//rbs/prokaryote/constitutive/constitutive
type: Collection
persistent identity: rbs prokaryote constitutive constitutive
display id: rbs_prokaryote_constitutive_constitutive
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
owned by: james
owned by: myers
member: BBa_K1031302
member: 77RBS
member: BBa_K356103
member: BBa_K1031301
member: BBa_K1031221
member: BBa_K121016
member: BBa_K356105
member: BBa_K1732000
member: BBa_K1031114

BBa_K356105
type: ComponentDefinition
persistent identity: BBa K356105
display id: BBa_K356105
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Desinged RBS
creator: Fatemeh Salimi
created: 2010-10-25T11:00:00Z
modified: 2015-05-08T01:12:11Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _467_
m user id: 0
owner id: 6685
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: Designed RBS
mutable notes: primers
mutable provenance: RBS calculator
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_K356105_sequence/1

BBa_K1031221
type: ComponentDefinition
persistent identity: BBa K1031221
display id: BBa_K1031221
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Po-B0031-sfGFP-Terminator (DmpR)
creator: He Shuaixin
created: 2013-09-08T11:00:00Z
modified: 2015-05-08T01:08:47Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1338_
m user id: 0
owner id: 15843
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: Po-31-sfGFP-TT (DmpR)
mutable notes: molecular cloning
mutable provenance: synthesis, lab made
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: sfGFP
sequence annotation: B0015
sequence annotation: Po promoter
sequence annotation: B0031
sequence: https://synbiohub.org/public/igem/BBa_K1031221_sequence/1

sfGFP
type: SequenceAnnotation
persistent identity: annotation2354352
display id: annotation2354352
version: 1
top level: BBa_K1031221
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1031221/annotation2354352/range2354352/1

https://synbiohub.org/public/igem/BBa_K1031221/annotation2354352/range2354352/1
type: Range
persistent identity: range2354352
display id: range2354352
version: 1
direction: direction/forward
top level: BBa_K1031221
start: 200
end: 922
orientation: inline

B0015
type: SequenceAnnotation
persistent identity: annotation2354353
display id: annotation2354353
version: 1
top level: BBa_K1031221
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1031221/annotation2354353/range2354353/1

https://synbiohub.org/public/igem/BBa_K1031221/annotation2354353/range2354353/1
type: Range
persistent identity: range2354353
display id: range2354353
version: 1
direction: direction/forward
top level: BBa_K1031221
start: 931
end: 1059
orientation: inline

Po promoter
type: SequenceAnnotation
persistent identity: annotation2354350
display id: annotation2354350
version: 1
top level: BBa_K1031221
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1031221/annotation2354350/range2354350/1

https://synbiohub.org/public/igem/BBa_K1031221/annotation2354350/range2354350/1
type: Range
persistent identity: range2354350
display id: range2354350
version: 1
direction: direction/forward
top level: BBa_K1031221
start: 1
end: 171
orientation: inline

B0031
type: SequenceAnnotation
persistent identity: annotation2354351
display id: annotation2354351
version: 1
top level: BBa_K1031221
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1031221/annotation2354351/range2354351/1

https://synbiohub.org/public/igem/BBa_K1031221/annotation2354351/range2354351/1
type: Range
persistent identity: range2354351
display id: range2354351
version: 1
direction: direction/forward
top level: BBa_K1031221
start: 180
end: 193
orientation: inline

BBa_K356103
type: ComponentDefinition
persistent identity: BBa K356103
display id: BBa_K356103
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PFS78RBSGFP
creator: Rachael Blackburn
created: 2010-10-23T11:00:00Z
modified: 2015-05-08T01:12:11Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _467_
m user id: 0
owner id: 6685
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: Promoter RBS GFP double terminator
mutable notes: Primer design
mutable provenance: RBS from RBS calculator
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_B0010
component: BBa_K356105
component: BBa_B0012
component: BBa_J23100
component: BBa_E0040
sequence annotation: BBa_K356105
sequence annotation: BBa_B0012
sequence annotation: BBa_B0010
sequence annotation: BBa_E0040
sequence annotation: BBa_J23100
sequence: https://synbiohub.org/public/igem/BBa_K356103_sequence/1

BBa_B0010
type: Component
persistent identity: component2109047
display id: component2109047
version: 1
top level: BBa_K356103
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_K356105
type: Component
persistent identity: component2109044
display id: component2109044
version: 1
top level: BBa_K356103
access: public
definition: BBa_K356105

BBa_B0012
type: Component
persistent identity: component2109049
display id: component2109049
version: 1
top level: BBa_K356103
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_J23100
type: Component
persistent identity: component2109043
display id: component2109043
version: 1
top level: BBa_K356103
access: public
definition: https://synbiohub.org/public/igem/BBa_J23100/1

BBa_E0040
type: Component
persistent identity: component2109046
display id: component2109046
version: 1
top level: BBa_K356103
access: public
definition: https://synbiohub.org/public/igem/BBa_E0040/1

BBa_K356105
type: SequenceAnnotation
persistent identity: annotation2109044
display id: annotation2109044
version: 1
top level: BBa_K356103
location: https://synbiohub.org/public/igem/BBa_K356103/annotation2109044/range2109044/1
component: BBa_K356105

https://synbiohub.org/public/igem/BBa_K356103/annotation2109044/range2109044/1
type: Range
persistent identity: range2109044
display id: range2109044
version: 1
direction: direction/reverse
top level: BBa_K356103
start: 44
end: 57
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2109049
display id: annotation2109049
version: 1
top level: BBa_K356103
location: https://synbiohub.org/public/igem/BBa_K356103/annotation2109049/range2109049/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K356103/annotation2109049/range2109049/1
type: Range
persistent identity: range2109049
display id: range2109049
version: 1
direction: direction/forward
top level: BBa_K356103
start: 880
end: 920
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2109047
display id: annotation2109047
version: 1
top level: BBa_K356103
location: https://synbiohub.org/public/igem/BBa_K356103/annotation2109047/range2109047/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K356103/annotation2109047/range2109047/1
type: Range
persistent identity: range2109047
display id: range2109047
version: 1
direction: direction/forward
top level: BBa_K356103
start: 792
end: 871
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation2109046
display id: annotation2109046
version: 1
top level: BBa_K356103
location: https://synbiohub.org/public/igem/BBa_K356103/annotation2109046/range2109046/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_K356103/annotation2109046/range2109046/1
type: Range
persistent identity: range2109046
display id: range2109046
version: 1
direction: direction/reverse
top level: BBa_K356103
start: 64
end: 783
orientation: inline

BBa_J23100
type: SequenceAnnotation
persistent identity: annotation2109043
display id: annotation2109043
version: 1
top level: BBa_K356103
location: https://synbiohub.org/public/igem/BBa_K356103/annotation2109043/range2109043/1
component: BBa_J23100

https://synbiohub.org/public/igem/BBa_K356103/annotation2109043/range2109043/1
type: Range
persistent identity: range2109043
display id: range2109043
version: 1
direction: direction/reverse
top level: BBa_K356103
start: 1
end: 35
orientation: inline

BBa_K121016
type: ComponentDefinition
persistent identity: BBa K121016
display id: BBa_K121016
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Tokyo Standard prefix suffix RBS
creator: Shotaro Ayukawa
created: 2008-10-25T11:00:00Z
modified: 2015-05-08T01:09:41Z
discontinued: false
dominant: false
experience: experience/None
group u list: _220_
m user id: 0
owner id: 765
owning group id: 76
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Restriction sites of Sal I, Bam HI, Bgl II, and Mlu I in front of strong RBS
mutable notes: We designed new prefix(Sal I and Bam HI) and suffix(Bgl II and Mul I) for insertions of promoters and regulator binding sites in front of RBS.
mutable provenance: RBS is from BBa_0030
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: Bgl II
sequence annotation: Mlu I
sequence annotation: Bam HI
sequence annotation: B0030
sequence annotation: Sal I
sequence: https://synbiohub.org/public/igem/BBa_K121016_sequence/1

Bgl II
type: SequenceAnnotation
persistent identity: annotation1988248
display id: annotation1988248
version: 1
top level: BBa_K121016
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K121016/annotation1988248/range1988248/1

https://synbiohub.org/public/igem/BBa_K121016/annotation1988248/range1988248/1
type: Range
persistent identity: range1988248
display id: range1988248
version: 1
direction: direction/forward
top level: BBa_K121016
start: 26
end: 31
orientation: inline

Mlu I
type: SequenceAnnotation
persistent identity: annotation1988250
display id: annotation1988250
version: 1
top level: BBa_K121016
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K121016/annotation1988250/range1988250/1

https://synbiohub.org/public/igem/BBa_K121016/annotation1988250/range1988250/1
type: Range
persistent identity: range1988250
display id: range1988250
version: 1
direction: direction/forward
top level: BBa_K121016
start: 41
end: 46
orientation: inline

Bam HI
type: SequenceAnnotation
persistent identity: annotation1988247
display id: annotation1988247
version: 1
top level: BBa_K121016
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K121016/annotation1988247/range1988247/1

https://synbiohub.org/public/igem/BBa_K121016/annotation1988247/range1988247/1
type: Range
persistent identity: range1988247
display id: range1988247
version: 1
direction: direction/forward
top level: BBa_K121016
start: 15
end: 20
orientation: inline

B0030
type: SequenceAnnotation
persistent identity: annotation1988216
display id: annotation1988216
version: 1
top level: BBa_K121016
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K121016/annotation1988216/range1988216/1

https://synbiohub.org/public/igem/BBa_K121016/annotation1988216/range1988216/1
type: Range
persistent identity: range1988216
display id: range1988216
version: 1
direction: direction/forward
top level: BBa_K121016
start: 50
end: 64
orientation: inline

Sal I
type: SequenceAnnotation
persistent identity: annotation1988246
display id: annotation1988246
version: 1
top level: BBa_K121016
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K121016/annotation1988246/range1988246/1

https://synbiohub.org/public/igem/BBa_K121016/annotation1988246/range1988246/1
type: Range
persistent identity: range1988246
display id: range1988246
version: 1
direction: direction/forward
top level: BBa_K121016
start: 1
end: 6
orientation: inline

77RBS
type: ComponentDefinition
persistent identity: BBa K356102
display id: BBa_K356102
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PFS77RBSGFP
creator: Fatemeh Salimi
created: 2010-10-23T11:00:00Z
modified: 2015-05-08T01:12:11Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _467_
m user id: 0
owner id: 6685
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: Promoter RBS GFP double terminator
mutable notes: Primer design
mutable provenance: Designed RBS from RBS calculator
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_B0012
component: BBa_J23100
component: BBa_B0010
component: BBa_J61135
component: BBa_E0040
sequence annotation: BBa_B0010
sequence annotation: BBa_J61135
sequence annotation: BBa_B0012
sequence annotation: BBa_J23100
sequence annotation: BBa_E0040
sequence: https://synbiohub.org/public/igem/BBa_K356102_sequence/1

BBa_B0012
type: Component
persistent identity: component2108911
display id: component2108911
version: 1
top level: 77RBS
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_J23100
type: Component
persistent identity: component2108905
display id: component2108905
version: 1
top level: 77RBS
access: public
definition: https://synbiohub.org/public/igem/BBa_J23100/1

BBa_B0010
type: Component
persistent identity: component2108909
display id: component2108909
version: 1
top level: 77RBS
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_J61135
type: Component
persistent identity: component2108906
display id: component2108906
version: 1
top level: 77RBS
access: public
definition: https://synbiohub.org/public/igem/BBa_J61135/1

BBa_E0040
type: Component
persistent identity: component2108908
display id: component2108908
version: 1
top level: 77RBS
access: public
definition: https://synbiohub.org/public/igem/BBa_E0040/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2108909
display id: annotation2108909
version: 1
top level: 77RBS
location: https://synbiohub.org/public/igem/BBa_K356102/annotation2108909/range2108909/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K356102/annotation2108909/range2108909/1
type: Range
persistent identity: range2108909
display id: range2108909
version: 1
direction: direction/forward
top level: 77RBS
start: 790
end: 869
orientation: inline

BBa_J61135
type: SequenceAnnotation
persistent identity: annotation2108906
display id: annotation2108906
version: 1
top level: 77RBS
location: https://synbiohub.org/public/igem/BBa_K356102/annotation2108906/range2108906/1
component: BBa_J61135

https://synbiohub.org/public/igem/BBa_K356102/annotation2108906/range2108906/1
type: Range
persistent identity: range2108906
display id: range2108906
version: 1
direction: direction/reverse
top level: 77RBS
start: 44
end: 55
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2108911
display id: annotation2108911
version: 1
top level: 77RBS
location: https://synbiohub.org/public/igem/BBa_K356102/annotation2108911/range2108911/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K356102/annotation2108911/range2108911/1
type: Range
persistent identity: range2108911
display id: range2108911
version: 1
direction: direction/forward
top level: 77RBS
start: 878
end: 918
orientation: inline

BBa_J23100
type: SequenceAnnotation
persistent identity: annotation2108905
display id: annotation2108905
version: 1
top level: 77RBS
location: https://synbiohub.org/public/igem/BBa_K356102/annotation2108905/range2108905/1
component: BBa_J23100

https://synbiohub.org/public/igem/BBa_K356102/annotation2108905/range2108905/1
type: Range
persistent identity: range2108905
display id: range2108905
version: 1
direction: direction/reverse
top level: 77RBS
start: 1
end: 35
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation2108908
display id: annotation2108908
version: 1
top level: 77RBS
location: https://synbiohub.org/public/igem/BBa_K356102/annotation2108908/range2108908/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_K356102/annotation2108908/range2108908/1
type: Range
persistent identity: range2108908
display id: range2108908
version: 1
direction: direction/reverse
top level: 77RBS
start: 62
end: 781
orientation: inline

BBa_K1031114
type: ComponentDefinition
persistent identity: BBa K1031114
display id: BBa_K1031114
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Po-B0032-sfGFP (CapR)
creator: He Shuaixin
created: 2013-09-08T11:00:00Z
modified: 2015-05-08T01:08:47Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1338_
m user id: 0
owner id: 15843
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Po-32-sfGFP (CapR)
mutable notes: molecular cloning
mutable provenance: synthesis, lab made
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: B0032
sequence annotation: Po (CapR)
sequence annotation: sfGFP
sequence annotation: B0015
sequence: https://synbiohub.org/public/igem/BBa_K1031114_sequence/1

B0032
type: SequenceAnnotation
persistent identity: annotation2362069
display id: annotation2362069
version: 1
top level: BBa_K1031114
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1031114/annotation2362069/range2362069/1

https://synbiohub.org/public/igem/BBa_K1031114/annotation2362069/range2362069/1
type: Range
persistent identity: range2362069
display id: range2362069
version: 1
direction: direction/forward
top level: BBa_K1031114
start: 177
end: 189
orientation: inline

Po (CapR)
type: SequenceAnnotation
persistent identity: annotation2362068
display id: annotation2362068
version: 1
top level: BBa_K1031114
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1031114/annotation2362068/range2362068/1

https://synbiohub.org/public/igem/BBa_K1031114/annotation2362068/range2362068/1
type: Range
persistent identity: range2362068
display id: range2362068
version: 1
direction: direction/forward
top level: BBa_K1031114
start: 1
end: 170
orientation: inline

sfGFP
type: SequenceAnnotation
persistent identity: annotation2362070
display id: annotation2362070
version: 1
top level: BBa_K1031114
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1031114/annotation2362070/range2362070/1

https://synbiohub.org/public/igem/BBa_K1031114/annotation2362070/range2362070/1
type: Range
persistent identity: range2362070
display id: range2362070
version: 1
direction: direction/forward
top level: BBa_K1031114
start: 196
end: 918
orientation: inline

B0015
type: SequenceAnnotation
persistent identity: annotation2362071
display id: annotation2362071
version: 1
top level: BBa_K1031114
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1031114/annotation2362071/range2362071/1

https://synbiohub.org/public/igem/BBa_K1031114/annotation2362071/range2362071/1
type: Range
persistent identity: range2362071
display id: range2362071
version: 1
direction: direction/forward
top level: BBa_K1031114
start: 925
end: 1053
orientation: inline

BBa_K1031301
type: ComponentDefinition
persistent identity: BBa K1031301
display id: BBa_K1031301
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Pc-B0031-sfGFP-Terminator (HbpR)
creator: He Shuaixin
created: 2013-09-08T11:00:00Z
modified: 2015-05-08T01:08:47Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1338_
m user id: 0
owner id: 15843
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Ph-31-sfGFP-TT (HbpR)
mutable notes: molecular cloning
mutable provenance: synthesis, lab made
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: BBa_B0031
sequence annotation: sfGFP
sequence annotation: BBa_B0015
sequence annotation: Ph
sequence: https://synbiohub.org/public/igem/BBa_K1031301_sequence/1

BBa_B0031
type: SequenceAnnotation
persistent identity: annotation2360427
display id: annotation2360427
version: 1
top level: BBa_K1031301
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1031301/annotation2360427/range2360427/1

https://synbiohub.org/public/igem/BBa_K1031301/annotation2360427/range2360427/1
type: Range
persistent identity: range2360427
display id: range2360427
version: 1
direction: direction/forward
top level: BBa_K1031301
start: 236
end: 248
orientation: inline

sfGFP
type: SequenceAnnotation
persistent identity: annotation2360428
display id: annotation2360428
version: 1
top level: BBa_K1031301
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1031301/annotation2360428/range2360428/1

https://synbiohub.org/public/igem/BBa_K1031301/annotation2360428/range2360428/1
type: Range
persistent identity: range2360428
display id: range2360428
version: 1
direction: direction/forward
top level: BBa_K1031301
start: 255
end: 977
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2360429
display id: annotation2360429
version: 1
top level: BBa_K1031301
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1031301/annotation2360429/range2360429/1

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type: Range
persistent identity: range2360429
display id: range2360429
version: 1
direction: direction/forward
top level: BBa_K1031301
start: 984
end: 1112
orientation: inline

Ph
type: SequenceAnnotation
persistent identity: annotation2360426
display id: annotation2360426
version: 1
top level: BBa_K1031301
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1031301/annotation2360426/range2360426/1

https://synbiohub.org/public/igem/BBa_K1031301/annotation2360426/range2360426/1
type: Range
persistent identity: range2360426
display id: range2360426
version: 1
direction: direction/forward
top level: BBa_K1031301
start: 1
end: 229
orientation: inline

BBa_K1031302
type: ComponentDefinition
persistent identity: BBa K1031302
display id: BBa_K1031302
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Pc-B0032-sfGFP-Terminator (HbpR)
creator: He Shuaixin
created: 2013-09-08T11:00:00Z
modified: 2015-05-08T01:08:47Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1338_
m user id: 0
owner id: 15843
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Ph-32-sfGFP-TT (HbpR)
mutable notes: molecular cloning
mutable provenance: synthesis, lab made
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: sfGFP
sequence annotation: BBa_B0015
sequence annotation: Ph
sequence annotation: BBa_B0032
sequence: https://synbiohub.org/public/igem/BBa_K1031302_sequence/1

sfGFP
type: SequenceAnnotation
persistent identity: annotation2360438
display id: annotation2360438
version: 1
top level: BBa_K1031302
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1031302/annotation2360438/range2360438/1

https://synbiohub.org/public/igem/BBa_K1031302/annotation2360438/range2360438/1
type: Range
persistent identity: range2360438
display id: range2360438
version: 1
direction: direction/forward
top level: BBa_K1031302
start: 254
end: 976
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2360439
display id: annotation2360439
version: 1
top level: BBa_K1031302
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1031302/annotation2360439/range2360439/1

https://synbiohub.org/public/igem/BBa_K1031302/annotation2360439/range2360439/1
type: Range
persistent identity: range2360439
display id: range2360439
version: 1
direction: direction/forward
top level: BBa_K1031302
start: 983
end: 1111
orientation: inline

Ph
type: SequenceAnnotation
persistent identity: annotation2360436
display id: annotation2360436
version: 1
top level: BBa_K1031302
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1031302/annotation2360436/range2360436/1

https://synbiohub.org/public/igem/BBa_K1031302/annotation2360436/range2360436/1
type: Range
persistent identity: range2360436
display id: range2360436
version: 1
direction: direction/forward
top level: BBa_K1031302
start: 1
end: 229
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation2360437
display id: annotation2360437
version: 1
top level: BBa_K1031302
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1031302/annotation2360437/range2360437/1

https://synbiohub.org/public/igem/BBa_K1031302/annotation2360437/range2360437/1
type: Range
persistent identity: range2360437
display id: range2360437
version: 1
direction: direction/forward
top level: BBa_K1031302
start: 236
end: 247
orientation: inline

BBa_K1732000
type: ComponentDefinition
persistent identity: BBa K1732000
display id: BBa_K1732000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: J23100-PelB-GlucHCO-B0015
creator: Donna Lee
created: 2015-09-02T11:00:00Z
modified: 2015-09-18T01:01:25Z
discontinued: false
dominant: false
group u list: _2154_
m user id: 27195
owner id: 27195
owning group id: 9
status: status/Planning
bookmark: false
mutable description: J23100 Promoter with a RBS (BBa_S04423), PelB Leader sequence, Gaussia princeps luciferase, and a B0015 terminator. Allows Gaussia princeps luciferase to be transcribed and translated using a strong promoter. Luminescence can be expressed by reacting the luciferase product with coelenterazine.
mutable notes: This sequence is codon optimized for E.coli. Sequence contains a PelB leader sequence that allows the Gaussia princeps luciferase proteins to secreted into the periplasmic space of the cell.
mutable provenance: Engineered from XXXXXXXX. Provided by the Bruchez lab at Carnegie Mellon.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/DNA
role: SO:0000110
component: BBa_B0034
component: BBa_B0015
component: BBa_K208004
component: BBa_J23100
component: BBa_K1732027
sequence annotation: BBa_J23100
sequence annotation: BBa_K1732027
sequence annotation: BBa_B0034
sequence annotation: BBa_B0015
sequence annotation: BBa_K208004
sequence: https://synbiohub.org/public/igem/BBa_K1732000_sequence/1

BBa_B0034
type: Component
persistent identity: component2469777
display id: component2469777
version: 1
top level: BBa_K1732000
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0015
type: Component
persistent identity: component2469790
display id: component2469790
version: 1
top level: BBa_K1732000
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_K208004
type: Component
persistent identity: component2469780
display id: component2469780
version: 1
top level: BBa_K1732000
access: public
definition: https://synbiohub.org/public/igem/BBa_K208004/1

BBa_J23100
type: Component
persistent identity: component2469775
display id: component2469775
version: 1
top level: BBa_K1732000
access: public
definition: https://synbiohub.org/public/igem/BBa_J23100/1

BBa_K1732027
type: Component
persistent identity: component2469783
display id: component2469783
version: 1
top level: BBa_K1732000
access: public
definition: https://synbiohub.org/public/igem/BBa_K1732027/1

BBa_J23100
type: SequenceAnnotation
persistent identity: annotation2469775
display id: annotation2469775
version: 1
top level: BBa_K1732000
location: https://synbiohub.org/public/igem/BBa_K1732000/annotation2469775/range2469775/1
component: BBa_J23100

https://synbiohub.org/public/igem/BBa_K1732000/annotation2469775/range2469775/1
type: Range
persistent identity: range2469775
display id: range2469775
version: 1
direction: direction/reverse
top level: BBa_K1732000
start: 1
end: 35
orientation: inline

BBa_K1732027
type: SequenceAnnotation
persistent identity: annotation2469783
display id: annotation2469783
version: 1
top level: BBa_K1732000
location: https://synbiohub.org/public/igem/BBa_K1732000/annotation2469783/range2469783/1
component: BBa_K1732027

https://synbiohub.org/public/igem/BBa_K1732000/annotation2469783/range2469783/1
type: Range
persistent identity: range2469783
display id: range2469783
version: 1
direction: direction/reverse
top level: BBa_K1732000
start: 136
end: 657
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2469777
display id: annotation2469777
version: 1
top level: BBa_K1732000
location: https://synbiohub.org/public/igem/BBa_K1732000/annotation2469777/range2469777/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1732000/annotation2469777/range2469777/1
type: Range
persistent identity: range2469777
display id: range2469777
version: 1
direction: direction/reverse
top level: BBa_K1732000
start: 44
end: 55
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2469790
display id: annotation2469790
version: 1
top level: BBa_K1732000
location: https://synbiohub.org/public/igem/BBa_K1732000/annotation2469790/range2469790/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K1732000/annotation2469790/range2469790/1
type: Range
persistent identity: range2469790
display id: range2469790
version: 1
direction: direction/reverse
top level: BBa_K1732000
start: 666
end: 794
orientation: inline

BBa_K208004
type: SequenceAnnotation
persistent identity: annotation2469780
display id: annotation2469780
version: 1
top level: BBa_K1732000
location: https://synbiohub.org/public/igem/BBa_K1732000/annotation2469780/range2469780/1
component: BBa_K208004

https://synbiohub.org/public/igem/BBa_K1732000/annotation2469780/range2469780/1
type: Range
persistent identity: range2469780
display id: range2469780
version: 1
direction: direction/reverse
top level: BBa_K1732000
start: 62
end: 127
orientation: inline