//rnap/eukaryote
type: Collection
persistent identity: rnap eukaryote
display id: rnap_eukaryote
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
member: BBa_K076017
member: BBa_J05218
member: BBa_I712004
member: BBa_I756019
member: BBa_I756016
member: BBa_J05216
member: BBa_J05215
member: BBa_I756020
member: BBa_I756015
member: BBa_I756021
member: BBa_J05217
member: BBa_I756017
member: BBa_I756018
member: Oct-4 prom
member: //rnap/eukaryote/pichia
member: //rnap/eukaryote/yeast

//rnap/eukaryote/yeast
type: Collection
persistent identity: rnap eukaryote yeast
display id: rnap_eukaryote_yeast
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
member: BBa_K165000
member: BBa_K110016
member: BBa_K165030
member: BBa_I766214
member: BBa_K110007
member: BBa_K165035
member: BBa_K110009
member: BBa_K124002
member: BBa_I766200
member: BBa_I766556
member: BBa_K110004
member: BBa_M31201
member: BBa_K105031
member: BBa_I766557
member: P0310
member: BBa_K165041
member: BBa_K105030
member: BBa_K110005
member: pTEF
member: BBa_K124000
member: BBa_I766558
member: BBa_K165042
member: GAL1
member: BBa_K106699
member: BBa_K110006
member: BBa_K165038
member: BBa_K165043
member: BBa_K122000
member: BBa_K165039
member: BBa_K165031
member: BBa_K149001
member: BBa_K165001
member: BBa_K165015
member: BBa_K110008
member: BBa_K165036
member: BBa_K105027
member: BBa_K165032
member: BBa_K165040
member: BBa_K165016
member: BBa_K105028
member: BBa_K110014
member: BBa_K165033
member: BBa_J24813
member: BBa_K586000
member: BBa_I766555
member: BBa_K110015
member: BBa_K165034
member: BBa_K105029

//rnap/eukaryote/pichia
type: Collection
persistent identity: rnap eukaryote pichia
display id: rnap_eukaryote_pichia
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
member: BBa_I764001

BBa_K165032
type: ComponentDefinition
persistent identity: BBa K165032
display id: BBa_K165032
version: 1
was generated by: iGEM to SBOL conversion
description: mCYC promoter plus Gli1 binding sites
created: 2008-10-28T12:00:00Z
modified: 2015-05-08T01:10:56Z
mutable provenance: Composite part that was taken via PCR from a synthetic plasmid containing the sequence. The plasmid came from Lawrence Berkeley National Laboratory.
owned by: james
owned by: myers
mutable description: This is a composite part containing the minimal promoter mCYC preceded by multiple consecutive binding sites for the Gli1 binding domain. This part contains the sequences for BBa_K165012 and BBa_K165016 but also has some additional DNA.
discontinued: false
dominant: false
group u list: _267_
m user id: 0
owner id: 2510
owning group id: 58
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: It was necessary to design primers for use in PCR to remove this part from its host plasmid and transfer it to a standard Biobrick vector.
star: false
creator: Aaron Glieberman
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K165032_sequence/1

BBa_K165035
type: ComponentDefinition
persistent identity: BBa K165035
display id: BBa_K165035
version: 1
was generated by: iGEM to SBOL conversion
description: Gli1 bs + Zif268-HIV bs + mCYC promoter
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:10:56Z
mutable provenance: Constructed in our lab with parts from Caroline Ajo-Franklin and David Drubin.
owned by: james
owned by: myers
mutable description: The Gli1 and Zif268-HIV transcription factor binding sites on the mCYC minimal promoter (in that order.) Transcription regulation comes from Gli1 and Zif268-HIV binding transcription factors.
discontinued: false
dominant: false
group u list: _267_
m user id: 0
owner id: 2512
owning group id: 58
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: -
star: false
creator: John Szymanski
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
component: BBa_K165030
component: BBa_K165012
sequence annotation: BBa_K165012
sequence annotation: BBa_K165030
sequence: https://synbiohub.org/public/igem/BBa_K165035_sequence/1

BBa_K165030
type: Component
persistent identity: component1995497
display id: component1995497
version: 1
top level: BBa_K165035
access: public
definition: BBa_K165030

BBa_K165012
type: Component
persistent identity: component1995495
display id: component1995495
version: 1
top level: BBa_K165035
access: public
definition: BBa_K165012

BBa_K165012
type: SequenceAnnotation
persistent identity: annotation1995495
display id: annotation1995495
version: 1
top level: BBa_K165035
location: https://synbiohub.org/public/igem/BBa_K165035/annotation1995495/range1995495/1
component: BBa_K165012

https://synbiohub.org/public/igem/BBa_K165035/annotation1995495/range1995495/1
type: Range
persistent identity: range1995495
display id: range1995495
version: 1
top level: BBa_K165035
direction: direction/reverse
start: 1
end: 127
orientation: inline

BBa_K165030
type: SequenceAnnotation
persistent identity: annotation1995497
display id: annotation1995497
version: 1
top level: BBa_K165035
location: https://synbiohub.org/public/igem/BBa_K165035/annotation1995497/range1995497/1
component: BBa_K165030

https://synbiohub.org/public/igem/BBa_K165035/annotation1995497/range1995497/1
type: Range
persistent identity: range1995497
display id: range1995497
version: 1
top level: BBa_K165035
direction: direction/reverse
start: 136
end: 442
orientation: inline

BBa_K165043
type: ComponentDefinition
persistent identity: BBa K165043
display id: BBa_K165043
version: 1
was generated by: iGEM to SBOL conversion
description: Zif268-HIV binding sites + MET25 constitutive yeast promoter
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:10:56Z
mutable provenance: -
owned by: james
owned by: myers
mutable description: -
discontinued: false
dominant: false
group u list: _267_
m user id: 0
owner id: 2512
owning group id: 58
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: -
star: true
creator: John Szymanski
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
component: BBa_K165011
component: BBa_K165000
sequence annotation: BBa_K165011
sequence annotation: BBa_K165000
sequence: https://synbiohub.org/public/igem/BBa_K165043_sequence/1

BBa_K165011
type: Component
persistent identity: component1996569
display id: component1996569
version: 1
top level: BBa_K165043
access: public
definition: BBa_K165011

BBa_K165000
type: Component
persistent identity: component1996570
display id: component1996570
version: 1
top level: BBa_K165043
access: public
definition: BBa_K165000

BBa_K165011
type: SequenceAnnotation
persistent identity: annotation1996569
display id: annotation1996569
version: 1
top level: BBa_K165043
location: https://synbiohub.org/public/igem/BBa_K165043/annotation1996569/range1996569/1
component: BBa_K165011

https://synbiohub.org/public/igem/BBa_K165043/annotation1996569/range1996569/1
type: Range
persistent identity: range1996569
display id: range1996569
version: 1
top level: BBa_K165043
direction: direction/reverse
start: 1
end: 46
orientation: inline

BBa_K165000
type: SequenceAnnotation
persistent identity: annotation1996570
display id: annotation1996570
version: 1
top level: BBa_K165043
location: https://synbiohub.org/public/igem/BBa_K165043/annotation1996570/range1996570/1
component: BBa_K165000

https://synbiohub.org/public/igem/BBa_K165043/annotation1996570/range1996570/1
type: Range
persistent identity: range1996570
display id: range1996570
version: 1
top level: BBa_K165043
direction: direction/reverse
start: 55
end: 441
orientation: inline

BBa_K110016
type: ComponentDefinition
persistent identity: BBa K110016
display id: BBa_K110016
version: 1
was generated by: iGEM to SBOL conversion
description: A-Cell Promoter STE2 (backwards)
created: 2008-08-26T11:00:00Z
modified: 2015-05-08T01:09:09Z
mutable provenance: sgd
owned by: james
owned by: myers
mutable description: This is the same sequence as STE2 promotor(BBa_K110008), but with suffix and prefix switched
discontinued: false
dominant: false
experience: experience/None
group u list: _201_
m user id: 0
owner id: 2669
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: This is directly from the DNA sequence
star: true
creator: James DiCarlo
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: STE12
sequence annotation: STE2 Promoter
sequence annotation: MATalpha2
sequence annotation: MCM1
sequence: https://synbiohub.org/public/igem/BBa_K110016_sequence/1

STE12
type: SequenceAnnotation
persistent identity: annotation1981095
display id: annotation1981095
version: 1
top level: BBa_K110016
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K110016/annotation1981095/range1981095/1

https://synbiohub.org/public/igem/BBa_K110016/annotation1981095/range1981095/1
type: Range
persistent identity: range1981095
display id: range1981095
version: 1
top level: BBa_K110016
direction: direction/forward
start: 304
end: 311
orientation: inline

STE2 Promoter
type: SequenceAnnotation
persistent identity: annotation1981094
display id: annotation1981094
version: 1
top level: BBa_K110016
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K110016/annotation1981094/range1981094/1

https://synbiohub.org/public/igem/BBa_K110016/annotation1981094/range1981094/1
type: Range
persistent identity: range1981094
display id: range1981094
version: 1
top level: BBa_K110016
direction: direction/forward
start: 1
end: 500
orientation: inline

MATalpha2
type: SequenceAnnotation
persistent identity: annotation1981093
display id: annotation1981093
version: 1
top level: BBa_K110016
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K110016/annotation1981093/range1981093/1

https://synbiohub.org/public/igem/BBa_K110016/annotation1981093/range1981093/1
type: Range
persistent identity: range1981093
display id: range1981093
version: 1
top level: BBa_K110016
direction: direction/forward
start: 295
end: 302
orientation: inline

MATalpha2
type: SequenceAnnotation
persistent identity: annotation1981091
display id: annotation1981091
version: 1
top level: BBa_K110016
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K110016/annotation1981091/range1981091/1

https://synbiohub.org/public/igem/BBa_K110016/annotation1981091/range1981091/1
type: Range
persistent identity: range1981091
display id: range1981091
version: 1
top level: BBa_K110016
direction: direction/forward
start: 273
end: 282
orientation: inline

MCM1
type: SequenceAnnotation
persistent identity: annotation1981092
display id: annotation1981092
version: 1
top level: BBa_K110016
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K110016/annotation1981092/range1981092/1

https://synbiohub.org/public/igem/BBa_K110016/annotation1981092/range1981092/1
type: Range
persistent identity: range1981092
display id: range1981092
version: 1
top level: BBa_K110016
direction: direction/forward
start: 284
end: 293
orientation: inline

BBa_K124002
type: ComponentDefinition
persistent identity: BBa K124002
display id: BBa_K124002
version: 1
was generated by: iGEM to SBOL conversion
description: Yeast GPD (TDH3) Promoter
created: 2008-06-30T11:00:00Z
modified: 2015-05-08T01:09:44Z
mutable provenance: Mumberg, Muller, and Funk. p413 pGPD http://dx.doi.org/10.1016/0378-1119(95)00037-7
owned by: james
owned by: myers
mutable description: Regulatory region spanning 680 bp upstream of the start codon of the GPD1 gene in yeast. Very strong constitutive promoter.
discontinued: false
dominant: false
experience: experience/Works
group u list: _221_
m user id: 0
owner id: 2512
owning group id: 58
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: none
star: false
creator: John Szymanski
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K124002_sequence/1

GAL1
type: ComponentDefinition
persistent identity: BBa J63006
display id: BBa_J63006
version: 1
was generated by: iGEM to SBOL conversion
description: yeast GAL1 promoter
created: 2006-10-10T11:00:00Z
modified: 2015-08-31T01:56:26Z
mutable provenance: genomic GAL1 locus
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: GAL1 promoter from S. cerevisiae
discontinued: false
dominant: true
experience: experience/Works
group u list: _97_
m user id: 0
owner id: 545
owning group id: 97
sample status: In stock
status: status/Available
bookmark: false
mutable notes: unchanged from genomic sequence
star: true
creator: Caroline Ajo-Franklin
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J63006_sequence/1

BBa_K586000
type: ComponentDefinition
persistent identity: BBa K586000
display id: BBa_K586000
version: 1
was generated by: iGEM to SBOL conversion
description: Cup-1 Heavy metal sensor
created: 2011-06-21T11:00:00Z
modified: 2015-05-08T01:12:47Z
mutable provenance: Comes from genomic DNA of saccharomyces cerevisiae.
owned by: james
owned by: myers
mutable description: Cup-1 is a regulatory promoter that senses heavy metals such as copper, arsenic, cadmium, antimony, mercury, chromium, and selenium.
discontinued: false
dominant: false
experience: experience/None
group u list: _757_
m user id: 0
owner id: 9336
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: None
star: false
creator: Chris Thompson
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: Cup-1
sequence: https://synbiohub.org/public/igem/BBa_K586000_sequence/1

Cup-1
type: SequenceAnnotation
persistent identity: annotation2122521
display id: annotation2122521
version: 1
top level: BBa_K586000
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K586000/annotation2122521/range2122521/1

https://synbiohub.org/public/igem/BBa_K586000/annotation2122521/range2122521/1
type: Range
persistent identity: range2122521
display id: range2122521
version: 1
top level: BBa_K586000
direction: direction/forward
start: 1
end: 186
orientation: inline

BBa_K149001
type: ComponentDefinition
persistent identity: BBa K149001
display id: BBa_K149001
version: 1
was generated by: iGEM to SBOL conversion
description: Prp22 promoter
created: 2008-08-14T11:00:00Z
modified: 2015-05-08T01:10:44Z
mutable provenance: Simon St. Pierre extracted from S. Cervisiae genome.
owned by: myers
owned by: james
mutable description: Ubiquitous strong promoter.
discontinued: false
dominant: false
group u list: _243_
m user id: 0
owner id: 3441
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: None
star: false
creator: Chris Euler
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K149001_sequence/1

BBa_K165015
type: ComponentDefinition
persistent identity: BBa K165015
display id: BBa_K165015
version: 1
was generated by: iGEM to SBOL conversion
description: pADH1 yeast constituative promoter
created: 2008-10-25T11:00:00Z
modified: 2015-05-08T01:10:55Z
mutable provenance: From David Drubin in the Silver Lab at Harvard.
owned by: james
owned by: myers
mutable description: This is a low-level constitutive yeast promoter.
discontinued: false
dominant: false
experience: experience/Works
group u list: _267_
m user id: 0
owner id: 2512
owning group id: 58
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: -
star: false
creator: John Szymanski
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K165015_sequence/1

BBa_K165038
type: ComponentDefinition
persistent identity: BBa K165038
display id: BBa_K165038
version: 1
was generated by: iGEM to SBOL conversion
description: Gli1 binding sites + ADH1 constitutive yeast promoter
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:10:56Z
mutable provenance: Constructed in our lab with parts from Caroline Ajo-Franklin and David Drubin.
owned by: james
owned by: myers
mutable description: The Gli1 transcription factor binding sites on the ADH1 constitutive promoter. Transcription regulation should be constitutive plus regulated by YY1-binding transcription factors.
discontinued: false
dominant: false
group u list: _267_
m user id: 0
owner id: 2512
owning group id: 58
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: -
star: false
creator: John Szymanski
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
component: BBa_K165015
component: BBa_K165012
sequence annotation: BBa_K165015
sequence annotation: BBa_K165012
sequence: https://synbiohub.org/public/igem/BBa_K165038_sequence/1

BBa_K165015
type: Component
persistent identity: component1995513
display id: component1995513
version: 1
top level: BBa_K165038
access: public
definition: BBa_K165015

BBa_K165012
type: Component
persistent identity: component1995512
display id: component1995512
version: 1
top level: BBa_K165038
access: public
definition: BBa_K165012

BBa_K165015
type: SequenceAnnotation
persistent identity: annotation1995513
display id: annotation1995513
version: 1
top level: BBa_K165038
location: https://synbiohub.org/public/igem/BBa_K165038/annotation1995513/range1995513/1
component: BBa_K165015

https://synbiohub.org/public/igem/BBa_K165038/annotation1995513/range1995513/1
type: Range
persistent identity: range1995513
display id: range1995513
version: 1
top level: BBa_K165038
direction: direction/reverse
start: 136
end: 1580
orientation: inline

BBa_K165012
type: SequenceAnnotation
persistent identity: annotation1995512
display id: annotation1995512
version: 1
top level: BBa_K165038
location: https://synbiohub.org/public/igem/BBa_K165038/annotation1995512/range1995512/1
component: BBa_K165012

https://synbiohub.org/public/igem/BBa_K165038/annotation1995512/range1995512/1
type: Range
persistent identity: range1995512
display id: range1995512
version: 1
top level: BBa_K165038
direction: direction/reverse
start: 1
end: 127
orientation: inline

BBa_I766555
type: ComponentDefinition
persistent identity: BBa I766555
display id: BBa_I766555
version: 1
was generated by: iGEM to SBOL conversion
description: pCyc (Medium) Promoter
created: 2007-08-15T11:00:00Z
modified: 2015-08-31T04:08:10Z
mutable provenance: Endogenous promoter for Cytochrome c
owned by: james
owned by: myers
mutable description: Mid-expression level constitutive promoter in yeast
discontinued: false
dominant: false
experience: experience/Works
group u list: _155_
m user id: 0
owner id: 1931
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: none
star: false
creator: Nili Sommovilla
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I766555_sequence/1

BBa_K165001
type: ComponentDefinition
persistent identity: BBa K165001
display id: BBa_K165001
version: 1
was generated by: iGEM to SBOL conversion
description: pGAL1+ w/XhoI sites
created: 2008-10-25T11:00:00Z
modified: 2015-05-08T01:10:55Z
mutable provenance: From the Saccharomyces cerevisiae genome, via Caroline Ajo-Franklin.
owned by: james
owned by: myers
mutable description: This is the GAL1 inducible promoter for use in Saccharomyces cerevisiae. It is repressed in the presence of glucose and induced in the presence of galactose. Raffinose is a neutral sugar source the neither induces nor represses the promoter. pGAL1 normally has an "all-or-nothing" response to galactose, but Christina Smolke has shown that knockout of the Gal2 gene gives pGAL1 a tunable, linear response to galactose.
discontinued: false
dominant: false
group u list: _267_
m user id: 0
owner id: 2512
owning group id: 58
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: none
star: true
creator: John Szymanski
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K165001_sequence/1

BBa_K110005
type: ComponentDefinition
persistent identity: BBa K110005
display id: BBa_K110005
version: 1
was generated by: iGEM to SBOL conversion
description: Alpha-Cell Promoter MF(ALPHA)2
created: 2008-05-29T11:00:00Z
modified: 2015-05-08T01:09:08Z
mutable provenance: SGD
owned by: james
owned by: myers
mutable description: This promoter was isolated by taking 500bp upstream of the MF(ALPHA)2 gene of S. cerevisiae. This promoter will be activated when alpha factor is expressed in an alpha haploid yeast cell.
discontinued: false
dominant: false
experience: experience/None
group u list: _201_
m user id: 0
owner id: 2669
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: This gene is on the crick strand of the entered sequence.
star: true
creator: James DiCarlo
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: MF(alpha)2
sequence annotation: MCM1
sequence: https://synbiohub.org/public/igem/BBa_K110005_sequence/1

MF(alpha)2
type: SequenceAnnotation
persistent identity: annotation1997686
display id: annotation1997686
version: 1
top level: BBa_K110005
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K110005/annotation1997686/range1997686/1

https://synbiohub.org/public/igem/BBa_K110005/annotation1997686/range1997686/1
type: Range
persistent identity: range1997686
display id: range1997686
version: 1
top level: BBa_K110005
direction: direction/forward
start: 1
end: 500
orientation: inline

MCM1
type: SequenceAnnotation
persistent identity: annotation1997684
display id: annotation1997684
version: 1
top level: BBa_K110005
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K110005/annotation1997684/range1997684/1

https://synbiohub.org/public/igem/BBa_K110005/annotation1997684/range1997684/1
type: Range
persistent identity: range1997684
display id: range1997684
version: 1
top level: BBa_K110005
direction: direction/forward
start: 184
end: 209
orientation: inline

BBa_I766556
type: ComponentDefinition
persistent identity: BBa I766556
display id: BBa_I766556
version: 1
was generated by: iGEM to SBOL conversion
description: pAdh (Strong) Promoter
created: 2007-08-15T11:00:00Z
modified: 2015-08-31T04:08:10Z
mutable provenance: Endogenous promoter for Alcohol Dehydrogenase
owned by: james
owned by: myers
mutable description: Strong Expression level Constitutive promoter in yeast
discontinued: false
dominant: false
experience: experience/Works
group u list: _155_
m user id: 0
owner id: 1931
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: none
star: false
creator: Nili Sommovilla
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I766556_sequence/1

BBa_I756015
type: ComponentDefinition
persistent identity: BBa I756015
display id: BBa_I756015
version: 1
was generated by: iGEM to SBOL conversion
description: CMV Promoter with lac operator sites
creator: Jack
created: 2007-08-15T11:00:00Z
modified: 2015-08-31T04:08:06Z
discontinued: false
dominant: false
experience: experience/None
group u list: _108_
m user id: 0
owner id: 2067
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: CMV Promoter with lac operator sites.
mutable notes: CMV promoter with inserted lac operator sites. Lac operator from POPI3Cat
mutable provenance: Promoter
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: Lac operator
sequence: https://synbiohub.org/public/igem/BBa_I756015_sequence/1

Lac operator
type: SequenceAnnotation
persistent identity: annotation1941580
display id: annotation1941580
version: 1
top level: BBa_I756015
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I756015/annotation1941580/range1941580/1

https://synbiohub.org/public/igem/BBa_I756015/annotation1941580/range1941580/1
type: Range
persistent identity: range1941580
display id: range1941580
version: 1
direction: direction/forward
top level: BBa_I756015
start: 513
end: 619
orientation: inline

BBa_K165034
type: ComponentDefinition
persistent identity: BBa K165034
display id: BBa_K165034
version: 1
was generated by: iGEM to SBOL conversion
description: Zif268-HIV bs + LexA bs + mCYC promoter
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:10:56Z
mutable provenance: Constructed in our lab with parts from Caroline Ajo-Franklin and David Drubin.
owned by: james
owned by: myers
mutable description: The Zif268-HIV and LexA transcription factor binding sites on the mCYC minimal promoter (in that order.) Transcription regulation comes from Zif268-HIV and LexA-binding transcription factors.
discontinued: false
dominant: false
group u list: _267_
m user id: 0
owner id: 2512
owning group id: 58
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: -
star: true
creator: John Szymanski
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
component: BBa_K165011
component: BBa_K165031
sequence annotation: BBa_K165031
sequence annotation: BBa_K165011
sequence: https://synbiohub.org/public/igem/BBa_K165034_sequence/1

BBa_K165011
type: Component
persistent identity: component1995464
display id: component1995464
version: 1
top level: BBa_K165034
access: public
definition: BBa_K165011

BBa_K165031
type: Component
persistent identity: component1995465
display id: component1995465
version: 1
top level: BBa_K165034
access: public
definition: BBa_K165031

BBa_K165031
type: SequenceAnnotation
persistent identity: annotation1995465
display id: annotation1995465
version: 1
top level: BBa_K165034
location: https://synbiohub.org/public/igem/BBa_K165034/annotation1995465/range1995465/1
component: BBa_K165031

https://synbiohub.org/public/igem/BBa_K165034/annotation1995465/range1995465/1
type: Range
persistent identity: range1995465
display id: range1995465
version: 1
top level: BBa_K165034
direction: direction/reverse
start: 55
end: 457
orientation: inline

BBa_K165011
type: SequenceAnnotation
persistent identity: annotation1995464
display id: annotation1995464
version: 1
top level: BBa_K165034
location: https://synbiohub.org/public/igem/BBa_K165034/annotation1995464/range1995464/1
component: BBa_K165011

https://synbiohub.org/public/igem/BBa_K165034/annotation1995464/range1995464/1
type: Range
persistent identity: range1995464
display id: range1995464
version: 1
top level: BBa_K165034
direction: direction/reverse
start: 1
end: 46
orientation: inline

BBa_K165030
type: ComponentDefinition
persistent identity: BBa K165030
display id: BBa_K165030
version: 1
was generated by: iGEM to SBOL conversion
description: mCYC promoter plus Zif268-HIV binding sites
created: 2008-10-28T12:00:00Z
modified: 2015-05-08T01:10:56Z
mutable provenance: Composite part that was taken via PCR from a synthetic plasmid containing the sequence. The plasmid came from Lawrence Berkeley National Laboratory.
owned by: james
owned by: myers
mutable description: This is a composite part containing the minimal promoter mCYC preceded by multiple consecutive binding sites for the Zif268-HIV binding domain. It contains the sequences of parts BBa_K165011 and BBa_K165016 but also contains additional DNA.
discontinued: false
dominant: false
group u list: _267_
m user id: 0
owner id: 2510
owning group id: 58
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: It was necessary to design primers for use in PCR to remove this part from its host plasmid and transfer it to a standard Biobrick vector.
star: false
creator: Aaron Glieberman
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: Zif268-HIV binding sites (3)
sequence: https://synbiohub.org/public/igem/BBa_K165030_sequence/1

Zif268-HIV binding sites (3)
type: SequenceAnnotation
persistent identity: annotation1994037
display id: annotation1994037
version: 1
top level: BBa_K165030
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K165030/annotation1994037/range1994037/1

https://synbiohub.org/public/igem/BBa_K165030/annotation1994037/range1994037/1
type: Range
persistent identity: range1994037
display id: range1994037
version: 1
top level: BBa_K165030
direction: direction/forward
start: 1
end: 46
orientation: inline

BBa_I766558
type: ComponentDefinition
persistent identity: BBa I766558
display id: BBa_I766558
version: 1
was generated by: iGEM to SBOL conversion
description: pFig1 (Inducible) Promoter
created: 2007-08-15T11:00:00Z
modified: 2015-08-31T04:08:10Z
mutable provenance: endogenous promoter for Fig1 (Factor Induced Gene 1)
owned by: james
owned by: myers
mutable description: Inducible promoter in yeast
discontinued: false
dominant: false
experience: experience/None
group u list: _155_
m user id: 0
owner id: 1931
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: none
star: false
creator: Nili Sommovilla
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I766558_sequence/1

BBa_I766200
type: ComponentDefinition
persistent identity: BBa I766200
display id: BBa_I766200
version: 1
was generated by: iGEM to SBOL conversion
description: pSte2
created: 2007-10-21T11:00:00Z
modified: 2015-08-31T04:08:09Z
mutable provenance: from yeast genome
owned by: james
owned by: myers
mutable description: endogenous promoter of Ste2
discontinued: false
dominant: false
group u list: _155_
m user id: 0
owner id: 1931
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: none.
star: false
creator: Nili Sommovilla
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I766200_sequence/1

BBa_K110007
type: ComponentDefinition
persistent identity: BBa K110007
display id: BBa_K110007
version: 1
was generated by: iGEM to SBOL conversion
description: A-Cell Promoter MFA2
created: 2008-05-29T11:00:00Z
modified: 2015-05-08T01:09:09Z
mutable provenance: SGD
owned by: james
owned by: myers
mutable description: This promoter was isolated by taking 500bp upstream of the MFA2 gene of S. cerevisiae. This promoter will be activated when A factor is expressed in an A haploid yeast cell.
discontinued: false
dominant: false
group u list: _201_
m user id: 0
owner id: 2669
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: This gene is on the Watson strand of the entered sequence.
star: false
creator: James DiCarlo
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K110007_sequence/1

BBa_I756017
type: ComponentDefinition
persistent identity: BBa I756017
display id: BBa_I756017
version: 1
was generated by: iGEM to SBOL conversion
description: U6 promoter with tet operators
creator: Jack
created: 2007-08-15T11:00:00Z
modified: 2015-08-31T04:08:06Z
discontinued: false
dominant: false
group u list: _108_
m user id: 0
owner id: 2067
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: U6 promoter with tet operators
mutable notes: U6 promoter with tet operators
mutable provenance: U6 promoter with tet operators
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I756017_sequence/1

BBa_J05216
type: ComponentDefinition
persistent identity: BBa J05216
display id: BBa_J05216
version: 1
was generated by: iGEM to SBOL conversion
description: Regulator for R3-ATF6
creator: Alexander Roth
created: 2005-09-07T11:00:00Z
modified: 2015-08-31T04:08:15Z
discontinued: false
dominant: false
group u list: _34_
m user id: 0
owner id: 412
owning group id: 34
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: binding sites for R1-CREBH and R4-cMaf
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J05216_sequence/1

BBa_I756018
type: ComponentDefinition
persistent identity: BBa I756018
display id: BBa_I756018
version: 1
was generated by: iGEM to SBOL conversion
description: Lambda Operator in SV-40 intron
creator: Jack
created: 2007-08-15T11:00:00Z
modified: 2015-08-31T04:08:06Z
discontinued: false
dominant: false
group u list: _108_
m user id: 0
owner id: 2067
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Lambda Operator in SV-40 intron
mutable notes: Lambda Operator in SV-40 intron
mutable provenance: Lambda Operator in SV-40 intron
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I756018_sequence/1

BBa_K110004
type: ComponentDefinition
persistent identity: BBa K110004
display id: BBa_K110004
version: 1
was generated by: iGEM to SBOL conversion
description: Alpha-Cell Promoter Ste3
created: 2008-05-29T11:00:00Z
modified: 2015-05-08T01:09:08Z
mutable provenance: SGD
owned by: james
owned by: myers
mutable description: This promoter was isolated by taking 500bp upstream of the STE3 gene of S. cerevisiae. This promoter will be activated when alpha factor is expressed in an alpha haploid yeast cell.
discontinued: false
dominant: false
experience: experience/None
group u list: _201_
m user id: 0
owner id: 2669
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: This sequence was not edited
star: false
creator: James DiCarlo
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K110004_sequence/1

BBa_K165012
type: ComponentDefinition
persistent identity: BBa K165012
display id: BBa_K165012
version: 1
was generated by: iGEM to SBOL conversion
description: Gli1 binding sites
created: 2008-10-25T11:00:00Z
modified: 2015-05-08T01:10:55Z
mutable provenance: -
owned by: james
owned by: myers
mutable description: -
discontinued: false
dominant: false
group u list: _267_
m user id: 0
owner id: 2512
owning group id: 58
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: -
star: false
creator: John Szymanski
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K165012_sequence/1

BBa_J05218
type: ComponentDefinition
persistent identity: BBa J05218
display id: BBa_J05218
version: 1
was generated by: iGEM to SBOL conversion
description: Regulator for R4-cMaf
creator: Alexander Roth
created: 2005-09-07T11:00:00Z
modified: 2015-08-31T04:08:15Z
discontinued: false
dominant: false
group u list: _34_
m user id: 0
owner id: 412
owning group id: 34
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: binding sites for R1-CREBH and R2-YAP7
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J05218_sequence/1

BBa_K165011
type: ComponentDefinition
persistent identity: BBa K165011
display id: BBa_K165011
version: 1
was generated by: iGEM to SBOL conversion
description: Zif268-HIV binding sites (3)
created: 2008-10-25T11:00:00Z
modified: 2015-05-08T01:10:55Z
mutable provenance: -
owned by: james
owned by: myers
mutable description: -
discontinued: false
dominant: false
group u list: _267_
m user id: 0
owner id: 2512
owning group id: 58
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: -
star: false
creator: John Szymanski
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K165011_sequence/1

BBa_K110009
type: ComponentDefinition
persistent identity: BBa K110009
display id: BBa_K110009
version: 1
was generated by: iGEM to SBOL conversion
description: A-Cell Promoter STE2
created: 2008-05-29T11:00:00Z
modified: 2015-05-08T01:09:09Z
mutable provenance: SGD
owned by: james
owned by: myers
mutable description: This promoter was isolated by taking 500bp upstream of the MFA1 gene of S. cerevisiae. This promoter will be activated when A factor is expressed in an A haploid yeast cell.
discontinued: false
dominant: false
experience: experience/None
group u list: _201_
m user id: 0
owner id: 2669
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: This promotor is on the crick strand of the entered sequence.
star: false
creator: Rick Carrick, Ambhi Ganesan, Alex McMillan
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: MCM1
sequence annotation: STE12
sequence annotation: Mat Alpha2
sequence annotation: Ste2 Promoter
sequence: https://synbiohub.org/public/igem/BBa_K110009_sequence/1

MCM1
type: SequenceAnnotation
persistent identity: annotation1981500
display id: annotation1981500
version: 1
top level: BBa_K110009
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K110009/annotation1981500/range1981500/1

https://synbiohub.org/public/igem/BBa_K110009/annotation1981500/range1981500/1
type: Range
persistent identity: range1981500
display id: range1981500
version: 1
top level: BBa_K110009
direction: direction/forward
start: 283
end: 292
orientation: inline

STE12
type: SequenceAnnotation
persistent identity: annotation1981502
display id: annotation1981502
version: 1
top level: BBa_K110009
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K110009/annotation1981502/range1981502/1

https://synbiohub.org/public/igem/BBa_K110009/annotation1981502/range1981502/1
type: Range
persistent identity: range1981502
display id: range1981502
version: 1
top level: BBa_K110009
direction: direction/forward
start: 304
end: 312
orientation: inline

Mat Alpha2
type: SequenceAnnotation
persistent identity: annotation1981501
display id: annotation1981501
version: 1
top level: BBa_K110009
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K110009/annotation1981501/range1981501/1

https://synbiohub.org/public/igem/BBa_K110009/annotation1981501/range1981501/1
type: Range
persistent identity: range1981501
display id: range1981501
version: 1
top level: BBa_K110009
direction: direction/forward
start: 295
end: 301
orientation: inline

Mat Alpha2
type: SequenceAnnotation
persistent identity: annotation1981499
display id: annotation1981499
version: 1
top level: BBa_K110009
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K110009/annotation1981499/range1981499/1

https://synbiohub.org/public/igem/BBa_K110009/annotation1981499/range1981499/1
type: Range
persistent identity: range1981499
display id: range1981499
version: 1
top level: BBa_K110009
direction: direction/forward
start: 273
end: 280
orientation: inline

Ste2 Promoter
type: SequenceAnnotation
persistent identity: annotation1981498
display id: annotation1981498
version: 1
top level: BBa_K110009
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K110009/annotation1981498/range1981498/1

https://synbiohub.org/public/igem/BBa_K110009/annotation1981498/range1981498/1
type: Range
persistent identity: range1981498
display id: range1981498
version: 1
top level: BBa_K110009
direction: direction/forward
start: 1
end: 501
orientation: inline

BBa_K105029
type: ComponentDefinition
persistent identity: BBa K105029
display id: BBa_K105029
version: 1
was generated by: iGEM to SBOL conversion
description: cyc43 minimal promoter
created: 2008-10-25T11:00:00Z
modified: 2015-05-08T01:08:52Z
mutable provenance: Amplification and Mutation from the plasmid pAH3
owned by: james
owned by: myers
mutable description: This is a derivate of BBa_K105027. Due to a point mutation in the TATA box region the efficiency of this minimal promoter is only 43 % of the natural yeast CYC1 promoter.
discontinued: false
dominant: false
experience: experience/None
group u list: _253_
m user id: 0
owner id: 3313
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: This BioBrick contains the TATA-box with one point mutation and most of the 5'UTR (-139 to -36, relative to the translational start) of the natural CYC1 promoter. A Kozak sequence is not included and has thus to be added! Even if there isn't any need for this BioBrick to be fusible to other protein domains, we stayed with our design.
star: true
creator: Michael Wild
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: TATA-box
sequence annotation: A -> G
sequence: https://synbiohub.org/public/igem/BBa_K105029_sequence/1

TATA-box
type: SequenceAnnotation
persistent identity: annotation1989837
display id: annotation1989837
version: 1
top level: BBa_K105029
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K105029/annotation1989837/range1989837/1

https://synbiohub.org/public/igem/BBa_K105029/annotation1989837/range1989837/1
type: Range
persistent identity: range1989837
display id: range1989837
version: 1
top level: BBa_K105029
direction: direction/forward
start: 17
end: 23
orientation: inline

A -> G
type: SequenceAnnotation
persistent identity: annotation1989838
display id: annotation1989838
version: 1
top level: BBa_K105029
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_K105029/annotation1989838/range1989838/1

https://synbiohub.org/public/igem/BBa_K105029/annotation1989838/range1989838/1
type: Range
persistent identity: range1989838
display id: range1989838
version: 1
top level: BBa_K105029
direction: direction/forward
start: 23
end: 23
orientation: inline

BBa_I712004
type: ComponentDefinition
persistent identity: BBa I712004
display id: BBa_I712004
version: 1
was generated by: iGEM to SBOL conversion
description: CMV promoter
creator: Andrej Ondracka
created: 2007-10-18T11:00:00Z
modified: 2015-08-31T04:07:45Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _130_
m user id: 0
owner id: 1896
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: a constitutive expression promoter or use in mammalian cells. Ribosome binding site is included.
mutable notes: Cloned into pSB1AC3 vector.
mutable provenance: pcDNA3 by Invitrogen
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I712004_sequence/1

BBa_K165016
type: ComponentDefinition
persistent identity: BBa K165016
display id: BBa_K165016
version: 1
was generated by: iGEM to SBOL conversion
description: mCYC1 minimal yeast promoter
created: 2008-10-25T11:00:00Z
modified: 2015-05-08T01:10:55Z
mutable provenance: From David Drubin in the Silver Lab at Harvard
owned by: james
owned by: myers
mutable description: This minimal promoter can be used as a base for designing promoters by adding binding sites upstream.
discontinued: false
dominant: false
group u list: _267_
m user id: 0
owner id: 2512
owning group id: 58
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: -
star: false
creator: John Szymanski
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K165016_sequence/1

BBa_I764001
type: ComponentDefinition
persistent identity: BBa I764001
display id: BBa_I764001
version: 1
was generated by: iGEM to SBOL conversion
description: Ethanol regulated promoter AOX1
created: 2007-08-28T11:00:00Z
modified: 2015-08-31T04:08:09Z
mutable provenance: Pichia pastoris genome.
owned by: james
owned by: myers
mutable description: Needs to be added.
discontinued: false
dominant: false
group u list: _137_
m user id: 0
owner id: 1818
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: No special considerations.
star: false
creator: Dave Westenberg
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: primer_binding
sequence annotation: BioBrick
sequence: https://synbiohub.org/public/igem/BBa_I764001_sequence/1

primer_binding
type: SequenceAnnotation
persistent identity: annotation1943749
display id: annotation1943749
version: 1
top level: BBa_I764001
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_I764001/annotation1943749/range1943749/1

https://synbiohub.org/public/igem/BBa_I764001/annotation1943749/range1943749/1
type: Range
persistent identity: range1943749
display id: range1943749
version: 1
top level: BBa_I764001
direction: direction/forward
start: 1
end: 19
orientation: inline

BioBrick
type: SequenceAnnotation
persistent identity: annotation1943748
display id: annotation1943748
version: 1
top level: BBa_I764001
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I764001/annotation1943748/range1943748/1

https://synbiohub.org/public/igem/BBa_I764001/annotation1943748/range1943748/1
type: Range
persistent identity: range1943748
display id: range1943748
version: 1
top level: BBa_I764001
direction: direction/forward
start: 1
end: 867
orientation: inline

primer_binding
type: SequenceAnnotation
persistent identity: annotation1943750
display id: annotation1943750
version: 1
top level: BBa_I764001
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_I764001/annotation1943750/range1943750/1

https://synbiohub.org/public/igem/BBa_I764001/annotation1943750/range1943750/1
type: Range
persistent identity: range1943750
display id: range1943750
version: 1
top level: BBa_I764001
direction: direction/reverse
start: 844
end: 867
orientation: inline

BBa_I756021
type: ComponentDefinition
persistent identity: BBa I756021
display id: BBa_I756021
version: 1
was generated by: iGEM to SBOL conversion
description: CMV promoter with Lambda Operator
creator: Jack
created: 2007-08-15T11:00:00Z
modified: 2015-08-31T04:08:06Z
discontinued: false
dominant: false
group u list: _108_
m user id: 0
owner id: 2067
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: CMV promoter with Lambda Operator
mutable notes: CMV promoter with Lambda Operator
mutable provenance: CMV promoter with Lambda Operator
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I756021_sequence/1

BBa_I756016
type: ComponentDefinition
persistent identity: BBa I756016
display id: BBa_I756016
version: 1
was generated by: iGEM to SBOL conversion
description: CMV-tet promoter
creator: Jack
created: 2007-08-15T11:00:00Z
modified: 2015-08-31T04:08:06Z
discontinued: false
dominant: false
group u list: _108_
m user id: 0
owner id: 2067
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: CMV promoter with tet operator
mutable notes: CMV promoter with tet operator
mutable provenance: CMV promoter with tet operator
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: tet operator
sequence: https://synbiohub.org/public/igem/BBa_I756016_sequence/1

tet operator
type: SequenceAnnotation
persistent identity: annotation1941581
display id: annotation1941581
version: 1
top level: BBa_I756016
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I756016/annotation1941581/range1941581/1

https://synbiohub.org/public/igem/BBa_I756016/annotation1941581/range1941581/1
type: Range
persistent identity: range1941581
display id: range1941581
version: 1
direction: direction/forward
top level: BBa_I756016
start: 525
end: 578
orientation: inline

BBa_K165031
type: ComponentDefinition
persistent identity: BBa K165031
display id: BBa_K165031
version: 1
was generated by: iGEM to SBOL conversion
description: mCYC promoter plus LexA binding sites
created: 2008-10-28T12:00:00Z
modified: 2015-05-08T01:10:56Z
mutable provenance: Composite part was taken via PCR from a synthetic plasmid containing the sequence. The plasmid came from Lawrence Berkeley National Laboratory.
owned by: james
owned by: myers
mutable description: This is a composite part containing the minimal promoter mCYC preceded by multiple consecutive binding sites for the LexA binding domain.
discontinued: false
dominant: false
group u list: _267_
m user id: 0
owner id: 2510
owning group id: 58
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: It was necessary to design primers for use in PCR to remove this part from its host plasmid and transfer it to a standard Biobrick vector
star: false
creator: Aaron Glieberman
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K165031_sequence/1

BBa_M31201
type: ComponentDefinition
persistent identity: BBa M31201
display id: BBa_M31201
version: 1
was generated by: iGEM to SBOL conversion
description: Yeast CLB1 promoter region, G2/M cell cycle specific
created: 2008-05-13T11:00:00Z
modified: 2015-05-08T01:13:59Z
mutable provenance: Yeast consensus sequence from SGD chrVII:703140..703639
owned by: james
owned by: myers
mutable description: Description of CLB1 (from SGD): B-type cyclin involved in cell cycle progression; activates Cdc28p to promote the transition from G2 to M phase; accumulates during G2 and M, then targeted via a destruction box motif for ubiquitin-mediated degradation by the proteasome. More information found here: http://db.yeastgenome.org/cgi-bin/locus.pl?locus=CLB1
discontinued: false
dominant: false
group u list: _89_
m user id: 0
owner id: 624
owning group id: 89
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Part contains 500 bases upstream from the CLB1 ORF. Is NOT a minimal promoter, and has not been tested for composability.
star: false
creator: Katherine Aull
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_M31201_sequence/1

BBa_J24813
type: ComponentDefinition
persistent identity: BBa J24813
display id: BBa_J24813
version: 1
was generated by: iGEM to SBOL conversion
description: URA3 Promoter from S. cerevisiae
created: 2008-05-01T11:00:00Z
modified: 2015-08-31T04:08:43Z
mutable provenance: NCBI Sequence database: http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=Nucleotide&dopt=GenBank&val=172061
owned by: james
owned by: myers
mutable description: This is the whole regulatory region from the URA3 gene coding for OMP decarboxylase in S. cerevisiae budding yeast.
discontinued: false
dominant: false
group u list: _58_
m user id: 0
owner id: 2512
owning group id: 58
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: None
star: false
creator: John Szymanski
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J24813_sequence/1

BBa_K165000
type: ComponentDefinition
persistent identity: BBa K165000
display id: BBa_K165000
version: 1
was generated by: iGEM to SBOL conversion
description: MET 25 Promoter
created: 2008-10-24T11:00:00Z
modified: 2015-05-08T01:10:55Z
mutable provenance: From the Saccharomyces cerevisiae genome, by way of Caroline Ajo-Franklin.
owned by: james
owned by: myers
mutable description: The is a repressable promoter for use in Saccharomyces cerevisiae. It is repressed by the presence of methionine. When unrepresssed, is has a moderate level of activity.
discontinued: false
dominant: false
experience: experience/Works
group u list: _267_
m user id: 0
owner id: 2512
owning group id: 58
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: None.
star: false
creator: John Szymanski
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K165000_sequence/1

P0310
type: ComponentDefinition
persistent identity: BBa J63005
display id: BBa_J63005
version: 1
was generated by: iGEM to SBOL conversion
description: yeast ADH1 promoter
created: 2006-10-10T11:00:00Z
modified: 2015-08-31T01:56:26Z
mutable provenance: genomic DNA of ADH1 from S. cerevisiae
owned by: myers
owned by: james
mutable description: ADH1 promoter from S. cerevisiae
discontinued: false
dominant: true
experience: experience/Works
group u list: _97_
m user id: 0
owner id: 545
owning group id: 97
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: no changes to genomic sequence
star: true
creator: Caroline Ajo-Franklin
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: TATA Box
sequence annotation: Constitutive promoter
sequence: https://synbiohub.org/public/igem/BBa_J63005_sequence/1

TATA Box
type: SequenceAnnotation
persistent identity: annotation1902745
display id: annotation1902745
version: 1
top level: P0310
role: feature/dna
role: SO:0000110
location: https://synbiohub.org/public/igem/BBa_J63005/annotation1902745/range1902745/1

https://synbiohub.org/public/igem/BBa_J63005/annotation1902745/range1902745/1
type: Range
persistent identity: range1902745
display id: range1902745
version: 1
top level: P0310
direction: direction/forward
start: 1348
end: 1354
orientation: inline

Constitutive promoter
type: SequenceAnnotation
persistent identity: annotation1902744
display id: annotation1902744
version: 1
top level: P0310
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J63005/annotation1902744/range1902744/1

https://synbiohub.org/public/igem/BBa_J63005/annotation1902744/range1902744/1
type: Range
persistent identity: range1902744
display id: range1902744
version: 1
top level: P0310
direction: direction/forward
start: 1
end: 1445
orientation: inline

BBa_J05215
type: ComponentDefinition
persistent identity: BBa J05215
display id: BBa_J05215
version: 1
was generated by: iGEM to SBOL conversion
description: Regulator for R1-CREBH
creator: Alexander Roth
created: 2005-09-07T11:00:00Z
modified: 2015-08-31T04:08:15Z
discontinued: false
dominant: false
group u list: _34_
m user id: 0
owner id: 412
owning group id: 34
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: binding sites for R3-ATF6 and R2-YAP7
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J05215_sequence/1

BBa_K122000
type: ComponentDefinition
persistent identity: BBa K122000
display id: BBa_K122000
version: 1
was generated by: iGEM to SBOL conversion
description: pPGK1
created: 2008-10-20T11:00:00Z
modified: 2015-05-08T01:09:42Z
mutable provenance: This part was obtained from yeast genomic DNA.
owned by: james
owned by: myers
mutable description: This is the 1500 bp upstream of the yeast PGK1 coding region. This is a putative constitutive promoter.
discontinued: false
dominant: false
experience: experience/None
group u list: _218_
m user id: 0
owner id: 3326
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: Source: Saccharomyces cerevisiae genomic DNA.
star: false
creator: David Ouyang
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K122000_sequence/1

Oct-4 prom
type: ComponentDefinition
persistent identity: BBa I10498
display id: BBa_I10498
version: 1
was generated by: iGEM to SBOL conversion
description: Oct-4 promoter
creator: Amanda Morris
created: 2006-05-13T11:00:00Z
modified: 2015-08-31T04:07:30Z
discontinued: false
dominant: false
experience: experience/None
group u list: _89_
m user id: 0
owner id: 622
owning group id: 89
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: The part codes for the human Oct-4 promoter, which regulates expression of the Oct-4 gene. The Oct-4 gene is essential for pluripotency in cells and is downregulated once differentiation occurs. Sequence information is from GenBank (NM_002701) and shows the cis-regulatory mRNA that controls transcriptional activation of the human Oct-4 promoter.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I10498_sequence/1

pTEF
type: ComponentDefinition
persistent identity: BBa K165037
display id: BBa_K165037
version: 1
was generated by: iGEM to SBOL conversion
description: TEF2 yeast constitutive promoter
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:10:56Z
mutable provenance: From the yeast genome using promoter definition from: Mumberg, Muller, and Funk: http://dx.doi.org/10.1016/0378-1119(95)00037-7
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: This is a high level constitutive promoter from the yeast gene TEF2.
discontinued: false
dominant: false
experience: experience/Works
group u list: _267_
m user id: 0
owner id: 2512
owning group id: 58
sample status: In stock
status: status/Available
bookmark: true
mutable notes: -
star: true
creator: John Szymanski
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K165037_sequence/1

BBa_K105028
type: ComponentDefinition
persistent identity: BBa K105028
display id: BBa_K105028
version: 1
was generated by: iGEM to SBOL conversion
description: cyc70 minimal promoter
created: 2008-10-25T11:00:00Z
modified: 2015-05-08T01:08:52Z
mutable provenance: Amplification and Mutation from the plasmid pAH3
owned by: james
owned by: myers
mutable description: This is a derivate of BBa_K105027. Due to a point mutation in the TATA box region the efficiency of this minimal promoter is only 70 % of the natural yeast CYC1 promoter.
discontinued: false
dominant: false
experience: experience/None
group u list: _253_
m user id: 0
owner id: 3313
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: This BioBrick contains the TATA-box with one point mutation and most of the 5'UTR (-139 to -36, relative to the translational start) of the natural CYC1 promoter. A Kozak sequence is not included and has thus to be added! Even if there isn't any need for this BioBrick to be fusible to other protein domains, we stayed with our design.
star: true
creator: Michael Wild
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: TATA-box
sequence annotation: A->C
sequence: https://synbiohub.org/public/igem/BBa_K105028_sequence/1

TATA-box
type: SequenceAnnotation
persistent identity: annotation1989835
display id: annotation1989835
version: 1
top level: BBa_K105028
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K105028/annotation1989835/range1989835/1

https://synbiohub.org/public/igem/BBa_K105028/annotation1989835/range1989835/1
type: Range
persistent identity: range1989835
display id: range1989835
version: 1
top level: BBa_K105028
direction: direction/forward
start: 17
end: 23
orientation: inline

A->C
type: SequenceAnnotation
persistent identity: annotation1989836
display id: annotation1989836
version: 1
top level: BBa_K105028
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_K105028/annotation1989836/range1989836/1

https://synbiohub.org/public/igem/BBa_K105028/annotation1989836/range1989836/1
type: Range
persistent identity: range1989836
display id: range1989836
version: 1
top level: BBa_K105028
direction: direction/forward
start: 24
end: 24
orientation: inline

BBa_K105030
type: ComponentDefinition
persistent identity: BBa K105030
display id: BBa_K105030
version: 1
was generated by: iGEM to SBOL conversion
description: cyc28 minimal promoter
created: 2008-10-25T11:00:00Z
modified: 2015-05-08T01:08:52Z
mutable provenance: Amplification and Mutation from the plasmid pAH3
owned by: james
owned by: myers
mutable description: This is a derivate of BBa_K105027. Due to a point mutation in the TATA box region the efficiency of this minimal promoter is only 28 % of the natural yeast CYC1 promoter.
discontinued: false
dominant: false
experience: experience/None
group u list: _253_
m user id: 0
owner id: 3313
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: This BioBrick contains the TATA-box with one point mutation and most of the 5'UTR (-139 to -36, relative to the translational start) of the natural CYC1 promoter. A Kozak sequence is not included and has thus to be added! Even if there isn't any need for this BioBrick to be fusible to other protein domains, we stayed with our design.
star: true
creator: Michael Wild
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: A -> T
sequence annotation: TATA-box
sequence: https://synbiohub.org/public/igem/BBa_K105030_sequence/1

A -> T
type: SequenceAnnotation
persistent identity: annotation1989840
display id: annotation1989840
version: 1
top level: BBa_K105030
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_K105030/annotation1989840/range1989840/1

https://synbiohub.org/public/igem/BBa_K105030/annotation1989840/range1989840/1
type: Range
persistent identity: range1989840
display id: range1989840
version: 1
top level: BBa_K105030
direction: direction/forward
start: 22
end: 22
orientation: inline

TATA-box
type: SequenceAnnotation
persistent identity: annotation1989839
display id: annotation1989839
version: 1
top level: BBa_K105030
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K105030/annotation1989839/range1989839/1

https://synbiohub.org/public/igem/BBa_K105030/annotation1989839/range1989839/1
type: Range
persistent identity: range1989839
display id: range1989839
version: 1
top level: BBa_K105030
direction: direction/forward
start: 17
end: 23
orientation: inline

BBa_K165041
type: ComponentDefinition
persistent identity: BBa K165041
display id: BBa_K165041
version: 1
was generated by: iGEM to SBOL conversion
description: Zif268-HIV binding sites + TEF constitutive yeast promoter
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:10:56Z
mutable provenance: -
owned by: james
owned by: myers
mutable description: -
discontinued: false
dominant: false
experience: experience/None
group u list: _267_
m user id: 0
owner id: 2512
owning group id: 58
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: -
star: true
creator: John Szymanski
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
component: BBa_K165037
component: BBa_K165011
sequence annotation: BBa_K165037
sequence annotation: BBa_K165011
sequence: https://synbiohub.org/public/igem/BBa_K165041_sequence/1

BBa_K165037
type: Component
persistent identity: component1996558
display id: component1996558
version: 1
top level: BBa_K165041
access: public
definition: pTEF

BBa_K165011
type: Component
persistent identity: component1996557
display id: component1996557
version: 1
top level: BBa_K165041
access: public
definition: BBa_K165011

BBa_K165037
type: SequenceAnnotation
persistent identity: annotation1996558
display id: annotation1996558
version: 1
top level: BBa_K165041
location: https://synbiohub.org/public/igem/BBa_K165041/annotation1996558/range1996558/1
component: BBa_K165037

https://synbiohub.org/public/igem/BBa_K165041/annotation1996558/range1996558/1
type: Range
persistent identity: range1996558
display id: range1996558
version: 1
top level: BBa_K165041
direction: direction/reverse
start: 55
end: 457
orientation: inline

BBa_K165011
type: SequenceAnnotation
persistent identity: annotation1996557
display id: annotation1996557
version: 1
top level: BBa_K165041
location: https://synbiohub.org/public/igem/BBa_K165041/annotation1996557/range1996557/1
component: BBa_K165011

https://synbiohub.org/public/igem/BBa_K165041/annotation1996557/range1996557/1
type: Range
persistent identity: range1996557
display id: range1996557
version: 1
top level: BBa_K165041
direction: direction/reverse
start: 1
end: 46
orientation: inline

pTEF
type: ComponentDefinition
persistent identity: BBa K124001
display id: BBa_K124001
version: 1
was generated by: iGEM to SBOL conversion
description: Yeast TEF2 Constitutive Promoter
created: 2008-06-30T11:00:00Z
modified: 2015-05-08T01:09:44Z
mutable provenance: Mumberg, Muller, and Funk. p413 pTEF http://dx.doi.org/10.1016/0378-1119(95)00037-7
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: This is a mid-high level constitutive promoter from the yeast gene TEF2.
discontinued: true
dominant: false
experience: experience/None
group u list: _267_
m user id: 0
owner id: 2512
owning group id: 58
sample status: Discontinued
status: status/Deleted
bookmark: false
mutable notes: none
star: false
creator: John Szymanski
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K124001_sequence/1

BBa_I756019
type: ComponentDefinition
persistent identity: BBa I756019
display id: BBa_I756019
version: 1
was generated by: iGEM to SBOL conversion
description: Lac Operator in SV-40 intron
creator: Jack
created: 2007-08-15T11:00:00Z
modified: 2015-08-31T04:08:06Z
discontinued: false
dominant: true
group u list: _108_
m user id: 0
owner id: 2067
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Lac Operator in SV-40 intron
mutable notes: Lac Operator in SV-40 intron
mutable provenance: Lac Operator in SV-40 intron
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I756019_sequence/1

BBa_K165039
type: ComponentDefinition
persistent identity: BBa K165039
display id: BBa_K165039
version: 1
was generated by: iGEM to SBOL conversion
description: Zif268-HIV binding sites + ADH1 yeast promoter
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:10:56Z
mutable provenance: Constructed in our lab with parts from Caroline Ajo-Franklin and David Drubin.
owned by: james
owned by: myers
mutable description: The Zif268-HIV transcription factor binding sites on the ADH1 constitutive promoter. Transcription regulation should be constitutive plus regulated by Zif268-HIV-binding transcription factors.
discontinued: false
dominant: false
group u list: _267_
m user id: 0
owner id: 2512
owning group id: 58
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: -
star: false
creator: John Szymanski
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
component: BBa_K165015
component: BBa_K165011
sequence annotation: BBa_K165015
sequence annotation: BBa_K165011
sequence: https://synbiohub.org/public/igem/BBa_K165039_sequence/1

BBa_K165015
type: Component
persistent identity: component1995517
display id: component1995517
version: 1
top level: BBa_K165039
access: public
definition: BBa_K165015

BBa_K165011
type: Component
persistent identity: component1995516
display id: component1995516
version: 1
top level: BBa_K165039
access: public
definition: BBa_K165011

BBa_K165015
type: SequenceAnnotation
persistent identity: annotation1995517
display id: annotation1995517
version: 1
top level: BBa_K165039
location: https://synbiohub.org/public/igem/BBa_K165039/annotation1995517/range1995517/1
component: BBa_K165015

https://synbiohub.org/public/igem/BBa_K165039/annotation1995517/range1995517/1
type: Range
persistent identity: range1995517
display id: range1995517
version: 1
top level: BBa_K165039
direction: direction/reverse
start: 55
end: 1499
orientation: inline

BBa_K165011
type: SequenceAnnotation
persistent identity: annotation1995516
display id: annotation1995516
version: 1
top level: BBa_K165039
location: https://synbiohub.org/public/igem/BBa_K165039/annotation1995516/range1995516/1
component: BBa_K165011

https://synbiohub.org/public/igem/BBa_K165039/annotation1995516/range1995516/1
type: Range
persistent identity: range1995516
display id: range1995516
version: 1
top level: BBa_K165039
direction: direction/reverse
start: 1
end: 46
orientation: inline

BBa_K110006
type: ComponentDefinition
persistent identity: BBa K110006
display id: BBa_K110006
version: 1
was generated by: iGEM to SBOL conversion
description: Alpha-Cell Promoter MF(ALPHA)1
created: 2008-05-29T11:00:00Z
modified: 2015-05-08T01:09:08Z
mutable provenance: SGD
owned by: james
owned by: myers
mutable description: This promoter was isolated by taking 500bp upstream of the MF(ALPHA)1 gene of S. cerevisiae. This promoter will be activated when alpha factor is expressed in an alpha haploid yeast cell.
discontinued: false
dominant: false
experience: experience/None
group u list: _201_
m user id: 0
owner id: 2669
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: This gene is on the Watson strand of the entered sequence.
star: true
creator: James DiCarlo
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: MCM1
sequence annotation: MF(alpha)1
sequence: https://synbiohub.org/public/igem/BBa_K110006_sequence/1

MCM1
type: SequenceAnnotation
persistent identity: annotation1997688
display id: annotation1997688
version: 1
top level: BBa_K110006
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K110006/annotation1997688/range1997688/1

https://synbiohub.org/public/igem/BBa_K110006/annotation1997688/range1997688/1
type: Range
persistent identity: range1997688
display id: range1997688
version: 1
top level: BBa_K110006
direction: direction/forward
start: 19
end: 34
orientation: inline

MCM1
type: SequenceAnnotation
persistent identity: annotation1997690
display id: annotation1997690
version: 1
top level: BBa_K110006
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K110006/annotation1997690/range1997690/1

https://synbiohub.org/public/igem/BBa_K110006/annotation1997690/range1997690/1
type: Range
persistent identity: range1997690
display id: range1997690
version: 1
top level: BBa_K110006
direction: direction/forward
start: 92
end: 102
orientation: inline

MF(alpha)1
type: SequenceAnnotation
persistent identity: annotation1997687
display id: annotation1997687
version: 1
top level: BBa_K110006
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K110006/annotation1997687/range1997687/1

https://synbiohub.org/public/igem/BBa_K110006/annotation1997687/range1997687/1
type: Range
persistent identity: range1997687
display id: range1997687
version: 1
top level: BBa_K110006
direction: direction/forward
start: 1
end: 501
orientation: inline

MCM1
type: SequenceAnnotation
persistent identity: annotation1997689
display id: annotation1997689
version: 1
top level: BBa_K110006
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K110006/annotation1997689/range1997689/1

https://synbiohub.org/public/igem/BBa_K110006/annotation1997689/range1997689/1
type: Range
persistent identity: range1997689
display id: range1997689
version: 1
top level: BBa_K110006
direction: direction/forward
start: 136
end: 161
orientation: inline

BBa_K165033
type: ComponentDefinition
persistent identity: BBa K165033
display id: BBa_K165033
version: 1
was generated by: iGEM to SBOL conversion
description: YY1 binding sites + mCYC promoter
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:10:56Z
mutable provenance: Made in our lab with parts from Caroline Ajo-Franklin and David Drubin.
owned by: james
owned by: myers
mutable description: The YY1 transcription factor binding sites on the mCYC minimal promoter. All transcription regulation comes from YY1-binding transcription factors.
discontinued: false
dominant: false
group u list: _267_
m user id: 0
owner id: 2512
owning group id: 58
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: -
star: false
creator: John Szymanski
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
component: BBa_K165016
component: BBa_K165013
sequence annotation: BBa_K165013
sequence annotation: BBa_K165016
sequence: https://synbiohub.org/public/igem/BBa_K165033_sequence/1

BBa_K165016
type: Component
persistent identity: component1995403
display id: component1995403
version: 1
top level: BBa_K165033
access: public
definition: BBa_K165016

BBa_K165013
type: Component
persistent identity: component1995402
display id: component1995402
version: 1
top level: BBa_K165033
access: public
definition: BBa_K165013

BBa_K165013
type: SequenceAnnotation
persistent identity: annotation1995402
display id: annotation1995402
version: 1
top level: BBa_K165033
location: https://synbiohub.org/public/igem/BBa_K165033/annotation1995402/range1995402/1
component: BBa_K165013

https://synbiohub.org/public/igem/BBa_K165033/annotation1995402/range1995402/1
type: Range
persistent identity: range1995402
display id: range1995402
version: 1
top level: BBa_K165033
direction: direction/reverse
start: 1
end: 51
orientation: inline

BBa_K165016
type: SequenceAnnotation
persistent identity: annotation1995403
display id: annotation1995403
version: 1
top level: BBa_K165033
location: https://synbiohub.org/public/igem/BBa_K165033/annotation1995403/range1995403/1
component: BBa_K165016

https://synbiohub.org/public/igem/BBa_K165033/annotation1995403/range1995403/1
type: Range
persistent identity: range1995403
display id: range1995403
version: 1
top level: BBa_K165033
direction: direction/reverse
start: 60
end: 304
orientation: inline

BBa_K165040
type: ComponentDefinition
persistent identity: BBa K165040
display id: BBa_K165040
version: 1
was generated by: iGEM to SBOL conversion
description: Gli1 binding sites + TEF constitutive yeast promoter
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:10:56Z
mutable provenance: -
owned by: james
owned by: myers
mutable description: The Gli1 transcription factor binding sites on the TEF2 constitutive promoter. Transcription regulation should be constitutive plus regulated by Gli1-binding transcription factors.
discontinued: false
dominant: false
group u list: _267_
m user id: 0
owner id: 2512
owning group id: 58
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: -
star: false
creator: John Szymanski
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
component: BBa_K165012
component: BBa_K165037
sequence annotation: BBa_K165037
sequence annotation: BBa_K165012
sequence: https://synbiohub.org/public/igem/BBa_K165040_sequence/1

BBa_K165012
type: Component
persistent identity: component1996555
display id: component1996555
version: 1
top level: BBa_K165040
access: public
definition: BBa_K165012

BBa_K165037
type: Component
persistent identity: component1996556
display id: component1996556
version: 1
top level: BBa_K165040
access: public
definition: pTEF

BBa_K165037
type: SequenceAnnotation
persistent identity: annotation1996556
display id: annotation1996556
version: 1
top level: BBa_K165040
location: https://synbiohub.org/public/igem/BBa_K165040/annotation1996556/range1996556/1
component: BBa_K165037

https://synbiohub.org/public/igem/BBa_K165040/annotation1996556/range1996556/1
type: Range
persistent identity: range1996556
display id: range1996556
version: 1
top level: BBa_K165040
direction: direction/reverse
start: 136
end: 538
orientation: inline

BBa_K165012
type: SequenceAnnotation
persistent identity: annotation1996555
display id: annotation1996555
version: 1
top level: BBa_K165040
location: https://synbiohub.org/public/igem/BBa_K165040/annotation1996555/range1996555/1
component: BBa_K165012

https://synbiohub.org/public/igem/BBa_K165040/annotation1996555/range1996555/1
type: Range
persistent identity: range1996555
display id: range1996555
version: 1
top level: BBa_K165040
direction: direction/reverse
start: 1
end: 127
orientation: inline

BBa_I766214
type: ComponentDefinition
persistent identity: BBa I766214
display id: BBa_I766214
version: 1
was generated by: iGEM to SBOL conversion
description: pGal1
created: 2007-10-21T11:00:00Z
modified: 2015-08-31T04:08:09Z
mutable provenance: yeast
owned by: james
owned by: myers
mutable description: pGAl1 promoter region
discontinued: false
dominant: false
group u list: _155_
m user id: 0
owner id: 1931
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: none
star: false
creator: Nili Sommovilla
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I766214_sequence/1

BBa_K110008
type: ComponentDefinition
persistent identity: BBa K110008
display id: BBa_K110008
version: 1
was generated by: iGEM to SBOL conversion
description: A-Cell Promoter MFA1
created: 2008-05-29T11:00:00Z
modified: 2015-05-08T01:09:09Z
mutable provenance: SGD
owned by: james
owned by: myers
mutable description: This promoter was isolated by taking 500bp upstream of the MFA1 gene of S. cerevisiae. This promoter will be activated when A factor is expressed in an A haploid yeast cell.
discontinued: false
dominant: false
experience: experience/None
group u list: _201_
m user id: 0
owner id: 2669
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: This gene is on the Watson strand of the entered sequence.
star: false
creator: James DiCarlo
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: MATalpha2
sequence annotation: STE12
sequence annotation: MCM1
sequence annotation: MFA1 Promoter
sequence: https://synbiohub.org/public/igem/BBa_K110008_sequence/1

MATalpha2
type: SequenceAnnotation
persistent identity: annotation1981089
display id: annotation1981089
version: 1
top level: BBa_K110008
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K110008/annotation1981089/range1981089/1

https://synbiohub.org/public/igem/BBa_K110008/annotation1981089/range1981089/1
type: Range
persistent identity: range1981089
display id: range1981089
version: 1
top level: BBa_K110008
direction: direction/forward
start: 289
end: 297
orientation: inline

STE12
type: SequenceAnnotation
persistent identity: annotation1981090
display id: annotation1981090
version: 1
top level: BBa_K110008
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K110008/annotation1981090/range1981090/1

https://synbiohub.org/public/igem/BBa_K110008/annotation1981090/range1981090/1
type: Range
persistent identity: range1981090
display id: range1981090
version: 1
top level: BBa_K110008
direction: direction/forward
start: 302
end: 309
orientation: inline

MCM1
type: SequenceAnnotation
persistent identity: annotation1981088
display id: annotation1981088
version: 1
top level: BBa_K110008
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K110008/annotation1981088/range1981088/1

https://synbiohub.org/public/igem/BBa_K110008/annotation1981088/range1981088/1
type: Range
persistent identity: range1981088
display id: range1981088
version: 1
top level: BBa_K110008
direction: direction/forward
start: 277
end: 287
orientation: inline

MATalpha2
type: SequenceAnnotation
persistent identity: annotation1981087
display id: annotation1981087
version: 1
top level: BBa_K110008
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K110008/annotation1981087/range1981087/1

https://synbiohub.org/public/igem/BBa_K110008/annotation1981087/range1981087/1
type: Range
persistent identity: range1981087
display id: range1981087
version: 1
top level: BBa_K110008
direction: direction/forward
start: 267
end: 276
orientation: inline

MFA1 Promoter
type: SequenceAnnotation
persistent identity: annotation1981086
display id: annotation1981086
version: 1
top level: BBa_K110008
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K110008/annotation1981086/range1981086/1

https://synbiohub.org/public/igem/BBa_K110008/annotation1981086/range1981086/1
type: Range
persistent identity: range1981086
display id: range1981086
version: 1
top level: BBa_K110008
direction: direction/forward
start: 1
end: 501
orientation: inline

BBa_I766557
type: ComponentDefinition
persistent identity: BBa I766557
display id: BBa_I766557
version: 1
was generated by: iGEM to SBOL conversion
description: pSte5 (Weak) Promoter
created: 2007-08-15T11:00:00Z
modified: 2015-08-31T04:08:10Z
mutable provenance: endogenous promoter for Sterile 5
owned by: james
owned by: myers
mutable description: low expression, constitutive promoter in yeast
discontinued: false
dominant: false
experience: experience/Works
group u list: _155_
m user id: 0
owner id: 1931
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: none
star: false
creator: Nili Sommovilla
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I766557_sequence/1

BBa_K165042
type: ComponentDefinition
persistent identity: BBa K165042
display id: BBa_K165042
version: 1
was generated by: iGEM to SBOL conversion
description: Gli1 binding sites + MET25 inducible yeast promoter
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:10:56Z
mutable provenance: -
owned by: james
owned by: myers
mutable description: -
discontinued: false
dominant: false
group u list: _267_
m user id: 0
owner id: 2512
owning group id: 58
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: -
star: false
creator: John Szymanski
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
component: BBa_K165000
component: BBa_K165012
sequence annotation: BBa_K165012
sequence annotation: BBa_K165000
sequence: https://synbiohub.org/public/igem/BBa_K165042_sequence/1

BBa_K165000
type: Component
persistent identity: component1996563
display id: component1996563
version: 1
top level: BBa_K165042
access: public
definition: BBa_K165000

BBa_K165012
type: Component
persistent identity: component1996562
display id: component1996562
version: 1
top level: BBa_K165042
access: public
definition: BBa_K165012

BBa_K165012
type: SequenceAnnotation
persistent identity: annotation1996562
display id: annotation1996562
version: 1
top level: BBa_K165042
location: https://synbiohub.org/public/igem/BBa_K165042/annotation1996562/range1996562/1
component: BBa_K165012

https://synbiohub.org/public/igem/BBa_K165042/annotation1996562/range1996562/1
type: Range
persistent identity: range1996562
display id: range1996562
version: 1
top level: BBa_K165042
direction: direction/reverse
start: 1
end: 127
orientation: inline

BBa_K165000
type: SequenceAnnotation
persistent identity: annotation1996563
display id: annotation1996563
version: 1
top level: BBa_K165042
location: https://synbiohub.org/public/igem/BBa_K165042/annotation1996563/range1996563/1
component: BBa_K165000

https://synbiohub.org/public/igem/BBa_K165042/annotation1996563/range1996563/1
type: Range
persistent identity: range1996563
display id: range1996563
version: 1
top level: BBa_K165042
direction: direction/reverse
start: 136
end: 522
orientation: inline

BBa_K076017
type: ComponentDefinition
persistent identity: BBa K076017
display id: BBa_K076017
version: 1
was generated by: iGEM to SBOL conversion
description: Ubc Promoter
creator: Available
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:08:32Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _215_
m user id: 0
owner id: 3466
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is a stable constitutive promoter.
mutable notes: Using this promoter allows for the constant expression of a reporter gene or a selectable antibiotic.
mutable provenance: This part was obtained from members of the Weiss Lab.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K076017_sequence/1

BBa_K124000
type: ComponentDefinition
persistent identity: BBa K124000
display id: BBa_K124000
version: 1
was generated by: iGEM to SBOL conversion
description: pCYC Yeast Promoter
created: 2008-06-30T11:00:00Z
modified: 2015-05-08T01:09:44Z
mutable provenance: From Mumberg, Muller, and Funk p413 pCYC. See ref. above
owned by: james
owned by: myers
mutable description: The promoter from the yeast gene CYC1. Activity characterized here: http://dx.doi.org/10.1016/0378-1119(95)00037-7.
discontinued: false
dominant: false
group u list: _221_
m user id: 0
owner id: 2512
owning group id: 58
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: none
star: false
creator: John Szymanski
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K124000_sequence/1

BBa_K110014
type: ComponentDefinition
persistent identity: BBa K110014
display id: BBa_K110014
version: 1
was generated by: iGEM to SBOL conversion
description: A-Cell Promoter MFA2 (backwards)
created: 2008-08-26T11:00:00Z
modified: 2015-05-08T01:09:09Z
mutable provenance: SGD
owned by: james
owned by: myers
mutable description: This is the same part as BBa_K110007 except it has Prefix and Suffix regions switched
discontinued: false
dominant: false
experience: experience/None
group u list: _201_
m user id: 0
owner id: 2669
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: This was taken from SGD
star: false
creator: James DiCarlo
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K110014_sequence/1

BBa_K165013
type: ComponentDefinition
persistent identity: BBa K165013
display id: BBa_K165013
version: 1
was generated by: iGEM to SBOL conversion
description: YY1 binding sites
created: 2008-10-25T11:00:00Z
modified: 2015-05-08T01:10:55Z
mutable provenance: -
owned by: james
owned by: myers
mutable description: -
discontinued: false
dominant: false
group u list: _267_
m user id: 0
owner id: 2512
owning group id: 58
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: -
star: false
creator: John Szymanski
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K165013_sequence/1

BBa_I756020
type: ComponentDefinition
persistent identity: BBa I756020
display id: BBa_I756020
version: 1
was generated by: iGEM to SBOL conversion
description: Tet Operator in SV-40 intron
creator: Jack
created: 2007-08-15T11:00:00Z
modified: 2015-08-31T04:08:06Z
discontinued: false
dominant: false
group u list: _108_
m user id: 0
owner id: 2067
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Tet Operator in SV-40 intron
mutable notes: Tet Operator in SV-40 intron
mutable provenance: Tet Operator in SV-40 intron
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I756020_sequence/1

BBa_K165036
type: ComponentDefinition
persistent identity: BBa K165036
display id: BBa_K165036
version: 1
was generated by: iGEM to SBOL conversion
description: Gli1 bs + LexA bs + mCYC promoter
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:10:56Z
mutable provenance: Constructed in our lab with parts from Caroline Ajo-Franklin and David Drubin.
owned by: james
owned by: myers
mutable description: The Gli1 and LexA transcription factor binding sites on the mCYC minimal promoter (in that order.) Transcription regulation comes from Gli1 and LexA-binding transcription factors.
discontinued: false
dominant: false
group u list: _267_
m user id: 0
owner id: 2512
owning group id: 58
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: -
star: false
creator: John Szymanski
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
component: BBa_K165012
component: BBa_K165031
sequence annotation: BBa_K165031
sequence annotation: BBa_K165012
sequence: https://synbiohub.org/public/igem/BBa_K165036_sequence/1

BBa_K165012
type: Component
persistent identity: component1995510
display id: component1995510
version: 1
top level: BBa_K165036
access: public
definition: BBa_K165012

BBa_K165031
type: Component
persistent identity: component1995511
display id: component1995511
version: 1
top level: BBa_K165036
access: public
definition: BBa_K165031

BBa_K165031
type: SequenceAnnotation
persistent identity: annotation1995511
display id: annotation1995511
version: 1
top level: BBa_K165036
location: https://synbiohub.org/public/igem/BBa_K165036/annotation1995511/range1995511/1
component: BBa_K165031

https://synbiohub.org/public/igem/BBa_K165036/annotation1995511/range1995511/1
type: Range
persistent identity: range1995511
display id: range1995511
version: 1
top level: BBa_K165036
direction: direction/reverse
start: 136
end: 538
orientation: inline

BBa_K165012
type: SequenceAnnotation
persistent identity: annotation1995510
display id: annotation1995510
version: 1
top level: BBa_K165036
location: https://synbiohub.org/public/igem/BBa_K165036/annotation1995510/range1995510/1
component: BBa_K165012

https://synbiohub.org/public/igem/BBa_K165036/annotation1995510/range1995510/1
type: Range
persistent identity: range1995510
display id: range1995510
version: 1
top level: BBa_K165036
direction: direction/reverse
start: 1
end: 127
orientation: inline

BBa_K110015
type: ComponentDefinition
persistent identity: BBa K110015
display id: BBa_K110015
version: 1
was generated by: iGEM to SBOL conversion
description: A-Cell Promoter MFA1 (RtL)
created: 2008-08-26T11:00:00Z
modified: 2015-05-08T01:09:09Z
mutable provenance: sgd
owned by: james
owned by: myers
mutable description: This is the same sequence as MFA1 promotor(BBa_K110008), but with suffix and prefix switched
discontinued: false
dominant: false
experience: experience/None
group u list: _201_
m user id: 0
owner id: 2669
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: This is directlyh from the DNA sequence
star: false
creator: Rick Carrick, Ambhi Ganesan, Alex McMillan
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: STE12
sequence annotation: MCM1
sequence annotation: MATalpha2
sequence annotation: MFA1 promoter (RtL)
sequence: https://synbiohub.org/public/igem/BBa_K110015_sequence/1

STE12
type: SequenceAnnotation
persistent identity: annotation1981506
display id: annotation1981506
version: 1
top level: BBa_K110015
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K110015/annotation1981506/range1981506/1

https://synbiohub.org/public/igem/BBa_K110015/annotation1981506/range1981506/1
type: Range
persistent identity: range1981506
display id: range1981506
version: 1
top level: BBa_K110015
direction: direction/forward
start: 302
end: 309
orientation: inline

MCM1
type: SequenceAnnotation
persistent identity: annotation1981505
display id: annotation1981505
version: 1
top level: BBa_K110015
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K110015/annotation1981505/range1981505/1

https://synbiohub.org/public/igem/BBa_K110015/annotation1981505/range1981505/1
type: Range
persistent identity: range1981505
display id: range1981505
version: 1
top level: BBa_K110015
direction: direction/forward
start: 277
end: 287
orientation: inline

MATalpha2
type: SequenceAnnotation
persistent identity: annotation1981507
display id: annotation1981507
version: 1
top level: BBa_K110015
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K110015/annotation1981507/range1981507/1

https://synbiohub.org/public/igem/BBa_K110015/annotation1981507/range1981507/1
type: Range
persistent identity: range1981507
display id: range1981507
version: 1
top level: BBa_K110015
direction: direction/forward
start: 289
end: 297
orientation: inline

MATalpha2
type: SequenceAnnotation
persistent identity: annotation1981504
display id: annotation1981504
version: 1
top level: BBa_K110015
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K110015/annotation1981504/range1981504/1

https://synbiohub.org/public/igem/BBa_K110015/annotation1981504/range1981504/1
type: Range
persistent identity: range1981504
display id: range1981504
version: 1
top level: BBa_K110015
direction: direction/forward
start: 267
end: 276
orientation: inline

MFA1 promoter (RtL)
type: SequenceAnnotation
persistent identity: annotation1981503
display id: annotation1981503
version: 1
top level: BBa_K110015
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K110015/annotation1981503/range1981503/1

https://synbiohub.org/public/igem/BBa_K110015/annotation1981503/range1981503/1
type: Range
persistent identity: range1981503
display id: range1981503
version: 1
top level: BBa_K110015
direction: direction/forward
start: 1
end: 401
orientation: inline

BBa_K105031
type: ComponentDefinition
persistent identity: BBa K105031
display id: BBa_K105031
version: 1
was generated by: iGEM to SBOL conversion
description: cyc16 minimal promoter
created: 2008-10-25T11:00:00Z
modified: 2015-05-08T01:08:52Z
mutable provenance: Amplification and Mutation from the plasmid pAH3
owned by: james
owned by: myers
mutable description: This is a derivate of BBa_K105027. Due to a point mutation in the TATA box region the efficiency of this minimal promoter is only 16 % of the natural yeast CYC1 promoter.
discontinued: false
dominant: false
experience: experience/None
group u list: _253_
m user id: 0
owner id: 3313
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: This BioBrick contains the TATA-box with one point mutation and most of the 5'UTR (-139 to -36, relative to the translational start) of the natural CYC1 promoter. A Kozak sequence is not included and has thus to be added! Even if there isn't any need for this BioBrick to be fusible to other protein domains, we stayed with our design.
star: true
creator: Michael Wild
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: A -> G
sequence annotation: TATA-box
sequence: https://synbiohub.org/public/igem/BBa_K105031_sequence/1

A -> G
type: SequenceAnnotation
persistent identity: annotation1989845
display id: annotation1989845
version: 1
top level: BBa_K105031
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_K105031/annotation1989845/range1989845/1

https://synbiohub.org/public/igem/BBa_K105031/annotation1989845/range1989845/1
type: Range
persistent identity: range1989845
display id: range1989845
version: 1
top level: BBa_K105031
direction: direction/forward
start: 22
end: 22
orientation: inline

TATA-box
type: SequenceAnnotation
persistent identity: annotation1989843
display id: annotation1989843
version: 1
top level: BBa_K105031
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K105031/annotation1989843/range1989843/1

https://synbiohub.org/public/igem/BBa_K105031/annotation1989843/range1989843/1
type: Range
persistent identity: range1989843
display id: range1989843
version: 1
top level: BBa_K105031
direction: direction/forward
start: 17
end: 23
orientation: inline

BBa_K106699
type: ComponentDefinition
persistent identity: BBa K106699
display id: BBa_K106699
version: 1
was generated by: iGEM to SBOL conversion
description: Gal1 Promoter
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:09:00Z
mutable provenance: Yeast genomic
owned by: james
owned by: myers
mutable description: Galactose-inducible promoter (strong)!
discontinued: false
dominant: false
group u list: _226_
m user id: 0
owner id: 2659
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: None.
star: false
creator: Andrew Horwitz
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K106699_sequence/1

BBa_K105027
type: ComponentDefinition
persistent identity: BBa K105027
display id: BBa_K105027
version: 1
was generated by: iGEM to SBOL conversion
description: cyc100 minimal promoter
created: 2008-10-25T11:00:00Z
modified: 2015-05-08T01:08:52Z
mutable provenance: Amplification from the plasmid pAH3.
owned by: james
owned by: myers
mutable description: This is the core of the CYC1 promoter of ''S. cerevisiae''. This promoter element allows basal transcription. The transcription signal can be modulated by adding operator sites upstream of this BioBrick. In that way activators or repressors can act on the transcription machinery. <br<
discontinued: false
dominant: false
experience: experience/None
group u list: _253_
m user id: 0
owner id: 3313
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: This BioBrick contains the TATA-box and most of the 5'UTR (-139 to -36, relative to the translational start)of the natural CYC1 promoter. A Kozak sequence is not included and has thus to be added!
star: true
creator: Michael Wild
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: TATA box
sequence: https://synbiohub.org/public/igem/BBa_K105027_sequence/1

TATA box
type: SequenceAnnotation
persistent identity: annotation1989774
display id: annotation1989774
version: 1
top level: BBa_K105027
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K105027/annotation1989774/range1989774/1

https://synbiohub.org/public/igem/BBa_K105027/annotation1989774/range1989774/1
type: Range
persistent identity: range1989774
display id: range1989774
version: 1
top level: BBa_K105027
direction: direction/forward
start: 17
end: 23
orientation: inline

BBa_J05217
type: ComponentDefinition
persistent identity: BBa J05217
display id: BBa_J05217
version: 1
was generated by: iGEM to SBOL conversion
description: Regulator for R2-YAP7
creator: Alexander Roth
created: 2005-09-07T11:00:00Z
modified: 2015-08-31T04:08:15Z
discontinued: false
dominant: false
group u list: _34_
m user id: 0
owner id: 412
owning group id: 34
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: binding sites for R3-ATF6 and R4-cMaf
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J05217_sequence/1

https://synbiohub.org/public/igem/BBa_K165035_sequence/1
type: Sequence
persistent identity: BBa K165035 sequence
display id: BBa_K165035_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ctcgagcgaagaccacccacaatgatggtcgaagaccacccacaatgatggtcgaagaccacccacaatgatggtcgaagaccacccacaatgatggtcgaagaccacccacaatgatggtctcgagtactagagctcgagcgatgctgcatcgatgctgcatcgatgctgcattctcgagactagactcgagcagatccgccaggcgtgtatatatagcgtggatggccaggcaactttagtgctgacacatacaggcatatatatatgtgtgcgacgacacatgatcatatggcatgcatgtgctctgtatgtatataaaactcttgttttcttcttttctctaaatattctttccttatacattaggacctttgtagcataaattactatacttctatagacacacaaacacaaatacacacactaaattaataactag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K110014_sequence/1
type: Sequence
persistent identity: BBa K110014 sequence
display id: BBa_K110014_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaattcaaactcagttagtaataataacaggccctcatctattttctcggaaaacttggtggattttgatgatgttaacatggtcgataagaccagactgtttatttttttatttttcagtttcatcattactataccgtttatggtataaattagaaaagttaaagcagcatgttttcatttgaaacaaatactaatgcaataataggtacaccatctactacataattaattgatagtttccttttccgttaagtgcatgcataggacgcccataattttttaagttaaaaaagcatgtatttacctattcgggaaatttacatgacatggatgccataaggaacgaaaatgaaacatgcatgtcagaggaaaaagaacaaagcgagaggaaaaagctgttgcattaccacgtaattttgtatataaatatctgataaataaccattttatttccatccacttcttctgtcgttcatccgttcattgacatcactagagacaccagcgagctatcatcttcatacaacaataactaccaacctta
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J63006_sequence/1
type: Sequence
persistent identity: BBa J63006 sequence
display id: BBa_J63006_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccccattatcttagcctaaaaaaaccttctctttggaactttcagtaatacgcttaactgctcattgctatattgaagtacggattagaagccgccgagcgggtgacagccctccgaaggaagactctcctccgtgcgtcctcgtcttcaccggtcgcgttcctgaaacgcagatgtgcctcgcgccgcactgctccgaacaataaagattctacaatactagcttttatggttatgaagaggaaaaattggcagtaacctggccccacaaaccttcaaatgaacgaatcaaattaacaaccataggatgataatgcgattagttttttagccttatttctggggtaattaatcagcgaagcgatgatttttgatctattaacagatatataaatgcaaaaactgcataaccactttaactaatactttcaacattttcggtttgtattacttcttattcaaatgtaataaaagtatcaacaaaaaattgttaatatacctctatactttaacgtcaaggaggaaactagacccgccgccaccatggag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K586000_sequence/1
type: Sequence
persistent identity: BBa K586000 sequence
display id: BBa_K586000_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgttcagcgaattaattaacttccaaaatgaaggtcatgagtgccaatgccaatgtggtagctgcaaaaataatgaacaatgccaaaaatcatgtagctgcccaacggggtgtaacagcgacgacaaatgcccctgcggtaacaagtctgaagaaaccaagaagtcatgctgctctgggaaatga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K165031_sequence/1
type: Sequence
persistent identity: BBa K165031 sequence
display id: BBa_K165031_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ctgtatataaaaccagtggttatatgtacagactagactgtatataaaaccagtggttatatgtacagactagactgtatataaaaccagtggttatatgtacagactagactgtatataaaaccagtggttatatgtacagactagactcgagcagatccgccaggcgtgtatatatagcgtggatggccaggcaactttagtgctgacacatacaggcatatatatatgtgtgcgacgacacatgatcatatggcatgcatgtgctctgtatgtatataaaactcttgttttcttcttttctctaaatattctttccttatacattaggacctttgcagcataaattactatacttctatagacacacaaacacaaatacacacactaaattaataactag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K110016_sequence/1
type: Sequence
persistent identity: BBa K110016 sequence
display id: BBa_K110016_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: agaacgaactgtagaatagtccggatatgttatccaatgcctgccaaaatgcattgtcacacgctgtagtgctcgaataggtgttgcaatccgtcaatatacgtcttgctctgtgggtaaatgtctcgtgcattaagacaggctagtataaacgagaagaagtatcctgctttgcaatgaaacaatagtatccgctaagaatttaagcaggccaacgtccatactgcttaggacctgtgcctggcaagtcgcagattgaagttttttcaaccatgtaaatttcctaattgggtaagtacatgatgaaacacatatgaagaaaaaagctttcctacatattcaagatttttttctgtgggtggaatactatttaaggagtgctattagtatcttatttgacttcaaagcaatacgataccttttcttttcacctgctctggctataattataattggttacttaaaaatgcaccgttaagaaccatatccaagaatcaaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K165016_sequence/1
type: Sequence
persistent identity: BBa K165016 sequence
display id: BBa_K165016_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cagatccgccaggcgtgtatatatagcgtggatggccaggcaactttagtgctgacacatacaggcatatatatatgtgtgcgacgacacatgatcatatggcatgcatgtgctctgtatgtatataaaactcttgttttcttcttttctctaaatattctttccttatacattaggacctttgcagcataaattactatacttctatagacacgcaaacacaaatacacacactaaattaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K165039_sequence/1
type: Sequence
persistent identity: BBa K165039 sequence
display id: BBa_K165039_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ctcgagcgatgctgcatcgatgctgcatcgatgctgcattctcgagtactagagaagaaatgatggtaaatgaaataggaaatcaaggagcatgaaggcaaaagacaaatataagggtcgaacgaaaaataaagtgaaaagtgttgatatgatgtatttggctttgcggcgccgaaaaaacgagtttacgcaattgcacaatcatgctgactctgtggcggacccgcgctcttgccggcccggcgataacgctgggcgtgaggctgtgcccggcggagttttttgcgcctgcattttccaaggtttaccctgcgctaaggggcgagattggagaagcaataagaatgccggttggggttgcgatgatgacgaccacgacaactggtgtcattatttaagttgccgaaagaacctgagtgcatttgcaacatgagtatactagaagaatgagccaagacttgcgagacgcgagtttgccggtggtgcgaacaatagagcgaccatgaccttgaaggtgagacgcgcataaccgctagagtactttgaagaggaaacagcaatagggttgctaccagtataaatagacaggtacatacaacactggaaatggttgtctgtttgagtacgctttcaattcatttgggtgtgcactttattatgttacaatatggaagggaactttacacttctcctatgcacatatattaattaaagtccaatgctagtagagaaggggggtaacacccctccgcgctcttttccgatttttttctaaaccgtggaatatttcggatatccttttgttgtttccgggtgtacaatatggacttcctcttttctggcaaccaaacccatacatcgggattcctataataccttcgttggtctccctaacatgtaggtggcggaggggagatatacaatagaacagataccagacaagacataatgggctaaacaagactacaccaattacactgcctcattgatggtggtacataacgaactaatactgtagccctagacttgatagccatcatcatatcgaagtttcactaccctttttccatttgccatctattgaagtaataataggcgcatgcaacttcttttctttttttttcttttctctctcccccgttgttgtctcaccatatccgcaatgacaaaaaaaatgatggaagacactaaaggaaaaaattaacgacaaagacagcaccaacagatgtcgttgttccagagctgatgaggggtatcttcgaacacacgaaactttttccttccttcattcacgcacactactctctaatgagcaacggtatacggccttccttccagttacttgaatttgaaataaaaaaagtttgccgctttgctatcaagtataaatagacctgcaattattaatcttttgtttcctcgtcattgttctcgttccctttcttccttgtttctttttctgcacaatatttcaagctataccaagcatacaatcaact
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K165032_sequence/1
type: Sequence
persistent identity: BBa K165032 sequence
display id: BBa_K165032_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ctcgagcgaagaccacccacaatgatggtcgaagaccacccacaatgatggtcgaagaccacccacaatgatggtcgaagaccacccacaatgatggtcgaagaccacccacaatgatggtcgaagaccacccacaatgatggtctcgagactagactcgagcagatccgccaggcgtgtatatatagcgtggatggccaggcaactttagtgctgacacatacaggcatatatatatgtgtgcgacgacacatgatcatatggcatgcatgtgctctgtatgtatataaaactcttgttttcttcttttctctaaatattctttccttatacattaggacctttgtagcataaattactatacttctatagacacacaaacacaaatacacacactaaattaataactag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K105027_sequence/1
type: Sequence
persistent identity: BBa K105027 sequence
display id: BBa_K105027_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gcatgtgctctgtatgtatataaaactcttgttttcttcttttctctaaatattctttccttatacattaggacctttgcagcataaattactatacttctat
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K110015_sequence/1
type: Sequence
persistent identity: BBa K110015 sequence
display id: BBa_K110015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aagaatttattgcgtctgggttcaacactaattatgcgtacgaaagggtgttgacagaggcatttatgggcttaggatgtgttatatccgaggagctttaaaacatcaggatagtgtgcaacgtggcataagctatgtaatcaactactttttattttctatgtacgcatatacatgcattcacgatctgtttcagtgttcagaaaaaaggcacctactgctacggttggcccatacctttattctttgttcttgttacaaacgagtgtgtaattacccaaaaaggaaatttacatgttaaatgaaacccagtaatcagaaaaaacagttaagaaacctaaaatggtagagataaagatacagattcagtggttgctgaaaatcaagtaaaaaaatgaaatagagtcttcatatataaaccgccagaaatgaatta
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K124001_sequence/1
type: Sequence
persistent identity: BBa K124001 sequence
display id: BBa_K124001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: attacccataaggttgtttgtgacggcgtcgtacaagagaacgtgggaactttttaggctcaccaaaaaagaaagaaaaaatacgagttgctgacagaagcctcaagaaaaaaaaaattcttcttcgactatgctggaggcagagatgatcgagccggtagttaactatatatagctaaattggttccatcaccttcttttctggtgtcgctccttctagtgctatttctggcttttcctatttttttttttccatttttctttctctctttctaatatataaattctcttgcattttctatttttctctctatctattctacttgtttattcccttcaaggtttttttttaaggagtacttgtttttagaatatacggtcaacgaactataattaactaaac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K122000_sequence/1
type: Sequence
persistent identity: BBa K122000 sequence
display id: BBa_K122000_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: agccacaatagaagctttctaactgatctatccaaaactgaaaattacattcttgattaggtttatcacaggcaaatgtaatttgtggtattttgccgttcaaaatctgtagaattttctcattggtcacattacaacctgaaaatactttatctacaatcataccattcttataacatgtccccttaatactaggatcaggcatgaacgcatcacagacaaaatcttcttgacaaacgtcacaattgatccctccccatccgttatcacaatgacaggtgtcattttgtgctcttatgggacgatccttattaccgctttcatccggtgatagaccgccacagaggggcagagagcaatcatcacctgcaaacccttctatacactcacatctaccagtgtacgaattgcattcagaaaactgtttgcattcaaaaataggtagcatacaattaaaacatggcgggcatgtatcattgcccttatcttgtgcagttagacgcgaatttttcgaagaagtaccttcaaagaatggggtcttatcttgttttgcaagtaccactgagcaggataataatagaaatgataatatactatagtagagataacgtcgatgacttcccatactgtaattgcttttagttgtgtatttttagtgtgcaagtttctgtaaatcgattaatttttttttctttcctctttttattaaccttaatttttattttagattcctgacttcaactcaagacgcacagatattataacatctgcataataggcatttgcaagaattactcgtgagtaaggaaagagtgaggaactatcgcatacctgcatttaaagatgccgatttgggcgcgaatcctttattttggcttcaccctcatactattatcagggccagaaaaaggaagtgtttccctccttcttgaattgatgttaccctcataaagcacgtggcctcttatcgagaaagaaattaccgtcgctcgtgatttgtttgcaaaaagaacaaaactgaaaaaacccagacacgctcgacttcctgtcttcctattgattgcagcttccaatttcgtcacacaacaaggtcctagcgacggctcacaggttttgtaacaagcaatcgaaggttctggaatggcgggaaagggtttagtaccacatgctatgatgcccactgtgatctccagagcaaagttcgttcgatcgtactgttactctctctctttcaaacagaattgtccgaatcgtgtgacaacaacagcctgttctcacacactcttttcttctaaccaagggggtggtttagtttagtagaacctcgtgaaacttacatttacatatatataaacttgcataaattggtcaatgcaagaaatacatatttggtcttttctaattcgtagtttttcaagttcttagatgctttctttttctcttttttacagatcatcaaggaagtaattatctactttttacaacaaatataaaaca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I766555_sequence/1
type: Sequence
persistent identity: BBa I766555 sequence
display id: BBa_I766555_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cagatccgccaggcgtgtatatatagcgtggatggccaggcaactttagtgctgacacatacaggcatatatatatgtgtgcgacgacacatgatcatatggcatgcatgtgctctgtatgtatataaaactcttgttttcttcttttctctaaatattctttccttatacattaggacctttgcagcataaattactatacttctatagacacacaaacacaaatacacacactaaattaata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K124000_sequence/1
type: Sequence
persistent identity: BBa K124000 sequence
display id: BBa_K124000_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: agcgttggttggtggatcaagcccacgcgtaggcaatcctcgagcagatccgccaggcgtgtatatatagcgtggatggccaggcaactttagtgctgacacatacaggcatatatatatgtgtgcgacgacacatgatcatatggcatgcatgtgctctgtatgtatataaaactcttgttttcttcttttctctaaatattctttccttatacattaggacctttgcagcataaattactatacttctatagacacacaaacacaaatacacacactaaattaata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K165011_sequence/1
type: Sequence
persistent identity: BBa K165011 sequence
display id: BBa_K165011_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ctcgagcgatgctgcatcgatgctgcatcgatgctgcattctcgag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I766558_sequence/1
type: Sequence
persistent identity: BBa I766558 sequence
display id: BBa_I766558_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgaatgatcaaccaaacgccgatatagtcactattggtgataaaattaaaatgcagctattgagaacccatcctaacaacattaaattgtctattaatggaattggtaaagatgccccaactttccaagaatctgctttgattgccgataagttattgagtgtcatgaaggccggcacttacccaaagatttccattttctacaatgacccagtgtcttccctatcttttgaaccatctgaaaaaccgatctttaacgccaagaccattgaacaatccccatcattcggcaaatttgagatcgacacggacgcaaacgttccaagagatttgtttgaatatactttggctaaccaaatgttgacagcaatggctcaaggttatgctgctgaaatttccgccagaagaaacgctatggataacgcttccaagaatgccggtgatatgatcaatcgttactctatcttgtacaacagaacaagacaagctgtcattactaatgaactggttgatattattactggtgcttcctctttgggatgataaaaaaatcaccctgcattgcctctttatttgacgttgtttttgtagaacatgaaacgaattttgacttgatgagacgaagtatatatccaaagaataccttaaatagaaaaggaaagataataaatactaaacactactatatattcaggtaaaatacaaaaattataacatttttaaaactttttttttttgaaagtccttctcgctttaggattttttcccattaagattatgatggtttcatgtatgtgtcagttaaaaaaaatatggctaagtagcaatgaaacgaaccaagaaaatgaaaacaatatatagtgctgttgaaataacaaagacattggtatatatttgtaaaatgtctgttaaatgttttttatctcaggttcttgcttgtctttggtagaagaaattatagtaaacaaacaaacaaacaaacaaaaaaaaaaaaaaaaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I764001_sequence/1
type: Sequence
persistent identity: BBa I764001 sequence
display id: BBa_I764001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: catccgacatccacaggtccattctcacacataagtgccaaacgcaacaggaggggatacactagcagcagaccgttgcaaacgcaggacctccactcctcttctcctcaacacccacttttgccatcgaaaaaccagcccagttattgggcttgattggagctcgctcattccaattccttctattaggctactaacaccatgactttattagcctgtctatcctggcccccctggcgaggttcatgtttgtttatttccgaatgcaacaagctccgcattacacccgaacatcactccagatgagggctttctgagtgtggggtcaaatagtttcatgttccccaaatggcccaaaactgacagtttaaacgctgtcttggaacctaatatgacaaaagcgtgatctcatccaagatgaactaagtttggttcgttgaaatgctaacggccagttggtcaaaaagaaacttccaaaagtcgccataccgtttgtcttgtttggtattgattgacgaatgctcaaaaataatctcattaatgcttagcgcagtctctctatcgcttctgaaccccggtgcacctgtgccgaaacgcaaatggggaaacacccgctttttggatgattatgcattgtctccacattgtatgcttccaagattctggtgggaatactgctgatagcctaacgttcatgatcaaaatttaactgttctaacccctacttgacagcaatatataaacagaaggaagctgccctgtcttaaacctttttttttatcatcattattagcttactttcataattgcgactggttccaattgacaagcttttgattttaacgacttttaacgacaacttgagaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K165001_sequence/1
type: Sequence
persistent identity: BBa K165001 sequence
display id: BBa_K165001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gacaggttatcagcaacaacacagtcatatccattctcaattagctctaccacagtgtgtgaaccaatgtatccagcaccacctgtaaccaaaacaattttagaagtactttcactttgtaactgagctgtcatttatattgaattttcaaaaattcttactttttttttggatggacgcaaagaagtttaataatcatattacatggcattaccaccatatacatatccatatacatatccatatctaatcttacttatatgttgtggaaatgtaaagagccccattatcttagcctaaaaaaaccttctctttggaactttcagtaatacgcttaactgctcattgctatattgaagtacggattagaagccgccgagcgggtgacagccctccgaaggaagactctcctccgtgcgtcctcgtcttcaccggtcgcgttcctgaaacgcagatgtgcctcgcgccctcgaggcctcgagggtaattaatcagcgaagcgatgatttttgatctattaacagatatataaatgcaaaaactgcataaccactttaactaatactttcaacattttcggtttgtattacttcttattcaaatgtaataaaagtatcaacaaaaaattgttaatatacctctatactttaacgtcaaggagaaaaaactata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K105031_sequence/1
type: Sequence
persistent identity: BBa K105031 sequence
display id: BBa_K105031_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gcatgtgctctgtatgtatatgaaactcttgttttcttcttttctctaaatattctttccttatacattaggacctttgcagcataaattactatacttctat
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K105030_sequence/1
type: Sequence
persistent identity: BBa K105030 sequence
display id: BBa_K105030_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gcatgtgctctgtatgtatattaaactcttgttttcttcttttctctaaatattctttccttatacattaggacctttgcagcataaattactatacttctat
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M31201_sequence/1
type: Sequence
persistent identity: BBa M31201 sequence
display id: BBa_M31201_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atttttacagcatcatttatgggtatcctgcaagttaggtgcggaacgtacataacatattacttcaatttgcgttccgcatacggtctgcccaatttgttttgtttgtgatttcctcattcgtcttcctctacagaaacgggtttgatccttcttttatgaatacggcgtgtagttatatatattaagtaatagaagttattgcacttctgtgtaagaagaagatactagggtgatctattctaggcaattttggttgtattgtgttttttacattcggccaaacttgagcaggctgcaattttagtgtcttcggtttttccagcgacagttttacttgtggctcttggtatagtttcttaaaactattaaagttgctaactggtgcagctgtttagatcacaaaagcgtaattaagaataagatctaccaacaagaacagagccaaaatattttcgtccgttatatcaaccatcaaaggaagctttaatcttctcata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J24813_sequence/1
type: Sequence
persistent identity: BBa J24813 sequence
display id: BBa_J24813_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: myers
owned by: james
elements: agcttttcaattcatcttttttttttttgttcttttttttgattccggtttctttgaaatttttttgattcggtaatctccgagcagaaggaagaacgaaggaaggagcacagacttagattggtatatatacgcat
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J63005_sequence/1
type: Sequence
persistent identity: BBa J63005 sequence
display id: BBa_J63005_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aagaaatgatggtaaatgaaataggaaatcaaggagcatgaaggcaaaagacaaatataagggtcgaacgaaaaataaagtgaaaagtgttgatatgatgtatttggctttgcggcgccgaaaaaacgagtttacgcaattgcacaatcatgctgactctgtggcggacccgcgctcttgccggcccggcgataacgctgggcgtgaggctgtgcccggcggagttttttgcgcctgcattttccaaggtttaccctgcgctaaggggcgagattggagaagcaataagaatgccggttggggttgcgatgatgacgaccacgacaactggtgtcattatttaagttgccgaaagaacctgagtgcatttgcaacatgagtatactagaagaatgagccaagacttgcgagacgcgagtttgccggtggtgcgaacaatagagcgaccatgaccttgaaggtgagacgcgcataaccgctagagtactttgaagaggaaacagcaatagggttgctaccagtataaatagacaggtacatacaacactggaaatggttgtctgtttgagtacgctttcaattcatttgggtgtgcactttattatgttacaatatggaagggaactttacacttctcctatgcacatatattaattaaagtccaatgctagtagagaaggggggtaacacccctccgcgctcttttccgatttttttctaaaccgtggaatatttcggatatccttttgttgtttccgggtgtacaatatggacttcctcttttctggcaaccaaacccatacatcgggattcctataataccttcgttggtctccctaacatgtaggtggcggaggggagatatacaatagaacagataccagacaagacataatgggctaaacaagactacaccaattacactgcctcattgatggtggtacataacgaactaatactgtagccctagacttgatagccatcatcatatcgaagtttcactaccctttttccatttgccatctattgaagtaataataggcgcatgcaacttcttttctttttttttcttttctctctcccccgttgttgtctcaccatatccgcaatgacaaaaaaaatgatggaagacactaaaggaaaaaattaacgacaaagacagcaccaacagatgtcgttgttccagagctgatgaggggtatcttcgaacacacgaaactttttccttccttcattcacgcacactactctctaatgagcaacggtatacggccttccttccagttacttgaatttgaaataaaaaaagtttgccgctttgctatcaagtataaatagacctgcaattattaatcttttgtttcctcgtcattgttctcgttccctttcttccttgtttctttttctgcacaatatttcaagctataccaagcatacaatcaact
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K165042_sequence/1
type: Sequence
persistent identity: BBa K165042 sequence
display id: BBa_K165042_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ctcgagcgaagaccacccacaatgatggtcgaagaccacccacaatgatggtcgaagaccacccacaatgatggtcgaagaccacccacaatgatggtcgaagaccacccacaatgatggtctcgagtactagagagctccggatgcaagggttcgaatcccttagctctcattattttttgctttttctcttgaggtcacatgatcgcaaaatggcaaatggcacgtgaagctgtcgatattggggaactgtggtggttggcaaatgactaattaagttagtcaaggcgccatcctcatgaaaactgtgtaacataataaccgaagtgtcgaaaaggtggcaccttgtccaattgaacacgctcgatgaaaaaaataagatatatataaggttaagtaaagcgtctgttagaaaggaagtttttcctttttcttgctctcttgtcttttcatctactatttccttcgtgtaatacagggtcgtcagatacatagatacaattctattacccccatccatac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K110006_sequence/1
type: Sequence
persistent identity: BBa K110006 sequence
display id: BBa_K110006_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tagtgtggatttcaataatttccgaattaggaataaatgcgctaaatagacatcccgttctctttggtaatctgcataattctgatgcaatatccaacaactatttgtgcaattatttaacaaaatccaattaactttcctaattagtccttcaatagaacatctgtattccttttttttatgaacaccttcctaattaggccatcaacgacagtaaattttgccgaatttaatagcttctactgaaaaacagtggaccatgtgaaaagatgcatctcatttatcaaacacataatattcaagtgagccttacttcaattgtattgaagtgcaagaaaaccaaaaagcaacaacaggttttggataagtacatatataagagggccttttgttcccatcaaaaatgttactgttcttacgattcatttacgattcaagaatagttcaaacaagaagattacaaactatcaatttcatacacaatataaacgattaaaagaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K165033_sequence/1
type: Sequence
persistent identity: BBa K165033 sequence
display id: BBa_K165033_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ctcgaggacgccattttaagacgccattttaagacgccattttaactcgagtactagagcagatccgccaggcgtgtatatatagcgtggatggccaggcaactttagtgctgacacatacaggcatatatatatgtgtgcgacgacacatgatcatatggcatgcatgtgctctgtatgtatataaaactcttgttttcttcttttctctaaatattctttccttatacattaggacctttgcagcataaattactatacttctatagacacgcaaacacaaatacacacactaaattaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K165034_sequence/1
type: Sequence
persistent identity: BBa K165034 sequence
display id: BBa_K165034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ctcgagcgatgctgcatcgatgctgcatcgatgctgcattctcgagtactagagctgtatataaaaccagtggttatatgtacagactagactgtatataaaaccagtggttatatgtacagactagactgtatataaaaccagtggttatatgtacagactagactgtatataaaaccagtggttatatgtacagactagactcgagcagatccgccaggcgtgtatatatagcgtggatggccaggcaactttagtgctgacacatacaggcatatatatatgtgtgcgacgacacatgatcatatggcatgcatgtgctctgtatgtatataaaactcttgttttcttcttttctctaaatattctttccttatacattaggacctttgcagcataaattactatacttctatagacacacaaacacaaatacacacactaaattaataactag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K106699_sequence/1
type: Sequence
persistent identity: BBa K106699 sequence
display id: BBa_K106699_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cggtgctatagttttttctccttgacgttaaagtatagaggtatattaacaattttttgttgatacttttattacatttgaataagaagtaatacaaaccgaaaatgttgaaagtattagttaaagtggttatgcagtttttgcatttatatatctgttaatagatcaaaaatcatcgcttcgctgattaattaccccagaaataaggctaaaaaactaatcgcattatcatcctatggttgttaatttgattcgttcatttgaaggtttgtggggccaggttactgccaatttttcctcttcataaccataaaagctagtattgtagaatctttattgttcggagcagtgcggcgcgaggcacatctgcgtttcaggaacgcgaccggtgaagacgaggacgcacggaggagagtcttccttcggagggctgtcacccgctcggcggcttctaatccgtacttcaatatagcaatgagcagttaagcgtattactgaaagttccaaagagaaggtttttttaggctaagataatggggctctttacatttccacaacatataagtaagattagatatggatatgtatatggatatgtatatggtggtaatgccatgtaatatgattattaaacttctttgcgtccatccaaaaaaaaagtaagaatttttgaaaattcaatataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K165037_sequence/1
type: Sequence
persistent identity: BBa K165037 sequence
display id: BBa_K165037_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: attacccataaggttgtttgtgacggcgtcgtacaagagaacgtgggaactttttaggctcaccaaaaaagaaagaaaaaatacgagttgctgacagaagcctcaagaaaaaaaaaattcttcttcgactatgctggaggcagagatgatcgagccggtagttaactatatatagctaaattggttccatcaccttcttttctggtgtcgctccttctagtgctatttctggcttttcctatttttttttttccatttttctttctctctttctaatatataaattctcttgcattttctatttttctctctatctattctacttgtttattcccttcaaggtttttttttaaggagtacttgtttttagaatatacggtcaacgaactataattaactaaac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K165000_sequence/1
type: Sequence
persistent identity: BBa K165000 sequence
display id: BBa_K165000_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: agctccggatgcaagggttcgaatcccttagctctcattattttttgctttttctcttgaggtcacatgatcgcaaaatggcaaatggcacgtgaagctgtcgatattggggaactgtggtggttggcaaatgactaattaagttagtcaaggcgccatcctcatgaaaactgtgtaacataataaccgaagtgtcgaaaaggtggcaccttgtccaattgaacacgctcgatgaaaaaaataagatatatataaggttaagtaaagcgtctgttagaaaggaagtttttcctttttcttgctctcttgtcttttcatctactatttccttcgtgtaatacagggtcgtcagatacatagatacaattctattacccccatccatac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K165036_sequence/1
type: Sequence
persistent identity: BBa K165036 sequence
display id: BBa_K165036_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ctcgagcgaagaccacccacaatgatggtcgaagaccacccacaatgatggtcgaagaccacccacaatgatggtcgaagaccacccacaatgatggtcgaagaccacccacaatgatggtctcgagtactagagctgtatataaaaccagtggttatatgtacagactagactgtatataaaaccagtggttatatgtacagactagactgtatataaaaccagtggttatatgtacagactagactgtatataaaaccagtggttatatgtacagactagactcgagcagatccgccaggcgtgtatatatagcgtggatggccaggcaactttagtgctgacacatacaggcatatatatatgtgtgcgacgacacatgatcatatggcatgcatgtgctctgtatgtatataaaactcttgttttcttcttttctctaaatattctttccttatacattaggacctttgcagcataaattactatacttctatagacacacaaacacaaatacacacactaaattaataactag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K110009_sequence/1
type: Sequence
persistent identity: BBa K110009 sequence
display id: BBa_K110009_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: agaacgaactgtagaatagtccggatatgttatccaatgcctgccaaaatgcattgtcacacgctgtagtgctcgaataggtgttgcaatccgtcaatatacgtcttgctctgtgggtaaatgtctcgtgcattaagacaggctagtataaacgagaagaagtatcctgctttgcaatgaaacaatagtatccgctaagaatttaagcaggccaacgtccatactgcttaggacctgtgcctggcaagtcgcagattgaagttttttcaaccatgtaaatttcctaattgggtaagtacatgatgaaacacatatgaagaaaaaagctttcctacatattcaagatttttttctgtgggtggaatactatttaaggagtgctattagtatcttatttgacttcaaagcaatacgataccttttcttttcacctgctctggctataattataattggttacttaaaaatgcaccgttaagaaccatatccaagaatcaaaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K165043_sequence/1
type: Sequence
persistent identity: BBa K165043 sequence
display id: BBa_K165043_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ctcgagcgatgctgcatcgatgctgcatcgatgctgcattctcgagtactagagagctccggatgcaagggttcgaatcccttagctctcattattttttgctttttctcttgaggtcacatgatcgcaaaatggcaaatggcacgtgaagctgtcgatattggggaactgtggtggttggcaaatgactaattaagttagtcaaggcgccatcctcatgaaaactgtgtaacataataaccgaagtgtcgaaaaggtggcaccttgtccaattgaacacgctcgatgaaaaaaataagatatatataaggttaagtaaagcgtctgttagaaaggaagtttttcctttttcttgctctcttgtcttttcatctactatttccttcgtgtaatacagggtcgtcagatacatagatacaattctattacccccatccatac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I766214_sequence/1
type: Sequence
persistent identity: BBa I766214 sequence
display id: BBa_I766214_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tgttattgtgatagtatctcagcgggatttgtgtagattcacctacagcctttaaaccagcaaagtgaattaccgaatcaattttatattctttgaaaaccttttccagaccttttcggtcacacaaatcaacctcatagaagggaatgtgatgcttggtcaagacctctaacctggctacagaatcataagttgaattcgacaggttatcagcaacaacacagtcatatccattctcaattagctctaccacagtgtgtgaaccaatgtatccagcaccacctgtaaccaaaacaattttagaagtactttcactttgtaactgagctgtcatttatattgaattttcaaaaattcttactttttttttggatggacgcaaagaagtttaataatcatattacatggcattaccaccatatacatatccatatctaatcttacttatatgttgtggaaatgtaaagagccccattatcttagcctaaaaaaaccttctctttggaactttcagtaatacgcttaactgctcattgctatattgaagtacggattagaagccgccgagcgggcgacagccctccgacggaagactctcctccgtgcgtcctcgtcttcaccggtcgcgttcctgaaacgcagatgtgcctcgcgccgcactgctccgaacaataaagattctacaatactagcttttatggttatgaagaggaaaaattggcagtaacctggccccacaaaccttcaaattaacgaatcaaattaacaaccataggatgataatgcgattagttttttagccttatttctggggtaattaatcagcgaagcgatgatttttgatctattaacagatatataaatggaaaagctgcataaccactttaactaatactttcaacattttcagtttgtattacttcttattcaaatgtcataaaagtatcaacaaaaaattgttaatatacctctatactttaacgtcaaggagaaaaaactata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K110005_sequence/1
type: Sequence
persistent identity: BBa K110005 sequence
display id: BBa_K110005_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tgctcattgatgtccctaaaaggtaatgtcgcatagtaagatgtatgtcccatatcaaacgcattcctagaaaaacatgcactgaatgaataagcagttacttcgattactaatcttcacagcataatttttctcttatcttgtaaacctctttctatcttttcataaagtttgtcacttatatttcttcattggtacatcaatgccagcaacgatgtgcgcatctgggcgacgcctgtagtgattgttttcaaggtatcgagccaaactattcatcgttactgtttcaaatattcagttgtttcagtacagagtcgccgtggacctagtgaaacttggtgtctttacagcgcagagacgagggcttatatgtataaaagctgtccttgattctggtgtagtttgaggtgtccttcctatatctgtttttatattctatataatggataattactaccatcacctgcatcaaattccagtaaattcacatattggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K110007_sequence/1
type: Sequence
persistent identity: BBa K110007 sequence
display id: BBa_K110007_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: agctagttgagtagctgaaaaaacatgtttctcaccagcgaatctaacttcggcaccagtcttcttgtcatccaattcaaccaacttagaggtgaagtgcttagattcttgctcgaaatctggatggtggtaatccaaaccaataattcttttcaagttggcgacagtgttcttgatgttggaagtctgcttgttcttaccagtttcacccaagtatctgttctttggaccaaaaccaacaacagatggggtggaacggttagagacttcattaacgacaatgtcgatacctctgtttctagcaacggcaaggacagagttattgttacctaaatctaaaccaaatggagtactcatcttttaaagaaatgttgtttgtcaatgtactttttgcttatggctatcgaggttaaaaataagaatcagaactaactgagttataagaagaaaaatcaagacagagggagtttaaaatggacagtaacttttatcactaaggtt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K149001_sequence/1
type: Sequence
persistent identity: BBa K149001 sequence
display id: BBa_K149001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gatccattattctgggcatccgtgtacaggattctgtcattctagcgtcttctaaggcagtcacaagaggtatttctgttttaaaagattctgatgataaaacgagacaattatcgccacatacattgatgagttttgccggtgaagctggtgacaccgttcaattcgccgagtacattcaagccaatatccaattatactccattagagaagattatgagctctctccacaagcagtatctagttttgttaggcaagaattagccaagtcaattaggtcgagaagaccataccaagtcaacgtattgattggaggctatgacaaaaagaagaacaaaccggaactatatcaaattgactacttgggtactaaagtcgaattaccctatggtgctcatggttactcggggttttacacattctctttactagatcatcattatagacctgatatgactactgaggagggtttagatttattaaaactatgtgtacaagagcttgaaaaaagaatgccaatggacttcaagggcgtcattgttaaaatcgtggataaagatggcataagacaagtagatgacttccaggcacagtgaaagatattcagtgtaatcacacggaaaaggtgattgctaatgatatactaaagtaattatcttcctcgttcataaaattaaataaagttattatagttttttcatccgttataatagcaaatgtatcagaaataagacaatttctcattttttgttggcagagtgagatatcatctgatctattgcaaatgaatcccaatcaatgctgataccgcttcgttataaccaattcgaaaagttgtaattatgttacccgcatgagaatattttgaagaagcaatgtagggctacgatagtacttttgagatggtttattacttgttcaaatgctgtcatctcatgttgttaatgcgctatatttcgcactacgaagctaattttcaacataggtaacacaaagatattagagg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I766200_sequence/1
type: Sequence
persistent identity: BBa I766200 sequence
display id: BBa_I766200_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atccaatatcacctgaccttcatctttatgtttttccactggtgtaatgtactctgttgcatcatcaataagcaactgggacgccaataattcataccaacaatcttttcttaatgagttgtttacaaaccccagaggccctaaacctatgtacctcaaagctcggacatcttttttctctaataactttgatataacttttcctttttgttcttctgcctttccactgtcacaacgagaaagtgattccagatatgcgttataacctccccttgtcaacgcgtctttgtcgtggctactctgattagtatgaaataatgaccttaatggcatcgtattttgttaattggcttgttctatgtcatttgctaaattttctctttatttctttactttgccaattcaagataatcttcccttcccagagagaaaaaaggaaaaatttagctatgaaacctcaataagctttttaatacaccaaagattcaagataagagcatagaacgaactgtagaatagtccggatatgttatccaatgcctgccaaaatgcattgtcacacgctgtagtgctcgaataggtgttgcaatccgtcaatatacgtcttgctctgtgggtaaatgtctcgtgcattaagacaggctagtataaacgagaagaagtatcctgctttgcaatgaaacaatagtatccgctaagaatttaagcaggccaacgtccatactgcttaggacctgtgcctggcaagtcgcagattgaagttttttcaaccatgtaaatttcctaattgggtaagtacatgatgaaacacatatgaagaaaaaagctttcctacatattcaagatttttttctgtgggtggaatactatttaaggagtgctattagtatcttatttgacttcaaagcaatacgataccttttcttttcacctgctctggctataattataattggttacttaaaaatgcaccgttaagaaccatatccaagaatcaaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K165012_sequence/1
type: Sequence
persistent identity: BBa K165012 sequence
display id: BBa_K165012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ctcgagcgaagaccacccacaatgatggtcgaagaccacccacaatgatggtcgaagaccacccacaatgatggtcgaagaccacccacaatgatggtcgaagaccacccacaatgatggtctcgag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K165038_sequence/1
type: Sequence
persistent identity: BBa K165038 sequence
display id: BBa_K165038_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: myers
owned by: james
elements: ctcgagcgaagaccacccacaatgatggtcgaagaccacccacaatgatggtcgaagaccacccacaatgatggtcgaagaccacccacaatgatggtcgaagaccacccacaatgatggtctcgagtactagagaagaaatgatggtaaatgaaataggaaatcaaggagcatgaaggcaaaagacaaatataagggtcgaacgaaaaataaagtgaaaagtgttgatatgatgtatttggctttgcggcgccgaaaaaacgagtttacgcaattgcacaatcatgctgactctgtggcggacccgcgctcttgccggcccggcgataacgctgggcgtgaggctgtgcccggcggagttttttgcgcctgcattttccaaggtttaccctgcgctaaggggcgagattggagaagcaataagaatgccggttggggttgcgatgatgacgaccacgacaactggtgtcattatttaagttgccgaaagaacctgagtgcatttgcaacatgagtatactagaagaatgagccaagacttgcgagacgcgagtttgccggtggtgcgaacaatagagcgaccatgaccttgaaggtgagacgcgcataaccgctagagtactttgaagaggaaacagcaatagggttgctaccagtataaatagacaggtacatacaacactggaaatggttgtctgtttgagtacgctttcaattcatttgggtgtgcactttattatgttacaatatggaagggaactttacacttctcctatgcacatatattaattaaagtccaatgctagtagagaaggggggtaacacccctccgcgctcttttccgatttttttctaaaccgtggaatatttcggatatccttttgttgtttccgggtgtacaatatggacttcctcttttctggcaaccaaacccatacatcgggattcctataataccttcgttggtctccctaacatgtaggtggcggaggggagatatacaatagaacagataccagacaagacataatgggctaaacaagactacaccaattacactgcctcattgatggtggtacataacgaactaatactgtagccctagacttgatagccatcatcatatcgaagtttcactaccctttttccatttgccatctattgaagtaataataggcgcatgcaacttcttttctttttttttcttttctctctcccccgttgttgtctcaccatatccgcaatgacaaaaaaaatgatggaagacactaaaggaaaaaattaacgacaaagacagcaccaacagatgtcgttgttccagagctgatgaggggtatcttcgaacacacgaaactttttccttccttcattcacgcacactactctctaatgagcaacggtatacggccttccttccagttacttgaatttgaaataaaaaaagtttgccgctttgctatcaagtataaatagacctgcaattattaatcttttgtttcctcgtcattgttctcgttccctttcttccttgtttctttttctgcacaatatttcaagctataccaagcatacaatcaact
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K165013_sequence/1
type: Sequence
persistent identity: BBa K165013 sequence
display id: BBa_K165013_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ctcgaggacgccattttaagacgccattttaagacgccattttaactcgag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K105028_sequence/1
type: Sequence
persistent identity: BBa K105028 sequence
display id: BBa_K105028_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gcatgtgctctgtatgtatataacactcttgttttcttcttttctctaaatattctttccttatacattaggacctttgcagcataaattactatacttctat
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K165041_sequence/1
type: Sequence
persistent identity: BBa K165041 sequence
display id: BBa_K165041_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ctcgagcgatgctgcatcgatgctgcatcgatgctgcattctcgagtactagagattacccataaggttgtttgtgacggcgtcgtacaagagaacgtgggaactttttaggctcaccaaaaaagaaagaaaaaatacgagttgctgacagaagcctcaagaaaaaaaaaattcttcttcgactatgctggaggcagagatgatcgagccggtagttaactatatatagctaaattggttccatcaccttcttttctggtgtcgctccttctagtgctatttctggcttttcctatttttttttttccatttttctttctctctttctaatatataaattctcttgcattttctatttttctctctatctattctacttgtttattcccttcaaggtttttttttaaggagtacttgtttttagaatatacggtcaacgaactataattaactaaac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I766556_sequence/1
type: Sequence
persistent identity: BBa I766556 sequence
display id: BBa_I766556_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttaaaacaagaagagggttgactacatcacgatgagggggatcgaagaaatgatggtaaatgaaataggaaatcaaggagcatgaaggcaaaagacaaatataagggtcgaacgaaaaataaagtgaaaagtgttgatatgatgtatttggctttgcggcgccgaaaaaacgagtttacgcaattgcacaatcatgctgactctgtggcggacccgcgctcttgccggcccggcgataacgctgggcgtgaggctgtgcccggcggagttttttgcgcctgcattttccaaggtttaccctgcgctaaggggcgagattggagaagcaataagaatgccggttggggttgcgatgatgacgaccacgacaactggtgtcattatttaagttgccgaaagaacctgagtgcatttgcaacatgagtatactagaagaatgagccaagacttgcgagacgcgagtttgccggtggtgcgaacaatagagcgaccatgaccttgaaggtgagacgcgcataaccgctagagtactttgaagaggaaacagcaatagggttgctaccagtataaatagacaggtacatacaacactggaaatggttgtctgtttgagtacgctttcaattcatttgggtgtgcactttattatgttacaatatggaagggaactttacacttctcctatgcacatatattaattaaagtccaatgctagtagagaaggggggtaacacccctccgcgctcttttccgatttttttctaaaccgtggaatatttcggatatccttttgttgtttccgggtgtacaatatggacttcctcttttctggcaaccaaacccatacatcgggattcctataataccttcgttggtctccctaacatgtaggtggcggaggggagatatacaatagaacagataccagacaagacataatgggctaaacaagactacaccaattacactgcctcattgatggtggtacataacgaactaatactgtagccctagacttgatagccatcatcatatcgaagtttcactaccctttttccatttgccatctattgaagtaataataggcgcatgcaacttcttttctttttttttcttttctctctcccccgttgttgtctcaccatatccgcaatgacaaaaaaatgatggaagacactaaaggaaaaaattaacgacaaagacagcaccaacagatgtcgttgttccagagctgatgaggggtatctcgaagcacacgaaactttttccttccttcattcacgcacactactctctaatgagcaacggtatacggccttccttccagttacttgaatttgaaataaaaaaaagtttgctgtcttgctatcaagtataaatagacctgcaattattaatcttttgtttcctcgtcattgttctcgttccctttcttccttgtttctttttctgcacaatatttcaagctataccaagcatacaatcaactatctcatataca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K124002_sequence/1
type: Sequence
persistent identity: BBa K124002 sequence
display id: BBa_K124002_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcagttcgagtttatcattatcaatactgccatttcaaagaatacgtaaataattaatagtagtgattttcctaactttatttagtcaaaaaattagccttttaattctgctgtaacccgtacatgcccaaaatagggggcgggttacacagaatatataacatcgtaggtgtctgggtgaacagtttattcctggcatccactaaatataatggagcccgctttttaagctggcatccagaaaaaaaaagaatcccagcaccaaaatattgttttcttcaccaaccatcagttcataggtccattctcttagcgcaactacagagaacaggggcacaaacaggcaaaaaacgggcacaacctcaatggagtgatgcaacctgcctggagtaaatgatgacacaaggcaattgacccacgcatgtatctatctcattttcttacaccttctattaccttctgctctctctgatttggaaaaagctgaaaaaaaaggttgaaaccagttccctgaaattattcccctacttgactaataagtatataaagacggtaggtattgattgtaattctgtaaatctatttcttaaacttcttaaattctacttttatagttagtcttttttttagttttaaaacaccaagaacttagtttcgaataaacacacataaacaaacaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K105029_sequence/1
type: Sequence
persistent identity: BBa K105029 sequence
display id: BBa_K105029_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gcatgtgctctgtatgtatatagaactcttgttttcttcttttctctaaatattctttccttatacattaggacctttgcagcataaattactatacttctat
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K110004_sequence/1
type: Sequence
persistent identity: BBa K110004 sequence
display id: BBa_K110004_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tgaaaattttgatagtattttgcctttcctacacaaatttaattgcctctaaagttaaaaagcaataaaatagaatatgtgaatactgcacaaaatgaatacttgggaattcagaaattattcttaccttaagcatccactcatatacatatatatatatatatatgtcttttcaaaagacttctgcccaaattgtggagagaactctgcctctccgtgataacggccaaatgaaacgcatccaatgatgcaattattgacaaagtttcaaagtttccgccattattccttcatttacacattgatagtcgagaagtttcctaattagtgtcacaatgacagagaaaattgtaccagcgcttctaacgaaaattagcacccatacccagagttcataaatctggcaatgtggtacaataatataggattggtaacatttcaattgtaaaatgtttaacgtttagagaaaaggggagccagaacgcttctggtggtgtaaat
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K165015_sequence/1
type: Sequence
persistent identity: BBa K165015 sequence
display id: BBa_K165015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aagaaatgatggtaaatgaaataggaaatcaaggagcatgaaggcaaaagacaaatataagggtcgaacgaaaaataaagtgaaaagtgttgatatgatgtatttggctttgcggcgccgaaaaaacgagtttacgcaattgcacaatcatgctgactctgtggcggacccgcgctcttgccggcccggcgataacgctgggcgtgaggctgtgcccggcggagttttttgcgcctgcattttccaaggtttaccctgcgctaaggggcgagattggagaagcaataagaatgccggttggggttgcgatgatgacgaccacgacaactggtgtcattatttaagttgccgaaagaacctgagtgcatttgcaacatgagtatactagaagaatgagccaagacttgcgagacgcgagtttgccggtggtgcgaacaatagagcgaccatgaccttgaaggtgagacgcgcataaccgctagagtactttgaagaggaaacagcaatagggttgctaccagtataaatagacaggtacatacaacactggaaatggttgtctgtttgagtacgctttcaattcatttgggtgtgcactttattatgttacaatatggaagggaactttacacttctcctatgcacatatattaattaaagtccaatgctagtagagaaggggggtaacacccctccgcgctcttttccgatttttttctaaaccgtggaatatttcggatatccttttgttgtttccgggtgtacaatatggacttcctcttttctggcaaccaaacccatacatcgggattcctataataccttcgttggtctccctaacatgtaggtggcggaggggagatatacaatagaacagataccagacaagacataatgggctaaacaagactacaccaattacactgcctcattgatggtggtacataacgaactaatactgtagccctagacttgatagccatcatcatatcgaagtttcactaccctttttccatttgccatctattgaagtaataataggcgcatgcaacttcttttctttttttttcttttctctctcccccgttgttgtctcaccatatccgcaatgacaaaaaaaatgatggaagacactaaaggaaaaaattaacgacaaagacagcaccaacagatgtcgttgttccagagctgatgaggggtatcttcgaacacacgaaactttttccttccttcattcacgcacactactctctaatgagcaacggtatacggccttccttccagttacttgaatttgaaataaaaaaagtttgccgctttgctatcaagtataaatagacctgcaattattaatcttttgtttcctcgtcattgttctcgttccctttcttccttgtttctttttctgcacaatatttcaagctataccaagcatacaatcaact
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K165040_sequence/1
type: Sequence
persistent identity: BBa K165040 sequence
display id: BBa_K165040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ctcgagcgaagaccacccacaatgatggtcgaagaccacccacaatgatggtcgaagaccacccacaatgatggtcgaagaccacccacaatgatggtcgaagaccacccacaatgatggtctcgagtactagagattacccataaggttgtttgtgacggcgtcgtacaagagaacgtgggaactttttaggctcaccaaaaaagaaagaaaaaatacgagttgctgacagaagcctcaagaaaaaaaaaattcttcttcgactatgctggaggcagagatgatcgagccggtagttaactatatatagctaaattggttccatcaccttcttttctggtgtcgctccttctagtgctatttctggcttttcctatttttttttttccatttttctttctctctttctaatatataaattctcttgcattttctatttttctctctatctattctacttgtttattcccttcaaggtttttttttaaggagtacttgtttttagaatatacggtcaacgaactataattaactaaac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K165030_sequence/1
type: Sequence
persistent identity: BBa K165030 sequence
display id: BBa_K165030_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ctcgagcgatgctgcatcgatgctgcatcgatgctgcattctcgagactagactcgagcagatccgccaggcgtgtatatatagcgtggatggccaggcaactttagtgctgacacatacaggcatatatatatgtgtgcgacgacacatgatcatatggcatgcatgtgctctgtatgtatataaaactcttgttttcttcttttctctaaatattctttccttatacattaggacctttgtagcataaattactatacttctatagacacacaaacacaaatacacacactaaattaataactag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I766557_sequence/1
type: Sequence
persistent identity: BBa I766557 sequence
display id: BBa_I766557_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cacgaagtgactgacagaatactgacatcagctgatttctcatagagctgtttctctgataacacgttgtttgaacatcgacaagatgaaaatctagaagtatcaagtttcctttaaagggatatataacagattctaaaactgacagaaatatttcgagtgaagaagaagcgttaaatattggatctttccgcagttctactctgatacatttttgaagtaggagagtcatttagaaggcgtattgctcaatagtagaaagcaggcctgtgcacatgaattaattaaaaaatataaaggtagtgattagacgacacatgtccataggtaacctgtcataattttgaacaatttcccttcttttcttttttttttttgggtgcggcgatatgtagcttgttaatttacacatcatgtacttttctgcatcaaaatatgaaaggcgatagtagctaaagaaaataccgagaatttcctcgaaaagttgacgacaaaagaaaggcataaaaaagtaatttgaaaatattttaaaactgttttaacccatctagcatccgcgctaaaaaaggaagatacaggatacagcggaaacaacttttaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K110008_sequence/1
type: Sequence
persistent identity: BBa K110008 sequence
display id: BBa_K110008_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aagaatttattgcgtctgggttcaacactaattatgcgtacgaaagggtgttgacagaggcatttatgggcttaggatgtgttatatccgaggagctttaaaacatcaggatagtgtgcaacgtggcataagctatgtaatcaactactttttattttctatgtacgcatatacatgcattcacgatctgtttcagtgttcagaaaaaaggcacctactgctacggttggcccatacctttattctttgttcttgttacaaacgagtgtgtaattacccaaaaaggaaatttacatgttaaatgaaacccagtaatcagaaaaaacagttaagaaacctaaaatggtagagataaagatacagattcagtggttgctgaaaatcaagtaaaaaaatgaaatagagtcttcatatataaaccgccagaaatgaattaatgagagggatctgtaactgtttctcggataaaaccaaaataagtacaaagccatcgaatagaaa
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z