did not see the desired response from ultrasound exposure that we expected from this promoter. However, we did not induce the same acoustic cavitation that Vollmer et. al. used to elicit a response from this promoter.
mutable provenance: Cloned from E. coli MG1655 genome.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K112401_sequence/1

BBa_I746363
type: ComponentDefinition
persistent identity: BBa I746363
display id: BBa_I746363
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PV promoter from P2 phage
creator: Stefan Milde
created: 2007-09-10T11:00:00Z
modified: 2015-08-31T04:08:04Z
discontinued: false
dominant: false
group u list: _116_
m user id: 0
owner id: 2122
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is the PF promoter taken from the P2 phage genome. It is an inducible promoter that is activated by a class of activators, including P2 ogr (I746350), PSP3 pag (I746351) and phiR73 delta (I746352). These different activators should cause different levels of activity of the PF promoter.
mutable notes: no special considerations
mutable provenance: P2 phage genome
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: PV
sequence: https://synbiohub.org/public/igem/BBa_I746363_sequence/1

PV
type: SequenceAnnotation
persistent identity: annotation1943786
display id: annotation1943786
version: 1
top level: BBa_I746363
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I746363/annotation1943786/range1943786/1

https://synbiohub.org/public/igem/BBa_I746363/annotation1943786/range1943786/1
type: Range
persistent identity: range1943786
display id: range1943786
version: 1
direction: direction/forward
top level: BBa_I746363
start: 1
end: 91
orientation: inline

BBa_K112405
type: ComponentDefinition
persistent identity: BBa K112405
display id: BBa_K112405
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter for CadA and CadB genes
creator: Christina Brown
created: 2008-10-20T11:00:00Z
modified: 2015-05-08T01:09:17Z
discontinued: false
dominant: false
group u list: _224_
m user id: 0
owner id: 3241
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Our microarray experiment showed that this gene was responsive to sound and ultrasound. Therefore, we cloned the promoter region located approximately 250 base pairs before the start codon for the cadB gene.
mutable notes: There was a relatively high basal level of expression of this gene that made this part difficult to incorporate into our lysis device design.
mutable provenance: cloned from E. coli MG1655 genome located upstream of CadA and CadB genes
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K112405_sequence/1

U073O11+D0
type: ComponentDefinition
persistent identity: BBa I732403
display id: BBa_I732403
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member (U073O11+D062NUL)
creator: Zhan Jian
created: 2007-09-20T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1572
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Might be later edited.
mutable notes: Might be later edited.
mutable provenance: Might be later edited.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: O1
sequence annotation: +1
sequence annotation: consensus elements
sequence: https://synbiohub.org/public/igem/BBa_I732403_sequence/1

O1
type: SequenceAnnotation
persistent identity: annotation1944543
display id: annotation1944543
version: 1
top level: U073O11+D0
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_I732403/annotation1944543/range1944543/1

https://synbiohub.org/public/igem/BBa_I732403/annotation1944543/range1944543/1
type: Range
persistent identity: range1944543
display id: range1944543
version: 1
direction: direction/forward
top level: U073O11+D0
start: 1
end: 20
orientation: inline

+1
type: SequenceAnnotation
persistent identity: annotation1944544
display id: annotation1944544
version: 1
top level: U073O11+D0
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_I732403/annotation1944544/range1944544/1

https://synbiohub.org/public/igem/BBa_I732403/annotation1944544/range1944544/1
type: Range
persistent identity: range1944544
display id: range1944544
version: 1
direction: direction/forward
top level: U073O11+D0
start: 93
end: 93
orientation: inline

consensus elements
type: SequenceAnnotation
persistent identity: annotation1944542
display id: annotation1944542
version: 1
top level: U073O11+D0
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I732403/annotation1944542/range1944542/1

https://synbiohub.org/public/igem/BBa_I732403/annotation1944542/range1944542/1
type: Range
persistent identity: range1944542
display id: range1944542
version: 1
direction: direction/forward
top level: U073O11+D0
start: 57
end: 93
orientation: inline

BBa_J23113
type: ComponentDefinition
persistent identity: BBa J23113
display id: BBa_J23113
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: constitutive promoter family member
creator: John Anderson
created: 2006-08-16T11:00:00Z
modified: 2015-08-31T04:08:40Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: Later
mutable notes: N/A
mutable provenance: Later
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J23113_sequence/1

BBa_K145152
type: ComponentDefinition
persistent identity: BBa K145152
display id: BBa_K145152
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Hybrid promoter: P22 c2 , LacI NOR gate
creator: Jonas Demeulemeester
created: 2008-08-11T11:00:00Z
modified: 2015-05-08T01:10:29Z
discontinued: false
dominant: false
group u list: _257_
m user id: 0
owner id: 2970
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Hybrid promoter constisting of two LacI O2 sites at the ends of the promoter and two recognition sites (OR2 and OR1) for the P22 c2 repressor. The promoter should function as a NOR gate.
mutable notes: a small fragment of OR3 remains.
mutable provenance: synthetic
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: O2 LacI
sequence annotation: O2 P22 c2
sequence annotation: O1 P22 c2
sequence annotation: -35
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_K145152_sequence/1

O2 LacI
type: SequenceAnnotation
persistent identity: annotation1971580
display id: annotation1971580
version: 1
top level: BBa_K145152
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K145152/annotation1971580/range1971580/1

https://synbiohub.org/public/igem/BBa_K145152/annotation1971580/range1971580/1
type: Range
persistent identity: range1971580
display id: range1971580
version: 1
direction: direction/forward
top level: BBa_K145152
start: 115
end: 135
orientation: inline

O2 P22 c2
type: SequenceAnnotation
persistent identity: annotation1971581
display id: annotation1971581
version: 1
top level: BBa_K145152
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K145152/annotation1971581/range1971581/1

https://synbiohub.org/public/igem/BBa_K145152/annotation1971581/range1971581/1
type: Range
persistent identity: range1971581
display id: range1971581
version: 1
direction: direction/forward
top level: BBa_K145152
start: 59
end: 76
orientation: inline

O1 P22 c2
type: SequenceAnnotation
persistent identity: annotation1971587
display id: annotation1971587
version: 1
top level: BBa_K145152
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K145152/annotation1971587/range1971587/1

https://synbiohub.org/public/igem/BBa_K145152/annotation1971587/range1971587/1
type: Range
persistent identity: range1971587
display id: range1971587
version: 1
direction: direction/forward
top level: BBa_K145152
start: 82
end: 97
orientation: inline

O2 LacI
type: SequenceAnnotation
persistent identity: annotation1971579
display id: annotation1971579
version: 1
top level: BBa_K145152
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K145152/annotation1971579/range1971579/1

https://synbiohub.org/public/igem/BBa_K145152/annotation1971579/range1971579/1
type: Range
persistent identity: range1971579
display id: range1971579
version: 1
direction: direction/forward
top level: BBa_K145152
start: 1
end: 21
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1971557
display id: annotation1971557
version: 1
top level: BBa_K145152
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K145152/annotation1971557/range1971557/1

https://synbiohub.org/public/igem/BBa_K145152/annotation1971557/range1971557/1
type: Range
persistent identity: range1971557
display id: range1971557
version: 1
direction: direction/forward
top level: BBa_K145152
start: 56
end: 61
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1971558
display id: annotation1971558
version: 1
top level: BBa_K145152
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K145152/annotation1971558/range1971558/1

https://synbiohub.org/public/igem/BBa_K145152/annotation1971558/range1971558/1
type: Range
persistent identity: range1971558
display id: range1971558
version: 1
direction: direction/forward
top level: BBa_K145152
start: 78
end: 83
orientation: inline

las TetO
type: ComponentDefinition
persistent identity: BBa I14015
display id: BBa_I14015
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: P(Las) TetO
creator: Vikram Vijayan, Allen Hsu, Lawrence Fomundam
created: 2004-07-29T11:00:00Z
modified: 2015-08-31T04:07:37Z
discontinued: false
dominant: false
experience: experience/None
group u list: _4_
m user id: 0
owner id: 171
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: LasR and 3OC12HSL regulated promoter with a single Tet Operator (binding site for TetR repressor)
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: TetR
sequence annotation: -35
sequence annotation: -10
sequence annotation: R0079
sequence: https://synbiohub.org/public/igem/BBa_I14015_sequence/1

TetR
type: SequenceAnnotation
persistent identity: annotation1017910
display id: annotation1017910
version: 1
top level: las TetO
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I14015/annotation1017910/range1017910/1

https://synbiohub.org/public/igem/BBa_I14015/annotation1017910/range1017910/1
type: Range
persistent identity: range1017910
display id: range1017910
version: 1
direction: direction/forward
top level: las TetO
start: 152
end: 170
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1026120
display id: annotation1026120
version: 1
top level: las TetO
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I14015/annotation1026120/range1026120/1

https://synbiohub.org/public/igem/BBa_I14015/annotation1026120/range1026120/1
type: Range
persistent identity: range1026120
display id: range1026120
version: 1
direction: direction/forward
top level: las TetO
start: 117
end: 122
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1026116
display id: annotation1026116
version: 1
top level: las TetO
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I14015/annotation1026116/range1026116/1

https://synbiohub.org/public/igem/BBa_I14015/annotation1026116/range1026116/1
type: Range
persistent identity: range1026116
display id: range1026116
version: 1
direction: direction/forward
top level: las TetO
start: 140
end: 145
orientation: inline

R0079
type: SequenceAnnotation
persistent identity: annotation1017909
display id: annotation1017909
version: 1
top level: las TetO
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I14015/annotation1017909/range1017909/1

https://synbiohub.org/public/igem/BBa_I14015/annotation1017909/range1017909/1
type: Range
persistent identity: range1017909
display id: range1017909
version: 1
direction: direction/forward
top level: las TetO
start: 1
end: 151
orientation: inline

pBAD
type: ComponentDefinition
persistent identity: BBa K206000
display id: BBa_K206000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pBAD strong
creator: Amelia Hardjasa
created: 2009-10-13T11:00:00Z
modified: 2015-05-08T01:11:23Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _307_
m user id: 0
owner id: 4172
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: pBAD is an E.coli promoter that is induced by L-arabinose. K206000 is a mutagenized pBAD promoter that is responsive to lower concentrations of arabinose than wild type ( I13453 ) and, additionally, exhibits a higher maximum of protein expression as measured by coupling to a fluorescent reporter.
mutable notes: There were no special design considerations.
mutable provenance: The sequence was obtained by applying all mutations that upregulated AraC binding and subsequent promoter activity listed in reference [1].
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: promoter
sequence annotation: AraI2
sequence annotation: AraI1
sequence: https://synbiohub.org/public/igem/BBa_K206000_sequence/1

promoter
type: SequenceAnnotation
persistent identity: annotation2049252
display id: annotation2049252
version: 1
top level: pBAD
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K206000/annotation2049252/range2049252/1

https://synbiohub.org/public/igem/BBa_K206000/annotation2049252/range2049252/1
type: Range
persistent identity: range2049252
display id: range2049252
version: 1
direction: direction/forward
top level: pBAD
start: 1
end: 131
orientation: inline

AraI2
type: SequenceAnnotation
persistent identity: annotation2049254
display id: annotation2049254
version: 1
top level: pBAD
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K206000/annotation2049254/range2049254/1

https://synbiohub.org/public/igem/BBa_K206000/annotation2049254/range2049254/1
type: Range
persistent identity: range2049254
display id: range2049254
version: 1
direction: direction/forward
top level: pBAD
start: 61
end: 78
orientation: inline

AraI1
type: SequenceAnnotation
persistent identity: annotation2049253
display id: annotation2049253
version: 1
top level: pBAD
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K206000/annotation2049253/range2049253/1

https://synbiohub.org/public/igem/BBa_K206000/annotation2049253/range2049253/1
type: Range
persistent identity: range2049253
display id: range2049253
version: 1
direction: direction/forward
top level: pBAD
start: 40
end: 57
orientation: inline

AraC
type: ComponentDefinition
persistent identity: BBa R0080
display id: BBa_R0080
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter (AraC regulated)
creator: Sara Neves (Fighting Darwins)
created: 2004-01-27T12:00:00Z
modified: 2015-05-08T01:14:15Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: AraC operator, truncated to include araO1, araI1, araI2, c-amp1, and c-amp2 sites. This operator should *activate* transcription in the presence of AraC; b/c the operator lacks the araO2 site, there should not be araC-mediated repression.
mutable provenance: GenBank: J01641 (www.ncbi.nlm.nih.gov)
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -35
sequence annotation: ara1 and ara2
sequence annotation: c-amp2
sequence annotation: araO1
sequence annotation: c-amp1
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R0080_sequence/1

-35
type: SequenceAnnotation
persistent identity: annotation308601
display id: annotation308601
version: 1
top level: AraC
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0080/annotation308601/range308601/1

https://synbiohub.org/public/igem/BBa_R0080/annotation308601/range308601/1
type: Range
persistent identity: range308601
display id: range308601
version: 1
direction: direction/forward
top level: AraC
start: 113
end: 118
orientation: inline

ara1 and ara2
type: SequenceAnnotation
persistent identity: annotation301462
display id: annotation301462
version: 1
top level: AraC
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0080/annotation301462/range301462/1

https://synbiohub.org/public/igem/BBa_R0080/annotation301462/range301462/1
type: Range
persistent identity: range301462
display id: range301462
version: 1
direction: direction/forward
top level: AraC
start: 73
end: 101
orientation: inline

c-amp2
type: SequenceAnnotation
persistent identity: annotation301456
display id: annotation301456
version: 1
top level: AraC
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0080/annotation301456/range301456/1

https://synbiohub.org/public/igem/BBa_R0080/annotation301456/range301456/1
type: Range
persistent identity: range301456
display id: range301456
version: 1
direction: direction/forward
top level: AraC
start: 4
end: 29
orientation: inline

araO1
type: SequenceAnnotation
persistent identity: annotation301457
display id: annotation301457
version: 1
top level: AraC
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_R0080/annotation301457/range301457/1

https://synbiohub.org/public/igem/BBa_R0080/annotation301457/range301457/1
type: Range
persistent identity: range301457
display id: range301457
version: 1
direction: direction/forward
top level: AraC
start: 6
end: 44
orientation: inline

c-amp1
type: SequenceAnnotation
persistent identity: annotation301458
display id: annotation301458
version: 1
top level: AraC
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0080/annotation301458/range301458/1

https://synbiohub.org/public/igem/BBa_R0080/annotation301458/range301458/1
type: Range
persistent identity: range301458
display id: range301458
version: 1
direction: direction/forward
top level: AraC
start: 43
end: 72
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation308602
display id: annotation308602
version: 1
top level: AraC
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0080/annotation308602/range308602/1

https://synbiohub.org/public/igem/BBa_R0080/annotation308602/range308602/1
type: Range
persistent identity: range308602
display id: range308602
version: 1
direction: direction/forward
top level: AraC
start: 136
end: 141
orientation: inline

P3
type: ComponentDefinition
persistent identity: BBa K100002
display id: BBa_K100002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Edited Xylose Regulated Bi-Directional Operator 2
creator: Garrett Tobin
created: 2008-08-17T11:00:00Z
modified: 2015-05-08T01:08:41Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _213_
m user id: 0
owner id: 1752
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part contains the operator region controlling transcription for the xyl operon. This regulatory region is dependent upon the binding of CRP-cAMP and XylR bound to xylose for activation. Upon binding, transcription is activated in both directions (xylFGH and xylAB facing). This part has a scrambled CRP binding site and and altered RNA ploymerase binding site that was designed to more resemble the consensus sequence at an intermediate level.
mutable notes: Both directions were kept in the sequence of the part even though it is intended to work in the right facing direction because of possible looping that may occur as a transcriptional control. The RNA polymerase binding site was altered to make binding stronger, attempting to allow activation when XylR-xylose alone is bound (the absence of CRP-cAMP).
mutable provenance: Taken from E. Coli genome bases 3728830-3729092, base pairs changed at RNA polymerase bining sites.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: RNA poly. Binding Site
sequence annotation: Scrambled CRP Binding Site
sequence: https://synbiohub.org/public/igem/BBa_K100002_sequence/1

RNA poly. Binding Site
type: SequenceAnnotation
persistent identity: annotation1972259
display id: annotation1972259
version: 1
top level: P3
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K100002/annotation1972259/range1972259/1

https://synbiohub.org/public/igem/BBa_K100002/annotation1972259/range1972259/1
type: Range
persistent identity: range1972259
display id: range1972259
version: 1
direction: direction/forward
top level: P3
start: 45
end: 50
orientation: inline

RNA poly. Binding Site
type: SequenceAnnotation
persistent identity: annotation1972265
display id: annotation1972265
version: 1
top level: P3
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K100002/annotation1972265/range1972265/1

https://synbiohub.org/public/igem/BBa_K100002/annotation1972265/range1972265/1
type: Range
persistent identity: range1972265
display id: range1972265
version: 1
direction: direction/forward
top level: P3
start: 265
end: 270
orientation: inline

RNA poly. Binding Site
type: SequenceAnnotation
persistent identity: annotation1972264
display id: annotation1972264
version: 1
top level: P3
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K100002/annotation1972264/range1972264/1

https://synbiohub.org/public/igem/BBa_K100002/annotation1972264/range1972264/1
type: Range
persistent identity: range1972264
display id: range1972264
version: 1
direction: direction/forward
top level: P3
start: 243
end: 248
orientation: inline

Scrambled CRP Binding Site
type: SequenceAnnotation
persistent identity: annotation1972262
display id: annotation1972262
version: 1
top level: P3
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K100002/annotation1972262/range1972262/1

https://synbiohub.org/public/igem/BBa_K100002/annotation1972262/range1972262/1
type: Range
persistent identity: range1972262
display id: range1972262
version: 1
direction: direction/forward
top level: P3
start: 88
end: 104
orientation: inline

RNA poly. Binding Site
type: SequenceAnnotation
persistent identity: annotation1972260
display id: annotation1972260
version: 1
top level: P3
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K100002/annotation1972260/range1972260/1

https://synbiohub.org/public/igem/BBa_K100002/annotation1972260/range1972260/1
type: Range
persistent identity: range1972260
display id: range1972260
version: 1
direction: direction/reverse
top level: P3
start: 21
end: 26
orientation: inline

BBa_K180015
type: ComponentDefinition
persistent identity: BBa K180015
display id: BBa_K180015
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Paper - Primary plasmid (part 2) [tac pR, GFP and RhlI generator]
creator: Richard Wilson, Stephanie Chambers
created: 2009-10-18T11:00:00Z
modified: 2015-05-08T01:11:06Z
discontinued: false
dominant: false
experience: experience/None
group u list: _278_
m user id: 0
owner id: 4082
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Part of the iGEM2009 Team:Southampton rock-paper-scissors 'rock' primary plasmid.
mutable notes: Regulated expression of RhlI (C0170) and GFP (E0040) by tac pR (K180000), controlled by presence of lac/IPTG or trp in the cell.
mutable provenance: See relevant basic biobrick parts (K180000, B0034, E0040, B0015).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0034
component: BBa_B0010
component: BBa_B0012
component: BBa_C0170
component: BBa_E0040
component: BBa_K180000
sequence annotation: BBa_B0034
sequence annotation: BBa_E0040
sequence annotation: BBa_C0170
sequence annotation: BBa_K180000
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_K180015_sequence/1

BBa_B0034
type: Component
persistent identity: component2053960
display id: component2053960
version: 1
top level: BBa_K180015
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0034
type: Component
persistent identity: component2053964
display id: component2053964
version: 1
top level: BBa_K180015
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0010
type: Component
persistent identity: component2053967
display id: component2053967
version: 1
top level: BBa_K180015
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
type: Component
persistent identity: component2053969
display id: component2053969
version: 1
top level: BBa_K180015
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_C0170
type: Component
persistent identity: component2053966
display id: component2053966
version: 1
top level: BBa_K180015
access: public
definition: https://synbiohub.org/public/igem/BBa_C0170/1

BBa_E0040
type: Component
persistent identity: component2053962
display id: component2053962
version: 1
top level: BBa_K180015
access: public
definition: https://synbiohub.org/public/igem/BBa_E0040/1

BBa_K180000
type: Component
persistent identity: component2053958
display id: component2053958
version: 1
top level: BBa_K180015
access: public
definition: BBa_K180000

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2053964
display id: annotation2053964
version: 1
top level: BBa_K180015
location: https://synbiohub.org/public/igem/BBa_K180015/annotation2053964/range2053964/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K180015/annotation2053964/range2053964/1
type: Range
persistent identity: range2053964
display id: range2053964
version: 1
direction: direction/reverse
top level: BBa_K180015
start: 2202
end: 2213
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation2053962
display id: annotation2053962
version: 1
top level: BBa_K180015
location: https://synbiohub.org/public/igem/BBa_K180015/annotation2053962/range2053962/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_K180015/annotation2053962/range2053962/1
type: Range
persistent identity: range2053962
display id: range2053962
version: 1
direction: direction/reverse
top level: BBa_K180015
start: 1474
end: 2193
orientation: inline

BBa_C0170
type: SequenceAnnotation
persistent identity: annotation2053966
display id: annotation2053966
version: 1
top level: BBa_K180015
location: https://synbiohub.org/public/igem/BBa_K180015/annotation2053966/range2053966/1
component: BBa_C0170

https://synbiohub.org/public/igem/BBa_K180015/annotation2053966/range2053966/1
type: Range
persistent identity: range2053966
display id: range2053966
version: 1
direction: direction/reverse
top level: BBa_K180015
start: 2220
end: 2828
orientation: inline

BBa_K180000
type: SequenceAnnotation
persistent identity: annotation2053958
display id: annotation2053958
version: 1
top level: BBa_K180015
location: https://synbiohub.org/public/igem/BBa_K180015/annotation2053958/range2053958/1
component: BBa_K180000

https://synbiohub.org/public/igem/BBa_K180015/annotation2053958/range2053958/1
type: Range
persistent identity: range2053958
display id: range2053958
version: 1
direction: direction/reverse
top level: BBa_K180015
start: 1
end: 1447
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2053960
display id: annotation2053960
version: 1
top level: BBa_K180015
location: https://synbiohub.org/public/igem/BBa_K180015/annotation2053960/range2053960/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K180015/annotation2053960/range2053960/1
type: Range
persistent identity: range2053960
display id: range2053960
version: 1
direction: direction/reverse
top level: BBa_K180015
start: 1456
end: 1467
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2053967
display id: annotation2053967
version: 1
top level: BBa_K180015
location: https://synbiohub.org/public/igem/BBa_K180015/annotation2053967/range2053967/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K180015/annotation2053967/range2053967/1
type: Range
persistent identity: range2053967
display id: range2053967
version: 1
direction: direction/forward
top level: BBa_K180015
start: 2837
end: 2916
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2053969
display id: annotation2053969
version: 1
top level: BBa_K180015
location: https://synbiohub.org/public/igem/BBa_K180015/annotation2053969/range2053969/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K180015/annotation2053969/range2053969/1
type: Range
persistent identity: range2053969
display id: range2053969
version: 1
direction: direction/forward
top level: BBa_K180015
start: 2925
end: 2965
orientation: inline

BBa_J64800
type: ComponentDefinition
persistent identity: BBa J64800
display id: BBa_J64800
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RHLR/RHLI Inducible & LasR/LasI repressible Promoter
creator: laura lavery
created: 2007-03-24T12:00:00Z
modified: 2015-05-08T01:08:17Z
discontinued: false
dominant: false
experience: experience/None
group u list: _98_
m user id: 0
owner id: 1431
owning group id: 98
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This part is similar to BBa_R0079 but has an added binding site for RHLR/RHLI in between the -35 and -10 region. Thus this promoter is induced by LasR/LasI but repressed by RHLR/RHLi binding.
mutable notes: There is a 17bp spacer between the -35 and -10 regions and the binding site for RHLR/RHLI is 20bp long. I was able to fit the whole binding site in the spacer region because the last three base pairs of the -35 region and the first three base pairs of the RHLR/RHLI binding site are the same.
mutable provenance: Rust L. et. al., "Analysis of the Pseudomonas aeruginosa Elastase (lasB) Regulatory Region". Journal of Bacteriology, 1996.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: LasR/LasI
sequence annotation: -10
sequence annotation: -35
sequence annotation: RHLR/RHLI
sequence: https://synbiohub.org/public/igem/BBa_J64800_sequence/1

LasR/LasI
type: SequenceAnnotation
persistent identity: annotation1921061
display id: annotation1921061
version: 1
top level: BBa_J64800
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_J64800/annotation1921061/range1921061/1

https://synbiohub.org/public/igem/BBa_J64800/annotation1921061/range1921061/1
type: Range
persistent identity: range1921061
display id: range1921061
version: 1
direction: direction/forward
top level: BBa_J64800
start: 23
end: 40
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1921063
display id: annotation1921063
version: 1
top level: BBa_J64800
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J64800/annotation1921063/range1921063/1

https://synbiohub.org/public/igem/BBa_J64800/annotation1921063/range1921063/1
type: Range
persistent identity: range1921063
display id: range1921063
version: 1
direction: direction/forward
top level: BBa_J64800
start: 42
end: 47
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1921062
display id: annotation1921062
version: 1
top level: BBa_J64800
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J64800/annotation1921062/range1921062/1

https://synbiohub.org/public/igem/BBa_J64800/annotation1921062/range1921062/1
type: Range
persistent identity: range1921062
display id: range1921062
version: 1
direction: direction/forward
top level: BBa_J64800
start: 19
end: 24
orientation: inline

RHLR/RHLI
type: SequenceAnnotation
persistent identity: annotation1921060
display id: annotation1921060
version: 1
top level: BBa_J64800
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_J64800/annotation1921060/range1921060/1

https://synbiohub.org/public/igem/BBa_J64800/annotation1921060/range1921060/1
type: Range
persistent identity: range1921060
display id: range1921060
version: 1
direction: direction/forward
top level: BBa_J64800
start: 1
end: 23
orientation: inline

U085O11+D0
type: ComponentDefinition
persistent identity: BBa I732420
display id: BBa_I732420
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member (U085O11+D014O22)
creator: Zhan Jian
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732420_sequence/1

BBa_K1585116
type: ComponentDefinition
persistent identity: BBa K1585116
display id: BBa_K1585116
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Anderson Promoter with lacI binding site
creator: Tobias Schwanemann
created: 2015-08-31T11:00:00Z
modified: 2015-09-01T02:19:47Z
discontinued: false
dominant: false
experience: experience/Fails
group u list: _2002_
m user id: 24474
owner id: 24474
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Promoters of the Anderson Promoter Collection with lacI binding site derived from [http://parts.igem.org/Part:BBa_R0010 R_0010] to make them [http://openwetware.org/wiki/IPTG IPTG] iducible.
mutable notes: We choosed lacI-binding-site of part BBa_R0010 because we assumed older parts would be more reliable. The part is shipped in backbone [http://parts.igem.org/Part:BBa_J61002 J61002] because the functionality is directly visible by adding IPTG. RFP ([http://parts.igem.org/Part:BBa_E1010 E1010]) should get expressed then.
mutable provenance: Assambly of [http://parts.igem.org/Part:BBa_J23103 J23103] and lacI-binding-site of [http://parts.igem.org/Part:BBa_R0010 R_0010] The "lacI binding site" of Part [http://parts.igem.org/Part:BBa_R0010 R_0010] differs from lacO from Part [http://parts.igem.org/Part:BBa_J33207 BBa_J33207].
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
component: BBa_J23116
component: BBa_K1585999
sequence annotation: BBa_J23116
sequence annotation: BBa_K1585999
sequence: https://synbiohub.org/public/igem/BBa_K1585116_sequence/1

BBa_J23116
type: Component
persistent identity: component2441708
display id: component2441708
version: 1
top level: BBa_K1585116
access: public
definition: BBa_J23116

BBa_K1585999
type: Component
persistent identity: component2441710
display id: component2441710
version: 1
top level: BBa_K1585116
access: public
definition: https://synbiohub.org/public/igem/BBa_K1585999/1

BBa_J23116
type: SequenceAnnotation
persistent identity: annotation2441708
display id: annotation2441708
version: 1
top level: BBa_K1585116
location: https://synbiohub.org/public/igem/BBa_K1585116/annotation2441708/range2441708/1
component: BBa_J23116

https://synbiohub.org/public/igem/BBa_K1585116/annotation2441708/range2441708/1
type: Range
persistent identity: range2441708
display id: range2441708
version: 1
direction: direction/reverse
top level: BBa_K1585116
start: 1
end: 35
orientation: inline

BBa_K1585999
type: SequenceAnnotation
persistent identity: annotation2441710
display id: annotation2441710
version: 1
top level: BBa_K1585116
location: https://synbiohub.org/public/igem/BBa_K1585116/annotation2441710/range2441710/1
component: BBa_K1585999

https://synbiohub.org/public/igem/BBa_K1585116/annotation2441710/range2441710/1
type: Range
persistent identity: range2441710
display id: range2441710
version: 1
direction: direction/reverse
top level: BBa_K1585116
start: 44
end: 78
orientation: inline

Prm +
type: ComponentDefinition
persistent identity: BBa I12006
display id: BBa_I12006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Modified lamdba Prm promoter (repressed by 434 cI)
creator: mcnamara
created: 2004-07-13T11:00:00Z
modified: 2015-08-31T04:07:31Z
discontinued: false
dominant: false
experience: experience/None
group u list: _3_
m user id: 0
owner id: 147
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Lamdba Prm promoter modified to be activated by lamda repressor (cI) and repressed by 434 repressor (cI)
mutable notes: The O-R1 region of 434 contained 14 base pairs as opposed to the 17 base pairs of the O-R3 site of lambda. Also, it was noticed that the O-R3 site of the lambda included part of the -10 site. Hence, to preserve the spacing and the -10 site, the three nucleotides that were in both the -10 site and the lambda O-R3 site were retained. The 14 nucleotides that were in the O-R3 site and not in the -10 site were replaced with the O-R1 site of the 434.
mutable provenance: Bushman(1993), Shih & Gussin (1983)
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: OR2 lambda
sequence annotation: -10
sequence annotation: OR1 434
sequence annotation: OR1 lambda
sequence annotation: -35
sequence: https://synbiohub.org/public/igem/BBa_I12006_sequence/1

OR2 lambda
type: SequenceAnnotation
persistent identity: annotation786518
display id: annotation786518
version: 1
top level: Prm +
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I12006/annotation786518/range786518/1

https://synbiohub.org/public/igem/BBa_I12006/annotation786518/range786518/1
type: Range
persistent identity: range786518
display id: range786518
version: 1
direction: direction/forward
top level: Prm +
start: 33
end: 49
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation837228
display id: annotation837228
version: 1
top level: Prm +
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I12006/annotation837228/range837228/1

https://synbiohub.org/public/igem/BBa_I12006/annotation837228/range837228/1
type: Range
persistent identity: range837228
display id: range837228
version: 1
direction: direction/forward
top level: Prm +
start: 71
end: 76
orientation: inline

OR1 434
type: SequenceAnnotation
persistent identity: annotation786365
display id: annotation786365
version: 1
top level: Prm +
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I12006/annotation786365/range786365/1

https://synbiohub.org/public/igem/BBa_I12006/annotation786365/range786365/1
type: Range
persistent identity: range786365
display id: range786365
version: 1
direction: direction/forward
top level: Prm +
start: 56
end: 69
orientation: inline

OR1 lambda
type: SequenceAnnotation
persistent identity: annotation786500
display id: annotation786500
version: 1
top level: Prm +
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I12006/annotation786500/range786500/1

https://synbiohub.org/public/igem/BBa_I12006/annotation786500/range786500/1
type: Range
persistent identity: range786500
display id: range786500
version: 1
direction: direction/forward
top level: Prm +
start: 9
end: 25
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation837284
display id: annotation837284
version: 1
top level: Prm +
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I12006/annotation837284/range837284/1

https://synbiohub.org/public/igem/BBa_I12006/annotation837284/range837284/1
type: Range
persistent identity: range837284
display id: range837284
version: 1
direction: direction/forward
top level: Prm +
start: 48
end: 53
orientation: inline

BBa_I721001
type: ComponentDefinition
persistent identity: BBa I721001
display id: BBa_I721001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Lead Promoter
creator: Jeffrey Hofmann
created: 2007-10-25T11:00:00Z
modified: 2015-08-31T04:07:53Z
discontinued: false
dominant: false
experience: experience/None
group u list: _113_
m user id: 0
owner id: 1531
owning group id: 58
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: Lead Promoter (Part 4) messed up EcoRI site
mutable notes: none
mutable provenance: ralstonia metallidurans
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I721001_sequence/1

BBa_K180006
type: ComponentDefinition
persistent identity: BBa K180006
display id: BBa_K180006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Game of Life - Primary plasmid (part 2) [lux pR, GFP and LacI generator]
creator: Richard Wilson, Stephanie Chambers
created: 2009-10-15T11:00:00Z
modified: 2015-05-08T01:11:06Z
discontinued: false
dominant: false
experience: experience/None
group u list: _278_
m user id: 0
owner id: 4082
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Part of the iGEM2009 Team:Southampton Game of Life primary plasmid.
mutable notes: Regulated expression of LacI (C0012) and GFP (E0040) by PLUX promoter (R0062), controlled by presence/absence of AHL-LuxR.
mutable provenance: See relevant basic biobrick parts (R0062, C0012, B0034, K180001, B0015)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0034
component: BBa_B0015
component: BBa_R0062
component: BBa_K180001
component: BBa_C0012
sequence annotation: BBa_K180001
sequence annotation: BBa_B0034
sequence annotation: BBa_B0015
sequence annotation: BBa_R0062
sequence annotation: BBa_C0012
sequence: https://synbiohub.org/public/igem/BBa_K180006_sequence/1

BBa_B0034
type: Component
persistent identity: component2267834
display id: component2267834
version: 1
top level: BBa_K180006
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0015
type: Component
persistent identity: component2267845
display id: component2267845
version: 1
top level: BBa_K180006
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_R0062
type: Component
persistent identity: component2267822
display id: component2267822
version: 1
top level: BBa_K180006
access: public
definition: lux pR

BBa_K180001
type: Component
persistent identity: component2267838
display id: component2267838
version: 1
top level: BBa_K180006
access: public
definition: https://synbiohub.org/public/igem/BBa_K180001/1

BBa_B0034
type: Component
persistent identity: component2267828
display id: component2267828
version: 1
top level: BBa_K180006
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_C0012
type: Component
persistent identity: component2267830
display id: component2267830
version: 1
top level: BBa_K180006
access: public
definition: https://synbiohub.org/public/igem/BBa_C0012/1

BBa_K180001
type: SequenceAnnotation
persistent identity: annotation2267838
display id: annotation2267838
version: 1
top level: BBa_K180006
location: https://synbiohub.org/public/igem/BBa_K180006/annotation2267838/range2267838/1
component: BBa_K180001

https://synbiohub.org/public/igem/BBa_K180006/annotation2267838/range2267838/1
type: Range
persistent identity: range2267838
display id: range2267838
version: 1
direction: direction/reverse
top level: BBa_K180006
start: 1261
end: 2014
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2267834
display id: annotation2267834
version: 1
top level: BBa_K180006
location: https://synbiohub.org/public/igem/BBa_K180006/annotation2267834/range2267834/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K180006/annotation2267834/range2267834/1
type: Range
persistent identity: range2267834
display id: range2267834
version: 1
direction: direction/reverse
top level: BBa_K180006
start: 1243
end: 1254
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2267845
display id: annotation2267845
version: 1
top level: BBa_K180006
location: https://synbiohub.org/public/igem/BBa_K180006/annotation2267845/range2267845/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K180006/annotation2267845/range2267845/1
type: Range
persistent identity: range2267845
display id: range2267845
version: 1
direction: direction/reverse
top level: BBa_K180006
start: 2023
end: 2151
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2267828
display id: annotation2267828
version: 1
top level: BBa_K180006
location: https://synbiohub.org/public/igem/BBa_K180006/annotation2267828/range2267828/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K180006/annotation2267828/range2267828/1
type: Range
persistent identity: range2267828
display id: range2267828
version: 1
direction: direction/reverse
top level: BBa_K180006
start: 64
end: 75
orientation: inline

BBa_R0062
type: SequenceAnnotation
persistent identity: annotation2267822
display id: annotation2267822
version: 1
top level: BBa_K180006
location: https://synbiohub.org/public/igem/BBa_K180006/annotation2267822/range2267822/1
component: BBa_R0062

https://synbiohub.org/public/igem/BBa_K180006/annotation2267822/range2267822/1
type: Range
persistent identity: range2267822
display id: range2267822
version: 1
direction: direction/forward
top level: BBa_K180006
start: 1
end: 55
orientation: inline

BBa_C0012
type: SequenceAnnotation
persistent identity: annotation2267830
display id: annotation2267830
version: 1
top level: BBa_K180006
location: https://synbiohub.org/public/igem/BBa_K180006/annotation2267830/range2267830/1
component: BBa_C0012

https://synbiohub.org/public/igem/BBa_K180006/annotation2267830/range2267830/1
type: Range
persistent identity: range2267830
display id: range2267830
version: 1
direction: direction/forward
top level: BBa_K180006
start: 82
end: 1209
orientation: inline

lac Or2-62
type: ComponentDefinition
persistent identity: BBa I12210
display id: BBa_I12210
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: plac Or2-62 (positive)
creator: rwald
created: 2004-08-06T11:00:00Z
modified: 2015-08-31T04:07:31Z
discontinued: false
dominant: false
experience: experience/None
group u list: _3_
m user id: 0
owner id: 120
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable description: This is a modification of the standard plac operon with everything before the -35 site removed, the Or2 site from the lambda promoter inserted at the beginning, and a 17-bp spacer added between the two.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -10
sequence annotation: -35
sequence annotation: cI OR2 (lambda)
sequence annotation: spacer
sequence: https://synbiohub.org/public/igem/BBa_I12210_sequence/1

-10
type: SequenceAnnotation
persistent identity: annotation1031726
display id: annotation1031726
version: 1
top level: lac Or2-62
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I12210/annotation1031726/range1031726/1

https://synbiohub.org/public/igem/BBa_I12210/annotation1031726/range1031726/1
type: Range
persistent identity: range1031726
display id: range1031726
version: 1
direction: direction/forward
top level: lac Or2-62
start: 59
end: 65
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1031725
display id: annotation1031725
version: 1
top level: lac Or2-62
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I12210/annotation1031725/range1031725/1

https://synbiohub.org/public/igem/BBa_I12210/annotation1031725/range1031725/1
type: Range
persistent identity: range1031725
display id: range1031725
version: 1
direction: direction/forward
top level: lac Or2-62
start: 35
end: 40
orientation: inline

cI OR2 (lambda)
type: SequenceAnnotation
persistent identity: annotation1031724
display id: annotation1031724
version: 1
top level: lac Or2-62
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I12210/annotation1031724/range1031724/1

https://synbiohub.org/public/igem/BBa_I12210/annotation1031724/range1031724/1
type: Range
persistent identity: range1031724
display id: range1031724
version: 1
direction: direction/forward
top level: lac Or2-62
start: 1
end: 17
orientation: inline

spacer
type: SequenceAnnotation
persistent identity: annotation1031727
display id: annotation1031727
version: 1
top level: lac Or2-62
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I12210/annotation1031727/range1031727/1

https://synbiohub.org/public/igem/BBa_I12210/annotation1031727/range1031727/1
type: Range
persistent identity: range1031727
display id: range1031727
version: 1
direction: direction/forward
top level: lac Or2-62
start: 18
end: 34
orientation: inline

BBa_K256002
type: ComponentDefinition
persistent identity: BBa K256002
display id: BBa_K256002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: J23101:GFP
creator: iGEM09_NTU-Singapore
created: 2009-10-15T11:00:00Z
modified: 2015-05-08T01:11:40Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _359_
m user id: 0
owner id: 4864
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: GFP Reporter
mutable notes: --
mutable provenance: --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_B0010
component: BBa_E0040
component: BBa_J23101
component: BBa_B0034
component: BBa_B0012
sequence annotation: BBa_B0012
sequence annotation: BBa_B0034
sequence annotation: BBa_B0010
sequence annotation: BBa_E0040
sequence annotation: BBa_J23101
sequence: https://synbiohub.org/public/igem/BBa_K256002_sequence/1

BBa_B0010
type: Component
persistent identity: component2056720
display id: component2056720
version: 1
top level: BBa_K256002
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_E0040
type: Component
persistent identity: component2056719
display id: component2056719
version: 1
top level: BBa_K256002
access: public
definition: https://synbiohub.org/public/igem/BBa_E0040/1

BBa_J23101
type: Component
persistent identity: component2056715
display id: component2056715
version: 1
top level: BBa_K256002
access: public
definition: BBa_J23101

BBa_B0034
type: Component
persistent identity: component2056717
display id: component2056717
version: 1
top level: BBa_K256002
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0012
type: Component
persistent identity: component2056722
display id: component2056722
version: 1
top level: BBa_K256002
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2056722
display id: annotation2056722
version: 1
top level: BBa_K256002
location: https://synbiohub.org/public/igem/BBa_K256002/annotation2056722/range2056722/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K256002/annotation2056722/range2056722/1
type: Range
persistent identity: range2056722
display id: range2056722
version: 1
direction: direction/forward
top level: BBa_K256002
start: 878
end: 918
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2056717
display id: annotation2056717
version: 1
top level: BBa_K256002
location: https://synbiohub.org/public/igem/BBa_K256002/annotation2056717/range2056717/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K256002/annotation2056717/range2056717/1
type: Range
persistent identity: range2056717
display id: range2056717
version: 1
direction: direction/reverse
top level: BBa_K256002
start: 44
end: 55
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2056720
display id: annotation2056720
version: 1
top level: BBa_K256002
location: https://synbiohub.org/public/igem/BBa_K256002/annotation2056720/range2056720/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K256002/annotation2056720/range2056720/1
type: Range
persistent identity: range2056720
display id: range2056720
version: 1
direction: direction/forward
top level: BBa_K256002
start: 790
end: 869
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation2056719
display id: annotation2056719
version: 1
top level: BBa_K256002
location: https://synbiohub.org/public/igem/BBa_K256002/annotation2056719/range2056719/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_K256002/annotation2056719/range2056719/1
type: Range
persistent identity: range2056719
display id: range2056719
version: 1
direction: direction/reverse
top level: BBa_K256002
start: 62
end: 781
orientation: inline

BBa_J23101
type: SequenceAnnotation
persistent identity: annotation2056715
display id: annotation2056715
version: 1
top level: BBa_K256002
location: https://synbiohub.org/public/igem/BBa_K256002/annotation2056715/range2056715/1
component: BBa_J23101

https://synbiohub.org/public/igem/BBa_K256002/annotation2056715/range2056715/1
type: Range
persistent identity: range2056715
display id: range2056715
version: 1
direction: direction/reverse
top level: BBa_K256002
start: 1
end: 35
orientation: inline

BBa_I732451
type: ComponentDefinition
persistent identity: BBa I732451
display id: BBa_I732451
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member (U073O27+D062NUL)
creator: Zhan Jian
created: 2007-10-16T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732451_sequence/1

BBa_K259013
type: ComponentDefinition
persistent identity: BBa K259013
display id: BBa_K259013
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: AraC-RBS-GFP-Terminator
creator: Petros Mina
created: 2009-10-14T11:00:00Z
modified: 2015-05-08T01:11:42Z
discontinued: false
dominant: false
group u list: _357_
m user id: 0
owner id: 5318
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: A part used to quantify the AraC-RBS construct K259007 created by the BCCS-Bristol iGEM'09 team.
mutable notes: RFC10 assembly
mutable provenance: This part was designed with the specified biobricks from the parts registry. AraC occurs naturally in E.coli. GFP occurs in jellyfish species, RBS designed by the Anderson family and Terminator by Resma Shetty.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_J61100
component: BBa_R0080
component: BBa_B0012
component: BBa_B0011
component: BBa_R0081
component: BBa_E0040
sequence annotation: BBa_B0011
sequence annotation: BBa_J61100
sequence annotation: BBa_R0081
sequence annotation: BBa_B0012
sequence annotation: BBa_R0080
sequence annotation: BBa_E0040
sequence: https://synbiohub.org/public/igem/BBa_K259013_sequence/1

BBa_J61100
type: Component
persistent identity: component2041444
display id: component2041444
version: 1
top level: BBa_K259013
access: public
definition: https://synbiohub.org/public/igem/BBa_J61100/1

BBa_R0080
type: Component
persistent identity: component2041443
display id: component2041443
version: 1
top level: BBa_K259013
access: public
definition: AraC

BBa_B0012
type: Component
persistent identity: component2041447
display id: component2041447
version: 1
top level: BBa_K259013
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0011
type: Component
persistent identity: component2041451
display id: component2041451
version: 1
top level: BBa_K259013
access: public
definition: https://synbiohub.org/public/igem/BBa_B0011/1

BBa_R0081
type: Component
persistent identity: component2041436
display id: component2041436
version: 1
top level: BBa_K259013
access: public
definition: https://synbiohub.org/public/igem/BBa_R0081/1

BBa_E0040
type: Component
persistent identity: component2041446
display id: component2041446
version: 1
top level: BBa_K259013
access: public
definition: https://synbiohub.org/public/igem/BBa_E0040/1

BBa_B0011
type: SequenceAnnotation
persistent identity: annotation2041451
display id: annotation2041451
version: 1
top level: BBa_K259013
location: https://synbiohub.org/public/igem/BBa_K259013/annotation2041451/range2041451/1
component: BBa_B0011

https://synbiohub.org/public/igem/BBa_K259013/annotation2041451/range2041451/1
type: Range
persistent identity: range2041451
display id: range2041451
version: 1
direction: direction/forward
top level: BBa_K259013
start: 1144
end: 1189
orientation: inline

BBa_J61100
type: SequenceAnnotation
persistent identity: annotation2041444
display id: annotation2041444
version: 1
top level: BBa_K259013
location: https://synbiohub.org/public/igem/BBa_K259013/annotation2041444/range2041444/1
component: BBa_J61100

https://synbiohub.org/public/igem/BBa_K259013/annotation2041444/range2041444/1
type: Range
persistent identity: range2041444
display id: range2041444
version: 1
direction: direction/reverse
top level: BBa_K259013
start: 349
end: 360
orientation: inline

BBa_R0081
type: SequenceAnnotation
persistent identity: annotation2041436
display id: annotation2041436
version: 1
top level: BBa_K259013
location: https://synbiohub.org/public/igem/BBa_K259013/annotation2041436/range2041436/1
component: BBa_R0081

https://synbiohub.org/public/igem/BBa_K259013/annotation2041436/range2041436/1
type: Range
persistent identity: range2041436
display id: range2041436
version: 1
direction: direction/reverse
top level: BBa_K259013
start: 1
end: 183
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2041447
display id: annotation2041447
version: 1
top level: BBa_K259013
location: https://synbiohub.org/public/igem/BBa_K259013/annotation2041447/range2041447/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K259013/annotation2041447/range2041447/1
type: Range
persistent identity: range2041447
display id: range2041447
version: 1
direction: direction/forward
top level: BBa_K259013
start: 1095
end: 1135
orientation: inline

BBa_R0080
type: SequenceAnnotation
persistent identity: annotation2041443
display id: annotation2041443
version: 1
top level: BBa_K259013
location: https://synbiohub.org/public/igem/BBa_K259013/annotation2041443/range2041443/1
component: BBa_R0080

https://synbiohub.org/public/igem/BBa_K259013/annotation2041443/range2041443/1
type: Range
persistent identity: range2041443
display id: range2041443
version: 1
direction: direction/reverse
top level: BBa_K259013
start: 192
end: 340
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation2041446
display id: annotation2041446
version: 1
top level: BBa_K259013
location: https://synbiohub.org/public/igem/BBa_K259013/annotation2041446/range2041446/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_K259013/annotation2041446/range2041446/1
type: Range
persistent identity: range2041446
display id: range2041446
version: 1
direction: direction/reverse
top level: BBa_K259013
start: 367
end: 1086
orientation: inline

pLacIQ1
type: ComponentDefinition
persistent identity: BBa K091112
display id: BBa_K091112
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pLacIQ1 promoter
creator: Pallavi Penumetcha
created: 2008-06-19T11:00:00Z
modified: 2015-05-08T01:08:37Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _191_
m user id: 0
owner id: 3080
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: this is a modified version of the LacIQ promoter that should bind LacIQ tighter than the original.
mutable notes: no special consideration
mutable provenance: This is a modified version of the LacIQ promoter.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -35
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_K091112_sequence/1

-35
type: SequenceAnnotation
persistent identity: annotation1964077
display id: annotation1964077
version: 1
top level: pLacIQ1
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K091112/annotation1964077/range1964077/1

https://synbiohub.org/public/igem/BBa_K091112/annotation1964077/range1964077/1
type: Range
persistent identity: range1964077
display id: range1964077
version: 1
direction: direction/forward
top level: pLacIQ1
start: 12
end: 17
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1964078
display id: annotation1964078
version: 1
top level: pLacIQ1
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K091112/annotation1964078/range1964078/1

https://synbiohub.org/public/igem/BBa_K091112/annotation1964078/range1964078/1
type: Range
persistent identity: range1964078
display id: range1964078
version: 1
direction: direction/forward
top level: pLacIQ1
start: 37
end: 41
orientation: inline

BBa_I746365
type: ComponentDefinition
persistent identity: BBa I746365
display id: BBa_I746365
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PLL promoter from P4 phage
creator: Stefan Milde
created: 2007-09-10T11:00:00Z
modified: 2015-08-31T04:08:04Z
discontinued: false
dominant: false
group u list: _116_
m user id: 0
owner id: 2122
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This is the PLL promoter taken from the P4 phage genome. It is an inducible promoter that is activated by a class of activators, including P2 ogr (I746350), PSP3 pag (I746351) and phiR73 delta (I746352). These different activators should cause different levels of activity of the PLL promoter.
mutable notes: no special considerations
mutable provenance: P4 phage genome sequence
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: PLL
sequence: https://synbiohub.org/public/igem/BBa_I746365_sequence/1

PLL
type: SequenceAnnotation
persistent identity: annotation1943788
display id: annotation1943788
version: 1
top level: BBa_I746365
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I746365/annotation1943788/range1943788/1

https://synbiohub.org/public/igem/BBa_I746365/annotation1943788/range1943788/1
type: Range
persistent identity: range1943788
display id: range1943788
version: 1
direction: direction/forward
top level: BBa_I746365
start: 1
end: 92
orientation: inline

BBa_K873002
type: ComponentDefinition
persistent identity: BBa K873002
display id: BBa_K873002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: HSP promoter
creator: yue hu
created: 2012-09-17T11:00:00Z
modified: 2016-10-16T09:49:24Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _1134_
m user id: 29300
owner id: 11547
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This part is a heat shock promoter, it refers to the transcription factor sigma32. when the temperature rise, the transcription factor sigma32 expresses a lot and binds to the RNA ploymerase to recognize the conserved sequence in -35 and -10 region, then the promoter starts to transcript.
mutable notes: The whole sequence of this part is only 90bp, so take a futher consideration if you intend to digest it for use.
mutable provenance: We align the HSP promoters from the literature and then find the conserved sequence, then we just modified the promoter of groES of E.coli, and synthesis the part through BIC genescript.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K873002_sequence/1

BBa_J61051
type: ComponentDefinition
persistent identity: BBa J61051
display id: BBa_J61051
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: [Psal1]
creator: John Anderson
created: 2007-02-20T12:00:00Z
modified: 2015-08-31T02:03:00Z
discontinued: false
dominant: false
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Salicylate promoter and nahR
mutable notes: nonh
mutable provenance: non
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J61051_sequence/1

BBa_K137051
type: ComponentDefinition
persistent identity: BBa K137051
display id: BBa_K137051
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: 850 bp inverted tetR promoter
creator: Allen Lin
created: 2008-07-20T11:00:00Z
modified: 2015-05-08T01:10:09Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _187_
m user id: 0
owner id: 3112
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Inverted tetR promoter with noncoding, spacer DNA upstream of it to make the total length 650 bp.
mutable notes: This part is in a series of inverted tetR promoters that have total lengths of 150, 250, 350, 450, 650, and 850 bp. We chose the region upstream of tetR in part Q04400 to be the noncoding DNA segment. This noncoding segment consists of the 3' end of tetR and B0015. None of the parts in this series was long enough to include the start codon of tetR, so a functional tetR protein should not be transcribed.
mutable provenance: Primers were synthesized to bind to part Q04400, and then a PCR reaction was run.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: tetR promoter
sequence: https://synbiohub.org/public/igem/BBa_K137051_sequence/1

tetR promoter
type: SequenceAnnotation
persistent identity: annotation1968024
display id: annotation1968024
version: 1
top level: BBa_K137051
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K137051/annotation1968024/range1968024/1

https://synbiohub.org/public/igem/BBa_K137051/annotation1968024/range1968024/1
type: Range
persistent identity: range1968024
display id: range1968024
version: 1
direction: direction/reverse
top level: BBa_K137051
start: 1
end: 54
orientation: inline

BBa_K101002
type: ComponentDefinition
persistent identity: BBa K101002
display id: BBa_K101002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Dual-Repressed Promoter for p22 cII and TetR
creator: Bennett Swiniarski
created: 2008-07-27T11:00:00Z
modified: 2015-05-08T01:08:41Z
discontinued: false
dominant: false
group u list: _205_
m user id: 0
owner id: 3363
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: k;kljg
mutable notes: TetR usually binds to two operator sites, one located upstream from -35 and one located between -35 and -10. However, p22 mnt also has two operator sites, one between -35 and -10 and another downstream from -10. Since each had an operator site in between -35 and -10, we had to eliminate the TetR operator site in order to allow the promoter to work. TetR was picked because it has a stronger binding than p22 mnt, and so would be more able to use only one site to bind.
mutable provenance: This part was designed by combining sequences from parts R0040 (TetR promoter) and R0053 (p22 cII promoter).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K101002_sequence/1

BBa_K823010
type: ComponentDefinition
persistent identity: BBa K823010
display id: BBa_K823010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Anderson promoter J23113
creator: Korinna Kraft
created: 2012-09-07T11:00:00Z
modified: 2015-07-07T11:47:51Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1081_
m user id: 4206
owner id: 12081
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Anderson promoter J23113 without RFP in backbone vector pSB1C3 to easily fuse the promoter with other reporters e.g. the lux operon.
mutable notes: no considerations
mutable provenance: Partsregistry
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -35 box
sequence annotation: -10 box
sequence annotation: J23113
sequence: https://synbiohub.org/public/igem/BBa_K823010_sequence/1

-35 box
type: SequenceAnnotation
persistent identity: annotation2182535
display id: annotation2182535
version: 1
top level: BBa_K823010
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K823010/annotation2182535/range2182535/1

https://synbiohub.org/public/igem/BBa_K823010/annotation2182535/range2182535/1
type: Range
persistent identity: range2182535
display id: range2182535
version: 1
direction: direction/forward
top level: BBa_K823010
start: 1
end: 6
orientation: inline

-10 box
type: SequenceAnnotation
persistent identity: annotation2182536
display id: annotation2182536
version: 1
top level: BBa_K823010
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K823010/annotation2182536/range2182536/1

https://synbiohub.org/public/igem/BBa_K823010/annotation2182536/range2182536/1
type: Range
persistent identity: range2182536
display id: range2182536
version: 1
direction: direction/forward
top level: BBa_K823010
start: 24
end: 29
orientation: inline

J23113
type: SequenceAnnotation
persistent identity: annotation2182534
display id: annotation2182534
version: 1
top level: BBa_K823010
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K823010/annotation2182534/range2182534/1

https://synbiohub.org/public/igem/BBa_K823010/annotation2182534/range2182534/1
type: Range
persistent identity: range2182534
display id: range2182534
version: 1
direction: direction/forward
top level: BBa_K823010
start: 1
end: 35
orientation: inline

BBa_K137048
type: ComponentDefinition
persistent identity: BBa K137048
display id: BBa_K137048
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: 350 bp inverted tetR promoter
creator: Allen Lin
created: 2008-07-20T11:00:00Z
modified: 2015-05-08T01:10:09Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _187_
m user id: 0
owner id: 3112
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Inverted tetR promoter with noncoding, spacer DNA upstream of it to make the total length 350 bp.
mutable notes: This part is in a series of inverted tetR promoters that have total lengths of 150, 250, 350, 450, 650, and 850 bp. We chose the region upstream of tetR in part Q04400 to be the noncoding DNA segment. This noncoding segment consists of the 3' end of tetR and B0015. None of the parts in this series was long enough to include the start codon of tetR, so a functional tetR protein should not be transcribed.
mutable provenance: Primers were synthesized to bind to part Q04400, and then a PCR reaction was run.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: tetR promoter
sequence: https://synbiohub.org/public/igem/BBa_K137048_sequence/1

tetR promoter
type: SequenceAnnotation
persistent identity: annotation1968021
display id: annotation1968021
version: 1
top level: BBa_K137048
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K137048/annotation1968021/range1968021/1

https://synbiohub.org/public/igem/BBa_K137048/annotation1968021/range1968021/1
type: Range
persistent identity: range1968021
display id: range1968021
version: 1
direction: direction/reverse
top level: BBa_K137048
start: 1
end: 54
orientation: inline

BBa_J64804
type: ComponentDefinition
persistent identity: BBa J64804
display id: BBa_J64804
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: The promoter region (inclusive of regulator binding sites) of the B. subtilis RocDEF operon
creator: Sai Duriseti
created: 2007-03-19T12:00:00Z
modified: 2015-05-08T01:08:17Z
discontinued: false
dominant: false
group u list: _98_
m user id: 0
owner id: 1428
owning group id: 98
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is the reported promoter region from the Bacillus subtilis RocDEF operon as taken from the DBTBS website (http://dbtbs.hgc.jp/). Base 1 to base 21 contains the binding site for the binding factor, RocR. The sequence, TATGCAAAAGAATTTTGCACT, reportedly contains the consensus sequence for binding of this factor. Base 42 to base 62 contains another binding site for the RocR binding factor, with ATATCAGAATGTTTTTGCACC as the binding consensus for this sequence. Bases 113-135 outline the binding site of the binding factor AhrC (CTTGCATTTATATAAAGGGAAAG). Bases 96 to 135 outline the promoter region for this operon (CTTGATTTGGCACAGAACTTGCATTTATATAAAGGGAAAG).
mutable notes: This sequence only outlines the reported promoter region as ascertained from the online resource described above, and may lack nucleotides not described by the operon analysis used.
mutable provenance: Information was obtained from: http://dbtbs.hgc.jp/COG/prom/rocDEF.html.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: RocR Binding Site
sequence annotation: Promoter
sequence annotation: AhrC
sequence: https://synbiohub.org/public/igem/BBa_J64804_sequence/1

RocR Binding Site
type: SequenceAnnotation
persistent identity: annotation1920951
display id: annotation1920951
version: 1
top level: BBa_J64804
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_J64804/annotation1920951/range1920951/1

https://synbiohub.org/public/igem/BBa_J64804/annotation1920951/range1920951/1
type: Range
persistent identity: range1920951
display id: range1920951
version: 1
direction: direction/forward
top level: BBa_J64804
start: 1
end: 21
orientation: inline

RocR Binding Site
type: SequenceAnnotation
persistent identity: annotation1920952
display id: annotation1920952
version: 1
top level: BBa_J64804
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_J64804/annotation1920952/range1920952/1

https://synbiohub.org/public/igem/BBa_J64804/annotation1920952/range1920952/1
type: Range
persistent identity: range1920952
display id: range1920952
version: 1
direction: direction/forward
top level: BBa_J64804
start: 42
end: 62
orientation: inline

Promoter
type: SequenceAnnotation
persistent identity: annotation1920953
display id: annotation1920953
version: 1
top level: BBa_J64804
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J64804/annotation1920953/range1920953/1

https://synbiohub.org/public/igem/BBa_J64804/annotation1920953/range1920953/1
type: Range
persistent identity: range1920953
display id: range1920953
version: 1
direction: direction/forward
top level: BBa_J64804
start: 96
end: 135
orientation: inline

AhrC
type: SequenceAnnotation
persistent identity: annotation1920955
display id: annotation1920955
version: 1
top level: BBa_J64804
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_J64804/annotation1920955/range1920955/1

https://synbiohub.org/public/igem/BBa_J64804/annotation1920955/range1920955/1
type: Range
persistent identity: range1920955
display id: range1920955
version: 1
direction: direction/forward
top level: BBa_J64804
start: 113
end: 131
orientation: inline

BBa_K137031
type: ComponentDefinition
persistent identity: BBa K137031
display id: BBa_K137031
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: constitutive promoter with (C)10 between -10 and -35 elements
creator: Allen Lin
created: 2008-07-20T11:00:00Z
modified: 2015-05-08T01:10:09Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _187_
m user id: 0
owner id: 3112
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: constitutive promoter with (C)10 between -10 and -35 elements
mutable notes: With 10 C repeats between the -10 and -35 elements, the distance between the two elements is optimal for the sigma factor to bind to the promoter. Thus, the promoter should be in the 'on' state.
mutable provenance: This part was PCR amplified from two synthesized primers that bound to each other.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -10
sequence annotation: C repeat
sequence: https://synbiohub.org/public/igem/BBa_K137031_sequence/1

-10
type: SequenceAnnotation
persistent identity: annotation1967768
display id: annotation1967768
version: 1
top level: BBa_K137031
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K137031/annotation1967768/range1967768/1

https://synbiohub.org/public/igem/BBa_K137031/annotation1967768/range1967768/1
type: Range
persistent identity: range1967768
display id: range1967768
version: 1
direction: direction/forward
top level: BBa_K137031
start: 50
end: 55
orientation: inline

C repeat
type: SequenceAnnotation
persistent identity: annotation1967769
display id: annotation1967769
version: 1
top level: BBa_K137031
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K137031/annotation1967769/range1967769/1

https://synbiohub.org/public/igem/BBa_K137031/annotation1967769/range1967769/1
type: Range
persistent identity: range1967769
display id: range1967769
version: 1
direction: direction/forward
top level: BBa_K137031
start: 27
end: 36
orientation: inline

BBa_R2110
type: ComponentDefinition
persistent identity: BBa R2110
display id: BBa_R2110
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter with operator site for C2003
creator: Reshma Shetty
created: 2006-10-20T11:00:00Z
modified: 2015-05-08T01:14:16Z
discontinued: false
dominant: false
group u list: _41_
m user id: 0
owner id: 126
owning group id: 70
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This promoter is designed to bind to C2003.
mutable notes: The placement of the operator site was designed to be near optimal for steric interference between C2003 and RNA polymerase.
mutable provenance: This promoter was designed rationally.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: transcription start site
sequence annotation: C2003 operator site
sequence annotation: -35 site
sequence annotation: -10 site
sequence: https://synbiohub.org/public/igem/BBa_R2110_sequence/1

transcription start site
type: SequenceAnnotation
persistent identity: annotation1903918
display id: annotation1903918
version: 1
top level: BBa_R2110
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_R2110/annotation1903918/range1903918/1

https://synbiohub.org/public/igem/BBa_R2110/annotation1903918/range1903918/1
type: Range
persistent identity: range1903918
display id: range1903918
version: 1
direction: direction/forward
top level: BBa_R2110
start: 53
end: 53
orientation: inline

C2003 operator site
type: SequenceAnnotation
persistent identity: annotation1903919
display id: annotation1903919
version: 1
top level: BBa_R2110
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_R2110/annotation1903919/range1903919/1

https://synbiohub.org/public/igem/BBa_R2110/annotation1903919/range1903919/1
type: Range
persistent identity: range1903919
display id: range1903919
version: 1
direction: direction/forward
top level: BBa_R2110
start: 33
end: 44
orientation: inline

-35 site
type: SequenceAnnotation
persistent identity: annotation1903916
display id: annotation1903916
version: 1
top level: BBa_R2110
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R2110/annotation1903916/range1903916/1

https://synbiohub.org/public/igem/BBa_R2110/annotation1903916/range1903916/1
type: Range
persistent identity: range1903916
display id: range1903916
version: 1
direction: direction/forward
top level: BBa_R2110
start: 17
end: 22
orientation: inline

-10 site
type: SequenceAnnotation
persistent identity: annotation1903917
display id: annotation1903917
version: 1
top level: BBa_R2110
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R2110/annotation1903917/range1903917/1

https://synbiohub.org/public/igem/BBa_R2110/annotation1903917/range1903917/1
type: Range
persistent identity: range1903917
display id: range1903917
version: 1
direction: direction/forward
top level: BBa_R2110
start: 40
end: 45
orientation: inline

Pnot(O21)
type: ComponentDefinition
persistent identity: BBa I732273
display id: BBa_I732273
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member with Hybrid Operator (D001O21)
creator: Zhan Jian
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:57Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732273_sequence/1

BBa_K266001
type: ComponentDefinition
persistent identity: BBa K266001
display id: BBa_K266001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Inverter TetR -> LuxR
creator: UNAM FCIENCIAS-CINVESTAV IRA
created: 2009-10-12T11:00:00Z
modified: 2015-05-08T01:11:43Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _363_
m user id: 0
owner id: 1955
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: BBa_J23100 constitutive promoter directs the expression of tetracycline repressor (TetR). TetR binds to pTet regulatory region resulting in a ''negative control'' of the production of LuxR. The whole system acts as an ''Inverter'' of Tet system controlling LuxR expression. TetR repression is inhibited by the addition of tetracycline or its analog, [http://openwetware.org/wiki/ATc aTc].
mutable notes: None
mutable provenance: The biobricks BBa_K145201 and BBa_K116640 where used for this biobrick
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Inverter
component: BBa_K116640
component: BBa_K145201
sequence annotation: BBa_K145201
sequence annotation: BBa_K116640
sequence: https://synbiohub.org/public/igem/BBa_K266001_sequence/1

BBa_K116640
type: Component
persistent identity: component2287677
display id: component2287677
version: 1
top level: BBa_K266001
access: public
definition: https://synbiohub.org/public/igem/BBa_K116640/1

BBa_K145201
type: Component
persistent identity: component2287653
display id: component2287653
version: 1
top level: BBa_K266001
access: public
definition: https://synbiohub.org/public/igem/BBa_K145201/1

BBa_K145201
type: SequenceAnnotation
persistent identity: annotation2287653
display id: annotation2287653
version: 1
top level: BBa_K266001
location: https://synbiohub.org/public/igem/BBa_K266001/annotation2287653/range2287653/1
component: BBa_K145201

https://synbiohub.org/public/igem/BBa_K266001/annotation2287653/range2287653/1
type: Range
persistent identity: range2287653
display id: range2287653
version: 1
direction: direction/reverse
top level: BBa_K266001
start: 1
end: 883
orientation: inline

BBa_K116640
type: SequenceAnnotation
persistent identity: annotation2287677
display id: annotation2287677
version: 1
top level: BBa_K266001
location: https://synbiohub.org/public/igem/BBa_K266001/annotation2287677/range2287677/1
component: BBa_K116640

https://synbiohub.org/public/igem/BBa_K266001/annotation2287677/range2287677/1
type: Range
persistent identity: range2287677
display id: range2287677
version: 1
direction: direction/reverse
top level: BBa_K266001
start: 892
end: 1890
orientation: inline

BBa_K1585117
type: ComponentDefinition
persistent identity: BBa K1585117
display id: BBa_K1585117
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Anderson Promoter with lacI binding site
creator: Tobias Schwanemann
created: 2015-08-31T11:00:00Z
modified: 2015-09-01T02:23:31Z
discontinued: false
dominant: false
experience: experience/Fails
group u list: _2002_
m user id: 24474
owner id: 24474
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Promoters of the Anderson Promoter Collection with lacI binding site derived from [http://parts.igem.org/Part:BBa_R0010 R_0010] to make them [http://openwetware.org/wiki/IPTG IPTG] iducible.
mutable notes: We choosed lacI-binding-site of part BBa_R0010 because we assumed older parts would be more reliable. The part is shipped in backbone [http://parts.igem.org/Part:BBa_J61002 J61002] because the functionality is directly visible by adding IPTG. RFP ([http://parts.igem.org/Part:BBa_E1010 E1010]) should get expressed then.
mutable provenance: Assambly of [http://parts.igem.org/Part:BBa_J23116 J23116] and lacI-binding-site of [http://parts.igem.org/Part:BBa_R0010 R_0010] The "lacI binding site" of Part [http://parts.igem.org/Part:BBa_R0010 R_0010] differs from lacO from Part [http://parts.igem.org/Part:BBa_J33207 BBa_J33207].
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
component: BBa_J23117
component: BBa_K1585999
sequence annotation: BBa_K1585999
sequence annotation: BBa_J23117
sequence: https://synbiohub.org/public/igem/BBa_K1585117_sequence/1

BBa_J23117
type: Component
persistent identity: component2441711
display id: component2441711
version: 1
top level: BBa_K1585117
access: public
definition: BBa_J23117

BBa_K1585999
type: Component
persistent identity: component2441713
display id: component2441713
version: 1
top level: BBa_K1585117
access: public
definition: https://synbiohub.org/public/igem/BBa_K1585999/1

BBa_K1585999
type: SequenceAnnotation
persistent identity: annotation2441713
display id: annotation2441713
version: 1
top level: BBa_K1585117
location: https://synbiohub.org/public/igem/BBa_K1585117/annotation2441713/range2441713/1
component: BBa_K1585999

https://synbiohub.org/public/igem/BBa_K1585117/annotation2441713/range2441713/1
type: Range
persistent identity: range2441713
display id: range2441713
version: 1
direction: direction/reverse
top level: BBa_K1585117
start: 44
end: 78
orientation: inline

BBa_J23117
type: SequenceAnnotation
persistent identity: annotation2441711
display id: annotation2441711
version: 1
top level: BBa_K1585117
location: https://synbiohub.org/public/igem/BBa_K1585117/annotation2441711/range2441711/1
component: BBa_J23117

https://synbiohub.org/public/igem/BBa_K1585117/annotation2441711/range2441711/1
type: Range
persistent identity: range2441711
display id: range2441711
version: 1
direction: direction/reverse
top level: BBa_K1585117
start: 1
end: 35
orientation: inline

U061O11+D0
type: ComponentDefinition
persistent identity: BBa I732433
display id: BBa_I732433
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member (U061O11+D026O22)
creator: Zhan Jian
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732433_sequence/1

BBa_K136000
type: ComponentDefinition
persistent identity: BBa K136000
display id: BBa_K136000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter of fliL gene from E. coli flagella
creator: Cyprien Maisonnier
created: 2008-08-11T11:00:00Z
modified: 2015-05-08T01:10:02Z
discontinued: true
dominant: false
experience: experience/None
group u list: _211_
m user id: 0
owner id: 3012
owning group id: 9
sample status: Discontinued
status: status/Deleted
bookmark: false
mutable description: ''fliL'' is a class 2 gene that is part of the construction of the flagella ( ''Ordering Genes in a Flagella Pathway by Analysis of Expression Kinetics from Living Bacteria'' - S. Kalir, J. McClure, K. Pabbaraju, C. Southward, M. Ronen, S. Leibler, M. G. Surette, U. Alon, ). Its promoter is consitutively repressed. It is activated by flhD4C2 and by fliA with different forces ( ''Using a Quantitative Blueprint to Reprogram the Dynamics of the Flagella Gene Network'' - Shiraz Kalir and Uri Alon ). A deeper characterization is in project
mutable notes: The primers used to amplify this sequence are : Forward : TCGAATTCGCGGCCGCTTCTAGAGCAAGGGCGTGTAACAGGCAAC Reverse : TCCTGCAGCGGCCGCTACTAGTAGTCATGTGTTGCGGTCTTCCTGTG
mutable provenance: This part comes from the genome of ''E.coli'' K12 strain MG1655.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: Initiation start for transcription 1
sequence annotation: Initiation start for transcription 2
sequence annotation: FlhDC binding site 2
sequence annotation: FlhDC binding site 1
sequence: https://synbiohub.org/public/igem/BBa_K136000_sequence/1

Initiation start for transcription 1
type: SequenceAnnotation
persistent identity: annotation1971566
display id: annotation1971566
version: 1
top level: BBa_K136000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K136000/annotation1971566/range1971566/1

https://synbiohub.org/public/igem/BBa_K136000/annotation1971566/range1971566/1
type: Range
persistent identity: range1971566
display id: range1971566
version: 1
direction: direction/forward
top level: BBa_K136000
start: 116
end: 116
orientation: inline

Initiation start for transcription 2
type: SequenceAnnotation
persistent identity: annotation1971567
display id: annotation1971567
version: 1
top level: BBa_K136000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K136000/annotation1971567/range1971567/1

https://synbiohub.org/public/igem/BBa_K136000/annotation1971567/range1971567/1
type: Range
persistent identity: range1971567
display id: range1971567
version: 1
direction: direction/forward
top level: BBa_K136000
start: 127
end: 127
orientation: inline

FlhDC binding site 2
type: SequenceAnnotation
persistent identity: annotation1971565
display id: annotation1971565
version: 1
top level: BBa_K136000
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K136000/annotation1971565/range1971565/1

https://synbiohub.org/public/igem/BBa_K136000/annotation1971565/range1971565/1
type: Range
persistent identity: range1971565
display id: range1971565
version: 1
direction: direction/forward
top level: BBa_K136000
start: 70
end: 85
orientation: inline

FlhDC binding site 1
type: SequenceAnnotation
persistent identity: annotation1971564
display id: annotation1971564
version: 1
top level: BBa_K136000
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K136000/annotation1971564/range1971564/1

https://synbiohub.org/public/igem/BBa_K136000/annotation1971564/range1971564/1
type: Range
persistent identity: range1971564
display id: range1971564
version: 1
direction: direction/forward
top level: BBa_K136000
start: 44
end: 59
orientation: inline

BBa_K180013
type: ComponentDefinition
persistent identity: BBa K180013
display id: BBa_K180013
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Rock-paper-scissors - Rock secondary plasmid [rhl pR, LacI generator]
creator: Richard Wilson, Stephanie Chambers
created: 2009-10-18T11:00:00Z
modified: 2015-05-08T01:11:06Z
discontinued: false
dominant: false
experience: experience/None
group u list: _278_
m user id: 0
owner id: 4082
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: iGEM2009 Team:Southampton Game of Life secondary plasmid.
mutable notes: Regulated expression of LacI (C0012) by Rhl pR (R0071).
mutable provenance: See relevant basic biobrick parts (R0071, B0034, C0012, B0015)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_R0071
component: BBa_B0034
component: BBa_C0012
component: BBa_B0015
sequence annotation: BBa_B0034
sequence annotation: BBa_C0012
sequence annotation: BBa_R0071
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_K180013_sequence/1

BBa_R0071
type: Component
persistent identity: component2256419
display id: component2256419
version: 1
top level: BBa_K180013
access: public
definition: RhlR+C4

BBa_B0034
type: Component
persistent identity: component2256421
display id: component2256421
version: 1
top level: BBa_K180013
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_C0012
type: Component
persistent identity: component2256423
display id: component2256423
version: 1
top level: BBa_K180013
access: public
definition: https://synbiohub.org/public/igem/BBa_C0012/1

BBa_B0015
type: Component
persistent identity: component2256432
display id: component2256432
version: 1
top level: BBa_K180013
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2256421
display id: annotation2256421
version: 1
top level: BBa_K180013
location: https://synbiohub.org/public/igem/BBa_K180013/annotation2256421/range2256421/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K180013/annotation2256421/range2256421/1
type: Range
persistent identity: range2256421
display id: range2256421
version: 1
direction: direction/reverse
top level: BBa_K180013
start: 62
end: 73
orientation: inline

BBa_C0012
type: SequenceAnnotation
persistent identity: annotation2256423
display id: annotation2256423
version: 1
top level: BBa_K180013
location: https://synbiohub.org/public/igem/BBa_K180013/annotation2256423/range2256423/1
component: BBa_C0012

https://synbiohub.org/public/igem/BBa_K180013/annotation2256423/range2256423/1
type: Range
persistent identity: range2256423
display id: range2256423
version: 1
direction: direction/forward
top level: BBa_K180013
start: 80
end: 1207
orientation: inline

BBa_R0071
type: SequenceAnnotation
persistent identity: annotation2256419
display id: annotation2256419
version: 1
top level: BBa_K180013
location: https://synbiohub.org/public/igem/BBa_K180013/annotation2256419/range2256419/1
component: BBa_R0071

https://synbiohub.org/public/igem/BBa_K180013/annotation2256419/range2256419/1
type: Range
persistent identity: range2256419
display id: range2256419
version: 1
direction: direction/reverse
top level: BBa_K180013
start: 1
end: 53
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2256432
display id: annotation2256432
version: 1
top level: BBa_K180013
location: https://synbiohub.org/public/igem/BBa_K180013/annotation2256432/range2256432/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K180013/annotation2256432/range2256432/1
type: Range
persistent identity: range2256432
display id: range2256432
version: 1
direction: direction/reverse
top level: BBa_K180013
start: 1241
end: 1369
orientation: inline

U097O11+D0
type: ComponentDefinition
persistent identity: BBa I732414
display id: BBa_I732414
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member (U097O11+D014O22)
creator: Zhan Jian
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732414_sequence/1

BBa_I739106
type: ComponentDefinition
persistent identity: BBa I739106
display id: BBa_I739106
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Double Promoter (TetR, negative / P22 cII, negative)
creator: Stefan Luzi
created: 2007-10-15T11:00:00Z
modified: 2015-08-31T04:08:01Z
discontinued: false
dominant: false
group u list: _124_
m user id: 0
owner id: 2183
owning group id: 62
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: asdf
mutable notes: asdf
mutable provenance: Synthetic DNA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: OR1
sequence annotation: R0053 (2x OR1)
sequence annotation: R0040
sequence: https://synbiohub.org/public/igem/BBa_I739106_sequence/1

OR1
type: SequenceAnnotation
persistent identity: annotation1947939
display id: annotation1947939
version: 1
top level: BBa_I739106
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I739106/annotation1947939/range1947939/1

https://synbiohub.org/public/igem/BBa_I739106/annotation1947939/range1947939/1
type: Range
persistent identity: range1947939
display id: range1947939
version: 1
direction: direction/forward
top level: BBa_I739106
start: 49
end: 66
orientation: inline

R0053 (2x OR1)
type: SequenceAnnotation
persistent identity: annotation1947941
display id: annotation1947941
version: 1
top level: BBa_I739106
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_I739106/annotation1947941/range1947941/1

https://synbiohub.org/public/igem/BBa_I739106/annotation1947941/range1947941/1
type: Range
persistent identity: range1947941
display id: range1947941
version: 1
direction: direction/forward
top level: BBa_I739106
start: 49
end: 84
orientation: inline

R0040
type: SequenceAnnotation
persistent identity: annotation1947938
display id: annotation1947938
version: 1
top level: BBa_I739106
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I739106/annotation1947938/range1947938/1

https://synbiohub.org/public/igem/BBa_I739106/annotation1947938/range1947938/1
type: Range
persistent identity: range1947938
display id: range1947938
version: 1
direction: direction/forward
top level: BBa_I739106
start: 1
end: 48
orientation: inline

OR1
type: SequenceAnnotation
persistent identity: annotation1947940
display id: annotation1947940
version: 1
top level: BBa_I739106
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I739106/annotation1947940/range1947940/1

https://synbiohub.org/public/igem/BBa_I739106/annotation1947940/range1947940/1
type: Range
persistent identity: range1947940
display id: range1947940
version: 1
direction: direction/forward
top level: BBa_I739106
start: 67
end: 84
orientation: inline

BBa_I751502
type: ComponentDefinition
persistent identity: BBa I751502
display id: BBa_I751502
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: plux-lac hybrid promoter
creator: Kenichiro Iwasaki
created: 2007-10-24T11:00:00Z
modified: 2015-08-31T04:08:06Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _151_
m user id: 0
owner id: 2188
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: sense TWO INPUTS, activation by AHL and repression by LacI.
mutable notes: Compared with existing BioBrick hybrid promoter R0065, this promoter is different in that its sequence is much shorter with lux box and -35 region overlapping, and that it is repressed by LacI, not cI. Also, the output is clearly detected as shown in Fig. 3.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -35
sequence annotation: O1
sequence annotation: -10
sequence annotation: plac
sequence annotation: luxR binding site
sequence: https://synbiohub.org/public/igem/BBa_I751502_sequence/1

-35
type: SequenceAnnotation
persistent identity: annotation1955560
display id: annotation1955560
version: 1
top level: BBa_I751502
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I751502/annotation1955560/range1955560/1

https://synbiohub.org/public/igem/BBa_I751502/annotation1955560/range1955560/1
type: Range
persistent identity: range1955560
display id: range1955560
version: 1
direction: direction/forward
top level: BBa_I751502
start: 20
end: 26
orientation: inline

O1
type: SequenceAnnotation
persistent identity: annotation1955562
display id: annotation1955562
version: 1
top level: BBa_I751502
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I751502/annotation1955562/range1955562/1

https://synbiohub.org/public/igem/BBa_I751502/annotation1955562/range1955562/1
type: Range
persistent identity: range1955562
display id: range1955562
version: 1
direction: direction/forward
top level: BBa_I751502
start: 27
end: 41
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1955561
display id: annotation1955561
version: 1
top level: BBa_I751502
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I751502/annotation1955561/range1955561/1

https://synbiohub.org/public/igem/BBa_I751502/annotation1955561/range1955561/1
type: Range
persistent identity: range1955561
display id: range1955561
version: 1
direction: direction/forward
top level: BBa_I751502
start: 42
end: 48
orientation: inline

O1
type: SequenceAnnotation
persistent identity: annotation1955563
display id: annotation1955563
version: 1
top level: BBa_I751502
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I751502/annotation1955563/range1955563/1

https://synbiohub.org/public/igem/BBa_I751502/annotation1955563/range1955563/1
type: Range
persistent identity: range1955563
display id: range1955563
version: 1
direction: direction/forward
top level: BBa_I751502
start: 49
end: 74
orientation: inline

plac
type: SequenceAnnotation
persistent identity: annotation1955559
display id: annotation1955559
version: 1
top level: BBa_I751502
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I751502/annotation1955559/range1955559/1

https://synbiohub.org/public/igem/BBa_I751502/annotation1955559/range1955559/1
type: Range
persistent identity: range1955559
display id: range1955559
version: 1
direction: direction/forward
top level: BBa_I751502
start: 20
end: 74
orientation: inline

luxR binding site
type: SequenceAnnotation
persistent identity: annotation1955564
display id: annotation1955564
version: 1
top level: BBa_I751502
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I751502/annotation1955564/range1955564/1

https://synbiohub.org/public/igem/BBa_I751502/annotation1955564/range1955564/1
type: Range
persistent identity: range1955564
display id: range1955564
version: 1
direction: direction/forward
top level: BBa_I751502
start: 1
end: 19
orientation: inline

BBa_K1886017
type: ComponentDefinition
persistent identity: BBa K1886017
display id: BBa_K1886017
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: broken_Ptet
creator: Lejian Jiang
created: 2016-10-11T11:00:00Z
modified: 2016-10-17T11:34:11Z
discontinued: false
dominant: false
group u list: _2351_
m user id: 29387
owner id: 29387
owning group id: 9
status: status/Planning
bookmark: false
mutable description: xxx
mutable notes: xxx
mutable provenance: xxx
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K1886017_sequence/1

BBa_I732450
type: ComponentDefinition
persistent identity: BBa I732450
display id: BBa_I732450
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member (U073O26+D062NUL)
creator: Zhan Jian
created: 2007-10-16T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732450_sequence/1

pMalT
type: ComponentDefinition
persistent identity: BBa J03007
display id: BBa_J03007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Maltose specific promotor
creator: James Godman
created: 2005-11-03T12:00:00Z
modified: 2015-08-31T04:08:13Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _15_
m user id: 0
owner id: 405
owning group id: 15
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Binds Maltose and stimulates transcription
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J03007_sequence/1

BBa_K256018
type: ComponentDefinition
persistent identity: BBa K256018
display id: BBa_K256018
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: J23119:IFP
creator: iGEM09_NTU-Singapore
created: 2009-10-16T11:00:00Z
modified: 2015-05-08T01:11:41Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _359_
m user id: 0
owner id: 4864
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: IFP Generator
mutable notes: --
mutable provenance: --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_J23119
component: BBa_K256008
component: BBa_B0012
component: BBa_B0034
component: BBa_B0010
sequence annotation: BBa_J23119
sequence annotation: BBa_B0012
sequence annotation: BBa_B0010
sequence annotation: BBa_B0034
sequence annotation: BBa_K256008
sequence: https://synbiohub.org/public/igem/BBa_K256018_sequence/1

BBa_J23119
type: Component
persistent identity: component2048508
display id: component2048508
version: 1
top level: BBa_K256018
access: public
definition: BBa_J23119

BBa_K256008
type: Component
persistent identity: component2048511
display id: component2048511
version: 1
top level: BBa_K256018
access: public
definition: https://synbiohub.org/public/igem/BBa_K256008/1

BBa_B0012
type: Component
persistent identity: component2048514
display id: component2048514
version: 1
top level: BBa_K256018
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0034
type: Component
persistent identity: component2048510
display id: component2048510
version: 1
top level: BBa_K256018
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0010
type: Component
persistent identity: component2048512
display id: component2048512
version: 1
top level: BBa_K256018
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_J23119
type: SequenceAnnotation
persistent identity: annotation2048508
display id: annotation2048508
version: 1
top level: BBa_K256018
location: https://synbiohub.org/public/igem/BBa_K256018/annotation2048508/range2048508/1
component: BBa_J23119

https://synbiohub.org/public/igem/BBa_K256018/annotation2048508/range2048508/1
type: Range
persistent identity: range2048508
display id: range2048508
version: 1
direction: direction/reverse
top level: BBa_K256018
start: 1
end: 35
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2048514
display id: annotation2048514
version: 1
top level: BBa_K256018
location: https://synbiohub.org/public/igem/BBa_K256018/annotation2048514/range2048514/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K256018/annotation2048514/range2048514/1
type: Range
persistent identity: range2048514
display id: range2048514
version: 1
direction: direction/forward
top level: BBa_K256018
start: 1127
end: 1167
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2048512
display id: annotation2048512
version: 1
top level: BBa_K256018
location: https://synbiohub.org/public/igem/BBa_K256018/annotation2048512/range2048512/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K256018/annotation2048512/range2048512/1
type: Range
persistent identity: range2048512
display id: range2048512
version: 1
direction: direction/forward
top level: BBa_K256018
start: 1039
end: 1118
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2048510
display id: annotation2048510
version: 1
top level: BBa_K256018
location: https://synbiohub.org/public/igem/BBa_K256018/annotation2048510/range2048510/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K256018/annotation2048510/range2048510/1
type: Range
persistent identity: range2048510
display id: range2048510
version: 1
direction: direction/reverse
top level: BBa_K256018
start: 44
end: 55
orientation: inline

BBa_K256008
type: SequenceAnnotation
persistent identity: annotation2048511
display id: annotation2048511
version: 1
top level: BBa_K256018
location: https://synbiohub.org/public/igem/BBa_K256018/annotation2048511/range2048511/1
component: BBa_K256008

https://synbiohub.org/public/igem/BBa_K256018/annotation2048511/range2048511/1
type: Range
persistent identity: range2048511
display id: range2048511
version: 1
direction: direction/reverse
top level: BBa_K256018
start: 62
end: 1030
orientation: inline

BBa_K137046
type: ComponentDefinition
persistent identity: BBa K137046
display id: BBa_K137046
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: 150 bp inverted tetR promoter
creator: Allen Lin
created: 2008-07-20T11:00:00Z
modified: 2015-05-08T01:10:09Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _187_
m user id: 0
owner id: 3112
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: tetR promoter with noncoding, spacer DNA upstream of it to make the total length 150 bp
mutable notes: This part is in a series of tetR promoter that have total lengths of 150, 250, 350, 450, 650, and 850 bp. We chose the region upstream of tetR in part Q04400 to be the noncoding DNA segment. This noncoding segment consists of the 3' end of tetR and B0015. None of the parts in this series was long enough to include the start codon of tetR, so a functional tetR protein should not be transcribed.
mutable provenance: Primers were synthesized to bind to part Q04400, and then a PCR reaction was run.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: tetR promoter
sequence: https://synbiohub.org/public/igem/BBa_K137046_sequence/1

tetR promoter
type: SequenceAnnotation
persistent identity: annotation1968019
display id: annotation1968019
version: 1
top level: BBa_K137046
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K137046/annotation1968019/range1968019/1

https://synbiohub.org/public/igem/BBa_K137046/annotation1968019/range1968019/1
type: Range
persistent identity: range1968019
display id: range1968019
version: 1
direction: direction/reverse
top level: BBa_K137046
start: 1
end: 54
orientation: inline

Prm +
type: ComponentDefinition
persistent identity: BBa I12007
display id: BBa_I12007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Modified lambda Prm promoter (OR-3 obliterated)
creator: Hans
created: 2004-07-14T11:00:00Z
modified: 2015-08-31T04:07:31Z
discontinued: false
dominant: true
experience: experience/None
group u list: _3_
m user id: 0
owner id: 103
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Lambda Prm promoter modified to be activated but not repressed by the lambda repressor (cI)
mutable notes: In wild type lambda phage, the OR3 site (-27 to -11) reads "tatcccttgcggtgata" on the sense strand of DNA. To prevent lambda repressor (cI) from binding to this site, the 4th through 10th nt of OR3 were replaced by the 7 nt between OR2 and OR1, "aaatagt" (-57 to -51), which in effect mutates 5 nt of the promoter. These nt were chosen to be about halfway between the -10 and -35 boxes of the promoter. Furthermore, since these nt already act as a spacer in wild-type phage, it is hoped they will not create undesired interactions.
mutable provenance: Shih and Gussin (1983)
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: OR2
sequence annotation: -10
sequence annotation: -35
sequence annotation: OR1
sequence annotation: mutate to obliterate OR3
sequence: https://synbiohub.org/public/igem/BBa_I12007_sequence/1

OR2
type: SequenceAnnotation
persistent identity: annotation937702
display id: annotation937702
version: 1
top level: Prm +
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I12007/annotation937702/range937702/1

https://synbiohub.org/public/igem/BBa_I12007/annotation937702/range937702/1
type: Range
persistent identity: range937702
display id: range937702
version: 1
direction: direction/forward
top level: Prm +
start: 33
end: 49
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation937705
display id: annotation937705
version: 1
top level: Prm +
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I12007/annotation937705/range937705/1

https://synbiohub.org/public/igem/BBa_I12007/annotation937705/range937705/1
type: Range
persistent identity: range937705
display id: range937705
version: 1
direction: direction/forward
top level: Prm +
start: 71
end: 76
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation937703
display id: annotation937703
version: 1
top level: Prm +
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I12007/annotation937703/range937703/1

https://synbiohub.org/public/igem/BBa_I12007/annotation937703/range937703/1
type: Range
persistent identity: range937703
display id: range937703
version: 1
direction: direction/forward
top level: Prm +
start: 48
end: 53
orientation: inline

OR1
type: SequenceAnnotation
persistent identity: annotation937701
display id: annotation937701
version: 1
top level: Prm +
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I12007/annotation937701/range937701/1

https://synbiohub.org/public/igem/BBa_I12007/annotation937701/range937701/1
type: Range
persistent identity: range937701
display id: range937701
version: 1
direction: direction/forward
top level: Prm +
start: 9
end: 25
orientation: inline

mutate to obliterate OR3
type: SequenceAnnotation
persistent identity: annotation937704
display id: annotation937704
version: 1
top level: Prm +
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_I12007/annotation937704/range937704/1

https://synbiohub.org/public/igem/BBa_I12007/annotation937704/range937704/1
type: Range
persistent identity: range937704
display id: range937704
version: 1
direction: direction/forward
top level: Prm +
start: 59
end: 65
orientation: inline

Pnot(O55)
type: ComponentDefinition
persistent identity: BBa I732205
display id: BBa_I732205
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: NOT Gate Promoter Family Member (D001O55)
creator: Zhan Jian
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:57Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732205_sequence/1

BBa_K1520515
type: ComponentDefinition
persistent identity: BBa K1520515
display id: BBa_K1520515
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: MC7-rbs-luxI-Ter-Pcons2-rbs-luxR-Ter-Prlux-rbs-OMPA-golB-rbs-luxI-Ter.
creator: yue wu
created: 2014-09-22T11:00:00Z
modified: 2015-05-08T01:10:49Z
discontinued: false
dominant: false
group u list: _1902_
m user id: 0
owner id: 21318
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: MC7-rbs-luxI-Ter-Pcons2-rbs-luxR-Ter-Prlux-rbs-OMPA-golB-rbs-luxI-Ter. It is a test of our positive feedback circuit for microcystin.
mutable notes: None
mutable provenance: MC7, luxI, Pcons2, luxR, Prlux, OMPA-golB, Ter
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1520029
component: BBa_K1520504
component: BBa_K1520001
component: BBa_K1520003
component: BBa_K1520507
component: BBa_R0062
sequence annotation: BBa_K1520003
sequence annotation: BBa_K1520504
sequence annotation: BBa_K1520507
sequence annotation: BBa_K1520029
sequence annotation: BBa_R0062
sequence annotation: BBa_K1520001
sequence: https://synbiohub.org/public/igem/BBa_K1520515_sequence/1

BBa_K1520029
type: Component
persistent identity: component2385669
display id: component2385669
version: 1
top level: BBa_K1520515
access: public
definition: https://synbiohub.org/public/igem/BBa_K1520029/1

BBa_K1520504
type: Component
persistent identity: component2385690
display id: component2385690
version: 1
top level: BBa_K1520515
access: public
definition: https://synbiohub.org/public/igem/BBa_K1520504/1

BBa_K1520504
type: Component
persistent identity: component2385668
display id: component2385668
version: 1
top level: BBa_K1520515
access: public
definition: https://synbiohub.org/public/igem/BBa_K1520504/1

BBa_K1520001
type: Component
persistent identity: component2385665
display id: component2385665
version: 1
top level: BBa_K1520515
access: public
definition: https://synbiohub.org/public/igem/BBa_K1520001/1

BBa_K1520003
type: Component
persistent identity: component2385675
display id: component2385675
version: 1
top level: BBa_K1520515
access: public
definition: https://synbiohub.org/public/igem/BBa_K1520003/1

BBa_K1520029
type: Component
persistent identity: component2385691
display id: component2385691
version: 1
top level: BBa_K1520515
access: public
definition: https://synbiohub.org/public/igem/BBa_K1520029/1

BBa_K1520507
type: Component
persistent identity: component2385687
display id: component2385687
version: 1
top level: BBa_K1520515
access: public
definition: https://synbiohub.org/public/igem/BBa_K1520507/1

BBa_R0062
type: Component
persistent identity: component2385677
display id: component2385677
version: 1
top level: BBa_K1520515
access: public
definition: lux pR

BBa_K1520003
type: SequenceAnnotation
persistent identity: annotation2385675
display id: annotation2385675
version: 1
top level: BBa_K1520515
location: https://synbiohub.org/public/igem/BBa_K1520515/annotation2385675/range2385675/1
component: BBa_K1520003

https://synbiohub.org/public/igem/BBa_K1520515/annotation2385675/range2385675/1
type: Range
persistent identity: range2385675
display id: range2385675
version: 1
direction: direction/reverse
top level: BBa_K1520515
start: 948
end: 1926
orientation: inline

BBa_K1520504
type: SequenceAnnotation
persistent identity: annotation2385690
display id: annotation2385690
version: 1
top level: BBa_K1520515
location: https://synbiohub.org/public/igem/BBa_K1520515/annotation2385690/range2385690/1
component: BBa_K1520504

https://synbiohub.org/public/igem/BBa_K1520515/annotation2385690/range2385690/1
type: Range
persistent identity: range2385690
display id: range2385690
version: 1
direction: direction/reverse
top level: BBa_K1520515
start: 2814
end: 3474
orientation: inline

BBa_K1520504
type: SequenceAnnotation
persistent identity: annotation2385668
display id: annotation2385668
version: 1
top level: BBa_K1520515
location: https://synbiohub.org/public/igem/BBa_K1520515/annotation2385668/range2385668/1
component: BBa_K1520504

https://synbiohub.org/public/igem/BBa_K1520515/annotation2385668/range2385668/1
type: Range
persistent identity: range2385668
display id: range2385668
version: 1
direction: direction/reverse
top level: BBa_K1520515
start: 142
end: 802
orientation: inline

BBa_K1520507
type: SequenceAnnotation
persistent identity: annotation2385687
display id: annotation2385687
version: 1
top level: BBa_K1520515
location: https://synbiohub.org/public/igem/BBa_K1520515/annotation2385687/range2385687/1
component: BBa_K1520507

https://synbiohub.org/public/igem/BBa_K1520515/annotation2385687/range2385687/1
type: Range
persistent identity: range2385687
display id: range2385687
version: 1
direction: direction/reverse
top level: BBa_K1520515
start: 1996
end: 2805
orientation: inline

BBa_K1520029
type: SequenceAnnotation
persistent identity: annotation2385669
display id: annotation2385669
version: 1
top level: BBa_K1520515
location: https://synbiohub.org/public/igem/BBa_K1520515/annotation2385669/range2385669/1
component: BBa_K1520029

https://synbiohub.org/public/igem/BBa_K1520515/annotation2385669/range2385669/1
type: Range
persistent identity: range2385669
display id: range2385669
version: 1
direction: direction/reverse
top level: BBa_K1520515
start: 811
end: 939
orientation: inline

BBa_K1520029
type: SequenceAnnotation
persistent identity: annotation2385691
display id: annotation2385691
version: 1
top level: BBa_K1520515
location: https://synbiohub.org/public/igem/BBa_K1520515/annotation2385691/range2385691/1
component: BBa_K1520029

https://synbiohub.org/public/igem/BBa_K1520515/annotation2385691/range2385691/1
type: Range
persistent identity: range2385691
display id: range2385691
version: 1
direction: direction/reverse
top level: BBa_K1520515
start: 3483
end: 3611
orientation: inline

BBa_R0062
type: SequenceAnnotation
persistent identity: annotation2385677
display id: annotation2385677
version: 1
top level: BBa_K1520515
location: https://synbiohub.org/public/igem/BBa_K1520515/annotation2385677/range2385677/1
component: BBa_R0062

https://synbiohub.org/public/igem/BBa_K1520515/annotation2385677/range2385677/1
type: Range
persistent identity: range2385677
display id: range2385677
version: 1
direction: direction/forward
top level: BBa_K1520515
start: 1935
end: 1989
orientation: inline

BBa_K1520001
type: SequenceAnnotation
persistent identity: annotation2385665
display id: annotation2385665
version: 1
top level: BBa_K1520515
location: https://synbiohub.org/public/igem/BBa_K1520515/annotation2385665/range2385665/1
component: BBa_K1520001

https://synbiohub.org/public/igem/BBa_K1520515/annotation2385665/range2385665/1
type: Range
persistent identity: range2385665
display id: range2385665
version: 1
direction: direction/reverse
top level: BBa_K1520515
start: 1
end: 133
orientation: inline

LacZ promo
type: ComponentDefinition
persistent identity: BBa K119000
display id: BBa_K119000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Constitutive weak promoter of lacZ
creator: Miguel Angel Ramirez-Romero
created: 2008-09-27T11:00:00Z
modified: 2015-05-08T01:09:38Z
discontinued: false
dominant: false
experience: experience/None
group u list: _235_
m user id: 0
owner id: 3054
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: LacZ constitutive promoter, without including operators.
mutable notes: We needed two versions of it.
mutable provenance: Lac operon from E. coli.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: LacZ promoter
sequence: https://synbiohub.org/public/igem/BBa_K119000_sequence/1

LacZ promoter
type: SequenceAnnotation
persistent identity: annotation1976843
display id: annotation1976843
version: 1
top level: LacZ promo
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K119000/annotation1976843/range1976843/1

https://synbiohub.org/public/igem/BBa_K119000/annotation1976843/range1976843/1
type: Range
persistent identity: range1976843
display id: range1976843
version: 1
direction: direction/forward
top level: LacZ promo
start: 1
end: 38
orientation: inline

BBa_J23109
type: ComponentDefinition
persistent identity: BBa J23109
display id: BBa_J23109
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: constitutive promoter family member
creator: John Anderson
created: 2006-08-16T11:00:00Z
modified: 2015-08-31T04:08:40Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: true
mutable description: Later
mutable notes: N/A
mutable provenance: Later
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J23109_sequence/1

LacZ prom
type: ComponentDefinition
persistent identity: BBa K119001
display id: BBa_K119001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Mutated LacZ promoter
creator: Miguel Angel Ram??rez-Romero
created: 2008-09-27T11:00:00Z
modified: 2015-05-08T01:09:38Z
discontinued: false
dominant: false
experience: experience/None
group u list: _235_
m user id: 0
owner id: 3054
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Constitutive weak promoter of lacZ which sequence was changed at position 29 (T->G) in order to make it weaker
mutable notes: We needed two version of it.
mutable provenance: Lac Operon from E. coli.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: s_mutation
sequence annotation: LacZ prom mut
sequence: https://synbiohub.org/public/igem/BBa_K119001_sequence/1

s_mutation
type: SequenceAnnotation
persistent identity: annotation1977487
display id: annotation1977487
version: 1
top level: LacZ prom
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_K119001/annotation1977487/range1977487/1

https://synbiohub.org/public/igem/BBa_K119001/annotation1977487/range1977487/1
type: Range
persistent identity: range1977487
display id: range1977487
version: 1
direction: direction/forward
top level: LacZ prom
start: 29
end: 29
orientation: inline

LacZ prom mut
type: SequenceAnnotation
persistent identity: annotation1976842
display id: annotation1976842
version: 1
top level: LacZ prom
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K119001/annotation1976842/range1976842/1

https://synbiohub.org/public/igem/BBa_K119001/annotation1976842/range1976842/1
type: Range
persistent identity: range1976842
display id: range1976842
version: 1
direction: direction/forward
top level: LacZ prom
start: 1
end: 38
orientation: inline

p(tetR)
type: ComponentDefinition
persistent identity: BBa R0040
display id: BBa_R0040
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TetR repressible promoter
creator: June Rhee, Connie Tao, Ty Thomson, Louis Waldman
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Sequence for pTet inverting regulator driven by the TetR protein.
mutable notes: BBa_R0040 TetR-Regulated Promoter is based on a cI promoter. It has been modified to include two TetR binding sites and the BioBrick standard assembly head and tail restriction sites.
mutable provenance: Lutz, R., Bujard, H., Nucleic Acids Research (1997) 25, 1203-1210.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: TetR 2
sequence annotation: TetR 1
sequence annotation: -35
sequence annotation: BBa_R0040
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R0040_sequence/1

TetR 2
type: SequenceAnnotation
persistent identity: annotation1986786
display id: annotation1986786
version: 1
top level: p(tetR)
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1

https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1
type: Range
persistent identity: range1986786
display id: range1986786
version: 1
direction: direction/forward
top level: p(tetR)
start: 26
end: 44
orientation: inline

TetR 1
type: SequenceAnnotation
persistent identity: annotation1986783
display id: annotation1986783
version: 1
top level: p(tetR)
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1

https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1
type: Range
persistent identity: range1986783
display id: range1986783
version: 1
direction: direction/forward
top level: p(tetR)
start: 1
end: 19
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1986785
display id: annotation1986785
version: 1
top level: p(tetR)
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1

https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1
type: Range
persistent identity: range1986785
display id: range1986785
version: 1
direction: direction/forward
top level: p(tetR)
start: 20
end: 25
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1986784
display id: annotation1986784
version: 1
top level: p(tetR)
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1

https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1
type: Range
persistent identity: range1986784
display id: range1986784
version: 1
direction: direction/forward
top level: p(tetR)
start: 1
end: 54
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1986787
display id: annotation1986787
version: 1
top level: p(tetR)
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1

https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1
type: Range
persistent identity: range1986787
display id: range1986787
version: 1
direction: direction/forward
top level: p(tetR)
start: 43
end: 48
orientation: inline

BBa_K1520005
type: ComponentDefinition
persistent identity: BBa K1520005
display id: BBa_K1520005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Plac-rbs-rfp-Ter
creator: yue wu
created: 2014-09-21T11:00:00Z
modified: 2015-05-08T01:10:48Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1902_
m user id: 0
owner id: 21318
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Plac-rbs-rfp-Ter It produce rfp when there is enough IPTG or less lacI
mutable notes: None
mutable provenance: Plac, rfp, Ter
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_K1520500
component: BBa_K1520029
component: BBa_R0010
sequence annotation: BBa_K1520500
sequence annotation: BBa_K1520029
sequence annotation: BBa_R0010
sequence: https://synbiohub.org/public/igem/BBa_K1520005_sequence/1

BBa_K1520500
type: Component
persistent identity: component2384906
display id: component2384906
version: 1
top level: BBa_K1520005
access: public
definition: https://synbiohub.org/public/igem/BBa_K1520500/1

BBa_K1520029
type: Component
persistent identity: component2384907
display id: component2384907
version: 1
top level: BBa_K1520005
access: public
definition: https://synbiohub.org/public/igem/BBa_K1520029/1

BBa_R0010
type: Component
persistent identity: component2384899
display id: component2384899
version: 1
top level: BBa_K1520005
access: public
definition: LacI

BBa_K1520500
type: SequenceAnnotation
persistent identity: annotation2384906
display id: annotation2384906
version: 1
top level: BBa_K1520005
location: https://synbiohub.org/public/igem/BBa_K1520005/annotation2384906/range2384906/1
component: BBa_K1520500

https://synbiohub.org/public/igem/BBa_K1520005/annotation2384906/range2384906/1
type: Range
persistent identity: range2384906
display id: range2384906
version: 1
direction: direction/reverse
top level: BBa_K1520005
start: 209
end: 940
orientation: inline

BBa_K1520029
type: SequenceAnnotation
persistent identity: annotation2384907
display id: annotation2384907
version: 1
top level: BBa_K1520005
location: https://synbiohub.org/public/igem/BBa_K1520005/annotation2384907/range2384907/1
component: BBa_K1520029

https://synbiohub.org/public/igem/BBa_K1520005/annotation2384907/range2384907/1
type: Range
persistent identity: range2384907
display id: range2384907
version: 1
direction: direction/reverse
top level: BBa_K1520005
start: 949
end: 1077
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation2384899
display id: annotation2384899
version: 1
top level: BBa_K1520005
location: https://synbiohub.org/public/igem/BBa_K1520005/annotation2384899/range2384899/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_K1520005/annotation2384899/range2384899/1
type: Range
persistent identity: range2384899
display id: range2384899
version: 1
direction: direction/forward
top level: BBa_K1520005
start: 1
end: 200
orientation: inline

PN
type: ComponentDefinition
persistent identity: BBa K100000
display id: BBa_K100000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Natural Xylose Regulated Bi-Directional Operator
creator: Garrett Tobin
created: 2008-08-17T11:00:00Z
modified: 2015-05-08T01:08:41Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _213_
m user id: 0
owner id: 1752
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part contains the operator region controlling transcription for the xyl operon. This regulatory region is dependent upon the binding of CRP-cAMP and XylR bound to xylose for activation. Upon binding, transcription is activated in both directions (xylFGH and xylAB facing). This part has an unscrambled CRP binding site and and unaltered promoter region (unlike BBa_K100001 through BBa_K100003).
mutable notes: Both directions were kept in the sequence of the part even though it is intended to work in the right facing direction because of possible looping that may occur as a transcriptional control.
mutable provenance: Taken from E. Coli genome bases 3728830-3729092
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: RNA poly. binding site
sequence annotation: CRP binding site
sequence: https://synbiohub.org/public/igem/BBa_K100000_sequence/1

RNA poly. binding site
type: SequenceAnnotation
persistent identity: annotation1972266
display id: annotation1972266
version: 1
top level: PN
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K100000/annotation1972266/range1972266/1

https://synbiohub.org/public/igem/BBa_K100000/annotation1972266/range1972266/1
type: Range
persistent identity: range1972266
display id: range1972266
version: 1
direction: direction/forward
top level: PN
start: 264
end: 269
orientation: inline

RNA poly. binding site
type: SequenceAnnotation
persistent identity: annotation1972196
display id: annotation1972196
version: 1
top level: PN
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K100000/annotation1972196/range1972196/1

https://synbiohub.org/public/igem/BBa_K100000/annotation1972196/range1972196/1
type: Range
persistent identity: range1972196
display id: range1972196
version: 1
direction: direction/forward
top level: PN
start: 45
end: 50
orientation: inline

RNA poly. binding site
type: SequenceAnnotation
persistent identity: annotation1972173
display id: annotation1972173
version: 1
top level: PN
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K100000/annotation1972173/range1972173/1

https://synbiohub.org/public/igem/BBa_K100000/annotation1972173/range1972173/1
type: Range
persistent identity: range1972173
display id: range1972173
version: 1
direction: direction/forward
top level: PN
start: 21
end: 26
orientation: inline

RNA poly. binding site
type: SequenceAnnotation
persistent identity: annotation1972263
display id: annotation1972263
version: 1
top level: PN
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K100000/annotation1972263/range1972263/1

https://synbiohub.org/public/igem/BBa_K100000/annotation1972263/range1972263/1
type: Range
persistent identity: range1972263
display id: range1972263
version: 1
direction: direction/forward
top level: PN
start: 243
end: 248
orientation: inline

CRP binding site
type: SequenceAnnotation
persistent identity: annotation1972261
display id: annotation1972261
version: 1
top level: PN
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K100000/annotation1972261/range1972261/1

https://synbiohub.org/public/igem/BBa_K100000/annotation1972261/range1972261/1
type: Range
persistent identity: range1972261
display id: range1972261
version: 1
direction: direction/forward
top level: PN
start: 90
end: 107
orientation: inline

BBa_K1520010
type: ComponentDefinition
persistent identity: BBa K1520010
display id: BBa_K1520010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Prlux-rbs-rfp-Ter
creator: yue wu
created: 2014-09-21T11:00:00Z
modified: 2015-05-08T01:10:49Z
discontinued: false
dominant: false
group u list: _1902_
m user id: 0
owner id: 21318
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Prlux-rbs-rfp-Ter. It produces rfp when there is enough luxR and AHL.
mutable notes: None
mutable provenance: Prlux, rfp, Ter
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_K1520029
component: BBa_K1520500
component: BBa_R0062
sequence annotation: BBa_K1520029
sequence annotation: BBa_R0062
sequence annotation: BBa_K1520500
sequence: https://synbiohub.org/public/igem/BBa_K1520010_sequence/1

BBa_K1520029
type: Component
persistent identity: component2384955
display id: component2384955
version: 1
top level: BBa_K1520010
access: public
definition: https://synbiohub.org/public/igem/BBa_K1520029/1

BBa_K1520500
type: Component
persistent identity: component2384954
display id: component2384954
version: 1
top level: BBa_K1520010
access: public
definition: https://synbiohub.org/public/igem/BBa_K1520500/1

BBa_R0062
type: Component
persistent identity: component2384949
display id: component2384949
version: 1
top level: BBa_K1520010
access: public
definition: lux pR

BBa_K1520029
type: SequenceAnnotation
persistent identity: annotation2384955
display id: annotation2384955
version: 1
top level: BBa_K1520010
location: https://synbiohub.org/public/igem/BBa_K1520010/annotation2384955/range2384955/1
component: BBa_K1520029

https://synbiohub.org/public/igem/BBa_K1520010/annotation2384955/range2384955/1
type: Range
persistent identity: range2384955
display id: range2384955
version: 1
direction: direction/reverse
top level: BBa_K1520010
start: 804
end: 932
orientation: inline

BBa_R0062
type: SequenceAnnotation
persistent identity: annotation2384949
display id: annotation2384949
version: 1
top level: BBa_K1520010
location: https://synbiohub.org/public/igem/BBa_K1520010/annotation2384949/range2384949/1
component: BBa_R0062

https://synbiohub.org/public/igem/BBa_K1520010/annotation2384949/range2384949/1
type: Range
persistent identity: range2384949
display id: range2384949
version: 1
direction: direction/forward
top level: BBa_K1520010
start: 1
end: 55
orientation: inline

BBa_K1520500
type: SequenceAnnotation
persistent identity: annotation2384954
display id: annotation2384954
version: 1
top level: BBa_K1520010
location: https://synbiohub.org/public/igem/BBa_K1520010/annotation2384954/range2384954/1
component: BBa_K1520500

https://synbiohub.org/public/igem/BBa_K1520010/annotation2384954/range2384954/1
type: Range
persistent identity: range2384954
display id: range2384954
version: 1
direction: direction/reverse
top level: BBa_K1520010
start: 64
end: 795
orientation: inline

U049O11+D0
type: ComponentDefinition
persistent identity: BBa I732441
display id: BBa_I732441
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member (U049O11+D050O22)
creator: Zhan Jian
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732441_sequence/1

BBa_J23102
type: ComponentDefinition
persistent identity: BBa J23102
display id: BBa_J23102
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: constitutive promoter family member
creator: John Anderson
created: 2006-08-03T11:00:00Z
modified: 2015-08-31T04:08:40Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: replace later
mutable notes: N/A
mutable provenance: isolated from library of promoters
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J23102_sequence/1

OmpR
type: ComponentDefinition
persistent identity: BBa R0082
display id: BBa_R0082
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter (OmpR, positive)
creator: Stephen Lee, Roshan Kumar, Joe Levine, Ziyan Chu (Polkadorks, IAP 2004)
created: 2004-01-27T12:00:00Z
modified: 2015-05-08T01:14:15Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Positively regulated, OmpR-controlled promoter. This promoter is taken from the upstream region of ompC. Phosphorylated OmpR binds to the three operator sites and activates transcription.
mutable notes: The promoter includes the three OmpR binding sites: C1, C2, and C3. The promoter starts at -107 and goes up to the transcriptional start site at +1. An alternate version, BBa_R0083, cuts out the C2 and C3 sites. The efficacy of this promoter versus the truncated ompC upstream region (BBa_R0083) or the ompF upstream region (BBa_R0084) is currently unknown.
mutable provenance: NC_000193 E. coli K12
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -35
sequence annotation: C3 OmpR
sequence annotation: C1 OmpR
sequence annotation: -10
sequence annotation: C2 OmpR
sequence: https://synbiohub.org/public/igem/BBa_R0082_sequence/1

-35
type: SequenceAnnotation
persistent identity: annotation301166
display id: annotation301166
version: 1
top level: OmpR
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0082/annotation301166/range301166/1

https://synbiohub.org/public/igem/BBa_R0082/annotation301166/range301166/1
type: Range
persistent identity: range301166
display id: range301166
version: 1
direction: direction/forward
top level: OmpR
start: 75
end: 80
orientation: inline

C3 OmpR
type: SequenceAnnotation
persistent identity: annotation301156
display id: annotation301156
version: 1
top level: OmpR
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0082/annotation301156/range301156/1

https://synbiohub.org/public/igem/BBa_R0082/annotation301156/range301156/1
type: Range
persistent identity: range301156
display id: range301156
version: 1
direction: direction/forward
top level: OmpR
start: 54
end: 71
orientation: inline

C1 OmpR
type: SequenceAnnotation
persistent identity: annotation301154
display id: annotation301154
version: 1
top level: OmpR
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0082/annotation301154/range301154/1

https://synbiohub.org/public/igem/BBa_R0082/annotation301154/range301154/1
type: Range
persistent identity: range301154
display id: range301154
version: 1
direction: direction/forward
top level: OmpR
start: 13
end: 30
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation301167
display id: annotation301167
version: 1
top level: OmpR
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0082/annotation301167/range301167/1

https://synbiohub.org/public/igem/BBa_R0082/annotation301167/range301167/1
type: Range
persistent identity: range301167
display id: range301167
version: 1
direction: direction/forward
top level: OmpR
start: 98
end: 103
orientation: inline

C2 OmpR
type: SequenceAnnotation
persistent identity: annotation301155
display id: annotation301155
version: 1
top level: OmpR
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0082/annotation301155/range301155/1

https://synbiohub.org/public/igem/BBa_R0082/annotation301155/range301155/1
type: Range
persistent identity: range301155
display id: range301155
version: 1
direction: direction/forward
top level: OmpR
start: 34
end: 51
orientation: inline

U061O11+D0
type: ComponentDefinition
persistent identity: BBa I732431
display id: BBa_I732431
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member (U061O11+D002O22)
creator: Zhan Jian
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732431_sequence/1

BBa_K819007
type: ComponentDefinition
persistent identity: BBa K819007
display id: BBa_K819007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Measurement device for Luminesensor
creator: YU Zhou
created: 2012-09-02T11:00:00Z
modified: 2015-05-08T01:13:29Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1077_
m user id: 0
owner id: 13785
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: recA408 Promoter + B0030 + GFP + ssrA-tag
mutable notes: recA408 Promoter + B0030 + GFP + ssrA-tag
mutable provenance: From Parts Registry
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: RecA408 Promoter
sequence annotation: RBS-GFP-ssrA:BBa_K581007
sequence: https://synbiohub.org/public/igem/BBa_K819007_sequence/1

RecA408 Promoter
type: SequenceAnnotation
persistent identity: annotation2197244
display id: annotation2197244
version: 1
top level: BBa_K819007
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K819007/annotation2197244/range2197244/1

https://synbiohub.org/public/igem/BBa_K819007/annotation2197244/range2197244/1
type: Range
persistent identity: range2197244
display id: range2197244
version: 1
direction: direction/forward
top level: BBa_K819007
start: 1
end: 79
orientation: inline

RBS-GFP-ssrA:BBa_K581007
type: SequenceAnnotation
persistent identity: annotation2197250
display id: annotation2197250
version: 1
top level: BBa_K819007
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K819007/annotation2197250/range2197250/1

https://synbiohub.org/public/igem/BBa_K819007/annotation2197250/range2197250/1
type: Range
persistent identity: range2197250
display id: range2197250
version: 1
direction: direction/forward
top level: BBa_K819007
start: 80
end: 857
orientation: inline

revPlam
type: ComponentDefinition
persistent identity: BBa I742126
display id: BBa_I742126
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Reverse lambda cI-regulated promoter
creator: Caroline Dahl
created: 2007-10-21T11:00:00Z
modified: 2015-08-31T04:08:02Z
discontinued: false
dominant: false
experience: experience/None
group u list: _123_
m user id: 0
owner id: 1968
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is an exact 'upside down' version of BBa_R0051. The promoter has two DNA binding sites for lambda cI repressor BBa_C0051. By adding this part, we wish to emphasise the possibility of engineering both DNA strands, and the additional regulatory potential that it gives.
mutable notes: Any 'upside down' (reverse complementary) sequences made by PCR amplification need be made with suffix and prefix is opposite order with respect to the original default sequence.
mutable provenance: See BBa_R0051.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I742126_sequence/1

BBa_K091110
type: ComponentDefinition
persistent identity: BBa K091110
display id: BBa_K091110
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LacI Promoter
creator: Robert Cool
created: 2008-06-19T11:00:00Z
modified: 2015-05-08T01:08:37Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _191_
m user id: 0
owner id: 3080
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This is a modified version of the LacI promoter that should bind LacI tighter than the original.
mutable notes: no special considerations
mutable provenance: This promoter is a modified version of the LacI promoter
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -35
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_K091110_sequence/1

-35
type: SequenceAnnotation
persistent identity: annotation1964081
display id: annotation1964081
version: 1
top level: BBa_K091110
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K091110/annotation1964081/range1964081/1

https://synbiohub.org/public/igem/BBa_K091110/annotation1964081/range1964081/1
type: Range
persistent identity: range1964081
display id: range1964081
version: 1
direction: direction/forward
top level: BBa_K091110
start: 17
end: 22
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1964082
display id: annotation1964082
version: 1
top level: BBa_K091110
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K091110/annotation1964082/range1964082/1

https://synbiohub.org/public/igem/BBa_K091110/annotation1964082/range1964082/1
type: Range
persistent identity: range1964082
display id: range1964082
version: 1
direction: direction/forward
top level: BBa_K091110
start: 44
end: 49
orientation: inline

BBa_I751501
type: ComponentDefinition
persistent identity: BBa I751501
display id: BBa_I751501
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: plux-cI hybrid promoter
creator: Kenichiro Iwasaki
created: 2007-10-24T11:00:00Z
modified: 2015-08-31T04:08:06Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _151_
m user id: 0
owner id: 2188
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: sense TWO INPUTS, activation by AHL and repression by LacI.
mutable notes: Based on the AHL regulated BioBrick part R0062 and the previous work on LacI regulated promoter (ref. 1), we designed a new promoter regulated by AHL and LacI. Since this promoter is a kind of a "HYBRID" of the two, we call it HYBRID PROMOTER. Compared with existing BioBrick hybrid promoter R0065, this promoter is different in that its sequence is much shorter with lux box and -35 region overlapping, and that it is repressed by LacI, not cI. Also, the output is clearly detected as shown in Fig. 3.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: plux-cI hybrid promoter
sequence annotation: luxR binding
sequence annotation: -35
sequence annotation: -10
sequence annotation: OR1
sequence annotation: OR2
sequence: https://synbiohub.org/public/igem/BBa_I751501_sequence/1

plux-cI hybrid promoter
type: SequenceAnnotation
persistent identity: annotation1955478
display id: annotation1955478
version: 1
top level: BBa_I751501
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I751501/annotation1955478/range1955478/1

https://synbiohub.org/public/igem/BBa_I751501/annotation1955478/range1955478/1
type: Range
persistent identity: range1955478
display id: range1955478
version: 1
direction: direction/forward
top level: BBa_I751501
start: 19
end: 66
orientation: inline

luxR binding
type: SequenceAnnotation
persistent identity: annotation1955482
display id: annotation1955482
version: 1
top level: BBa_I751501
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I751501/annotation1955482/range1955482/1

https://synbiohub.org/public/igem/BBa_I751501/annotation1955482/range1955482/1
type: Range
persistent identity: range1955482
display id: range1955482
version: 1
direction: direction/forward
top level: BBa_I751501
start: 1
end: 18
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1955479
display id: annotation1955479
version: 1
top level: BBa_I751501
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I751501/annotation1955479/range1955479/1

https://synbiohub.org/public/igem/BBa_I751501/annotation1955479/range1955479/1
type: Range
persistent identity: range1955479
display id: range1955479
version: 1
direction: direction/forward
top level: BBa_I751501
start: 19
end: 25
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1955480
display id: annotation1955480
version: 1
top level: BBa_I751501
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I751501/annotation1955480/range1955480/1

https://synbiohub.org/public/igem/BBa_I751501/annotation1955480/range1955480/1
type: Range
persistent identity: range1955480
display id: range1955480
version: 1
direction: direction/forward
top level: BBa_I751501
start: 42
end: 47
orientation: inline

OR1
type: SequenceAnnotation
persistent identity: annotation1955477
display id: annotation1955477
version: 1
top level: BBa_I751501
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I751501/annotation1955477/range1955477/1

https://synbiohub.org/public/igem/BBa_I751501/annotation1955477/range1955477/1
type: Range
persistent identity: range1955477
display id: range1955477
version: 1
direction: direction/forward
top level: BBa_I751501
start: 48
end: 66
orientation: inline

OR2
type: SequenceAnnotation
persistent identity: annotation1955481
display id: annotation1955481
version: 1
top level: BBa_I751501
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I751501/annotation1955481/range1955481/1

https://synbiohub.org/public/igem/BBa_I751501/annotation1955481/range1955481/1
type: Range
persistent identity: range1955481
display id: range1955481
version: 1
direction: direction/forward
top level: BBa_I751501
start: 26
end: 41
orientation: inline

U061O11+D0
type: ComponentDefinition
persistent identity: BBa I732436
display id: BBa_I732436
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member (U061O11+D062O22)
creator: Zhan Jian
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732436_sequence/1

TetR-4C
type: ComponentDefinition
persistent identity: BBa I12212
display id: BBa_I12212
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TetR - TetR-4C heterodimer promoter (negative)
creator: rwald
created: 2004-08-05T11:00:00Z
modified: 2015-08-31T04:07:31Z
discontinued: false
dominant: false
experience: experience/None
group u list: _3_
m user id: 0
owner id: 120
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This promoter is repressed by a heterodimer of wild-type TetR and mutant TetR-4C. It differs from wild-type TetR promoter in that the +4 and -4 regions of its O2 binding site have been changed, and the -35 region of the reverse promoter (pA) has been destroyed.
mutable notes: The O2 and O1 mutations are all single point mutations, occuring at the first base which appears listed here.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -10
sequence annotation: O1
sequence annotation: O1 mutation
sequence annotation: -35
sequence annotation: TetR O2
sequence annotation: O2 mutation
sequence: https://synbiohub.org/public/igem/BBa_I12212_sequence/1

-10
type: SequenceAnnotation
persistent identity: annotation1031340
display id: annotation1031340
version: 1
top level: TetR-4C
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I12212/annotation1031340/range1031340/1

https://synbiohub.org/public/igem/BBa_I12212/annotation1031340/range1031340/1
type: Range
persistent identity: range1031340
display id: range1031340
version: 1
direction: direction/forward
top level: TetR-4C
start: 26
end: 30
orientation: inline

O1
type: SequenceAnnotation
persistent identity: annotation1031341
display id: annotation1031341
version: 1
top level: TetR-4C
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I12212/annotation1031341/range1031341/1

https://synbiohub.org/public/igem/BBa_I12212/annotation1031341/range1031341/1
type: Range
persistent identity: range1031341
display id: range1031341
version: 1
direction: direction/forward
top level: TetR-4C
start: 32
end: 50
orientation: inline

O1 mutation
type: SequenceAnnotation
persistent identity: annotation1057574
display id: annotation1057574
version: 1
top level: TetR-4C
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I12212/annotation1057574/range1057574/1

https://synbiohub.org/public/igem/BBa_I12212/annotation1057574/range1057574/1
type: Range
persistent identity: range1057574
display id: range1057574
version: 1
direction: direction/forward
top level: TetR-4C
start: 37
end: 37
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1031342
display id: annotation1031342
version: 1
top level: TetR-4C
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I12212/annotation1031342/range1031342/1

https://synbiohub.org/public/igem/BBa_I12212/annotation1031342/range1031342/1
type: Range
persistent identity: range1031342
display id: range1031342
version: 1
direction: direction/forward
top level: TetR-4C
start: 45
end: 50
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1031337
display id: annotation1031337
version: 1
top level: TetR-4C
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I12212/annotation1031337/range1031337/1

https://synbiohub.org/public/igem/BBa_I12212/annotation1031337/range1031337/1
type: Range
persistent identity: range1031337
display id: range1031337
version: 1
direction: direction/forward
top level: TetR-4C
start: 1
end: 6
orientation: inline

TetR O2
type: SequenceAnnotation
persistent identity: annotation1031338
display id: annotation1031338
version: 1
top level: TetR-4C
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I12212/annotation1031338/range1031338/1

https://synbiohub.org/public/igem/BBa_I12212/annotation1031338/range1031338/1
type: Range
persistent identity: range1031338
display id: range1031338
version: 1
direction: direction/forward
top level: TetR-4C
start: 2
end: 20
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1031339
display id: annotation1031339
version: 1
top level: TetR-4C
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I12212/annotation1031339/range1031339/1

https://synbiohub.org/public/igem/BBa_I12212/annotation1031339/range1031339/1
type: Range
persistent identity: range1031339
display id: range1031339
version: 1
direction: direction/forward
top level: TetR-4C
start: 22
end: 27
orientation: inline

O2 mutation
type: SequenceAnnotation
persistent identity: annotation1057573
display id: annotation1057573
version: 1
top level: TetR-4C
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I12212/annotation1057573/range1057573/1

https://synbiohub.org/public/igem/BBa_I12212/annotation1057573/range1057573/1
type: Range
persistent identity: range1057573
display id: range1057573
version: 1
direction: direction/forward
top level: TetR-4C
start: 7
end: 7
orientation: inline

U049O11+D0
type: ComponentDefinition
persistent identity: BBa I732405
display id: BBa_I732405
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member (U049O11+D062NUL)
creator: Zhan Jian
created: 2007-09-19T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1572
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: A Double Reporter (LacZ-alpha and GFP-AAV) loaded on pSB3K5. For more information please refer to BBa_I732402.
mutable notes: Might be later edited.
mutable provenance: Might be later edited.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BglII
sequence annotation: STOP
sequence annotation: stem_loop
sequence annotation: BBa_B0034
sequence annotation: BBa_B0012
sequence annotation: BBa_E1010
sequence annotation: lacZ
sequence annotation: BBa_I732006
sequence annotation: BBa_B0010
sequence annotation: barcode
sequence annotation: mrfp1
sequence annotation: BBa_I732007
sequence annotation: BamHI
sequence annotation: stop
sequence annotation: polya
sequence annotation: START
sequence annotation: T7 TE
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_I732405_sequence/1

BglII
type: SequenceAnnotation
persistent identity: annotation1944404
display id: annotation1944404
version: 1
top level: U049O11+D0
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I732405/annotation1944404/range1944404/1

https://synbiohub.org/public/igem/BBa_I732405/annotation1944404/range1944404/1
type: Range
persistent identity: range1944404
display id: range1944404
version: 1
direction: direction/forward
top level: U049O11+D0
start: 3
end: 8
orientation: inline

STOP
type: SequenceAnnotation
persistent identity: annotation1944411
display id: annotation1944411
version: 1
top level: U049O11+D0
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_I732405/annotation1944411/range1944411/1

https://synbiohub.org/public/igem/BBa_I732405/annotation1944411/range1944411/1
type: Range
persistent identity: range1944411
display id: range1944411
version: 1
direction: direction/forward
top level: U049O11+D0
start: 274
end: 279
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation1944419
display id: annotation1944419
version: 1
top level: U049O11+D0
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_I732405/annotation1944419/range1944419/1

https://synbiohub.org/public/igem/BBa_I732405/annotation1944419/range1944419/1
type: Range
persistent identity: range1944419
display id: range1944419
version: 1
direction: direction/forward
top level: U049O11+D0
start: 1031
end: 1074
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1944408
display id: annotation1944408
version: 1
top level: U049O11+D0
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I732405/annotation1944408/range1944408/1

https://synbiohub.org/public/igem/BBa_I732405/annotation1944408/range1944408/1
type: Range
persistent identity: range1944408
display id: range1944408
version: 1
direction: direction/reverse
top level: U049O11+D0
start: 28
end: 39
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1944420
display id: annotation1944420
version: 1
top level: U049O11+D0
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I732405/annotation1944420/range1944420/1

https://synbiohub.org/public/igem/BBa_I732405/annotation1944420/range1944420/1
type: Range
persistent identity: range1944420
display id: range1944420
version: 1
direction: direction/forward
top level: U049O11+D0
start: 1108
end: 1148
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1944414
display id: annotation1944414
version: 1
top level: U049O11+D0
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I732405/annotation1944414/range1944414/1

https://synbiohub.org/public/igem/BBa_I732405/annotation1944414/range1944414/1
type: Range
persistent identity: range1944414
display id: range1944414
version: 1
direction: direction/reverse
top level: U049O11+D0
start: 288
end: 299
orientation: inline

BBa_E1010
type: SequenceAnnotation
persistent identity: annotation1944416
display id: annotation1944416
version: 1
top level: U049O11+D0
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I732405/annotation1944416/range1944416/1

https://synbiohub.org/public/igem/BBa_I732405/annotation1944416/range1944416/1
type: Range
persistent identity: range1944416
display id: range1944416
version: 1
direction: direction/reverse
top level: U049O11+D0
start: 306
end: 986
orientation: inline

lacZ
type: SequenceAnnotation
persistent identity: annotation1944410
display id: annotation1944410
version: 1
top level: U049O11+D0
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_I732405/annotation1944410/range1944410/1

https://synbiohub.org/public/igem/BBa_I732405/annotation1944410/range1944410/1
type: Range
persistent identity: range1944410
display id: range1944410
version: 1
direction: direction/forward
top level: U049O11+D0
start: 46
end: 279
orientation: inline

BBa_I732006
type: SequenceAnnotation
persistent identity: annotation1944412
display id: annotation1944412
version: 1
top level: U049O11+D0
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I732405/annotation1944412/range1944412/1

https://synbiohub.org/public/igem/BBa_I732405/annotation1944412/range1944412/1
type: Range
persistent identity: range1944412
display id: range1944412
version: 1
direction: direction/reverse
top level: U049O11+D0
start: 46
end: 279
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1944418
display id: annotation1944418
version: 1
top level: U049O11+D0
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I732405/annotation1944418/range1944418/1

https://synbiohub.org/public/igem/BBa_I732405/annotation1944418/range1944418/1
type: Range
persistent identity: range1944418
display id: range1944418
version: 1
direction: direction/forward
top level: U049O11+D0
start: 1020
end: 1099
orientation: inline

barcode
type: SequenceAnnotation
persistent identity: annotation1944417
display id: annotation1944417
version: 1
top level: U049O11+D0
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I732405/annotation1944417/range1944417/1

https://synbiohub.org/public/igem/BBa_I732405/annotation1944417/range1944417/1
type: Range
persistent identity: range1944417
display id: range1944417
version: 1
direction: direction/reverse
top level: U049O11+D0
start: 987
end: 1011
orientation: inline

mrfp1
type: SequenceAnnotation
persistent identity: annotation1944415
display id: annotation1944415
version: 1
top level: U049O11+D0
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_I732405/annotation1944415/range1944415/1

https://synbiohub.org/public/igem/BBa_I732405/annotation1944415/range1944415/1
type: Range
persistent identity: range1944415
display id: range1944415
version: 1
direction: direction/forward
top level: U049O11+D0
start: 306
end: 980
orientation: inline

BBa_I732007
type: SequenceAnnotation
persistent identity: annotation1944406
display id: annotation1944406
version: 1
top level: U049O11+D0
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I732405/annotation1944406/range1944406/1

https://synbiohub.org/public/igem/BBa_I732405/annotation1944406/range1944406/1
type: Range
persistent identity: range1944406
display id: range1944406
version: 1
direction: direction/reverse
top level: U049O11+D0
start: 1
end: 19
orientation: inline

BamHI
type: SequenceAnnotation
persistent identity: annotation1944405
display id: annotation1944405
version: 1
top level: U049O11+D0
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I732405/annotation1944405/range1944405/1

https://synbiohub.org/public/igem/BBa_I732405/annotation1944405/range1944405/1
type: Range
persistent identity: range1944405
display id: range1944405
version: 1
direction: direction/forward
top level: U049O11+D0
start: 12
end: 17
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1944423
display id: annotation1944423
version: 1
top level: U049O11+D0
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_I732405/annotation1944423/range1944423/1

https://synbiohub.org/public/igem/BBa_I732405/annotation1944423/range1944423/1
type: Range
persistent identity: range1944423
display id: range1944423
version: 1
direction: direction/forward
top level: U049O11+D0
start: 1141
end: 1141
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1944422
display id: annotation1944422
version: 1
top level: U049O11+D0
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_I732405/annotation1944422/range1944422/1

https://synbiohub.org/public/igem/BBa_I732405/annotation1944422/range1944422/1
type: Range
persistent identity: range1944422
display id: range1944422
version: 1
direction: direction/forward
top level: U049O11+D0
start: 1135
end: 1148
orientation: inline

START
type: SequenceAnnotation
persistent identity: annotation1944409
display id: annotation1944409
version: 1
top level: U049O11+D0
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_I732405/annotation1944409/range1944409/1

https://synbiohub.org/public/igem/BBa_I732405/annotation1944409/range1944409/1
type: Range
persistent identity: range1944409
display id: range1944409
version: 1
direction: direction/forward
top level: U049O11+D0
start: 46
end: 48
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1944421
display id: annotation1944421
version: 1
top level: U049O11+D0
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_I732405/annotation1944421/range1944421/1

https://synbiohub.org/public/igem/BBa_I732405/annotation1944421/range1944421/1
type: Range
persistent identity: range1944421
display id: range1944421
version: 1
direction: direction/forward
top level: U049O11+D0
start: 1115
end: 1134
orientation: inline

conserved
type: SequenceAnnotation
persistent identity: annotation1944407
display id: annotation1944407
version: 1
top level: U049O11+D0
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_I732405/annotation1944407/range1944407/1

https://synbiohub.org/public/igem/BBa_I732405/annotation1944407/range1944407/1
type: Range
persistent identity: range1944407
display id: range1944407
version: 1
direction: direction/forward
top level: U049O11+D0
start: 32
end: 35
orientation: inline

conserved
type: SequenceAnnotation
persistent identity: annotation1944413
display id: annotation1944413
version: 1
top level: U049O11+D0
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_I732405/annotation1944413/range1944413/1

https://synbiohub.org/public/igem/BBa_I732405/annotation1944413/range1944413/1
type: Range
persistent identity: range1944413
display id: range1944413
version: 1
direction: direction/forward
top level: U049O11+D0
start: 292
end: 295
orientation: inline

BBa_K091156
type: ComponentDefinition
persistent identity: BBa K091156
display id: BBa_K091156
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pLux
creator: Erin Feeney
created: 2008-07-24T11:00:00Z
modified: 2015-05-08T01:08:38Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _191_
m user id: 0
owner id: 3067
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: The pLux' promoter is a modified version of BBa_R0062 containing the -35 region TTTAAT in place of TTTACG. Transcriptional activation is expected to occur in the presence of LuxR and AI-1, just as with R0062. The modified -35 region was designed to serve as a control for the evaluation of BBa_K091156, the pLux+ Las- promoter.
mutable notes: This promoter is identical to pLux+ Las- with the exception of the Las box's absence
mutable provenance: This part was adapted from BBa_R0062.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -35 Region
sequence annotation: LuxR
sequence annotation: -10 Region
sequence: https://synbiohub.org/public/igem/BBa_K091156_sequence/1

-35 Region
type: SequenceAnnotation
persistent identity: annotation1968420
display id: annotation1968420
version: 1
top level: BBa_K091156
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K091156/annotation1968420/range1968420/1

https://synbiohub.org/public/igem/BBa_K091156/annotation1968420/range1968420/1
type: Range
persistent identity: range1968420
display id: range1968420
version: 1
direction: direction/forward
top level: BBa_K091156
start: 20
end: 25
orientation: inline

LuxR
type: SequenceAnnotation
persistent identity: annotation1968422
display id: annotation1968422
version: 1
top level: BBa_K091156
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K091156/annotation1968422/range1968422/1

https://synbiohub.org/public/igem/BBa_K091156/annotation1968422/range1968422/1
type: Range
persistent identity: range1968422
display id: range1968422
version: 1
direction: direction/forward
top level: BBa_K091156
start: 1
end: 20
orientation: inline

-10 Region
type: SequenceAnnotation
persistent identity: annotation1968421
display id: annotation1968421
version: 1
top level: BBa_K091156
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K091156/annotation1968421/range1968421/1

https://synbiohub.org/public/igem/BBa_K091156/annotation1968421/range1968421/1
type: Range
persistent identity: range1968421
display id: range1968421
version: 1
direction: direction/forward
top level: BBa_K091156
start: 42
end: 47
orientation: inline

U085O11+D0
type: ComponentDefinition
persistent identity: BBa I732423
display id: BBa_I732423
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member (U085O11+D050O22)
creator: Zhan Jian
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732423_sequence/1

BBa_J16101
type: ComponentDefinition
persistent identity: BBa J16101
display id: BBa_J16101
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: BanAp - Banana-induced Promoter
creator: James Brown
created: 2006-07-23T11:00:00Z
modified: 2015-08-31T04:08:33Z
discontinued: false
dominant: false
group u list: _48_
m user id: 0
owner id: 740
owning group id: 1
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Taken from no other than Curious George, this promoter is induced by the presence of banana in the metabolism of any mammal. It is very weak however without the presence of the HngR protein. For transcription to reach significant levels both HngR and banana must be present
mutable notes: Monkeys can be dangerous when aggrevated
mutable provenance: Curious George's very own DNA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J16101_sequence/1

BBa_K180004
type: ComponentDefinition
persistent identity: BBa K180004
display id: BBa_K180004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Game of Life - Primary plasmid
creator: Richard Wilson, Stephanie Chambers
created: 2009-10-15T11:00:00Z
modified: 2015-05-08T01:11:06Z
discontinued: false
dominant: false
experience: experience/None
group u list: _278_
m user id: 0
owner id: 4082
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: Composite part, consisting of BBa_K180005 and BBa_K180006, but with BBa_K180005 reading along reverse strand, and BBa_K180006 reading along the forward strand.
mutable notes: BBa_K180005 reading along reverse strand, and BBa_K180006 reading along the forward strand to ensure the constitutive promoter BBa_J23119 (present in BBa_K180005) has no effect over LacI or GFP-LVA expression in BBa_K180006.
mutable provenance: See relevant biobrick component parts (BBa_K180005 and BBa_K180006)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: B0015
sequence annotation: start
sequence annotation: -35
sequence annotation: -10
sequence annotation: R0062
sequence annotation: C0012
sequence annotation: C0062
sequence annotation: lacI
sequence annotation: conserved
sequence annotation: LVA
sequence annotation: GFPmut3b
sequence annotation: K180006
sequence annotation: J23119
sequence annotation: B0034
sequence annotation: LuxR/HSL
sequence annotation: K180001
sequence annotation: luxR
sequence annotation: K180005
sequence: https://synbiohub.org/public/igem/BBa_K180004_sequence/1

B0015
type: SequenceAnnotation
persistent identity: annotation2058522
display id: annotation2058522
version: 1
top level: BBa_K180004
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K180004/annotation2058522/range2058522/1

https://synbiohub.org/public/igem/BBa_K180004/annotation2058522/range2058522/1
type: Range
persistent identity: range2058522
display id: range2058522
version: 1
direction: direction/reverse
top level: BBa_K180004
start: 1
end: 129
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2058534
display id: annotation2058534
version: 1
top level: BBa_K180004
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K180004/annotation2058534/range2058534/1

https://synbiohub.org/public/igem/BBa_K180004/annotation2058534/range2058534/1
type: Range
persistent identity: range2058534
display id: range2058534
version: 1
direction: direction/forward
top level: BBa_K180004
start: 1040
end: 1040
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2058546
display id: annotation2058546
version: 1
top level: BBa_K180004
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K180004/annotation2058546/range2058546/1

https://synbiohub.org/public/igem/BBa_K180004/annotation2058546/range2058546/1
type: Range
persistent identity: range2058546
display id: range2058546
version: 1
direction: direction/forward
top level: BBa_K180004
start: 1007
end: 1012
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2058530
display id: annotation2058530
version: 1
top level: BBa_K180004
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K180004/annotation2058530/range2058530/1

https://synbiohub.org/public/igem/BBa_K180004/annotation2058530/range2058530/1
type: Range
persistent identity: range2058530
display id: range2058530
version: 1
direction: direction/reverse
top level: BBa_K180004
start: 974
end: 979
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2058533
display id: annotation2058533
version: 1
top level: BBa_K180004
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K180004/annotation2058533/range2058533/1

https://synbiohub.org/public/igem/BBa_K180004/annotation2058533/range2058533/1
type: Range
persistent identity: range2058533
display id: range2058533
version: 1
direction: direction/forward
top level: BBa_K180004
start: 1029
end: 1034
orientation: inline

R0062
type: SequenceAnnotation
persistent identity: annotation2058531
display id: annotation2058531
version: 1
top level: BBa_K180004
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K180004/annotation2058531/range2058531/1

https://synbiohub.org/public/igem/BBa_K180004/annotation2058531/range2058531/1
type: Range
persistent identity: range2058531
display id: range2058531
version: 1
direction: direction/forward
top level: BBa_K180004
start: 988
end: 1042
orientation: inline

C0012
type: SequenceAnnotation
persistent identity: annotation2058535
display id: annotation2058535
version: 1
top level: BBa_K180004
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K180004/annotation2058535/range2058535/1

https://synbiohub.org/public/igem/BBa_K180004/annotation2058535/range2058535/1
type: Range
persistent identity: range2058535
display id: range2058535
version: 1
direction: direction/forward
top level: BBa_K180004
start: 1069
end: 2196
orientation: inline

C0062
type: SequenceAnnotation
persistent identity: annotation2058523
display id: annotation2058523
version: 1
top level: BBa_K180004
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K180004/annotation2058523/range2058523/1

https://synbiohub.org/public/igem/BBa_K180004/annotation2058523/range2058523/1
type: Range
persistent identity: range2058523
display id: range2058523
version: 1
direction: direction/reverse
top level: BBa_K180004
start: 163
end: 918
orientation: inline

lacI
type: SequenceAnnotation
persistent identity: annotation2058536
display id: annotation2058536
version: 1
top level: BBa_K180004
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K180004/annotation2058536/range2058536/1

https://synbiohub.org/public/igem/BBa_K180004/annotation2058536/range2058536/1
type: Range
persistent identity: range2058536
display id: range2058536
version: 1
direction: direction/forward
top level: BBa_K180004
start: 1069
end: 2157
orientation: inline

conserved
type: SequenceAnnotation
persistent identity: annotation2058538
display id: annotation2058538
version: 1
top level: BBa_K180004
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K180004/annotation2058538/range2058538/1

https://synbiohub.org/public/igem/BBa_K180004/annotation2058538/range2058538/1
type: Range
persistent identity: range2058538
display id: range2058538
version: 1
direction: direction/forward
top level: BBa_K180004
start: 1055
end: 1058
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation2058544
display id: annotation2058544
version: 1
top level: BBa_K180004
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K180004/annotation2058544/range2058544/1

https://synbiohub.org/public/igem/BBa_K180004/annotation2058544/range2058544/1
type: Range
persistent identity: range2058544
display id: range2058544
version: 1
direction: direction/forward
top level: BBa_K180004
start: 2968
end: 3001
orientation: inline

conserved
type: SequenceAnnotation
persistent identity: annotation2058543
display id: annotation2058543
version: 1
top level: BBa_K180004
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K180004/annotation2058543/range2058543/1

https://synbiohub.org/public/igem/BBa_K180004/annotation2058543/range2058543/1
type: Range
persistent identity: range2058543
display id: range2058543
version: 1
direction: direction/forward
top level: BBa_K180004
start: 2234
end: 2237
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2058528
display id: annotation2058528
version: 1
top level: BBa_K180004
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K180004/annotation2058528/range2058528/1

https://synbiohub.org/public/igem/BBa_K180004/annotation2058528/range2058528/1
type: Range
persistent identity: range2058528
display id: range2058528
version: 1
direction: direction/reverse
top level: BBa_K180004
start: 951
end: 956
orientation: inline

GFPmut3b
type: SequenceAnnotation
persistent identity: annotation2058540
display id: annotation2058540
version: 1
top level: BBa_K180004
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K180004/annotation2058540/range2058540/1

https://synbiohub.org/public/igem/BBa_K180004/annotation2058540/range2058540/1
type: Range
persistent identity: range2058540
display id: range2058540
version: 1
direction: direction/forward
top level: BBa_K180004
start: 2248
end: 2967
orientation: inline

K180006
type: SequenceAnnotation
persistent identity: annotation2058532
display id: annotation2058532
version: 1
top level: BBa_K180004
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K180004/annotation2058532/range2058532/1

https://synbiohub.org/public/igem/BBa_K180004/annotation2058532/range2058532/1
type: Range
persistent identity: range2058532
display id: range2058532
version: 1
direction: direction/forward
top level: BBa_K180004
start: 988
end: 3138
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation2058539
display id: annotation2058539
version: 1
top level: BBa_K180004
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K180004/annotation2058539/range2058539/1

https://synbiohub.org/public/igem/BBa_K180004/annotation2058539/range2058539/1
type: Range
persistent identity: range2058539
display id: range2058539
version: 1
direction: direction/forward
top level: BBa_K180004
start: 2158
end: 2196
orientation: inline

J23119
type: SequenceAnnotation
persistent identity: annotation2058527
display id: annotation2058527
version: 1
top level: BBa_K180004
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K180004/annotation2058527/range2058527/1

https://synbiohub.org/public/igem/BBa_K180004/annotation2058527/range2058527/1
type: Range
persistent identity: range2058527
display id: range2058527
version: 1
direction: direction/reverse
top level: BBa_K180004
start: 945
end: 979
orientation: inline

conserved
type: SequenceAnnotation
persistent identity: annotation2058526
display id: annotation2058526
version: 1
top level: BBa_K180004
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K180004/annotation2058526/range2058526/1

https://synbiohub.org/public/igem/BBa_K180004/annotation2058526/range2058526/1
type: Range
persistent identity: range2058526
display id: range2058526
version: 1
direction: direction/reverse
top level: BBa_K180004
start: 929
end: 932
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation2058525
display id: annotation2058525
version: 1
top level: BBa_K180004
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K180004/annotation2058525/range2058525/1

https://synbiohub.org/public/igem/BBa_K180004/annotation2058525/range2058525/1
type: Range
persistent identity: range2058525
display id: range2058525
version: 1
direction: direction/reverse
top level: BBa_K180004
start: 925
end: 936
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation2058537
display id: annotation2058537
version: 1
top level: BBa_K180004
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K180004/annotation2058537/range2058537/1

https://synbiohub.org/public/igem/BBa_K180004/annotation2058537/range2058537/1
type: Range
persistent identity: range2058537
display id: range2058537
version: 1
direction: direction/forward
top level: BBa_K180004
start: 1051
end: 1062
orientation: inline

LuxR/HSL
type: SequenceAnnotation
persistent identity: annotation2058529
display id: annotation2058529
version: 1
top level: BBa_K180004
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K180004/annotation2058529/range2058529/1

https://synbiohub.org/public/igem/BBa_K180004/annotation2058529/range2058529/1
type: Range
persistent identity: range2058529
display id: range2058529
version: 1
direction: direction/forward
top level: BBa_K180004
start: 988
end: 1007
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation2058542
display id: annotation2058542
version: 1
top level: BBa_K180004
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K180004/annotation2058542/range2058542/1

https://synbiohub.org/public/igem/BBa_K180004/annotation2058542/range2058542/1
type: Range
persistent identity: range2058542
display id: range2058542
version: 1
direction: direction/forward
top level: BBa_K180004
start: 2230
end: 2241
orientation: inline

K180001
type: SequenceAnnotation
persistent identity: annotation2058541
display id: annotation2058541
version: 1
top level: BBa_K180004
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K180004/annotation2058541/range2058541/1

https://synbiohub.org/public/igem/BBa_K180004/annotation2058541/range2058541/1
type: Range
persistent identity: range2058541
display id: range2058541
version: 1
direction: direction/forward
top level: BBa_K180004
start: 2248
end: 3001
orientation: inline

B0015
type: SequenceAnnotation
persistent identity: annotation2058545
display id: annotation2058545
version: 1
top level: BBa_K180004
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K180004/annotation2058545/range2058545/1

https://synbiohub.org/public/igem/BBa_K180004/annotation2058545/range2058545/1
type: Range
persistent identity: range2058545
display id: range2058545
version: 1
direction: direction/forward
top level: BBa_K180004
start: 3010
end: 3138
orientation: inline

luxR
type: SequenceAnnotation
persistent identity: annotation2058524
display id: annotation2058524
version: 1
top level: BBa_K180004
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K180004/annotation2058524/range2058524/1

https://synbiohub.org/public/igem/BBa_K180004/annotation2058524/range2058524/1
type: Range
persistent identity: range2058524
display id: range2058524
version: 1
direction: direction/reverse
top level: BBa_K180004
start: 163
end: 912
orientation: inline

K180005
type: SequenceAnnotation
persistent identity: annotation2058521
display id: annotation2058521
version: 1
top level: BBa_K180004
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K180004/annotation2058521/range2058521/1

https://synbiohub.org/public/igem/BBa_K180004/annotation2058521/range2058521/1
type: Range
persistent identity: range2058521
display id: range2058521
version: 1
direction: direction/reverse
top level: BBa_K180004
start: 1
end: 979
orientation: inline

BBa_K266009
type: ComponentDefinition
persistent identity: BBa K266009
display id: BBa_K266009
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: J23100 + Lac inverter + RBS
creator: UNAM FCIENCIAS-CINVESTAV IRA
created: 2009-10-12T11:00:00Z
modified: 2015-05-08T01:11:43Z
discontinued: false
dominant: false
experience: experience/None
group u list: _363_
m user id: 0
owner id: 1955
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: The BBa_J23100 constitutive promoter with Lac systems inverter and strong RBS
mutable notes: None
mutable provenance: The biobricks BBa_K266008 and BBa_B0034 where used to build this part
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Inverter
component: BBa_K266008
component: BBa_B0034
sequence annotation: BBa_B0034
sequence annotation: BBa_K266008
sequence: https://synbiohub.org/public/igem/BBa_K266009_sequence/1

BBa_K266008
type: Component
persistent identity: component2267639
display id: component2267639
version: 1
top level: BBa_K266009
access: public
definition: BBa_K266008

BBa_B0034
type: Component
persistent identity: component2267641
display id: component2267641
version: 1
top level: BBa_K266009
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2267641
display id: annotation2267641
version: 1
top level: BBa_K266009
location: https://synbiohub.org/public/igem/BBa_K266009/annotation2267641/range2267641/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K266009/annotation2267641/range2267641/1
type: Range
persistent identity: range2267641
display id: range2267641
version: 1
direction: direction/reverse
top level: BBa_K266009
start: 1423
end: 1434
orientation: inline

BBa_K266008
type: SequenceAnnotation
persistent identity: annotation2267639
display id: annotation2267639
version: 1
top level: BBa_K266009
location: https://synbiohub.org/public/igem/BBa_K266009/annotation2267639/range2267639/1
component: BBa_K266008

https://synbiohub.org/public/igem/BBa_K266009/annotation2267639/range2267639/1
type: Range
persistent identity: range2267639
display id: range2267639
version: 1
direction: direction/reverse
top level: BBa_K266009
start: 1
end: 1414
orientation: inline

BBa_K086017
type: ComponentDefinition
persistent identity: BBa K086017
display id: BBa_K086017
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: unmodified Lutz-Bujard LacO promoter
creator: Gairik Sachdeva
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:08:36Z
discontinued: false
dominant: false
group u list: _197_
m user id: 0
owner id: 2578
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: unmodified Lutz-Bujard LacO promoter.
mutable notes: Cleaned
mutable provenance: Lac O
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: LacO
sequence: https://synbiohub.org/public/igem/BBa_K086017_sequence/1

LacO
type: SequenceAnnotation
persistent identity: annotation1995751
display id: annotation1995751
version: 1
top level: BBa_K086017
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K086017/annotation1995751/range1995751/1

https://synbiohub.org/public/igem/BBa_K086017/annotation1995751/range1995751/1
type: Range
persistent identity: range1995751
display id: range1995751
version: 1
direction: direction/forward
top level: BBa_K086017
start: 1
end: 55
orientation: inline

BBa_M13108
type: ComponentDefinition
persistent identity: BBa M13108
display id: BBa_M13108
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: M13K07 gene VIII promoter
creator: Natalie Kuldell
created: 2006-12-15T12:00:00Z
modified: 2015-05-08T01:13:55Z
discontinued: false
dominant: true
group u list: _45_
m user id: 0
owner id: 314
owning group id: 1
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This part is from the bacteriophage M13 genome, bp 1155-1201 in M13K07. It directs transcription of M13 gene VIII (BBa_M13508, BBa_M13008). Its identity as a promoter was described in Gene (1980) 11:129-148 based on -10,-35 homology and fragment of genome able to bind RNAP.
mutable notes: none
mutable provenance: New England Biolabs M13K07. Sequenced in 2002 by L. Panganaban.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_M13108_sequence/1

BBa_K1824896
type: ComponentDefinition
persistent identity: BBa K1824896
display id: BBa_K1824896
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: J23100 + RBS
creator: Xinhao Wang
created: 2015-09-10T11:00:00Z
modified: 2015-09-11T09:57:14Z
discontinued: false
dominant: false
group u list: _2250_
m user id: 26000
owner id: 26000
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Constitutive promoter J23100 plus RBS.
mutable notes: no special concern
mutable provenance: Parts J23100 through J23119 are a family of constitutive promoter parts isolated from a small combinatorial library. B0030 is a strong RBS based on Ron Weiss thesis
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0030
component: BBa_J23100
component: BBa_K1824890
component: BBa_K1824889
sequence annotation: BBa_J23100
sequence annotation: BBa_K1824889
sequence annotation: BBa_B0030
sequence annotation: BBa_K1824890
sequence: https://synbiohub.org/public/igem/BBa_K1824896_sequence/1

BBa_B0030
type: Component
persistent identity: component2452343
display id: component2452343
version: 1
top level: BBa_K1824896
access: public
definition: https://synbiohub.org/public/igem/BBa_B0030/1

BBa_J23100
type: Component
persistent identity: component2452340
display id: component2452340
version: 1
top level: BBa_K1824896
access: public
definition: BBa_J23100

BBa_K1824890
type: Component
persistent identity: component2452345
display id: component2452345
version: 1
top level: BBa_K1824896
access: public
definition: https://synbiohub.org/public/igem/BBa_K1824890/1

BBa_K1824889
type: Component
persistent identity: component2452341
display id: component2452341
version: 1
top level: BBa_K1824896
access: public
definition: https://synbiohub.org/public/igem/BBa_K1824889/1

BBa_J23100
type: SequenceAnnotation
persistent identity: annotation2452340
display id: annotation2452340
version: 1
top level: BBa_K1824896
location: https://synbiohub.org/public/igem/BBa_K1824896/annotation2452340/range2452340/1
component: BBa_J23100

https://synbiohub.org/public/igem/BBa_K1824896/annotation2452340/range2452340/1
type: Range
persistent identity: range2452340
display id: range2452340
version: 1
direction: direction/reverse
top level: BBa_K1824896
start: 1
end: 35
orientation: inline

BBa_K1824889
type: SequenceAnnotation
persistent identity: annotation2452341
display id: annotation2452341
version: 1
top level: BBa_K1824896
location: https://synbiohub.org/public/igem/BBa_K1824896/annotation2452341/range2452341/1
component: BBa_K1824889

https://synbiohub.org/public/igem/BBa_K1824896/annotation2452341/range2452341/1
type: Range
persistent identity: range2452341
display id: range2452341
version: 1
direction: direction/reverse
top level: BBa_K1824896
start: 44
end: 51
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation2452343
display id: annotation2452343
version: 1
top level: BBa_K1824896
location: https://synbiohub.org/public/igem/BBa_K1824896/annotation2452343/range2452343/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_K1824896/annotation2452343/range2452343/1
type: Range
persistent identity: range2452343
display id: range2452343
version: 1
direction: direction/forward
top level: BBa_K1824896
start: 60
end: 74
orientation: inline

BBa_K1824890
type: SequenceAnnotation
persistent identity: annotation2452345
display id: annotation2452345
version: 1
top level: BBa_K1824896
location: https://synbiohub.org/public/igem/BBa_K1824896/annotation2452345/range2452345/1
component: BBa_K1824890

https://synbiohub.org/public/igem/BBa_K1824896/annotation2452345/range2452345/1
type: Range
persistent identity: range2452345
display id: range2452345
version: 1
direction: direction/reverse
top level: BBa_K1824896
start: 83
end: 88
orientation: inline

BBa_R2108
type: ComponentDefinition
persistent identity: BBa R2108
display id: BBa_R2108
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter with operator site for C2003
creator: Reshma Shetty
created: 2006-10-20T11:00:00Z
modified: 2015-05-08T01:14:16Z
discontinued: false
dominant: false
group u list: _41_
m user id: 0
owner id: 126
owning group id: 70
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This promoter is designed to bind to C2003.
mutable notes: Placement of the operator sites was optimized for steric interference between the C2003 and RNA polymerase based on structural models.
mutable provenance: This promoter was rationally designed.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: operator site for C2003
sequence annotation: transcription start site
sequence annotation: -35 site
sequence annotation: -10 site
sequence: https://synbiohub.org/public/igem/BBa_R2108_sequence/1

operator site for C2003
type: SequenceAnnotation
persistent identity: annotation1903909
display id: annotation1903909
version: 1
top level: BBa_R2108
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_R2108/annotation1903909/range1903909/1

https://synbiohub.org/public/igem/BBa_R2108/annotation1903909/range1903909/1
type: Range
persistent identity: range1903909
display id: range1903909
version: 1
direction: direction/forward
top level: BBa_R2108
start: 32
end: 43
orientation: inline

transcription start site
type: SequenceAnnotation
persistent identity: annotation1903911
display id: annotation1903911
version: 1
top level: BBa_R2108
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_R2108/annotation1903911/range1903911/1

https://synbiohub.org/public/igem/BBa_R2108/annotation1903911/range1903911/1
type: Range
persistent identity: range1903911
display id: range1903911
version: 1
direction: direction/forward
top level: BBa_R2108
start: 53
end: 53
orientation: inline

-35 site
type: SequenceAnnotation
persistent identity: annotation1903908
display id: annotation1903908
version: 1
top level: BBa_R2108
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R2108/annotation1903908/range1903908/1

https://synbiohub.org/public/igem/BBa_R2108/annotation1903908/range1903908/1
type: Range
persistent identity: range1903908
display id: range1903908
version: 1
direction: direction/forward
top level: BBa_R2108
start: 17
end: 22
orientation: inline

-10 site
type: SequenceAnnotation
persistent identity: annotation1903910
display id: annotation1903910
version: 1
top level: BBa_R2108
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R2108/annotation1903910/range1903910/1

https://synbiohub.org/public/igem/BBa_R2108/annotation1903910/range1903910/1
type: Range
persistent identity: range1903910
display id: range1903910
version: 1
direction: direction/forward
top level: BBa_R2108
start: 40
end: 45
orientation: inline

cI 434
type: ComponentDefinition
persistent identity: BBa R0052
display id: BBa_R0052
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter (434 cI regulated)
creator: Maia Mahoney
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: The 434 cI regulatory region sequence is a 89 base-pair sequence with the standard BioBrick prefix and suffix sections on its ends. 434 cI repressor protein, <bb_part>BBa_C0052<bb_part>, binds to it. This segment contains O-R1, O-R2, P-R, and P-RM -35.
mutable provenance: Bacteriophage 434 right operator
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BBa_R0052
sequence annotation: -10
sequence annotation: -35
sequence annotation: OR2
sequence annotation: OR1
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_R0052_sequence/1

BBa_R0052
type: SequenceAnnotation
persistent identity: annotation7068
display id: annotation7068
version: 1
top level: cI 434
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_R0052/annotation7068/range7068/1

https://synbiohub.org/public/igem/BBa_R0052/annotation7068/range7068/1
type: Range
persistent identity: range7068
display id: range7068
version: 1
direction: direction/forward
top level: cI 434
start: 1
end: 46
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2030
display id: annotation2030
version: 1
top level: cI 434
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0052/annotation2030/range2030/1

https://synbiohub.org/public/igem/BBa_R0052/annotation2030/range2030/1
type: Range
persistent identity: range2030
display id: range2030
version: 1
direction: direction/forward
top level: cI 434
start: 24
end: 29
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2031
display id: annotation2031
version: 1
top level: cI 434
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0052/annotation2031/range2031/1

https://synbiohub.org/public/igem/BBa_R0052/annotation2031/range2031/1
type: Range
persistent identity: range2031
display id: range2031
version: 1
direction: direction/forward
top level: cI 434
start: 2
end: 7
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2029
display id: annotation2029
version: 1
top level: cI 434
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0052/annotation2029/range2029/1

https://synbiohub.org/public/igem/BBa_R0052/annotation2029/range2029/1
type: Range
persistent identity: range2029
display id: range2029
version: 1
direction: direction/forward
top level: cI 434
start: 1
end: 6
orientation: inline

OR2
type: SequenceAnnotation
persistent identity: annotation2026
display id: annotation2026
version: 1
top level: cI 434
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0052/annotation2026/range2026/1

https://synbiohub.org/public/igem/BBa_R0052/annotation2026/range2026/1
type: Range
persistent identity: range2026
display id: range2026
version: 1
direction: direction/forward
top level: cI 434
start: 1
end: 43
orientation: inline

OR1
type: SequenceAnnotation
persistent identity: annotation2028
display id: annotation2028
version: 1
top level: cI 434
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0052/annotation2028/range2028/1

https://synbiohub.org/public/igem/BBa_R0052/annotation2028/range2028/1
type: Range
persistent identity: range2028
display id: range2028
version: 1
direction: direction/forward
top level: cI 434
start: 30
end: 43
orientation: inline

OR2
type: SequenceAnnotation
persistent identity: annotation2027
display id: annotation2027
version: 1
top level: cI 434
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0052/annotation2027/range2027/1

https://synbiohub.org/public/igem/BBa_R0052/annotation2027/range2027/1
type: Range
persistent identity: range2027
display id: range2027
version: 1
direction: direction/forward
top level: cI 434
start: 8
end: 21
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2032
display id: annotation2032
version: 1
top level: cI 434
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_R0052/annotation2032/range2032/1

https://synbiohub.org/public/igem/BBa_R0052/annotation2032/range2032/1
type: Range
persistent identity: range2032
display id: range2032
version: 1
direction: direction/forward
top level: cI 434
start: 35
end: 37
orientation: inline

BBa_K137088
type: ComponentDefinition
persistent identity: BBa K137088
display id: BBa_K137088
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: optimized (TA) repeat constitutive promoter with 19 bp between -10 and -35 elements
creator: Allen Lin
created: 2008-08-20T11:00:00Z
modified: 2015-05-08T01:10:10Z
discontinued: false
dominant: false
experience: experience/None
group u list: _187_
m user id: 0
owner id: 3112
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: optimized (TA) repeat constitutive promoter with 19 bp between -10 and -35 elements
mutable notes: None
mutable provenance: PCR
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K137088_sequence/1

BBa_K1330002
type: ComponentDefinition
persistent identity: BBa K1330002
display id: BBa_K1330002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Constitutive promoter (J23105)
creator: Kristian Jensen
created: 2014-09-29T11:00:00Z
modified: 2015-05-08T01:09:55Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1705_
m user id: 0
owner id: 20126
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: t
mutable notes: t
mutable provenance: t
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K1330002_sequence/1

BBa_K1682002
type: ComponentDefinition
persistent identity: BBa K1682002
display id: BBa_K1682002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PkdpF[-15, T>A]
creator: iGEM15_HKUST-Rice
created: 2015-08-09T11:00:00Z
modified: 2015-09-18T09:15:41Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2100_
m user id: 8758
owner id: 21235
owning group id: 9
status: status/Planning
bookmark: false
mutable description: kdpFp[-15, T>A]
mutable notes: kdpFp[-15, T>A]
mutable provenance: kdpFp[-15, T>A]
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: EcoRI Illegal site removed T->A
sequence annotation: -35
sequence annotation: -10
sequence annotation: phospho-KdpE binding site
sequence: https://synbiohub.org/public/igem/BBa_K1682002_sequence/1

EcoRI Illegal site removed T->A
type: SequenceAnnotation
persistent identity: annotation2475205
display id: annotation2475205
version: 1
top level: BBa_K1682002
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K1682002/annotation2475205/range2475205/1

https://synbiohub.org/public/igem/BBa_K1682002/annotation2475205/range2475205/1
type: Range
persistent identity: range2475205
display id: range2475205
version: 1
direction: direction/forward
top level: BBa_K1682002
start: 65
end: 66
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2473587
display id: annotation2473587
version: 1
top level: BBa_K1682002
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1682002/annotation2473587/range2473587/1

https://synbiohub.org/public/igem/BBa_K1682002/annotation2473587/range2473587/1
type: Range
persistent identity: range2473587
display id: range2473587
version: 1
direction: direction/forward
top level: BBa_K1682002
start: 42
end: 47
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2473586
display id: annotation2473586
version: 1
top level: BBa_K1682002
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1682002/annotation2473586/range2473586/1

https://synbiohub.org/public/igem/BBa_K1682002/annotation2473586/range2473586/1
type: Range
persistent identity: range2473586
display id: range2473586
version: 1
direction: direction/forward
top level: BBa_K1682002
start: 69
end: 74
orientation: inline

phospho-KdpE binding site
type: SequenceAnnotation
persistent identity: annotation2473585
display id: annotation2473585
version: 1
top level: BBa_K1682002
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1682002/annotation2473585/range2473585/1

https://synbiohub.org/public/igem/BBa_K1682002/annotation2473585/range2473585/1
type: Range
persistent identity: range2473585
display id: range2473585
version: 1
direction: direction/forward
top level: BBa_K1682002
start: 8
end: 24
orientation: inline

BBa_K1660007
type: ComponentDefinition
persistent identity: BBa K1660007
display id: BBa_K1660007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PompR-6N-gp35-Terminator
creator: Dai Yuanyi
created: 2015-09-07T11:00:00Z
modified: 2015-09-08T12:46:10Z
discontinued: false
dominant: false
group u list: _2078_
m user id: 26933
owner id: 26933
owning group id: 9
status: status/Planning
bookmark: false
mutable description: PompR is a OmpR-controlled promoter which can be positively regulated by phosphorylated OmpR. This promoter is taken from the upstream region of ompC. Phosphorylated OmpR binds to the three operator sites and activates transcription. We insert PompR(BBa_R0082) sequences into the upstream region of our target gene gp35 through restriction-ligation method, which together is connected to vector PSB1C3 afterwards, and therefore we are able to regulate the expression of in through the control of light. Bxb1 gp35 is a serine integrase in Mycobacterium phage Bxb1. This intergrase can exclusively catalyze site-specific recombination. The dismiss of light singal can drive the expression of integrase, which alone can set a DNA sequence(there it means the promoter) flanked by oppositional attB and attP sites, thereby produce an inverted sequence flanked by attL and attR sites. As a consequence, the promotor moves on to express the toxic protein instead of the attractant. We insert the gp35 gene into the downstream region of the PompR promotor by means of 3A synthesis so that the expression of integrase can be regulated by light. In addition, we add a 6N promotor between PompR and gp35 to decrease basal integrase expression above flipping threshold level which could lower the efficiency of int translation. Meanwhile, we insert the recombined sites of attB and attP into the flanking sequences of our constitutive promotor (BBa_J23110 ) and so far we accomplish our reverse promotor system.
mutable notes: we add a 6N promotor between PompR and gp35 to decrease basal integrase expression above flipping threshold level which could lower the efficiency of int translation.
mutable provenance: It comes from the register.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_R0082
component: BBa_K1433008
sequence annotation: BBa_R0082
sequence annotation: BBa_K1433008
sequence: https://synbiohub.org/public/igem/BBa_K1660007_sequence/1

BBa_R0082
type: Component
persistent identity: component2446518
display id: component2446518
version: 1
top level: BBa_K1660007
access: public
definition: OmpR

BBa_K1433008
type: Component
persistent identity: component2446523
display id: component2446523
version: 1
top level: BBa_K1660007
access: public
definition: https://synbiohub.org/public/igem/BBa_K1433008/1

BBa_R0082
type: SequenceAnnotation
persistent identity: annotation2446518
display id: annotation2446518
version: 1
top level: BBa_K1660007
location: https://synbiohub.org/public/igem/BBa_K1660007/annotation2446518/range2446518/1
component: BBa_R0082

https://synbiohub.org/public/igem/BBa_K1660007/annotation2446518/range2446518/1
type: Range
persistent identity: range2446518
display id: range2446518
version: 1
direction: direction/reverse
top level: BBa_K1660007
start: 1
end: 108
orientation: inline

BBa_K1433008
type: SequenceAnnotation
persistent identity: annotation2446523
display id: annotation2446523
version: 1
top level: BBa_K1660007
location: https://synbiohub.org/public/igem/BBa_K1660007/annotation2446523/range2446523/1
component: BBa_K1433008

https://synbiohub.org/public/igem/BBa_K1660007/annotation2446523/range2446523/1
type: Range
persistent identity: range2446523
display id: range2446523
version: 1
direction: direction/reverse
top level: BBa_K1660007
start: 117
end: 1773
orientation: inline

BBa_K823004
type: ComponentDefinition
persistent identity: BBa K823004
display id: BBa_K823004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Anderson promoter J23100
creator: Korinna Kraft
created: 2012-09-07T11:00:00Z
modified: 2015-07-07T11:43:33Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1081_
m user id: 4206
owner id: 12081
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Anderson promoter J23100 without RFP in backbone vector pSB1C3 to easily fuse the promoter with other reporters e.g. the lux operon.
mutable notes: no considerations
mutable provenance: Partsregistry
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: J23100
sequence annotation: -10 box
sequence annotation: -35 box
sequence: https://synbiohub.org/public/igem/BBa_K823004_sequence/1

J23100
type: SequenceAnnotation
persistent identity: annotation2182506
display id: annotation2182506
version: 1
top level: BBa_K823004
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K823004/annotation2182506/range2182506/1

https://synbiohub.org/public/igem/BBa_K823004/annotation2182506/range2182506/1
type: Range
persistent identity: range2182506
display id: range2182506
version: 1
direction: direction/forward
top level: BBa_K823004
start: 1
end: 35
orientation: inline

-10 box
type: SequenceAnnotation
persistent identity: annotation2182508
display id: annotation2182508
version: 1
top level: BBa_K823004
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K823004/annotation2182508/range2182508/1

https://synbiohub.org/public/igem/BBa_K823004/annotation2182508/range2182508/1
type: Range
persistent identity: range2182508
display id: range2182508
version: 1
direction: direction/forward
top level: BBa_K823004
start: 24
end: 29
orientation: inline

-35 box
type: SequenceAnnotation
persistent identity: annotation2182507
display id: annotation2182507
version: 1
top level: BBa_K823004
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K823004/annotation2182507/range2182507/1

https://synbiohub.org/public/igem/BBa_K823004/annotation2182507/range2182507/1
type: Range
persistent identity: range2182507
display id: range2182507
version: 1
direction: direction/forward
top level: BBa_K823004
start: 1
end: 6
orientation: inline

BBa_J13210
type: ComponentDefinition
persistent identity: BBa J13210
display id: BBa_J13210
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pOmpR dependent POPS producer
creator: Jeff Tabor
created: 2005-07-18T11:00:00Z
modified: 2015-08-31T04:08:30Z
discontinued: false
dominant: false
experience: experience/None
group u list: _37_
m user id: 0
owner id: 88
owning group id: 37
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
component: BBa_B0012
component: BBa_R0082
component: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_B0010
sequence annotation: BBa_R0082
sequence: https://synbiohub.org/public/igem/BBa_J13210_sequence/1

BBa_B0012
type: Component
persistent identity: component1587097
display id: component1587097
version: 1
top level: BBa_J13210
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_R0082
type: Component
persistent identity: component1587117
display id: component1587117
version: 1
top level: BBa_J13210
access: public
definition: OmpR

BBa_B0010
type: Component
persistent identity: component1587087
display id: component1587087
version: 1
top level: BBa_J13210
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1587097
display id: annotation1587097
version: 1
top level: BBa_J13210
location: https://synbiohub.org/public/igem/BBa_J13210/annotation1587097/range1587097/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_J13210/annotation1587097/range1587097/1
type: Range
persistent identity: range1587097
display id: range1587097
version: 1
direction: direction/forward
top level: BBa_J13210
start: 89
end: 129
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1587087
display id: annotation1587087
version: 1
top level: BBa_J13210
location: https://synbiohub.org/public/igem/BBa_J13210/annotation1587087/range1587087/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_J13210/annotation1587087/range1587087/1
type: Range
persistent identity: range1587087
display id: range1587087
version: 1
direction: direction/forward
top level: BBa_J13210
start: 1
end: 80
orientation: inline

BBa_R0082
type: SequenceAnnotation
persistent identity: annotation1587117
display id: annotation1587117
version: 1
top level: BBa_J13210
location: https://synbiohub.org/public/igem/BBa_J13210/annotation1587117/range1587117/1
component: BBa_R0082

https://synbiohub.org/public/igem/BBa_J13210/annotation1587117/range1587117/1
type: Range
persistent identity: range1587117
display id: range1587117
version: 1
direction: direction/forward
top level: BBa_J13210
start: 138
end: 245
orientation: inline

BBa_J23118
type: ComponentDefinition
persistent identity: BBa J23118
display id: BBa_J23118
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: constitutive promoter family member
creator: John Anderson
created: 2006-08-16T11:00:00Z
modified: 2015-08-31T04:08:40Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: Later
mutable notes: N/A
mutable provenance: Later
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J23118_sequence/1

BBa_I12040
type: ComponentDefinition
persistent identity: BBa I12040
display id: BBa_I12040
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Modified lambda P(RM) promoter: -10 region from P(L) and cooperatively repressed by 434 cI
creator: ryhsiao
created: 2004-08-18T11:00:00Z
modified: 2015-08-31T04:07:31Z
discontinued: false
dominant: false
experience: experience/None
group u list: _3_
m user id: 0
owner id: 148
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Lamdba P(RM) promoter modified to be activated by lamda repressor (cI) and repressed by 434 repressor (cI). Two operator sites are used for both activation and repression to enhance cooperativity. Additionally, the -10 region from lambda P(L) was used instead of that from P(RM).
mutable notes: The O-R3 site of lambda (17 nt) was replaced by the O-R1 site of 434 (14 nt) to facilitate repression by 434 cI. The new 434 O-R1 is 5'-justified with the lambda O-R3 it replaced, leaving the -10 sequence intact. The 434 O-R2 site replaces the wildtype labmda promoter starting at -5, thereby preserving the 8 nt between 434 O-R1 and O-R2 in the wildtype. The mRNA transcript will contain an additional 9 nt compared to wildtype lambda phage. This part differs from BBa_I12036 because the -10 region from P(L) was used instead of that from P(RM).
mutable provenance: Bushman, F. D. The bacteriophage 434 right operator roles of O-R1, O-R2, and O-R3. J. Mol. Biol. (1993) 230, 28-40. ; Lutz, R & Bujard, H. "Independent and tight regulation of transcriptional units in Escherichia coli via the lacR/O, the TetR/O and AraC/I1-I2 regulatory elements, Nucleic Acids Research, 1997, Vol.25, No.6 (1203-1210)
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -10
sequence annotation: OR1 lambda
sequence annotation: OR2 434
sequence annotation: OR2 lambda
sequence annotation: -35
sequence annotation: OR1 434
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_I12040_sequence/1

-10
type: SequenceAnnotation
persistent identity: annotation1059302
display id: annotation1059302
version: 1
top level: BBa_I12040
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I12040/annotation1059302/range1059302/1

https://synbiohub.org/public/igem/BBa_I12040/annotation1059302/range1059302/1
type: Range
persistent identity: range1059302
display id: range1059302
version: 1
direction: direction/forward
top level: BBa_I12040
start: 71
end: 76
orientation: inline

OR1 lambda
type: SequenceAnnotation
persistent identity: annotation1059298
display id: annotation1059298
version: 1
top level: BBa_I12040
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I12040/annotation1059298/range1059298/1

https://synbiohub.org/public/igem/BBa_I12040/annotation1059298/range1059298/1
type: Range
persistent identity: range1059298
display id: range1059298
version: 1
direction: direction/forward
top level: BBa_I12040
start: 9
end: 25
orientation: inline

OR2 434
type: SequenceAnnotation
persistent identity: annotation1059303
display id: annotation1059303
version: 1
top level: BBa_I12040
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I12040/annotation1059303/range1059303/1

https://synbiohub.org/public/igem/BBa_I12040/annotation1059303/range1059303/1
type: Range
persistent identity: range1059303
display id: range1059303
version: 1
direction: direction/forward
top level: BBa_I12040
start: 78
end: 91
orientation: inline

OR2 lambda
type: SequenceAnnotation
persistent identity: annotation1059299
display id: annotation1059299
version: 1
top level: BBa_I12040
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I12040/annotation1059299/range1059299/1

https://synbiohub.org/public/igem/BBa_I12040/annotation1059299/range1059299/1
type: Range
persistent identity: range1059299
display id: range1059299
version: 1
direction: direction/forward
top level: BBa_I12040
start: 33
end: 49
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1059300
display id: annotation1059300
version: 1
top level: BBa_I12040
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I12040/annotation1059300/range1059300/1

https://synbiohub.org/public/igem/BBa_I12040/annotation1059300/range1059300/1
type: Range
persistent identity: range1059300
display id: range1059300
version: 1
direction: direction/forward
top level: BBa_I12040
start: 48
end: 53
orientation: inline

OR1 434
type: SequenceAnnotation
persistent identity: annotation1059301
display id: annotation1059301
version: 1
top level: BBa_I12040
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I12040/annotation1059301/range1059301/1

https://synbiohub.org/public/igem/BBa_I12040/annotation1059301/range1059301/1
type: Range
persistent identity: range1059301
display id: range1059301
version: 1
direction: direction/forward
top level: BBa_I12040
start: 56
end: 69
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation1062263
display id: annotation1062263
version: 1
top level: BBa_I12040
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_I12040/annotation1062263/range1062263/1

https://synbiohub.org/public/igem/BBa_I12040/annotation1062263/range1062263/1
type: Range
persistent identity: range1062263
display id: range1062263
version: 1
direction: direction/forward
top level: BBa_I12040
start: 83
end: 83
orientation: inline

BBa_K180005
type: ComponentDefinition
persistent identity: BBa K180005
display id: BBa_K180005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: GoL - Primary plasmid (part 1)/RPS - Paper primary plasmid (part 1) [LuxR generator]
creator: Richard Wilson, Stephanie Chambers
created: 2009-10-15T11:00:00Z
modified: 2015-05-08T01:11:06Z
discontinued: false
dominant: false
experience: experience/None
group u list: _278_
m user id: 0
owner id: 4082
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Part of the iGEM2009 Team:Southampton Game of Life primary plasmid.
mutable notes: Constitutive promoter selected to allow for continuous expression of C0062.
mutable provenance: See relevant basic biobrick parts.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_J23119
component: BBa_C0062
component: BBa_B0015
component: BBa_B0034
sequence annotation: BBa_B0034
sequence annotation: BBa_C0062
sequence annotation: BBa_J23119
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_K180005_sequence/1

BBa_J23119
type: Component
persistent identity: component2255824
display id: component2255824
version: 1
top level: BBa_K180005
access: public
definition: BBa_J23119

BBa_C0062
type: Component
persistent identity: component2255829
display id: component2255829
version: 1
top level: BBa_K180005
access: public
definition: https://synbiohub.org/public/igem/BBa_C0062/1

BBa_B0015
type: Component
persistent identity: component2255839
display id: component2255839
version: 1
top level: BBa_K180005
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_B0034
type: Component
persistent identity: component2255826
display id: component2255826
version: 1
top level: BBa_K180005
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2255826
display id: annotation2255826
version: 1
top level: BBa_K180005
location: https://synbiohub.org/public/igem/BBa_K180005/annotation2255826/range2255826/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K180005/annotation2255826/range2255826/1
type: Range
persistent identity: range2255826
display id: range2255826
version: 1
direction: direction/reverse
top level: BBa_K180005
start: 44
end: 55
orientation: inline

BBa_C0062
type: SequenceAnnotation
persistent identity: annotation2255829
display id: annotation2255829
version: 1
top level: BBa_K180005
location: https://synbiohub.org/public/igem/BBa_K180005/annotation2255829/range2255829/1
component: BBa_C0062

https://synbiohub.org/public/igem/BBa_K180005/annotation2255829/range2255829/1
type: Range
persistent identity: range2255829
display id: range2255829
version: 1
direction: direction/forward
top level: BBa_K180005
start: 62
end: 817
orientation: inline

BBa_J23119
type: SequenceAnnotation
persistent identity: annotation2255824
display id: annotation2255824
version: 1
top level: BBa_K180005
location: https://synbiohub.org/public/igem/BBa_K180005/annotation2255824/range2255824/1
component: BBa_J23119

https://synbiohub.org/public/igem/BBa_K180005/annotation2255824/range2255824/1
type: Range
persistent identity: range2255824
display id: range2255824
version: 1
direction: direction/reverse
top level: BBa_K180005
start: 1
end: 35
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2255839
display id: annotation2255839
version: 1
top level: BBa_K180005
location: https://synbiohub.org/public/igem/BBa_K180005/annotation2255839/range2255839/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K180005/annotation2255839/range2255839/1
type: Range
persistent identity: range2255839
display id: range2255839
version: 1
direction: direction/reverse
top level: BBa_K180005
start: 851
end: 979
orientation: inline

BBa_I12036
type: ComponentDefinition
persistent identity: BBa I12036
display id: BBa_I12036
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Modified lamdba Prm promoter (cooperative repression by 434 cI)
creator: Hans
created: 2004-08-17T11:00:00Z
modified: 2015-08-31T04:07:31Z
discontinued: false
dominant: false
experience: experience/None
group u list: _3_
m user id: 0
owner id: 103
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Lamdba Prm promoter modified to be activated by lamda repressor (cI) and repressed by 434 repressor (cI). Two operators are used for both activation and repression to enhance cooperativity.
mutable notes: The O-R3 site of lambda (17 nt) was replaced by the O-R1 site of 434 (14 nt) to facilitate repression by 434 cI. The new 434 O-R1 is 5'-justified with the lambda O-R3 it replaced, leaving the -10 sequence intact. The 434 O-R2 site replaces the wildtype labmda promoter starting at -5, thereby preserving the 8 nt between 434 O-R1 and O-R2 in the wildtype. The mRNA transcript will contain an additional 9 nt compared to wildtype lambda phage.
mutable provenance: Bushman, F. D. The bacteriophage 434 right operator roles of O-R1, O-R2, and O-R3. J. Mol. Biol. (1993) 230, 28-40.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: OR2 434
sequence annotation: -35
sequence annotation: -10
sequence annotation: OR2 lambda
sequence annotation: OR1 lambda
sequence annotation: OR1 434
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_I12036_sequence/1

OR2 434
type: SequenceAnnotation
persistent identity: annotation1056738
display id: annotation1056738
version: 1
top level: BBa_I12036
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I12036/annotation1056738/range1056738/1

https://synbiohub.org/public/igem/BBa_I12036/annotation1056738/range1056738/1
type: Range
persistent identity: range1056738
display id: range1056738
version: 1
direction: direction/forward
top level: BBa_I12036
start: 78
end: 91
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1056739
display id: annotation1056739
version: 1
top level: BBa_I12036
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I12036/annotation1056739/range1056739/1

https://synbiohub.org/public/igem/BBa_I12036/annotation1056739/range1056739/1
type: Range
persistent identity: range1056739
display id: range1056739
version: 1
direction: direction/forward
top level: BBa_I12036
start: 48
end: 53
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1056740
display id: annotation1056740
version: 1
top level: BBa_I12036
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I12036/annotation1056740/range1056740/1

https://synbiohub.org/public/igem/BBa_I12036/annotation1056740/range1056740/1
type: Range
persistent identity: range1056740
display id: range1056740
version: 1
direction: direction/forward
top level: BBa_I12036
start: 71
end: 76
orientation: inline

OR2 lambda
type: SequenceAnnotation
persistent identity: annotation1056736
display id: annotation1056736
version: 1
top level: BBa_I12036
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I12036/annotation1056736/range1056736/1

https://synbiohub.org/public/igem/BBa_I12036/annotation1056736/range1056736/1
type: Range
persistent identity: range1056736
display id: range1056736
version: 1
direction: direction/forward
top level: BBa_I12036
start: 33
end: 49
orientation: inline

OR1 lambda
type: SequenceAnnotation
persistent identity: annotation1056735
display id: annotation1056735
version: 1
top level: BBa_I12036
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I12036/annotation1056735/range1056735/1

https://synbiohub.org/public/igem/BBa_I12036/annotation1056735/range1056735/1
type: Range
persistent identity: range1056735
display id: range1056735
version: 1
direction: direction/forward
top level: BBa_I12036
start: 9
end: 25
orientation: inline

OR1 434
type: SequenceAnnotation
persistent identity: annotation1056737
display id: annotation1056737
version: 1
top level: BBa_I12036
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I12036/annotation1056737/range1056737/1

https://synbiohub.org/public/igem/BBa_I12036/annotation1056737/range1056737/1
type: Range
persistent identity: range1056737
display id: range1056737
version: 1
direction: direction/forward
top level: BBa_I12036
start: 56
end: 69
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation1062203
display id: annotation1062203
version: 1
top level: BBa_I12036
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_I12036/annotation1062203/range1062203/1

https://synbiohub.org/public/igem/BBa_I12036/annotation1062203/range1062203/1
type: Range
persistent identity: range1062203
display id: range1062203
version: 1
direction: direction/forward
top level: BBa_I12036
start: 83
end: 83
orientation: inline

BBa_K1585100
type: ComponentDefinition
persistent identity: BBa K1585100
display id: BBa_K1585100
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Anderson Promoter with lacI binding site
creator: Tobias Schwanemann
created: 2015-08-23T11:00:00Z
modified: 2015-08-26T11:28:17Z
discontinued: false
dominant: false
experience: experience/Fails
group u list: _2002_
m user id: 24474
owner id: 24474
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Promoters of the Anderson Promoter Collection with lacI binding site derived from [http://parts.igem.org/Part:BBa_R0010 R_0010] to make them [http://openwetware.org/wiki/IPTG IPTG] iducible.
mutable notes: The "lacI binding site" of Part [http://parts.igem.org/Part:BBa_R0010 R_0010] differs from lacO from Part [http://parts.igem.org/Part:BBa_J33207 BBa_J33207] although they should be same. We choosed part BBa_R0010 because we assumed older parts would be reliable.
mutable provenance: Assambly of [http://parts.igem.org/Part:BBa_J23100 J23100] and lacI-binding-site of [http://parts.igem.org/Part:BBa_R0010 R_0010]
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
component: BBa_K1585999
component: BBa_J23100
sequence annotation: BBa_K1585999
sequence annotation: BBa_J23100
sequence: https://synbiohub.org/public/igem/BBa_K1585100_sequence/1

BBa_K1585999
type: Component
persistent identity: component2436913
display id: component2436913
version: 1
top level: BBa_K1585100
access: public
definition: https://synbiohub.org/public/igem/BBa_K1585999/1

BBa_J23100
type: Component
persistent identity: component2436912
display id: component2436912
version: 1
top level: BBa_K1585100
access: public
definition: BBa_J23100

BBa_K1585999
type: SequenceAnnotation
persistent identity: annotation2436913
display id: annotation2436913
version: 1
top level: BBa_K1585100
location: https://synbiohub.org/public/igem/BBa_K1585100/annotation2436913/range2436913/1
component: BBa_K1585999

https://synbiohub.org/public/igem/BBa_K1585100/annotation2436913/range2436913/1
type: Range
persistent identity: range2436913
display id: range2436913
version: 1
direction: direction/reverse
top level: BBa_K1585100
start: 44
end: 78
orientation: inline

BBa_J23100
type: SequenceAnnotation
persistent identity: annotation2436912
display id: annotation2436912
version: 1
top level: BBa_K1585100
location: https://synbiohub.org/public/igem/BBa_K1585100/annotation2436912/range2436912/1
component: BBa_J23100

https://synbiohub.org/public/igem/BBa_K1585100/annotation2436912/range2436912/1
type: Range
persistent identity: range2436912
display id: range2436912
version: 1
direction: direction/reverse
top level: BBa_K1585100
start: 1
end: 35
orientation: inline

BBa_J23112
type: ComponentDefinition
persistent identity: BBa J23112
display id: BBa_J23112
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: constitutive promoter family member
creator: John Anderson
created: 2006-08-16T11:00:00Z
modified: 2015-08-31T04:08:40Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: Later
mutable notes: N/A
mutable provenance: Later
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J23112_sequence/1

BBa_K112402
type: ComponentDefinition
persistent identity: BBa K112402
display id: BBa_K112402
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: promoter for FabA gene - Membrane Damage and Ultrasound Senstitive
creator: Christina Brown
created: 2008-10-20T11:00:00Z
modified: 2015-05-08T01:09:17Z
discontinued: false
dominant: false
group u list: _224_
m user id: 0
owner id: 3241
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: According to Vollmer, et. al in their 1998 Applied and Enviormental Microbiology paper, "Bacterial Stress Responses to 1-Megahertz Pulsed Ultrasound in the Presence of Microbubble", the promoter for the fabA gene, a known membrane damage response gene, is upregulated seven fold when cells are exposed to high frequency ultrasound and microbubble cavitation.
mutable notes: none
mutable provenance: cloned from E. coli MG1655 genome
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K112402_sequence/1

BBa_K137091
type: ComponentDefinition
persistent identity: BBa K137091
display id: BBa_K137091
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: optimized (A) repeat constitutive promoter with 18 bp between -10 and -35 elements
creator: Allen Lin
created: 2008-08-20T11:00:00Z
modified: 2015-05-08T01:10:10Z
discontinued: false
dominant: false
experience: experience/None
group u list: _187_
m user id: 0
owner id: 3112
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: optimized (A) repeat constitutive promoter with 18 bp between -10 and -35 elements
mutable notes: None
mutable provenance: PCR
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K137091_sequence/1

BBa_J34809
type: ComponentDefinition
persistent identity: BBa J34809
display id: BBa_J34809
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: promoter lac induced
creator: Alexandra Choutko
created: 2006-10-25T11:00:00Z
modified: 2015-08-31T04:08:47Z
discontinued: false
dominant: false
group u list: _62_
m user id: 0
owner id: 1085
owning group id: 62
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: improved for transcription factor BBa_J34807 binding
mutable notes: a
mutable provenance: a
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J34809_sequence/1

BBa_J54200
type: ComponentDefinition
persistent identity: BBa J54200
display id: BBa_J54200
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: lacq_Promoter
creator: Yusaku Nakashima
created: 2006-10-14T11:00:00Z
modified: 2015-08-31T03:48:12Z
discontinued: false
dominant: false
group u list: _76_
m user id: 0
owner id: 762
owning group id: 76
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Promoter that express always.
mutable notes: As promoter
mutable provenance: DNA synthesis
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BamHI
sequence annotation: lacq Promoter
sequence: https://synbiohub.org/public/igem/BBa_J54200_sequence/1

BamHI
type: SequenceAnnotation
persistent identity: annotation1903214
display id: annotation1903214
version: 1
top level: BBa_J54200
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_J54200/annotation1903214/range1903214/1

https://synbiohub.org/public/igem/BBa_J54200/annotation1903214/range1903214/1
type: Range
persistent identity: range1903214
display id: range1903214
version: 1
direction: direction/forward
top level: BBa_J54200
start: 7
end: 13
orientation: inline

lacq Promoter
type: SequenceAnnotation
persistent identity: annotation1903215
display id: annotation1903215
version: 1
top level: BBa_J54200
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J54200/annotation1903215/range1903215/1

https://synbiohub.org/public/igem/BBa_J54200/annotation1903215/range1903215/1
type: Range
persistent identity: range1903215
display id: range1903215
version: 1
direction: direction/forward
top level: BBa_J54200
start: 14
end: 48
orientation: inline

P1
type: ComponentDefinition
persistent identity: BBa K100001
display id: BBa_K100001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Edited Xylose Regulated Bi-Directional Operator 1
creator: Garrett Tobin
created: 2008-08-17T11:00:00Z
modified: 2015-05-08T01:08:41Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _213_
m user id: 0
owner id: 1752
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part contains the operator region controlling transcription for the xyl operon. This regulatory region is dependent upon the binding of CRP-cAMP and XylR bound to xylose for activation. Upon binding, transcription is activated in both directions (xylFGH and xylAB facing). This part has a scrambled CRP binding site and and altered RNA ploymerase binding site that was designed to more resemble the consensus sequence slightly.
mutable notes: Both directions were kept in the sequence of the part even though it is intended to work in the right facing direction because of possible looping that may occur as a transcriptional control. The RNA polymerase binding site was altered to make binding stronger, attempting to allow activation when XylR-xylose alone is bound (the absence of CRP-cAMP).
mutable provenance: Taken from E. Coli genome bases 3728830-3729092, base pairs changed at RNA polymerase bining sites.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: Scrambled CRP Binding Site
sequence annotation: RNA poly. Binding Site
sequence: https://synbiohub.org/public/igem/BBa_K100001_sequence/1

Scrambled CRP Binding Site
type: SequenceAnnotation
persistent identity: annotation1972256
display id: annotation1972256
version: 1
top level: P1
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K100001/annotation1972256/range1972256/1

https://synbiohub.org/public/igem/BBa_K100001/annotation1972256/range1972256/1
type: Range
persistent identity: range1972256
display id: range1972256
version: 1
direction: direction/forward
top level: P1
start: 88
end: 104
orientation: inline

RNA poly. Binding Site
type: SequenceAnnotation
persistent identity: annotation1972254
display id: annotation1972254
version: 1
top level: P1
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K100001/annotation1972254/range1972254/1

https://synbiohub.org/public/igem/BBa_K100001/annotation1972254/range1972254/1
type: Range
persistent identity: range1972254
display id: range1972254
version: 1
direction: direction/reverse
top level: P1
start: 21
end: 26
orientation: inline

RNA poly. Binding Site
type: SequenceAnnotation
persistent identity: annotation1972257
display id: annotation1972257
version: 1
top level: P1
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K100001/annotation1972257/range1972257/1

https://synbiohub.org/public/igem/BBa_K100001/annotation1972257/range1972257/1
type: Range
persistent identity: range1972257
display id: range1972257
version: 1
direction: direction/forward
top level: P1
start: 243
end: 248
orientation: inline

RNA poly. Binding Site
type: SequenceAnnotation
persistent identity: annotation1972258
display id: annotation1972258
version: 1
top level: P1
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K100001/annotation1972258/range1972258/1

https://synbiohub.org/public/igem/BBa_K100001/annotation1972258/range1972258/1
type: Range
persistent identity: range1972258
display id: range1972258
version: 1
direction: direction/forward
top level: P1
start: 265
end: 270
orientation: inline

RNA poly. Binding Site
type: SequenceAnnotation
persistent identity: annotation1972255
display id: annotation1972255
version: 1
top level: P1
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K100001/annotation1972255/range1972255/1

https://synbiohub.org/public/igem/BBa_K100001/annotation1972255/range1972255/1
type: Range
persistent identity: range1972255
display id: range1972255
version: 1
direction: direction/reverse
top level: P1
start: 45
end: 50
orientation: inline

BBa_J23110
type: ComponentDefinition
persistent identity: BBa J23110
display id: BBa_J23110
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: constitutive promoter family member
creator: John Anderson
created: 2006-08-16T11:00:00Z
modified: 2015-08-31T04:08:40Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: true
mutable description: Later
mutable notes: N/A
mutable provenance: Later
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J23110_sequence/1

Ara
type: ComponentDefinition
persistent identity: BBa K808000
display id: BBa_K808000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: araC-Pbad - Arabinose inducible regulatory promoter/repressor unit
creator: Valentina Herbring, Sebastian Palluk, Andreas Schmidt
created: 2012-08-10T11:00:00Z
modified: 2015-05-08T01:13:25Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1065_
m user id: 0
owner id: 11792
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: This promoter can be induced by arabinose. In presens of arabinose the promotor is active.
mutable notes: non
mutable provenance: Guzman LM, Belin D, Carson MJ, Beckwith J. Tight regulation, modulation, and high-level expression by vectors containing the arabinose PBAD promoter. J Bacteriol. 1995 Jul;177(14):4121-30 Khlebnikov A, Skaug T, Keasling JD, modulation of gene expression from the arabinose-inducible araBAD promoter,J Ind Microbiol Biotechnol. 2002 Jul;29(1):34-7.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: AraO1
sequence annotation: AraO
sequence annotation: AraPromoter
sequence annotation: AraC
sequence: https://synbiohub.org/public/igem/BBa_K808000_sequence/1

AraO1
type: SequenceAnnotation
persistent identity: annotation2200759
display id: annotation2200759
version: 1
top level: Ara
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K808000/annotation2200759/range2200759/1

https://synbiohub.org/public/igem/BBa_K808000/annotation2200759/range2200759/1
type: Range
persistent identity: range2200759
display id: range2200759
version: 1
direction: direction/forward
top level: Ara
start: 1066
end: 1077
orientation: inline

AraO
type: SequenceAnnotation
persistent identity: annotation2200758
display id: annotation2200758
version: 1
top level: Ara
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K808000/annotation2200758/range2200758/1

https://synbiohub.org/public/igem/BBa_K808000/annotation2200758/range2200758/1
type: Range
persistent identity: range2200758
display id: range2200758
version: 1
direction: direction/forward
top level: Ara
start: 909
end: 924
orientation: inline

AraPromoter
type: SequenceAnnotation
persistent identity: annotation2200760
display id: annotation2200760
version: 1
top level: Ara
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K808000/annotation2200760/range2200760/1

https://synbiohub.org/public/igem/BBa_K808000/annotation2200760/range2200760/1
type: Range
persistent identity: range2200760
display id: range2200760
version: 1
direction: direction/forward
top level: Ara
start: 1153
end: 1181
orientation: inline

AraC
type: SequenceAnnotation
persistent identity: annotation2200757
display id: annotation2200757
version: 1
top level: Ara
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K808000/annotation2200757/range2200757/1

https://synbiohub.org/public/igem/BBa_K808000/annotation2200757/range2200757/1
type: Range
persistent identity: range2200757
display id: range2200757
version: 1
direction: direction/reverse
top level: Ara
start: 1
end: 879
orientation: inline

BBa_K135000
type: ComponentDefinition
persistent identity: BBa K135000
display id: BBa_K135000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pCpxR (CpxR responsive promoter)
creator: Oliver Purcell
created: 2008-10-19T11:00:00Z
modified: 2015-05-08T01:10:01Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _183_
m user id: 0
owner id: 2796
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This is suggested to be an adhesion responsive promoter. It contains the binding site for CpxR-P (phosphorylated CpxR). CpxR is one member of the two-component Cpx response to envelope stress. Briefly, envelope stress causes CpxA, an inner-membrane histidine Kinase to Phosphorylate CpxR, allowing it to bind to CpxR-P responsive regions and regulate downstream target genes. See 'Surface sensing and adhesion of Escherichia coli controlled by the Cpx-signaling pathway', Karen Otto and Thomas J. Silhavy, PNAS, Vol. 99., No, 4., 2287-2292. 2002.
mutable notes: None
mutable provenance: This part comes from the genome of E.coli TR235, as used in the PNAS paper of Otto and Silhavy mentioned previously. TR235 is K-12 MC4100 containing a pCpxR-LacZ fusion inserted at the (Lambda)att site, plus some other modifications (see Otto and Silhavy). The part is a portion of the CpxR responsive region used in these LacZ fusions.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: CpxR-P Binding site
sequence annotation: BBa_K135000
sequence: https://synbiohub.org/public/igem/BBa_K135000_sequence/1

CpxR-P Binding site
type: SequenceAnnotation
persistent identity: annotation1982751
display id: annotation1982751
version: 1
top level: BBa_K135000
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K135000/annotation1982751/range1982751/1

https://synbiohub.org/public/igem/BBa_K135000/annotation1982751/range1982751/1
type: Range
persistent identity: range1982751
display id: range1982751
version: 1
direction: direction/forward
top level: BBa_K135000
start: 1
end: 15
orientation: inline

BBa_K135000
type: SequenceAnnotation
persistent identity: annotation1982752
display id: annotation1982752
version: 1
top level: BBa_K135000
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K135000/annotation1982752/range1982752/1

https://synbiohub.org/public/igem/BBa_K135000/annotation1982752/range1982752/1
type: Range
persistent identity: range1982752
display id: range1982752
version: 1
direction: direction/forward
top level: BBa_K135000
start: 1
end: 55
orientation: inline

BBa_J61055
type: ComponentDefinition
persistent identity: BBa J61055
display id: BBa_J61055
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: [HIP-1fnr] Promoter
creator: John Anderson
created: 2007-02-20T12:00:00Z
modified: 2015-08-31T02:03:00Z
discontinued: false
dominant: false
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: non
mutable notes: non
mutable provenance: non
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J61055_sequence/1

BBa_K145150
type: ComponentDefinition
persistent identity: BBa K145150
display id: BBa_K145150
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Hybrid promoter: HSL-LuxR activated, P22 C2 repressed
creator: Jonas Demeulemeester
created: 2008-08-05T11:00:00Z
modified: 2015-05-08T01:10:29Z
discontinued: false
dominant: false
experience: experience/None
group u list: _257_
m user id: 0
owner id: 2970
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Hybrid promoter consisting of the Lux box which overlaps partly with -35 box. Binding sites for P22 C2 OR2 and OR1 are located between the -35 and -10 boxes and downstream of them respectively.
mutable notes: -35 box is partly composite. -10 is taken from the P22 phage PR regulatory region
mutable provenance: Synthetic
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -10
sequence annotation: Lux-box
sequence annotation: OR2
sequence annotation: OR1
sequence annotation: -35
sequence: https://synbiohub.org/public/igem/BBa_K145150_sequence/1

-10
type: SequenceAnnotation
persistent identity: annotation1971585
display id: annotation1971585
version: 1
top level: BBa_K145150
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K145150/annotation1971585/range1971585/1

https://synbiohub.org/public/igem/BBa_K145150/annotation1971585/range1971585/1
type: Range
persistent identity: range1971585
display id: range1971585
version: 1
direction: direction/forward
top level: BBa_K145150
start: 42
end: 47
orientation: inline

Lux-box
type: SequenceAnnotation
persistent identity: annotation1971582
display id: annotation1971582
version: 1
top level: BBa_K145150
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K145150/annotation1971582/range1971582/1

https://synbiohub.org/public/igem/BBa_K145150/annotation1971582/range1971582/1
type: Range
persistent identity: range1971582
display id: range1971582
version: 1
direction: direction/forward
top level: BBa_K145150
start: 1
end: 20
orientation: inline

OR2
type: SequenceAnnotation
persistent identity: annotation1971584
display id: annotation1971584
version: 1
top level: BBa_K145150
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K145150/annotation1971584/range1971584/1

https://synbiohub.org/public/igem/BBa_K145150/annotation1971584/range1971584/1
type: Range
persistent identity: range1971584
display id: range1971584
version: 1
direction: direction/forward
top level: BBa_K145150
start: 23
end: 40
orientation: inline

OR1
type: SequenceAnnotation
persistent identity: annotation1971586
display id: annotation1971586
version: 1
top level: BBa_K145150
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K145150/annotation1971586/range1971586/1

https://synbiohub.org/public/igem/BBa_K145150/annotation1971586/range1971586/1
type: Range
persistent identity: range1971586
display id: range1971586
version: 1
direction: direction/forward
top level: BBa_K145150
start: 46
end: 63
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1971583
display id: annotation1971583
version: 1
top level: BBa_K145150
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K145150/annotation1971583/range1971583/1

https://synbiohub.org/public/igem/BBa_K145150/annotation1971583/range1971583/1
type: Range
persistent identity: range1971583
display id: range1971583
version: 1
direction: direction/forward
top level: BBa_K145150
start: 20
end: 25
orientation: inline

BBa_K116500
type: ComponentDefinition
persistent identity: BBa K116500
display id: BBa_K116500
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: OmpF promoter that is activated or repressesed by OmpR according to osmolarity.
creator: Chun-Ju Yang
created: 2008-10-28T12:00:00Z
modified: 2015-05-08T01:09:33Z
discontinued: false
dominant: false
group u list: _210_
m user id: 0
owner id: 2083
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Promoter OmpF is activated by phosphorylated OmpR at low osmolarity, and is repressesed by phosphorylated OmpR at high osmolarity.In Escherichia coli, osmoregulation is mediated in part by the actions of such a two-component system consisting of EnvZ and OmpR. These proteins act to control the relative levels of the outer membrane porin genes, ompF and ompC. At low osmo larity, OmpF predominates in the outer membrane, while at high osmolarity the OmpF porin is replaced by OmpC.OmpF has a larger pore and a faster ??ow rate than OmpC. Reference: K. Mattison, R. Oropeza, N. Byers, L. J. Kenney, J Mol Biol 315, 497 (Jan 25, 2002).
mutable notes: The osmolarity range of OmpF sensing still have to be confirmed
mutable provenance: Promoter OmpF is activated by phosphorylated OmpR at low osmolarity, and is repressesed by phosphorylated OmpR at high osmolarity.In Escherichia coli, osmoregulation is mediated in part by the actions of such a two-component system consisting of EnvZ and OmpR. These proteins act to control the relative levels of the outer membrane porin genes, ompF and ompC. At low osmo larity, OmpF predominates in the outer membrane, while at high osmolarity the OmpF porin is replaced by OmpC.OmpF has a larger pore and a faster ??ow rate than OmpC.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: OmpR binding
sequence: https://synbiohub.org/public/igem/BBa_K116500_sequence/1

OmpR binding
type: SequenceAnnotation
persistent identity: annotation1994040
display id: annotation1994040
version: 1
top level: BBa_K116500
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K116500/annotation1994040/range1994040/1

https://synbiohub.org/public/igem/BBa_K116500/annotation1994040/range1994040/1
type: Range
persistent identity: range1994040
display id: range1994040
version: 1
direction: direction/forward
top level: BBa_K116500
start: 27
end: 88
orientation: inline

BBa_K266006
type: ComponentDefinition
persistent identity: BBa K266006
display id: BBa_K266006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PAI+LasR -> LasI+GFP & AI+LuxR --| LasI+GFP
creator: UNAM FCIENCIAS-CINVESTAV IRA
created: 2009-10-12T11:00:00Z
modified: 2015-05-08T01:11:43Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _363_
m user id: 0
owner id: 1955
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: BBa_K091146 promoter (PAI+LasR inducible & AI+LuxR repressible) controls the policistronic expresion of LasI enzyme and GFP, double terminator
mutable notes: None.
mutable provenance: The biobricks BBa_K266005 and BBa_E0840 where used to build this biobrick.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Signalling
role: SO:0000804
component: BBa_E0840
component: BBa_K266005
sequence annotation: BBa_K266005
sequence annotation: BBa_E0840
sequence: https://synbiohub.org/public/igem/BBa_K266006_sequence/1

BBa_E0840
type: Component
persistent identity: component2267618
display id: component2267618
version: 1
top level: BBa_K266006
access: public
definition: https://synbiohub.org/public/igem/BBa_E0840/1

BBa_K266005
type: Component
persistent identity: component2267607
display id: component2267607
version: 1
top level: BBa_K266006
access: public
definition: BBa_K266005

BBa_K266005
type: SequenceAnnotation
persistent identity: annotation2267607
display id: annotation2267607
version: 1
top level: BBa_K266006
location: https://synbiohub.org/public/igem/BBa_K266006/annotation2267607/range2267607/1
component: BBa_K266005

https://synbiohub.org/public/igem/BBa_K266006/annotation2267607/range2267607/1
type: Range
persistent identity: range2267607
display id: range2267607
version: 1
direction: direction/reverse
top level: BBa_K266006
start: 1
end: 819
orientation: inline

BBa_E0840
type: SequenceAnnotation
persistent identity: annotation2267618
display id: annotation2267618
version: 1
top level: BBa_K266006
location: https://synbiohub.org/public/igem/BBa_K266006/annotation2267618/range2267618/1
component: BBa_E0840

https://synbiohub.org/public/igem/BBa_K266006/annotation2267618/range2267618/1
type: Range
persistent identity: range2267618
display id: range2267618
version: 1
direction: direction/reverse
top level: BBa_K266006
start: 828
end: 1705
orientation: inline

cI lam
type: ComponentDefinition
persistent identity: BBa R0051
display id: BBa_R0051
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: promoter (lambda cI regulated)
creator: Vinay S Mahajan, Brian Chow, Peter Carr, Voichita Marinescu and Alexander D. Wissner-Gross
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: The cI regulated promoter is based on the pR promtoer from bacteriohage lambda. The promoter has two two DNA binding sites for lambda cI repressor <bb_part>BBa_C0051</bb_part>. cI binding results in repression of transcription. The specific sequence used here is based on the cI repressible promoter used in the Elowitz repressilator (and references therein).
mutable notes: In order to address concerns about the promoter transcribing in the reverse direction, we have removed the -35 and -10 signals responsible for the promoter activity in the reverse direction. (More details needed here! DE, 2/24/03) Incompatible with host expressing cI repressor.
mutable provenance: A synthetic oscillatory network of transcriptional regulators , Elowitz M.B. , Leibler S., Nature(403),335-38: 2000
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: OR1
sequence annotation: -10
sequence annotation: -35
sequence annotation: BBa_R0051
sequence annotation: OR2
sequence: https://synbiohub.org/public/igem/BBa_R0051_sequence/1

OR1
type: SequenceAnnotation
persistent identity: annotation2024
display id: annotation2024
version: 1
top level: cI lam
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0051/annotation2024/range2024/1

https://synbiohub.org/public/igem/BBa_R0051/annotation2024/range2024/1
type: Range
persistent identity: range2024
display id: range2024
version: 1
direction: direction/forward
top level: cI lam
start: 25
end: 41
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2022
display id: annotation2022
version: 1
top level: cI lam
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0051/annotation2022/range2022/1

https://synbiohub.org/public/igem/BBa_R0051/annotation2022/range2022/1
type: Range
persistent identity: range2022
display id: range2022
version: 1
direction: direction/forward
top level: cI lam
start: 38
end: 43
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2023
display id: annotation2023
version: 1
top level: cI lam
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0051/annotation2023/range2023/1

https://synbiohub.org/public/igem/BBa_R0051/annotation2023/range2023/1
type: Range
persistent identity: range2023
display id: range2023
version: 1
direction: direction/forward
top level: cI lam
start: 15
end: 20
orientation: inline

BBa_R0051
type: SequenceAnnotation
persistent identity: annotation7067
display id: annotation7067
version: 1
top level: cI lam
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_R0051/annotation7067/range7067/1

https://synbiohub.org/public/igem/BBa_R0051/annotation7067/range7067/1
type: Range
persistent identity: range7067
display id: range7067
version: 1
direction: direction/forward
top level: cI lam
start: 1
end: 49
orientation: inline

OR2
type: SequenceAnnotation
persistent identity: annotation2025
display id: annotation2025
version: 1
top level: cI lam
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0051/annotation2025/range2025/1

https://synbiohub.org/public/igem/BBa_R0051/annotation2025/range2025/1
type: Range
persistent identity: range2025
display id: range2025
version: 1
direction: direction/forward
top level: cI lam
start: 1
end: 17
orientation: inline

OmpR
type: ComponentDefinition
persistent identity: BBa R0084
display id: BBa_R0084
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter (OmpR, positive)
creator: Stephen Lee, Roshan Kumar, Joe Levine, Ziyan Chu (Polkadorks, IAP 2004)
created: 2004-01-27T12:00:00Z
modified: 2015-05-08T01:14:15Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Positively regulated, OmpR-controlled promoter. This promoter is taken from the upstream region of ompF. Phosphorylated OmpR binds to the three operator sites and activates transcription.
mutable notes: The promoter includes three OmpR binding sites: F1, F2, and F3. The promoter starts at -107 and goes up to the transcriptional start site at +1. A distant fourth binding site, F4, has been deleted. It is involved in negative regulation of ompF (Head, Tardy and Kenney, 1998), so its deletion is intended to make the promoter solely activating. The efficacy of this promoter versus the ompC upstream region (BBa_R0082) or the truncated ompC upstream region (BBa_R0083) is currently unknown.
mutable provenance: NC_000913 E. coli K12
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -10
sequence annotation: F1 OmpR
sequence annotation: F2 OmpR
sequence annotation: -35
sequence annotation: F3 OmpR
sequence: https://synbiohub.org/public/igem/BBa_R0084_sequence/1

-10
type: SequenceAnnotation
persistent identity: annotation301318
display id: annotation301318
version: 1
top level: OmpR
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0084/annotation301318/range301318/1

https://synbiohub.org/public/igem/BBa_R0084/annotation301318/range301318/1
type: Range
persistent identity: range301318
display id: range301318
version: 1
direction: direction/forward
top level: OmpR
start: 88
end: 93
orientation: inline

F1 OmpR
type: SequenceAnnotation
persistent identity: annotation301308
display id: annotation301308
version: 1
top level: OmpR
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0084/annotation301308/range301308/1

https://synbiohub.org/public/igem/BBa_R0084/annotation301308/range301308/1
type: Range
persistent identity: range301308
display id: range301308
version: 1
direction: direction/forward
top level: OmpR
start: 11
end: 28
orientation: inline

F2 OmpR
type: SequenceAnnotation
persistent identity: annotation301309
display id: annotation301309
version: 1
top level: OmpR
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0084/annotation301309/range301309/1

https://synbiohub.org/public/igem/BBa_R0084/annotation301309/range301309/1
type: Range
persistent identity: range301309
display id: range301309
version: 1
direction: direction/forward
top level: OmpR
start: 31
end: 48
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation301317
display id: annotation301317
version: 1
top level: OmpR
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0084/annotation301317/range301317/1

https://synbiohub.org/public/igem/BBa_R0084/annotation301317/range301317/1
type: Range
persistent identity: range301317
display id: range301317
version: 1
direction: direction/forward
top level: OmpR
start: 73
end: 78
orientation: inline

F3 OmpR
type: SequenceAnnotation
persistent identity: annotation301313
display id: annotation301313
version: 1
top level: OmpR
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0084/annotation301313/range301313/1

https://synbiohub.org/public/igem/BBa_R0084/annotation301313/range301313/1
type: Range
persistent identity: range301313
display id: range301313
version: 1
direction: direction/forward
top level: OmpR
start: 51
end: 68
orientation: inline

BBa_I731004
type: ComponentDefinition
persistent identity: BBa I731004
display id: BBa_I731004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: FecA promoter
creator: Shaunak Vankudre
created: 2007-06-25T11:00:00Z
modified: 2015-08-31T04:07:55Z
discontinued: false
dominant: false
experience: experience/None
group u list: _127_
m user id: 0
owner id: 1889
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: derived from Enz et al, 2003, J Bacteriol. 2003 April; 185(7): 2387???2391. doi: 10.1128/JB.185.7.2387-2391.2003.
mutable provenance: genomic sequence from E. Coli K12
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I731004_sequence/1

BBa_J05209
type: ComponentDefinition
persistent identity: BBa J05209
display id: BBa_J05209
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Modifed Pr Promoter
creator: Alexander Roth
created: 2005-09-14T11:00:00Z
modified: 2015-08-31T04:08:15Z
discontinued: false
dominant: false
group u list: _34_
m user id: 0
owner id: 412
owning group id: 34
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This is identical to BBa_R0051 exept that some base has been modifed to alow for additional ZFP repressors to bind
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -35
sequence annotation: ZFP binding sites
sequence annotation: -10
sequence annotation: OR2
sequence: https://synbiohub.org/public/igem/BBa_J05209_sequence/1

-35
type: SequenceAnnotation
persistent identity: annotation1689811
display id: annotation1689811
version: 1
top level: BBa_J05209
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J05209/annotation1689811/range1689811/1

https://synbiohub.org/public/igem/BBa_J05209/annotation1689811/range1689811/1
type: Range
persistent identity: range1689811
display id: range1689811
version: 1
direction: direction/forward
top level: BBa_J05209
start: 15
end: 20
orientation: inline

ZFP binding sites
type: SequenceAnnotation
persistent identity: annotation1689899
display id: annotation1689899
version: 1
top level: BBa_J05209
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_J05209/annotation1689899/range1689899/1

https://synbiohub.org/public/igem/BBa_J05209/annotation1689899/range1689899/1
type: Range
persistent identity: range1689899
display id: range1689899
version: 1
direction: direction/forward
top level: BBa_J05209
start: 18
end: 29
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1689812
display id: annotation1689812
version: 1
top level: BBa_J05209
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J05209/annotation1689812/range1689812/1

https://synbiohub.org/public/igem/BBa_J05209/annotation1689812/range1689812/1
type: Range
persistent identity: range1689812
display id: range1689812
version: 1
direction: direction/forward
top level: BBa_J05209
start: 38
end: 43
orientation: inline

OR2
type: SequenceAnnotation
persistent identity: annotation1689810
display id: annotation1689810
version: 1
top level: BBa_J05209
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_J05209/annotation1689810/range1689810/1

https://synbiohub.org/public/igem/BBa_J05209/annotation1689810/range1689810/1
type: Range
persistent identity: range1689810
display id: range1689810
version: 1
direction: direction/forward
top level: BBa_J05209
start: 25
end: 41
orientation: inline

OR2
type: SequenceAnnotation
persistent identity: annotation1689809
display id: annotation1689809
version: 1
top level: BBa_J05209
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_J05209/annotation1689809/range1689809/1

https://synbiohub.org/public/igem/BBa_J05209/annotation1689809/range1689809/1
type: Range
persistent identity: range1689809
display id: range1689809
version: 1
direction: direction/forward
top level: BBa_J05209
start: 1
end: 17
orientation: inline

BBa_K088007
type: ComponentDefinition
persistent identity: BBa K088007
display id: BBa_K088007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: GlnRS promoter
creator: Yaguang Liu
created: 2008-10-23T11:00:00Z
modified: 2015-05-08T01:08:37Z
discontinued: false
dominant: false
group u list: _190_
m user id: 0
owner id: 3108
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part promotes the transcription of TyrRS(aminoacyl tRNA synthetase).
mutable notes: It is a weak promoter.
mutable provenance: E.coli We synthetized it according to the GeneBank.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K088007_sequence/1

BBa_J23100
type: ComponentDefinition
persistent identity: BBa J23100
display id: BBa_J23100
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: constitutive promoter family member
creator: John Anderson
created: 2006-08-03T11:00:00Z
modified: 2015-08-31T04:08:40Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: Replace later
mutable notes: N/A
mutable provenance: Isolated from library of promoters
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J23100_sequence/1

KP3
type: ComponentDefinition
persistent identity: BBa J01006
display id: BBa_J01006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Key Promoter absorbs 3
creator: Golden Bear
created: 2005-10-31T12:00:00Z
modified: 2015-08-31T04:08:11Z
discontinued: false
dominant: false
group u list: _13_
m user id: 0
owner id: 395
owning group id: 13
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Promoter for transcribing keys to act on locks based on Isaacs, Collins, et. al: "Engineered riboregulators enable post-transcriptional control of gene expression" KP3 absorbs three nucleotides from mixed site ("TACTAGAG"), so that the key has a 5 nucleotide spacer region (i.e. "TAGAG") between the transcription start site and the first nucleotide of the key. We are still looking into what the optimal size for the spacer region is. See also KP2 (BBa_J01007), and Key1 (BBa_J01008), Key2 (BBa_J01009), Lock1 (BBa_J01010), and Lock2 (BBa_J01011).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J01006_sequence/1

BBa_K180016
type: ComponentDefinition
persistent identity: BBa K180016
display id: BBa_K180016
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Rock-paper-scissors - Paper secondary plasmid [lux pR, LacI generator]
creator: Richard Wilson, Stephanie Chambers
created: 2009-10-18T11:00:00Z
modified: 2015-05-08T01:11:06Z
discontinued: false
dominant: false
experience: experience/None
group u list: _278_
m user id: 0
owner id: 4082
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: iGEM2009 Team:Southampton rock-paper-scissors 'Rock' secondary plasmid.
mutable notes: Regulated expression of LacI (C0012) by Lux pR (R0062).
mutable provenance: See relevant basic biobrick parts (R0062, B0034, C0012, B0015).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_C0012
component: BBa_R0062
component: BBa_B0034
component: BBa_B0015
sequence annotation: BBa_B0034
sequence annotation: BBa_B0015
sequence annotation: BBa_C0012
sequence annotation: BBa_R0062
sequence: https://synbiohub.org/public/igem/BBa_K180016_sequence/1

BBa_C0012
type: Component
persistent identity: component2256442
display id: component2256442
version: 1
top level: BBa_K180016
access: public
definition: https://synbiohub.org/public/igem/BBa_C0012/1

BBa_R0062
type: Component
persistent identity: component2256434
display id: component2256434
version: 1
top level: BBa_K180016
access: public
definition: lux pR

BBa_B0034
type: Component
persistent identity: component2256440
display id: component2256440
version: 1
top level: BBa_K180016
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0015
type: Component
persistent identity: component2256451
display id: component2256451
version: 1
top level: BBa_K180016
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2256440
display id: annotation2256440
version: 1
top level: BBa_K180016
location: https://synbiohub.org/public/igem/BBa_K180016/annotation2256440/range2256440/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K180016/annotation2256440/range2256440/1
type: Range
persistent identity: range2256440
display id: range2256440
version: 1
direction: direction/reverse
top level: BBa_K180016
start: 64
end: 75
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2256451
display id: annotation2256451
version: 1
top level: BBa_K180016
location: https://synbiohub.org/public/igem/BBa_K180016/annotation2256451/range2256451/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K180016/annotation2256451/range2256451/1
type: Range
persistent identity: range2256451
display id: range2256451
version: 1
direction: direction/reverse
top level: BBa_K180016
start: 1243
end: 1371
orientation: inline

BBa_C0012
type: SequenceAnnotation
persistent identity: annotation2256442
display id: annotation2256442
version: 1
top level: BBa_K180016
location: https://synbiohub.org/public/igem/BBa_K180016/annotation2256442/range2256442/1
component: BBa_C0012

https://synbiohub.org/public/igem/BBa_K180016/annotation2256442/range2256442/1
type: Range
persistent identity: range2256442
display id: range2256442
version: 1
direction: direction/forward
top level: BBa_K180016
start: 82
end: 1209
orientation: inline

BBa_R0062
type: SequenceAnnotation
persistent identity: annotation2256434
display id: annotation2256434
version: 1
top level: BBa_K180016
location: https://synbiohub.org/public/igem/BBa_K180016/annotation2256434/range2256434/1
component: BBa_R0062

https://synbiohub.org/public/igem/BBa_K180016/annotation2256434/range2256434/1
type: Range
persistent identity: range2256434
display id: range2256434
version: 1
direction: direction/forward
top level: BBa_K180016
start: 1
end: 55
orientation: inline

BBa_J64010
type: ComponentDefinition
persistent identity: BBa J64010
display id: BBa_J64010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: lasI promoter
creator: Jeffrey J Tabor
created: 2007-01-15T12:00:00Z
modified: 2015-08-31T01:56:26Z
discontinued: false
dominant: false
experience: experience/None
group u list: _98_
m user id: 0
owner id: 88
owning group id: 98
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Promoter driving the lasI gene in Pseudomonas aeruginosa. Activated by LasR+3OC12HSL and not RhlR+C4HSL (Seed et al., 1995) ~4x stronger than the lasB promoter (BBa_R0079; Seed et al., 1995). Switch point ~0.1nM 3OC12HSL (Seed et al., 1995)
mutable notes: Promoter sequence cropped to 4nt upstream of Las box according to example of Whiteley and Greenberg, J. Bac, 2001
mutable provenance: Genomic sequence of Pseudomonas aeruginosa
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: Las box
sequence annotation: +1
sequence: https://synbiohub.org/public/igem/BBa_J64010_sequence/1

Las box
type: SequenceAnnotation
persistent identity: annotation1910615
display id: annotation1910615
version: 1
top level: BBa_J64010
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_J64010/annotation1910615/range1910615/1

https://synbiohub.org/public/igem/BBa_J64010/annotation1910615/range1910615/1
type: Range
persistent identity: range1910615
display id: range1910615
version: 1
direction: direction/forward
top level: BBa_J64010
start: 5
end: 20
orientation: inline

+1
type: SequenceAnnotation
persistent identity: annotation1910616
display id: annotation1910616
version: 1
top level: BBa_J64010
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_J64010/annotation1910616/range1910616/1

https://synbiohub.org/public/igem/BBa_J64010/annotation1910616/range1910616/1
type: Range
persistent identity: range1910616
display id: range1910616
version: 1
direction: direction/forward
top level: BBa_J64010
start: 53
end: 53
orientation: inline

BBa_K2168000
type: ComponentDefinition
persistent identity: BBa K2168000
display id: BBa_K2168000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pBAD/araC - N(6xHis) Ag43
creator: Arunavo Chakraborty
created: 2016-10-12T11:00:00Z
modified: 2016-10-13T02:44:12Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2639_
m user id: 30486
owner id: 30486
owning group id: 9
status: status/Planning
bookmark: true
mutable description: This part encodes E coli protein Ag43, with an additional 6xHis tag for easy purification and quantification. Ag43 expression is known to cause E coli cells to adhere together, and the 6xHis tag was added to allow for convenient study of variation of E coli aggregation with respect to Ag43 expression levels.
mutable notes: Ag43 is an outer membrane E coli protein, so it has a N-terminal 52 amino acid long signal peptide sequence, which is cleaved after Ag43 pro-protein is transported into the periplasmic space. Since strong dependence has been demonstrated on the signal peptide cleavage on the signal peptide-protein junction, the 6xHis tag should be added after Ala53, the residue immediately after the signal peptide sequence.
mutable provenance: This comes from Gibson assembly of fragments from a previous biobrick, BBa_K1352000 by Team:Aberdeen_Scotland, 2014.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Double terminator (B0015)
sequence annotation: 6xHis tag
sequence annotation: pBAD
sequence annotation: araC
sequence annotation: Ag43(PstI sites removed)
sequence annotation: B0034
sequence: https://synbiohub.org/public/igem/BBa_K2168000_sequence/1

Double terminator (B0015)
type: SequenceAnnotation
persistent identity: annotation2499426
display id: annotation2499426
version: 1
top level: BBa_K2168000
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K2168000/annotation2499426/range2499426/1

https://synbiohub.org/public/igem/BBa_K2168000/annotation2499426/range2499426/1
type: Range
persistent identity: range2499426
display id: range2499426
version: 1
direction: direction/forward
top level: BBa_K2168000
start: 4383
end: 4511
orientation: inline

6xHis tag
type: SequenceAnnotation
persistent identity: annotation2499423
display id: annotation2499423
version: 1
top level: BBa_K2168000
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K2168000/annotation2499423/range2499423/1

https://synbiohub.org/public/igem/BBa_K2168000/annotation2499423/range2499423/1
type: Range
persistent identity: range2499423
display id: range2499423
version: 1
direction: direction/forward
top level: BBa_K2168000
start: 1396
end: 1413
orientation: inline

pBAD
type: SequenceAnnotation
persistent identity: annotation2499411
display id: annotation2499411
version: 1
top level: BBa_K2168000
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K2168000/annotation2499411/range2499411/1

https://synbiohub.org/public/igem/BBa_K2168000/annotation2499411/range2499411/1
type: Range
persistent identity: range2499411
display id: range2499411
version: 1
direction: direction/forward
top level: BBa_K2168000
start: 906
end: 1190
orientation: inline

araC
type: SequenceAnnotation
persistent identity: annotation2499395
display id: annotation2499395
version: 1
top level: BBa_K2168000
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K2168000/annotation2499395/range2499395/1

https://synbiohub.org/public/igem/BBa_K2168000/annotation2499395/range2499395/1
type: Range
persistent identity: range2499395
display id: range2499395
version: 1
direction: direction/reverse
top level: BBa_K2168000
start: 1
end: 879
orientation: inline

Ag43(PstI sites removed)
type: SequenceAnnotation
persistent identity: annotation2499418
display id: annotation2499418
version: 1
top level: BBa_K2168000
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K2168000/annotation2499418/range2499418/1

https://synbiohub.org/public/igem/BBa_K2168000/annotation2499418/range2499418/1
type: Range
persistent identity: range2499418
display id: range2499418
version: 1
direction: direction/forward
top level: BBa_K2168000
start: 1237
end: 4374
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation2499416
display id: annotation2499416
version: 1
top level: BBa_K2168000
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K2168000/annotation2499416/range2499416/1

https://synbiohub.org/public/igem/BBa_K2168000/annotation2499416/range2499416/1
type: Range
persistent identity: range2499416
display id: range2499416
version: 1
direction: direction/forward
top level: BBa_K2168000
start: 1219
end: 1230
orientation: inline

pUV
type: ComponentDefinition
persistent identity: BBa I765001
display id: BBa_I765001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: UV promoter
creator: iGEM Colombian Team 2007
created: 2007-10-25T11:00:00Z
modified: 2015-08-31T04:08:09Z
discontinued: false
dominant: false
experience: experience/None
group u list: _118_
m user id: 0
owner id: 2266
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This device is regulated by UV irradiation
mutable notes: None
mutable provenance: Genomic sequence
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: promoter
sequence: https://synbiohub.org/public/igem/BBa_I765001_sequence/1

promoter
type: SequenceAnnotation
persistent identity: annotation1955941
display id: annotation1955941
version: 1
top level: pUV
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I765001/annotation1955941/range1955941/1

https://synbiohub.org/public/igem/BBa_I765001/annotation1955941/range1955941/1
type: Range
persistent identity: range1955941
display id: range1955941
version: 1
direction: direction/forward
top level: pUV
start: 1
end: 76
orientation: inline

BBa_J23119
type: ComponentDefinition
persistent identity: BBa J23119
display id: BBa_J23119
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: constitutive promoter family member
creator: John Anderson
created: 2006-08-23T11:00:00Z
modified: 2015-08-31T04:08:40Z
discontinued: false
dominant: true
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Later
mutable notes: N/A
mutable provenance: Overlap extension of synthetic oligonucleotides
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J23119_sequence/1

BBa_I718018
type: ComponentDefinition
persistent identity: BBa I718018
display id: BBa_I718018
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: dapAp promoter
creator: Eimad Shotar
created: 2007-10-25T11:00:00Z
modified: 2015-08-31T04:07:53Z
discontinued: false
dominant: false
group u list: _141_
m user id: 0
owner id: 1568
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: dapAp is the promter of dapA gene in E.coli. DapA enzyme is part of diaminopimelate (DAP)biosynthesis pathway. DAP is necessary for peptidoglycan and lysine biosynthesis. dapAp prmoter has been previously studied: Acord04: Acord J, Masters M (2004). "Expression from the Escherichia coli dapA promoter is regulated by intracellular levels of diaminopimelic acid." FEMS Microbiol Lett 235(1);131-7. PMID: 15158272 It has been shown that dapAp driven expression is inhibited at hight DAP. We have tried to confirm this by
mutable notes: None
mutable provenance: none yet
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I718018_sequence/1

BBa_K1520025
type: ComponentDefinition
persistent identity: BBa K1520025
display id: BBa_K1520025
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ptet-rbs-rfp-Ter-Plac-rbs-tetR-Ter
creator: yue wu
created: 2014-09-21T11:00:00Z
modified: 2015-05-08T01:10:49Z
discontinued: false
dominant: false
group u list: _1902_
m user id: 0
owner id: 21318
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Ptet-rbs-rfp-Ter-Plac-rbs-tetR-Ter. It produces rfp when there is enough lacI or less lactose.
mutable notes: None
mutable provenance: Ptet, rfp, Plac, tetR, Ter
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1520014
component: BBa_K1520015
sequence annotation: BBa_K1520014
sequence annotation: BBa_K1520015
sequence: https://synbiohub.org/public/igem/BBa_K1520025_sequence/1

BBa_K1520014
type: Component
persistent identity: component2385272
display id: component2385272
version: 1
top level: BBa_K1520025
access: public
definition: BBa_K1520014

BBa_K1520015
type: Component
persistent identity: component2385284
display id: component2385284
version: 1
top level: BBa_K1520025
access: public
definition: https://synbiohub.org/public/igem/BBa_K1520015/1

BBa_K1520014
type: SequenceAnnotation
persistent identity: annotation2385272
display id: annotation2385272
version: 1
top level: BBa_K1520025
location: https://synbiohub.org/public/igem/BBa_K1520025/annotation2385272/range2385272/1
component: BBa_K1520014

https://synbiohub.org/public/igem/BBa_K1520025/annotation2385272/range2385272/1
type: Range
persistent identity: range2385272
display id: range2385272
version: 1
direction: direction/reverse
top level: BBa_K1520025
start: 1
end: 931
orientation: inline

BBa_K1520015
type: SequenceAnnotation
persistent identity: annotation2385284
display id: annotation2385284
version: 1
top level: BBa_K1520025
location: https://synbiohub.org/public/igem/BBa_K1520025/annotation2385284/range2385284/1
component: BBa_K1520015

https://synbiohub.org/public/igem/BBa_K1520025/annotation2385284/range2385284/1
type: Range
persistent identity: range2385284
display id: range2385284
version: 1
direction: direction/reverse
top level: BBa_K1520025
start: 940
end: 1987
orientation: inline

BBa_J64987
type: ComponentDefinition
persistent identity: BBa J64987
display id: BBa_J64987
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LacI Consensus Binding Site in sigma 70 binding region
creator: Sai Duriseti
created: 2007-03-25T11:00:00Z
modified: 2015-05-08T01:08:18Z
discontinued: false
dominant: false
experience: experience/None
group u list: _98_
m user id: 0
owner id: 1428
owning group id: 98
sample status: Not in stock
status: status/Unavailable
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: LacI
sequence annotation: -10
sequence annotation: -35
sequence: https://synbiohub.org/public/igem/BBa_J64987_sequence/1

LacI
type: SequenceAnnotation
persistent identity: annotation1921610
display id: annotation1921610
version: 1
top level: BBa_J64987
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_J64987/annotation1921610/range1921610/1

https://synbiohub.org/public/igem/BBa_J64987/annotation1921610/range1921610/1
type: Range
persistent identity: range1921610
display id: range1921610
version: 1
direction: direction/forward
top level: BBa_J64987
start: 7
end: 26
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1921609
display id: annotation1921609
version: 1
top level: BBa_J64987
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J64987/annotation1921609/range1921609/1

https://synbiohub.org/public/igem/BBa_J64987/annotation1921609/range1921609/1
type: Range
persistent identity: range1921609
display id: range1921609
version: 1
direction: direction/forward
top level: BBa_J64987
start: 27
end: 32
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1921608
display id: annotation1921608
version: 1
top level: BBa_J64987
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J64987/annotation1921608/range1921608/1

https://synbiohub.org/public/igem/BBa_J64987/annotation1921608/range1921608/1
type: Range
persistent identity: range1921608
display id: range1921608
version: 1
direction: direction/forward
top level: BBa_J64987
start: 1
end: 6
orientation: inline

BBa_I739104
type: ComponentDefinition
persistent identity: BBa I739104
display id: BBa_I739104
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Double Promoter (LuxR/HSL, positive / P22 cII, negative)
creator: Stefan Luzi
created: 2007-10-14T11:00:00Z
modified: 2015-08-31T04:08:01Z
discontinued: false
dominant: false
group u list: _124_
m user id: 0
owner id: 2183
owning group id: 62
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: asdf
mutable notes: asdf
mutable provenance: Synthetic DNA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: R0053 (2x OR1)
sequence annotation: R0062
sequence annotation: OR1
sequence annotation: Scar
sequence: https://synbiohub.org/public/igem/BBa_I739104_sequence/1

R0053 (2x OR1)
type: SequenceAnnotation
persistent identity: annotation1947895
display id: annotation1947895
version: 1
top level: BBa_I739104
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_I739104/annotation1947895/range1947895/1

https://synbiohub.org/public/igem/BBa_I739104/annotation1947895/range1947895/1
type: Range
persistent identity: range1947895
display id: range1947895
version: 1
direction: direction/forward
top level: BBa_I739104
start: 64
end: 101
orientation: inline

R0062
type: SequenceAnnotation
persistent identity: annotation1947891
display id: annotation1947891
version: 1
top level: BBa_I739104
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I739104/annotation1947891/range1947891/1

https://synbiohub.org/public/igem/BBa_I739104/annotation1947891/range1947891/1
type: Range
persistent identity: range1947891
display id: range1947891
version: 1
direction: direction/forward
top level: BBa_I739104
start: 1
end: 55
orientation: inline

OR1
type: SequenceAnnotation
persistent identity: annotation1947894
display id: annotation1947894
version: 1
top level: BBa_I739104
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I739104/annotation1947894/range1947894/1

https://synbiohub.org/public/igem/BBa_I739104/annotation1947894/range1947894/1
type: Range
persistent identity: range1947894
display id: range1947894
version: 1
direction: direction/forward
top level: BBa_I739104
start: 83
end: 101
orientation: inline

Scar
type: SequenceAnnotation
persistent identity: annotation1947892
display id: annotation1947892
version: 1
top level: BBa_I739104
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I739104/annotation1947892/range1947892/1

https://synbiohub.org/public/igem/BBa_I739104/annotation1947892/range1947892/1
type: Range
persistent identity: range1947892
display id: range1947892
version: 1
direction: direction/forward
top level: BBa_I739104
start: 56
end: 63
orientation: inline

OR1
type: SequenceAnnotation
persistent identity: annotation1947893
display id: annotation1947893
version: 1
top level: BBa_I739104
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I739104/annotation1947893/range1947893/1

https://synbiohub.org/public/igem/BBa_I739104/annotation1947893/range1947893/1
type: Range
persistent identity: range1947893
display id: range1947893
version: 1
direction: direction/forward
top level: BBa_I739104
start: 64
end: 82
orientation: inline

U037O11+D0
type: ComponentDefinition
persistent identity: BBa I732444
display id: BBa_I732444
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member (U037O11+D014O22)
creator: Zhan Jian
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732444_sequence/1

BBa_K137029
type: ComponentDefinition
persistent identity: BBa K137029
display id: BBa_K137029
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: constitutive promoter with (TA)10 between -10 and -35 elements
creator: Allen Lin
created: 2008-07-20T11:00:00Z
modified: 2015-05-08T01:10:09Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _187_
m user id: 0
owner id: 3112
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: constitutive promoter with (TA)10 between -10 and -35 elements
mutable notes: With 10 TA repeats between the -10 and -35 elements, the distance between the two elements is optimal for the sigma factor to bind to the promoter. Thus, the promoter should be in the 'on' state.
mutable provenance: This part was PCR amplified from two synthesized primers that bound to each other.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: TA repeat
sequence annotation: -10 element
sequence: https://synbiohub.org/public/igem/BBa_K137029_sequence/1

TA repeat
type: SequenceAnnotation
persistent identity: annotation1967765
display id: annotation1967765
version: 1
top level: BBa_K137029
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K137029/annotation1967765/range1967765/1

https://synbiohub.org/public/igem/BBa_K137029/annotation1967765/range1967765/1
type: Range
persistent identity: range1967765
display id: range1967765
version: 1
direction: direction/forward
top level: BBa_K137029
start: 7
end: 22
orientation: inline

-10 element
type: SequenceAnnotation
persistent identity: annotation1967764
display id: annotation1967764
version: 1
top level: BBa_K137029
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K137029/annotation1967764/range1967764/1

https://synbiohub.org/public/igem/BBa_K137029/annotation1967764/range1967764/1
type: Range
persistent identity: range1967764
display id: range1967764
version: 1
direction: direction/forward
top level: BBa_K137029
start: 23
end: 28
orientation: inline

BBa_K180011
type: ComponentDefinition
persistent identity: BBa K180011
display id: BBa_K180011
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Rock - Primary plasmid (part 1) [RhlR generator]
creator: Richard Wilson, Stephanie Chambers
created: 2009-10-18T11:00:00Z
modified: 2015-05-08T01:11:06Z
discontinued: false
dominant: false
experience: experience/None
group u list: _278_
m user id: 0
owner id: 4082
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Part of the iGEM2009 Team:Southampton rock-paper-scissors 'rock' primary plasmid.
mutable notes: Constitutive promoter selected to allow for continuous expression of C0171.
mutable provenance: See relevant basic biobrick parts (J23119, B0034, C0171, B0015)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0012
component: BBa_C0171
component: BBa_B0034
component: BBa_J23119
component: BBa_B0010
sequence annotation: BBa_J23119
sequence annotation: BBa_B0010
sequence annotation: BBa_C0171
sequence annotation: BBa_B0034
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_K180011_sequence/1

BBa_B0012
type: Component
persistent identity: component2053908
display id: component2053908
version: 1
top level: BBa_K180011
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_C0171
type: Component
persistent identity: component2053905
display id: component2053905
version: 1
top level: BBa_K180011
access: public
definition: https://synbiohub.org/public/igem/BBa_C0171/1

BBa_B0034
type: Component
persistent identity: component2053904
display id: component2053904
version: 1
top level: BBa_K180011
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_J23119
type: Component
persistent identity: component2053902
display id: component2053902
version: 1
top level: BBa_K180011
access: public
definition: BBa_J23119

BBa_B0010
type: Component
persistent identity: component2053906
display id: component2053906
version: 1
top level: BBa_K180011
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_J23119
type: SequenceAnnotation
persistent identity: annotation2053902
display id: annotation2053902
version: 1
top level: BBa_K180011
location: https://synbiohub.org/public/igem/BBa_K180011/annotation2053902/range2053902/1
component: BBa_J23119

https://synbiohub.org/public/igem/BBa_K180011/annotation2053902/range2053902/1
type: Range
persistent identity: range2053902
display id: range2053902
version: 1
direction: direction/reverse
top level: BBa_K180011
start: 1
end: 35
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2053906
display id: annotation2053906
version: 1
top level: BBa_K180011
location: https://synbiohub.org/public/igem/BBa_K180011/annotation2053906/range2053906/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K180011/annotation2053906/range2053906/1
type: Range
persistent identity: range2053906
display id: range2053906
version: 1
direction: direction/forward
top level: BBa_K180011
start: 799
end: 878
orientation: inline

BBa_C0171
type: SequenceAnnotation
persistent identity: annotation2053905
display id: annotation2053905
version: 1
top level: BBa_K180011
location: https://synbiohub.org/public/igem/BBa_K180011/annotation2053905/range2053905/1
component: BBa_C0171

https://synbiohub.org/public/igem/BBa_K180011/annotation2053905/range2053905/1
type: Range
persistent identity: range2053905
display id: range2053905
version: 1
direction: direction/reverse
top level: BBa_K180011
start: 62
end: 790
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2053904
display id: annotation2053904
version: 1
top level: BBa_K180011
location: https://synbiohub.org/public/igem/BBa_K180011/annotation2053904/range2053904/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K180011/annotation2053904/range2053904/1
type: Range
persistent identity: range2053904
display id: range2053904
version: 1
direction: direction/reverse
top level: BBa_K180011
start: 44
end: 55
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2053908
display id: annotation2053908
version: 1
top level: BBa_K180011
location: https://synbiohub.org/public/igem/BBa_K180011/annotation2053908/range2053908/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K180011/annotation2053908/range2053908/1
type: Range
persistent identity: range2053908
display id: range2053908
version: 1
direction: direction/forward
top level: BBa_K180011
start: 887
end: 927
orientation: inline

BBa_K091143
type: ComponentDefinition
persistent identity: BBa K091143
display id: BBa_K091143
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pLas/cI Hybrid Promoter
creator: Xiao Zhu
created: 2008-07-14T11:00:00Z
modified: 2015-05-08T01:08:38Z
discontinued: false
dominant: false
experience: experience/None
group u list: _191_
m user id: 0
owner id: 3130
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: this hybrid promoter is designed to be consecutively off.It combines with plasR(R0079) and lambda cI OR1(see C0051).Adding P-AI-1 sould activate the promoter, while repressed by lambda cI.
mutable notes: "CI binding of the OR region. It binds OR1 with highest affinity, OR2 with next highest, and OR3 with lowest"(http://www.nonanone.com/qw/files/Biology%204450/test2_lecture8,9.txt)
mutable provenance: .
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: plasR OP2
sequence annotation: -10
sequence annotation: -35
sequence annotation: cI OR1
sequence annotation: plasR OP1
sequence: https://synbiohub.org/public/igem/BBa_K091143_sequence/1

plasR OP2
type: SequenceAnnotation
persistent identity: annotation1967108
display id: annotation1967108
version: 1
top level: BBa_K091143
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K091143/annotation1967108/range1967108/1

https://synbiohub.org/public/igem/BBa_K091143/annotation1967108/range1967108/1
type: Range
persistent identity: range1967108
display id: range1967108
version: 1
direction: direction/forward
top level: BBa_K091143
start: 106
end: 126
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1967104
display id: annotation1967104
version: 1
top level: BBa_K091143
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K091143/annotation1967104/range1967104/1

https://synbiohub.org/public/igem/BBa_K091143/annotation1967104/range1967104/1
type: Range
persistent identity: range1967104
display id: range1967104
version: 1
direction: direction/forward
top level: BBa_K091143
start: 140
end: 145
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1967105
display id: annotation1967105
version: 1
top level: BBa_K091143
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K091143/annotation1967105/range1967105/1

https://synbiohub.org/public/igem/BBa_K091143/annotation1967105/range1967105/1
type: Range
persistent identity: range1967105
display id: range1967105
version: 1
direction: direction/forward
top level: BBa_K091143
start: 117
end: 122
orientation: inline

cI OR1
type: SequenceAnnotation
persistent identity: annotation1967106
display id: annotation1967106
version: 1
top level: BBa_K091143
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K091143/annotation1967106/range1967106/1

https://synbiohub.org/public/igem/BBa_K091143/annotation1967106/range1967106/1
type: Range
persistent identity: range1967106
display id: range1967106
version: 1
direction: direction/forward
top level: BBa_K091143
start: 147
end: 164
orientation: inline

plasR OP1
type: SequenceAnnotation
persistent identity: annotation1967107
display id: annotation1967107
version: 1
top level: BBa_K091143
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K091143/annotation1967107/range1967107/1

https://synbiohub.org/public/igem/BBa_K091143/annotation1967107/range1967107/1
type: Range
persistent identity: range1967107
display id: range1967107
version: 1
direction: direction/forward
top level: BBa_K091143
start: 46
end: 63
orientation: inline

BBa_K1520014
type: ComponentDefinition
persistent identity: BBa K1520014
display id: BBa_K1520014
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ptet-rbs-rfp-Ter
creator: yue wu
created: 2014-09-21T11:00:00Z
modified: 2015-05-08T01:10:49Z
discontinued: false
dominant: false
group u list: _1902_
m user id: 0
owner id: 21318
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Ptet-rbs-rfp-Ter. It produces rfp when there is less tetR.
mutable notes: None
mutable provenance: Ptet, rfp, Ter
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_K1520500
component: BBa_K1520029
component: BBa_R0040
sequence annotation: BBa_K1520500
sequence annotation: BBa_R0040
sequence annotation: BBa_K1520029
sequence: https://synbiohub.org/public/igem/BBa_K1520014_sequence/1

BBa_K1520500
type: Component
persistent identity: component2384997
display id: component2384997
version: 1
top level: BBa_K1520014
access: public
definition: https://synbiohub.org/public/igem/BBa_K1520500/1

BBa_K1520029
type: Component
persistent identity: component2384998
display id: component2384998
version: 1
top level: BBa_K1520014
access: public
definition: https://synbiohub.org/public/igem/BBa_K1520029/1

BBa_R0040
type: Component
persistent identity: component2384992
display id: component2384992
version: 1
top level: BBa_K1520014
access: public
definition: p(tetR)

BBa_K1520500
type: SequenceAnnotation
persistent identity: annotation2384997
display id: annotation2384997
version: 1
top level: BBa_K1520014
location: https://synbiohub.org/public/igem/BBa_K1520014/annotation2384997/range2384997/1
component: BBa_K1520500

https://synbiohub.org/public/igem/BBa_K1520014/annotation2384997/range2384997/1
type: Range
persistent identity: range2384997
display id: range2384997
version: 1
direction: direction/reverse
top level: BBa_K1520014
start: 63
end: 794
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2384992
display id: annotation2384992
version: 1
top level: BBa_K1520014
location: https://synbiohub.org/public/igem/BBa_K1520014/annotation2384992/range2384992/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_K1520014/annotation2384992/range2384992/1
type: Range
persistent identity: range2384992
display id: range2384992
version: 1
direction: direction/forward
top level: BBa_K1520014
start: 1
end: 54
orientation: inline

BBa_K1520029
type: SequenceAnnotation
persistent identity: annotation2384998
display id: annotation2384998
version: 1
top level: BBa_K1520014
location: https://synbiohub.org/public/igem/BBa_K1520014/annotation2384998/range2384998/1
component: BBa_K1520029

https://synbiohub.org/public/igem/BBa_K1520014/annotation2384998/range2384998/1
type: Range
persistent identity: range2384998
display id: range2384998
version: 1
direction: direction/reverse
top level: BBa_K1520014
start: 803
end: 931
orientation: inline

BBa_J23107
type: ComponentDefinition
persistent identity: BBa J23107
display id: BBa_J23107
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: constitutive promoter family member
creator: John Anderson
created: 2006-08-16T11:00:00Z
modified: 2015-08-31T04:08:40Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: Later
mutable notes: N/A
mutable provenance: Later
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J23107_sequence/1

BBa_J64981
type: ComponentDefinition
persistent identity: BBa J64981
display id: BBa_J64981
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: OmpR-P strong binding, regulatory region for Team Challenge03-2007
creator: Sai Duriseti
created: 2007-03-25T11:00:00Z
modified: 2015-05-08T01:08:18Z
discontinued: false
dominant: false
group u list: _98_
m user id: 0
owner id: 1428
owning group id: 98
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Contains the F1 consensus site from the OmpF regulator. Contains a LacI repressor consensus binding site between the -35 and -10 portions of a T7 core promoter region, and a TetR consensus binding site upstream of the -35 site.
mutable notes: The weaker binding repressor binds upstream of the core promoter (upstream of -35), while the stronger binds inbetween the -35 and -10.
mutable provenance: Information for creation of this construct came from the following sorces: Yoshida et al. JBC 2006 Frank et al. JMB 1997 Rong et al. PNAS 1998 Merabet et al. Biochemistry 1995 Su et al. JBC 1998 Kedracka-Krok et al. JPC 1999 Kleinschmidt et al. Biochemistry 1988 Butler et al. JBC 1982 Jorgensen et al. JBC 1991 Hillen et al. JMB 1984 Head et al. JMB 1998
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -10
sequence annotation: T7 RNA Polymerase Recognition
sequence annotation: Strong OmpR
sequence annotation: LacI Consensus
sequence annotation: -35
sequence annotation: TetR Consensus
sequence: https://synbiohub.org/public/igem/BBa_J64981_sequence/1

-10
type: SequenceAnnotation
persistent identity: annotation1921603
display id: annotation1921603
version: 1
top level: BBa_J64981
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J64981/annotation1921603/range1921603/1

https://synbiohub.org/public/igem/BBa_J64981/annotation1921603/range1921603/1
type: Range
persistent identity: range1921603
display id: range1921603
version: 1
direction: direction/forward
top level: BBa_J64981
start: 66
end: 70
orientation: inline

T7 RNA Polymerase Recognition
type: SequenceAnnotation
persistent identity: annotation1921600
display id: annotation1921600
version: 1
top level: BBa_J64981
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J64981/annotation1921600/range1921600/1

https://synbiohub.org/public/igem/BBa_J64981/annotation1921600/range1921600/1
type: Range
persistent identity: range1921600
display id: range1921600
version: 1
direction: direction/forward
top level: BBa_J64981
start: 40
end: 82
orientation: inline

Strong OmpR
type: SequenceAnnotation
persistent identity: annotation1921598
display id: annotation1921598
version: 1
top level: BBa_J64981
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_J64981/annotation1921598/range1921598/1

https://synbiohub.org/public/igem/BBa_J64981/annotation1921598/range1921598/1
type: Range
persistent identity: range1921598
display id: range1921598
version: 1
direction: direction/forward
top level: BBa_J64981
start: 1
end: 20
orientation: inline

LacI Consensus
type: SequenceAnnotation
persistent identity: annotation1921601
display id: annotation1921601
version: 1
top level: BBa_J64981
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_J64981/annotation1921601/range1921601/1

https://synbiohub.org/public/igem/BBa_J64981/annotation1921601/range1921601/1
type: Range
persistent identity: range1921601
display id: range1921601
version: 1
direction: direction/forward
top level: BBa_J64981
start: 46
end: 65
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1921602
display id: annotation1921602
version: 1
top level: BBa_J64981
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J64981/annotation1921602/range1921602/1

https://synbiohub.org/public/igem/BBa_J64981/annotation1921602/range1921602/1
type: Range
persistent identity: range1921602
display id: range1921602
version: 1
direction: direction/forward
top level: BBa_J64981
start: 40
end: 45
orientation: inline

TetR Consensus
type: SequenceAnnotation
persistent identity: annotation1921599
display id: annotation1921599
version: 1
top level: BBa_J64981
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_J64981/annotation1921599/range1921599/1

https://synbiohub.org/public/igem/BBa_J64981/annotation1921599/range1921599/1
type: Range
persistent identity: range1921599
display id: range1921599
version: 1
direction: direction/forward
top level: BBa_J64981
start: 21
end: 39
orientation: inline

BBa_K731201
type: ComponentDefinition
persistent identity: BBa K731201
display id: BBa_K731201
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Arabinose inducible araC-pBAD promoter
creator: Daniele Rossetto
created: 2012-08-02T11:00:00Z
modified: 2015-05-08T01:13:06Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _977_
m user id: 0
owner id: 11997
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: This E. coli promoter is positively regulated by arabinose. araC is the regulator protein, binding to arabinose prevents the repression of pBAD.
mutable notes: This part is similar to BBa_I0500, but it differs from that one in several point mutations in the sequence.
mutable provenance: E. coli genome
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: pBAD
sequence annotation: araC
sequence annotation: PC
sequence: https://synbiohub.org/public/igem/BBa_K731201_sequence/1

pBAD
type: SequenceAnnotation
persistent identity: annotation2179311
display id: annotation2179311
version: 1
top level: BBa_K731201
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K731201/annotation2179311/range2179311/1

https://synbiohub.org/public/igem/BBa_K731201/annotation2179311/range2179311/1
type: Range
persistent identity: range2179311
display id: range2179311
version: 1
direction: direction/forward
top level: BBa_K731201
start: 1080
end: 1203
orientation: inline

araC
type: SequenceAnnotation
persistent identity: annotation2179313
display id: annotation2179313
version: 1
top level: BBa_K731201
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K731201/annotation2179313/range2179313/1

https://synbiohub.org/public/igem/BBa_K731201/annotation2179313/range2179313/1
type: Range
persistent identity: range2179313
display id: range2179313
version: 1
direction: direction/reverse
top level: BBa_K731201
start: 1
end: 1080
orientation: inline

PC
type: SequenceAnnotation
persistent identity: annotation2179312
display id: annotation2179312
version: 1
top level: BBa_K731201
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K731201/annotation2179312/range2179312/1

https://synbiohub.org/public/igem/BBa_K731201/annotation2179312/range2179312/1
type: Range
persistent identity: range2179312
display id: range2179312
version: 1
direction: direction/reverse
top level: BBa_K731201
start: 1043
end: 1114
orientation: inline

BBa_K091100
type: ComponentDefinition
persistent identity: BBa K091100
display id: BBa_K091100
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pLac_lux hybrid promoter
creator: Pallavi Penumetcha
created: 2008-05-28T11:00:00Z
modified: 2015-05-08T01:08:37Z
discontinued: false
dominant: false
experience: experience/None
group u list: _191_
m user id: 0
owner id: 2993
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This promoter is repressed by lacI and luxR with HSL. It contains a lux box and a lacI binding site.
mutable notes: Deleted the CAP binding site in R0010.
mutable provenance: R0010 modified with the insertion of a lux box in between -35 and -10.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: lacI binding site
sequence annotation: lux box
sequence annotation: -10
sequence annotation: -35
sequence: https://synbiohub.org/public/igem/BBa_K091100_sequence/1

lacI binding site
type: SequenceAnnotation
persistent identity: annotation1963336
display id: annotation1963336
version: 1
top level: BBa_K091100
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K091100/annotation1963336/range1963336/1

https://synbiohub.org/public/igem/BBa_K091100/annotation1963336/range1963336/1
type: Range
persistent identity: range1963336
display id: range1963336
version: 1
direction: direction/forward
top level: BBa_K091100
start: 40
end: 74
orientation: inline

lux box
type: SequenceAnnotation
persistent identity: annotation1963335
display id: annotation1963335
version: 1
top level: BBa_K091100
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K091100/annotation1963335/range1963335/1

https://synbiohub.org/public/igem/BBa_K091100/annotation1963335/range1963335/1
type: Range
persistent identity: range1963335
display id: range1963335
version: 1
direction: direction/forward
top level: BBa_K091100
start: 16
end: 33
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1963334
display id: annotation1963334
version: 1
top level: BBa_K091100
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K091100/annotation1963334/range1963334/1

https://synbiohub.org/public/igem/BBa_K091100/annotation1963334/range1963334/1
type: Range
persistent identity: range1963334
display id: range1963334
version: 1
direction: direction/forward
top level: BBa_K091100
start: 34
end: 39
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1963333
display id: annotation1963333
version: 1
top level: BBa_K091100
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K091100/annotation1963333/range1963333/1

https://synbiohub.org/public/igem/BBa_K091100/annotation1963333/range1963333/1
type: Range
persistent identity: range1963333
display id: range1963333
version: 1
direction: direction/forward
top level: BBa_K091100
start: 10
end: 15
orientation: inline

BBa_J34800
type: ComponentDefinition
persistent identity: BBa J34800
display id: BBa_J34800
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter tetracyclin inducible
creator: Alexandra Choutko
created: 2006-10-25T11:00:00Z
modified: 2015-08-31T04:08:46Z
discontinued: false
dominant: false
group u list: _62_
m user id: 0
owner id: 1085
owning group id: 62
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: promoter
mutable notes: no
mutable provenance: no
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J34800_sequence/1

BBa_K101001
type: ComponentDefinition
persistent identity: BBa K101001
display id: BBa_K101001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Dual-Repressed Promoter for LacI and LambdacI
creator: Bennett Swiniarski
created: 2008-07-27T11:00:00Z
modified: 2015-05-08T01:08:41Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _205_
m user id: 0
owner id: 3363
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This part is a new construct of a promoter that is dually repressed by either LacI or LambdacI proteins.
mutable notes: LambdacI usually binds to two operator sites, one located upstream from -35 and one located between -35 and -10. By itself, LacI binds downstream from -10. This made the part design very straightforward, since the locations of the operator sites do not overlap at all. In order to complete this part, we simply combined the proper sequences and we had a dually-repressed promoter.
mutable provenance: This part was made by combining BioBrick parts R0010(LacI promoter) and R0051 (LambdacI promoter), along with intermediate sequence between these operator sites.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K101001_sequence/1

U097NUL+D0
type: ComponentDefinition
persistent identity: BBa I732409
display id: BBa_I732409
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member (U097NUL+D026O22)
creator: Zhan Jian
created: 2007-09-19T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1572
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: A Double Reporter (LacZ-alpha and GFP) loaded on pSB3C5. For more information please refer to BBa_I732403.
mutable notes: Might be later edited.
mutable provenance: Might be later edited.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BBa_B0010
sequence annotation: GFP protein
sequence annotation: lacZ
sequence annotation: BBa_B0034
sequence annotation: BamHI
sequence annotation: conserved
sequence annotation: STOP
sequence annotation: stem_loop
sequence annotation: START
sequence annotation: BBa_I732006
sequence annotation: stop
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: BglII
sequence annotation: BBa_E0040
sequence annotation: BBa_I732007
sequence annotation: polya
sequence: https://synbiohub.org/public/igem/BBa_I732409_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1944530
display id: annotation1944530
version: 1
top level: U097NUL+D0
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I732409/annotation1944530/range1944530/1

https://synbiohub.org/public/igem/BBa_I732409/annotation1944530/range1944530/1
type: Range
persistent identity: range1944530
display id: range1944530
version: 1
direction: direction/forward
top level: U097NUL+D0
start: 1034
end: 1113
orientation: inline

GFP protein
type: SequenceAnnotation
persistent identity: annotation1944528
display id: annotation1944528
version: 1
top level: U097NUL+D0
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_I732409/annotation1944528/range1944528/1

https://synbiohub.org/public/igem/BBa_I732409/annotation1944528/range1944528/1
type: Range
persistent identity: range1944528
display id: range1944528
version: 1
direction: direction/forward
top level: U097NUL+D0
start: 306
end: 1025
orientation: inline

lacZ
type: SequenceAnnotation
persistent identity: annotation1944523
display id: annotation1944523
version: 1
top level: U097NUL+D0
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_I732409/annotation1944523/range1944523/1

https://synbiohub.org/public/igem/BBa_I732409/annotation1944523/range1944523/1
type: Range
persistent identity: range1944523
display id: range1944523
version: 1
direction: direction/forward
top level: U097NUL+D0
start: 46
end: 279
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1944527
display id: annotation1944527
version: 1
top level: U097NUL+D0
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I732409/annotation1944527/range1944527/1

https://synbiohub.org/public/igem/BBa_I732409/annotation1944527/range1944527/1
type: Range
persistent identity: range1944527
display id: range1944527
version: 1
direction: direction/reverse
top level: U097NUL+D0
start: 288
end: 299
orientation: inline

BamHI
type: SequenceAnnotation
persistent identity: annotation1944518
display id: annotation1944518
version: 1
top level: U097NUL+D0
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I732409/annotation1944518/range1944518/1

https://synbiohub.org/public/igem/BBa_I732409/annotation1944518/range1944518/1
type: Range
persistent identity: range1944518
display id: range1944518
version: 1
direction: direction/forward
top level: U097NUL+D0
start: 12
end: 17
orientation: inline

conserved
type: SequenceAnnotation
persistent identity: annotation1944520
display id: annotation1944520
version: 1
top level: U097NUL+D0
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_I732409/annotation1944520/range1944520/1

https://synbiohub.org/public/igem/BBa_I732409/annotation1944520/range1944520/1
type: Range
persistent identity: range1944520
display id: range1944520
version: 1
direction: direction/forward
top level: U097NUL+D0
start: 32
end: 35
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1944521
display id: annotation1944521
version: 1
top level: U097NUL+D0
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I732409/annotation1944521/range1944521/1

https://synbiohub.org/public/igem/BBa_I732409/annotation1944521/range1944521/1
type: Range
persistent identity: range1944521
display id: range1944521
version: 1
direction: direction/reverse
top level: U097NUL+D0
start: 28
end: 39
orientation: inline

STOP
type: SequenceAnnotation
persistent identity: annotation1944524
display id: annotation1944524
version: 1
top level: U097NUL+D0
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_I732409/annotation1944524/range1944524/1

https://synbiohub.org/public/igem/BBa_I732409/annotation1944524/range1944524/1
type: Range
persistent identity: range1944524
display id: range1944524
version: 1
direction: direction/forward
top level: U097NUL+D0
start: 274
end: 279
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation1944531
display id: annotation1944531
version: 1
top level: U097NUL+D0
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_I732409/annotation1944531/range1944531/1

https://synbiohub.org/public/igem/BBa_I732409/annotation1944531/range1944531/1
type: Range
persistent identity: range1944531
display id: range1944531
version: 1
direction: direction/forward
top level: U097NUL+D0
start: 1045
end: 1088
orientation: inline

START
type: SequenceAnnotation
persistent identity: annotation1944522
display id: annotation1944522
version: 1
top level: U097NUL+D0
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_I732409/annotation1944522/range1944522/1

https://synbiohub.org/public/igem/BBa_I732409/annotation1944522/range1944522/1
type: Range
persistent identity: range1944522
display id: range1944522
version: 1
direction: direction/forward
top level: U097NUL+D0
start: 46
end: 48
orientation: inline

BBa_I732006
type: SequenceAnnotation
persistent identity: annotation1944525
display id: annotation1944525
version: 1
top level: U097NUL+D0
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I732409/annotation1944525/range1944525/1

https://synbiohub.org/public/igem/BBa_I732409/annotation1944525/range1944525/1
type: Range
persistent identity: range1944525
display id: range1944525
version: 1
direction: direction/reverse
top level: U097NUL+D0
start: 46
end: 279
orientation: inline

conserved
type: SequenceAnnotation
persistent identity: annotation1944526
display id: annotation1944526
version: 1
top level: U097NUL+D0
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_I732409/annotation1944526/range1944526/1

https://synbiohub.org/public/igem/BBa_I732409/annotation1944526/range1944526/1
type: Range
persistent identity: range1944526
display id: range1944526
version: 1
direction: direction/forward
top level: U097NUL+D0
start: 292
end: 295
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1944535
display id: annotation1944535
version: 1
top level: U097NUL+D0
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_I732409/annotation1944535/range1944535/1

https://synbiohub.org/public/igem/BBa_I732409/annotation1944535/range1944535/1
type: Range
persistent identity: range1944535
display id: range1944535
version: 1
direction: direction/forward
top level: U097NUL+D0
start: 1155
end: 1155
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1944532
display id: annotation1944532
version: 1
top level: U097NUL+D0
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I732409/annotation1944532/range1944532/1

https://synbiohub.org/public/igem/BBa_I732409/annotation1944532/range1944532/1
type: Range
persistent identity: range1944532
display id: range1944532
version: 1
direction: direction/forward
top level: U097NUL+D0
start: 1122
end: 1162
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1944533
display id: annotation1944533
version: 1
top level: U097NUL+D0
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_I732409/annotation1944533/range1944533/1

https://synbiohub.org/public/igem/BBa_I732409/annotation1944533/range1944533/1
type: Range
persistent identity: range1944533
display id: range1944533
version: 1
direction: direction/forward
top level: U097NUL+D0
start: 1129
end: 1148
orientation: inline

BglII
type: SequenceAnnotation
persistent identity: annotation1944517
display id: annotation1944517
version: 1
top level: U097NUL+D0
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I732409/annotation1944517/range1944517/1

https://synbiohub.org/public/igem/BBa_I732409/annotation1944517/range1944517/1
type: Range
persistent identity: range1944517
display id: range1944517
version: 1
direction: direction/forward
top level: U097NUL+D0
start: 3
end: 8
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation1944529
display id: annotation1944529
version: 1
top level: U097NUL+D0
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I732409/annotation1944529/range1944529/1

https://synbiohub.org/public/igem/BBa_I732409/annotation1944529/range1944529/1
type: Range
persistent identity: range1944529
display id: range1944529
version: 1
direction: direction/reverse
top level: U097NUL+D0
start: 306
end: 1025
orientation: inline

BBa_I732007
type: SequenceAnnotation
persistent identity: annotation1944519
display id: annotation1944519
version: 1
top level: U097NUL+D0
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I732409/annotation1944519/range1944519/1

https://synbiohub.org/public/igem/BBa_I732409/annotation1944519/range1944519/1
type: Range
persistent identity: range1944519
display id: range1944519
version: 1
direction: direction/reverse
top level: U097NUL+D0
start: 1
end: 19
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1944534
display id: annotation1944534
version: 1
top level: U097NUL+D0
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_I732409/annotation1944534/range1944534/1

https://synbiohub.org/public/igem/BBa_I732409/annotation1944534/range1944534/1
type: Range
persistent identity: range1944534
display id: range1944534
version: 1
direction: direction/forward
top level: U097NUL+D0
start: 1149
end: 1162
orientation: inline

cI HK022
type: ComponentDefinition
persistent identity: BBa R0050
display id: BBa_R0050
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter (HK022 cI regulated)
creator: Reshma Shetty
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: The pR regulatory region is a 98 base-pair sequence with standard BioBrick prefix and suffix sections on its ends. It contains two operator sites at which HK022 cI protein can bind cooperatively. The -35 and -10 regions from pR are included along with some flanking sequences. The cutoff for the 5' end of the regulatory region was placed just after the pM -10 region which promotes transcription of a gene upstream (thus the pM -10 region is not included). The cutoff for the 3' end of the regulatory region was placed immediately prior to the RBS.
mutable notes: Derived from STHK022N . Literature claims that there is no cross-talk between HK022 cI regulatory region, Lambda cI, 434 cI and P22 cI, but this has not been verified experimentally by the synthetic biology group at MIT.
mutable provenance: Bacteriophage HK022.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -35
sequence annotation: OR1
sequence annotation: -10
sequence annotation: putative
sequence annotation: OR2
sequence annotation: BBa_R0050
sequence: https://synbiohub.org/public/igem/BBa_R0050_sequence/1

-35
type: SequenceAnnotation
persistent identity: annotation2012
display id: annotation2012
version: 1
top level: cI HK022
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0050/annotation2012/range2012/1

https://synbiohub.org/public/igem/BBa_R0050/annotation2012/range2012/1
type: Range
persistent identity: range2012
display id: range2012
version: 1
direction: direction/forward
top level: cI HK022
start: 8
end: 13
orientation: inline

OR1
type: SequenceAnnotation
persistent identity: annotation2015
display id: annotation2015
version: 1
top level: cI HK022
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_R0050/annotation2015/range2015/1

https://synbiohub.org/public/igem/BBa_R0050/annotation2015/range2015/1
type: Range
persistent identity: range2015
display id: range2015
version: 1
direction: direction/forward
top level: cI HK022
start: 37
end: 51
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2014
display id: annotation2014
version: 1
top level: cI HK022
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0050/annotation2014/range2014/1

https://synbiohub.org/public/igem/BBa_R0050/annotation2014/range2014/1
type: Range
persistent identity: range2014
display id: range2014
version: 1
direction: direction/forward
top level: cI HK022
start: 30
end: 35
orientation: inline

putative
type: SequenceAnnotation
persistent identity: annotation2018
display id: annotation2018
version: 1
top level: cI HK022
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_R0050/annotation2018/range2018/1

https://synbiohub.org/public/igem/BBa_R0050/annotation2018/range2018/1
type: Range
persistent identity: range2018
display id: range2018
version: 1
direction: direction/forward
top level: cI HK022
start: 43
end: 43
orientation: inline

OR2
type: SequenceAnnotation
persistent identity: annotation2013
display id: annotation2013
version: 1
top level: cI HK022
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_R0050/annotation2013/range2013/1

https://synbiohub.org/public/igem/BBa_R0050/annotation2013/range2013/1
type: Range
persistent identity: range2013
display id: range2013
version: 1
direction: direction/forward
top level: cI HK022
start: 13
end: 27
orientation: inline

BBa_R0050
type: SequenceAnnotation
persistent identity: annotation7066
display id: annotation7066
version: 1
top level: cI HK022
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_R0050/annotation7066/range7066/1

https://synbiohub.org/public/igem/BBa_R0050/annotation7066/range7066/1
type: Range
persistent identity: range7066
display id: range7066
version: 1
direction: direction/forward
top level: cI HK022
start: 1
end: 55
orientation: inline

CP1LacPI2
type: ComponentDefinition
persistent identity: BBa K418002
display id: BBa_K418002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: IPTG inducible Lac promoter cassette
creator: Northwestern University iGEM 2010
created: 2010-10-15T11:00:00Z
modified: 2015-05-08T01:12:28Z
discontinued: false
dominant: false
experience: experience/None
group u list: _532_
m user id: 0
owner id: 6938
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_J23100
component: BBa_Q04121
sequence annotation: BBa_Q04121
sequence annotation: BBa_J23100
sequence: https://synbiohub.org/public/igem/BBa_K418002_sequence/1

BBa_J23100
type: Component
persistent identity: component2263743
display id: component2263743
version: 1
top level: CP1LacPI2
access: public
definition: BBa_J23100

BBa_Q04121
type: Component
persistent identity: component2263762
display id: component2263762
version: 1
top level: CP1LacPI2
access: public
definition: https://synbiohub.org/public/igem/BBa_Q04121/1

BBa_Q04121
type: SequenceAnnotation
persistent identity: annotation2263762
display id: annotation2263762
version: 1
top level: CP1LacPI2
location: https://synbiohub.org/public/igem/BBa_K418002/annotation2263762/range2263762/1
component: BBa_Q04121

https://synbiohub.org/public/igem/BBa_K418002/annotation2263762/range2263762/1
type: Range
persistent identity: range2263762
display id: range2263762
version: 1
direction: direction/reverse
top level: CP1LacPI2
start: 44
end: 1414
orientation: inline

BBa_J23100
type: SequenceAnnotation
persistent identity: annotation2263743
display id: annotation2263743
version: 1
top level: CP1LacPI2
location: https://synbiohub.org/public/igem/BBa_K418002/annotation2263743/range2263743/1
component: BBa_J23100

https://synbiohub.org/public/igem/BBa_K418002/annotation2263743/range2263743/1
type: Range
persistent identity: range2263743
display id: range2263743
version: 1
direction: direction/reverse
top level: CP1LacPI2
start: 1
end: 35
orientation: inline

BBa_J64000
type: ComponentDefinition
persistent identity: BBa J64000
display id: BBa_J64000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: rhlI promoter
creator: Jeffrey J Tabor
created: 2007-01-15T12:00:00Z
modified: 2015-08-31T01:56:26Z
discontinued: false
dominant: false
experience: experience/None
group u list: _98_
m user id: 0
owner id: 88
owning group id: 98
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Promoter driving the rhlI gene in Pseudomonas aeruginosa. Activated by the RhlR+C4HSL. Also likely activated by LasR+3OC12HSL
mutable notes: none
mutable provenance: Genomic sequence of Pseudomonas aeruginosa
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: Rhl box
sequence: https://synbiohub.org/public/igem/BBa_J64000_sequence/1

Rhl box
type: SequenceAnnotation
persistent identity: annotation1910614
display id: annotation1910614
version: 1
top level: BBa_J64000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J64000/annotation1910614/range1910614/1

https://synbiohub.org/public/igem/BBa_J64000/annotation1910614/range1910614/1
type: Range
persistent identity: range1910614
display id: range1910614
version: 1
direction: direction/forward
top level: BBa_J64000
start: 11
end: 30
orientation: inline

BBa_M13106
type: ComponentDefinition
persistent identity: BBa M13106
display id: BBa_M13106
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: M13K07 gene VI promoter
creator: Natalie Kuldell
created: 2006-12-15T12:00:00Z
modified: 2015-05-08T01:13:55Z
discontinued: false
dominant: false
group u list: _45_
m user id: 0
owner id: 314
owning group id: 1
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This part is from the bacteriophage M13 genome, bp 2716-2764 in M13K07. It directs transcription of M13 gene VI (BBa_M13506, BBa_M13006). Its identity as a promoter was described in Gene (1980) 11:129-148 based on -10,-35 homology and fragment of genome able to bind RNAP.
mutable notes: none
mutable provenance: New England Biolabs M13K07. Sequenced in 2002 by L. Panganaban.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_M13106_sequence/1

RhlR+C4
type: ComponentDefinition
persistent identity: BBa R0071
display id: BBa_R0071
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter (RhlR & C4-HSL regulated)
creator: Ronny Krashinsky
created: 2004-01-24T12:00:00Z
modified: 2015-05-08T01:14:15Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Promoter activated by RhlR in concert with C4-HSL. C4-HSL is produced by RhlI, and acts as a quorum-sensing autoinducer. This is the natural sequence taken from Pseudomonas aeruginosa. Crosstalk: this promoter is also activated at a low level by LasR with its associated HSL.
mutable notes: The -10 and -35 sites are labeled as identified in [Pearson97]. The part begins with the RhlR binding site, and (rather arbitrarily) ends with the first transcribed base (+1).
mutable provenance: Pseudomonas aeruginosa rhlAB promoter
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -10
sequence annotation: start
sequence annotation: RhlR
sequence annotation: -35
sequence: https://synbiohub.org/public/igem/BBa_R0071_sequence/1

-10
type: SequenceAnnotation
persistent identity: annotation297104
display id: annotation297104
version: 1
top level: RhlR+C4
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0071/annotation297104/range297104/1

https://synbiohub.org/public/igem/BBa_R0071/annotation297104/range297104/1
type: Range
persistent identity: range297104
display id: range297104
version: 1
direction: direction/forward
top level: RhlR+C4
start: 42
end: 47
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation297105
display id: annotation297105
version: 1
top level: RhlR+C4
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_R0071/annotation297105/range297105/1

https://synbiohub.org/public/igem/BBa_R0071/annotation297105/range297105/1
type: Range
persistent identity: range297105
display id: range297105
version: 1
direction: direction/forward
top level: RhlR+C4
start: 53
end: 53
orientation: inline

RhlR
type: SequenceAnnotation
persistent identity: annotation297102
display id: annotation297102
version: 1
top level: RhlR+C4
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0071/annotation297102/range297102/1

https://synbiohub.org/public/igem/BBa_R0071/annotation297102/range297102/1
type: Range
persistent identity: range297102
display id: range297102
version: 1
direction: direction/forward
top level: RhlR+C4
start: 1
end: 20
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation297103
display id: annotation297103
version: 1
top level: RhlR+C4
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0071/annotation297103/range297103/1

https://synbiohub.org/public/igem/BBa_R0071/annotation297103/range297103/1
type: Range
persistent identity: range297103
display id: range297103
version: 1
direction: direction/forward
top level: RhlR+C4
start: 19
end: 24
orientation: inline

Pmra
type: ComponentDefinition
persistent identity: BBa S03331
display id: BBa_S03331
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: --Specify Parts List--
creator: Simone Macmil
created: 2005-09-01T11:00:00Z
modified: 2015-05-08T01:14:21Z
discontinued: false
dominant: false
group u list: _39_
m user id: 0
owner id: 318
owning group id: 39
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Promoter for cell division genes ftsI and ftsW
mutable notes: BBa_S03331is based on the reguatory region preceding cell division genes in the mra cluster and includes the biobrick head and tail restriction sites.
mutable provenance: A promoter for the first nine genes of the Escherichia coli mra cluster of cell division and cell envelope biosynthesis genes, including ftsI and ftsW. Hara H, Yasuda S, Horiuchi K and Park JT. Journal of Bacteriology (1997) 179: 5802-5811
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_S03331_sequence/1

BBa_K266003
type: ComponentDefinition
persistent identity: BBa K266003
display id: BBa_K266003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: POPS -> Lac Inverter -> LasR
creator: UNAM FCIENCIAS-CINVESTAV IRA
created: 2009-10-12T11:00:00Z
modified: 2015-05-08T01:11:43Z
discontinued: false
dominant: false
experience: experience/None
group u list: _363_
m user id: 0
owner id: 1955
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Lac system inverter controlling the expression of LasR
mutable notes: None
mutable provenance: The biobricks BBa_Q04121, BBa_B0034 and BBa_K266002 where used to build this biobrick
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Inverter
component: BBa_K266002
component: BBa_B0034
component: BBa_Q04121
sequence annotation: BBa_B0034
sequence annotation: BBa_K266002
sequence annotation: BBa_Q04121
sequence: https://synbiohub.org/public/igem/BBa_K266003_sequence/1

BBa_K266002
type: Component
persistent identity: component2276870
display id: component2276870
version: 1
top level: BBa_K266003
access: public
definition: https://synbiohub.org/public/igem/BBa_K266002/1

BBa_B0034
type: Component
persistent identity: component2276857
display id: component2276857
version: 1
top level: BBa_K266003
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_Q04121
type: Component
persistent identity: component2276855
display id: component2276855
version: 1
top level: BBa_K266003
access: public
definition: https://synbiohub.org/public/igem/BBa_Q04121/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2276857
display id: annotation2276857
version: 1
top level: BBa_K266003
location: https://synbiohub.org/public/igem/BBa_K266003/annotation2276857/range2276857/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K266003/annotation2276857/range2276857/1
type: Range
persistent identity: range2276857
display id: range2276857
version: 1
direction: direction/reverse
top level: BBa_K266003
start: 1380
end: 1391
orientation: inline

BBa_K266002
type: SequenceAnnotation
persistent identity: annotation2276870
display id: annotation2276870
version: 1
top level: BBa_K266003
location: https://synbiohub.org/public/igem/BBa_K266003/annotation2276870/range2276870/1
component: BBa_K266002

https://synbiohub.org/public/igem/BBa_K266003/annotation2276870/range2276870/1
type: Range
persistent identity: range2276870
display id: range2276870
version: 1
direction: direction/reverse
top level: BBa_K266003
start: 1398
end: 2315
orientation: inline

BBa_Q04121
type: SequenceAnnotation
persistent identity: annotation2276855
display id: annotation2276855
version: 1
top level: BBa_K266003
location: https://synbiohub.org/public/igem/BBa_K266003/annotation2276855/range2276855/1
component: BBa_Q04121

https://synbiohub.org/public/igem/BBa_K266003/annotation2276855/range2276855/1
type: Range
persistent identity: range2276855
display id: range2276855
version: 1
direction: direction/reverse
top level: BBa_K266003
start: 1
end: 1371
orientation: inline

U037O11+D0
type: ComponentDefinition
persistent identity: BBa I732448
display id: BBa_I732448
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member (U037O11+D062O22)
creator: Zhan Jian
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732448_sequence/1

BBa_R2109
type: ComponentDefinition
persistent identity: BBa R2109
display id: BBa_R2109
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter with operator site for C2003
creator: Reshma Shetty
created: 2006-10-20T11:00:00Z
modified: 2015-05-08T01:14:16Z
discontinued: false
dominant: false
group u list: _41_
m user id: 0
owner id: 126
owning group id: 70
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This promoter is designed to bind to C2003.
mutable notes: Placement of the operator site was determined to optimize steric interference between C2003 and RNA polymerase.
mutable provenance: This promoter was rationally designed and synthesized.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -35 site
sequence annotation: -10 site
sequence annotation: transcription start site
sequence annotation: operator site for C2003
sequence: https://synbiohub.org/public/igem/BBa_R2109_sequence/1

-35 site
type: SequenceAnnotation
persistent identity: annotation1903912
display id: annotation1903912
version: 1
top level: BBa_R2109
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R2109/annotation1903912/range1903912/1

https://synbiohub.org/public/igem/BBa_R2109/annotation1903912/range1903912/1
type: Range
persistent identity: range1903912
display id: range1903912
version: 1
direction: direction/forward
top level: BBa_R2109
start: 17
end: 22
orientation: inline

-10 site
type: SequenceAnnotation
persistent identity: annotation1903913
display id: annotation1903913
version: 1
top level: BBa_R2109
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R2109/annotation1903913/range1903913/1

https://synbiohub.org/public/igem/BBa_R2109/annotation1903913/range1903913/1
type: Range
persistent identity: range1903913
display id: range1903913
version: 1
direction: direction/forward
top level: BBa_R2109
start: 40
end: 45
orientation: inline

transcription start site
type: SequenceAnnotation
persistent identity: annotation1903914
display id: annotation1903914
version: 1
top level: BBa_R2109
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_R2109/annotation1903914/range1903914/1

https://synbiohub.org/public/igem/BBa_R2109/annotation1903914/range1903914/1
type: Range
persistent identity: range1903914
display id: range1903914
version: 1
direction: direction/forward
top level: BBa_R2109
start: 53
end: 53
orientation: inline

operator site for C2003
type: SequenceAnnotation
persistent identity: annotation1903915
display id: annotation1903915
version: 1
top level: BBa_R2109
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_R2109/annotation1903915/range1903915/1

https://synbiohub.org/public/igem/BBa_R2109/annotation1903915/range1903915/1
type: Range
persistent identity: range1903915
display id: range1903915
version: 1
direction: direction/forward
top level: BBa_R2109
start: 22
end: 33
orientation: inline

U061O11+D0
type: ComponentDefinition
persistent identity: BBa I732404
display id: BBa_I732404
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member (U061O11+D062NUL)
creator: Zhan Jian
created: 2007-09-19T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1572
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: A Double Reporter (LacZ-alpha and GFP-AAV) loaded on pSB3K5. For more information please refer to BBa_I732401.
mutable notes: Might be later edited.
mutable provenance: Might be later edited.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: T7 TE
sequence annotation: BBa_B0034
sequence annotation: BBa_I732006
sequence annotation: lacZ
sequence annotation: conserved
sequence annotation: BBa_B0012
sequence annotation: START
sequence annotation: BBa_B0010
sequence annotation: BamHI
sequence annotation: stem_loop
sequence annotation: BglII
sequence annotation: STOP
sequence annotation: stop
sequence annotation: BBa_E0044
sequence annotation: polya
sequence: https://synbiohub.org/public/igem/BBa_I732404_sequence/1

T7 TE
type: SequenceAnnotation
persistent identity: annotation1947698
display id: annotation1947698
version: 1
top level: U061O11+D0
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_I732404/annotation1947698/range1947698/1

https://synbiohub.org/public/igem/BBa_I732404/annotation1947698/range1947698/1
type: Range
persistent identity: range1947698
display id: range1947698
version: 1
direction: direction/forward
top level: U061O11+D0
start: 1168
end: 1187
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1947686
display id: annotation1947686
version: 1
top level: U061O11+D0
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I732404/annotation1947686/range1947686/1

https://synbiohub.org/public/igem/BBa_I732404/annotation1947686/range1947686/1
type: Range
persistent identity: range1947686
display id: range1947686
version: 1
direction: direction/reverse
top level: U061O11+D0
start: 28
end: 39
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1947692
display id: annotation1947692
version: 1
top level: U061O11+D0
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I732404/annotation1947692/range1947692/1

https://synbiohub.org/public/igem/BBa_I732404/annotation1947692/range1947692/1
type: Range
persistent identity: range1947692
display id: range1947692
version: 1
direction: direction/reverse
top level: U061O11+D0
start: 288
end: 299
orientation: inline

BBa_I732006
type: SequenceAnnotation
persistent identity: annotation1947688
display id: annotation1947688
version: 1
top level: U061O11+D0
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I732404/annotation1947688/range1947688/1

https://synbiohub.org/public/igem/BBa_I732404/annotation1947688/range1947688/1
type: Range
persistent identity: range1947688
display id: range1947688
version: 1
direction: direction/reverse
top level: U061O11+D0
start: 46
end: 279
orientation: inline

lacZ
type: SequenceAnnotation
persistent identity: annotation1947689
display id: annotation1947689
version: 1
top level: U061O11+D0
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_I732404/annotation1947689/range1947689/1

https://synbiohub.org/public/igem/BBa_I732404/annotation1947689/range1947689/1
type: Range
persistent identity: range1947689
display id: range1947689
version: 1
direction: direction/forward
top level: U061O11+D0
start: 46
end: 279
orientation: inline

conserved
type: SequenceAnnotation
persistent identity: annotation1947687
display id: annotation1947687
version: 1
top level: U061O11+D0
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_I732404/annotation1947687/range1947687/1

https://synbiohub.org/public/igem/BBa_I732404/annotation1947687/range1947687/1
type: Range
persistent identity: range1947687
display id: range1947687
version: 1
direction: direction/forward
top level: U061O11+D0
start: 32
end: 35
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1947697
display id: annotation1947697
version: 1
top level: U061O11+D0
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I732404/annotation1947697/range1947697/1

https://synbiohub.org/public/igem/BBa_I732404/annotation1947697/range1947697/1
type: Range
persistent identity: range1947697
display id: range1947697
version: 1
direction: direction/forward
top level: U061O11+D0
start: 1161
end: 1201
orientation: inline

START
type: SequenceAnnotation
persistent identity: annotation1947690
display id: annotation1947690
version: 1
top level: U061O11+D0
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_I732404/annotation1947690/range1947690/1

https://synbiohub.org/public/igem/BBa_I732404/annotation1947690/range1947690/1
type: Range
persistent identity: range1947690
display id: range1947690
version: 1
direction: direction/forward
top level: U061O11+D0
start: 46
end: 48
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1947695
display id: annotation1947695
version: 1
top level: U061O11+D0
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I732404/annotation1947695/range1947695/1

https://synbiohub.org/public/igem/BBa_I732404/annotation1947695/range1947695/1
type: Range
persistent identity: range1947695
display id: range1947695
version: 1
direction: direction/forward
top level: U061O11+D0
start: 1073
end: 1152
orientation: inline

BamHI
type: SequenceAnnotation
persistent identity: annotation1947685
display id: annotation1947685
version: 1
top level: U061O11+D0
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I732404/annotation1947685/range1947685/1

https://synbiohub.org/public/igem/BBa_I732404/annotation1947685/range1947685/1
type: Range
persistent identity: range1947685
display id: range1947685
version: 1
direction: direction/forward
top level: U061O11+D0
start: 12
end: 17
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation1947696
display id: annotation1947696
version: 1
top level: U061O11+D0
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_I732404/annotation1947696/range1947696/1

https://synbiohub.org/public/igem/BBa_I732404/annotation1947696/range1947696/1
type: Range
persistent identity: range1947696
display id: range1947696
version: 1
direction: direction/forward
top level: U061O11+D0
start: 1084
end: 1127
orientation: inline

BglII
type: SequenceAnnotation
persistent identity: annotation1947684
display id: annotation1947684
version: 1
top level: U061O11+D0
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I732404/annotation1947684/range1947684/1

https://synbiohub.org/public/igem/BBa_I732404/annotation1947684/range1947684/1
type: Range
persistent identity: range1947684
display id: range1947684
version: 1
direction: direction/forward
top level: U061O11+D0
start: 3
end: 8
orientation: inline

conserved
type: SequenceAnnotation
persistent identity: annotation1947693
display id: annotation1947693
version: 1
top level: U061O11+D0
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_I732404/annotation1947693/range1947693/1

https://synbiohub.org/public/igem/BBa_I732404/annotation1947693/range1947693/1
type: Range
persistent identity: range1947693
display id: range1947693
version: 1
direction: direction/forward
top level: U061O11+D0
start: 292
end: 295
orientation: inline

STOP
type: SequenceAnnotation
persistent identity: annotation1947691
display id: annotation1947691
version: 1
top level: U061O11+D0
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_I732404/annotation1947691/range1947691/1

https://synbiohub.org/public/igem/BBa_I732404/annotation1947691/range1947691/1
type: Range
persistent identity: range1947691
display id: range1947691
version: 1
direction: direction/forward
top level: U061O11+D0
start: 274
end: 279
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1947700
display id: annotation1947700
version: 1
top level: U061O11+D0
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_I732404/annotation1947700/range1947700/1

https://synbiohub.org/public/igem/BBa_I732404/annotation1947700/range1947700/1
type: Range
persistent identity: range1947700
display id: range1947700
version: 1
direction: direction/forward
top level: U061O11+D0
start: 1194
end: 1194
orientation: inline

BBa_E0044
type: SequenceAnnotation
persistent identity: annotation1947694
display id: annotation1947694
version: 1
top level: U061O11+D0
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I732404/annotation1947694/range1947694/1

https://synbiohub.org/public/igem/BBa_I732404/annotation1947694/range1947694/1
type: Range
persistent identity: range1947694
display id: range1947694
version: 1
direction: direction/reverse
top level: U061O11+D0
start: 306
end: 1064
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1947699
display id: annotation1947699
version: 1
top level: U061O11+D0
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_I732404/annotation1947699/range1947699/1

https://synbiohub.org/public/igem/BBa_I732404/annotation1947699/range1947699/1
type: Range
persistent identity: range1947699
display id: range1947699
version: 1
direction: direction/forward
top level: U061O11+D0
start: 1188
end: 1201
orientation: inline

BBa_I739101
type: ComponentDefinition
persistent identity: BBa I739101
display id: BBa_I739101
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Double Promoter (constitutive / TetR, negative)
creator: Stefan Luzi
created: 2007-10-11T11:00:00Z
modified: 2015-08-31T04:08:01Z
discontinued: false
dominant: false
experience: experience/None
group u list: _124_
m user id: 0
owner id: 2183
owning group id: 62
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: sdfasdf
mutable notes: asdf
mutable provenance: Synthetic DNA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: TetR 1
sequence annotation: J23100
sequence annotation: Scar
sequence annotation: R0040 (2x TetR 1)
sequence: https://synbiohub.org/public/igem/BBa_I739101_sequence/1

TetR 1
type: SequenceAnnotation
persistent identity: annotation1946101
display id: annotation1946101
version: 1
top level: BBa_I739101
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I739101/annotation1946101/range1946101/1

https://synbiohub.org/public/igem/BBa_I739101/annotation1946101/range1946101/1
type: Range
persistent identity: range1946101
display id: range1946101
version: 1
direction: direction/forward
top level: BBa_I739101
start: 64
end: 83
orientation: inline

J23100
type: SequenceAnnotation
persistent identity: annotation1946100
display id: annotation1946100
version: 1
top level: BBa_I739101
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I739101/annotation1946100/range1946100/1

https://synbiohub.org/public/igem/BBa_I739101/annotation1946100/range1946100/1
type: Range
persistent identity: range1946100
display id: range1946100
version: 1
direction: direction/forward
top level: BBa_I739101
start: 1
end: 35
orientation: inline

Scar
type: SequenceAnnotation
persistent identity: annotation1946099
display id: annotation1946099
version: 1
top level: BBa_I739101
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I739101/annotation1946099/range1946099/1

https://synbiohub.org/public/igem/BBa_I739101/annotation1946099/range1946099/1
type: Range
persistent identity: range1946099
display id: range1946099
version: 1
direction: direction/forward
top level: BBa_I739101
start: 36
end: 43
orientation: inline

TetR 1
type: SequenceAnnotation
persistent identity: annotation1946102
display id: annotation1946102
version: 1
top level: BBa_I739101
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I739101/annotation1946102/range1946102/1

https://synbiohub.org/public/igem/BBa_I739101/annotation1946102/range1946102/1
type: Range
persistent identity: range1946102
display id: range1946102
version: 1
direction: direction/forward
top level: BBa_I739101
start: 44
end: 63
orientation: inline

R0040 (2x TetR 1)
type: SequenceAnnotation
persistent identity: annotation1946257
display id: annotation1946257
version: 1
top level: BBa_I739101
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_I739101/annotation1946257/range1946257/1

https://synbiohub.org/public/igem/BBa_I739101/annotation1946257/range1946257/1
type: Range
persistent identity: range1946257
display id: range1946257
version: 1
direction: direction/forward
top level: BBa_I739101
start: 44
end: 83
orientation: inline

U073O11+D0
type: ComponentDefinition
persistent identity: BBa I732427
display id: BBa_I732427
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member (U073O11+D026O22)
creator: Zhan Jian
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732427_sequence/1

CP2LacPI1
type: ComponentDefinition
persistent identity: BBa K418003
display id: BBa_K418003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: IPTG inducible Lac promoter cassette
creator: Northwestern University iGEM 2010
created: 2010-10-15T11:00:00Z
modified: 2015-05-08T01:12:28Z
discontinued: false
dominant: false
experience: experience/None
group u list: _532_
m user id: 0
owner id: 6938
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_Q01121
component: BBa_J23104
sequence annotation: BBa_Q01121
sequence annotation: BBa_J23104
sequence: https://synbiohub.org/public/igem/BBa_K418003_sequence/1

BBa_Q01121
type: Component
persistent identity: component2263782
display id: component2263782
version: 1
top level: CP2LacPI1
access: public
definition: https://synbiohub.org/public/igem/BBa_Q01121/1

BBa_J23104
type: Component
persistent identity: component2263763
display id: component2263763
version: 1
top level: CP2LacPI1
access: public
definition: BBa_J23104

BBa_Q01121
type: SequenceAnnotation
persistent identity: annotation2263782
display id: annotation2263782
version: 1
top level: CP2LacPI1
location: https://synbiohub.org/public/igem/BBa_K418003/annotation2263782/range2263782/1
component: BBa_Q01121

https://synbiohub.org/public/igem/BBa_K418003/annotation2263782/range2263782/1
type: Range
persistent identity: range2263782
display id: range2263782
version: 1
direction: direction/reverse
top level: CP2LacPI1
start: 44
end: 1416
orientation: inline

BBa_J23104
type: SequenceAnnotation
persistent identity: annotation2263763
display id: annotation2263763
version: 1
top level: CP2LacPI1
location: https://synbiohub.org/public/igem/BBa_K418003/annotation2263763/range2263763/1
component: BBa_J23104

https://synbiohub.org/public/igem/BBa_K418003/annotation2263763/range2263763/1
type: Range
persistent identity: range2263763
display id: range2263763
version: 1
direction: direction/reverse
top level: CP2LacPI1
start: 1
end: 35
orientation: inline

U097NUL+D0
type: ComponentDefinition
persistent identity: BBa I732411
display id: BBa_I732411
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member (U097NUL+D050O22)
creator: Zhan Jian
created: 2007-09-22T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1572
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: To be later edited if time permits.
mutable notes: To be later edited if time permits.
mutable provenance: To be later edited if time permits.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: consensus elements
sequence annotation: O2
sequence annotation: +1
sequence: https://synbiohub.org/public/igem/BBa_I732411_sequence/1

consensus elements
type: SequenceAnnotation
persistent identity: annotation1944561
display id: annotation1944561
version: 1
top level: U097NUL+D0
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I732411/annotation1944561/range1944561/1

https://synbiohub.org/public/igem/BBa_I732411/annotation1944561/range1944561/1
type: Range
persistent identity: range1944561
display id: range1944561
version: 1
direction: direction/forward
top level: U097NUL+D0
start: 61
end: 137
orientation: inline

O2
type: SequenceAnnotation
persistent identity: annotation1944562
display id: annotation1944562
version: 1
top level: U097NUL+D0
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_I732411/annotation1944562/range1944562/1

https://synbiohub.org/public/igem/BBa_I732411/annotation1944562/range1944562/1
type: Range
persistent identity: range1944562
display id: range1944562
version: 1
direction: direction/forward
top level: U097NUL+D0
start: 146
end: 165
orientation: inline

+1
type: SequenceAnnotation
persistent identity: annotation1944563
display id: annotation1944563
version: 1
top level: U097NUL+D0
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_I732411/annotation1944563/range1944563/1

https://synbiohub.org/public/igem/BBa_I732411/annotation1944563/range1944563/1
type: Range
persistent identity: range1944563
display id: range1944563
version: 1
direction: direction/forward
top level: U097NUL+D0
start: 137
end: 137
orientation: inline

BBa_J07007
type: ComponentDefinition
persistent identity: BBa J07007
display id: BBa_J07007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: ctx promoter
creator: igem_mit_2005
created: 2005-07-05T11:00:00Z
modified: 2015-08-31T04:08:19Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_12_21_1_
m user id: 0
owner id: 363
owning group id: 21
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: V.cholerae ctx gene promoter region. Transcription takes place In the presence of a ToxR dimer. We don't have the -10 and -35 sites annotated (need to do this).
mutable provenance: GENBANK M21250
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: ctx promoter
sequence annotation: transcription start
sequence annotation: -10 region
sequence: https://synbiohub.org/public/igem/BBa_J07007_sequence/1

ctx promoter
type: SequenceAnnotation
persistent identity: annotation1571612
display id: annotation1571612
version: 1
top level: BBa_J07007
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J07007/annotation1571612/range1571612/1

https://synbiohub.org/public/igem/BBa_J07007/annotation1571612/range1571612/1
type: Range
persistent identity: range1571612
display id: range1571612
version: 1
direction: direction/forward
top level: BBa_J07007
start: 1
end: 145
orientation: inline

transcription start
type: SequenceAnnotation
persistent identity: annotation1577387
display id: annotation1577387
version: 1
top level: BBa_J07007
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J07007/annotation1577387/range1577387/1

https://synbiohub.org/public/igem/BBa_J07007/annotation1577387/range1577387/1
type: Range
persistent identity: range1577387
display id: range1577387
version: 1
direction: direction/forward
top level: BBa_J07007
start: 145
end: 145
orientation: inline

-10 region
type: SequenceAnnotation
persistent identity: annotation1579234
display id: annotation1579234
version: 1
top level: BBa_J07007
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J07007/annotation1579234/range1579234/1

https://synbiohub.org/public/igem/BBa_J07007/annotation1579234/range1579234/1
type: Range
persistent identity: range1579234
display id: range1579234
version: 1
direction: direction/forward
top level: BBa_J07007
start: 133
end: 138
orientation: inline

Pnot(O1wt1
type: ComponentDefinition
persistent identity: BBa I732200
display id: BBa_I732200
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: NOT Gate Promoter Family Member (D001O1wt1)
creator: Zhan Jian
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:57Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732200_sequence/1

BBa_J31013
type: ComponentDefinition
persistent identity: BBa J31013
display id: BBa_J31013
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pLac Backwards [cf. BBa_R0010]
creator: Karmella Haynes
created: 2007-04-11T11:00:00Z
modified: 2015-08-31T04:08:45Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _61_
m user id: 0
owner id: 1144
owning group id: 61
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Promoter from the lack operon cloned in the reverse orientation.
mutable notes: Promoter from the lack operon cloned in the reverse orientation.
mutable provenance: BBa_R0010
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -10
sequence annotation: end of LacI coding region (inactive)
sequence annotation: R0010 reverse
sequence annotation: LacI binding site
sequence annotation: -35
sequence annotation: CAP binding site
sequence: https://synbiohub.org/public/igem/BBa_J31013_sequence/1

-10
type: SequenceAnnotation
persistent identity: annotation1928672
display id: annotation1928672
version: 1
top level: BBa_J31013
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J31013/annotation1928672/range1928672/1

https://synbiohub.org/public/igem/BBa_J31013/annotation1928672/range1928672/1
type: Range
persistent identity: range1928672
display id: range1928672
version: 1
direction: direction/reverse
top level: BBa_J31013
start: 35
end: 40
orientation: inline

end of LacI coding region (inactive)
type: SequenceAnnotation
persistent identity: annotation1928669
display id: annotation1928669
version: 1
top level: BBa_J31013
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J31013/annotation1928669/range1928669/1

https://synbiohub.org/public/igem/BBa_J31013/annotation1928669/range1928669/1
type: Range
persistent identity: range1928669
display id: range1928669
version: 1
direction: direction/reverse
top level: BBa_J31013
start: 113
end: 200
orientation: inline

R0010 reverse
type: SequenceAnnotation
persistent identity: annotation1928668
display id: annotation1928668
version: 1
top level: BBa_J31013
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_J31013/annotation1928668/range1928668/1

https://synbiohub.org/public/igem/BBa_J31013/annotation1928668/range1928668/1
type: Range
persistent identity: range1928668
display id: range1928668
version: 1
direction: direction/reverse
top level: BBa_J31013
start: 1
end: 200
orientation: inline

LacI binding site
type: SequenceAnnotation
persistent identity: annotation1928673
display id: annotation1928673
version: 1
top level: BBa_J31013
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_J31013/annotation1928673/range1928673/1

https://synbiohub.org/public/igem/BBa_J31013/annotation1928673/range1928673/1
type: Range
persistent identity: range1928673
display id: range1928673
version: 1
direction: direction/forward
top level: BBa_J31013
start: 1
end: 34
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1928671
display id: annotation1928671
version: 1
top level: BBa_J31013
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J31013/annotation1928671/range1928671/1

https://synbiohub.org/public/igem/BBa_J31013/annotation1928671/range1928671/1
type: Range
persistent identity: range1928671
display id: range1928671
version: 1
direction: direction/reverse
top level: BBa_J31013
start: 58
end: 64
orientation: inline

CAP binding site
type: SequenceAnnotation
persistent identity: annotation1928670
display id: annotation1928670
version: 1
top level: BBa_J31013
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_J31013/annotation1928670/range1928670/1

https://synbiohub.org/public/igem/BBa_J31013/annotation1928670/range1928670/1
type: Range
persistent identity: range1928670
display id: range1928670
version: 1
direction: direction/forward
top level: BBa_J31013
start: 74
end: 112
orientation: inline

BBa_K180018
type: ComponentDefinition
persistent identity: BBa K180018
display id: BBa_K180018
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Scissors - Primary plasmid (part 1) [LasR generator]
creator: Richard Wilson, Stephanie Chambers
created: 2009-10-18T11:00:00Z
modified: 2015-05-08T01:11:06Z
discontinued: false
dominant: false
experience: experience/None
group u list: _278_
m user id: 0
owner id: 4082
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Part of the iGEM2009 Team:Southampton rock-paper-scissors 'scissors' primary plasmid.
mutable notes: Constitutive promoter selected to allow for continuous expression of C0179.
mutable provenance: See relevant basic biobrick parts (J23119, B0034, C0179, B0015)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0012
component: BBa_B0010
component: BBa_C0179
component: BBa_J23119
component: BBa_B0034
sequence annotation: BBa_B0010
sequence annotation: BBa_J23119
sequence annotation: BBa_B0012
sequence annotation: BBa_B0034
sequence annotation: BBa_C0179
sequence: https://synbiohub.org/public/igem/BBa_K180018_sequence/1

BBa_B0012
type: Component
persistent identity: component2053918
display id: component2053918
version: 1
top level: BBa_K180018
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0010
type: Component
persistent identity: component2053916
display id: component2053916
version: 1
top level: BBa_K180018
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_C0179
type: Component
persistent identity: component2053915
display id: component2053915
version: 1
top level: BBa_K180018
access: public
definition: https://synbiohub.org/public/igem/BBa_C0179/1

BBa_J23119
type: Component
persistent identity: component2053912
display id: component2053912
version: 1
top level: BBa_K180018
access: public
definition: BBa_J23119

BBa_B0034
type: Component
persistent identity: component2053914
display id: component2053914
version: 1
top level: BBa_K180018
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2053916
display id: annotation2053916
version: 1
top level: BBa_K180018
location: https://synbiohub.org/public/igem/BBa_K180018/annotation2053916/range2053916/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K180018/annotation2053916/range2053916/1
type: Range
persistent identity: range2053916
display id: range2053916
version: 1
direction: direction/forward
top level: BBa_K180018
start: 793
end: 872
orientation: inline

BBa_J23119
type: SequenceAnnotation
persistent identity: annotation2053912
display id: annotation2053912
version: 1
top level: BBa_K180018
location: https://synbiohub.org/public/igem/BBa_K180018/annotation2053912/range2053912/1
component: BBa_J23119

https://synbiohub.org/public/igem/BBa_K180018/annotation2053912/range2053912/1
type: Range
persistent identity: range2053912
display id: range2053912
version: 1
direction: direction/reverse
top level: BBa_K180018
start: 1
end: 35
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2053918
display id: annotation2053918
version: 1
top level: BBa_K180018
location: https://synbiohub.org/public/igem/BBa_K180018/annotation2053918/range2053918/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K180018/annotation2053918/range2053918/1
type: Range
persistent identity: range2053918
display id: range2053918
version: 1
direction: direction/forward
top level: BBa_K180018
start: 881
end: 921
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2053914
display id: annotation2053914
version: 1
top level: BBa_K180018
location: https://synbiohub.org/public/igem/BBa_K180018/annotation2053914/range2053914/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K180018/annotation2053914/range2053914/1
type: Range
persistent identity: range2053914
display id: range2053914
version: 1
direction: direction/reverse
top level: BBa_K180018
start: 44
end: 55
orientation: inline

BBa_C0179
type: SequenceAnnotation
persistent identity: annotation2053915
display id: annotation2053915
version: 1
top level: BBa_K180018
location: https://synbiohub.org/public/igem/BBa_K180018/annotation2053915/range2053915/1
component: BBa_C0179

https://synbiohub.org/public/igem/BBa_K180018/annotation2053915/range2053915/1
type: Range
persistent identity: range2053915
display id: range2053915
version: 1
direction: direction/reverse
top level: BBa_K180018
start: 62
end: 784
orientation: inline

BBa_I746362
type: ComponentDefinition
persistent identity: BBa I746362
display id: BBa_I746362
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PP promoter from P2 phage
creator: Stefan Milde
created: 2007-09-10T11:00:00Z
modified: 2015-08-31T04:08:04Z
discontinued: false
dominant: false
experience: experience/None
group u list: _116_
m user id: 0
owner id: 2122
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This is the PP promoter taken from the P2 phage genome. It is an inducible promoter that is activated by a class of activators, including P2 ogr (I746350), PSP3 pag (I746351) and phiR73 delta (I746352). These different activators should cause different levels of activity of the PP promoter.
mutable notes: no special considerations
mutable provenance: P2 phage genome
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: PP
sequence: https://synbiohub.org/public/igem/BBa_I746362_sequence/1

PP
type: SequenceAnnotation
persistent identity: annotation1943785
display id: annotation1943785
version: 1
top level: BBa_I746362
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I746362/annotation1943785/range1943785/1

https://synbiohub.org/public/igem/BBa_I746362/annotation1943785/range1943785/1
type: Range
persistent identity: range1943785
display id: range1943785
version: 1
direction: direction/forward
top level: BBa_I746362
start: 1
end: 92
orientation: inline

NAND2717
type: ComponentDefinition
persistent identity: BBa I732302
display id: BBa_I732302
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: NAND Candidate (U073O27D001O17)
creator: Ma Rui
created: 2007-10-02T11:00:00Z
modified: 2015-08-31T04:07:57Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _156_
m user id: 0
owner id: 1592
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: U97NUL D001O22
mutable notes: .
mutable provenance: .
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732302_sequence/1

cI 434
type: ComponentDefinition
persistent identity: BBa R1052
display id: BBa_R1052
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter, Standard (434 cI regulated)
creator: Maia Mahoney, Drew Endy
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:15Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: [Note: This is the same part as R0052 except that the -10 and -35 sites and spacing have been changed to comply with BBa_S0001]. The 434 cI regulatory region sequence is a 89 base-pair sequence with the standard BioBrick prefix and suffix sections on its ends. 434 cI repressor protein, <bb_part>BBa_C0052<bb_part>, binds to it. This segment contains O-R1, O-R2, P-R, and P-RM -35.
mutable notes: Wild-type promoter modified to comply with BBa_S0001: TTGACA-17N-GATACT
mutable provenance: Bacteriophage 434 right operator
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -35
sequence annotation: BBa_R1052
sequence annotation: -10
sequence annotation: OR1
sequence annotation: OR2
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_R1052_sequence/1

-35
type: SequenceAnnotation
persistent identity: annotation2082
display id: annotation2082
version: 1
top level: cI 434
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R1052/annotation2082/range2082/1

https://synbiohub.org/public/igem/BBa_R1052/annotation2082/range2082/1
type: Range
persistent identity: range2082
display id: range2082
version: 1
direction: direction/forward
top level: cI 434
start: 1
end: 6
orientation: inline

BBa_R1052
type: SequenceAnnotation
persistent identity: annotation7075
display id: annotation7075
version: 1
top level: cI 434
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_R1052/annotation7075/range7075/1

https://synbiohub.org/public/igem/BBa_R1052/annotation7075/range7075/1
type: Range
persistent identity: range7075
display id: range7075
version: 1
direction: direction/forward
top level: cI 434
start: 1
end: 46
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2087
display id: annotation2087
version: 1
top level: cI 434
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R1052/annotation2087/range2087/1

https://synbiohub.org/public/igem/BBa_R1052/annotation2087/range2087/1
type: Range
persistent identity: range2087
display id: range2087
version: 1
direction: direction/forward
top level: cI 434
start: 24
end: 29
orientation: inline

OR1
type: SequenceAnnotation
persistent identity: annotation2081
display id: annotation2081
version: 1
top level: cI 434
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R1052/annotation2081/range2081/1

https://synbiohub.org/public/igem/BBa_R1052/annotation2081/range2081/1
type: Range
persistent identity: range2081
display id: range2081
version: 1
direction: direction/forward
top level: cI 434
start: 30
end: 43
orientation: inline

OR2
type: SequenceAnnotation
persistent identity: annotation2080
display id: annotation2080
version: 1
top level: cI 434
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R1052/annotation2080/range2080/1

https://synbiohub.org/public/igem/BBa_R1052/annotation2080/range2080/1
type: Range
persistent identity: range2080
display id: range2080
version: 1
direction: direction/forward
top level: cI 434
start: 8
end: 21
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2083
display id: annotation2083
version: 1
top level: cI 434
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R1052/annotation2083/range2083/1

https://synbiohub.org/public/igem/BBa_R1052/annotation2083/range2083/1
type: Range
persistent identity: range2083
display id: range2083
version: 1
direction: direction/forward
top level: cI 434
start: 2
end: 7
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2084
display id: annotation2084
version: 1
top level: cI 434
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_R1052/annotation2084/range2084/1

https://synbiohub.org/public/igem/BBa_R1052/annotation2084/range2084/1
type: Range
persistent identity: range2084
display id: range2084
version: 1
direction: direction/forward
top level: cI 434
start: 35
end: 37
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2079
display id: annotation2079
version: 1
top level: cI 434
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R1052/annotation2079/range2079/1

https://synbiohub.org/public/igem/BBa_R1052/annotation2079/range2079/1
type: Range
persistent identity: range2079
display id: range2079
version: 1
direction: direction/forward
top level: cI 434
start: 1
end: 43
orientation: inline

BBa_K180020
type: ComponentDefinition
persistent identity: BBa K180020
display id: BBa_K180020
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Rock-paper-scissors - Scissors secondary plasmid [las pR, LacI generator]
creator: Richard Wilson, Stephanie Chambers
created: 2009-10-18T11:00:00Z
modified: 2015-05-08T01:11:06Z
discontinued: false
dominant: false
experience: experience/None
group u list: _278_
m user id: 0
owner id: 4082
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: iGEM2009 Team:Southampton rock-paper-scissors 'scissors' secondary plasmid.
mutable notes: Regulated expression of LacI (C0012) by Las pR (R0079).
mutable provenance: See relevant basic biobrick parts (R0070, B0034, C0012, B0015).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0034
component: BBa_R0079
component: BBa_C0012
component: BBa_B0015
sequence annotation: BBa_B0034
sequence annotation: BBa_B0015
sequence annotation: BBa_R0079
sequence annotation: BBa_C0012
sequence: https://synbiohub.org/public/igem/BBa_K180020_sequence/1

BBa_B0034
type: Component
persistent identity: component2256458
display id: component2256458
version: 1
top level: BBa_K180020
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_R0079
type: Component
persistent identity: component2256456
display id: component2256456
version: 1
top level: BBa_K180020
access: public
definition: LasR+PAI

BBa_C0012
type: Component
persistent identity: component2256460
display id: component2256460
version: 1
top level: BBa_K180020
access: public
definition: https://synbiohub.org/public/igem/BBa_C0012/1

BBa_B0015
type: Component
persistent identity: component2256469
display id: component2256469
version: 1
top level: BBa_K180020
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2256458
display id: annotation2256458
version: 1
top level: BBa_K180020
location: https://synbiohub.org/public/igem/BBa_K180020/annotation2256458/range2256458/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K180020/annotation2256458/range2256458/1
type: Range
persistent identity: range2256458
display id: range2256458
version: 1
direction: direction/reverse
top level: BBa_K180020
start: 166
end: 177
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2256469
display id: annotation2256469
version: 1
top level: BBa_K180020
location: https://synbiohub.org/public/igem/BBa_K180020/annotation2256469/range2256469/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K180020/annotation2256469/range2256469/1
type: Range
persistent identity: range2256469
display id: range2256469
version: 1
direction: direction/reverse
top level: BBa_K180020
start: 1345
end: 1473
orientation: inline

BBa_R0079
type: SequenceAnnotation
persistent identity: annotation2256456
display id: annotation2256456
version: 1
top level: BBa_K180020
location: https://synbiohub.org/public/igem/BBa_K180020/annotation2256456/range2256456/1
component: BBa_R0079

https://synbiohub.org/public/igem/BBa_K180020/annotation2256456/range2256456/1
type: Range
persistent identity: range2256456
display id: range2256456
version: 1
direction: direction/reverse
top level: BBa_K180020
start: 1
end: 157
orientation: inline

BBa_C0012
type: SequenceAnnotation
persistent identity: annotation2256460
display id: annotation2256460
version: 1
top level: BBa_K180020
location: https://synbiohub.org/public/igem/BBa_K180020/annotation2256460/range2256460/1
component: BBa_C0012

https://synbiohub.org/public/igem/BBa_K180020/annotation2256460/range2256460/1
type: Range
persistent identity: range2256460
display id: range2256460
version: 1
direction: direction/forward
top level: BBa_K180020
start: 184
end: 1311
orientation: inline

Mnt
type: ComponentDefinition
persistent identity: BBa R0073
display id: BBa_R0073
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter (Mnt regulated)
creator: crackdots
created: 2004-01-27T12:00:00Z
modified: 2015-05-08T01:14:15Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable notes: unsure of where -35 site is
mutable provenance: enterobacteriophage p22
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: dimer right half
sequence annotation: dimer left half
sequence annotation: -10
sequence annotation: mnt
sequence: https://synbiohub.org/public/igem/BBa_R0073_sequence/1

dimer right half
type: SequenceAnnotation
persistent identity: annotation318638
display id: annotation318638
version: 1
top level: Mnt
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0073/annotation318638/range318638/1

https://synbiohub.org/public/igem/BBa_R0073/annotation318638/range318638/1
type: Range
persistent identity: range318638
display id: range318638
version: 1
direction: direction/forward
top level: Mnt
start: 32
end: 38
orientation: inline

dimer left half
type: SequenceAnnotation
persistent identity: annotation318636
display id: annotation318636
version: 1
top level: Mnt
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0073/annotation318636/range318636/1

https://synbiohub.org/public/igem/BBa_R0073/annotation318636/range318636/1
type: Range
persistent identity: range318636
display id: range318636
version: 1
direction: direction/forward
top level: Mnt
start: 23
end: 29
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation318643
display id: annotation318643
version: 1
top level: Mnt
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0073/annotation318643/range318643/1

https://synbiohub.org/public/igem/BBa_R0073/annotation318643/range318643/1
type: Range
persistent identity: range318643
display id: range318643
version: 1
direction: direction/forward
top level: Mnt
start: 47
end: 52
orientation: inline

mnt
type: SequenceAnnotation
persistent identity: annotation302669
display id: annotation302669
version: 1
top level: Mnt
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_R0073/annotation302669/range302669/1

https://synbiohub.org/public/igem/BBa_R0073/annotation302669/range302669/1
type: Range
persistent identity: range302669
display id: range302669
version: 1
direction: direction/forward
top level: Mnt
start: 1
end: 67
orientation: inline

BBa_K1520509
type: ComponentDefinition
persistent identity: BBa K1520509
display id: BBa_K1520509
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PgolTS-golS-PgolB-rbs-tetR-Ter-PtetO-rbs-rfp-Ter-Plac-rbs-tetR-Ter-Pcons2-rbs-lacI-Ter
creator: yue wu
created: 2014-09-22T11:00:00Z
modified: 2015-05-08T01:10:49Z
discontinued: false
dominant: false
group u list: _1902_
m user id: 0
owner id: 21318
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: PgolTS-golS-PgolB-rbs-tetR-Ter-PtetO-rbs-rfp-Plac-rbs-tetR-Ter-Pcons2-rbs-lacI-Ter It is a test of our suicide circuit for gold ions.
mutable notes: None
mutable provenance: PgolTS-golS-PgolB, tetR, PtetO, rfp, Plac, tetR, Pcons2, lacI, Ter
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1520026
component: BBa_K1520506
component: BBa_K1520029
component: BBa_K1520505
sequence annotation: BBa_K1520029
sequence annotation: BBa_K1520026
sequence annotation: BBa_K1520506
sequence annotation: BBa_K1520505
sequence: https://synbiohub.org/public/igem/BBa_K1520509_sequence/1

BBa_K1520026
type: Component
persistent identity: component2385559
display id: component2385559
version: 1
top level: BBa_K1520509
access: public
definition: BBa_K1520026

BBa_K1520506
type: Component
persistent identity: component2385527
display id: component2385527
version: 1
top level: BBa_K1520509
access: public
definition: https://synbiohub.org/public/igem/BBa_K1520506/1

BBa_K1520029
type: Component
persistent identity: component2385531
display id: component2385531
version: 1
top level: BBa_K1520509
access: public
definition: https://synbiohub.org/public/igem/BBa_K1520029/1

BBa_K1520505
type: Component
persistent identity: component2385530
display id: component2385530
version: 1
top level: BBa_K1520509
access: public
definition: https://synbiohub.org/public/igem/BBa_K1520505/1

BBa_K1520029
type: SequenceAnnotation
persistent identity: annotation2385531
display id: annotation2385531
version: 1
top level: BBa_K1520509
location: https://synbiohub.org/public/igem/BBa_K1520509/annotation2385531/range2385531/1
component: BBa_K1520029

https://synbiohub.org/public/igem/BBa_K1520509/annotation2385531/range2385531/1
type: Range
persistent identity: range2385531
display id: range2385531
version: 1
direction: direction/reverse
top level: BBa_K1520509
start: 1473
end: 1601
orientation: inline

BBa_K1520026
type: SequenceAnnotation
persistent identity: annotation2385559
display id: annotation2385559
version: 1
top level: BBa_K1520509
location: https://synbiohub.org/public/igem/BBa_K1520509/annotation2385559/range2385559/1
component: BBa_K1520026

https://synbiohub.org/public/igem/BBa_K1520509/annotation2385559/range2385559/1
type: Range
persistent identity: range2385559
display id: range2385559
version: 1
direction: direction/reverse
top level: BBa_K1520509
start: 1610
end: 4955
orientation: inline

BBa_K1520506
type: SequenceAnnotation
persistent identity: annotation2385527
display id: annotation2385527
version: 1
top level: BBa_K1520509
location: https://synbiohub.org/public/igem/BBa_K1520509/annotation2385527/range2385527/1
component: BBa_K1520506

https://synbiohub.org/public/igem/BBa_K1520509/annotation2385527/range2385527/1
type: Range
persistent identity: range2385527
display id: range2385527
version: 1
direction: direction/reverse
top level: BBa_K1520509
start: 1
end: 753
orientation: inline

BBa_K1520505
type: SequenceAnnotation
persistent identity: annotation2385530
display id: annotation2385530
version: 1
top level: BBa_K1520509
location: https://synbiohub.org/public/igem/BBa_K1520509/annotation2385530/range2385530/1
component: BBa_K1520505

https://synbiohub.org/public/igem/BBa_K1520509/annotation2385530/range2385530/1
type: Range
persistent identity: range2385530
display id: range2385530
version: 1
direction: direction/reverse
top level: BBa_K1520509
start: 762
end: 1464
orientation: inline

BBa_I741021
type: ComponentDefinition
persistent identity: BBa I741021
display id: BBa_I741021
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: promoter to xylA without CRP and several binding sites for xylR
creator: Lucien Weiss
created: 2007-06-20T11:00:00Z
modified: 2015-08-31T04:08:02Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _143_
m user id: 0
owner id: 1763
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: removed crp binding site removed rbs removed several xylR binding sites
mutable provenance: ecoli K12 genome
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: promoter
sequence: https://synbiohub.org/public/igem/BBa_I741021_sequence/1

promoter
type: SequenceAnnotation
persistent identity: annotation1935206
display id: annotation1935206
version: 1
top level: BBa_I741021
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I741021/annotation1935206/range1935206/1

https://synbiohub.org/public/igem/BBa_I741021/annotation1935206/range1935206/1
type: Range
persistent identity: range1935206
display id: range1935206
version: 1
direction: direction/reverse
top level: BBa_I741021
start: 15
end: 75
orientation: inline

BBa_J23114
type: ComponentDefinition
persistent identity: BBa J23114
display id: BBa_J23114
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: constitutive promoter family member
creator: John Anderson
created: 2006-08-16T11:00:00Z
modified: 2015-08-31T04:08:40Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: Later
mutable notes: N/A
mutable provenance: Later
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J23114_sequence/1

U073O11+D0
type: ComponentDefinition
persistent identity: BBa I732428
display id: BBa_I732428
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member (U073O11+D038O22)
creator: Zhan Jian
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732428_sequence/1

BBa_K266008
type: ComponentDefinition
persistent identity: BBa K266008
display id: BBa_K266008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: J23100 + Lac inverter
creator: UNAM FCIENCIAS-CINVESTAV IRA
created: 2009-10-12T11:00:00Z
modified: 2015-05-08T01:11:43Z
discontinued: false
dominant: false
experience: experience/None
group u list: _363_
m user id: 0
owner id: 1955
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: The BBa_J23100 constitutive promoter with Lac systems inverter
mutable notes: None
mutable provenance: The biobricks BBa_J23100 and BBa_Q04121 where used to build this biobrick
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Inverter
component: BBa_J23100
component: BBa_Q04121
sequence annotation: BBa_J23100
sequence annotation: BBa_Q04121
sequence: https://synbiohub.org/public/igem/BBa_K266008_sequence/1

BBa_J23100
type: Component
persistent identity: component2262945
display id: component2262945
version: 1
top level: BBa_K266008
access: public
definition: BBa_J23100

BBa_Q04121
type: Component
persistent identity: component2262964
display id: component2262964
version: 1
top level: BBa_K266008
access: public
definition: https://synbiohub.org/public/igem/BBa_Q04121/1

BBa_J23100
type: SequenceAnnotation
persistent identity: annotation2262945
display id: annotation2262945
version: 1
top level: BBa_K266008
location: https://synbiohub.org/public/igem/BBa_K266008/annotation2262945/range2262945/1
component: BBa_J23100

https://synbiohub.org/public/igem/BBa_K266008/annotation2262945/range2262945/1
type: Range
persistent identity: range2262945
display id: range2262945
version: 1
direction: direction/reverse
top level: BBa_K266008
start: 1
end: 35
orientation: inline

BBa_Q04121
type: SequenceAnnotation
persistent identity: annotation2262964
display id: annotation2262964
version: 1
top level: BBa_K266008
location: https://synbiohub.org/public/igem/BBa_K266008/annotation2262964/range2262964/1
component: BBa_Q04121

https://synbiohub.org/public/igem/BBa_K266008/annotation2262964/range2262964/1
type: Range
persistent identity: range2262964
display id: range2262964
version: 1
direction: direction/reverse
top level: BBa_K266008
start: 44
end: 1414
orientation: inline

BBa_J54120
type: ComponentDefinition
persistent identity: BBa J54120
display id: BBa_J54120
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: EmrR_regulated promoter
creator: Yusaku Nakashima
created: 2006-10-20T11:00:00Z
modified: 2015-08-31T03:48:12Z
discontinued: false
dominant: false
group u list: _76_
m user id: 0
owner id: 762
owning group id: 76
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: EmrR_regulated promoter
mutable notes: AS EmrR regulated
mutable provenance: DNA synthesis
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BamHI
sequence annotation: BetI_regulated promoter
sequence: https://synbiohub.org/public/igem/BBa_J54120_sequence/1

BamHI
type: SequenceAnnotation
persistent identity: annotation1903850
display id: annotation1903850
version: 1
top level: BBa_J54120
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_J54120/annotation1903850/range1903850/1

https://synbiohub.org/public/igem/BBa_J54120/annotation1903850/range1903850/1
type: Range
persistent identity: range1903850
display id: range1903850
version: 1
direction: direction/forward
top level: BBa_J54120
start: 1
end: 5
orientation: inline

BetI_regulated promoter
type: SequenceAnnotation
persistent identity: annotation1903851
display id: annotation1903851
version: 1
top level: BBa_J54120
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_J54120/annotation1903851/range1903851/1

https://synbiohub.org/public/igem/BBa_J54120/annotation1903851/range1903851/1
type: Range
persistent identity: range1903851
display id: range1903851
version: 1
direction: direction/forward
top level: BBa_J54120
start: 6
end: 43
orientation: inline

BBa_I739103
type: ComponentDefinition
persistent identity: BBa I739103
display id: BBa_I739103
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Double Promoter (lacI, negative / P22 cII, negative)
creator: Stefan Luzi
created: 2007-10-14T11:00:00Z
modified: 2015-08-31T04:08:01Z
discontinued: false
dominant: false
group u list: _124_
m user id: 0
owner id: 2183
owning group id: 62
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: asdf
mutable notes: asdf
mutable provenance: asf
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: OR1
sequence annotation: R0011
sequence annotation: R0053 (2x OR1)
sequence: https://synbiohub.org/public/igem/BBa_I739103_sequence/1

OR1
type: SequenceAnnotation
persistent identity: annotation1947821
display id: annotation1947821
version: 1
top level: BBa_I739103
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I739103/annotation1947821/range1947821/1

https://synbiohub.org/public/igem/BBa_I739103/annotation1947821/range1947821/1
type: Range
persistent identity: range1947821
display id: range1947821
version: 1
direction: direction/forward
top level: BBa_I739103
start: 50
end: 68
orientation: inline

R0011
type: SequenceAnnotation
persistent identity: annotation1947820
display id: annotation1947820
version: 1
top level: BBa_I739103
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I739103/annotation1947820/range1947820/1

https://synbiohub.org/public/igem/BBa_I739103/annotation1947820/range1947820/1
type: Range
persistent identity: range1947820
display id: range1947820
version: 1
direction: direction/forward
top level: BBa_I739103
start: 1
end: 48
orientation: inline

R0053 (2x OR1)
type: SequenceAnnotation
persistent identity: annotation1947823
display id: annotation1947823
version: 1
top level: BBa_I739103
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_I739103/annotation1947823/range1947823/1

https://synbiohub.org/public/igem/BBa_I739103/annotation1947823/range1947823/1
type: Range
persistent identity: range1947823
display id: range1947823
version: 1
direction: direction/forward
top level: BBa_I739103
start: 50
end: 87
orientation: inline

OR1
type: SequenceAnnotation
persistent identity: annotation1947822
display id: annotation1947822
version: 1
top level: BBa_I739103
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I739103/annotation1947822/range1947822/1

https://synbiohub.org/public/igem/BBa_I739103/annotation1947822/range1947822/1
type: Range
persistent identity: range1947822
display id: range1947822
version: 1
direction: direction/forward
top level: BBa_I739103
start: 69
end: 87
orientation: inline

BBa_J64712
type: ComponentDefinition
persistent identity: BBa J64712
display id: BBa_J64712
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LasR/LasI Inducible & RHLR/RHLI repressible Promoter
creator: laura lavery
created: 2007-03-24T12:00:00Z
modified: 2015-05-08T01:08:17Z
discontinued: false
dominant: false
experience: experience/None
group u list: _98_
m user id: 0
owner id: 1431
owning group id: 98
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This part is similar to BBa_R0079 but has an added binding site for RHLR/RHLI in between the -35 and -10 region. Thus this promoter is induced by LasR/LasI but repressed by RHLR/RHLI binding.
mutable notes: The natural spacer between the -35 and -10 is 14bp and the natural O1 site for RHLR/RHLI is 20bp long. There was not much over lap of the -35 or the -10 with this sequence, so it was nessicary to chop off some of the binding site sequence in order to maintain the correct spacing and preserve the -35 and -10 sequence. The binding site sequence is missing a tcc on is 5' end and a t on its 3' end.
mutable provenance: Rust L. et. al., "Analysis of the Pseudomonas aeruginosa Elastase (lasB) Regulatory Region". Journal of Bacteriology, 1996.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: LasR/LasI O2
sequence annotation: -35
sequence annotation: -10
sequence annotation: LasR/LasI O1
sequence annotation: RHLR/RHLI
sequence: https://synbiohub.org/public/igem/BBa_J64712_sequence/1

LasR/LasI O2
type: SequenceAnnotation
persistent identity: annotation1921064
display id: annotation1921064
version: 1
top level: BBa_J64712
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_J64712/annotation1921064/range1921064/1

https://synbiohub.org/public/igem/BBa_J64712/annotation1921064/range1921064/1
type: Range
persistent identity: range1921064
display id: range1921064
version: 1
direction: direction/forward
top level: BBa_J64712
start: 46
end: 64
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1921067
display id: annotation1921067
version: 1
top level: BBa_J64712
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J64712/annotation1921067/range1921067/1

https://synbiohub.org/public/igem/BBa_J64712/annotation1921067/range1921067/1
type: Range
persistent identity: range1921067
display id: range1921067
version: 1
direction: direction/forward
top level: BBa_J64712
start: 117
end: 122
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1921068
display id: annotation1921068
version: 1
top level: BBa_J64712
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J64712/annotation1921068/range1921068/1

https://synbiohub.org/public/igem/BBa_J64712/annotation1921068/range1921068/1
type: Range
persistent identity: range1921068
display id: range1921068
version: 1
direction: direction/forward
top level: BBa_J64712
start: 140
end: 145
orientation: inline

LasR/LasI O1
type: SequenceAnnotation
persistent identity: annotation1921065
display id: annotation1921065
version: 1
top level: BBa_J64712
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_J64712/annotation1921065/range1921065/1

https://synbiohub.org/public/igem/BBa_J64712/annotation1921065/range1921065/1
type: Range
persistent identity: range1921065
display id: range1921065
version: 1
direction: direction/forward
top level: BBa_J64712
start: 106
end: 125
orientation: inline

RHLR/RHLI
type: SequenceAnnotation
persistent identity: annotation1921066
display id: annotation1921066
version: 1
top level: BBa_J64712
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_J64712/annotation1921066/range1921066/1

https://synbiohub.org/public/igem/BBa_J64712/annotation1921066/range1921066/1
type: Range
persistent identity: range1921066
display id: range1921066
version: 1
direction: direction/forward
top level: BBa_J64712
start: 125
end: 141
orientation: inline

BBa_K1585104
type: ComponentDefinition
persistent identity: BBa K1585104
display id: BBa_K1585104
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Anderson Promoter with lacI binding site
creator: Tobias Schwanemann
created: 2015-08-31T11:00:00Z
modified: 2015-09-01T03:26:03Z
discontinued: false
dominant: false
experience: experience/Fails
group u list: _2002_
m user id: 24474
owner id: 24474
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Promoters of the Anderson Promoter Collection with lacI binding site derived from [http://parts.igem.org/Part:BBa_R0010 R_0010] to make them [http://openwetware.org/wiki/IPTG IPTG] iducible.
mutable notes: We choosed lacI-binding-site of part BBa_R0010 because we assumed older parts would be more reliable. The part is shipped in backbone [http://parts.igem.org/Part:BBa_J61002 J61002] because the functionality is directly visible by adding IPTG. RFP ([http://parts.igem.org/Part:BBa_E1010 E1010]) should get expressed then.
mutable provenance: Assambly of [http://parts.igem.org/Part:BBa_J23104 J23104] and lacI-binding-site of [http://parts.igem.org/Part:BBa_R0010 R_0010] The "lacI binding site" of Part [http://parts.igem.org/Part:BBa_R0010 R_0010] differs from lacO from Part [http://parts.igem.org/Part:BBa_J33207 BBa_J33207].
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
component: BBa_K1585999
component: BBa_J23104
sequence annotation: BBa_J23104
sequence annotation: BBa_K1585999
sequence: https://synbiohub.org/public/igem/BBa_K1585104_sequence/1

BBa_K1585999
type: Component
persistent identity: component2441422
display id: component2441422
version: 1
top level: BBa_K1585104
access: public
definition: https://synbiohub.org/public/igem/BBa_K1585999/1

BBa_J23104
type: Component
persistent identity: component2441420
display id: component2441420
version: 1
top level: BBa_K1585104
access: public
definition: BBa_J23104

BBa_J23104
type: SequenceAnnotation
persistent identity: annotation2441420
display id: annotation2441420
version: 1
top level: BBa_K1585104
location: https://synbiohub.org/public/igem/BBa_K1585104/annotation2441420/range2441420/1
component: BBa_J23104

https://synbiohub.org/public/igem/BBa_K1585104/annotation2441420/range2441420/1
type: Range
persistent identity: range2441420
display id: range2441420
version: 1
direction: direction/reverse
top level: BBa_K1585104
start: 1
end: 35
orientation: inline

BBa_K1585999
type: SequenceAnnotation
persistent identity: annotation2441422
display id: annotation2441422
version: 1
top level: BBa_K1585104
location: https://synbiohub.org/public/igem/BBa_K1585104/annotation2441422/range2441422/1
component: BBa_K1585999

https://synbiohub.org/public/igem/BBa_K1585104/annotation2441422/range2441422/1
type: Range
persistent identity: range2441422
display id: range2441422
version: 1
direction: direction/reverse
top level: BBa_K1585104
start: 44
end: 78
orientation: inline

BBa_J23150
type: ComponentDefinition
persistent identity: BBa J23150
display id: BBa_J23150
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: 1bp mutant from J23107
creator: Jason Kelly
created: 2008-02-11T12:00:00Z
modified: 2015-08-31T04:08:41Z
discontinued: false
dominant: true
group u list: _11_
m user id: 0
owner id: 2
owning group id: 84
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: .
mutable notes: .
mutable provenance: ..
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J23150_sequence/1

BBa_K180002
type: ComponentDefinition
persistent identity: BBa K180002
display id: BBa_K180002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: tac pR testing plasmid (GFP)
creator: Richard Wilson, Stephanie Chambers
created: 2009-10-13T11:00:00Z
modified: 2015-05-08T01:11:06Z
discontinued: false
dominant: false
experience: experience/None
group u list: _278_
m user id: 0
owner id: 4082
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: PTAC promoter followed by an RBS, green fluorescent protein and stop codon, for testing the effectiveness of the PTAC hybrid promoter designed by iGEM2009 Team:Southampton.
mutable notes: Inclusion of the RBS and stop sequence to ensure correct functionality observed.
mutable provenance: K180000 and existing biobrick parts.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0010
component: BBa_E0040
component: BBa_K180000
component: BBa_B0034
component: BBa_B0012
sequence annotation: BBa_K180000
sequence annotation: BBa_E0040
sequence annotation: BBa_B0012
sequence annotation: BBa_B0034
sequence annotation: BBa_B0010
sequence: https://synbiohub.org/public/igem/BBa_K180002_sequence/1

BBa_B0010
type: Component
persistent identity: component2037772
display id: component2037772
version: 1
top level: BBa_K180002
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_E0040
type: Component
persistent identity: component2037771
display id: component2037771
version: 1
top level: BBa_K180002
access: public
definition: https://synbiohub.org/public/igem/BBa_E0040/1

BBa_K180000
type: Component
persistent identity: component2037767
display id: component2037767
version: 1
top level: BBa_K180002
access: public
definition: BBa_K180000

BBa_B0034
type: Component
persistent identity: component2037769
display id: component2037769
version: 1
top level: BBa_K180002
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0012
type: Component
persistent identity: component2037774
display id: component2037774
version: 1
top level: BBa_K180002
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_K180000
type: SequenceAnnotation
persistent identity: annotation2037767
display id: annotation2037767
version: 1
top level: BBa_K180002
location: https://synbiohub.org/public/igem/BBa_K180002/annotation2037767/range2037767/1
component: BBa_K180000

https://synbiohub.org/public/igem/BBa_K180002/annotation2037767/range2037767/1
type: Range
persistent identity: range2037767
display id: range2037767
version: 1
direction: direction/reverse
top level: BBa_K180002
start: 1
end: 1447
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation2037771
display id: annotation2037771
version: 1
top level: BBa_K180002
location: https://synbiohub.org/public/igem/BBa_K180002/annotation2037771/range2037771/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_K180002/annotation2037771/range2037771/1
type: Range
persistent identity: range2037771
display id: range2037771
version: 1
direction: direction/reverse
top level: BBa_K180002
start: 1474
end: 2193
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2037774
display id: annotation2037774
version: 1
top level: BBa_K180002
location: https://synbiohub.org/public/igem/BBa_K180002/annotation2037774/range2037774/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K180002/annotation2037774/range2037774/1
type: Range
persistent identity: range2037774
display id: range2037774
version: 1
direction: direction/forward
top level: BBa_K180002
start: 2290
end: 2330
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2037769
display id: annotation2037769
version: 1
top level: BBa_K180002
location: https://synbiohub.org/public/igem/BBa_K180002/annotation2037769/range2037769/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K180002/annotation2037769/range2037769/1
type: Range
persistent identity: range2037769
display id: range2037769
version: 1
direction: direction/reverse
top level: BBa_K180002
start: 1456
end: 1467
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2037772
display id: annotation2037772
version: 1
top level: BBa_K180002
location: https://synbiohub.org/public/igem/BBa_K180002/annotation2037772/range2037772/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K180002/annotation2037772/range2037772/1
type: Range
persistent identity: range2037772
display id: range2037772
version: 1
direction: direction/forward
top level: BBa_K180002
start: 2202
end: 2281
orientation: inline

BBa_K1585119
type: ComponentDefinition
persistent identity: BBa K1585119
display id: BBa_K1585119
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Anderson Promoter with lacI binding site
creator: Tobias Schwanemann
created: 2015-08-31T11:00:00Z
modified: 2015-09-01T02:33:36Z
discontinued: false
dominant: false
experience: experience/Fails
group u list: _2002_
m user id: 24474
owner id: 24474
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Promoters of the Anderson Promoter Collection with lacI binding site derived from [http://parts.igem.org/Part:BBa_R0010 R_0010] to make them [http://openwetware.org/wiki/IPTG IPTG] iducible.
mutable notes: We choosed lacI-binding-site of part BBa_R0010 because we assumed older parts would be more reliable. The part is shipped in backbone [http://parts.igem.org/Part:BBa_J61002 J61002] because the functionality is directly visible by adding IPTG. RFP ([http://parts.igem.org/Part:BBa_E1010 E1010]) should get expressed then.
mutable provenance: Assambly of [http://parts.igem.org/Part:BBa_J23119 J23119] and lacI-binding-site of [http://parts.igem.org/Part:BBa_R0010 R_0010] The "lacI binding site" of Part [http://parts.igem.org/Part:BBa_R0010 R_0010] differs from lacO from Part [http://parts.igem.org/Part:BBa_J33207 BBa_J33207].
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
component: BBa_J23119
component: BBa_K1585999
sequence annotation: BBa_J23119
sequence annotation: BBa_K1585999
sequence: https://synbiohub.org/public/igem/BBa_K1585119_sequence/1

BBa_J23119
type: Component
persistent identity: component2441719
display id: component2441719
version: 1
top level: BBa_K1585119
access: public
definition: BBa_J23119

BBa_K1585999
type: Component
persistent identity: component2441721
display id: component2441721
version: 1
top level: BBa_K1585119
access: public
definition: https://synbiohub.org/public/igem/BBa_K1585999/1

BBa_J23119
type: SequenceAnnotation
persistent identity: annotation2441719
display id: annotation2441719
version: 1
top level: BBa_K1585119
location: https://synbiohub.org/public/igem/BBa_K1585119/annotation2441719/range2441719/1
component: BBa_J23119

https://synbiohub.org/public/igem/BBa_K1585119/annotation2441719/range2441719/1
type: Range
persistent identity: range2441719
display id: range2441719
version: 1
direction: direction/reverse
top level: BBa_K1585119
start: 1
end: 35
orientation: inline

BBa_K1585999
type: SequenceAnnotation
persistent identity: annotation2441721
display id: annotation2441721
version: 1
top level: BBa_K1585119
location: https://synbiohub.org/public/igem/BBa_K1585119/annotation2441721/range2441721/1
component: BBa_K1585999

https://synbiohub.org/public/igem/BBa_K1585119/annotation2441721/range2441721/1
type: Range
persistent identity: range2441721
display id: range2441721
version: 1
direction: direction/reverse
top level: BBa_K1585119
start: 44
end: 78
orientation: inline

BBa_J72005
type: ComponentDefinition
persistent identity: BBa J72005
display id: BBa_J72005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: {Ptet} promoter in BBb
creator: John Anderson
created: 2008-07-15T11:00:00Z
modified: 2015-05-08T01:08:26Z
discontinued: false
dominant: false
group u list: _171_
m user id: 0
owner id: 483
owning group id: 171
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Equivalent part to r0040, but in BBb format. In house, part is referred to as pBca9145-Bca1091 {Ptet}. ---- This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at: [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
mutable notes: N/A
mutable provenance: PCR ca1091F/R on pSB1A2-r0040 (~100 bp, EcoRI/BamHI) Sub into pBca9145-Bca1089 (EcoRI/BamHI) Product is pBca9145-Bca1091 ---- ca1091F Forward EcoRI/BglII for Ptet part ttctggaattcatgAGATCTtccctatcagtgatagag ca1091R Reverse BamHI for Ptet part GtttGGATCCgtgctcagtatctctatc
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J72005_sequence/1

cII p22
type: ComponentDefinition
persistent identity: BBa R1053
display id: BBa_R1053
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter, Standard (p22 cII regulated)
creator: Maia Mahoney, Drew Endy
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:15Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: [Note: This is the same part as R0053 except that the -10 and -35 sites and spacing have been changed to comply with BBa_S0001]. The p22 cII regulatory region sequence is a 97 base-pair sequence with the standard BioBrick prefix and suffix sections on its ends. p22 cII repressor protein, BBa_C0053, binds to it. This segment contains O-R1, O-R2, a fragment of O-R3, the -35 of P-RM, and P-R (-10 and -35 from Tom Knight)
mutable notes: Modified from wild-type to comply with BBa_S0001: TTGACA-17N-GATACT
mutable provenance: Bacteriophage p22.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: OR1
sequence annotation: -10
sequence annotation: BBa_R1053
sequence annotation: OR2
sequence annotation: OR3
sequence annotation: -35
sequence: https://synbiohub.org/public/igem/BBa_R1053_sequence/1

OR1
type: SequenceAnnotation
persistent identity: annotation2090
display id: annotation2090
version: 1
top level: cII p22
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R1053/annotation2090/range2090/1

https://synbiohub.org/public/igem/BBa_R1053/annotation2090/range2090/1
type: Range
persistent identity: range2090
display id: range2090
version: 1
direction: direction/forward
top level: cII p22
start: 35
end: 52
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2095
display id: annotation2095
version: 1
top level: cII p22
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R1053/annotation2095/range2095/1

https://synbiohub.org/public/igem/BBa_R1053/annotation2095/range2095/1
type: Range
persistent identity: range2095
display id: range2095
version: 1
direction: direction/forward
top level: cII p22
start: 31
end: 36
orientation: inline

BBa_R1053
type: SequenceAnnotation
persistent identity: annotation7076
display id: annotation7076
version: 1
top level: cII p22
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_R1053/annotation7076/range7076/1

https://synbiohub.org/public/igem/BBa_R1053/annotation7076/range7076/1
type: Range
persistent identity: range7076
display id: range7076
version: 1
direction: direction/forward
top level: cII p22
start: 1
end: 55
orientation: inline

OR2
type: SequenceAnnotation
persistent identity: annotation2089
display id: annotation2089
version: 1
top level: cII p22
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R1053/annotation2089/range2089/1

https://synbiohub.org/public/igem/BBa_R1053/annotation2089/range2089/1
type: Range
persistent identity: range2089
display id: range2089
version: 1
direction: direction/forward
top level: cII p22
start: 11
end: 29
orientation: inline

OR3
type: SequenceAnnotation
persistent identity: annotation2088
display id: annotation2088
version: 1
top level: cII p22
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R1053/annotation2088/range2088/1

https://synbiohub.org/public/igem/BBa_R1053/annotation2088/range2088/1
type: Range
persistent identity: range2088
display id: range2088
version: 1
direction: direction/forward
top level: cII p22
start: 1
end: 3
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2091
display id: annotation2091
version: 1
top level: cII p22
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R1053/annotation2091/range2091/1

https://synbiohub.org/public/igem/BBa_R1053/annotation2091/range2091/1
type: Range
persistent identity: range2091
display id: range2091
version: 1
direction: direction/forward
top level: cII p22
start: 19
end: 24
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2094
display id: annotation2094
version: 1
top level: cII p22
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R1053/annotation2094/range2094/1

https://synbiohub.org/public/igem/BBa_R1053/annotation2094/range2094/1
type: Range
persistent identity: range2094
display id: range2094
version: 1
direction: direction/forward
top level: cII p22
start: 8
end: 14
orientation: inline

Plac(rev)
type: ComponentDefinition
persistent identity: BBa I742124
display id: BBa_I742124
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Reverse complement Lac promoter
creator: Caroline Dahl
created: 2007-10-21T11:00:00Z
modified: 2015-08-31T04:08:02Z
discontinued: false
dominant: false
experience: experience/None
group u list: _123_
m user id: 0
owner id: 1968
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This is an 'upside-down' version of I14032. It is a strong, constitual promoter. By adding this part, we wish to emphasise the possibility of engineering both DNA strands, and the additional reglatory possibility that this gives. The upstream sequence is non-functional and simply there to faciliate gel analysis or to physically enable the part to be recomineered (see the Edinburgh 2007 project where it is flanked by dif-sites).
mutable notes: When dealing with reverse complements of a locus, suffix and prefix need be fitted in opposite order.
mutable provenance: This sequence is part of the E.coli K12 genome.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: Plac -35
sequence annotation: CAP binding site
sequence annotation: Plac -10
sequence annotation: LacI binding site
sequence: https://synbiohub.org/public/igem/BBa_I742124_sequence/1

Plac -35
type: SequenceAnnotation
persistent identity: annotation1955298
display id: annotation1955298
version: 1
top level: Plac(rev)
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I742124/annotation1955298/range1955298/1

https://synbiohub.org/public/igem/BBa_I742124/annotation1955298/range1955298/1
type: Range
persistent identity: range1955298
display id: range1955298
version: 1
direction: direction/reverse
top level: Plac(rev)
start: 61
end: 66
orientation: inline

CAP binding site
type: SequenceAnnotation
persistent identity: annotation1955266
display id: annotation1955266
version: 1
top level: Plac(rev)
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_I742124/annotation1955266/range1955266/1

https://synbiohub.org/public/igem/BBa_I742124/annotation1955266/range1955266/1
type: Range
persistent identity: range1955266
display id: range1955266
version: 1
direction: direction/reverse
top level: Plac(rev)
start: 78
end: 115
orientation: inline

Plac -10
type: SequenceAnnotation
persistent identity: annotation1955299
display id: annotation1955299
version: 1
top level: Plac(rev)
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I742124/annotation1955299/range1955299/1

https://synbiohub.org/public/igem/BBa_I742124/annotation1955299/range1955299/1
type: Range
persistent identity: range1955299
display id: range1955299
version: 1
direction: direction/reverse
top level: Plac(rev)
start: 38
end: 43
orientation: inline

LacI binding site
type: SequenceAnnotation
persistent identity: annotation1955267
display id: annotation1955267
version: 1
top level: Plac(rev)
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_I742124/annotation1955267/range1955267/1

https://synbiohub.org/public/igem/BBa_I742124/annotation1955267/range1955267/1
type: Range
persistent identity: range1955267
display id: range1955267
version: 1
direction: direction/reverse
top level: Plac(rev)
start: 11
end: 31
orientation: inline

U097NUL+D0
type: ComponentDefinition
persistent identity: BBa I732400
display id: BBa_I732400
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member (U097NUL+D062NUL)
creator: Zhan Jian
created: 2007-10-03T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1572
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: P_template1 serves as the truss for PCR cloning. By respectively applying 7 different primers upstream and downstream, that is, 49 pairs of primers in total, we have got 49 different promoters. Then we made them digsted and loaded into a vector that has already possessed a lacZ-alpha fragment. In this way, we can read from the color of the clones on the plate whether the inserted promoter works or not. By this means, We can try seven different positions for Operator A and seven for Operator B, thus constructing 7*7 kinds of promoters which have actually constituted a specific promoter family. After transformed into the four test plasmids (pCR00, pCR01, pCR10, pCR11) together with a downstream double-reporter system, we will get to know the solo-repression and co-repression effects of the two repressors on the specific promoter.
mutable notes: None.
mutable provenance: None.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732400_sequence/1

lux pR
type: ComponentDefinition
persistent identity: BBa R0062
display id: BBa_R0062
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter (luxR & HSL regulated -- lux pR)
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:15Z
discontinued: false
dominant: true
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Promoter activated by LuxR in concert with HSL The lux cassette of V. fischeri contains a left and a right promoter. The right promoter gives weak constitutive expression of downstream genes.This expression is up-regulated by the action of the LuxR activator protein complexed with the autoinducer, 3-oxo-hexanoyl-HSL. Two molecules of LuxR protein form a complex with two molecules of the signalling compound homoserine lactone (HSL). This complex binds to a palindromic site on the promoter, increasing the rate of transcription.
mutable notes: This promoter is based on the Vibrio fischeri quorum sensing gene promoters. Two genes LuxI and LuxR and transcribed in opposite directions as shown below. The original sequence from which the parts <bb_part>BBa_R0062</bb_part> and <bb_part>BBa_R0063</bb_part> were derived is shown in the picture below. <img src="<bb_file>Image1.gif</bb_file>" width="614" height="362">
mutable provenance: V. fischeri
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -35
sequence annotation: LuxR/HSL
sequence annotation: -10
sequence annotation: start
sequence annotation: BBa_R0062
sequence: https://synbiohub.org/public/igem/BBa_R0062_sequence/1

-35
type: SequenceAnnotation
persistent identity: annotation2046
display id: annotation2046
version: 1
top level: lux pR
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0062/annotation2046/range2046/1

https://synbiohub.org/public/igem/BBa_R0062/annotation2046/range2046/1
type: Range
persistent identity: range2046
display id: range2046
version: 1
direction: direction/forward
top level: lux pR
start: 20
end: 25
orientation: inline

LuxR/HSL
type: SequenceAnnotation
persistent identity: annotation2045
display id: annotation2045
version: 1
top level: lux pR
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0062/annotation2045/range2045/1

https://synbiohub.org/public/igem/BBa_R0062/annotation2045/range2045/1
type: Range
persistent identity: range2045
display id: range2045
version: 1
direction: direction/forward
top level: lux pR
start: 1
end: 20
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2047
display id: annotation2047
version: 1
top level: lux pR
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0062/annotation2047/range2047/1

https://synbiohub.org/public/igem/BBa_R0062/annotation2047/range2047/1
type: Range
persistent identity: range2047
display id: range2047
version: 1
direction: direction/forward
top level: lux pR
start: 42
end: 47
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2048
display id: annotation2048
version: 1
top level: lux pR
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_R0062/annotation2048/range2048/1

https://synbiohub.org/public/igem/BBa_R0062/annotation2048/range2048/1
type: Range
persistent identity: range2048
display id: range2048
version: 1
direction: direction/forward
top level: lux pR
start: 53
end: 53
orientation: inline

BBa_R0062
type: SequenceAnnotation
persistent identity: annotation7070
display id: annotation7070
version: 1
top level: lux pR
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_R0062/annotation7070/range7070/1

https://synbiohub.org/public/igem/BBa_R0062/annotation7070/range7070/1
type: Range
persistent identity: range7070
display id: range7070
version: 1
direction: direction/forward
top level: lux pR
start: 1
end: 55
orientation: inline

U085O11+D0
type: ComponentDefinition
persistent identity: BBa I732419
display id: BBa_I732419
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member (U085O11+D002O22)
creator: Zhan Jian
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732419_sequence/1

Pnot(O11)
type: ComponentDefinition
persistent identity: BBa I732201
display id: BBa_I732201
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: NOT Gate Promoter Family Member (D001O11)
creator: Zhan Jian
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:57Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732201_sequence/1

BBa_K266005
type: ComponentDefinition
persistent identity: BBa K266005
display id: BBa_K266005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PAI+LasR -> LasI & AI+LuxR --| LasI
creator: UNAM FCIENCIAS-CINVESTAV IRA
created: 2009-10-12T11:00:00Z
modified: 2015-05-08T01:11:43Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _363_
m user id: 0
owner id: 1955
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: BBa_K091146 promoter (PAI+LasR inducible & AI+LuxR repressible) controls the expresion of LasI enzyme, no terminator
mutable notes: None
mutable provenance: The biobricks BBa_K091146 and BBa_S03154 where used to build this biobrick
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Signalling
role: SO:0000804
component: BBa_S03154
component: BBa_K091146
sequence annotation: BBa_K091146
sequence annotation: BBa_S03154
sequence: https://synbiohub.org/public/igem/BBa_K266005_sequence/1

BBa_S03154
type: Component
persistent identity: component2246684
display id: component2246684
version: 1
top level: BBa_K266005
access: public
definition: https://synbiohub.org/public/igem/BBa_S03154/1

BBa_K091146
type: Component
persistent identity: component2246677
display id: component2246677
version: 1
top level: BBa_K266005
access: public
definition: BBa_K091146

BBa_K091146
type: SequenceAnnotation
persistent identity: annotation2246677
display id: annotation2246677
version: 1
top level: BBa_K266005
location: https://synbiohub.org/public/igem/BBa_K266005/annotation2246677/range2246677/1
component: BBa_K091146

https://synbiohub.org/public/igem/BBa_K266005/annotation2246677/range2246677/1
type: Range
persistent identity: range2246677
display id: range2246677
version: 1
direction: direction/reverse
top level: BBa_K266005
start: 1
end: 126
orientation: inline

BBa_S03154
type: SequenceAnnotation
persistent identity: annotation2246684
display id: annotation2246684
version: 1
top level: BBa_K266005
location: https://synbiohub.org/public/igem/BBa_K266005/annotation2246684/range2246684/1
component: BBa_S03154

https://synbiohub.org/public/igem/BBa_K266005/annotation2246684/range2246684/1
type: Range
persistent identity: range2246684
display id: range2246684
version: 1
direction: direction/reverse
top level: BBa_K266005
start: 135
end: 819
orientation: inline

U037O11+D0
type: ComponentDefinition
persistent identity: BBa I732406
display id: BBa_I732406
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member (U037O11+D062NUL)
creator: Zhan Jian
created: 2007-09-19T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1572
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: A Double Reporter (LacZ-alpha and GFP-AAV) loaded on pSB3K5. For more information please refer to BBa_I732403.
mutable notes: Might be later edited.
mutable provenance: Might be later edited.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BglII
sequence annotation: GFP protein
sequence annotation: STOP
sequence annotation: stem_loop
sequence annotation: stop
sequence annotation: BBa_I732007
sequence annotation: BBa_E0040
sequence annotation: BBa_B0034
sequence annotation: BamHI
sequence annotation: polya
sequence annotation: T7 TE
sequence annotation: conserved
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: START
sequence annotation: BBa_I732006
sequence annotation: lacZ
sequence: https://synbiohub.org/public/igem/BBa_I732406_sequence/1

BglII
type: SequenceAnnotation
persistent identity: annotation1944460
display id: annotation1944460
version: 1
top level: U037O11+D0
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I732406/annotation1944460/range1944460/1

https://synbiohub.org/public/igem/BBa_I732406/annotation1944460/range1944460/1
type: Range
persistent identity: range1944460
display id: range1944460
version: 1
direction: direction/forward
top level: U037O11+D0
start: 3
end: 8
orientation: inline

GFP protein
type: SequenceAnnotation
persistent identity: annotation1944471
display id: annotation1944471
version: 1
top level: U037O11+D0
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_I732406/annotation1944471/range1944471/1

https://synbiohub.org/public/igem/BBa_I732406/annotation1944471/range1944471/1
type: Range
persistent identity: range1944471
display id: range1944471
version: 1
direction: direction/forward
top level: U037O11+D0
start: 306
end: 1025
orientation: inline

STOP
type: SequenceAnnotation
persistent identity: annotation1944467
display id: annotation1944467
version: 1
top level: U037O11+D0
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_I732406/annotation1944467/range1944467/1

https://synbiohub.org/public/igem/BBa_I732406/annotation1944467/range1944467/1
type: Range
persistent identity: range1944467
display id: range1944467
version: 1
direction: direction/forward
top level: U037O11+D0
start: 274
end: 279
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation1944474
display id: annotation1944474
version: 1
top level: U037O11+D0
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_I732406/annotation1944474/range1944474/1

https://synbiohub.org/public/igem/BBa_I732406/annotation1944474/range1944474/1
type: Range
persistent identity: range1944474
display id: range1944474
version: 1
direction: direction/forward
top level: U037O11+D0
start: 1045
end: 1088
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1944478
display id: annotation1944478
version: 1
top level: U037O11+D0
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_I732406/annotation1944478/range1944478/1

https://synbiohub.org/public/igem/BBa_I732406/annotation1944478/range1944478/1
type: Range
persistent identity: range1944478
display id: range1944478
version: 1
direction: direction/forward
top level: U037O11+D0
start: 1155
end: 1155
orientation: inline

BBa_I732007
type: SequenceAnnotation
persistent identity: annotation1944462
display id: annotation1944462
version: 1
top level: U037O11+D0
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I732406/annotation1944462/range1944462/1

https://synbiohub.org/public/igem/BBa_I732406/annotation1944462/range1944462/1
type: Range
persistent identity: range1944462
display id: range1944462
version: 1
direction: direction/reverse
top level: U037O11+D0
start: 1
end: 19
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation1944472
display id: annotation1944472
version: 1
top level: U037O11+D0
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I732406/annotation1944472/range1944472/1

https://synbiohub.org/public/igem/BBa_I732406/annotation1944472/range1944472/1
type: Range
persistent identity: range1944472
display id: range1944472
version: 1
direction: direction/reverse
top level: U037O11+D0
start: 306
end: 1025
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1944470
display id: annotation1944470
version: 1
top level: U037O11+D0
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I732406/annotation1944470/range1944470/1

https://synbiohub.org/public/igem/BBa_I732406/annotation1944470/range1944470/1
type: Range
persistent identity: range1944470
display id: range1944470
version: 1
direction: direction/reverse
top level: U037O11+D0
start: 288
end: 299
orientation: inline

BamHI
type: SequenceAnnotation
persistent identity: annotation1944461
display id: annotation1944461
version: 1
top level: U037O11+D0
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I732406/annotation1944461/range1944461/1

https://synbiohub.org/public/igem/BBa_I732406/annotation1944461/range1944461/1
type: Range
persistent identity: range1944461
display id: range1944461
version: 1
direction: direction/forward
top level: U037O11+D0
start: 12
end: 17
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1944477
display id: annotation1944477
version: 1
top level: U037O11+D0
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_I732406/annotation1944477/range1944477/1

https://synbiohub.org/public/igem/BBa_I732406/annotation1944477/range1944477/1
type: Range
persistent identity: range1944477
display id: range1944477
version: 1
direction: direction/forward
top level: U037O11+D0
start: 1149
end: 1162
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1944476
display id: annotation1944476
version: 1
top level: U037O11+D0
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_I732406/annotation1944476/range1944476/1

https://synbiohub.org/public/igem/BBa_I732406/annotation1944476/range1944476/1
type: Range
persistent identity: range1944476
display id: range1944476
version: 1
direction: direction/forward
top level: U037O11+D0
start: 1129
end: 1148
orientation: inline

conserved
type: SequenceAnnotation
persistent identity: annotation1944469
display id: annotation1944469
version: 1
top level: U037O11+D0
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_I732406/annotation1944469/range1944469/1

https://synbiohub.org/public/igem/BBa_I732406/annotation1944469/range1944469/1
type: Range
persistent identity: range1944469
display id: range1944469
version: 1
direction: direction/forward
top level: U037O11+D0
start: 292
end: 295
orientation: inline

conserved
type: SequenceAnnotation
persistent identity: annotation1944463
display id: annotation1944463
version: 1
top level: U037O11+D0
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_I732406/annotation1944463/range1944463/1

https://synbiohub.org/public/igem/BBa_I732406/annotation1944463/range1944463/1
type: Range
persistent identity: range1944463
display id: range1944463
version: 1
direction: direction/forward
top level: U037O11+D0
start: 32
end: 35
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1944473
display id: annotation1944473
version: 1
top level: U037O11+D0
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I732406/annotation1944473/range1944473/1

https://synbiohub.org/public/igem/BBa_I732406/annotation1944473/range1944473/1
type: Range
persistent identity: range1944473
display id: range1944473
version: 1
direction: direction/forward
top level: U037O11+D0
start: 1034
end: 1113
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1944464
display id: annotation1944464
version: 1
top level: U037O11+D0
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I732406/annotation1944464/range1944464/1

https://synbiohub.org/public/igem/BBa_I732406/annotation1944464/range1944464/1
type: Range
persistent identity: range1944464
display id: range1944464
version: 1
direction: direction/reverse
top level: U037O11+D0
start: 28
end: 39
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1944475
display id: annotation1944475
version: 1
top level: U037O11+D0
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I732406/annotation1944475/range1944475/1

https://synbiohub.org/public/igem/BBa_I732406/annotation1944475/range1944475/1
type: Range
persistent identity: range1944475
display id: range1944475
version: 1
direction: direction/forward
top level: U037O11+D0
start: 1122
end: 1162
orientation: inline

START
type: SequenceAnnotation
persistent identity: annotation1944465
display id: annotation1944465
version: 1
top level: U037O11+D0
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_I732406/annotation1944465/range1944465/1

https://synbiohub.org/public/igem/BBa_I732406/annotation1944465/range1944465/1
type: Range
persistent identity: range1944465
display id: range1944465
version: 1
direction: direction/forward
top level: U037O11+D0
start: 46
end: 48
orientation: inline

BBa_I732006
type: SequenceAnnotation
persistent identity: annotation1944468
display id: annotation1944468
version: 1
top level: U037O11+D0
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I732406/annotation1944468/range1944468/1

https://synbiohub.org/public/igem/BBa_I732406/annotation1944468/range1944468/1
type: Range
persistent identity: range1944468
display id: range1944468
version: 1
direction: direction/reverse
top level: U037O11+D0
start: 46
end: 279
orientation: inline

lacZ
type: SequenceAnnotation
persistent identity: annotation1944466
display id: annotation1944466
version: 1
top level: U037O11+D0
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_I732406/annotation1944466/range1944466/1

https://synbiohub.org/public/igem/BBa_I732406/annotation1944466/range1944466/1
type: Range
persistent identity: range1944466
display id: range1944466
version: 1
direction: direction/forward
top level: U037O11+D0
start: 46
end: 279
orientation: inline

Pnot(O46)
type: ComponentDefinition
persistent identity: BBa I732277
display id: BBa_I732277
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member with Hybrid Operator (D001O46)
creator: Ma Rui
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:57Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732277_sequence/1

BBa_K112708
type: ComponentDefinition
persistent identity: BBa K112708
display id: BBa_K112708
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PfhuA
creator: Bing Xia
created: 2008-10-20T11:00:00Z
modified: 2015-05-08T01:09:18Z
discontinued: false
dominant: false
group u list: _224_
m user id: 0
owner id: 2998
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: The fhuA promoter. ---- This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at: [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
mutable provenance: Construction of <PfhuA> basic part K112708 PCR of dv019/dv020 on MG1655 (225bp, EcoRI/BamHI/DpnI) Sub into pBca1256 (EcoRI/BamHI, 2472+225, L) Product is pBca1256-K112708 ----------------------------------------------- dv019 Forward biobricking of <PfhuA> cgataGAATTCatgAGATCTgctaagcgtgaaataccggatgg dv020 Reverse biobricking of <PfhuA> cgttaGGATCCactctgatgtaaagtgaatgataacg
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K112708_sequence/1

BBa_I746104
type: ComponentDefinition
persistent identity: BBa I746104
display id: BBa_I746104
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: P2 promoter in agr operon from S. aureus
creator: Zhizhen Zhao
created: 2007-08-30T11:00:00Z
modified: 2015-08-31T04:08:04Z
discontinued: false
dominant: false
experience: experience/None
group u list: _116_
m user id: 0
owner id: 2121
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: The agr P2 operon is an autocatalytic sensory transduction system in Staphylococcus aureus. P2 promoter regulates the synthesis of AgrB (a transmembrane protein) and AgrD (precursor of AIP autoinducing peptide). AIP binds to AgrC, a membrane binding receptor and it phosphorylates AgrA. The phosphorylated AgrA increases the expression level from P2 promoter.
mutable notes: Codon usage has been optimised for E. coli (which also seems to give good results for B. subtilis
mutable provenance: The section of the sequence constituting the P2 promoter is subject to rather more debate than the sequences of the coding regions. [http://jb.asm.org/cgi/content/full/186/22/7549 Koenig, Robbin L., Ray, Jessica L., Maleki, Soheila J., Smeltzer, Mark S., Hurlburt, Barry K. Staphylococcus aureus AgrA Binding to the RNAIII-agr Regulatory Region J. Bacteriol. 2004 186: 7549-7555] shows the areas of the sequence to which phosphorylated AgrA binds while L Rao, R K Karls, and M J Betley: In vitro transcription of pathogenesis-related genes by purified RNA polymerase from Staphylococcus aureus. Bacteriol. 1995 May; 177(10): 2609???2614. shows the transcription start sites; we took the overall extent of the promoter region from that labelled in Morfeldt, E., K. Tegmark, and S. Arvidson. 1996. Transcriptional control of the agr-dependent virulence gene regulator, RNAIII, in Staphylococcus aureus. Mol. Microbiol. 21:1227???1237. (not available online).
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I746104_sequence/1

Pnot(O22)
type: ComponentDefinition
persistent identity: BBa I732202
display id: BBa_I732202
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: NOT Gate Promoter Family Member (D001O22)
creator: Zhan Jian
created: 2007-08-11T11:00:00Z
modified: 2015-08-31T04:07:57Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732202_sequence/1

BBa_K137047
type: ComponentDefinition
persistent identity: BBa K137047
display id: BBa_K137047
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: 250 bp inverted tetR promoter
creator: Allen Lin
created: 2008-07-20T11:00:00Z
modified: 2015-05-08T01:10:09Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _187_
m user id: 0
owner id: 3112
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Inverted tetR promoter with noncoding, spacer DNA downstream of it to make the total length 250 bp. The promoter faces the 5' direction.
mutable notes: This part is in a series of inverted tetR promoters that have total lengths of 150, 250, 350, 450, 650, and 850 bp. We chose the region upstream of tetR in part Q04400 to be the noncoding DNA segment. This noncoding segment consists of the 3' end of tetR and B0015. None of the parts in this series was long enough to include the start codon of tetR, so a functional tetR protein should not be transcribed.
mutable provenance: Primers were synthesized to bind to part Q04400, and then a PCR reaction was run.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: tetR promoter
sequence: https://synbiohub.org/public/igem/BBa_K137047_sequence/1

tetR promoter
type: SequenceAnnotation
persistent identity: annotation1968020
display id: annotation1968020
version: 1
top level: BBa_K137047
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K137047/annotation1968020/range1968020/1

https://synbiohub.org/public/igem/BBa_K137047/annotation1968020/range1968020/1
type: Range
persistent identity: range1968020
display id: range1968020
version: 1
direction: direction/reverse
top level: BBa_K137047
start: 1
end: 54
orientation: inline

BBa_K091111
type: ComponentDefinition
persistent identity: BBa K091111
display id: BBa_K091111
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LacIQ promoter
creator: Robert Cool
created: 2008-06-19T11:00:00Z
modified: 2015-05-08T01:08:37Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _191_
m user id: 0
owner id: 3080
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This is a modified version of the LacIQ promoter tha should bind LacIQ tighter than the original.
mutable notes: no special considerations
mutable provenance: This is a modified version of the LacIQ promoter
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -10
sequence annotation: -35
sequence: https://synbiohub.org/public/igem/BBa_K091111_sequence/1

-10
type: SequenceAnnotation
persistent identity: annotation1964080
display id: annotation1964080
version: 1
top level: BBa_K091111
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K091111/annotation1964080/range1964080/1

https://synbiohub.org/public/igem/BBa_K091111/annotation1964080/range1964080/1
type: Range
persistent identity: range1964080
display id: range1964080
version: 1
direction: direction/forward
top level: BBa_K091111
start: 44
end: 49
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1964079
display id: annotation1964079
version: 1
top level: BBa_K091111
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K091111/annotation1964079/range1964079/1

https://synbiohub.org/public/igem/BBa_K091111/annotation1964079/range1964079/1
type: Range
persistent identity: range1964079
display id: range1964079
version: 1
direction: direction/forward
top level: BBa_K091111
start: 17
end: 22
orientation: inline

BBa_J23108
type: ComponentDefinition
persistent identity: BBa J23108
display id: BBa_J23108
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: constitutive promoter family member
creator: John Anderson
created: 2006-08-16T11:00:00Z
modified: 2015-08-31T04:08:40Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: true
mutable description: Later
mutable notes: N/A
mutable provenance: Later
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J23108_sequence/1

Pnot(O12)
type: ComponentDefinition
persistent identity: BBa I732270
display id: BBa_I732270
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member with Hybrid Operator (D001O12)
creator: Zhan Jian
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:57Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732270_sequence/1

BBa_I732452
type: ComponentDefinition
persistent identity: BBa I732452
display id: BBa_I732452
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member (U073O26+D062O61)
creator: Zhan Jian
created: 2007-10-16T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732452_sequence/1

BBa_K1520026
type: ComponentDefinition
persistent identity: BBa K1520026
display id: BBa_K1520026
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ptet-rbs-rfp-Ter-Plac-rbs-tetR-Ter-Pcons2-rbs-lacI-Ter
creator: yue wu
created: 2014-09-21T11:00:00Z
modified: 2015-05-08T01:10:49Z
discontinued: false
dominant: false
group u list: _1902_
m user id: 0
owner id: 21318
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Ptet-rbs-rfp-Ter-Plac-rbs-tetR-Ter-Pcons2-rbs-lacI-Ter. It produces rfp when there is less lactose.
mutable notes: None
mutable provenance: Ptet, rfp, Plac, tetR, Pcons2, lacI, Ter
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1520025
component: BBa_K1520008
sequence annotation: BBa_K1520008
sequence annotation: BBa_K1520025
sequence: https://synbiohub.org/public/igem/BBa_K1520026_sequence/1

BBa_K1520025
type: Component
persistent identity: component2385313
display id: component2385313
version: 1
top level: BBa_K1520026
access: public
definition: BBa_K1520025

BBa_K1520008
type: Component
persistent identity: component2385319
display id: component2385319
version: 1
top level: BBa_K1520026
access: public
definition: https://synbiohub.org/public/igem/BBa_K1520008/1

BBa_K1520008
type: SequenceAnnotation
persistent identity: annotation2385319
display id: annotation2385319
version: 1
top level: BBa_K1520026
location: https://synbiohub.org/public/igem/BBa_K1520026/annotation2385319/range2385319/1
component: BBa_K1520008

https://synbiohub.org/public/igem/BBa_K1520026/annotation2385319/range2385319/1
type: Range
persistent identity: range2385319
display id: range2385319
version: 1
direction: direction/reverse
top level: BBa_K1520026
start: 1996
end: 3346
orientation: inline

BBa_K1520025
type: SequenceAnnotation
persistent identity: annotation2385313
display id: annotation2385313
version: 1
top level: BBa_K1520026
location: https://synbiohub.org/public/igem/BBa_K1520026/annotation2385313/range2385313/1
component: BBa_K1520025

https://synbiohub.org/public/igem/BBa_K1520026/annotation2385313/range2385313/1
type: Range
persistent identity: range2385313
display id: range2385313
version: 1
direction: direction/reverse
top level: BBa_K1520026
start: 1
end: 1987
orientation: inline

BBa_J22052
type: ComponentDefinition
persistent identity: BBa J22052
display id: BBa_J22052
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Pcya
creator: Sugat Dabholkar
created: 2006-09-03T11:00:00Z
modified: 2015-08-31T04:08:38Z
discontinued: false
dominant: false
group u list: _70_44_
m user id: 0
owner id: 439
owning group id: 70
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is a adenylate cyclase promoter that has been reported to oscillate in cell cycle dependant manner.
mutable notes: No design problems
mutable provenance: Ref: J. Biol. Chem., Vol. 260, Issue 5, 3063-3070, 03, 1985 Transcription of the Escherichia coli adenylate cyclase gene is negatively regulated by cAMP-cAMP receptor protein H Aiba [bases -53 to 12]
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J22052_sequence/1

BBa_K112118
type: ComponentDefinition
persistent identity: BBa K112118
display id: BBa_K112118
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: rrnB P1 promoter
creator: Bing Xia
created: 2008-10-20T11:00:00Z
modified: 2015-05-08T01:09:14Z
discontinued: false
dominant: false
group u list: _224_
m user id: 0
owner id: 2998
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: ---- This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at: [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
mutable provenance: PCR mv028/mv029 on rrnB native sequence (721bp, EcoRI/BamHI/DpnI) Sub into pBca1256 (EcoRI/BamHI, 2472+910, L) Product is pBca1256-K112118 ----------------------------------------------- mv028 Forward Biobricking of rrnB P1 promoter cgataGAATTCatgAGATCTatgcactaaaacgtatcctacgcccg mv029 Reverse Biobricking of rrnB P1 promoter cgttaGGATCCcgccttcccgctacagagtcaagc
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K112118_sequence/1

Las CIO
type: ComponentDefinition
persistent identity: BBa I14016
display id: BBa_I14016
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: P(Las) CIO
creator: Vikram Vijayan, Allen Hsu, Lawrence Fomundam
created: 2004-07-29T11:00:00Z
modified: 2015-08-31T04:07:37Z
discontinued: false
dominant: false
experience: experience/None
group u list: _4_
m user id: 0
owner id: 171
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: LasR and 3OC12HSL regulated promoter with CI Operator 1 (binding site for lambda cI repressor)
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -10
sequence annotation: R0079
sequence annotation: -35
sequence annotation: CI lambda O1
sequence: https://synbiohub.org/public/igem/BBa_I14016_sequence/1

-10
type: SequenceAnnotation
persistent identity: annotation1026125
display id: annotation1026125
version: 1
top level: Las CIO
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I14016/annotation1026125/range1026125/1

https://synbiohub.org/public/igem/BBa_I14016/annotation1026125/range1026125/1
type: Range
persistent identity: range1026125
display id: range1026125
version: 1
direction: direction/forward
top level: Las CIO
start: 140
end: 145
orientation: inline

R0079
type: SequenceAnnotation
persistent identity: annotation1017859
display id: annotation1017859
version: 1
top level: Las CIO
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I14016/annotation1017859/range1017859/1

https://synbiohub.org/public/igem/BBa_I14016/annotation1017859/range1017859/1
type: Range
persistent identity: range1017859
display id: range1017859
version: 1
direction: direction/forward
top level: Las CIO
start: 1
end: 151
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1026124
display id: annotation1026124
version: 1
top level: Las CIO
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I14016/annotation1026124/range1026124/1

https://synbiohub.org/public/igem/BBa_I14016/annotation1026124/range1026124/1
type: Range
persistent identity: range1026124
display id: range1026124
version: 1
direction: direction/forward
top level: Las CIO
start: 117
end: 122
orientation: inline

CI lambda O1
type: SequenceAnnotation
persistent identity: annotation1017878
display id: annotation1017878
version: 1
top level: Las CIO
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I14016/annotation1017878/range1017878/1

https://synbiohub.org/public/igem/BBa_I14016/annotation1017878/range1017878/1
type: Range
persistent identity: range1017878
display id: range1017878
version: 1
direction: direction/forward
top level: Las CIO
start: 152
end: 168
orientation: inline

BBa_K266007
type: ComponentDefinition
persistent identity: BBa K266007
display id: BBa_K266007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Complex QS -> LuxI & LasI circuit
creator: UNAM FCIENCIAS-CINVESTAV IRA
created: 2009-10-12T11:00:00Z
modified: 2015-05-08T01:11:43Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _363_
m user id: 0
owner id: 1955
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: Complex Quorum sensing circuit that receives the signal of PAI+LasR and AI+LuxR to control the production of LuxI and LasI enzymes.
mutable notes: None
mutable provenance: The biobricks BBa_K266006 and BBa_K266000 where used to build this biobrick
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Signalling
role: SO:0000804
component: BBa_K266000
component: BBa_K266006
sequence annotation: BBa_K266000
sequence annotation: BBa_K266006
sequence: https://synbiohub.org/public/igem/BBa_K266007_sequence/1

BBa_K266000
type: Component
persistent identity: component2297452
display id: component2297452
version: 1
top level: BBa_K266007
access: public
definition: BBa_K266000

BBa_K266006
type: Component
persistent identity: component2297433
display id: component2297433
version: 1
top level: BBa_K266007
access: public
definition: BBa_K266006

BBa_K266000
type: SequenceAnnotation
persistent identity: annotation2297452
display id: annotation2297452
version: 1
top level: BBa_K266007
location: https://synbiohub.org/public/igem/BBa_K266007/annotation2297452/range2297452/1
component: BBa_K266000

https://synbiohub.org/public/igem/BBa_K266007/annotation2297452/range2297452/1
type: Range
persistent identity: range2297452
display id: range2297452
version: 1
direction: direction/reverse
top level: BBa_K266007
start: 1714
end: 2676
orientation: inline

BBa_K266006
type: SequenceAnnotation
persistent identity: annotation2297433
display id: annotation2297433
version: 1
top level: BBa_K266007
location: https://synbiohub.org/public/igem/BBa_K266007/annotation2297433/range2297433/1
component: BBa_K266006

https://synbiohub.org/public/igem/BBa_K266007/annotation2297433/range2297433/1
type: Range
persistent identity: range2297433
display id: range2297433
version: 1
direction: direction/reverse
top level: BBa_K266007
start: 1
end: 1705
orientation: inline

BBa_K256033
type: ComponentDefinition
persistent identity: BBa K256033
display id: BBa_K256033
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Infrared signal reporter (J23119:IFP:J23119:HO1)
creator: iGEM09_NTU-Singapore
created: 2009-10-19T11:00:00Z
modified: 2015-05-08T01:11:41Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _359_
m user id: 0
owner id: 4864
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This part co-expresses IFP and HO1.
mutable notes: --
mutable provenance: Gene sequence is obtained directly from Tsien's lab.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_K256018
component: BBa_K256020
sequence annotation: BBa_K256018
sequence annotation: BBa_K256020
sequence: https://synbiohub.org/public/igem/BBa_K256033_sequence/1

BBa_K256018
type: Component
persistent identity: component2288200
display id: component2288200
version: 1
top level: BBa_K256033
access: public
definition: BBa_K256018

BBa_K256020
type: Component
persistent identity: component2288213
display id: component2288213
version: 1
top level: BBa_K256033
access: public
definition: BBa_K256020

BBa_K256018
type: SequenceAnnotation
persistent identity: annotation2288200
display id: annotation2288200
version: 1
top level: BBa_K256033
location: https://synbiohub.org/public/igem/BBa_K256033/annotation2288200/range2288200/1
component: BBa_K256018

https://synbiohub.org/public/igem/BBa_K256033/annotation2288200/range2288200/1
type: Range
persistent identity: range2288200
display id: range2288200
version: 1
direction: direction/reverse
top level: BBa_K256033
start: 1
end: 1167
orientation: inline

BBa_K256020
type: SequenceAnnotation
persistent identity: annotation2288213
display id: annotation2288213
version: 1
top level: BBa_K256033
location: https://synbiohub.org/public/igem/BBa_K256033/annotation2288213/range2288213/1
component: BBa_K256020

https://synbiohub.org/public/igem/BBa_K256033/annotation2288213/range2288213/1
type: Range
persistent identity: range2288213
display id: range2288213
version: 1
direction: direction/reverse
top level: BBa_K256033
start: 1176
end: 2124
orientation: inline

BBa_J23103
type: ComponentDefinition
persistent identity: BBa J23103
display id: BBa_J23103
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: constitutive promoter family member
creator: John Anderson
created: 2006-08-03T11:00:00Z
modified: 2015-08-31T04:08:40Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: replace later
mutable notes: N/A
mutable provenance: isolated from library of promoters
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J23103_sequence/1

U085O11+D0
type: ComponentDefinition
persistent identity: BBa I732424
display id: BBa_I732424
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member (U085O11+D062O22)
creator: Zhan Jian
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732424_sequence/1

BBa_J23105
type: ComponentDefinition
persistent identity: BBa J23105
display id: BBa_J23105
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: constitutive promoter family member
creator: John Anderson
created: 2006-08-13T11:00:00Z
modified: 2015-08-31T04:08:40Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: true
mutable description: Later
mutable notes: N/A
mutable provenance: Isolated from library of promoters
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J23105_sequence/1

BBa_K180010
type: ComponentDefinition
persistent identity: BBa K180010
display id: BBa_K180010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Rock-paper-scissors - Rock primary plasmid
creator: Richard Wilson, Stephanie Chambers
created: 2009-10-20T11:00:00Z
modified: 2015-05-08T01:11:06Z
discontinued: false
dominant: false
experience: experience/None
group u list: _278_
m user id: 0
owner id: 4082
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: Composite part, consisting of BBa_K180011 and BBa_K180012, but with BBa_K180011 reading along reverse strand, and BBa_K180012 reading along the forward strand. Forms the 'rock' primary plasmid in Team:Southampton's rock-paper-scissors project design.
mutable notes: This part has been specified as a basic BioBrick, due to some of the component parts reading in reverse (unable to specify using the component BioBrick format).
mutable provenance: See relevant biobrick component parts (BBa_K180011 and BBa_K180012).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: rhlR
sequence annotation: conserved
sequence annotation: K180008
sequence annotation: C0171
sequence annotation: Ptac
sequence annotation: J23119
sequence annotation: lacIq
sequence annotation: lasI
sequence annotation: -10
sequence annotation: B0034
sequence annotation: B0015
sequence annotation: K180012
sequence annotation: -35
sequence annotation: mCherry
sequence annotation: K180000
sequence annotation: C0178
sequence annotation: K180011
sequence: https://synbiohub.org/public/igem/BBa_K180010_sequence/1

rhlR
type: SequenceAnnotation
persistent identity: annotation2058312
display id: annotation2058312
version: 1
top level: BBa_K180010
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K180010/annotation2058312/range2058312/1

https://synbiohub.org/public/igem/BBa_K180010/annotation2058312/range2058312/1
type: Range
persistent identity: range2058312
display id: range2058312
version: 1
direction: direction/reverse
top level: BBa_K180010
start: 138
end: 866
orientation: inline

conserved
type: SequenceAnnotation
persistent identity: annotation2058300
display id: annotation2058300
version: 1
top level: BBa_K180010
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K180010/annotation2058300/range2058300/1

https://synbiohub.org/public/igem/BBa_K180010/annotation2058300/range2058300/1
type: Range
persistent identity: range2058300
display id: range2058300
version: 1
direction: direction/forward
top level: BBa_K180010
start: 2395
end: 2398
orientation: inline

K180008
type: SequenceAnnotation
persistent identity: annotation2058310
display id: annotation2058310
version: 1
top level: BBa_K180010
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K180010/annotation2058310/range2058310/1

https://synbiohub.org/public/igem/BBa_K180010/annotation2058310/range2058310/1
type: Range
persistent identity: range2058310
display id: range2058310
version: 1
direction: direction/forward
top level: BBa_K180010
start: 2409
end: 3116
orientation: inline

C0171
type: SequenceAnnotation
persistent identity: annotation2058289
display id: annotation2058289
version: 1
top level: BBa_K180010
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K180010/annotation2058289/range2058289/1

https://synbiohub.org/public/igem/BBa_K180010/annotation2058289/range2058289/1
type: Range
persistent identity: range2058289
display id: range2058289
version: 1
direction: direction/reverse
top level: BBa_K180010
start: 138
end: 866
orientation: inline

Ptac
type: SequenceAnnotation
persistent identity: annotation2058309
display id: annotation2058309
version: 1
top level: BBa_K180010
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K180010/annotation2058309/range2058309/1

https://synbiohub.org/public/igem/BBa_K180010/annotation2058309/range2058309/1
type: Range
persistent identity: range2058309
display id: range2058309
version: 1
direction: direction/forward
top level: BBa_K180010
start: 2260
end: 2287
orientation: inline

J23119
type: SequenceAnnotation
persistent identity: annotation2058304
display id: annotation2058304
version: 1
top level: BBa_K180010
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K180010/annotation2058304/range2058304/1

https://synbiohub.org/public/igem/BBa_K180010/annotation2058304/range2058304/1
type: Range
persistent identity: range2058304
display id: range2058304
version: 1
direction: direction/reverse
top level: BBa_K180010
start: 893
end: 927
orientation: inline

conserved
type: SequenceAnnotation
persistent identity: annotation2058303
display id: annotation2058303
version: 1
top level: BBa_K180010
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K180010/annotation2058303/range2058303/1

https://synbiohub.org/public/igem/BBa_K180010/annotation2058303/range2058303/1
type: Range
persistent identity: range2058303
display id: range2058303
version: 1
direction: direction/reverse
top level: BBa_K180010
start: 877
end: 880
orientation: inline

lacIq
type: SequenceAnnotation
persistent identity: annotation2058308
display id: annotation2058308
version: 1
top level: BBa_K180010
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K180010/annotation2058308/range2058308/1

https://synbiohub.org/public/igem/BBa_K180010/annotation2058308/range2058308/1
type: Range
persistent identity: range2058308
display id: range2058308
version: 1
direction: direction/forward
top level: BBa_K180010
start: 936
end: 2017
orientation: inline

lasI
type: SequenceAnnotation
persistent identity: annotation2058314
display id: annotation2058314
version: 1
top level: BBa_K180010
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K180010/annotation2058314/range2058314/1

https://synbiohub.org/public/igem/BBa_K180010/annotation2058314/range2058314/1
type: Range
persistent identity: range2058314
display id: range2058314
version: 1
direction: direction/forward
top level: BBa_K180010
start: 3143
end: 3751
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2058306
display id: annotation2058306
version: 1
top level: BBa_K180010
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K180010/annotation2058306/range2058306/1

https://synbiohub.org/public/igem/BBa_K180010/annotation2058306/range2058306/1
type: Range
persistent identity: range2058306
display id: range2058306
version: 1
direction: direction/reverse
top level: BBa_K180010
start: 899
end: 904
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation2058302
display id: annotation2058302
version: 1
top level: BBa_K180010
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K180010/annotation2058302/range2058302/1

https://synbiohub.org/public/igem/BBa_K180010/annotation2058302/range2058302/1
type: Range
persistent identity: range2058302
display id: range2058302
version: 1
direction: direction/reverse
top level: BBa_K180010
start: 873
end: 884
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation2058292
display id: annotation2058292
version: 1
top level: BBa_K180010
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K180010/annotation2058292/range2058292/1

https://synbiohub.org/public/igem/BBa_K180010/annotation2058292/range2058292/1
type: Range
persistent identity: range2058292
display id: range2058292
version: 1
direction: direction/forward
top level: BBa_K180010
start: 3125
end: 3136
orientation: inline

B0015
type: SequenceAnnotation
persistent identity: annotation2058315
display id: annotation2058315
version: 1
top level: BBa_K180010
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K180010/annotation2058315/range2058315/1

https://synbiohub.org/public/igem/BBa_K180010/annotation2058315/range2058315/1
type: Range
persistent identity: range2058315
display id: range2058315
version: 1
direction: direction/forward
top level: BBa_K180010
start: 3760
end: 3888
orientation: inline

K180012
type: SequenceAnnotation
persistent identity: annotation2058287
display id: annotation2058287
version: 1
top level: BBa_K180010
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K180010/annotation2058287/range2058287/1

https://synbiohub.org/public/igem/BBa_K180010/annotation2058287/range2058287/1
type: Range
persistent identity: range2058287
display id: range2058287
version: 1
direction: direction/forward
top level: BBa_K180010
start: 936
end: 3888
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2058305
display id: annotation2058305
version: 1
top level: BBa_K180010
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K180010/annotation2058305/range2058305/1

https://synbiohub.org/public/igem/BBa_K180010/annotation2058305/range2058305/1
type: Range
persistent identity: range2058305
display id: range2058305
version: 1
direction: direction/reverse
top level: BBa_K180010
start: 922
end: 927
orientation: inline

B0015
type: SequenceAnnotation
persistent identity: annotation2058288
display id: annotation2058288
version: 1
top level: BBa_K180010
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K180010/annotation2058288/range2058288/1

https://synbiohub.org/public/igem/BBa_K180010/annotation2058288/range2058288/1
type: Range
persistent identity: range2058288
display id: range2058288
version: 1
direction: direction/reverse
top level: BBa_K180010
start: 1
end: 129
orientation: inline

mCherry
type: SequenceAnnotation
persistent identity: annotation2058311
display id: annotation2058311
version: 1
top level: BBa_K180010
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K180010/annotation2058311/range2058311/1

https://synbiohub.org/public/igem/BBa_K180010/annotation2058311/range2058311/1
type: Range
persistent identity: range2058311
display id: range2058311
version: 1
direction: direction/forward
top level: BBa_K180010
start: 2409
end: 3116
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation2058291
display id: annotation2058291
version: 1
top level: BBa_K180010
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K180010/annotation2058291/range2058291/1

https://synbiohub.org/public/igem/BBa_K180010/annotation2058291/range2058291/1
type: Range
persistent identity: range2058291
display id: range2058291
version: 1
direction: direction/forward
top level: BBa_K180010
start: 2391
end: 2402
orientation: inline

conserved
type: SequenceAnnotation
persistent identity: annotation2058301
display id: annotation2058301
version: 1
top level: BBa_K180010
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K180010/annotation2058301/range2058301/1

https://synbiohub.org/public/igem/BBa_K180010/annotation2058301/range2058301/1
type: Range
persistent identity: range2058301
display id: range2058301
version: 1
direction: direction/forward
top level: BBa_K180010
start: 3129
end: 3132
orientation: inline

K180000
type: SequenceAnnotation
persistent identity: annotation2058307
display id: annotation2058307
version: 1
top level: BBa_K180010
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K180010/annotation2058307/range2058307/1

https://synbiohub.org/public/igem/BBa_K180010/annotation2058307/range2058307/1
type: Range
persistent identity: range2058307
display id: range2058307
version: 1
direction: direction/forward
top level: BBa_K180010
start: 936
end: 2382
orientation: inline

C0178
type: SequenceAnnotation
persistent identity: annotation2058313
display id: annotation2058313
version: 1
top level: BBa_K180010
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K180010/annotation2058313/range2058313/1

https://synbiohub.org/public/igem/BBa_K180010/annotation2058313/range2058313/1
type: Range
persistent identity: range2058313
display id: range2058313
version: 1
direction: direction/forward
top level: BBa_K180010
start: 3143
end: 3751
orientation: inline

K180011
type: SequenceAnnotation
persistent identity: annotation2058286
display id: annotation2058286
version: 1
top level: BBa_K180010
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K180010/annotation2058286/range2058286/1

https://synbiohub.org/public/igem/BBa_K180010/annotation2058286/range2058286/1
type: Range
persistent identity: range2058286
display id: range2058286
version: 1
direction: direction/reverse
top level: BBa_K180010
start: 1
end: 927
orientation: inline

BBa_K292001
type: ComponentDefinition
persistent identity: BBa K292001
display id: BBa_K292001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Double terminator + Constitutive promoter + Strong RBS
creator: David Charoy
created: 2009-10-17T11:00:00Z
modified: 2015-05-08T01:11:49Z
discontinued: false
dominant: false
group u list: _394_
m user id: 0
owner id: 5212
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part contains a double terminator and a constitutive prokaryotic promoter and a strong RBS (BBa_B0014 + BBa_J23100 + BBa_B0030). It is a simple promoter in the goals to begin gene transcription. For Sup???biotech-Paris (2009) team this part is used when adding to a recombinant lambda phage and to begin the transcription of synthetic genes inside the Lambda phage genome.
mutable notes: N/A
mutable provenance: BBa_B0014 : Designed by Reshma Shetty Group: Registry (2003-07-16). BBa_J23100 : Designed by John Anderson Group: iGEM2006_Berkeley (2006-08-04.) BBa_B0030 : Designed by Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. Group: NCBS, Prather Lab.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_J23100
component: BBa_B0011
component: BBa_B0012
component: BBa_B0030
sequence annotation: BBa_J23100
sequence annotation: BBa_B0011
sequence annotation: BBa_B0012
sequence annotation: BBa_B0030
sequence: https://synbiohub.org/public/igem/BBa_K292001_sequence/1

BBa_J23100
type: Component
persistent identity: component2052039
display id: component2052039
version: 1
top level: BBa_K292001
access: public
definition: BBa_J23100

BBa_B0011
type: Component
persistent identity: component2052037
display id: component2052037
version: 1
top level: BBa_K292001
access: public
definition: https://synbiohub.org/public/igem/BBa_B0011/1

BBa_B0012
type: Component
persistent identity: component2052033
display id: component2052033
version: 1
top level: BBa_K292001
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0030
type: Component
persistent identity: component2052041
display id: component2052041
version: 1
top level: BBa_K292001
access: public
definition: https://synbiohub.org/public/igem/BBa_B0030/1

BBa_J23100
type: SequenceAnnotation
persistent identity: annotation2052039
display id: annotation2052039
version: 1
top level: BBa_K292001
location: https://synbiohub.org/public/igem/BBa_K292001/annotation2052039/range2052039/1
component: BBa_J23100

https://synbiohub.org/public/igem/BBa_K292001/annotation2052039/range2052039/1
type: Range
persistent identity: range2052039
display id: range2052039
version: 1
direction: direction/reverse
top level: BBa_K292001
start: 104
end: 138
orientation: inline

BBa_B0011
type: SequenceAnnotation
persistent identity: annotation2052037
display id: annotation2052037
version: 1
top level: BBa_K292001
location: https://synbiohub.org/public/igem/BBa_K292001/annotation2052037/range2052037/1
component: BBa_B0011

https://synbiohub.org/public/igem/BBa_K292001/annotation2052037/range2052037/1
type: Range
persistent identity: range2052037
display id: range2052037
version: 1
direction: direction/forward
top level: BBa_K292001
start: 50
end: 95
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2052033
display id: annotation2052033
version: 1
top level: BBa_K292001
location: https://synbiohub.org/public/igem/BBa_K292001/annotation2052033/range2052033/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K292001/annotation2052033/range2052033/1
type: Range
persistent identity: range2052033
display id: range2052033
version: 1
direction: direction/forward
top level: BBa_K292001
start: 1
end: 41
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation2052041
display id: annotation2052041
version: 1
top level: BBa_K292001
location: https://synbiohub.org/public/igem/BBa_K292001/annotation2052041/range2052041/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_K292001/annotation2052041/range2052041/1
type: Range
persistent identity: range2052041
display id: range2052041
version: 1
direction: direction/forward
top level: BBa_K292001
start: 147
end: 161
orientation: inline

BBa_K094120
type: ComponentDefinition
persistent identity: BBa K094120
display id: BBa_K094120
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pLacI/ara-1
creator: Shi Lei
created: 2008-10-12T11:00:00Z
modified: 2015-05-08T01:08:40Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _255_
m user id: 0
owner id: 2589
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This promoter can be repressed by lacI protein and induced by either IPTG or arabinose.
mutable notes: IPTG has better effect on this promoter than arabinose
mutable provenance: Standard pLacI/ara-1 promoter
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: pLac/ara-1
sequence: https://synbiohub.org/public/igem/BBa_K094120_sequence/1

pLac/ara-1
type: SequenceAnnotation
persistent identity: annotation1980631
display id: annotation1980631
version: 1
top level: BBa_K094120
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K094120/annotation1980631/range1980631/1

https://synbiohub.org/public/igem/BBa_K094120/annotation1980631/range1980631/1
type: Range
persistent identity: range1980631
display id: range1980631
version: 1
direction: direction/forward
top level: BBa_K094120
start: 1
end: 103
orientation: inline

BBa_K117002
type: ComponentDefinition
persistent identity: BBa K117002
display id: BBa_K117002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LsrA promoter (indirectly activated by AI-2)
creator: Nguyen Xuan Hung
created: 2008-10-06T11:00:00Z
modified: 2015-05-08T01:09:34Z
discontinued: false
dominant: false
experience: experience/None
group u list: _209_
m user id: 0
owner id: 2774
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: The regulatory network for the uptake of Escherichia coli autoinducer 2 (AI-2) is comprised of a transporter complex, LsrABCD; its repressor, LsrR; and a cognate signal kinase, LsrK. This network is an integral part of the AI-2 quorum-sensing (QS) system. Once uptaken into the cell, AI-2 will be phosphorylated under the effect of LsrK. Without the presence of AI-2, LsrA promoter is inhibited by the repressor, LsrR. Phosphorylated AI-2 will prevent LsrR from inhibiting this promoter, hence activating it so that the downstream sequence can be expressed. To prevent the cell from self-inducing this promoter, we must suppress its AI-2 producing capability. It can be done by knocking out LuxS, one of the vital gene required for producing AI-2. By doing that, LsrA promoter can only be activated under the presence of AI-2 from other sources than the host cell which is LuxS mutant (say, by contact with normal cells). More information on AI-2 quorum-sensing (QS) system please refer to: http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=1952038
mutable notes: to be updated
mutable provenance: to be updated
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: RBS BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K117002_sequence/1

RBS BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1979372
display id: annotation1979372
version: 1
top level: BBa_K117002
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K117002/annotation1979372/range1979372/1

https://synbiohub.org/public/igem/BBa_K117002/annotation1979372/range1979372/1
type: Range
persistent identity: range1979372
display id: range1979372
version: 1
direction: direction/forward
top level: BBa_K117002
start: 112
end: 123
orientation: inline

P(Lac) IQ
type: ComponentDefinition
persistent identity: BBa I14032
display id: BBa_I14032
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: promoter P(Lac) IQ
creator: Vikram Vijayan, Allen Hsu, Lawrence Fomundam
created: 2004-08-03T11:00:00Z
modified: 2015-08-31T04:07:37Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _4_
m user id: 0
owner id: 171
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Constitutive Promoter, High Transcription
mutable provenance: Plasmid pMAL-p2X
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -10
sequence annotation: P(Lac) IQ
sequence annotation: -35
sequence: https://synbiohub.org/public/igem/BBa_I14032_sequence/1

-10
type: SequenceAnnotation
persistent identity: annotation1028344
display id: annotation1028344
version: 1
top level: P(Lac) IQ
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I14032/annotation1028344/range1028344/1

https://synbiohub.org/public/igem/BBa_I14032/annotation1028344/range1028344/1
type: Range
persistent identity: range1028344
display id: range1028344
version: 1
direction: direction/forward
top level: P(Lac) IQ
start: 26
end: 31
orientation: inline

P(Lac) IQ
type: SequenceAnnotation
persistent identity: annotation1028342
display id: annotation1028342
version: 1
top level: P(Lac) IQ
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_I14032/annotation1028342/range1028342/1

https://synbiohub.org/public/igem/BBa_I14032/annotation1028342/range1028342/1
type: Range
persistent identity: range1028342
display id: range1028342
version: 1
direction: direction/forward
top level: P(Lac) IQ
start: 1
end: 37
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1028343
display id: annotation1028343
version: 1
top level: P(Lac) IQ
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I14032/annotation1028343/range1028343/1

https://synbiohub.org/public/igem/BBa_I14032/annotation1028343/range1028343/1
type: Range
persistent identity: range1028343
display id: range1028343
version: 1
direction: direction/forward
top level: P(Lac) IQ
start: 3
end: 8
orientation: inline

U073O11+D0
type: ComponentDefinition
persistent identity: BBa I732429
display id: BBa_I732429
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member (U073O11+D050O22)
creator: Zhan Jian
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732429_sequence/1

Pnot(O47)
type: ComponentDefinition
persistent identity: BBa I732278
display id: BBa_I732278
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member with Hybrid Operator (D001O47)
creator: Ma Rui
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:57Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732278_sequence/1

BBa_R2111
type: ComponentDefinition
persistent identity: BBa R2111
display id: BBa_R2111
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter with operator site for C2003
creator: Reshma Shetty
created: 2006-10-20T11:00:00Z
modified: 2015-05-08T01:14:16Z
discontinued: false
dominant: false
group u list: _41_
m user id: 0
owner id: 126
owning group id: 70
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This promoter is designed to bind to C2003.
mutable notes: The placement of the operator was designed to be near optimal for steric interference between C2003 and RNA polymerase.
mutable provenance: This promoter was rationally designed.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: transcription start site
sequence annotation: C2003 operator site
sequence annotation: -10 site
sequence annotation: -35 site
sequence: https://synbiohub.org/public/igem/BBa_R2111_sequence/1

transcription start site
type: SequenceAnnotation
persistent identity: annotation1903922
display id: annotation1903922
version: 1
top level: BBa_R2111
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_R2111/annotation1903922/range1903922/1

https://synbiohub.org/public/igem/BBa_R2111/annotation1903922/range1903922/1
type: Range
persistent identity: range1903922
display id: range1903922
version: 1
direction: direction/forward
top level: BBa_R2111
start: 53
end: 53
orientation: inline

C2003 operator site
type: SequenceAnnotation
persistent identity: annotation1903923
display id: annotation1903923
version: 1
top level: BBa_R2111
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_R2111/annotation1903923/range1903923/1

https://synbiohub.org/public/igem/BBa_R2111/annotation1903923/range1903923/1
type: Range
persistent identity: range1903923
display id: range1903923
version: 1
direction: direction/forward
top level: BBa_R2111
start: 23
end: 34
orientation: inline

-10 site
type: SequenceAnnotation
persistent identity: annotation1903921
display id: annotation1903921
version: 1
top level: BBa_R2111
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R2111/annotation1903921/range1903921/1

https://synbiohub.org/public/igem/BBa_R2111/annotation1903921/range1903921/1
type: Range
persistent identity: range1903921
display id: range1903921
version: 1
direction: direction/forward
top level: BBa_R2111
start: 40
end: 45
orientation: inline

-35 site
type: SequenceAnnotation
persistent identity: annotation1903920
display id: annotation1903920
version: 1
top level: BBa_R2111
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R2111/annotation1903920/range1903920/1

https://synbiohub.org/public/igem/BBa_R2111/annotation1903920/range1903920/1
type: Range
persistent identity: range1903920
display id: range1903920
version: 1
direction: direction/forward
top level: BBa_R2111
start: 17
end: 22
orientation: inline

BBa_K823006
type: ComponentDefinition
persistent identity: BBa K823006
display id: BBa_K823006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Anderson promoter J23102
creator: Korinna Kraft
created: 2012-09-07T11:00:00Z
modified: 2015-07-07T11:45:05Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1081_
m user id: 4206
owner id: 12081
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Anderson promoter J23102 without RFP in backbone vector pSB1C3 to easily fuse the promoter with other reporters e.g. the lux operon.
mutable notes: no considerations
mutable provenance: Partsregistry
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -35 box
sequence annotation: -10 box
sequence annotation: J23102
sequence: https://synbiohub.org/public/igem/BBa_K823006_sequence/1

-35 box
type: SequenceAnnotation
persistent identity: annotation2182513
display id: annotation2182513
version: 1
top level: BBa_K823006
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K823006/annotation2182513/range2182513/1

https://synbiohub.org/public/igem/BBa_K823006/annotation2182513/range2182513/1
type: Range
persistent identity: range2182513
display id: range2182513
version: 1
direction: direction/forward
top level: BBa_K823006
start: 1
end: 6
orientation: inline

-10 box
type: SequenceAnnotation
persistent identity: annotation2182514
display id: annotation2182514
version: 1
top level: BBa_K823006
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K823006/annotation2182514/range2182514/1

https://synbiohub.org/public/igem/BBa_K823006/annotation2182514/range2182514/1
type: Range
persistent identity: range2182514
display id: range2182514
version: 1
direction: direction/forward
top level: BBa_K823006
start: 24
end: 29
orientation: inline

J23102
type: SequenceAnnotation
persistent identity: annotation2182512
display id: annotation2182512
version: 1
top level: BBa_K823006
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K823006/annotation2182512/range2182512/1

https://synbiohub.org/public/igem/BBa_K823006/annotation2182512/range2182512/1
type: Range
persistent identity: range2182512
display id: range2182512
version: 1
direction: direction/forward
top level: BBa_K823006
start: 1
end: 35
orientation: inline

BBa_K137049
type: ComponentDefinition
persistent identity: BBa K137049
display id: BBa_K137049
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: 450 bp inverted tetR promoter
creator: Allen Lin
created: 2008-07-20T11:00:00Z
modified: 2015-05-08T01:10:09Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _187_
m user id: 0
owner id: 3112
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Inverted tetR promoter with noncoding, spacer DNA upstream of it to make the total length 450 bp.
mutable notes: This part is in a series of inverted tetR promoters that have total lengths of 150, 250, 350, 450, 650, and 850 bp. We chose the region upstream of tetR in part Q04400 to be the noncoding DNA segment. This noncoding segment consists of the 3' end of tetR and B0015. None of the parts in this series was long enough to include the start codon of tetR, so a functional tetR protein should not be transcribed.
mutable provenance: Primers were synthesized to bind to part Q04400, and then a PCR reaction was run.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: tetR promotoer
sequence: https://synbiohub.org/public/igem/BBa_K137049_sequence/1

tetR promotoer
type: SequenceAnnotation
persistent identity: annotation1968022
display id: annotation1968022
version: 1
top level: BBa_K137049
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K137049/annotation1968022/range1968022/1

https://synbiohub.org/public/igem/BBa_K137049/annotation1968022/range1968022/1
type: Range
persistent identity: range1968022
display id: range1968022
version: 1
direction: direction/reverse
top level: BBa_K137049
start: 1
end: 54
orientation: inline

BBa_I739105
type: ComponentDefinition
persistent identity: BBa I739105
display id: BBa_I739105
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Double Promoter (LuxR/HSL, positive / cI, negative)
creator: Stefan Luzi
created: 2007-10-14T11:00:00Z
modified: 2015-08-31T04:08:01Z
discontinued: false
dominant: false
experience: experience/None
group u list: _124_
m user id: 0
owner id: 2183
owning group id: 62
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: asd
mutable notes: asdf
mutable provenance: Synthetic DNA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: R0051 (2x OR2)
sequence annotation: OR2
sequence annotation: R0062
sequence annotation: Scar
sequence: https://synbiohub.org/public/igem/BBa_I739105_sequence/1

R0051 (2x OR2)
type: SequenceAnnotation
persistent identity: annotation1947918
display id: annotation1947918
version: 1
top level: BBa_I739105
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_I739105/annotation1947918/range1947918/1

https://synbiohub.org/public/igem/BBa_I739105/annotation1947918/range1947918/1
type: Range
persistent identity: range1947918
display id: range1947918
version: 1
direction: direction/forward
top level: BBa_I739105
start: 64
end: 99
orientation: inline

OR2
type: SequenceAnnotation
persistent identity: annotation1947916
display id: annotation1947916
version: 1
top level: BBa_I739105
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I739105/annotation1947916/range1947916/1

https://synbiohub.org/public/igem/BBa_I739105/annotation1947916/range1947916/1
type: Range
persistent identity: range1947916
display id: range1947916
version: 1
direction: direction/forward
top level: BBa_I739105
start: 64
end: 81
orientation: inline

R0062
type: SequenceAnnotation
persistent identity: annotation1947914
display id: annotation1947914
version: 1
top level: BBa_I739105
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I739105/annotation1947914/range1947914/1

https://synbiohub.org/public/igem/BBa_I739105/annotation1947914/range1947914/1
type: Range
persistent identity: range1947914
display id: range1947914
version: 1
direction: direction/forward
top level: BBa_I739105
start: 1
end: 55
orientation: inline

OR2
type: SequenceAnnotation
persistent identity: annotation1947917
display id: annotation1947917
version: 1
top level: BBa_I739105
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I739105/annotation1947917/range1947917/1

https://synbiohub.org/public/igem/BBa_I739105/annotation1947917/range1947917/1
type: Range
persistent identity: range1947917
display id: range1947917
version: 1
direction: direction/forward
top level: BBa_I739105
start: 82
end: 99
orientation: inline

Scar
type: SequenceAnnotation
persistent identity: annotation1947915
display id: annotation1947915
version: 1
top level: BBa_I739105
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I739105/annotation1947915/range1947915/1

https://synbiohub.org/public/igem/BBa_I739105/annotation1947915/range1947915/1
type: Range
persistent identity: range1947915
display id: range1947915
version: 1
direction: direction/forward
top level: BBa_I739105
start: 56
end: 63
orientation: inline

BBa_J15301
type: ComponentDefinition
persistent identity: BBa J15301
display id: BBa_J15301
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Pars promoter from Escherichia coli chromosomal ars operon.
creator: Chris French
created: 2007-07-12T11:00:00Z
modified: 2015-08-31T04:08:32Z
discontinued: false
dominant: false
group u list: _163_
m user id: 0
owner id: 837
owning group id: 163
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is the promoter region of the Escherichia coli chromosomal ars promoter. It is repressed by ArsR in the absence of arsenate or arsenite (Cai, J., and DuBow, M.S. 1996. Expression of the Escherichia coli chromosomal ars operon. Canadian Journal of Microbiology 42, 662-671; Diorio, C., Cai, J., Marmor, J., Shinder, R., and DuBow, M.S. 1995. An Escherichia coli chromosomal ars operon homolog is functional in arsenic detoxification and is conserved in Gram negative bacteria. Journal of Bacteriology 177, 2050-2056).
mutable notes: No modifications were made to the sequence. There appears to be an unexpected repeat of ACT immediately before the SpeI site. This does not affect the biobrick suffix but means that the suffix is 5 bases downstream of the annotated transcriptional start site rather than 2 as recommended. The reason for this is unclear.
mutable provenance: Escherichia coli K12. Sequence obtained from X80057 (gi:510824). Regulatory sequence annotation from Cai, J., and DuBow, M.S. 1996. Expression of the Escherichia coli chromosomal ars operon. Canadian Journal of Microbiology 42, 662-671; Diorio, C., Cai, J., Marmor, J., Shinder, R., and DuBow, M.S. 1995. An Escherichia coli chromosomal ars operon homolog is functional in arsenic detoxification and is conserved in Gram negative bacteria. Journal of Bacteriology 177, 2050-2056.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -35
sequence annotation: ArsR binding
sequence annotation: -10
sequence annotation: +1
sequence: https://synbiohub.org/public/igem/BBa_J15301_sequence/1

-35
type: SequenceAnnotation
persistent identity: annotation1938076
display id: annotation1938076
version: 1
top level: BBa_J15301
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J15301/annotation1938076/range1938076/1

https://synbiohub.org/public/igem/BBa_J15301/annotation1938076/range1938076/1
type: Range
persistent identity: range1938076
display id: range1938076
version: 1
direction: direction/forward
top level: BBa_J15301
start: 88
end: 93
orientation: inline

ArsR binding
type: SequenceAnnotation
persistent identity: annotation1938075
display id: annotation1938075
version: 1
top level: BBa_J15301
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_J15301/annotation1938075/range1938075/1

https://synbiohub.org/public/igem/BBa_J15301/annotation1938075/range1938075/1
type: Range
persistent identity: range1938075
display id: range1938075
version: 1
direction: direction/forward
top level: BBa_J15301
start: 81
end: 85
orientation: inline

ArsR binding
type: SequenceAnnotation
persistent identity: annotation1938074
display id: annotation1938074
version: 1
top level: BBa_J15301
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_J15301/annotation1938074/range1938074/1

https://synbiohub.org/public/igem/BBa_J15301/annotation1938074/range1938074/1
type: Range
persistent identity: range1938074
display id: range1938074
version: 1
direction: direction/forward
top level: BBa_J15301
start: 67
end: 71
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1938077
display id: annotation1938077
version: 1
top level: BBa_J15301
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J15301/annotation1938077/range1938077/1

https://synbiohub.org/public/igem/BBa_J15301/annotation1938077/range1938077/1
type: Range
persistent identity: range1938077
display id: range1938077
version: 1
direction: direction/forward
top level: BBa_J15301
start: 111
end: 116
orientation: inline

+1
type: SequenceAnnotation
persistent identity: annotation1938078
display id: annotation1938078
version: 1
top level: BBa_J15301
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J15301/annotation1938078/range1938078/1

https://synbiohub.org/public/igem/BBa_J15301/annotation1938078/range1938078/1
type: Range
persistent identity: range1938078
display id: range1938078
version: 1
direction: direction/forward
top level: BBa_J15301
start: 123
end: 123
orientation: inline

BBa_K1585103
type: ComponentDefinition
persistent identity: BBa K1585103
display id: BBa_K1585103
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Anderson Promoter with lacI binding site
creator: Tobias Schwanemann
created: 2015-08-31T11:00:00Z
modified: 2015-09-01T03:08:18Z
discontinued: false
dominant: false
experience: experience/Fails
group u list: _2002_
m user id: 24474
owner id: 24474
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Promoters of the Anderson Promoter Collection with lacI binding site derived from [http://parts.igem.org/Part:BBa_R0010 R_0010] to make them [http://openwetware.org/wiki/IPTG IPTG] iducible.
mutable notes: We choosed lacI-binding-site of part BBa_R0010 because we assumed older parts would be more reliable. The part is shipped in backbone [http://parts.igem.org/Part:BBa_J61002 J61002] because the functionality is directly visible by adding IPTG. RFP ([http://parts.igem.org/Part:BBa_E1010 E1010]) should get expressed then.
mutable provenance: Assambly of [http://parts.igem.org/Part:BBa_J23103 J23103] and lacI-binding-site of [http://parts.igem.org/Part:BBa_R0010 R_0010] The "lacI binding site" of Part [http://parts.igem.org/Part:BBa_R0010 R_0010] differs from lacO from Part [http://parts.igem.org/Part:BBa_J33207 BBa_J33207].
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
component: BBa_J23103
component: BBa_K1585999
sequence annotation: BBa_K1585999
sequence annotation: BBa_J23103
sequence: https://synbiohub.org/public/igem/BBa_K1585103_sequence/1

BBa_J23103
type: Component
persistent identity: component2441411
display id: component2441411
version: 1
top level: BBa_K1585103
access: public
definition: BBa_J23103

BBa_K1585999
type: Component
persistent identity: component2441413
display id: component2441413
version: 1
top level: BBa_K1585103
access: public
definition: https://synbiohub.org/public/igem/BBa_K1585999/1

BBa_K1585999
type: SequenceAnnotation
persistent identity: annotation2441413
display id: annotation2441413
version: 1
top level: BBa_K1585103
location: https://synbiohub.org/public/igem/BBa_K1585103/annotation2441413/range2441413/1
component: BBa_K1585999

https://synbiohub.org/public/igem/BBa_K1585103/annotation2441413/range2441413/1
type: Range
persistent identity: range2441413
display id: range2441413
version: 1
direction: direction/reverse
top level: BBa_K1585103
start: 44
end: 78
orientation: inline

BBa_J23103
type: SequenceAnnotation
persistent identity: annotation2441411
display id: annotation2441411
version: 1
top level: BBa_K1585103
location: https://synbiohub.org/public/igem/BBa_K1585103/annotation2441411/range2441411/1
component: BBa_J23103

https://synbiohub.org/public/igem/BBa_K1585103/annotation2441411/range2441411/1
type: Range
persistent identity: range2441411
display id: range2441411
version: 1
direction: direction/reverse
top level: BBa_K1585103
start: 1
end: 35
orientation: inline

Pnot(O16)
type: ComponentDefinition
persistent identity: BBa I732271
display id: BBa_I732271
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member with Hybrid Operator (D001O16)
creator: Ma Rui
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:57Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732271_sequence/1

BBa_K1585118
type: ComponentDefinition
persistent identity: BBa K1585118
display id: BBa_K1585118
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Anderson Promoter with lacI binding site
creator: Tobias Schwanemann
created: 2015-08-31T11:00:00Z
modified: 2015-09-01T02:31:42Z
discontinued: false
dominant: false
experience: experience/Fails
group u list: _2002_
m user id: 24474
owner id: 24474
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Promoters of the Anderson Promoter Collection with lacI binding site derived from [http://parts.igem.org/Part:BBa_R0010 R_0010] to make them [http://openwetware.org/wiki/IPTG IPTG] iducible.
mutable notes: We choosed lacI-binding-site of part BBa_R0010 because we assumed older parts would be more reliable. The part is shipped in backbone [http://parts.igem.org/Part:BBa_J61002 J61002] because the functionality is directly visible by adding IPTG. RFP ([http://parts.igem.org/Part:BBa_E1010 E1010]) should get expressed then.
mutable provenance: Assambly of [http://parts.igem.org/Part:BBa_J23118 J23118] and lacI-binding-site of [http://parts.igem.org/Part:BBa_R0010 R_0010] The "lacI binding site" of Part [http://parts.igem.org/Part:BBa_R0010 R_0010] differs from lacO from Part [http://parts.igem.org/Part:BBa_J33207 BBa_J33207].
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
component: BBa_J23118
component: BBa_K1585999
sequence annotation: BBa_K1585999
sequence annotation: BBa_J23118
sequence: https://synbiohub.org/public/igem/BBa_K1585118_sequence/1

BBa_J23118
type: Component
persistent identity: component2441715
display id: component2441715
version: 1
top level: BBa_K1585118
access: public
definition: BBa_J23118

BBa_K1585999
type: Component
persistent identity: component2441717
display id: component2441717
version: 1
top level: BBa_K1585118
access: public
definition: https://synbiohub.org/public/igem/BBa_K1585999/1

BBa_K1585999
type: SequenceAnnotation
persistent identity: annotation2441717
display id: annotation2441717
version: 1
top level: BBa_K1585118
location: https://synbiohub.org/public/igem/BBa_K1585118/annotation2441717/range2441717/1
component: BBa_K1585999

https://synbiohub.org/public/igem/BBa_K1585118/annotation2441717/range2441717/1
type: Range
persistent identity: range2441717
display id: range2441717
version: 1
direction: direction/reverse
top level: BBa_K1585118
start: 44
end: 78
orientation: inline

BBa_J23118
type: SequenceAnnotation
persistent identity: annotation2441715
display id: annotation2441715
version: 1
top level: BBa_K1585118
location: https://synbiohub.org/public/igem/BBa_K1585118/annotation2441715/range2441715/1
component: BBa_J23118

https://synbiohub.org/public/igem/BBa_K1585118/annotation2441715/range2441715/1
type: Range
persistent identity: range2441715
display id: range2441715
version: 1
direction: direction/reverse
top level: BBa_K1585118
start: 1
end: 35
orientation: inline

BBa_M31370
type: ComponentDefinition
persistent identity: BBa M31370
display id: BBa_M31370
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: tacI Promoter
creator: Michael Oh
created: 2007-02-28T12:00:00Z
modified: 2015-05-08T01:14:00Z
discontinued: false
dominant: false
group u list: _102_
m user id: 0
owner id: 1395
owning group id: 102
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Allows for regulation of following gene with laqIq and IPTG...laqIq in same plasmid represses the promoter and IPTG proportionally depresses the promoter (Boer, et al., 1983).
mutable notes: Took sequence from Boer, 1983 paper.
mutable provenance: Derived from trp and lac promoter sequences.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_M31370_sequence/1

BBa_K1824895
type: ComponentDefinition
persistent identity: BBa K1824895
display id: BBa_K1824895
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ptet + RBS
creator: Zixu Wang
created: 2015-09-10T11:00:00Z
modified: 2015-09-11T09:46:05Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2250_
m user id: 26000
owner id: 26000
owning group id: 9
status: status/Planning
bookmark: false
mutable description: A inducible promoter plus a strong RBS B0034, repressed by TetR, induced by tetracycline and aTc.
mutable notes: This part should be used along with TetR.
mutable provenance: B0034 is based on Elowitz repressilator.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0034
component: BBa_K1824889
component: BBa_K1824890
component: BBa_R0040
sequence annotation: BBa_B0034
sequence annotation: BBa_K1824889
sequence annotation: BBa_R0040
sequence annotation: BBa_K1824890
sequence: https://synbiohub.org/public/igem/BBa_K1824895_sequence/1

BBa_B0034
type: Component
persistent identity: component2452337
display id: component2452337
version: 1
top level: BBa_K1824895
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K1824889
type: Component
persistent identity: component2452335
display id: component2452335
version: 1
top level: BBa_K1824895
access: public
definition: https://synbiohub.org/public/igem/BBa_K1824889/1

BBa_K1824890
type: Component
persistent identity: component2452338
display id: component2452338
version: 1
top level: BBa_K1824895
access: public
definition: https://synbiohub.org/public/igem/BBa_K1824890/1

BBa_R0040
type: Component
persistent identity: component2452330
display id: component2452330
version: 1
top level: BBa_K1824895
access: public
definition: p(tetR)

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2452337
display id: annotation2452337
version: 1
top level: BBa_K1824895
location: https://synbiohub.org/public/igem/BBa_K1824895/annotation2452337/range2452337/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1824895/annotation2452337/range2452337/1
type: Range
persistent identity: range2452337
display id: range2452337
version: 1
direction: direction/reverse
top level: BBa_K1824895
start: 63
end: 74
orientation: inline

BBa_K1824889
type: SequenceAnnotation
persistent identity: annotation2452335
display id: annotation2452335
version: 1
top level: BBa_K1824895
location: https://synbiohub.org/public/igem/BBa_K1824895/annotation2452335/range2452335/1
component: BBa_K1824889

https://synbiohub.org/public/igem/BBa_K1824895/annotation2452335/range2452335/1
type: Range
persistent identity: range2452335
display id: range2452335
version: 1
direction: direction/reverse
top level: BBa_K1824895
start: 55
end: 62
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2452330
display id: annotation2452330
version: 1
top level: BBa_K1824895
location: https://synbiohub.org/public/igem/BBa_K1824895/annotation2452330/range2452330/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_K1824895/annotation2452330/range2452330/1
type: Range
persistent identity: range2452330
display id: range2452330
version: 1
direction: direction/forward
top level: BBa_K1824895
start: 1
end: 54
orientation: inline

BBa_K1824890
type: SequenceAnnotation
persistent identity: annotation2452338
display id: annotation2452338
version: 1
top level: BBa_K1824895
location: https://synbiohub.org/public/igem/BBa_K1824895/annotation2452338/range2452338/1
component: BBa_K1824890

https://synbiohub.org/public/igem/BBa_K1824895/annotation2452338/range2452338/1
type: Range
persistent identity: range2452338
display id: range2452338
version: 1
direction: direction/reverse
top level: BBa_K1824895
start: 75
end: 80
orientation: inline

NOR2
type: ComponentDefinition
persistent identity: BBa I732352
display id: BBa_I732352
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: NOR Candidate (U035O44D001O22)
creator: Zhan Jian
created: 2007-10-02T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _156_
m user id: 0
owner id: 1592
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: U97NUL D001O61
mutable notes: .
mutable provenance: .
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732352_sequence/1

BBa_K206001
type: ComponentDefinition
persistent identity: BBa K206001
display id: BBa_K206001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pBAD weak
creator: Amelia Hardjasa
created: 2009-10-16T11:00:00Z
modified: 2015-05-08T01:11:23Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _307_
m user id: 0
owner id: 4172
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: Weaker version of wild type pBAD ( I13453 ).
mutable notes: No special considerations
mutable provenance: Site-directed mutagenesis on I13453 with the following primers: Forward: TAATCTTATGGATAAAAAAGCTATGGCATAGC Reverse: GCGGATCCTACCTGACGCTTTTTATC
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: promoter
sequence annotation: AraI2
sequence annotation: AraI1
sequence: https://synbiohub.org/public/igem/BBa_K206001_sequence/1

promoter
type: SequenceAnnotation
persistent identity: annotation2049255
display id: annotation2049255
version: 1
top level: BBa_K206001
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K206001/annotation2049255/range2049255/1

https://synbiohub.org/public/igem/BBa_K206001/annotation2049255/range2049255/1
type: Range
persistent identity: range2049255
display id: range2049255
version: 1
direction: direction/forward
top level: BBa_K206001
start: 1
end: 131
orientation: inline

AraI2
type: SequenceAnnotation
persistent identity: annotation2049257
display id: annotation2049257
version: 1
top level: BBa_K206001
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K206001/annotation2049257/range2049257/1

https://synbiohub.org/public/igem/BBa_K206001/annotation2049257/range2049257/1
type: Range
persistent identity: range2049257
display id: range2049257
version: 1
direction: direction/forward
top level: BBa_K206001
start: 61
end: 78
orientation: inline

AraI1
type: SequenceAnnotation
persistent identity: annotation2049256
display id: annotation2049256
version: 1
top level: BBa_K206001
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K206001/annotation2049256/range2049256/1

https://synbiohub.org/public/igem/BBa_K206001/annotation2049256/range2049256/1
type: Range
persistent identity: range2049256
display id: range2049256
version: 1
direction: direction/forward
top level: BBa_K206001
start: 40
end: 57
orientation: inline

CreAp
type: ComponentDefinition
persistent identity: BBa J64951
display id: BBa_J64951
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: E. Coli CreABCD phosphate sensing operon promoter
creator: reid williams
created: 2007-03-19T12:00:00Z
modified: 2015-05-08T01:08:17Z
discontinued: false
dominant: false
experience: experience/None
group u list: _98_
m user id: 0
owner id: 1432
owning group id: 98
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: CreABCD phosphate sensing operon promoter taken from RegulonDB.
mutable notes: none
mutable provenance: E. Coli K12
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J64951_sequence/1

BBa_K1585105
type: ComponentDefinition
persistent identity: BBa K1585105
display id: BBa_K1585105
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Anderson Promoter with lacI binding site
creator: Tobias Schwanemann
created: 2015-08-31T11:00:00Z
modified: 2015-09-01T02:03:04Z
discontinued: false
dominant: false
experience: experience/Fails
group u list: _2002_
m user id: 24474
owner id: 24474
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Promoters of the Anderson Promoter Collection with lacI binding site derived from [http://parts.igem.org/Part:BBa_R0010 R_0010] to make them [http://openwetware.org/wiki/IPTG IPTG] iducible.
mutable notes: We choosed lacI-binding-site of part BBa_R0010 because we assumed older parts would be more reliable. The part is shipped in backbone [http://parts.igem.org/Part:BBa_J61002 J61002] because the functionality is directly visible by adding IPTG. RFP ([http://parts.igem.org/Part:BBa_E1010 E1010]) should get expressed then.
mutable provenance: Assambly of [http://parts.igem.org/Part:BBa_J23103 J23103] and lacI-binding-site of [http://parts.igem.org/Part:BBa_R0010 R_0010] The "lacI binding site" of Part [http://parts.igem.org/Part:BBa_R0010 R_0010] differs from lacO from Part [http://parts.igem.org/Part:BBa_J33207 BBa_J33207].
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
component: BBa_K1585999
component: BBa_J23105
sequence annotation: BBa_J23105
sequence annotation: BBa_K1585999
sequence: https://synbiohub.org/public/igem/BBa_K1585105_sequence/1

BBa_K1585999
type: Component
persistent identity: component2441416
display id: component2441416
version: 1
top level: BBa_K1585105
access: public
definition: https://synbiohub.org/public/igem/BBa_K1585999/1

BBa_J23105
type: Component
persistent identity: component2441414
display id: component2441414
version: 1
top level: BBa_K1585105
access: public
definition: BBa_J23105

BBa_J23105
type: SequenceAnnotation
persistent identity: annotation2441414
display id: annotation2441414
version: 1
top level: BBa_K1585105
location: https://synbiohub.org/public/igem/BBa_K1585105/annotation2441414/range2441414/1
component: BBa_J23105

https://synbiohub.org/public/igem/BBa_K1585105/annotation2441414/range2441414/1
type: Range
persistent identity: range2441414
display id: range2441414
version: 1
direction: direction/reverse
top level: BBa_K1585105
start: 1
end: 35
orientation: inline

BBa_K1585999
type: SequenceAnnotation
persistent identity: annotation2441416
display id: annotation2441416
version: 1
top level: BBa_K1585105
location: https://synbiohub.org/public/igem/BBa_K1585105/annotation2441416/range2441416/1
component: BBa_K1585999

https://synbiohub.org/public/igem/BBa_K1585105/annotation2441416/range2441416/1
type: Range
persistent identity: range2441416
display id: range2441416
version: 1
direction: direction/reverse
top level: BBa_K1585105
start: 44
end: 78
orientation: inline

U037O11+D0
type: ComponentDefinition
persistent identity: BBa I732443
display id: BBa_I732443
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member (U037O11+D002O22)
creator: Zhan Jian
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732443_sequence/1

U037O11+D0
type: ComponentDefinition
persistent identity: BBa I732445
display id: BBa_I732445
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member (U037O11+D026O22)
creator: Zhan Jian
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732445_sequence/1

BBa_J44002
type: ComponentDefinition
persistent identity: BBa J44002
display id: BBa_J44002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pBAD reverse
creator: Brad Ogden
created: 2006-08-15T11:00:00Z
modified: 2015-08-31T04:08:48Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _71_
m user id: 0
owner id: 811
owning group id: 71
sample status: In stock
status: status/Available
bookmark: true
mutable description: This is the pBAD promoter (BBa_I13453) in the opposite orientation. It can be used to drive transcription in the direction of suffix to prefix.
mutable notes: None.
mutable provenance: Cloned from synthetic oligonucleotides.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: promoter
sequence: https://synbiohub.org/public/igem/BBa_J44002_sequence/1

promoter
type: SequenceAnnotation
persistent identity: annotation2002776
display id: annotation2002776
version: 1
top level: BBa_J44002
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J44002/annotation2002776/range2002776/1

https://synbiohub.org/public/igem/BBa_J44002/annotation2002776/range2002776/1
type: Range
persistent identity: range2002776
display id: range2002776
version: 1
direction: direction/reverse
top level: BBa_J44002
start: 1
end: 130
orientation: inline

BBa_K180012
type: ComponentDefinition
persistent identity: BBa K180012
display id: BBa_K180012
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Rock - Primary plasmid (part 2) [tac pR, mCherry and LasI generator]
creator: Richard Wilson, Stephanie Chambers
created: 2009-10-18T11:00:00Z
modified: 2015-05-08T01:11:06Z
discontinued: false
dominant: false
experience: experience/None
group u list: _278_
m user id: 0
owner id: 4082
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Part of the iGEM2009 Team:Southampton rock-paper-scissors 'rock' primary plasmid.
mutable notes: Regulated expression of LasI (C0178) and mCherry (K180008) by tac pR (K180000), controlled by presence of lac/IPTG or trp in the cell.
mutable provenance: See relevant basic biobrick parts (K180000, B0034, K180008, B0015)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0012
component: BBa_B0034
component: BBa_C0178
component: BBa_K180000
component: BBa_B0010
component: BBa_K180008
sequence annotation: BBa_B0034
sequence annotation: BBa_K180000
sequence annotation: BBa_B0010
sequence annotation: BBa_C0178
sequence annotation: BBa_K180008
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_K180012_sequence/1

BBa_B0012
type: Component
persistent identity: component2053935
display id: component2053935
version: 1
top level: BBa_K180012
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0034
type: Component
persistent identity: component2053930
display id: component2053930
version: 1
top level: BBa_K180012
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_C0178
type: Component
persistent identity: component2053932
display id: component2053932
version: 1
top level: BBa_K180012
access: public
definition: https://synbiohub.org/public/igem/BBa_C0178/1

BBa_B0034
type: Component
persistent identity: component2053926
display id: component2053926
version: 1
top level: BBa_K180012
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K180000
type: Component
persistent identity: component2053924
display id: component2053924
version: 1
top level: BBa_K180012
access: public
definition: BBa_K180000

BBa_B0010
type: Component
persistent identity: component2053933
display id: component2053933
version: 1
top level: BBa_K180012
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_K180008
type: Component
persistent identity: component2053928
display id: component2053928
version: 1
top level: BBa_K180012
access: public
definition: https://synbiohub.org/public/igem/BBa_K180008/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2053926
display id: annotation2053926
version: 1
top level: BBa_K180012
location: https://synbiohub.org/public/igem/BBa_K180012/annotation2053926/range2053926/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K180012/annotation2053926/range2053926/1
type: Range
persistent identity: range2053926
display id: range2053926
version: 1
direction: direction/reverse
top level: BBa_K180012
start: 1456
end: 1467
orientation: inline

BBa_K180000
type: SequenceAnnotation
persistent identity: annotation2053924
display id: annotation2053924
version: 1
top level: BBa_K180012
location: https://synbiohub.org/public/igem/BBa_K180012/annotation2053924/range2053924/1
component: BBa_K180000

https://synbiohub.org/public/igem/BBa_K180012/annotation2053924/range2053924/1
type: Range
persistent identity: range2053924
display id: range2053924
version: 1
direction: direction/reverse
top level: BBa_K180012
start: 1
end: 1447
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2053933
display id: annotation2053933
version: 1
top level: BBa_K180012
location: https://synbiohub.org/public/igem/BBa_K180012/annotation2053933/range2053933/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K180012/annotation2053933/range2053933/1
type: Range
persistent identity: range2053933
display id: range2053933
version: 1
direction: direction/forward
top level: BBa_K180012
start: 2825
end: 2904
orientation: inline

BBa_C0178
type: SequenceAnnotation
persistent identity: annotation2053932
display id: annotation2053932
version: 1
top level: BBa_K180012
location: https://synbiohub.org/public/igem/BBa_K180012/annotation2053932/range2053932/1
component: BBa_C0178

https://synbiohub.org/public/igem/BBa_K180012/annotation2053932/range2053932/1
type: Range
persistent identity: range2053932
display id: range2053932
version: 1
direction: direction/reverse
top level: BBa_K180012
start: 2208
end: 2816
orientation: inline

BBa_K180008
type: SequenceAnnotation
persistent identity: annotation2053928
display id: annotation2053928
version: 1
top level: BBa_K180012
location: https://synbiohub.org/public/igem/BBa_K180012/annotation2053928/range2053928/1
component: BBa_K180008

https://synbiohub.org/public/igem/BBa_K180012/annotation2053928/range2053928/1
type: Range
persistent identity: range2053928
display id: range2053928
version: 1
direction: direction/reverse
top level: BBa_K180012
start: 1474
end: 2181
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2053930
display id: annotation2053930
version: 1
top level: BBa_K180012
location: https://synbiohub.org/public/igem/BBa_K180012/annotation2053930/range2053930/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K180012/annotation2053930/range2053930/1
type: Range
persistent identity: range2053930
display id: range2053930
version: 1
direction: direction/reverse
top level: BBa_K180012
start: 2190
end: 2201
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2053935
display id: annotation2053935
version: 1
top level: BBa_K180012
location: https://synbiohub.org/public/igem/BBa_K180012/annotation2053935/range2053935/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K180012/annotation2053935/range2053935/1
type: Range
persistent identity: range2053935
display id: range2053935
version: 1
direction: direction/forward
top level: BBa_K180012
start: 2913
end: 2953
orientation: inline

const.
type: ComponentDefinition
persistent identity: BBa R1075
display id: BBa_R1075
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Constitutive Promoter II
creator: Chris Walsh (Fighting Darwins)
created: 2004-01-27T12:00:00Z
modified: 2015-05-08T01:14:15Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: constitutive promoter which outputs .4 TIPs point mutants of wild type rrnB promoter P1
mutable provenance: Gaal, Gourse, et al.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -35
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R1075_sequence/1

-35
type: SequenceAnnotation
persistent identity: annotation309024
display id: annotation309024
version: 1
top level: const.
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R1075/annotation309024/range309024/1

https://synbiohub.org/public/igem/BBa_R1075/annotation309024/range309024/1
type: Range
persistent identity: range309024
display id: range309024
version: 1
direction: direction/forward
top level: const.
start: 15
end: 20
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation309020
display id: annotation309020
version: 1
top level: const.
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R1075/annotation309020/range309020/1

https://synbiohub.org/public/igem/BBa_R1075/annotation309020/range309020/1
type: Range
persistent identity: range309020
display id: range309020
version: 1
direction: direction/forward
top level: const.
start: 35
end: 40
orientation: inline

Pnot(O44)
type: ComponentDefinition
persistent identity: BBa I732204
display id: BBa_I732204
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: NOT Gate Promoter Family Member (D001O44)
creator: Zhan Jian
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:57Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732204_sequence/1

BBa_J23106
type: ComponentDefinition
persistent identity: BBa J23106
display id: BBa_J23106
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: constitutive promoter family member
creator: John Anderson
created: 2006-08-13T11:00:00Z
modified: 2015-08-31T04:08:40Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: Later
mutable notes: N/A
mutable provenance: Isolated from library of promoters
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J23106_sequence/1

NAND01
type: ComponentDefinition
persistent identity: BBa I732303
display id: BBa_I732303
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: NAND Candidate (U073O22D001O46)
creator: Ma Rui
created: 2007-10-02T11:00:00Z
modified: 2015-08-31T04:07:57Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _156_
m user id: 0
owner id: 1592
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: U97NUL D001O11
mutable notes: .
mutable provenance: .
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732303_sequence/1

BBa_J05210
type: ComponentDefinition
persistent identity: BBa J05210
display id: BBa_J05210
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Modifed Prm+ Promoter
creator: Alexander Roth
created: 2005-09-05T11:00:00Z
modified: 2015-08-31T04:08:15Z
discontinued: false
dominant: false
group u list: _34_
m user id: 0
owner id: 412
owning group id: 34
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This is identical to BBa_I12007 exept that some base has been modifed to allow for additional ZFP repressors to bind.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: OR2
sequence annotation: -10
sequence annotation: -35
sequence annotation: ZFP binding sites
sequence annotation: OR1
sequence: https://synbiohub.org/public/igem/BBa_J05210_sequence/1

OR2
type: SequenceAnnotation
persistent identity: annotation1689958
display id: annotation1689958
version: 1
top level: BBa_J05210
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_J05210/annotation1689958/range1689958/1

https://synbiohub.org/public/igem/BBa_J05210/annotation1689958/range1689958/1
type: Range
persistent identity: range1689958
display id: range1689958
version: 1
direction: direction/forward
top level: BBa_J05210
start: 33
end: 49
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1689961
display id: annotation1689961
version: 1
top level: BBa_J05210
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J05210/annotation1689961/range1689961/1

https://synbiohub.org/public/igem/BBa_J05210/annotation1689961/range1689961/1
type: Range
persistent identity: range1689961
display id: range1689961
version: 1
direction: direction/forward
top level: BBa_J05210
start: 71
end: 76
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1689959
display id: annotation1689959
version: 1
top level: BBa_J05210
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J05210/annotation1689959/range1689959/1

https://synbiohub.org/public/igem/BBa_J05210/annotation1689959/range1689959/1
type: Range
persistent identity: range1689959
display id: range1689959
version: 1
direction: direction/forward
top level: BBa_J05210
start: 48
end: 53
orientation: inline

ZFP binding sites
type: SequenceAnnotation
persistent identity: annotation1689960
display id: annotation1689960
version: 1
top level: BBa_J05210
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_J05210/annotation1689960/range1689960/1

https://synbiohub.org/public/igem/BBa_J05210/annotation1689960/range1689960/1
type: Range
persistent identity: range1689960
display id: range1689960
version: 1
direction: direction/forward
top level: BBa_J05210
start: 55
end: 66
orientation: inline

OR1
type: SequenceAnnotation
persistent identity: annotation1689957
display id: annotation1689957
version: 1
top level: BBa_J05210
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_J05210/annotation1689957/range1689957/1

https://synbiohub.org/public/igem/BBa_J05210/annotation1689957/range1689957/1
type: Range
persistent identity: range1689957
display id: range1689957
version: 1
direction: direction/forward
top level: BBa_J05210
start: 9
end: 25
orientation: inline

BBa_K1682003
type: ComponentDefinition
persistent identity: BBa K1682003
display id: BBa_K1682003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PkdpF[-15, T>C]
creator: iGEM15_HKUST-Rice
created: 2015-08-09T11:00:00Z
modified: 2015-09-18T09:16:08Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2100_
m user id: 8758
owner id: 21235
owning group id: 9
status: status/Planning
bookmark: false
mutable description: kdpFp[-15, T>C]
mutable notes: kdpFp[-15, T>C]
mutable provenance: kdpFp[-15, T>C]
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: EcoRI Illegal site removed T->C
sequence annotation: -35
sequence annotation: phospho-KdpE binding site
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_K1682003_sequence/1

EcoRI Illegal site removed T->C
type: SequenceAnnotation
persistent identity: annotation2475214
display id: annotation2475214
version: 1
top level: BBa_K1682003
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K1682003/annotation2475214/range2475214/1

https://synbiohub.org/public/igem/BBa_K1682003/annotation2475214/range2475214/1
type: Range
persistent identity: range2475214
display id: range2475214
version: 1
direction: direction/forward
top level: BBa_K1682003
start: 65
end: 66
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2473590
display id: annotation2473590
version: 1
top level: BBa_K1682003
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1682003/annotation2473590/range2473590/1

https://synbiohub.org/public/igem/BBa_K1682003/annotation2473590/range2473590/1
type: Range
persistent identity: range2473590
display id: range2473590
version: 1
direction: direction/forward
top level: BBa_K1682003
start: 42
end: 47
orientation: inline

phospho-KdpE binding site
type: SequenceAnnotation
persistent identity: annotation2473591
display id: annotation2473591
version: 1
top level: BBa_K1682003
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1682003/annotation2473591/range2473591/1

https://synbiohub.org/public/igem/BBa_K1682003/annotation2473591/range2473591/1
type: Range
persistent identity: range2473591
display id: range2473591
version: 1
direction: direction/forward
top level: BBa_K1682003
start: 8
end: 24
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2473589
display id: annotation2473589
version: 1
top level: BBa_K1682003
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1682003/annotation2473589/range2473589/1

https://synbiohub.org/public/igem/BBa_K1682003/annotation2473589/range2473589/1
type: Range
persistent identity: range2473589
display id: range2473589
version: 1
direction: direction/forward
top level: BBa_K1682003
start: 69
end: 74
orientation: inline

Pnot(O26)
type: ComponentDefinition
persistent identity: BBa I732275
display id: BBa_I732275
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member with Hybrid Operator (D001O26)
creator: Ma Rui
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:57Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732275_sequence/1

BBa_K292000
type: ComponentDefinition
persistent identity: BBa K292000
display id: BBa_K292000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Double terminator + constitutive promoter
creator: David Charoy
created: 2009-10-17T11:00:00Z
modified: 2015-05-08T01:11:49Z
discontinued: false
dominant: true
group u list: _394_
m user id: 0
owner id: 5212
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part contains a double terminator and a constitutive prokaryotic promoter. It is a simple promoter to begin gene transcription. For Sup???biotech-Paris (2009) team this part is used after to be added to a recombinant lambda phage to begin the transcription of synthetic genes inside the Lambda phage genome.
mutable notes: N/A
mutable provenance: BBa_B0014 : Designed by Reshma Shetty Group: Registry (2003-07-16) BBa_J23100 : Designed by John Anderson Group: iGEM2006_Berkeley (2006-08-04)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_J23100
component: BBa_B0012
component: BBa_B0011
sequence annotation: BBa_J23100
sequence annotation: BBa_B0011
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_K292000_sequence/1

BBa_J23100
type: Component
persistent identity: component2059163
display id: component2059163
version: 1
top level: BBa_K292000
access: public
definition: BBa_J23100

BBa_B0012
type: Component
persistent identity: component2059157
display id: component2059157
version: 1
top level: BBa_K292000
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0011
type: Component
persistent identity: component2059161
display id: component2059161
version: 1
top level: BBa_K292000
access: public
definition: https://synbiohub.org/public/igem/BBa_B0011/1

BBa_J23100
type: SequenceAnnotation
persistent identity: annotation2059163
display id: annotation2059163
version: 1
top level: BBa_K292000
location: https://synbiohub.org/public/igem/BBa_K292000/annotation2059163/range2059163/1
component: BBa_J23100

https://synbiohub.org/public/igem/BBa_K292000/annotation2059163/range2059163/1
type: Range
persistent identity: range2059163
display id: range2059163
version: 1
direction: direction/reverse
top level: BBa_K292000
start: 104
end: 138
orientation: inline

BBa_B0011
type: SequenceAnnotation
persistent identity: annotation2059161
display id: annotation2059161
version: 1
top level: BBa_K292000
location: https://synbiohub.org/public/igem/BBa_K292000/annotation2059161/range2059161/1
component: BBa_B0011

https://synbiohub.org/public/igem/BBa_K292000/annotation2059161/range2059161/1
type: Range
persistent identity: range2059161
display id: range2059161
version: 1
direction: direction/forward
top level: BBa_K292000
start: 50
end: 95
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2059157
display id: annotation2059157
version: 1
top level: BBa_K292000
location: https://synbiohub.org/public/igem/BBa_K292000/annotation2059157/range2059157/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K292000/annotation2059157/range2059157/1
type: Range
persistent identity: range2059157
display id: range2059157
version: 1
direction: direction/forward
top level: BBa_K292000
start: 1
end: 41
orientation: inline

BBa_J22106
type: ComponentDefinition
persistent identity: BBa J22106
display id: BBa_J22106
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: rec A (SOS) Promoter
creator: Krithiga Sankaran
created: 2006-09-04T11:00:00Z
modified: 2015-08-31T04:08:38Z
discontinued: false
dominant: true
group u list: _70_
m user id: 0
owner id: 1038
owning group id: 70
sample status: In stock
status: status/Available
bookmark: false
mutable description: Rec A (SOS) promoter.
mutable notes: NA
mutable provenance: The recA(SOS)promoter gene from the chromosomal DNA isolated from MG1655 has been PCR amplified and inserted into plasmid pSB1A2(part number BBa_B0015).
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J22106_sequence/1

Cat
type: ComponentDefinition
persistent identity: BBa I14033
display id: BBa_I14033
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: P(Cat)
creator: Vikram Vijayan, Allen Hsu, Lawrence Fomundam
created: 2004-08-03T11:00:00Z
modified: 2015-08-31T04:07:37Z
discontinued: false
dominant: false
experience: experience/None
group u list: _4_
m user id: 0
owner id: 171
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Constitutive Promoter, Medium Transcription
mutable provenance: Plasmid pACYC184
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -10
sequence annotation: -35
sequence annotation: P(Cat)
sequence: https://synbiohub.org/public/igem/BBa_I14033_sequence/1

-10
type: SequenceAnnotation
persistent identity: annotation1026173
display id: annotation1026173
version: 1
top level: Cat
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I14033/annotation1026173/range1026173/1

https://synbiohub.org/public/igem/BBa_I14033/annotation1026173/range1026173/1
type: Range
persistent identity: range1026173
display id: range1026173
version: 1
direction: direction/forward
top level: Cat
start: 27
end: 32
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1026172
display id: annotation1026172
version: 1
top level: Cat
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I14033/annotation1026172/range1026172/1

https://synbiohub.org/public/igem/BBa_I14033/annotation1026172/range1026172/1
type: Range
persistent identity: range1026172
display id: range1026172
version: 1
direction: direction/forward
top level: Cat
start: 4
end: 9
orientation: inline

P(Cat)
type: SequenceAnnotation
persistent identity: annotation1026174
display id: annotation1026174
version: 1
top level: Cat
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_I14033/annotation1026174/range1026174/1

https://synbiohub.org/public/igem/BBa_I14033/annotation1026174/range1026174/1
type: Range
persistent identity: range1026174
display id: range1026174
version: 1
direction: direction/forward
top level: Cat
start: 1
end: 38
orientation: inline

cI lam
type: ComponentDefinition
persistent identity: BBa R1051
display id: BBa_R1051
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter, Standard (lambda cI regulated)
creator: Vinay S Mahajan, Brian Chow, Peter Carr, Voichita Marinescu and Alexander D. Wissner-Gross Drew Endy
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:15Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: [Note: This is the same part as R0051 except that the -10 and -35 sites and spacing have been changed to comply with BBa_S0001]. The cI regulated promoter is based on the pR promtoer from bacteriohage lambda. The promoter has two two DNA binding sites for lambda cI repressor <bb_part>BBa_C0051</bb_part>. cI binding results in repression of transcription. The specific sequence used here is based on the cI repressible promoter used in the Elowitz repressilator (and references therein).
mutable notes: In order to address concerns about the promoter transcribing in the reverse direction, we have removed the -35 and -10 signals responsible for the promoter activity in the reverse direction. (More details needed here! DE, 2/24/03) Modified to comply with BBa_S0001: TTGACA-17N-GATACT Incompatible with host expressing cI repressor.
mutable provenance: A synthetic oscillatory network of transcriptional regulators , Elowitz M.B. , Leibler S., Nature(403),335-38: 2000
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: OR1
sequence annotation: -35
sequence annotation: -10
sequence annotation: BBa_R1051
sequence annotation: OR2
sequence: https://synbiohub.org/public/igem/BBa_R1051_sequence/1

OR1
type: SequenceAnnotation
persistent identity: annotation2076
display id: annotation2076
version: 1
top level: cI lam
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R1051/annotation2076/range2076/1

https://synbiohub.org/public/igem/BBa_R1051/annotation2076/range2076/1
type: Range
persistent identity: range2076
display id: range2076
version: 1
direction: direction/forward
top level: cI lam
start: 25
end: 41
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2075
display id: annotation2075
version: 1
top level: cI lam
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R1051/annotation2075/range2075/1

https://synbiohub.org/public/igem/BBa_R1051/annotation2075/range2075/1
type: Range
persistent identity: range2075
display id: range2075
version: 1
direction: direction/forward
top level: cI lam
start: 15
end: 20
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2078
display id: annotation2078
version: 1
top level: cI lam
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R1051/annotation2078/range2078/1

https://synbiohub.org/public/igem/BBa_R1051/annotation2078/range2078/1
type: Range
persistent identity: range2078
display id: range2078
version: 1
direction: direction/forward
top level: cI lam
start: 38
end: 43
orientation: inline

BBa_R1051
type: SequenceAnnotation
persistent identity: annotation7074
display id: annotation7074
version: 1
top level: cI lam
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_R1051/annotation7074/range7074/1

https://synbiohub.org/public/igem/BBa_R1051/annotation7074/range7074/1
type: Range
persistent identity: range7074
display id: range7074
version: 1
direction: direction/forward
top level: cI lam
start: 1
end: 49
orientation: inline

OR2
type: SequenceAnnotation
persistent identity: annotation2077
display id: annotation2077
version: 1
top level: cI lam
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R1051/annotation2077/range2077/1

https://synbiohub.org/public/igem/BBa_R1051/annotation2077/range2077/1
type: Range
persistent identity: range2077
display id: range2077
version: 1
direction: direction/forward
top level: cI lam
start: 1
end: 17
orientation: inline

BBa_I761011
type: ComponentDefinition
persistent identity: BBa I761011
display id: BBa_I761011
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CinR, CinL and glucose controlled promotor
creator: 2007 NYMU_Taiwan iGEM Team
created: 2007-10-18T11:00:00Z
modified: 2015-08-31T04:08:08Z
discontinued: false
dominant: false
experience: experience/None
group u list: _140_
m user id: 0
owner id: 2031
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This promotor is controlled by cinr, cinl and extrcellular glucose concentration. High extracellular glucose concentration will inhibit this promotor through the action of phosphorylated OmpR. Phosphorylated OmpR will bind the OmpR binding site of the promotor and block the movement of RNA polymerase. In contrast, the combined action of CinR and CinL will turn on the promotor. But only when the extracellular glucose level is low so OmpR dephosphorylate and dissociate from its binding site will this promotor become active. In essence, this promotor function as an AND gate, it will turn on if and only if the extracellular glucose level is low and there are some functional CinR and CinL protein inside the cell.
mutable notes: The key to the function of the promotor is the inhibitory ability of OmpR binding site. To ensure maximum affinity with phosphorylated OmpR, we use all three OmpR binding site.s.
mutable provenance: *BBa_R0078 *BBa_I761001
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
component: BBa_I761001
component: BBa_R0078
sequence annotation: BBa_R0078
sequence annotation: BBa_I761001
sequence: https://synbiohub.org/public/igem/BBa_I761011_sequence/1

BBa_I761001
type: Component
persistent identity: component1950122
display id: component1950122
version: 1
top level: BBa_I761011
access: public
definition: https://synbiohub.org/public/igem/BBa_I761001/1

BBa_R0078
type: Component
persistent identity: component1950118
display id: component1950118
version: 1
top level: BBa_I761011
access: public
definition: cinR

BBa_R0078
type: SequenceAnnotation
persistent identity: annotation1950118
display id: annotation1950118
version: 1
top level: BBa_I761011
location: https://synbiohub.org/public/igem/BBa_I761011/annotation1950118/range1950118/1
component: BBa_R0078

https://synbiohub.org/public/igem/BBa_I761011/annotation1950118/range1950118/1
type: Range
persistent identity: range1950118
display id: range1950118
version: 1
direction: direction/reverse
top level: BBa_I761011
start: 1
end: 225
orientation: inline

BBa_I761001
type: SequenceAnnotation
persistent identity: annotation1950122
display id: annotation1950122
version: 1
top level: BBa_I761011
location: https://synbiohub.org/public/igem/BBa_I761011/annotation1950122/range1950122/1
component: BBa_I761001

https://synbiohub.org/public/igem/BBa_I761011/annotation1950122/range1950122/1
type: Range
persistent identity: range1950122
display id: range1950122
version: 1
direction: direction/reverse
top level: BBa_I761011
start: 234
end: 295
orientation: inline

BBa_K1585106
type: ComponentDefinition
persistent identity: BBa K1585106
display id: BBa_K1585106
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Anderson Promoter with lacI binding site
creator: Tobias Schwanemann
created: 2015-08-31T11:00:00Z
modified: 2015-09-01T02:01:39Z
discontinued: false
dominant: false
experience: experience/Fails
group u list: _2002_
m user id: 24474
owner id: 24474
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Promoters of the Anderson Promoter Collection with lacI binding site derived from [http://parts.igem.org/Part:BBa_R0010 R_0010] to make them [http://openwetware.org/wiki/IPTG IPTG] iducible.
mutable notes: We choosed lacI-binding-site of part BBa_R0010 because we assumed older parts would be more reliable. The part is shipped in backbone [http://parts.igem.org/Part:BBa_J61002 J61002] because the functionality is directly visible by adding IPTG. RFP ([http://parts.igem.org/Part:BBa_E1010 E1010]) should get expressed then.
mutable provenance: Assambly of [http://parts.igem.org/Part:BBa_J23106 J23106] and lacI-binding-site of [http://parts.igem.org/Part:BBa_R0010 R_0010] The "lacI binding site" of Part [http://parts.igem.org/Part:BBa_R0010 R_0010] differs from lacO from Part [http://parts.igem.org/Part:BBa_J33207 BBa_J33207].
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
component: BBa_J23106
component: BBa_K1585999
sequence annotation: BBa_J23106
sequence annotation: BBa_K1585999
sequence: https://synbiohub.org/public/igem/BBa_K1585106_sequence/1

BBa_J23106
type: Component
persistent identity: component2441696
display id: component2441696
version: 1
top level: BBa_K1585106
access: public
definition: BBa_J23106

BBa_K1585999
type: Component
persistent identity: component2441698
display id: component2441698
version: 1
top level: BBa_K1585106
access: public
definition: https://synbiohub.org/public/igem/BBa_K1585999/1

BBa_J23106
type: SequenceAnnotation
persistent identity: annotation2441696
display id: annotation2441696
version: 1
top level: BBa_K1585106
location: https://synbiohub.org/public/igem/BBa_K1585106/annotation2441696/range2441696/1
component: BBa_J23106

https://synbiohub.org/public/igem/BBa_K1585106/annotation2441696/range2441696/1
type: Range
persistent identity: range2441696
display id: range2441696
version: 1
direction: direction/reverse
top level: BBa_K1585106
start: 1
end: 35
orientation: inline

BBa_K1585999
type: SequenceAnnotation
persistent identity: annotation2441698
display id: annotation2441698
version: 1
top level: BBa_K1585106
location: https://synbiohub.org/public/igem/BBa_K1585106/annotation2441698/range2441698/1
component: BBa_K1585999

https://synbiohub.org/public/igem/BBa_K1585106/annotation2441698/range2441698/1
type: Range
persistent identity: range2441698
display id: range2441698
version: 1
direction: direction/reverse
top level: BBa_K1585106
start: 44
end: 78
orientation: inline

BBa_I739102
type: ComponentDefinition
persistent identity: BBa I739102
display id: BBa_I739102
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Double Promoter (cI, negative / TetR, negative)
creator: Stefan Luzi
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:08:01Z
discontinued: false
dominant: false
group u list: _124_
m user id: 0
owner id: 2183
owning group id: 62
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: asdf
mutable notes: asdf
mutable provenance: Synthetic DNA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: Scar
sequence annotation: TetR 1
sequence annotation: R0040 (2x TetR 1)
sequence annotation: R0051
sequence: https://synbiohub.org/public/igem/BBa_I739102_sequence/1

Scar
type: SequenceAnnotation
persistent identity: annotation1946931
display id: annotation1946931
version: 1
top level: BBa_I739102
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I739102/annotation1946931/range1946931/1

https://synbiohub.org/public/igem/BBa_I739102/annotation1946931/range1946931/1
type: Range
persistent identity: range1946931
display id: range1946931
version: 1
direction: direction/forward
top level: BBa_I739102
start: 50
end: 57
orientation: inline

TetR 1
type: SequenceAnnotation
persistent identity: annotation1946934
display id: annotation1946934
version: 1
top level: BBa_I739102
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I739102/annotation1946934/range1946934/1

https://synbiohub.org/public/igem/BBa_I739102/annotation1946934/range1946934/1
type: Range
persistent identity: range1946934
display id: range1946934
version: 1
direction: direction/forward
top level: BBa_I739102
start: 58
end: 77
orientation: inline

R0040 (2x TetR 1)
type: SequenceAnnotation
persistent identity: annotation1946933
display id: annotation1946933
version: 1
top level: BBa_I739102
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_I739102/annotation1946933/range1946933/1

https://synbiohub.org/public/igem/BBa_I739102/annotation1946933/range1946933/1
type: Range
persistent identity: range1946933
display id: range1946933
version: 1
direction: direction/forward
top level: BBa_I739102
start: 58
end: 97
orientation: inline

TetR 1
type: SequenceAnnotation
persistent identity: annotation1946932
display id: annotation1946932
version: 1
top level: BBa_I739102
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I739102/annotation1946932/range1946932/1

https://synbiohub.org/public/igem/BBa_I739102/annotation1946932/range1946932/1
type: Range
persistent identity: range1946932
display id: range1946932
version: 1
direction: direction/forward
top level: BBa_I739102
start: 78
end: 97
orientation: inline

R0051
type: SequenceAnnotation
persistent identity: annotation1946930
display id: annotation1946930
version: 1
top level: BBa_I739102
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I739102/annotation1946930/range1946930/1

https://synbiohub.org/public/igem/BBa_I739102/annotation1946930/range1946930/1
type: Range
persistent identity: range1946930
display id: range1946930
version: 1
direction: direction/forward
top level: BBa_I739102
start: 1
end: 49
orientation: inline

U061O11+D0
type: ComponentDefinition
persistent identity: BBa I732434
display id: BBa_I732434
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member (U061O11+D038O22)
creator: Zhan Jian
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732434_sequence/1

U049O11+D0
type: ComponentDefinition
persistent identity: BBa I732442
display id: BBa_I732442
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member (U049O11+D062O22)
creator: Zhan Jian
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732442_sequence/1

BBa_I13453
type: ComponentDefinition
persistent identity: BBa I13453
display id: BBa_I13453
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Pbad promoter
creator: jkm
created: 2005-05-24T11:00:00Z
modified: 2015-08-31T04:07:34Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _11_
m user id: 0
owner id: 253
owning group id: 6
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: PBad promoter from I0500 without AraC.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I13453_sequence/1

Pnot(O24)
type: ComponentDefinition
persistent identity: BBa I732274
display id: BBa_I732274
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member with Hybrid Operator (D001O24)
creator: Zhan Jian
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:57Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732274_sequence/1

BBa_K266004
type: ComponentDefinition
persistent identity: BBa K266004
display id: BBa_K266004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Const Lac Inverter -> LasR
creator: UNAM FCIENCIAS-CINVESTAV IRA
created: 2009-10-12T11:00:00Z
modified: 2015-05-08T01:11:43Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _363_
m user id: 0
owner id: 1955
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: Constitutive promoter J23100 with Lac system inverter controlling the expression of LasR
mutable notes: None
mutable provenance: The biobricks BBa_J23100 and BBa_K266003 where used for build this biobrick
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Inverter
component: BBa_J23100
component: BBa_K266003
sequence annotation: BBa_K266003
sequence annotation: BBa_J23100
sequence: https://synbiohub.org/public/igem/BBa_K266004_sequence/1

BBa_J23100
type: Component
persistent identity: component2287678
display id: component2287678
version: 1
top level: BBa_K266004
access: public
definition: BBa_J23100

BBa_K266003
type: Component
persistent identity: component2287713
display id: component2287713
version: 1
top level: BBa_K266004
access: public
definition: BBa_K266003

BBa_K266003
type: SequenceAnnotation
persistent identity: annotation2287713
display id: annotation2287713
version: 1
top level: BBa_K266004
location: https://synbiohub.org/public/igem/BBa_K266004/annotation2287713/range2287713/1
component: BBa_K266003

https://synbiohub.org/public/igem/BBa_K266004/annotation2287713/range2287713/1
type: Range
persistent identity: range2287713
display id: range2287713
version: 1
direction: direction/reverse
top level: BBa_K266004
start: 44
end: 2358
orientation: inline

BBa_J23100
type: SequenceAnnotation
persistent identity: annotation2287678
display id: annotation2287678
version: 1
top level: BBa_K266004
location: https://synbiohub.org/public/igem/BBa_K266004/annotation2287678/range2287678/1
component: BBa_J23100

https://synbiohub.org/public/igem/BBa_K266004/annotation2287678/range2287678/1
type: Range
persistent identity: range2287678
display id: range2287678
version: 1
direction: direction/reverse
top level: BBa_K266004
start: 1
end: 35
orientation: inline

BBa_R2113
type: ComponentDefinition
persistent identity: BBa R2113
display id: BBa_R2113
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter with operator site for C2003
creator: Reshma Shetty
created: 2006-10-20T11:00:00Z
modified: 2015-05-08T01:14:16Z
discontinued: false
dominant: false
group u list: _41_
m user id: 0
owner id: 126
owning group id: 70
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This promoter is designed to bind to C2003.
mutable notes: The placement of the operator site was designed to be near optimal for steric interference between C2003 and RNA polymerase.
mutable provenance: This promoter was designed rationally.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -10 site
sequence annotation: transcription start site
sequence annotation: C2003 operator site
sequence annotation: -35 site
sequence: https://synbiohub.org/public/igem/BBa_R2113_sequence/1

-10 site
type: SequenceAnnotation
persistent identity: annotation1904656
display id: annotation1904656
version: 1
top level: BBa_R2113
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R2113/annotation1904656/range1904656/1

https://synbiohub.org/public/igem/BBa_R2113/annotation1904656/range1904656/1
type: Range
persistent identity: range1904656
display id: range1904656
version: 1
direction: direction/forward
top level: BBa_R2113
start: 40
end: 45
orientation: inline

transcription start site
type: SequenceAnnotation
persistent identity: annotation1904657
display id: annotation1904657
version: 1
top level: BBa_R2113
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_R2113/annotation1904657/range1904657/1

https://synbiohub.org/public/igem/BBa_R2113/annotation1904657/range1904657/1
type: Range
persistent identity: range1904657
display id: range1904657
version: 1
direction: direction/forward
top level: BBa_R2113
start: 53
end: 53
orientation: inline

C2003 operator site
type: SequenceAnnotation
persistent identity: annotation1904658
display id: annotation1904658
version: 1
top level: BBa_R2113
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_R2113/annotation1904658/range1904658/1

https://synbiohub.org/public/igem/BBa_R2113/annotation1904658/range1904658/1
type: Range
persistent identity: range1904658
display id: range1904658
version: 1
direction: direction/forward
top level: BBa_R2113
start: 21
end: 32
orientation: inline

-35 site
type: SequenceAnnotation
persistent identity: annotation1904655
display id: annotation1904655
version: 1
top level: BBa_R2113
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R2113/annotation1904655/range1904655/1

https://synbiohub.org/public/igem/BBa_R2113/annotation1904655/range1904655/1
type: Range
persistent identity: range1904655
display id: range1904655
version: 1
direction: direction/forward
top level: BBa_R2113
start: 17
end: 22
orientation: inline

BBa_K091117
type: ComponentDefinition
persistent identity: BBa K091117
display id: BBa_K091117
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pLas promoter
creator: Erin Feeney
created: 2008-06-25T11:00:00Z
modified: 2015-05-08T01:08:37Z
discontinued: false
dominant: true
experience: experience/None
group u list: _191_
m user id: 0
owner id: 3067
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This pLas' promoter contains the regulatory region upstream of the LasI gene in Pseudomonas aeruginosa. K091117 includes a LasR binding site as well as a -10 region modified from CATAAA to TATAAA to reflect our pLasXOR promoter design. Activation is expected to occur in the presence of the autoinducer PAI-1 and the LasR protein. The pLas' promoter serves as a control for the evaluation of the pLasXOR promoter, part BBa_K091118.
mutable notes: To minimize leaky transcription, a secondary transcriptional start site was excluded from the design.
mutable provenance: The sequence of the LasI upstream regulatory region is available at http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=176640
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -10 Region
sequence annotation: -35 Region
sequence annotation: LasR Binding Site
sequence annotation: Primary Transcriptional Start
sequence: https://synbiohub.org/public/igem/BBa_K091117_sequence/1

-10 Region
type: SequenceAnnotation
persistent identity: annotation1964074
display id: annotation1964074
version: 1
top level: BBa_K091117
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K091117/annotation1964074/range1964074/1

https://synbiohub.org/public/igem/BBa_K091117/annotation1964074/range1964074/1
type: Range
persistent identity: range1964074
display id: range1964074
version: 1
direction: direction/forward
top level: BBa_K091117
start: 113
end: 118
orientation: inline

-35 Region
type: SequenceAnnotation
persistent identity: annotation1964072
display id: annotation1964072
version: 1
top level: BBa_K091117
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K091117/annotation1964072/range1964072/1

https://synbiohub.org/public/igem/BBa_K091117/annotation1964072/range1964072/1
type: Range
persistent identity: range1964072
display id: range1964072
version: 1
direction: direction/forward
top level: BBa_K091117
start: 86
end: 91
orientation: inline

LasR Binding Site
type: SequenceAnnotation
persistent identity: annotation1964073
display id: annotation1964073
version: 1
top level: BBa_K091117
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K091117/annotation1964073/range1964073/1

https://synbiohub.org/public/igem/BBa_K091117/annotation1964073/range1964073/1
type: Range
persistent identity: range1964073
display id: range1964073
version: 1
direction: direction/forward
top level: BBa_K091117
start: 75
end: 93
orientation: inline

Primary Transcriptional Start
type: SequenceAnnotation
persistent identity: annotation1964075
display id: annotation1964075
version: 1
top level: BBa_K091117
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K091117/annotation1964075/range1964075/1

https://synbiohub.org/public/igem/BBa_K091117/annotation1964075/range1964075/1
type: Range
persistent identity: range1964075
display id: range1964075
version: 1
direction: direction/reverse
top level: BBa_K091117
start: 125
end: 125
orientation: inline

BBa_K091157
type: ComponentDefinition
persistent identity: BBa K091157
display id: BBa_K091157
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pLux/Las Hybrid Promoter
creator: Erin Feeney
created: 2008-07-24T11:00:00Z
modified: 2015-05-08T01:08:38Z
discontinued: false
dominant: false
experience: experience/None
group u list: _191_
m user id: 0
owner id: 3067
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: K091157 is a modified version of the pLux' promoter (BBa_K091156) containing the addition of a LasR binding site between the -35 and -10 regions. This promoter is expected to be activated in the presence of AI-1 and LuxR and repressed in the presence of PAI-1 and LasR.
mutable notes: In order to fit the LasR binding site between the -35 and -10 regions, the last base of the Las Box was cropped and the -35 region was modified to overlap with the first two bases of the Las Box.
mutable provenance: The LasR binding site is from the region upstream of the LasI gene in Pseudomonas aeruginosa. The rest of the promoter is the same as BBa_K091156.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -10 Region
sequence annotation: LuxR+AI-1
sequence annotation: -35 Region
sequence annotation: LasR + PAI-1
sequence: https://synbiohub.org/public/igem/BBa_K091157_sequence/1

-10 Region
type: SequenceAnnotation
persistent identity: annotation1968424
display id: annotation1968424
version: 1
top level: BBa_K091157
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K091157/annotation1968424/range1968424/1

https://synbiohub.org/public/igem/BBa_K091157/annotation1968424/range1968424/1
type: Range
persistent identity: range1968424
display id: range1968424
version: 1
direction: direction/forward
top level: BBa_K091157
start: 42
end: 47
orientation: inline

LuxR+AI-1
type: SequenceAnnotation
persistent identity: annotation1968426
display id: annotation1968426
version: 1
top level: BBa_K091157
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K091157/annotation1968426/range1968426/1

https://synbiohub.org/public/igem/BBa_K091157/annotation1968426/range1968426/1
type: Range
persistent identity: range1968426
display id: range1968426
version: 1
direction: direction/forward
top level: BBa_K091157
start: 1
end: 20
orientation: inline

-35 Region
type: SequenceAnnotation
persistent identity: annotation1968423
display id: annotation1968423
version: 1
top level: BBa_K091157
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K091157/annotation1968423/range1968423/1

https://synbiohub.org/public/igem/BBa_K091157/annotation1968423/range1968423/1
type: Range
persistent identity: range1968423
display id: range1968423
version: 1
direction: direction/forward
top level: BBa_K091157
start: 20
end: 25
orientation: inline

LasR + PAI-1
type: SequenceAnnotation
persistent identity: annotation1968425
display id: annotation1968425
version: 1
top level: BBa_K091157
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K091157/annotation1968425/range1968425/1

https://synbiohub.org/public/igem/BBa_K091157/annotation1968425/range1968425/1
type: Range
persistent identity: range1968425
display id: range1968425
version: 1
direction: direction/forward
top level: BBa_K091157
start: 24
end: 42
orientation: inline

LacI
type: ComponentDefinition
persistent identity: BBa R0010
display id: BBa_R0010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: promoter (lacI regulated)
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) The pLac regulatory region is a 243 base-pair sequence with standard BioBrick prefix and suffix sections on its ends. It contains two protein binding sites: CAP, which is generally present in E.coli and is assocciated with cell health and availability of glucose., and LacI, the Lac inhibitor <bb_part>BBa_C0010</bb_part> which binds in an dimerized cooperative manner to inhibit the transcription of the protein that follows. In the presence of lactose or IPTG, an analog of lactose, LacI is unable to correctly bind and inhibit transcription. This allows <bb_part>BBa_R0010</bb_part> to be used as a inverter or as a detector of lactose or IPTG.
mutable notes: LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and this regulator. This part is incompatible with environments containing lactose or lactose analogs.
mutable provenance: The Plac insert was PCR'd from the MG1655 strain of E.coli K12.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: CAP binding site
sequence annotation: -35
sequence annotation: LacI binding site
sequence annotation: BBa_R0010
sequence annotation: end of LacI coding region (inactive)
sequence annotation: -10
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_R0010_sequence/1

CAP binding site
type: SequenceAnnotation
persistent identity: annotation1961223
display id: annotation1961223
version: 1
top level: LacI
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961223/range1961223/1

https://synbiohub.org/public/igem/BBa_R0010/annotation1961223/range1961223/1
type: Range
persistent identity: range1961223
display id: range1961223
version: 1
direction: direction/forward
top level: LacI
start: 89
end: 126
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1961224
display id: annotation1961224
version: 1
top level: LacI
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961224/range1961224/1

https://synbiohub.org/public/igem/BBa_R0010/annotation1961224/range1961224/1
type: Range
persistent identity: range1961224
display id: range1961224
version: 1
direction: direction/forward
top level: LacI
start: 137
end: 142
orientation: inline

LacI binding site
type: SequenceAnnotation
persistent identity: annotation1961226
display id: annotation1961226
version: 1
top level: LacI
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961226/range1961226/1

https://synbiohub.org/public/igem/BBa_R0010/annotation1961226/range1961226/1
type: Range
persistent identity: range1961226
display id: range1961226
version: 1
direction: direction/forward
top level: LacI
start: 166
end: 200
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation1961222
display id: annotation1961222
version: 1
top level: LacI
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961222/range1961222/1

https://synbiohub.org/public/igem/BBa_R0010/annotation1961222/range1961222/1
type: Range
persistent identity: range1961222
display id: range1961222
version: 1
direction: direction/forward
top level: LacI
start: 1
end: 200
orientation: inline

end of LacI coding region (inactive)
type: SequenceAnnotation
persistent identity: annotation1961221
display id: annotation1961221
version: 1
top level: LacI
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961221/range1961221/1

https://synbiohub.org/public/igem/BBa_R0010/annotation1961221/range1961221/1
type: Range
persistent identity: range1961221
display id: range1961221
version: 1
direction: direction/forward
top level: LacI
start: 1
end: 88
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1961225
display id: annotation1961225
version: 1
top level: LacI
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961225/range1961225/1

https://synbiohub.org/public/igem/BBa_R0010/annotation1961225/range1961225/1
type: Range
persistent identity: range1961225
display id: range1961225
version: 1
direction: direction/forward
top level: LacI
start: 161
end: 166
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation1961227
display id: annotation1961227
version: 1
top level: LacI
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961227/range1961227/1

https://synbiohub.org/public/igem/BBa_R0010/annotation1961227/range1961227/1
type: Range
persistent identity: range1961227
display id: range1961227
version: 1
direction: direction/forward
top level: LacI
start: 173
end: 173
orientation: inline

BBa_K137050
type: ComponentDefinition
persistent identity: BBa K137050
display id: BBa_K137050
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: 650 bp inverted tetR promoter
creator: Allen Lin
created: 2008-07-20T11:00:00Z
modified: 2015-05-08T01:10:09Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _187_
m user id: 0
owner id: 3112
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Inverted tetR promoter with noncoding, spacer DNA upstream of it to make the total length 650 bp.
mutable notes: This part is in a series of inverted tetR promoters that have total lengths of 150, 250, 350, 450, 650, and 850 bp. We chose the region upstream of tetR in part Q04400 to be the noncoding DNA segment. This noncoding segment consists of the 3' end of tetR and B0015. None of the parts in this series was long enough to include the start codon of tetR, so a functional tetR protein should not be transcribed.
mutable provenance: Primers were synthesized to bind to part Q04400, and then a PCR reaction was run.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: tetR promoter
sequence: https://synbiohub.org/public/igem/BBa_K137050_sequence/1

tetR promoter
type: SequenceAnnotation
persistent identity: annotation1968023
display id: annotation1968023
version: 1
top level: BBa_K137050
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K137050/annotation1968023/range1968023/1

https://synbiohub.org/public/igem/BBa_K137050/annotation1968023/range1968023/1
type: Range
persistent identity: range1968023
display id: range1968023
version: 1
direction: direction/reverse
top level: BBa_K137050
start: 1
end: 54
orientation: inline

lux pR-3
type: ComponentDefinition
persistent identity: BBa K658006
display id: BBa_K658006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: position 3 mutated promoter lux pR-3 (luxR & HSL regulated)
creator: Yidan Hu
created: 2011-09-30T11:00:00Z
modified: 2015-05-08T01:13:02Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _835_
m user id: 0
owner id: 10207
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: The position 3 mutated promoter lux pR-3 is a product of site-directed mutagenesis of promoter lux pR (BBa_R0062). The promoter lux pR is based on the Vibrio fischeri quorum sensing gene promoters. The position 3 mutated promoter lux pR-3 gives a higher expression of downstream genes in an autoinduction system. In another word, the lux pR-3 has a higher efficiency than the lux pR. Similar to promoter lux pR, the expression is up-regulated by the action of LuxR protein combined with the signal molecule HSL.
mutable notes: In the promoter lux pR's sequence, the C residue of position 3 was changed to a T using PCR method.
mutable provenance: The position 3 mutated promoter lux pR-3 is based on promoter lux pR (BBa_R0062).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -10
sequence annotation: -35
sequence annotation: LuxR/HSL
sequence annotation: start
sequence annotation: K658006
sequence: https://synbiohub.org/public/igem/BBa_K658006_sequence/1

-10
type: SequenceAnnotation
persistent identity: annotation2148162
display id: annotation2148162
version: 1
top level: lux pR-3
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K658006/annotation2148162/range2148162/1

https://synbiohub.org/public/igem/BBa_K658006/annotation2148162/range2148162/1
type: Range
persistent identity: range2148162
display id: range2148162
version: 1
direction: direction/forward
top level: lux pR-3
start: 42
end: 47
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2148161
display id: annotation2148161
version: 1
top level: lux pR-3
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K658006/annotation2148161/range2148161/1

https://synbiohub.org/public/igem/BBa_K658006/annotation2148161/range2148161/1
type: Range
persistent identity: range2148161
display id: range2148161
version: 1
direction: direction/forward
top level: lux pR-3
start: 20
end: 25
orientation: inline

LuxR/HSL
type: SequenceAnnotation
persistent identity: annotation2148166
display id: annotation2148166
version: 1
top level: lux pR-3
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K658006/annotation2148166/range2148166/1

https://synbiohub.org/public/igem/BBa_K658006/annotation2148166/range2148166/1
type: Range
persistent identity: range2148166
display id: range2148166
version: 1
direction: direction/forward
top level: lux pR-3
start: 1
end: 20
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2148163
display id: annotation2148163
version: 1
top level: lux pR-3
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K658006/annotation2148163/range2148163/1

https://synbiohub.org/public/igem/BBa_K658006/annotation2148163/range2148163/1
type: Range
persistent identity: range2148163
display id: range2148163
version: 1
direction: direction/forward
top level: lux pR-3
start: 53
end: 53
orientation: inline

K658006
type: SequenceAnnotation
persistent identity: annotation2148164
display id: annotation2148164
version: 1
top level: lux pR-3
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K658006/annotation2148164/range2148164/1

https://synbiohub.org/public/igem/BBa_K658006/annotation2148164/range2148164/1
type: Range
persistent identity: range2148164
display id: range2148164
version: 1
direction: direction/forward
top level: lux pR-3
start: 1
end: 55
orientation: inline

BBa_K121014
type: ComponentDefinition
persistent identity: BBa K121014
display id: BBa_K121014
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: promoter (lambda cI regulated)
creator: Masashi Kajita
created: 2008-10-15T11:00:00Z
modified: 2015-05-08T01:09:41Z
discontinued: false
dominant: false
group u list: _220_
m user id: 0
owner id: 3180
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: promoter (lambda cI regulated)
mutable notes: na
mutable provenance: na
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: K121014
sequence annotation: OL1
sequence annotation: -10
sequence annotation: -35
sequence annotation: OL3
sequence annotation: OL2
sequence: https://synbiohub.org/public/igem/BBa_K121014_sequence/1

K121014
type: SequenceAnnotation
persistent identity: annotation1981846
display id: annotation1981846
version: 1
top level: BBa_K121014
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K121014/annotation1981846/range1981846/1

https://synbiohub.org/public/igem/BBa_K121014/annotation1981846/range1981846/1
type: Range
persistent identity: range1981846
display id: range1981846
version: 1
direction: direction/forward
top level: BBa_K121014
start: 1
end: 90
orientation: inline

OL1
type: SequenceAnnotation
persistent identity: annotation1981849
display id: annotation1981849
version: 1
top level: BBa_K121014
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K121014/annotation1981849/range1981849/1

https://synbiohub.org/public/igem/BBa_K121014/annotation1981849/range1981849/1
type: Range
persistent identity: range1981849
display id: range1981849
version: 1
direction: direction/forward
top level: BBa_K121014
start: 57
end: 73
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1981850
display id: annotation1981850
version: 1
top level: BBa_K121014
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K121014/annotation1981850/range1981850/1

https://synbiohub.org/public/igem/BBa_K121014/annotation1981850/range1981850/1
type: Range
persistent identity: range1981850
display id: range1981850
version: 1
direction: direction/forward
top level: BBa_K121014
start: 70
end: 75
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1981851
display id: annotation1981851
version: 1
top level: BBa_K121014
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K121014/annotation1981851/range1981851/1

https://synbiohub.org/public/igem/BBa_K121014/annotation1981851/range1981851/1
type: Range
persistent identity: range1981851
display id: range1981851
version: 1
direction: direction/forward
top level: BBa_K121014
start: 47
end: 52
orientation: inline

OL3
type: SequenceAnnotation
persistent identity: annotation1981847
display id: annotation1981847
version: 1
top level: BBa_K121014
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K121014/annotation1981847/range1981847/1

https://synbiohub.org/public/igem/BBa_K121014/annotation1981847/range1981847/1
type: Range
persistent identity: range1981847
display id: range1981847
version: 1
direction: direction/forward
top level: BBa_K121014
start: 13
end: 29
orientation: inline

OL2
type: SequenceAnnotation
persistent identity: annotation1981848
display id: annotation1981848
version: 1
top level: BBa_K121014
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K121014/annotation1981848/range1981848/1

https://synbiohub.org/public/igem/BBa_K121014/annotation1981848/range1981848/1
type: Range
persistent identity: range1981848
display id: range1981848
version: 1
direction: direction/forward
top level: BBa_K121014
start: 33
end: 49
orientation: inline

U097O11+D0
type: ComponentDefinition
persistent identity: BBa I732413
display id: BBa_I732413
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member (U097O11+D002O22)
creator: Zhan Jian
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732413_sequence/1

U097O11+D0
type: ComponentDefinition
persistent identity: BBa I732418
display id: BBa_I732418
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member (U097O11+D062O22)
creator: Zhan Jian
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732418_sequence/1

BBa_K094140
type: ComponentDefinition
persistent identity: BBa K094140
display id: BBa_K094140
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pLacIq
creator: Liu Chenli
created: 2008-10-12T11:00:00Z
modified: 2015-05-08T01:08:40Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _255_
m user id: 0
owner id: 2589
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: mutant promoter of lacI gene, which enables high level of lacI protein expression
mutable notes: no specific considerations
mutable provenance: point mutation made from E. Coli genomic lacI gene promoter
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: pLacIq
sequence: https://synbiohub.org/public/igem/BBa_K094140_sequence/1

pLacIq
type: SequenceAnnotation
persistent identity: annotation1981123
display id: annotation1981123
version: 1
top level: BBa_K094140
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K094140/annotation1981123/range1981123/1

https://synbiohub.org/public/igem/BBa_K094140/annotation1981123/range1981123/1
type: Range
persistent identity: range1981123
display id: range1981123
version: 1
direction: direction/forward
top level: BBa_K094140
start: 1
end: 80
orientation: inline

BBa_I751500
type: ComponentDefinition
persistent identity: BBa I751500
display id: BBa_I751500
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pcI (for positive control of pcI-lux hybrid promoter)
creator: Kenichiro Iwasaki
created: 2007-10-24T11:00:00Z
modified: 2015-08-31T04:08:06Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _151_
m user id: 0
owner id: 2188
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: pcI
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: OR2
sequence annotation: pcI
sequence annotation: -35
sequence annotation: OR1
sequence annotation: -10
sequence annotation: OR3
sequence: https://synbiohub.org/public/igem/BBa_I751500_sequence/1

OR2
type: SequenceAnnotation
persistent identity: annotation1958483
display id: annotation1958483
version: 1
top level: BBa_I751500
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I751500/annotation1958483/range1958483/1

https://synbiohub.org/public/igem/BBa_I751500/annotation1958483/range1958483/1
type: Range
persistent identity: range1958483
display id: range1958483
version: 1
direction: direction/forward
top level: BBa_I751500
start: 27
end: 46
orientation: inline

pcI
type: SequenceAnnotation
persistent identity: annotation1955476
display id: annotation1955476
version: 1
top level: BBa_I751500
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I751500/annotation1955476/range1955476/1

https://synbiohub.org/public/igem/BBa_I751500/annotation1955476/range1955476/1
type: Range
persistent identity: range1955476
display id: range1955476
version: 1
direction: direction/forward
top level: BBa_I751500
start: 1
end: 77
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1958479
display id: annotation1958479
version: 1
top level: BBa_I751500
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I751500/annotation1958479/range1958479/1

https://synbiohub.org/public/igem/BBa_I751500/annotation1958479/range1958479/1
type: Range
persistent identity: range1958479
display id: range1958479
version: 1
direction: direction/forward
top level: BBa_I751500
start: 21
end: 26
orientation: inline

OR1
type: SequenceAnnotation
persistent identity: annotation1958484
display id: annotation1958484
version: 1
top level: BBa_I751500
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I751500/annotation1958484/range1958484/1

https://synbiohub.org/public/igem/BBa_I751500/annotation1958484/range1958484/1
type: Range
persistent identity: range1958484
display id: range1958484
version: 1
direction: direction/forward
top level: BBa_I751500
start: 51
end: 77
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1958480
display id: annotation1958480
version: 1
top level: BBa_I751500
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I751500/annotation1958480/range1958480/1

https://synbiohub.org/public/igem/BBa_I751500/annotation1958480/range1958480/1
type: Range
persistent identity: range1958480
display id: range1958480
version: 1
direction: direction/forward
top level: BBa_I751500
start: 47
end: 50
orientation: inline

OR3
type: SequenceAnnotation
persistent identity: annotation1958482
display id: annotation1958482
version: 1
top level: BBa_I751500
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I751500/annotation1958482/range1958482/1

https://synbiohub.org/public/igem/BBa_I751500/annotation1958482/range1958482/1
type: Range
persistent identity: range1958482
display id: range1958482
version: 1
direction: direction/forward
top level: BBa_I751500
start: 1
end: 20
orientation: inline

BBa_K137032
type: ComponentDefinition
persistent identity: BBa K137032
display id: BBa_K137032
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: constitutive promoter with (C)12 between -10 and -35 elements
creator: Allen Lin
created: 2008-07-20T11:00:00Z
modified: 2015-05-08T01:10:09Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _187_
m user id: 0
owner id: 3112
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: constitutive promoter with (C)12 between -10 and -35 elements
mutable notes: With 12 C repeats between the -10 and -35 elements, the distance between the two elements is optimal for the sigma factor to bind to the promoter. Thus, the promoter should be in the 'on' state.
mutable provenance: This part was PCR amplified from two synthesized primers that bound to each other.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -10 element
sequence annotation: c repeat
sequence: https://synbiohub.org/public/igem/BBa_K137032_sequence/1

-10 element
type: SequenceAnnotation
persistent identity: annotation1967773
display id: annotation1967773
version: 1
top level: BBa_K137032
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K137032/annotation1967773/range1967773/1

https://synbiohub.org/public/igem/BBa_K137032/annotation1967773/range1967773/1
type: Range
persistent identity: range1967773
display id: range1967773
version: 1
direction: direction/forward
top level: BBa_K137032
start: 52
end: 57
orientation: inline

c repeat
type: SequenceAnnotation
persistent identity: annotation1967774
display id: annotation1967774
version: 1
top level: BBa_K137032
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K137032/annotation1967774/range1967774/1

https://synbiohub.org/public/igem/BBa_K137032/annotation1967774/range1967774/1
type: Range
persistent identity: range1967774
display id: range1967774
version: 1
direction: direction/forward
top level: BBa_K137032
start: 27
end: 38
orientation: inline

BBa_J102001
type: ComponentDefinition
persistent identity: BBa J102001
display id: BBa_J102001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Reverse Lux Promoter
creator: Michael Pettigrew
created: 2010-07-01T11:00:00Z
modified: 2015-08-31T04:08:24Z
discontinued: false
dominant: false
group u list: _580_
m user id: 0
owner id: 5944
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This promoter is the right promoter in the lux cassette of Vibrio Fischeri. There is weak constitutive expression of downstream genes. Expression of downstream genes can be up-regulated by the action of the LuxR activator protein in complex with the autoinducer, 3-oxo-hexanoyl-HSL. Two molecules of LuxR protein can form a complex with two molecules of the AHL. This causes a conformational change in the protein, allowing it to bind to a site on the lux promoter and induce transcription.
mutable notes: None.
mutable provenance: This part was obtained by taking the reverse complement of R0062, ordering appropriate oligos, then annealing and extending them.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: promoter
sequence: https://synbiohub.org/public/igem/BBa_J102001_sequence/1

promoter
type: SequenceAnnotation
persistent identity: annotation2072374
display id: annotation2072374
version: 1
top level: BBa_J102001
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J102001/annotation2072374/range2072374/1

https://synbiohub.org/public/igem/BBa_J102001/annotation2072374/range2072374/1
type: Range
persistent identity: range2072374
display id: range2072374
version: 1
direction: direction/reverse
top level: BBa_J102001
start: 1
end: 55
orientation: inline

BBa_R2112
type: ComponentDefinition
persistent identity: BBa R2112
display id: BBa_R2112
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter with operator site for C2003
creator: Reshma Shetty
created: 2006-10-20T11:00:00Z
modified: 2015-05-08T01:14:16Z
discontinued: false
dominant: false
group u list: _41_
m user id: 0
owner id: 126
owning group id: 70
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This promoter is designed to bind to C2003.
mutable notes: The placement of the operator site was designed to be near optimal for steric interference between C2003 and RNA polymerase.
mutable provenance: This promoter was rationally designed.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -10 site
sequence annotation: transcription start site
sequence annotation: -35 site
sequence annotation: C2003 operator site
sequence: https://synbiohub.org/public/igem/BBa_R2112_sequence/1

-10 site
type: SequenceAnnotation
persistent identity: annotation1903925
display id: annotation1903925
version: 1
top level: BBa_R2112
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R2112/annotation1903925/range1903925/1

https://synbiohub.org/public/igem/BBa_R2112/annotation1903925/range1903925/1
type: Range
persistent identity: range1903925
display id: range1903925
version: 1
direction: direction/forward
top level: BBa_R2112
start: 40
end: 45
orientation: inline

transcription start site
type: SequenceAnnotation
persistent identity: annotation1903926
display id: annotation1903926
version: 1
top level: BBa_R2112
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_R2112/annotation1903926/range1903926/1

https://synbiohub.org/public/igem/BBa_R2112/annotation1903926/range1903926/1
type: Range
persistent identity: range1903926
display id: range1903926
version: 1
direction: direction/forward
top level: BBa_R2112
start: 53
end: 53
orientation: inline

-35 site
type: SequenceAnnotation
persistent identity: annotation1903924
display id: annotation1903924
version: 1
top level: BBa_R2112
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R2112/annotation1903924/range1903924/1

https://synbiohub.org/public/igem/BBa_R2112/annotation1903924/range1903924/1
type: Range
persistent identity: range1903924
display id: range1903924
version: 1
direction: direction/forward
top level: BBa_R2112
start: 17
end: 22
orientation: inline

C2003 operator site
type: SequenceAnnotation
persistent identity: annotation1903927
display id: annotation1903927
version: 1
top level: BBa_R2112
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_R2112/annotation1903927/range1903927/1

https://synbiohub.org/public/igem/BBa_R2112/annotation1903927/range1903927/1
type: Range
persistent identity: range1903927
display id: range1903927
version: 1
direction: direction/forward
top level: BBa_R2112
start: 31
end: 42
orientation: inline

BBa_K091106
type: ComponentDefinition
persistent identity: BBa K091106
display id: BBa_K091106
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LsrA/cI hybrid promoter
creator: Andrew Gordon
created: 2008-06-18T11:00:00Z
modified: 2015-05-08T01:08:37Z
discontinued: false
dominant: false
experience: experience/None
group u list: _191_
m user id: 0
owner id: 3129
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This hybrid promoter uses the LsrA promoter that is capable of being repressed by LsrR - induction can occur with phospho-AI-2. Binding sites for cI repressor also occur. As a result the promoter is off in the absence of AI-2 and on in the presence of AI-2, but always off in the presence of cI.
mutable notes: The promoter region for LsrA should remain unaltered while cI binding sites (OR1 and OR2) are introduced downstream. The OR2 sites with cI bound should not allow transcription. The cI binding sites have a natural 6 bp spacer between them. It would be desirable to position the cI binding sites immediately downstream of the -10 region of the LsrA promoter. However, experimental evidence for the transciption start (+1) appears to be lacking.
mutable provenance: The Missouri Western/Davidson team designed primers for LsrA and extracted the DNA from E. coli MG1655. The CI portion was pulled from the registry as part number R0051.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K091106_sequence/1

BBa_K1585102
type: ComponentDefinition
persistent identity: BBa K1585102
display id: BBa_K1585102
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Anderson Promoter with lacI binding site
creator: Tobias Schwanemann
created: 2015-08-31T11:00:00Z
modified: 2015-09-01T02:03:57Z
discontinued: false
dominant: false
experience: experience/Fails
group u list: _2002_
m user id: 24474
owner id: 24474
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Promoters of the Anderson Promoter Collection with lacI binding site derived from [http://parts.igem.org/Part:BBa_R0010 R_0010] to make them [http://openwetware.org/wiki/IPTG IPTG] iducible.
mutable notes: We choosed lacI-binding-site of part BBa_R0010 because we assumed older parts would be more reliable. The part is shipped in backbone [http://parts.igem.org/Part:BBa_J61002 J61002] because the functionality is directly visible by adding IPTG. RFP ([http://parts.igem.org/Part:BBa_E1010 E1010]) should get expressed then.
mutable provenance: Assambly of [http://parts.igem.org/Part:BBa_J23102 J23102] and lacI-binding-site of [http://parts.igem.org/Part:BBa_R0010 R_0010] The "lacI binding site" of Part [http://parts.igem.org/Part:BBa_R0010 R_0010] differs from lacO from Part [http://parts.igem.org/Part:BBa_J33207 BBa_J33207].
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
component: BBa_K1585999
component: BBa_J23102
sequence annotation: BBa_J23102
sequence annotation: BBa_K1585999
sequence: https://synbiohub.org/public/igem/BBa_K1585102_sequence/1

BBa_K1585999
type: Component
persistent identity: component2441419
display id: component2441419
version: 1
top level: BBa_K1585102
access: public
definition: https://synbiohub.org/public/igem/BBa_K1585999/1

BBa_J23102
type: Component
persistent identity: component2441417
display id: component2441417
version: 1
top level: BBa_K1585102
access: public
definition: BBa_J23102

BBa_J23102
type: SequenceAnnotation
persistent identity: annotation2441417
display id: annotation2441417
version: 1
top level: BBa_K1585102
location: https://synbiohub.org/public/igem/BBa_K1585102/annotation2441417/range2441417/1
component: BBa_J23102

https://synbiohub.org/public/igem/BBa_K1585102/annotation2441417/range2441417/1
type: Range
persistent identity: range2441417
display id: range2441417
version: 1
direction: direction/reverse
top level: BBa_K1585102
start: 1
end: 35
orientation: inline

BBa_K1585999
type: SequenceAnnotation
persistent identity: annotation2441419
display id: annotation2441419
version: 1
top level: BBa_K1585102
location: https://synbiohub.org/public/igem/BBa_K1585102/annotation2441419/range2441419/1
component: BBa_K1585999

https://synbiohub.org/public/igem/BBa_K1585102/annotation2441419/range2441419/1
type: Range
persistent identity: range2441419
display id: range2441419
version: 1
direction: direction/reverse
top level: BBa_K1585102
start: 44
end: 78
orientation: inline

BBa_K137090
type: ComponentDefinition
persistent identity: BBa K137090
display id: BBa_K137090
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: optimized (A) repeat constitutive promoter with 17 bp between -10 and -35 elements
creator: Allen Lin
created: 2008-08-20T11:00:00Z
modified: 2015-05-08T01:10:10Z
discontinued: false
dominant: false
experience: experience/None
group u list: _187_
m user id: 0
owner id: 3112
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: optimized (A) repeat constitutive promoter with 17 bp between -10 and -35 elements
mutable notes: None
mutable provenance: PCR
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K137090_sequence/1

BBa_K094002
type: ComponentDefinition
persistent identity: BBa K094002
display id: BBa_K094002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: plambda P(O-R12)
creator: Shi Lei
created: 2008-07-01T11:00:00Z
modified: 2015-05-08T01:08:40Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _255_
m user id: 0
owner id: 2053
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: just for testing
mutable notes: just for testing
mutable provenance: just for testing
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: promoter
sequence: https://synbiohub.org/public/igem/BBa_K094002_sequence/1

promoter
type: SequenceAnnotation
persistent identity: annotation1985278
display id: annotation1985278
version: 1
top level: BBa_K094002
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K094002/annotation1985278/range1985278/1

https://synbiohub.org/public/igem/BBa_K094002/annotation1985278/range1985278/1
type: Range
persistent identity: range1985278
display id: range1985278
version: 1
direction: direction/forward
top level: BBa_K094002
start: 1
end: 100
orientation: inline

Pnot(O66)
type: ComponentDefinition
persistent identity: BBa I732206
display id: BBa_I732206
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: NOT Gate Promoter Family Member (D001O66)
creator: Zhan Jian
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:57Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732206_sequence/1

BBa_K259007
type: ComponentDefinition
persistent identity: BBa K259007
display id: BBa_K259007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: AraC Promoter fused with RBS
creator: Petros Mina
created: 2009-09-08T11:00:00Z
modified: 2015-05-08T01:11:42Z
discontinued: false
dominant: false
group u list: _357_
m user id: 0
owner id: 5318
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part has the arabinose regulated promoter (AraC/pBAD) upstream of a ribosome binding site. This is ideal for inserting a protein to be expressed downstream of this part as it's expression can be regulated by the presence of the concentration of arabinose in the growing environment/media of the cells. ==What this part actually does== This composite part includes the AraC/pBAD promoter and a strong ribosome binding site designed from the Anderson family of RBS. The AraC/pBAD promoter can be used with prokaryotic chassis to regulate expresion of proteins. When the AraC/pBAD promoter is placed upstream of your canditate translational unit (protein to be expressed) it allows control of the trancription of the said protein. This is done via controlling the concentration of arabinose sugar in the environment/solution of bacterial growth. Controlling the transcription will not ensure translation. The ribosome binding site is ideally placed a small distance away from the promoter site to ensure that the ribosome will bind and start translating the protein coding sequence found downstream.
mutable notes: The part was designed with RFC10 assembly from supplied biobricks of the iGEM2009 distribution.
mutable provenance: The AraC promoter can be found naturally at 1.52min of the E.coli genome ([http://ecogene.org/geneinfo.php?eg_id=EG10054 EcoGene number EG10054]. The RBS was taken from the designed sequences of RBS by the Anderson family. For more information visit the original part page [http://partsregistry.org/Part:BBa_J61100 here].
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
component: BBa_R0081
component: BBa_R0080
component: BBa_J61100
sequence annotation: BBa_R0081
sequence annotation: BBa_R0080
sequence annotation: BBa_J61100
sequence: https://synbiohub.org/public/igem/BBa_K259007_sequence/1

BBa_R0081
type: Component
persistent identity: component2020539
display id: component2020539
version: 1
top level: BBa_K259007
access: public
definition: https://synbiohub.org/public/igem/BBa_R0081/1

BBa_R0080
type: Component
persistent identity: component2020546
display id: component2020546
version: 1
top level: BBa_K259007
access: public
definition: AraC

BBa_J61100
type: Component
persistent identity: component2020547
display id: component2020547
version: 1
top level: BBa_K259007
access: public
definition: https://synbiohub.org/public/igem/BBa_J61100/1

BBa_R0081
type: SequenceAnnotation
persistent identity: annotation2020539
display id: annotation2020539
version: 1
top level: BBa_K259007
location: https://synbiohub.org/public/igem/BBa_K259007/annotation2020539/range2020539/1
component: BBa_R0081

https://synbiohub.org/public/igem/BBa_K259007/annotation2020539/range2020539/1
type: Range
persistent identity: range2020539
display id: range2020539
version: 1
direction: direction/reverse
top level: BBa_K259007
start: 1
end: 183
orientation: inline

BBa_R0080
type: SequenceAnnotation
persistent identity: annotation2020546
display id: annotation2020546
version: 1
top level: BBa_K259007
location: https://synbiohub.org/public/igem/BBa_K259007/annotation2020546/range2020546/1
component: BBa_R0080

https://synbiohub.org/public/igem/BBa_K259007/annotation2020546/range2020546/1
type: Range
persistent identity: range2020546
display id: range2020546
version: 1
direction: direction/reverse
top level: BBa_K259007
start: 192
end: 340
orientation: inline

BBa_J61100
type: SequenceAnnotation
persistent identity: annotation2020547
display id: annotation2020547
version: 1
top level: BBa_K259007
location: https://synbiohub.org/public/igem/BBa_K259007/annotation2020547/range2020547/1
component: BBa_J61100

https://synbiohub.org/public/igem/BBa_K259007/annotation2020547/range2020547/1
type: Range
persistent identity: range2020547
display id: range2020547
version: 1
direction: direction/reverse
top level: BBa_K259007
start: 349
end: 360
orientation: inline

BBa_J54130
type: ComponentDefinition
persistent identity: BBa J54130
display id: BBa_J54130
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: BetI_regulated promoter
creator: Yusaku Nakashima
created: 2006-10-20T11:00:00Z
modified: 2015-08-31T03:48:12Z
discontinued: false
dominant: false
group u list: _76_
m user id: 0
owner id: 762
owning group id: 76
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: BetI_regulated promoter
mutable notes: As BetI regulated
mutable provenance: DNA synthsis
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BamHI
sequence annotation: BetI_regulated promoter
sequence: https://synbiohub.org/public/igem/BBa_J54130_sequence/1

BamHI
type: SequenceAnnotation
persistent identity: annotation1903813
display id: annotation1903813
version: 1
top level: BBa_J54130
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_J54130/annotation1903813/range1903813/1

https://synbiohub.org/public/igem/BBa_J54130/annotation1903813/range1903813/1
type: Range
persistent identity: range1903813
display id: range1903813
version: 1
direction: direction/forward
top level: BBa_J54130
start: 1
end: 5
orientation: inline

BetI_regulated promoter
type: SequenceAnnotation
persistent identity: annotation1903812
display id: annotation1903812
version: 1
top level: BBa_J54130
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_J54130/annotation1903812/range1903812/1

https://synbiohub.org/public/igem/BBa_J54130/annotation1903812/range1903812/1
type: Range
persistent identity: range1903812
display id: range1903812
version: 1
direction: direction/forward
top level: BBa_J54130
start: 6
end: 43
orientation: inline

BBa_K338029
type: ComponentDefinition
persistent identity: BBa K338029
display id: BBa_K338029
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: +OmpR, +(CinR-HSL) Double Promoter
creator: Lucas Hartsough
created: 2010-10-26T11:00:00Z
modified: 2015-05-08T01:12:07Z
discontinued: false
dominant: false
group u list: _454_
m user id: 0
owner id: 6013
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: An intermediate part designed to be used with the AND gate designed by Caltech iGEM '10: [http://2010.igem.org/Team:Caltech/Project Project Details].
mutable notes: Designed to be placed upstream of positive feedback mechanisms and desired output genes.
mutable provenance: The Registry of Standard Biological Parts
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_R0082
component: BBa_R0077
sequence annotation: BBa_R0082
sequence annotation: BBa_R0077
sequence: https://synbiohub.org/public/igem/BBa_K338029_sequence/1

BBa_R0082
type: Component
persistent identity: component2104977
display id: component2104977
version: 1
top level: BBa_K338029
access: public
definition: OmpR

BBa_R0077
type: Component
persistent identity: component2104982
display id: component2104982
version: 1
top level: BBa_K338029
access: public
definition: https://synbiohub.org/public/igem/BBa_R0077/1

BBa_R0082
type: SequenceAnnotation
persistent identity: annotation2104977
display id: annotation2104977
version: 1
top level: BBa_K338029
location: https://synbiohub.org/public/igem/BBa_K338029/annotation2104977/range2104977/1
component: BBa_R0082

https://synbiohub.org/public/igem/BBa_K338029/annotation2104977/range2104977/1
type: Range
persistent identity: range2104977
display id: range2104977
version: 1
direction: direction/reverse
top level: BBa_K338029
start: 1
end: 108
orientation: inline

BBa_R0077
type: SequenceAnnotation
persistent identity: annotation2104982
display id: annotation2104982
version: 1
top level: BBa_K338029
location: https://synbiohub.org/public/igem/BBa_K338029/annotation2104982/range2104982/1
component: BBa_R0077

https://synbiohub.org/public/igem/BBa_K338029/annotation2104982/range2104982/1
type: Range
persistent identity: range2104982
display id: range2104982
version: 1
direction: direction/reverse
top level: BBa_K338029
start: 117
end: 347
orientation: inline

U097NUL+D0
type: ComponentDefinition
persistent identity: BBa I732407
display id: BBa_I732407
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member (U097NUL+D002O22)
creator: Zhan Jian
created: 2007-09-19T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1572
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: A Double Reporter (LacZ-alpha and GFP-AAV) loaded on pSB3C5. For more information please refer to BBa_I732401.
mutable notes: Might be later edited.
mutable provenance: Might be later edited.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BBa_B0012
sequence annotation: BBa_B0034
sequence annotation: BBa_I732007
sequence annotation: stem_loop
sequence annotation: BglII
sequence annotation: stop
sequence annotation: conserved
sequence annotation: STOP
sequence annotation: START
sequence annotation: T7 TE
sequence annotation: BBa_I732006
sequence annotation: polya
sequence annotation: BBa_B0010
sequence annotation: BamHI
sequence annotation: BBa_E0044
sequence annotation: lacZ
sequence: https://synbiohub.org/public/igem/BBa_I732407_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1944493
display id: annotation1944493
version: 1
top level: U097NUL+D0
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I732407/annotation1944493/range1944493/1

https://synbiohub.org/public/igem/BBa_I732407/annotation1944493/range1944493/1
type: Range
persistent identity: range1944493
display id: range1944493
version: 1
direction: direction/forward
top level: U097NUL+D0
start: 1161
end: 1201
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1944489
display id: annotation1944489
version: 1
top level: U097NUL+D0
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I732407/annotation1944489/range1944489/1

https://synbiohub.org/public/igem/BBa_I732407/annotation1944489/range1944489/1
type: Range
persistent identity: range1944489
display id: range1944489
version: 1
direction: direction/reverse
top level: U097NUL+D0
start: 288
end: 299
orientation: inline

BBa_I732007
type: SequenceAnnotation
persistent identity: annotation1944481
display id: annotation1944481
version: 1
top level: U097NUL+D0
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I732407/annotation1944481/range1944481/1

https://synbiohub.org/public/igem/BBa_I732407/annotation1944481/range1944481/1
type: Range
persistent identity: range1944481
display id: range1944481
version: 1
direction: direction/reverse
top level: U097NUL+D0
start: 1
end: 19
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation1944492
display id: annotation1944492
version: 1
top level: U097NUL+D0
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_I732407/annotation1944492/range1944492/1

https://synbiohub.org/public/igem/BBa_I732407/annotation1944492/range1944492/1
type: Range
persistent identity: range1944492
display id: range1944492
version: 1
direction: direction/forward
top level: U097NUL+D0
start: 1084
end: 1127
orientation: inline

BglII
type: SequenceAnnotation
persistent identity: annotation1944479
display id: annotation1944479
version: 1
top level: U097NUL+D0
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I732407/annotation1944479/range1944479/1

https://synbiohub.org/public/igem/BBa_I732407/annotation1944479/range1944479/1
type: Range
persistent identity: range1944479
display id: range1944479
version: 1
direction: direction/forward
top level: U097NUL+D0
start: 3
end: 8
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1944496
display id: annotation1944496
version: 1
top level: U097NUL+D0
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_I732407/annotation1944496/range1944496/1

https://synbiohub.org/public/igem/BBa_I732407/annotation1944496/range1944496/1
type: Range
persistent identity: range1944496
display id: range1944496
version: 1
direction: direction/forward
top level: U097NUL+D0
start: 1194
end: 1194
orientation: inline

conserved
type: SequenceAnnotation
persistent identity: annotation1944482
display id: annotation1944482
version: 1
top level: U097NUL+D0
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_I732407/annotation1944482/range1944482/1

https://synbiohub.org/public/igem/BBa_I732407/annotation1944482/range1944482/1
type: Range
persistent identity: range1944482
display id: range1944482
version: 1
direction: direction/forward
top level: U097NUL+D0
start: 32
end: 35
orientation: inline

STOP
type: SequenceAnnotation
persistent identity: annotation1944486
display id: annotation1944486
version: 1
top level: U097NUL+D0
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_I732407/annotation1944486/range1944486/1

https://synbiohub.org/public/igem/BBa_I732407/annotation1944486/range1944486/1
type: Range
persistent identity: range1944486
display id: range1944486
version: 1
direction: direction/forward
top level: U097NUL+D0
start: 274
end: 279
orientation: inline

START
type: SequenceAnnotation
persistent identity: annotation1944484
display id: annotation1944484
version: 1
top level: U097NUL+D0
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_I732407/annotation1944484/range1944484/1

https://synbiohub.org/public/igem/BBa_I732407/annotation1944484/range1944484/1
type: Range
persistent identity: range1944484
display id: range1944484
version: 1
direction: direction/forward
top level: U097NUL+D0
start: 46
end: 48
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1944494
display id: annotation1944494
version: 1
top level: U097NUL+D0
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_I732407/annotation1944494/range1944494/1

https://synbiohub.org/public/igem/BBa_I732407/annotation1944494/range1944494/1
type: Range
persistent identity: range1944494
display id: range1944494
version: 1
direction: direction/forward
top level: U097NUL+D0
start: 1168
end: 1187
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1944483
display id: annotation1944483
version: 1
top level: U097NUL+D0
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I732407/annotation1944483/range1944483/1

https://synbiohub.org/public/igem/BBa_I732407/annotation1944483/range1944483/1
type: Range
persistent identity: range1944483
display id: range1944483
version: 1
direction: direction/reverse
top level: U097NUL+D0
start: 28
end: 39
orientation: inline

BBa_I732006
type: SequenceAnnotation
persistent identity: annotation1944487
display id: annotation1944487
version: 1
top level: U097NUL+D0
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I732407/annotation1944487/range1944487/1

https://synbiohub.org/public/igem/BBa_I732407/annotation1944487/range1944487/1
type: Range
persistent identity: range1944487
display id: range1944487
version: 1
direction: direction/reverse
top level: U097NUL+D0
start: 46
end: 279
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1944495
display id: annotation1944495
version: 1
top level: U097NUL+D0
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_I732407/annotation1944495/range1944495/1

https://synbiohub.org/public/igem/BBa_I732407/annotation1944495/range1944495/1
type: Range
persistent identity: range1944495
display id: range1944495
version: 1
direction: direction/forward
top level: U097NUL+D0
start: 1188
end: 1201
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1944491
display id: annotation1944491
version: 1
top level: U097NUL+D0
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I732407/annotation1944491/range1944491/1

https://synbiohub.org/public/igem/BBa_I732407/annotation1944491/range1944491/1
type: Range
persistent identity: range1944491
display id: range1944491
version: 1
direction: direction/forward
top level: U097NUL+D0
start: 1073
end: 1152
orientation: inline

BamHI
type: SequenceAnnotation
persistent identity: annotation1944480
display id: annotation1944480
version: 1
top level: U097NUL+D0
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I732407/annotation1944480/range1944480/1

https://synbiohub.org/public/igem/BBa_I732407/annotation1944480/range1944480/1
type: Range
persistent identity: range1944480
display id: range1944480
version: 1
direction: direction/forward
top level: U097NUL+D0
start: 12
end: 17
orientation: inline

BBa_E0044
type: SequenceAnnotation
persistent identity: annotation1944490
display id: annotation1944490
version: 1
top level: U097NUL+D0
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I732407/annotation1944490/range1944490/1

https://synbiohub.org/public/igem/BBa_I732407/annotation1944490/range1944490/1
type: Range
persistent identity: range1944490
display id: range1944490
version: 1
direction: direction/reverse
top level: U097NUL+D0
start: 306
end: 1064
orientation: inline

lacZ
type: SequenceAnnotation
persistent identity: annotation1944485
display id: annotation1944485
version: 1
top level: U097NUL+D0
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_I732407/annotation1944485/range1944485/1

https://synbiohub.org/public/igem/BBa_I732407/annotation1944485/range1944485/1
type: Range
persistent identity: range1944485
display id: range1944485
version: 1
direction: direction/forward
top level: U097NUL+D0
start: 46
end: 279
orientation: inline

conserved
type: SequenceAnnotation
persistent identity: annotation1944488
display id: annotation1944488
version: 1
top level: U097NUL+D0
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_I732407/annotation1944488/range1944488/1

https://synbiohub.org/public/igem/BBa_I732407/annotation1944488/range1944488/1
type: Range
persistent identity: range1944488
display id: range1944488
version: 1
direction: direction/forward
top level: U097NUL+D0
start: 292
end: 295
orientation: inline

OmpR
type: ComponentDefinition
persistent identity: BBa R0083
display id: BBa_R0083
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter (OmpR, positive)
creator: Stephen Lee, Roshan Kumar, Joe Levine, Ziyan Chu (Polkadorks, IAP 2004)
created: 2004-01-27T12:00:00Z
modified: 2015-05-08T01:14:15Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Positively regulated, OmpR-controlled promoter. This promoter is derived from the upstream region of ompC. Phosphorylated OmpR binds to the operator site and activates transcription. This is a truncated version of BBa_R0082.
mutable notes: The promoter deletes the C2 and C3 regions of BBa_R0082 (bases 37-74 in BBa_R0082) and inserts an 8-base linker before the -35 and -10 signal region. The efficacy of this promoter versus the full ompC upstream region (BBa_R0082) or the ompF upstream region (BBa_R0084) is currently unknown. The a at base 41 is mutated to t to avoid XbaI site. Speculation that this was a previous assembly site.
mutable provenance: NC_000193 E. coli K12
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -10
sequence annotation: linker
sequence annotation: -35
sequence annotation: C1 OmpR
sequence annotation: A
sequence: https://synbiohub.org/public/igem/BBa_R0083_sequence/1

-10
type: SequenceAnnotation
persistent identity: annotation301226
display id: annotation301226
version: 1
top level: OmpR
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0083/annotation301226/range301226/1

https://synbiohub.org/public/igem/BBa_R0083/annotation301226/range301226/1
type: Range
persistent identity: range301226
display id: range301226
version: 1
direction: direction/forward
top level: OmpR
start: 68
end: 73
orientation: inline

linker
type: SequenceAnnotation
persistent identity: annotation301223
display id: annotation301223
version: 1
top level: OmpR
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_R0083/annotation301223/range301223/1

https://synbiohub.org/public/igem/BBa_R0083/annotation301223/range301223/1
type: Range
persistent identity: range301223
display id: range301223
version: 1
direction: direction/forward
top level: OmpR
start: 37
end: 44
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation301224
display id: annotation301224
version: 1
top level: OmpR
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0083/annotation301224/range301224/1

https://synbiohub.org/public/igem/BBa_R0083/annotation301224/range301224/1
type: Range
persistent identity: range301224
display id: range301224
version: 1
direction: direction/forward
top level: OmpR
start: 45
end: 50
orientation: inline

C1 OmpR
type: SequenceAnnotation
persistent identity: annotation301221
display id: annotation301221
version: 1
top level: OmpR
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0083/annotation301221/range301221/1

https://synbiohub.org/public/igem/BBa_R0083/annotation301221/range301221/1
type: Range
persistent identity: range301221
display id: range301221
version: 1
direction: direction/forward
top level: OmpR
start: 13
end: 30
orientation: inline

A
type: SequenceAnnotation
persistent identity: annotation306881
display id: annotation306881
version: 1
top level: OmpR
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_R0083/annotation306881/range306881/1

https://synbiohub.org/public/igem/BBa_R0083/annotation306881/range306881/1
type: Range
persistent identity: range306881
display id: range306881
version: 1
direction: direction/forward
top level: OmpR
start: 41
end: 41
orientation: inline

U061O11+D0
type: ComponentDefinition
persistent identity: BBa I732432
display id: BBa_I732432
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member (U061O11+D014O22)
creator: Zhan Jian
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732432_sequence/1

U061O11+D0
type: ComponentDefinition
persistent identity: BBa I732435
display id: BBa_I732435
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member (U061O11+D050O22)
creator: Zhan Jian
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732435_sequence/1

BBa_K292002
type: ComponentDefinition
persistent identity: BBa K292002
display id: BBa_K292002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pLac (LacI regulated) + Strong RBS
creator: David Charoy
created: 2009-10-18T11:00:00Z
modified: 2015-05-08T01:11:49Z
discontinued: false
dominant: false
group u list: _394_
m user id: 0
owner id: 5212
owning group id: 9
sample status: In stock
sample status: It's complicated
status: status/Available
status: status/Planning
bookmark: false
mutable description: This part contains the pLac promoter and a ribosome binding site (BBa_R0010 + BBa_B0030). The Lac promoter is constitutively activated, but it can be repressed by the Lac I repressor from E.coli. This promoter is really useful to control a gene expression by inactivating it with LacI repressor. When LacI is absent the gene should be expressed, and in the presence of LacI, pLac is inhibited. So this part is really suitable for gene expression control by inactivation of pLac with LacI. We can use this part to control a failed feedback mechanism which induces the Lambda phage lytic cycle. In E.Coli, pLac is a part of the lactose operon. LacI is constantly expressed and represses the pLac. In the presence of Lactose, LacI is inhibited and pLac is active and allows the transcription of beta-galactosidase. Lactose can be substituted by IPTG (Isopropyl β-D-1-thiogalactopyranoside). This part already contains a strong RBS to begin directly a gene transcription.
mutable notes: N/A
mutable provenance: Part:BBa_R0010 Part:BBa_B0030, Designed by Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. Group: NCBS, Prather Lab.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_B0030
component: BBa_R0010
sequence annotation: BBa_B0030
sequence annotation: BBa_R0010
sequence: https://synbiohub.org/public/igem/BBa_K292002_sequence/1

BBa_B0030
type: Component
persistent identity: component2054553
display id: component2054553
version: 1
top level: BBa_K292002
access: public
definition: https://synbiohub.org/public/igem/BBa_B0030/1

BBa_R0010
type: Component
persistent identity: component2054545
display id: component2054545
version: 1
top level: BBa_K292002
access: public
definition: LacI

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation2054553
display id: annotation2054553
version: 1
top level: BBa_K292002
location: https://synbiohub.org/public/igem/BBa_K292002/annotation2054553/range2054553/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_K292002/annotation2054553/range2054553/1
type: Range
persistent identity: range2054553
display id: range2054553
version: 1
direction: direction/forward
top level: BBa_K292002
start: 209
end: 223
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation2054545
display id: annotation2054545
version: 1
top level: BBa_K292002
location: https://synbiohub.org/public/igem/BBa_K292002/annotation2054545/range2054545/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_K292002/annotation2054545/range2054545/1
type: Range
persistent identity: range2054545
display id: range2054545
version: 1
direction: direction/forward
top level: BBa_K292002
start: 1
end: 200
orientation: inline

BBa_K180019
type: ComponentDefinition
persistent identity: BBa K180019
display id: BBa_K180019
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Scissors - Primary plasmid (part 2) [tac pR, mBanana and LuxI generator]
creator: Richard Wilson, Stephanie Chambers
created: 2009-10-18T11:00:00Z
modified: 2015-05-08T01:11:06Z
discontinued: false
dominant: false
experience: experience/None
group u list: _278_
m user id: 0
owner id: 4082
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Part of the iGEM2009 Team:Southampton rock-paper-scissors 'scissors' primary plasmid.
mutable notes: Regulated expression of LasI (C0178) and mBanana (K180009) by tac pR (K180000), controlled by presence of lac/IPTG or trp in the cell.
mutable provenance: See relevant basic biobrick parts (K180000, B0034, K180009, B0015).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K180000
component: BBa_B0034
component: BBa_B0010
component: BBa_K180009
component: BBa_B0012
component: BBa_C0161
sequence annotation: BBa_B0034
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_K180000
sequence annotation: BBa_C0161
sequence annotation: BBa_K180009
sequence: https://synbiohub.org/public/igem/BBa_K180019_sequence/1

BBa_K180000
type: Component
persistent identity: component2058401
display id: component2058401
version: 1
top level: BBa_K180019
access: public
definition: BBa_K180000

BBa_B0034
type: Component
persistent identity: component2058403
display id: component2058403
version: 1
top level: BBa_K180019
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0010
type: Component
persistent identity: component2058410
display id: component2058410
version: 1
top level: BBa_K180019
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_K180009
type: Component
persistent identity: component2058405
display id: component2058405
version: 1
top level: BBa_K180019
access: public
definition: https://synbiohub.org/public/igem/BBa_K180009/1

BBa_B0012
type: Component
persistent identity: component2058412
display id: component2058412
version: 1
top level: BBa_K180019
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_C0161
type: Component
persistent identity: component2058409
display id: component2058409
version: 1
top level: BBa_K180019
access: public
definition: https://synbiohub.org/public/igem/BBa_C0161/1

BBa_B0034
type: Component
persistent identity: component2058407
display id: component2058407
version: 1
top level: BBa_K180019
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2058403
display id: annotation2058403
version: 1
top level: BBa_K180019
location: https://synbiohub.org/public/igem/BBa_K180019/annotation2058403/range2058403/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K180019/annotation2058403/range2058403/1
type: Range
persistent identity: range2058403
display id: range2058403
version: 1
direction: direction/reverse
top level: BBa_K180019
start: 1456
end: 1467
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2058410
display id: annotation2058410
version: 1
top level: BBa_K180019
location: https://synbiohub.org/public/igem/BBa_K180019/annotation2058410/range2058410/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K180019/annotation2058410/range2058410/1
type: Range
persistent identity: range2058410
display id: range2058410
version: 1
direction: direction/forward
top level: BBa_K180019
start: 2801
end: 2880
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2058412
display id: annotation2058412
version: 1
top level: BBa_K180019
location: https://synbiohub.org/public/igem/BBa_K180019/annotation2058412/range2058412/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K180019/annotation2058412/range2058412/1
type: Range
persistent identity: range2058412
display id: range2058412
version: 1
direction: direction/forward
top level: BBa_K180019
start: 2889
end: 2929
orientation: inline

BBa_K180000
type: SequenceAnnotation
persistent identity: annotation2058401
display id: annotation2058401
version: 1
top level: BBa_K180019
location: https://synbiohub.org/public/igem/BBa_K180019/annotation2058401/range2058401/1
component: BBa_K180000

https://synbiohub.org/public/igem/BBa_K180019/annotation2058401/range2058401/1
type: Range
persistent identity: range2058401
display id: range2058401
version: 1
direction: direction/reverse
top level: BBa_K180019
start: 1
end: 1447
orientation: inline

BBa_C0161
type: SequenceAnnotation
persistent identity: annotation2058409
display id: annotation2058409
version: 1
top level: BBa_K180019
location: https://synbiohub.org/public/igem/BBa_K180019/annotation2058409/range2058409/1
component: BBa_C0161

https://synbiohub.org/public/igem/BBa_K180019/annotation2058409/range2058409/1
type: Range
persistent identity: range2058409
display id: range2058409
version: 1
direction: direction/reverse
top level: BBa_K180019
start: 2208
end: 2792
orientation: inline

BBa_K180009
type: SequenceAnnotation
persistent identity: annotation2058405
display id: annotation2058405
version: 1
top level: BBa_K180019
location: https://synbiohub.org/public/igem/BBa_K180019/annotation2058405/range2058405/1
component: BBa_K180009

https://synbiohub.org/public/igem/BBa_K180019/annotation2058405/range2058405/1
type: Range
persistent identity: range2058405
display id: range2058405
version: 1
direction: direction/reverse
top level: BBa_K180019
start: 1474
end: 2181
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2058407
display id: annotation2058407
version: 1
top level: BBa_K180019
location: https://synbiohub.org/public/igem/BBa_K180019/annotation2058407/range2058407/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K180019/annotation2058407/range2058407/1
type: Range
persistent identity: range2058407
display id: range2058407
version: 1
direction: direction/reverse
top level: BBa_K180019
start: 2190
end: 2201
orientation: inline

U097O11+D0
type: ComponentDefinition
persistent identity: BBa I732401
display id: BBa_I732401
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member (U097O11+D062NUL)
creator: Zhan Jian
created: 2007-10-02T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1592
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Our first NAND logical gate
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732401_sequence/1

BBa_I741020
type: ComponentDefinition
persistent identity: BBa I741020
display id: BBa_I741020
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: promoter to xylF without CRP and several binding sites for xylR
creator: Lucien Weiss
created: 2007-06-20T11:00:00Z
modified: 2015-08-31T04:08:02Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _143_
m user id: 0
owner id: 1763
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: removed RBS crp binding and xylR binding sites
mutable provenance: ecoli k12 chromosome
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: XylR-Xylose transcriptional activator activates transcription
sequence annotation: Transcription start base
sequence annotation: xylFp
sequence: https://synbiohub.org/public/igem/BBa_I741020_sequence/1

XylR-Xylose transcriptional activator activates transcription
type: SequenceAnnotation
persistent identity: annotation1935180
display id: annotation1935180
version: 1
top level: BBa_I741020
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I741020/annotation1935180/range1935180/1

https://synbiohub.org/public/igem/BBa_I741020/annotation1935180/range1935180/1
type: Range
persistent identity: range1935180
display id: range1935180
version: 1
direction: direction/forward
top level: BBa_I741020
start: 95
end: 112
orientation: inline

XylR-Xylose transcriptional activator activates transcription
type: SequenceAnnotation
persistent identity: annotation1935182
display id: annotation1935182
version: 1
top level: BBa_I741020
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I741020/annotation1935182/range1935182/1

https://synbiohub.org/public/igem/BBa_I741020/annotation1935182/range1935182/1
type: Range
persistent identity: range1935182
display id: range1935182
version: 1
direction: direction/forward
top level: BBa_I741020
start: 116
end: 132
orientation: inline

Transcription start base
type: SequenceAnnotation
persistent identity: annotation1935183
display id: annotation1935183
version: 1
top level: BBa_I741020
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I741020/annotation1935183/range1935183/1

https://synbiohub.org/public/igem/BBa_I741020/annotation1935183/range1935183/1
type: Range
persistent identity: range1935183
display id: range1935183
version: 1
direction: direction/forward
top level: BBa_I741020
start: 165
end: 165
orientation: inline

xylFp
type: SequenceAnnotation
persistent identity: annotation1935181
display id: annotation1935181
version: 1
top level: BBa_I741020
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I741020/annotation1935181/range1935181/1

https://synbiohub.org/public/igem/BBa_I741020/annotation1935181/range1935181/1
type: Range
persistent identity: range1935181
display id: range1935181
version: 1
direction: direction/forward
top level: BBa_I741020
start: 105
end: 185
orientation: inline

BBa_K091104
type: ComponentDefinition
persistent identity: BBa K091104
display id: BBa_K091104
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pLac/Mnt Hybrid Promoter
creator: Robert Cool
created: 2008-06-18T11:00:00Z
modified: 2015-05-08T01:08:37Z
discontinued: false
dominant: false
experience: experience/None
group u list: _191_
m user id: 0
owner id: 3080
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This promoter is a modified version of the Mnt promoter that is also responsive to LacI. The promoter should be repressed by Mnt repressor. It should also be repressed by LacI, and in the absence of Mnt repressor, should be induced by IPTG.
mutable notes: Mnt repressor binds as a tetramer to two half-operator sites. Introduction of a LacI binding site between the Mnt promoter -10 sequence and the start site for transcription should allow for repression by LacI. Accordingly, the hybrid promoter was designed by 1) Remove all bases of mnt promoter 3??? to -10: gagtcgtattaattt will be replaced by tgtgtggaattgtga, and 2) Position lacI binding site (composed of an inverted repeat) from the lacI regulated promoter (R0010) immediately 3??? to truncated mnt promoter.
mutable provenance: This part comes from the Mnt promoter and the LacI promoter
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: LacI
sequence annotation: Mnt
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_K091104_sequence/1

LacI
type: SequenceAnnotation
persistent identity: annotation1963738
display id: annotation1963738
version: 1
top level: BBa_K091104
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K091104/annotation1963738/range1963738/1

https://synbiohub.org/public/igem/BBa_K091104/annotation1963738/range1963738/1
type: Range
persistent identity: range1963738
display id: range1963738
version: 1
direction: direction/forward
top level: BBa_K091104
start: 53
end: 87
orientation: inline

Mnt
type: SequenceAnnotation
persistent identity: annotation1963743
display id: annotation1963743
version: 1
top level: BBa_K091104
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K091104/annotation1963743/range1963743/1

https://synbiohub.org/public/igem/BBa_K091104/annotation1963743/range1963743/1
type: Range
persistent identity: range1963743
display id: range1963743
version: 1
direction: direction/forward
top level: BBa_K091104
start: 1
end: 52
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1963737
display id: annotation1963737
version: 1
top level: BBa_K091104
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K091104/annotation1963737/range1963737/1

https://synbiohub.org/public/igem/BBa_K091104/annotation1963737/range1963737/1
type: Range
persistent identity: range1963737
display id: range1963737
version: 1
direction: direction/forward
top level: BBa_K091104
start: 47
end: 52
orientation: inline

BBa_K136004
type: ComponentDefinition
persistent identity: BBa K136004
display id: BBa_K136004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter of flhDC gene from E. coli flagella
creator: Cyprien Maisonnier
created: 2008-08-11T11:00:00Z
modified: 2015-05-08T01:10:02Z
discontinued: true
dominant: false
experience: experience/None
group u list: _211_
m user id: 0
owner id: 3012
owning group id: 9
sample status: Discontinued
status: status/Deleted
bookmark: false
mutable description: ''flhDC'' is the only class 1 gene that is part of the construction of the flagella. It is the master regulator of the biosynthesis of the flagella ( ''Ordering Genes in a Flagella Pathway by Analysis of Expression Kinetics from Living Bacteria'' - S. Kalir, J. McClure, K. Pabbaraju, C. Southward, M. Ronen, S. Leibler, M. G. Surette, U. Alon ). The promoter of ''flhDC'' is regulated by many transcription factors, for instance, OmpR* and EnvZ* are two repressors of this gene. '''Work in progress :''' Characterization of the repression by OmpR* and EnvZ*
mutable notes: The PCR on colony to amplify the promoter of ''flhDC'' did not work. We amplified both the promoter and the gene and then we did a specific amplification of the promoter from the full operon. We had a PCR amplicon of the right length this time. The primers used to amplify the promoter and the gene are : * Forward : GTTTCTTCGAATTCGCGGCCGCTTCTAGAGTTGTATGTGCGTGTAGTGACGAGTACAG * Reverse : GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTAAACAGCCTGTACTCTCTGTTCATCC Then to amplify only the promoter we used : * Forward : GTTTCTTCGAATTCGCGGCCGCTTCTAGAGTTGTATGTGCGTGTAGTGACGAGTACAG * Reverse : GTTTCTTCCTGCAGCGGCCGCTACTAGTATCCCACCCAGAATAACCAACTTTAT
mutable provenance: This part comes from the genome of ''E.coli'' K12 strain MG1655.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: OmpR binding site
sequence annotation: Transcription start base
sequence: https://synbiohub.org/public/igem/BBa_K136004_sequence/1

OmpR binding site
type: SequenceAnnotation
persistent identity: annotation1971578
display id: annotation1971578
version: 1
top level: BBa_K136004
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K136004/annotation1971578/range1971578/1

https://synbiohub.org/public/igem/BBa_K136004/annotation1971578/range1971578/1
type: Range
persistent identity: range1971578
display id: range1971578
version: 1
direction: direction/forward
top level: BBa_K136004
start: 43
end: 63
orientation: inline

Transcription start base
type: SequenceAnnotation
persistent identity: annotation1971577
display id: annotation1971577
version: 1
top level: BBa_K136004
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K136004/annotation1971577/range1971577/1

https://synbiohub.org/public/igem/BBa_K136004/annotation1971577/range1971577/1
type: Range
persistent identity: range1971577
display id: range1971577
version: 1
direction: direction/forward
top level: BBa_K136004
start: 36
end: 36
orientation: inline

U049O11+D0
type: ComponentDefinition
persistent identity: BBa I732439
display id: BBa_I732439
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member (U049O11+D026O22)
creator: Zhan Jian
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732439_sequence/1

BBa_K136002
type: ComponentDefinition
persistent identity: BBa K136002
display id: BBa_K136002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter of flgB gene from E. coli flagella
creator: Cyprien Maisonnier
created: 2008-08-11T11:00:00Z
modified: 2015-05-08T01:10:02Z
discontinued: true
dominant: false
experience: experience/None
group u list: _211_
m user id: 0
owner id: 3012
owning group id: 9
sample status: Discontinued
status: status/Deleted
bookmark: false
mutable description: ''flgB'' is a class 2 gene that is part of the construction of the flagella ( ''Ordering Genes in a Flagella Pathway by Analysis of Expression Kinetics from Living Bacteria'' - S. Kalir, J. McClure, K. Pabbaraju, C. Southward, M. Ronen, S. Leibler, M. G. Surette, U. Alon ). Its promoter is consitutively repressed. It is activated by FlhD4C2 and maybe by FliA with different strength ( ''Using a Quantitative Blueprint to Reprogram the Dynamics of the Flagella Gene Network'' - Shiraz Kalir and Uri Alon ). '''Work in progress :''' Deeper quantitative characterization
mutable notes: The primers used to amplify this sequence are : *Forward : GTTTCTTCGAATTCGCGGCCGCTTCTAGAGACAGTATCGCGATGATCGCCACGCTACG *Reverse : GTTTCTTCCTGCAGCGGCCGCTACTAGTAAGCATATCTCCTCCGCAGGTATCAAAATT
mutable provenance: This part comes from the genome of ''E.coli'' K12 strain MG1655.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: Initiation of Transcription
sequence annotation: FlhDC Binding site 2
sequence annotation: FlhDC Binding site 1
sequence: https://synbiohub.org/public/igem/BBa_K136002_sequence/1

Initiation of Transcription
type: SequenceAnnotation
persistent identity: annotation1971573
display id: annotation1971573
version: 1
top level: BBa_K136002
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K136002/annotation1971573/range1971573/1

https://synbiohub.org/public/igem/BBa_K136002/annotation1971573/range1971573/1
type: Range
persistent identity: range1971573
display id: range1971573
version: 1
direction: direction/forward
top level: BBa_K136002
start: 173
end: 173
orientation: inline

FlhDC Binding site 2
type: SequenceAnnotation
persistent identity: annotation1971572
display id: annotation1971572
version: 1
top level: BBa_K136002
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K136002/annotation1971572/range1971572/1

https://synbiohub.org/public/igem/BBa_K136002/annotation1971572/range1971572/1
type: Range
persistent identity: range1971572
display id: range1971572
version: 1
direction: direction/forward
top level: BBa_K136002
start: 124
end: 139
orientation: inline

FlhDC Binding site 1
type: SequenceAnnotation
persistent identity: annotation1971571
display id: annotation1971571
version: 1
top level: BBa_K136002
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K136002/annotation1971571/range1971571/1

https://synbiohub.org/public/igem/BBa_K136002/annotation1971571/range1971571/1
type: Range
persistent identity: range1971571
display id: range1971571
version: 1
direction: direction/forward
top level: BBa_K136002
start: 98
end: 113
orientation: inline

BBa_I765007
type: ComponentDefinition
persistent identity: BBa I765007
display id: BBa_I765007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Fe and UV promoters
creator: Claudia Guevara and Alex Quintero
created: 2007-10-25T11:00:00Z
modified: 2015-08-31T04:08:09Z
discontinued: false
dominant: false
experience: experience/None
group u list: _118_
m user id: 0
owner id: 2267
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Regulated by the presence of Iron and UV light irradiation
mutable notes: None
mutable provenance: Genomic sequence
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
component: BBa_I765001
component: BBa_I765000
sequence annotation: BBa_I765000
sequence annotation: BBa_I765001
sequence: https://synbiohub.org/public/igem/BBa_I765007_sequence/1

BBa_I765001
type: Component
persistent identity: component1958276
display id: component1958276
version: 1
top level: BBa_I765007
access: public
definition: pUV

BBa_I765000
type: Component
persistent identity: component1958274
display id: component1958274
version: 1
top level: BBa_I765007
access: public
definition: https://synbiohub.org/public/igem/BBa_I765000/1

BBa_I765000
type: SequenceAnnotation
persistent identity: annotation1958274
display id: annotation1958274
version: 1
top level: BBa_I765007
location: https://synbiohub.org/public/igem/BBa_I765007/annotation1958274/range1958274/1
component: BBa_I765000

https://synbiohub.org/public/igem/BBa_I765007/annotation1958274/range1958274/1
type: Range
persistent identity: range1958274
display id: range1958274
version: 1
direction: direction/reverse
top level: BBa_I765007
start: 1
end: 1044
orientation: inline

BBa_I765001
type: SequenceAnnotation
persistent identity: annotation1958276
display id: annotation1958276
version: 1
top level: BBa_I765007
location: https://synbiohub.org/public/igem/BBa_I765007/annotation1958276/range1958276/1
component: BBa_I765001

https://synbiohub.org/public/igem/BBa_I765007/annotation1958276/range1958276/1
type: Range
persistent identity: range1958276
display id: range1958276
version: 1
direction: direction/reverse
top level: BBa_I765007
start: 1053
end: 1128
orientation: inline

BBa_M13104
type: ComponentDefinition
persistent identity: BBa M13104
display id: BBa_M13104
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: M13K07 gene IV promoter
creator: Natalie Kuldell
created: 2006-12-15T12:00:00Z
modified: 2015-05-08T01:13:55Z
discontinued: false
dominant: false
group u list: _45_
m user id: 0
owner id: 314
owning group id: 1
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This part is from the bacteriophage M13 genome, bp 4055-4103 in M13K07. It directs transcription of M13 gene IV (BBa_M13504, BBa_M13004). Its identity as a promoter was described in Gene (1980) 11:129-148 based on -10,-35 homology and fragment of genome able to bind RNAP.
mutable notes: none
mutable provenance: New England Biolabs M13K07. Sequenced in 2002 by L. Panganaban.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_M13104_sequence/1

BBa_K823005
type: ComponentDefinition
persistent identity: BBa K823005
display id: BBa_K823005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Anderson promoter J23101
creator: Korinna Kraft
created: 2012-09-07T11:00:00Z
modified: 2015-07-07T11:44:25Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1081_
m user id: 4206
owner id: 12081
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Anderson promoter J23101 without RFP in backbone vector pSB1C3 to easily fuse the promoter with other reporters e.g. the lux operon.
mutable notes: no considerations
mutable provenance: Partsregistry
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -35 box
sequence annotation: -10 box
sequence annotation: J23101
sequence: https://synbiohub.org/public/igem/BBa_K823005_sequence/1

-35 box
type: SequenceAnnotation
persistent identity: annotation2182510
display id: annotation2182510
version: 1
top level: BBa_K823005
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K823005/annotation2182510/range2182510/1

https://synbiohub.org/public/igem/BBa_K823005/annotation2182510/range2182510/1
type: Range
persistent identity: range2182510
display id: range2182510
version: 1
direction: direction/forward
top level: BBa_K823005
start: 1
end: 6
orientation: inline

-10 box
type: SequenceAnnotation
persistent identity: annotation2182511
display id: annotation2182511
version: 1
top level: BBa_K823005
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K823005/annotation2182511/range2182511/1

https://synbiohub.org/public/igem/BBa_K823005/annotation2182511/range2182511/1
type: Range
persistent identity: range2182511
display id: range2182511
version: 1
direction: direction/forward
top level: BBa_K823005
start: 24
end: 29
orientation: inline

J23101
type: SequenceAnnotation
persistent identity: annotation2182509
display id: annotation2182509
version: 1
top level: BBa_K823005
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K823005/annotation2182509/range2182509/1

https://synbiohub.org/public/igem/BBa_K823005/annotation2182509/range2182509/1
type: Range
persistent identity: range2182509
display id: range2182509
version: 1
direction: direction/forward
top level: BBa_K823005
start: 1
end: 35
orientation: inline

BBa_K136003
type: ComponentDefinition
persistent identity: BBa K136003
display id: BBa_K136003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter of flhB gene from E. coli flagella
creator: Cyprien Maisonnier
created: 2008-08-11T11:00:00Z
modified: 2015-05-08T01:10:02Z
discontinued: true
dominant: false
experience: experience/None
group u list: _211_
m user id: 0
owner id: 3012
owning group id: 9
sample status: Discontinued
status: status/Deleted
bookmark: false
mutable description: ''flhB'' is a class 2 gene that is part of the construction of the flagella ( ''Ordering Genes in a Flagella Pathway by Analysis of Expression Kinetics from Living Bacteria'' - S. Kalir, J. McClure, K. Pabbaraju, C. Southward, M. Ronen, S. Leibler, M. G. Surette, U. Alon ). Its promoter is consitutively repressed. It is activated by FlhD4C2 and maybe by FliA with different strength ( ''Using a Quantitative Blueprint to Reprogram the Dynamics of the Flagella Gene Network'' - Shiraz Kalir and Uri Alon ). '''Work in progress :''' Deeper quantitative characterization
mutable notes: The primers used to amplify this sequence are : *Forward : GTTTCTTCGAATTCGCGGCCGCTTCTAGAGCCACGTCATATCAGGCGGTCTGATAAGG *Reverse : GTTTCTTCCTGCAGCGGCCGCTACTAGTAGTTTTGTCGTCGCTCTCGTCAGACACGTC
mutable provenance: This part comes from the genome of ''E.coli'' K12 strain MG1655.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: FlhDC Binding site 1
sequence annotation: Initiation for Transcription
sequence annotation: FlhDC Binding site 2
sequence: https://synbiohub.org/public/igem/BBa_K136003_sequence/1

FlhDC Binding site 1
type: SequenceAnnotation
persistent identity: annotation1971574
display id: annotation1971574
version: 1
top level: BBa_K136003
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K136003/annotation1971574/range1971574/1

https://synbiohub.org/public/igem/BBa_K136003/annotation1971574/range1971574/1
type: Range
persistent identity: range1971574
display id: range1971574
version: 1
direction: direction/forward
top level: BBa_K136003
start: 79
end: 94
orientation: inline

Initiation for Transcription
type: SequenceAnnotation
persistent identity: annotation1971576
display id: annotation1971576
version: 1
top level: BBa_K136003
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K136003/annotation1971576/range1971576/1

https://synbiohub.org/public/igem/BBa_K136003/annotation1971576/range1971576/1
type: Range
persistent identity: range1971576
display id: range1971576
version: 1
direction: direction/forward
top level: BBa_K136003
start: 153
end: 153
orientation: inline

FlhDC Binding site 2
type: SequenceAnnotation
persistent identity: annotation1971575
display id: annotation1971575
version: 1
top level: BBa_K136003
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K136003/annotation1971575/range1971575/1

https://synbiohub.org/public/igem/BBa_K136003/annotation1971575/range1971575/1
type: Range
persistent identity: range1971575
display id: range1971575
version: 1
direction: direction/forward
top level: BBa_K136003
start: 106
end: 121
orientation: inline

NOR1
type: ComponentDefinition
persistent identity: BBa I732351
display id: BBa_I732351
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: NOR Candidate (U037O11D002O22)
creator: Zhan Jian
created: 2007-10-02T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: true
experience: experience/Issues
group u list: _156_
m user id: 0
owner id: 1592
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: U97NUL D001O16
mutable notes: N/A
mutable provenance: LacIq
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732351_sequence/1

U097O11+D0
type: ComponentDefinition
persistent identity: BBa I732415
display id: BBa_I732415
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Family Member (U097O11+D026O22)
creator: Zhan Jian
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I732415_sequence/1

BBa_K112701
type: ComponentDefinition
persistent identity: BBa K112701
display id: BBa_K112701
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: hns promoter
creator: Bing Xia
created: 2008-10-20T11:00:00Z
modified: 2015-05-08T01:09:18Z
discontinued: false
dominant: false
group u list: _224_
m user id: 0
owner id: 2998
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Phns in BBb1 format. ---- This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at: [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
mutable provenance: Construction of [<Phns>] Basic Part K112701 PCR dv001/dv006 on Phns from E. coli K12 MG1655 (~342 bp, gp = A) PCR dv005/dv003 on Phns from E. coli K12 MG1655 (225 bp, gp = B) PCR dv004/dv002 on Phns from E. coli K12 MG1655 (~149 bp, gp = C) --------------------------------------------------- PCR dv001/dv003 on A+B (355 bp, gp = D) --------------------------------------------------- PCR dv001/dv002 on C+D (697bp, EcoRI/BamHI/DpnI) Sub into pBca1256 (EcoRI/BamHI, 2472+697) Product is pBca1256-K112701 ----------------------------------------------- dv001 Forward Biobricking of [<Phns>] CgATAgaattcATGagatctGAAATATAGCTGTGCCATCAGCC dv002 Reverse Biobricking of [<Phns>] cagtcggatccGCACGAAGAGTACGGATG dv003 Removing the 2nd EcoRI site in [<Phns>] (R) GCCAGGAATGTAAGGgATTCAAAATTGTTC dv004 Removing the 2ND EcoRI site in [<Phns>] (F) GAACAATTTTGAATcCCTTACATTCCTGGC dv005 Removing the FIRST EcoRI site in [<Phns>] (F)CGCTTAATAGGGgATTCTCGTAAACAC dv006 Removing the FIRST EcoRI site in [<Phns>] (R)GTGTTTACGAGAATcCCCTATTAAGCG
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K112701_sequence/1

BBa_K116401
type: ComponentDefinition
persistent identity: BBa K116401
display id: BBa_K116401
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: external phosphate sensing promoter
creator: Chih-Hsien Yang
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:09:32Z
discontinued: false
dominant: false
group u list: _210_
m user id: 0
owner id: 592
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: promoter of phoB in E.coli, an external phosphate regulated promoter which can be activated when encountering phosphate limitation.
mutable notes: The promoter region should contain PhoB binding site
mutable provenance: E. coli K12 genomic DNA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -35
sequence annotation: -10
sequence annotation: PhoB binding site
sequence: https://synbiohub.org/public/igem/BBa_K116401_sequence/1

-35
type: SequenceAnnotation
persistent identity: annotation1995352
display id: annotation1995352
version: 1
top level: BBa_K116401
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K116401/annotation1995352/range1995352/1

https://synbiohub.org/public/igem/BBa_K116401/annotation1995352/range1995352/1
type: Range
persistent identity: range1995352
display id: range1995352
version: 1
direction: direction/forward
top level: BBa_K116401
start: 421
end: 429
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1995353
display id: annotation1995353
version: 1
top level: BBa_K116401
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K116401/annotation1995353/range1995353/1

https://synbiohub.org/public/igem/BBa_K116401/annotation1995353/range1995353/1
type: Range
persistent identity: range1995353
display id: range1995353
version: 1
direction: direction/forward
top level: BBa_K116401
start: 448
end: 455
orientation: inline

PhoB binding site
type: SequenceAnnotation
persistent identity: annotation1995351
display id: annotation1995351
version: 1
top level: BBa_K116401
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K116401/annotation1995351/range1995351/1

https://synbiohub.org/public/igem/BBa_K116401/annotation1995351/range1995351/1
type: Range
persistent identity: range1995351
display id: range1995351
version: 1
direction: direction/forward
top level: BBa_K116401
start: 420
end: 439
orientation: inline

BBa_J23101
type: ComponentDefinition
persistent identity: BBa J23101
display id: BBa_J23101
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: constitutive promoter family member
creator: John Anderson
created: 2006-08-03T11:00:00Z
modified: 2015-08-31T04:08:40Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: later
mutable notes: N/A
mutable provenance: later
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J23101_sequence/1

Pu
type: ComponentDefinition
persistent identity: BBa I723020
display id: BBa_I723020
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Pu
creator: Scott Ramsay
created: 2007-10-24T11:00:00Z
modified: 2015-08-31T04:07:54Z
discontinued: false
dominant: false
experience: experience/None
group u list: _126_
m user id: 0
owner id: 2140
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This is the promoter activated by the transcriptional regulator XylR (BBa_I723136) in response to detection of environmental xylene.
mutable notes: [Christine/Maia]
mutable provenance: [Christine/Maia]
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: Pu
sequence: https://synbiohub.org/public/igem/BBa_I723020_sequence/1

Pu
type: SequenceAnnotation
persistent identity: annotation1955192
display id: annotation1955192
version: 1
top level: Pu
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I723020/annotation1955192/range1955192/1

https://synbiohub.org/public/igem/BBa_I723020/annotation1955192/range1955192/1
type: Range
persistent identity: range1955192
display id: range1955192
version: 1
direction: direction/forward
top level: Pu
start: 1
end: 320
orientation: inline

CP1LacPI1
type: ComponentDefinition
persistent identity: BBa K418000
display id: BBa_K418000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: IPTG inducible Lac promoter cassette
creator: Northwestern University iGEM 2010
created: 2010-10-11T11:00:00Z
modified: 2015-05-08T01:12:28Z
discontinued: false
dominant: true
experience: experience/None
group u list: _532_
m user id: 0
owner id: 6657
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: constituitive promoter + LacPI
mutable notes: blah
mutable provenance: From assembly of kit parts.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_J23100
component: BBa_Q01121
sequence annotation: BBa_J23100
sequence annotation: BBa_Q01121
sequence: https://synbiohub.org/public/igem/BBa_K418000_sequence/1

BBa_J23100
type: Component
persistent identity: component2263723
display id: component2263723
version: 1
top level: CP1LacPI1
access: public
definition: BBa_J23100

BBa_Q01121
type: Component
persistent identity: component2263742
display id: component2263742
version: 1
top level: CP1LacPI1
access: public
definition: https://synbiohub.org/public/igem/BBa_Q01121/1

BBa_J23100
type: SequenceAnnotation
persistent identity: annotation2263723
display id: annotation2263723
version: 1
top level: CP1LacPI1
location: https://synbiohub.org/public/igem/BBa_K418000/annotation2263723/range2263723/1
component: BBa_J23100

https://synbiohub.org/public/igem/BBa_K418000/annotation2263723/range2263723/1
type: Range
persistent identity: range2263723
display id: range2263723
version: 1
direction: direction/reverse
top level: CP1LacPI1
start: 1
end: 35
orientation: inline

BBa_Q01121
type: SequenceAnnotation
persistent identity: annotation2263742
display id: annotation2263742
version: 1
top level: CP1LacPI1
location: https://synbiohub.org/public/igem/BBa_K418000/annotation2263742/range2263742/1
component: BBa_Q01121

https://synbiohub.org/public/igem/BBa_K418000/annotation2263742/range2263742/1
type: Range
persistent identity: range2263742
display id: range2263742
version: 1
direction: direction/reverse
top level: CP1LacPI1
start: 44
end: 1416
orientation: inline

BBa_J16105
type: ComponentDefinition
persistent identity: BBa J16105
display id: BBa_J16105
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: HelPp - "Help" Dependant promoter
creator: James Brown
created: 2006-07-23T11:00:00Z
modified: 2015-08-31T04:08:33Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 740
owning group id: 1
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Transciptional regulator reliant on screams of someone, somewhere in trouble.
mutable notes: Very, Very sensitive
mutable provenance: DNA sample - Taken from Clark Kent's Coffee Mug at the Daily Planet
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J16105_sequence/1

lux pR
type: ComponentDefinition
persistent identity: BBa R1062
display id: BBa_R1062
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter, Standard (luxR and HSL regulated -- lux pR)
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr Drew Endy
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:15Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: [Note: This is the same part as R0062 except that the -10 and -35 sites and spacing have been changed to comply with BBa_S0001]. Promoter activated by LuxR in concert with HSL The lux cassette of V. fischeri contains a left and a right promoter. The right promoter gives weak constitutive expression of downstream genes.This expression is up-regulated by the action of the LuxR activator protein complexed with the autoinducer, 3-oxo-hexanoyl-HSL. Two molecules of LuxR protein form a complex with two molecules of the signalling compound homoserine lactone (HSL). This complex binds to a palindromic site on the promoter, increasing the rate of transcription.
mutable notes: This promoter is based on the Vibrio fischeri quorum sensing gene promoters. Two genes LuxI and LuxR and transcribed in opposite directions as shown below. The original sequence from which the parts <bb_part>BBa_R0062</bb_part> and <bb_part>BBa_R0063</bb_part> were derived is shown in the picture below. <img src="<bb_file part=BBa_R0062>Image1.gif</bb_file>" width="614" height="362"> Modified to comply with BBa_S0001: TTGACA-17N-GATACT
mutable provenance: V. fischeri
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: start
sequence annotation: LuxR/HSL
sequence annotation: BBa_R1062
sequence annotation: -35
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R1062_sequence/1

start
type: SequenceAnnotation
persistent identity: annotation2100
display id: annotation2100
version: 1
top level: lux pR
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_R1062/annotation2100/range2100/1

https://synbiohub.org/public/igem/BBa_R1062/annotation2100/range2100/1
type: Range
persistent identity: range2100
display id: range2100
version: 1
direction: direction/forward
top level: lux pR
start: 54
end: 54
orientation: inline

LuxR/HSL
type: SequenceAnnotation
persistent identity: annotation2098
display id: annotation2098
version: 1
top level: lux pR
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R1062/annotation2098/range2098/1

https://synbiohub.org/public/igem/BBa_R1062/annotation2098/range2098/1
type: Range
persistent identity: range2098
display id: range2098
version: 1
direction: direction/forward
top level: lux pR
start: 1
end: 20
orientation: inline

BBa_R1062
type: SequenceAnnotation
persistent identity: annotation7077
display id: annotation7077
version: 1
top level: lux pR
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_R1062/annotation7077/range7077/1

https://synbiohub.org/public/igem/BBa_R1062/annotation7077/range7077/1
type: Range
persistent identity: range7077
display id: range7077
version: 1
direction: direction/forward
top level: lux pR
start: 1
end: 56
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2101
display id: annotation2101
version: 1
top level: lux pR
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R1062/annotation2101/range2101/1

https://synbiohub.org/public/igem/BBa_R1062/annotation2101/range2101/1
type: Range
persistent identity: range2101
display id: range2101
version: 1
direction: direction/forward
top level: lux pR
start: 20
end: 25
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2099
display id: annotation2099
version: 1
top level: lux pR
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R1062/annotation2099/range2099/1

https://synbiohub.org/public/igem/BBa_R1062/annotation2099/range2099/1
type: Range
persistent identity: range2099
display id: range2099
version: 1
direction: direction/forward
top level: lux pR
start: 42
end: 48
orientation: inline

BBa_I714889
type: ComponentDefinition
persistent identity: BBa I714889
display id: BBa_I714889
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: OR21 of PR and PRM
creator: Chunbo Lou
created: 2007-10-19T11:00:00Z
modified: 2015-08-31T04:07:48Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _142_
m user id: 0
owner id: 1911
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: we synthesize the OR promoter by replacing OR3 binding site of lambda CI with OR1 binding site of CI434.
mutable notes: making sure CI434 protein can repress CI's transcription.
mutable provenance: similar to lambda lysis/lysogenic regulatory region.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -10
sequence annotation: OR1_434
sequence annotation: -35
sequence: https://synbiohub.org/public/igem/BBa_I714889_sequence/1

-10
type: SequenceAnnotation
persistent identity: annotation1951424
display id: annotation1951424
version: 1
top level: BBa_I714889
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I714889/annotation1951424/range1951424/1

https://synbiohub.org/public/igem/BBa_I714889/annotation1951424/range1951424/1
type: Range
persistent identity: range1951424
display id: range1951424
version: 1
direction: direction/reverse
top level: BBa_I714889
start: 26
end: 31
orientation: inline

OR1_434
type: SequenceAnnotation
persistent identity: annotation1951383
display id: annotation1951383
version: 1
top level: BBa_I714889
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I714889/annotation1951383/range1951383/1

https://synbiohub.org/public/igem/BBa_I714889/annotation1951383/range1951383/1
type: Range
persistent identity: range1951383
display id: range1951383
version: 1
direction: direction/forward
top level: BBa_I714889
start: 1
end: 14
orientation: inline

OR1_434
type: SequenceAnnotation
persistent identity: annotation1951380
display id: annotation1951380
version: 1
top level: BBa_I714889
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I714889/annotation1951380/range1951380/1

https://synbiohub.org/public/igem/BBa_I714889/annotation1951380/range1951380/1
type: Range
persistent identity: range1951380
display id: range1951380
version: 1
direction: direction/forward
top level: BBa_I714889
start: 31
end: 44
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1951382
display id: annotation1951382
version: 1
top level: BBa_I714889
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I714889/annotation1951382/range1951382/1

https://synbiohub.org/public/igem/BBa_I714889/annotation1951382/range1951382/1
type: Range
persistent identity: range1951382
display id: range1951382
version: 1
direction: direction/reverse
top level: BBa_I714889
start: 49
end: 54
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1951425
display id: annotation1951425
version: 1
top level: BBa_I714889
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I714889/annotation1951425/range1951425/1

https://synbiohub.org/public/igem/BBa_I714889/annotation1951425/range1951425/1
type: Range
persistent identity: range1951425
display id: range1951425
version: 1
direction: direction/forward
top level: BBa_I714889
start: 67
end: 72
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1951381
display id: annotation1951381
version: 1
top level: BBa_I714889
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I714889/annotation1951381/range1951381/1

https://synbiohub.org/public/igem/BBa_I714889/annotation1951381/range1951381/1
type: Range
persistent identity: range1951381
display id: range1951381
version: 1
direction: direction/forward
top level: BBa_I714889
start: 89
end: 95
orientation: inline

BBa_I741018
type: ComponentDefinition
persistent identity: BBa I741018
display id: BBa_I741018
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Right facing promoter (for xylF) controlled by xylR and CRP-cAMP
creator: Lucien Weiss
created: 2007-06-20T11:00:00Z
modified: 2015-08-31T04:08:01Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _143_
m user id: 0
owner id: 1763
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: potential looping feature possibly removed
mutable notes: removed RBS, left promoter, and several xylR binding sites
mutable provenance: ecoli k12 chromosome
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: +1
sequence annotation: Promoter: xylFp
sequence annotation: CRP-cAMP duel regulator binding site
sequence annotation: xylR activator binding site
sequence: https://synbiohub.org/public/igem/BBa_I741018_sequence/1

+1
type: SequenceAnnotation
persistent identity: annotation1935202
display id: annotation1935202
version: 1
top level: BBa_I741018
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I741018/annotation1935202/range1935202/1

https://synbiohub.org/public/igem/BBa_I741018/annotation1935202/range1935202/1
type: Range
persistent identity: range1935202
display id: range1935202
version: 1
direction: direction/forward
top level: BBa_I741018
start: 195
end: 195
orientation: inline

Promoter: xylFp
type: SequenceAnnotation
persistent identity: annotation1935200
display id: annotation1935200
version: 1
top level: BBa_I741018
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I741018/annotation1935200/range1935200/1

https://synbiohub.org/public/igem/BBa_I741018/annotation1935200/range1935200/1
type: Range
persistent identity: range1935200
display id: range1935200
version: 1
direction: direction/forward
top level: BBa_I741018
start: 138
end: 215
orientation: inline

CRP-cAMP duel regulator binding site
type: SequenceAnnotation
persistent identity: annotation1935203
display id: annotation1935203
version: 1
top level: BBa_I741018
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I741018/annotation1935203/range1935203/1

https://synbiohub.org/public/igem/BBa_I741018/annotation1935203/range1935203/1
type: Range
persistent identity: range1935203
display id: range1935203
version: 1
direction: direction/forward
top level: BBa_I741018
start: 4
end: 25
orientation: inline

xylR activator binding site
type: SequenceAnnotation
persistent identity: annotation1935201
display id: annotation1935201
version: 1
top level: BBa_I741018
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I741018/annotation1935201/range1935201/1

https://synbiohub.org/public/igem/BBa_I741018/annotation1935201/range1935201/1
type: Range
persistent identity: range1935201
display id: range1935201
version: 1
direction: direction/forward
top level: BBa_I741018
start: 146
end: 162
orientation: inline

xylR activator binding site
type: SequenceAnnotation
persistent identity: annotation1935199
display id: annotation1935199
version: 1
top level: BBa_I741018
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I741018/annotation1935199/range1935199/1

https://synbiohub.org/public/igem/BBa_I741018/annotation1935199/range1935199/1
type: Range
persistent identity: range1935199
display id: range1935199
version: 1
direction: direction/forward
top level: BBa_I741018
start: 125
end: 142
orientation: inline

BBa_K658008
type: ComponentDefinition
persistent identity: BBa K658008
display id: BBa_K658008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: position 3&5 mutated promoter lux pR-3/5 (luxR & HSL regulated)
creator: Yidan Hu
created: 2011-09-30T11:00:00Z
modified: 2015-05-08T01:13:02Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _835_
m user id: 0
owner id: 10207
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: The position 3&5 mutated promoter lux pR-3/5 is a product of site-directed mutagenesis of promoter lux pR (BBa_R0062). The promoter lux pR is based on the Vibrio fischeri quorum sensing gene promoters. The position 5 mutated promoter lux pR-3/5 gives a lower expression of downstream genes in an autoinduction system. In another word, the lux pR-3/5 has a lower efficiency than the lux pR(BBa_R0062). Similar to promoter lux pR, the expression is up-regulated by the action of LuxR protein combined with the signal molecule HSL.
mutable notes: We have generated two point mutants by site-directed mutagenesis from the sequence of lux pR(BBa_R0062). In these mutants, the G residue of position 5 was changed to a C and the C residue of position 3 was changed to a T respectively.
mutable provenance: The promoter lux pR (BBa_R0062).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: LuxR/HSL
sequence annotation: K658008
sequence annotation: -35
sequence annotation: -10
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_K658008_sequence/1

LuxR/HSL
type: SequenceAnnotation
persistent identity: annotation2148176
display id: annotation2148176
version: 1
top level: BBa_K658008
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K658008/annotation2148176/range2148176/1

https://synbiohub.org/public/igem/BBa_K658008/annotation2148176/range2148176/1
type: Range
persistent identity: range2148176
display id: range2148176
version: 1
direction: direction/forward
top level: BBa_K658008
start: 1
end: 20
orientation: inline

K658008
type: SequenceAnnotation
persistent identity: annotation2148175
display id: annotation2148175
version: 1
top level: BBa_K658008
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K658008/annotation2148175/range2148175/1

https://synbiohub.org/public/igem/BBa_K658008/annotation2148175/range2148175/1
type: Range
persistent identity: range2148175
display id: range2148175
version: 1
direction: direction/forward
top level: BBa_K658008
start: 1
end: 55
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2148173
display id: annotation2148173
version: 1
top level: BBa_K658008
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K658008/annotation2148173/range2148173/1

https://synbiohub.org/public/igem/BBa_K658008/annotation2148173/range2148173/1
type: Range
persistent identity: range2148173
display id: range2148173
version: 1
direction: direction/forward
top level: BBa_K658008
start: 20
end: 25
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2148174
display id: annotation2148174
version: 1
top level: BBa_K658008
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K658008/annotation2148174/range2148174/1

https://synbiohub.org/public/igem/BBa_K658008/annotation2148174/range2148174/1
type: Range
persistent identity: range2148174
display id: range2148174
version: 1
direction: direction/forward
top level: BBa_K658008
start: 42
end: 47
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2148172
display id: annotation2148172
version: 1
top level: BBa_K658008
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K658008/annotation2148172/range2148172/1

https://synbiohub.org/public/igem/BBa_K658008/annotation2148172/range2148172/1
type: Range
persistent identity: range2148172
display id: range2148172
version: 1
direction: direction/forward
top level: BBa_K658008
start: 53
end: 53
orientation: inline