//ribosome/eukaryote
type: Collection
persistent identity: ribosome eukaryote
display id: ribosome_eukaryote
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
member: //ribosome/eukaryote/yeast

//ribosome/prokaryote/pputida
type: Collection
persistent identity: ribosome prokaryote pputida
display id: ribosome_prokaryote_pputida
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
member: BBa_I723019

//ribosome/prokaryote/bsubtilis
type: Collection
persistent identity: ribosome prokaryote bsubtilis
display id: ribosome_prokaryote_bsubtilis
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
member: BBa_K090505
member: BBa_K090506
member: RBS-GsiB
member: RBS-spoVG
member: R38
member: R37

//ribosome/prokaryote/ecoli
type: Collection
persistent identity: ribosome prokaryote ecoli
display id: ribosome_prokaryote_ecoli
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
member: BBa_I742163
member: BBa_J59001
member: BBa_B0041
member: BBa_M13501
member: BBa_J61139
member: BBa_J61109
member: BBa_B2017
member: Lock3
member: BBa_B0039
member: creA RBS
member: BBa_J61127
member: BBa_B0032
member: BBa_J61106
member: BBa_J34803
member: BBa_J61118
member: BBa_M13508
member: creD RBS
member: BBa_J61102
member: BBa_B2040
member: BBa_J61132
member: BBa_J61115
member: BBa_J61145
member: BBa_J61103
member: BBa_J61133
member: BBa_J61120
member: BBa_B2022
member: BBa_K103015
member: T7 RBS
member: BBa_B0038
member: BBa_J61147
member: BBa_J61121
member: BBa_J61117
member: BBa_M13502
member: BBa_J64808
member: BBa_J15001
member: BBa_K150005
member: BBa_J56013
member: BBa_J23080
member: BBa_J61114
member: BBa_J61144
member: BBa_J61110
member: BBa_J61140
member: BBa_J61123
member: BBa_J61136
member: BBa_M13504
member: BBa_K1895001
member: BBa_J61119
member: T7 0.4
member: BBa_B0034
member: BBa_B0064
member: BBa_J61108
member: BBa_J61138
member: BBa_J61112
member: BBa_J61125
member: BBa_J61142
member: BBa_M13510
member: BBa_J61134
member: BBa_K118012
member: BBa_J34801
member: BBa_K1847000
member: nickname
member: BBa_M13506
member: BBa_I11010
member: BBa_J61104
member: BBa_J61113
member: BBa_K1675006
member: BBa_J34810
member: BBa_J61143
member: trkD
member: BBa_K819017
member: BBa_M13503
member: BBa_J56016

//ribosome/prokaryote/salmonella
type: Collection
persistent identity: ribosome prokaryote salmonella
display id: ribosome_prokaryote_salmonella
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
member: BBa_J64024
member: BBa_J64031
member: BBa_J64011
member: BBa_J64030
member: BBa_J64018
member: BBa_J64022
member: BBa_J64020
member: BBa_J64026
member: BBa_J64013
member: BBa_J64028
member: BBa_J64015

//ribosome/prokaryote/sespanaensis
type: Collection
persistent identity: ribosome prokaryote sespanaensis
display id: ribosome_prokaryote_sespanaensis
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
member: BBa_I742145
member: BBa_I742146

//ribosome/eukaryote/yeast
type: Collection
persistent identity: ribosome eukaryote yeast
display id: ribosome_eukaryote_yeast
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
member: Kozak & st
member: BBa_K165002

//ribosome/prokaryote/bcepacia
type: Collection
persistent identity: ribosome prokaryote bcepacia
display id: ribosome_prokaryote_bcepacia
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
member: BBa_I723012
member: BBa_I723014

//ribosome/prokaryote/pananatis
type: Collection
persistent identity: ribosome prokaryote pananatis
display id: ribosome_prokaryote_pananatis
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
member: BBa_I742150
member: BBa_I742156
member: BBa_I742159
member: BBa_I742153

//ribosome/prokaryote
type: Collection
persistent identity: ribosome prokaryote
display id: ribosome_prokaryote
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
member: //ribosome/prokaryote/ecoli
member: //ribosome/prokaryote/pananatis
member: //ribosome/prokaryote/sespanaensis
member: //ribosome/prokaryote/bsubtilis
member: //ribosome/prokaryote/bcepacia
member: //ribosome/prokaryote/pputida
member: //ribosome/prokaryote/salmonella
member: PnisA
member: //ribosome/prokaryote/custom

//ribosome
type: Collection
persistent identity: ribosome
display id: ribosome
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
member: //ribosome/eukaryote
member: //ribosome/prokaryote

//ribosome/prokaryote/custom
type: Collection
persistent identity: ribosome prokaryote custom
display id: ribosome_prokaryote_custom
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
member: BBa_B0072
member: BBa_B0077
member: BBa_B0071
member: BBa_B0078
member: BBa_B0076
member: BBa_B0073
member: BBa_B0074
member: BBa_B0075
member: BBa_B0036
member: BBa_B0070
member: BBa_B0037

BBa_K2012000
type: ComponentDefinition
persistent identity: BBa K2012000
display id: BBa_K2012000
version: 1
was generated by: iGEM to SBOL conversion
description: c-di-GMP tandem riboswitche bc3-5
creator: Pan Chu
created: 2016-08-04T11:00:00Z
modified: 2016-10-09T01:16:18Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2479_
m user id: 25212
owner id: 25212
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Three complete c-di-GMP riboswitches (Bc3, Bc4 and Bc5 RNA) with similar structures, which are arranged in tandem to constitute a triple-tandem (Bc3-5 RNA) riboswitch in the 5′-UTR of the cspABCDE mRNA in Bacillus thuringiensis subsp. chinensis CT-43.
mutable notes: We designed the sequence of bc3-5 by add perfix and suffix which are belong to BioBrick RFC 10.
mutable provenance: From Bacillus thuringiensis subsp. chinensis CT-43 In the CT-43 genome19,20 (GenBank accession: NC_017208.1), five type I c-di-GMP riboswitches named Bc1, Bc2, Bc3, Bc4 and Bc5 RNA (Supplementary Tables S1???S5) were annotated in the Rfam database (http://rfam.sanger.ac.uk/search). Zhou, H., et al. (2016). "Characterization of a natural triple-tandem c-di-GMP riboswitch and application of the riboswitch-based dual-fluorescence reporter." Sci Rep 6: 20871.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Inverter
sequence: https://synbiohub.org/public/igem/BBa_K2012000_sequence/1

RBS
type: ComponentDefinition
persistent identity: BBa J29048
display id: BBa_J29048
version: 1
was generated by: iGEM to SBOL conversion
description: RBS
creator: Maiko Furubayashi
created: 2006-10-19T11:00:00Z
modified: 2015-08-31T04:08:45Z
discontinued: false
dominant: false
experience: experience/None
group u list: _59_
m user id: 0
owner id: 846
owning group id: 59
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: strong rbs
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J29048_sequence/1

T7 RBS
type: ComponentDefinition
persistent identity: BBa K1362090
display id: BBa_K1362090
version: 1
was generated by: iGEM to SBOL conversion
description: strong T7 RBS
creator: Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena B??scher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Sch??fer, Carolin Schmelas, Silvan Schmitz, Max Waldha
created: 2014-10-02T11:00:00Z
modified: 2015-05-08T01:10:04Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1738_
m user id: 0
owner id: 22830
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: RFC10 compatible strong RBS derived from the T7 phage gene 10a (major capsid protein)[1]. When assembled to a coding part with the A of the start codon being part of the XbaI site, the RBS will be shifted one bp downstream compared to the native sequence. The sequence was successfully used by the iGEM team Heidelberg 2014 for the expression of many proteins in E.coli. 1. Olinss, P. & Rangwala, S. H. Derived from Bacteriophage T7 mRNA Acts ELS an Enhancer of Translation of the lac2 Gene in. 16973???16976 (1989).
mutable notes: The 18 bp including the XbaI that can be found upstream of the presumably important part of the RBS were included into the sequence just to make sure it works. However to fully comply with RFC10 a G was inserted behind the XbaI site.
mutable provenance: synthesized as found in the T7 genome and several commercial expression plasmids.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: t7 RBS
sequence annotation: Shine-Dalgarno
sequence: https://synbiohub.org/public/igem/BBa_K1362090_sequence/1

t7 RBS
type: SequenceAnnotation
persistent identity: annotation2393795
display id: annotation2393795
version: 1
top level: T7 RBS
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1362090/annotation2393795/range2393795/1

https://synbiohub.org/public/igem/BBa_K1362090/annotation2393795/range2393795/1
type: Range
persistent identity: range2393795
display id: range2393795
version: 1
direction: direction/forward
top level: T7 RBS
start: 11
end: 28
orientation: inline

Shine-Dalgarno
type: SequenceAnnotation
persistent identity: annotation2393796
display id: annotation2393796
version: 1
top level: T7 RBS
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1362090/annotation2393796/range2393796/1

https://synbiohub.org/public/igem/BBa_K1362090/annotation2393796/range2393796/1
type: Range
persistent identity: range2393796
display id: range2393796
version: 1
direction: direction/forward
top level: T7 RBS
start: 21
end: 28
orientation: inline

BBa_I742150
type: ComponentDefinition
persistent identity: BBa I742150
display id: BBa_I742150
version: 1
was generated by: iGEM to SBOL conversion
description: crtE native rbs
creator: Chris French
created: 2007-10-23T11:00:00Z
modified: 2015-08-31T04:08:03Z
discontinued: false
dominant: false
experience: experience/None
group u list: _123_
m user id: 0
owner id: 837
owning group id: 163
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is the native ribosome binding site of crtE (geranylgeranyl pyrophosphate synthase) from Pantoea ananatis (formerly Erwinia uredovora) DSMZ 30080 (ATCC 19321) Accession: D90087.
mutable notes: This is a virtual part to be added, blunt ended, to the coding sequence.
mutable provenance: Pantoea ananatis (formerly Erwinia uredovora) DSMZ 30080 (ATCC 19321) Accession: D90087.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_I742150_sequence/1

BBa_M13502
type: ComponentDefinition
persistent identity: BBa M13502
display id: BBa_M13502
version: 1
was generated by: iGEM to SBOL conversion
description: M13KO7 gene II RBS
creator: Natalie Kuldell
created: 2006-12-14T12:00:00Z
modified: 2015-05-08T01:13:56Z
discontinued: false
dominant: false
experience: experience/None
group u list: _45_
m user id: 0
owner id: 314
owning group id: 1
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This RBS is part of the bacteriophage M13 genome, initiating translation of the gene II message (BBa_M13002). The part aligns with base pairs 8252-8267 in M13K07 genome. It was identified as the RBS for gene II message by Wezenbeek et al in Gene (1980) 11: 129-148
mutable notes: none
mutable provenance: New England Biolabs M13K07. Sequenced in 2002 by L. Panganaban
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_M13502_sequence/1

BBa_J44001
type: ComponentDefinition
persistent identity: BBa J44001
display id: BBa_J44001
version: 1
was generated by: iGEM to SBOL conversion
description: Reverse RBS (RBSrev) -- corresponds to BBa_B0030
creator: Todd Eckdahl
created: 2006-08-01T11:00:00Z
modified: 2015-08-31T04:08:48Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _61_71_
m user id: 0
owner id: 606
owning group id: 61
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: Reverse version of RBS BBa_B0030
mutable notes: Repeats in oligos caused unusual products during cloning
mutable provenance: Cloned from synthetic oligos
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: Reverse RBS
sequence: https://synbiohub.org/public/igem/BBa_J44001_sequence/1

Reverse RBS
type: SequenceAnnotation
persistent identity: annotation1893199
display id: annotation1893199
version: 1
top level: BBa_J44001
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_J44001/annotation1893199/range1893199/1

https://synbiohub.org/public/igem/BBa_J44001/annotation1893199/range1893199/1
type: Range
persistent identity: range1893199
display id: range1893199
version: 1
direction: direction/reverse
top level: BBa_J44001
start: 1
end: 15
orientation: inline

BBa_M13507
type: ComponentDefinition
persistent identity: BBa M13507
display id: BBa_M13507
version: 1
was generated by: iGEM to SBOL conversion
description: M13KO7 gene VII RBS
creator: Natalie Kuldell
created: 2006-12-14T12:00:00Z
modified: 2015-05-08T01:13:56Z
discontinued: false
dominant: true
experience: experience/None
group u list: _45_
m user id: 0
owner id: 314
owning group id: 1
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This RBS is part of the bacteriophage M13 genome, initiating translation of the gene VII message (BBa_M13007). The part aligns with base pairs 1092-1107 in M13K07 genome. It was identified as the RBS for gene VII message by Wezenbeek et al in Gene (1980) 11: 129-148
mutable notes: New England Biolabs M13K07. Sequenced in 2002 by L. Panganaban
mutable provenance: New England Biolabs M13K07. Sequenced in 2002 by L. Panganaban
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_M13507_sequence/1

BBa_J61109
type: ComponentDefinition
persistent identity: BBa J61109
display id: BBa_J61109
version: 1
was generated by: iGEM to SBOL conversion
description: Ribosome Binding Site Family Member
creator: John Anderson
created: 2007-04-23T11:00:00Z
modified: 2015-08-31T02:03:00Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable description: {{JCA_Arkin_RBSFamily}}
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J61109_sequence/1

BBa_K090506
type: ComponentDefinition
persistent identity: BBa K090506
display id: BBa_K090506
version: 1
was generated by: iGEM to SBOL conversion
description: ''Bacillus subtilis'' weak RBS
creator: Daniel Goodman
created: 2008-10-27T12:00:00Z
modified: 2015-05-08T01:08:37Z
discontinued: false
dominant: false
experience: experience/None
group u list: _188_
m user id: 0
owner id: 3501
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is the weak version of the consensus RBS for Bacillus subtilis. The sequence is present in front of some B. subtilis operons, but is expected to give weaker expression than the consensus sequence. The consensus sequence AAAGGAGG (BBa_K090505) was changed to AGAGGTGG for this part.
mutable notes: The part must be 8 nucleotides away from the first methionine of the coding sequence, and so we added 'TGT' downstream of the actual binding sequence: AGAGGTGG. This, combined with the truncated non-CDS BioBrick prefix scar, gives exactly 8 'spacer bases' between the 3' end of the RBS and the start of the coding sequence.
mutable provenance: This part was synthesized directly.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: B. subtilis weak RBS
sequence: https://synbiohub.org/public/igem/BBa_K090506_sequence/1

B. subtilis weak RBS
type: SequenceAnnotation
persistent identity: annotation1992815
display id: annotation1992815
version: 1
top level: BBa_K090506
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K090506/annotation1992815/range1992815/1

https://synbiohub.org/public/igem/BBa_K090506/annotation1992815/range1992815/1
type: Range
persistent identity: range1992815
display id: range1992815
version: 1
direction: direction/forward
top level: BBa_K090506
start: 1
end: 8
orientation: inline

BBa_K121016
type: ComponentDefinition
persistent identity: BBa K121016
display id: BBa_K121016
version: 1
was generated by: iGEM to SBOL conversion
description: Tokyo Standard prefix suffix RBS
creator: Shotaro Ayukawa
created: 2008-10-25T11:00:00Z
modified: 2015-05-08T01:09:41Z
discontinued: false
dominant: false
experience: experience/None
group u list: _220_
m user id: 0
owner id: 765
owning group id: 76
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Restriction sites of Sal I, Bam HI, Bgl II, and Mlu I in front of strong RBS
mutable notes: We designed new prefix(Sal I and Bam HI) and suffix(Bgl II and Mul I) for insertions of promoters and regulator binding sites in front of RBS.
mutable provenance: RBS is from BBa_0030
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: Mlu I
sequence annotation: B0030
sequence annotation: Bgl II
sequence annotation: Bam HI
sequence annotation: Sal I
sequence: https://synbiohub.org/public/igem/BBa_K121016_sequence/1

Mlu I
type: SequenceAnnotation
persistent identity: annotation1988250
display id: annotation1988250
version: 1
top level: BBa_K121016
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K121016/annotation1988250/range1988250/1

https://synbiohub.org/public/igem/BBa_K121016/annotation1988250/range1988250/1
type: Range
persistent identity: range1988250
display id: range1988250
version: 1
direction: direction/forward
top level: BBa_K121016
start: 41
end: 46
orientation: inline

B0030
type: SequenceAnnotation
persistent identity: annotation1988216
display id: annotation1988216
version: 1
top level: BBa_K121016
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K121016/annotation1988216/range1988216/1

https://synbiohub.org/public/igem/BBa_K121016/annotation1988216/range1988216/1
type: Range
persistent identity: range1988216
display id: range1988216
version: 1
direction: direction/forward
top level: BBa_K121016
start: 50
end: 64
orientation: inline

Bgl II
type: SequenceAnnotation
persistent identity: annotation1988248
display id: annotation1988248
version: 1
top level: BBa_K121016
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K121016/annotation1988248/range1988248/1

https://synbiohub.org/public/igem/BBa_K121016/annotation1988248/range1988248/1
type: Range
persistent identity: range1988248
display id: range1988248
version: 1
direction: direction/forward
top level: BBa_K121016
start: 26
end: 31
orientation: inline

Bam HI
type: SequenceAnnotation
persistent identity: annotation1988247
display id: annotation1988247
version: 1
top level: BBa_K121016
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K121016/annotation1988247/range1988247/1

https://synbiohub.org/public/igem/BBa_K121016/annotation1988247/range1988247/1
type: Range
persistent identity: range1988247
display id: range1988247
version: 1
direction: direction/forward
top level: BBa_K121016
start: 15
end: 20
orientation: inline

Sal I
type: SequenceAnnotation
persistent identity: annotation1988246
display id: annotation1988246
version: 1
top level: BBa_K121016
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K121016/annotation1988246/range1988246/1

https://synbiohub.org/public/igem/BBa_K121016/annotation1988246/range1988246/1
type: Range
persistent identity: range1988246
display id: range1988246
version: 1
direction: direction/forward
top level: BBa_K121016
start: 1
end: 6
orientation: inline

BBa_J61116
type: ComponentDefinition
persistent identity: BBa J61116
display id: BBa_J61116
version: 1
was generated by: iGEM to SBOL conversion
description: Ribosome Binding Site Family Member
creator: John Anderson
created: 2007-04-23T11:00:00Z
modified: 2015-08-31T02:03:00Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable description: {{JCA_Arkin_RBSFamily}}
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J61116_sequence/1

BBa_K1390002
type: ComponentDefinition
persistent identity: BBa K1390002
display id: BBa_K1390002
version: 1
was generated by: iGEM to SBOL conversion
description: mmoC
created: 2014-07-02T11:00:00Z
modified: 2015-05-08T01:10:13Z
mutable provenance: Amplified from the genomic DNA of Methylococcus capsulatus bath
owned by: james
owned by: myers
mutable description: mmoC is one subunit of the mmo from Methylococcus capsulatus bath
discontinued: false
dominant: false
group u list: _1767_
m user id: 0
owner id: 17096
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: the brick is constructed in the RCF10 standard
star: false
creator: Stefan D??bel
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence: https://synbiohub.org/public/igem/BBa_K1390002_sequence/1

creD RBS
type: ComponentDefinition
persistent identity: BBa J64609
display id: BBa_J64609
version: 1
was generated by: iGEM to SBOL conversion
description: creD RBS
creator: Ryan Ritterson
created: 2007-03-19T12:00:00Z
modified: 2015-05-08T01:08:17Z
discontinued: false
dominant: false
experience: experience/None
group u list: _98_
m user id: 0
owner id: 1422
owning group id: 98
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: The ribosomal binding site of the creD gene in the E Coli creD operon.
mutable notes: natural sequence, no design.
mutable provenance: E Coli Strain K12
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J64609_sequence/1

Lock3
type: ComponentDefinition
persistent identity: BBa J01080
display id: BBa_J01080
version: 1
was generated by: iGEM to SBOL conversion
description: Lock3
creator: Golden Bear
created: 2005-11-29T12:00:00Z
modified: 2015-08-31T04:08:12Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _13_
m user id: 0
owner id: 395
owning group id: 13
sample status: In stock
status: status/Available
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000834
role: partType/RNA
sequence: https://synbiohub.org/public/igem/BBa_J01080_sequence/1

BBa_J61142
type: ComponentDefinition
persistent identity: BBa J61142
display id: BBa_J61142
version: 1
was generated by: iGEM to SBOL conversion
description: {rbs1} Library Member in BBb
creator: John Anderson
created: 2007-09-03T11:00:00Z
modified: 2015-08-31T01:56:25Z
discontinued: false
dominant: false
experience: experience/None
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: {{BBbRBS1Family}}
mutable notes: {{BerkiGEM2007-Biobrick2.0Intro}}
mutable provenance: Synthetic
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J61142_sequence/1

T7 0.3
type: ComponentDefinition
persistent identity: BBa B2001
display id: BBa_B2001
version: 1
was generated by: iGEM to SBOL conversion
description: T7 RBS 0.3
creator: Heather Keller
created: 2006-09-26T11:00:00Z
modified: 2015-08-31T04:07:21Z
discontinued: false
dominant: true
experience: experience/None
group u list: _11_
m user id: 0
owner id: 571
owning group id: 10
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Test RBS from T7 that may be used in E.coli
mutable notes: None
mutable provenance: T7+ genome RBS upstream of gene 0.3
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: SD region
sequence: https://synbiohub.org/public/igem/BBa_B2001_sequence/1

SD region
type: SequenceAnnotation
persistent identity: annotation1902503
display id: annotation1902503
version: 1
top level: T7 0.3
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_B2001/annotation1902503/range1902503/1

https://synbiohub.org/public/igem/BBa_B2001/annotation1902503/range1902503/1
type: Range
persistent identity: range1902503
display id: range1902503
version: 1
direction: direction/forward
top level: T7 0.3
start: 8
end: 12
orientation: inline

BBa_M13503
type: ComponentDefinition
persistent identity: BBa M13503
display id: BBa_M13503
version: 1
was generated by: iGEM to SBOL conversion
description: M13K07 gene III RBS
creator: Natalie Kuldell
created: 2006-12-14T12:00:00Z
modified: 2015-05-08T01:13:56Z
discontinued: false
dominant: false
experience: experience/None
group u list: _45_
m user id: 0
owner id: 314
owning group id: 1
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This RBS is part of the bacteriophage M13 genome, initiating translation of the gene III message (BBa_M13003). The part aligns with base pairs 1563-1578 in M13K07 genome. It was identified as the RBS for gene III message by Wezenbeek et al in Gene (1980) 11: 129-148 New England Biolabs M13K07. Sequenced in 2002 by L. Panganaban
mutable notes: none
mutable provenance: New England Biolabs M13K07. Sequenced in 2002 by L. Panganaban
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_M13503_sequence/1

BBa_I742156
type: ComponentDefinition
persistent identity: BBa I742156
display id: BBa_I742156
version: 1
was generated by: iGEM to SBOL conversion
description: crtZ native rbs
creator: Chris French
created: 2007-10-23T11:00:00Z
modified: 2015-08-31T04:08:03Z
discontinued: false
dominant: false
experience: experience/None
group u list: _123_
m user id: 0
owner id: 837
owning group id: 163
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Native ribosome binding site for crtZ (beta-carotene hydroxylase) from Pantoea ananatis (formerly Erwinia uredovora) DSMZ 30080 (ATCC 19321). Accession: D90087.
mutable notes: A virtual part to be added to the coding sequence blunt (without a scar).
mutable provenance: Pantoea ananatis (formerly Erwinia uredovora) DSMZ 30080 (ATCC 19321). Accession: D90087.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_I742156_sequence/1

BBa_J61126
type: ComponentDefinition
persistent identity: BBa J61126
display id: BBa_J61126
version: 1
was generated by: iGEM to SBOL conversion
description: Ribosome Binding Site Family Member
creator: John Anderson
created: 2007-04-24T11:00:00Z
modified: 2015-08-31T01:59:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable description: {{JCA_Arkin_RBSFamily}}
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J61126_sequence/1

BBa_B0072
type: ComponentDefinition
persistent identity: BBa B0072
display id: BBa_B0072
version: 1
was generated by: iGEM to SBOL conversion
description: orthogonal RBS
creator: Bartholomew Canton
created: 2006-05-01T11:00:00Z
modified: 2015-08-31T04:07:21Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_6_
m user id: 0
owner id: 135
owning group id: 6
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_B0072_sequence/1

BBa_K1675000
type: ComponentDefinition
persistent identity: BBa K1675000
display id: BBa_K1675000
version: 1
was generated by: iGEM to SBOL conversion
description: NhaA; sodion-proton antiporter; membrane protein
created: 2015-09-08T11:00:00Z
modified: 2015-09-18T11:49:20Z
mutable provenance: Escherichia coli str. K-12 substr. Sequence found through Genbank http://www.ncbi.nlm.nih.gov/nuccore/NC_000913.3?report=genbank&from=17489&to=18655
owned by: james
owned by: myers
mutable description: The gene,NhaA, is the archetypal sodium-proton antiporter in E. coli. It is the only member of the family that is absolutely required by E. coli for survival in high-salt conditions, under alkaline stress, or in the presence of otherwise toxic Li+ concentration. NhaA catalyzes the exchange of 2 H+ per Na+. Therefore, nhaA could make sure E. coli???s survival under alkaline pH, which are in desperate need in our project.
discontinued: false
dominant: false
experience: experience/None
group u list: _2093_
m user id: 27740
owner id: 27740
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: None.
star: false
creator: JIAQI XU
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: GTG Start Codon
sequence annotation: TGA Stop Codon
sequence annotation: NhaA Coding Region
sequence: https://synbiohub.org/public/igem/BBa_K1675000_sequence/1

GTG Start Codon
type: SequenceAnnotation
persistent identity: annotation2447756
display id: annotation2447756
version: 1
top level: BBa_K1675000
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1675000/annotation2447756/range2447756/1

https://synbiohub.org/public/igem/BBa_K1675000/annotation2447756/range2447756/1
type: Range
persistent identity: range2447756
display id: range2447756
version: 1
top level: BBa_K1675000
direction: direction/forward
start: 1
end: 3
orientation: inline

TGA Stop Codon
type: SequenceAnnotation
persistent identity: annotation2447758
display id: annotation2447758
version: 1
top level: BBa_K1675000
role: feature/stop
role: SO:0000319
location: https://synbiohub.org/public/igem/BBa_K1675000/annotation2447758/range2447758/1

https://synbiohub.org/public/igem/BBa_K1675000/annotation2447758/range2447758/1
type: Range
persistent identity: range2447758
display id: range2447758
version: 1
top level: BBa_K1675000
direction: direction/forward
start: 1165
end: 1167
orientation: inline

NhaA Coding Region
type: SequenceAnnotation
persistent identity: annotation2447757
display id: annotation2447757
version: 1
top level: BBa_K1675000
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1675000/annotation2447757/range2447757/1

https://synbiohub.org/public/igem/BBa_K1675000/annotation2447757/range2447757/1
type: Range
persistent identity: range2447757
display id: range2447757
version: 1
top level: BBa_K1675000
direction: direction/forward
start: 1
end: 1167
orientation: inline

BBa_J61110
type: ComponentDefinition
persistent identity: BBa J61110
display id: BBa_J61110
version: 1
was generated by: iGEM to SBOL conversion
description: Ribosome Binding Site Family Member
creator: John Anderson
created: 2007-04-23T11:00:00Z
modified: 2015-08-31T02:03:00Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable description: {{JCA_Arkin_RBSFamily}}
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J61110_sequence/1

BBa_B2101
type: ComponentDefinition
persistent identity: BBa B2101
display id: BBa_B2101
version: 1
was generated by: iGEM to SBOL conversion
description: T7 RBS 0.3 + SapI (rev)
creator: Heather Keller
created: 2006-09-27T11:00:00Z
modified: 2015-08-31T04:07:21Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_
m user id: 0
owner id: 571
owning group id: 10
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This version of the T7 0.3 RBS is designed for construction via Heather Keller's alternative assembly plan using XbaI and SapI for RBS/CDS assembly without a mixed site. Contains the -20 to -1 region of gp0.4 from the wild-type T7 genome. Can also be used with Standard BioBricks assembly, although this will add a 7 amino acid fusion to the N-terminus of the resulting protein.
mutable notes: This RBS is designed to be compatible with Heather Keller's RBS assembly scheme for creating RBS/CDS junctions without a mixed site. For use in this scheme, this part should be cut with XbaI and SapI. It must be used with a coding sequence containing the SapI site at the 5' end. This part is not compatible with CDSs that use a GTG site and must be used with CDSs using an ATG start site. The two bp spacer on the 3' end also allows this part to be used in standard biobricks assembly and is necessary to maintain the reading frame of a downstream CDS. Note that Standard Biobricks Assembly with this part results in the creation of a new ATG start site, and a 7 amino acid fusion at the N terminus of the downstream protein. CDSs with alternative start sites are compatible with this part via Standard Assembly. This part was inadvertently constructed without the single base pair (G/C) that separates the XbaI site and the 5' end of the part.
mutable provenance: The RBS is taken from the wild-type bacteriophage T7 genome. Please see part B2001 for more information.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/DNA
role: SO:0000110
component: BBa_B2001
sequence annotation: BBa_B2001
sequence: https://synbiohub.org/public/igem/BBa_B2101_sequence/1

BBa_B2001
type: Component
persistent identity: component2218355
display id: component2218355
version: 1
top level: BBa_B2101
access: public
definition: T7 0.3

BBa_B2001
type: SequenceAnnotation
persistent identity: annotation2218355
display id: annotation2218355
version: 1
top level: BBa_B2101
location: https://synbiohub.org/public/igem/BBa_B2101/annotation2218355/range2218355/1
component: BBa_B2001

https://synbiohub.org/public/igem/BBa_B2101/annotation2218355/range2218355/1
type: Range
persistent identity: range2218355
display id: range2218355
version: 1
direction: direction/reverse
top level: BBa_B2101
start: 1
end: 20
orientation: inline

BBa_J61100
type: ComponentDefinition
persistent identity: BBa J61100
display id: BBa_J61100
version: 1
was generated by: iGEM to SBOL conversion
description: Ribosome Binding Site Family Member
creator: John Anderson
created: 2007-01-28T12:00:00Z
modified: 2015-08-31T02:03:00Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable description: {{JCA_Arkin_RBSFamily}}
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J61100_sequence/1

T7 0.4
type: ComponentDefinition
persistent identity: BBa B2002
display id: BBa_B2002
version: 1
was generated by: iGEM to SBOL conversion
description: T7 RBS 0.4
creator: Heather Keller
created: 2006-09-27T11:00:00Z
modified: 2015-08-31T04:07:21Z
discontinued: false
dominant: true
experience: experience/None
group u list: _11_
m user id: 0
owner id: 571
owning group id: 10
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is the -20 to -1 region of T7 gp0.7, which contains the native RBS region for this gene. The physical instantiation of this part does not exist. This part is not compatible with Standard Biobricks assembly methods, as the scar created during assembly would interrupt the spacing between the RBS and the start codon of the CDS. Individuals wishing to use this RBS sequence in their assembles should refer to part XXXXX. (Information forthcoming)
mutable notes: The -20 to -1 region was chosen to be representative of the RBS region of the T7 genes for the following reasons: 1) The Shine-Delgarno region is contained within this defined region for all T7 genes. 2) Current literature suggests that when bound to the Translation Start Site (0), the Ribosome covers positions -20 to +13.
mutable provenance: Genome of wild-type bacteriophage T7, positions 2001-2020. Includes the -20 to -1 region of gp0.7.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: SD region
sequence: https://synbiohub.org/public/igem/BBa_B2002_sequence/1

SD region
type: SequenceAnnotation
persistent identity: annotation1902419
display id: annotation1902419
version: 1
top level: T7 0.4
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_B2002/annotation1902419/range1902419/1

https://synbiohub.org/public/igem/BBa_B2002/annotation1902419/range1902419/1
type: Range
persistent identity: range1902419
display id: range1902419
version: 1
direction: direction/forward
top level: T7 0.4
start: 4
end: 10
orientation: inline

BBa_B0032
type: ComponentDefinition
persistent identity: BBa B0032
display id: BBa_B0032
version: 1
was generated by: iGEM to SBOL conversion
description: RBS.3 (medium) -- derivative of BBa_0030
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Weak1 RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0033</bb_part>.
discontinued: false
dominant: true
experience: experience/Works
group u list: _41_44_48_46_1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("RBS-2" in figure 4-14 of thesis). Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: RBS
sequence annotation: RBS-3\Weak
sequence annotation: BBa_B0032
sequence: https://synbiohub.org/public/igem/BBa_B0032_sequence/1

RBS
type: SequenceAnnotation
persistent identity: annotation1710
display id: annotation1710
version: 1
top level: BBa_B0032
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_B0032/annotation1710/range1710/1

https://synbiohub.org/public/igem/BBa_B0032/annotation1710/range1710/1
type: Range
persistent identity: range1710
display id: range1710
version: 1
top level: BBa_B0032
direction: direction/forward
start: 7
end: 10
orientation: inline

RBS-3\Weak
type: SequenceAnnotation
persistent identity: annotation1709
display id: annotation1709
version: 1
top level: BBa_B0032
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_B0032/annotation1709/range1709/1

https://synbiohub.org/public/igem/BBa_B0032/annotation1709/range1709/1
type: Range
persistent identity: range1709
display id: range1709
version: 1
top level: BBa_B0032
direction: direction/forward
start: 1
end: 13
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation7027
display id: annotation7027
version: 1
top level: BBa_B0032
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_B0032/annotation7027/range7027/1

https://synbiohub.org/public/igem/BBa_B0032/annotation7027/range7027/1
type: Range
persistent identity: range7027
display id: range7027
version: 1
top level: BBa_B0032
direction: direction/forward
start: 1
end: 13
orientation: inline

BBa_B0070
type: ComponentDefinition
persistent identity: BBa B0070
display id: BBa_B0070
version: 1
was generated by: iGEM to SBOL conversion
description: Specialized RBS modified from that of B0036 (Brink et al.)
creator: Barry Canton
created: 2006-03-03T12:00:00Z
modified: 2015-08-31T04:07:21Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_6_
m user id: 0
owner id: 135
owning group id: 6
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This RBS has the modified Shine-Dalgarno sequence, it has a reduced secondary structure relative to using the actual Brink sequence with mixed connective sites flanking it. the spacing between the SD and the ATG is the same as that used by Brink et al. even though it is non-optimal for E. coli
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_B0070_sequence/1

BBa_B0015
type: ComponentDefinition
persistent identity: BBa B0015
display id: BBa_B0015
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0010-B0012)
created: 2003-07-16T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator consisting of BBa_B0010 and BBa_B0012
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_B0015_sequence/1

BBa_B0012
type: Component
persistent identity: component1916612
display id: component1916612
version: 1
top level: BBa_B0015
access: public
definition: BBa_B0012

BBa_B0010
type: Component
persistent identity: component1916610
display id: component1916610
version: 1
top level: BBa_B0015
access: public
definition: BBa_B0010

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1916610
display id: annotation1916610
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
type: Range
persistent identity: range1916610
display id: range1916610
version: 1
top level: BBa_B0015
direction: direction/forward
start: 1
end: 80
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1916612
display id: annotation1916612
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
type: Range
persistent identity: range1916612
display id: range1916612
version: 1
top level: BBa_B0015
direction: direction/forward
start: 89
end: 129
orientation: inline

BBa_B0033
type: ComponentDefinition
persistent identity: BBa B0033
display id: BBa_B0033
version: 1
was generated by: iGEM to SBOL conversion
description: RBS.4 (weaker) -- derivative of BBa_0030
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _41_44_48_46_1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Weaker RBS based on Ron Weiss thesis. Strengths relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>.
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("RBS-3" in figure 4-14 of thesis). No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: RBS-4\Weaker
sequence annotation: BBa_B0033
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_B0033_sequence/1

RBS-4\Weaker
type: SequenceAnnotation
persistent identity: annotation1713
display id: annotation1713
version: 1
top level: BBa_B0033
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_B0033/annotation1713/range1713/1

https://synbiohub.org/public/igem/BBa_B0033/annotation1713/range1713/1
type: Range
persistent identity: range1713
display id: range1713
version: 1
direction: direction/forward
top level: BBa_B0033
start: 1
end: 11
orientation: inline

BBa_B0033
type: SequenceAnnotation
persistent identity: annotation7028
display id: annotation7028
version: 1
top level: BBa_B0033
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_B0033/annotation7028/range7028/1

https://synbiohub.org/public/igem/BBa_B0033/annotation7028/range7028/1
type: Range
persistent identity: range7028
display id: range7028
version: 1
direction: direction/forward
top level: BBa_B0033
start: 1
end: 11
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1714
display id: annotation1714
version: 1
top level: BBa_B0033
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_B0033/annotation1714/range1714/1

https://synbiohub.org/public/igem/BBa_B0033/annotation1714/range1714/1
type: Range
persistent identity: range1714
display id: range1714
version: 1
direction: direction/forward
top level: BBa_B0033
start: 7
end: 10
orientation: inline

BBa_J34803
type: ComponentDefinition
persistent identity: BBa J34803
display id: BBa_J34803
version: 1
was generated by: iGEM to SBOL conversion
description: ribosome binding site
creator: Alexandra Choutko
created: 2006-10-25T11:00:00Z
modified: 2015-08-31T04:08:46Z
discontinued: false
dominant: false
experience: experience/None
group u list: _62_
m user id: 0
owner id: 1085
owning group id: 62
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: a
mutable notes: a
mutable provenance: a
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J34803_sequence/1

BBa_J64030
type: ComponentDefinition
persistent identity: BBa J64030
display id: BBa_J64030
version: 1
was generated by: iGEM to SBOL conversion
description: sopD RBS
creator: Dan Widmaier
created: 2008-01-16T12:00:00Z
modified: 2015-08-31T01:56:26Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _98_
m user id: 0
owner id: 2407
owning group id: 98
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is the natural RBS from the sopD gene in Salmonella Type III Secretion.
mutable notes: This part was designed without consideration for biobrick standard assembly. This RBS has not been explicitly tested for expression rate.
mutable provenance: Salmonella LT2 Genomic DNA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: sopD RBS
sequence: https://synbiohub.org/public/igem/BBa_J64030_sequence/1

sopD RBS
type: SequenceAnnotation
persistent identity: annotation1959634
display id: annotation1959634
version: 1
top level: BBa_J64030
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_J64030/annotation1959634/range1959634/1

https://synbiohub.org/public/igem/BBa_J64030/annotation1959634/range1959634/1
type: Range
persistent identity: range1959634
display id: range1959634
version: 1
direction: direction/forward
top level: BBa_J64030
start: 1
end: 17
orientation: inline

creB RBS
type: ComponentDefinition
persistent identity: BBa J64968
display id: BBa_J64968
version: 1
was generated by: iGEM to SBOL conversion
description: RBS on creB
creator: Kristopher Kuchenbecker
created: 2007-03-19T12:00:00Z
modified: 2015-05-08T01:08:17Z
discontinued: false
dominant: false
experience: experience/None
group u list: _98_
m user id: 0
owner id: 1429
owning group id: 98
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Ribosome Binding Site
mutable notes: RBS
mutable provenance: ''E. coli'' K-12 genome
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J64968_sequence/1

BBa_B0029
type: ComponentDefinition
persistent identity: BBa B0029
display id: BBa_B0029
version: 1
was generated by: iGEM to SBOL conversion
description: 1 bp deletion from B0031
creator: Jason Kelly
created: 2008-02-07T12:00:00Z
modified: 2015-08-31T04:07:20Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_
m user id: 0
owner id: 2
owning group id: 84
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: .
mutable notes: .
mutable provenance: .
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_B0029_sequence/1

BBa_J61132
type: ComponentDefinition
persistent identity: BBa J61132
display id: BBa_J61132
version: 1
was generated by: iGEM to SBOL conversion
description: Ribosome Binding Site Family Member
creator: John Anderson
created: 2007-04-24T11:00:00Z
modified: 2015-08-31T01:59:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable description: {{JCA_Arkin_RBSFamily}}
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J61132_sequence/1

BBa_J61103
type: ComponentDefinition
persistent identity: BBa J61103
display id: BBa_J61103
version: 1
was generated by: iGEM to SBOL conversion
description: Ribosome Binding Site Family Member
creator: John Anderson
created: 2007-04-23T11:00:00Z
modified: 2015-08-31T02:03:00Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable description: {{JCA_Arkin_RBSFamily}}
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J61103_sequence/1

BBa_J34810
type: ComponentDefinition
persistent identity: BBa J34810
display id: BBa_J34810
version: 1
was generated by: iGEM to SBOL conversion
description: ribosome binding site
creator: Alexandra Choutko
created: 2006-10-25T11:00:00Z
modified: 2015-08-31T04:08:47Z
discontinued: false
dominant: false
experience: experience/None
group u list: _62_
m user id: 0
owner id: 1085
owning group id: 62
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: a
mutable notes: a
mutable provenance: a
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J34810_sequence/1

BBa_I723014
type: ComponentDefinition
persistent identity: BBa I723014
display id: BBa_I723014
version: 1
was generated by: iGEM to SBOL conversion
description: Estimated RBS for DntA
creator: Scott Ramsay
created: 2007-10-24T11:00:00Z
modified: 2015-08-31T04:07:53Z
discontinued: false
dominant: false
experience: experience/None
group u list: _126_
m user id: 0
owner id: 2140
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is the naturally-occurring RBS found in the regulatory region of the DntA gene.
mutable notes: '''''This does not physically exist as a basic part due to time constraints; it was cloned as part of composite part which incorporates all of the components necessary for expression of a reporter gene under the control of DntR'''''. For this reason, the ends of the supplied sequence may not accurately represent the boundaries of the functional unit.
mutable provenance: Cloned from a historical plasmid based on pQF52 carrying genomic sequences from ''Burkholderia cepacia''.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: misc
sequence: https://synbiohub.org/public/igem/BBa_I723014_sequence/1

misc
type: SequenceAnnotation
persistent identity: annotation1954961
display id: annotation1954961
version: 1
top level: BBa_I723014
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I723014/annotation1954961/range1954961/1

https://synbiohub.org/public/igem/BBa_I723014/annotation1954961/range1954961/1
type: Range
persistent identity: range1954961
display id: range1954961
version: 1
direction: direction/forward
top level: BBa_I723014
start: 1
end: 25
orientation: inline

BBa_J61134
type: ComponentDefinition
persistent identity: BBa J61134
display id: BBa_J61134
version: 1
was generated by: iGEM to SBOL conversion
description: Ribosome Binding Site Family Member
creator: John Anderson
created: 2007-04-24T11:00:00Z
modified: 2015-08-31T01:59:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable description: {{JCA_Arkin_RBSFamily}}
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J61134_sequence/1

BBa_I742153
type: ComponentDefinition
persistent identity: BBa I742153
display id: BBa_I742153
version: 1
was generated by: iGEM to SBOL conversion
description: crtY native rbs
creator: Chris French
created: 2007-10-23T11:00:00Z
modified: 2015-08-31T04:08:03Z
discontinued: false
dominant: false
experience: experience/None
group u list: _123_
m user id: 0
owner id: 837
owning group id: 163
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Native ribosome binding site of crtY (lycopene cyclase) from Pantoea ananatis (formerly Erwinia uredovora) DSMZ 30080 (ATCC 19321). Accession: D90087.
mutable notes: This is a virtual part to be added, blunt-ended, to the coding sequence.
mutable provenance: Pantoea ananatis (formerly Erwinia uredovora) DSMZ 30080 (ATCC 19321). Accession: D90087.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_I742153_sequence/1

BBa_K150005
type: ComponentDefinition
persistent identity: BBa K150005
display id: BBa_K150005
version: 1
was generated by: iGEM to SBOL conversion
description: ribosome binding site of pTrc99a
creator: Pascal Kraemer
created: 2008-10-20T11:00:00Z
modified: 2015-05-08T01:10:46Z
discontinued: false
dominant: false
experience: experience/None
group u list: _234_
m user id: 0
owner id: 3299
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: ribosome binding site of pTrc99a
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: BBa_K150005
sequence: https://synbiohub.org/public/igem/BBa_K150005_sequence/1

BBa_K150005
type: SequenceAnnotation
persistent identity: annotation1983190
display id: annotation1983190
version: 1
top level: BBa_K150005
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K150005/annotation1983190/range1983190/1

https://synbiohub.org/public/igem/BBa_K150005/annotation1983190/range1983190/1
type: Range
persistent identity: range1983190
display id: range1983190
version: 1
direction: direction/forward
top level: BBa_K150005
start: 1
end: 13
orientation: inline

BBa_J61119
type: ComponentDefinition
persistent identity: BBa J61119
display id: BBa_J61119
version: 1
was generated by: iGEM to SBOL conversion
description: Ribosome Binding Site Family Member
creator: John Anderson
created: 2007-04-24T11:00:00Z
modified: 2015-08-31T01:59:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable description: {{JCA_Arkin_RBSFamily}}
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J61119_sequence/1

BBa_K1390019
type: ComponentDefinition
persistent identity: BBa K1390019
display id: BBa_K1390019
version: 1
was generated by: iGEM to SBOL conversion
description: Complete sMMO of Methylococcus capsulatus
creator: Torsten Meyer
created: 2014-09-03T11:00:00Z
modified: 2015-05-08T01:10:14Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1767_
m user id: 0
owner id: 17096
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: The part consists of the six subunits of methane monooxygenase (MMO). Gene order: mmoX, mmoY, mmoB, mmoZ, mmoD, mmoC The expression is under control of lac+pl. MMO converts methane to methanol.
mutable notes: Part is designed considering the RFC10 standard.
mutable provenance: Methylococcus capsulatus strain Bath
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K1390001
component: BBa_K1390003
component: BBa_B0032
component: BBa_K1390005
component: BBa_R0011
component: BBa_K1390002
component: BBa_K1390006
component: BBa_B0015
component: BBa_K1390004
sequence annotation: BBa_B0032
sequence annotation: BBa_K1390005
sequence annotation: BBa_K1390006
sequence annotation: BBa_K1390004
sequence annotation: BBa_K1390003
sequence annotation: BBa_B0015
sequence annotation: BBa_K1390001
sequence annotation: BBa_K1390002
sequence annotation: BBa_R0011
sequence: https://synbiohub.org/public/igem/BBa_K1390019_sequence/1

BBa_K1390001
type: Component
persistent identity: component2412365
display id: component2412365
version: 1
top level: BBa_K1390019
access: public
definition: BBa_K1390001

BBa_K1390003
type: Component
persistent identity: component2412373
display id: component2412373
version: 1
top level: BBa_K1390019
access: public
definition: BBa_K1390003

BBa_B0032
type: Component
persistent identity: component2412367
display id: component2412367
version: 1
top level: BBa_K1390019
access: public
definition: BBa_B0032

BBa_B0032
type: Component
persistent identity: component2412359
display id: component2412359
version: 1
top level: BBa_K1390019
access: public
definition: BBa_B0032

BBa_B0032
type: Component
persistent identity: component2412371
display id: component2412371
version: 1
top level: BBa_K1390019
access: public
definition: BBa_B0032

BBa_K1390005
type: Component
persistent identity: component2412361
display id: component2412361
version: 1
top level: BBa_K1390019
access: public
definition: BBa_K1390005

BBa_B0032
type: Component
persistent identity: component2412355
display id: component2412355
version: 1
top level: BBa_K1390019
access: public
definition: BBa_B0032

BBa_B0032
type: Component
persistent identity: component2412363
display id: component2412363
version: 1
top level: BBa_K1390019
access: public
definition: BBa_B0032

BBa_B0032
type: Component
persistent identity: component2412375
display id: component2412375
version: 1
top level: BBa_K1390019
access: public
definition: BBa_B0032

BBa_R0011
type: Component
persistent identity: component2412349
display id: component2412349
version: 1
top level: BBa_K1390019
access: public
definition: lacI+pL

BBa_K1390002
type: Component
persistent identity: component2412377
display id: component2412377
version: 1
top level: BBa_K1390019
access: public
definition: BBa_K1390002

BBa_K1390006
type: Component
persistent identity: component2412369
display id: component2412369
version: 1
top level: BBa_K1390019
access: public
definition: BBa_K1390006

BBa_B0015
type: Component
persistent identity: component2412384
display id: component2412384
version: 1
top level: BBa_K1390019
access: public
definition: BBa_B0015

BBa_K1390004
type: Component
persistent identity: component2412357
display id: component2412357
version: 1
top level: BBa_K1390019
access: public
definition: BBa_K1390004

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation2412371
display id: annotation2412371
version: 1
top level: BBa_K1390019
location: https://synbiohub.org/public/igem/BBa_K1390019/annotation2412371/range2412371/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_K1390019/annotation2412371/range2412371/1
type: Range
persistent identity: range2412371
display id: range2412371
version: 1
direction: direction/forward
top level: BBa_K1390019
start: 3862
end: 3874
orientation: inline

BBa_K1390005
type: SequenceAnnotation
persistent identity: annotation2412361
display id: annotation2412361
version: 1
top level: BBa_K1390019
location: https://synbiohub.org/public/igem/BBa_K1390019/annotation2412361/range2412361/1
component: BBa_K1390005

https://synbiohub.org/public/igem/BBa_K1390019/annotation2412361/range2412361/1
type: Range
persistent identity: range2412361
display id: range2412361
version: 1
direction: direction/reverse
top level: BBa_K1390019
start: 1694
end: 2863
orientation: inline

BBa_K1390006
type: SequenceAnnotation
persistent identity: annotation2412369
display id: annotation2412369
version: 1
top level: BBa_K1390019
location: https://synbiohub.org/public/igem/BBa_K1390019/annotation2412369/range2412369/1
component: BBa_K1390006

https://synbiohub.org/public/igem/BBa_K1390019/annotation2412369/range2412369/1
type: Range
persistent identity: range2412369
display id: range2412369
version: 1
direction: direction/reverse
top level: BBa_K1390019
start: 3344
end: 3853
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation2412367
display id: annotation2412367
version: 1
top level: BBa_K1390019
location: https://synbiohub.org/public/igem/BBa_K1390019/annotation2412367/range2412367/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_K1390019/annotation2412367/range2412367/1
type: Range
persistent identity: range2412367
display id: range2412367
version: 1
direction: direction/forward
top level: BBa_K1390019
start: 3325
end: 3337
orientation: inline

BBa_K1390004
type: SequenceAnnotation
persistent identity: annotation2412357
display id: annotation2412357
version: 1
top level: BBa_K1390019
location: https://synbiohub.org/public/igem/BBa_K1390019/annotation2412357/range2412357/1
component: BBa_K1390004

https://synbiohub.org/public/igem/BBa_K1390019/annotation2412357/range2412357/1
type: Range
persistent identity: range2412357
display id: range2412357
version: 1
direction: direction/reverse
top level: BBa_K1390019
start: 83
end: 1666
orientation: inline

BBa_K1390003
type: SequenceAnnotation
persistent identity: annotation2412373
display id: annotation2412373
version: 1
top level: BBa_K1390019
location: https://synbiohub.org/public/igem/BBa_K1390019/annotation2412373/range2412373/1
component: BBa_K1390003

https://synbiohub.org/public/igem/BBa_K1390019/annotation2412373/range2412373/1
type: Range
persistent identity: range2412373
display id: range2412373
version: 1
direction: direction/reverse
top level: BBa_K1390019
start: 3881
end: 4192
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2412384
display id: annotation2412384
version: 1
top level: BBa_K1390019
location: https://synbiohub.org/public/igem/BBa_K1390019/annotation2412384/range2412384/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K1390019/annotation2412384/range2412384/1
type: Range
persistent identity: range2412384
display id: range2412384
version: 1
direction: direction/reverse
top level: BBa_K1390019
start: 5275
end: 5403
orientation: inline

BBa_K1390001
type: SequenceAnnotation
persistent identity: annotation2412365
display id: annotation2412365
version: 1
top level: BBa_K1390019
location: https://synbiohub.org/public/igem/BBa_K1390019/annotation2412365/range2412365/1
component: BBa_K1390001

https://synbiohub.org/public/igem/BBa_K1390019/annotation2412365/range2412365/1
type: Range
persistent identity: range2412365
display id: range2412365
version: 1
direction: direction/reverse
top level: BBa_K1390019
start: 2891
end: 3316
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation2412375
display id: annotation2412375
version: 1
top level: BBa_K1390019
location: https://synbiohub.org/public/igem/BBa_K1390019/annotation2412375/range2412375/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_K1390019/annotation2412375/range2412375/1
type: Range
persistent identity: range2412375
display id: range2412375
version: 1
direction: direction/forward
top level: BBa_K1390019
start: 4201
end: 4213
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation2412355
display id: annotation2412355
version: 1
top level: BBa_K1390019
location: https://synbiohub.org/public/igem/BBa_K1390019/annotation2412355/range2412355/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_K1390019/annotation2412355/range2412355/1
type: Range
persistent identity: range2412355
display id: range2412355
version: 1
direction: direction/forward
top level: BBa_K1390019
start: 64
end: 76
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation2412363
display id: annotation2412363
version: 1
top level: BBa_K1390019
location: https://synbiohub.org/public/igem/BBa_K1390019/annotation2412363/range2412363/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_K1390019/annotation2412363/range2412363/1
type: Range
persistent identity: range2412363
display id: range2412363
version: 1
direction: direction/forward
top level: BBa_K1390019
start: 2872
end: 2884
orientation: inline

BBa_K1390002
type: SequenceAnnotation
persistent identity: annotation2412377
display id: annotation2412377
version: 1
top level: BBa_K1390019
location: https://synbiohub.org/public/igem/BBa_K1390019/annotation2412377/range2412377/1
component: BBa_K1390002

https://synbiohub.org/public/igem/BBa_K1390019/annotation2412377/range2412377/1
type: Range
persistent identity: range2412377
display id: range2412377
version: 1
direction: direction/reverse
top level: BBa_K1390019
start: 4220
end: 5266
orientation: inline

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation2412349
display id: annotation2412349
version: 1
top level: BBa_K1390019
location: https://synbiohub.org/public/igem/BBa_K1390019/annotation2412349/range2412349/1
component: BBa_R0011

https://synbiohub.org/public/igem/BBa_K1390019/annotation2412349/range2412349/1
type: Range
persistent identity: range2412349
display id: range2412349
version: 1
direction: direction/forward
top level: BBa_K1390019
start: 1
end: 54
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation2412359
display id: annotation2412359
version: 1
top level: BBa_K1390019
location: https://synbiohub.org/public/igem/BBa_K1390019/annotation2412359/range2412359/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_K1390019/annotation2412359/range2412359/1
type: Range
persistent identity: range2412359
display id: range2412359
version: 1
direction: direction/forward
top level: BBa_K1390019
start: 1675
end: 1687
orientation: inline

BBa_Z0261
type: ComponentDefinition
persistent identity: BBa Z0261
display id: BBa_Z0261
version: 1
was generated by: iGEM to SBOL conversion
description: Strong T7.2 RBS
creator: Sriram Kosuri
created: 2006-05-15T11:00:00Z
modified: 2015-05-08T01:14:55Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_10_
m user id: 0
owner id: 64
owning group id: 10
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Sequence comes from the natural gene 5 RBS. Using gene 5 as a strong RBS is from initial results from experiments characterizing all natural T7 RBSs.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_Z0261_sequence/1

BBa_J64807
type: ComponentDefinition
persistent identity: BBa J64807
display id: BBa_J64807
version: 1
was generated by: iGEM to SBOL conversion
description: RocD RBS
creator: David Stanley
created: 2007-03-19T12:00:00Z
modified: 2015-05-08T01:08:17Z
discontinued: false
dominant: false
experience: experience/None
group u list: _98_
m user id: 0
owner id: 1423
owning group id: 98
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: RocD RBS
mutable notes: none
mutable provenance: none
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J64807_sequence/1

BBa_J61143
type: ComponentDefinition
persistent identity: BBa J61143
display id: BBa_J61143
version: 1
was generated by: iGEM to SBOL conversion
description: {rbs1} Library Member in BBb
creator: John Anderson
created: 2007-09-03T11:00:00Z
modified: 2015-08-31T01:56:25Z
discontinued: false
dominant: false
experience: experience/None
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: {{BBbRBS1Family}}
mutable notes: {{BerkiGEM2007-Biobrick2.0Intro}}
mutable provenance: Synthetic
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J61143_sequence/1

BBa_I742159
type: ComponentDefinition
persistent identity: BBa I742159
display id: BBa_I742159
version: 1
was generated by: iGEM to SBOL conversion
description: crtI native rbs
creator: Chris French
created: 2007-10-23T11:00:00Z
modified: 2015-08-31T04:08:03Z
discontinued: false
dominant: false
experience: experience/None
group u list: _123_
m user id: 0
owner id: 837
owning group id: 163
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Native rbs from crtI (phytoene dehydrogenase) of Pantoea ananatis (formerly Erwinia uredovora) DSMZ 30080 (ATCC 19321). Accession: D90087.
mutable notes: Virtual part to be added to the coding sequence.
mutable provenance: Pantoea ananatis (formerly Erwinia uredovora) DSMZ 30080 (ATCC 19321). Accession: D90087.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: rbs
sequence: https://synbiohub.org/public/igem/BBa_I742159_sequence/1

rbs
type: SequenceAnnotation
persistent identity: annotation1953816
display id: annotation1953816
version: 1
top level: BBa_I742159
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_I742159/annotation1953816/range1953816/1

https://synbiohub.org/public/igem/BBa_I742159/annotation1953816/range1953816/1
type: Range
persistent identity: range1953816
display id: range1953816
version: 1
direction: direction/forward
top level: BBa_I742159
start: 10
end: 15
orientation: inline

BBa_J61111
type: ComponentDefinition
persistent identity: BBa J61111
display id: BBa_J61111
version: 1
was generated by: iGEM to SBOL conversion
description: Ribosome Binding Site Family Member
creator: John Anderson
created: 2007-04-23T11:00:00Z
modified: 2015-08-31T02:03:00Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable description: {{JCA_Arkin_RBSFamily}}
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J61111_sequence/1

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: RBS based on Elowitz repressilator.
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
direction: direction/forward
top level: BBa_B0034
start: 5
end: 8
orientation: inline

BBa_K1390005
type: ComponentDefinition
persistent identity: BBa K1390005
display id: BBa_K1390005
version: 1
was generated by: iGEM to SBOL conversion
description: mmoY
created: 2014-07-02T11:00:00Z
modified: 2015-05-08T01:10:14Z
mutable provenance: Amplified from the genomic DNA of Methylococcus capsulatus bath without any PstI restriction site
owned by: james
owned by: myers
mutable description: mmoY is one subunit of the mmo from Methylococcus capsulatus bath
discontinued: false
dominant: false
group u list: _1767_
m user id: 0
owner id: 17096
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: the brick is constructed in the RCF10 standard
star: false
creator: Stefan D??bel
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence: https://synbiohub.org/public/igem/BBa_K1390005_sequence/1

BBa_K1390001
type: ComponentDefinition
persistent identity: BBa K1390001
display id: BBa_K1390001
version: 1
was generated by: iGEM to SBOL conversion
description: mmoB
created: 2014-07-02T11:00:00Z
modified: 2015-05-08T01:10:13Z
mutable provenance: Amplified from the genomic DNA of Methylococcus capsulatus bath
owned by: james
owned by: myers
mutable description: mmoB is one subunit of the mmo from Methylococcus capsulatus bath
discontinued: false
dominant: false
group u list: _1767_
m user id: 0
owner id: 17096
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: the brick is constructed in the RCF10 standard
star: false
creator: Stefan D??bel
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence: https://synbiohub.org/public/igem/BBa_K1390001_sequence/1

BBa_J23080
type: ComponentDefinition
persistent identity: BBa J23080
display id: BBa_J23080
version: 1
was generated by: iGEM to SBOL conversion
description: b0034 derived RBS to match spacing of J01122
creator: bryan hernandez
created: 2006-09-20T11:00:00Z
modified: 2015-08-31T04:08:40Z
discontinued: false
dominant: false
experience: experience/None
group u list: _52_
m user id: 0
owner id: 936
owning group id: 52
sample status: In stock
status: status/Available
bookmark: false
mutable description: RBS spacing modification to [RBS] such that the spacing between the shine-delgarno and the ORF is the same as it is in J01122.
mutable notes: none
mutable provenance: j01122
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J23080_sequence/1

BBa_M13511
type: ComponentDefinition
persistent identity: BBa M13511
display id: BBa_M13511
version: 1
was generated by: iGEM to SBOL conversion
description: M13K07 gene XI RBS
creator: Natalie Kuldell
created: 2006-12-14T12:00:00Z
modified: 2015-05-08T01:13:56Z
discontinued: false
dominant: false
experience: experience/None
group u list: _45_
m user id: 0
owner id: 314
owning group id: 1
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This RBS is part of the bacteriophage M13 genome, initiating translation of the gene XI message (BBa_M13011). The part aligns with base pairs 3900-3915 in M13K07 genome. It was NOT identified as the RBS for gene XI message by Wezenbeek et al in Gene (1980) 11: 129-148
mutable notes: none
mutable provenance: New England Biolabs M13K07. Sequenced in 2002 by L. Panganaban
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_M13511_sequence/1

BBa_J64018
type: ComponentDefinition
persistent identity: BBa J64018
display id: BBa_J64018
version: 1
was generated by: iGEM to SBOL conversion
description: invJ RBS
creator: Dan Widmaier
created: 2008-01-16T12:00:00Z
modified: 2015-08-31T01:56:26Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _98_
m user id: 0
owner id: 2407
owning group id: 98
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This part is the natural RBS associated with the invJ protein in Salmonella Typhimurium LT2.
mutable notes: This part was not designed with compliance to biobrick standard assembly in mind.
mutable provenance: Salmonella Typhimurium LT2 Genomic DNA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: invJ RBS
sequence: https://synbiohub.org/public/igem/BBa_J64018_sequence/1

invJ RBS
type: SequenceAnnotation
persistent identity: annotation1959617
display id: annotation1959617
version: 1
top level: BBa_J64018
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_J64018/annotation1959617/range1959617/1

https://synbiohub.org/public/igem/BBa_J64018/annotation1959617/range1959617/1
type: Range
persistent identity: range1959617
display id: range1959617
version: 1
direction: direction/forward
top level: BBa_J64018
start: 1
end: 17
orientation: inline

BBa_I742145
type: ComponentDefinition
persistent identity: BBa I742145
display id: BBa_I742145
version: 1
was generated by: iGEM to SBOL conversion
description: sam5 native rbs
creator: Chris French
created: 2007-10-23T11:00:00Z
modified: 2015-08-31T04:08:03Z
discontinued: false
dominant: false
experience: experience/None
group u list: _123_
m user id: 0
owner id: 837
owning group id: 163
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is the native ribosome binding site for sam5 (4-coumarate-3-hydroxylase) of the bacterium Saccharothrix espanaensis.
mutable notes: This is a virtual part to be added to coding sequence I742141.
mutable provenance: Saccharothrix espanaensis DSM 44229
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: rbs
sequence: https://synbiohub.org/public/igem/BBa_I742145_sequence/1

rbs
type: SequenceAnnotation
persistent identity: annotation1955475
display id: annotation1955475
version: 1
top level: BBa_I742145
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_I742145/annotation1955475/range1955475/1

https://synbiohub.org/public/igem/BBa_I742145/annotation1955475/range1955475/1
type: Range
persistent identity: range1955475
display id: range1955475
version: 1
direction: direction/forward
top level: BBa_I742145
start: 3
end: 9
orientation: inline

creA RBS
type: ComponentDefinition
persistent identity: BBa J64907
display id: BBa_J64907
version: 1
was generated by: iGEM to SBOL conversion
description: creA RBS
creator: Ryan Ritterson
created: 2007-03-19T12:00:00Z
modified: 2015-05-08T01:08:17Z
discontinued: false
dominant: false
experience: experience/None
group u list: _98_
m user id: 0
owner id: 1422
owning group id: 98
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: ribosome binding site for creA gene in the E Coli cre operon
mutable notes: natural sequence, no design.
mutable provenance: E Coli strain K12
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J64907_sequence/1

BBa_J61135
type: ComponentDefinition
persistent identity: BBa J61135
display id: BBa_J61135
version: 1
was generated by: iGEM to SBOL conversion
description: Ribosome Binding Site Family Member
creator: John Anderson
created: 2007-04-24T11:00:00Z
modified: 2015-08-31T01:59:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: {{JCA_Arkin_RBSFamily}}
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J61135_sequence/1

BBa_J61128
type: ComponentDefinition
persistent identity: BBa J61128
display id: BBa_J61128
version: 1
was generated by: iGEM to SBOL conversion
description: Ribosome Binding Site Family Member
creator: John Anderson
created: 2007-04-24T11:00:00Z
modified: 2015-08-31T01:59:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable description: {{JCA_Arkin_RBSFamily}}
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J61128_sequence/1

BBa_I742146
type: ComponentDefinition
persistent identity: BBa I742146
display id: BBa_I742146
version: 1
was generated by: iGEM to SBOL conversion
description: sam8 native rbs
creator: Chris French
created: 2007-10-23T11:00:00Z
modified: 2015-08-31T04:08:03Z
discontinued: false
dominant: false
experience: experience/None
group u list: _123_
m user id: 0
owner id: 837
owning group id: 163
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Native ribosome binding site of sam8 (tyrosine ammonia lyase) from the bacterium Saccharothrix espanaensis DSM 44229.
mutable notes: Virtual part to be added to coding sequence I742142.
mutable provenance: Saccharothrix espanaensis DSM 44229.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_I742146_sequence/1

BBa_B0038
type: ComponentDefinition
persistent identity: BBa B0038
display id: BBa_B0038
version: 1
was generated by: iGEM to SBOL conversion
description: RBS 1
creator: Josh Michener
created: 2005-12-20T12:00:00Z
modified: 2015-08-31T04:07:20Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_
m user id: 0
owner id: 253
owning group id: 6
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Modified version of B0031 from the Voigt Lab. Not currently available as an independent part (only in a tet inverter).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_B0038_sequence/1

BBa_B2022
type: ComponentDefinition
persistent identity: BBa B2022
display id: BBa_B2022
version: 1
was generated by: iGEM to SBOL conversion
description: 4B RBS from T7
creator: Bartholomew Canton
created: 2007-05-22T11:00:00Z
modified: 2015-08-31T04:07:21Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_
m user id: 0
owner id: 135
owning group id: 84
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: to be added automatically later
mutable notes: to be added automatically later
mutable provenance: to be added automatically later
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_B2022_sequence/1

BBa_K1847000
type: ComponentDefinition
persistent identity: BBa K1847000
display id: BBa_K1847000
version: 1
was generated by: iGEM to SBOL conversion
description: promoter-RBS- annexin AV
creator: Anna Fomitcheva, Lisa Baumgartner
created: 2015-08-23T11:00:00Z
modified: 2015-09-17T08:44:38Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2273_
m user id: 26442
owner id: 26442
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Annexin V is member of the annexin family of Ca dependent proteins. Its function is not yet known. It binds to phosphatidylserine in a Ca-dependen fashion.
mutable notes: In combination with RBS B0032 there is no detectable expression of annexin V.
mutable provenance: Synthetic synthesis
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: Annexin V optimized for E. coli
sequence annotation: J23114
sequence annotation: B0032
sequence: https://synbiohub.org/public/igem/BBa_K1847000_sequence/1

Annexin V optimized for E. coli
type: SequenceAnnotation
persistent identity: annotation2437963
display id: annotation2437963
version: 1
top level: BBa_K1847000
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1847000/annotation2437963/range2437963/1

https://synbiohub.org/public/igem/BBa_K1847000/annotation2437963/range2437963/1
type: Range
persistent identity: range2437963
display id: range2437963
version: 1
direction: direction/forward
top level: BBa_K1847000
start: 63
end: 1025
orientation: inline

J23114
type: SequenceAnnotation
persistent identity: annotation2437961
display id: annotation2437961
version: 1
top level: BBa_K1847000
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1847000/annotation2437961/range2437961/1

https://synbiohub.org/public/igem/BBa_K1847000/annotation2437961/range2437961/1
type: Range
persistent identity: range2437961
display id: range2437961
version: 1
direction: direction/forward
top level: BBa_K1847000
start: 1
end: 35
orientation: inline

B0032
type: SequenceAnnotation
persistent identity: annotation2437962
display id: annotation2437962
version: 1
top level: BBa_K1847000
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1847000/annotation2437962/range2437962/1

https://synbiohub.org/public/igem/BBa_K1847000/annotation2437962/range2437962/1
type: Range
persistent identity: range2437962
display id: range2437962
version: 1
direction: direction/forward
top level: BBa_K1847000
start: 44
end: 56
orientation: inline

BBa_Z0262
type: ComponentDefinition
persistent identity: BBa Z0262
display id: BBa_Z0262
version: 1
was generated by: iGEM to SBOL conversion
description: Medium strength T7.2 RBS
creator: Sriram Kosuri
created: 2006-05-15T11:00:00Z
modified: 2015-05-08T01:14:55Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_10_
m user id: 0
owner id: 64
owning group id: 10
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Sequence comes from the natural B0030. Using B0030 as a medium strength RBS is from initial results from experiments characterizing all natural T7 RBSs.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_Z0262_sequence/1

BBa_J61141
type: ComponentDefinition
persistent identity: BBa J61141
display id: BBa_J61141
version: 1
was generated by: iGEM to SBOL conversion
description: {rbs1} Library Member in BBb
creator: John Anderson
created: 2007-09-03T11:00:00Z
modified: 2015-08-31T01:59:49Z
discontinued: false
dominant: false
experience: experience/None
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: {{BBbRBS1Family}}
mutable notes: {{BerkiGEM2007-Biobrick2.0Intro}}
mutable provenance: Synthetic
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J61141_sequence/1

BBa_B2040
type: ComponentDefinition
persistent identity: BBa B2040
display id: BBa_B2040
version: 1
was generated by: iGEM to SBOL conversion
description: 8 RBS from T7
creator: Bartholomew Canton
created: 2007-05-22T11:00:00Z
modified: 2015-08-31T04:07:21Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_
m user id: 0
owner id: 135
owning group id: 84
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: to be added automatically later
mutable notes: to be added automatically later
mutable provenance: to be added automatically later
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_B2040_sequence/1

T7 0.5
type: ComponentDefinition
persistent identity: BBa B2003
display id: BBa_B2003
version: 1
was generated by: iGEM to SBOL conversion
description: T7 RBS 0.5
creator: Heather Keller
created: 2006-09-27T11:00:00Z
modified: 2015-08-31T04:07:21Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_
m user id: 0
owner id: 571
owning group id: 10
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is the -20 to -1 region of T7 gp0.5, which contains the native RBS region for this gene. The physical instantiation of this part does not exist. This part is not compatible with Standard Biobricks assembly methods, as the scar created during assembly would interrupt the spacing between the RBS and the start codon of the CDS. Individuals wishing to use this RBS sequence in their assembles should refer to part B2103.
mutable notes: The -20 to -1 region was chosen to be representative of the RBS region of the T7 genes for the following reasons: 1) The Shine-Delgarno region is contained within this defined region for all T7 genes. 2) Current literature suggests that when bound to the Translation Start Site (0), the Ribosome covers positions -20 to +13 of the gene.
mutable provenance: Genome of wild-type bacteriophage T7, positions 1476-1495. Includes the -20 to -1 region of gp0.5.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: SD region
sequence: https://synbiohub.org/public/igem/BBa_B2003_sequence/1

SD region
type: SequenceAnnotation
persistent identity: annotation1902509
display id: annotation1902509
version: 1
top level: T7 0.5
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_B2003/annotation1902509/range1902509/1

https://synbiohub.org/public/igem/BBa_B2003/annotation1902509/range1902509/1
type: Range
persistent identity: range1902509
display id: range1902509
version: 1
direction: direction/forward
top level: T7 0.5
start: 13
end: 18
orientation: inline

BBa_J64028
type: ComponentDefinition
persistent identity: BBa J64028
display id: BBa_J64028
version: 1
was generated by: iGEM to SBOL conversion
description: sopB RBS
creator: Dan Widmaier
created: 2008-01-16T12:00:00Z
modified: 2015-08-31T01:56:26Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _98_
m user id: 0
owner id: 2407
owning group id: 98
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This part is the natural RBS for the sopB gene in the Salmonella Type III Secretion System.
mutable notes: This part was not designed with consideration for biobrick standard assembly in mind. This RBS hasn't been explicitly tested for expression rate in a Fluorescence or B-Gal assay.
mutable provenance: Salmonella LT2 Genomic DNA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: sopB RBS
sequence: https://synbiohub.org/public/igem/BBa_J64028_sequence/1

sopB RBS
type: SequenceAnnotation
persistent identity: annotation1959632
display id: annotation1959632
version: 1
top level: BBa_J64028
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_J64028/annotation1959632/range1959632/1

https://synbiohub.org/public/igem/BBa_J64028/annotation1959632/range1959632/1
type: Range
persistent identity: range1959632
display id: range1959632
version: 1
direction: direction/forward
top level: BBa_J64028
start: 1
end: 20
orientation: inline

BBa_M13510
type: ComponentDefinition
persistent identity: BBa M13510
display id: BBa_M13510
version: 1
was generated by: iGEM to SBOL conversion
description: M13KO7 gene X RBS
creator: Natalie Kuldell
created: 2006-12-14T12:00:00Z
modified: 2015-05-08T01:13:56Z
discontinued: false
dominant: false
experience: experience/None
group u list: _45_
m user id: 0
owner id: 314
owning group id: 1
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This RBS is part of the bacteriophage M13 genome, initiating translation of the gene X message (BBa_M13010). The part aligns with base pairs 480-495 in M13K07 genome. It was identified as the RBS for gene II message by Wezenbeek et al in Gene (1980) 11: 129-148
mutable notes: none
mutable provenance: New England Biolabs M13K07. Sequenced in 2002 by L. Panganaban
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_M13510_sequence/1

BBa_J61102
type: ComponentDefinition
persistent identity: BBa J61102
display id: BBa_J61102
version: 1
was generated by: iGEM to SBOL conversion
description: Ribosome Binding Site Family Member
creator: John Anderson
created: 2007-01-28T12:00:00Z
modified: 2015-08-31T02:03:00Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable description: {{JCA_Arkin_RBSFamily}}
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J61102_sequence/1

BBa_J34801
type: ComponentDefinition
persistent identity: BBa J34801
display id: BBa_J34801
version: 1
was generated by: iGEM to SBOL conversion
description: ribosome binding site
creator: Alexandra Choutko
created: 2006-10-25T11:00:00Z
modified: 2015-08-31T04:08:46Z
discontinued: false
dominant: false
experience: experience/None
group u list: _62_
m user id: 0
owner id: 1085
owning group id: 62
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: a
mutable notes: a
mutable provenance: a
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J34801_sequence/1

BBa_J64024
type: ComponentDefinition
persistent identity: BBa J64024
display id: BBa_J64024
version: 1
was generated by: iGEM to SBOL conversion
description: sipC RBS
creator: Dan Widmaier
created: 2008-01-16T12:00:00Z
modified: 2015-08-31T01:56:26Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _98_
m user id: 0
owner id: 2407
owning group id: 98
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This part is the natural RBS from the sipC protein in Salmonella Type III Secretion.
mutable notes: This part was not designed with biobrick standard assembly in mind. This RBS has not been explicitly measured for expression rate using fluorescence or B-Gal assay.
mutable provenance: Salmonella LT2 Genomic DNA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: sipC RBS
sequence: https://synbiohub.org/public/igem/BBa_J64024_sequence/1

sipC RBS
type: SequenceAnnotation
persistent identity: annotation1959623
display id: annotation1959623
version: 1
top level: BBa_J64024
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_J64024/annotation1959623/range1959623/1

https://synbiohub.org/public/igem/BBa_J64024/annotation1959623/range1959623/1
type: Range
persistent identity: range1959623
display id: range1959623
version: 1
direction: direction/forward
top level: BBa_J64024
start: 1
end: 15
orientation: inline

BBa_J61125
type: ComponentDefinition
persistent identity: BBa J61125
display id: BBa_J61125
version: 1
was generated by: iGEM to SBOL conversion
description: Ribosome Binding Site Family Member
creator: John Anderson
created: 2007-04-24T11:00:00Z
modified: 2015-08-31T01:59:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable description: {{JCA_Arkin_RBSFamily}}
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J61125_sequence/1

BBa_J61139
type: ComponentDefinition
persistent identity: BBa J61139
display id: BBa_J61139
version: 1
was generated by: iGEM to SBOL conversion
description: Ribosome Binding Site Family Member
creator: John Anderson
created: 2007-04-24T11:00:00Z
modified: 2015-08-31T01:59:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable description: {{JCA_Arkin_RBSFamily}}
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J61139_sequence/1

BBa_K082001
type: ComponentDefinition
persistent identity: BBa K082001
display id: BBa_K082001
version: 1
was generated by: iGEM to SBOL conversion
description: RBS medium
creator: Yanting Xue , Binjie Xu
created: 2008-10-27T12:00:00Z
modified: 2015-05-08T01:08:35Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _228_
m user id: 0
owner id: 1560
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Weak1 RBS based on Ron Weiss thesis. Strength is considered relative to BBa_B0030, BBa_B0031, BBa_B0033.
mutable notes: the
mutable provenance: the
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: BBa_K082001
sequence: https://synbiohub.org/public/igem/BBa_K082001_sequence/1

BBa_K082001
type: SequenceAnnotation
persistent identity: annotation1993438
display id: annotation1993438
version: 1
top level: BBa_K082001
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K082001/annotation1993438/range1993438/1

https://synbiohub.org/public/igem/BBa_K082001/annotation1993438/range1993438/1
type: Range
persistent identity: range1993438
display id: range1993438
version: 1
direction: direction/reverse
top level: BBa_K082001
start: 1
end: 15
orientation: inline

BBa_K1895001
type: ComponentDefinition
persistent identity: BBa K1895001
display id: BBa_K1895001
version: 1
was generated by: iGEM to SBOL conversion
description: rpoH
creator: Lauren Mills
created: 2016-07-07T11:00:00Z
modified: 2016-08-03T07:04:22Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2361_
m user id: 31559
owner id: 31475
owning group id: 9
status: status/Planning
bookmark: false
mutable description: wefWD
mutable notes: awefWv
mutable provenance: AWwvf
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_K1895001_sequence/1

RBS-spoVG
type: ComponentDefinition
persistent identity: BBa K143021
display id: BBa_K143021
version: 1
was generated by: iGEM to SBOL conversion
description: SpoVG ribosome binding site (RBS) for B. subtilis
creator: James Chappell
created: 2008-09-16T11:00:00Z
modified: 2015-05-08T01:10:23Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _199_
m user id: 0
owner id: 2090
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Description: SpoVG is an endogenous ribosome binding site from B.subtilis. The sequence of the spoVG ribosome binding site is AAAGGUGGUGA which is complementary to the sequence UUUCCUCCACU from the 3' region of the 16s rRNA from B.subtilis. Previous research showed that the predicted binding energy of the 16s rRNA to the RBS is -19kcal 1
mutable notes: In order to ensure that the RBS is functional the actual ribosome binding site was maintained and the distance between the RBS and the start codon maintained. In order to conform to the biobrick standard the sequence flanking the RBS had to be changed but the distance between the promoter and RBS, and start codon and RBS was maintained.
mutable provenance: The sequence was taken from a previous research paper [1] and was constructed by Geneart.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: rbs
sequence: https://synbiohub.org/public/igem/BBa_K143021_sequence/1

rbs
type: SequenceAnnotation
persistent identity: annotation1975997
display id: annotation1975997
version: 1
top level: RBS-spoVG
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K143021/annotation1975997/range1975997/1

https://synbiohub.org/public/igem/BBa_K143021/annotation1975997/range1975997/1
type: Range
persistent identity: range1975997
display id: range1975997
version: 1
direction: direction/forward
top level: RBS-spoVG
start: 1
end: 12
orientation: inline

BBa_J61105
type: ComponentDefinition
persistent identity: BBa J61105
display id: BBa_J61105
version: 1
was generated by: iGEM to SBOL conversion
description: Ribosome Binding Site Family Member
creator: John Anderson
created: 2007-04-23T11:00:00Z
modified: 2015-08-31T02:03:00Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable description: {{JCA_Arkin_RBSFamily}}
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J61105_sequence/1

BBa_J61130
type: ComponentDefinition
persistent identity: BBa J61130
display id: BBa_J61130
version: 1
was generated by: iGEM to SBOL conversion
description: Ribosome Binding Site Family Member
creator: John Anderson
created: 2007-04-24T11:00:00Z
modified: 2015-08-31T01:59:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable description: {{JCA_Arkin_RBSFamily}}
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J61130_sequence/1

BBa_B0073
type: ComponentDefinition
persistent identity: BBa B0073
display id: BBa_B0073
version: 1
was generated by: iGEM to SBOL conversion
description: Specialized RBS
creator: Bartholomew Canton
created: 2006-05-01T11:00:00Z
modified: 2015-08-31T04:07:21Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_6_
m user id: 0
owner id: 135
owning group id: 6
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_B0073_sequence/1

BBa_J61136
type: ComponentDefinition
persistent identity: BBa J61136
display id: BBa_J61136
version: 1
was generated by: iGEM to SBOL conversion
description: Ribosome Binding Site Family Member
creator: John Anderson
created: 2007-04-24T11:00:00Z
modified: 2015-08-31T01:59:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable description: {{JCA_Arkin_RBSFamily}}
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J61136_sequence/1

BBa_B0075
type: ComponentDefinition
persistent identity: BBa B0075
display id: BBa_B0075
version: 1
was generated by: iGEM to SBOL conversion
description: Orthogonal RBS
creator: Felix Moser
created: 2008-01-17T12:00:00Z
modified: 2015-08-31T04:07:21Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_
m user id: 0
owner id: 2398
owning group id: 11
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Predicted by Lon Chubiz to be RBS sequence with strongest affinity for T7 ribosomes.
mutable notes: initial AA may be unnecessary
mutable provenance: Lon Chubiz
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: rbs
sequence: https://synbiohub.org/public/igem/BBa_B0075_sequence/1

rbs
type: SequenceAnnotation
persistent identity: annotation1959963
display id: annotation1959963
version: 1
top level: BBa_B0075
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_B0075/annotation1959963/range1959963/1

https://synbiohub.org/public/igem/BBa_B0075/annotation1959963/range1959963/1
type: Range
persistent identity: range1959963
display id: range1959963
version: 1
direction: direction/forward
top level: BBa_B0075
start: 1
end: 8
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

BBa_B0076
type: ComponentDefinition
persistent identity: BBa B0076
display id: BBa_B0076
version: 1
was generated by: iGEM to SBOL conversion
description: Specialized RBS
creator: Bartholomew Canton
created: 2006-05-01T11:00:00Z
modified: 2015-08-31T04:07:21Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_6_
m user id: 0
owner id: 135
owning group id: 6
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_B0076_sequence/1

BBa_J61133
type: ComponentDefinition
persistent identity: BBa J61133
display id: BBa_J61133
version: 1
was generated by: iGEM to SBOL conversion
description: Ribosome Binding Site Family Member
creator: John Anderson
created: 2007-04-24T11:00:00Z
modified: 2015-08-31T01:59:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable description: {{JCA_Arkin_RBSFamily}}
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J61133_sequence/1

BBa_J61108
type: ComponentDefinition
persistent identity: BBa J61108
display id: BBa_J61108
version: 1
was generated by: iGEM to SBOL conversion
description: Ribosome Binding Site Family Member
creator: John Anderson
created: 2007-04-23T11:00:00Z
modified: 2015-08-31T02:03:00Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable description: {{JCA_Arkin_RBSFamily}}
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J61108_sequence/1

BBa_J15001
type: ComponentDefinition
persistent identity: BBa J15001
display id: BBa_J15001
version: 1
was generated by: iGEM to SBOL conversion
description: strong synthetic E. coli ribosome binding site with SacI site.
creator: Chris French
created: 2007-07-12T11:00:00Z
modified: 2015-08-31T04:08:32Z
discontinued: false
dominant: false
experience: experience/None
group u list: _163_
m user id: 0
owner id: 837
owning group id: 163
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is a strong synthetic E. coli ribosome binding site. It is synthesised as two complementary oligonucleotides rather than being incorporated into a biobrick plasmid. It incorporates a SacI site overlapping the XbaI site, which is preserved when it is added to any other biobrick. This allows easy detection of the RBS after it has been added upstream of a biobrick coding sequence in a plasmid vector.
mutable notes: Note the presence of a SacI site overlapping the XbaI site, which is preserved when this biobrick is added to any other biobrick. At the time of writing, this biobrick is added as a short piece of DNA composed of two complementary oligonucleotides rather than being incorporated into a biobrick cloning vector by itself. It can be added upstream of a biobrick coding sequence, and its presence can easily be detected in miniprep DNA on a gel by using a SacI-SpeI or similar digest.
mutable provenance: Synthetic.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: SacI
sequence annotation: rbs
sequence: https://synbiohub.org/public/igem/BBa_J15001_sequence/1

SacI
type: SequenceAnnotation
persistent identity: annotation1938045
display id: annotation1938045
version: 1
top level: BBa_J15001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J15001/annotation1938045/range1938045/1

https://synbiohub.org/public/igem/BBa_J15001/annotation1938045/range1938045/1
type: Range
persistent identity: range1938045
display id: range1938045
version: 1
direction: direction/forward
top level: BBa_J15001
start: 1
end: 3
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation1938046
display id: annotation1938046
version: 1
top level: BBa_J15001
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_J15001/annotation1938046/range1938046/1

https://synbiohub.org/public/igem/BBa_J15001/annotation1938046/range1938046/1
type: Range
persistent identity: range1938046
display id: range1938046
version: 1
direction: direction/forward
top level: BBa_J15001
start: 4
end: 10
orientation: inline

BBa_J61145
type: ComponentDefinition
persistent identity: BBa J61145
display id: BBa_J61145
version: 1
was generated by: iGEM to SBOL conversion
description: {rbs1} Library Member in BBb
creator: John Anderson
created: 2007-09-03T11:00:00Z
modified: 2015-08-31T01:56:25Z
discontinued: false
dominant: false
experience: experience/None
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: {{BBbRBS1Family}}
mutable notes: {{BerkiGEM2007-Biobrick2.0Intro}}
mutable provenance: Synthetic
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J61145_sequence/1

BBa_B0041
type: ComponentDefinition
persistent identity: BBa B0041
display id: BBa_B0041
version: 1
was generated by: iGEM to SBOL conversion
description: RBS 3
creator: Josh Michener
created: 2005-12-20T12:00:00Z
modified: 2015-08-31T04:07:20Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_
m user id: 0
owner id: 253
owning group id: 6
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Modified version of B0031 from the Voigt Lab. Not currently available as an independent part (only in a tet inverter).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_B0041_sequence/1

BBa_J61129
type: ComponentDefinition
persistent identity: BBa J61129
display id: BBa_J61129
version: 1
was generated by: iGEM to SBOL conversion
description: Ribosome Binding Site Family Member
creator: John Anderson
created: 2007-04-24T11:00:00Z
modified: 2015-08-31T01:59:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable description: {{JCA_Arkin_RBSFamily}}
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J61129_sequence/1

BBa_J61118
type: ComponentDefinition
persistent identity: BBa J61118
display id: BBa_J61118
version: 1
was generated by: iGEM to SBOL conversion
description: Ribosome Binding Site Family Member
creator: John Anderson
created: 2007-04-23T11:00:00Z
modified: 2015-08-31T01:59:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable description: {{JCA_Arkin_RBSFamily}}
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J61118_sequence/1

BBa_J64031
type: ComponentDefinition
persistent identity: BBa J64031
display id: BBa_J64031
version: 1
was generated by: iGEM to SBOL conversion
description: sptP RBS
creator: Dan Widmaier
created: 2008-01-16T12:00:00Z
modified: 2015-08-31T01:56:26Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _98_
m user id: 0
owner id: 2407
owning group id: 98
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is the natural RBS for the sptP gene in Salmonella Type III Secretion
mutable notes: This part was designed without consideration for biobrick standard assembly. This RBS has not been explicitly tested for expression rate.
mutable provenance: Salmonella LT2 Genomic DNA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: sptP RBS
sequence: https://synbiohub.org/public/igem/BBa_J64031_sequence/1

sptP RBS
type: SequenceAnnotation
persistent identity: annotation1959635
display id: annotation1959635
version: 1
top level: BBa_J64031
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_J64031/annotation1959635/range1959635/1

https://synbiohub.org/public/igem/BBa_J64031/annotation1959635/range1959635/1
type: Range
persistent identity: range1959635
display id: range1959635
version: 1
direction: direction/forward
top level: BBa_J64031
start: 1
end: 20
orientation: inline

BBa_J61113
type: ComponentDefinition
persistent identity: BBa J61113
display id: BBa_J61113
version: 1
was generated by: iGEM to SBOL conversion
description: Ribosome Binding Site Family Member
creator: John Anderson
created: 2007-04-23T11:00:00Z
modified: 2015-08-31T02:03:00Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable description: {{JCA_Arkin_RBSFamily}}
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J61113_sequence/1

BBa_I742163
type: ComponentDefinition
persistent identity: BBa I742163
display id: BBa_I742163
version: 1
was generated by: iGEM to SBOL conversion
description: native ftsK rbs
creator: Chris French
created: 2007-10-24T11:00:00Z
modified: 2015-08-31T04:08:03Z
discontinued: false
dominant: false
experience: experience/None
group u list: _123_
m user id: 0
owner id: 837
owning group id: 163
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is the native ribosome binding site of ftsK, a DNA-translocating motor protein involved in cell division in Escherichia coli.
mutable notes: Virtual part to be added to coding sequence BBa_I742137.
mutable provenance: Escherichia coli JM109 (a K12 strain).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: SacI
sequence annotation: rbs
sequence: https://synbiohub.org/public/igem/BBa_I742163_sequence/1

SacI
type: SequenceAnnotation
persistent identity: annotation1954640
display id: annotation1954640
version: 1
top level: BBa_I742163
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I742163/annotation1954640/range1954640/1

https://synbiohub.org/public/igem/BBa_I742163/annotation1954640/range1954640/1
type: Range
persistent identity: range1954640
display id: range1954640
version: 1
direction: direction/forward
top level: BBa_I742163
start: 1
end: 3
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation1954641
display id: annotation1954641
version: 1
top level: BBa_I742163
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_I742163/annotation1954641/range1954641/1

https://synbiohub.org/public/igem/BBa_I742163/annotation1954641/range1954641/1
type: Range
persistent identity: range1954641
display id: range1954641
version: 1
direction: direction/forward
top level: BBa_I742163
start: 15
end: 20
orientation: inline

BBa_M13504
type: ComponentDefinition
persistent identity: BBa M13504
display id: BBa_M13504
version: 1
was generated by: iGEM to SBOL conversion
description: M13K07 gene IV RBS
creator: Natalie Kuldell
created: 2006-12-14T12:00:00Z
modified: 2015-05-08T01:13:56Z
discontinued: false
dominant: false
experience: experience/None
group u list: _45_
m user id: 0
owner id: 314
owning group id: 1
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This RBS is part of the bacteriophage M13 genome, initiating translation of the gene IV message (BBa_M13004). The part aligns with base pairs 4204-4219 in M13K07 genome. It was identified as the RBS for gene IV message by Wezenbeek et al in Gene (1980) 11: 129-148
mutable notes: none
mutable provenance: New England Biolabs M13K07. Sequenced in 2002 by L. Panganaban
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_M13504_sequence/1

BBa_B2017
type: ComponentDefinition
persistent identity: BBa B2017
display id: BBa_B2017
version: 1
was generated by: iGEM to SBOL conversion
description: 2.8 RBS from T7
creator: Bartholomew Canton
created: 2007-05-22T11:00:00Z
modified: 2015-08-31T04:07:21Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_
m user id: 0
owner id: 135
owning group id: 84
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: to be added automatically later
mutable notes: to be added automatically later
mutable provenance: to be added automatically later
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_B2017_sequence/1

BBa_J61101
type: ComponentDefinition
persistent identity: BBa J61101
display id: BBa_J61101
version: 1
was generated by: iGEM to SBOL conversion
description: Ribosome Binding Site Family Member
creator: John Anderson
created: 2007-01-28T12:00:00Z
modified: 2015-08-31T02:03:00Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable description: fix
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J61101_sequence/1

BBa_K118012
type: ComponentDefinition
persistent identity: BBa K118012
display id: BBa_K118012
version: 1
was generated by: iGEM to SBOL conversion
description: Synthetic ribosome binding site added by Son of Babel procedure
creator: Andrew Hall
created: 2008-08-18T11:00:00Z
modified: 2015-05-08T01:09:36Z
discontinued: false
dominant: false
experience: experience/None
group u list: _192_
m user id: 0
owner id: 3282
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is a 4bp synthetic ribosome binding site which can be added by fusion PCR using a reverse primer and the Son of Babel cloning system.
mutable notes: The part can be added by fusion PCR using a Son of Babel reverse primer.
mutable provenance: Synthetic DNA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_K118012_sequence/1

BBa_J64022
type: ComponentDefinition
persistent identity: BBa J64022
display id: BBa_J64022
version: 1
was generated by: iGEM to SBOL conversion
description: sicA RBS
creator: Dan Widmaier
created: 2008-01-16T12:00:00Z
modified: 2015-08-31T01:56:26Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _98_
m user id: 0
owner id: 2407
owning group id: 98
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is the natural RBS from the sicA chaperone (BBa_J6021) in the Salmonella Type III Secretion System.
mutable notes: This part was not designed with standard biobrick assembly in mind.
mutable provenance: Salmonella LT2 Genomic DNA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: misc
sequence: https://synbiohub.org/public/igem/BBa_J64022_sequence/1

misc
type: SequenceAnnotation
persistent identity: annotation1959621
display id: annotation1959621
version: 1
top level: BBa_J64022
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J64022/annotation1959621/range1959621/1

https://synbiohub.org/public/igem/BBa_J64022/annotation1959621/range1959621/1
type: Range
persistent identity: range1959621
display id: range1959621
version: 1
direction: direction/forward
top level: BBa_J64022
start: 1
end: 20
orientation: inline

BBa_I723019
type: ComponentDefinition
persistent identity: BBa I723019
display id: BBa_I723019
version: 1
was generated by: iGEM to SBOL conversion
description: RBS for XylR
creator: Scott Ramsay
created: 2007-10-24T11:00:00Z
modified: 2015-08-31T04:07:54Z
discontinued: false
dominant: false
experience: experience/None
group u list: _126_
m user id: 0
owner id: 2140
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is the naturally-occurring RBS found in the regulatory region of the DntR gene. gataaaaacaagaggaaaacaa
mutable notes: [Chistine/Maia]
mutable provenance: [Christine/Maia]
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_I723019_sequence/1

RBS
type: SequenceAnnotation
persistent identity: annotation1955263
display id: annotation1955263
version: 1
top level: BBa_I723019
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_I723019/annotation1955263/range1955263/1

https://synbiohub.org/public/igem/BBa_I723019/annotation1955263/range1955263/1
type: Range
persistent identity: range1955263
display id: range1955263
version: 1
direction: direction/forward
top level: BBa_I723019
start: 1
end: 20
orientation: inline

PnisA
type: ComponentDefinition
persistent identity: BBa K1830001
display id: BBa_K1830001
version: 1
was generated by: iGEM to SBOL conversion
description: PnisA
created: 2015-08-05T11:00:00Z
modified: 2015-08-07T11:50:06Z
mutable provenance: L. Lactis NisA operon
owned by: james
owned by: myers
mutable description: Promoter and ribosome binding site of the Lactococcal nisin inducible operon.
discontinued: false
dominant: false
experience: experience/None
group u list: _2256_
m user id: 27439
owner id: 27439
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Small part
star: false
creator: Mathieu Husser
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -35
sequence annotation: RBS
sequence annotation: PnisA
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_K1830001_sequence/1

-35
type: SequenceAnnotation
persistent identity: annotation2434539
display id: annotation2434539
version: 1
top level: PnisA
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K1830001/annotation2434539/range2434539/1

https://synbiohub.org/public/igem/BBa_K1830001/annotation2434539/range2434539/1
type: Range
persistent identity: range2434539
display id: range2434539
version: 1
top level: PnisA
direction: direction/forward
start: 97
end: 102
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2434538
display id: annotation2434538
version: 1
top level: PnisA
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1830001/annotation2434538/range2434538/1

https://synbiohub.org/public/igem/BBa_K1830001/annotation2434538/range2434538/1
type: Range
persistent identity: range2434538
display id: range2434538
version: 1
top level: PnisA
direction: direction/forward
start: 159
end: 165
orientation: inline

PnisA
type: SequenceAnnotation
persistent identity: annotation2434537
display id: annotation2434537
version: 1
top level: PnisA
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1830001/annotation2434537/range2434537/1

https://synbiohub.org/public/igem/BBa_K1830001/annotation2434537/range2434537/1
type: Range
persistent identity: range2434537
display id: range2434537
version: 1
top level: PnisA
direction: direction/forward
start: 1
end: 173
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2434540
display id: annotation2434540
version: 1
top level: PnisA
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K1830001/annotation2434540/range2434540/1

https://synbiohub.org/public/igem/BBa_K1830001/annotation2434540/range2434540/1
type: Range
persistent identity: range2434540
display id: range2434540
version: 1
top level: PnisA
direction: direction/forward
start: 120
end: 125
orientation: inline

BBa_J56013
type: ComponentDefinition
persistent identity: BBa J56013
display id: BBa_J56013
version: 1
was generated by: iGEM to SBOL conversion
description: Rbs2 ribosome binding site
creator: Matt Eames
created: 2006-10-31T12:00:00Z
modified: 2015-08-31T03:22:59Z
discontinued: false
dominant: false
experience: experience/None
group u list: _79_98_
m user id: 0
owner id: 1132
owning group id: 98
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: ribosome binding site for transcription initiation
mutable notes: None
mutable provenance: Synthesized by DNA 2.0
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J56013_sequence/1

BBa_J64015
type: ComponentDefinition
persistent identity: BBa J64015
display id: BBa_J64015
version: 1
was generated by: iGEM to SBOL conversion
description: sopA RBS
creator: Dan Widmaier
created: 2008-01-16T12:00:00Z
modified: 2015-08-31T01:56:26Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _98_
m user id: 0
owner id: 2407
owning group id: 98
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is the natural RBS associated with the sopA effector protein in the Salmonella SPI-1 system.
mutable notes: This part was not designed with compliance to biobrick standard assembly in mind. This RBS hasn't been explicitly tested in an expression assay such as GFP fluorescence or B-Gal expression.
mutable provenance: Salmonella LT2 Genomic DNA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: sopA RBS
sequence: https://synbiohub.org/public/igem/BBa_J64015_sequence/1

sopA RBS
type: SequenceAnnotation
persistent identity: annotation1959614
display id: annotation1959614
version: 1
top level: BBa_J64015
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_J64015/annotation1959614/range1959614/1

https://synbiohub.org/public/igem/BBa_J64015/annotation1959614/range1959614/1
type: Range
persistent identity: range1959614
display id: range1959614
version: 1
direction: direction/forward
top level: BBa_J64015
start: 1
end: 16
orientation: inline

BBa_B0036
type: ComponentDefinition
persistent identity: BBa B0036
display id: BBa_B0036
version: 1
was generated by: iGEM to SBOL conversion
description: Specialized RBS
creator: Barry Canton
created: 2004-06-07T11:00:00Z
modified: 2015-08-31T04:07:20Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_6_
m user id: 0
owner id: 135
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This RBS is recognized by ribosomes carrying the modified anti-Shine Dalgarno sequence as found in Brink et al. (see below)
mutable provenance: Brink MF, Verbeet MP, de Boer HA (1995). Specialized ribosomes: highly specific translation in vivo of a single targetted mRNA species. Gene, 156, pp. 215-222
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_B0036_sequence/1

trkD
type: ComponentDefinition
persistent identity: BBa K629005
display id: BBa_K629005
version: 1
was generated by: iGEM to SBOL conversion
description: trkD, a functional Kup (formerly TrkD) system took up Cs+ with a moderate rate and affinity
creator: Zilong WANG, Yi ZHENG
created: 2011-10-03T11:00:00Z
modified: 2015-05-08T01:12:56Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _801_
m user id: 0
owner id: 9022
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: Escherichia coli cells which contain a functional Kup (formerly TrkD) system took up Cs' with a moderate rate and affinity. Kup is a separate K+ uptake system with relatively little discrimination in the transport of the cations K+, Rb+, and Cs'. Regardless of the presence or absence of Kup, K+-replete cells took up Cs'primarily by a very low affinity mode, proportional to the ratio of the Cs' and K+ concentrations in the medium. In our project, trkD is used to collect Cs-137 when recN is activated by radiation and start the transcription of trkD,in order to absorb this kind of radioactive source like Cs-137
mutable notes: none
mutable provenance: ATGAGCACTGATAATAAGCAATCATTGCCCGCGATTACCCTCGCGGCGATTGGAGTTGTCTACGGCGATATTGGTACCAGCCCGTTATATACACTTCGTGAATGTTTGTCCGGCCAGTTTGGTTTTGGCGTTGAACGCGATGCCGTGTTTGGCTTTTTATCGCTGATCTTCTGGCTGCTAATCTTTGTGGTTTCCATTAAATATCTCACCTTCGTGATGCGGGCAGATAACGCCGGTGAGGGGGGGATCCTGACGTTGATGTCGCTTGCCGGGCGTAATACGTCGGCGCGAACCACATCAATGCTGGTGATTATGGGGCTAATCGGCGGCAGCTTTTTCTATGGTGAAGTCGTCATAACACCCGCTATTTCGGTGATGTCAGCCATTGAAGGTCTGGAAATCGTCGCCCCGCAGCTGGATACCTGGATAGTTCCCCTCTCAATTATCGTTCTCACTTTATTATTTATGATTCAAAAGCATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGTGCTGCATGCGCTGAATCCGATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGCTGTCGATTACGGGGGTCGAGGCGCTGTATGCTGATATGGGACACTTTGGTAAGTTCCCTATTCGTCTGGCGTGGTTCACTGTCGTATTACCTTCCTTAACTCTTAATTACTTCGGCCAGGGAGCGCTGTTGTTAAAGAACCCGGAAGCGATTAAGAACCCGTTCTTCCTTTTGGCACCGGACTGGGCGCTGATCCCGCTGCTGATCATCGCCGCACTGGCGACGGTAATCGCCTCGCAGGCGGTTATCTCTGGCGTCTTTTCATTGACGCGTCAGGCGGTACGTCTGGGATATTTGTCGCCGATGCGCATTATTCACACCTCCGAAATGGAGTCAGGGCAAATCTATATTCCGTTTGTGAACTGGATGCTCTATGTCGCGGTCGTGATTGTGATTGTCAGCTTTGAGCACTCCAGCAACCTGGCGGCGGCGTACGGGATTGCGGTGACCGGAACCATGGTGCTGACGTCTATTCTCTCGACTACCGTGGCACGTCAGAACTGGCACTGGAATAAGTATTTTGTTGCGCTGATCCTGATTGCTTTCCTTTGTGTCGATATTCCATTGTTCACCGCTAACCTCGATAAACTGCTCTCCGGCGGCTGGTTGCCATTGAGCCTCGGTACTGTGATGTTTATCGTGATGACCACCTGGAAGAGCGAGCGTTTCCGCTTGCTGCGGCGGATGCATGAACATGGTAACTCTCTGGAAGCGATGATTGCTTCGCTGGAGAAATCACCGCCTGTTCGCGTGCCCGGGACCGCGGTGTATATGTCGCGTGCAATCAACGTCATTCCCTTTGCGCTGATGCATAACCTTAAACATAACAAGGTATTGCATGAGCGGGTGATTCTGTTAACTCTGCGCACCGAAGACGCTCCATATGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTCGAAATCTAA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: TrkD
sequence annotation: start
sequence annotation: fwd primer
sequence annotation: rev primer
sequence annotation: terminator
sequence: https://synbiohub.org/public/igem/BBa_K629005_sequence/1

TrkD
type: SequenceAnnotation
persistent identity: annotation2157756
display id: annotation2157756
version: 1
top level: trkD
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K629005/annotation2157756/range2157756/1

https://synbiohub.org/public/igem/BBa_K629005/annotation2157756/range2157756/1
type: Range
persistent identity: range2157756
display id: range2157756
version: 1
direction: direction/forward
top level: trkD
start: 1
end: 1869
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2157754
display id: annotation2157754
version: 1
top level: trkD
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K629005/annotation2157754/range2157754/1

https://synbiohub.org/public/igem/BBa_K629005/annotation2157754/range2157754/1
type: Range
persistent identity: range2157754
display id: range2157754
version: 1
direction: direction/forward
top level: trkD
start: 1
end: 3
orientation: inline

fwd primer
type: SequenceAnnotation
persistent identity: annotation2157755
display id: annotation2157755
version: 1
top level: trkD
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K629005/annotation2157755/range2157755/1

https://synbiohub.org/public/igem/BBa_K629005/annotation2157755/range2157755/1
type: Range
persistent identity: range2157755
display id: range2157755
version: 1
direction: direction/forward
top level: trkD
start: 1
end: 21
orientation: inline

rev primer
type: SequenceAnnotation
persistent identity: annotation2157757
display id: annotation2157757
version: 1
top level: trkD
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K629005/annotation2157757/range2157757/1

https://synbiohub.org/public/igem/BBa_K629005/annotation2157757/range2157757/1
type: Range
persistent identity: range2157757
display id: range2157757
version: 1
direction: direction/reverse
top level: trkD
start: 1849
end: 1869
orientation: inline

terminator
type: SequenceAnnotation
persistent identity: annotation2157758
display id: annotation2157758
version: 1
top level: trkD
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K629005/annotation2157758/range2157758/1

https://synbiohub.org/public/igem/BBa_K629005/annotation2157758/range2157758/1
type: Range
persistent identity: range2157758
display id: range2157758
version: 1
direction: direction/forward
top level: trkD
start: 1867
end: 1869
orientation: inline

BBa_J61123
type: ComponentDefinition
persistent identity: BBa J61123
display id: BBa_J61123
version: 1
was generated by: iGEM to SBOL conversion
description: Ribosome Binding Site Family Member
creator: John Anderson
created: 2007-04-24T11:00:00Z
modified: 2015-08-31T01:59:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable description: {{JCA_Arkin_RBSFamily}}
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J61123_sequence/1

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: stop
sequence annotation: T7 TE
sequence annotation: polya
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

BBa_B0074
type: ComponentDefinition
persistent identity: BBa B0074
display id: BBa_B0074
version: 1
was generated by: iGEM to SBOL conversion
description: Orthogonal RBS
creator: Bartholomew Canton
created: 2006-05-01T11:00:00Z
modified: 2015-08-31T04:07:21Z
discontinued: false
dominant: true
experience: experience/None
group u list: _11_6_
m user id: 0
owner id: 135
owning group id: 6
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_B0074_sequence/1

BBa_J61137
type: ComponentDefinition
persistent identity: BBa J61137
display id: BBa_J61137
version: 1
was generated by: iGEM to SBOL conversion
description: Ribosome Binding Site Family Member
creator: John Anderson
created: 2007-04-24T11:00:00Z
modified: 2015-08-31T01:59:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable description: {{JCA_Arkin_RBSFamily}}
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J61137_sequence/1

BBa_K1675006
type: ComponentDefinition
persistent identity: BBa K1675006
display id: BBa_K1675006
version: 1
was generated by: iGEM to SBOL conversion
description: J23119+B0034+NhaA+B0034+NhaB
creator: JIAQI XU
created: 2015-09-10T11:00:00Z
modified: 2015-09-12T12:51:52Z
discontinued: false
dominant: false
group u list: _2093_
m user id: 27740
owner id: 27740
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Construct the JBA (J23119+B0034+NhaA), B0034 and NhaB with the vector pSB1C3. Two functional genes NhaA and NhaB are used to pump in protons and lower the pH of bacteria???s microenvironment. JBA and JBB are linked with pSB1C3 through 3A Assemble successfully. Thus, it can be an alkaline-resisting part.
mutable notes: This is a composite part including the J23119 promoter ecoded in part BBa_J23119 and the ribosome binding site B0034 ecoded in part BBa_B0034. The functional genes NhaA and NhaB are cloned from Escherichia coli str. K-12 substr. sequence found through Genbank http://www.ncbi.nlm.nih.gov/nuccore/NC_000913.3?report=genbank&from=17489&to=18655 http://www.ncbi.nlm.nih.gov/nuccore/NC_000913.3?report=genbank&from=1233176&to=1234717&strand=true
mutable provenance: The sequence of promoter J23119 is found through the standard part BBa_J23119 http://parts.igem.org/Part:BBa_J23119 The sequence of ribosome binding site B0034 is found through the standard part BBa_B0034 http://parts.igem.org/Part:BBa_B0034 The sequence of gene NhaA is found through Genbank http://www.ncbi.nlm.nih.gov/nuccore/NC_000913.3?report=genbank&from=17489&to=18655 The sequence of gene NhaB is found through Genbank http://www.ncbi.nlm.nih.gov/nuccore/NC_000913.3?report=genbank&from=1233176&to=1234717&strand=true
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0034
component: BBa_K1675000
component: BBa_K1675001
component: BBa_J23119
sequence annotation: BBa_K1675000
sequence annotation: BBa_B0034
sequence annotation: BBa_K1675001
sequence annotation: BBa_J23119
sequence: https://synbiohub.org/public/igem/BBa_K1675006_sequence/1

BBa_B0034
type: Component
persistent identity: component2449070
display id: component2449070
version: 1
top level: BBa_K1675006
access: public
definition: BBa_B0034

BBa_K1675000
type: Component
persistent identity: component2449068
display id: component2449068
version: 1
top level: BBa_K1675006
access: public
definition: BBa_K1675000

BBa_K1675001
type: Component
persistent identity: component2449083
display id: component2449083
version: 1
top level: BBa_K1675006
access: public
definition: BBa_K1675001

BBa_J23119
type: Component
persistent identity: component2449062
display id: component2449062
version: 1
top level: BBa_K1675006
access: public
definition: BBa_J23119

BBa_B0034
type: Component
persistent identity: component2449064
display id: component2449064
version: 1
top level: BBa_K1675006
access: public
definition: BBa_B0034

BBa_K1675000
type: SequenceAnnotation
persistent identity: annotation2449068
display id: annotation2449068
version: 1
top level: BBa_K1675006
location: https://synbiohub.org/public/igem/BBa_K1675006/annotation2449068/range2449068/1
component: BBa_K1675000

https://synbiohub.org/public/igem/BBa_K1675006/annotation2449068/range2449068/1
type: Range
persistent identity: range2449068
display id: range2449068
version: 1
direction: direction/reverse
top level: BBa_K1675006
start: 64
end: 1230
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2449070
display id: annotation2449070
version: 1
top level: BBa_K1675006
location: https://synbiohub.org/public/igem/BBa_K1675006/annotation2449070/range2449070/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1675006/annotation2449070/range2449070/1
type: Range
persistent identity: range2449070
display id: range2449070
version: 1
direction: direction/reverse
top level: BBa_K1675006
start: 1239
end: 1250
orientation: inline

BBa_K1675001
type: SequenceAnnotation
persistent identity: annotation2449083
display id: annotation2449083
version: 1
top level: BBa_K1675006
location: https://synbiohub.org/public/igem/BBa_K1675006/annotation2449083/range2449083/1
component: BBa_K1675001

https://synbiohub.org/public/igem/BBa_K1675006/annotation2449083/range2449083/1
type: Range
persistent identity: range2449083
display id: range2449083
version: 1
direction: direction/reverse
top level: BBa_K1675006
start: 1257
end: 2798
orientation: inline

BBa_J23119
type: SequenceAnnotation
persistent identity: annotation2449062
display id: annotation2449062
version: 1
top level: BBa_K1675006
location: https://synbiohub.org/public/igem/BBa_K1675006/annotation2449062/range2449062/1
component: BBa_J23119

https://synbiohub.org/public/igem/BBa_K1675006/annotation2449062/range2449062/1
type: Range
persistent identity: range2449062
display id: range2449062
version: 1
direction: direction/reverse
top level: BBa_K1675006
start: 1
end: 35
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2449064
display id: annotation2449064
version: 1
top level: BBa_K1675006
location: https://synbiohub.org/public/igem/BBa_K1675006/annotation2449064/range2449064/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1675006/annotation2449064/range2449064/1
type: Range
persistent identity: range2449064
display id: range2449064
version: 1
direction: direction/reverse
top level: BBa_K1675006
start: 44
end: 55
orientation: inline

BBa_K082000
type: ComponentDefinition
persistent identity: BBa K082000
display id: BBa_K082000
version: 1
was generated by: iGEM to SBOL conversion
description: reconstruct strong RBS
creator: Yanting Xue , Binjie Xu
created: 2008-10-27T12:00:00Z
modified: 2015-05-08T01:08:35Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _228_
m user id: 0
owner id: 1560
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Strong RBS based on Ron Weiss thesis. Strength is considered relative to BBa_B0031, BBa_B0032, BBa_B0033 and BBa_B0034.
mutable notes: The
mutable provenance: The
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: BBa_K082000
sequence: https://synbiohub.org/public/igem/BBa_K082000_sequence/1

BBa_K082000
type: SequenceAnnotation
persistent identity: annotation1992768
display id: annotation1992768
version: 1
top level: BBa_K082000
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K082000/annotation1992768/range1992768/1

https://synbiohub.org/public/igem/BBa_K082000/annotation1992768/range1992768/1
type: Range
persistent identity: range1992768
display id: range1992768
version: 1
direction: direction/reverse
top level: BBa_K082000
start: 1
end: 17
orientation: inline

BBa_J61146
type: ComponentDefinition
persistent identity: BBa J61146
display id: BBa_J61146
version: 1
was generated by: iGEM to SBOL conversion
description: {rbs1} Library Member in BBb
creator: John Anderson
created: 2007-09-03T11:00:00Z
modified: 2015-08-31T01:56:25Z
discontinued: false
dominant: false
experience: experience/None
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: {{BBbRBS1Family}}
mutable notes: {{BerkiGEM2007-Biobrick2.0Intro}}
mutable provenance: Synthetic
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J61146_sequence/1

BBa_I723012
type: ComponentDefinition
persistent identity: BBa I723012
display id: BBa_I723012
version: 1
was generated by: iGEM to SBOL conversion
description: Estimated RBS for DntR
creator: Scott Ramsay
created: 2007-10-24T11:00:00Z
modified: 2015-08-31T04:07:53Z
discontinued: false
dominant: false
experience: experience/None
group u list: _126_
m user id: 0
owner id: 2140
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is the naturally-occurring RBS found in the regulatory region of the DntR gene.
mutable notes: '''''This does not physically exist as a basic part due to time constraints; it was cloned as part of composite part which incorporates all of the components necessary for expression of a reporter gene under the control of DntR'''''. For this reason, the ends of the supplied sequence may not accurately represent the boundaries of the functional unit.
mutable provenance: Cloned from a historical plasmid based on pQF52 carrying genomic sequences from ''Burkholderia cepacia''.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: RBS for DntR
sequence: https://synbiohub.org/public/igem/BBa_I723012_sequence/1

RBS for DntR
type: SequenceAnnotation
persistent identity: annotation1954958
display id: annotation1954958
version: 1
top level: BBa_I723012
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_I723012/annotation1954958/range1954958/1

https://synbiohub.org/public/igem/BBa_I723012/annotation1954958/range1954958/1
type: Range
persistent identity: range1954958
display id: range1954958
version: 1
direction: direction/forward
top level: BBa_I723012
start: 1
end: 24
orientation: inline

BBa_J64013
type: ComponentDefinition
persistent identity: BBa J64013
display id: BBa_J64013
version: 1
was generated by: iGEM to SBOL conversion
description: sipA RBS
creator: Dan Widmaier
created: 2008-01-16T12:00:00Z
modified: 2015-08-31T01:56:26Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _98_
m user id: 0
owner id: 2407
owning group id: 98
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is the natural RBS that drives the sipA effector protein in the Salmonella SPI-1 system
mutable notes: This part was not designed with compatibility to biobrick standard assembly in mind. This RBS has not been explicitly tested in an expression assasy such as B-Gal or GFP expression.
mutable provenance: Salmonella LT2 Genomic DNA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: sicA RBS
sequence: https://synbiohub.org/public/igem/BBa_J64013_sequence/1

sicA RBS
type: SequenceAnnotation
persistent identity: annotation1959612
display id: annotation1959612
version: 1
top level: BBa_J64013
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_J64013/annotation1959612/range1959612/1

https://synbiohub.org/public/igem/BBa_J64013/annotation1959612/range1959612/1
type: Range
persistent identity: range1959612
display id: range1959612
version: 1
direction: direction/forward
top level: BBa_J64013
start: 1
end: 18
orientation: inline

BBa_B0077
type: ComponentDefinition
persistent identity: BBa B0077
display id: BBa_B0077
version: 1
was generated by: iGEM to SBOL conversion
description: Specialized RBS
creator: Bartholomew Canton
created: 2006-05-01T11:00:00Z
modified: 2015-08-31T04:07:21Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_6_
m user id: 0
owner id: 135
owning group id: 6
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_B0077_sequence/1

R38
type: ComponentDefinition
persistent identity: BBa K780001
display id: BBa_K780001
version: 1
was generated by: iGEM to SBOL conversion
description: Strong RBS for Bacillus Subtilis
creator: Krzysztof Szczepaniak
created: 2012-09-25T11:00:00Z
modified: 2015-05-08T01:13:18Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1034_
m user id: 0
owner id: 10271
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Natural RBS sequence for Bacillus Subtilis, described in the literature as a strong RBS for this bacteria.
mutable notes: -
mutable provenance: From genomic sequence Bacillus Subtilis.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_K780001_sequence/1

BBa_J61106
type: ComponentDefinition
persistent identity: BBa J61106
display id: BBa_J61106
version: 1
was generated by: iGEM to SBOL conversion
description: Ribosome Binding Site Family Member
creator: John Anderson
created: 2007-04-23T11:00:00Z
modified: 2015-08-31T02:03:00Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable description: {{JCA_Arkin_RBSFamily}}
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J61106_sequence/1

BBa_J61121
type: ComponentDefinition
persistent identity: BBa J61121
display id: BBa_J61121
version: 1
was generated by: iGEM to SBOL conversion
description: Ribosome Binding Site Family Member
creator: John Anderson
created: 2007-04-24T11:00:00Z
modified: 2015-08-31T01:59:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable description: {{JCA_Arkin_RBSFamily}}
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J61121_sequence/1

BBa_M13508
type: ComponentDefinition
persistent identity: BBa M13508
display id: BBa_M13508
version: 1
was generated by: iGEM to SBOL conversion
description: M13K07 gene VIII RBS
creator: Natalie Kuldell
created: 2006-12-14T12:00:00Z
modified: 2015-05-08T01:13:56Z
discontinued: false
dominant: true
experience: experience/None
group u list: _45_
m user id: 0
owner id: 314
owning group id: 1
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This RBS is part of the bacteriophage M13 genome, initiating translation of the gene VIII message (BBa_M13008). The part aligns with base pairs 1285-1300 in M13K07 genome. It was identified as the RBS for gene VIII message by Wezenbeek et al in Gene (1980) 11: 129-148
mutable notes: none
mutable provenance: New England Biolabs M13K07. Sequenced in 2002 by L. Panganaban
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_M13508_sequence/1

BBa_J61138
type: ComponentDefinition
persistent identity: BBa J61138
display id: BBa_J61138
version: 1
was generated by: iGEM to SBOL conversion
description: Ribosome Binding Site Family Member
creator: John Anderson
created: 2007-04-24T11:00:00Z
modified: 2015-08-31T01:59:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable description: {{JCA_Arkin_RBSFamily}}
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J61138_sequence/1

BBa_M13501
type: ComponentDefinition
persistent identity: BBa M13501
display id: BBa_M13501
version: 1
was generated by: iGEM to SBOL conversion
description: M13K07 gene I RBS
creator: Natalie Kuldell
created: 2006-12-14T12:00:00Z
modified: 2015-05-08T01:13:56Z
discontinued: false
dominant: false
experience: experience/None
group u list: _45_
m user id: 0
owner id: 314
owning group id: 1
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This RBS is part of the bacteriophage M13 genome, initiating translation of the gene I message (BBa_M13001). The part aligns with base pairs 8252-8267 in M13K07 genome. It was identified as the RBS for gene II message by Wezenbeek et al in Gene (1980) 11: 129-148
mutable notes: none
mutable provenance: New England Biolabs M13K07. Sequenced in 2002 by L. Panganaban
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_M13501_sequence/1

BBa_K1390004
type: ComponentDefinition
persistent identity: BBa K1390004
display id: BBa_K1390004
version: 1
was generated by: iGEM to SBOL conversion
description: mmoX
created: 2014-07-02T11:00:00Z
modified: 2015-05-08T01:10:14Z
mutable provenance: Amplified from the genomic DNA of Methylococcus capsulatus bath without any PstI restriction site
owned by: james
owned by: myers
mutable description: mmoX is one subunit of the mmo from Methylococcus capsulatus bath
discontinued: false
dominant: false
group u list: _1767_
m user id: 0
owner id: 17096
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: the brick is constructed in the RCF10 standard
star: false
creator: Stefan D??bel
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence: https://synbiohub.org/public/igem/BBa_K1390004_sequence/1

BBa_B0039
type: ComponentDefinition
persistent identity: BBa B0039
display id: BBa_B0039
version: 1
was generated by: iGEM to SBOL conversion
description: RBS 2
creator: Josh Michener
created: 2005-12-20T12:00:00Z
modified: 2015-08-31T04:07:20Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_
m user id: 0
owner id: 253
owning group id: 6
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Modified version of B0031 from the Voigt Lab. Not currently available as an independent part (only in a tet inverter).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_B0039_sequence/1

BBa_J23119
type: ComponentDefinition
persistent identity: BBa J23119
display id: BBa_J23119
version: 1
was generated by: iGEM to SBOL conversion
description: constitutive promoter family member
created: 2006-08-23T11:00:00Z
modified: 2015-08-31T04:08:40Z
mutable provenance: Overlap extension of synthetic oligonucleotides
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Later
discontinued: false
dominant: true
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable notes: N/A
star: true
creator: John Anderson
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J23119_sequence/1

BBa_J61140
type: ComponentDefinition
persistent identity: BBa J61140
display id: BBa_J61140
version: 1
was generated by: iGEM to SBOL conversion
description: {rbs1} Library Member in BBb
creator: John Anderson
created: 2007-09-03T11:00:00Z
modified: 2015-08-31T01:59:49Z
discontinued: false
dominant: false
experience: experience/None
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: {{BBbRBS1Family}}
mutable notes: {{BerkiGEM2007-Biobrick2.0Intro}}
mutable provenance: Synthetic
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J61140_sequence/1

Lock 1
type: ComponentDefinition
persistent identity: BBa J01010
display id: BBa_J01010
version: 1
was generated by: iGEM to SBOL conversion
description: Riboregulator Lock 1
creator: Golden Bear
created: 2005-11-05T12:00:00Z
modified: 2015-08-31T04:08:12Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _13_
m user id: 0
owner id: 395
owning group id: 13
sample status: In stock
status: status/Available
bookmark: false
mutable description: Biobricked version of Isaacs' riboregulator cis repressed lock, crR12.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000834
role: partType/RNA
sequence: https://synbiohub.org/public/igem/BBa_J01010_sequence/1

BBa_B2102
type: ComponentDefinition
persistent identity: BBa B2102
display id: BBa_B2102
version: 1
was generated by: iGEM to SBOL conversion
description: T7 RBS 0.4 + SapI (rev)
creator: Heather Keller
created: 2006-09-27T11:00:00Z
modified: 2015-08-31T04:07:21Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_
m user id: 0
owner id: 571
owning group id: 10
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Insert Text here
mutable notes: Insert Text here
mutable provenance: Insert Text here
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: BBa_B0079
sequence annotation: SD region
sequence annotation: BBa_B2002
sequence annotation: SapI
sequence: https://synbiohub.org/public/igem/BBa_B2102_sequence/1

BBa_B0079
type: SequenceAnnotation
persistent identity: annotation1902501
display id: annotation1902501
version: 1
top level: BBa_B2102
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_B2102/annotation1902501/range1902501/1

https://synbiohub.org/public/igem/BBa_B2102/annotation1902501/range1902501/1
type: Range
persistent identity: range1902501
display id: range1902501
version: 1
direction: direction/forward
top level: BBa_B2102
start: 21
end: 32
orientation: inline

SD region
type: SequenceAnnotation
persistent identity: annotation1902500
display id: annotation1902500
version: 1
top level: BBa_B2102
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_B2102/annotation1902500/range1902500/1

https://synbiohub.org/public/igem/BBa_B2102/annotation1902500/range1902500/1
type: Range
persistent identity: range1902500
display id: range1902500
version: 1
direction: direction/forward
top level: BBa_B2102
start: 4
end: 10
orientation: inline

BBa_B2002
type: SequenceAnnotation
persistent identity: annotation1902499
display id: annotation1902499
version: 1
top level: BBa_B2102
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_B2102/annotation1902499/range1902499/1

https://synbiohub.org/public/igem/BBa_B2102/annotation1902499/range1902499/1
type: Range
persistent identity: range1902499
display id: range1902499
version: 1
direction: direction/forward
top level: BBa_B2102
start: 1
end: 20
orientation: inline

SapI
type: SequenceAnnotation
persistent identity: annotation1902502
display id: annotation1902502
version: 1
top level: BBa_B2102
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_B2102/annotation1902502/range1902502/1

https://synbiohub.org/public/igem/BBa_B2102/annotation1902502/range1902502/1
type: Range
persistent identity: range1902502
display id: range1902502
version: 1
direction: direction/reverse
top level: BBa_B2102
start: 25
end: 31
orientation: inline

BBa_K103015
type: ComponentDefinition
persistent identity: BBa K103015
display id: BBa_K103015
version: 1
was generated by: iGEM to SBOL conversion
description: consensus RBS
creator: Michael Lower
created: 2008-10-08T11:00:00Z
modified: 2015-05-08T01:08:47Z
discontinued: false
dominant: false
experience: experience/None
group u list: _180_
m user id: 0
owner id: 2582
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: strong RBS
sequence: https://synbiohub.org/public/igem/BBa_K103015_sequence/1

strong RBS
type: SequenceAnnotation
persistent identity: annotation1979577
display id: annotation1979577
version: 1
top level: BBa_K103015
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K103015/annotation1979577/range1979577/1

https://synbiohub.org/public/igem/BBa_K103015/annotation1979577/range1979577/1
type: Range
persistent identity: range1979577
display id: range1979577
version: 1
direction: direction/forward
top level: BBa_K103015
start: 1
end: 6
orientation: inline

BBa_K1390006
type: ComponentDefinition
persistent identity: BBa K1390006
display id: BBa_K1390006
version: 1
was generated by: iGEM to SBOL conversion
description: mmoZ
created: 2014-07-02T11:00:00Z
modified: 2015-05-08T01:10:14Z
mutable provenance: Amplified from the genomic DNA of Methylococcus capsulatus bath
owned by: james
owned by: myers
mutable description: mmoZ is one subunit of the mmo from Methylococcus capsulatus bath
discontinued: false
dominant: false
group u list: _1767_
m user id: 0
owner id: 17096
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: the brick is constructed in the RCF10 standard
star: false
creator: Stefan D??bel
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence: https://synbiohub.org/public/igem/BBa_K1390006_sequence/1

BBa_J61114
type: ComponentDefinition
persistent identity: BBa J61114
display id: BBa_J61114
version: 1
was generated by: iGEM to SBOL conversion
description: Ribosome Binding Site Family Member
creator: John Anderson
created: 2007-04-23T11:00:00Z
modified: 2015-08-31T02:03:00Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable description: {{JCA_Arkin_RBSFamily}}
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J61114_sequence/1

BBa_J64011
type: ComponentDefinition
persistent identity: BBa J64011
display id: BBa_J64011
version: 1
was generated by: iGEM to SBOL conversion
description: invB RBS
creator: Dan Widmaier
created: 2008-01-15T12:00:00Z
modified: 2015-08-31T01:56:26Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _98_
m user id: 0
owner id: 2407
owning group id: 98
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: The natural RBS that drives invB
mutable notes: The expression level of this RBS has not been quantified.
mutable provenance: Salmonella LT2 Genomic DNA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: invB RBS
sequence: https://synbiohub.org/public/igem/BBa_J64011_sequence/1

invB RBS
type: SequenceAnnotation
persistent identity: annotation1959604
display id: annotation1959604
version: 1
top level: BBa_J64011
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_J64011/annotation1959604/range1959604/1

https://synbiohub.org/public/igem/BBa_J64011/annotation1959604/range1959604/1
type: Range
persistent identity: range1959604
display id: range1959604
version: 1
direction: direction/forward
top level: BBa_J64011
start: 1
end: 19
orientation: inline

BBa_K165002
type: ComponentDefinition
persistent identity: BBa K165002
display id: BBa_K165002
version: 1
was generated by: iGEM to SBOL conversion
description: Kozak sequence (yeast RBS)
creator: John Szymanski
created: 2008-10-25T11:00:00Z
modified: 2015-05-08T01:10:55Z
discontinued: false
dominant: false
experience: experience/None
group u list: _267_
m user id: 0
owner id: 2512
owning group id: 58
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: The kozak sequence acts as a eukaryotic RBS. It is cloned directly between a promoter and coding region.
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_K165002_sequence/1

BBa_K1390003
type: ComponentDefinition
persistent identity: BBa K1390003
display id: BBa_K1390003
version: 1
was generated by: iGEM to SBOL conversion
description: mmoD
created: 2014-07-02T11:00:00Z
modified: 2015-05-08T01:10:14Z
mutable provenance: Amplified from the genomic DNA of Methylococcus capsulatus bath
owned by: james
owned by: myers
mutable description: mmoD is one subunit of the mmo from Methylococcus capsulatus bath
discontinued: false
dominant: false
group u list: _1767_
m user id: 0
owner id: 17096
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: the brick is constructed in the RCF10 standard
star: false
creator: Stefan D??bel
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence: https://synbiohub.org/public/igem/BBa_K1390003_sequence/1

BBa_B0035
type: ComponentDefinition
persistent identity: BBa B0035
display id: BBa_B0035
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (B0030 derivative)
creator: Jason Kelly
created: 2004-01-27T12:00:00Z
modified: 2015-08-31T04:07:20Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_B0035_sequence/1

BBa_J64808
type: ComponentDefinition
persistent identity: BBa J64808
display id: BBa_J64808
version: 1
was generated by: iGEM to SBOL conversion
description: RocE RBS
creator: David Stanley
created: 2007-03-19T12:00:00Z
modified: 2015-05-08T01:08:17Z
discontinued: false
dominant: false
experience: experience/None
group u list: _98_
m user id: 0
owner id: 1423
owning group id: 98
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: RBS preceding RocE
mutable notes: n
mutable provenance: n
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J64808_sequence/1

BBa_J61124
type: ComponentDefinition
persistent identity: BBa J61124
display id: BBa_J61124
version: 1
was generated by: iGEM to SBOL conversion
description: Ribosome Binding Site Family Member
creator: John Anderson
created: 2007-04-24T11:00:00Z
modified: 2015-08-31T01:59:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable description: {{JCA_Arkin_RBSFamily}}
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J61124_sequence/1

BBa_J64026
type: ComponentDefinition
persistent identity: BBa J64026
display id: BBa_J64026
version: 1
was generated by: iGEM to SBOL conversion
description: sigE RBS
creator: Dan Widmaier
created: 2008-01-16T12:00:00Z
modified: 2015-08-31T01:56:26Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _98_
m user id: 0
owner id: 2407
owning group id: 98
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is the natural RBS for the sigE chaperone (BBa_J64025) in Salmonella Typhimurium LT2.
mutable notes: This part was not designed with compatibility to biobrick standard assembly in mind. This RBS has not been explicitly assayed for expression rate.
mutable provenance: Salmonella LT2 Genomic DNA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: sigE RBS
sequence: https://synbiohub.org/public/igem/BBa_J64026_sequence/1

sigE RBS
type: SequenceAnnotation
persistent identity: annotation1959625
display id: annotation1959625
version: 1
top level: BBa_J64026
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_J64026/annotation1959625/range1959625/1

https://synbiohub.org/public/igem/BBa_J64026/annotation1959625/range1959625/1
type: Range
persistent identity: range1959625
display id: range1959625
version: 1
direction: direction/forward
top level: BBa_J64026
start: 1
end: 18
orientation: inline

BBa_J61107
type: ComponentDefinition
persistent identity: BBa J61107
display id: BBa_J61107
version: 1
was generated by: iGEM to SBOL conversion
description: Ribosome Binding Site Family Member
creator: John Anderson
created: 2007-04-23T11:00:00Z
modified: 2015-08-31T02:03:00Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable description: {{JCA_Arkin_RBSFamily}}
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J61107_sequence/1

BBa_J61131
type: ComponentDefinition
persistent identity: BBa J61131
display id: BBa_J61131
version: 1
was generated by: iGEM to SBOL conversion
description: Ribosome Binding Site Family Member
creator: John Anderson
created: 2007-04-24T11:00:00Z
modified: 2015-08-31T01:59:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable description: {{JCA_Arkin_RBSFamily}}
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J61131_sequence/1

BBa_K090505
type: ComponentDefinition
persistent identity: BBa K090505
display id: BBa_K090505
version: 1
was generated by: iGEM to SBOL conversion
description: ''Bacillus subtilis'' consensus RBS
creator: Daniel Goodman
created: 2008-10-27T12:00:00Z
modified: 2015-05-08T01:08:37Z
discontinued: false
dominant: false
experience: experience/None
group u list: _188_
m user id: 0
owner id: 3501
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is the consensus RBS for Bacillus subtilis.
mutable notes: The part must be 8 nucleotides away from the first methionine of the coding sequence, and so we added 'TGT' downstream of the actual binding sequence: AAAGGAGG. This, combined with the truncated non-CDS BioBrick prefix scar, gives exactly 8 'spacer bases' between the 3' end of the RBS and the start of the coding sequence.
mutable provenance: This part was synthesized directly.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: B. subtilis consensus RBS
sequence: https://synbiohub.org/public/igem/BBa_K090505_sequence/1

B. subtilis consensus RBS
type: SequenceAnnotation
persistent identity: annotation1992802
display id: annotation1992802
version: 1
top level: BBa_K090505
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K090505/annotation1992802/range1992802/1

https://synbiohub.org/public/igem/BBa_K090505/annotation1992802/range1992802/1
type: Range
persistent identity: range1992802
display id: range1992802
version: 1
direction: direction/forward
top level: BBa_K090505
start: 1
end: 8
orientation: inline

BBa_B0078
type: ComponentDefinition
persistent identity: BBa B0078
display id: BBa_B0078
version: 1
was generated by: iGEM to SBOL conversion
description: Specialized RBS
creator: Bartholomew Canton
created: 2006-05-01T11:00:00Z
modified: 2015-08-31T04:07:21Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_6_
m user id: 0
owner id: 135
owning group id: 6
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_B0078_sequence/1

BBa_J61115
type: ComponentDefinition
persistent identity: BBa J61115
display id: BBa_J61115
version: 1
was generated by: iGEM to SBOL conversion
description: Ribosome Binding Site Family Member
creator: John Anderson
created: 2007-04-23T11:00:00Z
modified: 2015-08-31T02:03:00Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable description: {{JCA_Arkin_RBSFamily}}
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J61115_sequence/1

BBa_B0031
type: ComponentDefinition
persistent identity: BBa B0031
display id: BBa_B0031
version: 1
was generated by: iGEM to SBOL conversion
description: RBS.2 (weak) -- derivative of BBa_0030
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _41_44_48_46_1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Medium RBS based on Ron Weiss thesis. Strength considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("RBS-1" in figure 4-14 of thesis). No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Cho
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0031_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23316
display id: annotation23316
version: 1
top level: BBa_B0031
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_B0031/annotation23316/range23316/1

https://synbiohub.org/public/igem/BBa_B0031/annotation23316/range23316/1
type: Range
persistent identity: range23316
display id: range23316
version: 1
direction: direction/forward
top level: BBa_B0031
start: 7
end: 10
orientation: inline

BBa_J56016
type: ComponentDefinition
persistent identity: BBa J56016
display id: BBa_J56016
version: 1
was generated by: iGEM to SBOL conversion
description: Rbs-orig - ribosome binding site
creator: Matt Eames
created: 2006-10-31T12:00:00Z
modified: 2015-08-31T03:22:59Z
discontinued: false
dominant: false
experience: experience/None
group u list: _79_98_
m user id: 0
owner id: 1132
owning group id: 98
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Ribosome binding site for transcription initiation
mutable notes: None
mutable provenance: Synthesized by DNA 2.0
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J56016_sequence/1

BBa_K819017
type: ComponentDefinition
persistent identity: BBa K819017
display id: BBa_K819017
version: 1
was generated by: iGEM to SBOL conversion
description: Luminesensor repressible SulA408 promoter
creator: YU Zhou
created: 2012-09-15T11:00:00Z
modified: 2015-05-08T01:13:29Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1077_
m user id: 0
owner id: 13785
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: SulA promoter in 408 form.SulA promoter belongs to the SOS regulon family, and promotes in E.coli transcription of SulA protein. SulA408 promoter is reponsive to our optimized Luminesensor(BBa_K819005).
mutable notes: SulA promoter in 408 form.
mutable provenance: SulA promoter sequence is got from regulon.org.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: SulA408 Promoter
sequence: https://synbiohub.org/public/igem/BBa_K819017_sequence/1

SulA408 Promoter
type: SequenceAnnotation
persistent identity: annotation2197266
display id: annotation2197266
version: 1
top level: BBa_K819017
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K819017/annotation2197266/range2197266/1

https://synbiohub.org/public/igem/BBa_K819017/annotation2197266/range2197266/1
type: Range
persistent identity: range2197266
display id: range2197266
version: 1
direction: direction/forward
top level: BBa_K819017
start: 1
end: 81
orientation: inline

BBa_J64020
type: ComponentDefinition
persistent identity: BBa J64020
display id: BBa_J64020
version: 1
was generated by: iGEM to SBOL conversion
description: invI RBS
creator: Dan Widmaier
created: 2008-01-16T12:00:00Z
modified: 2015-08-31T01:56:26Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _98_
m user id: 0
owner id: 2407
owning group id: 98
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This part is the natural invI RBS from Salmonella Typhimurium LT2.
mutable notes: This part was not designed with consideration for biobrick standard assembly in mind. This RBS has not been explicitly tested for expression level in a fluorescence or B-Gal assay.
mutable provenance: Salmonella Typhimurium LT2 Genomic DNA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: invI RBS
sequence: https://synbiohub.org/public/igem/BBa_J64020_sequence/1

invI RBS
type: SequenceAnnotation
persistent identity: annotation1959619
display id: annotation1959619
version: 1
top level: BBa_J64020
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_J64020/annotation1959619/range1959619/1

https://synbiohub.org/public/igem/BBa_J64020/annotation1959619/range1959619/1
type: Range
persistent identity: range1959619
display id: range1959619
version: 1
direction: direction/forward
top level: BBa_J64020
start: 1
end: 20
orientation: inline

BBa_J61120
type: ComponentDefinition
persistent identity: BBa J61120
display id: BBa_J61120
version: 1
was generated by: iGEM to SBOL conversion
description: Ribosome Binding Site Family Member
creator: John Anderson
created: 2007-04-24T11:00:00Z
modified: 2015-08-31T01:59:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable description: {{JCA_Arkin_RBSFamily}}
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J61120_sequence/1

BBa_M13509
type: ComponentDefinition
persistent identity: BBa M13509
display id: BBa_M13509
version: 1
was generated by: iGEM to SBOL conversion
description: M13Ko7 gene IX RBS
creator: Natalie Kuldell
created: 2006-12-14T12:00:00Z
modified: 2015-05-08T01:13:56Z
discontinued: false
dominant: false
experience: experience/None
group u list: _45_
m user id: 0
owner id: 314
owning group id: 1
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This RBS is part of the bacteriophage M13 genome, initiating translation of the gene IX message (BBa_M13009). The part aligns with base pairs 1190-1205 in M13K07 genome. It was identified as the RBS for gene IX message by Wezenbeek et al in Gene (1980) 11: 129-148
mutable notes: none
mutable provenance: New England Biolabs M13K07. Sequenced in 2002 by L. Panganaban
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_M13509_sequence/1

BBa_B0064
type: ComponentDefinition
persistent identity: BBa B0064
display id: BBa_B0064
version: 1
was generated by: iGEM to SBOL conversion
description: Tuned RBS for Q04401
creator: Jason Kelly
created: 2008-02-23T12:00:00Z
modified: 2015-08-31T04:07:21Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_
m user id: 0
owner id: 2
owning group id: 84
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is a single bp change from B0034. It's strength is approximately 35% that of B0034.
mutable notes: .
mutable provenance: .
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_B0064_sequence/1

BBa_J61104
type: ComponentDefinition
persistent identity: BBa J61104
display id: BBa_J61104
version: 1
was generated by: iGEM to SBOL conversion
description: Ribosome Binding Site Family Member
creator: John Anderson
created: 2007-04-23T11:00:00Z
modified: 2015-08-31T02:03:00Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable description: {{JCA_Arkin_RBSFamily}}
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J61104_sequence/1

BBa_J61127
type: ComponentDefinition
persistent identity: BBa J61127
display id: BBa_J61127
version: 1
was generated by: iGEM to SBOL conversion
description: Ribosome Binding Site Family Member
creator: John Anderson
created: 2007-04-24T11:00:00Z
modified: 2015-08-31T01:59:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable description: {{JCA_Arkin_RBSFamily}}
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J61127_sequence/1

BBa_B0037
type: ComponentDefinition
persistent identity: BBa B0037
display id: BBa_B0037
version: 1
was generated by: iGEM to SBOL conversion
description: Specialized RBS
creator: Barry Canton
created: 2005-12-06T12:00:00Z
modified: 2015-08-31T04:07:20Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_6_
m user id: 0
owner id: 135
owning group id: 6
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: RBS specific to dedicated ribosomes. This RBS is a modified version of B0036. The spacing between the SD sequence and the start codon has been increased by 5 bases to give the same spacing as used by Brink et al. The 5 bases are the same as those used by Brink. That means they form the first five bases of an XbaI site and no negative affects of using these bases are known at present.
mutable provenance: Brink MF, Verbeet MP, de Boer HA (1995). Specialized ribosomes: highly specific translation in vivo of a single targetted mRNA species. Gene, 156, pp. 215-222
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_B0037_sequence/1

BBa_B0030
type: ComponentDefinition
persistent identity: BBa B0030
display id: BBa_B0030
version: 1
was generated by: iGEM to SBOL conversion
description: RBS.1 (strong) -- modified from R. Weiss
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _44_46_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("orig" in figure 4-14 of Ron Weiss thesis). No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info Brian Chow
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: BBa_B0030
sequence annotation: RBS-1\Strong
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_B0030_sequence/1

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation7025
display id: annotation7025
version: 1
top level: BBa_B0030
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_B0030/annotation7025/range7025/1

https://synbiohub.org/public/igem/BBa_B0030/annotation7025/range7025/1
type: Range
persistent identity: range7025
display id: range7025
version: 1
direction: direction/forward
top level: BBa_B0030
start: 1
end: 15
orientation: inline

RBS-1\Strong
type: SequenceAnnotation
persistent identity: annotation1701
display id: annotation1701
version: 1
top level: BBa_B0030
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_B0030/annotation1701/range1701/1

https://synbiohub.org/public/igem/BBa_B0030/annotation1701/range1701/1
type: Range
persistent identity: range1701
display id: range1701
version: 1
direction: direction/forward
top level: BBa_B0030
start: 1
end: 15
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1702
display id: annotation1702
version: 1
top level: BBa_B0030
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_B0030/annotation1702/range1702/1

https://synbiohub.org/public/igem/BBa_B0030/annotation1702/range1702/1
type: Range
persistent identity: range1702
display id: range1702
version: 1
direction: direction/forward
top level: BBa_B0030
start: 8
end: 12
orientation: inline

BBa_J61147
type: ComponentDefinition
persistent identity: BBa J61147
display id: BBa_J61147
version: 1
was generated by: iGEM to SBOL conversion
description: {rbs1} Library Member in BBb
creator: John Anderson
created: 2007-09-03T11:00:00Z
modified: 2015-08-31T01:56:25Z
discontinued: false
dominant: false
experience: experience/None
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: {{BBbRBS1Family}}
mutable notes: {{BerkiGEM2007-Biobrick2.0Intro}}
mutable provenance: Synthetic
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J61147_sequence/1

Kozak & st
type: ComponentDefinition
persistent identity: BBa J63003
display id: BBa_J63003
version: 1
was generated by: iGEM to SBOL conversion
description: designed yeast Kozak sequence
creator: Caroline Ajo-Franklin
created: 2006-10-10T11:00:00Z
modified: 2015-08-31T01:56:26Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _97_
m user id: 0
owner id: 545
owning group id: 97
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: consensus Kozak sequence and start codon
mutable notes: designed such that fusion using fusion bricks assembly method leads to in frame translation
mutable provenance: consensus Kozak and start built from oligonucleotides
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: start codon
sequence: https://synbiohub.org/public/igem/BBa_J63003_sequence/1

start codon
type: SequenceAnnotation
persistent identity: annotation1902852
display id: annotation1902852
version: 1
top level: Kozak & st
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_J63003/annotation1902852/range1902852/1

https://synbiohub.org/public/igem/BBa_J63003/annotation1902852/range1902852/1
type: Range
persistent identity: range1902852
display id: range1902852
version: 1
direction: direction/forward
top level: Kozak & st
start: 13
end: 15
orientation: inline

BBa_I11010
type: ComponentDefinition
persistent identity: BBa I11010
display id: BBa_I11010
version: 1
was generated by: iGEM to SBOL conversion
description: RBS
creator: bu
created: 2004-09-02T11:00:00Z
modified: 2015-08-31T04:07:30Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2_
m user id: 0
owner id: 102
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: ribosome binding site
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_I11010_sequence/1

RBS
type: SequenceAnnotation
persistent identity: annotation1147662
display id: annotation1147662
version: 1
top level: BBa_I11010
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_I11010/annotation1147662/range1147662/1

https://synbiohub.org/public/igem/BBa_I11010/annotation1147662/range1147662/1
type: Range
persistent identity: range1147662
display id: range1147662
version: 1
direction: direction/forward
top level: BBa_I11010
start: 1
end: 6
orientation: inline

BBa_J61117
type: ComponentDefinition
persistent identity: BBa J61117
display id: BBa_J61117
version: 1
was generated by: iGEM to SBOL conversion
description: Ribosome Binding Site Family Member
creator: John Anderson
created: 2007-04-23T11:00:00Z
modified: 2015-08-31T01:59:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable description: {{JCA_Arkin_RBSFamily}}
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J61117_sequence/1

BBa_J61122
type: ComponentDefinition
persistent identity: BBa J61122
display id: BBa_J61122
version: 1
was generated by: iGEM to SBOL conversion
description: Ribosome Binding Site Family Member
creator: John Anderson
created: 2007-04-24T11:00:00Z
modified: 2015-08-31T01:59:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: {{JCA_Arkin_RBSFamily}}
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J61122_sequence/1

BBa_J59001
type: ComponentDefinition
persistent identity: BBa J59001
display id: BBa_J59001
version: 1
was generated by: iGEM to SBOL conversion
description: tuba
creator: Fatos tuba
created: 2006-08-28T11:00:00Z
modified: 2015-08-31T02:02:59Z
discontinued: false
dominant: false
experience: experience/None
group u list: _93_
m user id: 0
owner id: 1162
owning group id: 93
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: none
mutable notes: none
mutable provenance: none
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: tuba
sequence: https://synbiohub.org/public/igem/BBa_J59001_sequence/1

tuba
type: SequenceAnnotation
persistent identity: annotation1898348
display id: annotation1898348
version: 1
top level: BBa_J59001
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_J59001/annotation1898348/range1898348/1

https://synbiohub.org/public/igem/BBa_J59001/annotation1898348/range1898348/1
type: Range
persistent identity: range1898348
display id: range1898348
version: 1
direction: direction/reverse
top level: BBa_J59001
start: 1
end: 25
orientation: inline

BBa_K1675001
type: ComponentDefinition
persistent identity: BBa K1675001
display id: BBa_K1675001
version: 1
was generated by: iGEM to SBOL conversion
description: NhaB; sodium-proton antiporter; membrane protein
created: 2015-09-08T11:00:00Z
modified: 2015-09-15T09:57:31Z
mutable provenance: Escherichia coli str. K-12 substr. Sequence found through Genbank http://www.ncbi.nlm.nih.gov/nuccore/NC_000913.3?report=fasta&from=1233176&to=1234717&strand=true
owned by: james
owned by: myers
mutable description: The gene, NhaB, is the archetypal sodium-proton antiporter in E. coli. It is crucial when the level of NhaA activity is growth limiting, when NhaA is not sufficiently induced, or when NhaA is not activated. NhaA catalyzes the exchange of 3 proton ion per 2 sodium ion. Therefore, NhaA could make sure E. coli???s survival under alkaline pH, which are in desperate need in our project.
discontinued: false
dominant: false
experience: experience/None
group u list: _2093_
m user id: 27740
owner id: 27740
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: mutation of CTG to CTA Leu.(site:336bp); mutation of GCT to GCG Ala.(site:414bp); mutation of ACC to ACT Thr.(site:519bp); mutation of GAC to GAT Asp.(site:525bp); mutation of ACC to ACT Thr.(site:630bp); mutation of GTA to GTG Val.(site:645bp); mutation of GCA to GCG Ala.(site:993bp); mutation of GAG to GAA Glu.(site:1020bp); mutation of GTC to GTA Val.(site:1452bp)
star: false
creator: JIAQI XU
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: NhaB Coding Region
sequence annotation: ATG start codon
sequence annotation: TGA stop codon
sequence: https://synbiohub.org/public/igem/BBa_K1675001_sequence/1

NhaB Coding Region
type: SequenceAnnotation
persistent identity: annotation2456965
display id: annotation2456965
version: 1
top level: BBa_K1675001
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1675001/annotation2456965/range2456965/1

https://synbiohub.org/public/igem/BBa_K1675001/annotation2456965/range2456965/1
type: Range
persistent identity: range2456965
display id: range2456965
version: 1
top level: BBa_K1675001
direction: direction/forward
start: 1
end: 1542
orientation: inline

ATG start codon
type: SequenceAnnotation
persistent identity: annotation2456966
display id: annotation2456966
version: 1
top level: BBa_K1675001
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1675001/annotation2456966/range2456966/1

https://synbiohub.org/public/igem/BBa_K1675001/annotation2456966/range2456966/1
type: Range
persistent identity: range2456966
display id: range2456966
version: 1
top level: BBa_K1675001
direction: direction/forward
start: 1
end: 3
orientation: inline

TGA stop codon
type: SequenceAnnotation
persistent identity: annotation2456967
display id: annotation2456967
version: 1
top level: BBa_K1675001
role: feature/stop
role: SO:0000319
location: https://synbiohub.org/public/igem/BBa_K1675001/annotation2456967/range2456967/1

https://synbiohub.org/public/igem/BBa_K1675001/annotation2456967/range2456967/1
type: Range
persistent identity: range2456967
display id: range2456967
version: 1
top level: BBa_K1675001
direction: direction/forward
start: 1540
end: 1542
orientation: inline

RBS-GsiB
type: ComponentDefinition
persistent identity: BBa K143020
display id: BBa_K143020
version: 1
was generated by: iGEM to SBOL conversion
description: GsiB ribosome binding site (RBS) for B. subtilis
creator: James Chappell
created: 2008-09-14T11:00:00Z
modified: 2015-05-08T01:10:23Z
discontinued: false
dominant: false
experience: experience/None
group u list: _199_
m user id: 0
owner id: 2090
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: GsiB is an endogenous ribosome binding site from ''B.subtilis''. The sequence of the gsiB ribosome binding site is '''AAAGGAGG''' which is complementary to the sequence '''UUUCCUCC''' from the 3' region of the 16s rRNA from ''B.subtilis''. GsiB is an endogenous ribosome binding site (RBS) from ''B.subtilis''. The sequence of the gsiB ribosome binding site is '''AAAGGAGG''' which is complementary to the sequence '''UUUCCUCC''' from the 3' region of the 16s rRNA from ''B.subtilis''. Previous research showed that the predicted binding energy of the 16s rRNA to the RBS is -9.3kcal.
mutable notes: In order to ensure that the RBS is functional the actual ribosome binding site was maintained and the distance between the RBS and the start codon maintained. In order to conform to the biobrick standard the sequence flanking the RBS had to be changed but the distance between the promoter and RBS, and start codon and RBS was maintained.
mutable provenance: The sequence was taken from a previous research paper [1] and was constructed by Geneart
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: rbs
sequence: https://synbiohub.org/public/igem/BBa_K143020_sequence/1

rbs
type: SequenceAnnotation
persistent identity: annotation1975872
display id: annotation1975872
version: 1
top level: RBS-GsiB
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K143020/annotation1975872/range1975872/1

https://synbiohub.org/public/igem/BBa_K143020/annotation1975872/range1975872/1
type: Range
persistent identity: range1975872
display id: range1975872
version: 1
direction: direction/forward
top level: RBS-GsiB
start: 2
end: 8
orientation: inline

creC RBS
type: ComponentDefinition
persistent identity: BBa J64908
display id: BBa_J64908
version: 1
was generated by: iGEM to SBOL conversion
description: RBS for creC in e coli operon
creator: Ryan Ritterson
created: 2007-03-19T12:00:00Z
modified: 2015-05-08T01:08:17Z
discontinued: false
dominant: false
experience: experience/None
group u list: _98_
m user id: 0
owner id: 1422
owning group id: 98
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: The ribosomal binding site for gene creC in the E Coli cre operon.
mutable notes: natural sequence, no design
mutable provenance: E Coli K12 strain.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J64908_sequence/1

BBa_J64809
type: ComponentDefinition
persistent identity: BBa J64809
display id: BBa_J64809
version: 1
was generated by: iGEM to SBOL conversion
description: RocF RBS
creator: David Stanley
created: 2007-03-19T12:00:00Z
modified: 2015-05-08T01:08:17Z
discontinued: false
dominant: false
experience: experience/None
group u list: _98_
m user id: 0
owner id: 1423
owning group id: 98
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: RBS upstream of RocF
mutable notes: n
mutable provenance: n
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J64809_sequence/1

BBa_J61144
type: ComponentDefinition
persistent identity: BBa J61144
display id: BBa_J61144
version: 1
was generated by: iGEM to SBOL conversion
description: {rbs1} Library Member in BBb
creator: John Anderson
created: 2007-09-03T11:00:00Z
modified: 2015-08-31T01:56:25Z
discontinued: false
dominant: false
experience: experience/None
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: {{BBbRBS1Family}}
mutable notes: {{BerkiGEM2007-Biobrick2.0Intro}}
mutable provenance: Synthetic
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J61144_sequence/1

BBa_J61112
type: ComponentDefinition
persistent identity: BBa J61112
display id: BBa_J61112
version: 1
was generated by: iGEM to SBOL conversion
description: Ribosome Binding Site Family Member
creator: John Anderson
created: 2007-04-23T11:00:00Z
modified: 2015-08-31T02:03:00Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable description: {{JCA_Arkin_RBSFamily}}
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J61112_sequence/1

BBa_K1899003
type: ComponentDefinition
persistent identity: BBa K1899003
display id: BBa_K1899003
version: 1
was generated by: iGEM to SBOL conversion
description: Superfolder GFP
creator: iGEM2016_HKUST
created: 2016-08-27T11:00:00Z
modified: 2016-10-15T04:03:09Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2365_
m user id: 26069
owner id: 26069
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Variant of GFP that folds robustly even when fused to poorly folded proteins. Waldo and other research groups has reported the engineering of a superfolder GFP (sfGFP) that showed increased resistance to denaturation, improved folding kinetics, and increased resistance to aggregation during refolding (Pedelacq et al., 2006; Andrews et al., 2007; Fisher and DeLisa, 2008). sfGFP has proven to be very useful as a scaffold for improved protein detection and tagging both in vivo and in vitro using self-assembled sfGFP fragments (Cabantous et al., 2005b; Cabantous and Waldo, 2006). Furthermore, sfGFP fusions are more soluble than conventional GFP fusions (Wu et al., 2009).
mutable notes: nil
mutable provenance: Waldo lab
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: BBa_J61048
sequence annotation: sfGFP
sequence annotation: scar
sequence annotation: BBa_B0032
sequence: https://synbiohub.org/public/igem/BBa_K1899003_sequence/1

BBa_J61048
type: SequenceAnnotation
persistent identity: annotation2481805
display id: annotation2481805
version: 1
top level: BBa_K1899003
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1899003/annotation2481805/range2481805/1

https://synbiohub.org/public/igem/BBa_K1899003/annotation2481805/range2481805/1
type: Range
persistent identity: range2481805
display id: range2481805
version: 1
direction: direction/forward
top level: BBa_K1899003
start: 742
end: 854
orientation: inline

sfGFP
type: SequenceAnnotation
persistent identity: annotation2481803
display id: annotation2481803
version: 1
top level: BBa_K1899003
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1899003/annotation2481803/range2481803/1

https://synbiohub.org/public/igem/BBa_K1899003/annotation2481803/range2481803/1
type: Range
persistent identity: range2481803
display id: range2481803
version: 1
direction: direction/forward
top level: BBa_K1899003
start: 20
end: 733
orientation: inline

scar
type: SequenceAnnotation
persistent identity: annotation2481804
display id: annotation2481804
version: 1
top level: BBa_K1899003
role: feature/scar
location: https://synbiohub.org/public/igem/BBa_K1899003/annotation2481804/range2481804/1

https://synbiohub.org/public/igem/BBa_K1899003/annotation2481804/range2481804/1
type: Range
persistent identity: range2481804
display id: range2481804
version: 1
direction: direction/forward
top level: BBa_K1899003
start: 13
end: 19
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation2481802
display id: annotation2481802
version: 1
top level: BBa_K1899003
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1899003/annotation2481802/range2481802/1

https://synbiohub.org/public/igem/BBa_K1899003/annotation2481802/range2481802/1
type: Range
persistent identity: range2481802
display id: range2481802
version: 1
direction: direction/forward
top level: BBa_K1899003
start: 1
end: 12
orientation: inline

scar
type: SequenceAnnotation
persistent identity: annotation2481806
display id: annotation2481806
version: 1
top level: BBa_K1899003
role: feature/scar
location: https://synbiohub.org/public/igem/BBa_K1899003/annotation2481806/range2481806/1

https://synbiohub.org/public/igem/BBa_K1899003/annotation2481806/range2481806/1
type: Range
persistent identity: range2481806
display id: range2481806
version: 1
direction: direction/forward
top level: BBa_K1899003
start: 734
end: 741
orientation: inline

lacI+pL
type: ComponentDefinition
persistent identity: BBa R0011
display id: BBa_R0011
version: 1
was generated by: iGEM to SBOL conversion
description: Promoter (lacI regulated, lambda pL hybrid)
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: represillator of Elowitz and Leibler (2000)
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) The PLlac 0-1 promoter is a hybrid regulatory region consisting of the promoter P(L) of phage lambda with the cI binding sites replaced with lacO1. The hybrid design allows for strong promotion that can nevertheless be tightly repressed by LacI, the Lac inhibitor (i.e. repressor) (<bb_part>BBa_C0010</bb_part>) ([LUTZ97]). The activity of the promoter can be regulated over a >600-fold range by IPTG in E.Coli DH5-alpha-Z1 (same paper reference).
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: hybrid promoter design to create strong promoter that is, at the same time, highly repressible. note that the upstream operator installed in this hybrid is slightly different than the one in the original source (Lutz and Bujard, 1997). the most upstream operator region is slightly truncated in the represillator version, so that both operators in the hybrid are the same sequence. see references for details. also, the sequence has been truncated after the transcriptional start site. LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and increase transcription. This part is incompatible with environments containing lactose or lactose analogs.
star: true
creator: Neelaksh Varshney, Grace Kenney, Daniel Shen, Samantha Sutton
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -10
sequence annotation: -35
sequence annotation: lac O1
sequence annotation: BBa_R0011
sequence: https://synbiohub.org/public/igem/BBa_R0011_sequence/1

-10
type: SequenceAnnotation
persistent identity: annotation2002
display id: annotation2002
version: 1
top level: lacI+pL
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0011/annotation2002/range2002/1

https://synbiohub.org/public/igem/BBa_R0011/annotation2002/range2002/1
type: Range
persistent identity: range2002
display id: range2002
version: 1
top level: lacI+pL
direction: direction/forward
start: 43
end: 48
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2000
display id: annotation2000
version: 1
top level: lacI+pL
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0011/annotation2000/range2000/1

https://synbiohub.org/public/igem/BBa_R0011/annotation2000/range2000/1
type: Range
persistent identity: range2000
display id: range2000
version: 1
top level: lacI+pL
direction: direction/forward
start: 20
end: 25
orientation: inline

lac O1
type: SequenceAnnotation
persistent identity: annotation2001
display id: annotation2001
version: 1
top level: lacI+pL
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_R0011/annotation2001/range2001/1

https://synbiohub.org/public/igem/BBa_R0011/annotation2001/range2001/1
type: Range
persistent identity: range2001
display id: range2001
version: 1
top level: lacI+pL
direction: direction/forward
start: 26
end: 42
orientation: inline

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation7064
display id: annotation7064
version: 1
top level: lacI+pL
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_R0011/annotation7064/range7064/1

https://synbiohub.org/public/igem/BBa_R0011/annotation7064/range7064/1
type: Range
persistent identity: range7064
display id: range7064
version: 1
top level: lacI+pL
direction: direction/forward
start: 1
end: 54
orientation: inline

lac O1
type: SequenceAnnotation
persistent identity: annotation1999
display id: annotation1999
version: 1
top level: lacI+pL
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_R0011/annotation1999/range1999/1

https://synbiohub.org/public/igem/BBa_R0011/annotation1999/range1999/1
type: Range
persistent identity: range1999
display id: range1999
version: 1
top level: lacI+pL
direction: direction/forward
start: 3
end: 19
orientation: inline

revRBS
type: ComponentDefinition
persistent identity: BBa I742130
display id: BBa_I742130
version: 1
was generated by: iGEM to SBOL conversion
description: Reverse RBS
creator: Caroline Dahl
created: 2007-10-21T11:00:00Z
modified: 2015-08-31T04:08:03Z
discontinued: false
dominant: false
experience: experience/None
group u list: _123_
m user id: 0
owner id: 1968
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: DNA is double-stranded. To translate genes of both strands, you need RBSs in both strands.
mutable notes: Please refer to the original (BB_B0034).
mutable provenance: Please refer to the original (BB_B0034).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_I742130_sequence/1

R37
type: ComponentDefinition
persistent identity: BBa K780002
display id: BBa_K780002
version: 1
was generated by: iGEM to SBOL conversion
description: Strong RBS for Bacillus Subtilis
creator: Krzysztof Szczepaniak
created: 2012-09-25T11:00:00Z
modified: 2015-05-08T01:13:18Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1034_
m user id: 0
owner id: 10113
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Strong RBS for Bacillus Subtilis
mutable notes: -
mutable provenance: 'host-takover module' from B.subtilis Bacteriophage SPO1
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_K780002_sequence/1

BBa_M13505
type: ComponentDefinition
persistent identity: BBa M13505
display id: BBa_M13505
version: 1
was generated by: iGEM to SBOL conversion
description: M13KO7 gene V RBS
creator: Natalie Kuldell
created: 2006-12-14T12:00:00Z
modified: 2015-05-08T01:13:56Z
discontinued: false
dominant: false
experience: experience/None
group u list: _45_
m user id: 0
owner id: 314
owning group id: 1
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This RBS is part of the bacteriophage M13 genome, initiating translation of the gene V message (BBa_M13005). The part aligns with base pairs 827-842 in M13K07 genome. It was identified as the RBS for the gene V message by Wezenbeek et al in Gene (1980) 11: 129-148
mutable notes: none
mutable provenance: New England Biolabs M13K07. Sequenced in 2002 by L. Panganaban
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_M13505_sequence/1

BBa_M13506
type: ComponentDefinition
persistent identity: BBa M13506
display id: BBa_M13506
version: 1
was generated by: iGEM to SBOL conversion
description: M13K07 gene VI RBS
creator: Natalie Kuldell
created: 2006-12-14T12:00:00Z
modified: 2015-05-08T01:13:56Z
discontinued: false
dominant: false
experience: experience/None
group u list: _45_
m user id: 0
owner id: 314
owning group id: 1
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This RBS is part of the bacteriophage M13 genome, initiating translation of the gene VI message (BBa_M13006). The part aligns with base pairs 2840-2855 in M13K07 genome. It was identified as the RBS for gene VI message by Wezenbeek et al in Gene (1980) 11: 129-148
mutable notes: none
mutable provenance: New England Biolabs M13K07. Sequenced in 2002 by L. Panganaban
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_M13506_sequence/1

BBa_B0071
type: ComponentDefinition
persistent identity: BBa B0071
display id: BBa_B0071
version: 1
was generated by: iGEM to SBOL conversion
description: Specialized RBS
creator: Barry Canton
created: 2006-03-03T12:00:00Z
modified: 2015-08-31T04:07:21Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_6_
m user id: 0
owner id: 135
owning group id: 6
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This RBS has the modified Shine-Dalgarno sequence, it has a reduced secondary structure relative to using the actual Brink sequence with mixed connective sites flanking it. This RBS is modified from B0071 by reducing the 3' sequence so that the SD to ATG spacing is closer to that which is optimal for E. coli.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_B0071_sequence/1

nickname
type: ComponentDefinition
persistent identity: BBa K1479004
display id: BBa_K1479004
version: 1
was generated by: iGEM to SBOL conversion
description: Just for upload test
creator: ChenYB
created: 2014-09-30T11:00:00Z
modified: 2015-05-08T01:10:40Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1859_
m user id: 0
owner id: 21515
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This part is just for upload test. It is meaningless
mutable notes: NULL
mutable provenance: NULL
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: misc
sequence annotation: PolyA
sequence annotation: polya
sequence annotation: stem_loop
sequence annotation: BBa_B0012
sequence annotation: BBa_B1008
sequence annotation: stop
sequence annotation: BBa_B0011
sequence annotation: T7 TE
sequence annotation: BBa_B0014
sequence annotation: 22
sequence annotation: BBa_B0015
sequence annotation: BBa_B1007
sequence annotation: BBa_K1479001
sequence annotation: modified thr terminator
sequence annotation: BBa_K1479002
sequence annotation: BBa_B0010
sequence annotation: modified thr
sequence annotation: B1007
sequence annotation: B1008
sequence: https://synbiohub.org/public/igem/BBa_K1479004_sequence/1

misc
type: SequenceAnnotation
persistent identity: annotation2397990
display id: annotation2397990
version: 1
top level: nickname
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1479004/annotation2397990/range2397990/1

https://synbiohub.org/public/igem/BBa_K1479004/annotation2397990/range2397990/1
type: Range
persistent identity: range2397990
display id: range2397990
version: 1
direction: direction/reverse
top level: nickname
start: 1
end: 20
orientation: inline

PolyA
type: SequenceAnnotation
persistent identity: annotation2397970
display id: annotation2397970
version: 1
top level: nickname
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_K1479004/annotation2397970/range2397970/1

https://synbiohub.org/public/igem/BBa_K1479004/annotation2397970/range2397970/1
type: Range
persistent identity: range2397970
display id: range2397970
version: 1
direction: direction/forward
top level: nickname
start: 135
end: 139
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation2397988
display id: annotation2397988
version: 1
top level: nickname
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_K1479004/annotation2397988/range2397988/1

https://synbiohub.org/public/igem/BBa_K1479004/annotation2397988/range2397988/1
type: Range
persistent identity: range2397988
display id: range2397988
version: 1
direction: direction/forward
top level: nickname
start: 418
end: 431
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation2397964
display id: annotation2397964
version: 1
top level: nickname
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1479004/annotation2397964/range2397964/1

https://synbiohub.org/public/igem/BBa_K1479004/annotation2397964/range2397964/1
type: Range
persistent identity: range2397964
display id: range2397964
version: 1
direction: direction/forward
top level: nickname
start: 62
end: 84
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2397959
display id: annotation2397959
version: 1
top level: nickname
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1479004/annotation2397959/range2397959/1

https://synbiohub.org/public/igem/BBa_K1479004/annotation2397959/range2397959/1
type: Range
persistent identity: range2397959
display id: range2397959
version: 1
direction: direction/forward
top level: nickname
start: 1
end: 41
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2397976
display id: annotation2397976
version: 1
top level: nickname
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1479004/annotation2397976/range2397976/1

https://synbiohub.org/public/igem/BBa_K1479004/annotation2397976/range2397976/1
type: Range
persistent identity: range2397976
display id: range2397976
version: 1
direction: direction/forward
top level: nickname
start: 200
end: 240
orientation: inline

PolyA
type: SequenceAnnotation
persistent identity: annotation2397968
display id: annotation2397968
version: 1
top level: nickname
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_K1479004/annotation2397968/range2397968/1

https://synbiohub.org/public/igem/BBa_K1479004/annotation2397968/range2397968/1
type: Range
persistent identity: range2397968
display id: range2397968
version: 1
direction: direction/reverse
top level: nickname
start: 108
end: 112
orientation: inline

BBa_B1008
type: SequenceAnnotation
persistent identity: annotation2397967
display id: annotation2397967
version: 1
top level: nickname
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1479004/annotation2397967/range2397967/1

https://synbiohub.org/public/igem/BBa_K1479004/annotation2397967/range2397967/1
type: Range
persistent identity: range2397967
display id: range2397967
version: 1
direction: direction/reverse
top level: nickname
start: 104
end: 143
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2397989
display id: annotation2397989
version: 1
top level: nickname
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1479004/annotation2397989/range2397989/1

https://synbiohub.org/public/igem/BBa_K1479004/annotation2397989/range2397989/1
type: Range
persistent identity: range2397989
display id: range2397989
version: 1
direction: direction/forward
top level: nickname
start: 424
end: 424
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation2397982
display id: annotation2397982
version: 1
top level: nickname
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1479004/annotation2397982/range2397982/1

https://synbiohub.org/public/igem/BBa_K1479004/annotation2397982/range2397982/1
type: Range
persistent identity: range2397982
display id: range2397982
version: 1
direction: direction/forward
top level: nickname
start: 261
end: 283
orientation: inline

BBa_B0011
type: SequenceAnnotation
persistent identity: annotation2397963
display id: annotation2397963
version: 1
top level: nickname
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1479004/annotation2397963/range2397963/1

https://synbiohub.org/public/igem/BBa_K1479004/annotation2397963/range2397963/1
type: Range
persistent identity: range2397963
display id: range2397963
version: 1
direction: direction/forward
top level: nickname
start: 50
end: 95
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation2397978
display id: annotation2397978
version: 1
top level: nickname
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1479004/annotation2397978/range2397978/1

https://synbiohub.org/public/igem/BBa_K1479004/annotation2397978/range2397978/1
type: Range
persistent identity: range2397978
display id: range2397978
version: 1
direction: direction/forward
top level: nickname
start: 207
end: 226
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2397980
display id: annotation2397980
version: 1
top level: nickname
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1479004/annotation2397980/range2397980/1

https://synbiohub.org/public/igem/BBa_K1479004/annotation2397980/range2397980/1
type: Range
persistent identity: range2397980
display id: range2397980
version: 1
direction: direction/forward
top level: nickname
start: 233
end: 233
orientation: inline

BBa_B0014
type: SequenceAnnotation
persistent identity: annotation2397977
display id: annotation2397977
version: 1
top level: nickname
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1479004/annotation2397977/range2397977/1

https://synbiohub.org/public/igem/BBa_K1479004/annotation2397977/range2397977/1
type: Range
persistent identity: range2397977
display id: range2397977
version: 1
direction: direction/reverse
top level: nickname
start: 200
end: 294
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation2397961
display id: annotation2397961
version: 1
top level: nickname
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_K1479004/annotation2397961/range2397961/1

https://synbiohub.org/public/igem/BBa_K1479004/annotation2397961/range2397961/1
type: Range
persistent identity: range2397961
display id: range2397961
version: 1
direction: direction/forward
top level: nickname
start: 28
end: 41
orientation: inline

22
type: SequenceAnnotation
persistent identity: annotation2397957
display id: annotation2397957
version: 1
top level: nickname
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1479004/annotation2397957/range2397957/1

https://synbiohub.org/public/igem/BBa_K1479004/annotation2397957/range2397957/1
type: Range
persistent identity: range2397957
display id: range2397957
version: 1
direction: direction/forward
top level: nickname
start: 1
end: 10
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2397984
display id: annotation2397984
version: 1
top level: nickname
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1479004/annotation2397984/range2397984/1

https://synbiohub.org/public/igem/BBa_K1479004/annotation2397984/range2397984/1
type: Range
persistent identity: range2397984
display id: range2397984
version: 1
direction: direction/reverse
top level: nickname
start: 303
end: 431
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation2397985
display id: annotation2397985
version: 1
top level: nickname
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1479004/annotation2397985/range2397985/1

https://synbiohub.org/public/igem/BBa_K1479004/annotation2397985/range2397985/1
type: Range
persistent identity: range2397985
display id: range2397985
version: 1
direction: direction/forward
top level: nickname
start: 314
end: 357
orientation: inline

BBa_B0011
type: SequenceAnnotation
persistent identity: annotation2397981
display id: annotation2397981
version: 1
top level: nickname
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1479004/annotation2397981/range2397981/1

https://synbiohub.org/public/igem/BBa_K1479004/annotation2397981/range2397981/1
type: Range
persistent identity: range2397981
display id: range2397981
version: 1
direction: direction/forward
top level: nickname
start: 249
end: 294
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2397962
display id: annotation2397962
version: 1
top level: nickname
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1479004/annotation2397962/range2397962/1

https://synbiohub.org/public/igem/BBa_K1479004/annotation2397962/range2397962/1
type: Range
persistent identity: range2397962
display id: range2397962
version: 1
direction: direction/forward
top level: nickname
start: 34
end: 34
orientation: inline

misc
type: SequenceAnnotation
persistent identity: annotation2397956
display id: annotation2397956
version: 1
top level: nickname
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1479004/annotation2397956/range2397956/1

https://synbiohub.org/public/igem/BBa_K1479004/annotation2397956/range2397956/1
type: Range
persistent identity: range2397956
display id: range2397956
version: 1
direction: direction/forward
top level: nickname
start: 1
end: 20
orientation: inline

BBa_B1007
type: SequenceAnnotation
persistent identity: annotation2397971
display id: annotation2397971
version: 1
top level: nickname
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1479004/annotation2397971/range2397971/1

https://synbiohub.org/public/igem/BBa_K1479004/annotation2397971/range2397971/1
type: Range
persistent identity: range2397971
display id: range2397971
version: 1
direction: direction/reverse
top level: nickname
start: 152
end: 191
orientation: inline

BBa_K1479001
type: SequenceAnnotation
persistent identity: annotation2397965
display id: annotation2397965
version: 1
top level: nickname
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1479004/annotation2397965/range2397965/1

https://synbiohub.org/public/igem/BBa_K1479004/annotation2397965/range2397965/1
type: Range
persistent identity: range2397965
display id: range2397965
version: 1
direction: direction/reverse
top level: nickname
start: 104
end: 431
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation2397979
display id: annotation2397979
version: 1
top level: nickname
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_K1479004/annotation2397979/range2397979/1

https://synbiohub.org/public/igem/BBa_K1479004/annotation2397979/range2397979/1
type: Range
persistent identity: range2397979
display id: range2397979
version: 1
direction: direction/forward
top level: nickname
start: 227
end: 240
orientation: inline

modified thr terminator
type: SequenceAnnotation
persistent identity: annotation2397969
display id: annotation2397969
version: 1
top level: nickname
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1479004/annotation2397969/range2397969/1

https://synbiohub.org/public/igem/BBa_K1479004/annotation2397969/range2397969/1
type: Range
persistent identity: range2397969
display id: range2397969
version: 1
direction: direction/forward
top level: nickname
start: 113
end: 134
orientation: inline

BBa_K1479002
type: SequenceAnnotation
persistent identity: annotation2397955
display id: annotation2397955
version: 1
top level: nickname
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1479004/annotation2397955/range2397955/1

https://synbiohub.org/public/igem/BBa_K1479004/annotation2397955/range2397955/1
type: Range
persistent identity: range2397955
display id: range2397955
version: 1
direction: direction/reverse
top level: nickname
start: 1
end: 95
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2397983
display id: annotation2397983
version: 1
top level: nickname
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1479004/annotation2397983/range2397983/1

https://synbiohub.org/public/igem/BBa_K1479004/annotation2397983/range2397983/1
type: Range
persistent identity: range2397983
display id: range2397983
version: 1
direction: direction/forward
top level: nickname
start: 303
end: 382
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation2397987
display id: annotation2397987
version: 1
top level: nickname
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1479004/annotation2397987/range2397987/1

https://synbiohub.org/public/igem/BBa_K1479004/annotation2397987/range2397987/1
type: Range
persistent identity: range2397987
display id: range2397987
version: 1
direction: direction/forward
top level: nickname
start: 398
end: 417
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2397986
display id: annotation2397986
version: 1
top level: nickname
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1479004/annotation2397986/range2397986/1

https://synbiohub.org/public/igem/BBa_K1479004/annotation2397986/range2397986/1
type: Range
persistent identity: range2397986
display id: range2397986
version: 1
direction: direction/forward
top level: nickname
start: 391
end: 431
orientation: inline

modified thr
type: SequenceAnnotation
persistent identity: annotation2397974
display id: annotation2397974
version: 1
top level: nickname
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1479004/annotation2397974/range2397974/1

https://synbiohub.org/public/igem/BBa_K1479004/annotation2397974/range2397974/1
type: Range
persistent identity: range2397974
display id: range2397974
version: 1
direction: direction/forward
top level: nickname
start: 161
end: 182
orientation: inline

PolyA
type: SequenceAnnotation
persistent identity: annotation2397975
display id: annotation2397975
version: 1
top level: nickname
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_K1479004/annotation2397975/range2397975/1

https://synbiohub.org/public/igem/BBa_K1479004/annotation2397975/range2397975/1
type: Range
persistent identity: range2397975
display id: range2397975
version: 1
direction: direction/forward
top level: nickname
start: 183
end: 188
orientation: inline

B1007
type: SequenceAnnotation
persistent identity: annotation2397972
display id: annotation2397972
version: 1
top level: nickname
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1479004/annotation2397972/range2397972/1

https://synbiohub.org/public/igem/BBa_K1479004/annotation2397972/range2397972/1
type: Range
persistent identity: range2397972
display id: range2397972
version: 1
direction: direction/forward
top level: nickname
start: 152
end: 191
orientation: inline

PolyA
type: SequenceAnnotation
persistent identity: annotation2397973
display id: annotation2397973
version: 1
top level: nickname
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_K1479004/annotation2397973/range2397973/1

https://synbiohub.org/public/igem/BBa_K1479004/annotation2397973/range2397973/1
type: Range
persistent identity: range2397973
display id: range2397973
version: 1
direction: direction/reverse
top level: nickname
start: 155
end: 160
orientation: inline

B1008
type: SequenceAnnotation
persistent identity: annotation2397966
display id: annotation2397966
version: 1
top level: nickname
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1479004/annotation2397966/range2397966/1

https://synbiohub.org/public/igem/BBa_K1479004/annotation2397966/range2397966/1
type: Range
persistent identity: range2397966
display id: range2397966
version: 1
direction: direction/forward
top level: nickname
start: 104
end: 143
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation2397960
display id: annotation2397960
version: 1
top level: nickname
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1479004/annotation2397960/range2397960/1

https://synbiohub.org/public/igem/BBa_K1479004/annotation2397960/range2397960/1
type: Range
persistent identity: range2397960
display id: range2397960
version: 1
direction: direction/forward
top level: nickname
start: 8
end: 27
orientation: inline

https://synbiohub.org/public/igem/BBa_K1830001_sequence/1
type: Sequence
persistent identity: BBa K1830001 sequence
display id: BBa_K1830001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gaaacattaacaaatctaaaacagtcttaattctatcttgagaaagtattggtaataatattattgtcgataacgcgagcataataaacggctctgattaaattctgaagtttgttagatacaatgatttcgttcgaaggaactacaaaataaattataaggaggcactcaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1675000_sequence/1
type: Sequence
persistent identity: BBa K1675000 sequence
display id: BBa_K1675000_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gtgaaacatctgcatcgattctttagcagtgatgcctcgggaggcattattcttatcattgccgctatcctggcgatgattatggccaacagcggcgcaaccagtggatggtatcacgactttctggagacgccggttcagctccgggttggttcactcgaaatcaacaaaaacatgctgttatggataaatgacgcgctgatggcggtatttttcctgttagtcggtctggaagttaaacgtgaactgatgcaaggatcgctagccagcttacgccaggccgcatttccagttatcgccgctattggtgggatgattgtgccggcattactctatctggcttttaactatgccgatccgattacccgcgaagggtgggcgatcccggcggctactgacattgcttttgcacttggtgtactggcgctgttgggaagtcgtgttccgttagcgctgaagatctttttgatggctctggctattatcgacgatcttggggccatcattatcatcgcattgttctacactaatgacttatcgatggcctctcttggcgtcgcggctgtagcaattgcggtactcgcggtattgaatctgtgtggtgcacgccgcacgggcgtctatattcttgttggcgtggtgttgtggactgcggtgttgaaatcgggggttcacgcaactctggcgggggtaattgtcggcttctttattcctttgaaagagaagcatgggcgttctccagcgaagcgactggagcatgtgttgcacccgtgggtggcgtatctgattttgccgctgtttgcatttgctaatgctggcgtttcactgcaaggcgtcacgctggatggcttgacctccattctgccattggggatcatcgctggcttgctgattggcaaaccgctggggattagtctgttctgctggttggcgctgcgtttgaaactggcgcatctgcctgagggaacgacttatcagcaaattatggtggtggggatcctgtgcggtatcggttttactatgtctatctttattgccagcctggcctttggtagcgtagatccagaactgattaactgggcgaaactcggtatcctggtcggttctatctcttcggcggtaattggatacagctggttacgcgttcgtttgcgtccatcagtttga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1390003_sequence/1
type: Sequence
persistent identity: BBa K1390003 sequence
display id: BBa_K1390003_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggtcgaatcggcatttcagccattttcgggcgacgcagacgaatggttcgaggaaccacggccccaggccggtttcttcccttccgcggactggcatctgctcaaacgggacgagacctacgcagcctatgccaaggatctcgatttcatgtggcggtgggtcatcgtccgggaagaaaggatcgtccaggagggttgctcgatcagcctggagtcgtcgatccgcgccgtgacgcacgtactgaattattttggtatgaccgaacaacgcgccccggcagaggaccggaccggcggagttcaacattga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1390002_sequence/1
type: Sequence
persistent identity: BBa K1390002 sequence
display id: BBa_K1390002_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgcagcgagttcacactatcacggcggtgacggaggatggcgaatcgctccgcttcgaatgccgttcggacgaggacgtcatcaccgccgccctgcgccagaacatctttctgatgtcgtcctgccgggagggcggctgtgcgacctgcaaggccttgtgcagcgaaggggactacgacctcaagggctgtagcgttcaggcgctgccgccggaagaggaggaggaagggttggtgttgttgtgccggacctacccgaagaccgacctggaaatcgaactgccctatacccattgccgcatcagttttggtgaggtcggcagtttcgaggcggaggtcgtcggcctcaactgggtttcgagcaacaccgtccagtttcttttgcagaagcggcccgacgagtgcggcaaccgtggcgtgaaattcgaacccggtcagttcatggacctgaccatccccggcaccgatgtctcccgctcctactcgccggcgaaccttcctaatcccgaaggccgcctggagttcctgatccgcgtgttaccggagggacggttttcggactacctgcgcaatgacgcgcgtgtcggacaggtcctctcggtcaaagggccactgggcgtgttcggtctcaaggagcggggcatggcgccgcgctatttcgtggccggcggcaccgggttggcgccggtggtctcgatggtgcggcagatgcaggagtggaccgcgccgaacgagacccgcatctatttcggtgtgaacaccgagccggaattgttctacatcgacgagctcaaatccctggaacgatcgatgcgcaatctcaccgtgaaggcctgtgtctggcacccgagcggggactgggaaggcgagcagggctcgcccatcgatgcgttgcgggaagacctggagtcctccgacgccaacccggacatttatttgtgcggtccgccgggcatgatcgatgccgcctgcgagctggtacgcagccgcggtatccccggcgaacaggtcttcttcgaaaaattcctgccgtccggggcggcctga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J23119_sequence/1
type: Sequence
persistent identity: BBa J23119 sequence
display id: BBa_J23119_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacagctagctcagtcctaggtataatgctagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1390005_sequence/1
type: Sequence
persistent identity: BBa K1390005 sequence
display id: BBa_K1390005_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgagcatgttaggagaaagacgccgcggtctgaccgatccggaaatggcggccgtcattttgaaggcgcttcctgaagctccgctggacggcaacaacaagatgggttatttcgtcaccccccgctggaaacgcttgacggaatatgaagccctgaccgtttatgcgcagcccaacgccgactggatcgccggcggcctggactggggcgactggacccagaaattccacggcggccgcccttcctggggcaacgagaccacggagctgcgcaccgtcgactggttcaagcaccgtgacccgctccgccgttggcatgcgccgtacgtcaaggacaaggccgaggaatggcgctacaccgaccgcttcctgcaaggttactccgccgacggtcagatccgggcgatgaacccgacctggcgggacgagttcatcaaccggtattggggcgccttcctgttcaacgaatacggattgttcaacgctcattcgcagggcgcccgggaggcgctgtcggacgtaacccgcgtcagcctggctttctggggcttcgacaagatcgacatcgcccagatgatccaactcgaacggggtttcctcgccaagatcgtacccggtttcgacgagtccacagcggtgccgaaggccgaatggacgaacggggaggtctacaagagcgcccgtctggccgtggaagggctgtggcaggaggtgttcgactggaacgagagcgctttctcggtgcacgccgtctatgacgcgctgttcggtcagttcgtccgccgcgagttctttcagcggctggctccccgcttcggcgacaatctgacgccattcttcatcaaccaggcccagacatacttccagatcgccaagcagggcgtacaggatctgtattacaactgtctgggtgacgatccggagttcagcgattacaaccgtaccgtgatgcgcaactggaccggcaagtggctggagcccacgatcgccgctctgcgcgacttcatggggctgtttgcgaagctgccggcgggcaccactgacaaggaagaaatcaccgcgtccctgtaccgggtggtcgacgactggatcgaggactacgccagcaggatcgacttcaaggcggaccgcgatcagatcgttaaagcggttctggcaggattgaaatga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1675001_sequence/1
type: Sequence
persistent identity: BBa K1675001 sequence
display id: BBa_K1675001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggagatctcctggggccgcgcgctatggcgcaattttttgggccagtcccccgactggtacaaactcgccctcatcattttcttaatcgtaaacccgttaattttcctcatcagccctttcgtcgcgggctggttgctggtagcggaatttattttcactctggcgatggccctgaaatgctacccgctgctccccggtggtctgttggctatcgaagcggtattcatcggcatgaccagcgcggaacacgtccgtgaagaggtggcggcaaatcttgaagtcttgctgttactgatgtttatggtggcgggtatctattttatgaaacagctattgctgttcatatttacccgtttgctgttaagcattcgctccaaaatgctgctgtcgctctctttttgcgtggcggcggcgttcctctccgcgttcctcgatgccttaaccgtcgtggcggtggtgatcagcgttgcagtcggtttttatggtatttatcaccgcgtagcctcttcccgtactgaagataccgacctgcaagacgatagtcatatcgacaagcattacaaagtggttctggaacagttccgtggctttctgcgtagcctgatgatgcatgccggtgtcggcactgcattaggcggcgtgatgaccatggtgggcgaaccacagaacctgatcatcgctaaagcggctggctggcattttggcgatttcttcctgcgcatgtcgccggtgaccgttccggttctgatttgtggcctgttaacctgcctgctggtagagaagctgcgttggtttggctacggtgaaacgctgccggagaaagtccgcgaagtgttgcaacagtttgacgatcaaagccgccaccagcgtacccgtcaggataaaatccgtctgattgtccaggcgattattggcgtctggctggtgactgcgctggcgttgcatctggcggaagttggcttgattggtttgtcagtcattattctggcgacatcattgaccggtgtcaccgatgaacatgctatcggtaaagccttcaccgaatctctgccattcaccgcactgttgacggtgtttttctcggtagtcgcggtgattatcgaccaacaactgttttcgccaattattcagtttgtgttgcaggcatcggaacatgctcagctgtcgctgttctatattttcaacggtctgctgtcatccatttcggataacgtcttcgtggggacgatttatatcaacgaagcgaaagcggcaatggaaagtggcgctatcacgttgaagcaatacgagctgctggcggtcgccattaataccggtaccaatctgccctccgtcgctacgccgaacggtcaggctgcgttcctgttcctgctgacctctgcactcgcgccattgattcgcctctcttatggccgcatggtgtggatggccctgccttacaccctcgtactgacactcgtcggcttgctctgcgtcgagtttacgcttgcccctgtaaccgaatggtttatgcaaatgggctggatagcaacgctttga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1390001_sequence/1
type: Sequence
persistent identity: BBa K1390001 sequence
display id: BBa_K1390001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgagcgtaaacagcaacgcatacgacgccggcatcatgggcctgaaaggcaaggacttcgccgatcagttctttgccgacgaaaaccaagtggtccatgaaagcgacacggtcgttctggtcctcaagaagtcggacgagatcaatacctttatcgaggagatccttctgacggactacaagaagaacgtcaatccgacggtaaacgtggaagaccgcgcgggttactggtggatcaaggccaacggcaagatcgaggtcgattgcgacgagatttccgagctgttggggcggcagttcaacgtctacgacttcctcgtcgacgtttcctccaccatcggccgggcctataccctgggcaacaagttcaccattaccagtgagctgatgggcctggaccgcaagctcgaagactatcacgcttaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0032_sequence/1
type: Sequence
persistent identity: BBa B0032 sequence
display id: BBa_B0032_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacacaggaaag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1390004_sequence/1
type: Sequence
persistent identity: BBa K1390004 sequence
display id: BBa_K1390004_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggcacttagcaccgcaaccaaggccgcgacggacgcgctggctgccaatcgggcacccaccagcgtgaatgcacaggaagtgcaccgttggctccagagcttcaactgggatttcaagaacaaccggaccaagtacgccaccaagtacaagatggcgaacgagaccaaggaacagttcaagctgatcgccaaggaatatgcgcgcatggaggcagtcaaggacgaaaggcagttcggtagcctgcaagatgcgctgacccgcctcaacgccggtgttcgcgttcatccgaagtggaacgagaccatgaaagtggtttcgaacttcctggaagtgggcgaatacaacgccatcgccgctaccgggatgctgtgggattccgcccaggcggcggaacagaagaacggctatctggcccaggtgttggatgaaatccgccacacccaccagtgtgcctacgtcaactactacttcgcgaagaacggccaggacccggccggtcacaacgatgctcgccgcacccgtaccatcggtccgctgtggaagggcatgaagcgcgtgttttccgacggcttcatttccggcgacgccgtggaatgctccctcaacctgcaactggtgggtgaggcctgcttcaccaatccgctgatcgtcgcagtgaccgaatgggctgccgccaacggcgatgaaatcaccccgacggtgttcctgtcgatcgagaccgacgaactgcgccacatggccaacggttaccagaccgtcgtttccatcgccaacgatccggcttccgccaagtatctcaacacggacctgaacaacgccttctggacccagcagaagtacttcacgccggtgttgggcatgctgttcgagtatggctccaagttcaaggtcgagccgtgggtcaagacgtggaaccgctgggtgtacgaggactggggcggcatctggatcggccgtctgggcaagtacggggtggagtcgccgcgcagcctcaaggacgccaagcaggacgcttactgggctcaccacgacctgtatctgctggcttatgcgctgtggccgaccggcttcttccgtctggcgctgccggatcaggaagaaatggagtggttcgaggccaactaccccggctggtacgaccactacggcaagatctacgaggaatggcgcgcccgcggttgcgaggatccgtcctcgggcttcatcccgctgatgtggttcatcgaaaacaaccatcccatctacatcgatcgcgtgtcgcaagtgccgttctgcccgagcttggccaagggcgccagcaccctgcgcgtgcacgagtacaacggccagatgcacaccttcagcgaccagtggggcgagcgcatgtggctggccgagccggagcgctacgagtgccagaacatcttcgaacagtacgaaggacgcgaactgtcggaagtgatcgccgaactgcacgggctgcgcagtgatggcaagaccctgatcgcccagccgcatgtccgtggcgacaagctgtggacgttggacgatatcaaacgcctgaactgcgtcttcaagaacccggtgaaggcattcaattga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1390006_sequence/1
type: Sequence
persistent identity: BBa K1390006 sequence
display id: BBa_K1390006_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggcgaaactgggtatacacagcaacgacacccgcgacgcctgggtgaacaagatcgcgcagctcaacaccctggaaaaagcggccgagatgctgaagcagttccggatggaccacaccacgccgttccgcaacagctacgaactggacaacgactacctctggatcgaggccaagctcgaagagaaggtcgccgtcctcaaggcacgcgccttcaacgaggtggacttccgtcataagaccgctttcggcgaggatgccaagtccgttctggacggcaccgtcgcgaagatgaacgcggccaaggacaagtgggaggcggagaagatccatatcggtttccgccaggcctacaagccgccgatcatgccggtgaactatttcctggacggcgagcgtcagttggggacccggctgatggaactgcgcaacctcaactactacgacacgccgctggaagaactgcgcaaacagcgcggtgtgcgggtggtgcatctgcaatcgccgtga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0011_sequence/1
type: Sequence
persistent identity: BBa R0011 sequence
display id: BBa_R0011_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcaca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0015_sequence/1
type: Sequence
persistent identity: BBa B0015 sequence
display id: BBa_B0015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z