Composite part that was taken via PCR from a synthetic plasmid containing the sequence. The plasmid came from Lawrence Berkeley National Laboratory.
This is a composite part containing the minimal promoter mCYC preceded by multiple consecutive binding sites for the Gli1 binding domain. This part contains the sequences for BBa_K165012 and BBa_K165016 but also has some additional DNA.
It was necessary to design primers for use in PCR to remove this part from its host plasmid and transfer it to a standard Biobrick vector.
Constructed in our lab with parts from Caroline Ajo-Franklin and David Drubin.
The Gli1 and Zif268-HIV transcription factor binding sites on the mCYC minimal promoter (in that order.) Transcription regulation comes from Gli1 and Zif268-HIV binding transcription factors.
Zif268-HIV binding sites + MET25 constitutive yeast promoter
This is the same sequence as STE2 promotor(BBa_K110008), but with suffix and prefix switched
Regulatory region spanning 680 bp upstream of the start codon of the GPD1 gene in yeast. Very strong constitutive promoter.
Comes from genomic DNA of saccharomyces cerevisiae.
Cup-1 is a regulatory promoter that senses heavy metals such as copper, arsenic, cadmium, antimony, mercury, chromium, and selenium.
Simon St. Pierre extracted from S. Cervisiae genome.
From David Drubin in the Silver Lab at Harvard.
This is a low-level constitutive yeast promoter.
Gli1 binding sites + ADH1 constitutive yeast promoter
The Gli1 transcription factor binding sites on the ADH1 constitutive promoter. Transcription regulation should be constitutive plus regulated by YY1-binding transcription factors.
Mid-expression level constitutive promoter in yeast
From the Saccharomyces cerevisiae genome, via Caroline Ajo-Franklin.
This is the GAL1 inducible promoter for use in Saccharomyces cerevisiae. It is repressed in the presence of glucose and induced in the presence of galactose. Raffinose is a neutral sugar source the neither induces nor represses the promoter. pGAL1 normally has an "all-or-nothing" response to galactose, but Christina Smolke has shown that knockout of the Gal2 gene gives pGAL1 a tunable, linear response to galactose.
This promoter was isolated by taking 500bp upstream of the MF(ALPHA)2 gene of S. cerevisiae. This promoter will be activated when alpha factor is expressed in an alpha haploid yeast cell.
This gene is on the crick strand of the entered sequence.
Endogenous promoter for Alcohol Dehydrogenase
Strong Expression level Constitutive promoter in yeast
CMV promoter with inserted lac operator sites. Lac operator from POPI3Cat
Zif268-HIV bs + LexA bs + mCYC promoter
The Zif268-HIV and LexA transcription factor binding sites on the mCYC minimal promoter (in that order.) Transcription regulation comes from Zif268-HIV and LexA-binding transcription factors.
mCYC promoter plus Zif268-HIV binding sites
This is a composite part containing the minimal promoter mCYC preceded by multiple consecutive binding sites for the Zif268-HIV binding domain. It contains the sequences of parts BBa_K165011 and BBa_K165016 but also contains additional DNA.
endogenous promoter for Fig1 (Factor Induced Gene 1)
This promoter was isolated by taking 500bp upstream of the MFA2 gene of S. cerevisiae. This promoter will be activated when A factor is expressed in an A haploid yeast cell.
This gene is on the Watson strand of the entered sequence.
This promoter was isolated by taking 500bp upstream of the STE3 gene of S. cerevisiae. This promoter will be activated when alpha factor is expressed in an alpha haploid yeast cell.
This promoter was isolated by taking 500bp upstream of the MFA1 gene of S. cerevisiae. This promoter will be activated when A factor is expressed in an A haploid yeast cell.
This promotor is on the crick strand of the entered sequence.
Rick Carrick, Ambhi Ganesan, Alex McMillan
Amplification and Mutation from the plasmid pAH3
This is a derivate of BBa_K105027. Due to a point mutation in the TATA box region the efficiency of this minimal promoter is only 43 % of the natural yeast CYC1 promoter.
This BioBrick contains the TATA-box with one point mutation and most of the 5'UTR (-139 to -36, relative to the translational start) of the natural CYC1 promoter. A Kozak sequence is not included and has thus to be added! Even if there isn't any need for this BioBrick to be fusible to other protein domains, we stayed with our design.
a constitutive expression promoter or use in mammalian cells. Ribosome binding site is included.
From David Drubin in the Silver Lab at Harvard
This minimal promoter can be used as a base for designing promoters by adding binding sites upstream.
Composite part was taken via PCR from a synthetic plasmid containing the sequence. The plasmid came from Lawrence Berkeley National Laboratory.
This is a composite part containing the minimal promoter mCYC preceded by multiple consecutive binding sites for the LexA binding domain.
It was necessary to design primers for use in PCR to remove this part from its host plasmid and transfer it to a standard Biobrick vector
Yeast CLB1 promoter region, G2/M cell cycle specific
Yeast consensus sequence from SGD chrVII:703140..703639
Description of CLB1 (from SGD): B-type cyclin involved in cell cycle progression; activates Cdc28p to promote the transition from G2 to M phase; accumulates during G2 and M, then targeted via a destruction box motif for ubiquitin-mediated degradation by the proteasome. More information found here: http://db.yeastgenome.org/cgi-bin/locus.pl?locus=CLB1
Part contains 500 bases upstream from the CLB1 ORF. Is NOT a minimal promoter, and has not been tested for composability.
NCBI Sequence database: http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=Nucleotide&dopt=GenBank&val=172061
This is the whole regulatory region from the URA3 gene coding for OMP decarboxylase in S. cerevisiae budding yeast.
From the Saccharomyces cerevisiae genome, by way of Caroline Ajo-Franklin.
The is a repressable promoter for use in Saccharomyces cerevisiae. It is repressed by the presence of methionine. When unrepresssed, is has a moderate level of activity.
This part was obtained from yeast genomic DNA.
This is the 1500 bp upstream of the yeast PGK1 coding region. This is a putative constitutive promoter.
Source: Saccharomyces cerevisiae genomic DNA.
The part codes for the human Oct-4 promoter, which regulates expression of the Oct-4 gene. The Oct-4 gene is essential for pluripotency in cells and is downregulated once differentiation occurs. Sequence information is from GenBank (NM_002701) and shows the cis-regulatory mRNA that controls transcriptional activation of the human Oct-4 promoter.
From the yeast genome using promoter definition from: Mumberg, Muller, and Funk: http://dx.doi.org/10.1016/0378-1119(95)00037-7
This is a high level constitutive promoter from the yeast gene TEF2.
This is a derivate of BBa_K105027. Due to a point mutation in the TATA box region the efficiency of this minimal promoter is only 70 % of the natural yeast CYC1 promoter.
This is a derivate of BBa_K105027. Due to a point mutation in the TATA box region the efficiency of this minimal promoter is only 28 % of the natural yeast CYC1 promoter.
Zif268-HIV binding sites + TEF constitutive yeast promoter
This is a mid-high level constitutive promoter from the yeast gene TEF2.
Zif268-HIV binding sites + ADH1 yeast promoter
The Zif268-HIV transcription factor binding sites on the ADH1 constitutive promoter. Transcription regulation should be constitutive plus regulated by Zif268-HIV-binding transcription factors.
This promoter was isolated by taking 500bp upstream of the MF(ALPHA)1 gene of S. cerevisiae. This promoter will be activated when alpha factor is expressed in an alpha haploid yeast cell.
Made in our lab with parts from Caroline Ajo-Franklin and David Drubin.
The YY1 transcription factor binding sites on the mCYC minimal promoter. All transcription regulation comes from YY1-binding transcription factors.
Gli1 binding sites + TEF constitutive yeast promoter
The Gli1 transcription factor binding sites on the TEF2 constitutive promoter. Transcription regulation should be constitutive plus regulated by Gli1-binding transcription factors.
low expression, constitutive promoter in yeast
Gli1 binding sites + MET25 inducible yeast promoter
Using this promoter allows for the constant expression of a reporter gene or a selectable antibiotic.
This part was obtained from members of the Weiss Lab.
From Mumberg, Muller, and Funk p413 pCYC. See ref. above
The promoter from the yeast gene CYC1. Activity characterized here: http://dx.doi.org/10.1016/0378-1119(95)00037-7.
This is the same part as BBa_K110007 except it has Prefix and Suffix regions switched
The Gli1 and LexA transcription factor binding sites on the mCYC minimal promoter (in that order.) Transcription regulation comes from Gli1 and LexA-binding transcription factors.
This is the same sequence as MFA1 promotor(BBa_K110008), but with suffix and prefix switched
This is a derivate of BBa_K105027. Due to a point mutation in the TATA box region the efficiency of this minimal promoter is only 16 % of the natural yeast CYC1 promoter.
This is the core of the CYC1 promoter of ''S. cerevisiae''. This promoter element allows basal transcription. The transcription signal can be modulated by adding operator sites upstream of this BioBrick. In that way activators or repressors can act on the transcription machinery. <br<
This BioBrick contains the TATA-box and most of the 5'UTR (-139 to -36, relative to the translational start)of the natural CYC1 promoter. A Kozak sequence is not included and has thus to be added!
Conversion of the iGEM parts registry to SBOL2.1