//proteindomain/internal/special
type: Collection
persistent identity: proteindomain internal special
display id: proteindomain_internal_special
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
owned by: james
owned by: myers
member: S-tag
member: HA
member: BBa_T2028
member: BBa_K112502
member: BBa_T2025
member: HA tag
member: VSV-G tag
member: Strep-tag
member: V5 tag
member: FLAG
member: His
member: S*Tag
member: BBa_K112602
member: TAT-trojan
member: Polyargini
member: 7xHis
member: c-Myc

BBa_T2025
type: ComponentDefinition
persistent identity: BBa T2025
display id: BBa_T2025
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Glutathione S-transferase internal domain
creator: Reshma Shetty
created: 2009-09-16T11:00:00Z
modified: 2015-05-08T01:14:52Z
discontinued: false
dominant: false
group u list: _41_
m user id: 0
owner id: 126
owning group id: 162
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is a GST internal domain designed to be between protein domains. GST domains are a type of affinity domain that can be used to purify and/or measure the quantity of proteins.
mutable notes: Rare codons in common model organisms were avoided along with restriction sites used in various assembly standards.
mutable provenance: This is a synthetic sequence.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: GST
sequence: https://synbiohub.org/public/igem/BBa_T2025_sequence/1

GST
type: SequenceAnnotation
persistent identity: annotation2021346
display id: annotation2021346
version: 1
top level: BBa_T2025
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_T2025/annotation2021346/range2021346/1

https://synbiohub.org/public/igem/BBa_T2025/annotation2021346/range2021346/1
type: Range
persistent identity: range2021346
display id: range2021346
version: 1
direction: direction/forward
top level: BBa_T2025
start: 1
end: 657
orientation: inline

TAT-trojan
type: ComponentDefinition
persistent identity: BBa K792003
display id: BBa_K792003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Import enhancer - HIV TAT penetratin (trojan peptide)
creator: Manuel Gim??nez
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:23Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1047_
m user id: 0
owner id: 11811
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: Trojan peptides are short sequences that penetrate through the plasma membrane inside the cell without the need of any receptor or endocitosis process [Derossi 1998]. They can be used to increase the efficiency with which a protein enters a cell. This part is the penetratin from the HIV TAT protein, and has to be used just before the coding sequence of the peptide that you want to be ''import enhanced''.
mutable notes: Sequence obtained by retro-translation
mutable provenance: HIV TAT penetratin
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Other
role: SO:0000110
sequence annotation: Trojan peptide
sequence annotation: HindIII restriction site
sequence: https://synbiohub.org/public/igem/BBa_K792003_sequence/1

Trojan peptide
type: SequenceAnnotation
persistent identity: annotation2197881
display id: annotation2197881
version: 1
top level: TAT-trojan
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K792003/annotation2197881/range2197881/1

https://synbiohub.org/public/igem/BBa_K792003/annotation2197881/range2197881/1
type: Range
persistent identity: range2197881
display id: range2197881
version: 1
direction: direction/forward
top level: TAT-trojan
start: 7
end: 39
orientation: inline

HindIII restriction site
type: SequenceAnnotation
persistent identity: annotation2196325
display id: annotation2196325
version: 1
top level: TAT-trojan
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K792003/annotation2196325/range2196325/1

https://synbiohub.org/public/igem/BBa_K792003/annotation2196325/range2196325/1
type: Range
persistent identity: range2196325
display id: range2196325
version: 1
direction: direction/forward
top level: TAT-trojan
start: 1
end: 6
orientation: inline

HA
type: ComponentDefinition
persistent identity: BBa K128001
display id: BBa_K128001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: HA affinity tag
creator: Sara Mouradian
created: 2008-07-09T11:00:00Z
modified: 2015-05-08T01:09:46Z
discontinued: false
dominant: false
experience: experience/None
group u list: _178_
m user id: 0
owner id: 2822
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This is the polypeptide protein HA tag. It is used in antibody binding assays if there is no antibody against the studied protein. It should be put at either the N terminus or the C terminus of the protein This part uses the modified Silver BioBrick prefix and suffix to allow for protein construction.
mutable notes: Tags are almost always used in conjunction with another protein (the one being studied), so we used the modified Silver BioBrick prefix and suffix to make the construction of funcitonal fusion peptides possible
mutable provenance: This sequence is easily recognized by many antibodies.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000324
role: partType/Tag
sequence annotation: HA tag
sequence: https://synbiohub.org/public/igem/BBa_K128001_sequence/1

HA tag
type: SequenceAnnotation
persistent identity: annotation1966476
display id: annotation1966476
version: 1
top level: HA
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K128001/annotation1966476/range1966476/1

https://synbiohub.org/public/igem/BBa_K128001/annotation1966476/range1966476/1
type: Range
persistent identity: range1966476
display id: range1966476
version: 1
direction: direction/forward
top level: HA
start: 1
end: 27
orientation: inline

7xHis
type: ComponentDefinition
persistent identity: BBa T2002
display id: BBa_T2002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: 7x His tag special internal domain
creator: Reshma Shetty
created: 2009-08-10T11:00:00Z
modified: 2015-05-08T01:14:52Z
discontinued: false
dominant: false
experience: experience/None
group u list: _41_
m user id: 0
owner id: 126
owning group id: 162
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is a 6x His tag special internal domain is designed to be between protein domains. His tags are a type of affinity tag that can be used to purify and/or measure the quantity of proteins.
mutable notes: Used both Histidine codons to minimize repeats.
mutable provenance: This is a synthetic sequence.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: 7x His
sequence: https://synbiohub.org/public/igem/BBa_T2002_sequence/1

7x His
type: SequenceAnnotation
persistent identity: annotation2016499
display id: annotation2016499
version: 1
top level: 7xHis
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_T2002/annotation2016499/range2016499/1

https://synbiohub.org/public/igem/BBa_T2002/annotation2016499/range2016499/1
type: Range
persistent identity: range2016499
display id: range2016499
version: 1
direction: direction/forward
top level: 7xHis
start: 1
end: 21
orientation: inline

BBa_T2028
type: ComponentDefinition
persistent identity: BBa T2028
display id: BBa_T2028
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Chitin binding internal domain
creator: Reshma Shetty
created: 2009-09-16T11:00:00Z
modified: 2015-05-08T01:14:52Z
discontinued: false
dominant: false
group u list: _41_
m user id: 0
owner id: 126
owning group id: 162
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is a chitin binding domain (CBD) designed to be at between protein domains. Chitin binding domains are a type of affinity tag that can be used to purify proteins.
mutable notes: Rare codons from common model organisms as well as restriction sites used in various assembly standards were removed.
mutable provenance: This is a synthetic sequence.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: chitin binding domain
sequence: https://synbiohub.org/public/igem/BBa_T2028_sequence/1

chitin binding domain
type: SequenceAnnotation
persistent identity: annotation2021347
display id: annotation2021347
version: 1
top level: BBa_T2028
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_T2028/annotation2021347/range2021347/1

https://synbiohub.org/public/igem/BBa_T2028/annotation2021347/range2021347/1
type: Range
persistent identity: range2021347
display id: range2021347
version: 1
direction: direction/forward
top level: BBa_T2028
start: 1
end: 156
orientation: inline

FLAG
type: ComponentDefinition
persistent identity: BBa T2005
display id: BBa_T2005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: FLAG octapeptide special internal domain (DYKDDDDK)
creator: Reshma Shetty
created: 2009-08-10T11:00:00Z
modified: 2015-05-08T01:14:52Z
discontinued: false
dominant: false
experience: experience/None
group u list: _41_
m user id: 0
owner id: 126
owning group id: 162
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is a FLAG tag octapeptide special internal domain designed to be internal to the sequence of a protein. FLAG tags are a type of affinity tag that can be used to purify and/or measure the quantity of proteins.
mutable notes: The FLAG sequence AspTyrLysAspAspAspAspLys (DYKDDDDK) is one of the most commonly used FLAG tag sequences. Each of the amino acids in this octapeptide have two possible codon choices. For each amino acid, neither of the two codon choices appear to be rare in the common model organisms. So codons were selected to minimize repeats.
mutable provenance: This is a synthetic sequence.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: FLAG octapeptide
sequence: https://synbiohub.org/public/igem/BBa_T2005_sequence/1

FLAG octapeptide
type: SequenceAnnotation
persistent identity: annotation2017176
display id: annotation2017176
version: 1
top level: FLAG
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_T2005/annotation2017176/range2017176/1

https://synbiohub.org/public/igem/BBa_T2005/annotation2017176/range2017176/1
type: Range
persistent identity: range2017176
display id: range2017176
version: 1
direction: direction/forward
top level: FLAG
start: 1
end: 24
orientation: inline

Strep-tag
type: ComponentDefinition
persistent identity: BBa T2014
display id: BBa_T2014
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Strep-tag II special internal domain (WSHPQFEK)
creator: Reshma Shetty
created: 2009-08-12T11:00:00Z
modified: 2015-05-08T01:14:52Z
discontinued: false
dominant: false
experience: experience/None
group u list: _41_
m user id: 0
owner id: 126
owning group id: 162
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is a Strep tag tail domain designed to be internal to a protein. Strep tags are typically used for detection, purification and immobilization of proteins.
mutable notes: This domain encodes the Strep tag sequence WRHPQFGG. The codons at each amino acid position were selected to avoid rare codons as much as possible in organisms like ''E. coli'', humans, mouse, ''B. subtilis'', ''C. elegans'', ''Streptomyces coelicolor'' and other common model organisms and/or industrial hosts. Another factor in codon selection was avoiding avoid repeat sequences.
mutable provenance: This is a synthetic sequence.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: Strep-tag II
sequence: https://synbiohub.org/public/igem/BBa_T2014_sequence/1

Strep-tag II
type: SequenceAnnotation
persistent identity: annotation2017312
display id: annotation2017312
version: 1
top level: Strep-tag
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_T2014/annotation2017312/range2017312/1

https://synbiohub.org/public/igem/BBa_T2014/annotation2017312/range2017312/1
type: Range
persistent identity: range2017312
display id: range2017312
version: 1
direction: direction/forward
top level: Strep-tag
start: 1
end: 24
orientation: inline

S*Tag
type: ComponentDefinition
persistent identity: BBa J32017
display id: BBa_J32017
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: S-Tag
creator: Austen Heinz
created: 2006-08-24T11:00:00Z
modified: 2015-08-31T04:08:46Z
discontinued: false
dominant: false
experience: experience/None
group u list: _50_
m user id: 0
owner id: 495
owning group id: 50
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: The S???Tag sequence is a novel fusion peptide tag for recombinant proteins that allows detection by a rapid, sensitive homogeneous assay or by colorimetric detection in Western blots. Proteins can also be rapidly purified from crude extracts using a one step affinity separation method. The system is based on the strong interaction between the 15aa S???Tag and 103aa S-protein.
mutable notes: .
mutable provenance: Novagen
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000324
role: partType/Tag
sequence annotation: S-Tag
sequence: https://synbiohub.org/public/igem/BBa_J32017_sequence/1

S-Tag
type: SequenceAnnotation
persistent identity: annotation1898184
display id: annotation1898184
version: 1
top level: S*Tag
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_J32017/annotation1898184/range1898184/1

https://synbiohub.org/public/igem/BBa_J32017/annotation1898184/range1898184/1
type: Range
persistent identity: range1898184
display id: range1898184
version: 1
direction: direction/forward
top level: S*Tag
start: 1
end: 46
orientation: inline

S-tag
type: ComponentDefinition
persistent identity: BBa T2017
display id: BBa_T2017
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: S-tag special internal domain (KETAAAKFERQHMDS)
creator: Reshma Shetty
created: 2009-09-02T11:00:00Z
modified: 2015-05-08T01:14:52Z
discontinued: false
dominant: false
experience: experience/None
group u list: _41_
m user id: 0
owner id: 126
owning group id: 162
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is a S-tag special internal domain designed to between protein domains. S-tags are a type of affinity tag that can be used to purify and/or measure the quantity of proteins.
mutable notes: Rare codons from various model organisms were avoided where possible.
mutable provenance: This is a synthetic sequence.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: S-tag
sequence: https://synbiohub.org/public/igem/BBa_T2017_sequence/1

S-tag
type: SequenceAnnotation
persistent identity: annotation2018274
display id: annotation2018274
version: 1
top level: S-tag
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_T2017/annotation2018274/range2018274/1

https://synbiohub.org/public/igem/BBa_T2017/annotation2018274/range2018274/1
type: Range
persistent identity: range2018274
display id: range2018274
version: 1
direction: direction/forward
top level: S-tag
start: 1
end: 45
orientation: inline

HA tag
type: ComponentDefinition
persistent identity: BBa K112506
display id: BBa_K112506
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: < HA > in BBb
creator: Sherine Cheung
created: 2008-10-21T11:00:00Z
modified: 2015-05-08T01:09:18Z
discontinued: false
dominant: false
experience: experience/None
group u list: _224_
m user id: 0
owner id: 3386
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This is an HA tag without a start or a stop codon. It is in BBb format. ---- This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at: [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
mutable notes: N/A
mutable provenance: Construction of {<HA>} basic part K112506 Wobble PCR of cc001/cc010 ( 51 bp, EcoRI/BamHI) Sub into pBca1256 (EcoRI/BamHI, 2227+ 910,L) Product is pBca1256-K112506 -------------------------------------------- cc001 Forward Biobricking of {<HA>} CgATAgaattcATGagatcttacccatacgacgtcccagac cc010 Reverse biobricking of {<HA>} ccagtggatcccccagcgtagtctgggacgtcgtatggg
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000324
role: partType/Tag
sequence: https://synbiohub.org/public/igem/BBa_K112506_sequence/1

VSV-G tag
type: ComponentDefinition
persistent identity: BBa T2022
display id: BBa_T2022
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: VSV-G epitope tag special internal domain (YTDIEMNRLGK)
creator: Reshma Shetty
created: 2009-09-02T11:00:00Z
modified: 2015-05-08T01:14:52Z
discontinued: false
dominant: false
experience: experience/None
group u list: _41_
m user id: 0
owner id: 126
owning group id: 162
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is a VSV-G epitope tag special internal domain designed to be used between protein domains. VSV-G tags are a type of epitope tag that can be used to measure the quantity of proteins.
mutable notes: Rare codons from model organisms were avoided where possible.
mutable provenance: This is a synthetic sequence.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: VSV-G tag
sequence: https://synbiohub.org/public/igem/BBa_T2022_sequence/1

VSV-G tag
type: SequenceAnnotation
persistent identity: annotation2018283
display id: annotation2018283
version: 1
top level: VSV-G tag
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_T2022/annotation2018283/range2018283/1

https://synbiohub.org/public/igem/BBa_T2022/annotation2018283/range2018283/1
type: Range
persistent identity: range2018283
display id: range2018283
version: 1
direction: direction/forward
top level: VSV-G tag
start: 1
end: 33
orientation: inline

HA tag
type: ComponentDefinition
persistent identity: BBa T2011
display id: BBa_T2011
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: hemagglutinin (HA) tag special internal domain (YPYDVPDYA)
creator: Reshma Shetty
created: 2009-08-10T11:00:00Z
modified: 2015-05-08T01:14:52Z
discontinued: false
dominant: false
experience: experience/None
group u list: _41_
m user id: 0
owner id: 126
owning group id: 162
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is a HA tag special internal domain designed to be within a protein. HA tags are a type of affinity tag that can be used to purify and/or measure the quantity of proteins.
mutable notes: This domain encodes the HA sequence YPYDVPDYA. The codons at each amino acid position were selected to avoid rare codons as much as possible in organisms like ''E. coli'', humans, mouse, ''B. subtilis'', C. elegans, ''Streptomyces coelicolor'' and other common model organisms and/or industrial hosts. Another factor in codon selection was avoiding avoid repeat sequences.
mutable provenance: This is a synthetic sequence.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: HA tag
sequence: https://synbiohub.org/public/igem/BBa_T2011_sequence/1

HA tag
type: SequenceAnnotation
persistent identity: annotation2017186
display id: annotation2017186
version: 1
top level: HA tag
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_T2011/annotation2017186/range2017186/1

https://synbiohub.org/public/igem/BBa_T2011/annotation2017186/range2017186/1
type: Range
persistent identity: range2017186
display id: range2017186
version: 1
direction: direction/forward
top level: HA tag
start: 1
end: 27
orientation: inline

BBa_K112602
type: ComponentDefinition
persistent identity: BBa K112602
display id: BBa_K112602
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: < FLAG tag > in BBb
creator: Sherine Cheung
created: 2008-09-02T11:00:00Z
modified: 2015-05-08T01:09:18Z
discontinued: false
dominant: true
experience: experience/None
group u list: _224_
m user id: 0
owner id: 3386
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This is FLAG tag with no start and no stop codon ---- This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at: [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
mutable notes: N/A
mutable provenance: Construction of {<FLAG>} basic part K112602 Wobble PCR SC004/SC005 (45 bp, EcoRI/BamHI) Sub into pBca1256 (EcoRI/BamHI, 2472+ 910) Product is pBca1256-K112602 --------------------------------------------------- SC004 Forward Biobricking of <FLAG-tag> cgataGAATTCatgAGATCTgactacaaggatgacgacg SC005 Reverse Biobricking of <FLAG-tag> ccagtGGATCCcttgtcgtcgtcatccttgtagtc
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000324
role: partType/Tag
sequence: https://synbiohub.org/public/igem/BBa_K112602_sequence/1

V5 tag
type: ComponentDefinition
persistent identity: BBa T2020
display id: BBa_T2020
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: V5 epitope tag special internal domain (GKPIPNPLLGLDST)
creator: Reshma Shetty
created: 2009-09-02T11:00:00Z
modified: 2015-05-08T01:14:52Z
discontinued: false
dominant: false
experience: experience/None
group u list: _41_
m user id: 0
owner id: 126
owning group id: 162
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is a V5-tag special internal domain designed to between protein domains. V5 tags are a type of epitope tag that can be used to measure the quantity of proteins.
mutable notes: Rare codons from common model organisms were avoided.
mutable provenance: This is a synthetic sequence.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: V5 epitope tag
sequence: https://synbiohub.org/public/igem/BBa_T2020_sequence/1

V5 epitope tag
type: SequenceAnnotation
persistent identity: annotation2018280
display id: annotation2018280
version: 1
top level: V5 tag
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_T2020/annotation2018280/range2018280/1

https://synbiohub.org/public/igem/BBa_T2020/annotation2018280/range2018280/1
type: Range
persistent identity: range2018280
display id: range2018280
version: 1
direction: direction/forward
top level: V5 tag
start: 1
end: 42
orientation: inline

BBa_K112502
type: ComponentDefinition
persistent identity: BBa K112502
display id: BBa_K112502
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: < myc > in BBb
creator: Sherine Cheung
created: 2008-10-21T11:00:00Z
modified: 2015-05-08T01:09:18Z
discontinued: false
dominant: true
experience: experience/None
group u list: _224_
m user id: 0
owner id: 3386
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This is a myc tag without a stop or a stop codon. It is in BBb format. ---- This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at: [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
mutable notes: N/A
mutable provenance: Construction of {<myc>} basic part K112502 Wobble PCR of cc004/cc005 ( 51 bp, EcoRI/BamHI) Sub into pBca1256 (EcoRI/BamHI, 2227+ 910,L) Product is pBca1256-K112502 -------------------------------------------- cc004 Forward Biobricking of {<myc>} CgATAgaattcATGagatctGAACAAAAACTCATCTCAGAAGAGG cc005 Reverse Biobricking of {<myc>} ccagtggatccCAGATCCTCTTCTGAGATGAGTTTTTG
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000324
role: partType/Tag
sequence: https://synbiohub.org/public/igem/BBa_K112502_sequence/1

His
type: ComponentDefinition
persistent identity: BBa K128005
display id: BBa_K128005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: His affinity tag
creator: Prarthna Desai
created: 2008-10-24T11:00:00Z
modified: 2015-05-08T01:09:46Z
discontinued: false
dominant: false
experience: experience/None
group u list: _178_
m user id: 0
owner id: 2826
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This codes for six Histidine residues to allow for His column protein purification. It will work best if put at either the N or C terminus of your protein construct. This part uses the modified Silver BioBrick prefix and suffix to allow for protein construction.
mutable notes: This part uses the modified Silver BioBrick prefix and suffix to allow for protein construction.
mutable provenance: This sequence is easily recognized by many antibodies.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000324
role: partType/Tag
sequence: https://synbiohub.org/public/igem/BBa_K128005_sequence/1

FLAG
type: ComponentDefinition
persistent identity: BBa K128000
display id: BBa_K128000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: FLAG affinity tag
creator: Sara Mouradian
created: 2008-07-09T11:00:00Z
modified: 2015-05-08T01:09:46Z
discontinued: false
dominant: false
experience: experience/None
group u list: _178_
m user id: 0
owner id: 2822
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This is the polypeptide protein FLAG tag. It is used in antibody binding assays if there is no antibody against the studied protein. It should be put at either the N terminus or the C terminus of the protein This part uses the modified Silver BioBrick prefix and suffix to allow for protein construction.
mutable notes: Tags are almost always used in conjunction with another protein (the one being studied), so we used the modified Silver BioBrick prefix and suffix to make the construction of funcitonal fusion peptides possible
mutable provenance: This sequence is easily recognized by many antibodies.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000324
role: partType/Tag
sequence annotation: Flag tag
sequence: https://synbiohub.org/public/igem/BBa_K128000_sequence/1

Flag tag
type: SequenceAnnotation
persistent identity: annotation1966459
display id: annotation1966459
version: 1
top level: FLAG
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K128000/annotation1966459/range1966459/1

https://synbiohub.org/public/igem/BBa_K128000/annotation1966459/range1966459/1
type: Range
persistent identity: range1966459
display id: range1966459
version: 1
direction: direction/forward
top level: FLAG
start: 1
end: 25
orientation: inline

Polyargini
type: ComponentDefinition
persistent identity: BBa K792004
display id: BBa_K792004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Import enhancer - Polyarginine (trojan peptide)
creator: Manuel Gim??nez
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:23Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1047_
m user id: 0
owner id: 11811
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Trojan peptides are short sequences that penetrate through the plasma membrane inside the cell without the need of any receptor or endocitosis process [Derossi 1998]. They can be used to increase the efficiency with which a protein enters a cell. This part is Polyarginine, and has to be used just before the coding sequence of the peptide that you want to be import enhanced. (refer to ''Characterisation of cell-penetrating peptide-mediated peptide delivery'' [Jones et al 2005] for more information about polyarginine)
mutable notes: Sequence obtained by retro-translation. Codon and mRNA-secondary structure optimized for yeast.
mutable provenance: Characterisation of cell-penetrating peptide-mediated peptide delivery [Jones et al 2005]
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Other
role: SO:0000110
sequence annotation: Trojan peptide
sequence annotation: HindIII restriction site
sequence: https://synbiohub.org/public/igem/BBa_K792004_sequence/1

Trojan peptide
type: SequenceAnnotation
persistent identity: annotation2197880
display id: annotation2197880
version: 1
top level: Polyargini
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K792004/annotation2197880/range2197880/1

https://synbiohub.org/public/igem/BBa_K792004/annotation2197880/range2197880/1
type: Range
persistent identity: range2197880
display id: range2197880
version: 1
direction: direction/forward
top level: Polyargini
start: 7
end: 39
orientation: inline

HindIII restriction site
type: SequenceAnnotation
persistent identity: annotation2196615
display id: annotation2196615
version: 1
top level: Polyargini
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K792004/annotation2196615/range2196615/1

https://synbiohub.org/public/igem/BBa_K792004/annotation2196615/range2196615/1
type: Range
persistent identity: range2196615
display id: range2196615
version: 1
direction: direction/forward
top level: Polyargini
start: 1
end: 6
orientation: inline

c-Myc
type: ComponentDefinition
persistent identity: BBa T2008
display id: BBa_T2008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: c-Myc special internal domain (EQKLISEEDL)
creator: Reshma Shetty
created: 2009-08-10T11:00:00Z
modified: 2015-05-08T01:14:52Z
discontinued: false
dominant: false
experience: experience/None
group u list: _41_
m user id: 0
owner id: 126
owning group id: 162
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is a c-Myc tag special internal domain designed to be within a protein. c-Myc tags are a type of affinity tag that can be used to purify and/or measure the quantity of proteins.
mutable notes: This domain encodes the c-Myc sequence EQKLISEEDL. The codons at each amino acid position were selected to avoid rare codons in organisms like E. coli, humans, mouse, B. subtilis, C. elegans, Streptomyces coelicolor and other common model organisms and/or industrial hosts.
mutable provenance: This is a synthetic sequence.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: c-Myc tag
sequence: https://synbiohub.org/public/igem/BBa_T2008_sequence/1

c-Myc tag
type: SequenceAnnotation
persistent identity: annotation2017181
display id: annotation2017181
version: 1
top level: c-Myc
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_T2008/annotation2017181/range2017181/1

https://synbiohub.org/public/igem/BBa_T2008/annotation2017181/range2017181/1
type: Range
persistent identity: range2017181
display id: range2017181
version: 1
direction: direction/forward
top level: c-Myc
start: 1
end: 30
orientation: inline