//rbs/prokaryote/constitutive/anderson
type: Collection
persistent identity: rbs prokaryote constitutive anderson
display id: rbs_prokaryote_constitutive_anderson
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
owned by: james
owned by: myers
member: BBa_J61134
member: BBa_K356101
member: BBa_J61139
member: BBa_J61109
member: BBa_J61104
member: BBa_J61113
member: BBa_K356106
member: BBa_J61127
member: BBa_J61106
member: BBa_K1468000
member: BBa_J61122
member: BBa_J61118
member: BBa_J61131
member: BBa_J61101
member: BBa_J61102
member: BBa_J61128
member: BBa_J61132
member: BBa_J61115
member: BBa_J61103
member: BBa_J61133
member: BBa_K1468002
member: BBa_J61120
member: BBa_J61116
member: BBa_J61129
member: BBa_K1468001
member: BBa_J61126
member: BBa_J61121
member: CBDclos(J6
member: BBa_J61117
member: BBa_J61130
member: BBa_J61100
member: BBa_J61135
member: BBa_J61105
member: BBa_K1709003
member: BBa_J61114
member: BBa_K259007
member: BBa_J61110
member: BBa_J61123
member: BBa_J61136
member: BBa_J61107
member: BBa_J61124
member: BBa_J61111
member: BBa_J61119
member: CBDcex(J61
member: BBa_J61108
member: BBa_J61138
member: BBa_J61112
member: BBa_J61137
member: BBa_J61125
member: BBa_K259013
member: BBa_K1709001

BBa_J61109
type: ComponentDefinition
persistent identity: BBa J61109
display id: BBa_J61109
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ribosome Binding Site Family Member
creator: John Anderson
created: 2007-04-23T11:00:00Z
modified: 2015-08-31T02:03:00Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable description: {{JCA_Arkin_RBSFamily}}
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J61109_sequence/1

CBDcex(J61
type: ComponentDefinition
persistent identity: BBa K863104
display id: BBa_K863104
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Cellulose binding Domain from C. Fimi with const. Promotor and GFP
creator: Moritz M??ller
created: 2012-09-20T11:00:00Z
modified: 2015-06-17T01:19:39Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1123_
m user id: 4206
owner id: 13080
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Cellulose binding Domain from Cellulomonas Fimi with const. Promotor J61101 and Reporter GFP
mutable notes: a switch to a const. Promoter
mutable provenance: different
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: BBa_J61101
sequence annotation: Exoglucanase
sequence annotation: AgeI
sequence annotation: His-Tag
sequence annotation: NgoMIV
sequence annotation: Freiburg-Scar
sequence annotation: Cellulose binding domain (CBDcex)
sequence annotation: BBa_E0040
sequence annotation: BBa_K863121
sequence annotation: BBa_J23100
sequence annotation: BBa_K863101
sequence annotation: GS-Linker
sequence annotation: ATG
sequence annotation: TAA
sequence: https://synbiohub.org/public/igem/BBa_K863104_sequence/1

BBa_J61101
type: SequenceAnnotation
persistent identity: annotation2189484
display id: annotation2189484
version: 1
top level: CBDcex(J61
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K863104/annotation2189484/range2189484/1

https://synbiohub.org/public/igem/BBa_K863104/annotation2189484/range2189484/1
type: Range
persistent identity: range2189484
display id: range2189484
version: 1
direction: direction/forward
top level: CBDcex(J61
start: 44
end: 55
orientation: inline

Exoglucanase
type: SequenceAnnotation
persistent identity: annotation2189488
display id: annotation2189488
version: 1
top level: CBDcex(J61
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K863104/annotation2189488/range2189488/1

https://synbiohub.org/public/igem/BBa_K863104/annotation2189488/range2189488/1
type: Range
persistent identity: range2189488
display id: range2189488
version: 1
direction: direction/forward
top level: CBDcex(J61
start: 71
end: 82
orientation: inline

AgeI
type: SequenceAnnotation
persistent identity: annotation2189496
display id: annotation2189496
version: 1
top level: CBDcex(J61
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K863104/annotation2189496/range2189496/1

https://synbiohub.org/public/igem/BBa_K863104/annotation2189496/range2189496/1
type: Range
persistent identity: range2189496
display id: range2189496
version: 1
direction: direction/forward
top level: CBDcex(J61
start: 1136
end: 1141
orientation: inline

His-Tag
type: SequenceAnnotation
persistent identity: annotation2189495
display id: annotation2189495
version: 1
top level: CBDcex(J61
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K863104/annotation2189495/range2189495/1

https://synbiohub.org/public/igem/BBa_K863104/annotation2189495/range2189495/1
type: Range
persistent identity: range2189495
display id: range2189495
version: 1
direction: direction/forward
top level: CBDcex(J61
start: 1118
end: 1135
orientation: inline

NgoMIV
type: SequenceAnnotation
persistent identity: annotation2189487
display id: annotation2189487
version: 1
top level: CBDcex(J61
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K863104/annotation2189487/range2189487/1

https://synbiohub.org/public/igem/BBa_K863104/annotation2189487/range2189487/1
type: Range
persistent identity: range2189487
display id: range2189487
version: 1
direction: direction/forward
top level: CBDcex(J61
start: 65
end: 70
orientation: inline

Freiburg-Scar
type: SequenceAnnotation
persistent identity: annotation2189492
display id: annotation2189492
version: 1
top level: CBDcex(J61
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K863104/annotation2189492/range2189492/1

https://synbiohub.org/public/igem/BBa_K863104/annotation2189492/range2189492/1
type: Range
persistent identity: range2189492
display id: range2189492
version: 1
direction: direction/forward
top level: CBDcex(J61
start: 398
end: 403
orientation: inline

Cellulose binding domain (CBDcex)
type: SequenceAnnotation
persistent identity: annotation2189489
display id: annotation2189489
version: 1
top level: CBDcex(J61
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K863104/annotation2189489/range2189489/1

https://synbiohub.org/public/igem/BBa_K863104/annotation2189489/range2189489/1
type: Range
persistent identity: range2189489
display id: range2189489
version: 1
direction: direction/forward
top level: CBDcex(J61
start: 83
end: 382
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation2189494
display id: annotation2189494
version: 1
top level: CBDcex(J61
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K863104/annotation2189494/range2189494/1

https://synbiohub.org/public/igem/BBa_K863104/annotation2189494/range2189494/1
type: Range
persistent identity: range2189494
display id: range2189494
version: 1
direction: direction/forward
top level: CBDcex(J61
start: 404
end: 1117
orientation: inline

BBa_K863121
type: SequenceAnnotation
persistent identity: annotation2189493
display id: annotation2189493
version: 1
top level: CBDcex(J61
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K863104/annotation2189493/range2189493/1

https://synbiohub.org/public/igem/BBa_K863104/annotation2189493/range2189493/1
type: Range
persistent identity: range2189493
display id: range2189493
version: 1
direction: direction/forward
top level: CBDcex(J61
start: 404
end: 1144
orientation: inline

BBa_J23100
type: SequenceAnnotation
persistent identity: annotation2189483
display id: annotation2189483
version: 1
top level: CBDcex(J61
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K863104/annotation2189483/range2189483/1

https://synbiohub.org/public/igem/BBa_K863104/annotation2189483/range2189483/1
type: Range
persistent identity: range2189483
display id: range2189483
version: 1
direction: direction/forward
top level: CBDcex(J61
start: 1
end: 35
orientation: inline

Exoglucanase
type: SequenceAnnotation
persistent identity: annotation2189490
display id: annotation2189490
version: 1
top level: CBDcex(J61
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K863104/annotation2189490/range2189490/1

https://synbiohub.org/public/igem/BBa_K863104/annotation2189490/range2189490/1
type: Range
persistent identity: range2189490
display id: range2189490
version: 1
direction: direction/forward
top level: CBDcex(J61
start: 383
end: 391
orientation: inline

BBa_K863101
type: SequenceAnnotation
persistent identity: annotation2189486
display id: annotation2189486
version: 1
top level: CBDcex(J61
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K863104/annotation2189486/range2189486/1

https://synbiohub.org/public/igem/BBa_K863104/annotation2189486/range2189486/1
type: Range
persistent identity: range2189486
display id: range2189486
version: 1
direction: direction/forward
top level: CBDcex(J61
start: 62
end: 397
orientation: inline

GS-Linker
type: SequenceAnnotation
persistent identity: annotation2189491
display id: annotation2189491
version: 1
top level: CBDcex(J61
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K863104/annotation2189491/range2189491/1

https://synbiohub.org/public/igem/BBa_K863104/annotation2189491/range2189491/1
type: Range
persistent identity: range2189491
display id: range2189491
version: 1
direction: direction/forward
top level: CBDcex(J61
start: 392
end: 397
orientation: inline

ATG
type: SequenceAnnotation
persistent identity: annotation2189485
display id: annotation2189485
version: 1
top level: CBDcex(J61
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K863104/annotation2189485/range2189485/1

https://synbiohub.org/public/igem/BBa_K863104/annotation2189485/range2189485/1
type: Range
persistent identity: range2189485
display id: range2189485
version: 1
direction: direction/forward
top level: CBDcex(J61
start: 62
end: 64
orientation: inline

TAA
type: SequenceAnnotation
persistent identity: annotation2189497
display id: annotation2189497
version: 1
top level: CBDcex(J61
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K863104/annotation2189497/range2189497/1

https://synbiohub.org/public/igem/BBa_K863104/annotation2189497/range2189497/1
type: Range
persistent identity: range2189497
display id: range2189497
version: 1
direction: direction/forward
top level: CBDcex(J61
start: 1142
end: 1144
orientation: inline

BBa_J61129
type: ComponentDefinition
persistent identity: BBa J61129
display id: BBa_J61129
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ribosome Binding Site Family Member
creator: John Anderson
created: 2007-04-24T11:00:00Z
modified: 2015-08-31T01:59:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable description: {{JCA_Arkin_RBSFamily}}
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J61129_sequence/1

BBa_J61113
type: ComponentDefinition
persistent identity: BBa J61113
display id: BBa_J61113
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ribosome Binding Site Family Member
creator: John Anderson
created: 2007-04-23T11:00:00Z
modified: 2015-08-31T02:03:00Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable description: {{JCA_Arkin_RBSFamily}}
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J61113_sequence/1

BBa_J61118
type: ComponentDefinition
persistent identity: BBa J61118
display id: BBa_J61118
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ribosome Binding Site Family Member
creator: John Anderson
created: 2007-04-23T11:00:00Z
modified: 2015-08-31T01:59:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable description: {{JCA_Arkin_RBSFamily}}
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J61118_sequence/1

BBa_J61101
type: ComponentDefinition
persistent identity: BBa J61101
display id: BBa_J61101
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ribosome Binding Site Family Member
creator: John Anderson
created: 2007-01-28T12:00:00Z
modified: 2015-08-31T02:03:00Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable description: fix
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J61101_sequence/1

BBa_J61116
type: ComponentDefinition
persistent identity: BBa J61116
display id: BBa_J61116
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ribosome Binding Site Family Member
creator: John Anderson
created: 2007-04-23T11:00:00Z
modified: 2015-08-31T02:03:00Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable description: {{JCA_Arkin_RBSFamily}}
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J61116_sequence/1

BBa_J61126
type: ComponentDefinition
persistent identity: BBa J61126
display id: BBa_J61126
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ribosome Binding Site Family Member
creator: John Anderson
created: 2007-04-24T11:00:00Z
modified: 2015-08-31T01:59:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable description: {{JCA_Arkin_RBSFamily}}
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J61126_sequence/1

BBa_J61110
type: ComponentDefinition
persistent identity: BBa J61110
display id: BBa_J61110
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ribosome Binding Site Family Member
creator: John Anderson
created: 2007-04-23T11:00:00Z
modified: 2015-08-31T02:03:00Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable description: {{JCA_Arkin_RBSFamily}}
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J61110_sequence/1

BBa_K1709003
type: ComponentDefinition
persistent identity: BBa K1709003
display id: BBa_K1709003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CheZ-GFP
creator: Astrid Deryckere
created: 2015-09-15T11:00:00Z
modified: 2015-09-26T08:47:17Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2129_
m user id: 25389
owner id: 25389
owning group id: 9
status: status/Planning
bookmark: true
mutable description: Upon recognition of a small molecule (e.g. nutrients or toxins), chemoreceptors transduce a signal through a set of Che proteins. This group of methylesterases and phosphatases regulates the rotation of the flagella. At the end of the chain of Che proteins is CheY. In phosphorylated state, CheY binds the flagella and causes the cells to tumble. The phosphatase CheZ dephosphorylates CheY, inducing dissociation of CheY from the flagella enabling the cells to swim. Cells lacking the CheZ protein are unable to swim and will tumble excessively and incessantly.
mutable notes: 1 bp in BBa_K629003 was adapted to create an original restriciton site in the plasmid. CheZ was fused to GFP (BBa_K082003) and its LVA tag was codon-optimized to avoid introducing artificial DNA repetitions.
mutable provenance: DNA sequences originate from the E. Coli genome and previous biobrick sequences. The ribosome binding site was chosen from the Anderson RBS family.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K1709002
component: BBa_J61101
sequence annotation: BBa_J61101
sequence annotation: BBa_K1709002
sequence: https://synbiohub.org/public/igem/BBa_K1709003_sequence/1

BBa_K1709002
type: Component
persistent identity: component2472552
display id: component2472552
version: 1
top level: BBa_K1709003
access: public
definition: https://synbiohub.org/public/igem/BBa_K1709002/1

BBa_J61101
type: Component
persistent identity: component2472546
display id: component2472546
version: 1
top level: BBa_K1709003
access: public
definition: BBa_J61101

BBa_J61101
type: SequenceAnnotation
persistent identity: annotation2472546
display id: annotation2472546
version: 1
top level: BBa_K1709003
location: https://synbiohub.org/public/igem/BBa_K1709003/annotation2472546/range2472546/1
component: BBa_J61101

https://synbiohub.org/public/igem/BBa_K1709003/annotation2472546/range2472546/1
type: Range
persistent identity: range2472546
display id: range2472546
version: 1
direction: direction/reverse
top level: BBa_K1709003
start: 1
end: 12
orientation: inline

BBa_K1709002
type: SequenceAnnotation
persistent identity: annotation2472552
display id: annotation2472552
version: 1
top level: BBa_K1709003
location: https://synbiohub.org/public/igem/BBa_K1709003/annotation2472552/range2472552/1
component: BBa_K1709002

https://synbiohub.org/public/igem/BBa_K1709003/annotation2472552/range2472552/1
type: Range
persistent identity: range2472552
display id: range2472552
version: 1
direction: direction/reverse
top level: BBa_K1709003
start: 19
end: 1419
orientation: inline

BBa_J61100
type: ComponentDefinition
persistent identity: BBa J61100
display id: BBa_J61100
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ribosome Binding Site Family Member
creator: John Anderson
created: 2007-01-28T12:00:00Z
modified: 2015-08-31T02:03:00Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable description: {{JCA_Arkin_RBSFamily}}
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J61100_sequence/1

BBa_J61137
type: ComponentDefinition
persistent identity: BBa J61137
display id: BBa_J61137
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ribosome Binding Site Family Member
creator: John Anderson
created: 2007-04-24T11:00:00Z
modified: 2015-08-31T01:59:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable description: {{JCA_Arkin_RBSFamily}}
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J61137_sequence/1

BBa_J61123
type: ComponentDefinition
persistent identity: BBa J61123
display id: BBa_J61123
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ribosome Binding Site Family Member
creator: John Anderson
created: 2007-04-24T11:00:00Z
modified: 2015-08-31T01:59:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable description: {{JCA_Arkin_RBSFamily}}
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J61123_sequence/1

BBa_J61132
type: ComponentDefinition
persistent identity: BBa J61132
display id: BBa_J61132
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ribosome Binding Site Family Member
creator: John Anderson
created: 2007-04-24T11:00:00Z
modified: 2015-08-31T01:59:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable description: {{JCA_Arkin_RBSFamily}}
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J61132_sequence/1

BBa_J61103
type: ComponentDefinition
persistent identity: BBa J61103
display id: BBa_J61103
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ribosome Binding Site Family Member
creator: John Anderson
created: 2007-04-23T11:00:00Z
modified: 2015-08-31T02:03:00Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable description: {{JCA_Arkin_RBSFamily}}
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J61103_sequence/1

BBa_J61134
type: ComponentDefinition
persistent identity: BBa J61134
display id: BBa_J61134
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ribosome Binding Site Family Member
creator: John Anderson
created: 2007-04-24T11:00:00Z
modified: 2015-08-31T01:59:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable description: {{JCA_Arkin_RBSFamily}}
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J61134_sequence/1

BBa_J61119
type: ComponentDefinition
persistent identity: BBa J61119
display id: BBa_J61119
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ribosome Binding Site Family Member
creator: John Anderson
created: 2007-04-24T11:00:00Z
modified: 2015-08-31T01:59:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable description: {{JCA_Arkin_RBSFamily}}
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J61119_sequence/1

BBa_J61106
type: ComponentDefinition
persistent identity: BBa J61106
display id: BBa_J61106
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ribosome Binding Site Family Member
creator: John Anderson
created: 2007-04-23T11:00:00Z
modified: 2015-08-31T02:03:00Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable description: {{JCA_Arkin_RBSFamily}}
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J61106_sequence/1

BBa_J61121
type: ComponentDefinition
persistent identity: BBa J61121
display id: BBa_J61121
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ribosome Binding Site Family Member
creator: John Anderson
created: 2007-04-24T11:00:00Z
modified: 2015-08-31T01:59:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable description: {{JCA_Arkin_RBSFamily}}
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J61121_sequence/1

BBa_J61138
type: ComponentDefinition
persistent identity: BBa J61138
display id: BBa_J61138
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ribosome Binding Site Family Member
creator: John Anderson
created: 2007-04-24T11:00:00Z
modified: 2015-08-31T01:59:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable description: {{JCA_Arkin_RBSFamily}}
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J61138_sequence/1

BBa_K1468000
type: ComponentDefinition
persistent identity: BBa K1468000
display id: BBa_K1468000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pJ23104 + gene encoding ZsGreen
creator: Pedro Luis Dorado Morales
created: 2014-10-02T11:00:00Z
modified: 2015-05-08T01:10:38Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1847_
m user id: 0
owner id: 11756
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: -
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: ZsGreen protein
sequence annotation: STOP
sequence annotation: BBa_K1468000
sequence annotation: BBa_J23104
sequence annotation: START
sequence annotation: ZsGreeen
sequence annotation: J23104p
sequence annotation: BBa_E0040
sequence: https://synbiohub.org/public/igem/BBa_K1468000_sequence/1

ZsGreen protein
type: SequenceAnnotation
persistent identity: annotation2418059
display id: annotation2418059
version: 1
top level: BBa_K1468000
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1468000/annotation2418059/range2418059/1

https://synbiohub.org/public/igem/BBa_K1468000/annotation2418059/range2418059/1
type: Range
persistent identity: range2418059
display id: range2418059
version: 1
direction: direction/forward
top level: BBa_K1468000
start: 34
end: 725
orientation: inline

STOP
type: SequenceAnnotation
persistent identity: annotation2418064
display id: annotation2418064
version: 1
top level: BBa_K1468000
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1468000/annotation2418064/range2418064/1

https://synbiohub.org/public/igem/BBa_K1468000/annotation2418064/range2418064/1
type: Range
persistent identity: range2418064
display id: range2418064
version: 1
direction: direction/forward
top level: BBa_K1468000
start: 725
end: 725
orientation: inline

BBa_K1468000
type: SequenceAnnotation
persistent identity: annotation2418810
display id: annotation2418810
version: 1
top level: BBa_K1468000
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1468000/annotation2418810/range2418810/1

https://synbiohub.org/public/igem/BBa_K1468000/annotation2418810/range2418810/1
type: Range
persistent identity: range2418810
display id: range2418810
version: 1
direction: direction/forward
top level: BBa_K1468000
start: 1
end: 725
orientation: inline

BBa_J23104
type: SequenceAnnotation
persistent identity: annotation2418058
display id: annotation2418058
version: 1
top level: BBa_K1468000
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1468000/annotation2418058/range2418058/1

https://synbiohub.org/public/igem/BBa_K1468000/annotation2418058/range2418058/1
type: Range
persistent identity: range2418058
display id: range2418058
version: 1
direction: direction/forward
top level: BBa_K1468000
start: 1
end: 33
orientation: inline

START
type: SequenceAnnotation
persistent identity: annotation2418061
display id: annotation2418061
version: 1
top level: BBa_K1468000
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1468000/annotation2418061/range2418061/1

https://synbiohub.org/public/igem/BBa_K1468000/annotation2418061/range2418061/1
type: Range
persistent identity: range2418061
display id: range2418061
version: 1
direction: direction/forward
top level: BBa_K1468000
start: 34
end: 36
orientation: inline

ZsGreeen
type: SequenceAnnotation
persistent identity: annotation2418062
display id: annotation2418062
version: 1
top level: BBa_K1468000
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1468000/annotation2418062/range2418062/1

https://synbiohub.org/public/igem/BBa_K1468000/annotation2418062/range2418062/1
type: Range
persistent identity: range2418062
display id: range2418062
version: 1
direction: direction/forward
top level: BBa_K1468000
start: 34
end: 725
orientation: inline

J23104p
type: SequenceAnnotation
persistent identity: annotation2418030
display id: annotation2418030
version: 1
top level: BBa_K1468000
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1468000/annotation2418030/range2418030/1

https://synbiohub.org/public/igem/BBa_K1468000/annotation2418030/range2418030/1
type: Range
persistent identity: range2418030
display id: range2418030
version: 1
direction: direction/forward
top level: BBa_K1468000
start: 1
end: 33
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation2418060
display id: annotation2418060
version: 1
top level: BBa_K1468000
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1468000/annotation2418060/range2418060/1

https://synbiohub.org/public/igem/BBa_K1468000/annotation2418060/range2418060/1
type: Range
persistent identity: range2418060
display id: range2418060
version: 1
direction: direction/forward
top level: BBa_K1468000
start: 34
end: 725
orientation: inline

BBa_J61114
type: ComponentDefinition
persistent identity: BBa J61114
display id: BBa_J61114
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ribosome Binding Site Family Member
creator: John Anderson
created: 2007-04-23T11:00:00Z
modified: 2015-08-31T02:03:00Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable description: {{JCA_Arkin_RBSFamily}}
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J61114_sequence/1

BBa_K259013
type: ComponentDefinition
persistent identity: BBa K259013
display id: BBa_K259013
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: AraC-RBS-GFP-Terminator
creator: Petros Mina
created: 2009-10-14T11:00:00Z
modified: 2015-05-08T01:11:42Z
discontinued: false
dominant: false
group u list: _357_
m user id: 0
owner id: 5318
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: A part used to quantify the AraC-RBS construct <partinfo>K259007</partinfo> created by the BCCS-Bristol iGEM'09 team.
mutable notes: RFC10 assembly
mutable provenance: This part was designed with the specified biobricks from the parts registry. AraC occurs naturally in E.coli. GFP occurs in jellyfish species, RBS designed by the Anderson family and Terminator by Resma Shetty.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_R0080
component: BBa_J61100
component: BBa_B0012
component: BBa_B0011
component: BBa_R0081
component: BBa_E0040
sequence annotation: BBa_B0011
sequence annotation: BBa_R0081
sequence annotation: BBa_B0012
sequence annotation: BBa_R0080
sequence annotation: BBa_E0040
sequence annotation: BBa_J61100
sequence: https://synbiohub.org/public/igem/BBa_K259013_sequence/1

BBa_R0080
type: Component
persistent identity: component2041443
display id: component2041443
version: 1
top level: BBa_K259013
access: public
definition: https://synbiohub.org/public/igem/BBa_R0080/1

BBa_J61100
type: Component
persistent identity: component2041444
display id: component2041444
version: 1
top level: BBa_K259013
access: public
definition: BBa_J61100

BBa_B0012
type: Component
persistent identity: component2041447
display id: component2041447
version: 1
top level: BBa_K259013
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0011
type: Component
persistent identity: component2041451
display id: component2041451
version: 1
top level: BBa_K259013
access: public
definition: https://synbiohub.org/public/igem/BBa_B0011/1

BBa_R0081
type: Component
persistent identity: component2041436
display id: component2041436
version: 1
top level: BBa_K259013
access: public
definition: https://synbiohub.org/public/igem/BBa_R0081/1

BBa_E0040
type: Component
persistent identity: component2041446
display id: component2041446
version: 1
top level: BBa_K259013
access: public
definition: https://synbiohub.org/public/igem/BBa_E0040/1

BBa_B0011
type: SequenceAnnotation
persistent identity: annotation2041451
display id: annotation2041451
version: 1
top level: BBa_K259013
location: https://synbiohub.org/public/igem/BBa_K259013/annotation2041451/range2041451/1
component: BBa_B0011

https://synbiohub.org/public/igem/BBa_K259013/annotation2041451/range2041451/1
type: Range
persistent identity: range2041451
display id: range2041451
version: 1
direction: direction/forward
top level: BBa_K259013
start: 1144
end: 1189
orientation: inline

BBa_R0081
type: SequenceAnnotation
persistent identity: annotation2041436
display id: annotation2041436
version: 1
top level: BBa_K259013
location: https://synbiohub.org/public/igem/BBa_K259013/annotation2041436/range2041436/1
component: BBa_R0081

https://synbiohub.org/public/igem/BBa_K259013/annotation2041436/range2041436/1
type: Range
persistent identity: range2041436
display id: range2041436
version: 1
direction: direction/reverse
top level: BBa_K259013
start: 1
end: 183
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2041447
display id: annotation2041447
version: 1
top level: BBa_K259013
location: https://synbiohub.org/public/igem/BBa_K259013/annotation2041447/range2041447/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K259013/annotation2041447/range2041447/1
type: Range
persistent identity: range2041447
display id: range2041447
version: 1
direction: direction/forward
top level: BBa_K259013
start: 1095
end: 1135
orientation: inline

BBa_R0080
type: SequenceAnnotation
persistent identity: annotation2041443
display id: annotation2041443
version: 1
top level: BBa_K259013
location: https://synbiohub.org/public/igem/BBa_K259013/annotation2041443/range2041443/1
component: BBa_R0080

https://synbiohub.org/public/igem/BBa_K259013/annotation2041443/range2041443/1
type: Range
persistent identity: range2041443
display id: range2041443
version: 1
direction: direction/reverse
top level: BBa_K259013
start: 192
end: 340
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation2041446
display id: annotation2041446
version: 1
top level: BBa_K259013
location: https://synbiohub.org/public/igem/BBa_K259013/annotation2041446/range2041446/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_K259013/annotation2041446/range2041446/1
type: Range
persistent identity: range2041446
display id: range2041446
version: 1
direction: direction/reverse
top level: BBa_K259013
start: 367
end: 1086
orientation: inline

BBa_J61100
type: SequenceAnnotation
persistent identity: annotation2041444
display id: annotation2041444
version: 1
top level: BBa_K259013
location: https://synbiohub.org/public/igem/BBa_K259013/annotation2041444/range2041444/1
component: BBa_J61100

https://synbiohub.org/public/igem/BBa_K259013/annotation2041444/range2041444/1
type: Range
persistent identity: range2041444
display id: range2041444
version: 1
direction: direction/reverse
top level: BBa_K259013
start: 349
end: 360
orientation: inline

BBa_J61111
type: ComponentDefinition
persistent identity: BBa J61111
display id: BBa_J61111
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ribosome Binding Site Family Member
creator: John Anderson
created: 2007-04-23T11:00:00Z
modified: 2015-08-31T02:03:00Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable description: {{JCA_Arkin_RBSFamily}}
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J61111_sequence/1

BBa_J61124
type: ComponentDefinition
persistent identity: BBa J61124
display id: BBa_J61124
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ribosome Binding Site Family Member
creator: John Anderson
created: 2007-04-24T11:00:00Z
modified: 2015-08-31T01:59:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable description: {{JCA_Arkin_RBSFamily}}
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J61124_sequence/1

BBa_J61107
type: ComponentDefinition
persistent identity: BBa J61107
display id: BBa_J61107
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ribosome Binding Site Family Member
creator: John Anderson
created: 2007-04-23T11:00:00Z
modified: 2015-08-31T02:03:00Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable description: {{JCA_Arkin_RBSFamily}}
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J61107_sequence/1

BBa_J61131
type: ComponentDefinition
persistent identity: BBa J61131
display id: BBa_J61131
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ribosome Binding Site Family Member
creator: John Anderson
created: 2007-04-24T11:00:00Z
modified: 2015-08-31T01:59:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable description: {{JCA_Arkin_RBSFamily}}
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J61131_sequence/1

BBa_K1468002
type: ComponentDefinition
persistent identity: BBa K1468002
display id: BBa_K1468002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pJ23110 + gene encoding ZsGreen
creator: Pedro Luis Dorado Morales
created: 2014-10-02T11:00:00Z
modified: 2015-05-08T01:10:38Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1847_
m user id: 0
owner id: 11756
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: -
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence: https://synbiohub.org/public/igem/BBa_K1468002_sequence/1

BBa_K259007
type: ComponentDefinition
persistent identity: BBa K259007
display id: BBa_K259007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: AraC Promoter fused with RBS
creator: Petros Mina
created: 2009-09-08T11:00:00Z
modified: 2015-05-08T01:11:42Z
discontinued: false
dominant: false
group u list: _357_
m user id: 0
owner id: 5318
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part has the arabinose regulated promoter (AraC/pBAD) upstream of a ribosome binding site. This is ideal for inserting a protein to be expressed downstream of this part as it's expression can be regulated by the presence of the concentration of arabinose in the growing environment/media of the cells. ==What this part actually does== This composite part includes the AraC/pBAD promoter and a strong ribosome binding site designed from the Anderson family of RBS. The AraC/pBAD promoter can be used with prokaryotic chassis to regulate expresion of proteins. When the AraC/pBAD promoter is placed upstream of your canditate translational unit (protein to be expressed) it allows control of the trancription of the said protein. This is done via controlling the concentration of arabinose sugar in the environment/solution of bacterial growth. Controlling the transcription will not ensure translation. The ribosome binding site is ideally placed a small distance away from the promoter site to ensure that the ribosome will bind and start translating the protein coding sequence found downstream.
mutable notes: The part was designed with RFC10 assembly from supplied biobricks of the iGEM2009 distribution.
mutable provenance: The AraC promoter can be found naturally at 1.52min of the E.coli genome ([http://ecogene.org/geneinfo.php?eg_id=EG10054 EcoGene number EG10054]. The RBS was taken from the designed sequences of RBS by the Anderson family. For more information visit the original part page [http://partsregistry.org/Part:BBa_J61100 here].
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
component: BBa_R0081
component: BBa_R0080
component: BBa_J61100
sequence annotation: BBa_R0081
sequence annotation: BBa_J61100
sequence annotation: BBa_R0080
sequence: https://synbiohub.org/public/igem/BBa_K259007_sequence/1

BBa_R0081
type: Component
persistent identity: component2020539
display id: component2020539
version: 1
top level: BBa_K259007
access: public
definition: https://synbiohub.org/public/igem/BBa_R0081/1

BBa_R0080
type: Component
persistent identity: component2020546
display id: component2020546
version: 1
top level: BBa_K259007
access: public
definition: https://synbiohub.org/public/igem/BBa_R0080/1

BBa_J61100
type: Component
persistent identity: component2020547
display id: component2020547
version: 1
top level: BBa_K259007
access: public
definition: BBa_J61100

BBa_R0081
type: SequenceAnnotation
persistent identity: annotation2020539
display id: annotation2020539
version: 1
top level: BBa_K259007
location: https://synbiohub.org/public/igem/BBa_K259007/annotation2020539/range2020539/1
component: BBa_R0081

https://synbiohub.org/public/igem/BBa_K259007/annotation2020539/range2020539/1
type: Range
persistent identity: range2020539
display id: range2020539
version: 1
direction: direction/reverse
top level: BBa_K259007
start: 1
end: 183
orientation: inline

BBa_J61100
type: SequenceAnnotation
persistent identity: annotation2020547
display id: annotation2020547
version: 1
top level: BBa_K259007
location: https://synbiohub.org/public/igem/BBa_K259007/annotation2020547/range2020547/1
component: BBa_J61100

https://synbiohub.org/public/igem/BBa_K259007/annotation2020547/range2020547/1
type: Range
persistent identity: range2020547
display id: range2020547
version: 1
direction: direction/reverse
top level: BBa_K259007
start: 349
end: 360
orientation: inline

BBa_R0080
type: SequenceAnnotation
persistent identity: annotation2020546
display id: annotation2020546
version: 1
top level: BBa_K259007
location: https://synbiohub.org/public/igem/BBa_K259007/annotation2020546/range2020546/1
component: BBa_R0080

https://synbiohub.org/public/igem/BBa_K259007/annotation2020546/range2020546/1
type: Range
persistent identity: range2020546
display id: range2020546
version: 1
direction: direction/reverse
top level: BBa_K259007
start: 192
end: 340
orientation: inline

BBa_J61115
type: ComponentDefinition
persistent identity: BBa J61115
display id: BBa_J61115
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ribosome Binding Site Family Member
creator: John Anderson
created: 2007-04-23T11:00:00Z
modified: 2015-08-31T02:03:00Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable description: {{JCA_Arkin_RBSFamily}}
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J61115_sequence/1

BBa_J61135
type: ComponentDefinition
persistent identity: BBa J61135
display id: BBa_J61135
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ribosome Binding Site Family Member
creator: John Anderson
created: 2007-04-24T11:00:00Z
modified: 2015-08-31T01:59:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: {{JCA_Arkin_RBSFamily}}
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J61135_sequence/1

BBa_J61128
type: ComponentDefinition
persistent identity: BBa J61128
display id: BBa_J61128
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ribosome Binding Site Family Member
creator: John Anderson
created: 2007-04-24T11:00:00Z
modified: 2015-08-31T01:59:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable description: {{JCA_Arkin_RBSFamily}}
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J61128_sequence/1

BBa_J61120
type: ComponentDefinition
persistent identity: BBa J61120
display id: BBa_J61120
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ribosome Binding Site Family Member
creator: John Anderson
created: 2007-04-24T11:00:00Z
modified: 2015-08-31T01:59:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable description: {{JCA_Arkin_RBSFamily}}
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J61120_sequence/1

BBa_J61104
type: ComponentDefinition
persistent identity: BBa J61104
display id: BBa_J61104
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ribosome Binding Site Family Member
creator: John Anderson
created: 2007-04-23T11:00:00Z
modified: 2015-08-31T02:03:00Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable description: {{JCA_Arkin_RBSFamily}}
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J61104_sequence/1

BBa_J61127
type: ComponentDefinition
persistent identity: BBa J61127
display id: BBa_J61127
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ribosome Binding Site Family Member
creator: John Anderson
created: 2007-04-24T11:00:00Z
modified: 2015-08-31T01:59:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable description: {{JCA_Arkin_RBSFamily}}
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J61127_sequence/1

CBDclos(J6
type: ComponentDefinition
persistent identity: BBa K863114
display id: BBa_K863114
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CBD of C. cellulovorans cellulose binding protein gene with const. Promoter (J23100+J61101) and GFP
creator: Moritz M??ller
created: 2012-09-22T11:00:00Z
modified: 2015-06-17T01:20:44Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1123_
m user id: 4206
owner id: 13080
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Cellulose binding Domain of Clostridium cellulovorans [http://www.ncbi.nlm.nih.gov/nuccore/M73817 cellulose binding protein gene (cbp A)] with a constitutive promoter and RBS (<partinfo>J61101</partinfo>) and a fused-GFP protein with His-tag.
mutable notes: Was made to switch from a T7-promoter to a constitutive promoter
mutable provenance: Cellulose binding domain form a cloning-vector. <partinfo>J61101</partinfo> and [http://partsregistry.org/Part:BBa_I13522 GFP] from the partsregistry.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: Cellulose binding domain (CBDclos)
sequence annotation: Freiburg-Scar
sequence annotation: BBa_K863121
sequence annotation: BBa_E0040
sequence annotation: TAA
sequence annotation: GS-Linker
sequence annotation: ATG
sequence annotation: AgeI
sequence annotation: BBa_K863111
sequence annotation: BBa_J61101
sequence annotation: His-Tag
sequence annotation: NgoMIV
sequence annotation: Cellulose Binding Protein
sequence annotation: BBa_J23100
sequence: https://synbiohub.org/public/igem/BBa_K863114_sequence/1

Cellulose binding domain (CBDclos)
type: SequenceAnnotation
persistent identity: annotation2194135
display id: annotation2194135
version: 1
top level: CBDclos(J6
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K863114/annotation2194135/range2194135/1

https://synbiohub.org/public/igem/BBa_K863114/annotation2194135/range2194135/1
type: Range
persistent identity: range2194135
display id: range2194135
version: 1
direction: direction/forward
top level: CBDclos(J6
start: 83
end: 358
orientation: inline

Freiburg-Scar
type: SequenceAnnotation
persistent identity: annotation2194138
display id: annotation2194138
version: 1
top level: CBDclos(J6
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K863114/annotation2194138/range2194138/1

https://synbiohub.org/public/igem/BBa_K863114/annotation2194138/range2194138/1
type: Range
persistent identity: range2194138
display id: range2194138
version: 1
direction: direction/forward
top level: CBDclos(J6
start: 371
end: 376
orientation: inline

BBa_K863121
type: SequenceAnnotation
persistent identity: annotation2194139
display id: annotation2194139
version: 1
top level: CBDclos(J6
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K863114/annotation2194139/range2194139/1

https://synbiohub.org/public/igem/BBa_K863114/annotation2194139/range2194139/1
type: Range
persistent identity: range2194139
display id: range2194139
version: 1
direction: direction/reverse
top level: CBDclos(J6
start: 377
end: 1117
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation2194140
display id: annotation2194140
version: 1
top level: CBDclos(J6
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K863114/annotation2194140/range2194140/1

https://synbiohub.org/public/igem/BBa_K863114/annotation2194140/range2194140/1
type: Range
persistent identity: range2194140
display id: range2194140
version: 1
direction: direction/forward
top level: CBDclos(J6
start: 377
end: 1090
orientation: inline

TAA
type: SequenceAnnotation
persistent identity: annotation2194143
display id: annotation2194143
version: 1
top level: CBDclos(J6
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K863114/annotation2194143/range2194143/1

https://synbiohub.org/public/igem/BBa_K863114/annotation2194143/range2194143/1
type: Range
persistent identity: range2194143
display id: range2194143
version: 1
direction: direction/forward
top level: CBDclos(J6
start: 1115
end: 1117
orientation: inline

GS-Linker
type: SequenceAnnotation
persistent identity: annotation2194137
display id: annotation2194137
version: 1
top level: CBDclos(J6
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K863114/annotation2194137/range2194137/1

https://synbiohub.org/public/igem/BBa_K863114/annotation2194137/range2194137/1
type: Range
persistent identity: range2194137
display id: range2194137
version: 1
direction: direction/forward
top level: CBDclos(J6
start: 365
end: 370
orientation: inline

ATG
type: SequenceAnnotation
persistent identity: annotation2194132
display id: annotation2194132
version: 1
top level: CBDclos(J6
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K863114/annotation2194132/range2194132/1

https://synbiohub.org/public/igem/BBa_K863114/annotation2194132/range2194132/1
type: Range
persistent identity: range2194132
display id: range2194132
version: 1
direction: direction/forward
top level: CBDclos(J6
start: 62
end: 64
orientation: inline

AgeI
type: SequenceAnnotation
persistent identity: annotation2194142
display id: annotation2194142
version: 1
top level: CBDclos(J6
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K863114/annotation2194142/range2194142/1

https://synbiohub.org/public/igem/BBa_K863114/annotation2194142/range2194142/1
type: Range
persistent identity: range2194142
display id: range2194142
version: 1
direction: direction/forward
top level: CBDclos(J6
start: 1109
end: 1114
orientation: inline

BBa_K863111
type: SequenceAnnotation
persistent identity: annotation2194131
display id: annotation2194131
version: 1
top level: CBDclos(J6
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K863114/annotation2194131/range2194131/1

https://synbiohub.org/public/igem/BBa_K863114/annotation2194131/range2194131/1
type: Range
persistent identity: range2194131
display id: range2194131
version: 1
direction: direction/reverse
top level: CBDclos(J6
start: 62
end: 370
orientation: inline

BBa_J61101
type: SequenceAnnotation
persistent identity: annotation2194130
display id: annotation2194130
version: 1
top level: CBDclos(J6
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K863114/annotation2194130/range2194130/1

https://synbiohub.org/public/igem/BBa_K863114/annotation2194130/range2194130/1
type: Range
persistent identity: range2194130
display id: range2194130
version: 1
direction: direction/reverse
top level: CBDclos(J6
start: 44
end: 55
orientation: inline

His-Tag
type: SequenceAnnotation
persistent identity: annotation2194141
display id: annotation2194141
version: 1
top level: CBDclos(J6
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K863114/annotation2194141/range2194141/1

https://synbiohub.org/public/igem/BBa_K863114/annotation2194141/range2194141/1
type: Range
persistent identity: range2194141
display id: range2194141
version: 1
direction: direction/forward
top level: CBDclos(J6
start: 1091
end: 1108
orientation: inline

NgoMIV
type: SequenceAnnotation
persistent identity: annotation2194133
display id: annotation2194133
version: 1
top level: CBDclos(J6
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K863114/annotation2194133/range2194133/1

https://synbiohub.org/public/igem/BBa_K863114/annotation2194133/range2194133/1
type: Range
persistent identity: range2194133
display id: range2194133
version: 1
direction: direction/forward
top level: CBDclos(J6
start: 65
end: 70
orientation: inline

Cellulose Binding Protein
type: SequenceAnnotation
persistent identity: annotation2194134
display id: annotation2194134
version: 1
top level: CBDclos(J6
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K863114/annotation2194134/range2194134/1

https://synbiohub.org/public/igem/BBa_K863114/annotation2194134/range2194134/1
type: Range
persistent identity: range2194134
display id: range2194134
version: 1
direction: direction/forward
top level: CBDclos(J6
start: 71
end: 82
orientation: inline

Cellulose Binding Protein
type: SequenceAnnotation
persistent identity: annotation2194136
display id: annotation2194136
version: 1
top level: CBDclos(J6
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K863114/annotation2194136/range2194136/1

https://synbiohub.org/public/igem/BBa_K863114/annotation2194136/range2194136/1
type: Range
persistent identity: range2194136
display id: range2194136
version: 1
direction: direction/forward
top level: CBDclos(J6
start: 359
end: 364
orientation: inline

BBa_J23100
type: SequenceAnnotation
persistent identity: annotation2194129
display id: annotation2194129
version: 1
top level: CBDclos(J6
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K863114/annotation2194129/range2194129/1

https://synbiohub.org/public/igem/BBa_K863114/annotation2194129/range2194129/1
type: Range
persistent identity: range2194129
display id: range2194129
version: 1
direction: direction/reverse
top level: CBDclos(J6
start: 1
end: 35
orientation: inline

BBa_J61117
type: ComponentDefinition
persistent identity: BBa J61117
display id: BBa_J61117
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ribosome Binding Site Family Member
creator: John Anderson
created: 2007-04-23T11:00:00Z
modified: 2015-08-31T01:59:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable description: {{JCA_Arkin_RBSFamily}}
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J61117_sequence/1

BBa_K1468001
type: ComponentDefinition
persistent identity: BBa K1468001
display id: BBa_K1468001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pJ23104 + gene encoding AsRed2
creator: Pedro Luis Dorado Morales
created: 2014-10-02T11:00:00Z
modified: 2015-05-08T01:10:38Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1847_
m user id: 0
owner id: 11756
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: -
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence: https://synbiohub.org/public/igem/BBa_K1468001_sequence/1

BBa_J61122
type: ComponentDefinition
persistent identity: BBa J61122
display id: BBa_J61122
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ribosome Binding Site Family Member
creator: John Anderson
created: 2007-04-24T11:00:00Z
modified: 2015-08-31T01:59:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: {{JCA_Arkin_RBSFamily}}
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J61122_sequence/1

BBa_J61102
type: ComponentDefinition
persistent identity: BBa J61102
display id: BBa_J61102
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ribosome Binding Site Family Member
creator: John Anderson
created: 2007-01-28T12:00:00Z
modified: 2015-08-31T02:03:00Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable description: {{JCA_Arkin_RBSFamily}}
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J61102_sequence/1

BBa_K1709001
type: ComponentDefinition
persistent identity: BBa K1709001
display id: BBa_K1709001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LuxI-His
creator: Astrid Deryckere
created: 2015-09-07T11:00:00Z
modified: 2015-09-26T09:17:10Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2129_
m user id: 25069
owner id: 25389
owning group id: 9
status: status/Planning
bookmark: true
mutable description: Autoinducer synthetase for acyl-homoserine lactones (AHL): LuxI synthesizes AHL (3OC6HSL). Two molecules of AHL form a complex with two molecules of the LuxR protein. This complex then binds to a palyndromic site on the lux promoter, increasing its transcription rate. LuxI, LuxR and the right lux promoter are all derived from V. fischeri. In absence of AHL, the right lux promotor only gives weak constitutive expression of downstream genes. The LuxI gene is fused to a His-tag facilitating gene expression measurements.
mutable notes: Biobrick BBa_C0061 still contained a Barcode sequence which was deleted. Also, a Ribosome Binding Site was added. For our own use, 5 bp were exchanged to create unique restriction sites and to optimize DNA folding.
mutable provenance: Sequence is originating from V. fischeri. Here, the sequence from BBa_C0061 was used and optimized.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K1709000
component: BBa_J61104
sequence annotation: BBa_J61104
sequence annotation: BBa_K1709000
sequence: https://synbiohub.org/public/igem/BBa_K1709001_sequence/1

BBa_K1709000
type: Component
persistent identity: component2473568
display id: component2473568
version: 1
top level: BBa_K1709001
access: public
definition: https://synbiohub.org/public/igem/BBa_K1709000/1

BBa_J61104
type: Component
persistent identity: component2473563
display id: component2473563
version: 1
top level: BBa_K1709001
access: public
definition: BBa_J61104

BBa_J61104
type: SequenceAnnotation
persistent identity: annotation2473563
display id: annotation2473563
version: 1
top level: BBa_K1709001
location: https://synbiohub.org/public/igem/BBa_K1709001/annotation2473563/range2473563/1
component: BBa_J61104

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display id: range2473563
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top level: BBa_K1709001
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BBa_K1709000
type: SequenceAnnotation
persistent identity: annotation2473568
display id: annotation2473568
version: 1
top level: BBa_K1709001
location: https://synbiohub.org/public/igem/BBa_K1709001/annotation2473568/range2473568/1
component: BBa_K1709000

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type: Range
persistent identity: range2473568
display id: range2473568
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direction: direction/reverse
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start: 19
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BBa_K356101
type: ComponentDefinition
persistent identity: BBa K356101
display id: BBa_K356101
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PAnderRBSGFP
creator: Fatemeh Salimi
created: 2010-10-23T11:00:00Z
modified: 2015-05-08T01:12:11Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _467_
m user id: 0
owner id: 7756
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: Promoter Anderson RBS GFP double terminator
mutable notes: promoter and RBS designed
mutable provenance: This is an RBS from the Anderson catalogue.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_B0012
component: BBa_B0010
component: BBa_K356106
component: BBa_J23100
component: BBa_E0040
sequence annotation: BBa_B0012
sequence annotation: BBa_E0040
sequence annotation: BBa_J23100
sequence annotation: BBa_K356106
sequence annotation: BBa_B0010
sequence: https://synbiohub.org/public/igem/BBa_K356101_sequence/1

BBa_B0012
type: Component
persistent identity: component2107759
display id: component2107759
version: 1
top level: BBa_K356101
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0010
type: Component
persistent identity: component2107757
display id: component2107757
version: 1
top level: BBa_K356101
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_K356106
type: Component
persistent identity: component2107754
display id: component2107754
version: 1
top level: BBa_K356101
access: public
definition: BBa_K356106

BBa_J23100
type: Component
persistent identity: component2107753
display id: component2107753
version: 1
top level: BBa_K356101
access: public
definition: https://synbiohub.org/public/igem/BBa_J23100/1

BBa_E0040
type: Component
persistent identity: component2107756
display id: component2107756
version: 1
top level: BBa_K356101
access: public
definition: https://synbiohub.org/public/igem/BBa_E0040/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2107759
display id: annotation2107759
version: 1
top level: BBa_K356101
location: https://synbiohub.org/public/igem/BBa_K356101/annotation2107759/range2107759/1
component: BBa_B0012

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type: Range
persistent identity: range2107759
display id: range2107759
version: 1
direction: direction/forward
top level: BBa_K356101
start: 882
end: 922
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation2107756
display id: annotation2107756
version: 1
top level: BBa_K356101
location: https://synbiohub.org/public/igem/BBa_K356101/annotation2107756/range2107756/1
component: BBa_E0040

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type: Range
persistent identity: range2107756
display id: range2107756
version: 1
direction: direction/reverse
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start: 66
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orientation: inline

BBa_J23100
type: SequenceAnnotation
persistent identity: annotation2107753
display id: annotation2107753
version: 1
top level: BBa_K356101
location: https://synbiohub.org/public/igem/BBa_K356101/annotation2107753/range2107753/1
component: BBa_J23100

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type: Range
persistent identity: range2107753
display id: range2107753
version: 1
direction: direction/reverse
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start: 1
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BBa_K356106
type: SequenceAnnotation
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display id: annotation2107754
version: 1
top level: BBa_K356101
location: https://synbiohub.org/public/igem/BBa_K356101/annotation2107754/range2107754/1
component: BBa_K356106

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type: Range
persistent identity: range2107754
display id: range2107754
version: 1
direction: direction/reverse
top level: BBa_K356101
start: 44
end: 59
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2107757
display id: annotation2107757
version: 1
top level: BBa_K356101
location: https://synbiohub.org/public/igem/BBa_K356101/annotation2107757/range2107757/1
component: BBa_B0010

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type: Range
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display id: range2107757
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direction: direction/forward
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BBa_J61112
type: ComponentDefinition
persistent identity: BBa J61112
display id: BBa_J61112
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ribosome Binding Site Family Member
creator: John Anderson
created: 2007-04-23T11:00:00Z
modified: 2015-08-31T02:03:00Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable description: {{JCA_Arkin_RBSFamily}}
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J61112_sequence/1

BBa_J61139
type: ComponentDefinition
persistent identity: BBa J61139
display id: BBa_J61139
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ribosome Binding Site Family Member
creator: John Anderson
created: 2007-04-24T11:00:00Z
modified: 2015-08-31T01:59:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable description: {{JCA_Arkin_RBSFamily}}
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J61139_sequence/1

BBa_J61125
type: ComponentDefinition
persistent identity: BBa J61125
display id: BBa_J61125
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ribosome Binding Site Family Member
creator: John Anderson
created: 2007-04-24T11:00:00Z
modified: 2015-08-31T01:59:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable description: {{JCA_Arkin_RBSFamily}}
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J61125_sequence/1

BBa_J61105
type: ComponentDefinition
persistent identity: BBa J61105
display id: BBa_J61105
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ribosome Binding Site Family Member
creator: John Anderson
created: 2007-04-23T11:00:00Z
modified: 2015-08-31T02:03:00Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable description: {{JCA_Arkin_RBSFamily}}
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J61105_sequence/1

BBa_J61130
type: ComponentDefinition
persistent identity: BBa J61130
display id: BBa_J61130
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ribosome Binding Site Family Member
creator: John Anderson
created: 2007-04-24T11:00:00Z
modified: 2015-08-31T01:59:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable description: {{JCA_Arkin_RBSFamily}}
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J61130_sequence/1

BBa_K356106
type: ComponentDefinition
persistent identity: BBa K356106
display id: BBa_K356106
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Desinged RBS
creator: Fatemeh Salimi
created: 2010-10-25T11:00:00Z
modified: 2015-05-08T01:12:11Z
discontinued: false
dominant: false
experience: experience/None
group u list: _467_
m user id: 0
owner id: 6685
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: Designed RBS
mutable notes: primers
mutable provenance: RBS calculator
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_K356106_sequence/1

BBa_J61136
type: ComponentDefinition
persistent identity: BBa J61136
display id: BBa_J61136
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ribosome Binding Site Family Member
creator: John Anderson
created: 2007-04-24T11:00:00Z
modified: 2015-08-31T01:59:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable description: {{JCA_Arkin_RBSFamily}}
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J61136_sequence/1

BBa_J61133
type: ComponentDefinition
persistent identity: BBa J61133
display id: BBa_J61133
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ribosome Binding Site Family Member
creator: John Anderson
created: 2007-04-24T11:00:00Z
modified: 2015-08-31T01:59:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable description: {{JCA_Arkin_RBSFamily}}
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J61133_sequence/1

BBa_J61108
type: ComponentDefinition
persistent identity: BBa J61108
display id: BBa_J61108
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ribosome Binding Site Family Member
creator: John Anderson
created: 2007-04-23T11:00:00Z
modified: 2015-08-31T02:03:00Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable description: {{JCA_Arkin_RBSFamily}}
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J61108_sequence/1