//rnap/eukaryote/yeast
type: Collection
persistent identity: rnap eukaryote yeast
display id: rnap_eukaryote_yeast
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
owned by: james
owned by: myers
member: BBa_K165000
member: BBa_K110016
member: BBa_K165030
member: BBa_I766214
member: BBa_K110007
member: BBa_K165035
member: BBa_K110009
member: BBa_K124002
member: BBa_I766200
member: BBa_I766556
member: BBa_K110004
member: BBa_M31201
member: BBa_K105031
member: BBa_I766557
member: P0310
member: BBa_K165041
member: BBa_K105030
member: BBa_K110005
member: pTEF
member: BBa_K124000
member: BBa_I766558
member: BBa_K165042
member: GAL1
member: BBa_K106699
member: BBa_K110006
member: BBa_K165038
member: BBa_K165043
member: BBa_K122000
member: BBa_K165039
member: BBa_K165031
member: BBa_K149001
member: BBa_K165001
member: BBa_K165015
member: BBa_K110008
member: BBa_K165036
member: BBa_K105027
member: BBa_K165032
member: BBa_K165040
member: BBa_K165016
member: BBa_K105028
member: BBa_K110014
member: BBa_K165033
member: BBa_J24813
member: BBa_K586000
member: BBa_I766555
member: BBa_K110015
member: BBa_K165034
member: BBa_K105029

BBa_K165001
type: ComponentDefinition
persistent identity: BBa K165001
display id: BBa_K165001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pGAL1+ w/XhoI sites
creator: John Szymanski
created: 2008-10-25T11:00:00Z
modified: 2015-05-08T01:10:55Z
discontinued: false
dominant: false
group u list: _267_
m user id: 0
owner id: 2512
owning group id: 58
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This is the GAL1 inducible promoter for use in Saccharomyces cerevisiae. It is repressed in the presence of glucose and induced in the presence of galactose. Raffinose is a neutral sugar source the neither induces nor represses the promoter. pGAL1 normally has an "all-or-nothing" response to galactose, but Christina Smolke has shown that knockout of the Gal2 gene gives pGAL1 a tunable, linear response to galactose.
mutable notes: none
mutable provenance: From the Saccharomyces cerevisiae genome, via Caroline Ajo-Franklin.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K165001_sequence/1

BBa_K165041
type: ComponentDefinition
persistent identity: BBa K165041
display id: BBa_K165041
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Zif268-HIV binding sites + TEF constitutive yeast promoter
creator: John Szymanski
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:10:56Z
discontinued: false
dominant: false
experience: experience/None
group u list: _267_
m user id: 0
owner id: 2512
owning group id: 58
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: -
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
component: BBa_K165037
component: BBa_K165011
sequence annotation: BBa_K165037
sequence annotation: BBa_K165011
sequence: https://synbiohub.org/public/igem/BBa_K165041_sequence/1

BBa_K165037
type: Component
persistent identity: component1996558
display id: component1996558
version: 1
top level: BBa_K165041
access: public
definition: pTEF

BBa_K165011
type: Component
persistent identity: component1996557
display id: component1996557
version: 1
top level: BBa_K165041
access: public
definition: https://synbiohub.org/public/igem/BBa_K165011/1

BBa_K165037
type: SequenceAnnotation
persistent identity: annotation1996558
display id: annotation1996558
version: 1
top level: BBa_K165041
location: https://synbiohub.org/public/igem/BBa_K165041/annotation1996558/range1996558/1
component: BBa_K165037

https://synbiohub.org/public/igem/BBa_K165041/annotation1996558/range1996558/1
type: Range
persistent identity: range1996558
display id: range1996558
version: 1
direction: direction/reverse
top level: BBa_K165041
start: 55
end: 457
orientation: inline

BBa_K165011
type: SequenceAnnotation
persistent identity: annotation1996557
display id: annotation1996557
version: 1
top level: BBa_K165041
location: https://synbiohub.org/public/igem/BBa_K165041/annotation1996557/range1996557/1
component: BBa_K165011

https://synbiohub.org/public/igem/BBa_K165041/annotation1996557/range1996557/1
type: Range
persistent identity: range1996557
display id: range1996557
version: 1
direction: direction/reverse
top level: BBa_K165041
start: 1
end: 46
orientation: inline

BBa_K165032
type: ComponentDefinition
persistent identity: BBa K165032
display id: BBa_K165032
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: mCYC promoter plus Gli1 binding sites
creator: Aaron Glieberman
created: 2008-10-28T12:00:00Z
modified: 2015-05-08T01:10:56Z
discontinued: false
dominant: false
group u list: _267_
m user id: 0
owner id: 2510
owning group id: 58
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This is a composite part containing the minimal promoter mCYC preceded by multiple consecutive binding sites for the Gli1 binding domain. This part contains the sequences for BBa_K165012 and BBa_K165016 but also has some additional DNA.
mutable notes: It was necessary to design primers for use in PCR to remove this part from its host plasmid and transfer it to a standard Biobrick vector.
mutable provenance: Composite part that was taken via PCR from a synthetic plasmid containing the sequence. The plasmid came from Lawrence Berkeley National Laboratory.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K165032_sequence/1

BBa_K105029
type: ComponentDefinition
persistent identity: BBa K105029
display id: BBa_K105029
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: cyc43 minimal promoter
creator: Michael Wild
created: 2008-10-25T11:00:00Z
modified: 2015-05-08T01:08:52Z
discontinued: false
dominant: false
experience: experience/None
group u list: _253_
m user id: 0
owner id: 3313
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This is a derivate of BBa_K105027. Due to a point mutation in the TATA box region the efficiency of this minimal promoter is only 43 % of the natural yeast CYC1 promoter.
mutable notes: This BioBrick contains the TATA-box with one point mutation and most of the 5'UTR (-139 to -36, relative to the translational start) of the natural CYC1 promoter. A Kozak sequence is not included and has thus to be added! Even if there isn't any need for this BioBrick to be fusible to other protein domains, we stayed with our design.
mutable provenance: Amplification and Mutation from the plasmid pAH3
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: TATA-box
sequence annotation: A -> G
sequence: https://synbiohub.org/public/igem/BBa_K105029_sequence/1

TATA-box
type: SequenceAnnotation
persistent identity: annotation1989837
display id: annotation1989837
version: 1
top level: BBa_K105029
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K105029/annotation1989837/range1989837/1

https://synbiohub.org/public/igem/BBa_K105029/annotation1989837/range1989837/1
type: Range
persistent identity: range1989837
display id: range1989837
version: 1
direction: direction/forward
top level: BBa_K105029
start: 17
end: 23
orientation: inline

A -> G
type: SequenceAnnotation
persistent identity: annotation1989838
display id: annotation1989838
version: 1
top level: BBa_K105029
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_K105029/annotation1989838/range1989838/1

https://synbiohub.org/public/igem/BBa_K105029/annotation1989838/range1989838/1
type: Range
persistent identity: range1989838
display id: range1989838
version: 1
direction: direction/forward
top level: BBa_K105029
start: 23
end: 23
orientation: inline

BBa_K124002
type: ComponentDefinition
persistent identity: BBa K124002
display id: BBa_K124002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Yeast GPD (TDH3) Promoter
creator: John Szymanski
created: 2008-06-30T11:00:00Z
modified: 2015-05-08T01:09:44Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _221_
m user id: 0
owner id: 2512
owning group id: 58
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Regulatory region spanning 680 bp upstream of the start codon of the GPD1 gene in yeast. Very strong constitutive promoter.
mutable notes: none
mutable provenance: Mumberg, Muller, and Funk. p413 pGPD http://dx.doi.org/10.1016/0378-1119(95)00037-7
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K124002_sequence/1

BBa_K106699
type: ComponentDefinition
persistent identity: BBa K106699
display id: BBa_K106699
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Gal1 Promoter
creator: Andrew Horwitz
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:09:00Z
discontinued: false
dominant: false
group u list: _226_
m user id: 0
owner id: 2659
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Galactose-inducible promoter (strong)!
mutable notes: None.
mutable provenance: Yeast genomic
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K106699_sequence/1

BBa_K110006
type: ComponentDefinition
persistent identity: BBa K110006
display id: BBa_K110006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Alpha-Cell Promoter MF(ALPHA)1
creator: James DiCarlo
created: 2008-05-29T11:00:00Z
modified: 2015-05-08T01:09:08Z
discontinued: false
dominant: false
experience: experience/None
group u list: _201_
m user id: 0
owner id: 2669
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This promoter was isolated by taking 500bp upstream of the MF(ALPHA)1 gene of S. cerevisiae. This promoter will be activated when alpha factor is expressed in an alpha haploid yeast cell.
mutable notes: This gene is on the Watson strand of the entered sequence.
mutable provenance: SGD
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: MCM1
sequence annotation: MF(alpha)1
sequence: https://synbiohub.org/public/igem/BBa_K110006_sequence/1

MCM1
type: SequenceAnnotation
persistent identity: annotation1997690
display id: annotation1997690
version: 1
top level: BBa_K110006
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K110006/annotation1997690/range1997690/1

https://synbiohub.org/public/igem/BBa_K110006/annotation1997690/range1997690/1
type: Range
persistent identity: range1997690
display id: range1997690
version: 1
direction: direction/forward
top level: BBa_K110006
start: 92
end: 102
orientation: inline

MF(alpha)1
type: SequenceAnnotation
persistent identity: annotation1997687
display id: annotation1997687
version: 1
top level: BBa_K110006
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K110006/annotation1997687/range1997687/1

https://synbiohub.org/public/igem/BBa_K110006/annotation1997687/range1997687/1
type: Range
persistent identity: range1997687
display id: range1997687
version: 1
direction: direction/forward
top level: BBa_K110006
start: 1
end: 501
orientation: inline

MCM1
type: SequenceAnnotation
persistent identity: annotation1997689
display id: annotation1997689
version: 1
top level: BBa_K110006
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K110006/annotation1997689/range1997689/1

https://synbiohub.org/public/igem/BBa_K110006/annotation1997689/range1997689/1
type: Range
persistent identity: range1997689
display id: range1997689
version: 1
direction: direction/forward
top level: BBa_K110006
start: 136
end: 161
orientation: inline

MCM1
type: SequenceAnnotation
persistent identity: annotation1997688
display id: annotation1997688
version: 1
top level: BBa_K110006
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K110006/annotation1997688/range1997688/1

https://synbiohub.org/public/igem/BBa_K110006/annotation1997688/range1997688/1
type: Range
persistent identity: range1997688
display id: range1997688
version: 1
direction: direction/forward
top level: BBa_K110006
start: 19
end: 34
orientation: inline

BBa_K165036
type: ComponentDefinition
persistent identity: BBa K165036
display id: BBa_K165036
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Gli1 bs + LexA bs + mCYC promoter
creator: John Szymanski
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:10:56Z
discontinued: false
dominant: false
group u list: _267_
m user id: 0
owner id: 2512
owning group id: 58
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: The Gli1 and LexA transcription factor binding sites on the mCYC minimal promoter (in that order.) Transcription regulation comes from Gli1 and LexA-binding transcription factors.
mutable notes: -
mutable provenance: Constructed in our lab with parts from Caroline Ajo-Franklin and David Drubin.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
component: BBa_K165012
component: BBa_K165031
sequence annotation: BBa_K165012
sequence annotation: BBa_K165031
sequence: https://synbiohub.org/public/igem/BBa_K165036_sequence/1

BBa_K165012
type: Component
persistent identity: component1995510
display id: component1995510
version: 1
top level: BBa_K165036
access: public
definition: https://synbiohub.org/public/igem/BBa_K165012/1

BBa_K165031
type: Component
persistent identity: component1995511
display id: component1995511
version: 1
top level: BBa_K165036
access: public
definition: BBa_K165031

BBa_K165012
type: SequenceAnnotation
persistent identity: annotation1995510
display id: annotation1995510
version: 1
top level: BBa_K165036
location: https://synbiohub.org/public/igem/BBa_K165036/annotation1995510/range1995510/1
component: BBa_K165012

https://synbiohub.org/public/igem/BBa_K165036/annotation1995510/range1995510/1
type: Range
persistent identity: range1995510
display id: range1995510
version: 1
direction: direction/reverse
top level: BBa_K165036
start: 1
end: 127
orientation: inline

BBa_K165031
type: SequenceAnnotation
persistent identity: annotation1995511
display id: annotation1995511
version: 1
top level: BBa_K165036
location: https://synbiohub.org/public/igem/BBa_K165036/annotation1995511/range1995511/1
component: BBa_K165031

https://synbiohub.org/public/igem/BBa_K165036/annotation1995511/range1995511/1
type: Range
persistent identity: range1995511
display id: range1995511
version: 1
direction: direction/reverse
top level: BBa_K165036
start: 136
end: 538
orientation: inline

BBa_K165043
type: ComponentDefinition
persistent identity: BBa K165043
display id: BBa_K165043
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Zif268-HIV binding sites + MET25 constitutive yeast promoter
creator: John Szymanski
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:10:56Z
discontinued: false
dominant: false
group u list: _267_
m user id: 0
owner id: 2512
owning group id: 58
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: -
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
component: BBa_K165011
component: BBa_K165000
sequence annotation: BBa_K165011
sequence annotation: BBa_K165000
sequence: https://synbiohub.org/public/igem/BBa_K165043_sequence/1

BBa_K165011
type: Component
persistent identity: component1996569
display id: component1996569
version: 1
top level: BBa_K165043
access: public
definition: https://synbiohub.org/public/igem/BBa_K165011/1

BBa_K165000
type: Component
persistent identity: component1996570
display id: component1996570
version: 1
top level: BBa_K165043
access: public
definition: BBa_K165000

BBa_K165011
type: SequenceAnnotation
persistent identity: annotation1996569
display id: annotation1996569
version: 1
top level: BBa_K165043
location: https://synbiohub.org/public/igem/BBa_K165043/annotation1996569/range1996569/1
component: BBa_K165011

https://synbiohub.org/public/igem/BBa_K165043/annotation1996569/range1996569/1
type: Range
persistent identity: range1996569
display id: range1996569
version: 1
direction: direction/reverse
top level: BBa_K165043
start: 1
end: 46
orientation: inline

BBa_K165000
type: SequenceAnnotation
persistent identity: annotation1996570
display id: annotation1996570
version: 1
top level: BBa_K165043
location: https://synbiohub.org/public/igem/BBa_K165043/annotation1996570/range1996570/1
component: BBa_K165000

https://synbiohub.org/public/igem/BBa_K165043/annotation1996570/range1996570/1
type: Range
persistent identity: range1996570
display id: range1996570
version: 1
direction: direction/reverse
top level: BBa_K165043
start: 55
end: 441
orientation: inline

BBa_K110015
type: ComponentDefinition
persistent identity: BBa K110015
display id: BBa_K110015
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: A-Cell Promoter MFA1 (RtL)
creator: Rick Carrick, Ambhi Ganesan, Alex McMillan
created: 2008-08-26T11:00:00Z
modified: 2015-05-08T01:09:09Z
discontinued: false
dominant: false
experience: experience/None
group u list: _201_
m user id: 0
owner id: 2669
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is the same sequence as MFA1 promotor(BBa_K110008), but with suffix and prefix switched
mutable notes: This is directlyh from the DNA sequence
mutable provenance: sgd
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: MCM1
sequence annotation: STE12
sequence annotation: MATalpha2
sequence annotation: MFA1 promoter (RtL)
sequence: https://synbiohub.org/public/igem/BBa_K110015_sequence/1

MCM1
type: SequenceAnnotation
persistent identity: annotation1981505
display id: annotation1981505
version: 1
top level: BBa_K110015
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K110015/annotation1981505/range1981505/1

https://synbiohub.org/public/igem/BBa_K110015/annotation1981505/range1981505/1
type: Range
persistent identity: range1981505
display id: range1981505
version: 1
direction: direction/forward
top level: BBa_K110015
start: 277
end: 287
orientation: inline

STE12
type: SequenceAnnotation
persistent identity: annotation1981506
display id: annotation1981506
version: 1
top level: BBa_K110015
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K110015/annotation1981506/range1981506/1

https://synbiohub.org/public/igem/BBa_K110015/annotation1981506/range1981506/1
type: Range
persistent identity: range1981506
display id: range1981506
version: 1
direction: direction/forward
top level: BBa_K110015
start: 302
end: 309
orientation: inline

MATalpha2
type: SequenceAnnotation
persistent identity: annotation1981504
display id: annotation1981504
version: 1
top level: BBa_K110015
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K110015/annotation1981504/range1981504/1

https://synbiohub.org/public/igem/BBa_K110015/annotation1981504/range1981504/1
type: Range
persistent identity: range1981504
display id: range1981504
version: 1
direction: direction/forward
top level: BBa_K110015
start: 267
end: 276
orientation: inline

MFA1 promoter (RtL)
type: SequenceAnnotation
persistent identity: annotation1981503
display id: annotation1981503
version: 1
top level: BBa_K110015
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K110015/annotation1981503/range1981503/1

https://synbiohub.org/public/igem/BBa_K110015/annotation1981503/range1981503/1
type: Range
persistent identity: range1981503
display id: range1981503
version: 1
direction: direction/forward
top level: BBa_K110015
start: 1
end: 401
orientation: inline

MATalpha2
type: SequenceAnnotation
persistent identity: annotation1981507
display id: annotation1981507
version: 1
top level: BBa_K110015
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K110015/annotation1981507/range1981507/1

https://synbiohub.org/public/igem/BBa_K110015/annotation1981507/range1981507/1
type: Range
persistent identity: range1981507
display id: range1981507
version: 1
direction: direction/forward
top level: BBa_K110015
start: 289
end: 297
orientation: inline

BBa_I766558
type: ComponentDefinition
persistent identity: BBa I766558
display id: BBa_I766558
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pFig1 (Inducible) Promoter
creator: Nili Sommovilla
created: 2007-08-15T11:00:00Z
modified: 2015-08-31T04:08:10Z
discontinued: false
dominant: false
experience: experience/None
group u list: _155_
m user id: 0
owner id: 1931
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Inducible promoter in yeast
mutable notes: none
mutable provenance: endogenous promoter for Fig1 (Factor Induced Gene 1)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I766558_sequence/1

BBa_K165030
type: ComponentDefinition
persistent identity: BBa K165030
display id: BBa_K165030
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: mCYC promoter plus Zif268-HIV binding sites
creator: Aaron Glieberman
created: 2008-10-28T12:00:00Z
modified: 2015-05-08T01:10:56Z
discontinued: false
dominant: false
group u list: _267_
m user id: 0
owner id: 2510
owning group id: 58
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This is a composite part containing the minimal promoter mCYC preceded by multiple consecutive binding sites for the Zif268-HIV binding domain. It contains the sequences of parts BBa_K165011 and BBa_K165016 but also contains additional DNA.
mutable notes: It was necessary to design primers for use in PCR to remove this part from its host plasmid and transfer it to a standard Biobrick vector.
mutable provenance: Composite part that was taken via PCR from a synthetic plasmid containing the sequence. The plasmid came from Lawrence Berkeley National Laboratory.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: Zif268-HIV binding sites (3)
sequence: https://synbiohub.org/public/igem/BBa_K165030_sequence/1

Zif268-HIV binding sites (3)
type: SequenceAnnotation
persistent identity: annotation1994037
display id: annotation1994037
version: 1
top level: BBa_K165030
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K165030/annotation1994037/range1994037/1

https://synbiohub.org/public/igem/BBa_K165030/annotation1994037/range1994037/1
type: Range
persistent identity: range1994037
display id: range1994037
version: 1
direction: direction/forward
top level: BBa_K165030
start: 1
end: 46
orientation: inline

BBa_M31201
type: ComponentDefinition
persistent identity: BBa M31201
display id: BBa_M31201
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Yeast CLB1 promoter region, G2/M cell cycle specific
creator: Katherine Aull
created: 2008-05-13T11:00:00Z
modified: 2015-05-08T01:13:59Z
discontinued: false
dominant: false
group u list: _89_
m user id: 0
owner id: 624
owning group id: 89
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Description of CLB1 (from SGD): B-type cyclin involved in cell cycle progression; activates Cdc28p to promote the transition from G2 to M phase; accumulates during G2 and M, then targeted via a destruction box motif for ubiquitin-mediated degradation by the proteasome. More information found here: http://db.yeastgenome.org/cgi-bin/locus.pl?locus=CLB1
mutable notes: Part contains 500 bases upstream from the CLB1 ORF. Is NOT a minimal promoter, and has not been tested for composability.
mutable provenance: Yeast consensus sequence from SGD chrVII:703140..703639
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_M31201_sequence/1

BBa_K165034
type: ComponentDefinition
persistent identity: BBa K165034
display id: BBa_K165034
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Zif268-HIV bs + LexA bs + mCYC promoter
creator: John Szymanski
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:10:56Z
discontinued: false
dominant: false
group u list: _267_
m user id: 0
owner id: 2512
owning group id: 58
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: The Zif268-HIV and LexA transcription factor binding sites on the mCYC minimal promoter (in that order.) Transcription regulation comes from Zif268-HIV and LexA-binding transcription factors.
mutable notes: -
mutable provenance: Constructed in our lab with parts from Caroline Ajo-Franklin and David Drubin.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
component: BBa_K165011
component: BBa_K165031
sequence annotation: BBa_K165031
sequence annotation: BBa_K165011
sequence: https://synbiohub.org/public/igem/BBa_K165034_sequence/1

BBa_K165011
type: Component
persistent identity: component1995464
display id: component1995464
version: 1
top level: BBa_K165034
access: public
definition: https://synbiohub.org/public/igem/BBa_K165011/1

BBa_K165031
type: Component
persistent identity: component1995465
display id: component1995465
version: 1
top level: BBa_K165034
access: public
definition: BBa_K165031

BBa_K165031
type: SequenceAnnotation
persistent identity: annotation1995465
display id: annotation1995465
version: 1
top level: BBa_K165034
location: https://synbiohub.org/public/igem/BBa_K165034/annotation1995465/range1995465/1
component: BBa_K165031

https://synbiohub.org/public/igem/BBa_K165034/annotation1995465/range1995465/1
type: Range
persistent identity: range1995465
display id: range1995465
version: 1
direction: direction/reverse
top level: BBa_K165034
start: 55
end: 457
orientation: inline

BBa_K165011
type: SequenceAnnotation
persistent identity: annotation1995464
display id: annotation1995464
version: 1
top level: BBa_K165034
location: https://synbiohub.org/public/igem/BBa_K165034/annotation1995464/range1995464/1
component: BBa_K165011

https://synbiohub.org/public/igem/BBa_K165034/annotation1995464/range1995464/1
type: Range
persistent identity: range1995464
display id: range1995464
version: 1
direction: direction/reverse
top level: BBa_K165034
start: 1
end: 46
orientation: inline

BBa_K122000
type: ComponentDefinition
persistent identity: BBa K122000
display id: BBa_K122000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pPGK1
creator: David Ouyang
created: 2008-10-20T11:00:00Z
modified: 2015-05-08T01:09:42Z
discontinued: false
dominant: false
experience: experience/None
group u list: _218_
m user id: 0
owner id: 3326
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This is the 1500 bp upstream of the yeast PGK1 coding region. This is a putative constitutive promoter.
mutable notes: Source: Saccharomyces cerevisiae genomic DNA.
mutable provenance: This part was obtained from yeast genomic DNA.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K122000_sequence/1

BBa_K165040
type: ComponentDefinition
persistent identity: BBa K165040
display id: BBa_K165040
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Gli1 binding sites + TEF constitutive yeast promoter
creator: John Szymanski
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:10:56Z
discontinued: false
dominant: false
group u list: _267_
m user id: 0
owner id: 2512
owning group id: 58
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: The Gli1 transcription factor binding sites on the TEF2 constitutive promoter. Transcription regulation should be constitutive plus regulated by Gli1-binding transcription factors.
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
component: BBa_K165012
component: BBa_K165037
sequence annotation: BBa_K165037
sequence annotation: BBa_K165012
sequence: https://synbiohub.org/public/igem/BBa_K165040_sequence/1

BBa_K165012
type: Component
persistent identity: component1996555
display id: component1996555
version: 1
top level: BBa_K165040
access: public
definition: https://synbiohub.org/public/igem/BBa_K165012/1

BBa_K165037
type: Component
persistent identity: component1996556
display id: component1996556
version: 1
top level: BBa_K165040
access: public
definition: pTEF

BBa_K165037
type: SequenceAnnotation
persistent identity: annotation1996556
display id: annotation1996556
version: 1
top level: BBa_K165040
location: https://synbiohub.org/public/igem/BBa_K165040/annotation1996556/range1996556/1
component: BBa_K165037

https://synbiohub.org/public/igem/BBa_K165040/annotation1996556/range1996556/1
type: Range
persistent identity: range1996556
display id: range1996556
version: 1
direction: direction/reverse
top level: BBa_K165040
start: 136
end: 538
orientation: inline

BBa_K165012
type: SequenceAnnotation
persistent identity: annotation1996555
display id: annotation1996555
version: 1
top level: BBa_K165040
location: https://synbiohub.org/public/igem/BBa_K165040/annotation1996555/range1996555/1
component: BBa_K165012

https://synbiohub.org/public/igem/BBa_K165040/annotation1996555/range1996555/1
type: Range
persistent identity: range1996555
display id: range1996555
version: 1
direction: direction/reverse
top level: BBa_K165040
start: 1
end: 127
orientation: inline

P0310
type: ComponentDefinition
persistent identity: BBa J63005
display id: BBa_J63005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: yeast ADH1 promoter
creator: Caroline Ajo-Franklin
created: 2006-10-10T11:00:00Z
modified: 2015-08-31T01:56:26Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _97_
m user id: 0
owner id: 545
owning group id: 97
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: ADH1 promoter from S. cerevisiae
mutable notes: no changes to genomic sequence
mutable provenance: genomic DNA of ADH1 from S. cerevisiae
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: Constitutive promoter
sequence annotation: TATA Box
sequence: https://synbiohub.org/public/igem/BBa_J63005_sequence/1

Constitutive promoter
type: SequenceAnnotation
persistent identity: annotation1902744
display id: annotation1902744
version: 1
top level: P0310
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J63005/annotation1902744/range1902744/1

https://synbiohub.org/public/igem/BBa_J63005/annotation1902744/range1902744/1
type: Range
persistent identity: range1902744
display id: range1902744
version: 1
direction: direction/forward
top level: P0310
start: 1
end: 1445
orientation: inline

TATA Box
type: SequenceAnnotation
persistent identity: annotation1902745
display id: annotation1902745
version: 1
top level: P0310
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_J63005/annotation1902745/range1902745/1

https://synbiohub.org/public/igem/BBa_J63005/annotation1902745/range1902745/1
type: Range
persistent identity: range1902745
display id: range1902745
version: 1
direction: direction/forward
top level: P0310
start: 1348
end: 1354
orientation: inline

BBa_K105028
type: ComponentDefinition
persistent identity: BBa K105028
display id: BBa_K105028
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: cyc70 minimal promoter
creator: Michael Wild
created: 2008-10-25T11:00:00Z
modified: 2015-05-08T01:08:52Z
discontinued: false
dominant: false
experience: experience/None
group u list: _253_
m user id: 0
owner id: 3313
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This is a derivate of BBa_K105027. Due to a point mutation in the TATA box region the efficiency of this minimal promoter is only 70 % of the natural yeast CYC1 promoter.
mutable notes: This BioBrick contains the TATA-box with one point mutation and most of the 5'UTR (-139 to -36, relative to the translational start) of the natural CYC1 promoter. A Kozak sequence is not included and has thus to be added! Even if there isn't any need for this BioBrick to be fusible to other protein domains, we stayed with our design.
mutable provenance: Amplification and Mutation from the plasmid pAH3
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: A->C
sequence annotation: TATA-box
sequence: https://synbiohub.org/public/igem/BBa_K105028_sequence/1

A->C
type: SequenceAnnotation
persistent identity: annotation1989836
display id: annotation1989836
version: 1
top level: BBa_K105028
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_K105028/annotation1989836/range1989836/1

https://synbiohub.org/public/igem/BBa_K105028/annotation1989836/range1989836/1
type: Range
persistent identity: range1989836
display id: range1989836
version: 1
direction: direction/forward
top level: BBa_K105028
start: 24
end: 24
orientation: inline

TATA-box
type: SequenceAnnotation
persistent identity: annotation1989835
display id: annotation1989835
version: 1
top level: BBa_K105028
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K105028/annotation1989835/range1989835/1

https://synbiohub.org/public/igem/BBa_K105028/annotation1989835/range1989835/1
type: Range
persistent identity: range1989835
display id: range1989835
version: 1
direction: direction/forward
top level: BBa_K105028
start: 17
end: 23
orientation: inline

BBa_K165035
type: ComponentDefinition
persistent identity: BBa K165035
display id: BBa_K165035
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Gli1 bs + Zif268-HIV bs + mCYC promoter
creator: John Szymanski
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:10:56Z
discontinued: false
dominant: false
group u list: _267_
m user id: 0
owner id: 2512
owning group id: 58
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: The Gli1 and Zif268-HIV transcription factor binding sites on the mCYC minimal promoter (in that order.) Transcription regulation comes from Gli1 and Zif268-HIV binding transcription factors.
mutable notes: -
mutable provenance: Constructed in our lab with parts from Caroline Ajo-Franklin and David Drubin.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
component: BBa_K165030
component: BBa_K165012
sequence annotation: BBa_K165012
sequence annotation: BBa_K165030
sequence: https://synbiohub.org/public/igem/BBa_K165035_sequence/1

BBa_K165030
type: Component
persistent identity: component1995497
display id: component1995497
version: 1
top level: BBa_K165035
access: public
definition: BBa_K165030

BBa_K165012
type: Component
persistent identity: component1995495
display id: component1995495
version: 1
top level: BBa_K165035
access: public
definition: https://synbiohub.org/public/igem/BBa_K165012/1

BBa_K165012
type: SequenceAnnotation
persistent identity: annotation1995495
display id: annotation1995495
version: 1
top level: BBa_K165035
location: https://synbiohub.org/public/igem/BBa_K165035/annotation1995495/range1995495/1
component: BBa_K165012

https://synbiohub.org/public/igem/BBa_K165035/annotation1995495/range1995495/1
type: Range
persistent identity: range1995495
display id: range1995495
version: 1
direction: direction/reverse
top level: BBa_K165035
start: 1
end: 127
orientation: inline

BBa_K165030
type: SequenceAnnotation
persistent identity: annotation1995497
display id: annotation1995497
version: 1
top level: BBa_K165035
location: https://synbiohub.org/public/igem/BBa_K165035/annotation1995497/range1995497/1
component: BBa_K165030

https://synbiohub.org/public/igem/BBa_K165035/annotation1995497/range1995497/1
type: Range
persistent identity: range1995497
display id: range1995497
version: 1
direction: direction/reverse
top level: BBa_K165035
start: 136
end: 442
orientation: inline

BBa_K110005
type: ComponentDefinition
persistent identity: BBa K110005
display id: BBa_K110005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Alpha-Cell Promoter MF(ALPHA)2
creator: James DiCarlo
created: 2008-05-29T11:00:00Z
modified: 2015-05-08T01:09:08Z
discontinued: false
dominant: false
experience: experience/None
group u list: _201_
m user id: 0
owner id: 2669
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This promoter was isolated by taking 500bp upstream of the MF(ALPHA)2 gene of S. cerevisiae. This promoter will be activated when alpha factor is expressed in an alpha haploid yeast cell.
mutable notes: This gene is on the crick strand of the entered sequence.
mutable provenance: SGD
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: MCM1
sequence annotation: MF(alpha)2
sequence: https://synbiohub.org/public/igem/BBa_K110005_sequence/1

MCM1
type: SequenceAnnotation
persistent identity: annotation1997684
display id: annotation1997684
version: 1
top level: BBa_K110005
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K110005/annotation1997684/range1997684/1

https://synbiohub.org/public/igem/BBa_K110005/annotation1997684/range1997684/1
type: Range
persistent identity: range1997684
display id: range1997684
version: 1
direction: direction/forward
top level: BBa_K110005
start: 184
end: 209
orientation: inline

MF(alpha)2
type: SequenceAnnotation
persistent identity: annotation1997686
display id: annotation1997686
version: 1
top level: BBa_K110005
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K110005/annotation1997686/range1997686/1

https://synbiohub.org/public/igem/BBa_K110005/annotation1997686/range1997686/1
type: Range
persistent identity: range1997686
display id: range1997686
version: 1
direction: direction/forward
top level: BBa_K110005
start: 1
end: 500
orientation: inline

pTEF
type: ComponentDefinition
persistent identity: BBa K165037
display id: BBa_K165037
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TEF2 yeast constitutive promoter
creator: John Szymanski
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:10:56Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _267_
m user id: 0
owner id: 2512
owning group id: 58
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: This is a high level constitutive promoter from the yeast gene TEF2.
mutable notes: -
mutable provenance: From the yeast genome using promoter definition from: Mumberg, Muller, and Funk: http://dx.doi.org/10.1016/0378-1119(95)00037-7
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K165037_sequence/1

BBa_K165033
type: ComponentDefinition
persistent identity: BBa K165033
display id: BBa_K165033
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: YY1 binding sites + mCYC promoter
creator: John Szymanski
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:10:56Z
discontinued: false
dominant: false
group u list: _267_
m user id: 0
owner id: 2512
owning group id: 58
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: The YY1 transcription factor binding sites on the mCYC minimal promoter. All transcription regulation comes from YY1-binding transcription factors.
mutable notes: -
mutable provenance: Made in our lab with parts from Caroline Ajo-Franklin and David Drubin.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
component: BBa_K165016
component: BBa_K165013
sequence annotation: BBa_K165013
sequence annotation: BBa_K165016
sequence: https://synbiohub.org/public/igem/BBa_K165033_sequence/1

BBa_K165016
type: Component
persistent identity: component1995403
display id: component1995403
version: 1
top level: BBa_K165033
access: public
definition: BBa_K165016

BBa_K165013
type: Component
persistent identity: component1995402
display id: component1995402
version: 1
top level: BBa_K165033
access: public
definition: https://synbiohub.org/public/igem/BBa_K165013/1

BBa_K165013
type: SequenceAnnotation
persistent identity: annotation1995402
display id: annotation1995402
version: 1
top level: BBa_K165033
location: https://synbiohub.org/public/igem/BBa_K165033/annotation1995402/range1995402/1
component: BBa_K165013

https://synbiohub.org/public/igem/BBa_K165033/annotation1995402/range1995402/1
type: Range
persistent identity: range1995402
display id: range1995402
version: 1
direction: direction/reverse
top level: BBa_K165033
start: 1
end: 51
orientation: inline

BBa_K165016
type: SequenceAnnotation
persistent identity: annotation1995403
display id: annotation1995403
version: 1
top level: BBa_K165033
location: https://synbiohub.org/public/igem/BBa_K165033/annotation1995403/range1995403/1
component: BBa_K165016

https://synbiohub.org/public/igem/BBa_K165033/annotation1995403/range1995403/1
type: Range
persistent identity: range1995403
display id: range1995403
version: 1
direction: direction/reverse
top level: BBa_K165033
start: 60
end: 304
orientation: inline

BBa_I766214
type: ComponentDefinition
persistent identity: BBa I766214
display id: BBa_I766214
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pGal1
creator: Nili Sommovilla
created: 2007-10-21T11:00:00Z
modified: 2015-08-31T04:08:09Z
discontinued: false
dominant: false
group u list: _155_
m user id: 0
owner id: 1931
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: pGAl1 promoter region
mutable notes: none
mutable provenance: yeast
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I766214_sequence/1

BBa_K110016
type: ComponentDefinition
persistent identity: BBa K110016
display id: BBa_K110016
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: A-Cell Promoter STE2 (backwards)
creator: James DiCarlo
created: 2008-08-26T11:00:00Z
modified: 2015-05-08T01:09:09Z
discontinued: false
dominant: false
experience: experience/None
group u list: _201_
m user id: 0
owner id: 2669
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This is the same sequence as STE2 promotor(BBa_K110008), but with suffix and prefix switched
mutable notes: This is directly from the DNA sequence
mutable provenance: sgd
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: MATalpha2
sequence annotation: MCM1
sequence annotation: STE2 Promoter
sequence annotation: STE12
sequence: https://synbiohub.org/public/igem/BBa_K110016_sequence/1

MATalpha2
type: SequenceAnnotation
persistent identity: annotation1981091
display id: annotation1981091
version: 1
top level: BBa_K110016
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K110016/annotation1981091/range1981091/1

https://synbiohub.org/public/igem/BBa_K110016/annotation1981091/range1981091/1
type: Range
persistent identity: range1981091
display id: range1981091
version: 1
direction: direction/forward
top level: BBa_K110016
start: 273
end: 282
orientation: inline

MATalpha2
type: SequenceAnnotation
persistent identity: annotation1981093
display id: annotation1981093
version: 1
top level: BBa_K110016
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K110016/annotation1981093/range1981093/1

https://synbiohub.org/public/igem/BBa_K110016/annotation1981093/range1981093/1
type: Range
persistent identity: range1981093
display id: range1981093
version: 1
direction: direction/forward
top level: BBa_K110016
start: 295
end: 302
orientation: inline

MCM1
type: SequenceAnnotation
persistent identity: annotation1981092
display id: annotation1981092
version: 1
top level: BBa_K110016
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K110016/annotation1981092/range1981092/1

https://synbiohub.org/public/igem/BBa_K110016/annotation1981092/range1981092/1
type: Range
persistent identity: range1981092
display id: range1981092
version: 1
direction: direction/forward
top level: BBa_K110016
start: 284
end: 293
orientation: inline

STE2 Promoter
type: SequenceAnnotation
persistent identity: annotation1981094
display id: annotation1981094
version: 1
top level: BBa_K110016
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K110016/annotation1981094/range1981094/1

https://synbiohub.org/public/igem/BBa_K110016/annotation1981094/range1981094/1
type: Range
persistent identity: range1981094
display id: range1981094
version: 1
direction: direction/forward
top level: BBa_K110016
start: 1
end: 500
orientation: inline

STE12
type: SequenceAnnotation
persistent identity: annotation1981095
display id: annotation1981095
version: 1
top level: BBa_K110016
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K110016/annotation1981095/range1981095/1

https://synbiohub.org/public/igem/BBa_K110016/annotation1981095/range1981095/1
type: Range
persistent identity: range1981095
display id: range1981095
version: 1
direction: direction/forward
top level: BBa_K110016
start: 304
end: 311
orientation: inline

BBa_I766556
type: ComponentDefinition
persistent identity: BBa I766556
display id: BBa_I766556
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pAdh (Strong) Promoter
creator: Nili Sommovilla
created: 2007-08-15T11:00:00Z
modified: 2015-08-31T04:08:10Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _155_
m user id: 0
owner id: 1931
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Strong Expression level Constitutive promoter in yeast
mutable notes: none
mutable provenance: Endogenous promoter for Alcohol Dehydrogenase
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I766556_sequence/1

BBa_K110008
type: ComponentDefinition
persistent identity: BBa K110008
display id: BBa_K110008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: A-Cell Promoter MFA1
creator: James DiCarlo
created: 2008-05-29T11:00:00Z
modified: 2015-05-08T01:09:09Z
discontinued: false
dominant: false
experience: experience/None
group u list: _201_
m user id: 0
owner id: 2669
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This promoter was isolated by taking 500bp upstream of the MFA1 gene of S. cerevisiae. This promoter will be activated when A factor is expressed in an A haploid yeast cell.
mutable notes: This gene is on the Watson strand of the entered sequence.
mutable provenance: SGD
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: MFA1 Promoter
sequence annotation: MATalpha2
sequence annotation: STE12
sequence annotation: MCM1
sequence: https://synbiohub.org/public/igem/BBa_K110008_sequence/1

MFA1 Promoter
type: SequenceAnnotation
persistent identity: annotation1981086
display id: annotation1981086
version: 1
top level: BBa_K110008
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K110008/annotation1981086/range1981086/1

https://synbiohub.org/public/igem/BBa_K110008/annotation1981086/range1981086/1
type: Range
persistent identity: range1981086
display id: range1981086
version: 1
direction: direction/forward
top level: BBa_K110008
start: 1
end: 501
orientation: inline

MATalpha2
type: SequenceAnnotation
persistent identity: annotation1981087
display id: annotation1981087
version: 1
top level: BBa_K110008
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K110008/annotation1981087/range1981087/1

https://synbiohub.org/public/igem/BBa_K110008/annotation1981087/range1981087/1
type: Range
persistent identity: range1981087
display id: range1981087
version: 1
direction: direction/forward
top level: BBa_K110008
start: 267
end: 276
orientation: inline

STE12
type: SequenceAnnotation
persistent identity: annotation1981090
display id: annotation1981090
version: 1
top level: BBa_K110008
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K110008/annotation1981090/range1981090/1

https://synbiohub.org/public/igem/BBa_K110008/annotation1981090/range1981090/1
type: Range
persistent identity: range1981090
display id: range1981090
version: 1
direction: direction/forward
top level: BBa_K110008
start: 302
end: 309
orientation: inline

MCM1
type: SequenceAnnotation
persistent identity: annotation1981088
display id: annotation1981088
version: 1
top level: BBa_K110008
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K110008/annotation1981088/range1981088/1

https://synbiohub.org/public/igem/BBa_K110008/annotation1981088/range1981088/1
type: Range
persistent identity: range1981088
display id: range1981088
version: 1
direction: direction/forward
top level: BBa_K110008
start: 277
end: 287
orientation: inline

MATalpha2
type: SequenceAnnotation
persistent identity: annotation1981089
display id: annotation1981089
version: 1
top level: BBa_K110008
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K110008/annotation1981089/range1981089/1

https://synbiohub.org/public/igem/BBa_K110008/annotation1981089/range1981089/1
type: Range
persistent identity: range1981089
display id: range1981089
version: 1
direction: direction/forward
top level: BBa_K110008
start: 289
end: 297
orientation: inline

BBa_K165042
type: ComponentDefinition
persistent identity: BBa K165042
display id: BBa_K165042
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Gli1 binding sites + MET25 inducible yeast promoter
creator: John Szymanski
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:10:56Z
discontinued: false
dominant: false
group u list: _267_
m user id: 0
owner id: 2512
owning group id: 58
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: -
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
component: BBa_K165012
component: BBa_K165000
sequence annotation: BBa_K165000
sequence annotation: BBa_K165012
sequence: https://synbiohub.org/public/igem/BBa_K165042_sequence/1

BBa_K165012
type: Component
persistent identity: component1996562
display id: component1996562
version: 1
top level: BBa_K165042
access: public
definition: https://synbiohub.org/public/igem/BBa_K165012/1

BBa_K165000
type: Component
persistent identity: component1996563
display id: component1996563
version: 1
top level: BBa_K165042
access: public
definition: BBa_K165000

BBa_K165000
type: SequenceAnnotation
persistent identity: annotation1996563
display id: annotation1996563
version: 1
top level: BBa_K165042
location: https://synbiohub.org/public/igem/BBa_K165042/annotation1996563/range1996563/1
component: BBa_K165000

https://synbiohub.org/public/igem/BBa_K165042/annotation1996563/range1996563/1
type: Range
persistent identity: range1996563
display id: range1996563
version: 1
direction: direction/reverse
top level: BBa_K165042
start: 136
end: 522
orientation: inline

BBa_K165012
type: SequenceAnnotation
persistent identity: annotation1996562
display id: annotation1996562
version: 1
top level: BBa_K165042
location: https://synbiohub.org/public/igem/BBa_K165042/annotation1996562/range1996562/1
component: BBa_K165012

https://synbiohub.org/public/igem/BBa_K165042/annotation1996562/range1996562/1
type: Range
persistent identity: range1996562
display id: range1996562
version: 1
direction: direction/reverse
top level: BBa_K165042
start: 1
end: 127
orientation: inline

BBa_K165039
type: ComponentDefinition
persistent identity: BBa K165039
display id: BBa_K165039
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Zif268-HIV binding sites + ADH1 yeast promoter
creator: John Szymanski
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:10:56Z
discontinued: false
dominant: false
group u list: _267_
m user id: 0
owner id: 2512
owning group id: 58
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: The Zif268-HIV transcription factor binding sites on the ADH1 constitutive promoter. Transcription regulation should be constitutive plus regulated by Zif268-HIV-binding transcription factors.
mutable notes: -
mutable provenance: Constructed in our lab with parts from Caroline Ajo-Franklin and David Drubin.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
component: BBa_K165015
component: BBa_K165011
sequence annotation: BBa_K165015
sequence annotation: BBa_K165011
sequence: https://synbiohub.org/public/igem/BBa_K165039_sequence/1

BBa_K165015
type: Component
persistent identity: component1995517
display id: component1995517
version: 1
top level: BBa_K165039
access: public
definition: BBa_K165015

BBa_K165011
type: Component
persistent identity: component1995516
display id: component1995516
version: 1
top level: BBa_K165039
access: public
definition: https://synbiohub.org/public/igem/BBa_K165011/1

BBa_K165015
type: SequenceAnnotation
persistent identity: annotation1995517
display id: annotation1995517
version: 1
top level: BBa_K165039
location: https://synbiohub.org/public/igem/BBa_K165039/annotation1995517/range1995517/1
component: BBa_K165015

https://synbiohub.org/public/igem/BBa_K165039/annotation1995517/range1995517/1
type: Range
persistent identity: range1995517
display id: range1995517
version: 1
direction: direction/reverse
top level: BBa_K165039
start: 55
end: 1499
orientation: inline

BBa_K165011
type: SequenceAnnotation
persistent identity: annotation1995516
display id: annotation1995516
version: 1
top level: BBa_K165039
location: https://synbiohub.org/public/igem/BBa_K165039/annotation1995516/range1995516/1
component: BBa_K165011

https://synbiohub.org/public/igem/BBa_K165039/annotation1995516/range1995516/1
type: Range
persistent identity: range1995516
display id: range1995516
version: 1
direction: direction/reverse
top level: BBa_K165039
start: 1
end: 46
orientation: inline

BBa_K124000
type: ComponentDefinition
persistent identity: BBa K124000
display id: BBa_K124000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pCYC Yeast Promoter
creator: John Szymanski
created: 2008-06-30T11:00:00Z
modified: 2015-05-08T01:09:44Z
discontinued: false
dominant: false
group u list: _221_
m user id: 0
owner id: 2512
owning group id: 58
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: The promoter from the yeast gene CYC1. Activity characterized here: http://dx.doi.org/10.1016/0378-1119(95)00037-7.
mutable notes: none
mutable provenance: From Mumberg, Muller, and Funk p413 pCYC. See ref. above
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K124000_sequence/1

BBa_K165038
type: ComponentDefinition
persistent identity: BBa K165038
display id: BBa_K165038
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Gli1 binding sites + ADH1 constitutive yeast promoter
creator: John Szymanski
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:10:56Z
discontinued: false
dominant: false
group u list: _267_
m user id: 0
owner id: 2512
owning group id: 58
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: The Gli1 transcription factor binding sites on the ADH1 constitutive promoter. Transcription regulation should be constitutive plus regulated by YY1-binding transcription factors.
mutable notes: -
mutable provenance: Constructed in our lab with parts from Caroline Ajo-Franklin and David Drubin.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
component: BBa_K165012
component: BBa_K165015
sequence annotation: BBa_K165015
sequence annotation: BBa_K165012
sequence: https://synbiohub.org/public/igem/BBa_K165038_sequence/1

BBa_K165012
type: Component
persistent identity: component1995512
display id: component1995512
version: 1
top level: BBa_K165038
access: public
definition: https://synbiohub.org/public/igem/BBa_K165012/1

BBa_K165015
type: Component
persistent identity: component1995513
display id: component1995513
version: 1
top level: BBa_K165038
access: public
definition: BBa_K165015

BBa_K165015
type: SequenceAnnotation
persistent identity: annotation1995513
display id: annotation1995513
version: 1
top level: BBa_K165038
location: https://synbiohub.org/public/igem/BBa_K165038/annotation1995513/range1995513/1
component: BBa_K165015

https://synbiohub.org/public/igem/BBa_K165038/annotation1995513/range1995513/1
type: Range
persistent identity: range1995513
display id: range1995513
version: 1
direction: direction/reverse
top level: BBa_K165038
start: 136
end: 1580
orientation: inline

BBa_K165012
type: SequenceAnnotation
persistent identity: annotation1995512
display id: annotation1995512
version: 1
top level: BBa_K165038
location: https://synbiohub.org/public/igem/BBa_K165038/annotation1995512/range1995512/1
component: BBa_K165012

https://synbiohub.org/public/igem/BBa_K165038/annotation1995512/range1995512/1
type: Range
persistent identity: range1995512
display id: range1995512
version: 1
direction: direction/reverse
top level: BBa_K165038
start: 1
end: 127
orientation: inline

BBa_K110009
type: ComponentDefinition
persistent identity: BBa K110009
display id: BBa_K110009
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: A-Cell Promoter STE2
creator: Rick Carrick, Ambhi Ganesan, Alex McMillan
created: 2008-05-29T11:00:00Z
modified: 2015-05-08T01:09:09Z
discontinued: false
dominant: false
experience: experience/None
group u list: _201_
m user id: 0
owner id: 2669
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This promoter was isolated by taking 500bp upstream of the MFA1 gene of S. cerevisiae. This promoter will be activated when A factor is expressed in an A haploid yeast cell.
mutable notes: This promotor is on the crick strand of the entered sequence.
mutable provenance: SGD
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: STE12
sequence annotation: Mat Alpha2
sequence annotation: Ste2 Promoter
sequence annotation: MCM1
sequence: https://synbiohub.org/public/igem/BBa_K110009_sequence/1

STE12
type: SequenceAnnotation
persistent identity: annotation1981502
display id: annotation1981502
version: 1
top level: BBa_K110009
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K110009/annotation1981502/range1981502/1

https://synbiohub.org/public/igem/BBa_K110009/annotation1981502/range1981502/1
type: Range
persistent identity: range1981502
display id: range1981502
version: 1
direction: direction/forward
top level: BBa_K110009
start: 304
end: 312
orientation: inline

Mat Alpha2
type: SequenceAnnotation
persistent identity: annotation1981499
display id: annotation1981499
version: 1
top level: BBa_K110009
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K110009/annotation1981499/range1981499/1

https://synbiohub.org/public/igem/BBa_K110009/annotation1981499/range1981499/1
type: Range
persistent identity: range1981499
display id: range1981499
version: 1
direction: direction/forward
top level: BBa_K110009
start: 273
end: 280
orientation: inline

Ste2 Promoter
type: SequenceAnnotation
persistent identity: annotation1981498
display id: annotation1981498
version: 1
top level: BBa_K110009
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K110009/annotation1981498/range1981498/1

https://synbiohub.org/public/igem/BBa_K110009/annotation1981498/range1981498/1
type: Range
persistent identity: range1981498
display id: range1981498
version: 1
direction: direction/forward
top level: BBa_K110009
start: 1
end: 501
orientation: inline

Mat Alpha2
type: SequenceAnnotation
persistent identity: annotation1981501
display id: annotation1981501
version: 1
top level: BBa_K110009
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K110009/annotation1981501/range1981501/1

https://synbiohub.org/public/igem/BBa_K110009/annotation1981501/range1981501/1
type: Range
persistent identity: range1981501
display id: range1981501
version: 1
direction: direction/forward
top level: BBa_K110009
start: 295
end: 301
orientation: inline

MCM1
type: SequenceAnnotation
persistent identity: annotation1981500
display id: annotation1981500
version: 1
top level: BBa_K110009
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K110009/annotation1981500/range1981500/1

https://synbiohub.org/public/igem/BBa_K110009/annotation1981500/range1981500/1
type: Range
persistent identity: range1981500
display id: range1981500
version: 1
direction: direction/forward
top level: BBa_K110009
start: 283
end: 292
orientation: inline

BBa_K105031
type: ComponentDefinition
persistent identity: BBa K105031
display id: BBa_K105031
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: cyc16 minimal promoter
creator: Michael Wild
created: 2008-10-25T11:00:00Z
modified: 2015-05-08T01:08:52Z
discontinued: false
dominant: false
experience: experience/None
group u list: _253_
m user id: 0
owner id: 3313
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This is a derivate of BBa_K105027. Due to a point mutation in the TATA box region the efficiency of this minimal promoter is only 16 % of the natural yeast CYC1 promoter.
mutable notes: This BioBrick contains the TATA-box with one point mutation and most of the 5'UTR (-139 to -36, relative to the translational start) of the natural CYC1 promoter. A Kozak sequence is not included and has thus to be added! Even if there isn't any need for this BioBrick to be fusible to other protein domains, we stayed with our design.
mutable provenance: Amplification and Mutation from the plasmid pAH3
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: A -> G
sequence annotation: TATA-box
sequence: https://synbiohub.org/public/igem/BBa_K105031_sequence/1

A -> G
type: SequenceAnnotation
persistent identity: annotation1989845
display id: annotation1989845
version: 1
top level: BBa_K105031
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_K105031/annotation1989845/range1989845/1

https://synbiohub.org/public/igem/BBa_K105031/annotation1989845/range1989845/1
type: Range
persistent identity: range1989845
display id: range1989845
version: 1
direction: direction/forward
top level: BBa_K105031
start: 22
end: 22
orientation: inline

TATA-box
type: SequenceAnnotation
persistent identity: annotation1989843
display id: annotation1989843
version: 1
top level: BBa_K105031
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K105031/annotation1989843/range1989843/1

https://synbiohub.org/public/igem/BBa_K105031/annotation1989843/range1989843/1
type: Range
persistent identity: range1989843
display id: range1989843
version: 1
direction: direction/forward
top level: BBa_K105031
start: 17
end: 23
orientation: inline

BBa_I766200
type: ComponentDefinition
persistent identity: BBa I766200
display id: BBa_I766200
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pSte2
creator: Nili Sommovilla
created: 2007-10-21T11:00:00Z
modified: 2015-08-31T04:08:09Z
discontinued: false
dominant: false
group u list: _155_
m user id: 0
owner id: 1931
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: endogenous promoter of Ste2
mutable notes: none.
mutable provenance: from yeast genome
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I766200_sequence/1

BBa_K105030
type: ComponentDefinition
persistent identity: BBa K105030
display id: BBa_K105030
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: cyc28 minimal promoter
creator: Michael Wild
created: 2008-10-25T11:00:00Z
modified: 2015-05-08T01:08:52Z
discontinued: false
dominant: false
experience: experience/None
group u list: _253_
m user id: 0
owner id: 3313
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This is a derivate of BBa_K105027. Due to a point mutation in the TATA box region the efficiency of this minimal promoter is only 28 % of the natural yeast CYC1 promoter.
mutable notes: This BioBrick contains the TATA-box with one point mutation and most of the 5'UTR (-139 to -36, relative to the translational start) of the natural CYC1 promoter. A Kozak sequence is not included and has thus to be added! Even if there isn't any need for this BioBrick to be fusible to other protein domains, we stayed with our design.
mutable provenance: Amplification and Mutation from the plasmid pAH3
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: A -> T
sequence annotation: TATA-box
sequence: https://synbiohub.org/public/igem/BBa_K105030_sequence/1

A -> T
type: SequenceAnnotation
persistent identity: annotation1989840
display id: annotation1989840
version: 1
top level: BBa_K105030
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_K105030/annotation1989840/range1989840/1

https://synbiohub.org/public/igem/BBa_K105030/annotation1989840/range1989840/1
type: Range
persistent identity: range1989840
display id: range1989840
version: 1
direction: direction/forward
top level: BBa_K105030
start: 22
end: 22
orientation: inline

TATA-box
type: SequenceAnnotation
persistent identity: annotation1989839
display id: annotation1989839
version: 1
top level: BBa_K105030
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K105030/annotation1989839/range1989839/1

https://synbiohub.org/public/igem/BBa_K105030/annotation1989839/range1989839/1
type: Range
persistent identity: range1989839
display id: range1989839
version: 1
direction: direction/forward
top level: BBa_K105030
start: 17
end: 23
orientation: inline

BBa_I766557
type: ComponentDefinition
persistent identity: BBa I766557
display id: BBa_I766557
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pSte5 (Weak) Promoter
creator: Nili Sommovilla
created: 2007-08-15T11:00:00Z
modified: 2015-08-31T04:08:10Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _155_
m user id: 0
owner id: 1931
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: low expression, constitutive promoter in yeast
mutable notes: none
mutable provenance: endogenous promoter for Sterile 5
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I766557_sequence/1

BBa_K110004
type: ComponentDefinition
persistent identity: BBa K110004
display id: BBa_K110004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Alpha-Cell Promoter Ste3
creator: James DiCarlo
created: 2008-05-29T11:00:00Z
modified: 2015-05-08T01:09:08Z
discontinued: false
dominant: false
experience: experience/None
group u list: _201_
m user id: 0
owner id: 2669
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This promoter was isolated by taking 500bp upstream of the STE3 gene of S. cerevisiae. This promoter will be activated when alpha factor is expressed in an alpha haploid yeast cell.
mutable notes: This sequence was not edited
mutable provenance: SGD
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K110004_sequence/1

GAL1
type: ComponentDefinition
persistent identity: BBa J63006
display id: BBa_J63006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: yeast GAL1 promoter
creator: Caroline Ajo-Franklin
created: 2006-10-10T11:00:00Z
modified: 2015-08-31T01:56:26Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _97_
m user id: 0
owner id: 545
owning group id: 97
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: GAL1 promoter from S. cerevisiae
mutable notes: unchanged from genomic sequence
mutable provenance: genomic GAL1 locus
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J63006_sequence/1

BBa_K165015
type: ComponentDefinition
persistent identity: BBa K165015
display id: BBa_K165015
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pADH1 yeast constituative promoter
creator: John Szymanski
created: 2008-10-25T11:00:00Z
modified: 2015-05-08T01:10:55Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _267_
m user id: 0
owner id: 2512
owning group id: 58
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This is a low-level constitutive yeast promoter.
mutable notes: -
mutable provenance: From David Drubin in the Silver Lab at Harvard.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K165015_sequence/1

BBa_K110014
type: ComponentDefinition
persistent identity: BBa K110014
display id: BBa_K110014
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: A-Cell Promoter MFA2 (backwards)
creator: James DiCarlo
created: 2008-08-26T11:00:00Z
modified: 2015-05-08T01:09:09Z
discontinued: false
dominant: false
experience: experience/None
group u list: _201_
m user id: 0
owner id: 2669
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is the same part as BBa_K110007 except it has Prefix and Suffix regions switched
mutable notes: This was taken from SGD
mutable provenance: SGD
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K110014_sequence/1

BBa_K165016
type: ComponentDefinition
persistent identity: BBa K165016
display id: BBa_K165016
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: mCYC1 minimal yeast promoter
creator: John Szymanski
created: 2008-10-25T11:00:00Z
modified: 2015-05-08T01:10:55Z
discontinued: false
dominant: false
group u list: _267_
m user id: 0
owner id: 2512
owning group id: 58
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This minimal promoter can be used as a base for designing promoters by adding binding sites upstream.
mutable notes: -
mutable provenance: From David Drubin in the Silver Lab at Harvard
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K165016_sequence/1

BBa_J24813
type: ComponentDefinition
persistent identity: BBa J24813
display id: BBa_J24813
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: URA3 Promoter from S. cerevisiae
creator: John Szymanski
created: 2008-05-01T11:00:00Z
modified: 2015-08-31T04:08:43Z
discontinued: false
dominant: false
group u list: _58_
m user id: 0
owner id: 2512
owning group id: 58
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is the whole regulatory region from the URA3 gene coding for OMP decarboxylase in S. cerevisiae budding yeast.
mutable notes: None
mutable provenance: NCBI Sequence database: http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=Nucleotide&dopt=GenBank&val=172061
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J24813_sequence/1

BBa_K110007
type: ComponentDefinition
persistent identity: BBa K110007
display id: BBa_K110007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: A-Cell Promoter MFA2
creator: James DiCarlo
created: 2008-05-29T11:00:00Z
modified: 2015-05-08T01:09:09Z
discontinued: false
dominant: false
group u list: _201_
m user id: 0
owner id: 2669
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This promoter was isolated by taking 500bp upstream of the MFA2 gene of S. cerevisiae. This promoter will be activated when A factor is expressed in an A haploid yeast cell.
mutable notes: This gene is on the Watson strand of the entered sequence.
mutable provenance: SGD
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K110007_sequence/1

BBa_K165031
type: ComponentDefinition
persistent identity: BBa K165031
display id: BBa_K165031
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: mCYC promoter plus LexA binding sites
creator: Aaron Glieberman
created: 2008-10-28T12:00:00Z
modified: 2015-05-08T01:10:56Z
discontinued: false
dominant: false
group u list: _267_
m user id: 0
owner id: 2510
owning group id: 58
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This is a composite part containing the minimal promoter mCYC preceded by multiple consecutive binding sites for the LexA binding domain.
mutable notes: It was necessary to design primers for use in PCR to remove this part from its host plasmid and transfer it to a standard Biobrick vector
mutable provenance: Composite part was taken via PCR from a synthetic plasmid containing the sequence. The plasmid came from Lawrence Berkeley National Laboratory.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K165031_sequence/1

pTEF
type: ComponentDefinition
persistent identity: BBa K124001
display id: BBa_K124001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Yeast TEF2 Constitutive Promoter
creator: John Szymanski
created: 2008-06-30T11:00:00Z
modified: 2015-05-08T01:09:44Z
discontinued: true
dominant: false
experience: experience/None
group u list: _267_
m user id: 0
owner id: 2512
owning group id: 58
part status: Released HQ 2013
sample status: Discontinued
status: status/Deleted
bookmark: false
mutable description: This is a mid-high level constitutive promoter from the yeast gene TEF2.
mutable notes: none
mutable provenance: Mumberg, Muller, and Funk. p413 pTEF http://dx.doi.org/10.1016/0378-1119(95)00037-7
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K124001_sequence/1

BBa_K105027
type: ComponentDefinition
persistent identity: BBa K105027
display id: BBa_K105027
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: cyc100 minimal promoter
creator: Michael Wild
created: 2008-10-25T11:00:00Z
modified: 2015-05-08T01:08:52Z
discontinued: false
dominant: false
experience: experience/None
group u list: _253_
m user id: 0
owner id: 3313
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This is the core of the CYC1 promoter of ''S. cerevisiae''. This promoter element allows basal transcription. The transcription signal can be modulated by adding operator sites upstream of this BioBrick. In that way activators or repressors can act on the transcription machinery. <br<
mutable notes: This BioBrick contains the TATA-box and most of the 5'UTR (-139 to -36, relative to the translational start)of the natural CYC1 promoter. A Kozak sequence is not included and has thus to be added!
mutable provenance: Amplification from the plasmid pAH3.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: TATA box
sequence: https://synbiohub.org/public/igem/BBa_K105027_sequence/1

TATA box
type: SequenceAnnotation
persistent identity: annotation1989774
display id: annotation1989774
version: 1
top level: BBa_K105027
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K105027/annotation1989774/range1989774/1

https://synbiohub.org/public/igem/BBa_K105027/annotation1989774/range1989774/1
type: Range
persistent identity: range1989774
display id: range1989774
version: 1
direction: direction/forward
top level: BBa_K105027
start: 17
end: 23
orientation: inline

BBa_K165000
type: ComponentDefinition
persistent identity: BBa K165000
display id: BBa_K165000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: MET 25 Promoter
creator: John Szymanski
created: 2008-10-24T11:00:00Z
modified: 2015-05-08T01:10:55Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _267_
m user id: 0
owner id: 2512
owning group id: 58
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: The is a repressable promoter for use in Saccharomyces cerevisiae. It is repressed by the presence of methionine. When unrepresssed, is has a moderate level of activity.
mutable notes: None.
mutable provenance: From the Saccharomyces cerevisiae genome, by way of Caroline Ajo-Franklin.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K165000_sequence/1

BBa_K149001
type: ComponentDefinition
persistent identity: BBa K149001
display id: BBa_K149001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Prp22 promoter
creator: Chris Euler
created: 2008-08-14T11:00:00Z
modified: 2015-05-08T01:10:44Z
discontinued: false
dominant: false
group u list: _243_
m user id: 0
owner id: 3441
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Ubiquitous strong promoter.
mutable notes: None
mutable provenance: Simon St. Pierre extracted from S. Cervisiae genome.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K149001_sequence/1

BBa_I766555
type: ComponentDefinition
persistent identity: BBa I766555
display id: BBa_I766555
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pCyc (Medium) Promoter
creator: Nili Sommovilla
created: 2007-08-15T11:00:00Z
modified: 2015-08-31T04:08:10Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _155_
m user id: 0
owner id: 1931
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Mid-expression level constitutive promoter in yeast
mutable notes: none
mutable provenance: Endogenous promoter for Cytochrome c
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I766555_sequence/1

BBa_K586000
type: ComponentDefinition
persistent identity: BBa K586000
display id: BBa_K586000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Cup-1 Heavy metal sensor
creator: Chris Thompson
created: 2011-06-21T11:00:00Z
modified: 2015-05-08T01:12:47Z
discontinued: false
dominant: false
experience: experience/None
group u list: _757_
m user id: 0
owner id: 9336
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Cup-1 is a regulatory promoter that senses heavy metals such as copper, arsenic, cadmium, antimony, mercury, chromium, and selenium.
mutable notes: None
mutable provenance: Comes from genomic DNA of saccharomyces cerevisiae.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: Cup-1
sequence: https://synbiohub.org/public/igem/BBa_K586000_sequence/1

Cup-1
type: SequenceAnnotation
persistent identity: annotation2122521
display id: annotation2122521
version: 1
top level: BBa_K586000
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K586000/annotation2122521/range2122521/1

https://synbiohub.org/public/igem/BBa_K586000/annotation2122521/range2122521/1
type: Range
persistent identity: range2122521
display id: range2122521
version: 1
direction: direction/forward
top level: BBa_K586000
start: 1
end: 186
orientation: inline