//regulation/constitutive
type: Collection
persistent identity: regulation constitutive
display id: regulation_constitutive
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
owned by: james
owned by: myers
member: BBa_B0071
member: BBa_B0041
member: BBa_K823006
member: BBa_M13501
member: RBS-GsiB
member: BBa_K1585103
member: LacZ prom
member: BBa_B2017
member: BBa_J23114
member: BBa_K777002
member: BBa_K1895003
member: BBa_K256020
member: BBa_J61106
member: BBa_J34803
member: BBa_J23102
member: BBa_J61118
member: BBa_M13508
member: BBa_J64028
member: BBa_K777104
member: BBa_J44002
member: BBa_K112707
member: KP3
member: R37
member: BBa_B2040
member: BBa_J23108
member: BBa_K823011
member: BBa_K1468002
member: BBa_J23101
member: BBa_K124000
member: BBa_B2022
member: BBa_K1330002
member: BBa_J23109
member: BBa_K1585115
member: T7 RBS
member: BBa_K1824896
member: BBa_J61147
member: BBa_K1468001
member: BBa_K1585102
member: BBa_J64022
member: BBa_K823005
member: BBa_J61117
member: BBa_M13502
member: BBa_J34814
member: BBa_K1585116
member: BBa_K112706
member: BBa_J64808
member: BBa_J15001
member: Kozak & st
member: BBa_J23115
member: BBa_B0036
member: BBa_I742153
member: BBa_K137029
member: BBa_B0070
member: BBa_J64015
member: BBa_K256002
member: BBa_J61110
member: BBa_J61140
member: BBa_M13103
member: BBa_J61123
member: Bla
member: BBa_J61136
member: BBa_M13108
member: pT7
member: BBa_K777105
member: BBa_J61119
member: BBa_B0077
member: BBa_B0034
member: BBa_B0064
member: BBa_K088007
member: BBa_J61112
member: BBa_K292001
member: BBa_M13101

BBa_K137029
type: ComponentDefinition
persistent identity: BBa K137029
display id: BBa_K137029
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: constitutive promoter with (TA)10 between -10 and -35 elements
creator: Allen Lin
created: 2008-07-20T11:00:00Z
modified: 2015-05-08T01:10:09Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _187_
m user id: 0
owner id: 3112
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: constitutive promoter with (TA)10 between -10 and -35 elements
mutable notes: With 10 TA repeats between the -10 and -35 elements, the distance between the two elements is optimal for the sigma factor to bind to the promoter. Thus, the promoter should be in the 'on' state.
mutable provenance: This part was PCR amplified from two synthesized primers that bound to each other.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: TA repeat
sequence annotation: -10 element
sequence: https://synbiohub.org/public/igem/BBa_K137029_sequence/1

TA repeat
type: SequenceAnnotation
persistent identity: annotation1967765
display id: annotation1967765
version: 1
top level: BBa_K137029
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K137029/annotation1967765/range1967765/1

https://synbiohub.org/public/igem/BBa_K137029/annotation1967765/range1967765/1
type: Range
persistent identity: range1967765
display id: range1967765
version: 1
direction: direction/forward
top level: BBa_K137029
start: 7
end: 22
orientation: inline

-10 element
type: SequenceAnnotation
persistent identity: annotation1967764
display id: annotation1967764
version: 1
top level: BBa_K137029
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K137029/annotation1967764/range1967764/1

https://synbiohub.org/public/igem/BBa_K137029/annotation1967764/range1967764/1
type: Range
persistent identity: range1967764
display id: range1967764
version: 1
direction: direction/forward
top level: BBa_K137029
start: 23
end: 28
orientation: inline

BBa_K256002
type: ComponentDefinition
persistent identity: BBa K256002
display id: BBa_K256002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: J23101:GFP
creator: iGEM09_NTU-Singapore
created: 2009-10-15T11:00:00Z
modified: 2015-05-08T01:11:40Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _359_
m user id: 0
owner id: 4864
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: GFP Reporter
mutable notes: --
mutable provenance: --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_B0012
component: BBa_E0040
component: BBa_B0010
component: BBa_J23101
component: BBa_B0034
sequence annotation: BBa_B0010
sequence annotation: BBa_E0040
sequence annotation: BBa_J23101
sequence annotation: BBa_B0012
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K256002_sequence/1

BBa_B0012
type: Component
persistent identity: component2056722
display id: component2056722
version: 1
top level: BBa_K256002
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_E0040
type: Component
persistent identity: component2056719
display id: component2056719
version: 1
top level: BBa_K256002
access: public
definition: https://synbiohub.org/public/igem/BBa_E0040/1

BBa_B0010
type: Component
persistent identity: component2056720
display id: component2056720
version: 1
top level: BBa_K256002
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_J23101
type: Component
persistent identity: component2056715
display id: component2056715
version: 1
top level: BBa_K256002
access: public
definition: BBa_J23101

BBa_B0034
type: Component
persistent identity: component2056717
display id: component2056717
version: 1
top level: BBa_K256002
access: public
definition: BBa_B0034

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2056720
display id: annotation2056720
version: 1
top level: BBa_K256002
location: https://synbiohub.org/public/igem/BBa_K256002/annotation2056720/range2056720/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K256002/annotation2056720/range2056720/1
type: Range
persistent identity: range2056720
display id: range2056720
version: 1
direction: direction/forward
top level: BBa_K256002
start: 790
end: 869
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation2056719
display id: annotation2056719
version: 1
top level: BBa_K256002
location: https://synbiohub.org/public/igem/BBa_K256002/annotation2056719/range2056719/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_K256002/annotation2056719/range2056719/1
type: Range
persistent identity: range2056719
display id: range2056719
version: 1
direction: direction/reverse
top level: BBa_K256002
start: 62
end: 781
orientation: inline

BBa_J23101
type: SequenceAnnotation
persistent identity: annotation2056715
display id: annotation2056715
version: 1
top level: BBa_K256002
location: https://synbiohub.org/public/igem/BBa_K256002/annotation2056715/range2056715/1
component: BBa_J23101

https://synbiohub.org/public/igem/BBa_K256002/annotation2056715/range2056715/1
type: Range
persistent identity: range2056715
display id: range2056715
version: 1
direction: direction/reverse
top level: BBa_K256002
start: 1
end: 35
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2056722
display id: annotation2056722
version: 1
top level: BBa_K256002
location: https://synbiohub.org/public/igem/BBa_K256002/annotation2056722/range2056722/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K256002/annotation2056722/range2056722/1
type: Range
persistent identity: range2056722
display id: range2056722
version: 1
direction: direction/forward
top level: BBa_K256002
start: 878
end: 918
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2056717
display id: annotation2056717
version: 1
top level: BBa_K256002
location: https://synbiohub.org/public/igem/BBa_K256002/annotation2056717/range2056717/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K256002/annotation2056717/range2056717/1
type: Range
persistent identity: range2056717
display id: range2056717
version: 1
direction: direction/reverse
top level: BBa_K256002
start: 44
end: 55
orientation: inline

BBa_K1585106
type: ComponentDefinition
persistent identity: BBa K1585106
display id: BBa_K1585106
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Anderson Promoter with lacI binding site
creator: Tobias Schwanemann
created: 2015-08-31T11:00:00Z
modified: 2015-09-01T02:01:39Z
discontinued: false
dominant: false
experience: experience/Fails
group u list: _2002_
m user id: 24474
owner id: 24474
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Promoters of the Anderson Promoter Collection with lacI binding site derived from [http://parts.igem.org/Part:BBa_R0010 R_0010] to make them [http://openwetware.org/wiki/IPTG IPTG] iducible.
mutable notes: We choosed lacI-binding-site of part BBa_R0010 because we assumed older parts would be more reliable. The part is shipped in backbone [http://parts.igem.org/Part:BBa_J61002 J61002] because the functionality is directly visible by adding IPTG. RFP ([http://parts.igem.org/Part:BBa_E1010 E1010]) should get expressed then.
mutable provenance: Assambly of [http://parts.igem.org/Part:BBa_J23106 J23106] and lacI-binding-site of [http://parts.igem.org/Part:BBa_R0010 R_0010] The "lacI binding site" of Part [http://parts.igem.org/Part:BBa_R0010 R_0010] differs from lacO from Part [http://parts.igem.org/Part:BBa_J33207 BBa_J33207].
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
component: BBa_K1585999
component: BBa_J23106
sequence annotation: BBa_J23106
sequence annotation: BBa_K1585999
sequence: https://synbiohub.org/public/igem/BBa_K1585106_sequence/1

BBa_K1585999
type: Component
persistent identity: component2441698
display id: component2441698
version: 1
top level: BBa_K1585106
access: public
definition: https://synbiohub.org/public/igem/BBa_K1585999/1

BBa_J23106
type: Component
persistent identity: component2441696
display id: component2441696
version: 1
top level: BBa_K1585106
access: public
definition: BBa_J23106

BBa_J23106
type: SequenceAnnotation
persistent identity: annotation2441696
display id: annotation2441696
version: 1
top level: BBa_K1585106
location: https://synbiohub.org/public/igem/BBa_K1585106/annotation2441696/range2441696/1
component: BBa_J23106

https://synbiohub.org/public/igem/BBa_K1585106/annotation2441696/range2441696/1
type: Range
persistent identity: range2441696
display id: range2441696
version: 1
direction: direction/reverse
top level: BBa_K1585106
start: 1
end: 35
orientation: inline

BBa_K1585999
type: SequenceAnnotation
persistent identity: annotation2441698
display id: annotation2441698
version: 1
top level: BBa_K1585106
location: https://synbiohub.org/public/igem/BBa_K1585106/annotation2441698/range2441698/1
component: BBa_K1585999

https://synbiohub.org/public/igem/BBa_K1585106/annotation2441698/range2441698/1
type: Range
persistent identity: range2441698
display id: range2441698
version: 1
direction: direction/reverse
top level: BBa_K1585106
start: 44
end: 78
orientation: inline

BBa_R0180
type: ComponentDefinition
persistent identity: BBa R0180
display id: BBa_R0180
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: T7 RNAP promoter
creator: Barry Canton
created: 2005-09-25T11:00:00Z
modified: 2015-05-08T01:14:15Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_6_
m user id: 0
owner id: 135
owning group id: 6
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: T7 promoter with an A->T mutation at the -16 position of the consensus sequence
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_R0180_sequence/1

BBa_M13101
type: ComponentDefinition
persistent identity: BBa M13101
display id: BBa_M13101
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: M13K07 gene I promoter
creator: Natalie Kuldell
created: 2006-12-15T12:00:00Z
modified: 2015-05-08T01:13:55Z
discontinued: false
dominant: false
group u list: _45_
m user id: 0
owner id: 314
owning group id: 1
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This part is from the bacteriophage M13 genome, bp 3086-3132 in M13K07. It directs transcription of M13 gene VII (BBa_M13507, BBa_M13007). Its identity as a promoter was described in Gene (1980) 11:129-148 based on -10,-35 homology and fragment of genome able to bind RNAP.
mutable notes: none
mutable provenance: New England Biolabs M13K07. Sequenced in 2002 by L. Panganaban.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_M13101_sequence/1

BBa_J61109
type: ComponentDefinition
persistent identity: BBa J61109
display id: BBa_J61109
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ribosome Binding Site Family Member
creator: John Anderson
created: 2007-04-23T11:00:00Z
modified: 2015-08-31T02:03:00Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable description: {{JCA_Arkin_RBSFamily}}
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J61109_sequence/1

BBa_M13507
type: ComponentDefinition
persistent identity: BBa M13507
display id: BBa_M13507
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: M13KO7 gene VII RBS
creator: Natalie Kuldell
created: 2006-12-14T12:00:00Z
modified: 2015-05-08T01:13:56Z
discontinued: false
dominant: true
experience: experience/None
group u list: _45_
m user id: 0
owner id: 314
owning group id: 1
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This RBS is part of the bacteriophage M13 genome, initiating translation of the gene VII message (BBa_M13007). The part aligns with base pairs 1092-1107 in M13K07 genome. It was identified as the RBS for gene VII message by Wezenbeek et al in Gene (1980) 11: 129-148
mutable notes: New England Biolabs M13K07. Sequenced in 2002 by L. Panganaban
mutable provenance: New England Biolabs M13K07. Sequenced in 2002 by L. Panganaban
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_M13507_sequence/1

BBa_J15001
type: ComponentDefinition
persistent identity: BBa J15001
display id: BBa_J15001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: strong synthetic E. coli ribosome binding site with SacI site.
creator: Chris French
created: 2007-07-12T11:00:00Z
modified: 2015-08-31T04:08:32Z
discontinued: false
dominant: false
experience: experience/None
group u list: _163_
m user id: 0
owner id: 837
owning group id: 163
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is a strong synthetic E. coli ribosome binding site. It is synthesised as two complementary oligonucleotides rather than being incorporated into a biobrick plasmid. It incorporates a SacI site overlapping the XbaI site, which is preserved when it is added to any other biobrick. This allows easy detection of the RBS after it has been added upstream of a biobrick coding sequence in a plasmid vector.
mutable notes: Note the presence of a SacI site overlapping the XbaI site, which is preserved when this biobrick is added to any other biobrick. At the time of writing, this biobrick is added as a short piece of DNA composed of two complementary oligonucleotides rather than being incorporated into a biobrick cloning vector by itself. It can be added upstream of a biobrick coding sequence, and its presence can easily be detected in miniprep DNA on a gel by using a SacI-SpeI or similar digest.
mutable provenance: Synthetic.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: SacI
sequence annotation: rbs
sequence: https://synbiohub.org/public/igem/BBa_J15001_sequence/1

SacI
type: SequenceAnnotation
persistent identity: annotation1938045
display id: annotation1938045
version: 1
top level: BBa_J15001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J15001/annotation1938045/range1938045/1

https://synbiohub.org/public/igem/BBa_J15001/annotation1938045/range1938045/1
type: Range
persistent identity: range1938045
display id: range1938045
version: 1
direction: direction/forward
top level: BBa_J15001
start: 1
end: 3
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation1938046
display id: annotation1938046
version: 1
top level: BBa_J15001
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_J15001/annotation1938046/range1938046/1

https://synbiohub.org/public/igem/BBa_J15001/annotation1938046/range1938046/1
type: Range
persistent identity: range1938046
display id: range1938046
version: 1
direction: direction/forward
top level: BBa_J15001
start: 4
end: 10
orientation: inline

BBa_J61116
type: ComponentDefinition
persistent identity: BBa J61116
display id: BBa_J61116
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ribosome Binding Site Family Member
creator: John Anderson
created: 2007-04-23T11:00:00Z
modified: 2015-08-31T02:03:00Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable description: {{JCA_Arkin_RBSFamily}}
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J61116_sequence/1

BBa_M13108
type: ComponentDefinition
persistent identity: BBa M13108
display id: BBa_M13108
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: M13K07 gene VIII promoter
creator: Natalie Kuldell
created: 2006-12-15T12:00:00Z
modified: 2015-05-08T01:13:55Z
discontinued: false
dominant: true
group u list: _45_
m user id: 0
owner id: 314
owning group id: 1
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This part is from the bacteriophage M13 genome, bp 1155-1201 in M13K07. It directs transcription of M13 gene VIII (BBa_M13508, BBa_M13008). Its identity as a promoter was described in Gene (1980) 11:129-148 based on -10,-35 homology and fragment of genome able to bind RNAP.
mutable notes: none
mutable provenance: New England Biolabs M13K07. Sequenced in 2002 by L. Panganaban.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_M13108_sequence/1

BBa_J64018
type: ComponentDefinition
persistent identity: BBa J64018
display id: BBa_J64018
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: invJ RBS
creator: Dan Widmaier
created: 2008-01-16T12:00:00Z
modified: 2015-08-31T01:56:26Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _98_
m user id: 0
owner id: 2407
owning group id: 98
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This part is the natural RBS associated with the invJ protein in Salmonella Typhimurium LT2.
mutable notes: This part was not designed with compliance to biobrick standard assembly in mind.
mutable provenance: Salmonella Typhimurium LT2 Genomic DNA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: invJ RBS
sequence: https://synbiohub.org/public/igem/BBa_J64018_sequence/1

invJ RBS
type: SequenceAnnotation
persistent identity: annotation1959617
display id: annotation1959617
version: 1
top level: BBa_J64018
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_J64018/annotation1959617/range1959617/1

https://synbiohub.org/public/igem/BBa_J64018/annotation1959617/range1959617/1
type: Range
persistent identity: range1959617
display id: range1959617
version: 1
direction: direction/forward
top level: BBa_J64018
start: 1
end: 17
orientation: inline

BBa_K082001
type: ComponentDefinition
persistent identity: BBa K082001
display id: BBa_K082001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS medium
creator: Yanting Xue , Binjie Xu
created: 2008-10-27T12:00:00Z
modified: 2015-05-08T01:08:35Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _228_
m user id: 0
owner id: 1560
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Weak1 RBS based on Ron Weiss thesis. Strength is considered relative to BBa_B0030, BBa_B0031, BBa_B0033.
mutable notes: the
mutable provenance: the
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: BBa_K082001
sequence: https://synbiohub.org/public/igem/BBa_K082001_sequence/1

BBa_K082001
type: SequenceAnnotation
persistent identity: annotation1993438
display id: annotation1993438
version: 1
top level: BBa_K082001
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K082001/annotation1993438/range1993438/1

https://synbiohub.org/public/igem/BBa_K082001/annotation1993438/range1993438/1
type: Range
persistent identity: range1993438
display id: range1993438
version: 1
direction: direction/reverse
top level: BBa_K082001
start: 1
end: 15
orientation: inline

BBa_I742156
type: ComponentDefinition
persistent identity: BBa I742156
display id: BBa_I742156
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: crtZ native rbs
creator: Chris French
created: 2007-10-23T11:00:00Z
modified: 2015-08-31T04:08:03Z
discontinued: false
dominant: false
experience: experience/None
group u list: _123_
m user id: 0
owner id: 837
owning group id: 163
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Native ribosome binding site for crtZ (beta-carotene hydroxylase) from Pantoea ananatis (formerly Erwinia uredovora) DSMZ 30080 (ATCC 19321). Accession: D90087.
mutable notes: A virtual part to be added to the coding sequence blunt (without a scar).
mutable provenance: Pantoea ananatis (formerly Erwinia uredovora) DSMZ 30080 (ATCC 19321). Accession: D90087.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_I742156_sequence/1

BBa_K1895002
type: ComponentDefinition
persistent identity: BBa K1895002
display id: BBa_K1895002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: dnaK Promoter
creator: iGEM16_Newcastle
created: 2016-09-05T11:00:00Z
modified: 2016-09-06T04:33:29Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2361_
m user id: 29859
owner id: 29859
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This is the dnaK promoter
mutable notes: [TODO]
mutable provenance: Uniprot
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K1895002_sequence/1

BBa_K137089
type: ComponentDefinition
persistent identity: BBa K137089
display id: BBa_K137089
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: optimized (TA) repeat constitutive promoter with 21 bp between -10 and -35 elements
creator: Allen Lin
created: 2008-08-20T11:00:00Z
modified: 2015-05-08T01:10:10Z
discontinued: false
dominant: false
experience: experience/None
group u list: _187_
m user id: 0
owner id: 3112
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: optimized (TA) repeat constitutive promoter with 21 bp between -10 and -35 elements
mutable notes: None
mutable provenance: PCR
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K137089_sequence/1

BBa_J54200
type: ComponentDefinition
persistent identity: BBa J54200
display id: BBa_J54200
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: lacq_Promoter
creator: Yusaku Nakashima
created: 2006-10-14T11:00:00Z
modified: 2015-08-31T03:48:12Z
discontinued: false
dominant: false
group u list: _76_
m user id: 0
owner id: 762
owning group id: 76
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Promoter that express always.
mutable notes: As promoter
mutable provenance: DNA synthesis
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BamHI
sequence annotation: lacq Promoter
sequence: https://synbiohub.org/public/igem/BBa_J54200_sequence/1

BamHI
type: SequenceAnnotation
persistent identity: annotation1903214
display id: annotation1903214
version: 1
top level: BBa_J54200
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_J54200/annotation1903214/range1903214/1

https://synbiohub.org/public/igem/BBa_J54200/annotation1903214/range1903214/1
type: Range
persistent identity: range1903214
display id: range1903214
version: 1
direction: direction/forward
top level: BBa_J54200
start: 7
end: 13
orientation: inline

lacq Promoter
type: SequenceAnnotation
persistent identity: annotation1903215
display id: annotation1903215
version: 1
top level: BBa_J54200
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J54200/annotation1903215/range1903215/1

https://synbiohub.org/public/igem/BBa_J54200/annotation1903215/range1903215/1
type: Range
persistent identity: range1903215
display id: range1903215
version: 1
direction: direction/forward
top level: BBa_J54200
start: 14
end: 48
orientation: inline

BBa_M13105
type: ComponentDefinition
persistent identity: BBa M13105
display id: BBa_M13105
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: M13K07 gene V promoter
creator: Natalie Kuldell
created: 2006-12-15T12:00:00Z
modified: 2015-05-08T01:13:55Z
discontinued: false
dominant: false
group u list: _45_
m user id: 0
owner id: 314
owning group id: 1
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This part is from the bacteriophage M13 genome, bp 786-835 in M13K07. It directs transcription of M13 gene V (BBa_M13505, BBa_M13005). Its identity as a promoter was described in Gene (1980) 11:129-148 based on -10,-35 homology and fragment of genome able to bind RNAP.
mutable notes: none
mutable provenance: New England Biolabs M13K07. Sequenced in 2002 by L. Panganaban.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_M13105_sequence/1

BBa_J61126
type: ComponentDefinition
persistent identity: BBa J61126
display id: BBa_J61126
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ribosome Binding Site Family Member
creator: John Anderson
created: 2007-04-24T11:00:00Z
modified: 2015-08-31T01:59:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable description: {{JCA_Arkin_RBSFamily}}
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J61126_sequence/1

BBa_K809101
type: ComponentDefinition
persistent identity: BBa K809101
display id: BBa_K809101
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Mitochondrial shuttle plasmid carried mtGFP
creator: Senwei TANG, Qiwu XU
created: 2012-09-19T11:00:00Z
modified: 2015-05-08T01:13:26Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1066_
m user id: 0
owner id: 11943
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: Mitochondrial expression vector, with initial site ori7, up elements of cox2 gene (including promoter and RBS), and mtGFP.
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K809002
component: BBa_K809003
component: BBa_K809001
sequence annotation: BBa_K809001
sequence annotation: BBa_K809003
sequence annotation: BBa_K809002
sequence: https://synbiohub.org/public/igem/BBa_K809101_sequence/1

BBa_K809002
type: Component
persistent identity: component2192205
display id: component2192205
version: 1
top level: BBa_K809101
access: public
definition: https://synbiohub.org/public/igem/BBa_K809002/1

BBa_K809003
type: Component
persistent identity: component2192206
display id: component2192206
version: 1
top level: BBa_K809101
access: public
definition: UPcox1

BBa_K809001
type: Component
persistent identity: component2192208
display id: component2192208
version: 1
top level: BBa_K809101
access: public
definition: https://synbiohub.org/public/igem/BBa_K809001/1

BBa_K809001
type: SequenceAnnotation
persistent identity: annotation2192208
display id: annotation2192208
version: 1
top level: BBa_K809101
location: https://synbiohub.org/public/igem/BBa_K809101/annotation2192208/range2192208/1
component: BBa_K809001

https://synbiohub.org/public/igem/BBa_K809101/annotation2192208/range2192208/1
type: Range
persistent identity: range2192208
display id: range2192208
version: 1
direction: direction/reverse
top level: BBa_K809101
start: 890
end: 1606
orientation: inline

BBa_K809003
type: SequenceAnnotation
persistent identity: annotation2192206
display id: annotation2192206
version: 1
top level: BBa_K809101
location: https://synbiohub.org/public/igem/BBa_K809101/annotation2192206/range2192206/1
component: BBa_K809003

https://synbiohub.org/public/igem/BBa_K809101/annotation2192206/range2192206/1
type: Range
persistent identity: range2192206
display id: range2192206
version: 1
direction: direction/reverse
top level: BBa_K809101
start: 387
end: 883
orientation: inline

BBa_K809002
type: SequenceAnnotation
persistent identity: annotation2192205
display id: annotation2192205
version: 1
top level: BBa_K809101
location: https://synbiohub.org/public/igem/BBa_K809101/annotation2192205/range2192205/1
component: BBa_K809002

https://synbiohub.org/public/igem/BBa_K809101/annotation2192205/range2192205/1
type: Range
persistent identity: range2192205
display id: range2192205
version: 1
direction: direction/reverse
top level: BBa_K809101
start: 1
end: 378
orientation: inline

BBa_J64028
type: ComponentDefinition
persistent identity: BBa J64028
display id: BBa_J64028
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: sopB RBS
creator: Dan Widmaier
created: 2008-01-16T12:00:00Z
modified: 2015-08-31T01:56:26Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _98_
m user id: 0
owner id: 2407
owning group id: 98
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This part is the natural RBS for the sopB gene in the Salmonella Type III Secretion System.
mutable notes: This part was not designed with consideration for biobrick standard assembly in mind. This RBS hasn't been explicitly tested for expression rate in a Fluorescence or B-Gal assay.
mutable provenance: Salmonella LT2 Genomic DNA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: sopB RBS
sequence: https://synbiohub.org/public/igem/BBa_J64028_sequence/1

sopB RBS
type: SequenceAnnotation
persistent identity: annotation1959632
display id: annotation1959632
version: 1
top level: BBa_J64028
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_J64028/annotation1959632/range1959632/1

https://synbiohub.org/public/igem/BBa_J64028/annotation1959632/range1959632/1
type: Range
persistent identity: range1959632
display id: range1959632
version: 1
direction: direction/forward
top level: BBa_J64028
start: 1
end: 20
orientation: inline

BBa_K137088
type: ComponentDefinition
persistent identity: BBa K137088
display id: BBa_K137088
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: optimized (TA) repeat constitutive promoter with 19 bp between -10 and -35 elements
creator: Allen Lin
created: 2008-08-20T11:00:00Z
modified: 2015-05-08T01:10:10Z
discontinued: false
dominant: false
experience: experience/None
group u list: _187_
m user id: 0
owner id: 3112
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: optimized (TA) repeat constitutive promoter with 19 bp between -10 and -35 elements
mutable notes: None
mutable provenance: PCR
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K137088_sequence/1

Kozak & st
type: ComponentDefinition
persistent identity: BBa J63003
display id: BBa_J63003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: designed yeast Kozak sequence
creator: Caroline Ajo-Franklin
created: 2006-10-10T11:00:00Z
modified: 2015-08-31T01:56:26Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _97_
m user id: 0
owner id: 545
owning group id: 97
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: consensus Kozak sequence and start codon
mutable notes: designed such that fusion using fusion bricks assembly method leads to in frame translation
mutable provenance: consensus Kozak and start built from oligonucleotides
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: start codon
sequence: https://synbiohub.org/public/igem/BBa_J63003_sequence/1

start codon
type: SequenceAnnotation
persistent identity: annotation1902852
display id: annotation1902852
version: 1
top level: Kozak & st
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_J63003/annotation1902852/range1902852/1

https://synbiohub.org/public/igem/BBa_J63003/annotation1902852/range1902852/1
type: Range
persistent identity: range1902852
display id: range1902852
version: 1
direction: direction/forward
top level: Kozak & st
start: 13
end: 15
orientation: inline

BBa_K823013
type: ComponentDefinition
persistent identity: BBa K823013
display id: BBa_K823013
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Anderson promoter J23117
creator: Korinna Kraft
created: 2012-09-07T11:00:00Z
modified: 2015-07-07T11:51:22Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1081_
m user id: 4206
owner id: 12081
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Anderson promoter J23117 without RFP in backbone vector pSB1C3 to easily fuse the promoter with other reporters e.g. the lux operon.
mutable notes: no considerations
mutable provenance: Partsregistry
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -35 box
sequence annotation: -10 box
sequence annotation: J23117
sequence: https://synbiohub.org/public/igem/BBa_K823013_sequence/1

-35 box
type: SequenceAnnotation
persistent identity: annotation2182544
display id: annotation2182544
version: 1
top level: BBa_K823013
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K823013/annotation2182544/range2182544/1

https://synbiohub.org/public/igem/BBa_K823013/annotation2182544/range2182544/1
type: Range
persistent identity: range2182544
display id: range2182544
version: 1
direction: direction/forward
top level: BBa_K823013
start: 1
end: 6
orientation: inline

-10 box
type: SequenceAnnotation
persistent identity: annotation2182545
display id: annotation2182545
version: 1
top level: BBa_K823013
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K823013/annotation2182545/range2182545/1

https://synbiohub.org/public/igem/BBa_K823013/annotation2182545/range2182545/1
type: Range
persistent identity: range2182545
display id: range2182545
version: 1
direction: direction/forward
top level: BBa_K823013
start: 24
end: 29
orientation: inline

J23117
type: SequenceAnnotation
persistent identity: annotation2182543
display id: annotation2182543
version: 1
top level: BBa_K823013
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K823013/annotation2182543/range2182543/1

https://synbiohub.org/public/igem/BBa_K823013/annotation2182543/range2182543/1
type: Range
persistent identity: range2182543
display id: range2182543
version: 1
direction: direction/forward
top level: BBa_K823013
start: 1
end: 35
orientation: inline

BBa_J61100
type: ComponentDefinition
persistent identity: BBa J61100
display id: BBa_J61100
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ribosome Binding Site Family Member
creator: John Anderson
created: 2007-01-28T12:00:00Z
modified: 2015-08-31T02:03:00Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable description: {{JCA_Arkin_RBSFamily}}
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J61100_sequence/1

BBa_K1330002
type: ComponentDefinition
persistent identity: BBa K1330002
display id: BBa_K1330002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Constitutive promoter (J23105)
creator: Kristian Jensen
created: 2014-09-29T11:00:00Z
modified: 2015-05-08T01:09:55Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1705_
m user id: 0
owner id: 20126
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: t
mutable notes: t
mutable provenance: t
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K1330002_sequence/1

BBa_B0070
type: ComponentDefinition
persistent identity: BBa B0070
display id: BBa_B0070
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Specialized RBS modified from that of B0036 (Brink et al.)
creator: Barry Canton
created: 2006-03-03T12:00:00Z
modified: 2015-08-31T04:07:21Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_6_
m user id: 0
owner id: 135
owning group id: 6
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This RBS has the modified Shine-Dalgarno sequence, it has a reduced secondary structure relative to using the actual Brink sequence with mixed connective sites flanking it. the spacing between the SD and the ATG is the same as that used by Brink et al. even though it is non-optimal for E. coli
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_B0070_sequence/1

BBa_K777008
type: ComponentDefinition
persistent identity: BBa K777008
display id: BBa_K777008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Tar receptor under the control of constitutive promoter J23114
creator: Bianca Genenncher
created: 2012-09-20T11:00:00Z
modified: 2015-05-08T01:13:16Z
discontinued: false
dominant: false
group u list: _1029_
m user id: 0
owner id: 11946
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: The transmembrane chemoreceptor Tar of E. coli mediates chemotaxis towards aspartate and exists as a functional homodimer. Here we used a set of 8 constitutive [http://partsregistry.org/Part:BBa_J23114 Anderson promoters] from the 2006 Berkeley group to test how different levels of constitutive ''Tar'' expression affect the chemotaxis of ''E. coli''.
mutable notes: * Genomic sequence was amplified using the following primers. ** Primers PCR: Base pairs in caps represent the respective restriction sites followed by either prefix or suffix. Primers were provided by SIGMA. *** Fwd: Tar for + prefix: 5?? actGAATTCgcggccgctTCTAGAtgattaaccgtatccgcgtagtc 3?? *** Rev: Tar rev + suffix: 5?? tcCTGCAGcggccgctACTAGTcaaaatgtttcccagtttggatc 3?? * One XbaI site was found at position 420. The restriction site was changed from TCTAGt to CCTAGt via QuikChange PCR. ** Primers QuikChange (QC): Primers were provided by SIGMA. *** Fwd: TarQC for: 5?? ACTGATTGATTAcCTAGATTATGGCAATACTGGAG 3?? *** Rev: TarQC rev: 5?? TGCCATAATCTAGgTAATCAATCAGTTCAGTTAAC 3?? (the uncapitalized letter induces the mutation for removal of the XbaI site)
mutable provenance: *The part was amplified from genomic DNA of ''E. coli'' str. K-12 substr. DH10B, complete genome (CP000948.1). *J23114 information was taken from the partsregistry and physical DNA from the 2012 distribution kit.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K777000
component: BBa_J23114
sequence annotation: BBa_J23114
sequence annotation: BBa_K777000
sequence: https://synbiohub.org/public/igem/BBa_K777008_sequence/1

BBa_K777000
type: Component
persistent identity: component2189408
display id: component2189408
version: 1
top level: BBa_K777008
access: public
definition: BBa_K777000

BBa_J23114
type: Component
persistent identity: component2189404
display id: component2189404
version: 1
top level: BBa_K777008
access: public
definition: BBa_J23114

BBa_J23114
type: SequenceAnnotation
persistent identity: annotation2189404
display id: annotation2189404
version: 1
top level: BBa_K777008
location: https://synbiohub.org/public/igem/BBa_K777008/annotation2189404/range2189404/1
component: BBa_J23114

https://synbiohub.org/public/igem/BBa_K777008/annotation2189404/range2189404/1
type: Range
persistent identity: range2189404
display id: range2189404
version: 1
direction: direction/reverse
top level: BBa_K777008
start: 1
end: 35
orientation: inline

BBa_K777000
type: SequenceAnnotation
persistent identity: annotation2189408
display id: annotation2189408
version: 1
top level: BBa_K777008
location: https://synbiohub.org/public/igem/BBa_K777008/annotation2189408/range2189408/1
component: BBa_K777000

https://synbiohub.org/public/igem/BBa_K777008/annotation2189408/range2189408/1
type: Range
persistent identity: range2189408
display id: range2189408
version: 1
direction: direction/reverse
top level: BBa_K777008
start: 42
end: 1703
orientation: inline

creB RBS
type: ComponentDefinition
persistent identity: BBa J64968
display id: BBa_J64968
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS on creB
creator: Kristopher Kuchenbecker
created: 2007-03-19T12:00:00Z
modified: 2015-05-08T01:08:17Z
discontinued: false
dominant: false
experience: experience/None
group u list: _98_
m user id: 0
owner id: 1429
owning group id: 98
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Ribosome Binding Site
mutable notes: RBS
mutable provenance: ''E. coli'' K-12 genome
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J64968_sequence/1

BBa_J61132
type: ComponentDefinition
persistent identity: BBa J61132
display id: BBa_J61132
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ribosome Binding Site Family Member
creator: John Anderson
created: 2007-04-24T11:00:00Z
modified: 2015-08-31T01:59:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable description: {{JCA_Arkin_RBSFamily}}
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J61132_sequence/1

BBa_J44002
type: ComponentDefinition
persistent identity: BBa J44002
display id: BBa_J44002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pBAD reverse
creator: Brad Ogden
created: 2006-08-15T11:00:00Z
modified: 2015-08-31T04:08:48Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _71_
m user id: 0
owner id: 811
owning group id: 71
sample status: In stock
status: status/Available
bookmark: true
mutable description: This is the pBAD promoter (BBa_I13453) in the opposite orientation. It can be used to drive transcription in the direction of suffix to prefix.
mutable notes: None.
mutable provenance: Cloned from synthetic oligonucleotides.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: promoter
sequence: https://synbiohub.org/public/igem/BBa_J44002_sequence/1

promoter
type: SequenceAnnotation
persistent identity: annotation2002776
display id: annotation2002776
version: 1
top level: BBa_J44002
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J44002/annotation2002776/range2002776/1

https://synbiohub.org/public/igem/BBa_J44002/annotation2002776/range2002776/1
type: Range
persistent identity: range2002776
display id: range2002776
version: 1
direction: direction/reverse
top level: BBa_J44002
start: 1
end: 130
orientation: inline

BBa_K122000
type: ComponentDefinition
persistent identity: BBa K122000
display id: BBa_K122000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pPGK1
creator: David Ouyang
created: 2008-10-20T11:00:00Z
modified: 2015-05-08T01:09:42Z
discontinued: false
dominant: false
experience: experience/None
group u list: _218_
m user id: 0
owner id: 3326
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This is the 1500 bp upstream of the yeast PGK1 coding region. This is a putative constitutive promoter.
mutable notes: Source: Saccharomyces cerevisiae genomic DNA.
mutable provenance: This part was obtained from yeast genomic DNA.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K122000_sequence/1

BBa_K137086
type: ComponentDefinition
persistent identity: BBa K137086
display id: BBa_K137086
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: optimized (TA) repeat constitutive promoter with 15 bp between -10 and -35 elements
creator: Allen Lin
created: 2008-08-20T11:00:00Z
modified: 2015-05-08T01:10:10Z
discontinued: false
dominant: false
experience: experience/None
group u list: _187_
m user id: 0
owner id: 3112
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: optimized (TA) repeat constitutive promoter with 15 bp between -10 and -35 elements
mutable notes: None
mutable provenance: PCR
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K137086_sequence/1

BBa_K823006
type: ComponentDefinition
persistent identity: BBa K823006
display id: BBa_K823006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Anderson promoter J23102
creator: Korinna Kraft
created: 2012-09-07T11:00:00Z
modified: 2015-07-07T11:45:05Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1081_
m user id: 4206
owner id: 12081
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Anderson promoter J23102 without RFP in backbone vector pSB1C3 to easily fuse the promoter with other reporters e.g. the lux operon.
mutable notes: no considerations
mutable provenance: Partsregistry
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: J23102
sequence annotation: -10 box
sequence annotation: -35 box
sequence: https://synbiohub.org/public/igem/BBa_K823006_sequence/1

J23102
type: SequenceAnnotation
persistent identity: annotation2182512
display id: annotation2182512
version: 1
top level: BBa_K823006
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K823006/annotation2182512/range2182512/1

https://synbiohub.org/public/igem/BBa_K823006/annotation2182512/range2182512/1
type: Range
persistent identity: range2182512
display id: range2182512
version: 1
direction: direction/forward
top level: BBa_K823006
start: 1
end: 35
orientation: inline

-10 box
type: SequenceAnnotation
persistent identity: annotation2182514
display id: annotation2182514
version: 1
top level: BBa_K823006
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K823006/annotation2182514/range2182514/1

https://synbiohub.org/public/igem/BBa_K823006/annotation2182514/range2182514/1
type: Range
persistent identity: range2182514
display id: range2182514
version: 1
direction: direction/forward
top level: BBa_K823006
start: 24
end: 29
orientation: inline

-35 box
type: SequenceAnnotation
persistent identity: annotation2182513
display id: annotation2182513
version: 1
top level: BBa_K823006
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K823006/annotation2182513/range2182513/1

https://synbiohub.org/public/igem/BBa_K823006/annotation2182513/range2182513/1
type: Range
persistent identity: range2182513
display id: range2182513
version: 1
direction: direction/forward
top level: BBa_K823006
start: 1
end: 6
orientation: inline

BBa_J61134
type: ComponentDefinition
persistent identity: BBa J61134
display id: BBa_J61134
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ribosome Binding Site Family Member
creator: John Anderson
created: 2007-04-24T11:00:00Z
modified: 2015-08-31T01:59:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable description: {{JCA_Arkin_RBSFamily}}
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J61134_sequence/1

BBa_I742153
type: ComponentDefinition
persistent identity: BBa I742153
display id: BBa_I742153
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: crtY native rbs
creator: Chris French
created: 2007-10-23T11:00:00Z
modified: 2015-08-31T04:08:03Z
discontinued: false
dominant: false
experience: experience/None
group u list: _123_
m user id: 0
owner id: 837
owning group id: 163
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Native ribosome binding site of crtY (lycopene cyclase) from Pantoea ananatis (formerly Erwinia uredovora) DSMZ 30080 (ATCC 19321). Accession: D90087.
mutable notes: This is a virtual part to be added, blunt-ended, to the coding sequence.
mutable provenance: Pantoea ananatis (formerly Erwinia uredovora) DSMZ 30080 (ATCC 19321). Accession: D90087.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_I742153_sequence/1

BBa_J61119
type: ComponentDefinition
persistent identity: BBa J61119
display id: BBa_J61119
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ribosome Binding Site Family Member
creator: John Anderson
created: 2007-04-24T11:00:00Z
modified: 2015-08-31T01:59:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable description: {{JCA_Arkin_RBSFamily}}
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J61119_sequence/1

T7 0.4
type: ComponentDefinition
persistent identity: BBa B2002
display id: BBa_B2002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: T7 RBS 0.4
creator: Heather Keller
created: 2006-09-27T11:00:00Z
modified: 2015-08-31T04:07:21Z
discontinued: false
dominant: true
experience: experience/None
group u list: _11_
m user id: 0
owner id: 571
owning group id: 10
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is the -20 to -1 region of T7 gp0.7, which contains the native RBS region for this gene. The physical instantiation of this part does not exist. This part is not compatible with Standard Biobricks assembly methods, as the scar created during assembly would interrupt the spacing between the RBS and the start codon of the CDS. Individuals wishing to use this RBS sequence in their assembles should refer to part XXXXX. (Information forthcoming)
mutable notes: The -20 to -1 region was chosen to be representative of the RBS region of the T7 genes for the following reasons: 1) The Shine-Delgarno region is contained within this defined region for all T7 genes. 2) Current literature suggests that when bound to the Translation Start Site (0), the Ribosome covers positions -20 to +13.
mutable provenance: Genome of wild-type bacteriophage T7, positions 2001-2020. Includes the -20 to -1 region of gp0.7.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: SD region
sequence: https://synbiohub.org/public/igem/BBa_B2002_sequence/1

SD region
type: SequenceAnnotation
persistent identity: annotation1902419
display id: annotation1902419
version: 1
top level: T7 0.4
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_B2002/annotation1902419/range1902419/1

https://synbiohub.org/public/igem/BBa_B2002/annotation1902419/range1902419/1
type: Range
persistent identity: range1902419
display id: range1902419
version: 1
direction: direction/forward
top level: T7 0.4
start: 4
end: 10
orientation: inline

BBa_J23118
type: ComponentDefinition
persistent identity: BBa J23118
display id: BBa_J23118
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: constitutive promoter family member
creator: John Anderson
created: 2006-08-16T11:00:00Z
modified: 2015-08-31T04:08:40Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: Later
mutable notes: N/A
mutable provenance: Later
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J23118_sequence/1

BBa_I742145
type: ComponentDefinition
persistent identity: BBa I742145
display id: BBa_I742145
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: sam5 native rbs
creator: Chris French
created: 2007-10-23T11:00:00Z
modified: 2015-08-31T04:08:03Z
discontinued: false
dominant: false
experience: experience/None
group u list: _123_
m user id: 0
owner id: 837
owning group id: 163
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is the native ribosome binding site for sam5 (4-coumarate-3-hydroxylase) of the bacterium Saccharothrix espanaensis.
mutable notes: This is a virtual part to be added to coding sequence I742141.
mutable provenance: Saccharothrix espanaensis DSM 44229
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: rbs
sequence: https://synbiohub.org/public/igem/BBa_I742145_sequence/1

rbs
type: SequenceAnnotation
persistent identity: annotation1955475
display id: annotation1955475
version: 1
top level: BBa_I742145
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_I742145/annotation1955475/range1955475/1

https://synbiohub.org/public/igem/BBa_I742145/annotation1955475/range1955475/1
type: Range
persistent identity: range1955475
display id: range1955475
version: 1
direction: direction/forward
top level: BBa_I742145
start: 3
end: 9
orientation: inline

BBa_J45504
type: ComponentDefinition
persistent identity: BBa J45504
display id: BBa_J45504
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: htpG Heat Shock Promoter
creator: Stephen Payne
created: 2006-06-20T11:00:00Z
modified: 2015-08-31T04:08:49Z
discontinued: false
dominant: false
group u list: _84_
m user id: 0
owner id: 642
owning group id: 84
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This promoter is active at higher temperatures.
mutable notes: None.
mutable provenance: Chris Voigt Lab at UCSF
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J45504_sequence/1

BBa_J34814
type: ComponentDefinition
persistent identity: BBa J34814
display id: BBa_J34814
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: T7 Promoter
creator: Alexandra Choutko
created: 2006-10-25T11:00:00Z
modified: 2015-08-31T04:08:47Z
discontinued: false
dominant: false
experience: experience/None
group u list: _62_
m user id: 0
owner id: 1085
owning group id: 62
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: to be used qith t7 polymerase BBa_J34811
mutable notes: a
mutable provenance: a
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J34814_sequence/1

Bla
type: ComponentDefinition
persistent identity: BBa I14018
display id: BBa_I14018
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: P(Bla)
creator: Vikram Vijayan, Allen Hsu, Lawrence Fomundam
created: 2004-08-01T11:00:00Z
modified: 2015-08-31T04:07:37Z
discontinued: false
dominant: false
experience: experience/None
group u list: _4_
m user id: 0
owner id: 171
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: P(Bla), Medium Transcription
mutable provenance: Plasmid pUC19
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: P(Bla)
sequence annotation: -35
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_I14018_sequence/1

P(Bla)
type: SequenceAnnotation
persistent identity: annotation1018027
display id: annotation1018027
version: 1
top level: Bla
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_I14018/annotation1018027/range1018027/1

https://synbiohub.org/public/igem/BBa_I14018/annotation1018027/range1018027/1
type: Range
persistent identity: range1018027
display id: range1018027
version: 1
direction: direction/forward
top level: Bla
start: 1
end: 35
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1027020
display id: annotation1027020
version: 1
top level: Bla
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I14018/annotation1027020/range1027020/1

https://synbiohub.org/public/igem/BBa_I14018/annotation1027020/range1027020/1
type: Range
persistent identity: range1027020
display id: range1027020
version: 1
direction: direction/forward
top level: Bla
start: 4
end: 9
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1027016
display id: annotation1027016
version: 1
top level: Bla
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I14018/annotation1027016/range1027016/1

https://synbiohub.org/public/igem/BBa_I14018/annotation1027016/range1027016/1
type: Range
persistent identity: range1027016
display id: range1027016
version: 1
direction: direction/forward
top level: Bla
start: 25
end: 30
orientation: inline

BBa_K823014
type: ComponentDefinition
persistent identity: BBa K823014
display id: BBa_K823014
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Anderson promoter J23118
creator: Korinna Kraft
created: 2012-09-07T11:00:00Z
modified: 2015-07-07T11:51:43Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1081_
m user id: 4206
owner id: 12081
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Anderson promoter J23118 without RFP in backbone vector pSB1C3 to easily fuse the promoter with other reporters e.g. the lux operon.
mutable notes: no considerations
mutable provenance: Partsregistry
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: J23118
sequence annotation: -10 box
sequence annotation: -35 box
sequence: https://synbiohub.org/public/igem/BBa_K823014_sequence/1

J23118
type: SequenceAnnotation
persistent identity: annotation2182546
display id: annotation2182546
version: 1
top level: BBa_K823014
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K823014/annotation2182546/range2182546/1

https://synbiohub.org/public/igem/BBa_K823014/annotation2182546/range2182546/1
type: Range
persistent identity: range2182546
display id: range2182546
version: 1
direction: direction/forward
top level: BBa_K823014
start: 1
end: 35
orientation: inline

-10 box
type: SequenceAnnotation
persistent identity: annotation2182548
display id: annotation2182548
version: 1
top level: BBa_K823014
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K823014/annotation2182548/range2182548/1

https://synbiohub.org/public/igem/BBa_K823014/annotation2182548/range2182548/1
type: Range
persistent identity: range2182548
display id: range2182548
version: 1
direction: direction/forward
top level: BBa_K823014
start: 24
end: 29
orientation: inline

-35 box
type: SequenceAnnotation
persistent identity: annotation2182547
display id: annotation2182547
version: 1
top level: BBa_K823014
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K823014/annotation2182547/range2182547/1

https://synbiohub.org/public/igem/BBa_K823014/annotation2182547/range2182547/1
type: Range
persistent identity: range2182547
display id: range2182547
version: 1
direction: direction/forward
top level: BBa_K823014
start: 1
end: 6
orientation: inline

BBa_K090506
type: ComponentDefinition
persistent identity: BBa K090506
display id: BBa_K090506
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: ''Bacillus subtilis'' weak RBS
creator: Daniel Goodman
created: 2008-10-27T12:00:00Z
modified: 2015-05-08T01:08:37Z
discontinued: false
dominant: false
experience: experience/None
group u list: _188_
m user id: 0
owner id: 3501
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is the weak version of the consensus RBS for Bacillus subtilis. The sequence is present in front of some B. subtilis operons, but is expected to give weaker expression than the consensus sequence. The consensus sequence AAAGGAGG (BBa_K090505) was changed to AGAGGTGG for this part.
mutable notes: The part must be 8 nucleotides away from the first methionine of the coding sequence, and so we added 'TGT' downstream of the actual binding sequence: AGAGGTGG. This, combined with the truncated non-CDS BioBrick prefix scar, gives exactly 8 'spacer bases' between the 3' end of the RBS and the start of the coding sequence.
mutable provenance: This part was synthesized directly.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: B. subtilis weak RBS
sequence: https://synbiohub.org/public/igem/BBa_K090506_sequence/1

B. subtilis weak RBS
type: SequenceAnnotation
persistent identity: annotation1992815
display id: annotation1992815
version: 1
top level: BBa_K090506
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K090506/annotation1992815/range1992815/1

https://synbiohub.org/public/igem/BBa_K090506/annotation1992815/range1992815/1
type: Range
persistent identity: range1992815
display id: range1992815
version: 1
direction: direction/forward
top level: BBa_K090506
start: 1
end: 8
orientation: inline

BBa_J56015
type: ComponentDefinition
persistent identity: BBa J56015
display id: BBa_J56015
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: lacIQ - promoter sequence
creator: Matt Eames
created: 2006-10-31T12:00:00Z
modified: 2015-08-31T03:22:59Z
discontinued: false
dominant: false
group u list: _79_98_
m user id: 0
owner id: 1132
owning group id: 98
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Promoter sequence for constituitive expression
mutable notes: None
mutable provenance: Synthesized by DNA 2.0
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J56015_sequence/1

BBa_B2101
type: ComponentDefinition
persistent identity: BBa B2101
display id: BBa_B2101
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: T7 RBS 0.3 + SapI (rev)
creator: Heather Keller
created: 2006-09-27T11:00:00Z
modified: 2015-08-31T04:07:21Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_
m user id: 0
owner id: 571
owning group id: 10
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This version of the T7 0.3 RBS is designed for construction via Heather Keller's alternative assembly plan using XbaI and SapI for RBS/CDS assembly without a mixed site. Contains the -20 to -1 region of gp0.4 from the wild-type T7 genome. Can also be used with Standard BioBricks assembly, although this will add a 7 amino acid fusion to the N-terminus of the resulting protein.
mutable notes: This RBS is designed to be compatible with Heather Keller's RBS assembly scheme for creating RBS/CDS junctions without a mixed site. For use in this scheme, this part should be cut with XbaI and SapI. It must be used with a coding sequence containing the SapI site at the 5' end. This part is not compatible with CDSs that use a GTG site and must be used with CDSs using an ATG start site. The two bp spacer on the 3' end also allows this part to be used in standard biobricks assembly and is necessary to maintain the reading frame of a downstream CDS. Note that Standard Biobricks Assembly with this part results in the creation of a new ATG start site, and a 7 amino acid fusion at the N terminus of the downstream protein. CDSs with alternative start sites are compatible with this part via Standard Assembly. This part was inadvertently constructed without the single base pair (G/C) that separates the XbaI site and the 5' end of the part.
mutable provenance: The RBS is taken from the wild-type bacteriophage T7 genome. Please see part B2001 for more information.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/DNA
role: SO:0000110
component: BBa_B2001
sequence annotation: BBa_B2001
sequence: https://synbiohub.org/public/igem/BBa_B2101_sequence/1

BBa_B2001
type: Component
persistent identity: component2218355
display id: component2218355
version: 1
top level: BBa_B2101
access: public
definition: T7 0.3

BBa_B2001
type: SequenceAnnotation
persistent identity: annotation2218355
display id: annotation2218355
version: 1
top level: BBa_B2101
location: https://synbiohub.org/public/igem/BBa_B2101/annotation2218355/range2218355/1
component: BBa_B2001

https://synbiohub.org/public/igem/BBa_B2101/annotation2218355/range2218355/1
type: Range
persistent identity: range2218355
display id: range2218355
version: 1
direction: direction/reverse
top level: BBa_B2101
start: 1
end: 20
orientation: inline

BBa_K1468000
type: ComponentDefinition
persistent identity: BBa K1468000
display id: BBa_K1468000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pJ23104 + gene encoding ZsGreen
creator: Pedro Luis Dorado Morales
created: 2014-10-02T11:00:00Z
modified: 2015-05-08T01:10:38Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1847_
m user id: 0
owner id: 11756
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: -
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: ZsGreen protein
sequence annotation: STOP
sequence annotation: BBa_K1468000
sequence annotation: BBa_J23104
sequence annotation: ZsGreeen
sequence annotation: START
sequence annotation: BBa_E0040
sequence annotation: J23104p
sequence: https://synbiohub.org/public/igem/BBa_K1468000_sequence/1

ZsGreen protein
type: SequenceAnnotation
persistent identity: annotation2418059
display id: annotation2418059
version: 1
top level: BBa_K1468000
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1468000/annotation2418059/range2418059/1

https://synbiohub.org/public/igem/BBa_K1468000/annotation2418059/range2418059/1
type: Range
persistent identity: range2418059
display id: range2418059
version: 1
direction: direction/forward
top level: BBa_K1468000
start: 34
end: 725
orientation: inline

STOP
type: SequenceAnnotation
persistent identity: annotation2418064
display id: annotation2418064
version: 1
top level: BBa_K1468000
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1468000/annotation2418064/range2418064/1

https://synbiohub.org/public/igem/BBa_K1468000/annotation2418064/range2418064/1
type: Range
persistent identity: range2418064
display id: range2418064
version: 1
direction: direction/forward
top level: BBa_K1468000
start: 725
end: 725
orientation: inline

BBa_K1468000
type: SequenceAnnotation
persistent identity: annotation2418810
display id: annotation2418810
version: 1
top level: BBa_K1468000
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1468000/annotation2418810/range2418810/1

https://synbiohub.org/public/igem/BBa_K1468000/annotation2418810/range2418810/1
type: Range
persistent identity: range2418810
display id: range2418810
version: 1
direction: direction/forward
top level: BBa_K1468000
start: 1
end: 725
orientation: inline

BBa_J23104
type: SequenceAnnotation
persistent identity: annotation2418058
display id: annotation2418058
version: 1
top level: BBa_K1468000
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1468000/annotation2418058/range2418058/1

https://synbiohub.org/public/igem/BBa_K1468000/annotation2418058/range2418058/1
type: Range
persistent identity: range2418058
display id: range2418058
version: 1
direction: direction/forward
top level: BBa_K1468000
start: 1
end: 33
orientation: inline

ZsGreeen
type: SequenceAnnotation
persistent identity: annotation2418062
display id: annotation2418062
version: 1
top level: BBa_K1468000
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1468000/annotation2418062/range2418062/1

https://synbiohub.org/public/igem/BBa_K1468000/annotation2418062/range2418062/1
type: Range
persistent identity: range2418062
display id: range2418062
version: 1
direction: direction/forward
top level: BBa_K1468000
start: 34
end: 725
orientation: inline

START
type: SequenceAnnotation
persistent identity: annotation2418061
display id: annotation2418061
version: 1
top level: BBa_K1468000
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1468000/annotation2418061/range2418061/1

https://synbiohub.org/public/igem/BBa_K1468000/annotation2418061/range2418061/1
type: Range
persistent identity: range2418061
display id: range2418061
version: 1
direction: direction/forward
top level: BBa_K1468000
start: 34
end: 36
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation2418060
display id: annotation2418060
version: 1
top level: BBa_K1468000
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1468000/annotation2418060/range2418060/1

https://synbiohub.org/public/igem/BBa_K1468000/annotation2418060/range2418060/1
type: Range
persistent identity: range2418060
display id: range2418060
version: 1
direction: direction/forward
top level: BBa_K1468000
start: 34
end: 725
orientation: inline

J23104p
type: SequenceAnnotation
persistent identity: annotation2418030
display id: annotation2418030
version: 1
top level: BBa_K1468000
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1468000/annotation2418030/range2418030/1

https://synbiohub.org/public/igem/BBa_K1468000/annotation2418030/range2418030/1
type: Range
persistent identity: range2418030
display id: range2418030
version: 1
direction: direction/forward
top level: BBa_K1468000
start: 1
end: 33
orientation: inline

Pveg
type: ComponentDefinition
persistent identity: BBa K143012
display id: BBa_K143012
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter veg a constitutive promoter for B. subtilis
creator: James Chappell
created: 2008-09-10T11:00:00Z
modified: 2015-05-08T01:10:23Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _199_
m user id: 0
owner id: 2090
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Pveg is a constitutive promoter that constitutively expresses the P43 protein in ''B.subtilis''. Pveg contains binding sites for the ''B.sutbilis'' major sigma factor#1. Pveg in ''B.subtilis'' utilises two binding sites to cause high expression of genes#2, however our Pveg is lacking the upstream site to give a medium level of gene expression. It has been noted that the sporulation master regulatoion factor spoOA interacts with Pveg though it is not known how#3. The context with which we used the promoter Pveg is as a '''Polymerase Per Second''' (PoPS) generator.
mutable notes: The biobrick part was designed to include a single binding site for the ''B.subtilis major sigma factor. In addition the biobrick standard was applied to the promoter Pveg sequence.
mutable provenance: The Pveg promoter was suggested to us by Dr. Jan-Willem Veening of Newcastle University. This sequence supplied was compared to that of the DBTBS database#3 then a section containing the binding site synthesised by Geneart.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: Sigma A-35
sequence annotation: Sigma A -10
sequence: https://synbiohub.org/public/igem/BBa_K143012_sequence/1

Sigma A-35
type: SequenceAnnotation
persistent identity: annotation1975704
display id: annotation1975704
version: 1
top level: Pveg
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K143012/annotation1975704/range1975704/1

https://synbiohub.org/public/igem/BBa_K143012/annotation1975704/range1975704/1
type: Range
persistent identity: range1975704
display id: range1975704
version: 1
direction: direction/forward
top level: Pveg
start: 63
end: 68
orientation: inline

Sigma A -10
type: SequenceAnnotation
persistent identity: annotation1975705
display id: annotation1975705
version: 1
top level: Pveg
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K143012/annotation1975705/range1975705/1

https://synbiohub.org/public/igem/BBa_K143012/annotation1975705/range1975705/1
type: Range
persistent identity: range1975705
display id: range1975705
version: 1
direction: direction/forward
top level: Pveg
start: 86
end: 91
orientation: inline

BBa_J23112
type: ComponentDefinition
persistent identity: BBa J23112
display id: BBa_J23112
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: constitutive promoter family member
creator: John Anderson
created: 2006-08-16T11:00:00Z
modified: 2015-08-31T04:08:40Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: Later
mutable notes: N/A
mutable provenance: Later
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J23112_sequence/1

BBa_K382022
type: ComponentDefinition
persistent identity: BBa K382022
display id: BBa_K382022
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pENTCUP2 plant specific promoter
creator: Patrick Boyle
created: 2010-10-25T11:00:00Z
modified: 2015-06-11T01:13:46Z
discontinued: false
dominant: false
group u list: _488_
m user id: 4206
owner id: 7814
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: pENTCUP2 promoter for the constitutive expression of genes in plants.
mutable notes: Amplified from PCR from the pORE E3 plasmid.
mutable provenance: Derived from the pORE vector series (see http://2010.igem.org/Team:Harvard).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K382022_sequence/1

BBa_K823000
type: ComponentDefinition
persistent identity: BBa K823000
display id: BBa_K823000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PliaG
creator: Korinna Kraft
created: 2012-07-15T11:00:00Z
modified: 2015-05-08T01:13:29Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1081_
m user id: 0
owner id: 12081
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Promoter of the liaG gene of Bacillus subtilis, weak constitutive
mutable notes: no considerations
mutable provenance: Bacillus subtilis
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -10 box
sequence annotation: PliaG
sequence annotation: -35 box
sequence: https://synbiohub.org/public/igem/BBa_K823000_sequence/1

-10 box
type: SequenceAnnotation
persistent identity: annotation2182555
display id: annotation2182555
version: 1
top level: BBa_K823000
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K823000/annotation2182555/range2182555/1

https://synbiohub.org/public/igem/BBa_K823000/annotation2182555/range2182555/1
type: Range
persistent identity: range2182555
display id: range2182555
version: 1
direction: direction/forward
top level: BBa_K823000
start: 105
end: 110
orientation: inline

PliaG
type: SequenceAnnotation
persistent identity: annotation2182553
display id: annotation2182553
version: 1
top level: BBa_K823000
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K823000/annotation2182553/range2182553/1

https://synbiohub.org/public/igem/BBa_K823000/annotation2182553/range2182553/1
type: Range
persistent identity: range2182553
display id: range2182553
version: 1
direction: direction/forward
top level: BBa_K823000
start: 1
end: 121
orientation: inline

-35 box
type: SequenceAnnotation
persistent identity: annotation2182554
display id: annotation2182554
version: 1
top level: BBa_K823000
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K823000/annotation2182554/range2182554/1

https://synbiohub.org/public/igem/BBa_K823000/annotation2182554/range2182554/1
type: Range
persistent identity: range2182554
display id: range2182554
version: 1
direction: direction/forward
top level: BBa_K823000
start: 81
end: 86
orientation: inline

BBa_K137091
type: ComponentDefinition
persistent identity: BBa K137091
display id: BBa_K137091
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: optimized (A) repeat constitutive promoter with 18 bp between -10 and -35 elements
creator: Allen Lin
created: 2008-08-20T11:00:00Z
modified: 2015-05-08T01:10:10Z
discontinued: false
dominant: false
experience: experience/None
group u list: _187_
m user id: 0
owner id: 3112
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: optimized (A) repeat constitutive promoter with 18 bp between -10 and -35 elements
mutable notes: None
mutable provenance: PCR
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K137091_sequence/1

BBa_J23110
type: ComponentDefinition
persistent identity: BBa J23110
display id: BBa_J23110
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: constitutive promoter family member
creator: John Anderson
created: 2006-08-16T11:00:00Z
modified: 2015-08-31T04:08:40Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: true
mutable description: Later
mutable notes: N/A
mutable provenance: Later
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J23110_sequence/1

BBa_M13511
type: ComponentDefinition
persistent identity: BBa M13511
display id: BBa_M13511
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: M13K07 gene XI RBS
creator: Natalie Kuldell
created: 2006-12-14T12:00:00Z
modified: 2015-05-08T01:13:56Z
discontinued: false
dominant: false
experience: experience/None
group u list: _45_
m user id: 0
owner id: 314
owning group id: 1
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This RBS is part of the bacteriophage M13 genome, initiating translation of the gene XI message (BBa_M13011). The part aligns with base pairs 3900-3915 in M13K07 genome. It was NOT identified as the RBS for gene XI message by Wezenbeek et al in Gene (1980) 11: 129-148
mutable notes: none
mutable provenance: New England Biolabs M13K07. Sequenced in 2002 by L. Panganaban
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_M13511_sequence/1

creA RBS
type: ComponentDefinition
persistent identity: BBa J64907
display id: BBa_J64907
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: creA RBS
creator: Ryan Ritterson
created: 2007-03-19T12:00:00Z
modified: 2015-05-08T01:08:17Z
discontinued: false
dominant: false
experience: experience/None
group u list: _98_
m user id: 0
owner id: 1422
owning group id: 98
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: ribosome binding site for creA gene in the E Coli cre operon
mutable notes: natural sequence, no design.
mutable provenance: E Coli strain K12
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J64907_sequence/1

BBa_K823008
type: ComponentDefinition
persistent identity: BBa K823008
display id: BBa_K823008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Anderson promoter J23106
creator: Korinna Kraft
created: 2012-09-07T11:00:00Z
modified: 2015-07-07T11:47:05Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1081_
m user id: 4206
owner id: 12081
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Anderson promoter J23106 without RFP in backbone vector pSB1C3 to easily fuse the promoter with other reporters e.g. the lux operon.
mutable notes: no considerations
mutable provenance: Partsregistry
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -35 box
sequence annotation: -10 box
sequence annotation: J23106
sequence: https://synbiohub.org/public/igem/BBa_K823008_sequence/1

-35 box
type: SequenceAnnotation
persistent identity: annotation2182528
display id: annotation2182528
version: 1
top level: BBa_K823008
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K823008/annotation2182528/range2182528/1

https://synbiohub.org/public/igem/BBa_K823008/annotation2182528/range2182528/1
type: Range
persistent identity: range2182528
display id: range2182528
version: 1
direction: direction/forward
top level: BBa_K823008
start: 1
end: 6
orientation: inline

-10 box
type: SequenceAnnotation
persistent identity: annotation2182529
display id: annotation2182529
version: 1
top level: BBa_K823008
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K823008/annotation2182529/range2182529/1

https://synbiohub.org/public/igem/BBa_K823008/annotation2182529/range2182529/1
type: Range
persistent identity: range2182529
display id: range2182529
version: 1
direction: direction/forward
top level: BBa_K823008
start: 24
end: 29
orientation: inline

J23106
type: SequenceAnnotation
persistent identity: annotation2182530
display id: annotation2182530
version: 1
top level: BBa_K823008
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K823008/annotation2182530/range2182530/1

https://synbiohub.org/public/igem/BBa_K823008/annotation2182530/range2182530/1
type: Range
persistent identity: range2182530
display id: range2182530
version: 1
direction: direction/forward
top level: BBa_K823008
start: 1
end: 35
orientation: inline

BBa_J23116
type: ComponentDefinition
persistent identity: BBa J23116
display id: BBa_J23116
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: constitutive promoter family member
creator: John Anderson
created: 2006-08-16T11:00:00Z
modified: 2015-08-31T04:08:40Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: true
mutable description: Later
mutable notes: N/A
mutable provenance: Later
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J23116_sequence/1

BBa_J61128
type: ComponentDefinition
persistent identity: BBa J61128
display id: BBa_J61128
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ribosome Binding Site Family Member
creator: John Anderson
created: 2007-04-24T11:00:00Z
modified: 2015-08-31T01:59:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable description: {{JCA_Arkin_RBSFamily}}
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J61128_sequence/1

BBa_B0038
type: ComponentDefinition
persistent identity: BBa B0038
display id: BBa_B0038
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS 1
creator: Josh Michener
created: 2005-12-20T12:00:00Z
modified: 2015-08-31T04:07:20Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_
m user id: 0
owner id: 253
owning group id: 6
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Modified version of B0031 from the Voigt Lab. Not currently available as an independent part (only in a tet inverter).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_B0038_sequence/1

BBa_K1585101
type: ComponentDefinition
persistent identity: BBa K1585101
display id: BBa_K1585101
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Anderson Promoter with lacI binding site
creator: Tobias Schwanemann
created: 2015-08-23T11:00:00Z
modified: 2015-09-01T03:27:19Z
discontinued: false
dominant: false
experience: experience/Fails
group u list: _2002_
m user id: 24474
owner id: 24474
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Promoters of the Anderson Promoter Collection with lacI binding site derived from [http://parts.igem.org/Part:BBa_R0010 R_0010] to make them [http://openwetware.org/wiki/IPTG IPTG] iducible.
mutable notes: The "lacI binding site" of Part [http://parts.igem.org/Part:BBa_R0010 R_0010] differs from lacO from Part [http://parts.igem.org/Part:BBa_J33207 BBa_J33207] although they should be same. We choosed part BBa_R0010 because we assumed older parts would be reliable.
mutable provenance: Assambly of [http://parts.igem.org/Part:BBa_J23100 J23100] and lacI-binding-site of [http://parts.igem.org/Part:BBa_R0010 R_0010]
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
component: BBa_J23101
component: BBa_K1585999
sequence annotation: BBa_J23101
sequence annotation: BBa_K1585999
sequence: https://synbiohub.org/public/igem/BBa_K1585101_sequence/1

BBa_J23101
type: Component
persistent identity: component2436906
display id: component2436906
version: 1
top level: BBa_K1585101
access: public
definition: BBa_J23101

BBa_K1585999
type: Component
persistent identity: component2436907
display id: component2436907
version: 1
top level: BBa_K1585101
access: public
definition: https://synbiohub.org/public/igem/BBa_K1585999/1

BBa_J23101
type: SequenceAnnotation
persistent identity: annotation2436906
display id: annotation2436906
version: 1
top level: BBa_K1585101
location: https://synbiohub.org/public/igem/BBa_K1585101/annotation2436906/range2436906/1
component: BBa_J23101

https://synbiohub.org/public/igem/BBa_K1585101/annotation2436906/range2436906/1
type: Range
persistent identity: range2436906
display id: range2436906
version: 1
direction: direction/reverse
top level: BBa_K1585101
start: 1
end: 35
orientation: inline

BBa_K1585999
type: SequenceAnnotation
persistent identity: annotation2436907
display id: annotation2436907
version: 1
top level: BBa_K1585101
location: https://synbiohub.org/public/igem/BBa_K1585101/annotation2436907/range2436907/1
component: BBa_K1585999

https://synbiohub.org/public/igem/BBa_K1585101/annotation2436907/range2436907/1
type: Range
persistent identity: range2436907
display id: range2436907
version: 1
direction: direction/reverse
top level: BBa_K1585101
start: 44
end: 78
orientation: inline

BBa_B2022
type: ComponentDefinition
persistent identity: BBa B2022
display id: BBa_B2022
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: 4B RBS from T7
creator: Bartholomew Canton
created: 2007-05-22T11:00:00Z
modified: 2015-08-31T04:07:21Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_
m user id: 0
owner id: 135
owning group id: 84
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: to be added automatically later
mutable notes: to be added automatically later
mutable provenance: to be added automatically later
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_B2022_sequence/1

BBa_R0183
type: ComponentDefinition
persistent identity: BBa R0183
display id: BBa_R0183
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: T7 RNAP promoter
creator: Barry Canton
created: 2005-09-25T11:00:00Z
modified: 2015-05-08T01:14:15Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_6_
m user id: 0
owner id: 135
owning group id: 6
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: T7 promoter with an A->C mutation at the -16 position of the consensus sequence
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_R0183_sequence/1

BBa_K105031
type: ComponentDefinition
persistent identity: BBa K105031
display id: BBa_K105031
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: cyc16 minimal promoter
creator: Michael Wild
created: 2008-10-25T11:00:00Z
modified: 2015-05-08T01:08:52Z
discontinued: false
dominant: false
experience: experience/None
group u list: _253_
m user id: 0
owner id: 3313
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This is a derivate of BBa_K105027. Due to a point mutation in the TATA box region the efficiency of this minimal promoter is only 16 % of the natural yeast CYC1 promoter.
mutable notes: This BioBrick contains the TATA-box with one point mutation and most of the 5'UTR (-139 to -36, relative to the translational start) of the natural CYC1 promoter. A Kozak sequence is not included and has thus to be added! Even if there isn't any need for this BioBrick to be fusible to other protein domains, we stayed with our design.
mutable provenance: Amplification and Mutation from the plasmid pAH3
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: TATA-box
sequence annotation: A -> G
sequence: https://synbiohub.org/public/igem/BBa_K105031_sequence/1

TATA-box
type: SequenceAnnotation
persistent identity: annotation1989843
display id: annotation1989843
version: 1
top level: BBa_K105031
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K105031/annotation1989843/range1989843/1

https://synbiohub.org/public/igem/BBa_K105031/annotation1989843/range1989843/1
type: Range
persistent identity: range1989843
display id: range1989843
version: 1
direction: direction/forward
top level: BBa_K105031
start: 17
end: 23
orientation: inline

A -> G
type: SequenceAnnotation
persistent identity: annotation1989845
display id: annotation1989845
version: 1
top level: BBa_K105031
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_K105031/annotation1989845/range1989845/1

https://synbiohub.org/public/igem/BBa_K105031/annotation1989845/range1989845/1
type: Range
persistent identity: range1989845
display id: range1989845
version: 1
direction: direction/forward
top level: BBa_K105031
start: 22
end: 22
orientation: inline

BBa_M31519
type: ComponentDefinition
persistent identity: BBa M31519
display id: BBa_M31519
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Modified promoter sequence of g3.
creator: Han Zhu
created: 2007-02-27T12:00:00Z
modified: 2015-05-08T01:14:00Z
discontinued: false
dominant: false
group u list: _102_
m user id: 0
owner id: 1377
owning group id: 102
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is the promoter sequence of g3 which has been separated from g8. It also includes the restriction enzyme EcoRV in front.
mutable notes: This part used in conjunction with BBa_M131520 allows g8 and promoter of g3 to be separate from each other, therefore allowing free regulation of g3 with no consequences on g8.
mutable provenance: GATATCaattcacctcgaaagcaagctgataaaccgatacaattaaaggctcct
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: EcoRV
sequence annotation: promoter-g3
sequence annotation: BmtI
sequence: https://synbiohub.org/public/igem/BBa_M31519_sequence/1

EcoRV
type: SequenceAnnotation
persistent identity: annotation1915217
display id: annotation1915217
version: 1
top level: BBa_M31519
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_M31519/annotation1915217/range1915217/1

https://synbiohub.org/public/igem/BBa_M31519/annotation1915217/range1915217/1
type: Range
persistent identity: range1915217
display id: range1915217
version: 1
direction: direction/forward
top level: BBa_M31519
start: 1
end: 6
orientation: inline

promoter-g3
type: SequenceAnnotation
persistent identity: annotation1915216
display id: annotation1915216
version: 1
top level: BBa_M31519
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_M31519/annotation1915216/range1915216/1

https://synbiohub.org/public/igem/BBa_M31519/annotation1915216/range1915216/1
type: Range
persistent identity: range1915216
display id: range1915216
version: 1
direction: direction/forward
top level: BBa_M31519
start: 7
end: 54
orientation: inline

BmtI
type: SequenceAnnotation
persistent identity: annotation1919691
display id: annotation1919691
version: 1
top level: BBa_M31519
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_M31519/annotation1919691/range1919691/1

https://synbiohub.org/public/igem/BBa_M31519/annotation1919691/range1919691/1
type: Range
persistent identity: range1919691
display id: range1919691
version: 1
direction: direction/forward
top level: BBa_M31519
start: 54
end: 60
orientation: inline

BBa_K105030
type: ComponentDefinition
persistent identity: BBa K105030
display id: BBa_K105030
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: cyc28 minimal promoter
creator: Michael Wild
created: 2008-10-25T11:00:00Z
modified: 2015-05-08T01:08:52Z
discontinued: false
dominant: false
experience: experience/None
group u list: _253_
m user id: 0
owner id: 3313
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This is a derivate of BBa_K105027. Due to a point mutation in the TATA box region the efficiency of this minimal promoter is only 28 % of the natural yeast CYC1 promoter.
mutable notes: This BioBrick contains the TATA-box with one point mutation and most of the 5'UTR (-139 to -36, relative to the translational start) of the natural CYC1 promoter. A Kozak sequence is not included and has thus to be added! Even if there isn't any need for this BioBrick to be fusible to other protein domains, we stayed with our design.
mutable provenance: Amplification and Mutation from the plasmid pAH3
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: TATA-box
sequence annotation: A -> T
sequence: https://synbiohub.org/public/igem/BBa_K105030_sequence/1

TATA-box
type: SequenceAnnotation
persistent identity: annotation1989839
display id: annotation1989839
version: 1
top level: BBa_K105030
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K105030/annotation1989839/range1989839/1

https://synbiohub.org/public/igem/BBa_K105030/annotation1989839/range1989839/1
type: Range
persistent identity: range1989839
display id: range1989839
version: 1
direction: direction/forward
top level: BBa_K105030
start: 17
end: 23
orientation: inline

A -> T
type: SequenceAnnotation
persistent identity: annotation1989840
display id: annotation1989840
version: 1
top level: BBa_K105030
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_K105030/annotation1989840/range1989840/1

https://synbiohub.org/public/igem/BBa_K105030/annotation1989840/range1989840/1
type: Range
persistent identity: range1989840
display id: range1989840
version: 1
direction: direction/forward
top level: BBa_K105030
start: 22
end: 22
orientation: inline

P0310
type: ComponentDefinition
persistent identity: BBa J63005
display id: BBa_J63005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: yeast ADH1 promoter
creator: Caroline Ajo-Franklin
created: 2006-10-10T11:00:00Z
modified: 2015-08-31T01:56:26Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _97_
m user id: 0
owner id: 545
owning group id: 97
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: ADH1 promoter from S. cerevisiae
mutable notes: no changes to genomic sequence
mutable provenance: genomic DNA of ADH1 from S. cerevisiae
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: Constitutive promoter
sequence annotation: TATA Box
sequence: https://synbiohub.org/public/igem/BBa_J63005_sequence/1

Constitutive promoter
type: SequenceAnnotation
persistent identity: annotation1902744
display id: annotation1902744
version: 1
top level: P0310
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J63005/annotation1902744/range1902744/1

https://synbiohub.org/public/igem/BBa_J63005/annotation1902744/range1902744/1
type: Range
persistent identity: range1902744
display id: range1902744
version: 1
direction: direction/forward
top level: P0310
start: 1
end: 1445
orientation: inline

TATA Box
type: SequenceAnnotation
persistent identity: annotation1902745
display id: annotation1902745
version: 1
top level: P0310
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_J63005/annotation1902745/range1902745/1

https://synbiohub.org/public/igem/BBa_J63005/annotation1902745/range1902745/1
type: Range
persistent identity: range1902745
display id: range1902745
version: 1
direction: direction/forward
top level: P0310
start: 1348
end: 1354
orientation: inline

BBa_K1468001
type: ComponentDefinition
persistent identity: BBa K1468001
display id: BBa_K1468001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pJ23104 + gene encoding AsRed2
creator: Pedro Luis Dorado Morales
created: 2014-10-02T11:00:00Z
modified: 2015-05-08T01:10:38Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1847_
m user id: 0
owner id: 11756
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: -
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence: https://synbiohub.org/public/igem/BBa_K1468001_sequence/1

BBa_B2040
type: ComponentDefinition
persistent identity: BBa B2040
display id: BBa_B2040
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: 8 RBS from T7
creator: Bartholomew Canton
created: 2007-05-22T11:00:00Z
modified: 2015-08-31T04:07:21Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_
m user id: 0
owner id: 135
owning group id: 84
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: to be added automatically later
mutable notes: to be added automatically later
mutable provenance: to be added automatically later
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_B2040_sequence/1

BBa_K088007
type: ComponentDefinition
persistent identity: BBa K088007
display id: BBa_K088007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: GlnRS promoter
creator: Yaguang Liu
created: 2008-10-23T11:00:00Z
modified: 2015-05-08T01:08:37Z
discontinued: false
dominant: false
group u list: _190_
m user id: 0
owner id: 3108
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part promotes the transcription of TyrRS(aminoacyl tRNA synthetase).
mutable notes: It is a weak promoter.
mutable provenance: E.coli We synthetized it according to the GeneBank.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K088007_sequence/1

KP3
type: ComponentDefinition
persistent identity: BBa J01006
display id: BBa_J01006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Key Promoter absorbs 3
creator: Golden Bear
created: 2005-10-31T12:00:00Z
modified: 2015-08-31T04:08:11Z
discontinued: false
dominant: false
group u list: _13_
m user id: 0
owner id: 395
owning group id: 13
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Promoter for transcribing keys to act on locks based on Isaacs, Collins, et. al: "Engineered riboregulators enable post-transcriptional control of gene expression" KP3 absorbs three nucleotides from mixed site ("TACTAGAG"), so that the key has a 5 nucleotide spacer region (i.e. "TAGAG") between the transcription start site and the first nucleotide of the key. We are still looking into what the optimal size for the spacer region is. See also KP2 (BBa_J01007), and Key1 (BBa_J01008), Key2 (BBa_J01009), Lock1 (BBa_J01010), and Lock2 (BBa_J01011).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J01006_sequence/1

BBa_J23100
type: ComponentDefinition
persistent identity: BBa J23100
display id: BBa_J23100
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: constitutive promoter family member
creator: John Anderson
created: 2006-08-03T11:00:00Z
modified: 2015-08-31T04:08:40Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: Replace later
mutable notes: N/A
mutable provenance: Isolated from library of promoters
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J23100_sequence/1

Pnif
type: ComponentDefinition
persistent identity: BBa K1796001
display id: BBa_K1796001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: promoter of Paenibacillus sp. WLY78 nif gene cluster
creator: Nannan Xie
created: 2015-09-16T11:00:00Z
modified: 2015-09-18T02:35:42Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2221_
m user id: 12637
owner id: 23592
owning group id: 9
status: status/Planning
bookmark: false
mutable description: The promoter of Paenibacillus sp. WLY78 nif gene cluster, We named it Pnif. Pnif is a σ70-dependent promoter located upstream of nifB. Pnif is in control of all the gene clusters of nitrogen fixation genome. Confirmation of nif promoter We standardized the nif promoter of Paenibacillus sp. WLY78, and linked it to the upstream of 3 reporter genes (RFP reporter: BBa_K1357010, YFP reporter: BBa_E0430;amilCP reporter: BBa_K1357009) to confirm the reliability of this promoter. The results are shown in Figure 2. A B C http://2015.igem.org/wiki/images/b/b2/Confirmation_of_Pnif_AB.png http://2015.igem.org/wiki/images/thumb/3/34/C-confirmation_of_Pnif.jpeg/622px-C-confirmation_of_Pnif.jpeg Fig.4. Confirmation of nif promoter of Paenibacillus sp. WLY78. (A) Pnif+RFP reporter; (B) Pnif+YFP reporter;(C)Pnif+amilCP reporter. The results confirmed the reliability of Pnif. The initiation of Pnif does not require inducement. Thus it is a effective constitutive promoter.
mutable notes: effeciency of the promoter in E.coli for ordinary parts
mutable provenance: Pnif is from Paenibacillus sp. WLY78 genome,located upstream of nifB,the first gene of nitrogen fixation cluster.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -35
sequence annotation: -10
sequence annotation: Pnif
sequence: https://synbiohub.org/public/igem/BBa_K1796001_sequence/1

-35
type: SequenceAnnotation
persistent identity: annotation2473637
display id: annotation2473637
version: 1
top level: Pnif
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K1796001/annotation2473637/range2473637/1

https://synbiohub.org/public/igem/BBa_K1796001/annotation2473637/range2473637/1
type: Range
persistent identity: range2473637
display id: range2473637
version: 1
direction: direction/forward
top level: Pnif
start: 62
end: 67
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2473660
display id: annotation2473660
version: 1
top level: Pnif
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K1796001/annotation2473660/range2473660/1

https://synbiohub.org/public/igem/BBa_K1796001/annotation2473660/range2473660/1
type: Range
persistent identity: range2473660
display id: range2473660
version: 1
direction: direction/forward
top level: Pnif
start: 85
end: 90
orientation: inline

Pnif
type: SequenceAnnotation
persistent identity: annotation2473661
display id: annotation2473661
version: 1
top level: Pnif
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1796001/annotation2473661/range2473661/1

https://synbiohub.org/public/igem/BBa_K1796001/annotation2473661/range2473661/1
type: Range
persistent identity: range2473661
display id: range2473661
version: 1
direction: direction/forward
top level: Pnif
start: 1
end: 100
orientation: inline

BBa_J61102
type: ComponentDefinition
persistent identity: BBa J61102
display id: BBa_J61102
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ribosome Binding Site Family Member
creator: John Anderson
created: 2007-01-28T12:00:00Z
modified: 2015-08-31T02:03:00Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable description: {{JCA_Arkin_RBSFamily}}
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J61102_sequence/1

BBa_J64998
type: ComponentDefinition
persistent identity: BBa J64998
display id: BBa_J64998
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: consensus -10 and rest from SP6
creator: Sai Duriseti
created: 2007-03-25T11:00:00Z
modified: 2015-05-08T01:08:18Z
discontinued: false
dominant: false
experience: experience/None
group u list: _98_
m user id: 0
owner id: 1428
owning group id: 98
sample status: Not in stock
status: status/Unavailable
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: Sigma 70 -10
sequence: https://synbiohub.org/public/igem/BBa_J64998_sequence/1

Sigma 70 -10
type: SequenceAnnotation
persistent identity: annotation1921745
display id: annotation1921745
version: 1
top level: BBa_J64998
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J64998/annotation1921745/range1921745/1

https://synbiohub.org/public/igem/BBa_J64998/annotation1921745/range1921745/1
type: Range
persistent identity: range1921745
display id: range1921745
version: 1
direction: direction/forward
top level: BBa_J64998
start: 1
end: 19
orientation: inline

BBa_J64013
type: ComponentDefinition
persistent identity: BBa J64013
display id: BBa_J64013
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: sipA RBS
creator: Dan Widmaier
created: 2008-01-16T12:00:00Z
modified: 2015-08-31T01:56:26Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _98_
m user id: 0
owner id: 2407
owning group id: 98
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is the natural RBS that drives the sipA effector protein in the Salmonella SPI-1 system
mutable notes: This part was not designed with compatibility to biobrick standard assembly in mind. This RBS has not been explicitly tested in an expression assasy such as B-Gal or GFP expression.
mutable provenance: Salmonella LT2 Genomic DNA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: sicA RBS
sequence: https://synbiohub.org/public/igem/BBa_J64013_sequence/1

sicA RBS
type: SequenceAnnotation
persistent identity: annotation1959612
display id: annotation1959612
version: 1
top level: BBa_J64013
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_J64013/annotation1959612/range1959612/1

https://synbiohub.org/public/igem/BBa_J64013/annotation1959612/range1959612/1
type: Range
persistent identity: range1959612
display id: range1959612
version: 1
direction: direction/forward
top level: BBa_J64013
start: 1
end: 18
orientation: inline

BBa_J61125
type: ComponentDefinition
persistent identity: BBa J61125
display id: BBa_J61125
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ribosome Binding Site Family Member
creator: John Anderson
created: 2007-04-24T11:00:00Z
modified: 2015-08-31T01:59:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable description: {{JCA_Arkin_RBSFamily}}
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J61125_sequence/1

BBa_I742163
type: ComponentDefinition
persistent identity: BBa I742163
display id: BBa_I742163
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: native ftsK rbs
creator: Chris French
created: 2007-10-24T11:00:00Z
modified: 2015-08-31T04:08:03Z
discontinued: false
dominant: false
experience: experience/None
group u list: _123_
m user id: 0
owner id: 837
owning group id: 163
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is the native ribosome binding site of ftsK, a DNA-translocating motor protein involved in cell division in Escherichia coli.
mutable notes: Virtual part to be added to coding sequence BBa_I742137.
mutable provenance: Escherichia coli JM109 (a K12 strain).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: rbs
sequence annotation: SacI
sequence: https://synbiohub.org/public/igem/BBa_I742163_sequence/1

rbs
type: SequenceAnnotation
persistent identity: annotation1954641
display id: annotation1954641
version: 1
top level: BBa_I742163
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_I742163/annotation1954641/range1954641/1

https://synbiohub.org/public/igem/BBa_I742163/annotation1954641/range1954641/1
type: Range
persistent identity: range1954641
display id: range1954641
version: 1
direction: direction/forward
top level: BBa_I742163
start: 15
end: 20
orientation: inline

SacI
type: SequenceAnnotation
persistent identity: annotation1954640
display id: annotation1954640
version: 1
top level: BBa_I742163
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I742163/annotation1954640/range1954640/1

https://synbiohub.org/public/igem/BBa_I742163/annotation1954640/range1954640/1
type: Range
persistent identity: range1954640
display id: range1954640
version: 1
direction: direction/forward
top level: BBa_I742163
start: 1
end: 3
orientation: inline

BBa_J23119
type: ComponentDefinition
persistent identity: BBa J23119
display id: BBa_J23119
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: constitutive promoter family member
creator: John Anderson
created: 2006-08-23T11:00:00Z
modified: 2015-08-31T04:08:40Z
discontinued: false
dominant: true
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Later
mutable notes: N/A
mutable provenance: Overlap extension of synthetic oligonucleotides
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J23119_sequence/1

BBa_B0030
type: ComponentDefinition
persistent identity: BBa B0030
display id: BBa_B0030
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS.1 (strong) -- modified from R. Weiss
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _44_46_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("orig" in figure 4-14 of Ron Weiss thesis). No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info Brian Chow
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: BBa_B0030
sequence annotation: RBS
sequence annotation: RBS-1\Strong
sequence: https://synbiohub.org/public/igem/BBa_B0030_sequence/1

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation7025
display id: annotation7025
version: 1
top level: BBa_B0030
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_B0030/annotation7025/range7025/1

https://synbiohub.org/public/igem/BBa_B0030/annotation7025/range7025/1
type: Range
persistent identity: range7025
display id: range7025
version: 1
direction: direction/forward
top level: BBa_B0030
start: 1
end: 15
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1702
display id: annotation1702
version: 1
top level: BBa_B0030
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_B0030/annotation1702/range1702/1

https://synbiohub.org/public/igem/BBa_B0030/annotation1702/range1702/1
type: Range
persistent identity: range1702
display id: range1702
version: 1
direction: direction/forward
top level: BBa_B0030
start: 8
end: 12
orientation: inline

RBS-1\Strong
type: SequenceAnnotation
persistent identity: annotation1701
display id: annotation1701
version: 1
top level: BBa_B0030
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_B0030/annotation1701/range1701/1

https://synbiohub.org/public/igem/BBa_B0030/annotation1701/range1701/1
type: Range
persistent identity: range1701
display id: range1701
version: 1
direction: direction/forward
top level: BBa_B0030
start: 1
end: 15
orientation: inline

Pctc
type: ComponentDefinition
persistent identity: BBa K143010
display id: BBa_K143010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter ctc for B. subtilis
creator: James Chappell
created: 2008-09-10T11:00:00Z
modified: 2015-05-08T01:10:23Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _199_
m user id: 0
owner id: 2090
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Promoter ctc is a sigma factor B dependent promoter found in ''B.subtilis''. In ''B.subtilis'' endogenous sigma factor B is activated under mild stress. These mild stress conditions can be generally split into nutrient stress response and physical stress response. Nutrient stress response is triggered by low levels of ATP and GTP and physical stress response is triggered by exposure to blue light, salt, heat, acid or ethanol1. The promoter ctc has been used previously as a read out for the activation of sigma factor B 2. *The context with which we used the promoter ctc, was to take blue light as an input and give '''Polymerase Per Second'''(PoPS) as an output. To do this the other potential inputs need to be carefully controlled so that only blue light activated the sigma B and gives a PoPS output. In order to get sufficient sigma B activation by blue light the light receptor YtvA, part...., needs to be over expressed in ''B.subtilis'' 3. ===Reference=== #1 pmid=16267279 #2 pmid=3100810 #3 pmid=17575448
mutable notes: Biobrick standard was applied to the promoter ctc sequence.
mutable provenance: The part was designed using the sequence from the ''B.subtilis'' genome and from previously published papers 23. This sequence was then synthesised by Geneart.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: Sigma B -10
sequence annotation: Sigma B -35
sequence: https://synbiohub.org/public/igem/BBa_K143010_sequence/1

Sigma B -10
type: SequenceAnnotation
persistent identity: annotation1975700
display id: annotation1975700
version: 1
top level: Pctc
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K143010/annotation1975700/range1975700/1

https://synbiohub.org/public/igem/BBa_K143010/annotation1975700/range1975700/1
type: Range
persistent identity: range1975700
display id: range1975700
version: 1
direction: direction/forward
top level: Pctc
start: 41
end: 46
orientation: inline

Sigma B -35
type: SequenceAnnotation
persistent identity: annotation1975701
display id: annotation1975701
version: 1
top level: Pctc
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K143010/annotation1975701/range1975701/1

https://synbiohub.org/public/igem/BBa_K143010/annotation1975701/range1975701/1
type: Range
persistent identity: range1975701
display id: range1975701
version: 1
direction: direction/forward
top level: Pctc
start: 21
end: 28
orientation: inline

BBa_K113011
type: ComponentDefinition
persistent identity: BBa K113011
display id: BBa_K113011
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: more overlapping T7 promoter
creator: Wu Jingjing, Wang Jinyu
created: 2008-10-25T11:00:00Z
modified: 2015-05-08T01:09:20Z
discontinued: false
dominant: false
experience: experience/None
group u list: _254_
m user id: 0
owner id: 2711
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Two T7 promoters are reversely overlapping in five base pairs, with the third one mutated from A to G.
mutable notes: It is difficult to make this short DNA by PCR as both sides are T7 promoters, which are very similar. It is highly possible that they form loops before they can be synthesized by PCR. Thus we order two single stranded DNA and then anneal them together by touchdown PCR.
mutable provenance: The sequence of T7 promoter is TAATACGACTCACTATA (from 5' to 3').
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: T7 promoter
sequence: https://synbiohub.org/public/igem/BBa_K113011_sequence/1

T7 promoter
type: SequenceAnnotation
persistent identity: annotation1989359
display id: annotation1989359
version: 1
top level: BBa_K113011
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K113011/annotation1989359/range1989359/1

https://synbiohub.org/public/igem/BBa_K113011/annotation1989359/range1989359/1
type: Range
persistent identity: range1989359
display id: range1989359
version: 1
direction: direction/reverse
top level: BBa_K113011
start: 4
end: 21
orientation: inline

T7 promoter
type: SequenceAnnotation
persistent identity: annotation1989380
display id: annotation1989380
version: 1
top level: BBa_K113011
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K113011/annotation1989380/range1989380/1

https://synbiohub.org/public/igem/BBa_K113011/annotation1989380/range1989380/1
type: Range
persistent identity: range1989380
display id: range1989380
version: 1
direction: direction/forward
top level: BBa_K113011
start: 17
end: 34
orientation: inline

BBa_J61130
type: ComponentDefinition
persistent identity: BBa J61130
display id: BBa_J61130
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ribosome Binding Site Family Member
creator: John Anderson
created: 2007-04-24T11:00:00Z
modified: 2015-08-31T01:59:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable description: {{JCA_Arkin_RBSFamily}}
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J61130_sequence/1

BBa_K777003
type: ComponentDefinition
persistent identity: BBa K777003
display id: BBa_K777003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Tar receptor under the control of constitutive promoter J23105
creator: Bianca Genenncher
created: 2012-09-20T11:00:00Z
modified: 2015-05-08T01:13:16Z
discontinued: false
dominant: false
group u list: _1029_
m user id: 0
owner id: 11946
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: The transmembrane chemoreceptor Tar of E. coli mediates chemotaxis towards aspartate and exists as a functional homodimer. Here we used a set of 8 constitutive [http://partsregistry.org/Part:BBa_J23105 Anderson promoters] from the 2006 Berkeley group to test how different levels of constitutive ''Tar'' expression affect the chemotaxis of ''E. coli''.
mutable notes: * Genomic sequence was amplified using the following primers. ** Primers PCR: Base pairs in caps represent the respective restriction sites followed by either prefix or suffix. Primers were provided by SIGMA. *** Fwd: Tar for + prefix: 5?? actGAATTCgcggccgctTCTAGAtgattaaccgtatccgcgtagtc 3?? *** Rev: Tar rev + suffix: 5?? tcCTGCAGcggccgctACTAGTcaaaatgtttcccagtttggatc 3?? * One XbaI site was found at position 420. The restriction site was changed from TCTAGt to CCTAGt via QuikChange PCR. ** Primers QuikChange (QC): Primers were provided by SIGMA. *** Fwd: TarQC for: 5?? ACTGATTGATTAcCTAGATTATGGCAATACTGGAG 3?? *** Rev: TarQC rev: 5?? TGCCATAATCTAGgTAATCAATCAGTTCAGTTAAC 3?? (the uncapitalized letter induces the mutation for removal of the XbaI site)
mutable provenance: *The part was amplified from genomic DNA of ''E. coli'' str. K-12 substr. DH10B, complete genome (CP000948.1). *J23105 information was taken from the partsregistry and physical DNA from the 2012 distribution kit.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K777000
component: BBa_J23105
sequence annotation: BBa_J23105
sequence annotation: BBa_K777000
sequence: https://synbiohub.org/public/igem/BBa_K777003_sequence/1

BBa_K777000
type: Component
persistent identity: component2189297
display id: component2189297
version: 1
top level: BBa_K777003
access: public
definition: BBa_K777000

BBa_J23105
type: Component
persistent identity: component2189293
display id: component2189293
version: 1
top level: BBa_K777003
access: public
definition: BBa_J23105

BBa_J23105
type: SequenceAnnotation
persistent identity: annotation2189293
display id: annotation2189293
version: 1
top level: BBa_K777003
location: https://synbiohub.org/public/igem/BBa_K777003/annotation2189293/range2189293/1
component: BBa_J23105

https://synbiohub.org/public/igem/BBa_K777003/annotation2189293/range2189293/1
type: Range
persistent identity: range2189293
display id: range2189293
version: 1
direction: direction/reverse
top level: BBa_K777003
start: 1
end: 35
orientation: inline

BBa_K777000
type: SequenceAnnotation
persistent identity: annotation2189297
display id: annotation2189297
version: 1
top level: BBa_K777003
location: https://synbiohub.org/public/igem/BBa_K777003/annotation2189297/range2189297/1
component: BBa_K777000

https://synbiohub.org/public/igem/BBa_K777003/annotation2189297/range2189297/1
type: Range
persistent identity: range2189297
display id: range2189297
version: 1
direction: direction/reverse
top level: BBa_K777003
start: 42
end: 1703
orientation: inline

BBa_B0073
type: ComponentDefinition
persistent identity: BBa B0073
display id: BBa_B0073
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Specialized RBS
creator: Bartholomew Canton
created: 2006-05-01T11:00:00Z
modified: 2015-08-31T04:07:21Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_6_
m user id: 0
owner id: 135
owning group id: 6
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_B0073_sequence/1

BBa_J61136
type: ComponentDefinition
persistent identity: BBa J61136
display id: BBa_J61136
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ribosome Binding Site Family Member
creator: John Anderson
created: 2007-04-24T11:00:00Z
modified: 2015-08-31T01:59:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable description: {{JCA_Arkin_RBSFamily}}
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J61136_sequence/1

BBa_Z0251
type: ComponentDefinition
persistent identity: BBa Z0251
display id: BBa_Z0251
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: T7 strong promoter
creator: T7.2
created: 2005-04-04T11:00:00Z
modified: 2015-05-08T01:14:55Z
discontinued: false
dominant: false
experience: experience/None
group u list: _10_
m user id: 0
owner id: 250
owning group id: 10
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Promoter for T7 RNA polymerase
mutable provenance: bacteriophage T7
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: consensus sequence
sequence: https://synbiohub.org/public/igem/BBa_Z0251_sequence/1

consensus sequence
type: SequenceAnnotation
persistent identity: annotation1476333
display id: annotation1476333
version: 1
top level: BBa_Z0251
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_Z0251/annotation1476333/range1476333/1

https://synbiohub.org/public/igem/BBa_Z0251/annotation1476333/range1476333/1
type: Range
persistent identity: range1476333
display id: range1476333
version: 1
direction: direction/forward
top level: BBa_Z0251
start: 6
end: 28
orientation: inline

BBa_R0085
type: ComponentDefinition
persistent identity: BBa R0085
display id: BBa_R0085
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: T7 Consensus Promoter Sequence
creator: Barry Canton
created: 2005-02-21T12:00:00Z
modified: 2015-05-08T01:14:15Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_6_
m user id: 0
owner id: 135
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: The T7 promoter should only produce PoPS when the T7 polymerase is also being expressed.
mutable provenance: Sequence obtained from Sri Kosuri
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: Transcription Start Site
sequence annotation: Initiation Region
sequence annotation: Polymerase Binding Region
sequence: https://synbiohub.org/public/igem/BBa_R0085_sequence/1

Transcription Start Site
type: SequenceAnnotation
persistent identity: annotation1721520
display id: annotation1721520
version: 1
top level: BBa_R0085
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_R0085/annotation1721520/range1721520/1

https://synbiohub.org/public/igem/BBa_R0085/annotation1721520/range1721520/1
type: Range
persistent identity: range1721520
display id: range1721520
version: 1
direction: direction/forward
top level: BBa_R0085
start: 18
end: 18
orientation: inline

Initiation Region
type: SequenceAnnotation
persistent identity: annotation1721522
display id: annotation1721522
version: 1
top level: BBa_R0085
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_R0085/annotation1721522/range1721522/1

https://synbiohub.org/public/igem/BBa_R0085/annotation1721522/range1721522/1
type: Range
persistent identity: range1721522
display id: range1721522
version: 1
direction: direction/forward
top level: BBa_R0085
start: 12
end: 23
orientation: inline

Polymerase Binding Region
type: SequenceAnnotation
persistent identity: annotation1721521
display id: annotation1721521
version: 1
top level: BBa_R0085
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0085/annotation1721521/range1721521/1

https://synbiohub.org/public/igem/BBa_R0085/annotation1721521/range1721521/1
type: Range
persistent identity: range1721521
display id: range1721521
version: 1
direction: direction/forward
top level: BBa_R0085
start: 1
end: 11
orientation: inline

BBa_K777106
type: ComponentDefinition
persistent identity: BBa K777106
display id: BBa_K777106
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: flhDC operon under the control of constitutive promoter J23112
creator: Team Goettingen
created: 2012-09-18T11:00:00Z
modified: 2015-05-08T01:13:16Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1029_
m user id: 0
owner id: 12106
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: The ''flhDC'' operon is the master regulator of motility and chemotaxis in ''E. coli''. FlhDC forms heterotetramers and activates class II operons in concert with sigma factor 70. Among the gene products of class II operons are several components of the flagellum and the alternative sigma factor FliA which is essential for the transcription of class III genes. Here we used a set of 8 constitutive Anderson-promoters from the 2006 Berkeley group to test how different levels of constitutive ''flhDC'' expression affect the motility of ''E. coli''.
mutable notes: *The PstI restriction site in the ''flhDC'' sequence was mutated to CTGCGG using the reverse primer. *''flhDC'' was amplified from genomic DNA of ''E. coli'' DH10B via PCR using the following primers: **Fwd: 5'-actgaattcgcggccgcttctagatgcatacctccgagttgctg-3' **Rev: 5'-tcctgcagcggccgctactagttactgcccgggatggcggttgacataagcCgcaggcaaagctgccaacag-3' (the capitalized "C" induces the mutation for removal of the PstI site)
mutable provenance: *The part was amplified from genomic DNA of ''E. coli'' str. K-12 substr. DH10B, complete genome (CP000948.1). *J23112 information was taken from the partsregistry and physical DNA from the 2012 distribution kit.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K777100
component: BBa_J23112
sequence annotation: BBa_K777100
sequence annotation: BBa_J23112
sequence: https://synbiohub.org/public/igem/BBa_K777106_sequence/1

BBa_K777100
type: Component
persistent identity: component2187094
display id: component2187094
version: 1
top level: BBa_K777106
access: public
definition: https://synbiohub.org/public/igem/BBa_K777100/1

BBa_J23112
type: Component
persistent identity: component2187086
display id: component2187086
version: 1
top level: BBa_K777106
access: public
definition: BBa_J23112

BBa_K777100
type: SequenceAnnotation
persistent identity: annotation2187094
display id: annotation2187094
version: 1
top level: BBa_K777106
location: https://synbiohub.org/public/igem/BBa_K777106/annotation2187094/range2187094/1
component: BBa_K777100

https://synbiohub.org/public/igem/BBa_K777106/annotation2187094/range2187094/1
type: Range
persistent identity: range2187094
display id: range2187094
version: 1
direction: direction/reverse
top level: BBa_K777106
start: 42
end: 910
orientation: inline

BBa_J23112
type: SequenceAnnotation
persistent identity: annotation2187086
display id: annotation2187086
version: 1
top level: BBa_K777106
location: https://synbiohub.org/public/igem/BBa_K777106/annotation2187086/range2187086/1
component: BBa_J23112

https://synbiohub.org/public/igem/BBa_K777106/annotation2187086/range2187086/1
type: Range
persistent identity: range2187086
display id: range2187086
version: 1
direction: direction/reverse
top level: BBa_K777106
start: 1
end: 35
orientation: inline

BBa_K319005
type: ComponentDefinition
persistent identity: BBa K319005
display id: BBa_K319005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: yeast mid-length ADH1 promoter
creator: Afnan Azizi
created: 2010-10-15T11:00:00Z
modified: 2015-05-08T01:11:58Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _437_
m user id: 0
owner id: 7363
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This is the full length ADH1 promoter including the distal and proximal portions and their regulatory elements.
mutable notes: There were three options for the length of the promoter - short, middle, and long. The middle length was chosen.
mutable provenance: The part was extracted from S. cerevisiae, YPH500 strain, using colony PCR protocols. It consists of 720 bp upstream of ADH1 gene according to SGD sequence.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: ADH1 promoter
sequence: https://synbiohub.org/public/igem/BBa_K319005_sequence/1

ADH1 promoter
type: SequenceAnnotation
persistent identity: annotation2112269
display id: annotation2112269
version: 1
top level: BBa_K319005
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K319005/annotation2112269/range2112269/1

https://synbiohub.org/public/igem/BBa_K319005/annotation2112269/range2112269/1
type: Range
persistent identity: range2112269
display id: range2112269
version: 1
direction: direction/forward
top level: BBa_K319005
start: 1
end: 720
orientation: inline

BBa_K777102
type: ComponentDefinition
persistent identity: BBa K777102
display id: BBa_K777102
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: flhDC operon under the control of constitutive promoter J23104
creator: Team Goettingen
created: 2012-09-18T11:00:00Z
modified: 2015-05-08T01:13:16Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1029_
m user id: 0
owner id: 12106
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: The flhDC operon is the master regulator of motility and chemotaxis in E. coli. FlhDC forms heterotetramers and activates class II operons in concert with sigma factor 70. Among the gene products of class II operons are several components of the flagellum and the alternative sigma factor FliA which is essential for the transcription of class III genes. Here we used a set of 8 constitutive Anderson-promoters from the 2006 Berkeley group to test how different levels of constitutive flhDC expression affect the motility of E. coli.
mutable notes: * The PstI restriction site in the flhDC sequence was mutated to CTGCGG using the reverse primer * flhDC was amplified from genomic DNA of E. coli DH10B via PCR using the following primers: ** Fwd: 5'-actgaattcgcggccgcttctagatgcatacctccgagttgctg-3' ** Rev: 5'-tcctgcagcggccgctactagttactgcccgggatggcggttgacataagcCgcaggcaaagctgccaacag-3' (the capitalized "C" induces the mutation for removal of the PstI site)
mutable provenance: * The part was amplified from genomic DNA of E. coli str. K-12 substr. DH10B, complete genome (CP000948.1). * J23100 information was taken from the partsregistry and physical DNA from the 2012 distribution kit.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K777100
component: BBa_J23104
sequence annotation: BBa_K777100
sequence annotation: BBa_J23104
sequence: https://synbiohub.org/public/igem/BBa_K777102_sequence/1

BBa_K777100
type: Component
persistent identity: component2187014
display id: component2187014
version: 1
top level: BBa_K777102
access: public
definition: https://synbiohub.org/public/igem/BBa_K777100/1

BBa_J23104
type: Component
persistent identity: component2187006
display id: component2187006
version: 1
top level: BBa_K777102
access: public
definition: BBa_J23104

BBa_K777100
type: SequenceAnnotation
persistent identity: annotation2187014
display id: annotation2187014
version: 1
top level: BBa_K777102
location: https://synbiohub.org/public/igem/BBa_K777102/annotation2187014/range2187014/1
component: BBa_K777100

https://synbiohub.org/public/igem/BBa_K777102/annotation2187014/range2187014/1
type: Range
persistent identity: range2187014
display id: range2187014
version: 1
direction: direction/reverse
top level: BBa_K777102
start: 42
end: 910
orientation: inline

BBa_J23104
type: SequenceAnnotation
persistent identity: annotation2187006
display id: annotation2187006
version: 1
top level: BBa_K777102
location: https://synbiohub.org/public/igem/BBa_K777102/annotation2187006/range2187006/1
component: BBa_J23104

https://synbiohub.org/public/igem/BBa_K777102/annotation2187006/range2187006/1
type: Range
persistent identity: range2187006
display id: range2187006
version: 1
direction: direction/reverse
top level: BBa_K777102
start: 1
end: 35
orientation: inline

BBa_J61133
type: ComponentDefinition
persistent identity: BBa J61133
display id: BBa_J61133
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ribosome Binding Site Family Member
creator: John Anderson
created: 2007-04-24T11:00:00Z
modified: 2015-08-31T01:59:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable description: {{JCA_Arkin_RBSFamily}}
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J61133_sequence/1

BBa_J61108
type: ComponentDefinition
persistent identity: BBa J61108
display id: BBa_J61108
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ribosome Binding Site Family Member
creator: John Anderson
created: 2007-04-23T11:00:00Z
modified: 2015-08-31T02:03:00Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable description: {{JCA_Arkin_RBSFamily}}
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J61108_sequence/1

BBa_J61145
type: ComponentDefinition
persistent identity: BBa J61145
display id: BBa_J61145
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: {rbs1} Library Member in BBb
creator: John Anderson
created: 2007-09-03T11:00:00Z
modified: 2015-08-31T01:56:25Z
discontinued: false
dominant: false
experience: experience/None
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: {{BBbRBS1Family}}
mutable notes: {{BerkiGEM2007-Biobrick2.0Intro}}
mutable provenance: Synthetic
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J61145_sequence/1

BBa_J61118
type: ComponentDefinition
persistent identity: BBa J61118
display id: BBa_J61118
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ribosome Binding Site Family Member
creator: John Anderson
created: 2007-04-23T11:00:00Z
modified: 2015-08-31T01:59:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable description: {{JCA_Arkin_RBSFamily}}
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J61118_sequence/1

BBa_B0041
type: ComponentDefinition
persistent identity: BBa B0041
display id: BBa_B0041
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS 3
creator: Josh Michener
created: 2005-12-20T12:00:00Z
modified: 2015-08-31T04:07:20Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_
m user id: 0
owner id: 253
owning group id: 6
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Modified version of B0031 from the Voigt Lab. Not currently available as an independent part (only in a tet inverter).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_B0041_sequence/1

BBa_M13504
type: ComponentDefinition
persistent identity: BBa M13504
display id: BBa_M13504
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: M13K07 gene IV RBS
creator: Natalie Kuldell
created: 2006-12-14T12:00:00Z
modified: 2015-05-08T01:13:56Z
discontinued: false
dominant: false
experience: experience/None
group u list: _45_
m user id: 0
owner id: 314
owning group id: 1
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This RBS is part of the bacteriophage M13 genome, initiating translation of the gene IV message (BBa_M13004). The part aligns with base pairs 4204-4219 in M13K07 genome. It was identified as the RBS for gene IV message by Wezenbeek et al in Gene (1980) 11: 129-148
mutable notes: none
mutable provenance: New England Biolabs M13K07. Sequenced in 2002 by L. Panganaban
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_M13504_sequence/1

BBa_K823005
type: ComponentDefinition
persistent identity: BBa K823005
display id: BBa_K823005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Anderson promoter J23101
creator: Korinna Kraft
created: 2012-09-07T11:00:00Z
modified: 2015-07-07T11:44:25Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1081_
m user id: 4206
owner id: 12081
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Anderson promoter J23101 without RFP in backbone vector pSB1C3 to easily fuse the promoter with other reporters e.g. the lux operon.
mutable notes: no considerations
mutable provenance: Partsregistry
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: J23101
sequence annotation: -35 box
sequence annotation: -10 box
sequence: https://synbiohub.org/public/igem/BBa_K823005_sequence/1

J23101
type: SequenceAnnotation
persistent identity: annotation2182509
display id: annotation2182509
version: 1
top level: BBa_K823005
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K823005/annotation2182509/range2182509/1

https://synbiohub.org/public/igem/BBa_K823005/annotation2182509/range2182509/1
type: Range
persistent identity: range2182509
display id: range2182509
version: 1
direction: direction/forward
top level: BBa_K823005
start: 1
end: 35
orientation: inline

-35 box
type: SequenceAnnotation
persistent identity: annotation2182510
display id: annotation2182510
version: 1
top level: BBa_K823005
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K823005/annotation2182510/range2182510/1

https://synbiohub.org/public/igem/BBa_K823005/annotation2182510/range2182510/1
type: Range
persistent identity: range2182510
display id: range2182510
version: 1
direction: direction/forward
top level: BBa_K823005
start: 1
end: 6
orientation: inline

-10 box
type: SequenceAnnotation
persistent identity: annotation2182511
display id: annotation2182511
version: 1
top level: BBa_K823005
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K823005/annotation2182511/range2182511/1

https://synbiohub.org/public/igem/BBa_K823005/annotation2182511/range2182511/1
type: Range
persistent identity: range2182511
display id: range2182511
version: 1
direction: direction/forward
top level: BBa_K823005
start: 24
end: 29
orientation: inline

BBa_J61101
type: ComponentDefinition
persistent identity: BBa J61101
display id: BBa_J61101
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ribosome Binding Site Family Member
creator: John Anderson
created: 2007-01-28T12:00:00Z
modified: 2015-08-31T02:03:00Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable description: fix
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J61101_sequence/1

BBa_I723014
type: ComponentDefinition
persistent identity: BBa I723014
display id: BBa_I723014
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Estimated RBS for DntA
creator: Scott Ramsay
created: 2007-10-24T11:00:00Z
modified: 2015-08-31T04:07:53Z
discontinued: false
dominant: false
experience: experience/None
group u list: _126_
m user id: 0
owner id: 2140
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is the naturally-occurring RBS found in the regulatory region of the DntA gene.
mutable notes: '''''This does not physically exist as a basic part due to time constraints; it was cloned as part of composite part which incorporates all of the components necessary for expression of a reporter gene under the control of DntR'''''. For this reason, the ends of the supplied sequence may not accurately represent the boundaries of the functional unit.
mutable provenance: Cloned from a historical plasmid based on pQF52 carrying genomic sequences from ''Burkholderia cepacia''.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: misc
sequence: https://synbiohub.org/public/igem/BBa_I723014_sequence/1

misc
type: SequenceAnnotation
persistent identity: annotation1954961
display id: annotation1954961
version: 1
top level: BBa_I723014
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I723014/annotation1954961/range1954961/1

https://synbiohub.org/public/igem/BBa_I723014/annotation1954961/range1954961/1
type: Range
persistent identity: range1954961
display id: range1954961
version: 1
direction: direction/forward
top level: BBa_I723014
start: 1
end: 25
orientation: inline

BBa_K1484214
type: ComponentDefinition
persistent identity: BBa K1484214
display id: BBa_K1484214
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: MpEF1a (Marchantia poylmorpha Elongation Factor 1, PlantSyntax Pro+5U)
creator: Salil Bhate
created: 2014-10-07T11:00:00Z
modified: 2015-06-10T02:07:44Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1864_
m user id: 4206
owner id: 21057
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Mp Elongation Factor 1 Promoter + 5U
mutable notes: 5UTR
mutable provenance: MP Genome
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: PlantSyntax Pro+5U
sequence: https://synbiohub.org/public/igem/BBa_K1484214_sequence/1

PlantSyntax Pro+5U
type: SequenceAnnotation
persistent identity: annotation2414232
display id: annotation2414232
version: 1
top level: BBa_K1484214
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1484214/annotation2414232/range2414232/1

https://synbiohub.org/public/igem/BBa_K1484214/annotation2414232/range2414232/1
type: Range
persistent identity: range2414232
display id: range2414232
version: 1
direction: direction/forward
top level: BBa_K1484214
start: 1745
end: 1756
orientation: inline

PlantSyntax Pro+5U
type: SequenceAnnotation
persistent identity: annotation2414230
display id: annotation2414230
version: 1
top level: BBa_K1484214
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1484214/annotation2414230/range2414230/1

https://synbiohub.org/public/igem/BBa_K1484214/annotation2414230/range2414230/1
type: Range
persistent identity: range2414230
display id: range2414230
version: 1
direction: direction/forward
top level: BBa_K1484214
start: 1
end: 11
orientation: inline

BBa_J23107
type: ComponentDefinition
persistent identity: BBa J23107
display id: BBa_J23107
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: constitutive promoter family member
creator: John Anderson
created: 2006-08-16T11:00:00Z
modified: 2015-08-31T04:08:40Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: Later
mutable notes: N/A
mutable provenance: Later
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J23107_sequence/1

CP1LacPI1
type: ComponentDefinition
persistent identity: BBa K418000
display id: BBa_K418000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: IPTG inducible Lac promoter cassette
creator: Northwestern University iGEM 2010
created: 2010-10-11T11:00:00Z
modified: 2015-05-08T01:12:28Z
discontinued: false
dominant: true
experience: experience/None
group u list: _532_
m user id: 0
owner id: 6657
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: constituitive promoter + LacPI
mutable notes: blah
mutable provenance: From assembly of kit parts.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_Q01121
component: BBa_J23100
sequence annotation: BBa_J23100
sequence annotation: BBa_Q01121
sequence: https://synbiohub.org/public/igem/BBa_K418000_sequence/1

BBa_Q01121
type: Component
persistent identity: component2263742
display id: component2263742
version: 1
top level: CP1LacPI1
access: public
definition: https://synbiohub.org/public/igem/BBa_Q01121/1

BBa_J23100
type: Component
persistent identity: component2263723
display id: component2263723
version: 1
top level: CP1LacPI1
access: public
definition: BBa_J23100

BBa_J23100
type: SequenceAnnotation
persistent identity: annotation2263723
display id: annotation2263723
version: 1
top level: CP1LacPI1
location: https://synbiohub.org/public/igem/BBa_K418000/annotation2263723/range2263723/1
component: BBa_J23100

https://synbiohub.org/public/igem/BBa_K418000/annotation2263723/range2263723/1
type: Range
persistent identity: range2263723
display id: range2263723
version: 1
direction: direction/reverse
top level: CP1LacPI1
start: 1
end: 35
orientation: inline

BBa_Q01121
type: SequenceAnnotation
persistent identity: annotation2263742
display id: annotation2263742
version: 1
top level: CP1LacPI1
location: https://synbiohub.org/public/igem/BBa_K418000/annotation2263742/range2263742/1
component: BBa_Q01121

https://synbiohub.org/public/igem/BBa_K418000/annotation2263742/range2263742/1
type: Range
persistent identity: range2263742
display id: range2263742
version: 1
direction: direction/reverse
top level: CP1LacPI1
start: 44
end: 1416
orientation: inline

BBa_K1585100
type: ComponentDefinition
persistent identity: BBa K1585100
display id: BBa_K1585100
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Anderson Promoter with lacI binding site
creator: Tobias Schwanemann
created: 2015-08-23T11:00:00Z
modified: 2015-08-26T11:28:17Z
discontinued: false
dominant: false
experience: experience/Fails
group u list: _2002_
m user id: 24474
owner id: 24474
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Promoters of the Anderson Promoter Collection with lacI binding site derived from [http://parts.igem.org/Part:BBa_R0010 R_0010] to make them [http://openwetware.org/wiki/IPTG IPTG] iducible.
mutable notes: The "lacI binding site" of Part [http://parts.igem.org/Part:BBa_R0010 R_0010] differs from lacO from Part [http://parts.igem.org/Part:BBa_J33207 BBa_J33207] although they should be same. We choosed part BBa_R0010 because we assumed older parts would be reliable.
mutable provenance: Assambly of [http://parts.igem.org/Part:BBa_J23100 J23100] and lacI-binding-site of [http://parts.igem.org/Part:BBa_R0010 R_0010]
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
component: BBa_K1585999
component: BBa_J23100
sequence annotation: BBa_K1585999
sequence annotation: BBa_J23100
sequence: https://synbiohub.org/public/igem/BBa_K1585100_sequence/1

BBa_K1585999
type: Component
persistent identity: component2436913
display id: component2436913
version: 1
top level: BBa_K1585100
access: public
definition: https://synbiohub.org/public/igem/BBa_K1585999/1

BBa_J23100
type: Component
persistent identity: component2436912
display id: component2436912
version: 1
top level: BBa_K1585100
access: public
definition: BBa_J23100

BBa_K1585999
type: SequenceAnnotation
persistent identity: annotation2436913
display id: annotation2436913
version: 1
top level: BBa_K1585100
location: https://synbiohub.org/public/igem/BBa_K1585100/annotation2436913/range2436913/1
component: BBa_K1585999

https://synbiohub.org/public/igem/BBa_K1585100/annotation2436913/range2436913/1
type: Range
persistent identity: range2436913
display id: range2436913
version: 1
direction: direction/reverse
top level: BBa_K1585100
start: 44
end: 78
orientation: inline

BBa_J23100
type: SequenceAnnotation
persistent identity: annotation2436912
display id: annotation2436912
version: 1
top level: BBa_K1585100
location: https://synbiohub.org/public/igem/BBa_K1585100/annotation2436912/range2436912/1
component: BBa_J23100

https://synbiohub.org/public/igem/BBa_K1585100/annotation2436912/range2436912/1
type: Range
persistent identity: range2436912
display id: range2436912
version: 1
direction: direction/reverse
top level: BBa_K1585100
start: 1
end: 35
orientation: inline

BBa_B0036
type: ComponentDefinition
persistent identity: BBa B0036
display id: BBa_B0036
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Specialized RBS
creator: Barry Canton
created: 2004-06-07T11:00:00Z
modified: 2015-08-31T04:07:20Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_6_
m user id: 0
owner id: 135
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This RBS is recognized by ribosomes carrying the modified anti-Shine Dalgarno sequence as found in Brink et al. (see below)
mutable provenance: Brink MF, Verbeet MP, de Boer HA (1995). Specialized ribosomes: highly specific translation in vivo of a single targetted mRNA species. Gene, 156, pp. 215-222
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_B0036_sequence/1

BBa_M31201
type: ComponentDefinition
persistent identity: BBa M31201
display id: BBa_M31201
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Yeast CLB1 promoter region, G2/M cell cycle specific
creator: Katherine Aull
created: 2008-05-13T11:00:00Z
modified: 2015-05-08T01:13:59Z
discontinued: false
dominant: false
group u list: _89_
m user id: 0
owner id: 624
owning group id: 89
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Description of CLB1 (from SGD): B-type cyclin involved in cell cycle progression; activates Cdc28p to promote the transition from G2 to M phase; accumulates during G2 and M, then targeted via a destruction box motif for ubiquitin-mediated degradation by the proteasome. More information found here: http://db.yeastgenome.org/cgi-bin/locus.pl?locus=CLB1
mutable notes: Part contains 500 bases upstream from the CLB1 ORF. Is NOT a minimal promoter, and has not been tested for composability.
mutable provenance: Yeast consensus sequence from SGD chrVII:703140..703639
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_M31201_sequence/1

BBa_J61137
type: ComponentDefinition
persistent identity: BBa J61137
display id: BBa_J61137
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ribosome Binding Site Family Member
creator: John Anderson
created: 2007-04-24T11:00:00Z
modified: 2015-08-31T01:59:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable description: {{JCA_Arkin_RBSFamily}}
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J61137_sequence/1

RBS-spoVG
type: ComponentDefinition
persistent identity: BBa K143021
display id: BBa_K143021
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: SpoVG ribosome binding site (RBS) for B. subtilis
creator: James Chappell
created: 2008-09-16T11:00:00Z
modified: 2015-05-08T01:10:23Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _199_
m user id: 0
owner id: 2090
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Description: SpoVG is an endogenous ribosome binding site from B.subtilis. The sequence of the spoVG ribosome binding site is AAAGGUGGUGA which is complementary to the sequence UUUCCUCCACU from the 3' region of the 16s rRNA from B.subtilis. Previous research showed that the predicted binding energy of the 16s rRNA to the RBS is -19kcal 1
mutable notes: In order to ensure that the RBS is functional the actual ribosome binding site was maintained and the distance between the RBS and the start codon maintained. In order to conform to the biobrick standard the sequence flanking the RBS had to be changed but the distance between the promoter and RBS, and start codon and RBS was maintained.
mutable provenance: The sequence was taken from a previous research paper [1] and was constructed by Geneart.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: rbs
sequence: https://synbiohub.org/public/igem/BBa_K143021_sequence/1

rbs
type: SequenceAnnotation
persistent identity: annotation1975997
display id: annotation1975997
version: 1
top level: RBS-spoVG
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K143021/annotation1975997/range1975997/1

https://synbiohub.org/public/igem/BBa_K143021/annotation1975997/range1975997/1
type: Range
persistent identity: range1975997
display id: range1975997
version: 1
direction: direction/forward
top level: RBS-spoVG
start: 1
end: 12
orientation: inline

BBa_J64026
type: ComponentDefinition
persistent identity: BBa J64026
display id: BBa_J64026
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: sigE RBS
creator: Dan Widmaier
created: 2008-01-16T12:00:00Z
modified: 2015-08-31T01:56:26Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _98_
m user id: 0
owner id: 2407
owning group id: 98
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is the natural RBS for the sigE chaperone (BBa_J64025) in Salmonella Typhimurium LT2.
mutable notes: This part was not designed with compatibility to biobrick standard assembly in mind. This RBS has not been explicitly assayed for expression rate.
mutable provenance: Salmonella LT2 Genomic DNA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: sigE RBS
sequence: https://synbiohub.org/public/igem/BBa_J64026_sequence/1

sigE RBS
type: SequenceAnnotation
persistent identity: annotation1959625
display id: annotation1959625
version: 1
top level: BBa_J64026
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_J64026/annotation1959625/range1959625/1

https://synbiohub.org/public/igem/BBa_J64026/annotation1959625/range1959625/1
type: Range
persistent identity: range1959625
display id: range1959625
version: 1
direction: direction/forward
top level: BBa_J64026
start: 1
end: 18
orientation: inline

BBa_M13106
type: ComponentDefinition
persistent identity: BBa M13106
display id: BBa_M13106
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: M13K07 gene VI promoter
creator: Natalie Kuldell
created: 2006-12-15T12:00:00Z
modified: 2015-05-08T01:13:55Z
discontinued: false
dominant: false
group u list: _45_
m user id: 0
owner id: 314
owning group id: 1
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This part is from the bacteriophage M13 genome, bp 2716-2764 in M13K07. It directs transcription of M13 gene VI (BBa_M13506, BBa_M13006). Its identity as a promoter was described in Gene (1980) 11:129-148 based on -10,-35 homology and fragment of genome able to bind RNAP.
mutable notes: none
mutable provenance: New England Biolabs M13K07. Sequenced in 2002 by L. Panganaban.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_M13106_sequence/1

BBa_K777107
type: ComponentDefinition
persistent identity: BBa K777107
display id: BBa_K777107
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: flhDC operon under the control of constitutive promoter J23113
creator: Team Goettingen
created: 2012-09-18T11:00:00Z
modified: 2015-05-08T01:13:16Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1029_
m user id: 0
owner id: 12106
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: The ''flhDC'' operon is the master regulator of motility and chemotaxis in ''E. coli''. FlhDC forms heterotetramers and activates class II operons in concert with sigma factor 70. Among the gene products of class II operons are several components of the flagellum and the alternative sigma factor FliA which is essential for the transcription of class III genes. Here we used a set of 8 constitutive Anderson-promoters from the 2006 Berkeley group to test how different levels of constitutive ''flhDC'' expression affect the motility of ''E. coli''.
mutable notes: *The PstI restriction site in the ''flhDC'' sequence was mutated to CTGCGG using the reverse primer. *''flhDC'' was amplified from genomic DNA of ''E. coli'' DH10B via PCR using the following primers: **Fwd: 5'-actgaattcgcggccgcttctagatgcatacctccgagttgctg-3' **Rev: 5'-tcctgcagcggccgctactagttactgcccgggatggcggttgacataagcCgcaggcaaagctgccaacag-3' (the capitalized "C" induces the mutation for removal of the PstI site)
mutable provenance: *The part was amplified from genomic DNA of ''E. coli'' str. K-12 substr. DH10B, complete genome (CP000948.1). *J23113 information was taken from the partsregistry and physical DNA from the 2012 distribution kit.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_J23113
component: BBa_K777100
sequence annotation: BBa_K777100
sequence annotation: BBa_J23113
sequence: https://synbiohub.org/public/igem/BBa_K777107_sequence/1

BBa_J23113
type: Component
persistent identity: component2187095
display id: component2187095
version: 1
top level: BBa_K777107
access: public
definition: BBa_J23113

BBa_K777100
type: Component
persistent identity: component2187103
display id: component2187103
version: 1
top level: BBa_K777107
access: public
definition: https://synbiohub.org/public/igem/BBa_K777100/1

BBa_K777100
type: SequenceAnnotation
persistent identity: annotation2187103
display id: annotation2187103
version: 1
top level: BBa_K777107
location: https://synbiohub.org/public/igem/BBa_K777107/annotation2187103/range2187103/1
component: BBa_K777100

https://synbiohub.org/public/igem/BBa_K777107/annotation2187103/range2187103/1
type: Range
persistent identity: range2187103
display id: range2187103
version: 1
direction: direction/reverse
top level: BBa_K777107
start: 42
end: 910
orientation: inline

BBa_J23113
type: SequenceAnnotation
persistent identity: annotation2187095
display id: annotation2187095
version: 1
top level: BBa_K777107
location: https://synbiohub.org/public/igem/BBa_K777107/annotation2187095/range2187095/1
component: BBa_J23113

https://synbiohub.org/public/igem/BBa_K777107/annotation2187095/range2187095/1
type: Range
persistent identity: range2187095
display id: range2187095
version: 1
direction: direction/reverse
top level: BBa_K777107
start: 1
end: 35
orientation: inline

Pmra
type: ComponentDefinition
persistent identity: BBa S03331
display id: BBa_S03331
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: --Specify Parts List--
creator: Simone Macmil
created: 2005-09-01T11:00:00Z
modified: 2015-05-08T01:14:21Z
discontinued: false
dominant: false
group u list: _39_
m user id: 0
owner id: 318
owning group id: 39
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Promoter for cell division genes ftsI and ftsW
mutable notes: BBa_S03331is based on the reguatory region preceding cell division genes in the mra cluster and includes the biobrick head and tail restriction sites.
mutable provenance: A promoter for the first nine genes of the Escherichia coli mra cluster of cell division and cell envelope biosynthesis genes, including ftsI and ftsW. Hara H, Yasuda S, Horiuchi K and Park JT. Journal of Bacteriology (1997) 179: 5802-5811
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_S03331_sequence/1

BBa_J61146
type: ComponentDefinition
persistent identity: BBa J61146
display id: BBa_J61146
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: {rbs1} Library Member in BBb
creator: John Anderson
created: 2007-09-03T11:00:00Z
modified: 2015-08-31T01:56:25Z
discontinued: false
dominant: false
experience: experience/None
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: {{BBbRBS1Family}}
mutable notes: {{BerkiGEM2007-Biobrick2.0Intro}}
mutable provenance: Synthetic
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J61146_sequence/1

BBa_K823004
type: ComponentDefinition
persistent identity: BBa K823004
display id: BBa_K823004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Anderson promoter J23100
creator: Korinna Kraft
created: 2012-09-07T11:00:00Z
modified: 2015-07-07T11:43:33Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1081_
m user id: 4206
owner id: 12081
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Anderson promoter J23100 without RFP in backbone vector pSB1C3 to easily fuse the promoter with other reporters e.g. the lux operon.
mutable notes: no considerations
mutable provenance: Partsregistry
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -35 box
sequence annotation: J23100
sequence annotation: -10 box
sequence: https://synbiohub.org/public/igem/BBa_K823004_sequence/1

-35 box
type: SequenceAnnotation
persistent identity: annotation2182507
display id: annotation2182507
version: 1
top level: BBa_K823004
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K823004/annotation2182507/range2182507/1

https://synbiohub.org/public/igem/BBa_K823004/annotation2182507/range2182507/1
type: Range
persistent identity: range2182507
display id: range2182507
version: 1
direction: direction/forward
top level: BBa_K823004
start: 1
end: 6
orientation: inline

J23100
type: SequenceAnnotation
persistent identity: annotation2182506
display id: annotation2182506
version: 1
top level: BBa_K823004
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K823004/annotation2182506/range2182506/1

https://synbiohub.org/public/igem/BBa_K823004/annotation2182506/range2182506/1
type: Range
persistent identity: range2182506
display id: range2182506
version: 1
direction: direction/forward
top level: BBa_K823004
start: 1
end: 35
orientation: inline

-10 box
type: SequenceAnnotation
persistent identity: annotation2182508
display id: annotation2182508
version: 1
top level: BBa_K823004
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K823004/annotation2182508/range2182508/1

https://synbiohub.org/public/igem/BBa_K823004/annotation2182508/range2182508/1
type: Range
persistent identity: range2182508
display id: range2182508
version: 1
direction: direction/forward
top level: BBa_K823004
start: 24
end: 29
orientation: inline

R38
type: ComponentDefinition
persistent identity: BBa K780001
display id: BBa_K780001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Strong RBS for Bacillus Subtilis
creator: Krzysztof Szczepaniak
created: 2012-09-25T11:00:00Z
modified: 2015-05-08T01:13:18Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1034_
m user id: 0
owner id: 10271
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Natural RBS sequence for Bacillus Subtilis, described in the literature as a strong RBS for this bacteria.
mutable notes: -
mutable provenance: From genomic sequence Bacillus Subtilis.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_K780001_sequence/1

BBa_I712074
type: ComponentDefinition
persistent identity: BBa I712074
display id: BBa_I712074
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: T7 promoter (strong promoter from T7 bacteriophage)
creator: Rok Gaber
created: 2007-10-21T11:00:00Z
modified: 2015-08-31T04:07:46Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _130_
m user id: 0
owner id: 1856
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: T7 promoter is very specific promoter which is transcribed only by specific T7 RNA polymerase. Usually this promoter is used in expression systems where T7 promoter is cotransfected with T7 RNA polymerase. That ensures strong transcription of desired genes.
mutable provenance: T7 bacteriophage
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I712074_sequence/1

BBa_Z0252
type: ComponentDefinition
persistent identity: BBa Z0252
display id: BBa_Z0252
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: T7 weak binding and processivity
creator: T7.2
created: 2005-04-04T11:00:00Z
modified: 2015-05-08T01:14:55Z
discontinued: false
dominant: false
experience: experience/None
group u list: _10_
m user id: 0
owner id: 250
owning group id: 10
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Promoter for T7 RNA polymerase
mutable provenance: T7
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: t -> a mutation from consensus
sequence annotation: tga -> acg mutation from consensus
sequence annotation: 23bp conserved region
sequence: https://synbiohub.org/public/igem/BBa_Z0252_sequence/1

t -> a mutation from consensus
type: SequenceAnnotation
persistent identity: annotation1476345
display id: annotation1476345
version: 1
top level: BBa_Z0252
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_Z0252/annotation1476345/range1476345/1

https://synbiohub.org/public/igem/BBa_Z0252/annotation1476345/range1476345/1
type: Range
persistent identity: range1476345
display id: range1476345
version: 1
direction: direction/forward
top level: BBa_Z0252
start: 21
end: 21
orientation: inline

tga -> acg mutation from consensus
type: SequenceAnnotation
persistent identity: annotation1476344
display id: annotation1476344
version: 1
top level: BBa_Z0252
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_Z0252/annotation1476344/range1476344/1

https://synbiohub.org/public/igem/BBa_Z0252/annotation1476344/range1476344/1
type: Range
persistent identity: range1476344
display id: range1476344
version: 1
direction: direction/forward
top level: BBa_Z0252
start: 10
end: 12
orientation: inline

23bp conserved region
type: SequenceAnnotation
persistent identity: annotation1476343
display id: annotation1476343
version: 1
top level: BBa_Z0252
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_Z0252/annotation1476343/range1476343/1

https://synbiohub.org/public/igem/BBa_Z0252/annotation1476343/range1476343/1
type: Range
persistent identity: range1476343
display id: range1476343
version: 1
direction: direction/forward
top level: BBa_Z0252
start: 6
end: 28
orientation: inline

BBa_K090505
type: ComponentDefinition
persistent identity: BBa K090505
display id: BBa_K090505
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: ''Bacillus subtilis'' consensus RBS
creator: Daniel Goodman
created: 2008-10-27T12:00:00Z
modified: 2015-05-08T01:08:37Z
discontinued: false
dominant: false
experience: experience/None
group u list: _188_
m user id: 0
owner id: 3501
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is the consensus RBS for Bacillus subtilis.
mutable notes: The part must be 8 nucleotides away from the first methionine of the coding sequence, and so we added 'TGT' downstream of the actual binding sequence: AAAGGAGG. This, combined with the truncated non-CDS BioBrick prefix scar, gives exactly 8 'spacer bases' between the 3' end of the RBS and the start of the coding sequence.
mutable provenance: This part was synthesized directly.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: B. subtilis consensus RBS
sequence: https://synbiohub.org/public/igem/BBa_K090505_sequence/1

B. subtilis consensus RBS
type: SequenceAnnotation
persistent identity: annotation1992802
display id: annotation1992802
version: 1
top level: BBa_K090505
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K090505/annotation1992802/range1992802/1

https://synbiohub.org/public/igem/BBa_K090505/annotation1992802/range1992802/1
type: Range
persistent identity: range1992802
display id: range1992802
version: 1
direction: direction/forward
top level: BBa_K090505
start: 1
end: 8
orientation: inline

BBa_J61121
type: ComponentDefinition
persistent identity: BBa J61121
display id: BBa_J61121
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ribosome Binding Site Family Member
creator: John Anderson
created: 2007-04-24T11:00:00Z
modified: 2015-08-31T01:59:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable description: {{JCA_Arkin_RBSFamily}}
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J61121_sequence/1

BBa_J61106
type: ComponentDefinition
persistent identity: BBa J61106
display id: BBa_J61106
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ribosome Binding Site Family Member
creator: John Anderson
created: 2007-04-23T11:00:00Z
modified: 2015-08-31T02:03:00Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable description: {{JCA_Arkin_RBSFamily}}
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J61106_sequence/1

BBa_K777108
type: ComponentDefinition
persistent identity: BBa K777108
display id: BBa_K777108
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: flhDC operon under the control of constitutive promoter J23114
creator: Team Goettingen
created: 2012-09-18T11:00:00Z
modified: 2015-05-08T01:13:16Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1029_
m user id: 0
owner id: 12106
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: The ''flhDC'' operon is the master regulator of motility and chemotaxis in ''E. coli''. FlhDC forms heterotetramers and activates class II operons in concert with sigma factor 70. Among the gene products of class II operons are several components of the flagellum and the alternative sigma factor FliA which is essential for the transcription of class III genes. Here we used a set of 8 constitutive Anderson-promoters from the 2006 Berkeley group to test how different levels of constitutive ''flhDC'' expression affect the motility of ''E. coli''.
mutable notes: *The PstI restriction site in the ''flhDC'' sequence was mutated to CTGCGG using the reverse primer. *''flhDC'' was amplified from genomic DNA of ''E. coli'' DH10B via PCR using the following primers: **Fwd: 5'-actgaattcgcggccgcttctagatgcatacctccgagttgctg-3' **Rev: 5'-tcctgcagcggccgctactagttactgcccgggatggcggttgacataagcCgcaggcaaagctgccaacag-3' (the capitalized "C" induces the mutation for removal of the PstI site)
mutable provenance: *The part was amplified from genomic DNA of ''E. coli'' str. K-12 substr. DH10B, complete genome (CP000948.1). *J23100 information was taken from the partsregistry and physical DNA from the 2012 distribution kit.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_J23114
component: BBa_K777100
sequence annotation: BBa_J23114
sequence annotation: BBa_K777100
sequence: https://synbiohub.org/public/igem/BBa_K777108_sequence/1

BBa_J23114
type: Component
persistent identity: component2187118
display id: component2187118
version: 1
top level: BBa_K777108
access: public
definition: BBa_J23114

BBa_K777100
type: Component
persistent identity: component2187126
display id: component2187126
version: 1
top level: BBa_K777108
access: public
definition: https://synbiohub.org/public/igem/BBa_K777100/1

BBa_J23114
type: SequenceAnnotation
persistent identity: annotation2187118
display id: annotation2187118
version: 1
top level: BBa_K777108
location: https://synbiohub.org/public/igem/BBa_K777108/annotation2187118/range2187118/1
component: BBa_J23114

https://synbiohub.org/public/igem/BBa_K777108/annotation2187118/range2187118/1
type: Range
persistent identity: range2187118
display id: range2187118
version: 1
direction: direction/reverse
top level: BBa_K777108
start: 1
end: 35
orientation: inline

BBa_K777100
type: SequenceAnnotation
persistent identity: annotation2187126
display id: annotation2187126
version: 1
top level: BBa_K777108
location: https://synbiohub.org/public/igem/BBa_K777108/annotation2187126/range2187126/1
component: BBa_K777100

https://synbiohub.org/public/igem/BBa_K777108/annotation2187126/range2187126/1
type: Range
persistent identity: range2187126
display id: range2187126
version: 1
direction: direction/reverse
top level: BBa_K777108
start: 42
end: 910
orientation: inline

BBa_I712004
type: ComponentDefinition
persistent identity: BBa I712004
display id: BBa_I712004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CMV promoter
creator: Andrej Ondracka
created: 2007-10-18T11:00:00Z
modified: 2015-08-31T04:07:45Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _130_
m user id: 0
owner id: 1896
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: a constitutive expression promoter or use in mammalian cells. Ribosome binding site is included.
mutable notes: Cloned into pSB1AC3 vector.
mutable provenance: pcDNA3 by Invitrogen
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I712004_sequence/1

BBa_B0039
type: ComponentDefinition
persistent identity: BBa B0039
display id: BBa_B0039
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS 2
creator: Josh Michener
created: 2005-12-20T12:00:00Z
modified: 2015-08-31T04:07:20Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_
m user id: 0
owner id: 253
owning group id: 6
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Modified version of B0031 from the Voigt Lab. Not currently available as an independent part (only in a tet inverter).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_B0039_sequence/1

BBa_J61140
type: ComponentDefinition
persistent identity: BBa J61140
display id: BBa_J61140
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: {rbs1} Library Member in BBb
creator: John Anderson
created: 2007-09-03T11:00:00Z
modified: 2015-08-31T01:59:49Z
discontinued: false
dominant: false
experience: experience/None
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: {{BBbRBS1Family}}
mutable notes: {{BerkiGEM2007-Biobrick2.0Intro}}
mutable provenance: Synthetic
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J61140_sequence/1

BBa_B2102
type: ComponentDefinition
persistent identity: BBa B2102
display id: BBa_B2102
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: T7 RBS 0.4 + SapI (rev)
creator: Heather Keller
created: 2006-09-27T11:00:00Z
modified: 2015-08-31T04:07:21Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_
m user id: 0
owner id: 571
owning group id: 10
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Insert Text here
mutable notes: Insert Text here
mutable provenance: Insert Text here
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: SapI
sequence annotation: BBa_B2002
sequence annotation: BBa_B0079
sequence annotation: SD region
sequence: https://synbiohub.org/public/igem/BBa_B2102_sequence/1

SapI
type: SequenceAnnotation
persistent identity: annotation1902502
display id: annotation1902502
version: 1
top level: BBa_B2102
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_B2102/annotation1902502/range1902502/1

https://synbiohub.org/public/igem/BBa_B2102/annotation1902502/range1902502/1
type: Range
persistent identity: range1902502
display id: range1902502
version: 1
direction: direction/reverse
top level: BBa_B2102
start: 25
end: 31
orientation: inline

BBa_B2002
type: SequenceAnnotation
persistent identity: annotation1902499
display id: annotation1902499
version: 1
top level: BBa_B2102
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_B2102/annotation1902499/range1902499/1

https://synbiohub.org/public/igem/BBa_B2102/annotation1902499/range1902499/1
type: Range
persistent identity: range1902499
display id: range1902499
version: 1
direction: direction/forward
top level: BBa_B2102
start: 1
end: 20
orientation: inline

BBa_B0079
type: SequenceAnnotation
persistent identity: annotation1902501
display id: annotation1902501
version: 1
top level: BBa_B2102
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_B2102/annotation1902501/range1902501/1

https://synbiohub.org/public/igem/BBa_B2102/annotation1902501/range1902501/1
type: Range
persistent identity: range1902501
display id: range1902501
version: 1
direction: direction/forward
top level: BBa_B2102
start: 21
end: 32
orientation: inline

SD region
type: SequenceAnnotation
persistent identity: annotation1902500
display id: annotation1902500
version: 1
top level: BBa_B2102
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_B2102/annotation1902500/range1902500/1

https://synbiohub.org/public/igem/BBa_B2102/annotation1902500/range1902500/1
type: Range
persistent identity: range1902500
display id: range1902500
version: 1
direction: direction/forward
top level: BBa_B2102
start: 4
end: 10
orientation: inline

BBa_K777006
type: ComponentDefinition
persistent identity: BBa K777006
display id: BBa_K777006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Tar receptor under the control of constitutive promoter J23112
creator: Bianca Genenncher
created: 2012-09-20T11:00:00Z
modified: 2015-05-08T01:13:16Z
discontinued: false
dominant: false
group u list: _1029_
m user id: 0
owner id: 11946
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: The transmembrane chemoreceptor Tar of E. coli mediates chemotaxis towards aspartate and exists as a functional homodimer. Here we used a set of 8 constitutive [http://partsregistry.org/Part:BBa_J23112 Anderson promoters] from the 2006 Berkeley group to test how different levels of constitutive ''Tar'' expression affect the chemotaxis of ''E. coli''.
mutable notes: * Genomic sequence was amplified using the following primers. ** Primers PCR: Base pairs in caps represent the respective restriction sites followed by either prefix or suffix. Primers were provided by SIGMA. *** Fwd: Tar for + prefix: 5?? actGAATTCgcggccgctTCTAGAtgattaaccgtatccgcgtagtc 3?? *** Rev: Tar rev + suffix: 5?? tcCTGCAGcggccgctACTAGTcaaaatgtttcccagtttggatc 3?? * One XbaI site was found at position 420. The restriction site was changed from TCTAGt to CCTAGt via QuikChange PCR. ** Primers QuikChange (QC): Primers were provided by SIGMA. *** Fwd: TarQC for: 5?? ACTGATTGATTAcCTAGATTATGGCAATACTGGAG 3?? *** Rev: TarQC rev: 5?? TGCCATAATCTAGgTAATCAATCAGTTCAGTTAAC 3?? (the uncapitalized letter induces the mutation for removal of the XbaI site)
mutable provenance: *The part was amplified from genomic DNA of ''E. coli'' str. K-12 substr. DH10B, complete genome (CP000948.1). *J23112 information was taken from the partsregistry and physical DNA from the 2012 distribution kit.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_J23112
component: BBa_K777000
sequence annotation: BBa_J23112
sequence annotation: BBa_K777000
sequence: https://synbiohub.org/public/igem/BBa_K777006_sequence/1

BBa_J23112
type: Component
persistent identity: component2189394
display id: component2189394
version: 1
top level: BBa_K777006
access: public
definition: BBa_J23112

BBa_K777000
type: Component
persistent identity: component2189398
display id: component2189398
version: 1
top level: BBa_K777006
access: public
definition: BBa_K777000

BBa_J23112
type: SequenceAnnotation
persistent identity: annotation2189394
display id: annotation2189394
version: 1
top level: BBa_K777006
location: https://synbiohub.org/public/igem/BBa_K777006/annotation2189394/range2189394/1
component: BBa_J23112

https://synbiohub.org/public/igem/BBa_K777006/annotation2189394/range2189394/1
type: Range
persistent identity: range2189394
display id: range2189394
version: 1
direction: direction/reverse
top level: BBa_K777006
start: 1
end: 35
orientation: inline

BBa_K777000
type: SequenceAnnotation
persistent identity: annotation2189398
display id: annotation2189398
version: 1
top level: BBa_K777006
location: https://synbiohub.org/public/igem/BBa_K777006/annotation2189398/range2189398/1
component: BBa_K777000

https://synbiohub.org/public/igem/BBa_K777006/annotation2189398/range2189398/1
type: Range
persistent identity: range2189398
display id: range2189398
version: 1
direction: direction/reverse
top level: BBa_K777006
start: 42
end: 1703
orientation: inline

BBa_B0035
type: ComponentDefinition
persistent identity: BBa B0035
display id: BBa_B0035
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS (B0030 derivative)
creator: Jason Kelly
created: 2004-01-27T12:00:00Z
modified: 2015-08-31T04:07:20Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_B0035_sequence/1

BBa_J61124
type: ComponentDefinition
persistent identity: BBa J61124
display id: BBa_J61124
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ribosome Binding Site Family Member
creator: John Anderson
created: 2007-04-24T11:00:00Z
modified: 2015-08-31T01:59:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable description: {{JCA_Arkin_RBSFamily}}
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J61124_sequence/1

BBa_J64808
type: ComponentDefinition
persistent identity: BBa J64808
display id: BBa_J64808
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RocE RBS
creator: David Stanley
created: 2007-03-19T12:00:00Z
modified: 2015-05-08T01:08:17Z
discontinued: false
dominant: false
experience: experience/None
group u list: _98_
m user id: 0
owner id: 1423
owning group id: 98
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: RBS preceding RocE
mutable notes: n
mutable provenance: n
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J64808_sequence/1

T7 0.3
type: ComponentDefinition
persistent identity: BBa B2001
display id: BBa_B2001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: T7 RBS 0.3
creator: Heather Keller
created: 2006-09-26T11:00:00Z
modified: 2015-08-31T04:07:21Z
discontinued: false
dominant: true
experience: experience/None
group u list: _11_
m user id: 0
owner id: 571
owning group id: 10
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Test RBS from T7 that may be used in E.coli
mutable notes: None
mutable provenance: T7+ genome RBS upstream of gene 0.3
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: SD region
sequence: https://synbiohub.org/public/igem/BBa_B2001_sequence/1

SD region
type: SequenceAnnotation
persistent identity: annotation1902503
display id: annotation1902503
version: 1
top level: T7 0.3
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_B2001/annotation1902503/range1902503/1

https://synbiohub.org/public/igem/BBa_B2001/annotation1902503/range1902503/1
type: Range
persistent identity: range1902503
display id: range1902503
version: 1
direction: direction/forward
top level: T7 0.3
start: 8
end: 12
orientation: inline

BBa_J61131
type: ComponentDefinition
persistent identity: BBa J61131
display id: BBa_J61131
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ribosome Binding Site Family Member
creator: John Anderson
created: 2007-04-24T11:00:00Z
modified: 2015-08-31T01:59:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable description: {{JCA_Arkin_RBSFamily}}
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J61131_sequence/1

BBa_J23117
type: ComponentDefinition
persistent identity: BBa J23117
display id: BBa_J23117
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: constitutive promoter family member
creator: John Anderson
created: 2006-08-16T11:00:00Z
modified: 2015-08-31T04:08:40Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: Later
mutable notes: N/A
mutable provenance: Later
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J23117_sequence/1

BBa_K124000
type: ComponentDefinition
persistent identity: BBa K124000
display id: BBa_K124000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pCYC Yeast Promoter
creator: John Szymanski
created: 2008-06-30T11:00:00Z
modified: 2015-05-08T01:09:44Z
discontinued: false
dominant: false
group u list: _221_
m user id: 0
owner id: 2512
owning group id: 58
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: The promoter from the yeast gene CYC1. Activity characterized here: http://dx.doi.org/10.1016/0378-1119(95)00037-7.
mutable notes: none
mutable provenance: From Mumberg, Muller, and Funk p413 pCYC. See ref. above
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K124000_sequence/1

BBa_B0078
type: ComponentDefinition
persistent identity: BBa B0078
display id: BBa_B0078
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Specialized RBS
creator: Bartholomew Canton
created: 2006-05-01T11:00:00Z
modified: 2015-08-31T04:07:21Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_6_
m user id: 0
owner id: 135
owning group id: 6
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_B0078_sequence/1

BBa_J23114
type: ComponentDefinition
persistent identity: BBa J23114
display id: BBa_J23114
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: constitutive promoter family member
creator: John Anderson
created: 2006-08-16T11:00:00Z
modified: 2015-08-31T04:08:40Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: Later
mutable notes: N/A
mutable provenance: Later
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J23114_sequence/1

BBa_J61115
type: ComponentDefinition
persistent identity: BBa J61115
display id: BBa_J61115
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ribosome Binding Site Family Member
creator: John Anderson
created: 2007-04-23T11:00:00Z
modified: 2015-08-31T02:03:00Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable description: {{JCA_Arkin_RBSFamily}}
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J61115_sequence/1

CP1LacPI2
type: ComponentDefinition
persistent identity: BBa K418002
display id: BBa_K418002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: IPTG inducible Lac promoter cassette
creator: Northwestern University iGEM 2010
created: 2010-10-15T11:00:00Z
modified: 2015-05-08T01:12:28Z
discontinued: false
dominant: false
experience: experience/None
group u list: _532_
m user id: 0
owner id: 6938
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_J23100
component: BBa_Q04121
sequence annotation: BBa_Q04121
sequence annotation: BBa_J23100
sequence: https://synbiohub.org/public/igem/BBa_K418002_sequence/1

BBa_J23100
type: Component
persistent identity: component2263743
display id: component2263743
version: 1
top level: CP1LacPI2
access: public
definition: BBa_J23100

BBa_Q04121
type: Component
persistent identity: component2263762
display id: component2263762
version: 1
top level: CP1LacPI2
access: public
definition: https://synbiohub.org/public/igem/BBa_Q04121/1

BBa_Q04121
type: SequenceAnnotation
persistent identity: annotation2263762
display id: annotation2263762
version: 1
top level: CP1LacPI2
location: https://synbiohub.org/public/igem/BBa_K418002/annotation2263762/range2263762/1
component: BBa_Q04121

https://synbiohub.org/public/igem/BBa_K418002/annotation2263762/range2263762/1
type: Range
persistent identity: range2263762
display id: range2263762
version: 1
direction: direction/reverse
top level: CP1LacPI2
start: 44
end: 1414
orientation: inline

BBa_J23100
type: SequenceAnnotation
persistent identity: annotation2263743
display id: annotation2263743
version: 1
top level: CP1LacPI2
location: https://synbiohub.org/public/igem/BBa_K418002/annotation2263743/range2263743/1
component: BBa_J23100

https://synbiohub.org/public/igem/BBa_K418002/annotation2263743/range2263743/1
type: Range
persistent identity: range2263743
display id: range2263743
version: 1
direction: direction/reverse
top level: CP1LacPI2
start: 1
end: 35
orientation: inline

BBa_K780003
type: ComponentDefinition
persistent identity: BBa K780003
display id: BBa_K780003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Strong constitutive promoter for Bacillus subtilis
creator: Elżbieta Jankowska
created: 2012-09-25T11:00:00Z
modified: 2015-05-08T01:13:18Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1034_
m user id: 0
owner id: 10113
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Strong constitutive promoter for Bacillus subtilis
mutable notes: -
mutable provenance: 'host-takover module' from B.subtilis Bacteriophage SPO1
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K780003_sequence/1

BBa_M13509
type: ComponentDefinition
persistent identity: BBa M13509
display id: BBa_M13509
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: M13Ko7 gene IX RBS
creator: Natalie Kuldell
created: 2006-12-14T12:00:00Z
modified: 2015-05-08T01:13:56Z
discontinued: false
dominant: false
experience: experience/None
group u list: _45_
m user id: 0
owner id: 314
owning group id: 1
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This RBS is part of the bacteriophage M13 genome, initiating translation of the gene IX message (BBa_M13009). The part aligns with base pairs 1190-1205 in M13K07 genome. It was identified as the RBS for gene IX message by Wezenbeek et al in Gene (1980) 11: 129-148
mutable notes: none
mutable provenance: New England Biolabs M13K07. Sequenced in 2002 by L. Panganaban
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_M13509_sequence/1

BBa_B0064
type: ComponentDefinition
persistent identity: BBa B0064
display id: BBa_B0064
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Tuned RBS for Q04401
creator: Jason Kelly
created: 2008-02-23T12:00:00Z
modified: 2015-08-31T04:07:21Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_
m user id: 0
owner id: 2
owning group id: 84
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is a single bp change from B0034. It's strength is approximately 35% that of B0034.
mutable notes: .
mutable provenance: .
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_B0064_sequence/1

BBa_R0181
type: ComponentDefinition
persistent identity: BBa R0181
display id: BBa_R0181
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: T7 RNAP promoter
creator: Barry Canton
created: 2005-09-25T11:00:00Z
modified: 2015-05-08T01:14:15Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_6_
m user id: 0
owner id: 135
owning group id: 6
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: T7 promoter with an T->G mutation at the -17 position of the consensus sequence
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_R0181_sequence/1

BBa_J61127
type: ComponentDefinition
persistent identity: BBa J61127
display id: BBa_J61127
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ribosome Binding Site Family Member
creator: John Anderson
created: 2007-04-24T11:00:00Z
modified: 2015-08-31T01:59:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable description: {{JCA_Arkin_RBSFamily}}
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J61127_sequence/1

BBa_B0037
type: ComponentDefinition
persistent identity: BBa B0037
display id: BBa_B0037
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Specialized RBS
creator: Barry Canton
created: 2005-12-06T12:00:00Z
modified: 2015-08-31T04:07:20Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_6_
m user id: 0
owner id: 135
owning group id: 6
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: RBS specific to dedicated ribosomes. This RBS is a modified version of B0036. The spacing between the SD sequence and the start codon has been increased by 5 bases to give the same spacing as used by Brink et al. The 5 bases are the same as those used by Brink. That means they form the first five bases of an XbaI site and no negative affects of using these bases are known at present.
mutable provenance: Brink MF, Verbeet MP, de Boer HA (1995). Specialized ribosomes: highly specific translation in vivo of a single targetted mRNA species. Gene, 156, pp. 215-222
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_B0037_sequence/1

BBa_K777005
type: ComponentDefinition
persistent identity: BBa K777005
display id: BBa_K777005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Tar receptor under the control of constitutive promoter J23109
creator: Bianca Genenncher
created: 2012-09-20T11:00:00Z
modified: 2015-05-08T01:13:16Z
discontinued: false
dominant: false
group u list: _1029_
m user id: 0
owner id: 11946
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: The transmembrane chemoreceptor Tar of E. coli mediates chemotaxis towards aspartate and exists as a functional homodimer. Here we used a set of 8 constitutive [http://partsregistry.org/Part:BBa_J23109 Anderson promoters] from the 2006 Berkeley group to test how different levels of constitutive ''Tar'' expression affect the chemotaxis of ''E. coli''.
mutable notes: * Genomic sequence was amplified using the following primers. ** Primers PCR: Base pairs in caps represent the respective restriction sites followed by either prefix or suffix. Primers were provided by SIGMA. *** Fwd: Tar for + prefix: 5?? actGAATTCgcggccgctTCTAGAtgattaaccgtatccgcgtagtc 3?? *** Rev: Tar rev + suffix: 5?? tcCTGCAGcggccgctACTAGTcaaaatgtttcccagtttggatc 3?? * One XbaI site was found at position 420. The restriction site was changed from TCTAGt to CCTAGt via QuikChange PCR. ** Primers QuikChange (QC): Primers were provided by SIGMA. *** Fwd: TarQC for: 5?? ACTGATTGATTAcCTAGATTATGGCAATACTGGAG 3?? *** Rev: TarQC rev: 5?? TGCCATAATCTAGgTAATCAATCAGTTCAGTTAAC 3?? (the uncapitalized letter induces the mutation for removal of the XbaI site)
mutable provenance: *The part was amplified from genomic DNA of ''E. coli'' str. K-12 substr. DH10B, complete genome (CP000948.1). *J23109 information was taken from the partsregistry and physical DNA from the 2012 distribution kit.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_J23109
component: BBa_K777000
sequence annotation: BBa_J23109
sequence annotation: BBa_K777000
sequence: https://synbiohub.org/public/igem/BBa_K777005_sequence/1

BBa_J23109
type: Component
persistent identity: component2189368
display id: component2189368
version: 1
top level: BBa_K777005
access: public
definition: BBa_J23109

BBa_K777000
type: Component
persistent identity: component2189372
display id: component2189372
version: 1
top level: BBa_K777005
access: public
definition: BBa_K777000

BBa_J23109
type: SequenceAnnotation
persistent identity: annotation2189368
display id: annotation2189368
version: 1
top level: BBa_K777005
location: https://synbiohub.org/public/igem/BBa_K777005/annotation2189368/range2189368/1
component: BBa_J23109

https://synbiohub.org/public/igem/BBa_K777005/annotation2189368/range2189368/1
type: Range
persistent identity: range2189368
display id: range2189368
version: 1
direction: direction/reverse
top level: BBa_K777005
start: 1
end: 35
orientation: inline

BBa_K777000
type: SequenceAnnotation
persistent identity: annotation2189372
display id: annotation2189372
version: 1
top level: BBa_K777005
location: https://synbiohub.org/public/igem/BBa_K777005/annotation2189372/range2189372/1
component: BBa_K777000

https://synbiohub.org/public/igem/BBa_K777005/annotation2189372/range2189372/1
type: Range
persistent identity: range2189372
display id: range2189372
version: 1
direction: direction/reverse
top level: BBa_K777005
start: 42
end: 1703
orientation: inline

BBa_M13102
type: ComponentDefinition
persistent identity: BBa M13102
display id: BBa_M13102
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: M13K07 gene II promoter
creator: Natalie Kuldell
created: 2006-12-15T12:00:00Z
modified: 2015-05-08T01:13:55Z
discontinued: false
dominant: true
experience: experience/None
group u list: _45_
m user id: 0
owner id: 314
owning group id: 1
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This part is from the bacteriophage M13 genome. It directs transcription of M13 gene II (BBa_M13502, BBa_M13002). Promoter identified in Gene (1980) 11:129-148 based on -10,-35 homology and fragment of genome able to bind RNAP.
mutable notes: none
mutable provenance: New England Biolabs M13K07. Sequenced in 2002 by L. Panganaban.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_M13102_sequence/1

BBa_I11010
type: ComponentDefinition
persistent identity: BBa I11010
display id: BBa_I11010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS
creator: bu
created: 2004-09-02T11:00:00Z
modified: 2015-08-31T04:07:30Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2_
m user id: 0
owner id: 102
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: ribosome binding site
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_I11010_sequence/1

RBS
type: SequenceAnnotation
persistent identity: annotation1147662
display id: annotation1147662
version: 1
top level: BBa_I11010
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_I11010/annotation1147662/range1147662/1

https://synbiohub.org/public/igem/BBa_I11010/annotation1147662/range1147662/1
type: Range
persistent identity: range1147662
display id: range1147662
version: 1
direction: direction/forward
top level: BBa_I11010
start: 1
end: 6
orientation: inline

BBa_K283012
type: ComponentDefinition
persistent identity: BBa K283012
display id: BBa_K283012
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pT7-rbs
creator: W Ling , Shi Lei, Nelson Chu
created: 2009-10-12T11:00:00Z
modified: 2015-05-08T01:11:47Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _383_
m user id: 0
owner id: 5494
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: pT7 promoter with a rbs
mutable notes: BBa_K283012
mutable provenance: From the registry
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_I712074
component: BBa_B0034
sequence annotation: BBa_B0034
sequence annotation: BBa_I712074
sequence: https://synbiohub.org/public/igem/BBa_K283012_sequence/1

BBa_I712074
type: Component
persistent identity: component2035622
display id: component2035622
version: 1
top level: BBa_K283012
access: public
definition: BBa_I712074

BBa_B0034
type: Component
persistent identity: component2035624
display id: component2035624
version: 1
top level: BBa_K283012
access: public
definition: BBa_B0034

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2035624
display id: annotation2035624
version: 1
top level: BBa_K283012
location: https://synbiohub.org/public/igem/BBa_K283012/annotation2035624/range2035624/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K283012/annotation2035624/range2035624/1
type: Range
persistent identity: range2035624
display id: range2035624
version: 1
direction: direction/reverse
top level: BBa_K283012
start: 55
end: 66
orientation: inline

BBa_I712074
type: SequenceAnnotation
persistent identity: annotation2035622
display id: annotation2035622
version: 1
top level: BBa_K283012
location: https://synbiohub.org/public/igem/BBa_K283012/annotation2035622/range2035622/1
component: BBa_I712074

https://synbiohub.org/public/igem/BBa_K283012/annotation2035622/range2035622/1
type: Range
persistent identity: range2035622
display id: range2035622
version: 1
direction: direction/reverse
top level: BBa_K283012
start: 1
end: 46
orientation: inline

BBa_J23104
type: ComponentDefinition
persistent identity: BBa J23104
display id: BBa_J23104
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: constitutive promoter family member
creator: John Anderson
created: 2006-08-03T11:00:00Z
modified: 2015-08-31T04:08:40Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: true
mutable description: replace later
mutable notes: N/A
mutable provenance: isolated from library of promoters
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J23104_sequence/1

BBa_J23150
type: ComponentDefinition
persistent identity: BBa J23150
display id: BBa_J23150
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: 1bp mutant from J23107
creator: Jason Kelly
created: 2008-02-11T12:00:00Z
modified: 2015-08-31T04:08:41Z
discontinued: false
dominant: true
group u list: _11_
m user id: 0
owner id: 2
owning group id: 84
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: .
mutable notes: .
mutable provenance: ..
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J23150_sequence/1

BBa_R0182
type: ComponentDefinition
persistent identity: BBa R0182
display id: BBa_R0182
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: T7 RNAP promoter
creator: Barry Canton
created: 2005-09-25T11:00:00Z
modified: 2015-05-08T01:14:15Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_6_
m user id: 0
owner id: 135
owning group id: 6
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: T7 promoter with an A->T mutation at the -10 position of the consensus sequence
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_R0182_sequence/1

BBa_I742159
type: ComponentDefinition
persistent identity: BBa I742159
display id: BBa_I742159
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: crtI native rbs
creator: Chris French
created: 2007-10-23T11:00:00Z
modified: 2015-08-31T04:08:03Z
discontinued: false
dominant: false
experience: experience/None
group u list: _123_
m user id: 0
owner id: 837
owning group id: 163
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Native rbs from crtI (phytoene dehydrogenase) of Pantoea ananatis (formerly Erwinia uredovora) DSMZ 30080 (ATCC 19321). Accession: D90087.
mutable notes: Virtual part to be added to the coding sequence.
mutable provenance: Pantoea ananatis (formerly Erwinia uredovora) DSMZ 30080 (ATCC 19321). Accession: D90087.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: rbs
sequence: https://synbiohub.org/public/igem/BBa_I742159_sequence/1

rbs
type: SequenceAnnotation
persistent identity: annotation1953816
display id: annotation1953816
version: 1
top level: BBa_I742159
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_I742159/annotation1953816/range1953816/1

https://synbiohub.org/public/igem/BBa_I742159/annotation1953816/range1953816/1
type: Range
persistent identity: range1953816
display id: range1953816
version: 1
direction: direction/forward
top level: BBa_I742159
start: 10
end: 15
orientation: inline

BBa_K1585117
type: ComponentDefinition
persistent identity: BBa K1585117
display id: BBa_K1585117
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Anderson Promoter with lacI binding site
creator: Tobias Schwanemann
created: 2015-08-31T11:00:00Z
modified: 2015-09-01T02:23:31Z
discontinued: false
dominant: false
experience: experience/Fails
group u list: _2002_
m user id: 24474
owner id: 24474
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Promoters of the Anderson Promoter Collection with lacI binding site derived from [http://parts.igem.org/Part:BBa_R0010 R_0010] to make them [http://openwetware.org/wiki/IPTG IPTG] iducible.
mutable notes: We choosed lacI-binding-site of part BBa_R0010 because we assumed older parts would be more reliable. The part is shipped in backbone [http://parts.igem.org/Part:BBa_J61002 J61002] because the functionality is directly visible by adding IPTG. RFP ([http://parts.igem.org/Part:BBa_E1010 E1010]) should get expressed then.
mutable provenance: Assambly of [http://parts.igem.org/Part:BBa_J23116 J23116] and lacI-binding-site of [http://parts.igem.org/Part:BBa_R0010 R_0010] The "lacI binding site" of Part [http://parts.igem.org/Part:BBa_R0010 R_0010] differs from lacO from Part [http://parts.igem.org/Part:BBa_J33207 BBa_J33207].
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
component: BBa_K1585999
component: BBa_J23117
sequence annotation: BBa_J23117
sequence annotation: BBa_K1585999
sequence: https://synbiohub.org/public/igem/BBa_K1585117_sequence/1

BBa_K1585999
type: Component
persistent identity: component2441713
display id: component2441713
version: 1
top level: BBa_K1585117
access: public
definition: https://synbiohub.org/public/igem/BBa_K1585999/1

BBa_J23117
type: Component
persistent identity: component2441711
display id: component2441711
version: 1
top level: BBa_K1585117
access: public
definition: BBa_J23117

BBa_J23117
type: SequenceAnnotation
persistent identity: annotation2441711
display id: annotation2441711
version: 1
top level: BBa_K1585117
location: https://synbiohub.org/public/igem/BBa_K1585117/annotation2441711/range2441711/1
component: BBa_J23117

https://synbiohub.org/public/igem/BBa_K1585117/annotation2441711/range2441711/1
type: Range
persistent identity: range2441711
display id: range2441711
version: 1
direction: direction/reverse
top level: BBa_K1585117
start: 1
end: 35
orientation: inline

BBa_K1585999
type: SequenceAnnotation
persistent identity: annotation2441713
display id: annotation2441713
version: 1
top level: BBa_K1585117
location: https://synbiohub.org/public/igem/BBa_K1585117/annotation2441713/range2441713/1
component: BBa_K1585999

https://synbiohub.org/public/igem/BBa_K1585117/annotation2441713/range2441713/1
type: Range
persistent identity: range2441713
display id: range2441713
version: 1
direction: direction/reverse
top level: BBa_K1585117
start: 44
end: 78
orientation: inline

creC RBS
type: ComponentDefinition
persistent identity: BBa J64908
display id: BBa_J64908
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS for creC in e coli operon
creator: Ryan Ritterson
created: 2007-03-19T12:00:00Z
modified: 2015-05-08T01:08:17Z
discontinued: false
dominant: false
experience: experience/None
group u list: _98_
m user id: 0
owner id: 1422
owning group id: 98
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: The ribosomal binding site for gene creC in the E Coli cre operon.
mutable notes: natural sequence, no design
mutable provenance: E Coli K12 strain.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J64908_sequence/1

BBa_K076017
type: ComponentDefinition
persistent identity: BBa K076017
display id: BBa_K076017
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ubc Promoter
creator: Available
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:08:32Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _215_
m user id: 0
owner id: 3466
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is a stable constitutive promoter.
mutable notes: Using this promoter allows for the constant expression of a reporter gene or a selectable antibiotic.
mutable provenance: This part was obtained from members of the Weiss Lab.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K076017_sequence/1

p(osmY)
type: ComponentDefinition
persistent identity: BBa J45992
display id: BBa_J45992
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Full-length stationary phase osmY promoter
creator: Stephen Payne
created: 2006-07-10T11:00:00Z
modified: 2015-08-31T04:08:50Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _84_
m user id: 0
owner id: 642
owning group id: 84
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: osmY's promoter is active in stationary phase and under high osmotic pressure conditions.
mutable notes: Truncated Forward Primer: 5'- GTT TCT TCG AAT TCG CGG CCG CTT CTA GGCT TAT GTT TTC GCT GAT ATC - 3' Total Length: 49 bp Annealing Length: 21 bp GC Content: 38.1% Melting Temperature: 49.5 degrees C hairpin deltaG: -2.66 kcal/mol self dimer deltaG: -99.97 kcal/mol Full Forward Primer: 5'- GTT TCT TCG AAT TCG CGG CCG CTT CTA GCTG GCA CAG GAA CGT TAT C - 3' Total Length: 47 bp Annealing Length: 19 bp GC Content: 52.6% Melting Temperature: 53.4 degrees C hairpin deltaG: -3.99 kcal/mol self dimer deltaG: -98.3 kcal/mol Reverse Primer: 5'-GTT TCT TCC TGC AGC GGC CGC TAC TAG TAT TGT TAA ATA TAG ATC ACA ATT TTG- 3' Total Length: 54 bp Annealing Length: 25 bp GC Content: 20.0% Melting Temperature: 46.4 degrees C hairpin deltaG: -2.86 kcal/mol self dimer deltaG: -99.44 kcal/mol heterodimer deltaG with Forward Primer: -99.44 kcal/mol heterodimer deltaG with Conserved Forward Primer: -99.97 kcal/mol
mutable provenance: This part was PCRed out of E. coli's genome.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -10
sequence annotation: -35
sequence: https://synbiohub.org/public/igem/BBa_J45992_sequence/1

-10
type: SequenceAnnotation
persistent identity: annotation1883645
display id: annotation1883645
version: 1
top level: p(osmY)
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J45992/annotation1883645/range1883645/1

https://synbiohub.org/public/igem/BBa_J45992/annotation1883645/range1883645/1
type: Range
persistent identity: range1883645
display id: range1883645
version: 1
direction: direction/forward
top level: p(osmY)
start: 188
end: 193
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1883646
display id: annotation1883646
version: 1
top level: p(osmY)
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J45992/annotation1883646/range1883646/1

https://synbiohub.org/public/igem/BBa_J45992/annotation1883646/range1883646/1
type: Range
persistent identity: range1883646
display id: range1883646
version: 1
direction: direction/forward
top level: p(osmY)
start: 164
end: 169
orientation: inline

BBa_J64809
type: ComponentDefinition
persistent identity: BBa J64809
display id: BBa_J64809
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RocF RBS
creator: David Stanley
created: 2007-03-19T12:00:00Z
modified: 2015-05-08T01:08:17Z
discontinued: false
dominant: false
experience: experience/None
group u list: _98_
m user id: 0
owner id: 1423
owning group id: 98
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: RBS upstream of RocF
mutable notes: n
mutable provenance: n
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J64809_sequence/1

LacZ promo
type: ComponentDefinition
persistent identity: BBa K119000
display id: BBa_K119000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Constitutive weak promoter of lacZ
creator: Miguel Angel Ramirez-Romero
created: 2008-09-27T11:00:00Z
modified: 2015-05-08T01:09:38Z
discontinued: false
dominant: false
experience: experience/None
group u list: _235_
m user id: 0
owner id: 3054
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: LacZ constitutive promoter, without including operators.
mutable notes: We needed two versions of it.
mutable provenance: Lac operon from E. coli.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: LacZ promoter
sequence: https://synbiohub.org/public/igem/BBa_K119000_sequence/1

LacZ promoter
type: SequenceAnnotation
persistent identity: annotation1976843
display id: annotation1976843
version: 1
top level: LacZ promo
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K119000/annotation1976843/range1976843/1

https://synbiohub.org/public/igem/BBa_K119000/annotation1976843/range1976843/1
type: Range
persistent identity: range1976843
display id: range1976843
version: 1
direction: direction/forward
top level: LacZ promo
start: 1
end: 38
orientation: inline

BBa_J61112
type: ComponentDefinition
persistent identity: BBa J61112
display id: BBa_J61112
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ribosome Binding Site Family Member
creator: John Anderson
created: 2007-04-23T11:00:00Z
modified: 2015-08-31T02:03:00Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable description: {{JCA_Arkin_RBSFamily}}
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J61112_sequence/1

BBa_I766555
type: ComponentDefinition
persistent identity: BBa I766555
display id: BBa_I766555
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pCyc (Medium) Promoter
creator: Nili Sommovilla
created: 2007-08-15T11:00:00Z
modified: 2015-08-31T04:08:10Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _155_
m user id: 0
owner id: 1931
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Mid-expression level constitutive promoter in yeast
mutable notes: none
mutable provenance: Endogenous promoter for Cytochrome c
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I766555_sequence/1

LacZ prom
type: ComponentDefinition
persistent identity: BBa K119001
display id: BBa_K119001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Mutated LacZ promoter
creator: Miguel Angel Ram??rez-Romero
created: 2008-09-27T11:00:00Z
modified: 2015-05-08T01:09:38Z
discontinued: false
dominant: false
experience: experience/None
group u list: _235_
m user id: 0
owner id: 3054
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Constitutive weak promoter of lacZ which sequence was changed at position 29 (T->G) in order to make it weaker
mutable notes: We needed two version of it.
mutable provenance: Lac Operon from E. coli.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: LacZ prom mut
sequence annotation: s_mutation
sequence: https://synbiohub.org/public/igem/BBa_K119001_sequence/1

LacZ prom mut
type: SequenceAnnotation
persistent identity: annotation1976842
display id: annotation1976842
version: 1
top level: LacZ prom
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K119001/annotation1976842/range1976842/1

https://synbiohub.org/public/igem/BBa_K119001/annotation1976842/range1976842/1
type: Range
persistent identity: range1976842
display id: range1976842
version: 1
direction: direction/forward
top level: LacZ prom
start: 1
end: 38
orientation: inline

s_mutation
type: SequenceAnnotation
persistent identity: annotation1977487
display id: annotation1977487
version: 1
top level: LacZ prom
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_K119001/annotation1977487/range1977487/1

https://synbiohub.org/public/igem/BBa_K119001/annotation1977487/range1977487/1
type: Range
persistent identity: range1977487
display id: range1977487
version: 1
direction: direction/forward
top level: LacZ prom
start: 29
end: 29
orientation: inline

BBa_J23111
type: ComponentDefinition
persistent identity: BBa J23111
display id: BBa_J23111
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: constitutive promoter family member
creator: John Anderson
created: 2006-08-16T11:00:00Z
modified: 2015-08-31T04:08:40Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: true
mutable description: Later
mutable notes: N/A
mutable provenance: Later
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J23111_sequence/1

BBa_M13110
type: ComponentDefinition
persistent identity: BBa M13110
display id: BBa_M13110
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: M13110
creator: Natalie Kuldell
created: 2006-12-15T12:00:00Z
modified: 2015-05-08T01:13:55Z
discontinued: false
dominant: false
group u list: _45_
m user id: 0
owner id: 314
owning group id: 1
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This part is from the bacteriophage M13 genome, bp 381-428 in M13K07. It directs transcription of M13 gene X (BBa_M13510, BBa_M13010). Its identity as a promoter was described in Gene (1980) 11:129-148 based on -10,-35 homology and fragment of genome able to bind RNAP.
mutable notes: none
mutable provenance: New England Biolabs M13K07. Sequenced in 2002 by L. Panganaban.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_M13110_sequence/1

BBa_K256018
type: ComponentDefinition
persistent identity: BBa K256018
display id: BBa_K256018
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: J23119:IFP
creator: iGEM09_NTU-Singapore
created: 2009-10-16T11:00:00Z
modified: 2015-05-08T01:11:41Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _359_
m user id: 0
owner id: 4864
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: IFP Generator
mutable notes: --
mutable provenance: --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0012
component: BBa_B0010
component: BBa_J23119
component: BBa_B0034
component: BBa_K256008
sequence annotation: BBa_B0034
sequence annotation: BBa_B0010
sequence annotation: BBa_J23119
sequence annotation: BBa_B0012
sequence annotation: BBa_K256008
sequence: https://synbiohub.org/public/igem/BBa_K256018_sequence/1

BBa_B0012
type: Component
persistent identity: component2048514
display id: component2048514
version: 1
top level: BBa_K256018
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0010
type: Component
persistent identity: component2048512
display id: component2048512
version: 1
top level: BBa_K256018
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_J23119
type: Component
persistent identity: component2048508
display id: component2048508
version: 1
top level: BBa_K256018
access: public
definition: BBa_J23119

BBa_B0034
type: Component
persistent identity: component2048510
display id: component2048510
version: 1
top level: BBa_K256018
access: public
definition: BBa_B0034

BBa_K256008
type: Component
persistent identity: component2048511
display id: component2048511
version: 1
top level: BBa_K256018
access: public
definition: https://synbiohub.org/public/igem/BBa_K256008/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2048510
display id: annotation2048510
version: 1
top level: BBa_K256018
location: https://synbiohub.org/public/igem/BBa_K256018/annotation2048510/range2048510/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K256018/annotation2048510/range2048510/1
type: Range
persistent identity: range2048510
display id: range2048510
version: 1
direction: direction/reverse
top level: BBa_K256018
start: 44
end: 55
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2048512
display id: annotation2048512
version: 1
top level: BBa_K256018
location: https://synbiohub.org/public/igem/BBa_K256018/annotation2048512/range2048512/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K256018/annotation2048512/range2048512/1
type: Range
persistent identity: range2048512
display id: range2048512
version: 1
direction: direction/forward
top level: BBa_K256018
start: 1039
end: 1118
orientation: inline

BBa_J23119
type: SequenceAnnotation
persistent identity: annotation2048508
display id: annotation2048508
version: 1
top level: BBa_K256018
location: https://synbiohub.org/public/igem/BBa_K256018/annotation2048508/range2048508/1
component: BBa_J23119

https://synbiohub.org/public/igem/BBa_K256018/annotation2048508/range2048508/1
type: Range
persistent identity: range2048508
display id: range2048508
version: 1
direction: direction/reverse
top level: BBa_K256018
start: 1
end: 35
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2048514
display id: annotation2048514
version: 1
top level: BBa_K256018
location: https://synbiohub.org/public/igem/BBa_K256018/annotation2048514/range2048514/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K256018/annotation2048514/range2048514/1
type: Range
persistent identity: range2048514
display id: range2048514
version: 1
direction: direction/forward
top level: BBa_K256018
start: 1127
end: 1167
orientation: inline

BBa_K256008
type: SequenceAnnotation
persistent identity: annotation2048511
display id: annotation2048511
version: 1
top level: BBa_K256018
location: https://synbiohub.org/public/igem/BBa_K256018/annotation2048511/range2048511/1
component: BBa_K256008

https://synbiohub.org/public/igem/BBa_K256018/annotation2048511/range2048511/1
type: Range
persistent identity: range2048511
display id: range2048511
version: 1
direction: direction/reverse
top level: BBa_K256018
start: 62
end: 1030
orientation: inline

R37
type: ComponentDefinition
persistent identity: BBa K780002
display id: BBa_K780002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Strong RBS for Bacillus Subtilis
creator: Krzysztof Szczepaniak
created: 2012-09-25T11:00:00Z
modified: 2015-05-08T01:13:18Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1034_
m user id: 0
owner id: 10113
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Strong RBS for Bacillus Subtilis
mutable notes: -
mutable provenance: 'host-takover module' from B.subtilis Bacteriophage SPO1
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_K780002_sequence/1

BBa_K1585115
type: ComponentDefinition
persistent identity: BBa K1585115
display id: BBa_K1585115
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Anderson Promoter with lacI binding site
creator: Tobias Schwanemann
created: 2015-08-31T11:00:00Z
modified: 2015-09-01T02:13:08Z
discontinued: false
dominant: false
experience: experience/Fails
group u list: _2002_
m user id: 24474
owner id: 24474
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Promoters of the Anderson Promoter Collection with lacI binding site derived from [http://parts.igem.org/Part:BBa_R0010 R_0010] to make them [http://openwetware.org/wiki/IPTG IPTG] iducible.
mutable notes: We choosed lacI-binding-site of part BBa_R0010 because we assumed older parts would be more reliable. The part is shipped in backbone [http://parts.igem.org/Part:BBa_J61002 J61002] because the functionality is directly visible by adding IPTG. RFP ([http://parts.igem.org/Part:BBa_E1010 E1010]) should get expressed then.
mutable provenance: Assambly of [http://parts.igem.org/Part:BBa_J23110 J23110] and lacI-binding-site of [http://parts.igem.org/Part:BBa_R0010 R_0010] The "lacI binding site" of Part [http://parts.igem.org/Part:BBa_R0010 R_0010] differs from lacO from Part [http://parts.igem.org/Part:BBa_J33207 BBa_J33207].
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
component: BBa_J23115
component: BBa_K1585999
sequence annotation: BBa_J23115
sequence annotation: BBa_K1585999
sequence: https://synbiohub.org/public/igem/BBa_K1585115_sequence/1

BBa_J23115
type: Component
persistent identity: component2441705
display id: component2441705
version: 1
top level: BBa_K1585115
access: public
definition: BBa_J23115

BBa_K1585999
type: Component
persistent identity: component2441707
display id: component2441707
version: 1
top level: BBa_K1585115
access: public
definition: https://synbiohub.org/public/igem/BBa_K1585999/1

BBa_J23115
type: SequenceAnnotation
persistent identity: annotation2441705
display id: annotation2441705
version: 1
top level: BBa_K1585115
location: https://synbiohub.org/public/igem/BBa_K1585115/annotation2441705/range2441705/1
component: BBa_J23115

https://synbiohub.org/public/igem/BBa_K1585115/annotation2441705/range2441705/1
type: Range
persistent identity: range2441705
display id: range2441705
version: 1
direction: direction/reverse
top level: BBa_K1585115
start: 1
end: 35
orientation: inline

BBa_K1585999
type: SequenceAnnotation
persistent identity: annotation2441707
display id: annotation2441707
version: 1
top level: BBa_K1585115
location: https://synbiohub.org/public/igem/BBa_K1585115/annotation2441707/range2441707/1
component: BBa_K1585999

https://synbiohub.org/public/igem/BBa_K1585115/annotation2441707/range2441707/1
type: Range
persistent identity: range2441707
display id: range2441707
version: 1
direction: direction/reverse
top level: BBa_K1585115
start: 44
end: 78
orientation: inline

BBa_M13505
type: ComponentDefinition
persistent identity: BBa M13505
display id: BBa_M13505
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: M13KO7 gene V RBS
creator: Natalie Kuldell
created: 2006-12-14T12:00:00Z
modified: 2015-05-08T01:13:56Z
discontinued: false
dominant: false
experience: experience/None
group u list: _45_
m user id: 0
owner id: 314
owning group id: 1
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This RBS is part of the bacteriophage M13 genome, initiating translation of the gene V message (BBa_M13005). The part aligns with base pairs 827-842 in M13K07 genome. It was identified as the RBS for the gene V message by Wezenbeek et al in Gene (1980) 11: 129-148
mutable notes: none
mutable provenance: New England Biolabs M13K07. Sequenced in 2002 by L. Panganaban
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_M13505_sequence/1

BBa_B0033
type: ComponentDefinition
persistent identity: BBa B0033
display id: BBa_B0033
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS.4 (weaker) -- derivative of BBa_0030
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _41_44_48_46_1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Weaker RBS based on Ron Weiss thesis. Strengths relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>.
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("RBS-3" in figure 4-14 of thesis). No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: BBa_B0033
sequence annotation: RBS-4\Weaker
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_B0033_sequence/1

BBa_B0033
type: SequenceAnnotation
persistent identity: annotation7028
display id: annotation7028
version: 1
top level: BBa_B0033
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_B0033/annotation7028/range7028/1

https://synbiohub.org/public/igem/BBa_B0033/annotation7028/range7028/1
type: Range
persistent identity: range7028
display id: range7028
version: 1
direction: direction/forward
top level: BBa_B0033
start: 1
end: 11
orientation: inline

RBS-4\Weaker
type: SequenceAnnotation
persistent identity: annotation1713
display id: annotation1713
version: 1
top level: BBa_B0033
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_B0033/annotation1713/range1713/1

https://synbiohub.org/public/igem/BBa_B0033/annotation1713/range1713/1
type: Range
persistent identity: range1713
display id: range1713
version: 1
direction: direction/forward
top level: BBa_B0033
start: 1
end: 11
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1714
display id: annotation1714
version: 1
top level: BBa_B0033
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_B0033/annotation1714/range1714/1

https://synbiohub.org/public/igem/BBa_B0033/annotation1714/range1714/1
type: Range
persistent identity: range1714
display id: range1714
version: 1
direction: direction/forward
top level: BBa_B0033
start: 7
end: 10
orientation: inline

RBS
type: ComponentDefinition
persistent identity: BBa J29048
display id: BBa_J29048
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS
creator: Maiko Furubayashi
created: 2006-10-19T11:00:00Z
modified: 2015-08-31T04:08:45Z
discontinued: false
dominant: false
experience: experience/None
group u list: _59_
m user id: 0
owner id: 846
owning group id: 59
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: strong rbs
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J29048_sequence/1

BBa_J23115
type: ComponentDefinition
persistent identity: BBa J23115
display id: BBa_J23115
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: constitutive promoter family member
creator: John Anderson
created: 2006-08-16T11:00:00Z
modified: 2015-08-31T04:08:40Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: true
mutable description: Later
mutable notes: N/A
mutable provenance: Later
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J23115_sequence/1

BBa_J64011
type: ComponentDefinition
persistent identity: BBa J64011
display id: BBa_J64011
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: invB RBS
creator: Dan Widmaier
created: 2008-01-15T12:00:00Z
modified: 2015-08-31T01:56:26Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _98_
m user id: 0
owner id: 2407
owning group id: 98
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: The natural RBS that drives invB
mutable notes: The expression level of this RBS has not been quantified.
mutable provenance: Salmonella LT2 Genomic DNA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: invB RBS
sequence: https://synbiohub.org/public/igem/BBa_J64011_sequence/1

invB RBS
type: SequenceAnnotation
persistent identity: annotation1959604
display id: annotation1959604
version: 1
top level: BBa_J64011
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_J64011/annotation1959604/range1959604/1

https://synbiohub.org/public/igem/BBa_J64011/annotation1959604/range1959604/1
type: Range
persistent identity: range1959604
display id: range1959604
version: 1
direction: direction/forward
top level: BBa_J64011
start: 1
end: 19
orientation: inline

BBa_I742150
type: ComponentDefinition
persistent identity: BBa I742150
display id: BBa_I742150
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: crtE native rbs
creator: Chris French
created: 2007-10-23T11:00:00Z
modified: 2015-08-31T04:08:03Z
discontinued: false
dominant: false
experience: experience/None
group u list: _123_
m user id: 0
owner id: 837
owning group id: 163
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is the native ribosome binding site of crtE (geranylgeranyl pyrophosphate synthase) from Pantoea ananatis (formerly Erwinia uredovora) DSMZ 30080 (ATCC 19321) Accession: D90087.
mutable notes: This is a virtual part to be added, blunt ended, to the coding sequence.
mutable provenance: Pantoea ananatis (formerly Erwinia uredovora) DSMZ 30080 (ATCC 19321) Accession: D90087.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_I742150_sequence/1

BBa_M13502
type: ComponentDefinition
persistent identity: BBa M13502
display id: BBa_M13502
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: M13KO7 gene II RBS
creator: Natalie Kuldell
created: 2006-12-14T12:00:00Z
modified: 2015-05-08T01:13:56Z
discontinued: false
dominant: false
experience: experience/None
group u list: _45_
m user id: 0
owner id: 314
owning group id: 1
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This RBS is part of the bacteriophage M13 genome, initiating translation of the gene II message (BBa_M13002). The part aligns with base pairs 8252-8267 in M13K07 genome. It was identified as the RBS for gene II message by Wezenbeek et al in Gene (1980) 11: 129-148
mutable notes: none
mutable provenance: New England Biolabs M13K07. Sequenced in 2002 by L. Panganaban
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_M13502_sequence/1

BBa_J23151
type: ComponentDefinition
persistent identity: BBa J23151
display id: BBa_J23151
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: 1bp mutant from J23114
creator: Jason Kelly
created: 2008-02-11T12:00:00Z
modified: 2015-08-31T04:08:41Z
discontinued: false
dominant: false
group u list: _11_
m user id: 0
owner id: 2
owning group id: 84
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: .
mutable notes: .
mutable provenance: .
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J23151_sequence/1

BBa_J64015
type: ComponentDefinition
persistent identity: BBa J64015
display id: BBa_J64015
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: sopA RBS
creator: Dan Widmaier
created: 2008-01-16T12:00:00Z
modified: 2015-08-31T01:56:26Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _98_
m user id: 0
owner id: 2407
owning group id: 98
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is the natural RBS associated with the sopA effector protein in the Salmonella SPI-1 system.
mutable notes: This part was not designed with compliance to biobrick standard assembly in mind. This RBS hasn't been explicitly tested in an expression assay such as GFP fluorescence or B-Gal expression.
mutable provenance: Salmonella LT2 Genomic DNA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: sopA RBS
sequence: https://synbiohub.org/public/igem/BBa_J64015_sequence/1

sopA RBS
type: SequenceAnnotation
persistent identity: annotation1959614
display id: annotation1959614
version: 1
top level: BBa_J64015
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_J64015/annotation1959614/range1959614/1

https://synbiohub.org/public/igem/BBa_J64015/annotation1959614/range1959614/1
type: Range
persistent identity: range1959614
display id: range1959614
version: 1
direction: direction/forward
top level: BBa_J64015
start: 1
end: 16
orientation: inline

BBa_J23108
type: ComponentDefinition
persistent identity: BBa J23108
display id: BBa_J23108
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: constitutive promoter family member
creator: John Anderson
created: 2006-08-16T11:00:00Z
modified: 2015-08-31T04:08:40Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: true
mutable description: Later
mutable notes: N/A
mutable provenance: Later
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J23108_sequence/1

BBa_J59001
type: ComponentDefinition
persistent identity: BBa J59001
display id: BBa_J59001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: tuba
creator: Fatos tuba
created: 2006-08-28T11:00:00Z
modified: 2015-08-31T02:02:59Z
discontinued: false
dominant: false
experience: experience/None
group u list: _93_
m user id: 0
owner id: 1162
owning group id: 93
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: none
mutable notes: none
mutable provenance: none
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: tuba
sequence: https://synbiohub.org/public/igem/BBa_J59001_sequence/1

tuba
type: SequenceAnnotation
persistent identity: annotation1898348
display id: annotation1898348
version: 1
top level: BBa_J59001
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_J59001/annotation1898348/range1898348/1

https://synbiohub.org/public/igem/BBa_J59001/annotation1898348/range1898348/1
type: Range
persistent identity: range1898348
display id: range1898348
version: 1
direction: direction/reverse
top level: BBa_J59001
start: 1
end: 25
orientation: inline

creD RBS
type: ComponentDefinition
persistent identity: BBa J64609
display id: BBa_J64609
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: creD RBS
creator: Ryan Ritterson
created: 2007-03-19T12:00:00Z
modified: 2015-05-08T01:08:17Z
discontinued: false
dominant: false
experience: experience/None
group u list: _98_
m user id: 0
owner id: 1422
owning group id: 98
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: The ribosomal binding site of the creD gene in the E Coli creD operon.
mutable notes: natural sequence, no design.
mutable provenance: E Coli Strain K12
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J64609_sequence/1

BBa_J61142
type: ComponentDefinition
persistent identity: BBa J61142
display id: BBa_J61142
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: {rbs1} Library Member in BBb
creator: John Anderson
created: 2007-09-03T11:00:00Z
modified: 2015-08-31T01:56:25Z
discontinued: false
dominant: false
experience: experience/None
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: {{BBbRBS1Family}}
mutable notes: {{BerkiGEM2007-Biobrick2.0Intro}}
mutable provenance: Synthetic
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J61142_sequence/1

BBa_M13503
type: ComponentDefinition
persistent identity: BBa M13503
display id: BBa_M13503
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: M13K07 gene III RBS
creator: Natalie Kuldell
created: 2006-12-14T12:00:00Z
modified: 2015-05-08T01:13:56Z
discontinued: false
dominant: false
experience: experience/None
group u list: _45_
m user id: 0
owner id: 314
owning group id: 1
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This RBS is part of the bacteriophage M13 genome, initiating translation of the gene III message (BBa_M13003). The part aligns with base pairs 1563-1578 in M13K07 genome. It was identified as the RBS for gene III message by Wezenbeek et al in Gene (1980) 11: 129-148 New England Biolabs M13K07. Sequenced in 2002 by L. Panganaban
mutable notes: none
mutable provenance: New England Biolabs M13K07. Sequenced in 2002 by L. Panganaban
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_M13503_sequence/1

BBa_B0031
type: ComponentDefinition
persistent identity: BBa B0031
display id: BBa_B0031
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS.2 (weak) -- derivative of BBa_0030
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _41_44_48_46_1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Medium RBS based on Ron Weiss thesis. Strength considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("RBS-1" in figure 4-14 of thesis). No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Cho
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0031_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23316
display id: annotation23316
version: 1
top level: BBa_B0031
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_B0031/annotation23316/range23316/1

https://synbiohub.org/public/igem/BBa_B0031/annotation23316/range23316/1
type: Range
persistent identity: range23316
display id: range23316
version: 1
direction: direction/forward
top level: BBa_B0031
start: 7
end: 10
orientation: inline

BBa_K1585104
type: ComponentDefinition
persistent identity: BBa K1585104
display id: BBa_K1585104
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Anderson Promoter with lacI binding site
creator: Tobias Schwanemann
created: 2015-08-31T11:00:00Z
modified: 2015-09-01T03:26:03Z
discontinued: false
dominant: false
experience: experience/Fails
group u list: _2002_
m user id: 24474
owner id: 24474
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Promoters of the Anderson Promoter Collection with lacI binding site derived from [http://parts.igem.org/Part:BBa_R0010 R_0010] to make them [http://openwetware.org/wiki/IPTG IPTG] iducible.
mutable notes: We choosed lacI-binding-site of part BBa_R0010 because we assumed older parts would be more reliable. The part is shipped in backbone [http://parts.igem.org/Part:BBa_J61002 J61002] because the functionality is directly visible by adding IPTG. RFP ([http://parts.igem.org/Part:BBa_E1010 E1010]) should get expressed then.
mutable provenance: Assambly of [http://parts.igem.org/Part:BBa_J23104 J23104] and lacI-binding-site of [http://parts.igem.org/Part:BBa_R0010 R_0010] The "lacI binding site" of Part [http://parts.igem.org/Part:BBa_R0010 R_0010] differs from lacO from Part [http://parts.igem.org/Part:BBa_J33207 BBa_J33207].
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
component: BBa_J23104
component: BBa_K1585999
sequence annotation: BBa_K1585999
sequence annotation: BBa_J23104
sequence: https://synbiohub.org/public/igem/BBa_K1585104_sequence/1

BBa_J23104
type: Component
persistent identity: component2441420
display id: component2441420
version: 1
top level: BBa_K1585104
access: public
definition: BBa_J23104

BBa_K1585999
type: Component
persistent identity: component2441422
display id: component2441422
version: 1
top level: BBa_K1585104
access: public
definition: https://synbiohub.org/public/igem/BBa_K1585999/1

BBa_K1585999
type: SequenceAnnotation
persistent identity: annotation2441422
display id: annotation2441422
version: 1
top level: BBa_K1585104
location: https://synbiohub.org/public/igem/BBa_K1585104/annotation2441422/range2441422/1
component: BBa_K1585999

https://synbiohub.org/public/igem/BBa_K1585104/annotation2441422/range2441422/1
type: Range
persistent identity: range2441422
display id: range2441422
version: 1
direction: direction/reverse
top level: BBa_K1585104
start: 44
end: 78
orientation: inline

BBa_J23104
type: SequenceAnnotation
persistent identity: annotation2441420
display id: annotation2441420
version: 1
top level: BBa_K1585104
location: https://synbiohub.org/public/igem/BBa_K1585104/annotation2441420/range2441420/1
component: BBa_J23104

https://synbiohub.org/public/igem/BBa_K1585104/annotation2441420/range2441420/1
type: Range
persistent identity: range2441420
display id: range2441420
version: 1
direction: direction/reverse
top level: BBa_K1585104
start: 1
end: 35
orientation: inline

BBa_B0072
type: ComponentDefinition
persistent identity: BBa B0072
display id: BBa_B0072
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: orthogonal RBS
creator: Bartholomew Canton
created: 2006-05-01T11:00:00Z
modified: 2015-08-31T04:07:21Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_6_
m user id: 0
owner id: 135
owning group id: 6
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_B0072_sequence/1

BBa_K137087
type: ComponentDefinition
persistent identity: BBa K137087
display id: BBa_K137087
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: optimized (TA) repeat constitutive promoter with 17 bp between -10 and -35 elements
creator: Allen Lin
created: 2008-08-20T11:00:00Z
modified: 2015-05-08T01:10:10Z
discontinued: false
dominant: false
experience: experience/None
group u list: _187_
m user id: 0
owner id: 3112
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: optimized (TA) repeat constitutive promoter with 17 bp between -10 and -35 elements
mutable notes: None
mutable provenance: PCR
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K137087_sequence/1

BBa_K150005
type: ComponentDefinition
persistent identity: BBa K150005
display id: BBa_K150005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: ribosome binding site of pTrc99a
creator: Pascal Kraemer
created: 2008-10-20T11:00:00Z
modified: 2015-05-08T01:10:46Z
discontinued: false
dominant: false
experience: experience/None
group u list: _234_
m user id: 0
owner id: 3299
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: ribosome binding site of pTrc99a
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: BBa_K150005
sequence: https://synbiohub.org/public/igem/BBa_K150005_sequence/1

BBa_K150005
type: SequenceAnnotation
persistent identity: annotation1983190
display id: annotation1983190
version: 1
top level: BBa_K150005
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K150005/annotation1983190/range1983190/1

https://synbiohub.org/public/igem/BBa_K150005/annotation1983190/range1983190/1
type: Range
persistent identity: range1983190
display id: range1983190
version: 1
direction: direction/forward
top level: BBa_K150005
start: 1
end: 13
orientation: inline

BBa_J61110
type: ComponentDefinition
persistent identity: BBa J61110
display id: BBa_J61110
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ribosome Binding Site Family Member
creator: John Anderson
created: 2007-04-23T11:00:00Z
modified: 2015-08-31T02:03:00Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable description: {{JCA_Arkin_RBSFamily}}
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J61110_sequence/1

BBa_K082000
type: ComponentDefinition
persistent identity: BBa K082000
display id: BBa_K082000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: reconstruct strong RBS
creator: Yanting Xue , Binjie Xu
created: 2008-10-27T12:00:00Z
modified: 2015-05-08T01:08:35Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _228_
m user id: 0
owner id: 1560
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Strong RBS based on Ron Weiss thesis. Strength is considered relative to BBa_B0031, BBa_B0032, BBa_B0033 and BBa_B0034.
mutable notes: The
mutable provenance: The
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: BBa_K082000
sequence: https://synbiohub.org/public/igem/BBa_K082000_sequence/1

BBa_K082000
type: SequenceAnnotation
persistent identity: annotation1992768
display id: annotation1992768
version: 1
top level: BBa_K082000
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K082000/annotation1992768/range1992768/1

https://synbiohub.org/public/igem/BBa_K082000/annotation1992768/range1992768/1
type: Range
persistent identity: range1992768
display id: range1992768
version: 1
direction: direction/reverse
top level: BBa_K082000
start: 1
end: 17
orientation: inline

BBa_K777004
type: ComponentDefinition
persistent identity: BBa K777004
display id: BBa_K777004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Tar receptor under the control of constitutive promoter J23106
creator: Bianca Genenncher
created: 2012-09-20T11:00:00Z
modified: 2015-05-08T01:13:16Z
discontinued: false
dominant: false
group u list: _1029_
m user id: 0
owner id: 11946
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: The transmembrane chemoreceptor Tar of E. coli mediates chemotaxis towards aspartate and exists as a functional homodimer. Here we used a set of 8 constitutive [http://partsregistry.org/Part:BBa_J23106 Anderson promoters] from the 2006 Berkeley group to test how different levels of constitutive ''Tar'' expression affect the chemotaxis of ''E. coli''.
mutable notes: * Genomic sequence was amplified using the following primers. ** Primers PCR: Base pairs in caps represent the respective restriction sites followed by either prefix or suffix. Primers were provided by SIGMA. *** Fwd: Tar for + prefix: 5?? actGAATTCgcggccgctTCTAGAtgattaaccgtatccgcgtagtc 3?? *** Rev: Tar rev + suffix: 5?? tcCTGCAGcggccgctACTAGTcaaaatgtttcccagtttggatc 3?? * One XbaI site was found at position 420. The restriction site was changed from TCTAGt to CCTAGt via QuikChange PCR. ** Primers QuikChange (QC): Primers were provided by SIGMA. *** Fwd: TarQC for: 5?? ACTGATTGATTAcCTAGATTATGGCAATACTGGAG 3?? *** Rev: TarQC rev: 5?? TGCCATAATCTAGgTAATCAATCAGTTCAGTTAAC 3?? (the uncapitalized letter induces the mutation for removal of the XbaI site)
mutable provenance: *The part was amplified from genomic DNA of ''E. coli'' str. K-12 substr. DH10B, complete genome (CP000948.1). *J23106 information was taken from the partsregistry and physical DNA from the 2012 distribution kit.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K777000
component: BBa_J23106
sequence annotation: BBa_K777000
sequence annotation: BBa_J23106
sequence: https://synbiohub.org/public/igem/BBa_K777004_sequence/1

BBa_K777000
type: Component
persistent identity: component2189302
display id: component2189302
version: 1
top level: BBa_K777004
access: public
definition: BBa_K777000

BBa_J23106
type: Component
persistent identity: component2189298
display id: component2189298
version: 1
top level: BBa_K777004
access: public
definition: BBa_J23106

BBa_K777000
type: SequenceAnnotation
persistent identity: annotation2189302
display id: annotation2189302
version: 1
top level: BBa_K777004
location: https://synbiohub.org/public/igem/BBa_K777004/annotation2189302/range2189302/1
component: BBa_K777000

https://synbiohub.org/public/igem/BBa_K777004/annotation2189302/range2189302/1
type: Range
persistent identity: range2189302
display id: range2189302
version: 1
direction: direction/reverse
top level: BBa_K777004
start: 42
end: 1703
orientation: inline

BBa_J23106
type: SequenceAnnotation
persistent identity: annotation2189298
display id: annotation2189298
version: 1
top level: BBa_K777004
location: https://synbiohub.org/public/igem/BBa_K777004/annotation2189298/range2189298/1
component: BBa_J23106

https://synbiohub.org/public/igem/BBa_K777004/annotation2189298/range2189298/1
type: Range
persistent identity: range2189298
display id: range2189298
version: 1
direction: direction/reverse
top level: BBa_K777004
start: 1
end: 35
orientation: inline

BBa_J23103
type: ComponentDefinition
persistent identity: BBa J23103
display id: BBa_J23103
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: constitutive promoter family member
creator: John Anderson
created: 2006-08-03T11:00:00Z
modified: 2015-08-31T04:08:40Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: replace later
mutable notes: N/A
mutable provenance: isolated from library of promoters
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J23103_sequence/1

BBa_J34803
type: ComponentDefinition
persistent identity: BBa J34803
display id: BBa_J34803
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: ribosome binding site
creator: Alexandra Choutko
created: 2006-10-25T11:00:00Z
modified: 2015-08-31T04:08:46Z
discontinued: false
dominant: false
experience: experience/None
group u list: _62_
m user id: 0
owner id: 1085
owning group id: 62
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: a
mutable notes: a
mutable provenance: a
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J34803_sequence/1

BBa_K137085
type: ComponentDefinition
persistent identity: BBa K137085
display id: BBa_K137085
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: optimized (TA) repeat constitutive promoter with 13 bp between -10 and -35 elements
creator: Allen Lin
created: 2008-08-20T11:00:00Z
modified: 2015-05-08T01:10:10Z
discontinued: false
dominant: false
experience: experience/None
group u list: _187_
m user id: 0
owner id: 3112
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: optimized (TA) repeat constitutive promoter with 13 bp between -10 and -35 elements
mutable notes: None
mutable provenance: PCR
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K137085_sequence/1

BBa_K777000
type: ComponentDefinition
persistent identity: BBa K777000
display id: BBa_K777000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Tar receptor
creator: Bianca Genenncher
created: 2012-09-10T11:00:00Z
modified: 2015-05-08T01:13:16Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1029_
m user id: 0
owner id: 11946
owning group id: 9
sample status: In stock
sample status: It's complicated
status: status/Available
status: status/Planning
bookmark: false
mutable description: The transmembrane receptor Tar of E. coli mediates chemotaxis towards aspartate.
mutable notes: One XbaI site was found at position 420. The restriction site was changed from TCTAGt to CCTAGt via QuikChange PCR.
mutable provenance: The part was amplified from genomic DNA of E. coli str. K-12 substr. DH10B chromosome (NC_010473.1).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Tar coding region
sequence annotation: ATG
sequence annotation: TGA
sequence: https://synbiohub.org/public/igem/BBa_K777000_sequence/1

Tar coding region
type: SequenceAnnotation
persistent identity: annotation2182660
display id: annotation2182660
version: 1
top level: BBa_K777000
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K777000/annotation2182660/range2182660/1

https://synbiohub.org/public/igem/BBa_K777000/annotation2182660/range2182660/1
type: Range
persistent identity: range2182660
display id: range2182660
version: 1
direction: direction/forward
top level: BBa_K777000
start: 1
end: 1662
orientation: inline

ATG
type: SequenceAnnotation
persistent identity: annotation2182659
display id: annotation2182659
version: 1
top level: BBa_K777000
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K777000/annotation2182659/range2182659/1

https://synbiohub.org/public/igem/BBa_K777000/annotation2182659/range2182659/1
type: Range
persistent identity: range2182659
display id: range2182659
version: 1
direction: direction/forward
top level: BBa_K777000
start: 1
end: 3
orientation: inline

TGA
type: SequenceAnnotation
persistent identity: annotation2182661
display id: annotation2182661
version: 1
top level: BBa_K777000
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K777000/annotation2182661/range2182661/1

https://synbiohub.org/public/igem/BBa_K777000/annotation2182661/range2182661/1
type: Range
persistent identity: range2182661
display id: range2182661
version: 1
direction: direction/forward
top level: BBa_K777000
start: 1660
end: 1662
orientation: inline

BBa_B0029
type: ComponentDefinition
persistent identity: BBa B0029
display id: BBa_B0029
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: 1 bp deletion from B0031
creator: Jason Kelly
created: 2008-02-07T12:00:00Z
modified: 2015-08-31T04:07:20Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_
m user id: 0
owner id: 2
owning group id: 84
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: .
mutable notes: .
mutable provenance: .
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_B0029_sequence/1

BBa_J34810
type: ComponentDefinition
persistent identity: BBa J34810
display id: BBa_J34810
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: ribosome binding site
creator: Alexandra Choutko
created: 2006-10-25T11:00:00Z
modified: 2015-08-31T04:08:47Z
discontinued: false
dominant: false
experience: experience/None
group u list: _62_
m user id: 0
owner id: 1085
owning group id: 62
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: a
mutable notes: a
mutable provenance: a
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J34810_sequence/1

BBa_J61103
type: ComponentDefinition
persistent identity: BBa J61103
display id: BBa_J61103
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ribosome Binding Site Family Member
creator: John Anderson
created: 2007-04-23T11:00:00Z
modified: 2015-08-31T02:03:00Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable description: {{JCA_Arkin_RBSFamily}}
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J61103_sequence/1

BBa_K777002
type: ComponentDefinition
persistent identity: BBa K777002
display id: BBa_K777002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Tar receptor under the control of constitutive promoter J23104
creator: Bianca Genenncher
created: 2012-09-20T11:00:00Z
modified: 2015-05-08T01:13:16Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1029_
m user id: 0
owner id: 11946
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: The transmembrane chemoreceptor Tar of E. coli mediates chemotaxis towards aspartate and exists as a functional homodimer. Here we used a set of 8 constitutive [http://partsregistry.org/Part:BBa_J23100 Anderson promoters] from the 2006 Berkeley group to test how different levels of constitutive ''Tar'' expression affect the chemotaxis of ''E. coli''.
mutable notes: * Genomic sequence was amplified using the following primers. ** Primers PCR: Base pairs in caps represent the respective restriction sites followed by either prefix or suffix. Primers were provided by SIGMA. *** Fwd: Tar for + prefix: 5?? actGAATTCgcggccgctTCTAGAtgattaaccgtatccgcgtagtc 3?? *** Rev: Tar rev + suffix: 5?? tcCTGCAGcggccgctACTAGTcaaaatgtttcccagtttggatc 3?? * One XbaI site was found at position 420. The restriction site was changed from TCTAGt to CCTAGt via QuikChange PCR. ** Primers QuikChange (QC): Primers were provided by SIGMA. *** Fwd: TarQC for: 5?? ACTGATTGATTAcCTAGATTATGGCAATACTGGAG 3?? *** Rev: TarQC rev: 5?? TGCCATAATCTAGgTAATCAATCAGTTCAGTTAAC 3?? (the uncapitalized letter induces the mutation for removal of the XbaI site)
mutable provenance: *The part was amplified from genomic DNA of ''E. coli'' str. K-12 substr. DH10B, complete genome (CP000948.1). *J23100 information was taken from the partsregistry and physical DNA from the 2012 distribution kit.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_J23104
component: BBa_K777000
sequence annotation: BBa_J23104
sequence annotation: BBa_K777000
sequence: https://synbiohub.org/public/igem/BBa_K777002_sequence/1

BBa_J23104
type: Component
persistent identity: component2189238
display id: component2189238
version: 1
top level: BBa_K777002
access: public
definition: BBa_J23104

BBa_K777000
type: Component
persistent identity: component2189242
display id: component2189242
version: 1
top level: BBa_K777002
access: public
definition: BBa_K777000

BBa_J23104
type: SequenceAnnotation
persistent identity: annotation2189238
display id: annotation2189238
version: 1
top level: BBa_K777002
location: https://synbiohub.org/public/igem/BBa_K777002/annotation2189238/range2189238/1
component: BBa_J23104

https://synbiohub.org/public/igem/BBa_K777002/annotation2189238/range2189238/1
type: Range
persistent identity: range2189238
display id: range2189238
version: 1
direction: direction/reverse
top level: BBa_K777002
start: 1
end: 35
orientation: inline

BBa_K777000
type: SequenceAnnotation
persistent identity: annotation2189242
display id: annotation2189242
version: 1
top level: BBa_K777002
location: https://synbiohub.org/public/igem/BBa_K777002/annotation2189242/range2189242/1
component: BBa_K777000

https://synbiohub.org/public/igem/BBa_K777002/annotation2189242/range2189242/1
type: Range
persistent identity: range2189242
display id: range2189242
version: 1
direction: direction/reverse
top level: BBa_K777002
start: 42
end: 1703
orientation: inline

UPcox1
type: ComponentDefinition
persistent identity: BBa K809003
display id: BBa_K809003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Up elements of cox1 gene
creator: Qiwu XU
created: 2012-09-19T11:00:00Z
modified: 2015-05-08T01:13:26Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1066_
m user id: 0
owner id: 11943
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: Up elements (including promoter and RBS) of cox2 gene from Saccharomyces cerevisiae mitochondrial DNA.
mutable notes: Designed by Qiwu XU, BioBrick restriction enzyme sites added.
mutable provenance: Synthesized from LifeTechonology
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: cox1 up elements
sequence: https://synbiohub.org/public/igem/BBa_K809003_sequence/1

cox1 up elements
type: SequenceAnnotation
persistent identity: annotation2188526
display id: annotation2188526
version: 1
top level: UPcox1
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K809003/annotation2188526/range2188526/1

https://synbiohub.org/public/igem/BBa_K809003/annotation2188526/range2188526/1
type: Range
persistent identity: range2188526
display id: range2188526
version: 1
direction: direction/forward
top level: UPcox1
start: 497
end: 497
orientation: inline

cox1 up elements
type: SequenceAnnotation
persistent identity: annotation2188525
display id: annotation2188525
version: 1
top level: UPcox1
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K809003/annotation2188525/range2188525/1

https://synbiohub.org/public/igem/BBa_K809003/annotation2188525/range2188525/1
type: Range
persistent identity: range2188525
display id: range2188525
version: 1
direction: direction/forward
top level: UPcox1
start: 1
end: 1
orientation: inline

BBa_J23105
type: ComponentDefinition
persistent identity: BBa J23105
display id: BBa_J23105
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: constitutive promoter family member
creator: John Anderson
created: 2006-08-13T11:00:00Z
modified: 2015-08-31T04:08:40Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: true
mutable description: Later
mutable notes: N/A
mutable provenance: Isolated from library of promoters
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J23105_sequence/1

BBa_K113012
type: ComponentDefinition
persistent identity: BBa K113012
display id: BBa_K113012
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: weaken overlapping T7 promoter
creator: Wu Jingjing, Wang Jinyu
created: 2008-10-25T11:00:00Z
modified: 2015-05-08T01:09:20Z
discontinued: false
dominant: false
experience: experience/None
group u list: _254_
m user id: 0
owner id: 2711
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Two T7 promoters are reversely overlapping in two base pairs while a point mutation occurs at the 12th base pair, from A-T to T-A.
mutable notes: It is difficult to make this short DNA by PCR as both sides are T7 promoters, which are very similar. It is highly possible that they form loops before they can be synthesized by PCR. Thus we order two single stranded DNA and then anneal them together by touchdown PCR.
mutable provenance: The sequence of T7 promoter is TAATACGACTCACTATA (from 5' to 3').
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: T7 promoter
sequence: https://synbiohub.org/public/igem/BBa_K113012_sequence/1

T7 promoter
type: SequenceAnnotation
persistent identity: annotation1989400
display id: annotation1989400
version: 1
top level: BBa_K113012
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K113012/annotation1989400/range1989400/1

https://synbiohub.org/public/igem/BBa_K113012/annotation1989400/range1989400/1
type: Range
persistent identity: range1989400
display id: range1989400
version: 1
direction: direction/reverse
top level: BBa_K113012
start: 4
end: 21
orientation: inline

T7 promoter
type: SequenceAnnotation
persistent identity: annotation1989402
display id: annotation1989402
version: 1
top level: BBa_K113012
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K113012/annotation1989402/range1989402/1

https://synbiohub.org/public/igem/BBa_K113012/annotation1989402/range1989402/1
type: Range
persistent identity: range1989402
display id: range1989402
version: 1
direction: direction/forward
top level: BBa_K113012
start: 20
end: 37
orientation: inline

BBa_K112707
type: ComponentDefinition
persistent identity: BBa K112707
display id: BBa_K112707
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Pspv from Salmonella
creator: Bing Xia
created: 2008-10-20T11:00:00Z
modified: 2015-05-08T01:09:18Z
discontinued: false
dominant: false
experience: experience/None
group u list: _224_
m user id: 0
owner id: 2998
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: The spv promoter. ---- This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at: [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
mutable provenance: Construction of <Pspv> basic part K112707 PCR of dv017/dv016 on pBca1037 (499bp, EcoRI/BamHI/DpnI) Sub into pBca1256 (EcoRI/BamHI, 2472+499, L) Product is pBca1256-K112707 ----------------------------------------------- dv017 Forward biobricking of <Pspv> cgataGAATTCatgAGATCTaGATCCTGTGATGTTTGGCG dv016 Reverse biobricking of <Pspv> cgttaGGATCCGATAATGTtTGCAGGGGAATTATTTTG
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K112707_sequence/1

BBa_K1895003
type: ComponentDefinition
persistent identity: BBa K1895003
display id: BBa_K1895003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: htpG Promoter
creator: iGEM16_Newcastle
created: 2016-09-05T11:00:00Z
modified: 2016-09-06T04:50:25Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2361_
m user id: 29859
owner id: 29859
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This is the promoter for molecular chaperone htpG. It is active during the heat shock response.
mutable notes: [TODO]
mutable provenance: This is a correction to part [http://parts.igem.org/Part:BBa_J45504 BBa_J45504], also the htpG Heat Shock Promoter. The original part contains a fragment of the coding sequence for molecular chaperone htpG (base pairs >287) which can be confirmed by [http://blast.ncbi.nlm.nih.gov/ BLASTing the sequence]. This part contains only the first 287 bases of the original part which consist solely of the htpG promoter. You can see the difference between the two sequences in the image below.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K1895003_sequence/1

BBa_I723012
type: ComponentDefinition
persistent identity: BBa I723012
display id: BBa_I723012
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Estimated RBS for DntR
creator: Scott Ramsay
created: 2007-10-24T11:00:00Z
modified: 2015-08-31T04:07:53Z
discontinued: false
dominant: false
experience: experience/None
group u list: _126_
m user id: 0
owner id: 2140
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is the naturally-occurring RBS found in the regulatory region of the DntR gene.
mutable notes: '''''This does not physically exist as a basic part due to time constraints; it was cloned as part of composite part which incorporates all of the components necessary for expression of a reporter gene under the control of DntR'''''. For this reason, the ends of the supplied sequence may not accurately represent the boundaries of the functional unit.
mutable provenance: Cloned from a historical plasmid based on pQF52 carrying genomic sequences from ''Burkholderia cepacia''.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: RBS for DntR
sequence: https://synbiohub.org/public/igem/BBa_I723012_sequence/1

RBS for DntR
type: SequenceAnnotation
persistent identity: annotation1954958
display id: annotation1954958
version: 1
top level: BBa_I723012
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_I723012/annotation1954958/range1954958/1

https://synbiohub.org/public/igem/BBa_I723012/annotation1954958/range1954958/1
type: Range
persistent identity: range1954958
display id: range1954958
version: 1
direction: direction/forward
top level: BBa_I723012
start: 1
end: 24
orientation: inline

BBa_K823010
type: ComponentDefinition
persistent identity: BBa K823010
display id: BBa_K823010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Anderson promoter J23113
creator: Korinna Kraft
created: 2012-09-07T11:00:00Z
modified: 2015-07-07T11:47:51Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1081_
m user id: 4206
owner id: 12081
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Anderson promoter J23113 without RFP in backbone vector pSB1C3 to easily fuse the promoter with other reporters e.g. the lux operon.
mutable notes: no considerations
mutable provenance: Partsregistry
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -35 box
sequence annotation: J23113
sequence annotation: -10 box
sequence: https://synbiohub.org/public/igem/BBa_K823010_sequence/1

-35 box
type: SequenceAnnotation
persistent identity: annotation2182535
display id: annotation2182535
version: 1
top level: BBa_K823010
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K823010/annotation2182535/range2182535/1

https://synbiohub.org/public/igem/BBa_K823010/annotation2182535/range2182535/1
type: Range
persistent identity: range2182535
display id: range2182535
version: 1
direction: direction/forward
top level: BBa_K823010
start: 1
end: 6
orientation: inline

J23113
type: SequenceAnnotation
persistent identity: annotation2182534
display id: annotation2182534
version: 1
top level: BBa_K823010
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K823010/annotation2182534/range2182534/1

https://synbiohub.org/public/igem/BBa_K823010/annotation2182534/range2182534/1
type: Range
persistent identity: range2182534
display id: range2182534
version: 1
direction: direction/forward
top level: BBa_K823010
start: 1
end: 35
orientation: inline

-10 box
type: SequenceAnnotation
persistent identity: annotation2182536
display id: annotation2182536
version: 1
top level: BBa_K823010
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K823010/annotation2182536/range2182536/1

https://synbiohub.org/public/igem/BBa_K823010/annotation2182536/range2182536/1
type: Range
persistent identity: range2182536
display id: range2182536
version: 1
direction: direction/forward
top level: BBa_K823010
start: 24
end: 29
orientation: inline

BBa_Z0261
type: ComponentDefinition
persistent identity: BBa Z0261
display id: BBa_Z0261
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Strong T7.2 RBS
creator: Sriram Kosuri
created: 2006-05-15T11:00:00Z
modified: 2015-05-08T01:14:55Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_10_
m user id: 0
owner id: 64
owning group id: 10
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Sequence comes from the natural gene 5 RBS. Using gene 5 as a strong RBS is from initial results from experiments characterizing all natural T7 RBSs.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_Z0261_sequence/1

BBa_K823002
type: ComponentDefinition
persistent identity: BBa K823002
display id: BBa_K823002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PlepA
creator: Korinna Kraft
created: 2012-07-15T11:00:00Z
modified: 2015-05-08T01:13:29Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1081_
m user id: 0
owner id: 12081
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: PlepA is the promoter of the PlepA gene of Bacillus subtilis. It is a constitutive promoter and does not contain a ribosome binding site.
mutable notes: no considerations
mutable provenance: Bacillus subtilis
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K823002_sequence/1

BBa_K1585103
type: ComponentDefinition
persistent identity: BBa K1585103
display id: BBa_K1585103
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Anderson Promoter with lacI binding site
creator: Tobias Schwanemann
created: 2015-08-31T11:00:00Z
modified: 2015-09-01T03:08:18Z
discontinued: false
dominant: false
experience: experience/Fails
group u list: _2002_
m user id: 24474
owner id: 24474
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Promoters of the Anderson Promoter Collection with lacI binding site derived from [http://parts.igem.org/Part:BBa_R0010 R_0010] to make them [http://openwetware.org/wiki/IPTG IPTG] iducible.
mutable notes: We choosed lacI-binding-site of part BBa_R0010 because we assumed older parts would be more reliable. The part is shipped in backbone [http://parts.igem.org/Part:BBa_J61002 J61002] because the functionality is directly visible by adding IPTG. RFP ([http://parts.igem.org/Part:BBa_E1010 E1010]) should get expressed then.
mutable provenance: Assambly of [http://parts.igem.org/Part:BBa_J23103 J23103] and lacI-binding-site of [http://parts.igem.org/Part:BBa_R0010 R_0010] The "lacI binding site" of Part [http://parts.igem.org/Part:BBa_R0010 R_0010] differs from lacO from Part [http://parts.igem.org/Part:BBa_J33207 BBa_J33207].
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
component: BBa_K1585999
component: BBa_J23103
sequence annotation: BBa_J23103
sequence annotation: BBa_K1585999
sequence: https://synbiohub.org/public/igem/BBa_K1585103_sequence/1

BBa_K1585999
type: Component
persistent identity: component2441413
display id: component2441413
version: 1
top level: BBa_K1585103
access: public
definition: https://synbiohub.org/public/igem/BBa_K1585999/1

BBa_J23103
type: Component
persistent identity: component2441411
display id: component2441411
version: 1
top level: BBa_K1585103
access: public
definition: BBa_J23103

BBa_J23103
type: SequenceAnnotation
persistent identity: annotation2441411
display id: annotation2441411
version: 1
top level: BBa_K1585103
location: https://synbiohub.org/public/igem/BBa_K1585103/annotation2441411/range2441411/1
component: BBa_J23103

https://synbiohub.org/public/igem/BBa_K1585103/annotation2441411/range2441411/1
type: Range
persistent identity: range2441411
display id: range2441411
version: 1
direction: direction/reverse
top level: BBa_K1585103
start: 1
end: 35
orientation: inline

BBa_K1585999
type: SequenceAnnotation
persistent identity: annotation2441413
display id: annotation2441413
version: 1
top level: BBa_K1585103
location: https://synbiohub.org/public/igem/BBa_K1585103/annotation2441413/range2441413/1
component: BBa_K1585999

https://synbiohub.org/public/igem/BBa_K1585103/annotation2441413/range2441413/1
type: Range
persistent identity: range2441413
display id: range2441413
version: 1
direction: direction/reverse
top level: BBa_K1585103
start: 44
end: 78
orientation: inline

BBa_K1824896
type: ComponentDefinition
persistent identity: BBa K1824896
display id: BBa_K1824896
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: J23100 + RBS
creator: Xinhao Wang
created: 2015-09-10T11:00:00Z
modified: 2015-09-11T09:57:14Z
discontinued: false
dominant: false
group u list: _2250_
m user id: 26000
owner id: 26000
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Constitutive promoter J23100 plus RBS.
mutable notes: no special concern
mutable provenance: Parts J23100 through J23119 are a family of constitutive promoter parts isolated from a small combinatorial library. B0030 is a strong RBS based on Ron Weiss thesis
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1824889
component: BBa_K1824890
component: BBa_B0030
component: BBa_J23100
sequence annotation: BBa_K1824889
sequence annotation: BBa_K1824890
sequence annotation: BBa_J23100
sequence annotation: BBa_B0030
sequence: https://synbiohub.org/public/igem/BBa_K1824896_sequence/1

BBa_K1824889
type: Component
persistent identity: component2452341
display id: component2452341
version: 1
top level: BBa_K1824896
access: public
definition: https://synbiohub.org/public/igem/BBa_K1824889/1

BBa_K1824890
type: Component
persistent identity: component2452345
display id: component2452345
version: 1
top level: BBa_K1824896
access: public
definition: https://synbiohub.org/public/igem/BBa_K1824890/1

BBa_B0030
type: Component
persistent identity: component2452343
display id: component2452343
version: 1
top level: BBa_K1824896
access: public
definition: BBa_B0030

BBa_J23100
type: Component
persistent identity: component2452340
display id: component2452340
version: 1
top level: BBa_K1824896
access: public
definition: BBa_J23100

BBa_K1824889
type: SequenceAnnotation
persistent identity: annotation2452341
display id: annotation2452341
version: 1
top level: BBa_K1824896
location: https://synbiohub.org/public/igem/BBa_K1824896/annotation2452341/range2452341/1
component: BBa_K1824889

https://synbiohub.org/public/igem/BBa_K1824896/annotation2452341/range2452341/1
type: Range
persistent identity: range2452341
display id: range2452341
version: 1
direction: direction/reverse
top level: BBa_K1824896
start: 44
end: 51
orientation: inline

BBa_K1824890
type: SequenceAnnotation
persistent identity: annotation2452345
display id: annotation2452345
version: 1
top level: BBa_K1824896
location: https://synbiohub.org/public/igem/BBa_K1824896/annotation2452345/range2452345/1
component: BBa_K1824890

https://synbiohub.org/public/igem/BBa_K1824896/annotation2452345/range2452345/1
type: Range
persistent identity: range2452345
display id: range2452345
version: 1
direction: direction/reverse
top level: BBa_K1824896
start: 83
end: 88
orientation: inline

BBa_J23100
type: SequenceAnnotation
persistent identity: annotation2452340
display id: annotation2452340
version: 1
top level: BBa_K1824896
location: https://synbiohub.org/public/igem/BBa_K1824896/annotation2452340/range2452340/1
component: BBa_J23100

https://synbiohub.org/public/igem/BBa_K1824896/annotation2452340/range2452340/1
type: Range
persistent identity: range2452340
display id: range2452340
version: 1
direction: direction/reverse
top level: BBa_K1824896
start: 1
end: 35
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation2452343
display id: annotation2452343
version: 1
top level: BBa_K1824896
location: https://synbiohub.org/public/igem/BBa_K1824896/annotation2452343/range2452343/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_K1824896/annotation2452343/range2452343/1
type: Range
persistent identity: range2452343
display id: range2452343
version: 1
direction: direction/forward
top level: BBa_K1824896
start: 60
end: 74
orientation: inline

BBa_J64807
type: ComponentDefinition
persistent identity: BBa J64807
display id: BBa_J64807
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RocD RBS
creator: David Stanley
created: 2007-03-19T12:00:00Z
modified: 2015-05-08T01:08:17Z
discontinued: false
dominant: false
experience: experience/None
group u list: _98_
m user id: 0
owner id: 1423
owning group id: 98
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: RocD RBS
mutable notes: none
mutable provenance: none
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J64807_sequence/1

BBa_J61143
type: ComponentDefinition
persistent identity: BBa J61143
display id: BBa_J61143
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: {rbs1} Library Member in BBb
creator: John Anderson
created: 2007-09-03T11:00:00Z
modified: 2015-08-31T01:56:25Z
discontinued: false
dominant: false
experience: experience/None
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: {{BBbRBS1Family}}
mutable notes: {{BerkiGEM2007-Biobrick2.0Intro}}
mutable provenance: Synthetic
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J61143_sequence/1

BBa_K823003
type: ComponentDefinition
persistent identity: BBa K823003
display id: BBa_K823003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Pveg
creator: Korinna Kraft
created: 2012-07-15T11:00:00Z
modified: 2015-05-08T01:13:29Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1081_
m user id: 0
owner id: 12081
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Pveg is a strong, constitutive promoter of Bacillus subtilis
mutable notes: no considerations
mutable provenance: Bacillus subtilis
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: promoter
sequence: https://synbiohub.org/public/igem/BBa_K823003_sequence/1

promoter
type: SequenceAnnotation
persistent identity: annotation2190180
display id: annotation2190180
version: 1
top level: BBa_K823003
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K823003/annotation2190180/range2190180/1

https://synbiohub.org/public/igem/BBa_K823003/annotation2190180/range2190180/1
type: Range
persistent identity: range2190180
display id: range2190180
version: 1
direction: direction/forward
top level: BBa_K823003
start: 1
end: 237
orientation: inline

BBa_K382034
type: ComponentDefinition
persistent identity: BBa K382034
display id: BBa_K382034
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Act2pLacO (Actin promoter + 2x LacO)
creator: Mara C. Inniss
created: 2010-10-25T11:00:00Z
modified: 2015-06-11T01:09:19Z
discontinued: false
dominant: false
group u list: _488_
m user id: 4206
owner id: 7454
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: A constitutive plant actin promoter followed by two copies of the Lac operator. This allows control of promoter activity by LacI.
mutable notes: Conforms to biobrick standard 21
mutable provenance: synthesized by mr.gene
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K382034_sequence/1

BBa_J61111
type: ComponentDefinition
persistent identity: BBa J61111
display id: BBa_J61111
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ribosome Binding Site Family Member
creator: John Anderson
created: 2007-04-23T11:00:00Z
modified: 2015-08-31T02:03:00Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable description: {{JCA_Arkin_RBSFamily}}
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J61111_sequence/1

BBa_K1467104
type: ComponentDefinition
persistent identity: BBa K1467104
display id: BBa_K1467104
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: GoldenGate compatible MAS promoter
creator: Cara Deal
created: 2014-09-23T11:00:00Z
modified: 2015-05-29T01:22:30Z
discontinued: false
dominant: false
group u list: _1846_
m user id: 4206
owner id: 21550
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: MAS is a plant specific promoter which is constitutively expressed. This part is compatible with the GoldenGate MoClo Assembly Standard as it is free from BsaI and BpiI recognition sequences.
mutable notes: Domestication for GoldenGate by removal of BsaI and BpiI recognition sequences.
mutable provenance: Agrobacterium tumefaciens
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K1467104_sequence/1

BBa_J48104
type: ComponentDefinition
persistent identity: BBa J48104
display id: BBa_J48104
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: NikR promoter, a protein of the ribbon helix-helix family of trancription factors that repress expre
creator: Laina Magaya
created: 2006-10-30T12:00:00Z
modified: 2015-08-31T04:08:51Z
discontinued: false
dominant: false
experience: experience/None
group u list: _73_
m user id: 0
owner id: 969
owning group id: 73
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Nickel responsive transcriptional regulator
mutable notes: Cut with Pst I and Xba I
mutable provenance: Escherichia coli UTI89
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J48104_sequence/1

BBa_K1390019
type: ComponentDefinition
persistent identity: BBa K1390019
display id: BBa_K1390019
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Complete sMMO of Methylococcus capsulatus
creator: Torsten Meyer
created: 2014-09-03T11:00:00Z
modified: 2015-05-08T01:10:14Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1767_
m user id: 0
owner id: 17096
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: The part consists of the six subunits of methane monooxygenase (MMO). Gene order: mmoX, mmoY, mmoB, mmoZ, mmoD, mmoC The expression is under control of lac+pl. MMO converts methane to methanol.
mutable notes: Part is designed considering the RFC10 standard.
mutable provenance: Methylococcus capsulatus strain Bath
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K1390002
component: BBa_R0011
component: BBa_B0032
component: BBa_K1390006
component: BBa_B0015
component: BBa_K1390005
component: BBa_K1390001
component: BBa_K1390003
component: BBa_K1390004
sequence annotation: BBa_K1390002
sequence annotation: BBa_B0032
sequence annotation: BBa_K1390006
sequence annotation: BBa_R0011
sequence annotation: BBa_K1390001
sequence annotation: BBa_K1390005
sequence annotation: BBa_B0015
sequence annotation: BBa_K1390003
sequence annotation: BBa_K1390004
sequence: https://synbiohub.org/public/igem/BBa_K1390019_sequence/1

BBa_K1390002
type: Component
persistent identity: component2412377
display id: component2412377
version: 1
top level: BBa_K1390019
access: public
definition: https://synbiohub.org/public/igem/BBa_K1390002/1

BBa_R0011
type: Component
persistent identity: component2412349
display id: component2412349
version: 1
top level: BBa_K1390019
access: public
definition: https://synbiohub.org/public/igem/BBa_R0011/1

BBa_B0032
type: Component
persistent identity: component2412363
display id: component2412363
version: 1
top level: BBa_K1390019
access: public
definition: BBa_B0032

BBa_K1390006
type: Component
persistent identity: component2412369
display id: component2412369
version: 1
top level: BBa_K1390019
access: public
definition: https://synbiohub.org/public/igem/BBa_K1390006/1

BBa_B0032
type: Component
persistent identity: component2412375
display id: component2412375
version: 1
top level: BBa_K1390019
access: public
definition: BBa_B0032

BBa_B0032
type: Component
persistent identity: component2412359
display id: component2412359
version: 1
top level: BBa_K1390019
access: public
definition: BBa_B0032

BBa_B0032
type: Component
persistent identity: component2412355
display id: component2412355
version: 1
top level: BBa_K1390019
access: public
definition: BBa_B0032

BBa_B0015
type: Component
persistent identity: component2412384
display id: component2412384
version: 1
top level: BBa_K1390019
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_K1390005
type: Component
persistent identity: component2412361
display id: component2412361
version: 1
top level: BBa_K1390019
access: public
definition: https://synbiohub.org/public/igem/BBa_K1390005/1

BBa_K1390001
type: Component
persistent identity: component2412365
display id: component2412365
version: 1
top level: BBa_K1390019
access: public
definition: https://synbiohub.org/public/igem/BBa_K1390001/1

BBa_K1390003
type: Component
persistent identity: component2412373
display id: component2412373
version: 1
top level: BBa_K1390019
access: public
definition: https://synbiohub.org/public/igem/BBa_K1390003/1

BBa_K1390004
type: Component
persistent identity: component2412357
display id: component2412357
version: 1
top level: BBa_K1390019
access: public
definition: https://synbiohub.org/public/igem/BBa_K1390004/1

BBa_B0032
type: Component
persistent identity: component2412367
display id: component2412367
version: 1
top level: BBa_K1390019
access: public
definition: BBa_B0032

BBa_B0032
type: Component
persistent identity: component2412371
display id: component2412371
version: 1
top level: BBa_K1390019
access: public
definition: BBa_B0032

BBa_K1390002
type: SequenceAnnotation
persistent identity: annotation2412377
display id: annotation2412377
version: 1
top level: BBa_K1390019
location: https://synbiohub.org/public/igem/BBa_K1390019/annotation2412377/range2412377/1
component: BBa_K1390002

https://synbiohub.org/public/igem/BBa_K1390019/annotation2412377/range2412377/1
type: Range
persistent identity: range2412377
display id: range2412377
version: 1
direction: direction/reverse
top level: BBa_K1390019
start: 4220
end: 5266
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation2412359
display id: annotation2412359
version: 1
top level: BBa_K1390019
location: https://synbiohub.org/public/igem/BBa_K1390019/annotation2412359/range2412359/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_K1390019/annotation2412359/range2412359/1
type: Range
persistent identity: range2412359
display id: range2412359
version: 1
direction: direction/forward
top level: BBa_K1390019
start: 1675
end: 1687
orientation: inline

BBa_K1390006
type: SequenceAnnotation
persistent identity: annotation2412369
display id: annotation2412369
version: 1
top level: BBa_K1390019
location: https://synbiohub.org/public/igem/BBa_K1390019/annotation2412369/range2412369/1
component: BBa_K1390006

https://synbiohub.org/public/igem/BBa_K1390019/annotation2412369/range2412369/1
type: Range
persistent identity: range2412369
display id: range2412369
version: 1
direction: direction/reverse
top level: BBa_K1390019
start: 3344
end: 3853
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation2412355
display id: annotation2412355
version: 1
top level: BBa_K1390019
location: https://synbiohub.org/public/igem/BBa_K1390019/annotation2412355/range2412355/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_K1390019/annotation2412355/range2412355/1
type: Range
persistent identity: range2412355
display id: range2412355
version: 1
direction: direction/forward
top level: BBa_K1390019
start: 64
end: 76
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation2412363
display id: annotation2412363
version: 1
top level: BBa_K1390019
location: https://synbiohub.org/public/igem/BBa_K1390019/annotation2412363/range2412363/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_K1390019/annotation2412363/range2412363/1
type: Range
persistent identity: range2412363
display id: range2412363
version: 1
direction: direction/forward
top level: BBa_K1390019
start: 2872
end: 2884
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation2412371
display id: annotation2412371
version: 1
top level: BBa_K1390019
location: https://synbiohub.org/public/igem/BBa_K1390019/annotation2412371/range2412371/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_K1390019/annotation2412371/range2412371/1
type: Range
persistent identity: range2412371
display id: range2412371
version: 1
direction: direction/forward
top level: BBa_K1390019
start: 3862
end: 3874
orientation: inline

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation2412349
display id: annotation2412349
version: 1
top level: BBa_K1390019
location: https://synbiohub.org/public/igem/BBa_K1390019/annotation2412349/range2412349/1
component: BBa_R0011

https://synbiohub.org/public/igem/BBa_K1390019/annotation2412349/range2412349/1
type: Range
persistent identity: range2412349
display id: range2412349
version: 1
direction: direction/forward
top level: BBa_K1390019
start: 1
end: 54
orientation: inline

BBa_K1390001
type: SequenceAnnotation
persistent identity: annotation2412365
display id: annotation2412365
version: 1
top level: BBa_K1390019
location: https://synbiohub.org/public/igem/BBa_K1390019/annotation2412365/range2412365/1
component: BBa_K1390001

https://synbiohub.org/public/igem/BBa_K1390019/annotation2412365/range2412365/1
type: Range
persistent identity: range2412365
display id: range2412365
version: 1
direction: direction/reverse
top level: BBa_K1390019
start: 2891
end: 3316
orientation: inline

BBa_K1390005
type: SequenceAnnotation
persistent identity: annotation2412361
display id: annotation2412361
version: 1
top level: BBa_K1390019
location: https://synbiohub.org/public/igem/BBa_K1390019/annotation2412361/range2412361/1
component: BBa_K1390005

https://synbiohub.org/public/igem/BBa_K1390019/annotation2412361/range2412361/1
type: Range
persistent identity: range2412361
display id: range2412361
version: 1
direction: direction/reverse
top level: BBa_K1390019
start: 1694
end: 2863
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2412384
display id: annotation2412384
version: 1
top level: BBa_K1390019
location: https://synbiohub.org/public/igem/BBa_K1390019/annotation2412384/range2412384/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K1390019/annotation2412384/range2412384/1
type: Range
persistent identity: range2412384
display id: range2412384
version: 1
direction: direction/reverse
top level: BBa_K1390019
start: 5275
end: 5403
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation2412375
display id: annotation2412375
version: 1
top level: BBa_K1390019
location: https://synbiohub.org/public/igem/BBa_K1390019/annotation2412375/range2412375/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_K1390019/annotation2412375/range2412375/1
type: Range
persistent identity: range2412375
display id: range2412375
version: 1
direction: direction/forward
top level: BBa_K1390019
start: 4201
end: 4213
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation2412367
display id: annotation2412367
version: 1
top level: BBa_K1390019
location: https://synbiohub.org/public/igem/BBa_K1390019/annotation2412367/range2412367/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_K1390019/annotation2412367/range2412367/1
type: Range
persistent identity: range2412367
display id: range2412367
version: 1
direction: direction/forward
top level: BBa_K1390019
start: 3325
end: 3337
orientation: inline

BBa_K1390003
type: SequenceAnnotation
persistent identity: annotation2412373
display id: annotation2412373
version: 1
top level: BBa_K1390019
location: https://synbiohub.org/public/igem/BBa_K1390019/annotation2412373/range2412373/1
component: BBa_K1390003

https://synbiohub.org/public/igem/BBa_K1390019/annotation2412373/range2412373/1
type: Range
persistent identity: range2412373
display id: range2412373
version: 1
direction: direction/reverse
top level: BBa_K1390019
start: 3881
end: 4192
orientation: inline

BBa_K1390004
type: SequenceAnnotation
persistent identity: annotation2412357
display id: annotation2412357
version: 1
top level: BBa_K1390019
location: https://synbiohub.org/public/igem/BBa_K1390019/annotation2412357/range2412357/1
component: BBa_K1390004

https://synbiohub.org/public/igem/BBa_K1390019/annotation2412357/range2412357/1
type: Range
persistent identity: range2412357
display id: range2412357
version: 1
direction: direction/reverse
top level: BBa_K1390019
start: 83
end: 1666
orientation: inline

BBa_J23080
type: ComponentDefinition
persistent identity: BBa J23080
display id: BBa_J23080
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: b0034 derived RBS to match spacing of J01122
creator: bryan hernandez
created: 2006-09-20T11:00:00Z
modified: 2015-08-31T04:08:40Z
discontinued: false
dominant: false
experience: experience/None
group u list: _52_
m user id: 0
owner id: 936
owning group id: 52
sample status: In stock
status: status/Available
bookmark: false
mutable description: RBS spacing modification to [RBS] such that the spacing between the shine-delgarno and the ORF is the same as it is in J01122.
mutable notes: none
mutable provenance: j01122
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J23080_sequence/1

BBa_I732021
type: ComponentDefinition
persistent identity: BBa I732021
display id: BBa_I732021
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Template for Building Primer Family Member
creator: Zhan Jian
created: 2007-07-26T11:00:00Z
modified: 2015-08-31T04:07:56Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1572
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: consensus elements
sequence annotation: -35
sequence annotation: -10
sequence annotation: +1
sequence: https://synbiohub.org/public/igem/BBa_I732021_sequence/1

consensus elements
type: SequenceAnnotation
persistent identity: annotation1939930
display id: annotation1939930
version: 1
top level: BBa_I732021
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_I732021/annotation1939930/range1939930/1

https://synbiohub.org/public/igem/BBa_I732021/annotation1939930/range1939930/1
type: Range
persistent identity: range1939930
display id: range1939930
version: 1
direction: direction/forward
top level: BBa_I732021
start: 62
end: 98
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1939927
display id: annotation1939927
version: 1
top level: BBa_I732021
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I732021/annotation1939927/range1939927/1

https://synbiohub.org/public/igem/BBa_I732021/annotation1939927/range1939927/1
type: Range
persistent identity: range1939927
display id: range1939927
version: 1
direction: direction/forward
top level: BBa_I732021
start: 62
end: 67
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1939928
display id: annotation1939928
version: 1
top level: BBa_I732021
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I732021/annotation1939928/range1939928/1

https://synbiohub.org/public/igem/BBa_I732021/annotation1939928/range1939928/1
type: Range
persistent identity: range1939928
display id: range1939928
version: 1
direction: direction/forward
top level: BBa_I732021
start: 86
end: 91
orientation: inline

+1
type: SequenceAnnotation
persistent identity: annotation1939929
display id: annotation1939929
version: 1
top level: BBa_I732021
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I732021/annotation1939929/range1939929/1

https://synbiohub.org/public/igem/BBa_I732021/annotation1939929/range1939929/1
type: Range
persistent identity: range1939929
display id: range1939929
version: 1
direction: direction/forward
top level: BBa_I732021
start: 98
end: 98
orientation: inline

BBa_M13103
type: ComponentDefinition
persistent identity: BBa M13103
display id: BBa_M13103
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: M13K07 gene III promoter
creator: Natalie Kuldell
created: 2006-12-15T12:00:00Z
modified: 2015-05-08T01:13:55Z
discontinued: false
dominant: true
group u list: _45_
m user id: 0
owner id: 314
owning group id: 1
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This part is from the bacteriophage M13 genome, bp 1500-1547 in M13K07. It directs transcription of M13 gene III (BBa_M13503, BBa_M13003). Its identity as a promoter was described in Gene (1980) 11:129-148 based on -10,-35 homology and fragment of genome able to bind RNAP.
mutable notes: none
mutable provenance: New England Biolabs M13K07. Sequenced in 2002 by L. Panganaban.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_M13103_sequence/1

BBa_K1468002
type: ComponentDefinition
persistent identity: BBa K1468002
display id: BBa_K1468002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pJ23110 + gene encoding ZsGreen
creator: Pedro Luis Dorado Morales
created: 2014-10-02T11:00:00Z
modified: 2015-05-08T01:10:38Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1847_
m user id: 0
owner id: 11756
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: -
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence: https://synbiohub.org/public/igem/BBa_K1468002_sequence/1

Kat
type: ComponentDefinition
persistent identity: BBa I14034
display id: BBa_I14034
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: P(Kat)
creator: Vikram Vijayan, Allen Hsu, Lawrence Fomundam
created: 2004-08-03T11:00:00Z
modified: 2015-08-31T04:07:38Z
discontinued: false
dominant: false
experience: experience/None
group u list: _4_
m user id: 0
owner id: 171
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Constitutive Promoter, Medium Transcription
mutable provenance: nnpp promoter 3 from pSB2K3
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: P(Kat)
sequence: https://synbiohub.org/public/igem/BBa_I14034_sequence/1

P(Kat)
type: SequenceAnnotation
persistent identity: annotation1023116
display id: annotation1023116
version: 1
top level: Kat
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I14034/annotation1023116/range1023116/1

https://synbiohub.org/public/igem/BBa_I14034/annotation1023116/range1023116/1
type: Range
persistent identity: range1023116
display id: range1023116
version: 1
direction: direction/forward
top level: Kat
start: 1
end: 45
orientation: inline

BBa_J61135
type: ComponentDefinition
persistent identity: BBa J61135
display id: BBa_J61135
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ribosome Binding Site Family Member
creator: John Anderson
created: 2007-04-24T11:00:00Z
modified: 2015-08-31T01:59:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: {{JCA_Arkin_RBSFamily}}
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J61135_sequence/1

BBa_J64031
type: ComponentDefinition
persistent identity: BBa J64031
display id: BBa_J64031
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: sptP RBS
creator: Dan Widmaier
created: 2008-01-16T12:00:00Z
modified: 2015-08-31T01:56:26Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _98_
m user id: 0
owner id: 2407
owning group id: 98
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is the natural RBS for the sptP gene in Salmonella Type III Secretion
mutable notes: This part was designed without consideration for biobrick standard assembly. This RBS has not been explicitly tested for expression rate.
mutable provenance: Salmonella LT2 Genomic DNA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: sptP RBS
sequence: https://synbiohub.org/public/igem/BBa_J64031_sequence/1

sptP RBS
type: SequenceAnnotation
persistent identity: annotation1959635
display id: annotation1959635
version: 1
top level: BBa_J64031
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_J64031/annotation1959635/range1959635/1

https://synbiohub.org/public/igem/BBa_J64031/annotation1959635/range1959635/1
type: Range
persistent identity: range1959635
display id: range1959635
version: 1
direction: direction/forward
top level: BBa_J64031
start: 1
end: 20
orientation: inline

BBa_K823011
type: ComponentDefinition
persistent identity: BBa K823011
display id: BBa_K823011
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Anderson promoter J23114
creator: Korinna Kraft
created: 2012-09-07T11:00:00Z
modified: 2015-07-07T11:50:55Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1081_
m user id: 4206
owner id: 12081
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Anderson promoter J23114 without RFP in backbone vector pSB1C3 to easily fuse the promoter with other reporters e.g. the lux operon.
mutable notes: no considerations
mutable provenance: Partsregistry
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: J23114
sequence annotation: -35 box
sequence annotation: -10 box
sequence: https://synbiohub.org/public/igem/BBa_K823011_sequence/1

J23114
type: SequenceAnnotation
persistent identity: annotation2182537
display id: annotation2182537
version: 1
top level: BBa_K823011
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K823011/annotation2182537/range2182537/1

https://synbiohub.org/public/igem/BBa_K823011/annotation2182537/range2182537/1
type: Range
persistent identity: range2182537
display id: range2182537
version: 1
direction: direction/forward
top level: BBa_K823011
start: 1
end: 35
orientation: inline

-35 box
type: SequenceAnnotation
persistent identity: annotation2182538
display id: annotation2182538
version: 1
top level: BBa_K823011
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K823011/annotation2182538/range2182538/1

https://synbiohub.org/public/igem/BBa_K823011/annotation2182538/range2182538/1
type: Range
persistent identity: range2182538
display id: range2182538
version: 1
direction: direction/forward
top level: BBa_K823011
start: 1
end: 6
orientation: inline

-10 box
type: SequenceAnnotation
persistent identity: annotation2182539
display id: annotation2182539
version: 1
top level: BBa_K823011
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K823011/annotation2182539/range2182539/1

https://synbiohub.org/public/igem/BBa_K823011/annotation2182539/range2182539/1
type: Range
persistent identity: range2182539
display id: range2182539
version: 1
direction: direction/forward
top level: BBa_K823011
start: 24
end: 29
orientation: inline

BBa_K823009
type: ComponentDefinition
persistent identity: BBa K823009
display id: BBa_K823009
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Anderson promoter J23107
creator: Korinna Kraft
created: 2012-09-07T11:00:00Z
modified: 2015-05-08T01:13:30Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1081_
m user id: 0
owner id: 12081
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Anderson promoter J23107 (with one nucleotide different to the original Anderson promoter sequence) without RFP in backbone vector pSB1C3 to easily fuse the promoter with other reporters e.g. the lux operon.
mutable notes: no considerations
mutable provenance: Partsregistry
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -35 box
sequence annotation: -10 box
sequence annotation: J23107
sequence: https://synbiohub.org/public/igem/BBa_K823009_sequence/1

-35 box
type: SequenceAnnotation
persistent identity: annotation2182532
display id: annotation2182532
version: 1
top level: BBa_K823009
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K823009/annotation2182532/range2182532/1

https://synbiohub.org/public/igem/BBa_K823009/annotation2182532/range2182532/1
type: Range
persistent identity: range2182532
display id: range2182532
version: 1
direction: direction/forward
top level: BBa_K823009
start: 1
end: 6
orientation: inline

-10 box
type: SequenceAnnotation
persistent identity: annotation2182533
display id: annotation2182533
version: 1
top level: BBa_K823009
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K823009/annotation2182533/range2182533/1

https://synbiohub.org/public/igem/BBa_K823009/annotation2182533/range2182533/1
type: Range
persistent identity: range2182533
display id: range2182533
version: 1
direction: direction/forward
top level: BBa_K823009
start: 24
end: 29
orientation: inline

J23107
type: SequenceAnnotation
persistent identity: annotation2182531
display id: annotation2182531
version: 1
top level: BBa_K823009
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K823009/annotation2182531/range2182531/1

https://synbiohub.org/public/igem/BBa_K823009/annotation2182531/range2182531/1
type: Range
persistent identity: range2182531
display id: range2182531
version: 1
direction: direction/forward
top level: BBa_K823009
start: 1
end: 35
orientation: inline

BBa_K1585102
type: ComponentDefinition
persistent identity: BBa K1585102
display id: BBa_K1585102
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Anderson Promoter with lacI binding site
creator: Tobias Schwanemann
created: 2015-08-31T11:00:00Z
modified: 2015-09-01T02:03:57Z
discontinued: false
dominant: false
experience: experience/Fails
group u list: _2002_
m user id: 24474
owner id: 24474
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Promoters of the Anderson Promoter Collection with lacI binding site derived from [http://parts.igem.org/Part:BBa_R0010 R_0010] to make them [http://openwetware.org/wiki/IPTG IPTG] iducible.
mutable notes: We choosed lacI-binding-site of part BBa_R0010 because we assumed older parts would be more reliable. The part is shipped in backbone [http://parts.igem.org/Part:BBa_J61002 J61002] because the functionality is directly visible by adding IPTG. RFP ([http://parts.igem.org/Part:BBa_E1010 E1010]) should get expressed then.
mutable provenance: Assambly of [http://parts.igem.org/Part:BBa_J23102 J23102] and lacI-binding-site of [http://parts.igem.org/Part:BBa_R0010 R_0010] The "lacI binding site" of Part [http://parts.igem.org/Part:BBa_R0010 R_0010] differs from lacO from Part [http://parts.igem.org/Part:BBa_J33207 BBa_J33207].
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
component: BBa_J23102
component: BBa_K1585999
sequence annotation: BBa_J23102
sequence annotation: BBa_K1585999
sequence: https://synbiohub.org/public/igem/BBa_K1585102_sequence/1

BBa_J23102
type: Component
persistent identity: component2441417
display id: component2441417
version: 1
top level: BBa_K1585102
access: public
definition: BBa_J23102

BBa_K1585999
type: Component
persistent identity: component2441419
display id: component2441419
version: 1
top level: BBa_K1585102
access: public
definition: https://synbiohub.org/public/igem/BBa_K1585999/1

BBa_J23102
type: SequenceAnnotation
persistent identity: annotation2441417
display id: annotation2441417
version: 1
top level: BBa_K1585102
location: https://synbiohub.org/public/igem/BBa_K1585102/annotation2441417/range2441417/1
component: BBa_J23102

https://synbiohub.org/public/igem/BBa_K1585102/annotation2441417/range2441417/1
type: Range
persistent identity: range2441417
display id: range2441417
version: 1
direction: direction/reverse
top level: BBa_K1585102
start: 1
end: 35
orientation: inline

BBa_K1585999
type: SequenceAnnotation
persistent identity: annotation2441419
display id: annotation2441419
version: 1
top level: BBa_K1585102
location: https://synbiohub.org/public/igem/BBa_K1585102/annotation2441419/range2441419/1
component: BBa_K1585999

https://synbiohub.org/public/igem/BBa_K1585102/annotation2441419/range2441419/1
type: Range
persistent identity: range2441419
display id: range2441419
version: 1
direction: direction/reverse
top level: BBa_K1585102
start: 44
end: 78
orientation: inline

BBa_I742146
type: ComponentDefinition
persistent identity: BBa I742146
display id: BBa_I742146
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: sam8 native rbs
creator: Chris French
created: 2007-10-23T11:00:00Z
modified: 2015-08-31T04:08:03Z
discontinued: false
dominant: false
experience: experience/None
group u list: _123_
m user id: 0
owner id: 837
owning group id: 163
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Native ribosome binding site of sam8 (tyrosine ammonia lyase) from the bacterium Saccharothrix espanaensis DSM 44229.
mutable notes: Virtual part to be added to coding sequence I742142.
mutable provenance: Saccharothrix espanaensis DSM 44229.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_I742146_sequence/1

BBa_K823007
type: ComponentDefinition
persistent identity: BBa K823007
display id: BBa_K823007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Anderson promoter J23103
creator: Korinna Kraft
created: 2012-09-07T11:00:00Z
modified: 2015-07-07T11:46:24Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1081_
m user id: 4206
owner id: 12081
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Anderson promoter J23103 without RFP in backbone vector pSB1C3 to easily fuse the promoter with other reporters e.g. the lux operon.
mutable notes: no considerations
mutable provenance: Partsregistry
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -35 box
sequence annotation: -10 box
sequence annotation: J23103
sequence: https://synbiohub.org/public/igem/BBa_K823007_sequence/1

-35 box
type: SequenceAnnotation
persistent identity: annotation2182516
display id: annotation2182516
version: 1
top level: BBa_K823007
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K823007/annotation2182516/range2182516/1

https://synbiohub.org/public/igem/BBa_K823007/annotation2182516/range2182516/1
type: Range
persistent identity: range2182516
display id: range2182516
version: 1
direction: direction/forward
top level: BBa_K823007
start: 1
end: 6
orientation: inline

-10 box
type: SequenceAnnotation
persistent identity: annotation2182517
display id: annotation2182517
version: 1
top level: BBa_K823007
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K823007/annotation2182517/range2182517/1

https://synbiohub.org/public/igem/BBa_K823007/annotation2182517/range2182517/1
type: Range
persistent identity: range2182517
display id: range2182517
version: 1
direction: direction/forward
top level: BBa_K823007
start: 24
end: 29
orientation: inline

J23103
type: SequenceAnnotation
persistent identity: annotation2182515
display id: annotation2182515
version: 1
top level: BBa_K823007
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K823007/annotation2182515/range2182515/1

https://synbiohub.org/public/igem/BBa_K823007/annotation2182515/range2182515/1
type: Range
persistent identity: range2182515
display id: range2182515
version: 1
direction: direction/forward
top level: BBa_K823007
start: 1
end: 35
orientation: inline

CreAp
type: ComponentDefinition
persistent identity: BBa J64951
display id: BBa_J64951
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: E. Coli CreABCD phosphate sensing operon promoter
creator: reid williams
created: 2007-03-19T12:00:00Z
modified: 2015-05-08T01:08:17Z
discontinued: false
dominant: false
experience: experience/None
group u list: _98_
m user id: 0
owner id: 1432
owning group id: 98
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: CreABCD phosphate sensing operon promoter taken from RegulonDB.
mutable notes: none
mutable provenance: E. Coli K12
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J64951_sequence/1

BBa_J44001
type: ComponentDefinition
persistent identity: BBa J44001
display id: BBa_J44001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Reverse RBS (RBSrev) -- corresponds to BBa_B0030
creator: Todd Eckdahl
created: 2006-08-01T11:00:00Z
modified: 2015-08-31T04:08:48Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _61_71_
m user id: 0
owner id: 606
owning group id: 61
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: Reverse version of RBS BBa_B0030
mutable notes: Repeats in oligos caused unusual products during cloning
mutable provenance: Cloned from synthetic oligos
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: Reverse RBS
sequence: https://synbiohub.org/public/igem/BBa_J44001_sequence/1

Reverse RBS
type: SequenceAnnotation
persistent identity: annotation1893199
display id: annotation1893199
version: 1
top level: BBa_J44001
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_J44001/annotation1893199/range1893199/1

https://synbiohub.org/public/igem/BBa_J44001/annotation1893199/range1893199/1
type: Range
persistent identity: range1893199
display id: range1893199
version: 1
direction: direction/reverse
top level: BBa_J44001
start: 1
end: 15
orientation: inline

BBa_Z0262
type: ComponentDefinition
persistent identity: BBa Z0262
display id: BBa_Z0262
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Medium strength T7.2 RBS
creator: Sriram Kosuri
created: 2006-05-15T11:00:00Z
modified: 2015-05-08T01:14:55Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_10_
m user id: 0
owner id: 64
owning group id: 10
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Sequence comes from the natural B0030. Using B0030 as a medium strength RBS is from initial results from experiments characterizing all natural T7 RBSs.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_Z0262_sequence/1

BBa_J61141
type: ComponentDefinition
persistent identity: BBa J61141
display id: BBa_J61141
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: {rbs1} Library Member in BBb
creator: John Anderson
created: 2007-09-03T11:00:00Z
modified: 2015-08-31T01:59:49Z
discontinued: false
dominant: false
experience: experience/None
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: {{BBbRBS1Family}}
mutable notes: {{BerkiGEM2007-Biobrick2.0Intro}}
mutable provenance: Synthetic
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J61141_sequence/1

const.
type: ComponentDefinition
persistent identity: BBa R1074
display id: BBa_R1074
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Constitutive Promoter I
creator: Chris Walsh (Fighting Darwins)
created: 2004-01-27T12:00:00Z
modified: 2015-05-08T01:14:15Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: constitutive promoter which outputs .3 TIPs point mutants of wild type rrnB promoter P1
mutable provenance: Gaal, Gourse, et al.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: Help:Barcodes
sequence annotation: -10
sequence annotation: -35
sequence: https://synbiohub.org/public/igem/BBa_R1074_sequence/1

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2214002
display id: annotation2214002
version: 1
top level: const.
role: SO:0000807
role: feature/barcode
location: https://synbiohub.org/public/igem/BBa_R1074/annotation2214002/range2214002/1

https://synbiohub.org/public/igem/BBa_R1074/annotation2214002/range2214002/1
type: Range
persistent identity: range2214002
display id: range2214002
version: 1
direction: direction/forward
top level: const.
start: 50
end: 74
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation309011
display id: annotation309011
version: 1
top level: const.
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R1074/annotation309011/range309011/1

https://synbiohub.org/public/igem/BBa_R1074/annotation309011/range309011/1
type: Range
persistent identity: range309011
display id: range309011
version: 1
direction: direction/forward
top level: const.
start: 35
end: 40
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation309012
display id: annotation309012
version: 1
top level: const.
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R1074/annotation309012/range309012/1

https://synbiohub.org/public/igem/BBa_R1074/annotation309012/range309012/1
type: Range
persistent identity: range309012
display id: range309012
version: 1
direction: direction/forward
top level: const.
start: 15
end: 20
orientation: inline

BBa_J23113
type: ComponentDefinition
persistent identity: BBa J23113
display id: BBa_J23113
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: constitutive promoter family member
creator: John Anderson
created: 2006-08-16T11:00:00Z
modified: 2015-08-31T04:08:40Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: Later
mutable notes: N/A
mutable provenance: Later
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J23113_sequence/1

BBa_J64020
type: ComponentDefinition
persistent identity: BBa J64020
display id: BBa_J64020
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: invI RBS
creator: Dan Widmaier
created: 2008-01-16T12:00:00Z
modified: 2015-08-31T01:56:26Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _98_
m user id: 0
owner id: 2407
owning group id: 98
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This part is the natural invI RBS from Salmonella Typhimurium LT2.
mutable notes: This part was not designed with consideration for biobrick standard assembly in mind. This RBS has not been explicitly tested for expression level in a fluorescence or B-Gal assay.
mutable provenance: Salmonella Typhimurium LT2 Genomic DNA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: invI RBS
sequence: https://synbiohub.org/public/igem/BBa_J64020_sequence/1

invI RBS
type: SequenceAnnotation
persistent identity: annotation1959619
display id: annotation1959619
version: 1
top level: BBa_J64020
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_J64020/annotation1959619/range1959619/1

https://synbiohub.org/public/igem/BBa_J64020/annotation1959619/range1959619/1
type: Range
persistent identity: range1959619
display id: range1959619
version: 1
direction: direction/forward
top level: BBa_J64020
start: 1
end: 20
orientation: inline

BBa_M13510
type: ComponentDefinition
persistent identity: BBa M13510
display id: BBa_M13510
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: M13KO7 gene X RBS
creator: Natalie Kuldell
created: 2006-12-14T12:00:00Z
modified: 2015-05-08T01:13:56Z
discontinued: false
dominant: false
experience: experience/None
group u list: _45_
m user id: 0
owner id: 314
owning group id: 1
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This RBS is part of the bacteriophage M13 genome, initiating translation of the gene X message (BBa_M13010). The part aligns with base pairs 480-495 in M13K07 genome. It was identified as the RBS for gene II message by Wezenbeek et al in Gene (1980) 11: 129-148
mutable notes: none
mutable provenance: New England Biolabs M13K07. Sequenced in 2002 by L. Panganaban
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_M13510_sequence/1

BBa_J34801
type: ComponentDefinition
persistent identity: BBa J34801
display id: BBa_J34801
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: ribosome binding site
creator: Alexandra Choutko
created: 2006-10-25T11:00:00Z
modified: 2015-08-31T04:08:46Z
discontinued: false
dominant: false
experience: experience/None
group u list: _62_
m user id: 0
owner id: 1085
owning group id: 62
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: a
mutable notes: a
mutable provenance: a
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J34801_sequence/1

BBa_K256033
type: ComponentDefinition
persistent identity: BBa K256033
display id: BBa_K256033
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Infrared signal reporter (J23119:IFP:J23119:HO1)
creator: iGEM09_NTU-Singapore
created: 2009-10-19T11:00:00Z
modified: 2015-05-08T01:11:41Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _359_
m user id: 0
owner id: 4864
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This part co-expresses IFP and HO1.
mutable notes: --
mutable provenance: Gene sequence is obtained directly from Tsien's lab.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_K256020
component: BBa_K256018
sequence annotation: BBa_K256020
sequence annotation: BBa_K256018
sequence: https://synbiohub.org/public/igem/BBa_K256033_sequence/1

BBa_K256020
type: Component
persistent identity: component2288213
display id: component2288213
version: 1
top level: BBa_K256033
access: public
definition: BBa_K256020

BBa_K256018
type: Component
persistent identity: component2288200
display id: component2288200
version: 1
top level: BBa_K256033
access: public
definition: BBa_K256018

BBa_K256020
type: SequenceAnnotation
persistent identity: annotation2288213
display id: annotation2288213
version: 1
top level: BBa_K256033
location: https://synbiohub.org/public/igem/BBa_K256033/annotation2288213/range2288213/1
component: BBa_K256020

https://synbiohub.org/public/igem/BBa_K256033/annotation2288213/range2288213/1
type: Range
persistent identity: range2288213
display id: range2288213
version: 1
direction: direction/reverse
top level: BBa_K256033
start: 1176
end: 2124
orientation: inline

BBa_K256018
type: SequenceAnnotation
persistent identity: annotation2288200
display id: annotation2288200
version: 1
top level: BBa_K256033
location: https://synbiohub.org/public/igem/BBa_K256033/annotation2288200/range2288200/1
component: BBa_K256018

https://synbiohub.org/public/igem/BBa_K256033/annotation2288200/range2288200/1
type: Range
persistent identity: range2288200
display id: range2288200
version: 1
direction: direction/reverse
top level: BBa_K256033
start: 1
end: 1167
orientation: inline

BBa_J61139
type: ComponentDefinition
persistent identity: BBa J61139
display id: BBa_J61139
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ribosome Binding Site Family Member
creator: John Anderson
created: 2007-04-24T11:00:00Z
modified: 2015-08-31T01:59:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable description: {{JCA_Arkin_RBSFamily}}
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J61139_sequence/1

BBa_J61105
type: ComponentDefinition
persistent identity: BBa J61105
display id: BBa_J61105
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ribosome Binding Site Family Member
creator: John Anderson
created: 2007-04-23T11:00:00Z
modified: 2015-08-31T02:03:00Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable description: {{JCA_Arkin_RBSFamily}}
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J61105_sequence/1

BBa_K608351
type: ComponentDefinition
persistent identity: BBa K608351
display id: BBa_K608351
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: (correct) temperature sensitive promoter
creator: Theodhor Tiko
created: 2011-09-13T11:00:00Z
modified: 2015-05-08T01:12:52Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _780_
m user id: 0
owner id: 9113
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: correct temperatursesitive promotor
mutable notes: asd
mutable provenance: asd
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: conserved
sequence annotation: T7 TE
sequence annotation: BBa_J23114
sequence annotation: -10
sequence annotation: OR2
sequence annotation: cIts
sequence annotation: OR1
sequence annotation: BBa_K098997
sequence annotation: BBa_B0012
sequence annotation: polya
sequence annotation: BBa_B0034
sequence annotation: stem_loop
sequence annotation: BBa_R0051
sequence annotation: stop
sequence annotation: -35
sequence annotation: BBa_B0011
sequence: https://synbiohub.org/public/igem/BBa_K608351_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation2131425
display id: annotation2131425
version: 1
top level: BBa_K608351
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K608351/annotation2131425/range2131425/1

https://synbiohub.org/public/igem/BBa_K608351/annotation2131425/range2131425/1
type: Range
persistent identity: range2131425
display id: range2131425
version: 1
direction: direction/forward
top level: BBa_K608351
start: 776
end: 788
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation2131306
display id: annotation2131306
version: 1
top level: BBa_K608351
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K608351/annotation2131306/range2131306/1

https://synbiohub.org/public/igem/BBa_K608351/annotation2131306/range2131306/1
type: Range
persistent identity: range2131306
display id: range2131306
version: 1
direction: direction/forward
top level: BBa_K608351
start: 804
end: 823
orientation: inline

BBa_J23114
type: SequenceAnnotation
persistent identity: annotation2131300
display id: annotation2131300
version: 1
top level: BBa_K608351
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K608351/annotation2131300/range2131300/1

https://synbiohub.org/public/igem/BBa_K608351/annotation2131300/range2131300/1
type: Range
persistent identity: range2131300
display id: range2131300
version: 1
direction: direction/reverse
top level: BBa_K608351
start: 1
end: 35
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2131315
display id: annotation2131315
version: 1
top level: BBa_K608351
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K608351/annotation2131315/range2131315/1

https://synbiohub.org/public/igem/BBa_K608351/annotation2131315/range2131315/1
type: Range
persistent identity: range2131315
display id: range2131315
version: 1
direction: direction/forward
top level: BBa_K608351
start: 937
end: 942
orientation: inline

OR2
type: SequenceAnnotation
persistent identity: annotation2131311
display id: annotation2131311
version: 1
top level: BBa_K608351
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K608351/annotation2131311/range2131311/1

https://synbiohub.org/public/igem/BBa_K608351/annotation2131311/range2131311/1
type: Range
persistent identity: range2131311
display id: range2131311
version: 1
direction: direction/forward
top level: BBa_K608351
start: 900
end: 916
orientation: inline

cIts
type: SequenceAnnotation
persistent identity: annotation2131303
display id: annotation2131303
version: 1
top level: BBa_K608351
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K608351/annotation2131303/range2131303/1

https://synbiohub.org/public/igem/BBa_K608351/annotation2131303/range2131303/1
type: Range
persistent identity: range2131303
display id: range2131303
version: 1
direction: direction/forward
top level: BBa_K608351
start: 62
end: 775
orientation: inline

OR1
type: SequenceAnnotation
persistent identity: annotation2131314
display id: annotation2131314
version: 1
top level: BBa_K608351
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K608351/annotation2131314/range2131314/1

https://synbiohub.org/public/igem/BBa_K608351/annotation2131314/range2131314/1
type: Range
persistent identity: range2131314
display id: range2131314
version: 1
direction: direction/forward
top level: BBa_K608351
start: 924
end: 940
orientation: inline

BBa_K098997
type: SequenceAnnotation
persistent identity: annotation2131304
display id: annotation2131304
version: 1
top level: BBa_K608351
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K608351/annotation2131304/range2131304/1

https://synbiohub.org/public/igem/BBa_K608351/annotation2131304/range2131304/1
type: Range
persistent identity: range2131304
display id: range2131304
version: 1
direction: direction/reverse
top level: BBa_K608351
start: 62
end: 775
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2131305
display id: annotation2131305
version: 1
top level: BBa_K608351
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K608351/annotation2131305/range2131305/1

https://synbiohub.org/public/igem/BBa_K608351/annotation2131305/range2131305/1
type: Range
persistent identity: range2131305
display id: range2131305
version: 1
direction: direction/forward
top level: BBa_K608351
start: 797
end: 837
orientation: inline

conserved
type: SequenceAnnotation
persistent identity: annotation2131301
display id: annotation2131301
version: 1
top level: BBa_K608351
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K608351/annotation2131301/range2131301/1

https://synbiohub.org/public/igem/BBa_K608351/annotation2131301/range2131301/1
type: Range
persistent identity: range2131301
display id: range2131301
version: 1
direction: direction/forward
top level: BBa_K608351
start: 48
end: 51
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation2131307
display id: annotation2131307
version: 1
top level: BBa_K608351
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_K608351/annotation2131307/range2131307/1

https://synbiohub.org/public/igem/BBa_K608351/annotation2131307/range2131307/1
type: Range
persistent identity: range2131307
display id: range2131307
version: 1
direction: direction/forward
top level: BBa_K608351
start: 824
end: 837
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2131302
display id: annotation2131302
version: 1
top level: BBa_K608351
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K608351/annotation2131302/range2131302/1

https://synbiohub.org/public/igem/BBa_K608351/annotation2131302/range2131302/1
type: Range
persistent identity: range2131302
display id: range2131302
version: 1
direction: direction/reverse
top level: BBa_K608351
start: 44
end: 55
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation2131310
display id: annotation2131310
version: 1
top level: BBa_K608351
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K608351/annotation2131310/range2131310/1

https://synbiohub.org/public/igem/BBa_K608351/annotation2131310/range2131310/1
type: Range
persistent identity: range2131310
display id: range2131310
version: 1
direction: direction/forward
top level: BBa_K608351
start: 858
end: 880
orientation: inline

BBa_R0051
type: SequenceAnnotation
persistent identity: annotation2131312
display id: annotation2131312
version: 1
top level: BBa_K608351
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K608351/annotation2131312/range2131312/1

https://synbiohub.org/public/igem/BBa_K608351/annotation2131312/range2131312/1
type: Range
persistent identity: range2131312
display id: range2131312
version: 1
direction: direction/forward
top level: BBa_K608351
start: 900
end: 948
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2131308
display id: annotation2131308
version: 1
top level: BBa_K608351
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K608351/annotation2131308/range2131308/1

https://synbiohub.org/public/igem/BBa_K608351/annotation2131308/range2131308/1
type: Range
persistent identity: range2131308
display id: range2131308
version: 1
direction: direction/forward
top level: BBa_K608351
start: 830
end: 830
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2131313
display id: annotation2131313
version: 1
top level: BBa_K608351
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K608351/annotation2131313/range2131313/1

https://synbiohub.org/public/igem/BBa_K608351/annotation2131313/range2131313/1
type: Range
persistent identity: range2131313
display id: range2131313
version: 1
direction: direction/forward
top level: BBa_K608351
start: 914
end: 919
orientation: inline

BBa_B0011
type: SequenceAnnotation
persistent identity: annotation2131309
display id: annotation2131309
version: 1
top level: BBa_K608351
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K608351/annotation2131309/range2131309/1

https://synbiohub.org/public/igem/BBa_K608351/annotation2131309/range2131309/1
type: Range
persistent identity: range2131309
display id: range2131309
version: 1
direction: direction/forward
top level: BBa_K608351
start: 846
end: 891
orientation: inline

nickname
type: ComponentDefinition
persistent identity: BBa K1479004
display id: BBa_K1479004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Just for upload test
creator: ChenYB
created: 2014-09-30T11:00:00Z
modified: 2015-05-08T01:10:40Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1859_
m user id: 0
owner id: 21515
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This part is just for upload test. It is meaningless
mutable notes: NULL
mutable provenance: NULL
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: BBa_B1007
sequence annotation: PolyA
sequence annotation: T7 TE
sequence annotation: BBa_B0012
sequence annotation: stem_loop
sequence annotation: BBa_B0011
sequence annotation: BBa_B0015
sequence annotation: stop
sequence annotation: BBa_K1479001
sequence annotation: BBa_B0014
sequence annotation: polya
sequence annotation: misc
sequence annotation: BBa_K1479002
sequence annotation: 22
sequence annotation: BBa_B0010
sequence annotation: BBa_B1008
sequence annotation: modified thr terminator
sequence annotation: B1007
sequence annotation: B1008
sequence annotation: modified thr
sequence: https://synbiohub.org/public/igem/BBa_K1479004_sequence/1

BBa_B1007
type: SequenceAnnotation
persistent identity: annotation2397971
display id: annotation2397971
version: 1
top level: nickname
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1479004/annotation2397971/range2397971/1

https://synbiohub.org/public/igem/BBa_K1479004/annotation2397971/range2397971/1
type: Range
persistent identity: range2397971
display id: range2397971
version: 1
direction: direction/reverse
top level: nickname
start: 152
end: 191
orientation: inline

PolyA
type: SequenceAnnotation
persistent identity: annotation2397970
display id: annotation2397970
version: 1
top level: nickname
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_K1479004/annotation2397970/range2397970/1

https://synbiohub.org/public/igem/BBa_K1479004/annotation2397970/range2397970/1
type: Range
persistent identity: range2397970
display id: range2397970
version: 1
direction: direction/forward
top level: nickname
start: 135
end: 139
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation2397960
display id: annotation2397960
version: 1
top level: nickname
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1479004/annotation2397960/range2397960/1

https://synbiohub.org/public/igem/BBa_K1479004/annotation2397960/range2397960/1
type: Range
persistent identity: range2397960
display id: range2397960
version: 1
direction: direction/forward
top level: nickname
start: 8
end: 27
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2397976
display id: annotation2397976
version: 1
top level: nickname
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1479004/annotation2397976/range2397976/1

https://synbiohub.org/public/igem/BBa_K1479004/annotation2397976/range2397976/1
type: Range
persistent identity: range2397976
display id: range2397976
version: 1
direction: direction/forward
top level: nickname
start: 200
end: 240
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation2397982
display id: annotation2397982
version: 1
top level: nickname
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1479004/annotation2397982/range2397982/1

https://synbiohub.org/public/igem/BBa_K1479004/annotation2397982/range2397982/1
type: Range
persistent identity: range2397982
display id: range2397982
version: 1
direction: direction/forward
top level: nickname
start: 261
end: 283
orientation: inline

PolyA
type: SequenceAnnotation
persistent identity: annotation2397968
display id: annotation2397968
version: 1
top level: nickname
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_K1479004/annotation2397968/range2397968/1

https://synbiohub.org/public/igem/BBa_K1479004/annotation2397968/range2397968/1
type: Range
persistent identity: range2397968
display id: range2397968
version: 1
direction: direction/reverse
top level: nickname
start: 108
end: 112
orientation: inline

BBa_B0011
type: SequenceAnnotation
persistent identity: annotation2397963
display id: annotation2397963
version: 1
top level: nickname
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1479004/annotation2397963/range2397963/1

https://synbiohub.org/public/igem/BBa_K1479004/annotation2397963/range2397963/1
type: Range
persistent identity: range2397963
display id: range2397963
version: 1
direction: direction/forward
top level: nickname
start: 50
end: 95
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2397984
display id: annotation2397984
version: 1
top level: nickname
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1479004/annotation2397984/range2397984/1

https://synbiohub.org/public/igem/BBa_K1479004/annotation2397984/range2397984/1
type: Range
persistent identity: range2397984
display id: range2397984
version: 1
direction: direction/reverse
top level: nickname
start: 303
end: 431
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2397989
display id: annotation2397989
version: 1
top level: nickname
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1479004/annotation2397989/range2397989/1

https://synbiohub.org/public/igem/BBa_K1479004/annotation2397989/range2397989/1
type: Range
persistent identity: range2397989
display id: range2397989
version: 1
direction: direction/forward
top level: nickname
start: 424
end: 424
orientation: inline

BBa_K1479001
type: SequenceAnnotation
persistent identity: annotation2397965
display id: annotation2397965
version: 1
top level: nickname
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1479004/annotation2397965/range2397965/1

https://synbiohub.org/public/igem/BBa_K1479004/annotation2397965/range2397965/1
type: Range
persistent identity: range2397965
display id: range2397965
version: 1
direction: direction/reverse
top level: nickname
start: 104
end: 431
orientation: inline

BBa_B0014
type: SequenceAnnotation
persistent identity: annotation2397977
display id: annotation2397977
version: 1
top level: nickname
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1479004/annotation2397977/range2397977/1

https://synbiohub.org/public/igem/BBa_K1479004/annotation2397977/range2397977/1
type: Range
persistent identity: range2397977
display id: range2397977
version: 1
direction: direction/reverse
top level: nickname
start: 200
end: 294
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation2397979
display id: annotation2397979
version: 1
top level: nickname
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_K1479004/annotation2397979/range2397979/1

https://synbiohub.org/public/igem/BBa_K1479004/annotation2397979/range2397979/1
type: Range
persistent identity: range2397979
display id: range2397979
version: 1
direction: direction/forward
top level: nickname
start: 227
end: 240
orientation: inline

misc
type: SequenceAnnotation
persistent identity: annotation2397990
display id: annotation2397990
version: 1
top level: nickname
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1479004/annotation2397990/range2397990/1

https://synbiohub.org/public/igem/BBa_K1479004/annotation2397990/range2397990/1
type: Range
persistent identity: range2397990
display id: range2397990
version: 1
direction: direction/reverse
top level: nickname
start: 1
end: 20
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2397986
display id: annotation2397986
version: 1
top level: nickname
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1479004/annotation2397986/range2397986/1

https://synbiohub.org/public/igem/BBa_K1479004/annotation2397986/range2397986/1
type: Range
persistent identity: range2397986
display id: range2397986
version: 1
direction: direction/forward
top level: nickname
start: 391
end: 431
orientation: inline

BBa_K1479002
type: SequenceAnnotation
persistent identity: annotation2397955
display id: annotation2397955
version: 1
top level: nickname
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1479004/annotation2397955/range2397955/1

https://synbiohub.org/public/igem/BBa_K1479004/annotation2397955/range2397955/1
type: Range
persistent identity: range2397955
display id: range2397955
version: 1
direction: direction/reverse
top level: nickname
start: 1
end: 95
orientation: inline

PolyA
type: SequenceAnnotation
persistent identity: annotation2397975
display id: annotation2397975
version: 1
top level: nickname
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_K1479004/annotation2397975/range2397975/1

https://synbiohub.org/public/igem/BBa_K1479004/annotation2397975/range2397975/1
type: Range
persistent identity: range2397975
display id: range2397975
version: 1
direction: direction/forward
top level: nickname
start: 183
end: 188
orientation: inline

BBa_B0011
type: SequenceAnnotation
persistent identity: annotation2397981
display id: annotation2397981
version: 1
top level: nickname
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1479004/annotation2397981/range2397981/1

https://synbiohub.org/public/igem/BBa_K1479004/annotation2397981/range2397981/1
type: Range
persistent identity: range2397981
display id: range2397981
version: 1
direction: direction/forward
top level: nickname
start: 249
end: 294
orientation: inline

22
type: SequenceAnnotation
persistent identity: annotation2397957
display id: annotation2397957
version: 1
top level: nickname
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1479004/annotation2397957/range2397957/1

https://synbiohub.org/public/igem/BBa_K1479004/annotation2397957/range2397957/1
type: Range
persistent identity: range2397957
display id: range2397957
version: 1
direction: direction/forward
top level: nickname
start: 1
end: 10
orientation: inline

misc
type: SequenceAnnotation
persistent identity: annotation2397956
display id: annotation2397956
version: 1
top level: nickname
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1479004/annotation2397956/range2397956/1

https://synbiohub.org/public/igem/BBa_K1479004/annotation2397956/range2397956/1
type: Range
persistent identity: range2397956
display id: range2397956
version: 1
direction: direction/forward
top level: nickname
start: 1
end: 20
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2397962
display id: annotation2397962
version: 1
top level: nickname
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1479004/annotation2397962/range2397962/1

https://synbiohub.org/public/igem/BBa_K1479004/annotation2397962/range2397962/1
type: Range
persistent identity: range2397962
display id: range2397962
version: 1
direction: direction/forward
top level: nickname
start: 34
end: 34
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation2397985
display id: annotation2397985
version: 1
top level: nickname
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1479004/annotation2397985/range2397985/1

https://synbiohub.org/public/igem/BBa_K1479004/annotation2397985/range2397985/1
type: Range
persistent identity: range2397985
display id: range2397985
version: 1
direction: direction/forward
top level: nickname
start: 314
end: 357
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation2397978
display id: annotation2397978
version: 1
top level: nickname
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1479004/annotation2397978/range2397978/1

https://synbiohub.org/public/igem/BBa_K1479004/annotation2397978/range2397978/1
type: Range
persistent identity: range2397978
display id: range2397978
version: 1
direction: direction/forward
top level: nickname
start: 207
end: 226
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2397983
display id: annotation2397983
version: 1
top level: nickname
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1479004/annotation2397983/range2397983/1

https://synbiohub.org/public/igem/BBa_K1479004/annotation2397983/range2397983/1
type: Range
persistent identity: range2397983
display id: range2397983
version: 1
direction: direction/forward
top level: nickname
start: 303
end: 382
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation2397964
display id: annotation2397964
version: 1
top level: nickname
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1479004/annotation2397964/range2397964/1

https://synbiohub.org/public/igem/BBa_K1479004/annotation2397964/range2397964/1
type: Range
persistent identity: range2397964
display id: range2397964
version: 1
direction: direction/forward
top level: nickname
start: 62
end: 84
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2397959
display id: annotation2397959
version: 1
top level: nickname
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1479004/annotation2397959/range2397959/1

https://synbiohub.org/public/igem/BBa_K1479004/annotation2397959/range2397959/1
type: Range
persistent identity: range2397959
display id: range2397959
version: 1
direction: direction/forward
top level: nickname
start: 1
end: 41
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation2397988
display id: annotation2397988
version: 1
top level: nickname
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_K1479004/annotation2397988/range2397988/1

https://synbiohub.org/public/igem/BBa_K1479004/annotation2397988/range2397988/1
type: Range
persistent identity: range2397988
display id: range2397988
version: 1
direction: direction/forward
top level: nickname
start: 418
end: 431
orientation: inline

BBa_B1008
type: SequenceAnnotation
persistent identity: annotation2397967
display id: annotation2397967
version: 1
top level: nickname
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1479004/annotation2397967/range2397967/1

https://synbiohub.org/public/igem/BBa_K1479004/annotation2397967/range2397967/1
type: Range
persistent identity: range2397967
display id: range2397967
version: 1
direction: direction/reverse
top level: nickname
start: 104
end: 143
orientation: inline

modified thr terminator
type: SequenceAnnotation
persistent identity: annotation2397969
display id: annotation2397969
version: 1
top level: nickname
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1479004/annotation2397969/range2397969/1

https://synbiohub.org/public/igem/BBa_K1479004/annotation2397969/range2397969/1
type: Range
persistent identity: range2397969
display id: range2397969
version: 1
direction: direction/forward
top level: nickname
start: 113
end: 134
orientation: inline

B1007
type: SequenceAnnotation
persistent identity: annotation2397972
display id: annotation2397972
version: 1
top level: nickname
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1479004/annotation2397972/range2397972/1

https://synbiohub.org/public/igem/BBa_K1479004/annotation2397972/range2397972/1
type: Range
persistent identity: range2397972
display id: range2397972
version: 1
direction: direction/forward
top level: nickname
start: 152
end: 191
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation2397961
display id: annotation2397961
version: 1
top level: nickname
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_K1479004/annotation2397961/range2397961/1

https://synbiohub.org/public/igem/BBa_K1479004/annotation2397961/range2397961/1
type: Range
persistent identity: range2397961
display id: range2397961
version: 1
direction: direction/forward
top level: nickname
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2397980
display id: annotation2397980
version: 1
top level: nickname
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1479004/annotation2397980/range2397980/1

https://synbiohub.org/public/igem/BBa_K1479004/annotation2397980/range2397980/1
type: Range
persistent identity: range2397980
display id: range2397980
version: 1
direction: direction/forward
top level: nickname
start: 233
end: 233
orientation: inline

PolyA
type: SequenceAnnotation
persistent identity: annotation2397973
display id: annotation2397973
version: 1
top level: nickname
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_K1479004/annotation2397973/range2397973/1

https://synbiohub.org/public/igem/BBa_K1479004/annotation2397973/range2397973/1
type: Range
persistent identity: range2397973
display id: range2397973
version: 1
direction: direction/reverse
top level: nickname
start: 155
end: 160
orientation: inline

B1008
type: SequenceAnnotation
persistent identity: annotation2397966
display id: annotation2397966
version: 1
top level: nickname
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1479004/annotation2397966/range2397966/1

https://synbiohub.org/public/igem/BBa_K1479004/annotation2397966/range2397966/1
type: Range
persistent identity: range2397966
display id: range2397966
version: 1
direction: direction/forward
top level: nickname
start: 104
end: 143
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation2397987
display id: annotation2397987
version: 1
top level: nickname
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1479004/annotation2397987/range2397987/1

https://synbiohub.org/public/igem/BBa_K1479004/annotation2397987/range2397987/1
type: Range
persistent identity: range2397987
display id: range2397987
version: 1
direction: direction/forward
top level: nickname
start: 398
end: 417
orientation: inline

modified thr
type: SequenceAnnotation
persistent identity: annotation2397974
display id: annotation2397974
version: 1
top level: nickname
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1479004/annotation2397974/range2397974/1

https://synbiohub.org/public/igem/BBa_K1479004/annotation2397974/range2397974/1
type: Range
persistent identity: range2397974
display id: range2397974
version: 1
direction: direction/forward
top level: nickname
start: 161
end: 182
orientation: inline

BBa_B0076
type: ComponentDefinition
persistent identity: BBa B0076
display id: BBa_B0076
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Specialized RBS
creator: Bartholomew Canton
created: 2006-05-01T11:00:00Z
modified: 2015-08-31T04:07:21Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_6_
m user id: 0
owner id: 135
owning group id: 6
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_B0076_sequence/1

CP2LacPI1
type: ComponentDefinition
persistent identity: BBa K418003
display id: BBa_K418003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: IPTG inducible Lac promoter cassette
creator: Northwestern University iGEM 2010
created: 2010-10-15T11:00:00Z
modified: 2015-05-08T01:12:28Z
discontinued: false
dominant: false
experience: experience/None
group u list: _532_
m user id: 0
owner id: 6938
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_Q01121
component: BBa_J23104
sequence annotation: BBa_Q01121
sequence annotation: BBa_J23104
sequence: https://synbiohub.org/public/igem/BBa_K418003_sequence/1

BBa_Q01121
type: Component
persistent identity: component2263782
display id: component2263782
version: 1
top level: CP2LacPI1
access: public
definition: https://synbiohub.org/public/igem/BBa_Q01121/1

BBa_J23104
type: Component
persistent identity: component2263763
display id: component2263763
version: 1
top level: CP2LacPI1
access: public
definition: BBa_J23104

BBa_Q01121
type: SequenceAnnotation
persistent identity: annotation2263782
display id: annotation2263782
version: 1
top level: CP2LacPI1
location: https://synbiohub.org/public/igem/BBa_K418003/annotation2263782/range2263782/1
component: BBa_Q01121

https://synbiohub.org/public/igem/BBa_K418003/annotation2263782/range2263782/1
type: Range
persistent identity: range2263782
display id: range2263782
version: 1
direction: direction/reverse
top level: CP2LacPI1
start: 44
end: 1416
orientation: inline

BBa_J23104
type: SequenceAnnotation
persistent identity: annotation2263763
display id: annotation2263763
version: 1
top level: CP2LacPI1
location: https://synbiohub.org/public/igem/BBa_K418003/annotation2263763/range2263763/1
component: BBa_J23104

https://synbiohub.org/public/igem/BBa_K418003/annotation2263763/range2263763/1
type: Range
persistent identity: range2263763
display id: range2263763
version: 1
direction: direction/reverse
top level: CP2LacPI1
start: 1
end: 35
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS (Elowitz 1999) -- defines RBS efficiency
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: RBS based on Elowitz repressilator.
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
direction: direction/forward
top level: BBa_B0034
start: 5
end: 8
orientation: inline

BBa_J23106
type: ComponentDefinition
persistent identity: BBa J23106
display id: BBa_J23106
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: constitutive promoter family member
creator: John Anderson
created: 2006-08-13T11:00:00Z
modified: 2015-08-31T04:08:40Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: Later
mutable notes: N/A
mutable provenance: Isolated from library of promoters
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J23106_sequence/1

BBa_J61129
type: ComponentDefinition
persistent identity: BBa J61129
display id: BBa_J61129
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ribosome Binding Site Family Member
creator: John Anderson
created: 2007-04-24T11:00:00Z
modified: 2015-08-31T01:59:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable description: {{JCA_Arkin_RBSFamily}}
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J61129_sequence/1

BBa_J61113
type: ComponentDefinition
persistent identity: BBa J61113
display id: BBa_J61113
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ribosome Binding Site Family Member
creator: John Anderson
created: 2007-04-23T11:00:00Z
modified: 2015-08-31T02:03:00Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable description: {{JCA_Arkin_RBSFamily}}
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J61113_sequence/1

RBS-GsiB
type: ComponentDefinition
persistent identity: BBa K143020
display id: BBa_K143020
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: GsiB ribosome binding site (RBS) for B. subtilis
creator: James Chappell
created: 2008-09-14T11:00:00Z
modified: 2015-05-08T01:10:23Z
discontinued: false
dominant: false
experience: experience/None
group u list: _199_
m user id: 0
owner id: 2090
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: GsiB is an endogenous ribosome binding site from ''B.subtilis''. The sequence of the gsiB ribosome binding site is '''AAAGGAGG''' which is complementary to the sequence '''UUUCCUCC''' from the 3' region of the 16s rRNA from ''B.subtilis''. GsiB is an endogenous ribosome binding site (RBS) from ''B.subtilis''. The sequence of the gsiB ribosome binding site is '''AAAGGAGG''' which is complementary to the sequence '''UUUCCUCC''' from the 3' region of the 16s rRNA from ''B.subtilis''. Previous research showed that the predicted binding energy of the 16s rRNA to the RBS is -9.3kcal.
mutable notes: In order to ensure that the RBS is functional the actual ribosome binding site was maintained and the distance between the RBS and the start codon maintained. In order to conform to the biobrick standard the sequence flanking the RBS had to be changed but the distance between the promoter and RBS, and start codon and RBS was maintained.
mutable provenance: The sequence was taken from a previous research paper [1] and was constructed by Geneart
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: rbs
sequence: https://synbiohub.org/public/igem/BBa_K143020_sequence/1

rbs
type: SequenceAnnotation
persistent identity: annotation1975872
display id: annotation1975872
version: 1
top level: RBS-GsiB
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K143020/annotation1975872/range1975872/1

https://synbiohub.org/public/igem/BBa_K143020/annotation1975872/range1975872/1
type: Range
persistent identity: range1975872
display id: range1975872
version: 1
direction: direction/forward
top level: RBS-GsiB
start: 2
end: 8
orientation: inline

BBa_B2017
type: ComponentDefinition
persistent identity: BBa B2017
display id: BBa_B2017
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: 2.8 RBS from T7
creator: Bartholomew Canton
created: 2007-05-22T11:00:00Z
modified: 2015-08-31T04:07:21Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_
m user id: 0
owner id: 135
owning group id: 84
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: to be added automatically later
mutable notes: to be added automatically later
mutable provenance: to be added automatically later
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_B2017_sequence/1

BBa_K1468004
type: ComponentDefinition
persistent identity: BBa K1468004
display id: BBa_K1468004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pTet + gene encoding LacZ
creator: Pedro Luis Dorado Morales
created: 2014-10-02T11:00:00Z
modified: 2015-05-08T01:10:38Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1847_
m user id: 0
owner id: 11756
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: -
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: BBa_K1468004
sequence annotation: C --> T
sequence annotation: LacZ protein
sequence annotation: TetR 2
sequence annotation: TetR 1
sequence annotation: START
sequence annotation: LacZ
sequence annotation: STOP
sequence annotation: BBa_I732005
sequence annotation: BBa_R0040
sequence annotation: -10
sequence annotation: -35
sequence: https://synbiohub.org/public/igem/BBa_K1468004_sequence/1

BBa_K1468004
type: SequenceAnnotation
persistent identity: annotation2400601
display id: annotation2400601
version: 1
top level: BBa_K1468004
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1468004/annotation2400601/range2400601/1

https://synbiohub.org/public/igem/BBa_K1468004/annotation2400601/range2400601/1
type: Range
persistent identity: range2400601
display id: range2400601
version: 1
direction: direction/forward
top level: BBa_K1468004
start: 1
end: 3129
orientation: inline

C --> T
type: SequenceAnnotation
persistent identity: annotation2400598
display id: annotation2400598
version: 1
top level: BBa_K1468004
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_K1468004/annotation2400598/range2400598/1

https://synbiohub.org/public/igem/BBa_K1468004/annotation2400598/range2400598/1
type: Range
persistent identity: range2400598
display id: range2400598
version: 1
direction: direction/forward
top level: BBa_K1468004
start: 3085
end: 3085
orientation: inline

LacZ protein
type: SequenceAnnotation
persistent identity: annotation2418063
display id: annotation2418063
version: 1
top level: BBa_K1468004
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1468004/annotation2418063/range2418063/1

https://synbiohub.org/public/igem/BBa_K1468004/annotation2418063/range2418063/1
type: Range
persistent identity: range2418063
display id: range2418063
version: 1
direction: direction/forward
top level: BBa_K1468004
start: 61
end: 3129
orientation: inline

TetR 2
type: SequenceAnnotation
persistent identity: annotation2400594
display id: annotation2400594
version: 1
top level: BBa_K1468004
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1468004/annotation2400594/range2400594/1

https://synbiohub.org/public/igem/BBa_K1468004/annotation2400594/range2400594/1
type: Range
persistent identity: range2400594
display id: range2400594
version: 1
direction: direction/forward
top level: BBa_K1468004
start: 26
end: 44
orientation: inline

TetR 1
type: SequenceAnnotation
persistent identity: annotation2400591
display id: annotation2400591
version: 1
top level: BBa_K1468004
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1468004/annotation2400591/range2400591/1

https://synbiohub.org/public/igem/BBa_K1468004/annotation2400591/range2400591/1
type: Range
persistent identity: range2400591
display id: range2400591
version: 1
direction: direction/forward
top level: BBa_K1468004
start: 1
end: 19
orientation: inline

START
type: SequenceAnnotation
persistent identity: annotation2400597
display id: annotation2400597
version: 1
top level: BBa_K1468004
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1468004/annotation2400597/range2400597/1

https://synbiohub.org/public/igem/BBa_K1468004/annotation2400597/range2400597/1
type: Range
persistent identity: range2400597
display id: range2400597
version: 1
direction: direction/forward
top level: BBa_K1468004
start: 61
end: 63
orientation: inline

LacZ
type: SequenceAnnotation
persistent identity: annotation2400596
display id: annotation2400596
version: 1
top level: BBa_K1468004
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1468004/annotation2400596/range2400596/1

https://synbiohub.org/public/igem/BBa_K1468004/annotation2400596/range2400596/1
type: Range
persistent identity: range2400596
display id: range2400596
version: 1
direction: direction/forward
top level: BBa_K1468004
start: 61
end: 3129
orientation: inline

STOP
type: SequenceAnnotation
persistent identity: annotation2400599
display id: annotation2400599
version: 1
top level: BBa_K1468004
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1468004/annotation2400599/range2400599/1

https://synbiohub.org/public/igem/BBa_K1468004/annotation2400599/range2400599/1
type: Range
persistent identity: range2400599
display id: range2400599
version: 1
direction: direction/forward
top level: BBa_K1468004
start: 3129
end: 3129
orientation: inline

BBa_I732005
type: SequenceAnnotation
persistent identity: annotation2400600
display id: annotation2400600
version: 1
top level: BBa_K1468004
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1468004/annotation2400600/range2400600/1

https://synbiohub.org/public/igem/BBa_K1468004/annotation2400600/range2400600/1
type: Range
persistent identity: range2400600
display id: range2400600
version: 1
direction: direction/forward
top level: BBa_K1468004
start: 61
end: 3129
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2400593
display id: annotation2400593
version: 1
top level: BBa_K1468004
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1468004/annotation2400593/range2400593/1

https://synbiohub.org/public/igem/BBa_K1468004/annotation2400593/range2400593/1
type: Range
persistent identity: range2400593
display id: range2400593
version: 1
direction: direction/forward
top level: BBa_K1468004
start: 1
end: 54
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2400595
display id: annotation2400595
version: 1
top level: BBa_K1468004
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1468004/annotation2400595/range2400595/1

https://synbiohub.org/public/igem/BBa_K1468004/annotation2400595/range2400595/1
type: Range
persistent identity: range2400595
display id: range2400595
version: 1
direction: direction/forward
top level: BBa_K1468004
start: 43
end: 48
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2400592
display id: annotation2400592
version: 1
top level: BBa_K1468004
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1468004/annotation2400592/range2400592/1

https://synbiohub.org/public/igem/BBa_K1468004/annotation2400592/range2400592/1
type: Range
persistent identity: range2400592
display id: range2400592
version: 1
direction: direction/forward
top level: BBa_K1468004
start: 20
end: 25
orientation: inline

BBa_J64024
type: ComponentDefinition
persistent identity: BBa J64024
display id: BBa_J64024
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: sipC RBS
creator: Dan Widmaier
created: 2008-01-16T12:00:00Z
modified: 2015-08-31T01:56:26Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _98_
m user id: 0
owner id: 2407
owning group id: 98
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This part is the natural RBS from the sipC protein in Salmonella Type III Secretion.
mutable notes: This part was not designed with biobrick standard assembly in mind. This RBS has not been explicitly measured for expression rate using fluorescence or B-Gal assay.
mutable provenance: Salmonella LT2 Genomic DNA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: sipC RBS
sequence: https://synbiohub.org/public/igem/BBa_J64024_sequence/1

sipC RBS
type: SequenceAnnotation
persistent identity: annotation1959623
display id: annotation1959623
version: 1
top level: BBa_J64024
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_J64024/annotation1959623/range1959623/1

https://synbiohub.org/public/igem/BBa_J64024/annotation1959623/range1959623/1
type: Range
persistent identity: range1959623
display id: range1959623
version: 1
direction: direction/forward
top level: BBa_J64024
start: 1
end: 15
orientation: inline

BBa_K118012
type: ComponentDefinition
persistent identity: BBa K118012
display id: BBa_K118012
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Synthetic ribosome binding site added by Son of Babel procedure
creator: Andrew Hall
created: 2008-08-18T11:00:00Z
modified: 2015-05-08T01:09:36Z
discontinued: false
dominant: false
experience: experience/None
group u list: _192_
m user id: 0
owner id: 3282
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is a 4bp synthetic ribosome binding site which can be added by fusion PCR using a reverse primer and the Son of Babel cloning system.
mutable notes: The part can be added by fusion PCR using a Son of Babel reverse primer.
mutable provenance: Synthetic DNA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_K118012_sequence/1

p(osmY)
type: ComponentDefinition
persistent identity: BBa J45993
display id: BBa_J45993
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Minimal stationary phase osmY promoter
creator: Stephen Payne
created: 2006-07-10T11:00:00Z
modified: 2015-08-31T04:08:50Z
discontinued: false
dominant: false
experience: experience/Fails
group u list: _84_
m user id: 0
owner id: 642
owning group id: 84
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: osmY's promoter is active in stationary phase and under high osmotic pressure conditions.
mutable notes: Truncated Forward Primer: 5'- GTT TCT TCG AAT TCG CGG CCG CTT CTA GGCT TAT GTT TTC GCT GAT ATC - 3' Total Length: 49 bp Annealing Length: 21 bp GC Content: 38.1% Melting Temperature: 49.5 degrees C hairpin deltaG: -2.66 kcal/mol self dimer deltaG: -99.97 kcal/mol Reverse Primer: 5'-GTT TCT TCC TGC AGC GGC CGC TAC TAG TAT TGT TAA ATA TAG ATC ACA ATT TTG- 3' Total Length: 54 bp Annealing Length: 25 bp GC Content: 20.0% Melting Temperature: 46.4 degrees C hairpin deltaG: -2.86 kcal/mol self dimer deltaG: -99.44 kcal/mol heterodimer deltaG with Conserved Forward Primer: -99.97 kcal/mol
mutable provenance: This part was PCRed out of E. coli's genome.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -10
sequence annotation: -35
sequence: https://synbiohub.org/public/igem/BBa_J45993_sequence/1

-10
type: SequenceAnnotation
persistent identity: annotation1883647
display id: annotation1883647
version: 1
top level: p(osmY)
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J45993/annotation1883647/range1883647/1

https://synbiohub.org/public/igem/BBa_J45993/annotation1883647/range1883647/1
type: Range
persistent identity: range1883647
display id: range1883647
version: 1
direction: direction/forward
top level: p(osmY)
start: 46
end: 51
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1883648
display id: annotation1883648
version: 1
top level: p(osmY)
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J45993/annotation1883648/range1883648/1

https://synbiohub.org/public/igem/BBa_J45993/annotation1883648/range1883648/1
type: Range
persistent identity: range1883648
display id: range1883648
version: 1
direction: direction/forward
top level: p(osmY)
start: 22
end: 27
orientation: inline

BBa_K124002
type: ComponentDefinition
persistent identity: BBa K124002
display id: BBa_K124002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Yeast GPD (TDH3) Promoter
creator: John Szymanski
created: 2008-06-30T11:00:00Z
modified: 2015-05-08T01:09:44Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _221_
m user id: 0
owner id: 2512
owning group id: 58
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Regulatory region spanning 680 bp upstream of the start codon of the GPD1 gene in yeast. Very strong constitutive promoter.
mutable notes: none
mutable provenance: Mumberg, Muller, and Funk. p413 pGPD http://dx.doi.org/10.1016/0378-1119(95)00037-7
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K124002_sequence/1

BBa_J56013
type: ComponentDefinition
persistent identity: BBa J56013
display id: BBa_J56013
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Rbs2 ribosome binding site
creator: Matt Eames
created: 2006-10-31T12:00:00Z
modified: 2015-08-31T03:22:59Z
discontinued: false
dominant: false
experience: experience/None
group u list: _79_98_
m user id: 0
owner id: 1132
owning group id: 98
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: ribosome binding site for transcription initiation
mutable notes: None
mutable provenance: Synthesized by DNA 2.0
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J56013_sequence/1

PgsiB
type: ComponentDefinition
persistent identity: BBa K143011
display id: BBa_K143011
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter gsiB for B. subtilis
creator: James Chappell
created: 2008-09-10T11:00:00Z
modified: 2015-05-08T01:10:23Z
discontinued: false
dominant: false
experience: experience/None
group u list: _199_
m user id: 0
owner id: 2090
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Promoter gsiB is a sigma factor B dependent promoter found in ''B.subtilis''. In ''B.subtilis'' endogenous sigma factor B is activated under mild stress. These mild stress conditions can be generally split into nutrient stress response and physical stress response. Nutrient stress response is triggered by low levels of ATP and GTP and physical stress response is triggered by exposure to blue light, salt, heat, acid or ethanol1. The promoter gsiB has been used previously as a read out for the activation of sigma factor B 2. The context with which we used the promoter gsiB, was to take blue light as an input and give '''Polymerase Per Second'''(PoPS) as an output. To do this the other potential inputs need to be carefully controlled so that only blue light activated the sigma B and gives a PoPS output. In order to get sufficient sigma B activation by blue light the light receptor YtvA, part...., needs to be over expressed in ''B.subtilis'' 3.
mutable notes: Biobrick standard was applied to the promoter gsiB sequence.
mutable provenance: The part was designed using the sequence from the ''B.subtilis'' genome and from previously published papers 23. This sequence was then synthesised by Geneart.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: Sigma B -35
sequence annotation: Sigma B -10
sequence: https://synbiohub.org/public/igem/BBa_K143011_sequence/1

Sigma B -35
type: SequenceAnnotation
persistent identity: annotation1975702
display id: annotation1975702
version: 1
top level: PgsiB
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K143011/annotation1975702/range1975702/1

https://synbiohub.org/public/igem/BBa_K143011/annotation1975702/range1975702/1
type: Range
persistent identity: range1975702
display id: range1975702
version: 1
direction: direction/forward
top level: PgsiB
start: 6
end: 11
orientation: inline

Sigma B -10
type: SequenceAnnotation
persistent identity: annotation1975703
display id: annotation1975703
version: 1
top level: PgsiB
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K143011/annotation1975703/range1975703/1

https://synbiohub.org/public/igem/BBa_K143011/annotation1975703/range1975703/1
type: Range
persistent identity: range1975703
display id: range1975703
version: 1
direction: direction/forward
top level: PgsiB
start: 25
end: 30
orientation: inline

BBa_K777105
type: ComponentDefinition
persistent identity: BBa K777105
display id: BBa_K777105
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: flhDC operon under the control of constitutive promoter J23109
creator: Team Goettingen
created: 2012-09-18T11:00:00Z
modified: 2015-05-08T01:13:16Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1029_
m user id: 0
owner id: 12106
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: The ''flhDC'' operon is the master regulator of motility and chemotaxis in ''E. coli''. FlhDC forms heterotetramers and activates class II operons in concert with sigma factor 70. Among the gene products of class II operons are several components of the flagellum and the alternative sigma factor FliA which is essential for the transcription of class III genes. Here we used a set of 8 constitutive Anderson-promoters from the 2006 Berkeley group to test how different levels of constitutive ''flhDC'' expression affect the motility of ''E. coli''.
mutable notes: *The PstI restriction site in the ''flhDC'' sequence was mutated to CTGCGG using the reverse primer. *''flhDC'' was amplified from genomic DNA of ''E. coli'' DH10B via PCR using the following primers: **Fwd: 5'-actgaattcgcggccgcttctagatgcatacctccgagttgctg-3' **Rev: 5'-tcctgcagcggccgctactagttactgcccgggatggcggttgacataagcCgcaggcaaagctgccaacag-3' (the capitalized "C" induces the mutation for removal of the PstI site)
mutable provenance: *The part was amplified from genomic DNA of ''E. coli'' str. K-12 substr. DH10B, complete genome (CP000948.1). *J23109 information was taken from the partsregistry and physical DNA from the 2012 distribution kit.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K777100
component: BBa_J23109
sequence annotation: BBa_J23109
sequence annotation: BBa_K777100
sequence: https://synbiohub.org/public/igem/BBa_K777105_sequence/1

BBa_K777100
type: Component
persistent identity: component2187056
display id: component2187056
version: 1
top level: BBa_K777105
access: public
definition: https://synbiohub.org/public/igem/BBa_K777100/1

BBa_J23109
type: Component
persistent identity: component2187048
display id: component2187048
version: 1
top level: BBa_K777105
access: public
definition: BBa_J23109

BBa_J23109
type: SequenceAnnotation
persistent identity: annotation2187048
display id: annotation2187048
version: 1
top level: BBa_K777105
location: https://synbiohub.org/public/igem/BBa_K777105/annotation2187048/range2187048/1
component: BBa_J23109

https://synbiohub.org/public/igem/BBa_K777105/annotation2187048/range2187048/1
type: Range
persistent identity: range2187048
display id: range2187048
version: 1
direction: direction/reverse
top level: BBa_K777105
start: 1
end: 35
orientation: inline

BBa_K777100
type: SequenceAnnotation
persistent identity: annotation2187056
display id: annotation2187056
version: 1
top level: BBa_K777105
location: https://synbiohub.org/public/igem/BBa_K777105/annotation2187056/range2187056/1
component: BBa_K777100

https://synbiohub.org/public/igem/BBa_K777105/annotation2187056/range2187056/1
type: Range
persistent identity: range2187056
display id: range2187056
version: 1
direction: direction/reverse
top level: BBa_K777105
start: 42
end: 910
orientation: inline

BBa_J61123
type: ComponentDefinition
persistent identity: BBa J61123
display id: BBa_J61123
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ribosome Binding Site Family Member
creator: John Anderson
created: 2007-04-24T11:00:00Z
modified: 2015-08-31T01:59:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable description: {{JCA_Arkin_RBSFamily}}
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J61123_sequence/1

BBa_B0074
type: ComponentDefinition
persistent identity: BBa B0074
display id: BBa_B0074
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Orthogonal RBS
creator: Bartholomew Canton
created: 2006-05-01T11:00:00Z
modified: 2015-08-31T04:07:21Z
discontinued: false
dominant: true
experience: experience/None
group u list: _11_6_
m user id: 0
owner id: 135
owning group id: 6
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_B0074_sequence/1

T7 0.5
type: ComponentDefinition
persistent identity: BBa B2003
display id: BBa_B2003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: T7 RBS 0.5
creator: Heather Keller
created: 2006-09-27T11:00:00Z
modified: 2015-08-31T04:07:21Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_
m user id: 0
owner id: 571
owning group id: 10
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is the -20 to -1 region of T7 gp0.5, which contains the native RBS region for this gene. The physical instantiation of this part does not exist. This part is not compatible with Standard Biobricks assembly methods, as the scar created during assembly would interrupt the spacing between the RBS and the start codon of the CDS. Individuals wishing to use this RBS sequence in their assembles should refer to part B2103.
mutable notes: The -20 to -1 region was chosen to be representative of the RBS region of the T7 genes for the following reasons: 1) The Shine-Delgarno region is contained within this defined region for all T7 genes. 2) Current literature suggests that when bound to the Translation Start Site (0), the Ribosome covers positions -20 to +13 of the gene.
mutable provenance: Genome of wild-type bacteriophage T7, positions 1476-1495. Includes the -20 to -1 region of gp0.5.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: SD region
sequence: https://synbiohub.org/public/igem/BBa_B2003_sequence/1

SD region
type: SequenceAnnotation
persistent identity: annotation1902509
display id: annotation1902509
version: 1
top level: T7 0.5
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_B2003/annotation1902509/range1902509/1

https://synbiohub.org/public/igem/BBa_B2003/annotation1902509/range1902509/1
type: Range
persistent identity: range1902509
display id: range1902509
version: 1
direction: direction/forward
top level: T7 0.5
start: 13
end: 18
orientation: inline

BBa_J64022
type: ComponentDefinition
persistent identity: BBa J64022
display id: BBa_J64022
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: sicA RBS
creator: Dan Widmaier
created: 2008-01-16T12:00:00Z
modified: 2015-08-31T01:56:26Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _98_
m user id: 0
owner id: 2407
owning group id: 98
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is the natural RBS from the sicA chaperone (BBa_J6021) in the Salmonella Type III Secretion System.
mutable notes: This part was not designed with standard biobrick assembly in mind.
mutable provenance: Salmonella LT2 Genomic DNA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: misc
sequence: https://synbiohub.org/public/igem/BBa_J64022_sequence/1

misc
type: SequenceAnnotation
persistent identity: annotation1959621
display id: annotation1959621
version: 1
top level: BBa_J64022
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J64022/annotation1959621/range1959621/1

https://synbiohub.org/public/igem/BBa_J64022/annotation1959621/range1959621/1
type: Range
persistent identity: range1959621
display id: range1959621
version: 1
direction: direction/forward
top level: BBa_J64022
start: 1
end: 20
orientation: inline

BBa_K112907
type: ComponentDefinition
persistent identity: BBa K112907
display id: BBa_K112907
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Pcon
creator: Bing Xia
created: 2008-10-23T11:00:00Z
modified: 2015-05-08T01:09:20Z
discontinued: false
dominant: false
group u list: _224_
m user id: 0
owner id: 2998
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: A constituitive promoter
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K112907_sequence/1

BBa_B0077
type: ComponentDefinition
persistent identity: BBa B0077
display id: BBa_B0077
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Specialized RBS
creator: Bartholomew Canton
created: 2006-05-01T11:00:00Z
modified: 2015-08-31T04:07:21Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_6_
m user id: 0
owner id: 135
owning group id: 6
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_B0077_sequence/1

BBa_K105029
type: ComponentDefinition
persistent identity: BBa K105029
display id: BBa_K105029
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: cyc43 minimal promoter
creator: Michael Wild
created: 2008-10-25T11:00:00Z
modified: 2015-05-08T01:08:52Z
discontinued: false
dominant: false
experience: experience/None
group u list: _253_
m user id: 0
owner id: 3313
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This is a derivate of BBa_K105027. Due to a point mutation in the TATA box region the efficiency of this minimal promoter is only 43 % of the natural yeast CYC1 promoter.
mutable notes: This BioBrick contains the TATA-box with one point mutation and most of the 5'UTR (-139 to -36, relative to the translational start) of the natural CYC1 promoter. A Kozak sequence is not included and has thus to be added! Even if there isn't any need for this BioBrick to be fusible to other protein domains, we stayed with our design.
mutable provenance: Amplification and Mutation from the plasmid pAH3
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: TATA-box
sequence annotation: A -> G
sequence: https://synbiohub.org/public/igem/BBa_K105029_sequence/1

TATA-box
type: SequenceAnnotation
persistent identity: annotation1989837
display id: annotation1989837
version: 1
top level: BBa_K105029
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K105029/annotation1989837/range1989837/1

https://synbiohub.org/public/igem/BBa_K105029/annotation1989837/range1989837/1
type: Range
persistent identity: range1989837
display id: range1989837
version: 1
direction: direction/forward
top level: BBa_K105029
start: 17
end: 23
orientation: inline

A -> G
type: SequenceAnnotation
persistent identity: annotation1989838
display id: annotation1989838
version: 1
top level: BBa_K105029
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_K105029/annotation1989838/range1989838/1

https://synbiohub.org/public/igem/BBa_K105029/annotation1989838/range1989838/1
type: Range
persistent identity: range1989838
display id: range1989838
version: 1
direction: direction/forward
top level: BBa_K105029
start: 23
end: 23
orientation: inline

BBa_K105027
type: ComponentDefinition
persistent identity: BBa K105027
display id: BBa_K105027
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: cyc100 minimal promoter
creator: Michael Wild
created: 2008-10-25T11:00:00Z
modified: 2015-05-08T01:08:52Z
discontinued: false
dominant: false
experience: experience/None
group u list: _253_
m user id: 0
owner id: 3313
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This is the core of the CYC1 promoter of ''S. cerevisiae''. This promoter element allows basal transcription. The transcription signal can be modulated by adding operator sites upstream of this BioBrick. In that way activators or repressors can act on the transcription machinery. <br<
mutable notes: This BioBrick contains the TATA-box and most of the 5'UTR (-139 to -36, relative to the translational start)of the natural CYC1 promoter. A Kozak sequence is not included and has thus to be added!
mutable provenance: Amplification from the plasmid pAH3.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: TATA box
sequence: https://synbiohub.org/public/igem/BBa_K105027_sequence/1

TATA box
type: SequenceAnnotation
persistent identity: annotation1989774
display id: annotation1989774
version: 1
top level: BBa_K105027
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K105027/annotation1989774/range1989774/1

https://synbiohub.org/public/igem/BBa_K105027/annotation1989774/range1989774/1
type: Range
persistent identity: range1989774
display id: range1989774
version: 1
direction: direction/forward
top level: BBa_K105027
start: 17
end: 23
orientation: inline

BBa_Z0253
type: ComponentDefinition
persistent identity: BBa Z0253
display id: BBa_Z0253
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: T7 weak binding promoter
creator: Sriram Kosuri
created: 2006-05-15T11:00:00Z
modified: 2015-05-08T01:14:55Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_10_
m user id: 0
owner id: 64
owning group id: 10
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: ag->GA from concensus
sequence annotation: ag->TA from concensus
sequence: https://synbiohub.org/public/igem/BBa_Z0253_sequence/1

ag->GA from concensus
type: SequenceAnnotation
persistent identity: annotation1862691
display id: annotation1862691
version: 1
top level: BBa_Z0253
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_Z0253/annotation1862691/range1862691/1

https://synbiohub.org/public/igem/BBa_Z0253/annotation1862691/range1862691/1
type: Range
persistent identity: range1862691
display id: range1862691
version: 1
direction: direction/forward
top level: BBa_Z0253
start: 31
end: 32
orientation: inline

ag->TA from concensus
type: SequenceAnnotation
persistent identity: annotation1862684
display id: annotation1862684
version: 1
top level: BBa_Z0253
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_Z0253/annotation1862684/range1862684/1

https://synbiohub.org/public/igem/BBa_Z0253/annotation1862684/range1862684/1
type: Range
persistent identity: range1862684
display id: range1862684
version: 1
direction: direction/forward
top level: BBa_Z0253
start: 27
end: 28
orientation: inline

BBa_K777001
type: ComponentDefinition
persistent identity: BBa K777001
display id: BBa_K777001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Tar receptor under the control of constitutive promoter J23100
creator: Bianca Genenncher
created: 2012-09-20T11:00:00Z
modified: 2015-05-08T01:13:16Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1029_
m user id: 0
owner id: 11946
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: The transmembrane chemoreceptor Tar of E. coli mediates chemotaxis towards aspartate. Here we used a set of 8 constitutive [http://partsregistry.org/Part:BBa_J23100 Anderson promoters] from the 2006 Berkeley group to test how different levels of constitutive ''flhDC'' expression affect the motility of ''E. coli''.
mutable notes: * Genomic sequence was amplified using the following primers. ** Primers PCR: Base pairs in caps represent the respective restriction sites followed by either prefix or suffix. Primers were provided by SIGMA. *** Fwd: Tar for + prefix: 5?? actGAATTCgcggccgctTCTAGAtgattaaccgtatccgcgtagtc 3?? *** Rev: Tar rev + suffix: 5?? tcCTGCAGcggccgctACTAGTcaaaatgtttcccagtttggatc 3?? * One XbaI site was found at position 420. The restriction site was changed from TCTAGt to CCTAGt via QuikChange PCR. ** Primers QuikChange (QC): Primers were provided by SIGMA. *** Fwd: TarQC for: 5?? ACTGATTGATTAcCTAGATTATGGCAATACTGGAG 3?? *** Rev: TarQC rev: 5?? TGCCATAATCTAGgTAATCAATCAGTTCAGTTAAC 3?? (the uncapitalized letter induces the mutation for removal of the XbaI site)
mutable provenance: *The part was amplified from genomic DNA of ''E. coli'' str. K-12 substr. DH10B, complete genome (CP000948.1). *J23100 information was taken from the partsregistry and physical DNA from the 2012 distribution kit.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K777000
component: BBa_J23100
sequence annotation: BBa_K777000
sequence annotation: BBa_J23100
sequence: https://synbiohub.org/public/igem/BBa_K777001_sequence/1

BBa_K777000
type: Component
persistent identity: component2189126
display id: component2189126
version: 1
top level: BBa_K777001
access: public
definition: BBa_K777000

BBa_J23100
type: Component
persistent identity: component2189122
display id: component2189122
version: 1
top level: BBa_K777001
access: public
definition: BBa_J23100

BBa_K777000
type: SequenceAnnotation
persistent identity: annotation2189126
display id: annotation2189126
version: 1
top level: BBa_K777001
location: https://synbiohub.org/public/igem/BBa_K777001/annotation2189126/range2189126/1
component: BBa_K777000

https://synbiohub.org/public/igem/BBa_K777001/annotation2189126/range2189126/1
type: Range
persistent identity: range2189126
display id: range2189126
version: 1
direction: direction/reverse
top level: BBa_K777001
start: 42
end: 1703
orientation: inline

BBa_J23100
type: SequenceAnnotation
persistent identity: annotation2189122
display id: annotation2189122
version: 1
top level: BBa_K777001
location: https://synbiohub.org/public/igem/BBa_K777001/annotation2189122/range2189122/1
component: BBa_J23100

https://synbiohub.org/public/igem/BBa_K777001/annotation2189122/range2189122/1
type: Range
persistent identity: range2189122
display id: range2189122
version: 1
direction: direction/reverse
top level: BBa_K777001
start: 1
end: 35
orientation: inline

T7 RBS
type: ComponentDefinition
persistent identity: BBa K1362090
display id: BBa_K1362090
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: strong T7 RBS
creator: Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena B??scher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Sch??fer, Carolin Schmelas, Silvan Schmitz, Max Waldha
created: 2014-10-02T11:00:00Z
modified: 2015-05-08T01:10:04Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1738_
m user id: 0
owner id: 22830
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: RFC10 compatible strong RBS derived from the T7 phage gene 10a (major capsid protein)[1]. When assembled to a coding part with the A of the start codon being part of the XbaI site, the RBS will be shifted one bp downstream compared to the native sequence. The sequence was successfully used by the iGEM team Heidelberg 2014 for the expression of many proteins in E.coli. 1. Olinss, P. & Rangwala, S. H. Derived from Bacteriophage T7 mRNA Acts ELS an Enhancer of Translation of the lac2 Gene in. 16973???16976 (1989).
mutable notes: The 18 bp including the XbaI that can be found upstream of the presumably important part of the RBS were included into the sequence just to make sure it works. However to fully comply with RFC10 a G was inserted behind the XbaI site.
mutable provenance: synthesized as found in the T7 genome and several commercial expression plasmids.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: Shine-Dalgarno
sequence annotation: t7 RBS
sequence: https://synbiohub.org/public/igem/BBa_K1362090_sequence/1

Shine-Dalgarno
type: SequenceAnnotation
persistent identity: annotation2393796
display id: annotation2393796
version: 1
top level: T7 RBS
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1362090/annotation2393796/range2393796/1

https://synbiohub.org/public/igem/BBa_K1362090/annotation2393796/range2393796/1
type: Range
persistent identity: range2393796
display id: range2393796
version: 1
direction: direction/forward
top level: T7 RBS
start: 21
end: 28
orientation: inline

t7 RBS
type: SequenceAnnotation
persistent identity: annotation2393795
display id: annotation2393795
version: 1
top level: T7 RBS
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1362090/annotation2393795/range2393795/1

https://synbiohub.org/public/igem/BBa_K1362090/annotation2393795/range2393795/1
type: Range
persistent identity: range2393795
display id: range2393795
version: 1
direction: direction/forward
top level: T7 RBS
start: 11
end: 28
orientation: inline

BBa_K1585118
type: ComponentDefinition
persistent identity: BBa K1585118
display id: BBa_K1585118
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Anderson Promoter with lacI binding site
creator: Tobias Schwanemann
created: 2015-08-31T11:00:00Z
modified: 2015-09-01T02:31:42Z
discontinued: false
dominant: false
experience: experience/Fails
group u list: _2002_
m user id: 24474
owner id: 24474
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Promoters of the Anderson Promoter Collection with lacI binding site derived from [http://parts.igem.org/Part:BBa_R0010 R_0010] to make them [http://openwetware.org/wiki/IPTG IPTG] iducible.
mutable notes: We choosed lacI-binding-site of part BBa_R0010 because we assumed older parts would be more reliable. The part is shipped in backbone [http://parts.igem.org/Part:BBa_J61002 J61002] because the functionality is directly visible by adding IPTG. RFP ([http://parts.igem.org/Part:BBa_E1010 E1010]) should get expressed then.
mutable provenance: Assambly of [http://parts.igem.org/Part:BBa_J23118 J23118] and lacI-binding-site of [http://parts.igem.org/Part:BBa_R0010 R_0010] The "lacI binding site" of Part [http://parts.igem.org/Part:BBa_R0010 R_0010] differs from lacO from Part [http://parts.igem.org/Part:BBa_J33207 BBa_J33207].
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
component: BBa_J23118
component: BBa_K1585999
sequence annotation: BBa_K1585999
sequence annotation: BBa_J23118
sequence: https://synbiohub.org/public/igem/BBa_K1585118_sequence/1

BBa_J23118
type: Component
persistent identity: component2441715
display id: component2441715
version: 1
top level: BBa_K1585118
access: public
definition: BBa_J23118

BBa_K1585999
type: Component
persistent identity: component2441717
display id: component2441717
version: 1
top level: BBa_K1585118
access: public
definition: https://synbiohub.org/public/igem/BBa_K1585999/1

BBa_K1585999
type: SequenceAnnotation
persistent identity: annotation2441717
display id: annotation2441717
version: 1
top level: BBa_K1585118
location: https://synbiohub.org/public/igem/BBa_K1585118/annotation2441717/range2441717/1
component: BBa_K1585999

https://synbiohub.org/public/igem/BBa_K1585118/annotation2441717/range2441717/1
type: Range
persistent identity: range2441717
display id: range2441717
version: 1
direction: direction/reverse
top level: BBa_K1585118
start: 44
end: 78
orientation: inline

BBa_J23118
type: SequenceAnnotation
persistent identity: annotation2441715
display id: annotation2441715
version: 1
top level: BBa_K1585118
location: https://synbiohub.org/public/igem/BBa_K1585118/annotation2441715/range2441715/1
component: BBa_J23118

https://synbiohub.org/public/igem/BBa_K1585118/annotation2441715/range2441715/1
type: Range
persistent identity: range2441715
display id: range2441715
version: 1
direction: direction/reverse
top level: BBa_K1585118
start: 1
end: 35
orientation: inline

BBa_K777101
type: ComponentDefinition
persistent identity: BBa K777101
display id: BBa_K777101
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: flhDC operon under the control of constitutive promoter J23100
creator: Team Goettingen
created: 2012-09-18T11:00:00Z
modified: 2015-05-08T01:13:16Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1029_
m user id: 0
owner id: 12106
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: The flhDC operon is the master regulator of motility and chemotaxis in E. coli. FlhDC forms heterotetramers and activates class II operons in concert with sigma factor 70. Among the gene products of class II operons are several components of the flagellum and the alternative sigma factor FliA which is essential for the transcription of class III genes. Here we used a set of 8 constitutive promoters from the 2006 Berkeley group to test how different levels of constitutive ''flhDC'' expression affect motility in ''E. coli''.
mutable notes: *The PstI restriction site in the ''flhDC'' sequence was mutated to CTGCGG using the reverse primer. *''flhDC'' was amplified from genomic DNA of ''E. coli'' DH10B via PCR using the following primers: **Fwd: 5'-actgaattcgcggccgcttctagatgcatacctccgagttgctg-3' **Rev: 5'-tcctgcagcggccgctactagttactgcccgggatggcggttgacataagcCgcaggcaaagctgccaacag-3' (the capitalized "C" induces the mutation for removal of the PstI site)
mutable provenance: *The part was amplified from genomic DNA of ''E. coli'' str. K-12 substr. DH10B, complete genome (CP000948.1). *J23100 information was from partsregistry and physical DNA from the 2012 distribution kit.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K777100
component: BBa_J23100
sequence annotation: BBa_J23100
sequence annotation: BBa_K777100
sequence: https://synbiohub.org/public/igem/BBa_K777101_sequence/1

BBa_K777100
type: Component
persistent identity: component2186537
display id: component2186537
version: 1
top level: BBa_K777101
access: public
definition: https://synbiohub.org/public/igem/BBa_K777100/1

BBa_J23100
type: Component
persistent identity: component2186529
display id: component2186529
version: 1
top level: BBa_K777101
access: public
definition: BBa_J23100

BBa_J23100
type: SequenceAnnotation
persistent identity: annotation2186529
display id: annotation2186529
version: 1
top level: BBa_K777101
location: https://synbiohub.org/public/igem/BBa_K777101/annotation2186529/range2186529/1
component: BBa_J23100

https://synbiohub.org/public/igem/BBa_K777101/annotation2186529/range2186529/1
type: Range
persistent identity: range2186529
display id: range2186529
version: 1
direction: direction/reverse
top level: BBa_K777101
start: 1
end: 35
orientation: inline

BBa_K777100
type: SequenceAnnotation
persistent identity: annotation2186537
display id: annotation2186537
version: 1
top level: BBa_K777101
location: https://synbiohub.org/public/igem/BBa_K777101/annotation2186537/range2186537/1
component: BBa_K777100

https://synbiohub.org/public/igem/BBa_K777101/annotation2186537/range2186537/1
type: Range
persistent identity: range2186537
display id: range2186537
version: 1
direction: direction/reverse
top level: BBa_K777101
start: 42
end: 910
orientation: inline

BBa_K112706
type: ComponentDefinition
persistent identity: BBa K112706
display id: BBa_K112706
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Pspv2 from Salmonella
creator: Bing Xia
created: 2008-10-20T11:00:00Z
modified: 2015-05-08T01:09:18Z
discontinued: false
dominant: false
experience: experience/None
group u list: _224_
m user id: 0
owner id: 2998
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: The spv2 promoter. ---- This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at: [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
mutable provenance: Construction of <Pspv2> basic part K112706 PCR of dv015/dv016 on pBca1037 (500bp, EcoRI/BamHI/DpnI) Sub into pBca1256 (EcoRI/BamHI, 2472+ 500, L) Product is pBca1256-K112706 ----------------------------------------------- dv015 Forward biobricking of <Pspv2> cgataGAATTCatgAGATCTccttatgcagcgtaagggccgcaac dv016 Reverse biobricking of <Pspv2> cgttaGGATCCGATAATGTtTGCAGGGGAATTATTTTG
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K112706_sequence/1

BBa_B0032
type: ComponentDefinition
persistent identity: BBa B0032
display id: BBa_B0032
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS.3 (medium) -- derivative of BBa_0030
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _41_44_48_46_1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Weak1 RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0033</bb_part>.
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("RBS-2" in figure 4-14 of thesis). Contact info for this part: Brian Chow
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: RBS-3\Weak
sequence annotation: BBa_B0032
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_B0032_sequence/1

RBS-3\Weak
type: SequenceAnnotation
persistent identity: annotation1709
display id: annotation1709
version: 1
top level: BBa_B0032
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_B0032/annotation1709/range1709/1

https://synbiohub.org/public/igem/BBa_B0032/annotation1709/range1709/1
type: Range
persistent identity: range1709
display id: range1709
version: 1
direction: direction/forward
top level: BBa_B0032
start: 1
end: 13
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation7027
display id: annotation7027
version: 1
top level: BBa_B0032
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_B0032/annotation7027/range7027/1

https://synbiohub.org/public/igem/BBa_B0032/annotation7027/range7027/1
type: Range
persistent identity: range7027
display id: range7027
version: 1
direction: direction/forward
top level: BBa_B0032
start: 1
end: 13
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1710
display id: annotation1710
version: 1
top level: BBa_B0032
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_B0032/annotation1710/range1710/1

https://synbiohub.org/public/igem/BBa_B0032/annotation1710/range1710/1
type: Range
persistent identity: range1710
display id: range1710
version: 1
direction: direction/forward
top level: BBa_B0032
start: 7
end: 10
orientation: inline

BBa_M13508
type: ComponentDefinition
persistent identity: BBa M13508
display id: BBa_M13508
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: M13K07 gene VIII RBS
creator: Natalie Kuldell
created: 2006-12-14T12:00:00Z
modified: 2015-05-08T01:13:56Z
discontinued: false
dominant: true
experience: experience/None
group u list: _45_
m user id: 0
owner id: 314
owning group id: 1
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This RBS is part of the bacteriophage M13 genome, initiating translation of the gene VIII message (BBa_M13008). The part aligns with base pairs 1285-1300 in M13K07 genome. It was identified as the RBS for gene VIII message by Wezenbeek et al in Gene (1980) 11: 129-148
mutable notes: none
mutable provenance: New England Biolabs M13K07. Sequenced in 2002 by L. Panganaban
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_M13508_sequence/1

BBa_K137090
type: ComponentDefinition
persistent identity: BBa K137090
display id: BBa_K137090
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: optimized (A) repeat constitutive promoter with 17 bp between -10 and -35 elements
creator: Allen Lin
created: 2008-08-20T11:00:00Z
modified: 2015-05-08T01:10:10Z
discontinued: false
dominant: false
experience: experience/None
group u list: _187_
m user id: 0
owner id: 3112
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: optimized (A) repeat constitutive promoter with 17 bp between -10 and -35 elements
mutable notes: None
mutable provenance: PCR
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K137090_sequence/1

BBa_J61138
type: ComponentDefinition
persistent identity: BBa J61138
display id: BBa_J61138
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ribosome Binding Site Family Member
creator: John Anderson
created: 2007-04-24T11:00:00Z
modified: 2015-08-31T01:59:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable description: {{JCA_Arkin_RBSFamily}}
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J61138_sequence/1

BBa_M13501
type: ComponentDefinition
persistent identity: BBa M13501
display id: BBa_M13501
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: M13K07 gene I RBS
creator: Natalie Kuldell
created: 2006-12-14T12:00:00Z
modified: 2015-05-08T01:13:56Z
discontinued: false
dominant: false
experience: experience/None
group u list: _45_
m user id: 0
owner id: 314
owning group id: 1
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This RBS is part of the bacteriophage M13 genome, initiating translation of the gene I message (BBa_M13001). The part aligns with base pairs 8252-8267 in M13K07 genome. It was identified as the RBS for gene II message by Wezenbeek et al in Gene (1980) 11: 129-148
mutable notes: none
mutable provenance: New England Biolabs M13K07. Sequenced in 2002 by L. Panganaban
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_M13501_sequence/1

P43
type: ComponentDefinition
persistent identity: BBa K143013
display id: BBa_K143013
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter 43 a constitutive promoter for B. subtilis
creator: James Chappell
created: 2008-09-10T11:00:00Z
modified: 2015-05-08T01:10:23Z
discontinued: false
dominant: false
experience: experience/None
group u list: _199_
m user id: 0
owner id: 2090
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Promoter 43 is a constitutive promoter that constitutively expresses the P43 protein in ''B.subtilis''. This promoter has been shown to be recognized and active during the exponential and lag phases of growth. It has been hypothesized that the ability to recognize the promoter in exponential and lag phase of growth is due to the recognition of the promoter by both sigma factor 55 (the major sigma factor) and sigma factor 37 (the lag phase sigma factor) 1. The P43 promoter has been previously used for constitutive expression of exogenous genes within ''B.subtilis'' vectors 2. The context with which we used the promoter P43 is as a '''Polymerase Per Second''' (PoPS) generator.
mutable notes: The biobrick part was designed to include the binding sites for both the sigma factor A and B. In addition the biobrick standard was applied to the promoter P43 sequence.
mutable provenance: The part was designed using the sequence from the ''B.subtilis'' genome and from previously published papers 2. This sequence was then synthesised by Geneart.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: Sigma B -10
sequence annotation: Sigma A -35
sequence annotation: Sigma A -10
sequence annotation: Sigma B -35
sequence: https://synbiohub.org/public/igem/BBa_K143013_sequence/1

Sigma B -10
type: SequenceAnnotation
persistent identity: annotation1975707
display id: annotation1975707
version: 1
top level: P43
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K143013/annotation1975707/range1975707/1

https://synbiohub.org/public/igem/BBa_K143013/annotation1975707/range1975707/1
type: Range
persistent identity: range1975707
display id: range1975707
version: 1
direction: direction/forward
top level: P43
start: 38
end: 47
orientation: inline

Sigma A -35
type: SequenceAnnotation
persistent identity: annotation1975708
display id: annotation1975708
version: 1
top level: P43
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K143013/annotation1975708/range1975708/1

https://synbiohub.org/public/igem/BBa_K143013/annotation1975708/range1975708/1
type: Range
persistent identity: range1975708
display id: range1975708
version: 1
direction: direction/forward
top level: P43
start: 24
end: 29
orientation: inline

Sigma A -10
type: SequenceAnnotation
persistent identity: annotation1975709
display id: annotation1975709
version: 1
top level: P43
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K143013/annotation1975709/range1975709/1

https://synbiohub.org/public/igem/BBa_K143013/annotation1975709/range1975709/1
type: Range
persistent identity: range1975709
display id: range1975709
version: 1
direction: direction/forward
top level: P43
start: 47
end: 52
orientation: inline

Sigma B -35
type: SequenceAnnotation
persistent identity: annotation1975706
display id: annotation1975706
version: 1
top level: P43
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K143013/annotation1975706/range1975706/1

https://synbiohub.org/public/igem/BBa_K143013/annotation1975706/range1975706/1
type: Range
persistent identity: range1975706
display id: range1975706
version: 1
direction: direction/forward
top level: P43
start: 14
end: 22
orientation: inline

BBa_J61114
type: ComponentDefinition
persistent identity: BBa J61114
display id: BBa_J61114
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ribosome Binding Site Family Member
creator: John Anderson
created: 2007-04-23T11:00:00Z
modified: 2015-08-31T02:03:00Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable description: {{JCA_Arkin_RBSFamily}}
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J61114_sequence/1

Cat
type: ComponentDefinition
persistent identity: BBa I14033
display id: BBa_I14033
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: P(Cat)
creator: Vikram Vijayan, Allen Hsu, Lawrence Fomundam
created: 2004-08-03T11:00:00Z
modified: 2015-08-31T04:07:37Z
discontinued: false
dominant: false
experience: experience/None
group u list: _4_
m user id: 0
owner id: 171
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Constitutive Promoter, Medium Transcription
mutable provenance: Plasmid pACYC184
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -10
sequence annotation: -35
sequence annotation: P(Cat)
sequence: https://synbiohub.org/public/igem/BBa_I14033_sequence/1

-10
type: SequenceAnnotation
persistent identity: annotation1026173
display id: annotation1026173
version: 1
top level: Cat
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I14033/annotation1026173/range1026173/1

https://synbiohub.org/public/igem/BBa_I14033/annotation1026173/range1026173/1
type: Range
persistent identity: range1026173
display id: range1026173
version: 1
direction: direction/forward
top level: Cat
start: 27
end: 32
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1026172
display id: annotation1026172
version: 1
top level: Cat
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I14033/annotation1026172/range1026172/1

https://synbiohub.org/public/igem/BBa_I14033/annotation1026172/range1026172/1
type: Range
persistent identity: range1026172
display id: range1026172
version: 1
direction: direction/forward
top level: Cat
start: 4
end: 9
orientation: inline

P(Cat)
type: SequenceAnnotation
persistent identity: annotation1026174
display id: annotation1026174
version: 1
top level: Cat
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_I14033/annotation1026174/range1026174/1

https://synbiohub.org/public/igem/BBa_I14033/annotation1026174/range1026174/1
type: Range
persistent identity: range1026174
display id: range1026174
version: 1
direction: direction/forward
top level: Cat
start: 1
end: 38
orientation: inline

BBa_K1585116
type: ComponentDefinition
persistent identity: BBa K1585116
display id: BBa_K1585116
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Anderson Promoter with lacI binding site
creator: Tobias Schwanemann
created: 2015-08-31T11:00:00Z
modified: 2015-09-01T02:19:47Z
discontinued: false
dominant: false
experience: experience/Fails
group u list: _2002_
m user id: 24474
owner id: 24474
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Promoters of the Anderson Promoter Collection with lacI binding site derived from [http://parts.igem.org/Part:BBa_R0010 R_0010] to make them [http://openwetware.org/wiki/IPTG IPTG] iducible.
mutable notes: We choosed lacI-binding-site of part BBa_R0010 because we assumed older parts would be more reliable. The part is shipped in backbone [http://parts.igem.org/Part:BBa_J61002 J61002] because the functionality is directly visible by adding IPTG. RFP ([http://parts.igem.org/Part:BBa_E1010 E1010]) should get expressed then.
mutable provenance: Assambly of [http://parts.igem.org/Part:BBa_J23103 J23103] and lacI-binding-site of [http://parts.igem.org/Part:BBa_R0010 R_0010] The "lacI binding site" of Part [http://parts.igem.org/Part:BBa_R0010 R_0010] differs from lacO from Part [http://parts.igem.org/Part:BBa_J33207 BBa_J33207].
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
component: BBa_K1585999
component: BBa_J23116
sequence annotation: BBa_K1585999
sequence annotation: BBa_J23116
sequence: https://synbiohub.org/public/igem/BBa_K1585116_sequence/1

BBa_K1585999
type: Component
persistent identity: component2441710
display id: component2441710
version: 1
top level: BBa_K1585116
access: public
definition: https://synbiohub.org/public/igem/BBa_K1585999/1

BBa_J23116
type: Component
persistent identity: component2441708
display id: component2441708
version: 1
top level: BBa_K1585116
access: public
definition: BBa_J23116

BBa_K1585999
type: SequenceAnnotation
persistent identity: annotation2441710
display id: annotation2441710
version: 1
top level: BBa_K1585116
location: https://synbiohub.org/public/igem/BBa_K1585116/annotation2441710/range2441710/1
component: BBa_K1585999

https://synbiohub.org/public/igem/BBa_K1585116/annotation2441710/range2441710/1
type: Range
persistent identity: range2441710
display id: range2441710
version: 1
direction: direction/reverse
top level: BBa_K1585116
start: 44
end: 78
orientation: inline

BBa_J23116
type: SequenceAnnotation
persistent identity: annotation2441708
display id: annotation2441708
version: 1
top level: BBa_K1585116
location: https://synbiohub.org/public/igem/BBa_K1585116/annotation2441708/range2441708/1
component: BBa_J23116

https://synbiohub.org/public/igem/BBa_K1585116/annotation2441708/range2441708/1
type: Range
persistent identity: range2441708
display id: range2441708
version: 1
direction: direction/reverse
top level: BBa_K1585116
start: 1
end: 35
orientation: inline

BBa_K165002
type: ComponentDefinition
persistent identity: BBa K165002
display id: BBa_K165002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Kozak sequence (yeast RBS)
creator: John Szymanski
created: 2008-10-25T11:00:00Z
modified: 2015-05-08T01:10:55Z
discontinued: false
dominant: false
experience: experience/None
group u list: _267_
m user id: 0
owner id: 2512
owning group id: 58
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: The kozak sequence acts as a eukaryotic RBS. It is cloned directly between a promoter and coding region.
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_K165002_sequence/1

BBa_I766556
type: ComponentDefinition
persistent identity: BBa I766556
display id: BBa_I766556
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pAdh (Strong) Promoter
creator: Nili Sommovilla
created: 2007-08-15T11:00:00Z
modified: 2015-08-31T04:08:10Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _155_
m user id: 0
owner id: 1931
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Strong Expression level Constitutive promoter in yeast
mutable notes: none
mutable provenance: Endogenous promoter for Alcohol Dehydrogenase
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I766556_sequence/1

BBa_K137030
type: ComponentDefinition
persistent identity: BBa K137030
display id: BBa_K137030
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: constitutive promoter with (TA)9 between -10 and -35 elements
creator: Allen Lin
created: 2008-07-20T11:00:00Z
modified: 2015-05-08T01:10:09Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _187_
m user id: 0
owner id: 3112
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: constitutive promoter with (TA)9 between -10 and -35 elements
mutable notes: With 9 TA repeats between the -10 and -35 elements, the distance between the two elements is not optimal for the sigma factor to bind to the promoter. Thus, the promoter should be in the 'off' state.
mutable provenance: This part was PCR amplified from two synthesized primers that bound to each other.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -10 element
sequence annotation: TA repeat
sequence: https://synbiohub.org/public/igem/BBa_K137030_sequence/1

-10 element
type: SequenceAnnotation
persistent identity: annotation1967766
display id: annotation1967766
version: 1
top level: BBa_K137030
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K137030/annotation1967766/range1967766/1

https://synbiohub.org/public/igem/BBa_K137030/annotation1967766/range1967766/1
type: Range
persistent identity: range1967766
display id: range1967766
version: 1
direction: direction/forward
top level: BBa_K137030
start: 21
end: 25
orientation: inline

TA repeat
type: SequenceAnnotation
persistent identity: annotation1967767
display id: annotation1967767
version: 1
top level: BBa_K137030
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K137030/annotation1967767/range1967767/1

https://synbiohub.org/public/igem/BBa_K137030/annotation1967767/range1967767/1
type: Range
persistent identity: range1967767
display id: range1967767
version: 1
direction: direction/forward
top level: BBa_K137030
start: 7
end: 20
orientation: inline

BBa_J61107
type: ComponentDefinition
persistent identity: BBa J61107
display id: BBa_J61107
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ribosome Binding Site Family Member
creator: John Anderson
created: 2007-04-23T11:00:00Z
modified: 2015-08-31T02:03:00Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable description: {{JCA_Arkin_RBSFamily}}
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J61107_sequence/1

BBa_K137031
type: ComponentDefinition
persistent identity: BBa K137031
display id: BBa_K137031
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: constitutive promoter with (C)10 between -10 and -35 elements
creator: Allen Lin
created: 2008-07-20T11:00:00Z
modified: 2015-05-08T01:10:09Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _187_
m user id: 0
owner id: 3112
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: constitutive promoter with (C)10 between -10 and -35 elements
mutable notes: With 10 C repeats between the -10 and -35 elements, the distance between the two elements is optimal for the sigma factor to bind to the promoter. Thus, the promoter should be in the 'on' state.
mutable provenance: This part was PCR amplified from two synthesized primers that bound to each other.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: C repeat
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_K137031_sequence/1

C repeat
type: SequenceAnnotation
persistent identity: annotation1967769
display id: annotation1967769
version: 1
top level: BBa_K137031
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K137031/annotation1967769/range1967769/1

https://synbiohub.org/public/igem/BBa_K137031/annotation1967769/range1967769/1
type: Range
persistent identity: range1967769
display id: range1967769
version: 1
direction: direction/forward
top level: BBa_K137031
start: 27
end: 36
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1967768
display id: annotation1967768
version: 1
top level: BBa_K137031
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K137031/annotation1967768/range1967768/1

https://synbiohub.org/public/igem/BBa_K137031/annotation1967768/range1967768/1
type: Range
persistent identity: range1967768
display id: range1967768
version: 1
direction: direction/forward
top level: BBa_K137031
start: 50
end: 55
orientation: inline

BBa_K1585110
type: ComponentDefinition
persistent identity: BBa K1585110
display id: BBa_K1585110
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Anderson Promoter with lacI binding site
creator: Tobias Schwanemann
created: 2015-08-31T11:00:00Z
modified: 2015-09-01T02:09:56Z
discontinued: false
dominant: false
experience: experience/Fails
group u list: _2002_
m user id: 24474
owner id: 24474
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Promoters of the Anderson Promoter Collection with lacI binding site derived from [http://parts.igem.org/Part:BBa_R0010 R_0010] to make them [http://openwetware.org/wiki/IPTG IPTG] iducible.
mutable notes: We choosed lacI-binding-site of part BBa_R0010 because we assumed older parts would be more reliable. The part is shipped in backbone [http://parts.igem.org/Part:BBa_J61002 J61002] because the functionality is directly visible by adding IPTG. RFP ([http://parts.igem.org/Part:BBa_E1010 E1010]) should get expressed then.
mutable provenance: Assambly of [http://parts.igem.org/Part:BBa_J23106 J23106] and lacI-binding-site of [http://parts.igem.org/Part:BBa_R0010 R_0010] The "lacI binding site" of Part [http://parts.igem.org/Part:BBa_R0010 R_0010] differs from lacO from Part [http://parts.igem.org/Part:BBa_J33207 BBa_J33207].
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
component: BBa_K1585999
component: BBa_J23110
sequence annotation: BBa_J23110
sequence annotation: BBa_K1585999
sequence: https://synbiohub.org/public/igem/BBa_K1585110_sequence/1

BBa_K1585999
type: Component
persistent identity: component2441701
display id: component2441701
version: 1
top level: BBa_K1585110
access: public
definition: https://synbiohub.org/public/igem/BBa_K1585999/1

BBa_J23110
type: Component
persistent identity: component2441699
display id: component2441699
version: 1
top level: BBa_K1585110
access: public
definition: BBa_J23110

BBa_J23110
type: SequenceAnnotation
persistent identity: annotation2441699
display id: annotation2441699
version: 1
top level: BBa_K1585110
location: https://synbiohub.org/public/igem/BBa_K1585110/annotation2441699/range2441699/1
component: BBa_J23110

https://synbiohub.org/public/igem/BBa_K1585110/annotation2441699/range2441699/1
type: Range
persistent identity: range2441699
display id: range2441699
version: 1
direction: direction/reverse
top level: BBa_K1585110
start: 1
end: 35
orientation: inline

BBa_K1585999
type: SequenceAnnotation
persistent identity: annotation2441701
display id: annotation2441701
version: 1
top level: BBa_K1585110
location: https://synbiohub.org/public/igem/BBa_K1585110/annotation2441701/range2441701/1
component: BBa_K1585999

https://synbiohub.org/public/igem/BBa_K1585110/annotation2441701/range2441701/1
type: Range
persistent identity: range2441701
display id: range2441701
version: 1
direction: direction/reverse
top level: BBa_K1585110
start: 44
end: 78
orientation: inline

BBa_K137032
type: ComponentDefinition
persistent identity: BBa K137032
display id: BBa_K137032
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: constitutive promoter with (C)12 between -10 and -35 elements
creator: Allen Lin
created: 2008-07-20T11:00:00Z
modified: 2015-05-08T01:10:09Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _187_
m user id: 0
owner id: 3112
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: constitutive promoter with (C)12 between -10 and -35 elements
mutable notes: With 12 C repeats between the -10 and -35 elements, the distance between the two elements is optimal for the sigma factor to bind to the promoter. Thus, the promoter should be in the 'on' state.
mutable provenance: This part was PCR amplified from two synthesized primers that bound to each other.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: c repeat
sequence annotation: -10 element
sequence: https://synbiohub.org/public/igem/BBa_K137032_sequence/1

c repeat
type: SequenceAnnotation
persistent identity: annotation1967774
display id: annotation1967774
version: 1
top level: BBa_K137032
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K137032/annotation1967774/range1967774/1

https://synbiohub.org/public/igem/BBa_K137032/annotation1967774/range1967774/1
type: Range
persistent identity: range1967774
display id: range1967774
version: 1
direction: direction/forward
top level: BBa_K137032
start: 27
end: 38
orientation: inline

-10 element
type: SequenceAnnotation
persistent identity: annotation1967773
display id: annotation1967773
version: 1
top level: BBa_K137032
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K137032/annotation1967773/range1967773/1

https://synbiohub.org/public/igem/BBa_K137032/annotation1967773/range1967773/1
type: Range
persistent identity: range1967773
display id: range1967773
version: 1
direction: direction/forward
top level: BBa_K137032
start: 52
end: 57
orientation: inline

BBa_K113010
type: ComponentDefinition
persistent identity: BBa K113010
display id: BBa_K113010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: overlapping T7 promoter
creator: Wu Jingjing, Wang Jinyu
created: 2008-10-25T11:00:00Z
modified: 2015-05-08T01:09:20Z
discontinued: false
dominant: false
experience: experience/None
group u list: _254_
m user id: 0
owner id: 2711
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Two T7 promoters are reversely overlapping.
mutable notes: It is difficult to make this short DNA by PCR as both sides are T7 promoters, which are very similar. It is highly possible that they form loops before they can be synthesized by PCR. Thus we order two single stranded DNA and then anneal them together by touchdown PCR.
mutable provenance: The sequence of T7 promoter is TAATACGACTCACTATA.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: T7 promoter
sequence: https://synbiohub.org/public/igem/BBa_K113010_sequence/1

T7 promoter
type: SequenceAnnotation
persistent identity: annotation1989307
display id: annotation1989307
version: 1
top level: BBa_K113010
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K113010/annotation1989307/range1989307/1

https://synbiohub.org/public/igem/BBa_K113010/annotation1989307/range1989307/1
type: Range
persistent identity: range1989307
display id: range1989307
version: 1
direction: direction/forward
top level: BBa_K113010
start: 20
end: 37
orientation: inline

T7 promoter
type: SequenceAnnotation
persistent identity: annotation1989277
display id: annotation1989277
version: 1
top level: BBa_K113010
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K113010/annotation1989277/range1989277/1

https://synbiohub.org/public/igem/BBa_K113010/annotation1989277/range1989277/1
type: Range
persistent identity: range1989277
display id: range1989277
version: 1
direction: direction/reverse
top level: BBa_K113010
start: 4
end: 21
orientation: inline

BBa_K292001
type: ComponentDefinition
persistent identity: BBa K292001
display id: BBa_K292001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Double terminator + Constitutive promoter + Strong RBS
creator: David Charoy
created: 2009-10-17T11:00:00Z
modified: 2015-05-08T01:11:49Z
discontinued: false
dominant: false
group u list: _394_
m user id: 0
owner id: 5212
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part contains a double terminator and a constitutive prokaryotic promoter and a strong RBS (BBa_B0014 + BBa_J23100 + BBa_B0030). It is a simple promoter in the goals to begin gene transcription. For Sup???biotech-Paris (2009) team this part is used when adding to a recombinant lambda phage and to begin the transcription of synthetic genes inside the Lambda phage genome.
mutable notes: N/A
mutable provenance: BBa_B0014 : Designed by Reshma Shetty Group: Registry (2003-07-16). BBa_J23100 : Designed by John Anderson Group: iGEM2006_Berkeley (2006-08-04.) BBa_B0030 : Designed by Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. Group: NCBS, Prather Lab.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_J23100
component: BBa_B0011
component: BBa_B0030
component: BBa_B0012
sequence annotation: BBa_B0030
sequence annotation: BBa_B0011
sequence annotation: BBa_J23100
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_K292001_sequence/1

BBa_J23100
type: Component
persistent identity: component2052039
display id: component2052039
version: 1
top level: BBa_K292001
access: public
definition: BBa_J23100

BBa_B0011
type: Component
persistent identity: component2052037
display id: component2052037
version: 1
top level: BBa_K292001
access: public
definition: https://synbiohub.org/public/igem/BBa_B0011/1

BBa_B0030
type: Component
persistent identity: component2052041
display id: component2052041
version: 1
top level: BBa_K292001
access: public
definition: BBa_B0030

BBa_B0012
type: Component
persistent identity: component2052033
display id: component2052033
version: 1
top level: BBa_K292001
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation2052041
display id: annotation2052041
version: 1
top level: BBa_K292001
location: https://synbiohub.org/public/igem/BBa_K292001/annotation2052041/range2052041/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_K292001/annotation2052041/range2052041/1
type: Range
persistent identity: range2052041
display id: range2052041
version: 1
direction: direction/forward
top level: BBa_K292001
start: 147
end: 161
orientation: inline

BBa_B0011
type: SequenceAnnotation
persistent identity: annotation2052037
display id: annotation2052037
version: 1
top level: BBa_K292001
location: https://synbiohub.org/public/igem/BBa_K292001/annotation2052037/range2052037/1
component: BBa_B0011

https://synbiohub.org/public/igem/BBa_K292001/annotation2052037/range2052037/1
type: Range
persistent identity: range2052037
display id: range2052037
version: 1
direction: direction/forward
top level: BBa_K292001
start: 50
end: 95
orientation: inline

BBa_J23100
type: SequenceAnnotation
persistent identity: annotation2052039
display id: annotation2052039
version: 1
top level: BBa_K292001
location: https://synbiohub.org/public/igem/BBa_K292001/annotation2052039/range2052039/1
component: BBa_J23100

https://synbiohub.org/public/igem/BBa_K292001/annotation2052039/range2052039/1
type: Range
persistent identity: range2052039
display id: range2052039
version: 1
direction: direction/reverse
top level: BBa_K292001
start: 104
end: 138
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2052033
display id: annotation2052033
version: 1
top level: BBa_K292001
location: https://synbiohub.org/public/igem/BBa_K292001/annotation2052033/range2052033/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K292001/annotation2052033/range2052033/1
type: Range
persistent identity: range2052033
display id: range2052033
version: 1
direction: direction/forward
top level: BBa_K292001
start: 1
end: 41
orientation: inline

BBa_J56016
type: ComponentDefinition
persistent identity: BBa J56016
display id: BBa_J56016
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Rbs-orig - ribosome binding site
creator: Matt Eames
created: 2006-10-31T12:00:00Z
modified: 2015-08-31T03:22:59Z
discontinued: false
dominant: false
experience: experience/None
group u list: _79_98_
m user id: 0
owner id: 1132
owning group id: 98
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Ribosome binding site for transcription initiation
mutable notes: None
mutable provenance: Synthesized by DNA 2.0
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J56016_sequence/1

BBa_J61120
type: ComponentDefinition
persistent identity: BBa J61120
display id: BBa_J61120
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ribosome Binding Site Family Member
creator: John Anderson
created: 2007-04-24T11:00:00Z
modified: 2015-08-31T01:59:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable description: {{JCA_Arkin_RBSFamily}}
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J61120_sequence/1

BBa_J61104
type: ComponentDefinition
persistent identity: BBa J61104
display id: BBa_J61104
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ribosome Binding Site Family Member
creator: John Anderson
created: 2007-04-23T11:00:00Z
modified: 2015-08-31T02:03:00Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable description: {{JCA_Arkin_RBSFamily}}
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J61104_sequence/1

BBa_I766557
type: ComponentDefinition
persistent identity: BBa I766557
display id: BBa_I766557
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pSte5 (Weak) Promoter
creator: Nili Sommovilla
created: 2007-08-15T11:00:00Z
modified: 2015-08-31T04:08:10Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _155_
m user id: 0
owner id: 1931
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: low expression, constitutive promoter in yeast
mutable notes: none
mutable provenance: endogenous promoter for Sterile 5
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I766557_sequence/1

BBa_J61147
type: ComponentDefinition
persistent identity: BBa J61147
display id: BBa_J61147
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: {rbs1} Library Member in BBb
creator: John Anderson
created: 2007-09-03T11:00:00Z
modified: 2015-08-31T01:56:25Z
discontinued: false
dominant: false
experience: experience/None
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: {{BBbRBS1Family}}
mutable notes: {{BerkiGEM2007-Biobrick2.0Intro}}
mutable provenance: Synthetic
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J61147_sequence/1

BBa_J23109
type: ComponentDefinition
persistent identity: BBa J23109
display id: BBa_J23109
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: constitutive promoter family member
creator: John Anderson
created: 2006-08-16T11:00:00Z
modified: 2015-08-31T04:08:40Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: true
mutable description: Later
mutable notes: N/A
mutable provenance: Later
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J23109_sequence/1

BBa_K103015
type: ComponentDefinition
persistent identity: BBa K103015
display id: BBa_K103015
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: consensus RBS
creator: Michael Lower
created: 2008-10-08T11:00:00Z
modified: 2015-05-08T01:08:47Z
discontinued: false
dominant: false
experience: experience/None
group u list: _180_
m user id: 0
owner id: 2582
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: strong RBS
sequence: https://synbiohub.org/public/igem/BBa_K103015_sequence/1

strong RBS
type: SequenceAnnotation
persistent identity: annotation1979577
display id: annotation1979577
version: 1
top level: BBa_K103015
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K103015/annotation1979577/range1979577/1

https://synbiohub.org/public/igem/BBa_K103015/annotation1979577/range1979577/1
type: Range
persistent identity: range1979577
display id: range1979577
version: 1
direction: direction/forward
top level: BBa_K103015
start: 1
end: 6
orientation: inline

const.
type: ComponentDefinition
persistent identity: BBa R1075
display id: BBa_R1075
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Constitutive Promoter II
creator: Chris Walsh (Fighting Darwins)
created: 2004-01-27T12:00:00Z
modified: 2015-05-08T01:14:15Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: constitutive promoter which outputs .4 TIPs point mutants of wild type rrnB promoter P1
mutable provenance: Gaal, Gourse, et al.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -10
sequence annotation: -35
sequence: https://synbiohub.org/public/igem/BBa_R1075_sequence/1

-10
type: SequenceAnnotation
persistent identity: annotation309020
display id: annotation309020
version: 1
top level: const.
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R1075/annotation309020/range309020/1

https://synbiohub.org/public/igem/BBa_R1075/annotation309020/range309020/1
type: Range
persistent identity: range309020
display id: range309020
version: 1
direction: direction/forward
top level: const.
start: 35
end: 40
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation309024
display id: annotation309024
version: 1
top level: const.
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R1075/annotation309024/range309024/1

https://synbiohub.org/public/igem/BBa_R1075/annotation309024/range309024/1
type: Range
persistent identity: range309024
display id: range309024
version: 1
direction: direction/forward
top level: const.
start: 15
end: 20
orientation: inline

BBa_K1467101
type: ComponentDefinition
persistent identity: BBa K1467101
display id: BBa_K1467101
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: GoldenGate compatible 35s Promoter
creator: Cara Deal
created: 2014-09-23T11:00:00Z
modified: 2015-05-29T01:36:07Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1846_
m user id: 4206
owner id: 21550
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: 35S is a plant specific promoter obtained from Cauliflower Mosaic Virus. The part is intended for use as a constitutive promoter for gene expression experiments. The part is compatible with GoldenGate MoClo Assembly Standard (free from BsaI and BpiI recognition sequences).
mutable notes: Domestication for GoldenGate (Removal of internal BsaI and BpiI recognition sequences).
mutable provenance: Cauliflower Mosaic Virus (CaMV)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K1467101_sequence/1

BBa_J61117
type: ComponentDefinition
persistent identity: BBa J61117
display id: BBa_J61117
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ribosome Binding Site Family Member
creator: John Anderson
created: 2007-04-23T11:00:00Z
modified: 2015-08-31T01:59:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable description: {{JCA_Arkin_RBSFamily}}
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J61117_sequence/1

BBa_J61122
type: ComponentDefinition
persistent identity: BBa J61122
display id: BBa_J61122
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ribosome Binding Site Family Member
creator: John Anderson
created: 2007-04-24T11:00:00Z
modified: 2015-08-31T01:59:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: {{JCA_Arkin_RBSFamily}}
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J61122_sequence/1

BBa_M13104
type: ComponentDefinition
persistent identity: BBa M13104
display id: BBa_M13104
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: M13K07 gene IV promoter
creator: Natalie Kuldell
created: 2006-12-15T12:00:00Z
modified: 2015-05-08T01:13:55Z
discontinued: false
dominant: false
group u list: _45_
m user id: 0
owner id: 314
owning group id: 1
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This part is from the bacteriophage M13 genome, bp 4055-4103 in M13K07. It directs transcription of M13 gene IV (BBa_M13504, BBa_M13004). Its identity as a promoter was described in Gene (1980) 11:129-148 based on -10,-35 homology and fragment of genome able to bind RNAP.
mutable notes: none
mutable provenance: New England Biolabs M13K07. Sequenced in 2002 by L. Panganaban.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_M13104_sequence/1

pT7
type: ComponentDefinition
persistent identity: BBa I719005
display id: BBa_I719005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: T7 Promoter
creator: Imperial 2007
created: 2007-10-23T11:00:00Z
modified: 2015-08-31T04:07:53Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _128_
m user id: 0
owner id: 2097
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: Just a T7 Promoter
mutable notes: None
mutable provenance: ---
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I719005_sequence/1

BBa_J23102
type: ComponentDefinition
persistent identity: BBa J23102
display id: BBa_J23102
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: constitutive promoter family member
creator: John Anderson
created: 2006-08-03T11:00:00Z
modified: 2015-08-31T04:08:40Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: replace later
mutable notes: N/A
mutable provenance: isolated from library of promoters
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J23102_sequence/1

BBa_I723019
type: ComponentDefinition
persistent identity: BBa I723019
display id: BBa_I723019
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS for XylR
creator: Scott Ramsay
created: 2007-10-24T11:00:00Z
modified: 2015-08-31T04:07:54Z
discontinued: false
dominant: false
experience: experience/None
group u list: _126_
m user id: 0
owner id: 2140
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is the naturally-occurring RBS found in the regulatory region of the DntR gene. gataaaaacaagaggaaaacaa
mutable notes: [Chistine/Maia]
mutable provenance: [Christine/Maia]
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_I723019_sequence/1

RBS
type: SequenceAnnotation
persistent identity: annotation1955263
display id: annotation1955263
version: 1
top level: BBa_I723019
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_I723019/annotation1955263/range1955263/1

https://synbiohub.org/public/igem/BBa_I723019/annotation1955263/range1955263/1
type: Range
persistent identity: range1955263
display id: range1955263
version: 1
direction: direction/forward
top level: BBa_I723019
start: 1
end: 20
orientation: inline

BBa_J61144
type: ComponentDefinition
persistent identity: BBa J61144
display id: BBa_J61144
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: {rbs1} Library Member in BBb
creator: John Anderson
created: 2007-09-03T11:00:00Z
modified: 2015-08-31T01:56:25Z
discontinued: false
dominant: false
experience: experience/None
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: {{BBbRBS1Family}}
mutable notes: {{BerkiGEM2007-Biobrick2.0Intro}}
mutable provenance: Synthetic
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J61144_sequence/1

pTEF
type: ComponentDefinition
persistent identity: BBa K124001
display id: BBa_K124001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Yeast TEF2 Constitutive Promoter
creator: John Szymanski
created: 2008-06-30T11:00:00Z
modified: 2015-05-08T01:09:44Z
discontinued: true
dominant: false
experience: experience/None
group u list: _267_
m user id: 0
owner id: 2512
owning group id: 58
part status: Released HQ 2013
sample status: Discontinued
status: status/Deleted
bookmark: false
mutable description: This is a mid-high level constitutive promoter from the yeast gene TEF2.
mutable notes: none
mutable provenance: Mumberg, Muller, and Funk. p413 pTEF http://dx.doi.org/10.1016/0378-1119(95)00037-7
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K124001_sequence/1

BBa_K1585119
type: ComponentDefinition
persistent identity: BBa K1585119
display id: BBa_K1585119
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Anderson Promoter with lacI binding site
creator: Tobias Schwanemann
created: 2015-08-31T11:00:00Z
modified: 2015-09-01T02:33:36Z
discontinued: false
dominant: false
experience: experience/Fails
group u list: _2002_
m user id: 24474
owner id: 24474
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Promoters of the Anderson Promoter Collection with lacI binding site derived from [http://parts.igem.org/Part:BBa_R0010 R_0010] to make them [http://openwetware.org/wiki/IPTG IPTG] iducible.
mutable notes: We choosed lacI-binding-site of part BBa_R0010 because we assumed older parts would be more reliable. The part is shipped in backbone [http://parts.igem.org/Part:BBa_J61002 J61002] because the functionality is directly visible by adding IPTG. RFP ([http://parts.igem.org/Part:BBa_E1010 E1010]) should get expressed then.
mutable provenance: Assambly of [http://parts.igem.org/Part:BBa_J23119 J23119] and lacI-binding-site of [http://parts.igem.org/Part:BBa_R0010 R_0010] The "lacI binding site" of Part [http://parts.igem.org/Part:BBa_R0010 R_0010] differs from lacO from Part [http://parts.igem.org/Part:BBa_J33207 BBa_J33207].
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
component: BBa_K1585999
component: BBa_J23119
sequence annotation: BBa_J23119
sequence annotation: BBa_K1585999
sequence: https://synbiohub.org/public/igem/BBa_K1585119_sequence/1

BBa_K1585999
type: Component
persistent identity: component2441721
display id: component2441721
version: 1
top level: BBa_K1585119
access: public
definition: https://synbiohub.org/public/igem/BBa_K1585999/1

BBa_J23119
type: Component
persistent identity: component2441719
display id: component2441719
version: 1
top level: BBa_K1585119
access: public
definition: BBa_J23119

BBa_J23119
type: SequenceAnnotation
persistent identity: annotation2441719
display id: annotation2441719
version: 1
top level: BBa_K1585119
location: https://synbiohub.org/public/igem/BBa_K1585119/annotation2441719/range2441719/1
component: BBa_J23119

https://synbiohub.org/public/igem/BBa_K1585119/annotation2441719/range2441719/1
type: Range
persistent identity: range2441719
display id: range2441719
version: 1
direction: direction/reverse
top level: BBa_K1585119
start: 1
end: 35
orientation: inline

BBa_K1585999
type: SequenceAnnotation
persistent identity: annotation2441721
display id: annotation2441721
version: 1
top level: BBa_K1585119
location: https://synbiohub.org/public/igem/BBa_K1585119/annotation2441721/range2441721/1
component: BBa_K1585999

https://synbiohub.org/public/igem/BBa_K1585119/annotation2441721/range2441721/1
type: Range
persistent identity: range2441721
display id: range2441721
version: 1
direction: direction/reverse
top level: BBa_K1585119
start: 44
end: 78
orientation: inline

BBa_K121016
type: ComponentDefinition
persistent identity: BBa K121016
display id: BBa_K121016
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Tokyo Standard prefix suffix RBS
creator: Shotaro Ayukawa
created: 2008-10-25T11:00:00Z
modified: 2015-05-08T01:09:41Z
discontinued: false
dominant: false
experience: experience/None
group u list: _220_
m user id: 0
owner id: 765
owning group id: 76
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Restriction sites of Sal I, Bam HI, Bgl II, and Mlu I in front of strong RBS
mutable notes: We designed new prefix(Sal I and Bam HI) and suffix(Bgl II and Mul I) for insertions of promoters and regulator binding sites in front of RBS.
mutable provenance: RBS is from BBa_0030
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: B0030
sequence annotation: Bgl II
sequence annotation: Sal I
sequence annotation: Mlu I
sequence annotation: Bam HI
sequence: https://synbiohub.org/public/igem/BBa_K121016_sequence/1

B0030
type: SequenceAnnotation
persistent identity: annotation1988216
display id: annotation1988216
version: 1
top level: BBa_K121016
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K121016/annotation1988216/range1988216/1

https://synbiohub.org/public/igem/BBa_K121016/annotation1988216/range1988216/1
type: Range
persistent identity: range1988216
display id: range1988216
version: 1
direction: direction/forward
top level: BBa_K121016
start: 50
end: 64
orientation: inline

Bgl II
type: SequenceAnnotation
persistent identity: annotation1988248
display id: annotation1988248
version: 1
top level: BBa_K121016
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K121016/annotation1988248/range1988248/1

https://synbiohub.org/public/igem/BBa_K121016/annotation1988248/range1988248/1
type: Range
persistent identity: range1988248
display id: range1988248
version: 1
direction: direction/forward
top level: BBa_K121016
start: 26
end: 31
orientation: inline

Sal I
type: SequenceAnnotation
persistent identity: annotation1988246
display id: annotation1988246
version: 1
top level: BBa_K121016
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K121016/annotation1988246/range1988246/1

https://synbiohub.org/public/igem/BBa_K121016/annotation1988246/range1988246/1
type: Range
persistent identity: range1988246
display id: range1988246
version: 1
direction: direction/forward
top level: BBa_K121016
start: 1
end: 6
orientation: inline

Mlu I
type: SequenceAnnotation
persistent identity: annotation1988250
display id: annotation1988250
version: 1
top level: BBa_K121016
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K121016/annotation1988250/range1988250/1

https://synbiohub.org/public/igem/BBa_K121016/annotation1988250/range1988250/1
type: Range
persistent identity: range1988250
display id: range1988250
version: 1
direction: direction/forward
top level: BBa_K121016
start: 41
end: 46
orientation: inline

Bam HI
type: SequenceAnnotation
persistent identity: annotation1988247
display id: annotation1988247
version: 1
top level: BBa_K121016
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K121016/annotation1988247/range1988247/1

https://synbiohub.org/public/igem/BBa_K121016/annotation1988247/range1988247/1
type: Range
persistent identity: range1988247
display id: range1988247
version: 1
direction: direction/forward
top level: BBa_K121016
start: 15
end: 20
orientation: inline

BBa_B0075
type: ComponentDefinition
persistent identity: BBa B0075
display id: BBa_B0075
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Orthogonal RBS
creator: Felix Moser
created: 2008-01-17T12:00:00Z
modified: 2015-08-31T04:07:21Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_
m user id: 0
owner id: 2398
owning group id: 11
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Predicted by Lon Chubiz to be RBS sequence with strongest affinity for T7 ribosomes.
mutable notes: initial AA may be unnecessary
mutable provenance: Lon Chubiz
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: rbs
sequence: https://synbiohub.org/public/igem/BBa_B0075_sequence/1

rbs
type: SequenceAnnotation
persistent identity: annotation1959963
display id: annotation1959963
version: 1
top level: BBa_B0075
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_B0075/annotation1959963/range1959963/1

https://synbiohub.org/public/igem/BBa_B0075/annotation1959963/range1959963/1
type: Range
persistent identity: range1959963
display id: range1959963
version: 1
direction: direction/forward
top level: BBa_B0075
start: 1
end: 8
orientation: inline

revPlam
type: ComponentDefinition
persistent identity: BBa I742126
display id: BBa_I742126
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Reverse lambda cI-regulated promoter
creator: Caroline Dahl
created: 2007-10-21T11:00:00Z
modified: 2015-08-31T04:08:02Z
discontinued: false
dominant: false
experience: experience/None
group u list: _123_
m user id: 0
owner id: 1968
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is an exact 'upside down' version of BBa_R0051. The promoter has two DNA binding sites for lambda cI repressor BBa_C0051. By adding this part, we wish to emphasise the possibility of engineering both DNA strands, and the additional regulatory potential that it gives.
mutable notes: Any 'upside down' (reverse complementary) sequences made by PCR amplification need be made with suffix and prefix is opposite order with respect to the original default sequence.
mutable provenance: See BBa_R0051.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I742126_sequence/1

BBa_J23101
type: ComponentDefinition
persistent identity: BBa J23101
display id: BBa_J23101
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: constitutive promoter family member
creator: John Anderson
created: 2006-08-03T11:00:00Z
modified: 2015-08-31T04:08:40Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: later
mutable notes: N/A
mutable provenance: later
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J23101_sequence/1

BBa_K256020
type: ComponentDefinition
persistent identity: BBa K256020
display id: BBa_K256020
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: J23119:HO1
creator: iGEM09_NTU-Singapore
created: 2009-10-18T11:00:00Z
modified: 2015-05-08T01:11:41Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _359_
m user id: 0
owner id: 4864
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: Generator of Heme Oxygenase (HO1)
mutable notes: --
mutable provenance: --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0034
component: BBa_I15008
component: BBa_J23119
component: BBa_B0015
sequence annotation: BBa_B0034
sequence annotation: BBa_I15008
sequence annotation: BBa_J23119
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_K256020_sequence/1

BBa_B0034
type: Component
persistent identity: component2256390
display id: component2256390
version: 1
top level: BBa_K256020
access: public
definition: BBa_B0034

BBa_I15008
type: Component
persistent identity: component2256392
display id: component2256392
version: 1
top level: BBa_K256020
access: public
definition: https://synbiohub.org/public/igem/BBa_I15008/1

BBa_J23119
type: Component
persistent identity: component2256388
display id: component2256388
version: 1
top level: BBa_K256020
access: public
definition: BBa_J23119

BBa_B0015
type: Component
persistent identity: component2256399
display id: component2256399
version: 1
top level: BBa_K256020
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2256390
display id: annotation2256390
version: 1
top level: BBa_K256020
location: https://synbiohub.org/public/igem/BBa_K256020/annotation2256390/range2256390/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K256020/annotation2256390/range2256390/1
type: Range
persistent identity: range2256390
display id: range2256390
version: 1
direction: direction/reverse
top level: BBa_K256020
start: 44
end: 55
orientation: inline

BBa_I15008
type: SequenceAnnotation
persistent identity: annotation2256392
display id: annotation2256392
version: 1
top level: BBa_K256020
location: https://synbiohub.org/public/igem/BBa_K256020/annotation2256392/range2256392/1
component: BBa_I15008

https://synbiohub.org/public/igem/BBa_K256020/annotation2256392/range2256392/1
type: Range
persistent identity: range2256392
display id: range2256392
version: 1
direction: direction/reverse
top level: BBa_K256020
start: 62
end: 812
orientation: inline

BBa_J23119
type: SequenceAnnotation
persistent identity: annotation2256388
display id: annotation2256388
version: 1
top level: BBa_K256020
location: https://synbiohub.org/public/igem/BBa_K256020/annotation2256388/range2256388/1
component: BBa_J23119

https://synbiohub.org/public/igem/BBa_K256020/annotation2256388/range2256388/1
type: Range
persistent identity: range2256388
display id: range2256388
version: 1
direction: direction/reverse
top level: BBa_K256020
start: 1
end: 35
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2256399
display id: annotation2256399
version: 1
top level: BBa_K256020
location: https://synbiohub.org/public/igem/BBa_K256020/annotation2256399/range2256399/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K256020/annotation2256399/range2256399/1
type: Range
persistent identity: range2256399
display id: range2256399
version: 1
direction: direction/reverse
top level: BBa_K256020
start: 821
end: 949
orientation: inline

BBa_J64997
type: ComponentDefinition
persistent identity: BBa J64997
display id: BBa_J64997
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: T7 consensus -10 and rest
creator: Sai Duriseti
created: 2007-03-25T11:00:00Z
modified: 2015-05-08T01:08:18Z
discontinued: false
dominant: false
experience: experience/None
group u list: _98_
m user id: 0
owner id: 1428
owning group id: 98
sample status: Not in stock
status: status/Unavailable
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: T7-10
sequence: https://synbiohub.org/public/igem/BBa_J64997_sequence/1

T7-10
type: SequenceAnnotation
persistent identity: annotation1921746
display id: annotation1921746
version: 1
top level: BBa_J64997
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J64997/annotation1921746/range1921746/1

https://synbiohub.org/public/igem/BBa_J64997/annotation1921746/range1921746/1
type: Range
persistent identity: range1921746
display id: range1921746
version: 1
direction: direction/forward
top level: BBa_J64997
start: 1
end: 19
orientation: inline

BBa_K292000
type: ComponentDefinition
persistent identity: BBa K292000
display id: BBa_K292000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Double terminator + constitutive promoter
creator: David Charoy
created: 2009-10-17T11:00:00Z
modified: 2015-05-08T01:11:49Z
discontinued: false
dominant: true
group u list: _394_
m user id: 0
owner id: 5212
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part contains a double terminator and a constitutive prokaryotic promoter. It is a simple promoter to begin gene transcription. For Sup???biotech-Paris (2009) team this part is used after to be added to a recombinant lambda phage to begin the transcription of synthetic genes inside the Lambda phage genome.
mutable notes: N/A
mutable provenance: BBa_B0014 : Designed by Reshma Shetty Group: Registry (2003-07-16) BBa_J23100 : Designed by John Anderson Group: iGEM2006_Berkeley (2006-08-04)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_B0012
component: BBa_B0011
component: BBa_J23100
sequence annotation: BBa_B0011
sequence annotation: BBa_B0012
sequence annotation: BBa_J23100
sequence: https://synbiohub.org/public/igem/BBa_K292000_sequence/1

BBa_B0012
type: Component
persistent identity: component2059157
display id: component2059157
version: 1
top level: BBa_K292000
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0011
type: Component
persistent identity: component2059161
display id: component2059161
version: 1
top level: BBa_K292000
access: public
definition: https://synbiohub.org/public/igem/BBa_B0011/1

BBa_J23100
type: Component
persistent identity: component2059163
display id: component2059163
version: 1
top level: BBa_K292000
access: public
definition: BBa_J23100

BBa_B0011
type: SequenceAnnotation
persistent identity: annotation2059161
display id: annotation2059161
version: 1
top level: BBa_K292000
location: https://synbiohub.org/public/igem/BBa_K292000/annotation2059161/range2059161/1
component: BBa_B0011

https://synbiohub.org/public/igem/BBa_K292000/annotation2059161/range2059161/1
type: Range
persistent identity: range2059161
display id: range2059161
version: 1
direction: direction/forward
top level: BBa_K292000
start: 50
end: 95
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2059157
display id: annotation2059157
version: 1
top level: BBa_K292000
location: https://synbiohub.org/public/igem/BBa_K292000/annotation2059157/range2059157/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K292000/annotation2059157/range2059157/1
type: Range
persistent identity: range2059157
display id: range2059157
version: 1
direction: direction/forward
top level: BBa_K292000
start: 1
end: 41
orientation: inline

BBa_J23100
type: SequenceAnnotation
persistent identity: annotation2059163
display id: annotation2059163
version: 1
top level: BBa_K292000
location: https://synbiohub.org/public/igem/BBa_K292000/annotation2059163/range2059163/1
component: BBa_J23100

https://synbiohub.org/public/igem/BBa_K292000/annotation2059163/range2059163/1
type: Range
persistent identity: range2059163
display id: range2059163
version: 1
direction: direction/reverse
top level: BBa_K292000
start: 104
end: 138
orientation: inline

BBa_K1585105
type: ComponentDefinition
persistent identity: BBa K1585105
display id: BBa_K1585105
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Anderson Promoter with lacI binding site
creator: Tobias Schwanemann
created: 2015-08-31T11:00:00Z
modified: 2015-09-01T02:03:04Z
discontinued: false
dominant: false
experience: experience/Fails
group u list: _2002_
m user id: 24474
owner id: 24474
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Promoters of the Anderson Promoter Collection with lacI binding site derived from [http://parts.igem.org/Part:BBa_R0010 R_0010] to make them [http://openwetware.org/wiki/IPTG IPTG] iducible.
mutable notes: We choosed lacI-binding-site of part BBa_R0010 because we assumed older parts would be more reliable. The part is shipped in backbone [http://parts.igem.org/Part:BBa_J61002 J61002] because the functionality is directly visible by adding IPTG. RFP ([http://parts.igem.org/Part:BBa_E1010 E1010]) should get expressed then.
mutable provenance: Assambly of [http://parts.igem.org/Part:BBa_J23103 J23103] and lacI-binding-site of [http://parts.igem.org/Part:BBa_R0010 R_0010] The "lacI binding site" of Part [http://parts.igem.org/Part:BBa_R0010 R_0010] differs from lacO from Part [http://parts.igem.org/Part:BBa_J33207 BBa_J33207].
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
component: BBa_K1585999
component: BBa_J23105
sequence annotation: BBa_K1585999
sequence annotation: BBa_J23105
sequence: https://synbiohub.org/public/igem/BBa_K1585105_sequence/1

BBa_K1585999
type: Component
persistent identity: component2441416
display id: component2441416
version: 1
top level: BBa_K1585105
access: public
definition: https://synbiohub.org/public/igem/BBa_K1585999/1

BBa_J23105
type: Component
persistent identity: component2441414
display id: component2441414
version: 1
top level: BBa_K1585105
access: public
definition: BBa_J23105

BBa_K1585999
type: SequenceAnnotation
persistent identity: annotation2441416
display id: annotation2441416
version: 1
top level: BBa_K1585105
location: https://synbiohub.org/public/igem/BBa_K1585105/annotation2441416/range2441416/1
component: BBa_K1585999

https://synbiohub.org/public/igem/BBa_K1585105/annotation2441416/range2441416/1
type: Range
persistent identity: range2441416
display id: range2441416
version: 1
direction: direction/reverse
top level: BBa_K1585105
start: 44
end: 78
orientation: inline

BBa_J23105
type: SequenceAnnotation
persistent identity: annotation2441414
display id: annotation2441414
version: 1
top level: BBa_K1585105
location: https://synbiohub.org/public/igem/BBa_K1585105/annotation2441414/range2441414/1
component: BBa_J23105

https://synbiohub.org/public/igem/BBa_K1585105/annotation2441414/range2441414/1
type: Range
persistent identity: range2441414
display id: range2441414
version: 1
direction: direction/reverse
top level: BBa_K1585105
start: 1
end: 35
orientation: inline

BBa_K777007
type: ComponentDefinition
persistent identity: BBa K777007
display id: BBa_K777007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Tar receptor under the control of constitutive promoter J23113
creator: Bianca Genenncher
created: 2012-09-20T11:00:00Z
modified: 2015-05-08T01:13:16Z
discontinued: false
dominant: false
group u list: _1029_
m user id: 0
owner id: 11946
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: The transmembrane chemoreceptor Tar of E. coli mediates chemotaxis towards aspartate and exists as a functional homodimer. Here we used a set of 8 constitutive [http://partsregistry.org/Part:BBa_J23113 Anderson promoters] from the 2006 Berkeley group to test how different levels of constitutive ''Tar'' expression affect the chemotaxis of ''E. coli''.
mutable notes: * Genomic sequence was amplified using the following primers. ** Primers PCR: Base pairs in caps represent the respective restriction sites followed by either prefix or suffix. Primers were provided by SIGMA. *** Fwd: Tar for + prefix: 5?? actGAATTCgcggccgctTCTAGAtgattaaccgtatccgcgtagtc 3?? *** Rev: Tar rev + suffix: 5?? tcCTGCAGcggccgctACTAGTcaaaatgtttcccagtttggatc 3?? * One XbaI site was found at position 420. The restriction site was changed from TCTAGt to CCTAGt via QuikChange PCR. ** Primers QuikChange (QC): Primers were provided by SIGMA. *** Fwd: TarQC for: 5?? ACTGATTGATTAcCTAGATTATGGCAATACTGGAG 3?? *** Rev: TarQC rev: 5?? TGCCATAATCTAGgTAATCAATCAGTTCAGTTAAC 3?? (the uncapitalized letter induces the mutation for removal of the XbaI site)
mutable provenance: *The part was amplified from genomic DNA of ''E. coli'' str. K-12 substr. DH10B, complete genome (CP000948.1). *J23113 information was taken from the partsregistry and physical DNA from the 2012 distribution kit.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K777000
component: BBa_J23113
sequence annotation: BBa_J23113
sequence annotation: BBa_K777000
sequence: https://synbiohub.org/public/igem/BBa_K777007_sequence/1

BBa_K777000
type: Component
persistent identity: component2189403
display id: component2189403
version: 1
top level: BBa_K777007
access: public
definition: BBa_K777000

BBa_J23113
type: Component
persistent identity: component2189399
display id: component2189399
version: 1
top level: BBa_K777007
access: public
definition: BBa_J23113

BBa_J23113
type: SequenceAnnotation
persistent identity: annotation2189399
display id: annotation2189399
version: 1
top level: BBa_K777007
location: https://synbiohub.org/public/igem/BBa_K777007/annotation2189399/range2189399/1
component: BBa_J23113

https://synbiohub.org/public/igem/BBa_K777007/annotation2189399/range2189399/1
type: Range
persistent identity: range2189399
display id: range2189399
version: 1
direction: direction/reverse
top level: BBa_K777007
start: 1
end: 35
orientation: inline

BBa_K777000
type: SequenceAnnotation
persistent identity: annotation2189403
display id: annotation2189403
version: 1
top level: BBa_K777007
location: https://synbiohub.org/public/igem/BBa_K777007/annotation2189403/range2189403/1
component: BBa_K777000

https://synbiohub.org/public/igem/BBa_K777007/annotation2189403/range2189403/1
type: Range
persistent identity: range2189403
display id: range2189403
version: 1
direction: direction/reverse
top level: BBa_K777007
start: 42
end: 1703
orientation: inline

BBa_K777104
type: ComponentDefinition
persistent identity: BBa K777104
display id: BBa_K777104
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: flhDC operon under the control of constitutive promoter J23106
creator: Team Goettingen
created: 2012-09-18T11:00:00Z
modified: 2015-05-08T01:13:16Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1029_
m user id: 0
owner id: 12106
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: The ''flhDC'' operon is the master regulator of motility and chemotaxis in ''E. coli''. FlhDC forms heterotetramers and activates class II operons in concert with sigma factor 70. Among the gene products of class II operons are several components of the flagellum and the alternative sigma factor FliA which is essential for the transcription of class III genes. Here we used a set of 8 constitutive Anderson-promoters from the 2006 Berkeley group to test how different levels of constitutive ''flhDC'' expression affect the motility of ''E. coli''.
mutable notes: * The PstI restriction site in the ''flhDC'' sequence was mutated to CTGCGG using the reverse primer * ''flhDC'' was amplified from genomic DNA of ''E. coli'' DH10B via PCR using the following primers: ** Fwd: 5'-actgaattcgcggccgcttctagatgcatacctccgagttgctg-3' ** Rev: 5'-tcctgcagcggccgctactagttactgcccgggatggcggttgacataagcCgcaggcaaagctgccaacag-3' (the capitalized "C" induces the mutation for removal of the PstI site)
mutable provenance: * The part was amplified from genomic DNA of ''E. coli'' str. K-12 substr. DH10B, complete genome (CP000948.1). * J23106 information was taken from the partsregistry and physical DNA from the 2012 distribution kit.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_J23106
component: BBa_K777100
sequence annotation: BBa_K777100
sequence annotation: BBa_J23106
sequence: https://synbiohub.org/public/igem/BBa_K777104_sequence/1

BBa_J23106
type: Component
persistent identity: component2187039
display id: component2187039
version: 1
top level: BBa_K777104
access: public
definition: BBa_J23106

BBa_K777100
type: Component
persistent identity: component2187047
display id: component2187047
version: 1
top level: BBa_K777104
access: public
definition: https://synbiohub.org/public/igem/BBa_K777100/1

BBa_K777100
type: SequenceAnnotation
persistent identity: annotation2187047
display id: annotation2187047
version: 1
top level: BBa_K777104
location: https://synbiohub.org/public/igem/BBa_K777104/annotation2187047/range2187047/1
component: BBa_K777100

https://synbiohub.org/public/igem/BBa_K777104/annotation2187047/range2187047/1
type: Range
persistent identity: range2187047
display id: range2187047
version: 1
direction: direction/reverse
top level: BBa_K777104
start: 42
end: 910
orientation: inline

BBa_J23106
type: SequenceAnnotation
persistent identity: annotation2187039
display id: annotation2187039
version: 1
top level: BBa_K777104
location: https://synbiohub.org/public/igem/BBa_K777104/annotation2187039/range2187039/1
component: BBa_J23106

https://synbiohub.org/public/igem/BBa_K777104/annotation2187039/range2187039/1
type: Range
persistent identity: range2187039
display id: range2187039
version: 1
direction: direction/reverse
top level: BBa_K777104
start: 1
end: 35
orientation: inline

revRBS
type: ComponentDefinition
persistent identity: BBa I742130
display id: BBa_I742130
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Reverse RBS
creator: Caroline Dahl
created: 2007-10-21T11:00:00Z
modified: 2015-08-31T04:08:03Z
discontinued: false
dominant: false
experience: experience/None
group u list: _123_
m user id: 0
owner id: 1968
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: DNA is double-stranded. To translate genes of both strands, you need RBSs in both strands.
mutable notes: Please refer to the original (BB_B0034).
mutable provenance: Please refer to the original (BB_B0034).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_I742130_sequence/1

BBa_M13506
type: ComponentDefinition
persistent identity: BBa M13506
display id: BBa_M13506
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: M13K07 gene VI RBS
creator: Natalie Kuldell
created: 2006-12-14T12:00:00Z
modified: 2015-05-08T01:13:56Z
discontinued: false
dominant: false
experience: experience/None
group u list: _45_
m user id: 0
owner id: 314
owning group id: 1
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This RBS is part of the bacteriophage M13 genome, initiating translation of the gene VI message (BBa_M13006). The part aligns with base pairs 2840-2855 in M13K07 genome. It was identified as the RBS for gene VI message by Wezenbeek et al in Gene (1980) 11: 129-148
mutable notes: none
mutable provenance: New England Biolabs M13K07. Sequenced in 2002 by L. Panganaban
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_M13506_sequence/1

BBa_K1847000
type: ComponentDefinition
persistent identity: BBa K1847000
display id: BBa_K1847000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: promoter-RBS- annexin AV
creator: Anna Fomitcheva, Lisa Baumgartner
created: 2015-08-23T11:00:00Z
modified: 2015-09-17T08:44:38Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2273_
m user id: 26442
owner id: 26442
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Annexin V is member of the annexin family of Ca dependent proteins. Its function is not yet known. It binds to phosphatidylserine in a Ca-dependen fashion.
mutable notes: In combination with RBS B0032 there is no detectable expression of annexin V.
mutable provenance: Synthetic synthesis
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: Annexin V optimized for E. coli
sequence annotation: B0032
sequence annotation: J23114
sequence: https://synbiohub.org/public/igem/BBa_K1847000_sequence/1

Annexin V optimized for E. coli
type: SequenceAnnotation
persistent identity: annotation2437963
display id: annotation2437963
version: 1
top level: BBa_K1847000
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1847000/annotation2437963/range2437963/1

https://synbiohub.org/public/igem/BBa_K1847000/annotation2437963/range2437963/1
type: Range
persistent identity: range2437963
display id: range2437963
version: 1
direction: direction/forward
top level: BBa_K1847000
start: 63
end: 1025
orientation: inline

B0032
type: SequenceAnnotation
persistent identity: annotation2437962
display id: annotation2437962
version: 1
top level: BBa_K1847000
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1847000/annotation2437962/range2437962/1

https://synbiohub.org/public/igem/BBa_K1847000/annotation2437962/range2437962/1
type: Range
persistent identity: range2437962
display id: range2437962
version: 1
direction: direction/forward
top level: BBa_K1847000
start: 44
end: 56
orientation: inline

J23114
type: SequenceAnnotation
persistent identity: annotation2437961
display id: annotation2437961
version: 1
top level: BBa_K1847000
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1847000/annotation2437961/range2437961/1

https://synbiohub.org/public/igem/BBa_K1847000/annotation2437961/range2437961/1
type: Range
persistent identity: range2437961
display id: range2437961
version: 1
direction: direction/forward
top level: BBa_K1847000
start: 1
end: 35
orientation: inline

BBa_J64030
type: ComponentDefinition
persistent identity: BBa J64030
display id: BBa_J64030
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: sopD RBS
creator: Dan Widmaier
created: 2008-01-16T12:00:00Z
modified: 2015-08-31T01:56:26Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _98_
m user id: 0
owner id: 2407
owning group id: 98
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is the natural RBS from the sopD gene in Salmonella Type III Secretion.
mutable notes: This part was designed without consideration for biobrick standard assembly. This RBS has not been explicitly tested for expression rate.
mutable provenance: Salmonella LT2 Genomic DNA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: sopD RBS
sequence: https://synbiohub.org/public/igem/BBa_J64030_sequence/1

sopD RBS
type: SequenceAnnotation
persistent identity: annotation1959634
display id: annotation1959634
version: 1
top level: BBa_J64030
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_J64030/annotation1959634/range1959634/1

https://synbiohub.org/public/igem/BBa_J64030/annotation1959634/range1959634/1
type: Range
persistent identity: range1959634
display id: range1959634
version: 1
direction: direction/forward
top level: BBa_J64030
start: 1
end: 17
orientation: inline

BBa_B0071
type: ComponentDefinition
persistent identity: BBa B0071
display id: BBa_B0071
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Specialized RBS
creator: Barry Canton
created: 2006-03-03T12:00:00Z
modified: 2015-08-31T04:07:21Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_6_
m user id: 0
owner id: 135
owning group id: 6
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This RBS has the modified Shine-Dalgarno sequence, it has a reduced secondary structure relative to using the actual Brink sequence with mixed connective sites flanking it. This RBS is modified from B0071 by reducing the 3' sequence so that the SD to ATG spacing is closer to that which is optimal for E. coli.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_B0071_sequence/1

BBa_K105028
type: ComponentDefinition
persistent identity: BBa K105028
display id: BBa_K105028
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: cyc70 minimal promoter
creator: Michael Wild
created: 2008-10-25T11:00:00Z
modified: 2015-05-08T01:08:52Z
discontinued: false
dominant: false
experience: experience/None
group u list: _253_
m user id: 0
owner id: 3313
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This is a derivate of BBa_K105027. Due to a point mutation in the TATA box region the efficiency of this minimal promoter is only 70 % of the natural yeast CYC1 promoter.
mutable notes: This BioBrick contains the TATA-box with one point mutation and most of the 5'UTR (-139 to -36, relative to the translational start) of the natural CYC1 promoter. A Kozak sequence is not included and has thus to be added! Even if there isn't any need for this BioBrick to be fusible to other protein domains, we stayed with our design.
mutable provenance: Amplification and Mutation from the plasmid pAH3
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: TATA-box
sequence annotation: A->C
sequence: https://synbiohub.org/public/igem/BBa_K105028_sequence/1

TATA-box
type: SequenceAnnotation
persistent identity: annotation1989835
display id: annotation1989835
version: 1
top level: BBa_K105028
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K105028/annotation1989835/range1989835/1

https://synbiohub.org/public/igem/BBa_K105028/annotation1989835/range1989835/1
type: Range
persistent identity: range1989835
display id: range1989835
version: 1
direction: direction/forward
top level: BBa_K105028
start: 17
end: 23
orientation: inline

A->C
type: SequenceAnnotation
persistent identity: annotation1989836
display id: annotation1989836
version: 1
top level: BBa_K105028
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_K105028/annotation1989836/range1989836/1

https://synbiohub.org/public/igem/BBa_K105028/annotation1989836/range1989836/1
type: Range
persistent identity: range1989836
display id: range1989836
version: 1
direction: direction/forward
top level: BBa_K105028
start: 24
end: 24
orientation: inline

BBa_K1614000
type: ComponentDefinition
persistent identity: BBa K1614000
display id: BBa_K1614000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: T7 promoter for expression of functional RNA
creator: Stefan Holderbach
created: 2015-09-13T11:00:00Z
modified: 2015-09-14T12:29:29Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2031_
m user id: 12380
owner id: 16422
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Test
mutable notes: None.
mutable provenance: T7 phage minimal promoter (consensus)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: T7 promoter
sequence: https://synbiohub.org/public/igem/BBa_K1614000_sequence/1

T7 promoter
type: SequenceAnnotation
persistent identity: annotation2454728
display id: annotation2454728
version: 1
top level: BBa_K1614000
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1614000/annotation2454728/range2454728/1

https://synbiohub.org/public/igem/BBa_K1614000/annotation2454728/range2454728/1
type: Range
persistent identity: range2454728
display id: range2454728
version: 1
direction: direction/forward
top level: BBa_K1614000
start: 1
end: 17
orientation: inline

BBa_K1585113
type: ComponentDefinition
persistent identity: BBa K1585113
display id: BBa_K1585113
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Anderson Promoter with lacI binding site
creator: Tobias Schwanemann
created: 2015-08-31T11:00:00Z
modified: 2015-09-01T02:09:36Z
discontinued: false
dominant: false
experience: experience/Fails
group u list: _2002_
m user id: 24474
owner id: 24474
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Promoters of the Anderson Promoter Collection with lacI binding site derived from [http://parts.igem.org/Part:BBa_R0010 R_0010] to make them [http://openwetware.org/wiki/IPTG IPTG] iducible.
mutable notes: We choosed lacI-binding-site of part BBa_R0010 because we assumed older parts would be more reliable. The part is shipped in backbone [http://parts.igem.org/Part:BBa_J61002 J61002] because the functionality is directly visible by adding IPTG. RFP ([http://parts.igem.org/Part:BBa_E1010 E1010]) should get expressed then.
mutable provenance: Assambly of [http://parts.igem.org/Part:BBa_J23110 J23110] and lacI-binding-site of [http://parts.igem.org/Part:BBa_R0010 R_0010] The "lacI binding site" of Part [http://parts.igem.org/Part:BBa_R0010 R_0010] differs from lacO from Part [http://parts.igem.org/Part:BBa_J33207 BBa_J33207].
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
component: BBa_K1585999
component: BBa_J23113
sequence annotation: BBa_J23113
sequence annotation: BBa_K1585999
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