//proteindomain/tail
type: Collection
persistent identity: proteindomain tail
display id: proteindomain_tail
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
owned by: james
owned by: myers
member: BBa_K112603
member: 3x FLAG
member: BBa_K1085009
member: Strep-tag
member: PolyHb
member: HA tag
member: V5 tag
member: FLAG
member: BBa_K772008
member: PolyWb
member: His-Stop
member: VSV-G tag
member: LigA
member: 7x His
member: BBa_K772009
member: HisTag
member: BBa_K112601
member: RGD-His-St
member: TrpZipper2
member: BBa_K1085008
member: BBa_K112503
member: BBa_K112507
member: S-tag
member: c-Myc
member: BBa_K1319007

BBa_K112507
type: ComponentDefinition
persistent identity: BBa K112507
display id: BBa_K112507
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: < HA ! in BBb
creator: Sherine Cheung
created: 2008-10-21T11:00:00Z
modified: 2015-05-08T01:09:18Z
discontinued: false
dominant: false
experience: experience/None
group u list: _224_
m user id: 0
owner id: 3386
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This is an HA tag without a start, but with a stop codon. It is in BBb format. ---- This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at: [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
mutable notes: N/A
mutable provenance: Construction of {<HA!} basic part K112507 Wobble PCR of cc001/cc011 ( 54 bp, EcoRI/BamHI) Sub into pBca1256 (EcoRI/BamHI, 2227+ 910,L) Product is pBca1256-K112507 -------------------------------------------- cc001 Forward Biobricking of {<HA>} CgATAgaattcATGagatcttacccatacgacgtcccagac cc011 Reverse biobricking of {<HA!} ccagtggatccttacccagcgtagtctgggacgtcgtatgggtaag
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000324
role: partType/Tag
sequence: https://synbiohub.org/public/igem/BBa_K112507_sequence/1

LigA
type: ComponentDefinition
persistent identity: BBa K525740
display id: BBa_K525740
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: DNA ligase from Escherichia coli (LigA) with His-tag
creator: Anna Drong
created: 2011-09-11T11:00:00Z
modified: 2015-05-08T01:12:35Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _690_
m user id: 0
owner id: 8543
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: LigA4 Freiburg Prefix, Suffix Histag
mutable notes: PCR
mutable provenance: Escherichia coli
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: NgoMIV
sequence annotation: His-tag
sequence annotation: ATG
sequence annotation: LigA
sequence: https://synbiohub.org/public/igem/BBa_K525740_sequence/1

NgoMIV
type: SequenceAnnotation
persistent identity: annotation2137456
display id: annotation2137456
version: 1
top level: LigA
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K525740/annotation2137456/range2137456/1

https://synbiohub.org/public/igem/BBa_K525740/annotation2137456/range2137456/1
type: Range
persistent identity: range2137456
display id: range2137456
version: 1
direction: direction/forward
top level: LigA
start: 4
end: 9
orientation: inline

His-tag
type: SequenceAnnotation
persistent identity: annotation2137505
display id: annotation2137505
version: 1
top level: LigA
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K525740/annotation2137505/range2137505/1

https://synbiohub.org/public/igem/BBa_K525740/annotation2137505/range2137505/1
type: Range
persistent identity: range2137505
display id: range2137505
version: 1
direction: direction/forward
top level: LigA
start: 2020
end: 2037
orientation: inline

ATG
type: SequenceAnnotation
persistent identity: annotation2137503
display id: annotation2137503
version: 1
top level: LigA
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K525740/annotation2137503/range2137503/1

https://synbiohub.org/public/igem/BBa_K525740/annotation2137503/range2137503/1
type: Range
persistent identity: range2137503
display id: range2137503
version: 1
direction: direction/forward
top level: LigA
start: 1
end: 3
orientation: inline

LigA
type: SequenceAnnotation
persistent identity: annotation2137504
display id: annotation2137504
version: 1
top level: LigA
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K525740/annotation2137504/range2137504/1

https://synbiohub.org/public/igem/BBa_K525740/annotation2137504/range2137504/1
type: Range
persistent identity: range2137504
display id: range2137504
version: 1
direction: direction/forward
top level: LigA
start: 1
end: 2018
orientation: inline

BBa_K1319007
type: ComponentDefinition
persistent identity: BBa K1319007
display id: BBa_K1319007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: 6xHis tag with double stop codon
creator: Michael Osthege
created: 2014-09-24T11:00:00Z
modified: 2015-05-08T01:09:50Z
discontinued: false
dominant: false
group u list: _1694_
m user id: 0
owner id: 19522
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This part is a C-terminal 6xHis-tag with a double stop-codon. It can be fused to the C-terminus of proteins to enable purification of fully-translated proteins.
mutable notes: In contrast to other 6x-His tag parts, this part uses the standard double stop codon TAA-TAA.
mutable provenance: The part was designed using the histidin codon CAC and the double stop codons TAA-TAA.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000324
role: partType/Tag
sequence annotation: 6xHis
sequence: https://synbiohub.org/public/igem/BBa_K1319007_sequence/1

6xHis
type: SequenceAnnotation
persistent identity: annotation2386538
display id: annotation2386538
version: 1
top level: BBa_K1319007
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1319007/annotation2386538/range2386538/1

https://synbiohub.org/public/igem/BBa_K1319007/annotation2386538/range2386538/1
type: Range
persistent identity: range2386538
display id: range2386538
version: 1
direction: direction/forward
top level: BBa_K1319007
start: 1
end: 24
orientation: inline

BBa_K772009
type: ComponentDefinition
persistent identity: BBa K772009
display id: BBa_K772009
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: GFP - LasR protein complex with flag tags
creator: Mustafa Elitok
created: 2012-09-25T11:00:00Z
modified: 2015-05-08T01:13:15Z
discontinued: false
dominant: false
group u list: _1024_
m user id: 0
owner id: 9573
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This part contains a green fluorescent protein (GFP) as a non-reactive chemical compound (anchor) in order to neutralize the active reactive fixed to it, an inducer regulatory protein (LasR is responsible to induce pLas promoter in the presence of 6HSLO3 which is a chemical compound sythesized by an enzyme that LasI gene codes.) and a TEV protease cleavage site between them to assemble these two proteins. BBa_772001 and BBa_K772002 are assembled to be used as the trigger of detection in the whole system which ends with the intracellular transfection (or activation) of TEV protease. After the activation, it is planned that TEV protease will cleave this anchor-inducer protein complex to release free the reporter. In conclusion, in this system, the inducer would activate a transcription pathway on the targeted inducible gene or the reporter would indicate that there is a change in the whole system. This part contains flag tags extra from BBa_K772006 in the GFP region; because it is planned to be used in the Co-IP/SDS-PAGE assay to be isolated.
mutable notes: Note that there is an RBS sequence just before the protein sequence in order to eliminate the need of addition of it.
mutable provenance: Cleavage site, LasR and GFP sequences are gathered from Registry.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
sequence annotation: LasR
sequence annotation: RBS
sequence annotation: Anchor-inducer + flag
sequence annotation: TEV cleavage site
sequence annotation: Double terminator
sequence annotation: Flag tag
sequence annotation: GFP
sequence: https://synbiohub.org/public/igem/BBa_K772009_sequence/1

LasR
type: SequenceAnnotation
persistent identity: annotation2203921
display id: annotation2203921
version: 1
top level: BBa_K772009
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K772009/annotation2203921/range2203921/1

https://synbiohub.org/public/igem/BBa_K772009/annotation2203921/range2203921/1
type: Range
persistent identity: range2203921
display id: range2203921
version: 1
direction: direction/forward
top level: BBa_K772009
start: 858
end: 1614
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2203917
display id: annotation2203917
version: 1
top level: BBa_K772009
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K772009/annotation2203917/range2203917/1

https://synbiohub.org/public/igem/BBa_K772009/annotation2203917/range2203917/1
type: Range
persistent identity: range2203917
display id: range2203917
version: 1
direction: direction/forward
top level: BBa_K772009
start: 1
end: 20
orientation: inline

Anchor-inducer + flag
type: SequenceAnnotation
persistent identity: annotation2203923
display id: annotation2203923
version: 1
top level: BBa_K772009
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K772009/annotation2203923/range2203923/1

https://synbiohub.org/public/igem/BBa_K772009/annotation2203923/range2203923/1
type: Range
persistent identity: range2203923
display id: range2203923
version: 1
direction: direction/forward
top level: BBa_K772009
start: 21
end: 1614
orientation: inline

TEV cleavage site
type: SequenceAnnotation
persistent identity: annotation2203920
display id: annotation2203920
version: 1
top level: BBa_K772009
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K772009/annotation2203920/range2203920/1

https://synbiohub.org/public/igem/BBa_K772009/annotation2203920/range2203920/1
type: Range
persistent identity: range2203920
display id: range2203920
version: 1
direction: direction/forward
top level: BBa_K772009
start: 768
end: 857
orientation: inline

Double terminator
type: SequenceAnnotation
persistent identity: annotation2203922
display id: annotation2203922
version: 1
top level: BBa_K772009
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K772009/annotation2203922/range2203922/1

https://synbiohub.org/public/igem/BBa_K772009/annotation2203922/range2203922/1
type: Range
persistent identity: range2203922
display id: range2203922
version: 1
direction: direction/forward
top level: BBa_K772009
start: 1615
end: 1745
orientation: inline

Flag tag
type: SequenceAnnotation
persistent identity: annotation2203918
display id: annotation2203918
version: 1
top level: BBa_K772009
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K772009/annotation2203918/range2203918/1

https://synbiohub.org/public/igem/BBa_K772009/annotation2203918/range2203918/1
type: Range
persistent identity: range2203918
display id: range2203918
version: 1
direction: direction/forward
top level: BBa_K772009
start: 21
end: 46
orientation: inline

GFP
type: SequenceAnnotation
persistent identity: annotation2203919
display id: annotation2203919
version: 1
top level: BBa_K772009
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K772009/annotation2203919/range2203919/1

https://synbiohub.org/public/igem/BBa_K772009/annotation2203919/range2203919/1
type: Range
persistent identity: range2203919
display id: range2203919
version: 1
direction: direction/forward
top level: BBa_K772009
start: 47
end: 767
orientation: inline

RGD-His-St
type: ComponentDefinition
persistent identity: BBa K133036
display id: BBa_K133036
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RGD-His affinity tag-Stop
creator: Jerneja Mori
created: 2008-10-25T11:00:00Z
modified: 2015-05-08T01:09:55Z
discontinued: false
dominant: false
experience: experience/None
group u list: _231_
m user id: 0
owner id: 3350
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This is His-tag with stop codon and RGD seguence. His-tag is used for detection of His-tagged proteins by anti-His antibodies. RGD is arginine-glycine-aspartic amino acid sequence, present on several extracellular matrix proteins. It is required for binding to integrins.
mutable notes: Cloned into pSB1AK3 vector.
mutable provenance: Part was constructed by primer annealing.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000324
role: partType/Tag
sequence: https://synbiohub.org/public/igem/BBa_K133036_sequence/1

V5 tag
type: ComponentDefinition
persistent identity: BBa T2018
display id: BBa_T2018
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: V5 epitope tag tail domain (GKPIPNPLLGLDST)
creator: Reshma Shetty
created: 2009-09-02T11:00:00Z
modified: 2015-05-08T01:14:52Z
discontinued: false
dominant: false
experience: experience/None
group u list: _41_
m user id: 0
owner id: 126
owning group id: 162
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is a V5 epitope tag tail domain designed to be at the C-terminus of a protein. V5 tags are a type of epitope tag that can be used to measure the quantity of proteins.
mutable notes: A double TAATAA stop codon was included. Rare codons from common model organisms were avoided.
mutable provenance: This is a synthetic sequence.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: V5 epitope tag
sequence annotation: double stop codon
sequence: https://synbiohub.org/public/igem/BBa_T2018_sequence/1

V5 epitope tag
type: SequenceAnnotation
persistent identity: annotation2018275
display id: annotation2018275
version: 1
top level: V5 tag
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_T2018/annotation2018275/range2018275/1

https://synbiohub.org/public/igem/BBa_T2018/annotation2018275/range2018275/1
type: Range
persistent identity: range2018275
display id: range2018275
version: 1
direction: direction/forward
top level: V5 tag
start: 1
end: 42
orientation: inline

double stop codon
type: SequenceAnnotation
persistent identity: annotation2018276
display id: annotation2018276
version: 1
top level: V5 tag
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_T2018/annotation2018276/range2018276/1

https://synbiohub.org/public/igem/BBa_T2018/annotation2018276/range2018276/1
type: Range
persistent identity: range2018276
display id: range2018276
version: 1
direction: direction/forward
top level: V5 tag
start: 43
end: 48
orientation: inline

HisTag
type: ComponentDefinition
persistent identity: BBa K792005
display id: BBa_K792005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Histidine rich peptide (His Tag)
creator: Manuel Gim??nez
created: 2012-09-24T11:00:00Z
modified: 2015-05-08T01:13:23Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1047_
m user id: 0
owner id: 11811
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Stable and soluble Histidine rich peptide.
mutable notes: Retro-translated and optimized (codons and mRNA seconday structure) for yeast.
mutable provenance: Clontech???s His-tag.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: HindIII restriction site
sequence annotation: stop
sequence annotation: His tag
sequence: https://synbiohub.org/public/igem/BBa_K792005_sequence/1

HindIII restriction site
type: SequenceAnnotation
persistent identity: annotation2196426
display id: annotation2196426
version: 1
top level: HisTag
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K792005/annotation2196426/range2196426/1

https://synbiohub.org/public/igem/BBa_K792005/annotation2196426/range2196426/1
type: Range
persistent identity: range2196426
display id: range2196426
version: 1
direction: direction/forward
top level: HisTag
start: 1
end: 6
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2196557
display id: annotation2196557
version: 1
top level: HisTag
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K792005/annotation2196557/range2196557/1

https://synbiohub.org/public/igem/BBa_K792005/annotation2196557/range2196557/1
type: Range
persistent identity: range2196557
display id: range2196557
version: 1
direction: direction/forward
top level: HisTag
start: 43
end: 48
orientation: inline

His tag
type: SequenceAnnotation
persistent identity: annotation2197882
display id: annotation2197882
version: 1
top level: HisTag
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K792005/annotation2197882/range2197882/1

https://synbiohub.org/public/igem/BBa_K792005/annotation2197882/range2197882/1
type: Range
persistent identity: range2197882
display id: range2197882
version: 1
direction: direction/forward
top level: HisTag
start: 7
end: 42
orientation: inline

LigA
type: ComponentDefinition
persistent identity: BBa K525710
display id: BBa_K525710
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: DNA ligase from Escherichia coli (LigA) with PT7, RBS and His-tag
creator: Anna Drong
created: 2011-09-11T11:00:00Z
modified: 2015-05-08T01:12:35Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _690_
m user id: 0
owner id: 8543
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: LigA1 mit Promotor, RBS, Histag
mutable notes: PCR
mutable provenance: Escherichia coli
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: His-tag
sequence annotation: RBS
sequence annotation: T7 promoter
sequence annotation: ATG
sequence annotation: B0034
sequence annotation: LigA
sequence: https://synbiohub.org/public/igem/BBa_K525710_sequence/1

His-tag
type: SequenceAnnotation
persistent identity: annotation2161304
display id: annotation2161304
version: 1
top level: LigA
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K525710/annotation2161304/range2161304/1

https://synbiohub.org/public/igem/BBa_K525710/annotation2161304/range2161304/1
type: Range
persistent identity: range2161304
display id: range2161304
version: 1
direction: direction/forward
top level: LigA
start: 2047
end: 2064
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2161301
display id: annotation2161301
version: 1
top level: LigA
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K525710/annotation2161301/range2161301/1

https://synbiohub.org/public/igem/BBa_K525710/annotation2161301/range2161301/1
type: Range
persistent identity: range2161301
display id: range2161301
version: 1
direction: direction/forward
top level: LigA
start: 24
end: 27
orientation: inline

T7 promoter
type: SequenceAnnotation
persistent identity: annotation2161299
display id: annotation2161299
version: 1
top level: LigA
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K525710/annotation2161299/range2161299/1

https://synbiohub.org/public/igem/BBa_K525710/annotation2161299/range2161299/1
type: Range
persistent identity: range2161299
display id: range2161299
version: 1
direction: direction/forward
top level: LigA
start: 1
end: 20
orientation: inline

ATG
type: SequenceAnnotation
persistent identity: annotation2161302
display id: annotation2161302
version: 1
top level: LigA
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K525710/annotation2161302/range2161302/1

https://synbiohub.org/public/igem/BBa_K525710/annotation2161302/range2161302/1
type: Range
persistent identity: range2161302
display id: range2161302
version: 1
direction: direction/forward
top level: LigA
start: 34
end: 36
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation2161300
display id: annotation2161300
version: 1
top level: LigA
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K525710/annotation2161300/range2161300/1

https://synbiohub.org/public/igem/BBa_K525710/annotation2161300/range2161300/1
type: Range
persistent identity: range2161300
display id: range2161300
version: 1
direction: direction/forward
top level: LigA
start: 21
end: 33
orientation: inline

LigA
type: SequenceAnnotation
persistent identity: annotation2161303
display id: annotation2161303
version: 1
top level: LigA
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K525710/annotation2161303/range2161303/1

https://synbiohub.org/public/igem/BBa_K525710/annotation2161303/range2161303/1
type: Range
persistent identity: range2161303
display id: range2161303
version: 1
direction: direction/forward
top level: LigA
start: 34
end: 2045
orientation: inline

BBa_K112601
type: ComponentDefinition
persistent identity: BBa K112601
display id: BBa_K112601
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: < AP tag ! in BBb
creator: Sherine Cheung
created: 2008-09-02T11:00:00Z
modified: 2015-05-08T01:09:18Z
discontinued: false
dominant: false
experience: experience/None
group u list: _224_
m user id: 0
owner id: 3386
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This is an AP tag with stop codon, but no start. ---- This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at: [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
mutable notes: N/A
mutable provenance: Construction of {<AP!} basic part K112601 Wobble PCR SC001/SC003 (69 bp, EcoRI/BamHI) Sub into pBca1256 (EcoRI/BamHI, 2472+ 910) Product is pBca1256-K112601 -------------------------------------------------- SC001 Forward Biobricking of <AP-tag! cgataGAATTCatgAGATCTggcctgaacgatatttttgaagcgcag SC003 Reverse Biobricking of <AP-tag! ccagtGGATCCttattcatgccattcaattttctgcgcttcaaaaatatcg
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000324
role: partType/Tag
sequence: https://synbiohub.org/public/igem/BBa_K112601_sequence/1

BBa_K1085009
type: ComponentDefinition
persistent identity: BBa K1085009
display id: BBa_K1085009
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: SilkTail Strep-tag Stop-codon
creator: Claudio Tiecher
created: 2013-09-09T11:00:00Z
modified: 2015-06-02T12:11:00Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1395_
m user id: 4206
owner id: 16089
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This BioBrick contains the coding sequence for silk tail with a Strep-tag behind it and ends with a stop codon. The Tail ??????. The Strep-tag, fused at the C terminal, is used both as a binding tag to use to inidicate its production and as a binding component to biotin.
mutable notes: The DNA sequence was codon-optimized for Bacillus Subtilis. Moreover the nucleotide sequence was optimized in order to accomplish the production standard of IDT. A couple of optimized codons were affected by this.
mutable provenance: The amino acid sequence of the tail was obtained from MaSp2. The DNA sequence was synthesized by Integrated DNA Technologies (IDT).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: Stop codon (TGA)
sequence annotation: strep
sequence annotation: spidersilk C-terminal domain
sequence: https://synbiohub.org/public/igem/BBa_K1085009_sequence/1

Stop codon (TGA)
type: SequenceAnnotation
persistent identity: annotation2362391
display id: annotation2362391
version: 1
top level: BBa_K1085009
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1085009/annotation2362391/range2362391/1

https://synbiohub.org/public/igem/BBa_K1085009/annotation2362391/range2362391/1
type: Range
persistent identity: range2362391
display id: range2362391
version: 1
direction: direction/forward
top level: BBa_K1085009
start: 313
end: 315
orientation: inline

strep
type: SequenceAnnotation
persistent identity: annotation2339061
display id: annotation2339061
version: 1
top level: BBa_K1085009
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1085009/annotation2339061/range2339061/1

https://synbiohub.org/public/igem/BBa_K1085009/annotation2339061/range2339061/1
type: Range
persistent identity: range2339061
display id: range2339061
version: 1
direction: direction/forward
top level: BBa_K1085009
start: 289
end: 312
orientation: inline

spidersilk C-terminal domain
type: SequenceAnnotation
persistent identity: annotation2339062
display id: annotation2339062
version: 1
top level: BBa_K1085009
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1085009/annotation2339062/range2339062/1

https://synbiohub.org/public/igem/BBa_K1085009/annotation2339062/range2339062/1
type: Range
persistent identity: range2339062
display id: range2339062
version: 1
direction: direction/forward
top level: BBa_K1085009
start: 1
end: 270
orientation: inline

3x FLAG
type: ComponentDefinition
persistent identity: BBa J64005
display id: BBa_J64005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: 3x FLAG affinity tag
creator: Dan Widmaier
created: 2008-01-15T12:00:00Z
modified: 2015-08-31T01:56:26Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _98_
m user id: 0
owner id: 2407
owning group id: 98
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: A 3 repeat FLAG (DYKDDDDK) antigen epitope tag used for marking proteins of interest. Proteins carrying this tag can be easily detected using commercial western blotting reagents.
mutable notes: This sequence was designed as part of an oligonucleotide primer on each strand then annealed and ligated into the vector.
mutable provenance: Synthetic DNA from Oligonucleotide synthesis
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000324
role: partType/Tag
sequence annotation: STOP Codon
sequence annotation: 3x FLAG Tag
sequence annotation: XbaI Restriction Site
sequence: https://synbiohub.org/public/igem/BBa_J64005_sequence/1

STOP Codon
type: SequenceAnnotation
persistent identity: annotation1959596
display id: annotation1959596
version: 1
top level: 3x FLAG
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_J64005/annotation1959596/range1959596/1

https://synbiohub.org/public/igem/BBa_J64005/annotation1959596/range1959596/1
type: Range
persistent identity: range1959596
display id: range1959596
version: 1
direction: direction/forward
top level: 3x FLAG
start: 73
end: 75
orientation: inline

3x FLAG Tag
type: SequenceAnnotation
persistent identity: annotation1959595
display id: annotation1959595
version: 1
top level: 3x FLAG
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_J64005/annotation1959595/range1959595/1

https://synbiohub.org/public/igem/BBa_J64005/annotation1959595/range1959595/1
type: Range
persistent identity: range1959595
display id: range1959595
version: 1
direction: direction/forward
top level: 3x FLAG
start: 1
end: 72
orientation: inline

XbaI Restriction Site
type: SequenceAnnotation
persistent identity: annotation1960214
display id: annotation1960214
version: 1
top level: 3x FLAG
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J64005/annotation1960214/range1960214/1

https://synbiohub.org/public/igem/BBa_J64005/annotation1960214/range1960214/1
type: Range
persistent identity: range1960214
display id: range1960214
version: 1
direction: direction/forward
top level: 3x FLAG
start: 76
end: 81
orientation: inline

BBa_K112603
type: ComponentDefinition
persistent identity: BBa K112603
display id: BBa_K112603
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: < FLAG tag ! in BBb
creator: Sherine Cheung
created: 2008-09-02T11:00:00Z
modified: 2015-05-08T01:09:18Z
discontinued: false
dominant: false
experience: experience/None
group u list: _224_
m user id: 0
owner id: 3386
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This is a FLAG tag with a stop codon, but no start codon. ---- This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at: [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
mutable notes: N/A
mutable provenance: Construction of {<FLAG!} basic part K112603 Wobble PCR SC004/SC006 (48 bp, EcoRI/BamHI) Sub into pBca1256 (EcoRI/BamHI, 2472+ 910) Product is pBca1256-K112603 ----------------------------------------------------- SC004 Forward Biobricking of <FLAG-tag! cgataGAATTCatgAGATCTgactacaaggatgacgacg SC006 Reverse Biobricking of <FLAG-tag! ccagtGGATCCttacttgtcgtcgtcatccttgtagtc
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000324
role: partType/Tag
sequence: https://synbiohub.org/public/igem/BBa_K112603_sequence/1

S-tag
type: ComponentDefinition
persistent identity: BBa T2015
display id: BBa_T2015
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: S-tag tail domain (KETAAAKFERQHMDS)
creator: Reshma Shetty
created: 2009-09-01T11:00:00Z
modified: 2015-05-08T01:14:52Z
discontinued: false
dominant: false
experience: experience/None
group u list: _41_
m user id: 0
owner id: 126
owning group id: 162
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is a S-tag tail domain designed to be at the C-terminus of a protein. S-tags are a type of affinity tag that can be used to purify and/or measure the quantity of proteins.
mutable notes: Included a double TAATAA stop codon.
mutable provenance: This is a synthetic sequence.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: S-tag
sequence annotation: double stop codon
sequence: https://synbiohub.org/public/igem/BBa_T2015_sequence/1

S-tag
type: SequenceAnnotation
persistent identity: annotation2018265
display id: annotation2018265
version: 1
top level: S-tag
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_T2015/annotation2018265/range2018265/1

https://synbiohub.org/public/igem/BBa_T2015/annotation2018265/range2018265/1
type: Range
persistent identity: range2018265
display id: range2018265
version: 1
direction: direction/forward
top level: S-tag
start: 1
end: 45
orientation: inline

double stop codon
type: SequenceAnnotation
persistent identity: annotation2018266
display id: annotation2018266
version: 1
top level: S-tag
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_T2015/annotation2018266/range2018266/1

https://synbiohub.org/public/igem/BBa_T2015/annotation2018266/range2018266/1
type: Range
persistent identity: range2018266
display id: range2018266
version: 1
direction: direction/forward
top level: S-tag
start: 46
end: 51
orientation: inline

VSV-G tag
type: ComponentDefinition
persistent identity: BBa T2021
display id: BBa_T2021
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: VSV-G epitope tag tail domain (YTDIEMNRLGK)
creator: Reshma Shetty
created: 2009-09-02T11:00:00Z
modified: 2015-05-08T01:14:52Z
discontinued: false
dominant: false
experience: experience/None
group u list: _41_
m user id: 0
owner id: 126
owning group id: 162
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is a VSV-G epitope tag tail domain designed to be at the C-terminus of a protein. VSV-G tags are a type of epitope tag that can be used to measure the quantity of proteins.
mutable notes: A double TAA TAA stop codon was used. Rare codons from model organisms were avoided where possible.
mutable provenance: This is a synthetic sequence
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: double TAA stop codon
sequence annotation: VSV-G epitope tag
sequence: https://synbiohub.org/public/igem/BBa_T2021_sequence/1

double TAA stop codon
type: SequenceAnnotation
persistent identity: annotation2018282
display id: annotation2018282
version: 1
top level: VSV-G tag
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_T2021/annotation2018282/range2018282/1

https://synbiohub.org/public/igem/BBa_T2021/annotation2018282/range2018282/1
type: Range
persistent identity: range2018282
display id: range2018282
version: 1
direction: direction/forward
top level: VSV-G tag
start: 34
end: 39
orientation: inline

VSV-G epitope tag
type: SequenceAnnotation
persistent identity: annotation2018281
display id: annotation2018281
version: 1
top level: VSV-G tag
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_T2021/annotation2018281/range2018281/1

https://synbiohub.org/public/igem/BBa_T2021/annotation2018281/range2018281/1
type: Range
persistent identity: range2018281
display id: range2018281
version: 1
direction: direction/forward
top level: VSV-G tag
start: 1
end: 33
orientation: inline

His-Stop
type: ComponentDefinition
persistent identity: BBa K133133
display id: BBa_K133133
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: His affinity tag-stop
creator: Katja Kolar
created: 2008-10-22T11:00:00Z
modified: 2015-05-08T01:09:57Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _231_
m user id: 0
owner id: 1883
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This is His-tag with stop codon. His-tag is used for detection of His-tagged proteins by anti-His antibodies. Cloned into pSB1AK3 vector.
mutable provenance: Part was constructed by primer annealing.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000324
role: partType/Tag
sequence: https://synbiohub.org/public/igem/BBa_K133133_sequence/1

7x His
type: ComponentDefinition
persistent identity: BBa T2000
display id: BBa_T2000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: 7x His tag tail domain
creator: Reshma Shetty
created: 2009-08-10T11:00:00Z
modified: 2015-05-08T01:14:52Z
discontinued: false
dominant: true
experience: experience/None
group u list: _41_
m user id: 0
owner id: 126
owning group id: 162
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is a 6x His tag tail domain designed to encode the C-terminus of a protein. His tags are a type of affinity tag that can be used to purify and/or measure the quantity of proteins.
mutable notes: Used both His codons in order to avoid repeats. Also included a double TAATAA stop codon.
mutable provenance: No. This is a synthetic sequence.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: 7x His
sequence annotation: double stop codon
sequence: https://synbiohub.org/public/igem/BBa_T2000_sequence/1

7x His
type: SequenceAnnotation
persistent identity: annotation2016495
display id: annotation2016495
version: 1
top level: 7x His
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_T2000/annotation2016495/range2016495/1

https://synbiohub.org/public/igem/BBa_T2000/annotation2016495/range2016495/1
type: Range
persistent identity: range2016495
display id: range2016495
version: 1
direction: direction/forward
top level: 7x His
start: 1
end: 21
orientation: inline

double stop codon
type: SequenceAnnotation
persistent identity: annotation2016496
display id: annotation2016496
version: 1
top level: 7x His
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_T2000/annotation2016496/range2016496/1

https://synbiohub.org/public/igem/BBa_T2000/annotation2016496/range2016496/1
type: Range
persistent identity: range2016496
display id: range2016496
version: 1
direction: direction/forward
top level: 7x His
start: 22
end: 27
orientation: inline

BBa_K112503
type: ComponentDefinition
persistent identity: BBa K112503
display id: BBa_K112503
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: < myc ! in BBb
creator: Sherine Cheung
created: 2008-10-21T11:00:00Z
modified: 2015-05-08T01:09:18Z
discontinued: false
dominant: false
experience: experience/None
group u list: _224_
m user id: 0
owner id: 3386
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This is a myc tag without a start, but with a stop codon. It is in BBb format. ---- This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at: [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
mutable notes: N/A
mutable provenance: Construction of {<myc!} basic part K112503 Wobble PCR of cc004/cc006 ( 54 bp, EcoRI/BamHI) Sub into pBca1256 (EcoRI/BamHI,2227+ 910,L) Product is pBca1256-K112503 -------------------------------------------- cc004 Forward Biobricking of {<myc>} CgATAgaattcATGagatctGAACAAAAACTCATCTCAGAAGAGG cc006 Reverse Biobricking of {<myc!} ccagtggatccTTACAGATCCTCTTCTGAGATGAGTTTTTGTTC
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000324
role: partType/Tag
sequence: https://synbiohub.org/public/igem/BBa_K112503_sequence/1

TrpZipper2
type: ComponentDefinition
persistent identity: BBa K792006
display id: BBa_K792006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Tryptophan rich peptide (TrpZipper2)
creator: Manuel Gim??nez
created: 2012-09-24T11:00:00Z
modified: 2015-05-08T01:13:23Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1047_
m user id: 0
owner id: 11811
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: '''TrpZipper''' is a small peptide that folds into a beta-hairpin secondary structure. The indole rings of the Trp form a hydrophobic core. The protein is water soluble and monomer.
mutable notes: Sequence obtained by retro-translation. Codon and mRNA-secondary structure optimized for yeast.
mutable provenance: Tryptophan zippers: Stable, monomeric beta-hairpins [Cochran 2001]
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: stop
sequence annotation: cds
sequence annotation: HindIII restriction site
sequence: https://synbiohub.org/public/igem/BBa_K792006_sequence/1

stop
type: SequenceAnnotation
persistent identity: annotation2196616
display id: annotation2196616
version: 1
top level: TrpZipper2
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K792006/annotation2196616/range2196616/1

https://synbiohub.org/public/igem/BBa_K792006/annotation2196616/range2196616/1
type: Range
persistent identity: range2196616
display id: range2196616
version: 1
direction: direction/forward
top level: TrpZipper2
start: 43
end: 48
orientation: inline

cds
type: SequenceAnnotation
persistent identity: annotation2197860
display id: annotation2197860
version: 1
top level: TrpZipper2
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K792006/annotation2197860/range2197860/1

https://synbiohub.org/public/igem/BBa_K792006/annotation2197860/range2197860/1
type: Range
persistent identity: range2197860
display id: range2197860
version: 1
direction: direction/forward
top level: TrpZipper2
start: 7
end: 42
orientation: inline

HindIII restriction site
type: SequenceAnnotation
persistent identity: annotation2196458
display id: annotation2196458
version: 1
top level: TrpZipper2
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K792006/annotation2196458/range2196458/1

https://synbiohub.org/public/igem/BBa_K792006/annotation2196458/range2196458/1
type: Range
persistent identity: range2196458
display id: range2196458
version: 1
direction: direction/forward
top level: TrpZipper2
start: 1
end: 6
orientation: inline

HA tag
type: ComponentDefinition
persistent identity: BBa T2009
display id: BBa_T2009
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: hemagglutinin (HA) tag tail domain (YPYDVPDYA)
creator: Reshma Shetty
created: 2009-08-10T11:00:00Z
modified: 2015-05-08T01:14:52Z
discontinued: false
dominant: false
experience: experience/None
group u list: _41_
m user id: 0
owner id: 126
owning group id: 162
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is a HA tag tail domain designed to be at the C-terminus of a protein. HA tags are a type of affinity tag that can be used to purify and/or measure the quantity of proteins.
mutable notes: This domain encodes the HA sequence YPYDVPDYA. The codons at each amino acid position were selected to avoid rare codons as much as possible in organisms like ''E. coli'', humans, mouse, ''B. subtilis'', ''C. elegans'', ''Streptomyces coelicolor'' and other common model organisms and/or industrial hosts. Another factor in codon selection was avoiding avoid repeat sequences.
mutable provenance: This is a synthetic sequence.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: stop codon
sequence annotation: HA tag
sequence: https://synbiohub.org/public/igem/BBa_T2009_sequence/1

stop codon
type: SequenceAnnotation
persistent identity: annotation2017183
display id: annotation2017183
version: 1
top level: HA tag
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_T2009/annotation2017183/range2017183/1

https://synbiohub.org/public/igem/BBa_T2009/annotation2017183/range2017183/1
type: Range
persistent identity: range2017183
display id: range2017183
version: 1
direction: direction/forward
top level: HA tag
start: 28
end: 33
orientation: inline

HA tag
type: SequenceAnnotation
persistent identity: annotation2017182
display id: annotation2017182
version: 1
top level: HA tag
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_T2009/annotation2017182/range2017182/1

https://synbiohub.org/public/igem/BBa_T2009/annotation2017182/range2017182/1
type: Range
persistent identity: range2017182
display id: range2017182
version: 1
direction: direction/forward
top level: HA tag
start: 1
end: 27
orientation: inline

PolyHb
type: ComponentDefinition
persistent identity: BBa K792007
display id: BBa_K792007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Histidine rich peptide (PolyHb)
creator: Manuel Gim??nez
created: 2012-09-24T11:00:00Z
modified: 2015-05-08T01:13:23Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1047_
m user id: 0
owner id: 11811
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Stable and soluble Histidine rich peptide. This part has a '''HindIII''' restriction site as extra.
mutable notes: PolyHb was desing taking into acount the following consideration: # avoided repeating the same residue in tandem to minimize local tRNA depleation # avoided Trp in tandem because of therir low solubility # we included Gly to avoid the formation of stable structures # included acidic and basic amino acids to increase solubility Sequence obtained by retro-translation. Codon and mRNA-secondary structure optimized for yeast.
mutable provenance: Designed by BsAs Team 2012
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: stop
sequence annotation: cds
sequence annotation: HindIII restriction site
sequence: https://synbiohub.org/public/igem/BBa_K792007_sequence/1

stop
type: SequenceAnnotation
persistent identity: annotation2196617
display id: annotation2196617
version: 1
top level: PolyHb
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K792007/annotation2196617/range2196617/1

https://synbiohub.org/public/igem/BBa_K792007/annotation2196617/range2196617/1
type: Range
persistent identity: range2196617
display id: range2196617
version: 1
direction: direction/forward
top level: PolyHb
start: 115
end: 120
orientation: inline

cds
type: SequenceAnnotation
persistent identity: annotation2204263
display id: annotation2204263
version: 1
top level: PolyHb
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K792007/annotation2204263/range2204263/1

https://synbiohub.org/public/igem/BBa_K792007/annotation2204263/range2204263/1
type: Range
persistent identity: range2204263
display id: range2204263
version: 1
direction: direction/forward
top level: PolyHb
start: 7
end: 114
orientation: inline

HindIII restriction site
type: SequenceAnnotation
persistent identity: annotation2196465
display id: annotation2196465
version: 1
top level: PolyHb
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K792007/annotation2196465/range2196465/1

https://synbiohub.org/public/igem/BBa_K792007/annotation2196465/range2196465/1
type: Range
persistent identity: range2196465
display id: range2196465
version: 1
direction: direction/forward
top level: PolyHb
start: 1
end: 6
orientation: inline

FLAG
type: ComponentDefinition
persistent identity: BBa T2003
display id: BBa_T2003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: FLAG octapeptide tail domain (DYKDDDDK)
creator: Reshma Shetty
created: 2006-10-25T11:00:00Z
modified: 2015-05-08T01:14:52Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 25
owning group id: 1
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: kill this part
mutable notes: none
mutable provenance: none
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: FLAG tag octapeptide
sequence annotation: double stop codon
sequence: https://synbiohub.org/public/igem/BBa_T2003_sequence/1

FLAG tag octapeptide
type: SequenceAnnotation
persistent identity: annotation2016710
display id: annotation2016710
version: 1
top level: FLAG
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_T2003/annotation2016710/range2016710/1

https://synbiohub.org/public/igem/BBa_T2003/annotation2016710/range2016710/1
type: Range
persistent identity: range2016710
display id: range2016710
version: 1
direction: direction/forward
top level: FLAG
start: 1
end: 24
orientation: inline

double stop codon
type: SequenceAnnotation
persistent identity: annotation2016711
display id: annotation2016711
version: 1
top level: FLAG
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_T2003/annotation2016711/range2016711/1

https://synbiohub.org/public/igem/BBa_T2003/annotation2016711/range2016711/1
type: Range
persistent identity: range2016711
display id: range2016711
version: 1
direction: direction/forward
top level: FLAG
start: 25
end: 30
orientation: inline

c-Myc
type: ComponentDefinition
persistent identity: BBa T2006
display id: BBa_T2006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: c-Myc tail domain (EQKLISEEDL)
creator: Reshma Shetty
created: 2009-08-10T11:00:00Z
modified: 2015-05-08T01:14:52Z
discontinued: false
dominant: true
experience: experience/None
group u list: _41_
m user id: 0
owner id: 126
owning group id: 162
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is a c-Myc tag head domain designed to be at the C-terminus of a protein. c-Myc tags are a type of affinity tag that can be used to purify and/or measure the quantity of proteins.
mutable notes: This tail domain encodes the c-Myc sequence EQKLISEEDL. The codons at each amino acid position were selected to avoid rare codons in organisms like ''E. coli'', humans, mouse, ''B. subtilis'', ''C. elegans'', ''Streptomyces coelicolor'' and other common model organisms and/or industrial hosts.
mutable provenance: This is a synthetic sequence.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: double stop codon
sequence annotation: c-Myc tag
sequence: https://synbiohub.org/public/igem/BBa_T2006_sequence/1

double stop codon
type: SequenceAnnotation
persistent identity: annotation2017178
display id: annotation2017178
version: 1
top level: c-Myc
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_T2006/annotation2017178/range2017178/1

https://synbiohub.org/public/igem/BBa_T2006/annotation2017178/range2017178/1
type: Range
persistent identity: range2017178
display id: range2017178
version: 1
direction: direction/forward
top level: c-Myc
start: 31
end: 36
orientation: inline

c-Myc tag
type: SequenceAnnotation
persistent identity: annotation2017177
display id: annotation2017177
version: 1
top level: c-Myc
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_T2006/annotation2017177/range2017177/1

https://synbiohub.org/public/igem/BBa_T2006/annotation2017177/range2017177/1
type: Range
persistent identity: range2017177
display id: range2017177
version: 1
direction: direction/forward
top level: c-Myc
start: 1
end: 30
orientation: inline

BBa_K1085008
type: ComponentDefinition
persistent identity: BBa K1085008
display id: BBa_K1085008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: SilkTail Stop-codon
creator: Claudio Tiecher
created: 2013-09-09T11:00:00Z
modified: 2016-02-10T01:11:21Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1395_
m user id: 0
m user id: 4206
owner id: 16089
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This BioBrick contains the coding sequence for silk tail and ends with a stop codon. The Tail ....
mutable notes: The DNA sequence was codon-optimized for Bacillus Subtilis. Moreover the nucleotide sequence was optimized in order to accomplish the production standard of IDT. A couple of optimized codons were affected by this.
mutable provenance: The amino acid sequence of the tail was obtained from MaSp2. The DNA sequence was synthesized by Integrated DNA Technologies (IDT).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: Stop codon (TGA)
sequence annotation: spidersilk C-terminal domain
sequence: https://synbiohub.org/public/igem/BBa_K1085008_sequence/1

Stop codon (TGA)
type: SequenceAnnotation
persistent identity: annotation2362390
display id: annotation2362390
version: 1
top level: BBa_K1085008
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1085008/annotation2362390/range2362390/1

https://synbiohub.org/public/igem/BBa_K1085008/annotation2362390/range2362390/1
type: Range
persistent identity: range2362390
display id: range2362390
version: 1
direction: direction/forward
top level: BBa_K1085008
start: 271
end: 273
orientation: inline

spidersilk C-terminal domain
type: SequenceAnnotation
persistent identity: annotation2339058
display id: annotation2339058
version: 1
top level: BBa_K1085008
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1085008/annotation2339058/range2339058/1

https://synbiohub.org/public/igem/BBa_K1085008/annotation2339058/range2339058/1
type: Range
persistent identity: range2339058
display id: range2339058
version: 1
direction: direction/forward
top level: BBa_K1085008
start: 1
end: 270
orientation: inline

BBa_K772008
type: ComponentDefinition
persistent identity: BBa K772008
display id: BBa_K772008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: GFP - LacZ protein complex with flag tags
creator: Mustafa Elitok
created: 2012-09-25T11:00:00Z
modified: 2015-05-08T01:13:15Z
discontinued: false
dominant: false
group u list: _1024_
m user id: 0
owner id: 9573
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This part contains a green fluorescent protein (GFP) as a non-reactive chemical compound (anchor) in order to neutralize the active reactive fixed to it, a reporter device (LacZ enzyme catalyses the reaction of the cleavage of X-Gal which results with blue color change) and a TEV protease cleavage site between them to assemble these two proteins. BBa_772001 and BBa_K772002 are assembled to be used as the trigger of detection in the whole system which ends with the intracellular transfection (or activation) of TEV protease. After the activation, it is planned that TEV protease will cleave this anchor-inducer protein complex to release free the reporter. In conclusion, in this system, the inducer would activate a transcription pathway on the targeted inducible gene or the reporter would indicate that there is a change in the whole system. This part contains flag tags extra from BBa_K772006 in the GFP region; because it is planned to be used in the Co-IP/SDS-PAGE assay to be isolated.
mutable notes: Cleavage site, LacZ and GFP sequences are gathered from Registry.
mutable provenance: Note that there is an RBS sequence just before the protein sequence in order to eliminate the need of addition of it.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
sequence annotation: RBS
sequence annotation: Double Terminator
sequence annotation: Flag Tag
sequence annotation: LacZ
sequence annotation: GFP
sequence annotation: TEV cleavage site
sequence annotation: Anchor-Reporter + Flag
sequence: https://synbiohub.org/public/igem/BBa_K772008_sequence/1

RBS
type: SequenceAnnotation
persistent identity: annotation2203910
display id: annotation2203910
version: 1
top level: BBa_K772008
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K772008/annotation2203910/range2203910/1

https://synbiohub.org/public/igem/BBa_K772008/annotation2203910/range2203910/1
type: Range
persistent identity: range2203910
display id: range2203910
version: 1
direction: direction/forward
top level: BBa_K772008
start: 1
end: 20
orientation: inline

Double Terminator
type: SequenceAnnotation
persistent identity: annotation2203915
display id: annotation2203915
version: 1
top level: BBa_K772008
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K772008/annotation2203915/range2203915/1

https://synbiohub.org/public/igem/BBa_K772008/annotation2203915/range2203915/1
type: Range
persistent identity: range2203915
display id: range2203915
version: 1
direction: direction/forward
top level: BBa_K772008
start: 1102
end: 1232
orientation: inline

Flag Tag
type: SequenceAnnotation
persistent identity: annotation2203911
display id: annotation2203911
version: 1
top level: BBa_K772008
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K772008/annotation2203911/range2203911/1

https://synbiohub.org/public/igem/BBa_K772008/annotation2203911/range2203911/1
type: Range
persistent identity: range2203911
display id: range2203911
version: 1
direction: direction/forward
top level: BBa_K772008
start: 21
end: 46
orientation: inline

LacZ
type: SequenceAnnotation
persistent identity: annotation2203914
display id: annotation2203914
version: 1
top level: BBa_K772008
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K772008/annotation2203914/range2203914/1

https://synbiohub.org/public/igem/BBa_K772008/annotation2203914/range2203914/1
type: Range
persistent identity: range2203914
display id: range2203914
version: 1
direction: direction/forward
top level: BBa_K772008
start: 858
end: 1101
orientation: inline

GFP
type: SequenceAnnotation
persistent identity: annotation2203912
display id: annotation2203912
version: 1
top level: BBa_K772008
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K772008/annotation2203912/range2203912/1

https://synbiohub.org/public/igem/BBa_K772008/annotation2203912/range2203912/1
type: Range
persistent identity: range2203912
display id: range2203912
version: 1
direction: direction/forward
top level: BBa_K772008
start: 47
end: 767
orientation: inline

TEV cleavage site
type: SequenceAnnotation
persistent identity: annotation2203913
display id: annotation2203913
version: 1
top level: BBa_K772008
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K772008/annotation2203913/range2203913/1

https://synbiohub.org/public/igem/BBa_K772008/annotation2203913/range2203913/1
type: Range
persistent identity: range2203913
display id: range2203913
version: 1
direction: direction/forward
top level: BBa_K772008
start: 768
end: 857
orientation: inline

Anchor-Reporter + Flag
type: SequenceAnnotation
persistent identity: annotation2203916
display id: annotation2203916
version: 1
top level: BBa_K772008
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K772008/annotation2203916/range2203916/1

https://synbiohub.org/public/igem/BBa_K772008/annotation2203916/range2203916/1
type: Range
persistent identity: range2203916
display id: range2203916
version: 1
direction: direction/forward
top level: BBa_K772008
start: 21
end: 1101
orientation: inline

Strep-tag
type: ComponentDefinition
persistent identity: BBa T2012
display id: BBa_T2012
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Strep-tag tail domain (WRHPQFGG)
creator: Reshma Shetty
created: 2009-08-10T11:00:00Z
modified: 2015-05-08T01:14:52Z
discontinued: false
dominant: false
experience: experience/None
group u list: _41_
m user id: 0
owner id: 126
owning group id: 162
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is a Strep tag tail domain designed to be at the C-terminus of a protein. Strep tags are a type of affinity tag that can be used to purify and/or measure the quantity of proteins.
mutable notes: This domain encodes the Strep tag sequence AWRHPQFGG. The codons at each amino acid position were selected to avoid rare codons as much as possible in organisms like E. coli, humans, mouse, B. subtilis, C. elegans, Streptomyces coelicolor and other common model organisms and/or industrial hosts. Another factor in codon selection was avoiding avoid repeat sequences.
mutable provenance: This is a synthetic sequence.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: Strep-tag
sequence annotation: double stop codon
sequence: https://synbiohub.org/public/igem/BBa_T2012_sequence/1

Strep-tag
type: SequenceAnnotation
persistent identity: annotation2017187
display id: annotation2017187
version: 1
top level: Strep-tag
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_T2012/annotation2017187/range2017187/1

https://synbiohub.org/public/igem/BBa_T2012/annotation2017187/range2017187/1
type: Range
persistent identity: range2017187
display id: range2017187
version: 1
direction: direction/forward
top level: Strep-tag
start: 1
end: 24
orientation: inline

double stop codon
type: SequenceAnnotation
persistent identity: annotation2017188
display id: annotation2017188
version: 1
top level: Strep-tag
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_T2012/annotation2017188/range2017188/1

https://synbiohub.org/public/igem/BBa_T2012/annotation2017188/range2017188/1
type: Range
persistent identity: range2017188
display id: range2017188
version: 1
direction: direction/forward
top level: Strep-tag
start: 25
end: 30
orientation: inline

PolyWb
type: ComponentDefinition
persistent identity: BBa K792008
display id: BBa_K792008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Tryptophan rich peptide (PolyWb)
creator: Manuel Gim??nez
created: 2012-09-24T11:00:00Z
modified: 2015-05-08T01:13:23Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1047_
m user id: 0
owner id: 11811
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Stable and soluble '''Histidine''' rich peptide (designed in silico) This part has a '''HindIII''' restriction site as extra.
mutable notes: PolyWb was desing taking into acount the following consideration: # avoided repeating the same residue in tandem to minimize local tRNA depleation # avoided Trp in tandem because of therir low solubility # we included Gly to avoid the formation of stable structures # included acidic and basic amino acids to increase solubility Sequence obtained by retro-translation. Codon and mRNA-secondary structure optimized for yeast.
mutable provenance: Designed by BsAs Team 2012
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: HindIII restriction site
sequence annotation: stop
sequence annotation: cds
sequence: https://synbiohub.org/public/igem/BBa_K792008_sequence/1

HindIII restriction site
type: SequenceAnnotation
persistent identity: annotation2196471
display id: annotation2196471
version: 1
top level: PolyWb
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K792008/annotation2196471/range2196471/1

https://synbiohub.org/public/igem/BBa_K792008/annotation2196471/range2196471/1
type: Range
persistent identity: range2196471
display id: range2196471
version: 1
direction: direction/forward
top level: PolyWb
start: 1
end: 6
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2196618
display id: annotation2196618
version: 1
top level: PolyWb
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K792008/annotation2196618/range2196618/1

https://synbiohub.org/public/igem/BBa_K792008/annotation2196618/range2196618/1
type: Range
persistent identity: range2196618
display id: range2196618
version: 1
direction: direction/forward
top level: PolyWb
start: 115
end: 120
orientation: inline

cds
type: SequenceAnnotation
persistent identity: annotation2204264
display id: annotation2204264
version: 1
top level: PolyWb
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K792008/annotation2204264/range2204264/1

https://synbiohub.org/public/igem/BBa_K792008/annotation2204264/range2204264/1
type: Range
persistent identity: range2204264
display id: range2204264
version: 1
direction: direction/forward
top level: PolyWb
start: 7
end: 114
orientation: inline