//proteindomain/internal/special
type: Collection
persistent identity: proteindomain internal special
display id: proteindomain_internal_special
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
owned by: james
owned by: myers
member: https://synbiohub.org/public/igem/BBa_T2017/1
member: https://synbiohub.org/public/igem/BBa_K128001/1
member: https://synbiohub.org/public/igem/BBa_T2028/1
member: https://synbiohub.org/public/igem/BBa_K112502/1
member: https://synbiohub.org/public/igem/BBa_T2025/1
member: https://synbiohub.org/public/igem/BBa_K112506/1
member: https://synbiohub.org/public/igem/BBa_T2022/1
member: https://synbiohub.org/public/igem/BBa_T2014/1
member: https://synbiohub.org/public/igem/BBa_T2020/1
member: https://synbiohub.org/public/igem/BBa_T2005/1
member: https://synbiohub.org/public/igem/BBa_K128005/1
member: https://synbiohub.org/public/igem/BBa_J32017/1
member: https://synbiohub.org/public/igem/BBa_K112602/1
member: https://synbiohub.org/public/igem/BBa_T2011/1
member: https://synbiohub.org/public/igem/BBa_K128000/1
member: https://synbiohub.org/public/igem/BBa_K792003/1
member: https://synbiohub.org/public/igem/BBa_K792004/1
member: https://synbiohub.org/public/igem/BBa_T2002/1
member: https://synbiohub.org/public/igem/BBa_T2008/1

//proteindomain/internal
type: Collection
persistent identity: proteindomain internal
display id: proteindomain_internal
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
owned by: james
owned by: myers
member: //proteindomain/internal/special
member: CspB | RFP
member: BBa_K525010
member: SP-Lipocal
member: BBa_K525001
member: BBa_K525560
member: SP-AntiNIP
member: SgsE | RFP
member: SgsE | Luc
member: Tm-betaLac
member: BBa_K157002
member: BBa_K157007
member: BBa_K525008
member: SbpA | RFP
member: Tm-Splitlu
member: Tm-Fluolin
member: SgsE
member: BisdAB
member: SgsE | CFP
member: cspB
member: Luc
member: BBa_K157004
member: SgsE|LigA
member: SbpA
member: BBa_J70620
member: Tm-nCFP
member: BBa_K1486052
member: BBa_K157005
member: LigA
member: SbpA|LigA
member: SgsE | Red
member: BBa_K525582
member: BBa_K157006
member: Tm-nYFP
member: BBa_K525007
member: SbpA | Red
member: SgsE | YFP
member: BBa_K157003
member: Red
member: BBa_K525009
member: SbpA | YFP
member: Tm-Split-L
member: EnvZ Tail
member: BBa_K157008
member: BBa_K525562
member: SbpA | CFP
member: BBa_K1909004

Tm-nYFP
type: ComponentDefinition
persistent identity: BBa K157015
display id: BBa_K157015
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Tm-nYFP
creator: iGEM Team Freiburg 2008
created: 2008-10-25T11:00:00Z
modified: 2015-05-08T01:10:54Z
discontinued: false
dominant: false
experience: experience/None
group u list: _232_
m user id: 0
owner id: 1675
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: N-terminal part of the Split-flourophore YFP, fused to the trans-membrane region of the Erbb1/EGF-receptor.
mutable notes: none
mutable provenance: Derived by cloning the parts BBa_K157002, BBa_K157008. For further information see the description of the parts mentioned above.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: N-Term_YFP
sequence annotation: NgoMI
sequence annotation: Tm
sequence annotation: Scar (Thr, Gly)
sequence annotation: Tm-nYFP
sequence annotation: AgeI
sequence: https://synbiohub.org/public/igem/BBa_K157015_sequence/1

N-Term_YFP
type: SequenceAnnotation
persistent identity: annotation1991279
display id: annotation1991279
version: 1
top level: Tm-nYFP
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157015/annotation1991279/range1991279/1

https://synbiohub.org/public/igem/BBa_K157015/annotation1991279/range1991279/1
type: Range
persistent identity: range1991279
display id: range1991279
version: 1
direction: direction/forward
top level: Tm-nYFP
start: 84
end: 552
orientation: inline

NgoMI
type: SequenceAnnotation
persistent identity: annotation1989841
display id: annotation1989841
version: 1
top level: Tm-nYFP
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157015/annotation1989841/range1989841/1

https://synbiohub.org/public/igem/BBa_K157015/annotation1989841/range1989841/1
type: Range
persistent identity: range1989841
display id: range1989841
version: 1
direction: direction/forward
top level: Tm-nYFP
start: 4
end: 9
orientation: inline

Tm
type: SequenceAnnotation
persistent identity: annotation1991277
display id: annotation1991277
version: 1
top level: Tm-nYFP
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157015/annotation1991277/range1991277/1

https://synbiohub.org/public/igem/BBa_K157015/annotation1991277/range1991277/1
type: Range
persistent identity: range1991277
display id: range1991277
version: 1
direction: direction/forward
top level: Tm-nYFP
start: 10
end: 78
orientation: inline

Scar (Thr, Gly)
type: SequenceAnnotation
persistent identity: annotation1991278
display id: annotation1991278
version: 1
top level: Tm-nYFP
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157015/annotation1991278/range1991278/1

https://synbiohub.org/public/igem/BBa_K157015/annotation1991278/range1991278/1
type: Range
persistent identity: range1991278
display id: range1991278
version: 1
direction: direction/forward
top level: Tm-nYFP
start: 79
end: 84
orientation: inline

Tm-nYFP
type: SequenceAnnotation
persistent identity: annotation1989844
display id: annotation1989844
version: 1
top level: Tm-nYFP
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K157015/annotation1989844/range1989844/1

https://synbiohub.org/public/igem/BBa_K157015/annotation1989844/range1989844/1
type: Range
persistent identity: range1989844
display id: range1989844
version: 1
direction: direction/forward
top level: Tm-nYFP
start: 1
end: 558
orientation: inline

AgeI
type: SequenceAnnotation
persistent identity: annotation1989842
display id: annotation1989842
version: 1
top level: Tm-nYFP
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157015/annotation1989842/range1989842/1

https://synbiohub.org/public/igem/BBa_K157015/annotation1989842/range1989842/1
type: Range
persistent identity: range1989842
display id: range1989842
version: 1
direction: direction/forward
top level: Tm-nYFP
start: 553
end: 558
orientation: inline

SbpA | YFP
type: ComponentDefinition
persistent identity: BBa K525405
display id: BBa_K525405
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Fusion Protein of S-Layer SbpA and mCitrine
creator: Timo Wolf
created: 2011-09-09T11:00:00Z
modified: 2016-01-26T10:05:40Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _690_
m user id: 4206
owner id: 6668
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Fusion Protein of S-Layer sbpA and mCitrine
mutable notes: Fusion Protein of BBa_K525403 and BBa_J18931
mutable provenance: Fusion Protein of S-Layer sbpA and mCitrine
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: T7 promoter
sequence annotation: SbpA domain
sequence annotation: Freiburg scar
sequence annotation: RBS
sequence annotation: NgoMIV
sequence annotation: K525401
sequence annotation: lac operator
sequence annotation: AgeI
sequence annotation: mCitrine (YFP)
sequence annotation: B0034
sequence annotation: TAA
sequence annotation: ATG
sequence annotation: J18931
sequence: https://synbiohub.org/public/igem/BBa_K525405_sequence/1

T7 promoter
type: SequenceAnnotation
persistent identity: annotation2127654
display id: annotation2127654
version: 1
top level: SbpA | YFP
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K525405/annotation2127654/range2127654/1

https://synbiohub.org/public/igem/BBa_K525405/annotation2127654/range2127654/1
type: Range
persistent identity: range2127654
display id: range2127654
version: 1
direction: direction/forward
top level: SbpA | YFP
start: 1
end: 20
orientation: inline

SbpA domain
type: SequenceAnnotation
persistent identity: annotation2127664
display id: annotation2127664
version: 1
top level: SbpA | YFP
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K525405/annotation2127664/range2127664/1

https://synbiohub.org/public/igem/BBa_K525405/annotation2127664/range2127664/1
type: Range
persistent identity: range2127664
display id: range2127664
version: 1
direction: direction/forward
top level: SbpA | YFP
start: 73
end: 3192
orientation: inline

Freiburg scar
type: SequenceAnnotation
persistent identity: annotation2127665
display id: annotation2127665
version: 1
top level: SbpA | YFP
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K525405/annotation2127665/range2127665/1

https://synbiohub.org/public/igem/BBa_K525405/annotation2127665/range2127665/1
type: Range
persistent identity: range2127665
display id: range2127665
version: 1
direction: direction/forward
top level: SbpA | YFP
start: 3193
end: 3198
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2127657
display id: annotation2127657
version: 1
top level: SbpA | YFP
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K525405/annotation2127657/range2127657/1

https://synbiohub.org/public/igem/BBa_K525405/annotation2127657/range2127657/1
type: Range
persistent identity: range2127657
display id: range2127657
version: 1
direction: direction/forward
top level: SbpA | YFP
start: 56
end: 59
orientation: inline

NgoMIV
type: SequenceAnnotation
persistent identity: annotation2127660
display id: annotation2127660
version: 1
top level: SbpA | YFP
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K525405/annotation2127660/range2127660/1

https://synbiohub.org/public/igem/BBa_K525405/annotation2127660/range2127660/1
type: Range
persistent identity: range2127660
display id: range2127660
version: 1
direction: direction/forward
top level: SbpA | YFP
start: 76
end: 81
orientation: inline

K525401
type: SequenceAnnotation
persistent identity: annotation2127663
display id: annotation2127663
version: 1
top level: SbpA | YFP
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K525405/annotation2127663/range2127663/1

https://synbiohub.org/public/igem/BBa_K525405/annotation2127663/range2127663/1
type: Range
persistent identity: range2127663
display id: range2127663
version: 1
direction: direction/forward
top level: SbpA | YFP
start: 73
end: 3192
orientation: inline

lac operator
type: SequenceAnnotation
persistent identity: annotation2127655
display id: annotation2127655
version: 1
top level: SbpA | YFP
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K525405/annotation2127655/range2127655/1

https://synbiohub.org/public/igem/BBa_K525405/annotation2127655/range2127655/1
type: Range
persistent identity: range2127655
display id: range2127655
version: 1
direction: direction/forward
top level: SbpA | YFP
start: 20
end: 44
orientation: inline

AgeI
type: SequenceAnnotation
persistent identity: annotation2127662
display id: annotation2127662
version: 1
top level: SbpA | YFP
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K525405/annotation2127662/range2127662/1

https://synbiohub.org/public/igem/BBa_K525405/annotation2127662/range2127662/1
type: Range
persistent identity: range2127662
display id: range2127662
version: 1
direction: direction/forward
top level: SbpA | YFP
start: 3913
end: 3918
orientation: inline

mCitrine (YFP)
type: SequenceAnnotation
persistent identity: annotation2127666
display id: annotation2127666
version: 1
top level: SbpA | YFP
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K525405/annotation2127666/range2127666/1

https://synbiohub.org/public/igem/BBa_K525405/annotation2127666/range2127666/1
type: Range
persistent identity: range2127666
display id: range2127666
version: 1
direction: direction/forward
top level: SbpA | YFP
start: 3199
end: 3921
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation2127656
display id: annotation2127656
version: 1
top level: SbpA | YFP
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K525405/annotation2127656/range2127656/1

https://synbiohub.org/public/igem/BBa_K525405/annotation2127656/range2127656/1
type: Range
persistent identity: range2127656
display id: range2127656
version: 1
direction: direction/forward
top level: SbpA | YFP
start: 53
end: 64
orientation: inline

TAA
type: SequenceAnnotation
persistent identity: annotation2127661
display id: annotation2127661
version: 1
top level: SbpA | YFP
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K525405/annotation2127661/range2127661/1

https://synbiohub.org/public/igem/BBa_K525405/annotation2127661/range2127661/1
type: Range
persistent identity: range2127661
display id: range2127661
version: 1
direction: direction/forward
top level: SbpA | YFP
start: 3919
end: 3921
orientation: inline

ATG
type: SequenceAnnotation
persistent identity: annotation2127658
display id: annotation2127658
version: 1
top level: SbpA | YFP
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K525405/annotation2127658/range2127658/1

https://synbiohub.org/public/igem/BBa_K525405/annotation2127658/range2127658/1
type: Range
persistent identity: range2127658
display id: range2127658
version: 1
direction: direction/forward
top level: SbpA | YFP
start: 73
end: 75
orientation: inline

J18931
type: SequenceAnnotation
persistent identity: annotation2127667
display id: annotation2127667
version: 1
top level: SbpA | YFP
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K525405/annotation2127667/range2127667/1

https://synbiohub.org/public/igem/BBa_K525405/annotation2127667/range2127667/1
type: Range
persistent identity: range2127667
display id: range2127667
version: 1
direction: direction/forward
top level: SbpA | YFP
start: 3199
end: 3921
orientation: inline

BBa_K525560
type: ComponentDefinition
persistent identity: BBa K525560
display id: BBa_K525560
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Fusion protein of NADP+ Oxidoreductase and BisdA and BisdB
creator: Anna Drong
created: 2011-09-10T11:00:00Z
modified: 2015-05-08T01:12:34Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _690_
m user id: 0
owner id: 8543
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Fusion protein of NADP+ Oxidoreduktase and BisdA and BisdB; RFC 25
mutable notes: Assembled
mutable provenance: BBa_K525499 and BBa_K123000 and BBa_K123001
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: BisdB
sequence annotation: NgoMIV
sequence annotation: K123000
sequence annotation: BisdA
sequence annotation: Reductase
sequence annotation: K123001
sequence annotation: ATG
sequence annotation: K525499
sequence annotation: Freiburg Scar
sequence annotation: AgeI
sequence annotation: Scar
sequence annotation: TAA
sequence: https://synbiohub.org/public/igem/BBa_K525560_sequence/1

BisdB
type: SequenceAnnotation
persistent identity: annotation2161134
display id: annotation2161134
version: 1
top level: BBa_K525560
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K525560/annotation2161134/range2161134/1

https://synbiohub.org/public/igem/BBa_K525560/annotation2161134/range2161134/1
type: Range
persistent identity: range2161134
display id: range2161134
version: 1
direction: direction/forward
top level: BBa_K525560
start: 1075
end: 2349
orientation: inline

NgoMIV
type: SequenceAnnotation
persistent identity: annotation2161129
display id: annotation2161129
version: 1
top level: BBa_K525560
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K525560/annotation2161129/range2161129/1

https://synbiohub.org/public/igem/BBa_K525560/annotation2161129/range2161129/1
type: Range
persistent identity: range2161129
display id: range2161129
version: 1
direction: direction/forward
top level: BBa_K525560
start: 4
end: 9
orientation: inline

K123000
type: SequenceAnnotation
persistent identity: annotation2161131
display id: annotation2161131
version: 1
top level: BBa_K525560
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K525560/annotation2161131/range2161131/1

https://synbiohub.org/public/igem/BBa_K525560/annotation2161131/range2161131/1
type: Range
persistent identity: range2161131
display id: range2161131
version: 1
direction: direction/forward
top level: BBa_K525560
start: 757
end: 1068
orientation: inline

BisdA
type: SequenceAnnotation
persistent identity: annotation2161132
display id: annotation2161132
version: 1
top level: BBa_K525560
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K525560/annotation2161132/range2161132/1

https://synbiohub.org/public/igem/BBa_K525560/annotation2161132/range2161132/1
type: Range
persistent identity: range2161132
display id: range2161132
version: 1
direction: direction/forward
top level: BBa_K525560
start: 757
end: 1068
orientation: inline

Reductase
type: SequenceAnnotation
persistent identity: annotation2161126
display id: annotation2161126
version: 1
top level: BBa_K525560
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K525560/annotation2161126/range2161126/1

https://synbiohub.org/public/igem/BBa_K525560/annotation2161126/range2161126/1
type: Range
persistent identity: range2161126
display id: range2161126
version: 1
direction: direction/forward
top level: BBa_K525560
start: 1
end: 750
orientation: inline

K123001
type: SequenceAnnotation
persistent identity: annotation2161135
display id: annotation2161135
version: 1
top level: BBa_K525560
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K525560/annotation2161135/range2161135/1

https://synbiohub.org/public/igem/BBa_K525560/annotation2161135/range2161135/1
type: Range
persistent identity: range2161135
display id: range2161135
version: 1
direction: direction/forward
top level: BBa_K525560
start: 1075
end: 2349
orientation: inline

ATG
type: SequenceAnnotation
persistent identity: annotation2161128
display id: annotation2161128
version: 1
top level: BBa_K525560
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K525560/annotation2161128/range2161128/1

https://synbiohub.org/public/igem/BBa_K525560/annotation2161128/range2161128/1
type: Range
persistent identity: range2161128
display id: range2161128
version: 1
direction: direction/forward
top level: BBa_K525560
start: 1
end: 3
orientation: inline

K525499
type: SequenceAnnotation
persistent identity: annotation2161127
display id: annotation2161127
version: 1
top level: BBa_K525560
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K525560/annotation2161127/range2161127/1

https://synbiohub.org/public/igem/BBa_K525560/annotation2161127/range2161127/1
type: Range
persistent identity: range2161127
display id: range2161127
version: 1
direction: direction/forward
top level: BBa_K525560
start: 1
end: 750
orientation: inline

Freiburg Scar
type: SequenceAnnotation
persistent identity: annotation2161133
display id: annotation2161133
version: 1
top level: BBa_K525560
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K525560/annotation2161133/range2161133/1

https://synbiohub.org/public/igem/BBa_K525560/annotation2161133/range2161133/1
type: Range
persistent identity: range2161133
display id: range2161133
version: 1
direction: direction/forward
top level: BBa_K525560
start: 1069
end: 1074
orientation: inline

AgeI
type: SequenceAnnotation
persistent identity: annotation2161136
display id: annotation2161136
version: 1
top level: BBa_K525560
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K525560/annotation2161136/range2161136/1

https://synbiohub.org/public/igem/BBa_K525560/annotation2161136/range2161136/1
type: Range
persistent identity: range2161136
display id: range2161136
version: 1
direction: direction/forward
top level: BBa_K525560
start: 2341
end: 2346
orientation: inline

Scar
type: SequenceAnnotation
persistent identity: annotation2161130
display id: annotation2161130
version: 1
top level: BBa_K525560
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K525560/annotation2161130/range2161130/1

https://synbiohub.org/public/igem/BBa_K525560/annotation2161130/range2161130/1
type: Range
persistent identity: range2161130
display id: range2161130
version: 1
direction: direction/forward
top level: BBa_K525560
start: 751
end: 756
orientation: inline

TAA
type: SequenceAnnotation
persistent identity: annotation2161137
display id: annotation2161137
version: 1
top level: BBa_K525560
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K525560/annotation2161137/range2161137/1

https://synbiohub.org/public/igem/BBa_K525560/annotation2161137/range2161137/1
type: Range
persistent identity: range2161137
display id: range2161137
version: 1
direction: direction/forward
top level: BBa_K525560
start: 2347
end: 2349
orientation: inline

BBa_K157008
type: ComponentDefinition
persistent identity: BBa K157008
display id: BBa_K157008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Split-Venus-nYFP
creator: iGEM Team Freiburg 2008
created: 2008-10-25T11:00:00Z
modified: 2015-05-08T01:10:54Z
discontinued: false
dominant: false
experience: experience/None
group u list: _232_
m user id: 0
owner id: 1673
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: N-terminal fragment (Amino acids1-156) of the monomeric[5] yellow fluorescent protein variant ???Venus??? [4], designed for fusion/BiFC[1,3]. Reassembly with Part Bba_K157007 (Split-Venus-cYFP) generates the complete, working YFP ???Venus???, whereas combination with the C-terminal fragment (Part Bba_K157005) of the monomeric cyan fluorescent Protein ???CeruleanR206K??? (Part Bba_I757009) results in green fluorescence[2].See "Part Design" for references.
mutable notes: Between BioBrick 1.0 sites this part is flanked 5' by NgoMI(=NgoMIV) and 3' AgeI(=PinAI) to facilitate in frame cloning for protein fusions.
mutable provenance: Gene synthesis by GeneArt, optimized for expression in homo sapiens.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: N-terminal split venus YFP
sequence: https://synbiohub.org/public/igem/BBa_K157008_sequence/1

N-terminal split venus YFP
type: SequenceAnnotation
persistent identity: annotation2064750
display id: annotation2064750
version: 1
top level: BBa_K157008
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157008/annotation2064750/range2064750/1

https://synbiohub.org/public/igem/BBa_K157008/annotation2064750/range2064750/1
type: Range
persistent identity: range2064750
display id: range2064750
version: 1
direction: direction/forward
top level: BBa_K157008
start: 1
end: 468
orientation: inline

SP-Lipocal
type: ComponentDefinition
persistent identity: BBa K157038
display id: BBa_K157038
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: SP-Lipocalin-GGGSlinker-Tm-Splitluciferase2/2
creator: iGEM Team Freiburg 2008
created: 2008-10-25T11:00:00Z
modified: 2015-05-08T01:10:55Z
discontinued: false
dominant: false
experience: experience/None
group u list: _232_
m user id: 0
owner id: 1673
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: A composite part consisting of the signal peptide of Erbb1/EGF-receptor, scFV-antiNIP, GGGSflexlinker (extracellular part), the trans-membrane region of the Erbb1/EGF-receptor and the C-terminal part of the split fluorophor YFP with a fluolinker joined to its N-Terminus (intracellular part).
mutable notes: none
mutable provenance: Derived by cloning of the parts BBa_K157001, BBa_K157004, BBa_K157013, BBa_K157002 and first half of split Luciferase For further information see the description of the parts mentioned above.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: Splitluciferase2/2
sequence annotation: AgeI
sequence annotation: GGGS-Linker
sequence annotation: Scar (Thr, Gly)
sequence annotation: NgoMI
sequence annotation: SP-Lipocalin-GGGSlinker-Tm-Splitluciferase2/2
sequence annotation: SP
sequence annotation: Tm
sequence annotation: Lipocalin
sequence: https://synbiohub.org/public/igem/BBa_K157038_sequence/1

Splitluciferase2/2
type: SequenceAnnotation
persistent identity: annotation1993426
display id: annotation1993426
version: 1
top level: SP-Lipocal
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157038/annotation1993426/range1993426/1

https://synbiohub.org/public/igem/BBa_K157038/annotation1993426/range1993426/1
type: Range
persistent identity: range1993426
display id: range1993426
version: 1
direction: direction/forward
top level: SP-Lipocal
start: 742
end: 1017
orientation: inline

AgeI
type: SequenceAnnotation
persistent identity: annotation1990301
display id: annotation1990301
version: 1
top level: SP-Lipocal
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157038/annotation1990301/range1990301/1

https://synbiohub.org/public/igem/BBa_K157038/annotation1990301/range1990301/1
type: Range
persistent identity: range1990301
display id: range1990301
version: 1
direction: direction/forward
top level: SP-Lipocal
start: 1018
end: 1023
orientation: inline

GGGS-Linker
type: SequenceAnnotation
persistent identity: annotation1993422
display id: annotation1993422
version: 1
top level: SP-Lipocal
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157038/annotation1993422/range1993422/1

https://synbiohub.org/public/igem/BBa_K157038/annotation1993422/range1993422/1
type: Range
persistent identity: range1993422
display id: range1993422
version: 1
direction: direction/forward
top level: SP-Lipocal
start: 616
end: 660
orientation: inline

Scar (Thr, Gly)
type: SequenceAnnotation
persistent identity: annotation1993421
display id: annotation1993421
version: 1
top level: SP-Lipocal
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157038/annotation1993421/range1993421/1

https://synbiohub.org/public/igem/BBa_K157038/annotation1993421/range1993421/1
type: Range
persistent identity: range1993421
display id: range1993421
version: 1
direction: direction/forward
top level: SP-Lipocal
start: 610
end: 615
orientation: inline

NgoMI
type: SequenceAnnotation
persistent identity: annotation1990300
display id: annotation1990300
version: 1
top level: SP-Lipocal
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157038/annotation1990300/range1990300/1

https://synbiohub.org/public/igem/BBa_K157038/annotation1990300/range1990300/1
type: Range
persistent identity: range1990300
display id: range1990300
version: 1
direction: direction/forward
top level: SP-Lipocal
start: 4
end: 9
orientation: inline

SP-Lipocalin-GGGSlinker-Tm-Splitluciferase2/2
type: SequenceAnnotation
persistent identity: annotation1990302
display id: annotation1990302
version: 1
top level: SP-Lipocal
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K157038/annotation1990302/range1990302/1

https://synbiohub.org/public/igem/BBa_K157038/annotation1990302/range1990302/1
type: Range
persistent identity: range1990302
display id: range1990302
version: 1
direction: direction/forward
top level: SP-Lipocal
start: 1
end: 1023
orientation: inline

Scar (Thr, Gly)
type: SequenceAnnotation
persistent identity: annotation1993423
display id: annotation1993423
version: 1
top level: SP-Lipocal
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157038/annotation1993423/range1993423/1

https://synbiohub.org/public/igem/BBa_K157038/annotation1993423/range1993423/1
type: Range
persistent identity: range1993423
display id: range1993423
version: 1
direction: direction/forward
top level: SP-Lipocal
start: 661
end: 666
orientation: inline

SP
type: SequenceAnnotation
persistent identity: annotation1993418
display id: annotation1993418
version: 1
top level: SP-Lipocal
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157038/annotation1993418/range1993418/1

https://synbiohub.org/public/igem/BBa_K157038/annotation1993418/range1993418/1
type: Range
persistent identity: range1993418
display id: range1993418
version: 1
direction: direction/forward
top level: SP-Lipocal
start: 10
end: 81
orientation: inline

Scar (Thr, Gly)
type: SequenceAnnotation
persistent identity: annotation1993419
display id: annotation1993419
version: 1
top level: SP-Lipocal
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157038/annotation1993419/range1993419/1

https://synbiohub.org/public/igem/BBa_K157038/annotation1993419/range1993419/1
type: Range
persistent identity: range1993419
display id: range1993419
version: 1
direction: direction/forward
top level: SP-Lipocal
start: 82
end: 87
orientation: inline

Tm
type: SequenceAnnotation
persistent identity: annotation1993424
display id: annotation1993424
version: 1
top level: SP-Lipocal
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157038/annotation1993424/range1993424/1

https://synbiohub.org/public/igem/BBa_K157038/annotation1993424/range1993424/1
type: Range
persistent identity: range1993424
display id: range1993424
version: 1
direction: direction/forward
top level: SP-Lipocal
start: 667
end: 735
orientation: inline

Scar (Thr, Gly)
type: SequenceAnnotation
persistent identity: annotation1993425
display id: annotation1993425
version: 1
top level: SP-Lipocal
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157038/annotation1993425/range1993425/1

https://synbiohub.org/public/igem/BBa_K157038/annotation1993425/range1993425/1
type: Range
persistent identity: range1993425
display id: range1993425
version: 1
direction: direction/forward
top level: SP-Lipocal
start: 736
end: 741
orientation: inline

Lipocalin
type: SequenceAnnotation
persistent identity: annotation1993420
display id: annotation1993420
version: 1
top level: SP-Lipocal
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157038/annotation1993420/range1993420/1

https://synbiohub.org/public/igem/BBa_K157038/annotation1993420/range1993420/1
type: Range
persistent identity: range1993420
display id: range1993420
version: 1
direction: direction/forward
top level: SP-Lipocal
start: 88
end: 609
orientation: inline

Red
type: ComponentDefinition
persistent identity: BBa K525499
display id: BBa_K525499
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: NADP+ Oxidoreductase from Escherichia coli
creator: Anna Drong
created: 2011-09-09T11:00:00Z
modified: 2015-05-08T01:12:34Z
discontinued: false
dominant: false
experience: experience/None
group u list: _690_
m user id: 0
owner id: 8543
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: NADP+ Oxidoreductase
mutable notes: PCR from E.coli
mutable provenance: Escherichia coli
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: Reductase
sequence annotation: AgeI
sequence annotation: TAA
sequence annotation: NgoMIV
sequence annotation: ATG
sequence: https://synbiohub.org/public/igem/BBa_K525499_sequence/1

Reductase
type: SequenceAnnotation
persistent identity: annotation2130005
display id: annotation2130005
version: 1
top level: Red
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K525499/annotation2130005/range2130005/1

https://synbiohub.org/public/igem/BBa_K525499/annotation2130005/range2130005/1
type: Range
persistent identity: range2130005
display id: range2130005
version: 1
direction: direction/forward
top level: Red
start: 1
end: 759
orientation: inline

AgeI
type: SequenceAnnotation
persistent identity: annotation2130008
display id: annotation2130008
version: 1
top level: Red
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K525499/annotation2130008/range2130008/1

https://synbiohub.org/public/igem/BBa_K525499/annotation2130008/range2130008/1
type: Range
persistent identity: range2130008
display id: range2130008
version: 1
direction: direction/forward
top level: Red
start: 751
end: 756
orientation: inline

TAA
type: SequenceAnnotation
persistent identity: annotation2130009
display id: annotation2130009
version: 1
top level: Red
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K525499/annotation2130009/range2130009/1

https://synbiohub.org/public/igem/BBa_K525499/annotation2130009/range2130009/1
type: Range
persistent identity: range2130009
display id: range2130009
version: 1
direction: direction/forward
top level: Red
start: 757
end: 759
orientation: inline

NgoMIV
type: SequenceAnnotation
persistent identity: annotation2130007
display id: annotation2130007
version: 1
top level: Red
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K525499/annotation2130007/range2130007/1

https://synbiohub.org/public/igem/BBa_K525499/annotation2130007/range2130007/1
type: Range
persistent identity: range2130007
display id: range2130007
version: 1
direction: direction/forward
top level: Red
start: 4
end: 9
orientation: inline

ATG
type: SequenceAnnotation
persistent identity: annotation2130006
display id: annotation2130006
version: 1
top level: Red
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K525499/annotation2130006/range2130006/1

https://synbiohub.org/public/igem/BBa_K525499/annotation2130006/range2130006/1
type: Range
persistent identity: range2130006
display id: range2130006
version: 1
direction: direction/forward
top level: Red
start: 1
end: 3
orientation: inline

BBa_K525001
type: ComponentDefinition
persistent identity: BBa K525001
display id: BBa_K525001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Fusion protein of SgsE, NADP+ Oxidoreductase, BisdA and BisdB with PT7/lac
creator: Anna Drong
created: 2011-09-10T11:00:00Z
modified: 2015-05-08T01:12:34Z
discontinued: false
dominant: false
experience: experience/None
group u list: _690_
m user id: 0
owner id: 8543
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: PT7 + RBS + SgsE || Red || BisdA || BisdB ; RCU 25
mutable notes: Assembled RCU 25
mutable provenance: BBa_K525303, BBa_K525499, BBa_K123000, BBa_K123001
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: Reductase
sequence annotation: BisdB
sequence annotation: ATG
sequence annotation: AgeI
sequence annotation: TAA
sequence annotation: K525301
sequence annotation: RBS
sequence annotation: SgsE domain
sequence annotation: lac operator
sequence annotation: T7 promoter
sequence annotation: BisdA
sequence annotation: Freiburg Scar
sequence annotation: B0034
sequence annotation: K525560
sequence: https://synbiohub.org/public/igem/BBa_K525001_sequence/1

Reductase
type: SequenceAnnotation
persistent identity: annotation2161003
display id: annotation2161003
version: 1
top level: BBa_K525001
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K525001/annotation2161003/range2161003/1

https://synbiohub.org/public/igem/BBa_K525001/annotation2161003/range2161003/1
type: Range
persistent identity: range2161003
display id: range2161003
version: 1
direction: direction/forward
top level: BBa_K525001
start: 2407
end: 3147
orientation: inline

BisdB
type: SequenceAnnotation
persistent identity: annotation2160992
display id: annotation2160992
version: 1
top level: BBa_K525001
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K525001/annotation2160992/range2160992/1

https://synbiohub.org/public/igem/BBa_K525001/annotation2160992/range2160992/1
type: Range
persistent identity: range2160992
display id: range2160992
version: 1
direction: direction/forward
top level: BBa_K525001
start: 3472
end: 4746
orientation: inline

ATG
type: SequenceAnnotation
persistent identity: annotation2160961
display id: annotation2160961
version: 1
top level: BBa_K525001
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K525001/annotation2160961/range2160961/1

https://synbiohub.org/public/igem/BBa_K525001/annotation2160961/range2160961/1
type: Range
persistent identity: range2160961
display id: range2160961
version: 1
direction: direction/forward
top level: BBa_K525001
start: 73
end: 75
orientation: inline

AgeI
type: SequenceAnnotation
persistent identity: annotation2160991
display id: annotation2160991
version: 1
top level: BBa_K525001
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K525001/annotation2160991/range2160991/1

https://synbiohub.org/public/igem/BBa_K525001/annotation2160991/range2160991/1
type: Range
persistent identity: range2160991
display id: range2160991
version: 1
direction: direction/forward
top level: BBa_K525001
start: 4738
end: 4743
orientation: inline

TAA
type: SequenceAnnotation
persistent identity: annotation2160990
display id: annotation2160990
version: 1
top level: BBa_K525001
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K525001/annotation2160990/range2160990/1

https://synbiohub.org/public/igem/BBa_K525001/annotation2160990/range2160990/1
type: Range
persistent identity: range2160990
display id: range2160990
version: 1
direction: direction/forward
top level: BBa_K525001
start: 4744
end: 4746
orientation: inline

K525301
type: SequenceAnnotation
persistent identity: annotation2160962
display id: annotation2160962
version: 1
top level: BBa_K525001
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K525001/annotation2160962/range2160962/1

https://synbiohub.org/public/igem/BBa_K525001/annotation2160962/range2160962/1
type: Range
persistent identity: range2160962
display id: range2160962
version: 1
direction: direction/forward
top level: BBa_K525001
start: 73
end: 2400
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2160960
display id: annotation2160960
version: 1
top level: BBa_K525001
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K525001/annotation2160960/range2160960/1

https://synbiohub.org/public/igem/BBa_K525001/annotation2160960/range2160960/1
type: Range
persistent identity: range2160960
display id: range2160960
version: 1
direction: direction/forward
top level: BBa_K525001
start: 56
end: 59
orientation: inline

SgsE domain
type: SequenceAnnotation
persistent identity: annotation2161004
display id: annotation2161004
version: 1
top level: BBa_K525001
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K525001/annotation2161004/range2161004/1

https://synbiohub.org/public/igem/BBa_K525001/annotation2161004/range2161004/1
type: Range
persistent identity: range2161004
display id: range2161004
version: 1
direction: direction/forward
top level: BBa_K525001
start: 76
end: 2400
orientation: inline

lac operator
type: SequenceAnnotation
persistent identity: annotation2160958
display id: annotation2160958
version: 1
top level: BBa_K525001
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K525001/annotation2160958/range2160958/1

https://synbiohub.org/public/igem/BBa_K525001/annotation2160958/range2160958/1
type: Range
persistent identity: range2160958
display id: range2160958
version: 1
direction: direction/forward
top level: BBa_K525001
start: 20
end: 44
orientation: inline

T7 promoter
type: SequenceAnnotation
persistent identity: annotation2160957
display id: annotation2160957
version: 1
top level: BBa_K525001
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K525001/annotation2160957/range2160957/1

https://synbiohub.org/public/igem/BBa_K525001/annotation2160957/range2160957/1
type: Range
persistent identity: range2160957
display id: range2160957
version: 1
direction: direction/forward
top level: BBa_K525001
start: 1
end: 20
orientation: inline

BisdA
type: SequenceAnnotation
persistent identity: annotation2161002
display id: annotation2161002
version: 1
top level: BBa_K525001
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K525001/annotation2161002/range2161002/1

https://synbiohub.org/public/igem/BBa_K525001/annotation2161002/range2161002/1
type: Range
persistent identity: range2161002
display id: range2161002
version: 1
direction: direction/forward
top level: BBa_K525001
start: 3154
end: 3465
orientation: inline

Freiburg Scar
type: SequenceAnnotation
persistent identity: annotation2160963
display id: annotation2160963
version: 1
top level: BBa_K525001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K525001/annotation2160963/range2160963/1

https://synbiohub.org/public/igem/BBa_K525001/annotation2160963/range2160963/1
type: Range
persistent identity: range2160963
display id: range2160963
version: 1
direction: direction/forward
top level: BBa_K525001
start: 2401
end: 2406
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation2160959
display id: annotation2160959
version: 1
top level: BBa_K525001
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K525001/annotation2160959/range2160959/1

https://synbiohub.org/public/igem/BBa_K525001/annotation2160959/range2160959/1
type: Range
persistent identity: range2160959
display id: range2160959
version: 1
direction: direction/forward
top level: BBa_K525001
start: 53
end: 64
orientation: inline

Freiburg Scar
type: SequenceAnnotation
persistent identity: annotation2160965
display id: annotation2160965
version: 1
top level: BBa_K525001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K525001/annotation2160965/range2160965/1

https://synbiohub.org/public/igem/BBa_K525001/annotation2160965/range2160965/1
type: Range
persistent identity: range2160965
display id: range2160965
version: 1
direction: direction/forward
top level: BBa_K525001
start: 3148
end: 3153
orientation: inline

Freiburg Scar
type: SequenceAnnotation
persistent identity: annotation2160989
display id: annotation2160989
version: 1
top level: BBa_K525001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K525001/annotation2160989/range2160989/1

https://synbiohub.org/public/igem/BBa_K525001/annotation2160989/range2160989/1
type: Range
persistent identity: range2160989
display id: range2160989
version: 1
direction: direction/forward
top level: BBa_K525001
start: 3466
end: 3471
orientation: inline

K525560
type: SequenceAnnotation
persistent identity: annotation2160964
display id: annotation2160964
version: 1
top level: BBa_K525001
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K525001/annotation2160964/range2160964/1

https://synbiohub.org/public/igem/BBa_K525001/annotation2160964/range2160964/1
type: Range
persistent identity: range2160964
display id: range2160964
version: 1
direction: direction/forward
top level: BBa_K525001
start: 2407
end: 4746
orientation: inline

SP-AntiNIP
type: ComponentDefinition
persistent identity: BBa K157029
display id: BBa_K157029
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: SP-AntiNIP-GGGSlinker-Tm-nCFP
creator: iGEM Team Freiburg 2008
created: 2008-10-25T11:00:00Z
modified: 2015-05-08T01:10:55Z
discontinued: false
dominant: false
experience: experience/None
group u list: _232_
m user id: 0
owner id: 1673
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Part consists of the signal peptide of Erbb1/EGF-receptor, scFV-antiNIP, GGGSflexlinker (extracellular part), the trans-membrane region of the Erbb1/EGF-receptor and the C-terminal part of the split fluorophor YFP with a fluolinker joined to its N-Terminus (intracellular part).
mutable notes: none
mutable provenance: Derived by cloning of the parts BBa_K157001, BBa_K157003, BBa_K157013, BBa_K157002, BBa_K157006. For further information see the description of the parts mentioned above.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: Tm
sequence annotation: Scar (Thr, Gly)
sequence annotation: SP
sequence annotation: N-Term_CFP
sequence annotation: GGGS-Linker
sequence annotation: SP-AntiNIP-GGGSlinker-Tm-nCFP
sequence annotation: Sc-Fv_anti-NIP
sequence annotation: AgeI
sequence annotation: NgoMI
sequence: https://synbiohub.org/public/igem/BBa_K157029_sequence/1

Tm
type: SequenceAnnotation
persistent identity: annotation1993306
display id: annotation1993306
version: 1
top level: SP-AntiNIP
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157029/annotation1993306/range1993306/1

https://synbiohub.org/public/igem/BBa_K157029/annotation1993306/range1993306/1
type: Range
persistent identity: range1993306
display id: range1993306
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 880
end: 948
orientation: inline

Scar (Thr, Gly)
type: SequenceAnnotation
persistent identity: annotation1993301
display id: annotation1993301
version: 1
top level: SP-AntiNIP
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157029/annotation1993301/range1993301/1

https://synbiohub.org/public/igem/BBa_K157029/annotation1993301/range1993301/1
type: Range
persistent identity: range1993301
display id: range1993301
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 82
end: 87
orientation: inline

Scar (Thr, Gly)
type: SequenceAnnotation
persistent identity: annotation1993303
display id: annotation1993303
version: 1
top level: SP-AntiNIP
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157029/annotation1993303/range1993303/1

https://synbiohub.org/public/igem/BBa_K157029/annotation1993303/range1993303/1
type: Range
persistent identity: range1993303
display id: range1993303
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 823
end: 828
orientation: inline

Scar (Thr, Gly)
type: SequenceAnnotation
persistent identity: annotation1993307
display id: annotation1993307
version: 1
top level: SP-AntiNIP
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157029/annotation1993307/range1993307/1

https://synbiohub.org/public/igem/BBa_K157029/annotation1993307/range1993307/1
type: Range
persistent identity: range1993307
display id: range1993307
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 949
end: 954
orientation: inline

SP
type: SequenceAnnotation
persistent identity: annotation1993300
display id: annotation1993300
version: 1
top level: SP-AntiNIP
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157029/annotation1993300/range1993300/1

https://synbiohub.org/public/igem/BBa_K157029/annotation1993300/range1993300/1
type: Range
persistent identity: range1993300
display id: range1993300
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 10
end: 81
orientation: inline

N-Term_CFP
type: SequenceAnnotation
persistent identity: annotation1993308
display id: annotation1993308
version: 1
top level: SP-AntiNIP
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157029/annotation1993308/range1993308/1

https://synbiohub.org/public/igem/BBa_K157029/annotation1993308/range1993308/1
type: Range
persistent identity: range1993308
display id: range1993308
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 955
end: 1419
orientation: inline

GGGS-Linker
type: SequenceAnnotation
persistent identity: annotation1993304
display id: annotation1993304
version: 1
top level: SP-AntiNIP
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157029/annotation1993304/range1993304/1

https://synbiohub.org/public/igem/BBa_K157029/annotation1993304/range1993304/1
type: Range
persistent identity: range1993304
display id: range1993304
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 829
end: 873
orientation: inline

SP-AntiNIP-GGGSlinker-Tm-nCFP
type: SequenceAnnotation
persistent identity: annotation1990253
display id: annotation1990253
version: 1
top level: SP-AntiNIP
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K157029/annotation1990253/range1990253/1

https://synbiohub.org/public/igem/BBa_K157029/annotation1990253/range1990253/1
type: Range
persistent identity: range1990253
display id: range1990253
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 1
end: 1425
orientation: inline

Sc-Fv_anti-NIP
type: SequenceAnnotation
persistent identity: annotation1993302
display id: annotation1993302
version: 1
top level: SP-AntiNIP
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157029/annotation1993302/range1993302/1

https://synbiohub.org/public/igem/BBa_K157029/annotation1993302/range1993302/1
type: Range
persistent identity: range1993302
display id: range1993302
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 88
end: 822
orientation: inline

AgeI
type: SequenceAnnotation
persistent identity: annotation1990245
display id: annotation1990245
version: 1
top level: SP-AntiNIP
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157029/annotation1990245/range1990245/1

https://synbiohub.org/public/igem/BBa_K157029/annotation1990245/range1990245/1
type: Range
persistent identity: range1990245
display id: range1990245
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 1420
end: 1425
orientation: inline

NgoMI
type: SequenceAnnotation
persistent identity: annotation1990244
display id: annotation1990244
version: 1
top level: SP-AntiNIP
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157029/annotation1990244/range1990244/1

https://synbiohub.org/public/igem/BBa_K157029/annotation1990244/range1990244/1
type: Range
persistent identity: range1990244
display id: range1990244
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 4
end: 9
orientation: inline

Scar (Thr, Gly)
type: SequenceAnnotation
persistent identity: annotation1993305
display id: annotation1993305
version: 1
top level: SP-AntiNIP
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157029/annotation1993305/range1993305/1

https://synbiohub.org/public/igem/BBa_K157029/annotation1993305/range1993305/1
type: Range
persistent identity: range1993305
display id: range1993305
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 874
end: 879
orientation: inline

Tm-Split-L
type: ComponentDefinition
persistent identity: BBa K157020
display id: BBa_K157020
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Tm-Split-Luciferase1/2
creator: iGEM Team Freiburg 2008
created: 2008-10-25T11:00:00Z
modified: 2015-05-08T01:10:54Z
discontinued: false
dominant: false
experience: experience/None
group u list: _232_
m user id: 0
owner id: 1673
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Part 1 of the Split- Luciferase fused to the trans-membrane region of the Erbb1/EGF-receptor.
mutable notes: none
mutable provenance: Derived by cloning of the parts BBa_K157002 and Split-Luciferase fragment 1. For further information see the description of the parts mentioned above.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: Split-Luciferase1
sequence annotation: NgoMI
sequence annotation: Tm-Split-Luciferase1/2
sequence annotation: Tm
sequence annotation: Scar (Thr, Gly)
sequence annotation: AgeI
sequence: https://synbiohub.org/public/igem/BBa_K157020_sequence/1

Split-Luciferase1
type: SequenceAnnotation
persistent identity: annotation1991536
display id: annotation1991536
version: 1
top level: Tm-Split-L
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157020/annotation1991536/range1991536/1

https://synbiohub.org/public/igem/BBa_K157020/annotation1991536/range1991536/1
type: Range
persistent identity: range1991536
display id: range1991536
version: 1
direction: direction/forward
top level: Tm-Split-L
start: 85
end: 312
orientation: inline

NgoMI
type: SequenceAnnotation
persistent identity: annotation1990037
display id: annotation1990037
version: 1
top level: Tm-Split-L
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157020/annotation1990037/range1990037/1

https://synbiohub.org/public/igem/BBa_K157020/annotation1990037/range1990037/1
type: Range
persistent identity: range1990037
display id: range1990037
version: 1
direction: direction/forward
top level: Tm-Split-L
start: 4
end: 9
orientation: inline

Tm-Split-Luciferase1/2
type: SequenceAnnotation
persistent identity: annotation1990063
display id: annotation1990063
version: 1
top level: Tm-Split-L
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K157020/annotation1990063/range1990063/1

https://synbiohub.org/public/igem/BBa_K157020/annotation1990063/range1990063/1
type: Range
persistent identity: range1990063
display id: range1990063
version: 1
direction: direction/forward
top level: Tm-Split-L
start: 1
end: 318
orientation: inline

Tm
type: SequenceAnnotation
persistent identity: annotation1991534
display id: annotation1991534
version: 1
top level: Tm-Split-L
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157020/annotation1991534/range1991534/1

https://synbiohub.org/public/igem/BBa_K157020/annotation1991534/range1991534/1
type: Range
persistent identity: range1991534
display id: range1991534
version: 1
direction: direction/forward
top level: Tm-Split-L
start: 10
end: 78
orientation: inline

Scar (Thr, Gly)
type: SequenceAnnotation
persistent identity: annotation1991535
display id: annotation1991535
version: 1
top level: Tm-Split-L
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157020/annotation1991535/range1991535/1

https://synbiohub.org/public/igem/BBa_K157020/annotation1991535/range1991535/1
type: Range
persistent identity: range1991535
display id: range1991535
version: 1
direction: direction/forward
top level: Tm-Split-L
start: 79
end: 84
orientation: inline

AgeI
type: SequenceAnnotation
persistent identity: annotation1990038
display id: annotation1990038
version: 1
top level: Tm-Split-L
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157020/annotation1990038/range1990038/1

https://synbiohub.org/public/igem/BBa_K157020/annotation1990038/range1990038/1
type: Range
persistent identity: range1990038
display id: range1990038
version: 1
direction: direction/forward
top level: Tm-Split-L
start: 313
end: 318
orientation: inline

SbpA|LigA
type: ComponentDefinition
persistent identity: BBa K525006
display id: BBa_K525006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Fusion Protein of S-Layer SbpA and LigA
creator: Manuel Wittchen
created: 2011-10-24T11:00:00Z
modified: 2015-05-08T01:12:34Z
discontinued: false
dominant: false
experience: experience/None
group u list: _690_
m user id: 0
owner id: 8718
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Fusion Protein of S-Layer SbpA and LigA
mutable notes: Assembled via Freiburg Standard Assembly (RFC 25)
mutable provenance: Assembled fusion protein. SbpA was synthesized, LigA is the natural ligase A of E. coli
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: ATG
sequence annotation: K525401
sequence annotation: SbpA domain
sequence annotation: lac operator
sequence annotation: AgeI
sequence annotation: RBS
sequence annotation: B0034
sequence annotation: LigA domain
sequence annotation: Freiburg Scar
sequence annotation: TAA
sequence annotation: T7 Promotor
sequence annotation: NgoMIV
sequence annotation: K525720
sequence: https://synbiohub.org/public/igem/BBa_K525006_sequence/1

ATG
type: SequenceAnnotation
persistent identity: annotation2160490
display id: annotation2160490
version: 1
top level: SbpA|LigA
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K525006/annotation2160490/range2160490/1

https://synbiohub.org/public/igem/BBa_K525006/annotation2160490/range2160490/1
type: Range
persistent identity: range2160490
display id: range2160490
version: 1
direction: direction/forward
top level: SbpA|LigA
start: 73
end: 75
orientation: inline

K525401
type: SequenceAnnotation
persistent identity: annotation2160485
display id: annotation2160485
version: 1
top level: SbpA|LigA
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K525006/annotation2160485/range2160485/1

https://synbiohub.org/public/igem/BBa_K525006/annotation2160485/range2160485/1
type: Range
persistent identity: range2160485
display id: range2160485
version: 1
direction: direction/forward
top level: SbpA|LigA
start: 73
end: 3193
orientation: inline

SbpA domain
type: SequenceAnnotation
persistent identity: annotation2160489
display id: annotation2160489
version: 1
top level: SbpA|LigA
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K525006/annotation2160489/range2160489/1

https://synbiohub.org/public/igem/BBa_K525006/annotation2160489/range2160489/1
type: Range
persistent identity: range2160489
display id: range2160489
version: 1
direction: direction/forward
top level: SbpA|LigA
start: 73
end: 3192
orientation: inline

lac operator
type: SequenceAnnotation
persistent identity: annotation2160487
display id: annotation2160487
version: 1
top level: SbpA|LigA
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K525006/annotation2160487/range2160487/1

https://synbiohub.org/public/igem/BBa_K525006/annotation2160487/range2160487/1
type: Range
persistent identity: range2160487
display id: range2160487
version: 1
direction: direction/forward
top level: SbpA|LigA
start: 20
end: 44
orientation: inline

AgeI
type: SequenceAnnotation
persistent identity: annotation2160495
display id: annotation2160495
version: 1
top level: SbpA|LigA
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K525006/annotation2160495/range2160495/1

https://synbiohub.org/public/igem/BBa_K525006/annotation2160495/range2160495/1
type: Range
persistent identity: range2160495
display id: range2160495
version: 1
direction: direction/forward
top level: SbpA|LigA
start: 5209
end: 5215
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2161006
display id: annotation2161006
version: 1
top level: SbpA|LigA
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K525006/annotation2161006/range2161006/1

https://synbiohub.org/public/igem/BBa_K525006/annotation2161006/range2161006/1
type: Range
persistent identity: range2161006
display id: range2161006
version: 1
direction: direction/forward
top level: SbpA|LigA
start: 56
end: 59
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation2160488
display id: annotation2160488
version: 1
top level: SbpA|LigA
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K525006/annotation2160488/range2160488/1

https://synbiohub.org/public/igem/BBa_K525006/annotation2160488/range2160488/1
type: Range
persistent identity: range2160488
display id: range2160488
version: 1
direction: direction/forward
top level: SbpA|LigA
start: 53
end: 64
orientation: inline

LigA domain
type: SequenceAnnotation
persistent identity: annotation2160493
display id: annotation2160493
version: 1
top level: SbpA|LigA
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K525006/annotation2160493/range2160493/1

https://synbiohub.org/public/igem/BBa_K525006/annotation2160493/range2160493/1
type: Range
persistent identity: range2160493
display id: range2160493
version: 1
direction: direction/forward
top level: SbpA|LigA
start: 3199
end: 5208
orientation: inline

Freiburg Scar
type: SequenceAnnotation
persistent identity: annotation2160491
display id: annotation2160491
version: 1
top level: SbpA|LigA
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K525006/annotation2160491/range2160491/1

https://synbiohub.org/public/igem/BBa_K525006/annotation2160491/range2160491/1
type: Range
persistent identity: range2160491
display id: range2160491
version: 1
direction: direction/forward
top level: SbpA|LigA
start: 3193
end: 3198
orientation: inline

TAA
type: SequenceAnnotation
persistent identity: annotation2160484
display id: annotation2160484
version: 1
top level: SbpA|LigA
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K525006/annotation2160484/range2160484/1

https://synbiohub.org/public/igem/BBa_K525006/annotation2160484/range2160484/1
type: Range
persistent identity: range2160484
display id: range2160484
version: 1
direction: direction/forward
top level: SbpA|LigA
start: 5214
end: 5217
orientation: inline

T7 Promotor
type: SequenceAnnotation
persistent identity: annotation2160486
display id: annotation2160486
version: 1
top level: SbpA|LigA
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K525006/annotation2160486/range2160486/1

https://synbiohub.org/public/igem/BBa_K525006/annotation2160486/range2160486/1
type: Range
persistent identity: range2160486
display id: range2160486
version: 1
direction: direction/forward
top level: SbpA|LigA
start: 1
end: 20
orientation: inline

NgoMIV
type: SequenceAnnotation
persistent identity: annotation2160494
display id: annotation2160494
version: 1
top level: SbpA|LigA
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K525006/annotation2160494/range2160494/1

https://synbiohub.org/public/igem/BBa_K525006/annotation2160494/range2160494/1
type: Range
persistent identity: range2160494
display id: range2160494
version: 1
direction: direction/forward
top level: SbpA|LigA
start: 76
end: 82
orientation: inline

K525720
type: SequenceAnnotation
persistent identity: annotation2160492
display id: annotation2160492
version: 1
top level: SbpA|LigA
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K525006/annotation2160492/range2160492/1

https://synbiohub.org/public/igem/BBa_K525006/annotation2160492/range2160492/1
type: Range
persistent identity: range2160492
display id: range2160492
version: 1
direction: direction/forward
top level: SbpA|LigA
start: 3194
end: 5217
orientation: inline

SP-AntiNIP
type: ComponentDefinition
persistent identity: BBa K157028
display id: BBa_K157028
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: SP-AntiNIP-GGGSlinker-Tm-Fluolinker-cCFP
creator: iGEM Team Freiburg 2008
created: 2008-10-25T11:00:00Z
modified: 2015-05-08T01:10:55Z
discontinued: false
dominant: false
experience: experience/None
group u list: _232_
m user id: 0
owner id: 1673
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Part consists of the signal peptide of Erbb1/EGF-receptor, scFV-antiNIP, GGGSflexlinker (extracellular part), the trans-membrane region of the Erbb1/EGF-receptor and the C-terminal part of the split fluorophor YFP with a fluolinker joined to its N-Terminus (intracellular part).
mutable notes: none
mutable provenance: Derived by cloning of the parts BBa_K157001, BBa_K157003, BBa_K157013, BBa_K157002, BBa_K157009 and BBa_K157005. For further information see the description of the parts mentioned above.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: SP
sequence annotation: NgoMI
sequence annotation: Scar (Thr, Gly)
sequence annotation: GGGS-Linker
sequence annotation: SP-AntiNIP-GGGSlinker-Tm-Fluolinker-cCFP
sequence annotation: AgeI
sequence annotation: Sc-Fv_anti-NIP
sequence annotation: Tm
sequence annotation: C-Term_CFP
sequence annotation: Fluolinker
sequence: https://synbiohub.org/public/igem/BBa_K157028_sequence/1

SP
type: SequenceAnnotation
persistent identity: annotation1993289
display id: annotation1993289
version: 1
top level: SP-AntiNIP
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157028/annotation1993289/range1993289/1

https://synbiohub.org/public/igem/BBa_K157028/annotation1993289/range1993289/1
type: Range
persistent identity: range1993289
display id: range1993289
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 10
end: 81
orientation: inline

NgoMI
type: SequenceAnnotation
persistent identity: annotation1990180
display id: annotation1990180
version: 1
top level: SP-AntiNIP
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157028/annotation1990180/range1990180/1

https://synbiohub.org/public/igem/BBa_K157028/annotation1990180/range1990180/1
type: Range
persistent identity: range1990180
display id: range1990180
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 4
end: 9
orientation: inline

Scar (Thr, Gly)
type: SequenceAnnotation
persistent identity: annotation1993296
display id: annotation1993296
version: 1
top level: SP-AntiNIP
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157028/annotation1993296/range1993296/1

https://synbiohub.org/public/igem/BBa_K157028/annotation1993296/range1993296/1
type: Range
persistent identity: range1993296
display id: range1993296
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 949
end: 954
orientation: inline

GGGS-Linker
type: SequenceAnnotation
persistent identity: annotation1993293
display id: annotation1993293
version: 1
top level: SP-AntiNIP
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157028/annotation1993293/range1993293/1

https://synbiohub.org/public/igem/BBa_K157028/annotation1993293/range1993293/1
type: Range
persistent identity: range1993293
display id: range1993293
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 829
end: 873
orientation: inline

SP-AntiNIP-GGGSlinker-Tm-Fluolinker-cCFP
type: SequenceAnnotation
persistent identity: annotation1990209
display id: annotation1990209
version: 1
top level: SP-AntiNIP
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K157028/annotation1990209/range1990209/1

https://synbiohub.org/public/igem/BBa_K157028/annotation1990209/range1990209/1
type: Range
persistent identity: range1990209
display id: range1990209
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 1
end: 1260
orientation: inline

Scar (Thr, Gly)
type: SequenceAnnotation
persistent identity: annotation1993290
display id: annotation1993290
version: 1
top level: SP-AntiNIP
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157028/annotation1993290/range1993290/1

https://synbiohub.org/public/igem/BBa_K157028/annotation1993290/range1993290/1
type: Range
persistent identity: range1993290
display id: range1993290
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 82
end: 87
orientation: inline

AgeI
type: SequenceAnnotation
persistent identity: annotation1990181
display id: annotation1990181
version: 1
top level: SP-AntiNIP
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157028/annotation1990181/range1990181/1

https://synbiohub.org/public/igem/BBa_K157028/annotation1990181/range1990181/1
type: Range
persistent identity: range1990181
display id: range1990181
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 1255
end: 1260
orientation: inline

Sc-Fv_anti-NIP
type: SequenceAnnotation
persistent identity: annotation1993291
display id: annotation1993291
version: 1
top level: SP-AntiNIP
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157028/annotation1993291/range1993291/1

https://synbiohub.org/public/igem/BBa_K157028/annotation1993291/range1993291/1
type: Range
persistent identity: range1993291
display id: range1993291
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 88
end: 822
orientation: inline

Scar (Thr, Gly)
type: SequenceAnnotation
persistent identity: annotation1993292
display id: annotation1993292
version: 1
top level: SP-AntiNIP
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157028/annotation1993292/range1993292/1

https://synbiohub.org/public/igem/BBa_K157028/annotation1993292/range1993292/1
type: Range
persistent identity: range1993292
display id: range1993292
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 823
end: 828
orientation: inline

Scar (Thr, Gly)
type: SequenceAnnotation
persistent identity: annotation1993298
display id: annotation1993298
version: 1
top level: SP-AntiNIP
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157028/annotation1993298/range1993298/1

https://synbiohub.org/public/igem/BBa_K157028/annotation1993298/range1993298/1
type: Range
persistent identity: range1993298
display id: range1993298
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 1006
end: 1011
orientation: inline

Tm
type: SequenceAnnotation
persistent identity: annotation1993295
display id: annotation1993295
version: 1
top level: SP-AntiNIP
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157028/annotation1993295/range1993295/1

https://synbiohub.org/public/igem/BBa_K157028/annotation1993295/range1993295/1
type: Range
persistent identity: range1993295
display id: range1993295
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 880
end: 948
orientation: inline

C-Term_CFP
type: SequenceAnnotation
persistent identity: annotation1993299
display id: annotation1993299
version: 1
top level: SP-AntiNIP
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157028/annotation1993299/range1993299/1

https://synbiohub.org/public/igem/BBa_K157028/annotation1993299/range1993299/1
type: Range
persistent identity: range1993299
display id: range1993299
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 1012
end: 1254
orientation: inline

Scar (Thr, Gly)
type: SequenceAnnotation
persistent identity: annotation1993294
display id: annotation1993294
version: 1
top level: SP-AntiNIP
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157028/annotation1993294/range1993294/1

https://synbiohub.org/public/igem/BBa_K157028/annotation1993294/range1993294/1
type: Range
persistent identity: range1993294
display id: range1993294
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 874
end: 879
orientation: inline

Fluolinker
type: SequenceAnnotation
persistent identity: annotation1993297
display id: annotation1993297
version: 1
top level: SP-AntiNIP
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157028/annotation1993297/range1993297/1

https://synbiohub.org/public/igem/BBa_K157028/annotation1993297/range1993297/1
type: Range
persistent identity: range1993297
display id: range1993297
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 955
end: 1005
orientation: inline

SP-AntiNIP
type: ComponentDefinition
persistent identity: BBa K157026
display id: BBa_K157026
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: SP-AntiNIP-GGGSlinker-Tm-Fluolinker-cYFP
creator: iGEM Team Freiburg 2008
created: 2008-10-25T11:00:00Z
modified: 2015-05-08T01:10:55Z
discontinued: false
dominant: false
experience: experience/None
group u list: _232_
m user id: 0
owner id: 1673
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: A composite part consisting of the signal peptide of Erbb1/EGF-receptor, scFV-antiNIP, GGGSflexlinker (extracellular part), the trans-membrane region of the Erbb1/EGF-receptor and the C-terminal part of the split fluorophor YFP with a fluolinker joined to its N-Terminus (intracellular part).
mutable notes: none
mutable provenance: Derived by cloning of the parts BBa_K157001, BBa_K157003, BBa_K157013, BBa_K157002, BBa_K157009 and BBa_K157007. For further information see the description of the parts mentioned above.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: SP
sequence annotation: Scar (Thr, Gly)
sequence annotation: Tm
sequence annotation: SP-AntiNIP-GGGSlinker-Tm-Fluolinker-cYFP
sequence annotation: C-Term_YFP
sequence annotation: GGGS-Linker
sequence annotation: Sc-Fv_anti-NIP
sequence annotation: NgoMI
sequence annotation: AgeI
sequence annotation: Fluolinker
sequence: https://synbiohub.org/public/igem/BBa_K157026_sequence/1

SP
type: SequenceAnnotation
persistent identity: annotation1992638
display id: annotation1992638
version: 1
top level: SP-AntiNIP
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157026/annotation1992638/range1992638/1

https://synbiohub.org/public/igem/BBa_K157026/annotation1992638/range1992638/1
type: Range
persistent identity: range1992638
display id: range1992638
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 10
end: 81
orientation: inline

Scar (Thr, Gly)
type: SequenceAnnotation
persistent identity: annotation1992647
display id: annotation1992647
version: 1
top level: SP-AntiNIP
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157026/annotation1992647/range1992647/1

https://synbiohub.org/public/igem/BBa_K157026/annotation1992647/range1992647/1
type: Range
persistent identity: range1992647
display id: range1992647
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 1006
end: 1011
orientation: inline

Tm
type: SequenceAnnotation
persistent identity: annotation1992644
display id: annotation1992644
version: 1
top level: SP-AntiNIP
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157026/annotation1992644/range1992644/1

https://synbiohub.org/public/igem/BBa_K157026/annotation1992644/range1992644/1
type: Range
persistent identity: range1992644
display id: range1992644
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 880
end: 948
orientation: inline

SP-AntiNIP-GGGSlinker-Tm-Fluolinker-cYFP
type: SequenceAnnotation
persistent identity: annotation1990176
display id: annotation1990176
version: 1
top level: SP-AntiNIP
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K157026/annotation1990176/range1990176/1

https://synbiohub.org/public/igem/BBa_K157026/annotation1990176/range1990176/1
type: Range
persistent identity: range1990176
display id: range1990176
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 1
end: 1260
orientation: inline

Scar (Thr, Gly)
type: SequenceAnnotation
persistent identity: annotation1992643
display id: annotation1992643
version: 1
top level: SP-AntiNIP
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157026/annotation1992643/range1992643/1

https://synbiohub.org/public/igem/BBa_K157026/annotation1992643/range1992643/1
type: Range
persistent identity: range1992643
display id: range1992643
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 874
end: 879
orientation: inline

Scar (Thr, Gly)
type: SequenceAnnotation
persistent identity: annotation1992645
display id: annotation1992645
version: 1
top level: SP-AntiNIP
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157026/annotation1992645/range1992645/1

https://synbiohub.org/public/igem/BBa_K157026/annotation1992645/range1992645/1
type: Range
persistent identity: range1992645
display id: range1992645
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 949
end: 954
orientation: inline

C-Term_YFP
type: SequenceAnnotation
persistent identity: annotation1992648
display id: annotation1992648
version: 1
top level: SP-AntiNIP
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157026/annotation1992648/range1992648/1

https://synbiohub.org/public/igem/BBa_K157026/annotation1992648/range1992648/1
type: Range
persistent identity: range1992648
display id: range1992648
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 1012
end: 1254
orientation: inline

GGGS-Linker
type: SequenceAnnotation
persistent identity: annotation1992642
display id: annotation1992642
version: 1
top level: SP-AntiNIP
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157026/annotation1992642/range1992642/1

https://synbiohub.org/public/igem/BBa_K157026/annotation1992642/range1992642/1
type: Range
persistent identity: range1992642
display id: range1992642
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 829
end: 873
orientation: inline

Scar (Thr, Gly)
type: SequenceAnnotation
persistent identity: annotation1992641
display id: annotation1992641
version: 1
top level: SP-AntiNIP
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157026/annotation1992641/range1992641/1

https://synbiohub.org/public/igem/BBa_K157026/annotation1992641/range1992641/1
type: Range
persistent identity: range1992641
display id: range1992641
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 823
end: 828
orientation: inline

Sc-Fv_anti-NIP
type: SequenceAnnotation
persistent identity: annotation1992640
display id: annotation1992640
version: 1
top level: SP-AntiNIP
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157026/annotation1992640/range1992640/1

https://synbiohub.org/public/igem/BBa_K157026/annotation1992640/range1992640/1
type: Range
persistent identity: range1992640
display id: range1992640
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 88
end: 822
orientation: inline

Scar (Thr, Gly)
type: SequenceAnnotation
persistent identity: annotation1992639
display id: annotation1992639
version: 1
top level: SP-AntiNIP
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157026/annotation1992639/range1992639/1

https://synbiohub.org/public/igem/BBa_K157026/annotation1992639/range1992639/1
type: Range
persistent identity: range1992639
display id: range1992639
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 82
end: 87
orientation: inline

NgoMI
type: SequenceAnnotation
persistent identity: annotation1990174
display id: annotation1990174
version: 1
top level: SP-AntiNIP
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157026/annotation1990174/range1990174/1

https://synbiohub.org/public/igem/BBa_K157026/annotation1990174/range1990174/1
type: Range
persistent identity: range1990174
display id: range1990174
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 4
end: 9
orientation: inline

AgeI
type: SequenceAnnotation
persistent identity: annotation1990175
display id: annotation1990175
version: 1
top level: SP-AntiNIP
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157026/annotation1990175/range1990175/1

https://synbiohub.org/public/igem/BBa_K157026/annotation1990175/range1990175/1
type: Range
persistent identity: range1990175
display id: range1990175
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 1255
end: 1260
orientation: inline

Fluolinker
type: SequenceAnnotation
persistent identity: annotation1992646
display id: annotation1992646
version: 1
top level: SP-AntiNIP
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157026/annotation1992646/range1992646/1

https://synbiohub.org/public/igem/BBa_K157026/annotation1992646/range1992646/1
type: Range
persistent identity: range1992646
display id: range1992646
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 955
end: 1005
orientation: inline

BBa_K157002
type: ComponentDefinition
persistent identity: BBa K157002
display id: BBa_K157002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Transmembrane region of the EGF-Receptor (ErbB-1)
creator: iGEM Team Freiburg 2008
created: 2008-10-25T11:00:00Z
modified: 2015-05-08T01:10:54Z
discontinued: false
dominant: false
experience: experience/None
group u list: _232_
m user id: 0
owner id: 1673
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Helical, single-span transmembrane region of the human EGF-Receptor type ErbB-1, sequence taken from UniProtKB/Swiss-Prot entry P00533.
mutable notes: Between BioBrick 1.0 sites this part is flanked 5' by NgoMI(=NgoMIV) and 3' AgeI(=PinAI) to facilitate in frame cloning for protein fusions.
mutable provenance: Transmembrane region of the human EGF-Receptor type ErbB-1. Sequence taken from UniProtKB/Swiss-Prot entry P00533. Gene synthesis by ATG:biosynthetics, optimized for expression in homo sapiens by iGEM-Team Freiburg 2008.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: EGFR-Transmembrane region
sequence: https://synbiohub.org/public/igem/BBa_K157002_sequence/1

EGFR-Transmembrane region
type: SequenceAnnotation
persistent identity: annotation2064675
display id: annotation2064675
version: 1
top level: BBa_K157002
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157002/annotation2064675/range2064675/1

https://synbiohub.org/public/igem/BBa_K157002/annotation2064675/range2064675/1
type: Range
persistent identity: range2064675
display id: range2064675
version: 1
direction: direction/forward
top level: BBa_K157002
start: 1
end: 69
orientation: inline

SbpA | RFP
type: ComponentDefinition
persistent identity: BBa K525404
display id: BBa_K525404
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Fusion Protein of S-Layer SbpA and mCherry RFP
creator: Timo Wolf
created: 2011-09-09T11:00:00Z
modified: 2016-01-26T09:41:39Z
discontinued: false
dominant: false
experience: experience/None
group u list: _690_
m user id: 4206
owner id: 6668
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: Fusion Protein of S-Layer sbpA and mCherry RFP
mutable notes: Fusion Protein of BBa_K525403 and BBa_J18932
mutable provenance: Fusion Protein of S-Layer sbpA and mCherry RFP
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: SbpA domain
sequence annotation: mCherry (RFP)
sequence annotation: AgeI
sequence annotation: TAA
sequence annotation: lac operator
sequence annotation: B0034
sequence annotation: NgoMIV
sequence annotation: ATG
sequence annotation: K525401
sequence annotation: RBS
sequence annotation: Freiburg scar
sequence annotation: T7 promoter
sequence annotation: J18932
sequence: https://synbiohub.org/public/igem/BBa_K525404_sequence/1

SbpA domain
type: SequenceAnnotation
persistent identity: annotation2129913
display id: annotation2129913
version: 1
top level: SbpA | RFP
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K525404/annotation2129913/range2129913/1

https://synbiohub.org/public/igem/BBa_K525404/annotation2129913/range2129913/1
type: Range
persistent identity: range2129913
display id: range2129913
version: 1
direction: direction/forward
top level: SbpA | RFP
start: 73
end: 3192
orientation: inline

mCherry (RFP)
type: SequenceAnnotation
persistent identity: annotation2129915
display id: annotation2129915
version: 1
top level: SbpA | RFP
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K525404/annotation2129915/range2129915/1

https://synbiohub.org/public/igem/BBa_K525404/annotation2129915/range2129915/1
type: Range
persistent identity: range2129915
display id: range2129915
version: 1
direction: direction/forward
top level: SbpA | RFP
start: 3199
end: 3912
orientation: inline

AgeI
type: SequenceAnnotation
persistent identity: annotation2129910
display id: annotation2129910
version: 1
top level: SbpA | RFP
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K525404/annotation2129910/range2129910/1

https://synbiohub.org/public/igem/BBa_K525404/annotation2129910/range2129910/1
type: Range
persistent identity: range2129910
display id: range2129910
version: 1
direction: direction/forward
top level: SbpA | RFP
start: 3904
end: 3909
orientation: inline

TAA
type: SequenceAnnotation
persistent identity: annotation2129911
display id: annotation2129911
version: 1
top level: SbpA | RFP
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K525404/annotation2129911/range2129911/1

https://synbiohub.org/public/igem/BBa_K525404/annotation2129911/range2129911/1
type: Range
persistent identity: range2129911
display id: range2129911
version: 1
direction: direction/forward
top level: SbpA | RFP
start: 3910
end: 3912
orientation: inline

lac operator
type: SequenceAnnotation
persistent identity: annotation2128865
display id: annotation2128865
version: 1
top level: SbpA | RFP
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K525404/annotation2128865/range2128865/1

https://synbiohub.org/public/igem/BBa_K525404/annotation2128865/range2128865/1
type: Range
persistent identity: range2128865
display id: range2128865
version: 1
direction: direction/forward
top level: SbpA | RFP
start: 20
end: 44
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation2129905
display id: annotation2129905
version: 1
top level: SbpA | RFP
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K525404/annotation2129905/range2129905/1

https://synbiohub.org/public/igem/BBa_K525404/annotation2129905/range2129905/1
type: Range
persistent identity: range2129905
display id: range2129905
version: 1
direction: direction/forward
top level: SbpA | RFP
start: 53
end: 64
orientation: inline

NgoMIV
type: SequenceAnnotation
persistent identity: annotation2129909
display id: annotation2129909
version: 1
top level: SbpA | RFP
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K525404/annotation2129909/range2129909/1

https://synbiohub.org/public/igem/BBa_K525404/annotation2129909/range2129909/1
type: Range
persistent identity: range2129909
display id: range2129909
version: 1
direction: direction/forward
top level: SbpA | RFP
start: 76
end: 81
orientation: inline

ATG
type: SequenceAnnotation
persistent identity: annotation2129907
display id: annotation2129907
version: 1
top level: SbpA | RFP
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K525404/annotation2129907/range2129907/1

https://synbiohub.org/public/igem/BBa_K525404/annotation2129907/range2129907/1
type: Range
persistent identity: range2129907
display id: range2129907
version: 1
direction: direction/forward
top level: SbpA | RFP
start: 73
end: 75
orientation: inline

K525401
type: SequenceAnnotation
persistent identity: annotation2129908
display id: annotation2129908
version: 1
top level: SbpA | RFP
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K525404/annotation2129908/range2129908/1

https://synbiohub.org/public/igem/BBa_K525404/annotation2129908/range2129908/1
type: Range
persistent identity: range2129908
display id: range2129908
version: 1
direction: direction/forward
top level: SbpA | RFP
start: 73
end: 3192
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2129906
display id: annotation2129906
version: 1
top level: SbpA | RFP
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K525404/annotation2129906/range2129906/1

https://synbiohub.org/public/igem/BBa_K525404/annotation2129906/range2129906/1
type: Range
persistent identity: range2129906
display id: range2129906
version: 1
direction: direction/forward
top level: SbpA | RFP
start: 56
end: 59
orientation: inline

Freiburg scar
type: SequenceAnnotation
persistent identity: annotation2129912
display id: annotation2129912
version: 1
top level: SbpA | RFP
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K525404/annotation2129912/range2129912/1

https://synbiohub.org/public/igem/BBa_K525404/annotation2129912/range2129912/1
type: Range
persistent identity: range2129912
display id: range2129912
version: 1
direction: direction/forward
top level: SbpA | RFP
start: 3193
end: 3198
orientation: inline

T7 promoter
type: SequenceAnnotation
persistent identity: annotation2128864
display id: annotation2128864
version: 1
top level: SbpA | RFP
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K525404/annotation2128864/range2128864/1

https://synbiohub.org/public/igem/BBa_K525404/annotation2128864/range2128864/1
type: Range
persistent identity: range2128864
display id: range2128864
version: 1
direction: direction/forward
top level: SbpA | RFP
start: 1
end: 20
orientation: inline

J18932
type: SequenceAnnotation
persistent identity: annotation2129914
display id: annotation2129914
version: 1
top level: SbpA | RFP
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K525404/annotation2129914/range2129914/1

https://synbiohub.org/public/igem/BBa_K525404/annotation2129914/range2129914/1
type: Range
persistent identity: range2129914
display id: range2129914
version: 1
direction: direction/forward
top level: SbpA | RFP
start: 3199
end: 3912
orientation: inline

EnvZ Tail
type: ComponentDefinition
persistent identity: BBa K1909005
display id: BBa_K1909005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: EnvZ Signal Transduction and Kinase Domains
creator: Daniel Wedemeyer
created: 2016-10-13T11:00:00Z
modified: 2016-10-14T11:30:52Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2375_
m user id: 29525
owner id: 29525
owning group id: 9
status: status/Planning
bookmark: false
mutable description: coming soon
mutable notes: coming soon
mutable provenance: E. coli EnvZ (ncbi: https://www.ncbi.nlm.nih.gov/gene/947272)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: EnvZ
sequence: https://synbiohub.org/public/igem/BBa_K1909005_sequence/1

EnvZ
type: SequenceAnnotation
persistent identity: annotation2507346
display id: annotation2507346
version: 1
top level: EnvZ Tail
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1909005/annotation2507346/range2507346/1

https://synbiohub.org/public/igem/BBa_K1909005/annotation2507346/range2507346/1
type: Range
persistent identity: range2507346
display id: range2507346
version: 1
direction: direction/forward
top level: EnvZ Tail
start: 1
end: 882
orientation: inline

cspB
type: ComponentDefinition
persistent identity: BBa K525222
display id: BBa_K525222
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: S-layer cspB from Corynebacterium halotolerans
creator: Anna Drong
created: 2011-09-10T11:00:00Z
modified: 2015-05-08T01:12:34Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _690_
m user id: 0
owner id: 8543
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: S-layer Corynebacterium halotolerans
mutable notes: PCR
mutable provenance: Corynebacterium halotolerans
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Promoter
sequence annotation: RBS
sequence annotation: cspB
sequence: https://synbiohub.org/public/igem/BBa_K525222_sequence/1

Promoter
type: SequenceAnnotation
persistent identity: annotation2128110
display id: annotation2128110
version: 1
top level: cspB
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K525222/annotation2128110/range2128110/1

https://synbiohub.org/public/igem/BBa_K525222/annotation2128110/range2128110/1
type: Range
persistent identity: range2128110
display id: range2128110
version: 1
direction: direction/forward
top level: cspB
start: 1
end: 22
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2128111
display id: annotation2128111
version: 1
top level: cspB
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K525222/annotation2128111/range2128111/1

https://synbiohub.org/public/igem/BBa_K525222/annotation2128111/range2128111/1
type: Range
persistent identity: range2128111
display id: range2128111
version: 1
direction: direction/forward
top level: cspB
start: 23
end: 34
orientation: inline

cspB
type: SequenceAnnotation
persistent identity: annotation2128112
display id: annotation2128112
version: 1
top level: cspB
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K525222/annotation2128112/range2128112/1

https://synbiohub.org/public/igem/BBa_K525222/annotation2128112/range2128112/1
type: Range
persistent identity: range2128112
display id: range2128112
version: 1
direction: direction/forward
top level: cspB
start: 35
end: 1416
orientation: inline

cspB
type: ComponentDefinition
persistent identity: BBa K525223
display id: BBa_K525223
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: S-layer cspB from Corynebacterium halotolerans with lipid anchor, PT7 and RBS
creator: Anna Drong
created: 2011-09-11T11:00:00Z
modified: 2015-05-08T01:12:34Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _690_
m user id: 0
owner id: 8543
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: S-layer cspB from Corynebacterium halotolerans with TAT-Sequence and lipid anchor, PT7 and RBS
mutable notes: PCR
mutable provenance: Corynebacterium halotolerans
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: lipid anchor
sequence annotation: Promoter
sequence annotation: S-layer
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_K525223_sequence/1

lipid anchor
type: SequenceAnnotation
persistent identity: annotation2127318
display id: annotation2127318
version: 1
top level: cspB
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K525223/annotation2127318/range2127318/1

https://synbiohub.org/public/igem/BBa_K525223/annotation2127318/range2127318/1
type: Range
persistent identity: range2127318
display id: range2127318
version: 1
direction: direction/forward
top level: cspB
start: 1413
end: 1493
orientation: inline

Promoter
type: SequenceAnnotation
persistent identity: annotation2128114
display id: annotation2128114
version: 1
top level: cspB
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K525223/annotation2128114/range2128114/1

https://synbiohub.org/public/igem/BBa_K525223/annotation2128114/range2128114/1
type: Range
persistent identity: range2128114
display id: range2128114
version: 1
direction: direction/forward
top level: cspB
start: 1
end: 20
orientation: inline

S-layer
type: SequenceAnnotation
persistent identity: annotation2128115
display id: annotation2128115
version: 1
top level: cspB
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K525223/annotation2128115/range2128115/1

https://synbiohub.org/public/igem/BBa_K525223/annotation2128115/range2128115/1
type: Range
persistent identity: range2128115
display id: range2128115
version: 1
direction: direction/forward
top level: cspB
start: 33
end: 1412
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2128113
display id: annotation2128113
version: 1
top level: cspB
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K525223/annotation2128113/range2128113/1

https://synbiohub.org/public/igem/BBa_K525223/annotation2128113/range2128113/1
type: Range
persistent identity: range2128113
display id: range2128113
version: 1
direction: direction/forward
top level: cspB
start: 21
end: 32
orientation: inline

BBa_K157006
type: ComponentDefinition
persistent identity: BBa K157006
display id: BBa_K157006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Split-Cerulean-nCFP
creator: iGEM Team Freiburg 2008
created: 2008-10-25T11:00:00Z
modified: 2015-05-08T01:10:54Z
discontinued: false
dominant: false
experience: experience/None
group u list: _232_
m user id: 0
owner id: 1673
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: N-terminal fragment (Amino acids1-155) of the monomeric[5] cyan fluorescent protein variant ???Cerulean???, designed for fusion/BiFC[1,3]. Reassembly with Split-Cerulean-cCFP (Part Bba_K157005) generates the complete, working CFP ???Cerulean???, whereas combination with the C-terminal fragment (Part Bba_K157007) of the monomeric yellow fluorescent Protein ???Venus???[4] (Part Bba_I757008) results in green fluorescence[2]. - [1] Chang-Deng Hu, Yurii Chinenov, Tom K. Kerppola: ???Visualization of Interactions among bZIP and Rel Family Proteins in Living Cells Using Bimolecular Fluorescence Complementation???, Molecular Cell, Vol. 9, 789???798, April, 2002 - [2] Chang Deng Hu, Tom K. Kerppola: ???Simultaneous visualization of multiple protein interactions in living cells using multicolor fluorescence complementation analysis???, Nat Biotechnol. 2003 May; 21(5):539-545 (doi:10. 1038/nbt816) -[3] Tom K. Kerppola: ???Design and implementation of bimolecular fluorescence complementation (BiFC) assays for the visualization of protein interactions in living cells???, Nat Protoc. 2006;1(3):1278-1286 (doi:10.1038/nprot.2006.201) -[4]Nagai, T. et al. ???A variant of yellow fluorescent protein with fast and efficient maturation for cell-biological applications??? J. Biol. Chem. 276, 29188-29194, 2001 -[5]Roger Y. Tsien et al. ???Creating new fluorescent probes for cell biology???, Nature Biotechnology Reviews, Vol. 3, 906-918, 2002
mutable notes: Between BioBrick 1.0 sites this part is flanked 5' by NgoMI(=NgoMIV) and 3' AgeI(=PinAI) to facilitate in frame cloning for protein fusions.
mutable provenance: Gene synthesis by GeneArt, optimized for expression in homo sapiens.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: N-terminal portion of Cerulean CFP
sequence: https://synbiohub.org/public/igem/BBa_K157006_sequence/1

N-terminal portion of Cerulean CFP
type: SequenceAnnotation
persistent identity: annotation2064720
display id: annotation2064720
version: 1
top level: BBa_K157006
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157006/annotation2064720/range2064720/1

https://synbiohub.org/public/igem/BBa_K157006/annotation2064720/range2064720/1
type: Range
persistent identity: range2064720
display id: range2064720
version: 1
direction: direction/forward
top level: BBa_K157006
start: 1
end: 465
orientation: inline

BBa_K525582
type: ComponentDefinition
persistent identity: BBa K525582
display id: BBa_K525582
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Fusion protein of NADP+ Oxidoreductase and BisdA and BisdB, polycistronic
creator: Anna Drong
created: 2011-10-27T11:00:00Z
modified: 2015-05-08T01:12:35Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _690_
m user id: 0
owner id: 8543
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Fusion protein of NADP+ Oxidoreduktase and BisdA and BisdB, policistronic
mutable notes: assembled
mutable provenance: K525499, K12300, K123001
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: ATG
sequence annotation: AgeI
sequence annotation: J23110
sequence annotation: K525515
sequence annotation: B0034
sequence annotation: Freiburg scar
sequence annotation: K525499
sequence annotation: promoter
sequence annotation: Cytochrome P450
sequence annotation: RBS
sequence annotation: Ferredoxin-NADP+ oxidoreductase
sequence annotation: TAA
sequence annotation: NgoMIV
sequence annotation: Ferredoxin
sequence: https://synbiohub.org/public/igem/BBa_K525582_sequence/1

ATG
type: SequenceAnnotation
persistent identity: annotation2161111
display id: annotation2161111
version: 1
top level: BBa_K525582
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K525582/annotation2161111/range2161111/1

https://synbiohub.org/public/igem/BBa_K525582/annotation2161111/range2161111/1
type: Range
persistent identity: range2161111
display id: range2161111
version: 1
direction: direction/forward
top level: BBa_K525582
start: 62
end: 64
orientation: inline

AgeI
type: SequenceAnnotation
persistent identity: annotation2161121
display id: annotation2161121
version: 1
top level: BBa_K525582
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K525582/annotation2161121/range2161121/1

https://synbiohub.org/public/igem/BBa_K525582/annotation2161121/range2161121/1
type: Range
persistent identity: range2161121
display id: range2161121
version: 1
direction: direction/forward
top level: BBa_K525582
start: 2440
end: 2445
orientation: inline

J23110
type: SequenceAnnotation
persistent identity: annotation2161108
display id: annotation2161108
version: 1
top level: BBa_K525582
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K525582/annotation2161108/range2161108/1

https://synbiohub.org/public/igem/BBa_K525582/annotation2161108/range2161108/1
type: Range
persistent identity: range2161108
display id: range2161108
version: 1
direction: direction/forward
top level: BBa_K525582
start: 1
end: 35
orientation: inline

K525515
type: SequenceAnnotation
persistent identity: annotation2161117
display id: annotation2161117
version: 1
top level: BBa_K525582
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K525582/annotation2161117/range2161117/1

https://synbiohub.org/public/igem/BBa_K525582/annotation2161117/range2161117/1
type: Range
persistent identity: range2161117
display id: range2161117
version: 1
direction: direction/forward
top level: BBa_K525582
start: 847
end: 2448
orientation: inline

AgeI
type: SequenceAnnotation
persistent identity: annotation2161114
display id: annotation2161114
version: 1
top level: BBa_K525582
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K525582/annotation2161114/range2161114/1

https://synbiohub.org/public/igem/BBa_K525582/annotation2161114/range2161114/1
type: Range
persistent identity: range2161114
display id: range2161114
version: 1
direction: direction/forward
top level: BBa_K525582
start: 812
end: 817
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation2161109
display id: annotation2161109
version: 1
top level: BBa_K525582
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K525582/annotation2161109/range2161109/1

https://synbiohub.org/public/igem/BBa_K525582/annotation2161109/range2161109/1
type: Range
persistent identity: range2161109
display id: range2161109
version: 1
direction: direction/forward
top level: BBa_K525582
start: 44
end: 55
orientation: inline

ATG
type: SequenceAnnotation
persistent identity: annotation2161118
display id: annotation2161118
version: 1
top level: BBa_K525582
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K525582/annotation2161118/range2161118/1

https://synbiohub.org/public/igem/BBa_K525582/annotation2161118/range2161118/1
type: Range
persistent identity: range2161118
display id: range2161118
version: 1
direction: direction/forward
top level: BBa_K525582
start: 847
end: 849
orientation: inline

Freiburg scar
type: SequenceAnnotation
persistent identity: annotation2161124
display id: annotation2161124
version: 1
top level: BBa_K525582
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K525582/annotation2161124/range2161124/1

https://synbiohub.org/public/igem/BBa_K525582/annotation2161124/range2161124/1
type: Range
persistent identity: range2161124
display id: range2161124
version: 1
direction: direction/forward
top level: BBa_K525582
start: 1168
end: 1173
orientation: inline

K525499
type: SequenceAnnotation
persistent identity: annotation2161110
display id: annotation2161110
version: 1
top level: BBa_K525582
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K525582/annotation2161110/range2161110/1

https://synbiohub.org/public/igem/BBa_K525582/annotation2161110/range2161110/1
type: Range
persistent identity: range2161110
display id: range2161110
version: 1
direction: direction/forward
top level: BBa_K525582
start: 62
end: 820
orientation: inline

promoter
type: SequenceAnnotation
persistent identity: annotation2161106
display id: annotation2161106
version: 1
top level: BBa_K525582
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K525582/annotation2161106/range2161106/1

https://synbiohub.org/public/igem/BBa_K525582/annotation2161106/range2161106/1
type: Range
persistent identity: range2161106
display id: range2161106
version: 1
direction: direction/forward
top level: BBa_K525582
start: 1
end: 35
orientation: inline

Cytochrome P450
type: SequenceAnnotation
persistent identity: annotation2161140
display id: annotation2161140
version: 1
top level: BBa_K525582
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K525582/annotation2161140/range2161140/1

https://synbiohub.org/public/igem/BBa_K525582/annotation2161140/range2161140/1
type: Range
persistent identity: range2161140
display id: range2161140
version: 1
direction: direction/forward
top level: BBa_K525582
start: 1174
end: 2448
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2161107
display id: annotation2161107
version: 1
top level: BBa_K525582
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K525582/annotation2161107/range2161107/1

https://synbiohub.org/public/igem/BBa_K525582/annotation2161107/range2161107/1
type: Range
persistent identity: range2161107
display id: range2161107
version: 1
direction: direction/forward
top level: BBa_K525582
start: 48
end: 51
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2161116
display id: annotation2161116
version: 1
top level: BBa_K525582
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K525582/annotation2161116/range2161116/1

https://synbiohub.org/public/igem/BBa_K525582/annotation2161116/range2161116/1
type: Range
persistent identity: range2161116
display id: range2161116
version: 1
direction: direction/forward
top level: BBa_K525582
start: 834
end: 837
orientation: inline

Ferredoxin-NADP+ oxidoreductase
type: SequenceAnnotation
persistent identity: annotation2161122
display id: annotation2161122
version: 1
top level: BBa_K525582
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K525582/annotation2161122/range2161122/1

https://synbiohub.org/public/igem/BBa_K525582/annotation2161122/range2161122/1
type: Range
persistent identity: range2161122
display id: range2161122
version: 1
direction: direction/forward
top level: BBa_K525582
start: 62
end: 820
orientation: inline

TAA
type: SequenceAnnotation
persistent identity: annotation2161113
display id: annotation2161113
version: 1
top level: BBa_K525582
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K525582/annotation2161113/range2161113/1

https://synbiohub.org/public/igem/BBa_K525582/annotation2161113/range2161113/1
type: Range
persistent identity: range2161113
display id: range2161113
version: 1
direction: direction/forward
top level: BBa_K525582
start: 818
end: 820
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation2161115
display id: annotation2161115
version: 1
top level: BBa_K525582
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K525582/annotation2161115/range2161115/1

https://synbiohub.org/public/igem/BBa_K525582/annotation2161115/range2161115/1
type: Range
persistent identity: range2161115
display id: range2161115
version: 1
direction: direction/forward
top level: BBa_K525582
start: 829
end: 840
orientation: inline

TAA
type: SequenceAnnotation
persistent identity: annotation2161120
display id: annotation2161120
version: 1
top level: BBa_K525582
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K525582/annotation2161120/range2161120/1

https://synbiohub.org/public/igem/BBa_K525582/annotation2161120/range2161120/1
type: Range
persistent identity: range2161120
display id: range2161120
version: 1
direction: direction/forward
top level: BBa_K525582
start: 2446
end: 2448
orientation: inline

NgoMIV
type: SequenceAnnotation
persistent identity: annotation2161119
display id: annotation2161119
version: 1
top level: BBa_K525582
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K525582/annotation2161119/range2161119/1

https://synbiohub.org/public/igem/BBa_K525582/annotation2161119/range2161119/1
type: Range
persistent identity: range2161119
display id: range2161119
version: 1
direction: direction/forward
top level: BBa_K525582
start: 852
end: 857
orientation: inline

Ferredoxin
type: SequenceAnnotation
persistent identity: annotation2161123
display id: annotation2161123
version: 1
top level: BBa_K525582
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K525582/annotation2161123/range2161123/1

https://synbiohub.org/public/igem/BBa_K525582/annotation2161123/range2161123/1
type: Range
persistent identity: range2161123
display id: range2161123
version: 1
direction: direction/forward
top level: BBa_K525582
start: 847
end: 1167
orientation: inline

NgoMIV
type: SequenceAnnotation
persistent identity: annotation2161112
display id: annotation2161112
version: 1
top level: BBa_K525582
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K525582/annotation2161112/range2161112/1

https://synbiohub.org/public/igem/BBa_K525582/annotation2161112/range2161112/1
type: Range
persistent identity: range2161112
display id: range2161112
version: 1
direction: direction/forward
top level: BBa_K525582
start: 65
end: 70
orientation: inline

cspB
type: ComponentDefinition
persistent identity: BBa K525224
display id: BBa_K525224
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: S-layer cspB from Corynebacterium halotolerans with TAT-sequence, PT7 and RBS
creator: Anna Drong
created: 2011-09-11T11:00:00Z
modified: 2015-05-08T01:12:34Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _690_
m user id: 0
owner id: 8543
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: S-layer cspB from Corynebacterium halotolerans with PT7 and RBS
mutable notes: PCR
mutable provenance: Corynebacterium halotolerans
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Promoter
sequence annotation: RBS
sequence annotation: cspB
sequence annotation: B0034
sequence annotation: TAT-sequence
sequence: https://synbiohub.org/public/igem/BBa_K525224_sequence/1

Promoter
type: SequenceAnnotation
persistent identity: annotation2184438
display id: annotation2184438
version: 1
top level: cspB
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K525224/annotation2184438/range2184438/1

https://synbiohub.org/public/igem/BBa_K525224/annotation2184438/range2184438/1
type: Range
persistent identity: range2184438
display id: range2184438
version: 1
direction: direction/forward
top level: cspB
start: 1
end: 22
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2184440
display id: annotation2184440
version: 1
top level: cspB
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K525224/annotation2184440/range2184440/1

https://synbiohub.org/public/igem/BBa_K525224/annotation2184440/range2184440/1
type: Range
persistent identity: range2184440
display id: range2184440
version: 1
direction: direction/forward
top level: cspB
start: 26
end: 29
orientation: inline

cspB
type: SequenceAnnotation
persistent identity: annotation2184442
display id: annotation2184442
version: 1
top level: cspB
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K525224/annotation2184442/range2184442/1

https://synbiohub.org/public/igem/BBa_K525224/annotation2184442/range2184442/1
type: Range
persistent identity: range2184442
display id: range2184442
version: 1
direction: direction/forward
top level: cspB
start: 125
end: 1504
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation2184439
display id: annotation2184439
version: 1
top level: cspB
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K525224/annotation2184439/range2184439/1

https://synbiohub.org/public/igem/BBa_K525224/annotation2184439/range2184439/1
type: Range
persistent identity: range2184439
display id: range2184439
version: 1
direction: direction/forward
top level: cspB
start: 23
end: 34
orientation: inline

TAT-sequence
type: SequenceAnnotation
persistent identity: annotation2184441
display id: annotation2184441
version: 1
top level: cspB
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K525224/annotation2184441/range2184441/1

https://synbiohub.org/public/igem/BBa_K525224/annotation2184441/range2184441/1
type: Range
persistent identity: range2184441
display id: range2184441
version: 1
direction: direction/forward
top level: cspB
start: 34
end: 124
orientation: inline

SP-Lipocal
type: ComponentDefinition
persistent identity: BBa K157034
display id: BBa_K157034
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: SP-Lipocalin-GGGSlinker-Tm-Fluolinker-cYFP
creator: iGEM Team Freiburg 2008
created: 2008-10-25T11:00:00Z
modified: 2015-05-08T01:10:55Z
discontinued: false
dominant: false
experience: experience/None
group u list: _232_
m user id: 0
owner id: 1673
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: A composite part consisting of the signal peptide of Erbb1/EGF-receptor, scFV-antiNIP, GGGSflexlinker (extracellular part), the trans-membrane region of the Erbb1/EGF-receptor and the C-terminal part of the split fluorophor YFP with a fluolinker joined to its N-Terminus (intracellular part).
mutable notes: none
mutable provenance: Derived by cloning of the parts BBa_K157001, BBa_K157004, BBa_K157013, BBa_K157002, BBa_K157009 and BBa_K157007. For further information see the description of the parts mentioned above.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: Fluolinker
sequence annotation: NgoMI
sequence annotation: C-Term_YFP
sequence annotation: Tm
sequence annotation: Lipocalin
sequence annotation: SP
sequence annotation: Scar (Thr, Gly)
sequence annotation: GGGS-Linker
sequence annotation: SP-Lipocalin-GGGSlinker-Tm-Fluolinker-cYFP
sequence annotation: AgeI
sequence: https://synbiohub.org/public/igem/BBa_K157034_sequence/1

Fluolinker
type: SequenceAnnotation
persistent identity: annotation1993358
display id: annotation1993358
version: 1
top level: SP-Lipocal
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157034/annotation1993358/range1993358/1

https://synbiohub.org/public/igem/BBa_K157034/annotation1993358/range1993358/1
type: Range
persistent identity: range1993358
display id: range1993358
version: 1
direction: direction/forward
top level: SP-Lipocal
start: 742
end: 792
orientation: inline

NgoMI
type: SequenceAnnotation
persistent identity: annotation1990276
display id: annotation1990276
version: 1
top level: SP-Lipocal
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157034/annotation1990276/range1990276/1

https://synbiohub.org/public/igem/BBa_K157034/annotation1990276/range1990276/1
type: Range
persistent identity: range1990276
display id: range1990276
version: 1
direction: direction/forward
top level: SP-Lipocal
start: 4
end: 9
orientation: inline

C-Term_YFP
type: SequenceAnnotation
persistent identity: annotation1993360
display id: annotation1993360
version: 1
top level: SP-Lipocal
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157034/annotation1993360/range1993360/1

https://synbiohub.org/public/igem/BBa_K157034/annotation1993360/range1993360/1
type: Range
persistent identity: range1993360
display id: range1993360
version: 1
direction: direction/forward
top level: SP-Lipocal
start: 799
end: 1041
orientation: inline

Tm
type: SequenceAnnotation
persistent identity: annotation1993356
display id: annotation1993356
version: 1
top level: SP-Lipocal
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157034/annotation1993356/range1993356/1

https://synbiohub.org/public/igem/BBa_K157034/annotation1993356/range1993356/1
type: Range
persistent identity: range1993356
display id: range1993356
version: 1
direction: direction/forward
top level: SP-Lipocal
start: 667
end: 735
orientation: inline

Lipocalin
type: SequenceAnnotation
persistent identity: annotation1993352
display id: annotation1993352
version: 1
top level: SP-Lipocal
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157034/annotation1993352/range1993352/1

https://synbiohub.org/public/igem/BBa_K157034/annotation1993352/range1993352/1
type: Range
persistent identity: range1993352
display id: range1993352
version: 1
direction: direction/forward
top level: SP-Lipocal
start: 88
end: 609
orientation: inline

SP
type: SequenceAnnotation
persistent identity: annotation1993350
display id: annotation1993350
version: 1
top level: SP-Lipocal
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157034/annotation1993350/range1993350/1

https://synbiohub.org/public/igem/BBa_K157034/annotation1993350/range1993350/1
type: Range
persistent identity: range1993350
display id: range1993350
version: 1
direction: direction/forward
top level: SP-Lipocal
start: 10
end: 81
orientation: inline

Scar (Thr, Gly)
type: SequenceAnnotation
persistent identity: annotation1993353
display id: annotation1993353
version: 1
top level: SP-Lipocal
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157034/annotation1993353/range1993353/1

https://synbiohub.org/public/igem/BBa_K157034/annotation1993353/range1993353/1
type: Range
persistent identity: range1993353
display id: range1993353
version: 1
direction: direction/forward
top level: SP-Lipocal
start: 610
end: 615
orientation: inline

GGGS-Linker
type: SequenceAnnotation
persistent identity: annotation1993354
display id: annotation1993354
version: 1
top level: SP-Lipocal
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157034/annotation1993354/range1993354/1

https://synbiohub.org/public/igem/BBa_K157034/annotation1993354/range1993354/1
type: Range
persistent identity: range1993354
display id: range1993354
version: 1
direction: direction/forward
top level: SP-Lipocal
start: 616
end: 660
orientation: inline

Scar (Thr, Gly)
type: SequenceAnnotation
persistent identity: annotation1993357
display id: annotation1993357
version: 1
top level: SP-Lipocal
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157034/annotation1993357/range1993357/1

https://synbiohub.org/public/igem/BBa_K157034/annotation1993357/range1993357/1
type: Range
persistent identity: range1993357
display id: range1993357
version: 1
direction: direction/forward
top level: SP-Lipocal
start: 736
end: 741
orientation: inline

Scar (Thr, Gly)
type: SequenceAnnotation
persistent identity: annotation1993351
display id: annotation1993351
version: 1
top level: SP-Lipocal
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157034/annotation1993351/range1993351/1

https://synbiohub.org/public/igem/BBa_K157034/annotation1993351/range1993351/1
type: Range
persistent identity: range1993351
display id: range1993351
version: 1
direction: direction/forward
top level: SP-Lipocal
start: 82
end: 87
orientation: inline

Scar (Thr, Gly)
type: SequenceAnnotation
persistent identity: annotation1993359
display id: annotation1993359
version: 1
top level: SP-Lipocal
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157034/annotation1993359/range1993359/1

https://synbiohub.org/public/igem/BBa_K157034/annotation1993359/range1993359/1
type: Range
persistent identity: range1993359
display id: range1993359
version: 1
direction: direction/forward
top level: SP-Lipocal
start: 793
end: 798
orientation: inline

SP-Lipocalin-GGGSlinker-Tm-Fluolinker-cYFP
type: SequenceAnnotation
persistent identity: annotation1990278
display id: annotation1990278
version: 1
top level: SP-Lipocal
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K157034/annotation1990278/range1990278/1

https://synbiohub.org/public/igem/BBa_K157034/annotation1990278/range1990278/1
type: Range
persistent identity: range1990278
display id: range1990278
version: 1
direction: direction/forward
top level: SP-Lipocal
start: 1
end: 1047
orientation: inline

Scar (Thr, Gly)
type: SequenceAnnotation
persistent identity: annotation1993355
display id: annotation1993355
version: 1
top level: SP-Lipocal
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157034/annotation1993355/range1993355/1

https://synbiohub.org/public/igem/BBa_K157034/annotation1993355/range1993355/1
type: Range
persistent identity: range1993355
display id: range1993355
version: 1
direction: direction/forward
top level: SP-Lipocal
start: 661
end: 666
orientation: inline

AgeI
type: SequenceAnnotation
persistent identity: annotation1990277
display id: annotation1990277
version: 1
top level: SP-Lipocal
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157034/annotation1990277/range1990277/1

https://synbiohub.org/public/igem/BBa_K157034/annotation1990277/range1990277/1
type: Range
persistent identity: range1990277
display id: range1990277
version: 1
direction: direction/forward
top level: SP-Lipocal
start: 1042
end: 1047
orientation: inline

cspB
type: ComponentDefinition
persistent identity: BBa K525121
display id: BBa_K525121
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: S-layer cspB from Corynebacterium glutamicum with TAT-Sequence and lipid anchor, PT7 and RBS
creator: Anna Drong
created: 2011-09-10T11:00:00Z
modified: 2015-05-08T01:12:34Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _690_
m user id: 0
owner id: 8543
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: SL-BF-1 mit pT7 und RBS
mutable notes: PCR
mutable provenance: Brevibacterum flavum
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: C to A
sequence annotation: S-layer
sequence annotation: Promoter
sequence annotation: TAT-sequence
sequence annotation: lipid anchor
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_K525121_sequence/1

C to A
type: SequenceAnnotation
persistent identity: annotation2137388
display id: annotation2137388
version: 1
top level: cspB
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K525121/annotation2137388/range2137388/1

https://synbiohub.org/public/igem/BBa_K525121/annotation2137388/range2137388/1
type: Range
persistent identity: range2137388
display id: range2137388
version: 1
direction: direction/forward
top level: cspB
start: 880
end: 880
orientation: inline

S-layer
type: SequenceAnnotation
persistent identity: annotation2128145
display id: annotation2128145
version: 1
top level: cspB
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K525121/annotation2128145/range2128145/1

https://synbiohub.org/public/igem/BBa_K525121/annotation2128145/range2128145/1
type: Range
persistent identity: range2128145
display id: range2128145
version: 1
direction: direction/forward
top level: cspB
start: 125
end: 1423
orientation: inline

Promoter
type: SequenceAnnotation
persistent identity: annotation2128075
display id: annotation2128075
version: 1
top level: cspB
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K525121/annotation2128075/range2128075/1

https://synbiohub.org/public/igem/BBa_K525121/annotation2128075/range2128075/1
type: Range
persistent identity: range2128075
display id: range2128075
version: 1
direction: direction/forward
top level: cspB
start: 1
end: 22
orientation: inline

TAT-sequence
type: SequenceAnnotation
persistent identity: annotation2128074
display id: annotation2128074
version: 1
top level: cspB
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K525121/annotation2128074/range2128074/1

https://synbiohub.org/public/igem/BBa_K525121/annotation2128074/range2128074/1
type: Range
persistent identity: range2128074
display id: range2128074
version: 1
direction: direction/forward
top level: cspB
start: 35
end: 124
orientation: inline

lipid anchor
type: SequenceAnnotation
persistent identity: annotation2128144
display id: annotation2128144
version: 1
top level: cspB
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K525121/annotation2128144/range2128144/1

https://synbiohub.org/public/igem/BBa_K525121/annotation2128144/range2128144/1
type: Range
persistent identity: range2128144
display id: range2128144
version: 1
direction: direction/forward
top level: cspB
start: 1424
end: 1510
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2127133
display id: annotation2127133
version: 1
top level: cspB
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K525121/annotation2127133/range2127133/1

https://synbiohub.org/public/igem/BBa_K525121/annotation2127133/range2127133/1
type: Range
persistent identity: range2127133
display id: range2127133
version: 1
direction: direction/forward
top level: cspB
start: 23
end: 34
orientation: inline

SgsE|LigA
type: ComponentDefinition
persistent identity: BBa K525005
display id: BBa_K525005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Fusion Protein of S-Layer SgsE and LigA
creator: Anna Drong
created: 2011-09-12T11:00:00Z
modified: 2015-05-08T01:12:34Z
discontinued: false
dominant: false
experience: experience/None
group u list: _690_
m user id: 0
owner id: 8543
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: PT7 + RBS + SgsE || LigA4 ; RCU 25
mutable notes: Assembled
mutable provenance: BBa_K525303, BBa_K525740
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: SgsE domain
sequence annotation: NgoMIV
sequence annotation: TAA
sequence annotation: AgeI
sequence annotation: B0034
sequence annotation: K525301
sequence annotation: RBS
sequence annotation: ATG
sequence annotation: T7 promoter
sequence annotation: Freiburg scar
sequence annotation: K525720
sequence annotation: LigA domain
sequence annotation: lac operator
sequence: https://synbiohub.org/public/igem/BBa_K525005_sequence/1

SgsE domain
type: SequenceAnnotation
persistent identity: annotation2160916
display id: annotation2160916
version: 1
top level: SgsE|LigA
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K525005/annotation2160916/range2160916/1

https://synbiohub.org/public/igem/BBa_K525005/annotation2160916/range2160916/1
type: Range
persistent identity: range2160916
display id: range2160916
version: 1
direction: direction/forward
top level: SgsE|LigA
start: 73
end: 2400
orientation: inline

NgoMIV
type: SequenceAnnotation
persistent identity: annotation2160914
display id: annotation2160914
version: 1
top level: SgsE|LigA
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K525005/annotation2160914/range2160914/1

https://synbiohub.org/public/igem/BBa_K525005/annotation2160914/range2160914/1
type: Range
persistent identity: range2160914
display id: range2160914
version: 1
direction: direction/forward
top level: SgsE|LigA
start: 76
end: 81
orientation: inline

TAA
type: SequenceAnnotation
persistent identity: annotation2160919
display id: annotation2160919
version: 1
top level: SgsE|LigA
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K525005/annotation2160919/range2160919/1

https://synbiohub.org/public/igem/BBa_K525005/annotation2160919/range2160919/1
type: Range
persistent identity: range2160919
display id: range2160919
version: 1
direction: direction/forward
top level: SgsE|LigA
start: 4423
end: 4425
orientation: inline

AgeI
type: SequenceAnnotation
persistent identity: annotation2160918
display id: annotation2160918
version: 1
top level: SgsE|LigA
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K525005/annotation2160918/range2160918/1

https://synbiohub.org/public/igem/BBa_K525005/annotation2160918/range2160918/1
type: Range
persistent identity: range2160918
display id: range2160918
version: 1
direction: direction/forward
top level: SgsE|LigA
start: 4417
end: 4422
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation2160911
display id: annotation2160911
version: 1
top level: SgsE|LigA
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K525005/annotation2160911/range2160911/1

https://synbiohub.org/public/igem/BBa_K525005/annotation2160911/range2160911/1
type: Range
persistent identity: range2160911
display id: range2160911
version: 1
direction: direction/forward
top level: SgsE|LigA
start: 53
end: 64
orientation: inline

K525301
type: SequenceAnnotation
persistent identity: annotation2160915
display id: annotation2160915
version: 1
top level: SgsE|LigA
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K525005/annotation2160915/range2160915/1

https://synbiohub.org/public/igem/BBa_K525005/annotation2160915/range2160915/1
type: Range
persistent identity: range2160915
display id: range2160915
version: 1
direction: direction/forward
top level: SgsE|LigA
start: 73
end: 2400
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2160912
display id: annotation2160912
version: 1
top level: SgsE|LigA
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K525005/annotation2160912/range2160912/1

https://synbiohub.org/public/igem/BBa_K525005/annotation2160912/range2160912/1
type: Range
persistent identity: range2160912
display id: range2160912
version: 1
direction: direction/forward
top level: SgsE|LigA
start: 56
end: 59
orientation: inline

ATG
type: SequenceAnnotation
persistent identity: annotation2160913
display id: annotation2160913
version: 1
top level: SgsE|LigA
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K525005/annotation2160913/range2160913/1

https://synbiohub.org/public/igem/BBa_K525005/annotation2160913/range2160913/1
type: Range
persistent identity: range2160913
display id: range2160913
version: 1
direction: direction/forward
top level: SgsE|LigA
start: 73
end: 75
orientation: inline

T7 promoter
type: SequenceAnnotation
persistent identity: annotation2160909
display id: annotation2160909
version: 1
top level: SgsE|LigA
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K525005/annotation2160909/range2160909/1

https://synbiohub.org/public/igem/BBa_K525005/annotation2160909/range2160909/1
type: Range
persistent identity: range2160909
display id: range2160909
version: 1
direction: direction/forward
top level: SgsE|LigA
start: 1
end: 20
orientation: inline

Freiburg scar
type: SequenceAnnotation
persistent identity: annotation2160917
display id: annotation2160917
version: 1
top level: SgsE|LigA
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K525005/annotation2160917/range2160917/1

https://synbiohub.org/public/igem/BBa_K525005/annotation2160917/range2160917/1
type: Range
persistent identity: range2160917
display id: range2160917
version: 1
direction: direction/forward
top level: SgsE|LigA
start: 2401
end: 2406
orientation: inline

K525720
type: SequenceAnnotation
persistent identity: annotation2160943
display id: annotation2160943
version: 1
top level: SgsE|LigA
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K525005/annotation2160943/range2160943/1

https://synbiohub.org/public/igem/BBa_K525005/annotation2160943/range2160943/1
type: Range
persistent identity: range2160943
display id: range2160943
version: 1
direction: direction/forward
top level: SgsE|LigA
start: 2407
end: 4425
orientation: inline

LigA domain
type: SequenceAnnotation
persistent identity: annotation2161007
display id: annotation2161007
version: 1
top level: SgsE|LigA
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K525005/annotation2161007/range2161007/1

https://synbiohub.org/public/igem/BBa_K525005/annotation2161007/range2161007/1
type: Range
persistent identity: range2161007
display id: range2161007
version: 1
direction: direction/forward
top level: SgsE|LigA
start: 2407
end: 4425
orientation: inline

lac operator
type: SequenceAnnotation
persistent identity: annotation2160910
display id: annotation2160910
version: 1
top level: SgsE|LigA
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K525005/annotation2160910/range2160910/1

https://synbiohub.org/public/igem/BBa_K525005/annotation2160910/range2160910/1
type: Range
persistent identity: range2160910
display id: range2160910
version: 1
direction: direction/forward
top level: SgsE|LigA
start: 20
end: 44
orientation: inline

BBa_K157005
type: ComponentDefinition
persistent identity: BBa K157005
display id: BBa_K157005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Split-Cerulean-cCFP
creator: iGEM Team Freiburg 2008
created: 2008-10-25T11:00:00Z
modified: 2015-05-08T01:10:54Z
discontinued: false
dominant: false
experience: experience/None
group u list: _232_
m user id: 0
owner id: 1673
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: C-terminal fragment (Amino acids156-236) of the monomeric[5] cyan fluorescent protein variant ???Cerulean???, designed for fusion/BiFC[1,3]. Reassembly with Split-Cerulean-nCFP (Part Bba_K157006) generates the complete, working CFP ???Cerulean???, whereas combination with the N-terminal fragment (Part Bba_K157008) of the monomeric yellow fluorescent Protein ???Venus???[4] (Part Bba_I757008) results in green fluorescence[2].References see "Part Design".
mutable notes: Between BioBrick 1.0 sites this part is flanked 5' by NgoMI(=NgoMIV) and 3' AgeI(=PinAI) to facilitate in frame cloning for protein fusions.
mutable provenance: Gene synthesis by GeneArt, optimized for expression in homo sapiens.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: C-Terminal portion of cerulean CFP
sequence: https://synbiohub.org/public/igem/BBa_K157005_sequence/1

C-Terminal portion of cerulean CFP
type: SequenceAnnotation
persistent identity: annotation2064705
display id: annotation2064705
version: 1
top level: BBa_K157005
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157005/annotation2064705/range2064705/1

https://synbiohub.org/public/igem/BBa_K157005/annotation2064705/range2064705/1
type: Range
persistent identity: range2064705
display id: range2064705
version: 1
direction: direction/forward
top level: BBa_K157005
start: 1
end: 243
orientation: inline

LigA
type: ComponentDefinition
persistent identity: BBa K525720
display id: BBa_K525720
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: DNA ligase from Escherichia coli (LigA)
creator: Anna Drong
created: 2011-09-09T11:00:00Z
modified: 2015-05-08T01:12:35Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _690_
m user id: 0
owner id: 8543
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Ligase A im Freiburg Standard
mutable notes: PCR from Escherichia coli
mutable provenance: Escherichia coli
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
sequence annotation: NgoMIV
sequence annotation: AgeI
sequence annotation: TAA
sequence annotation: Lig A
sequence annotation: ATG
sequence: https://synbiohub.org/public/igem/BBa_K525720_sequence/1

NgoMIV
type: SequenceAnnotation
persistent identity: annotation2129482
display id: annotation2129482
version: 1
top level: LigA
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K525720/annotation2129482/range2129482/1

https://synbiohub.org/public/igem/BBa_K525720/annotation2129482/range2129482/1
type: Range
persistent identity: range2129482
display id: range2129482
version: 1
direction: direction/forward
top level: LigA
start: 4
end: 9
orientation: inline

AgeI
type: SequenceAnnotation
persistent identity: annotation2129483
display id: annotation2129483
version: 1
top level: LigA
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K525720/annotation2129483/range2129483/1

https://synbiohub.org/public/igem/BBa_K525720/annotation2129483/range2129483/1
type: Range
persistent identity: range2129483
display id: range2129483
version: 1
direction: direction/forward
top level: LigA
start: 2020
end: 2025
orientation: inline

TAA
type: SequenceAnnotation
persistent identity: annotation2129494
display id: annotation2129494
version: 1
top level: LigA
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K525720/annotation2129494/range2129494/1

https://synbiohub.org/public/igem/BBa_K525720/annotation2129494/range2129494/1
type: Range
persistent identity: range2129494
display id: range2129494
version: 1
direction: direction/forward
top level: LigA
start: 2026
end: 2028
orientation: inline

Lig A
type: SequenceAnnotation
persistent identity: annotation2129504
display id: annotation2129504
version: 1
top level: LigA
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K525720/annotation2129504/range2129504/1

https://synbiohub.org/public/igem/BBa_K525720/annotation2129504/range2129504/1
type: Range
persistent identity: range2129504
display id: range2129504
version: 1
direction: direction/forward
top level: LigA
start: 1
end: 2028
orientation: inline

ATG
type: SequenceAnnotation
persistent identity: annotation2126908
display id: annotation2126908
version: 1
top level: LigA
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K525720/annotation2126908/range2126908/1

https://synbiohub.org/public/igem/BBa_K525720/annotation2126908/range2126908/1
type: Range
persistent identity: range2126908
display id: range2126908
version: 1
direction: direction/forward
top level: LigA
start: 1
end: 3
orientation: inline

SgsE
type: ComponentDefinition
persistent identity: BBa K525301
display id: BBa_K525301
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: S-Layer SgsE from Geobacillus stearothermophilus
creator: Timo Wolf
created: 2011-09-09T11:00:00Z
modified: 2015-05-08T01:12:34Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _690_
m user id: 0
owner id: 6668
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: S-Layer SgsE from Geobacillus stearothermophilus
mutable notes: Synthesis
mutable provenance: Synthesis
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: AgeI
sequence annotation: TAA
sequence annotation: ATG
sequence annotation: SgsE domain
sequence annotation: SgsE
sequence annotation: NgoMIV
sequence: https://synbiohub.org/public/igem/BBa_K525301_sequence/1

AgeI
type: SequenceAnnotation
persistent identity: annotation2129985
display id: annotation2129985
version: 1
top level: SgsE
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K525301/annotation2129985/range2129985/1

https://synbiohub.org/public/igem/BBa_K525301/annotation2129985/range2129985/1
type: Range
persistent identity: range2129985
display id: range2129985
version: 1
direction: direction/forward
top level: SgsE
start: 2329
end: 2334
orientation: inline

TAA
type: SequenceAnnotation
persistent identity: annotation2129984
display id: annotation2129984
version: 1
top level: SgsE
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K525301/annotation2129984/range2129984/1

https://synbiohub.org/public/igem/BBa_K525301/annotation2129984/range2129984/1
type: Range
persistent identity: range2129984
display id: range2129984
version: 1
direction: direction/forward
top level: SgsE
start: 2335
end: 2337
orientation: inline

ATG
type: SequenceAnnotation
persistent identity: annotation2129983
display id: annotation2129983
version: 1
top level: SgsE
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K525301/annotation2129983/range2129983/1

https://synbiohub.org/public/igem/BBa_K525301/annotation2129983/range2129983/1
type: Range
persistent identity: range2129983
display id: range2129983
version: 1
direction: direction/forward
top level: SgsE
start: 1
end: 3
orientation: inline

SgsE domain
type: SequenceAnnotation
persistent identity: annotation2129987
display id: annotation2129987
version: 1
top level: SgsE
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K525301/annotation2129987/range2129987/1

https://synbiohub.org/public/igem/BBa_K525301/annotation2129987/range2129987/1
type: Range
persistent identity: range2129987
display id: range2129987
version: 1
direction: direction/forward
top level: SgsE
start: 1
end: 2337
orientation: inline

SgsE
type: SequenceAnnotation
persistent identity: annotation2128155
display id: annotation2128155
version: 1
top level: SgsE
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K525301/annotation2128155/range2128155/1

https://synbiohub.org/public/igem/BBa_K525301/annotation2128155/range2128155/1
type: Range
persistent identity: range2128155
display id: range2128155
version: 1
direction: direction/forward
top level: SgsE
start: 1
end: 2337
orientation: inline

NgoMIV
type: SequenceAnnotation
persistent identity: annotation2129986
display id: annotation2129986
version: 1
top level: SgsE
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K525301/annotation2129986/range2129986/1

https://synbiohub.org/public/igem/BBa_K525301/annotation2129986/range2129986/1
type: Range
persistent identity: range2129986
display id: range2129986
version: 1
direction: direction/forward
top level: SgsE
start: 4
end: 9
orientation: inline

Luc
type: ComponentDefinition
persistent identity: BBa K525999
display id: BBa_K525999
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Firefly -Luciferase
creator: Anna Drong
created: 2011-09-12T11:00:00Z
modified: 2015-05-08T01:12:35Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _690_
m user id: 0
owner id: 8543
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: Luciferase from pGL3 ; RCU 25
mutable notes: Assembled
mutable provenance: BBa_I712019
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: NgoMIV
sequence annotation: Firefly luciferase
sequence annotation: TAA
sequence annotation: AgeI
sequence annotation: ATG
sequence: https://synbiohub.org/public/igem/BBa_K525999_sequence/1

NgoMIV
type: SequenceAnnotation
persistent identity: annotation2130013
display id: annotation2130013
version: 1
top level: Luc
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K525999/annotation2130013/range2130013/1

https://synbiohub.org/public/igem/BBa_K525999/annotation2130013/range2130013/1
type: Range
persistent identity: range2130013
display id: range2130013
version: 1
direction: direction/forward
top level: Luc
start: 4
end: 9
orientation: inline

Firefly luciferase
type: SequenceAnnotation
persistent identity: annotation2130011
display id: annotation2130011
version: 1
top level: Luc
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K525999/annotation2130011/range2130011/1

https://synbiohub.org/public/igem/BBa_K525999/annotation2130011/range2130011/1
type: Range
persistent identity: range2130011
display id: range2130011
version: 1
direction: direction/forward
top level: Luc
start: 1
end: 1665
orientation: inline

TAA
type: SequenceAnnotation
persistent identity: annotation2130015
display id: annotation2130015
version: 1
top level: Luc
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K525999/annotation2130015/range2130015/1

https://synbiohub.org/public/igem/BBa_K525999/annotation2130015/range2130015/1
type: Range
persistent identity: range2130015
display id: range2130015
version: 1
direction: direction/forward
top level: Luc
start: 1663
end: 1665
orientation: inline

AgeI
type: SequenceAnnotation
persistent identity: annotation2130014
display id: annotation2130014
version: 1
top level: Luc
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K525999/annotation2130014/range2130014/1

https://synbiohub.org/public/igem/BBa_K525999/annotation2130014/range2130014/1
type: Range
persistent identity: range2130014
display id: range2130014
version: 1
direction: direction/forward
top level: Luc
start: 1657
end: 1662
orientation: inline

ATG
type: SequenceAnnotation
persistent identity: annotation2130012
display id: annotation2130012
version: 1
top level: Luc
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K525999/annotation2130012/range2130012/1

https://synbiohub.org/public/igem/BBa_K525999/annotation2130012/range2130012/1
type: Range
persistent identity: range2130012
display id: range2130012
version: 1
direction: direction/forward
top level: Luc
start: 1
end: 3
orientation: inline

BBa_K525002
type: ComponentDefinition
persistent identity: BBa K525002
display id: BBa_K525002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Fusion protein of SbpA, NADP+ Oxidoreductase, BisdA and BisdB with PT7/lac
creator: Anna Drong
created: 2011-09-10T11:00:00Z
modified: 2015-05-08T01:12:34Z
discontinued: false
dominant: false
experience: experience/None
group u list: _690_
m user id: 0
owner id: 8543
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: PT7 + RBS + SbpA || Red || BisdA || BisdB
mutable notes: Assembled
mutable provenance: BBa_K525403; BBa_K525499; BBa_K123000 ; BBa_K123001 ; RCU 25
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: TAA
sequence annotation: Freiburg scar
sequence annotation: B0034
sequence annotation: Freiburg sacr
sequence annotation: RBS
sequence annotation: lac operator
sequence annotation: BisdB
sequence annotation: SbpA domain
sequence annotation: BisdA
sequence annotation: Reductase
sequence annotation: K525560
sequence annotation: T7 promoter
sequence annotation: K525401
sequence annotation: AgeI
sequence annotation: NgoMIV
sequence annotation: ATG
sequence: https://synbiohub.org/public/igem/BBa_K525002_sequence/1

TAA
type: SequenceAnnotation
persistent identity: annotation2160935
display id: annotation2160935
version: 1
top level: BBa_K525002
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K525002/annotation2160935/range2160935/1

https://synbiohub.org/public/igem/BBa_K525002/annotation2160935/range2160935/1
type: Range
persistent identity: range2160935
display id: range2160935
version: 1
direction: direction/forward
top level: BBa_K525002
start: 5536
end: 5538
orientation: inline

Freiburg scar
type: SequenceAnnotation
persistent identity: annotation2160930
display id: annotation2160930
version: 1
top level: BBa_K525002
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K525002/annotation2160930/range2160930/1

https://synbiohub.org/public/igem/BBa_K525002/annotation2160930/range2160930/1
type: Range
persistent identity: range2160930
display id: range2160930
version: 1
direction: direction/forward
top level: BBa_K525002
start: 3940
end: 3945
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation2161005
display id: annotation2161005
version: 1
top level: BBa_K525002
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K525002/annotation2161005/range2161005/1

https://synbiohub.org/public/igem/BBa_K525002/annotation2161005/range2161005/1
type: Range
persistent identity: range2161005
display id: range2161005
version: 1
direction: direction/forward
top level: BBa_K525002
start: 53
end: 64
orientation: inline

Freiburg sacr
type: SequenceAnnotation
persistent identity: annotation2160932
display id: annotation2160932
version: 1
top level: BBa_K525002
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K525002/annotation2160932/range2160932/1

https://synbiohub.org/public/igem/BBa_K525002/annotation2160932/range2160932/1
type: Range
persistent identity: range2160932
display id: range2160932
version: 1
direction: direction/forward
top level: BBa_K525002
start: 4258
end: 4262
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2160923
display id: annotation2160923
version: 1
top level: BBa_K525002
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K525002/annotation2160923/range2160923/1

https://synbiohub.org/public/igem/BBa_K525002/annotation2160923/range2160923/1
type: Range
persistent identity: range2160923
display id: range2160923
version: 1
direction: direction/forward
top level: BBa_K525002
start: 56
end: 59
orientation: inline

lac operator
type: SequenceAnnotation
persistent identity: annotation2160922
display id: annotation2160922
version: 1
top level: BBa_K525002
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K525002/annotation2160922/range2160922/1

https://synbiohub.org/public/igem/BBa_K525002/annotation2160922/range2160922/1
type: Range
persistent identity: range2160922
display id: range2160922
version: 1
direction: direction/forward
top level: BBa_K525002
start: 20
end: 40
orientation: inline

BisdB
type: SequenceAnnotation
persistent identity: annotation2160933
display id: annotation2160933
version: 1
top level: BBa_K525002
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K525002/annotation2160933/range2160933/1

https://synbiohub.org/public/igem/BBa_K525002/annotation2160933/range2160933/1
type: Range
persistent identity: range2160933
display id: range2160933
version: 1
direction: direction/forward
top level: BBa_K525002
start: 4263
end: 5538
orientation: inline

SbpA domain
type: SequenceAnnotation
persistent identity: annotation2160925
display id: annotation2160925
version: 1
top level: BBa_K525002
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K525002/annotation2160925/range2160925/1

https://synbiohub.org/public/igem/BBa_K525002/annotation2160925/range2160925/1
type: Range
persistent identity: range2160925
display id: range2160925
version: 1
direction: direction/forward
top level: BBa_K525002
start: 73
end: 3192
orientation: inline

BisdA
type: SequenceAnnotation
persistent identity: annotation2160931
display id: annotation2160931
version: 1
top level: BBa_K525002
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K525002/annotation2160931/range2160931/1

https://synbiohub.org/public/igem/BBa_K525002/annotation2160931/range2160931/1
type: Range
persistent identity: range2160931
display id: range2160931
version: 1
direction: direction/forward
top level: BBa_K525002
start: 3946
end: 4258
orientation: inline

Reductase
type: SequenceAnnotation
persistent identity: annotation2160928
display id: annotation2160928
version: 1
top level: BBa_K525002
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K525002/annotation2160928/range2160928/1

https://synbiohub.org/public/igem/BBa_K525002/annotation2160928/range2160928/1
type: Range
persistent identity: range2160928
display id: range2160928
version: 1
direction: direction/forward
top level: BBa_K525002
start: 3199
end: 3939
orientation: inline

K525560
type: SequenceAnnotation
persistent identity: annotation2160929
display id: annotation2160929
version: 1
top level: BBa_K525002
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K525002/annotation2160929/range2160929/1

https://synbiohub.org/public/igem/BBa_K525002/annotation2160929/range2160929/1
type: Range
persistent identity: range2160929
display id: range2160929
version: 1
direction: direction/forward
top level: BBa_K525002
start: 3199
end: 5538
orientation: inline

T7 promoter
type: SequenceAnnotation
persistent identity: annotation2160920
display id: annotation2160920
version: 1
top level: BBa_K525002
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K525002/annotation2160920/range2160920/1

https://synbiohub.org/public/igem/BBa_K525002/annotation2160920/range2160920/1
type: Range
persistent identity: range2160920
display id: range2160920
version: 1
direction: direction/forward
top level: BBa_K525002
start: 1
end: 20
orientation: inline

K525401
type: SequenceAnnotation
persistent identity: annotation2160921
display id: annotation2160921
version: 1
top level: BBa_K525002
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K525002/annotation2160921/range2160921/1

https://synbiohub.org/public/igem/BBa_K525002/annotation2160921/range2160921/1
type: Range
persistent identity: range2160921
display id: range2160921
version: 1
direction: direction/forward
top level: BBa_K525002
start: 73
end: 3192
orientation: inline

AgeI
type: SequenceAnnotation
persistent identity: annotation2160934
display id: annotation2160934
version: 1
top level: BBa_K525002
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K525002/annotation2160934/range2160934/1

https://synbiohub.org/public/igem/BBa_K525002/annotation2160934/range2160934/1
type: Range
persistent identity: range2160934
display id: range2160934
version: 1
direction: direction/forward
top level: BBa_K525002
start: 5530
end: 5535
orientation: inline

NgoMIV
type: SequenceAnnotation
persistent identity: annotation2160926
display id: annotation2160926
version: 1
top level: BBa_K525002
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K525002/annotation2160926/range2160926/1

https://synbiohub.org/public/igem/BBa_K525002/annotation2160926/range2160926/1
type: Range
persistent identity: range2160926
display id: range2160926
version: 1
direction: direction/forward
top level: BBa_K525002
start: 76
end: 81
orientation: inline

Freiburg scar
type: SequenceAnnotation
persistent identity: annotation2160927
display id: annotation2160927
version: 1
top level: BBa_K525002
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K525002/annotation2160927/range2160927/1

https://synbiohub.org/public/igem/BBa_K525002/annotation2160927/range2160927/1
type: Range
persistent identity: range2160927
display id: range2160927
version: 1
direction: direction/forward
top level: BBa_K525002
start: 3193
end: 3198
orientation: inline

ATG
type: SequenceAnnotation
persistent identity: annotation2160924
display id: annotation2160924
version: 1
top level: BBa_K525002
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K525002/annotation2160924/range2160924/1

https://synbiohub.org/public/igem/BBa_K525002/annotation2160924/range2160924/1
type: Range
persistent identity: range2160924
display id: range2160924
version: 1
direction: direction/forward
top level: BBa_K525002
start: 73
end: 75
orientation: inline

SP-Lipocal
type: ComponentDefinition
persistent identity: BBa K157037
display id: BBa_K157037
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: SP-Lipocalin-GGGSlinker-Tm-nCFP
creator: iGEM Team Freiburg 2008
created: 2008-10-25T11:00:00Z
modified: 2015-05-08T01:10:55Z
discontinued: false
dominant: false
experience: experience/None
group u list: _232_
m user id: 0
owner id: 1673
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: A composite part consisting of the signal peptide of Erbb1/EGF-receptor, scFV-antiNIP, GGGSflexlinker (extracellular part), the trans-membrane region of the Erbb1/EGF-receptor and the C-terminal part of the split fluorophor YFP with a fluolinker joined to its N-Terminus (intracellular part).
mutable notes: none
mutable provenance: Derived by cloning of the parts BBa_K157001, BBa_K157004, BBa_K157013, BBa_K157002, BBa_K157006. For further information see the description of the parts mentioned above.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: Scar (Thr, Gly)
sequence annotation: Lipocalin
sequence annotation: GGGS-Linker
sequence annotation: NgoMI
sequence annotation: N-Term_CFP
sequence annotation: SP-Lipocalin-GGGSlinker-Tm-nCFP
sequence annotation: SP
sequence annotation: AgeI
sequence annotation: Tm
sequence: https://synbiohub.org/public/igem/BBa_K157037_sequence/1

Scar (Thr, Gly)
type: SequenceAnnotation
persistent identity: annotation1993412
display id: annotation1993412
version: 1
top level: SP-Lipocal
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157037/annotation1993412/range1993412/1

https://synbiohub.org/public/igem/BBa_K157037/annotation1993412/range1993412/1
type: Range
persistent identity: range1993412
display id: range1993412
version: 1
direction: direction/forward
top level: SP-Lipocal
start: 610
end: 615
orientation: inline

Lipocalin
type: SequenceAnnotation
persistent identity: annotation1993411
display id: annotation1993411
version: 1
top level: SP-Lipocal
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157037/annotation1993411/range1993411/1

https://synbiohub.org/public/igem/BBa_K157037/annotation1993411/range1993411/1
type: Range
persistent identity: range1993411
display id: range1993411
version: 1
direction: direction/forward
top level: SP-Lipocal
start: 88
end: 609
orientation: inline

Scar (Thr, Gly)
type: SequenceAnnotation
persistent identity: annotation1993414
display id: annotation1993414
version: 1
top level: SP-Lipocal
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157037/annotation1993414/range1993414/1

https://synbiohub.org/public/igem/BBa_K157037/annotation1993414/range1993414/1
type: Range
persistent identity: range1993414
display id: range1993414
version: 1
direction: direction/forward
top level: SP-Lipocal
start: 661
end: 666
orientation: inline

GGGS-Linker
type: SequenceAnnotation
persistent identity: annotation1993413
display id: annotation1993413
version: 1
top level: SP-Lipocal
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157037/annotation1993413/range1993413/1

https://synbiohub.org/public/igem/BBa_K157037/annotation1993413/range1993413/1
type: Range
persistent identity: range1993413
display id: range1993413
version: 1
direction: direction/forward
top level: SP-Lipocal
start: 616
end: 660
orientation: inline

NgoMI
type: SequenceAnnotation
persistent identity: annotation1990297
display id: annotation1990297
version: 1
top level: SP-Lipocal
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157037/annotation1990297/range1990297/1

https://synbiohub.org/public/igem/BBa_K157037/annotation1990297/range1990297/1
type: Range
persistent identity: range1990297
display id: range1990297
version: 1
direction: direction/forward
top level: SP-Lipocal
start: 4
end: 9
orientation: inline

N-Term_CFP
type: SequenceAnnotation
persistent identity: annotation1993417
display id: annotation1993417
version: 1
top level: SP-Lipocal
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157037/annotation1993417/range1993417/1

https://synbiohub.org/public/igem/BBa_K157037/annotation1993417/range1993417/1
type: Range
persistent identity: range1993417
display id: range1993417
version: 1
direction: direction/forward
top level: SP-Lipocal
start: 742
end: 1206
orientation: inline

SP-Lipocalin-GGGSlinker-Tm-nCFP
type: SequenceAnnotation
persistent identity: annotation1990299
display id: annotation1990299
version: 1
top level: SP-Lipocal
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K157037/annotation1990299/range1990299/1

https://synbiohub.org/public/igem/BBa_K157037/annotation1990299/range1990299/1
type: Range
persistent identity: range1990299
display id: range1990299
version: 1
direction: direction/forward
top level: SP-Lipocal
start: 1
end: 1212
orientation: inline

SP
type: SequenceAnnotation
persistent identity: annotation1993409
display id: annotation1993409
version: 1
top level: SP-Lipocal
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157037/annotation1993409/range1993409/1

https://synbiohub.org/public/igem/BBa_K157037/annotation1993409/range1993409/1
type: Range
persistent identity: range1993409
display id: range1993409
version: 1
direction: direction/forward
top level: SP-Lipocal
start: 10
end: 81
orientation: inline

Scar (Thr, Gly)
type: SequenceAnnotation
persistent identity: annotation1993416
display id: annotation1993416
version: 1
top level: SP-Lipocal
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157037/annotation1993416/range1993416/1

https://synbiohub.org/public/igem/BBa_K157037/annotation1993416/range1993416/1
type: Range
persistent identity: range1993416
display id: range1993416
version: 1
direction: direction/forward
top level: SP-Lipocal
start: 736
end: 741
orientation: inline

Scar (Thr, Gly)
type: SequenceAnnotation
persistent identity: annotation1993410
display id: annotation1993410
version: 1
top level: SP-Lipocal
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157037/annotation1993410/range1993410/1

https://synbiohub.org/public/igem/BBa_K157037/annotation1993410/range1993410/1
type: Range
persistent identity: range1993410
display id: range1993410
version: 1
direction: direction/forward
top level: SP-Lipocal
start: 82
end: 87
orientation: inline

AgeI
type: SequenceAnnotation
persistent identity: annotation1990298
display id: annotation1990298
version: 1
top level: SP-Lipocal
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157037/annotation1990298/range1990298/1

https://synbiohub.org/public/igem/BBa_K157037/annotation1990298/range1990298/1
type: Range
persistent identity: range1990298
display id: range1990298
version: 1
direction: direction/forward
top level: SP-Lipocal
start: 1207
end: 1212
orientation: inline

Tm
type: SequenceAnnotation
persistent identity: annotation1993415
display id: annotation1993415
version: 1
top level: SP-Lipocal
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157037/annotation1993415/range1993415/1

https://synbiohub.org/public/igem/BBa_K157037/annotation1993415/range1993415/1
type: Range
persistent identity: range1993415
display id: range1993415
version: 1
direction: direction/forward
top level: SP-Lipocal
start: 667
end: 735
orientation: inline

BBa_K525010
type: ComponentDefinition
persistent identity: BBa K525010
display id: BBa_K525010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Fusion Protein of S-Layer SbpA and LigA with His-Tag
creator: Manuel Wittchen
created: 2011-10-24T11:00:00Z
modified: 2015-05-08T01:12:34Z
discontinued: false
dominant: false
group u list: _690_
m user id: 0
owner id: 8718
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Fusion Protein of S-Layer SbpA and LigA with His-Tag
mutable notes: Assembled with Freiburg Standard Assembly (RFC 25)
mutable provenance: SbpA was synthesized, LigA is the native ligase of E. coli.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Freiburg scar
sequence annotation: ATG
sequence annotation: His-Tag
sequence annotation: B0034
sequence annotation: K525740
sequence annotation: T7 Promotor
sequence annotation: NgoMIV
sequence annotation: lac operator
sequence annotation: SbpA domain
sequence annotation: LigA domain
sequence annotation: K525403
sequence: https://synbiohub.org/public/igem/BBa_K525010_sequence/1

Freiburg scar
type: SequenceAnnotation
persistent identity: annotation2160523
display id: annotation2160523
version: 1
top level: BBa_K525010
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K525010/annotation2160523/range2160523/1

https://synbiohub.org/public/igem/BBa_K525010/annotation2160523/range2160523/1
type: Range
persistent identity: range2160523
display id: range2160523
version: 1
direction: direction/forward
top level: BBa_K525010
start: 3193
end: 3199
orientation: inline

ATG
type: SequenceAnnotation
persistent identity: annotation2160522
display id: annotation2160522
version: 1
top level: BBa_K525010
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K525010/annotation2160522/range2160522/1

https://synbiohub.org/public/igem/BBa_K525010/annotation2160522/range2160522/1
type: Range
persistent identity: range2160522
display id: range2160522
version: 1
direction: direction/forward
top level: BBa_K525010
start: 73
end: 75
orientation: inline

His-Tag
type: SequenceAnnotation
persistent identity: annotation2160526
display id: annotation2160526
version: 1
top level: BBa_K525010
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K525010/annotation2160526/range2160526/1

https://synbiohub.org/public/igem/BBa_K525010/annotation2160526/range2160526/1
type: Range
persistent identity: range2160526
display id: range2160526
version: 1
direction: direction/forward
top level: BBa_K525010
start: 5209
end: 5226
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation2160521
display id: annotation2160521
version: 1
top level: BBa_K525010
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K525010/annotation2160521/range2160521/1

https://synbiohub.org/public/igem/BBa_K525010/annotation2160521/range2160521/1
type: Range
persistent identity: range2160521
display id: range2160521
version: 1
direction: direction/forward
top level: BBa_K525010
start: 53
end: 64
orientation: inline

K525740
type: SequenceAnnotation
persistent identity: annotation2160528
display id: annotation2160528
version: 1
top level: BBa_K525010
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K525010/annotation2160528/range2160528/1

https://synbiohub.org/public/igem/BBa_K525010/annotation2160528/range2160528/1
type: Range
persistent identity: range2160528
display id: range2160528
version: 1
direction: direction/forward
top level: BBa_K525010
start: 3189
end: 5226
orientation: inline

T7 Promotor
type: SequenceAnnotation
persistent identity: annotation2160519
display id: annotation2160519
version: 1
top level: BBa_K525010
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K525010/annotation2160519/range2160519/1

https://synbiohub.org/public/igem/BBa_K525010/annotation2160519/range2160519/1
type: Range
persistent identity: range2160519
display id: range2160519
version: 1
direction: direction/forward
top level: BBa_K525010
start: 1
end: 20
orientation: inline

NgoMIV
type: SequenceAnnotation
persistent identity: annotation2160529
display id: annotation2160529
version: 1
top level: BBa_K525010
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K525010/annotation2160529/range2160529/1

https://synbiohub.org/public/igem/BBa_K525010/annotation2160529/range2160529/1
type: Range
persistent identity: range2160529
display id: range2160529
version: 1
direction: direction/forward
top level: BBa_K525010
start: 76
end: 81
orientation: inline

lac operator
type: SequenceAnnotation
persistent identity: annotation2160520
display id: annotation2160520
version: 1
top level: BBa_K525010
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K525010/annotation2160520/range2160520/1

https://synbiohub.org/public/igem/BBa_K525010/annotation2160520/range2160520/1
type: Range
persistent identity: range2160520
display id: range2160520
version: 1
direction: direction/forward
top level: BBa_K525010
start: 20
end: 44
orientation: inline

SbpA domain
type: SequenceAnnotation
persistent identity: annotation2160524
display id: annotation2160524
version: 1
top level: BBa_K525010
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K525010/annotation2160524/range2160524/1

https://synbiohub.org/public/igem/BBa_K525010/annotation2160524/range2160524/1
type: Range
persistent identity: range2160524
display id: range2160524
version: 1
direction: direction/forward
top level: BBa_K525010
start: 73
end: 3202
orientation: inline

LigA domain
type: SequenceAnnotation
persistent identity: annotation2160525
display id: annotation2160525
version: 1
top level: BBa_K525010
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K525010/annotation2160525/range2160525/1

https://synbiohub.org/public/igem/BBa_K525010/annotation2160525/range2160525/1
type: Range
persistent identity: range2160525
display id: range2160525
version: 1
direction: direction/forward
top level: BBa_K525010
start: 3199
end: 5208
orientation: inline

K525403
type: SequenceAnnotation
persistent identity: annotation2160527
display id: annotation2160527
version: 1
top level: BBa_K525010
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K525010/annotation2160527/range2160527/1

https://synbiohub.org/public/igem/BBa_K525010/annotation2160527/range2160527/1
type: Range
persistent identity: range2160527
display id: range2160527
version: 1
direction: direction/forward
top level: BBa_K525010
start: 1
end: 3193
orientation: inline

LigA
type: ComponentDefinition
persistent identity: BBa K525730
display id: BBa_K525730
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: DNA ligase from Escherichia coli (LigA) with PT7 and RBS
creator: Anna Drong
created: 2011-09-11T11:00:00Z
modified: 2015-05-08T01:12:35Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _690_
m user id: 0
owner id: 8543
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: LigA3 Promotor, RBS, Freiburg Suffix
mutable notes: PCR
mutable provenance: Escherichia coli
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: B0034
sequence annotation: ATG
sequence annotation: AgeI
sequence annotation: LigA
sequence annotation: RBS
sequence annotation: TAA
sequence annotation: T7 promoter
sequence: https://synbiohub.org/public/igem/BBa_K525730_sequence/1

B0034
type: SequenceAnnotation
persistent identity: annotation2137450
display id: annotation2137450
version: 1
top level: LigA
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K525730/annotation2137450/range2137450/1

https://synbiohub.org/public/igem/BBa_K525730/annotation2137450/range2137450/1
type: Range
persistent identity: range2137450
display id: range2137450
version: 1
direction: direction/forward
top level: LigA
start: 21
end: 33
orientation: inline

ATG
type: SequenceAnnotation
persistent identity: annotation2161298
display id: annotation2161298
version: 1
top level: LigA
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K525730/annotation2161298/range2161298/1

https://synbiohub.org/public/igem/BBa_K525730/annotation2161298/range2161298/1
type: Range
persistent identity: range2161298
display id: range2161298
version: 1
direction: direction/forward
top level: LigA
start: 34
end: 36
orientation: inline

AgeI
type: SequenceAnnotation
persistent identity: annotation2137444
display id: annotation2137444
version: 1
top level: LigA
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K525730/annotation2137444/range2137444/1

https://synbiohub.org/public/igem/BBa_K525730/annotation2137444/range2137444/1
type: Range
persistent identity: range2137444
display id: range2137444
version: 1
direction: direction/forward
top level: LigA
start: 2047
end: 2052
orientation: inline

LigA
type: SequenceAnnotation
persistent identity: annotation2137451
display id: annotation2137451
version: 1
top level: LigA
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K525730/annotation2137451/range2137451/1

https://synbiohub.org/public/igem/BBa_K525730/annotation2137451/range2137451/1
type: Range
persistent identity: range2137451
display id: range2137451
version: 1
direction: direction/forward
top level: LigA
start: 34
end: 2055
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2161297
display id: annotation2161297
version: 1
top level: LigA
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K525730/annotation2161297/range2161297/1

https://synbiohub.org/public/igem/BBa_K525730/annotation2161297/range2161297/1
type: Range
persistent identity: range2161297
display id: range2161297
version: 1
direction: direction/forward
top level: LigA
start: 24
end: 27
orientation: inline

TAA
type: SequenceAnnotation
persistent identity: annotation2137445
display id: annotation2137445
version: 1
top level: LigA
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K525730/annotation2137445/range2137445/1

https://synbiohub.org/public/igem/BBa_K525730/annotation2137445/range2137445/1
type: Range
persistent identity: range2137445
display id: range2137445
version: 1
direction: direction/forward
top level: LigA
start: 2053
end: 2055
orientation: inline

T7 promoter
type: SequenceAnnotation
persistent identity: annotation2137443
display id: annotation2137443
version: 1
top level: LigA
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K525730/annotation2137443/range2137443/1

https://synbiohub.org/public/igem/BBa_K525730/annotation2137443/range2137443/1
type: Range
persistent identity: range2137443
display id: range2137443
version: 1
direction: direction/forward
top level: LigA
start: 1
end: 20
orientation: inline

Tm-betaLac
type: ComponentDefinition
persistent identity: BBa K157018
display id: BBa_K157018
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Tm-betaLactamaseBla1
creator: iGEM Team Freiburg 2008
created: 2008-10-25T11:00:00Z
modified: 2015-05-08T01:10:54Z
discontinued: false
dominant: false
experience: experience/None
group u list: _232_
m user id: 0
owner id: 1673
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: First fragment of beta-Lactamase fused to the trans-membrane region of the Erbb1/EGF-receptor.
mutable notes: none
mutable provenance: Derived by cloning of the parts BBa_K157002, BBa_I757011. For further information see the description of the parts mentioned above.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: Scar (Thr, Gly)
sequence annotation: Tm-betaLactamase(Bla1)
sequence annotation: NgoMI
sequence annotation: AgeI
sequence annotation: Tm
sequence annotation: Bla1
sequence: https://synbiohub.org/public/igem/BBa_K157018_sequence/1

Scar (Thr, Gly)
type: SequenceAnnotation
persistent identity: annotation1991372
display id: annotation1991372
version: 1
top level: Tm-betaLac
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157018/annotation1991372/range1991372/1

https://synbiohub.org/public/igem/BBa_K157018/annotation1991372/range1991372/1
type: Range
persistent identity: range1991372
display id: range1991372
version: 1
direction: direction/forward
top level: Tm-betaLac
start: 79
end: 84
orientation: inline

Tm-betaLactamase(Bla1)
type: SequenceAnnotation
persistent identity: annotation1989985
display id: annotation1989985
version: 1
top level: Tm-betaLac
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K157018/annotation1989985/range1989985/1

https://synbiohub.org/public/igem/BBa_K157018/annotation1989985/range1989985/1
type: Range
persistent identity: range1989985
display id: range1989985
version: 1
direction: direction/forward
top level: Tm-betaLac
start: 1
end: 615
orientation: inline

NgoMI
type: SequenceAnnotation
persistent identity: annotation1989983
display id: annotation1989983
version: 1
top level: Tm-betaLac
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157018/annotation1989983/range1989983/1

https://synbiohub.org/public/igem/BBa_K157018/annotation1989983/range1989983/1
type: Range
persistent identity: range1989983
display id: range1989983
version: 1
direction: direction/forward
top level: Tm-betaLac
start: 4
end: 9
orientation: inline

AgeI
type: SequenceAnnotation
persistent identity: annotation1989984
display id: annotation1989984
version: 1
top level: Tm-betaLac
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157018/annotation1989984/range1989984/1

https://synbiohub.org/public/igem/BBa_K157018/annotation1989984/range1989984/1
type: Range
persistent identity: range1989984
display id: range1989984
version: 1
direction: direction/forward
top level: Tm-betaLac
start: 610
end: 615
orientation: inline

Tm
type: SequenceAnnotation
persistent identity: annotation1991288
display id: annotation1991288
version: 1
top level: Tm-betaLac
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157018/annotation1991288/range1991288/1

https://synbiohub.org/public/igem/BBa_K157018/annotation1991288/range1991288/1
type: Range
persistent identity: range1991288
display id: range1991288
version: 1
direction: direction/forward
top level: Tm-betaLac
start: 10
end: 78
orientation: inline

Bla1
type: SequenceAnnotation
persistent identity: annotation1991421
display id: annotation1991421
version: 1
top level: Tm-betaLac
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157018/annotation1991421/range1991421/1

https://synbiohub.org/public/igem/BBa_K157018/annotation1991421/range1991421/1
type: Range
persistent identity: range1991421
display id: range1991421
version: 1
direction: direction/forward
top level: Tm-betaLac
start: 85
end: 609
orientation: inline

SP-Lipocal
type: ComponentDefinition
persistent identity: BBa K157023
display id: BBa_K157023
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: SP-Lipocalin-GGGSlinker
creator: iGEM Team Freiburg 2008
created: 2008-10-25T11:00:00Z
modified: 2015-05-08T01:10:55Z
discontinued: false
dominant: false
experience: experience/None
group u list: _232_
m user id: 0
owner id: 1673
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Lipocalin (with a flexible GGGS-linker at the C-terminus) fusioned to the signal peptide of Erbb1/EGF-receptor.
mutable notes: none
mutable provenance: Derived by cloning of the parts BBa_K157001, BBa_K157003 and BBa_K157013. For further information see the description of the parts mentioned above.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: Scar (Thr, Gly)
sequence annotation: Lipocalin
sequence annotation: SP
sequence annotation: AgeI
sequence annotation: NgoMI
sequence annotation: GGGS-Linker
sequence annotation: SP-Lipocalin-GGGSlinker
sequence: https://synbiohub.org/public/igem/BBa_K157023_sequence/1

Scar (Thr, Gly)
type: SequenceAnnotation
persistent identity: annotation1991820
display id: annotation1991820
version: 1
top level: SP-Lipocal
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157023/annotation1991820/range1991820/1

https://synbiohub.org/public/igem/BBa_K157023/annotation1991820/range1991820/1
type: Range
persistent identity: range1991820
display id: range1991820
version: 1
direction: direction/forward
top level: SP-Lipocal
start: 82
end: 87
orientation: inline

Lipocalin
type: SequenceAnnotation
persistent identity: annotation1991821
display id: annotation1991821
version: 1
top level: SP-Lipocal
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157023/annotation1991821/range1991821/1

https://synbiohub.org/public/igem/BBa_K157023/annotation1991821/range1991821/1
type: Range
persistent identity: range1991821
display id: range1991821
version: 1
direction: direction/forward
top level: SP-Lipocal
start: 88
end: 609
orientation: inline

SP
type: SequenceAnnotation
persistent identity: annotation1991819
display id: annotation1991819
version: 1
top level: SP-Lipocal
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157023/annotation1991819/range1991819/1

https://synbiohub.org/public/igem/BBa_K157023/annotation1991819/range1991819/1
type: Range
persistent identity: range1991819
display id: range1991819
version: 1
direction: direction/forward
top level: SP-Lipocal
start: 10
end: 81
orientation: inline

AgeI
type: SequenceAnnotation
persistent identity: annotation1990164
display id: annotation1990164
version: 1
top level: SP-Lipocal
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157023/annotation1990164/range1990164/1

https://synbiohub.org/public/igem/BBa_K157023/annotation1990164/range1990164/1
type: Range
persistent identity: range1990164
display id: range1990164
version: 1
direction: direction/forward
top level: SP-Lipocal
start: 661
end: 666
orientation: inline

NgoMI
type: SequenceAnnotation
persistent identity: annotation1990163
display id: annotation1990163
version: 1
top level: SP-Lipocal
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157023/annotation1990163/range1990163/1

https://synbiohub.org/public/igem/BBa_K157023/annotation1990163/range1990163/1
type: Range
persistent identity: range1990163
display id: range1990163
version: 1
direction: direction/forward
top level: SP-Lipocal
start: 4
end: 9
orientation: inline

GGGS-Linker
type: SequenceAnnotation
persistent identity: annotation1991826
display id: annotation1991826
version: 1
top level: SP-Lipocal
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157023/annotation1991826/range1991826/1

https://synbiohub.org/public/igem/BBa_K157023/annotation1991826/range1991826/1
type: Range
persistent identity: range1991826
display id: range1991826
version: 1
direction: direction/forward
top level: SP-Lipocal
start: 616
end: 660
orientation: inline

SP-Lipocalin-GGGSlinker
type: SequenceAnnotation
persistent identity: annotation1990165
display id: annotation1990165
version: 1
top level: SP-Lipocal
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K157023/annotation1990165/range1990165/1

https://synbiohub.org/public/igem/BBa_K157023/annotation1990165/range1990165/1
type: Range
persistent identity: range1990165
display id: range1990165
version: 1
direction: direction/forward
top level: SP-Lipocal
start: 1
end: 666
orientation: inline

Scar (Thr, Gly)
type: SequenceAnnotation
persistent identity: annotation1991825
display id: annotation1991825
version: 1
top level: SP-Lipocal
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157023/annotation1991825/range1991825/1

https://synbiohub.org/public/igem/BBa_K157023/annotation1991825/range1991825/1
type: Range
persistent identity: range1991825
display id: range1991825
version: 1
direction: direction/forward
top level: SP-Lipocal
start: 610
end: 615
orientation: inline

BBa_K1486052
type: ComponentDefinition
persistent identity: BBa K1486052
display id: BBa_K1486052
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Spacer
creator: EPFL iGem team 2014
created: 2014-10-06T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: to edit
mutable notes: to edit
mutable provenance: to edit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1486052_sequence/1

BBa_K525007
type: ComponentDefinition
persistent identity: BBa K525007
display id: BBa_K525007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Fusion protein of S-Layer SgsE with T7/lac and BisdA and BisdB
creator: Anna Drong
created: 2011-09-11T11:00:00Z
modified: 2015-05-08T01:12:34Z
discontinued: false
dominant: false
experience: experience/None
group u list: _690_
m user id: 0
owner id: 8543
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: PT7 + RBS + SgsE || BisdA || BisdB
mutable notes: Assembled
mutable provenance: BBa_K525303, BBa_K123000, BBa_K123001
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: BisdB
sequence annotation: AgeI
sequence annotation: TAA
sequence annotation: ATG
sequence annotation: RBS
sequence annotation: K525515
sequence annotation: Freiburg Scar
sequence annotation: K525301
sequence annotation: T7
sequence annotation: B0034
sequence annotation: lac operator
sequence annotation: SgsE domain
sequence annotation: NgoMIV
sequence annotation: BisdA
sequence: https://synbiohub.org/public/igem/BBa_K525007_sequence/1

BisdB
type: SequenceAnnotation
persistent identity: annotation2161174
display id: annotation2161174
version: 1
top level: BBa_K525007
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K525007/annotation2161174/range2161174/1

https://synbiohub.org/public/igem/BBa_K525007/annotation2161174/range2161174/1
type: Range
persistent identity: range2161174
display id: range2161174
version: 1
direction: direction/forward
top level: BBa_K525007
start: 2725
end: 3999
orientation: inline

AgeI
type: SequenceAnnotation
persistent identity: annotation2161175
display id: annotation2161175
version: 1
top level: BBa_K525007
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K525007/annotation2161175/range2161175/1

https://synbiohub.org/public/igem/BBa_K525007/annotation2161175/range2161175/1
type: Range
persistent identity: range2161175
display id: range2161175
version: 1
direction: direction/forward
top level: BBa_K525007
start: 3991
end: 3996
orientation: inline

TAA
type: SequenceAnnotation
persistent identity: annotation2161176
display id: annotation2161176
version: 1
top level: BBa_K525007
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K525007/annotation2161176/range2161176/1

https://synbiohub.org/public/igem/BBa_K525007/annotation2161176/range2161176/1
type: Range
persistent identity: range2161176
display id: range2161176
version: 1
direction: direction/forward
top level: BBa_K525007
start: 3997
end: 3999
orientation: inline

ATG
type: SequenceAnnotation
persistent identity: annotation2161166
display id: annotation2161166
version: 1
top level: BBa_K525007
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K525007/annotation2161166/range2161166/1

https://synbiohub.org/public/igem/BBa_K525007/annotation2161166/range2161166/1
type: Range
persistent identity: range2161166
display id: range2161166
version: 1
direction: direction/forward
top level: BBa_K525007
start: 73
end: 75
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2161165
display id: annotation2161165
version: 1
top level: BBa_K525007
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K525007/annotation2161165/range2161165/1

https://synbiohub.org/public/igem/BBa_K525007/annotation2161165/range2161165/1
type: Range
persistent identity: range2161165
display id: range2161165
version: 1
direction: direction/forward
top level: BBa_K525007
start: 56
end: 59
orientation: inline

K525515
type: SequenceAnnotation
persistent identity: annotation2161171
display id: annotation2161171
version: 1
top level: BBa_K525007
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K525007/annotation2161171/range2161171/1

https://synbiohub.org/public/igem/BBa_K525007/annotation2161171/range2161171/1
type: Range
persistent identity: range2161171
display id: range2161171
version: 1
direction: direction/forward
top level: BBa_K525007
start: 2407
end: 3999
orientation: inline

Freiburg Scar
type: SequenceAnnotation
persistent identity: annotation2161173
display id: annotation2161173
version: 1
top level: BBa_K525007
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K525007/annotation2161173/range2161173/1

https://synbiohub.org/public/igem/BBa_K525007/annotation2161173/range2161173/1
type: Range
persistent identity: range2161173
display id: range2161173
version: 1
direction: direction/forward
top level: BBa_K525007
start: 2719
end: 2724
orientation: inline

K525301
type: SequenceAnnotation
persistent identity: annotation2161168
display id: annotation2161168
version: 1
top level: BBa_K525007
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K525007/annotation2161168/range2161168/1

https://synbiohub.org/public/igem/BBa_K525007/annotation2161168/range2161168/1
type: Range
persistent identity: range2161168
display id: range2161168
version: 1
direction: direction/forward
top level: BBa_K525007
start: 73
end: 2400
orientation: inline

T7
type: SequenceAnnotation
persistent identity: annotation2161162
display id: annotation2161162
version: 1
top level: BBa_K525007
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K525007/annotation2161162/range2161162/1

https://synbiohub.org/public/igem/BBa_K525007/annotation2161162/range2161162/1
type: Range
persistent identity: range2161162
display id: range2161162
version: 1
direction: direction/forward
top level: BBa_K525007
start: 1
end: 20
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation2161164
display id: annotation2161164
version: 1
top level: BBa_K525007
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K525007/annotation2161164/range2161164/1

https://synbiohub.org/public/igem/BBa_K525007/annotation2161164/range2161164/1
type: Range
persistent identity: range2161164
display id: range2161164
version: 1
direction: direction/forward
top level: BBa_K525007
start: 53
end: 64
orientation: inline

lac operator
type: SequenceAnnotation
persistent identity: annotation2161163
display id: annotation2161163
version: 1
top level: BBa_K525007
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K525007/annotation2161163/range2161163/1

https://synbiohub.org/public/igem/BBa_K525007/annotation2161163/range2161163/1
type: Range
persistent identity: range2161163
display id: range2161163
version: 1
direction: direction/forward
top level: BBa_K525007
start: 20
end: 44
orientation: inline

Freiburg Scar
type: SequenceAnnotation
persistent identity: annotation2161170
display id: annotation2161170
version: 1
top level: BBa_K525007
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K525007/annotation2161170/range2161170/1

https://synbiohub.org/public/igem/BBa_K525007/annotation2161170/range2161170/1
type: Range
persistent identity: range2161170
display id: range2161170
version: 1
direction: direction/forward
top level: BBa_K525007
start: 2401
end: 2406
orientation: inline

SgsE domain
type: SequenceAnnotation
persistent identity: annotation2161167
display id: annotation2161167
version: 1
top level: BBa_K525007
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K525007/annotation2161167/range2161167/1

https://synbiohub.org/public/igem/BBa_K525007/annotation2161167/range2161167/1
type: Range
persistent identity: range2161167
display id: range2161167
version: 1
direction: direction/forward
top level: BBa_K525007
start: 73
end: 2400
orientation: inline

NgoMIV
type: SequenceAnnotation
persistent identity: annotation2161169
display id: annotation2161169
version: 1
top level: BBa_K525007
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K525007/annotation2161169/range2161169/1

https://synbiohub.org/public/igem/BBa_K525007/annotation2161169/range2161169/1
type: Range
persistent identity: range2161169
display id: range2161169
version: 1
direction: direction/forward
top level: BBa_K525007
start: 76
end: 82
orientation: inline

BisdA
type: SequenceAnnotation
persistent identity: annotation2161172
display id: annotation2161172
version: 1
top level: BBa_K525007
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K525007/annotation2161172/range2161172/1

https://synbiohub.org/public/igem/BBa_K525007/annotation2161172/range2161172/1
type: Range
persistent identity: range2161172
display id: range2161172
version: 1
direction: direction/forward
top level: BBa_K525007
start: 2407
end: 2718
orientation: inline

SgsE | Luc
type: ComponentDefinition
persistent identity: BBa K525311
display id: BBa_K525311
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Fusion Protein of S-Layer SgsE and Firefly-Luciferase
creator: Anna Drong
created: 2011-09-12T11:00:00Z
modified: 2015-05-08T01:12:34Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _690_
m user id: 0
owner id: 8543
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: PT7 + RBS + SgsE || luc
mutable notes: Assembled
mutable provenance: BBa_K525303; BBa_K525999
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: NgoMIV
sequence annotation: SgsE domain
sequence annotation: AgeI
sequence annotation: RBS
sequence annotation: ATG
sequence annotation: K525301
sequence annotation: pT7
sequence annotation: Freiburg scar
sequence annotation: K525999
sequence annotation: lac opterator
sequence annotation: TAA
sequence annotation: B0034
sequence annotation: Luciferase
sequence: https://synbiohub.org/public/igem/BBa_K525311_sequence/1

NgoMIV
type: SequenceAnnotation
persistent identity: annotation2137365
display id: annotation2137365
version: 1
top level: SgsE | Luc
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K525311/annotation2137365/range2137365/1

https://synbiohub.org/public/igem/BBa_K525311/annotation2137365/range2137365/1
type: Range
persistent identity: range2137365
display id: range2137365
version: 1
direction: direction/forward
top level: SgsE | Luc
start: 76
end: 81
orientation: inline

SgsE domain
type: SequenceAnnotation
persistent identity: annotation2137354
display id: annotation2137354
version: 1
top level: SgsE | Luc
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K525311/annotation2137354/range2137354/1

https://synbiohub.org/public/igem/BBa_K525311/annotation2137354/range2137354/1
type: Range
persistent identity: range2137354
display id: range2137354
version: 1
direction: direction/forward
top level: SgsE | Luc
start: 73
end: 2400
orientation: inline

AgeI
type: SequenceAnnotation
persistent identity: annotation2137369
display id: annotation2137369
version: 1
top level: SgsE | Luc
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K525311/annotation2137369/range2137369/1

https://synbiohub.org/public/igem/BBa_K525311/annotation2137369/range2137369/1
type: Range
persistent identity: range2137369
display id: range2137369
version: 1
direction: direction/forward
top level: SgsE | Luc
start: 4054
end: 4059
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2137353
display id: annotation2137353
version: 1
top level: SgsE | Luc
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K525311/annotation2137353/range2137353/1

https://synbiohub.org/public/igem/BBa_K525311/annotation2137353/range2137353/1
type: Range
persistent identity: range2137353
display id: range2137353
version: 1
direction: direction/forward
top level: SgsE | Luc
start: 56
end: 59
orientation: inline

ATG
type: SequenceAnnotation
persistent identity: annotation2137364
display id: annotation2137364
version: 1
top level: SgsE | Luc
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K525311/annotation2137364/range2137364/1

https://synbiohub.org/public/igem/BBa_K525311/annotation2137364/range2137364/1
type: Range
persistent identity: range2137364
display id: range2137364
version: 1
direction: direction/forward
top level: SgsE | Luc
start: 73
end: 75
orientation: inline

K525301
type: SequenceAnnotation
persistent identity: annotation2137362
display id: annotation2137362
version: 1
top level: SgsE | Luc
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K525311/annotation2137362/range2137362/1

https://synbiohub.org/public/igem/BBa_K525311/annotation2137362/range2137362/1
type: Range
persistent identity: range2137362
display id: range2137362
version: 1
direction: direction/forward
top level: SgsE | Luc
start: 73
end: 2400
orientation: inline

pT7
type: SequenceAnnotation
persistent identity: annotation2137343
display id: annotation2137343
version: 1
top level: SgsE | Luc
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K525311/annotation2137343/range2137343/1

https://synbiohub.org/public/igem/BBa_K525311/annotation2137343/range2137343/1
type: Range
persistent identity: range2137343
display id: range2137343
version: 1
direction: direction/forward
top level: SgsE | Luc
start: 1
end: 20
orientation: inline

Freiburg scar
type: SequenceAnnotation
persistent identity: annotation2137363
display id: annotation2137363
version: 1
top level: SgsE | Luc
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K525311/annotation2137363/range2137363/1

https://synbiohub.org/public/igem/BBa_K525311/annotation2137363/range2137363/1
type: Range
persistent identity: range2137363
display id: range2137363
version: 1
direction: direction/forward
top level: SgsE | Luc
start: 2401
end: 2406
orientation: inline

K525999
type: SequenceAnnotation
persistent identity: annotation2137367
display id: annotation2137367
version: 1
top level: SgsE | Luc
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K525311/annotation2137367/range2137367/1

https://synbiohub.org/public/igem/BBa_K525311/annotation2137367/range2137367/1
type: Range
persistent identity: range2137367
display id: range2137367
version: 1
direction: direction/forward
top level: SgsE | Luc
start: 2407
end: 4062
orientation: inline

lac opterator
type: SequenceAnnotation
persistent identity: annotation2137351
display id: annotation2137351
version: 1
top level: SgsE | Luc
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K525311/annotation2137351/range2137351/1

https://synbiohub.org/public/igem/BBa_K525311/annotation2137351/range2137351/1
type: Range
persistent identity: range2137351
display id: range2137351
version: 1
direction: direction/forward
top level: SgsE | Luc
start: 20
end: 44
orientation: inline

TAA
type: SequenceAnnotation
persistent identity: annotation2137368
display id: annotation2137368
version: 1
top level: SgsE | Luc
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K525311/annotation2137368/range2137368/1

https://synbiohub.org/public/igem/BBa_K525311/annotation2137368/range2137368/1
type: Range
persistent identity: range2137368
display id: range2137368
version: 1
direction: direction/forward
top level: SgsE | Luc
start: 4060
end: 4062
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation2137352
display id: annotation2137352
version: 1
top level: SgsE | Luc
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K525311/annotation2137352/range2137352/1

https://synbiohub.org/public/igem/BBa_K525311/annotation2137352/range2137352/1
type: Range
persistent identity: range2137352
display id: range2137352
version: 1
direction: direction/forward
top level: SgsE | Luc
start: 53
end: 64
orientation: inline

Luciferase
type: SequenceAnnotation
persistent identity: annotation2137366
display id: annotation2137366
version: 1
top level: SgsE | Luc
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K525311/annotation2137366/range2137366/1

https://synbiohub.org/public/igem/BBa_K525311/annotation2137366/range2137366/1
type: Range
persistent identity: range2137366
display id: range2137366
version: 1
direction: direction/forward
top level: SgsE | Luc
start: 2407
end: 4062
orientation: inline

SP-Lipocal
type: ComponentDefinition
persistent identity: BBa K157039
display id: BBa_K157039
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: SP-Lipocalin-GGGSlinker-Tm-Splitluciferase1/2
creator: iGEM Team Freiburg 2008
created: 2008-10-25T11:00:00Z
modified: 2015-05-08T01:10:55Z
discontinued: false
dominant: false
experience: experience/None
group u list: _232_
m user id: 0
owner id: 1673
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: A composite part consisting of the signal peptide of Erbb1/EGF-receptor, scFV-antiNIP, GGGSflexlinker (extracellular part), the trans-membrane region of the Erbb1/EGF-receptor and the C-terminal part of the split fluorophor YFP with a fluolinker joined to its N-Terminus (intracellular part).
mutable notes: none
mutable provenance: Derived by cloning of the parts BBa_K157001, BBa_K157004, BBa_K157013, BBa_K157002 and the second half of split Luciferase For further information see the description of the parts mentioned above.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: NgoMI
sequence annotation: Lipocalin
sequence annotation: Scar (Thr, Gly)
sequence annotation: Tm
sequence annotation: AgeI
sequence annotation: Splitluciferase1/2
sequence annotation: SP-Lipocalin-GGGSlinker-Tm-Splitluciferase1/2
sequence annotation: SP
sequence annotation: GGGS-Linker
sequence: https://synbiohub.org/public/igem/BBa_K157039_sequence/1

NgoMI
type: SequenceAnnotation
persistent identity: annotation1990303
display id: annotation1990303
version: 1
top level: SP-Lipocal
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157039/annotation1990303/range1990303/1

https://synbiohub.org/public/igem/BBa_K157039/annotation1990303/range1990303/1
type: Range
persistent identity: range1990303
display id: range1990303
version: 1
direction: direction/forward
top level: SP-Lipocal
start: 4
end: 9
orientation: inline

Lipocalin
type: SequenceAnnotation
persistent identity: annotation1993429
display id: annotation1993429
version: 1
top level: SP-Lipocal
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157039/annotation1993429/range1993429/1

https://synbiohub.org/public/igem/BBa_K157039/annotation1993429/range1993429/1
type: Range
persistent identity: range1993429
display id: range1993429
version: 1
direction: direction/forward
top level: SP-Lipocal
start: 88
end: 609
orientation: inline

Scar (Thr, Gly)
type: SequenceAnnotation
persistent identity: annotation1993430
display id: annotation1993430
version: 1
top level: SP-Lipocal
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157039/annotation1993430/range1993430/1

https://synbiohub.org/public/igem/BBa_K157039/annotation1993430/range1993430/1
type: Range
persistent identity: range1993430
display id: range1993430
version: 1
direction: direction/forward
top level: SP-Lipocal
start: 610
end: 615
orientation: inline

Tm
type: SequenceAnnotation
persistent identity: annotation1993433
display id: annotation1993433
version: 1
top level: SP-Lipocal
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157039/annotation1993433/range1993433/1

https://synbiohub.org/public/igem/BBa_K157039/annotation1993433/range1993433/1
type: Range
persistent identity: range1993433
display id: range1993433
version: 1
direction: direction/forward
top level: SP-Lipocal
start: 667
end: 735
orientation: inline

AgeI
type: SequenceAnnotation
persistent identity: annotation1990304
display id: annotation1990304
version: 1
top level: SP-Lipocal
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157039/annotation1990304/range1990304/1

https://synbiohub.org/public/igem/BBa_K157039/annotation1990304/range1990304/1
type: Range
persistent identity: range1990304
display id: range1990304
version: 1
direction: direction/forward
top level: SP-Lipocal
start: 970
end: 975
orientation: inline

Splitluciferase1/2
type: SequenceAnnotation
persistent identity: annotation1993435
display id: annotation1993435
version: 1
top level: SP-Lipocal
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157039/annotation1993435/range1993435/1

https://synbiohub.org/public/igem/BBa_K157039/annotation1993435/range1993435/1
type: Range
persistent identity: range1993435
display id: range1993435
version: 1
direction: direction/forward
top level: SP-Lipocal
start: 742
end: 969
orientation: inline

Scar (Thr, Gly)
type: SequenceAnnotation
persistent identity: annotation1993434
display id: annotation1993434
version: 1
top level: SP-Lipocal
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157039/annotation1993434/range1993434/1

https://synbiohub.org/public/igem/BBa_K157039/annotation1993434/range1993434/1
type: Range
persistent identity: range1993434
display id: range1993434
version: 1
direction: direction/forward
top level: SP-Lipocal
start: 736
end: 741
orientation: inline

SP-Lipocalin-GGGSlinker-Tm-Splitluciferase1/2
type: SequenceAnnotation
persistent identity: annotation1990305
display id: annotation1990305
version: 1
top level: SP-Lipocal
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K157039/annotation1990305/range1990305/1

https://synbiohub.org/public/igem/BBa_K157039/annotation1990305/range1990305/1
type: Range
persistent identity: range1990305
display id: range1990305
version: 1
direction: direction/forward
top level: SP-Lipocal
start: 1
end: 975
orientation: inline

Scar (Thr, Gly)
type: SequenceAnnotation
persistent identity: annotation1993432
display id: annotation1993432
version: 1
top level: SP-Lipocal
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157039/annotation1993432/range1993432/1

https://synbiohub.org/public/igem/BBa_K157039/annotation1993432/range1993432/1
type: Range
persistent identity: range1993432
display id: range1993432
version: 1
direction: direction/forward
top level: SP-Lipocal
start: 661
end: 666
orientation: inline

SP
type: SequenceAnnotation
persistent identity: annotation1993427
display id: annotation1993427
version: 1
top level: SP-Lipocal
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157039/annotation1993427/range1993427/1

https://synbiohub.org/public/igem/BBa_K157039/annotation1993427/range1993427/1
type: Range
persistent identity: range1993427
display id: range1993427
version: 1
direction: direction/forward
top level: SP-Lipocal
start: 10
end: 81
orientation: inline

GGGS-Linker
type: SequenceAnnotation
persistent identity: annotation1993431
display id: annotation1993431
version: 1
top level: SP-Lipocal
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157039/annotation1993431/range1993431/1

https://synbiohub.org/public/igem/BBa_K157039/annotation1993431/range1993431/1
type: Range
persistent identity: range1993431
display id: range1993431
version: 1
direction: direction/forward
top level: SP-Lipocal
start: 616
end: 660
orientation: inline

Scar (Thr, Gly)
type: SequenceAnnotation
persistent identity: annotation1993428
display id: annotation1993428
version: 1
top level: SP-Lipocal
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157039/annotation1993428/range1993428/1

https://synbiohub.org/public/igem/BBa_K157039/annotation1993428/range1993428/1
type: Range
persistent identity: range1993428
display id: range1993428
version: 1
direction: direction/forward
top level: SP-Lipocal
start: 82
end: 87
orientation: inline

BBa_K157004
type: ComponentDefinition
persistent identity: BBa K157004
display id: BBa_K157004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Fluoresceine-A-binding
creator: iGEM Team Freiburg 2008
created: 2008-10-25T11:00:00Z
modified: 2015-05-08T01:10:54Z
discontinued: false
dominant: false
experience: experience/None
group u list: _232_
m user id: 0
owner id: 1673
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Fluoresceine A -binding derivative of a Lipocalin (BBP, bilin binding protein from pieris brassicae), originally designed by Arne Skerra [see Ref. 1,2].
mutable notes: Between BioBrick 1.0 sites this part is flanked 5' by NgoMI(=NgoMIV) and 3' AgeI(=PinAI) to facilitate in frame cloning for protein fusions.
mutable provenance: Gene synthesis by GeneArt, optimized for expression in homo sapiens.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: Lipocalin FluA
sequence: https://synbiohub.org/public/igem/BBa_K157004_sequence/1

Lipocalin FluA
type: SequenceAnnotation
persistent identity: annotation2040659
display id: annotation2040659
version: 1
top level: BBa_K157004
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K157004/annotation2040659/range2040659/1

https://synbiohub.org/public/igem/BBa_K157004/annotation2040659/range2040659/1
type: Range
persistent identity: range2040659
display id: range2040659
version: 1
direction: direction/forward
top level: BBa_K157004
start: 1
end: 522
orientation: inline

BBa_K525562
type: ComponentDefinition
persistent identity: BBa K525562
display id: BBa_K525562
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Fusion protein of NADP+ Oxidoreductase and BisdA and BisdB with middle strong promoter,RBS
creator: Anna Drong
created: 2011-10-27T11:00:00Z
modified: 2015-05-08T01:12:34Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _690_
m user id: 0
owner id: 8543
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Fusion protein of ferredoxin-NADP+ oxidoreductase, BisdA and BisdB; RFC 25 (Freiburg BioBrick assembly standard) for characterization of intra- and extracellular BPA degradation.
mutable notes: Assembled
mutable provenance: K525499; K12300; K123001
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: BisdA
sequence annotation: Freiburg Scar
sequence annotation: AgeI
sequence annotation: NgoMIV
sequence annotation: RBS
sequence annotation: K123000
sequence annotation: TAA
sequence annotation: BisdB
sequence annotation: Reductase
sequence annotation: K525499
sequence annotation: promoter
sequence annotation: B0034
sequence annotation: ATG
sequence annotation: K123001
sequence: https://synbiohub.org/public/igem/BBa_K525562_sequence/1

BisdA
type: SequenceAnnotation
persistent identity: annotation2161293
display id: annotation2161293
version: 1
top level: BBa_K525562
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K525562/annotation2161293/range2161293/1

https://synbiohub.org/public/igem/BBa_K525562/annotation2161293/range2161293/1
type: Range
persistent identity: range2161293
display id: range2161293
version: 1
direction: direction/forward
top level: BBa_K525562
start: 818
end: 1128
orientation: inline

Freiburg Scar
type: SequenceAnnotation
persistent identity: annotation2161292
display id: annotation2161292
version: 1
top level: BBa_K525562
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K525562/annotation2161292/range2161292/1

https://synbiohub.org/public/igem/BBa_K525562/annotation2161292/range2161292/1
type: Range
persistent identity: range2161292
display id: range2161292
version: 1
direction: direction/forward
top level: BBa_K525562
start: 812
end: 817
orientation: inline

AgeI
type: SequenceAnnotation
persistent identity: annotation2160993
display id: annotation2160993
version: 1
top level: BBa_K525562
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K525562/annotation2160993/range2160993/1

https://synbiohub.org/public/igem/BBa_K525562/annotation2160993/range2160993/1
type: Range
persistent identity: range2160993
display id: range2160993
version: 1
direction: direction/forward
top level: BBa_K525562
start: 2402
end: 2407
orientation: inline

NgoMIV
type: SequenceAnnotation
persistent identity: annotation2160995
display id: annotation2160995
version: 1
top level: BBa_K525562
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K525562/annotation2160995/range2160995/1

https://synbiohub.org/public/igem/BBa_K525562/annotation2160995/range2160995/1
type: Range
persistent identity: range2160995
display id: range2160995
version: 1
direction: direction/forward
top level: BBa_K525562
start: 65
end: 70
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2161296
display id: annotation2161296
version: 1
top level: BBa_K525562
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K525562/annotation2161296/range2161296/1

https://synbiohub.org/public/igem/BBa_K525562/annotation2161296/range2161296/1
type: Range
persistent identity: range2161296
display id: range2161296
version: 1
direction: direction/forward
top level: BBa_K525562
start: 47
end: 50
orientation: inline

K123000
type: SequenceAnnotation
persistent identity: annotation2161000
display id: annotation2161000
version: 1
top level: BBa_K525562
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K525562/annotation2161000/range2161000/1

https://synbiohub.org/public/igem/BBa_K525562/annotation2161000/range2161000/1
type: Range
persistent identity: range2161000
display id: range2161000
version: 1
direction: direction/forward
top level: BBa_K525562
start: 818
end: 1128
orientation: inline

TAA
type: SequenceAnnotation
persistent identity: annotation2160994
display id: annotation2160994
version: 1
top level: BBa_K525562
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K525562/annotation2160994/range2160994/1

https://synbiohub.org/public/igem/BBa_K525562/annotation2160994/range2160994/1
type: Range
persistent identity: range2160994
display id: range2160994
version: 1
direction: direction/forward
top level: BBa_K525562
start: 2408
end: 2410
orientation: inline

BisdB
type: SequenceAnnotation
persistent identity: annotation2161294
display id: annotation2161294
version: 1
top level: BBa_K525562
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K525562/annotation2161294/range2161294/1

https://synbiohub.org/public/igem/BBa_K525562/annotation2161294/range2161294/1
type: Range
persistent identity: range2161294
display id: range2161294
version: 1
direction: direction/forward
top level: BBa_K525562
start: 1135
end: 2410
orientation: inline

Reductase
type: SequenceAnnotation
persistent identity: annotation2161295
display id: annotation2161295
version: 1
top level: BBa_K525562
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K525562/annotation2161295/range2161295/1

https://synbiohub.org/public/igem/BBa_K525562/annotation2161295/range2161295/1
type: Range
persistent identity: range2161295
display id: range2161295
version: 1
direction: direction/forward
top level: BBa_K525562
start: 162
end: 811
orientation: inline

K525499
type: SequenceAnnotation
persistent identity: annotation2160999
display id: annotation2160999
version: 1
top level: BBa_K525562
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K525562/annotation2160999/range2160999/1

https://synbiohub.org/public/igem/BBa_K525562/annotation2160999/range2160999/1
type: Range
persistent identity: range2160999
display id: range2160999
version: 1
direction: direction/forward
top level: BBa_K525562
start: 62
end: 811
orientation: inline

promoter
type: SequenceAnnotation
persistent identity: annotation2160997
display id: annotation2160997
version: 1
top level: BBa_K525562
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K525562/annotation2160997/range2160997/1

https://synbiohub.org/public/igem/BBa_K525562/annotation2160997/range2160997/1
type: Range
persistent identity: range2160997
display id: range2160997
version: 1
direction: direction/forward
top level: BBa_K525562
start: 1
end: 35
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation2160998
display id: annotation2160998
version: 1
top level: BBa_K525562
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K525562/annotation2160998/range2160998/1

https://synbiohub.org/public/igem/BBa_K525562/annotation2160998/range2160998/1
type: Range
persistent identity: range2160998
display id: range2160998
version: 1
direction: direction/forward
top level: BBa_K525562
start: 44
end: 55
orientation: inline

ATG
type: SequenceAnnotation
persistent identity: annotation2160996
display id: annotation2160996
version: 1
top level: BBa_K525562
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K525562/annotation2160996/range2160996/1

https://synbiohub.org/public/igem/BBa_K525562/annotation2160996/range2160996/1
type: Range
persistent identity: range2160996
display id: range2160996
version: 1
direction: direction/forward
top level: BBa_K525562
start: 62
end: 64
orientation: inline

K123001
type: SequenceAnnotation
persistent identity: annotation2161001
display id: annotation2161001
version: 1
top level: BBa_K525562
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K525562/annotation2161001/range2161001/1

https://synbiohub.org/public/igem/BBa_K525562/annotation2161001/range2161001/1
type: Range
persistent identity: range2161001
display id: range2161001
version: 1
direction: direction/forward
top level: BBa_K525562
start: 1135
end: 2410
orientation: inline

Freiburg Scar
type: SequenceAnnotation
persistent identity: annotation2161138
display id: annotation2161138
version: 1
top level: BBa_K525562
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K525562/annotation2161138/range2161138/1

https://synbiohub.org/public/igem/BBa_K525562/annotation2161138/range2161138/1
type: Range
persistent identity: range2161138
display id: range2161138
version: 1
direction: direction/forward
top level: BBa_K525562
start: 1129
end: 1134
orientation: inline

BisdAB
type: ComponentDefinition
persistent identity: BBa K525517
display id: BBa_K525517
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Fusion protein of BisdA and BisdB behind constitutive promoter
creator: Jonas Aretz
created: 2011-09-13T11:00:00Z
modified: 2015-05-08T01:12:34Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _690_
m user id: 0
owner id: 6670
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Fusion protein of BisdA and BisdB behind constitutive promoter
mutable notes: Fusion protein of BisdA and BisdB behind constitutive promoter
mutable provenance: Assembled
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_B0034
component: BBa_J23110
component: BBa_K525515
sequence annotation: BBa_B0034
sequence annotation: BBa_K525515
sequence annotation: BBa_J23110
sequence: https://synbiohub.org/public/igem/BBa_K525517_sequence/1

BBa_B0034
type: Component
persistent identity: component2128148
display id: component2128148
version: 1
top level: BisdAB
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_J23110
type: Component
persistent identity: component2128146
display id: component2128146
version: 1
top level: BisdAB
access: public
definition: https://synbiohub.org/public/igem/BBa_J23110/1

BBa_K525515
type: Component
persistent identity: component2128151
display id: component2128151
version: 1
top level: BisdAB
access: public
definition: BisdAB

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2128148
display id: annotation2128148
version: 1
top level: BisdAB
location: https://synbiohub.org/public/igem/BBa_K525517/annotation2128148/range2128148/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K525517/annotation2128148/range2128148/1
type: Range
persistent identity: range2128148
display id: range2128148
version: 1
direction: direction/reverse
top level: BisdAB
start: 44
end: 55
orientation: inline

BBa_K525515
type: SequenceAnnotation
persistent identity: annotation2128151
display id: annotation2128151
version: 1
top level: BisdAB
location: https://synbiohub.org/public/igem/BBa_K525517/annotation2128151/range2128151/1
component: BBa_K525515

https://synbiohub.org/public/igem/BBa_K525517/annotation2128151/range2128151/1
type: Range
persistent identity: range2128151
display id: range2128151
version: 1
direction: direction/reverse
top level: BisdAB
start: 62
end: 1663
orientation: inline

BBa_J23110
type: SequenceAnnotation
persistent identity: annotation2128146
display id: annotation2128146
version: 1
top level: BisdAB
location: https://synbiohub.org/public/igem/BBa_K525517/annotation2128146/range2128146/1
component: BBa_J23110

https://synbiohub.org/public/igem/BBa_K525517/annotation2128146/range2128146/1
type: Range
persistent identity: range2128146
display id: range2128146
version: 1
direction: direction/reverse
top level: BisdAB
start: 1
end: 35
orientation: inline

SP-AntiNIP
type: ComponentDefinition
persistent identity: BBa K157024
display id: BBa_K157024
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: SP-AntiNIP-GGGSlinker-Tm-betaLactamase(Bla1)
creator: iGEM Team Freiburg 2008
created: 2008-10-25T11:00:00Z
modified: 2015-05-08T01:10:55Z
discontinued: false
dominant: false
experience: experience/None
group u list: _232_
m user id: 0
owner id: 1673
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: A composite part consisting of the signal-peptide of Erbb1/EGF-receptor, scFV-antiNIP, GGGSflexlinker (extracellular part), the trans-membrane region of the Erbb1/EGF-receptor and the N-terminal part of split-enzyme beta-Lactamase (intracellular part).
mutable notes: none
mutable provenance: Derived by cloning of the parts BBa_K157001, BBa_K157003, BBa_K157013, BBa_K157002 and BBa_I757011. For further information see the description of the parts mentioned above.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: Scar (Thr, Gly)
sequence annotation: SP-AntiNIP-GGGSlinker-Tm-betaLactamase(Bla1)
sequence annotation: NgoMI
sequence annotation: Bla1
sequence annotation: Tm
sequence annotation: SP
sequence annotation: GGGS-Linker
sequence annotation: Sc-Fv_anti-NIP
sequence annotation: AgeI
sequence: https://synbiohub.org/public/igem/BBa_K157024_sequence/1

Scar (Thr, Gly)
type: SequenceAnnotation
persistent identity: annotation1992603
display id: annotation1992603
version: 1
top level: SP-AntiNIP
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157024/annotation1992603/range1992603/1

https://synbiohub.org/public/igem/BBa_K157024/annotation1992603/range1992603/1
type: Range
persistent identity: range1992603
display id: range1992603
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 82
end: 87
orientation: inline

SP-AntiNIP-GGGSlinker-Tm-betaLactamase(Bla1)
type: SequenceAnnotation
persistent identity: annotation1990168
display id: annotation1990168
version: 1
top level: SP-AntiNIP
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K157024/annotation1990168/range1990168/1

https://synbiohub.org/public/igem/BBa_K157024/annotation1990168/range1990168/1
type: Range
persistent identity: range1990168
display id: range1990168
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 1
end: 1485
orientation: inline

NgoMI
type: SequenceAnnotation
persistent identity: annotation1990166
display id: annotation1990166
version: 1
top level: SP-AntiNIP
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157024/annotation1990166/range1990166/1

https://synbiohub.org/public/igem/BBa_K157024/annotation1990166/range1990166/1
type: Range
persistent identity: range1990166
display id: range1990166
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 4
end: 9
orientation: inline

Scar (Thr, Gly)
type: SequenceAnnotation
persistent identity: annotation1992606
display id: annotation1992606
version: 1
top level: SP-AntiNIP
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157024/annotation1992606/range1992606/1

https://synbiohub.org/public/igem/BBa_K157024/annotation1992606/range1992606/1
type: Range
persistent identity: range1992606
display id: range1992606
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 823
end: 828
orientation: inline

Bla1
type: SequenceAnnotation
persistent identity: annotation1992613
display id: annotation1992613
version: 1
top level: SP-AntiNIP
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157024/annotation1992613/range1992613/1

https://synbiohub.org/public/igem/BBa_K157024/annotation1992613/range1992613/1
type: Range
persistent identity: range1992613
display id: range1992613
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 955
end: 1479
orientation: inline

Scar (Thr, Gly)
type: SequenceAnnotation
persistent identity: annotation1992610
display id: annotation1992610
version: 1
top level: SP-AntiNIP
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157024/annotation1992610/range1992610/1

https://synbiohub.org/public/igem/BBa_K157024/annotation1992610/range1992610/1
type: Range
persistent identity: range1992610
display id: range1992610
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 874
end: 879
orientation: inline

Scar (Thr, Gly)
type: SequenceAnnotation
persistent identity: annotation1992612
display id: annotation1992612
version: 1
top level: SP-AntiNIP
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157024/annotation1992612/range1992612/1

https://synbiohub.org/public/igem/BBa_K157024/annotation1992612/range1992612/1
type: Range
persistent identity: range1992612
display id: range1992612
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 949
end: 954
orientation: inline

Tm
type: SequenceAnnotation
persistent identity: annotation1992611
display id: annotation1992611
version: 1
top level: SP-AntiNIP
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157024/annotation1992611/range1992611/1

https://synbiohub.org/public/igem/BBa_K157024/annotation1992611/range1992611/1
type: Range
persistent identity: range1992611
display id: range1992611
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 880
end: 948
orientation: inline

SP
type: SequenceAnnotation
persistent identity: annotation1992602
display id: annotation1992602
version: 1
top level: SP-AntiNIP
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157024/annotation1992602/range1992602/1

https://synbiohub.org/public/igem/BBa_K157024/annotation1992602/range1992602/1
type: Range
persistent identity: range1992602
display id: range1992602
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 10
end: 81
orientation: inline

GGGS-Linker
type: SequenceAnnotation
persistent identity: annotation1992608
display id: annotation1992608
version: 1
top level: SP-AntiNIP
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157024/annotation1992608/range1992608/1

https://synbiohub.org/public/igem/BBa_K157024/annotation1992608/range1992608/1
type: Range
persistent identity: range1992608
display id: range1992608
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 829
end: 873
orientation: inline

Sc-Fv_anti-NIP
type: SequenceAnnotation
persistent identity: annotation1992604
display id: annotation1992604
version: 1
top level: SP-AntiNIP
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157024/annotation1992604/range1992604/1

https://synbiohub.org/public/igem/BBa_K157024/annotation1992604/range1992604/1
type: Range
persistent identity: range1992604
display id: range1992604
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 88
end: 822
orientation: inline

AgeI
type: SequenceAnnotation
persistent identity: annotation1990167
display id: annotation1990167
version: 1
top level: SP-AntiNIP
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157024/annotation1990167/range1990167/1

https://synbiohub.org/public/igem/BBa_K157024/annotation1990167/range1990167/1
type: Range
persistent identity: range1990167
display id: range1990167
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 1480
end: 1485
orientation: inline

SgsE
type: ComponentDefinition
persistent identity: BBa K525303
display id: BBa_K525303
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: S-Layer SgsE from Geobacillus stearothermophilus with T7/lac
creator: Timo Wolf
created: 2011-09-09T11:00:00Z
modified: 2015-05-08T01:12:34Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _690_
m user id: 0
owner id: 6668
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: S-Layer SgsE from Geobacillus stearothermophilus
mutable notes: Synthesis
mutable provenance: Synthesis
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: B0034
sequence annotation: T7 promoter
sequence annotation: NgoMIV
sequence annotation: K525301
sequence annotation: ATG
sequence annotation: AgeI
sequence annotation: TAA
sequence annotation: RBS
sequence annotation: lac operator
sequence annotation: SgsE domain
sequence: https://synbiohub.org/public/igem/BBa_K525303_sequence/1

B0034
type: SequenceAnnotation
persistent identity: annotation2128152
display id: annotation2128152
version: 1
top level: SgsE
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K525303/annotation2128152/range2128152/1

https://synbiohub.org/public/igem/BBa_K525303/annotation2128152/range2128152/1
type: Range
persistent identity: range2128152
display id: range2128152
version: 1
direction: direction/forward
top level: SgsE
start: 53
end: 64
orientation: inline

T7 promoter
type: SequenceAnnotation
persistent identity: annotation2128160
display id: annotation2128160
version: 1
top level: SgsE
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K525303/annotation2128160/range2128160/1

https://synbiohub.org/public/igem/BBa_K525303/annotation2128160/range2128160/1
type: Range
persistent identity: range2128160
display id: range2128160
version: 1
direction: direction/forward
top level: SgsE
start: 1
end: 20
orientation: inline

NgoMIV
type: SequenceAnnotation
persistent identity: annotation2129998
display id: annotation2129998
version: 1
top level: SgsE
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K525303/annotation2129998/range2129998/1

https://synbiohub.org/public/igem/BBa_K525303/annotation2129998/range2129998/1
type: Range
persistent identity: range2129998
display id: range2129998
version: 1
direction: direction/forward
top level: SgsE
start: 76
end: 81
orientation: inline

K525301
type: SequenceAnnotation
persistent identity: annotation2128153
display id: annotation2128153
version: 1
top level: SgsE
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K525303/annotation2128153/range2128153/1

https://synbiohub.org/public/igem/BBa_K525303/annotation2128153/range2128153/1
type: Range
persistent identity: range2128153
display id: range2128153
version: 1
direction: direction/forward
top level: SgsE
start: 73
end: 2409
orientation: inline

ATG
type: SequenceAnnotation
persistent identity: annotation2129997
display id: annotation2129997
version: 1
top level: SgsE
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K525303/annotation2129997/range2129997/1

https://synbiohub.org/public/igem/BBa_K525303/annotation2129997/range2129997/1
type: Range
persistent identity: range2129997
display id: range2129997
version: 1
direction: direction/forward
top level: SgsE
start: 73
end: 75
orientation: inline

AgeI
type: SequenceAnnotation
persistent identity: annotation2130000
display id: annotation2130000
version: 1
top level: SgsE
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K525303/annotation2130000/range2130000/1

https://synbiohub.org/public/igem/BBa_K525303/annotation2130000/range2130000/1
type: Range
persistent identity: range2130000
display id: range2130000
version: 1
direction: direction/forward
top level: SgsE
start: 2401
end: 2406
orientation: inline

TAA
type: SequenceAnnotation
persistent identity: annotation2129999
display id: annotation2129999
version: 1
top level: SgsE
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K525303/annotation2129999/range2129999/1

https://synbiohub.org/public/igem/BBa_K525303/annotation2129999/range2129999/1
type: Range
persistent identity: range2129999
display id: range2129999
version: 1
direction: direction/forward
top level: SgsE
start: 2407
end: 2409
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2129995
display id: annotation2129995
version: 1
top level: SgsE
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K525303/annotation2129995/range2129995/1

https://synbiohub.org/public/igem/BBa_K525303/annotation2129995/range2129995/1
type: Range
persistent identity: range2129995
display id: range2129995
version: 1
direction: direction/forward
top level: SgsE
start: 56
end: 59
orientation: inline

lac operator
type: SequenceAnnotation
persistent identity: annotation2129994
display id: annotation2129994
version: 1
top level: SgsE
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K525303/annotation2129994/range2129994/1

https://synbiohub.org/public/igem/BBa_K525303/annotation2129994/range2129994/1
type: Range
persistent identity: range2129994
display id: range2129994
version: 1
direction: direction/forward
top level: SgsE
start: 20
end: 44
orientation: inline

SgsE domain
type: SequenceAnnotation
persistent identity: annotation2129996
display id: annotation2129996
version: 1
top level: SgsE
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K525303/annotation2129996/range2129996/1

https://synbiohub.org/public/igem/BBa_K525303/annotation2129996/range2129996/1
type: Range
persistent identity: range2129996
display id: range2129996
version: 1
direction: direction/forward
top level: SgsE
start: 73
end: 2409
orientation: inline

cspB
type: ComponentDefinition
persistent identity: BBa K525221
display id: BBa_K525221
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: S-layer cspB from Corynebacterium halotolerans with TAT-Sequence and lipid anchor, PT7 and RBS
creator: Anna Drong
created: 2011-09-10T11:00:00Z
modified: 2015-05-08T01:12:34Z
discontinued: false
dominant: false
experience: experience/None
group u list: _690_
m user id: 0
owner id: 8543
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: pT7 + RBS + S-Layer + TAT-Sequenz + Lipidanker
mutable notes: PCR
mutable provenance: Corynebacterium halotolerans
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Promoter
sequence annotation: Lipid anchor
sequence annotation: S-layer
sequence annotation: TAT-Sequence
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_K525221_sequence/1

Promoter
type: SequenceAnnotation
persistent identity: annotation2128109
display id: annotation2128109
version: 1
top level: cspB
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K525221/annotation2128109/range2128109/1

https://synbiohub.org/public/igem/BBa_K525221/annotation2128109/range2128109/1
type: Range
persistent identity: range2128109
display id: range2128109
version: 1
direction: direction/forward
top level: cspB
start: 1
end: 22
orientation: inline

Lipid anchor
type: SequenceAnnotation
persistent identity: annotation2127315
display id: annotation2127315
version: 1
top level: cspB
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K525221/annotation2127315/range2127315/1

https://synbiohub.org/public/igem/BBa_K525221/annotation2127315/range2127315/1
type: Range
persistent identity: range2127315
display id: range2127315
version: 1
direction: direction/forward
top level: cspB
start: 1502
end: 1582
orientation: inline

S-layer
type: SequenceAnnotation
persistent identity: annotation2127392
display id: annotation2127392
version: 1
top level: cspB
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K525221/annotation2127392/range2127392/1

https://synbiohub.org/public/igem/BBa_K525221/annotation2127392/range2127392/1
type: Range
persistent identity: range2127392
display id: range2127392
version: 1
direction: direction/forward
top level: cspB
start: 124
end: 1501
orientation: inline

TAT-Sequence
type: SequenceAnnotation
persistent identity: annotation2127314
display id: annotation2127314
version: 1
top level: cspB
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K525221/annotation2127314/range2127314/1

https://synbiohub.org/public/igem/BBa_K525221/annotation2127314/range2127314/1
type: Range
persistent identity: range2127314
display id: range2127314
version: 1
direction: direction/forward
top level: cspB
start: 35
end: 123
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2127399
display id: annotation2127399
version: 1
top level: cspB
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K525221/annotation2127399/range2127399/1

https://synbiohub.org/public/igem/BBa_K525221/annotation2127399/range2127399/1
type: Range
persistent identity: range2127399
display id: range2127399
version: 1
direction: direction/forward
top level: cspB
start: 23
end: 34
orientation: inline

SgsE | YFP
type: ComponentDefinition
persistent identity: BBa K525305
display id: BBa_K525305
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Fusion Protein of S-Layer SgsE and mCitrine
creator: Timo Wolf
created: 2011-09-09T11:00:00Z
modified: 2016-01-26T01:30:31Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _690_
m user id: 4206
owner id: 6668
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: Fusion Protein of S-Layer SgsE and mCitrine
mutable notes: Fusion Protein of BBa_K525303 and BBa_J18931
mutable provenance: Fusion Protein of S-Layer SgsE and mCitrine
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: TAA
sequence annotation: lac operator
sequence annotation: K525301
sequence annotation: AgeI
sequence annotation: NgoMIV
sequence annotation: ATG
sequence annotation: J18931
sequence annotation: B0034
sequence annotation: mCitrine (YFP)
sequence annotation: Freiburg scar
sequence annotation: RBS
sequence annotation: T7 promoter
sequence annotation: SgsE domain
sequence: https://synbiohub.org/public/igem/BBa_K525305_sequence/1

TAA
type: SequenceAnnotation
persistent identity: annotation2127337
display id: annotation2127337
version: 1
top level: SgsE | YFP
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K525305/annotation2127337/range2127337/1

https://synbiohub.org/public/igem/BBa_K525305/annotation2127337/range2127337/1
type: Range
persistent identity: range2127337
display id: range2127337
version: 1
direction: direction/forward
top level: SgsE | YFP
start: 3127
end: 3129
orientation: inline

lac operator
type: SequenceAnnotation
persistent identity: annotation2127328
display id: annotation2127328
version: 1
top level: SgsE | YFP
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K525305/annotation2127328/range2127328/1

https://synbiohub.org/public/igem/BBa_K525305/annotation2127328/range2127328/1
type: Range
persistent identity: range2127328
display id: range2127328
version: 1
direction: direction/forward
top level: SgsE | YFP
start: 20
end: 44
orientation: inline

K525301
type: SequenceAnnotation
persistent identity: annotation2127339
display id: annotation2127339
version: 1
top level: SgsE | YFP
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K525305/annotation2127339/range2127339/1

https://synbiohub.org/public/igem/BBa_K525305/annotation2127339/range2127339/1
type: Range
persistent identity: range2127339
display id: range2127339
version: 1
direction: direction/forward
top level: SgsE | YFP
start: 73
end: 2400
orientation: inline

AgeI
type: SequenceAnnotation
persistent identity: annotation2127338
display id: annotation2127338
version: 1
top level: SgsE | YFP
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K525305/annotation2127338/range2127338/1

https://synbiohub.org/public/igem/BBa_K525305/annotation2127338/range2127338/1
type: Range
persistent identity: range2127338
display id: range2127338
version: 1
direction: direction/forward
top level: SgsE | YFP
start: 3121
end: 3126
orientation: inline

NgoMIV
type: SequenceAnnotation
persistent identity: annotation2127332
display id: annotation2127332
version: 1
top level: SgsE | YFP
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K525305/annotation2127332/range2127332/1

https://synbiohub.org/public/igem/BBa_K525305/annotation2127332/range2127332/1
type: Range
persistent identity: range2127332
display id: range2127332
version: 1
direction: direction/forward
top level: SgsE | YFP
start: 76
end: 81
orientation: inline

ATG
type: SequenceAnnotation
persistent identity: annotation2127333
display id: annotation2127333
version: 1
top level: SgsE | YFP
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K525305/annotation2127333/range2127333/1

https://synbiohub.org/public/igem/BBa_K525305/annotation2127333/range2127333/1
type: Range
persistent identity: range2127333
display id: range2127333
version: 1
direction: direction/forward
top level: SgsE | YFP
start: 73
end: 75
orientation: inline

J18931
type: SequenceAnnotation
persistent identity: annotation2127341
display id: annotation2127341
version: 1
top level: SgsE | YFP
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K525305/annotation2127341/range2127341/1

https://synbiohub.org/public/igem/BBa_K525305/annotation2127341/range2127341/1
type: Range
persistent identity: range2127341
display id: range2127341
version: 1
direction: direction/forward
top level: SgsE | YFP
start: 2407
end: 3129
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation2127331
display id: annotation2127331
version: 1
top level: SgsE | YFP
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K525305/annotation2127331/range2127331/1

https://synbiohub.org/public/igem/BBa_K525305/annotation2127331/range2127331/1
type: Range
persistent identity: range2127331
display id: range2127331
version: 1
direction: direction/forward
top level: SgsE | YFP
start: 53
end: 64
orientation: inline

mCitrine (YFP)
type: SequenceAnnotation
persistent identity: annotation2127336
display id: annotation2127336
version: 1
top level: SgsE | YFP
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K525305/annotation2127336/range2127336/1

https://synbiohub.org/public/igem/BBa_K525305/annotation2127336/range2127336/1
type: Range
persistent identity: range2127336
display id: range2127336
version: 1
direction: direction/forward
top level: SgsE | YFP
start: 2407
end: 3129
orientation: inline

Freiburg scar
type: SequenceAnnotation
persistent identity: annotation2127335
display id: annotation2127335
version: 1
top level: SgsE | YFP
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K525305/annotation2127335/range2127335/1

https://synbiohub.org/public/igem/BBa_K525305/annotation2127335/range2127335/1
type: Range
persistent identity: range2127335
display id: range2127335
version: 1
direction: direction/forward
top level: SgsE | YFP
start: 2401
end: 2406
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2127330
display id: annotation2127330
version: 1
top level: SgsE | YFP
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K525305/annotation2127330/range2127330/1

https://synbiohub.org/public/igem/BBa_K525305/annotation2127330/range2127330/1
type: Range
persistent identity: range2127330
display id: range2127330
version: 1
direction: direction/forward
top level: SgsE | YFP
start: 56
end: 59
orientation: inline

T7 promoter
type: SequenceAnnotation
persistent identity: annotation2127327
display id: annotation2127327
version: 1
top level: SgsE | YFP
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K525305/annotation2127327/range2127327/1

https://synbiohub.org/public/igem/BBa_K525305/annotation2127327/range2127327/1
type: Range
persistent identity: range2127327
display id: range2127327
version: 1
direction: direction/forward
top level: SgsE | YFP
start: 1
end: 20
orientation: inline

SgsE domain
type: SequenceAnnotation
persistent identity: annotation2127334
display id: annotation2127334
version: 1
top level: SgsE | YFP
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K525305/annotation2127334/range2127334/1

https://synbiohub.org/public/igem/BBa_K525305/annotation2127334/range2127334/1
type: Range
persistent identity: range2127334
display id: range2127334
version: 1
direction: direction/forward
top level: SgsE | YFP
start: 73
end: 2400
orientation: inline

SP-Lipocal
type: ComponentDefinition
persistent identity: BBa K157035
display id: BBa_K157035
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: SP-Lipocalin-GGGSlinker-Tm-nYFP
creator: iGEM Team Freiburg 2008
created: 2008-10-25T11:00:00Z
modified: 2015-05-08T01:10:55Z
discontinued: false
dominant: false
experience: experience/None
group u list: _232_
m user id: 0
owner id: 1673
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: A composite part consisting of the signal peptide of Erbb1/EGF-receptor, which ensures the transport to the cell membrane scFV-antiNIP, GGGSflexlinker (extracellular parts), the trans-membrane region of the Erbb1/EGF-receptor and the N-terminal part of the split fluorophor YFP(intracellular part).
mutable notes: none
mutable provenance: Derived by cloning of the parts BBa_K157001, BBa_K157004, BBa_K157013, BBa_K157002, BBa_K157008. For further information see the description of the parts mentioned above.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: Scar (Thr, Gly)
sequence annotation: GGGS-Linker
sequence annotation: SP
sequence annotation: NgoMI
sequence annotation: Lipocalin
sequence annotation: Tm
sequence annotation: BBa_K157035
sequence annotation: AgeI
sequence annotation: SP-Lipocalin-GGGSlinker-Tm-nYFP
sequence annotation: N-Term_YFP
sequence: https://synbiohub.org/public/igem/BBa_K157035_sequence/1

Scar (Thr, Gly)
type: SequenceAnnotation
persistent identity: annotation1993377
display id: annotation1993377
version: 1
top level: SP-Lipocal
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157035/annotation1993377/range1993377/1

https://synbiohub.org/public/igem/BBa_K157035/annotation1993377/range1993377/1
type: Range
persistent identity: range1993377
display id: range1993377
version: 1
direction: direction/forward
top level: SP-Lipocal
start: 661
end: 666
orientation: inline

GGGS-Linker
type: SequenceAnnotation
persistent identity: annotation1993376
display id: annotation1993376
version: 1
top level: SP-Lipocal
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157035/annotation1993376/range1993376/1

https://synbiohub.org/public/igem/BBa_K157035/annotation1993376/range1993376/1
type: Range
persistent identity: range1993376
display id: range1993376
version: 1
direction: direction/forward
top level: SP-Lipocal
start: 616
end: 660
orientation: inline

SP
type: SequenceAnnotation
persistent identity: annotation1993372
display id: annotation1993372
version: 1
top level: SP-Lipocal
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157035/annotation1993372/range1993372/1

https://synbiohub.org/public/igem/BBa_K157035/annotation1993372/range1993372/1
type: Range
persistent identity: range1993372
display id: range1993372
version: 1
direction: direction/forward
top level: SP-Lipocal
start: 10
end: 81
orientation: inline

NgoMI
type: SequenceAnnotation
persistent identity: annotation1993371
display id: annotation1993371
version: 1
top level: SP-Lipocal
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157035/annotation1993371/range1993371/1

https://synbiohub.org/public/igem/BBa_K157035/annotation1993371/range1993371/1
type: Range
persistent identity: range1993371
display id: range1993371
version: 1
direction: direction/forward
top level: SP-Lipocal
start: 4
end: 9
orientation: inline

Scar (Thr, Gly)
type: SequenceAnnotation
persistent identity: annotation1993379
display id: annotation1993379
version: 1
top level: SP-Lipocal
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157035/annotation1993379/range1993379/1

https://synbiohub.org/public/igem/BBa_K157035/annotation1993379/range1993379/1
type: Range
persistent identity: range1993379
display id: range1993379
version: 1
direction: direction/forward
top level: SP-Lipocal
start: 736
end: 741
orientation: inline

Lipocalin
type: SequenceAnnotation
persistent identity: annotation1993374
display id: annotation1993374
version: 1
top level: SP-Lipocal
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157035/annotation1993374/range1993374/1

https://synbiohub.org/public/igem/BBa_K157035/annotation1993374/range1993374/1
type: Range
persistent identity: range1993374
display id: range1993374
version: 1
direction: direction/forward
top level: SP-Lipocal
start: 88
end: 609
orientation: inline

Tm
type: SequenceAnnotation
persistent identity: annotation1993378
display id: annotation1993378
version: 1
top level: SP-Lipocal
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157035/annotation1993378/range1993378/1

https://synbiohub.org/public/igem/BBa_K157035/annotation1993378/range1993378/1
type: Range
persistent identity: range1993378
display id: range1993378
version: 1
direction: direction/forward
top level: SP-Lipocal
start: 667
end: 735
orientation: inline

Scar (Thr, Gly)
type: SequenceAnnotation
persistent identity: annotation1993373
display id: annotation1993373
version: 1
top level: SP-Lipocal
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157035/annotation1993373/range1993373/1

https://synbiohub.org/public/igem/BBa_K157035/annotation1993373/range1993373/1
type: Range
persistent identity: range1993373
display id: range1993373
version: 1
direction: direction/forward
top level: SP-Lipocal
start: 82
end: 87
orientation: inline

Scar (Thr, Gly)
type: SequenceAnnotation
persistent identity: annotation1993375
display id: annotation1993375
version: 1
top level: SP-Lipocal
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157035/annotation1993375/range1993375/1

https://synbiohub.org/public/igem/BBa_K157035/annotation1993375/range1993375/1
type: Range
persistent identity: range1993375
display id: range1993375
version: 1
direction: direction/forward
top level: SP-Lipocal
start: 610
end: 615
orientation: inline

BBa_K157035
type: SequenceAnnotation
persistent identity: annotation1993382
display id: annotation1993382
version: 1
top level: SP-Lipocal
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K157035/annotation1993382/range1993382/1

https://synbiohub.org/public/igem/BBa_K157035/annotation1993382/range1993382/1
type: Range
persistent identity: range1993382
display id: range1993382
version: 1
direction: direction/reverse
top level: SP-Lipocal
start: 1
end: 1218
orientation: inline

AgeI
type: SequenceAnnotation
persistent identity: annotation1993381
display id: annotation1993381
version: 1
top level: SP-Lipocal
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157035/annotation1993381/range1993381/1

https://synbiohub.org/public/igem/BBa_K157035/annotation1993381/range1993381/1
type: Range
persistent identity: range1993381
display id: range1993381
version: 1
direction: direction/forward
top level: SP-Lipocal
start: 1210
end: 1215
orientation: inline

SP-Lipocalin-GGGSlinker-Tm-nYFP
type: SequenceAnnotation
persistent identity: annotation1993370
display id: annotation1993370
version: 1
top level: SP-Lipocal
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K157035/annotation1993370/range1993370/1

https://synbiohub.org/public/igem/BBa_K157035/annotation1993370/range1993370/1
type: Range
persistent identity: range1993370
display id: range1993370
version: 1
direction: direction/forward
top level: SP-Lipocal
start: 1
end: 1215
orientation: inline

N-Term_YFP
type: SequenceAnnotation
persistent identity: annotation1993380
display id: annotation1993380
version: 1
top level: SP-Lipocal
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157035/annotation1993380/range1993380/1

https://synbiohub.org/public/igem/BBa_K157035/annotation1993380/range1993380/1
type: Range
persistent identity: range1993380
display id: range1993380
version: 1
direction: direction/forward
top level: SP-Lipocal
start: 742
end: 1209
orientation: inline

SP-AntiNIP
type: ComponentDefinition
persistent identity: BBa K157031
display id: BBa_K157031
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: SP-AntiNIP-GGGSlinker-Tm-Splitluciferase1/2
creator: iGEM Team Freiburg 2008
created: 2008-10-25T11:00:00Z
modified: 2015-05-08T01:10:55Z
discontinued: false
dominant: true
experience: experience/None
group u list: _232_
m user id: 0
owner id: 1673
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Part consists of the signal peptide of Erbb1/EGF-receptor, scFV-antiNIP, GGGSflexlinker (extracellular part), the trans-membrane region of the Erbb1/EGF-receptor and the C-terminal part of the split fluorophor YFP with a fluolinker joined to its N-Terminus (intracellular part).
mutable notes: none
mutable provenance: Derived by cloning of the parts BBa_K157001, BBa_K157003, BBa_K157013, BBa_K157002 and Part 2 of Split- Luciferase For further information see the description of the parts mentioned above.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: Scar (Thr, Gly)
sequence annotation: NgoMI
sequence annotation: AgeI
sequence annotation: SP-AntiNIP-GGGSlinker-Tm-Splitluciferase1/2
sequence annotation: Tm
sequence annotation: GGGS-Linker
sequence annotation: Splitluciferase1/2
sequence annotation: Sc-Fv_anti-NIP
sequence annotation: SP
sequence: https://synbiohub.org/public/igem/BBa_K157031_sequence/1

Scar (Thr, Gly)
type: SequenceAnnotation
persistent identity: annotation1993325
display id: annotation1993325
version: 1
top level: SP-AntiNIP
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157031/annotation1993325/range1993325/1

https://synbiohub.org/public/igem/BBa_K157031/annotation1993325/range1993325/1
type: Range
persistent identity: range1993325
display id: range1993325
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 949
end: 954
orientation: inline

NgoMI
type: SequenceAnnotation
persistent identity: annotation1990266
display id: annotation1990266
version: 1
top level: SP-AntiNIP
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157031/annotation1990266/range1990266/1

https://synbiohub.org/public/igem/BBa_K157031/annotation1990266/range1990266/1
type: Range
persistent identity: range1990266
display id: range1990266
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 4
end: 9
orientation: inline

AgeI
type: SequenceAnnotation
persistent identity: annotation1990267
display id: annotation1990267
version: 1
top level: SP-AntiNIP
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157031/annotation1990267/range1990267/1

https://synbiohub.org/public/igem/BBa_K157031/annotation1990267/range1990267/1
type: Range
persistent identity: range1990267
display id: range1990267
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 1231
end: 1236
orientation: inline

SP-AntiNIP-GGGSlinker-Tm-Splitluciferase1/2
type: SequenceAnnotation
persistent identity: annotation1990268
display id: annotation1990268
version: 1
top level: SP-AntiNIP
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K157031/annotation1990268/range1990268/1

https://synbiohub.org/public/igem/BBa_K157031/annotation1990268/range1990268/1
type: Range
persistent identity: range1990268
display id: range1990268
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 1
end: 1236
orientation: inline

Scar (Thr, Gly)
type: SequenceAnnotation
persistent identity: annotation1993321
display id: annotation1993321
version: 1
top level: SP-AntiNIP
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157031/annotation1993321/range1993321/1

https://synbiohub.org/public/igem/BBa_K157031/annotation1993321/range1993321/1
type: Range
persistent identity: range1993321
display id: range1993321
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 823
end: 828
orientation: inline

Tm
type: SequenceAnnotation
persistent identity: annotation1993324
display id: annotation1993324
version: 1
top level: SP-AntiNIP
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157031/annotation1993324/range1993324/1

https://synbiohub.org/public/igem/BBa_K157031/annotation1993324/range1993324/1
type: Range
persistent identity: range1993324
display id: range1993324
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 880
end: 948
orientation: inline

GGGS-Linker
type: SequenceAnnotation
persistent identity: annotation1993322
display id: annotation1993322
version: 1
top level: SP-AntiNIP
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157031/annotation1993322/range1993322/1

https://synbiohub.org/public/igem/BBa_K157031/annotation1993322/range1993322/1
type: Range
persistent identity: range1993322
display id: range1993322
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 829
end: 873
orientation: inline

Scar (Thr, Gly)
type: SequenceAnnotation
persistent identity: annotation1993323
display id: annotation1993323
version: 1
top level: SP-AntiNIP
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157031/annotation1993323/range1993323/1

https://synbiohub.org/public/igem/BBa_K157031/annotation1993323/range1993323/1
type: Range
persistent identity: range1993323
display id: range1993323
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 874
end: 879
orientation: inline

Splitluciferase1/2
type: SequenceAnnotation
persistent identity: annotation1993326
display id: annotation1993326
version: 1
top level: SP-AntiNIP
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157031/annotation1993326/range1993326/1

https://synbiohub.org/public/igem/BBa_K157031/annotation1993326/range1993326/1
type: Range
persistent identity: range1993326
display id: range1993326
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 955
end: 1230
orientation: inline

Scar (Thr, Gly)
type: SequenceAnnotation
persistent identity: annotation1993319
display id: annotation1993319
version: 1
top level: SP-AntiNIP
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157031/annotation1993319/range1993319/1

https://synbiohub.org/public/igem/BBa_K157031/annotation1993319/range1993319/1
type: Range
persistent identity: range1993319
display id: range1993319
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 82
end: 87
orientation: inline

Sc-Fv_anti-NIP
type: SequenceAnnotation
persistent identity: annotation1993320
display id: annotation1993320
version: 1
top level: SP-AntiNIP
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157031/annotation1993320/range1993320/1

https://synbiohub.org/public/igem/BBa_K157031/annotation1993320/range1993320/1
type: Range
persistent identity: range1993320
display id: range1993320
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 88
end: 822
orientation: inline

SP
type: SequenceAnnotation
persistent identity: annotation1993318
display id: annotation1993318
version: 1
top level: SP-AntiNIP
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157031/annotation1993318/range1993318/1

https://synbiohub.org/public/igem/BBa_K157031/annotation1993318/range1993318/1
type: Range
persistent identity: range1993318
display id: range1993318
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 10
end: 81
orientation: inline

SP-Lipocal
type: ComponentDefinition
persistent identity: BBa K157036
display id: BBa_K157036
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: SP-Lipocalin-GGGSlinker-Tm-Fluolinker-cCFP
creator: iGEM Team Freiburg 2008
created: 2008-10-25T11:00:00Z
modified: 2015-05-08T01:10:55Z
discontinued: false
dominant: false
experience: experience/None
group u list: _232_
m user id: 0
owner id: 1673
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: A composite part consisting of the signal peptide of Erbb1/EGF-receptor, scFV-antiNIP, GGGSflexlinker (extracellular part), the trans-membrane region of the Erbb1/EGF-receptor and the C-terminal part of the split fluorophor YFP with a fluolinker joined to its N-Terminus (intracellular part).
mutable notes: none
mutable provenance: Derived by cloning of the parts BBa_K157001, BBa_K157004, BBa_K157013, BBa_K157002, BBa_K157009 and BBa_K157005. For further information see the description of the parts mentioned above.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: Lipocalin
sequence annotation: AgeI
sequence annotation: Scar (Thr, Gly)
sequence annotation: C-Term_CFP
sequence annotation: Fluolinker
sequence annotation: Tm
sequence annotation: GGGS-Linker
sequence annotation: NgoMI
sequence annotation: SP
sequence annotation: SP-Lipocalin-GGGSlinker-Tm-Fluolinker-cCFP
sequence: https://synbiohub.org/public/igem/BBa_K157036_sequence/1

Lipocalin
type: SequenceAnnotation
persistent identity: annotation1993400
display id: annotation1993400
version: 1
top level: SP-Lipocal
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157036/annotation1993400/range1993400/1

https://synbiohub.org/public/igem/BBa_K157036/annotation1993400/range1993400/1
type: Range
persistent identity: range1993400
display id: range1993400
version: 1
direction: direction/forward
top level: SP-Lipocal
start: 88
end: 609
orientation: inline

AgeI
type: SequenceAnnotation
persistent identity: annotation1993408
display id: annotation1993408
version: 1
top level: SP-Lipocal
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157036/annotation1993408/range1993408/1

https://synbiohub.org/public/igem/BBa_K157036/annotation1993408/range1993408/1
type: Range
persistent identity: range1993408
display id: range1993408
version: 1
direction: direction/forward
top level: SP-Lipocal
start: 1042
end: 1047
orientation: inline

Scar (Thr, Gly)
type: SequenceAnnotation
persistent identity: annotation1993405
display id: annotation1993405
version: 1
top level: SP-Lipocal
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157036/annotation1993405/range1993405/1

https://synbiohub.org/public/igem/BBa_K157036/annotation1993405/range1993405/1
type: Range
persistent identity: range1993405
display id: range1993405
version: 1
direction: direction/forward
top level: SP-Lipocal
start: 736
end: 741
orientation: inline

C-Term_CFP
type: SequenceAnnotation
persistent identity: annotation1993395
display id: annotation1993395
version: 1
top level: SP-Lipocal
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157036/annotation1993395/range1993395/1

https://synbiohub.org/public/igem/BBa_K157036/annotation1993395/range1993395/1
type: Range
persistent identity: range1993395
display id: range1993395
version: 1
direction: direction/forward
top level: SP-Lipocal
start: 799
end: 1041
orientation: inline

Scar (Thr, Gly)
type: SequenceAnnotation
persistent identity: annotation1993399
display id: annotation1993399
version: 1
top level: SP-Lipocal
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157036/annotation1993399/range1993399/1

https://synbiohub.org/public/igem/BBa_K157036/annotation1993399/range1993399/1
type: Range
persistent identity: range1993399
display id: range1993399
version: 1
direction: direction/forward
top level: SP-Lipocal
start: 82
end: 87
orientation: inline

Fluolinker
type: SequenceAnnotation
persistent identity: annotation1993406
display id: annotation1993406
version: 1
top level: SP-Lipocal
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157036/annotation1993406/range1993406/1

https://synbiohub.org/public/igem/BBa_K157036/annotation1993406/range1993406/1
type: Range
persistent identity: range1993406
display id: range1993406
version: 1
direction: direction/forward
top level: SP-Lipocal
start: 742
end: 792
orientation: inline

Tm
type: SequenceAnnotation
persistent identity: annotation1993404
display id: annotation1993404
version: 1
top level: SP-Lipocal
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157036/annotation1993404/range1993404/1

https://synbiohub.org/public/igem/BBa_K157036/annotation1993404/range1993404/1
type: Range
persistent identity: range1993404
display id: range1993404
version: 1
direction: direction/forward
top level: SP-Lipocal
start: 667
end: 735
orientation: inline

GGGS-Linker
type: SequenceAnnotation
persistent identity: annotation1993402
display id: annotation1993402
version: 1
top level: SP-Lipocal
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157036/annotation1993402/range1993402/1

https://synbiohub.org/public/igem/BBa_K157036/annotation1993402/range1993402/1
type: Range
persistent identity: range1993402
display id: range1993402
version: 1
direction: direction/forward
top level: SP-Lipocal
start: 616
end: 660
orientation: inline

NgoMI
type: SequenceAnnotation
persistent identity: annotation1993397
display id: annotation1993397
version: 1
top level: SP-Lipocal
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157036/annotation1993397/range1993397/1

https://synbiohub.org/public/igem/BBa_K157036/annotation1993397/range1993397/1
type: Range
persistent identity: range1993397
display id: range1993397
version: 1
direction: direction/forward
top level: SP-Lipocal
start: 4
end: 9
orientation: inline

Scar (Thr, Gly)
type: SequenceAnnotation
persistent identity: annotation1993403
display id: annotation1993403
version: 1
top level: SP-Lipocal
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157036/annotation1993403/range1993403/1

https://synbiohub.org/public/igem/BBa_K157036/annotation1993403/range1993403/1
type: Range
persistent identity: range1993403
display id: range1993403
version: 1
direction: direction/forward
top level: SP-Lipocal
start: 661
end: 666
orientation: inline

Scar (Thr, Gly)
type: SequenceAnnotation
persistent identity: annotation1993401
display id: annotation1993401
version: 1
top level: SP-Lipocal
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157036/annotation1993401/range1993401/1

https://synbiohub.org/public/igem/BBa_K157036/annotation1993401/range1993401/1
type: Range
persistent identity: range1993401
display id: range1993401
version: 1
direction: direction/forward
top level: SP-Lipocal
start: 610
end: 615
orientation: inline

SP
type: SequenceAnnotation
persistent identity: annotation1993398
display id: annotation1993398
version: 1
top level: SP-Lipocal
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157036/annotation1993398/range1993398/1

https://synbiohub.org/public/igem/BBa_K157036/annotation1993398/range1993398/1
type: Range
persistent identity: range1993398
display id: range1993398
version: 1
direction: direction/forward
top level: SP-Lipocal
start: 10
end: 81
orientation: inline

SP-Lipocalin-GGGSlinker-Tm-Fluolinker-cCFP
type: SequenceAnnotation
persistent identity: annotation1993396
display id: annotation1993396
version: 1
top level: SP-Lipocal
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K157036/annotation1993396/range1993396/1

https://synbiohub.org/public/igem/BBa_K157036/annotation1993396/range1993396/1
type: Range
persistent identity: range1993396
display id: range1993396
version: 1
direction: direction/forward
top level: SP-Lipocal
start: 1
end: 1047
orientation: inline

Scar (Thr, Gly)
type: SequenceAnnotation
persistent identity: annotation1993407
display id: annotation1993407
version: 1
top level: SP-Lipocal
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157036/annotation1993407/range1993407/1

https://synbiohub.org/public/igem/BBa_K157036/annotation1993407/range1993407/1
type: Range
persistent identity: range1993407
display id: range1993407
version: 1
direction: direction/forward
top level: SP-Lipocal
start: 793
end: 798
orientation: inline

SP-Lipocal
type: ComponentDefinition
persistent identity: BBa K157032
display id: BBa_K157032
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: SP-Lipocalin-GGGSlinker-Tm-betaLactamase(Bla1)
creator: iGEM Team Freiburg 2008
created: 2008-10-25T11:00:00Z
modified: 2015-05-08T01:10:55Z
discontinued: false
dominant: false
experience: experience/None
group u list: _232_
m user id: 0
owner id: 1673
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Part consists of the signal peptide of Erbb1/EGF-receptor, scFV-antiNIP, GGGSflexlinker (extracellular part), the trans-membrane region of the Erbb1/EGF-receptor and the N-terminal part of the split enzyme beta-Lactamase (intracellular part).
mutable notes: none
mutable provenance: Derived by cloning of the parts BBa_K157001, BBa_K157004, BBa_K157013, BBa_K157002 and BBa_I757011. For further information see the description of the parts mentioned above.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: SP-Lipocalin-GGGSlinker-Tm-betaLactamase(Bla1)
sequence annotation: GGGS-Linker
sequence annotation: Scar (Thr, Gly)
sequence annotation: Bla1
sequence annotation: AgeI
sequence annotation: NgoMI
sequence annotation: Lipocalin
sequence annotation: Tm
sequence annotation: SP
sequence: https://synbiohub.org/public/igem/BBa_K157032_sequence/1

SP-Lipocalin-GGGSlinker-Tm-betaLactamase(Bla1)
type: SequenceAnnotation
persistent identity: annotation1990271
display id: annotation1990271
version: 1
top level: SP-Lipocal
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K157032/annotation1990271/range1990271/1

https://synbiohub.org/public/igem/BBa_K157032/annotation1990271/range1990271/1
type: Range
persistent identity: range1990271
display id: range1990271
version: 1
direction: direction/forward
top level: SP-Lipocal
start: 1
end: 1272
orientation: inline

GGGS-Linker
type: SequenceAnnotation
persistent identity: annotation1993336
display id: annotation1993336
version: 1
top level: SP-Lipocal
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157032/annotation1993336/range1993336/1

https://synbiohub.org/public/igem/BBa_K157032/annotation1993336/range1993336/1
type: Range
persistent identity: range1993336
display id: range1993336
version: 1
direction: direction/forward
top level: SP-Lipocal
start: 616
end: 660
orientation: inline

Scar (Thr, Gly)
type: SequenceAnnotation
persistent identity: annotation1993339
display id: annotation1993339
version: 1
top level: SP-Lipocal
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157032/annotation1993339/range1993339/1

https://synbiohub.org/public/igem/BBa_K157032/annotation1993339/range1993339/1
type: Range
persistent identity: range1993339
display id: range1993339
version: 1
direction: direction/forward
top level: SP-Lipocal
start: 736
end: 741
orientation: inline

Scar (Thr, Gly)
type: SequenceAnnotation
persistent identity: annotation1993335
display id: annotation1993335
version: 1
top level: SP-Lipocal
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157032/annotation1993335/range1993335/1

https://synbiohub.org/public/igem/BBa_K157032/annotation1993335/range1993335/1
type: Range
persistent identity: range1993335
display id: range1993335
version: 1
direction: direction/forward
top level: SP-Lipocal
start: 610
end: 615
orientation: inline

Scar (Thr, Gly)
type: SequenceAnnotation
persistent identity: annotation1993337
display id: annotation1993337
version: 1
top level: SP-Lipocal
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157032/annotation1993337/range1993337/1

https://synbiohub.org/public/igem/BBa_K157032/annotation1993337/range1993337/1
type: Range
persistent identity: range1993337
display id: range1993337
version: 1
direction: direction/forward
top level: SP-Lipocal
start: 661
end: 666
orientation: inline

Bla1
type: SequenceAnnotation
persistent identity: annotation1993340
display id: annotation1993340
version: 1
top level: SP-Lipocal
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157032/annotation1993340/range1993340/1

https://synbiohub.org/public/igem/BBa_K157032/annotation1993340/range1993340/1
type: Range
persistent identity: range1993340
display id: range1993340
version: 1
direction: direction/forward
top level: SP-Lipocal
start: 742
end: 1266
orientation: inline

AgeI
type: SequenceAnnotation
persistent identity: annotation1990270
display id: annotation1990270
version: 1
top level: SP-Lipocal
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157032/annotation1990270/range1990270/1

https://synbiohub.org/public/igem/BBa_K157032/annotation1990270/range1990270/1
type: Range
persistent identity: range1990270
display id: range1990270
version: 1
direction: direction/forward
top level: SP-Lipocal
start: 1267
end: 1272
orientation: inline

Scar (Thr, Gly)
type: SequenceAnnotation
persistent identity: annotation1993333
display id: annotation1993333
version: 1
top level: SP-Lipocal
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157032/annotation1993333/range1993333/1

https://synbiohub.org/public/igem/BBa_K157032/annotation1993333/range1993333/1
type: Range
persistent identity: range1993333
display id: range1993333
version: 1
direction: direction/forward
top level: SP-Lipocal
start: 82
end: 87
orientation: inline

NgoMI
type: SequenceAnnotation
persistent identity: annotation1990269
display id: annotation1990269
version: 1
top level: SP-Lipocal
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157032/annotation1990269/range1990269/1

https://synbiohub.org/public/igem/BBa_K157032/annotation1990269/range1990269/1
type: Range
persistent identity: range1990269
display id: range1990269
version: 1
direction: direction/forward
top level: SP-Lipocal
start: 4
end: 9
orientation: inline

Lipocalin
type: SequenceAnnotation
persistent identity: annotation1993334
display id: annotation1993334
version: 1
top level: SP-Lipocal
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157032/annotation1993334/range1993334/1

https://synbiohub.org/public/igem/BBa_K157032/annotation1993334/range1993334/1
type: Range
persistent identity: range1993334
display id: range1993334
version: 1
direction: direction/forward
top level: SP-Lipocal
start: 88
end: 609
orientation: inline

Tm
type: SequenceAnnotation
persistent identity: annotation1993338
display id: annotation1993338
version: 1
top level: SP-Lipocal
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157032/annotation1993338/range1993338/1

https://synbiohub.org/public/igem/BBa_K157032/annotation1993338/range1993338/1
type: Range
persistent identity: range1993338
display id: range1993338
version: 1
direction: direction/forward
top level: SP-Lipocal
start: 667
end: 735
orientation: inline

SP
type: SequenceAnnotation
persistent identity: annotation1993332
display id: annotation1993332
version: 1
top level: SP-Lipocal
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157032/annotation1993332/range1993332/1

https://synbiohub.org/public/igem/BBa_K157032/annotation1993332/range1993332/1
type: Range
persistent identity: range1993332
display id: range1993332
version: 1
direction: direction/forward
top level: SP-Lipocal
start: 10
end: 81
orientation: inline

Tm-betaLac
type: ComponentDefinition
persistent identity: BBa K157019
display id: BBa_K157019
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Tm-betaLactamase(Bla2)
creator: iGEM Team Freiburg 2008
created: 2008-10-25T11:00:00Z
modified: 2015-05-08T01:10:54Z
discontinued: false
dominant: false
experience: experience/None
group u list: _232_
m user id: 0
owner id: 1673
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Second fragment of beta-Lactamase fused to the trans-membrane region of the Erbb1/EGF-receptor.
mutable notes: none
mutable provenance: Derived by cloning of the parts BBa_K157002, BBa_I757012. For further information see the description of the parts mentioned above.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: AgeI
sequence annotation: Tm-betaLactamase(Bla2)
sequence annotation: Tm
sequence annotation: Scar (Thr, Gly)
sequence annotation: Bla2
sequence annotation: NgoMI
sequence: https://synbiohub.org/public/igem/BBa_K157019_sequence/1

AgeI
type: SequenceAnnotation
persistent identity: annotation1989992
display id: annotation1989992
version: 1
top level: Tm-betaLac
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157019/annotation1989992/range1989992/1

https://synbiohub.org/public/igem/BBa_K157019/annotation1989992/range1989992/1
type: Range
persistent identity: range1989992
display id: range1989992
version: 1
direction: direction/forward
top level: Tm-betaLac
start: 355
end: 360
orientation: inline

Tm-betaLactamase(Bla2)
type: SequenceAnnotation
persistent identity: annotation1989993
display id: annotation1989993
version: 1
top level: Tm-betaLac
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K157019/annotation1989993/range1989993/1

https://synbiohub.org/public/igem/BBa_K157019/annotation1989993/range1989993/1
type: Range
persistent identity: range1989993
display id: range1989993
version: 1
direction: direction/forward
top level: Tm-betaLac
start: 1
end: 360
orientation: inline

Tm
type: SequenceAnnotation
persistent identity: annotation1991465
display id: annotation1991465
version: 1
top level: Tm-betaLac
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157019/annotation1991465/range1991465/1

https://synbiohub.org/public/igem/BBa_K157019/annotation1991465/range1991465/1
type: Range
persistent identity: range1991465
display id: range1991465
version: 1
direction: direction/forward
top level: Tm-betaLac
start: 10
end: 78
orientation: inline

Scar (Thr, Gly)
type: SequenceAnnotation
persistent identity: annotation1991466
display id: annotation1991466
version: 1
top level: Tm-betaLac
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157019/annotation1991466/range1991466/1

https://synbiohub.org/public/igem/BBa_K157019/annotation1991466/range1991466/1
type: Range
persistent identity: range1991466
display id: range1991466
version: 1
direction: direction/forward
top level: Tm-betaLac
start: 79
end: 84
orientation: inline

Bla2
type: SequenceAnnotation
persistent identity: annotation1991503
display id: annotation1991503
version: 1
top level: Tm-betaLac
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157019/annotation1991503/range1991503/1

https://synbiohub.org/public/igem/BBa_K157019/annotation1991503/range1991503/1
type: Range
persistent identity: range1991503
display id: range1991503
version: 1
direction: direction/forward
top level: Tm-betaLac
start: 85
end: 354
orientation: inline

NgoMI
type: SequenceAnnotation
persistent identity: annotation1989991
display id: annotation1989991
version: 1
top level: Tm-betaLac
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157019/annotation1989991/range1989991/1

https://synbiohub.org/public/igem/BBa_K157019/annotation1989991/range1989991/1
type: Range
persistent identity: range1989991
display id: range1989991
version: 1
direction: direction/forward
top level: Tm-betaLac
start: 4
end: 9
orientation: inline

BBa_K525009
type: ComponentDefinition
persistent identity: BBa K525009
display id: BBa_K525009
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Fusion Protein of S-Layer SgsE and LigA with His-Tag
creator: Manuel Wittchen
created: 2011-10-24T11:00:00Z
modified: 2015-05-08T01:12:34Z
discontinued: false
dominant: false
experience: experience/None
group u list: _690_
m user id: 0
owner id: 8718
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Fusion Protein of S-Layer SgsE and LigA with His-Tag
mutable notes: This part was assembled by Freiburg Standard Assembly (RFC 25)
mutable provenance: SgsE was synthesized, LigA with His-Tag created by PCR. LigA is the natural ligase A of E. coli.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: NogMIV
sequence annotation: K525740
sequence annotation: Freiburg scar
sequence annotation: T7 promotor
sequence annotation: K525303
sequence annotation: SgsE domain
sequence annotation: B0034
sequence annotation: lac operator
sequence annotation: His-Tag
sequence annotation: ATG
sequence annotation: LigA domain
sequence: https://synbiohub.org/public/igem/BBa_K525009_sequence/1

NogMIV
type: SequenceAnnotation
persistent identity: annotation2160514
display id: annotation2160514
version: 1
top level: BBa_K525009
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K525009/annotation2160514/range2160514/1

https://synbiohub.org/public/igem/BBa_K525009/annotation2160514/range2160514/1
type: Range
persistent identity: range2160514
display id: range2160514
version: 1
direction: direction/forward
top level: BBa_K525009
start: 76
end: 82
orientation: inline

K525740
type: SequenceAnnotation
persistent identity: annotation2160516
display id: annotation2160516
version: 1
top level: BBa_K525009
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K525009/annotation2160516/range2160516/1

https://synbiohub.org/public/igem/BBa_K525009/annotation2160516/range2160516/1
type: Range
persistent identity: range2160516
display id: range2160516
version: 1
direction: direction/forward
top level: BBa_K525009
start: 2410
end: 4434
orientation: inline

Freiburg scar
type: SequenceAnnotation
persistent identity: annotation2160515
display id: annotation2160515
version: 1
top level: BBa_K525009
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K525009/annotation2160515/range2160515/1

https://synbiohub.org/public/igem/BBa_K525009/annotation2160515/range2160515/1
type: Range
persistent identity: range2160515
display id: range2160515
version: 1
direction: direction/forward
top level: BBa_K525009
start: 2401
end: 2407
orientation: inline

T7 promotor
type: SequenceAnnotation
persistent identity: annotation2160508
display id: annotation2160508
version: 1
top level: BBa_K525009
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K525009/annotation2160508/range2160508/1

https://synbiohub.org/public/igem/BBa_K525009/annotation2160508/range2160508/1
type: Range
persistent identity: range2160508
display id: range2160508
version: 1
direction: direction/forward
top level: BBa_K525009
start: 1
end: 20
orientation: inline

K525303
type: SequenceAnnotation
persistent identity: annotation2160509
display id: annotation2160509
version: 1
top level: BBa_K525009
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K525009/annotation2160509/range2160509/1

https://synbiohub.org/public/igem/BBa_K525009/annotation2160509/range2160509/1
type: Range
persistent identity: range2160509
display id: range2160509
version: 1
direction: direction/forward
top level: BBa_K525009
start: 1
end: 2409
orientation: inline

SgsE domain
type: SequenceAnnotation
persistent identity: annotation2160513
display id: annotation2160513
version: 1
top level: BBa_K525009
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K525009/annotation2160513/range2160513/1

https://synbiohub.org/public/igem/BBa_K525009/annotation2160513/range2160513/1
type: Range
persistent identity: range2160513
display id: range2160513
version: 1
direction: direction/forward
top level: BBa_K525009
start: 73
end: 2409
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation2160511
display id: annotation2160511
version: 1
top level: BBa_K525009
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K525009/annotation2160511/range2160511/1

https://synbiohub.org/public/igem/BBa_K525009/annotation2160511/range2160511/1
type: Range
persistent identity: range2160511
display id: range2160511
version: 1
direction: direction/forward
top level: BBa_K525009
start: 53
end: 64
orientation: inline

lac operator
type: SequenceAnnotation
persistent identity: annotation2160510
display id: annotation2160510
version: 1
top level: BBa_K525009
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K525009/annotation2160510/range2160510/1

https://synbiohub.org/public/igem/BBa_K525009/annotation2160510/range2160510/1
type: Range
persistent identity: range2160510
display id: range2160510
version: 1
direction: direction/forward
top level: BBa_K525009
start: 20
end: 44
orientation: inline

His-Tag
type: SequenceAnnotation
persistent identity: annotation2160518
display id: annotation2160518
version: 1
top level: BBa_K525009
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K525009/annotation2160518/range2160518/1

https://synbiohub.org/public/igem/BBa_K525009/annotation2160518/range2160518/1
type: Range
persistent identity: range2160518
display id: range2160518
version: 1
direction: direction/forward
top level: BBa_K525009
start: 4417
end: 4434
orientation: inline

ATG
type: SequenceAnnotation
persistent identity: annotation2160512
display id: annotation2160512
version: 1
top level: BBa_K525009
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K525009/annotation2160512/range2160512/1

https://synbiohub.org/public/igem/BBa_K525009/annotation2160512/range2160512/1
type: Range
persistent identity: range2160512
display id: range2160512
version: 1
direction: direction/forward
top level: BBa_K525009
start: 73
end: 75
orientation: inline

LigA domain
type: SequenceAnnotation
persistent identity: annotation2160517
display id: annotation2160517
version: 1
top level: BBa_K525009
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K525009/annotation2160517/range2160517/1

https://synbiohub.org/public/igem/BBa_K525009/annotation2160517/range2160517/1
type: Range
persistent identity: range2160517
display id: range2160517
version: 1
direction: direction/forward
top level: BBa_K525009
start: 2407
end: 4416
orientation: inline

cspB
type: ComponentDefinition
persistent identity: BBa K525123
display id: BBa_K525123
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: S-layer cspB from Corynebacterium glutamicum with lipid anchor and PT7 and RBS
creator: Anna Drong
created: 2011-09-10T11:00:00Z
modified: 2015-05-08T01:12:34Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _690_
m user id: 0
owner id: 8543
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: SL-BF-3 mit pT7 und RBS; Lipid anchor
mutable notes: PCR
mutable provenance: Brevibacterium flavum, PCR
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Promoter
sequence annotation: RBS
sequence annotation: Lipid anchor
sequence annotation: S-layer
sequence: https://synbiohub.org/public/igem/BBa_K525123_sequence/1

Promoter
type: SequenceAnnotation
persistent identity: annotation2128108
display id: annotation2128108
version: 1
top level: cspB
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K525123/annotation2128108/range2128108/1

https://synbiohub.org/public/igem/BBa_K525123/annotation2128108/range2128108/1
type: Range
persistent identity: range2128108
display id: range2128108
version: 1
direction: direction/forward
top level: cspB
start: 1
end: 22
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2127134
display id: annotation2127134
version: 1
top level: cspB
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K525123/annotation2127134/range2127134/1

https://synbiohub.org/public/igem/BBa_K525123/annotation2127134/range2127134/1
type: Range
persistent identity: range2127134
display id: range2127134
version: 1
direction: direction/forward
top level: cspB
start: 23
end: 34
orientation: inline

Lipid anchor
type: SequenceAnnotation
persistent identity: annotation2127316
display id: annotation2127316
version: 1
top level: cspB
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K525123/annotation2127316/range2127316/1

https://synbiohub.org/public/igem/BBa_K525123/annotation2127316/range2127316/1
type: Range
persistent identity: range2127316
display id: range2127316
version: 1
direction: direction/forward
top level: cspB
start: 1348
end: 1423
orientation: inline

S-layer
type: SequenceAnnotation
persistent identity: annotation2127317
display id: annotation2127317
version: 1
top level: cspB
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K525123/annotation2127317/range2127317/1

https://synbiohub.org/public/igem/BBa_K525123/annotation2127317/range2127317/1
type: Range
persistent identity: range2127317
display id: range2127317
version: 1
direction: direction/forward
top level: cspB
start: 35
end: 1347
orientation: inline

BBa_K157007
type: ComponentDefinition
persistent identity: BBa K157007
display id: BBa_K157007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Split-Venus-cYFP
creator: iGEM Team Freiburg 2008
created: 2008-10-25T11:00:00Z
modified: 2015-05-08T01:10:54Z
discontinued: false
dominant: false
experience: experience/None
group u list: _232_
m user id: 0
owner id: 1673
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: C-terminal fragment (Amino acids157-237) of the monomeric[5] yellow fluorescent protein variant ???Venus??? [4], designed for fusion/BiFC[1,3]. Reassembly with Part Bba_K157008 (Split-Venus-nYFP) generates the complete, working YFP ???Venus???, whereas combination with the n-terminal fragment (Part Bba_K157006) of the monomeric cyan fluorescent Protein ???CeruleanR206K??? (Part Bba_I757009) results in green fluorescence[2]. - [1] Chang-Deng Hu, Yurii Chinenov, Tom K. Kerppola: ???Visualization of Interactions among bZIP and Rel Family Proteins in Living Cells Using Bimolecular Fluorescence Complementation???, Molecular Cell, Vol. 9, 789???798, April, 2002 - [2] Chang Deng Hu, Tom K. Kerppola: ???Simultaneous visualization of multiple protein interactions in living cells using multicolor fluorescence complementation analysis???, Nat Biotechnol. 2003 May; 21(5):539-545 (doi:10. 1038/nbt816) -[3] Tom K. Kerppola: ???Design and implementation of bimolecular fluorescence complementation (BiFC) assays for the visualization of protein interactions in living cells???, Nat Protoc. 2006;1(3):1278-1286 (doi:10.1038/nprot.2006.201) -[4]Nagai, T. et al. ???A variant of yellow fluorescent protein with fast and efficient maturation for cell-biological applications??? J. Biol. Chem. 276, 29188-29194, 2001 -[5]Roger Y. Tsien et al. ???Creating new fluorescent probes for cell biology???, Nature Biotechnology Reviews, Vol. 3, 906-918, 2002
mutable notes: Between BioBrick 1.0 sites this part is flanked 5' by NgoMI(=NgoMIV) and 3' AgeI(=PinAI) to facilitate in frame cloning for protein fusions.
mutable provenance: Gene synthesis by GeneArt, optimized for expression in homo sapiens.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: C-terminal portion of split venus YFP
sequence: https://synbiohub.org/public/igem/BBa_K157007_sequence/1

C-terminal portion of split venus YFP
type: SequenceAnnotation
persistent identity: annotation2064735
display id: annotation2064735
version: 1
top level: BBa_K157007
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157007/annotation2064735/range2064735/1

https://synbiohub.org/public/igem/BBa_K157007/annotation2064735/range2064735/1
type: Range
persistent identity: range2064735
display id: range2064735
version: 1
direction: direction/forward
top level: BBa_K157007
start: 1
end: 243
orientation: inline

SgsE | CFP
type: ComponentDefinition
persistent identity: BBa K525306
display id: BBa_K525306
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Fusion Protein of S-Layer SgsE and mCerulean
creator: Timo Wolf
created: 2011-09-09T11:00:00Z
modified: 2016-01-26T09:40:40Z
discontinued: false
dominant: false
experience: experience/None
group u list: _690_
m user id: 4206
owner id: 6668
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: Fusion Protein of S-Layer SgsE and mCerulean
mutable notes: Fusion Protein of BBa_K525303 and BBa_J18930
mutable provenance: Fusion Protein of S-Layer SgsE and mCerulean
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: K525301
sequence annotation: RBS
sequence annotation: TAA
sequence annotation: mCerulean
sequence annotation: AgeI
sequence annotation: J18930
sequence annotation: B0034
sequence annotation: SgsE domain
sequence annotation: NgoMIV
sequence annotation: lac operator
sequence annotation: ATG
sequence annotation: T7 promoter
sequence annotation: Freiburg Scar
sequence: https://synbiohub.org/public/igem/BBa_K525306_sequence/1

K525301
type: SequenceAnnotation
persistent identity: annotation2128599
display id: annotation2128599
version: 1
top level: SgsE | CFP
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K525306/annotation2128599/range2128599/1

https://synbiohub.org/public/igem/BBa_K525306/annotation2128599/range2128599/1
type: Range
persistent identity: range2128599
display id: range2128599
version: 1
direction: direction/forward
top level: SgsE | CFP
start: 73
end: 2400
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2128598
display id: annotation2128598
version: 1
top level: SgsE | CFP
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K525306/annotation2128598/range2128598/1

https://synbiohub.org/public/igem/BBa_K525306/annotation2128598/range2128598/1
type: Range
persistent identity: range2128598
display id: range2128598
version: 1
direction: direction/forward
top level: SgsE | CFP
start: 56
end: 59
orientation: inline

TAA
type: SequenceAnnotation
persistent identity: annotation2128607
display id: annotation2128607
version: 1
top level: SgsE | CFP
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K525306/annotation2128607/range2128607/1

https://synbiohub.org/public/igem/BBa_K525306/annotation2128607/range2128607/1
type: Range
persistent identity: range2128607
display id: range2128607
version: 1
direction: direction/forward
top level: SgsE | CFP
start: 3126
end: 3129
orientation: inline

mCerulean
type: SequenceAnnotation
persistent identity: annotation2128603
display id: annotation2128603
version: 1
top level: SgsE | CFP
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K525306/annotation2128603/range2128603/1

https://synbiohub.org/public/igem/BBa_K525306/annotation2128603/range2128603/1
type: Range
persistent identity: range2128603
display id: range2128603
version: 1
direction: direction/forward
top level: SgsE | CFP
start: 2406
end: 3129
orientation: inline

AgeI
type: SequenceAnnotation
persistent identity: annotation2128605
display id: annotation2128605
version: 1
top level: SgsE | CFP
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K525306/annotation2128605/range2128605/1

https://synbiohub.org/public/igem/BBa_K525306/annotation2128605/range2128605/1
type: Range
persistent identity: range2128605
display id: range2128605
version: 1
direction: direction/forward
top level: SgsE | CFP
start: 3121
end: 3126
orientation: inline

J18930
type: SequenceAnnotation
persistent identity: annotation2128602
display id: annotation2128602
version: 1
top level: SgsE | CFP
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K525306/annotation2128602/range2128602/1

https://synbiohub.org/public/igem/BBa_K525306/annotation2128602/range2128602/1
type: Range
persistent identity: range2128602
display id: range2128602
version: 1
direction: direction/forward
top level: SgsE | CFP
start: 2406
end: 3129
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation2128588
display id: annotation2128588
version: 1
top level: SgsE | CFP
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K525306/annotation2128588/range2128588/1

https://synbiohub.org/public/igem/BBa_K525306/annotation2128588/range2128588/1
type: Range
persistent identity: range2128588
display id: range2128588
version: 1
direction: direction/forward
top level: SgsE | CFP
start: 53
end: 64
orientation: inline

SgsE domain
type: SequenceAnnotation
persistent identity: annotation2128600
display id: annotation2128600
version: 1
top level: SgsE | CFP
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K525306/annotation2128600/range2128600/1

https://synbiohub.org/public/igem/BBa_K525306/annotation2128600/range2128600/1
type: Range
persistent identity: range2128600
display id: range2128600
version: 1
direction: direction/forward
top level: SgsE | CFP
start: 73
end: 2400
orientation: inline

NgoMIV
type: SequenceAnnotation
persistent identity: annotation2128604
display id: annotation2128604
version: 1
top level: SgsE | CFP
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K525306/annotation2128604/range2128604/1

https://synbiohub.org/public/igem/BBa_K525306/annotation2128604/range2128604/1
type: Range
persistent identity: range2128604
display id: range2128604
version: 1
direction: direction/forward
top level: SgsE | CFP
start: 76
end: 81
orientation: inline

lac operator
type: SequenceAnnotation
persistent identity: annotation2128587
display id: annotation2128587
version: 1
top level: SgsE | CFP
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K525306/annotation2128587/range2128587/1

https://synbiohub.org/public/igem/BBa_K525306/annotation2128587/range2128587/1
type: Range
persistent identity: range2128587
display id: range2128587
version: 1
direction: direction/forward
top level: SgsE | CFP
start: 20
end: 44
orientation: inline

ATG
type: SequenceAnnotation
persistent identity: annotation2128606
display id: annotation2128606
version: 1
top level: SgsE | CFP
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K525306/annotation2128606/range2128606/1

https://synbiohub.org/public/igem/BBa_K525306/annotation2128606/range2128606/1
type: Range
persistent identity: range2128606
display id: range2128606
version: 1
direction: direction/forward
top level: SgsE | CFP
start: 73
end: 75
orientation: inline

T7 promoter
type: SequenceAnnotation
persistent identity: annotation2128585
display id: annotation2128585
version: 1
top level: SgsE | CFP
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K525306/annotation2128585/range2128585/1

https://synbiohub.org/public/igem/BBa_K525306/annotation2128585/range2128585/1
type: Range
persistent identity: range2128585
display id: range2128585
version: 1
direction: direction/forward
top level: SgsE | CFP
start: 1
end: 20
orientation: inline

Freiburg Scar
type: SequenceAnnotation
persistent identity: annotation2128601
display id: annotation2128601
version: 1
top level: SgsE | CFP
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K525306/annotation2128601/range2128601/1

https://synbiohub.org/public/igem/BBa_K525306/annotation2128601/range2128601/1
type: Range
persistent identity: range2128601
display id: range2128601
version: 1
direction: direction/forward
top level: SgsE | CFP
start: 2401
end: 2406
orientation: inline

BBa_K1909004
type: ComponentDefinition
persistent identity: BBa K1909004
display id: BBa_K1909004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: NOD1 LRR Domain
creator: Daniel Wedemeyer
created: 2016-10-13T11:00:00Z
modified: 2016-10-14T11:21:00Z
discontinued: false
dominant: false
group u list: _2375_
m user id: 29525
owner id: 29525
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Coming soon
mutable notes: coming soon
mutable provenance: NOD1 (ncbi: https://www.ncbi.nlm.nih.gov/gene/10392)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Changed CTG to TTG
sequence annotation: NOD1
sequence annotation: Changed GAC to GAT
sequence annotation: Changed CTG to CTA
sequence annotation: Changed CTA to TTA
sequence: https://synbiohub.org/public/igem/BBa_K1909004_sequence/1

Changed CTG to TTG
type: SequenceAnnotation
persistent identity: annotation2507291
display id: annotation2507291
version: 1
top level: BBa_K1909004
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_K1909004/annotation2507291/range2507291/1

https://synbiohub.org/public/igem/BBa_K1909004/annotation2507291/range2507291/1
type: Range
persistent identity: range2507291
display id: range2507291
version: 1
direction: direction/forward
top level: BBa_K1909004
start: 277
end: 279
orientation: inline

NOD1
type: SequenceAnnotation
persistent identity: annotation2507199
display id: annotation2507199
version: 1
top level: BBa_K1909004
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1909004/annotation2507199/range2507199/1

https://synbiohub.org/public/igem/BBa_K1909004/annotation2507199/range2507199/1
type: Range
persistent identity: range2507199
display id: range2507199
version: 1
direction: direction/forward
top level: BBa_K1909004
start: 1
end: 969
orientation: inline

Changed GAC to GAT
type: SequenceAnnotation
persistent identity: annotation2507200
display id: annotation2507200
version: 1
top level: BBa_K1909004
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_K1909004/annotation2507200/range2507200/1

https://synbiohub.org/public/igem/BBa_K1909004/annotation2507200/range2507200/1
type: Range
persistent identity: range2507200
display id: range2507200
version: 1
direction: direction/forward
top level: BBa_K1909004
start: 181
end: 183
orientation: inline

Changed CTG to TTG
type: SequenceAnnotation
persistent identity: annotation2507292
display id: annotation2507292
version: 1
top level: BBa_K1909004
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_K1909004/annotation2507292/range2507292/1

https://synbiohub.org/public/igem/BBa_K1909004/annotation2507292/range2507292/1
type: Range
persistent identity: range2507292
display id: range2507292
version: 1
direction: direction/forward
top level: BBa_K1909004
start: 700
end: 702
orientation: inline

Changed CTG to CTA
type: SequenceAnnotation
persistent identity: annotation2507294
display id: annotation2507294
version: 1
top level: BBa_K1909004
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_K1909004/annotation2507294/range2507294/1

https://synbiohub.org/public/igem/BBa_K1909004/annotation2507294/range2507294/1
type: Range
persistent identity: range2507294
display id: range2507294
version: 1
direction: direction/forward
top level: BBa_K1909004
start: 727
end: 729
orientation: inline

Changed CTA to TTA
type: SequenceAnnotation
persistent identity: annotation2507293
display id: annotation2507293
version: 1
top level: BBa_K1909004
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_K1909004/annotation2507293/range2507293/1

https://synbiohub.org/public/igem/BBa_K1909004/annotation2507293/range2507293/1
type: Range
persistent identity: range2507293
display id: range2507293
version: 1
direction: direction/forward
top level: BBa_K1909004
start: 718
end: 720
orientation: inline

SbpA | Red
type: ComponentDefinition
persistent identity: BBa K525412
display id: BBa_K525412
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Fusion Protein of S-Layer SbpA and NADP+ Oxidoreductase
creator: Anna Drong
created: 2011-09-10T11:00:00Z
modified: 2015-05-08T01:12:34Z
discontinued: false
dominant: false
experience: experience/None
group u list: _690_
m user id: 0
owner id: 8543
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: PT7 + RBS + SbpA || Red; RCU 25
mutable notes: Assembled
mutable provenance: BBa_K525404; BBa_K525499
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: ATG
sequence annotation: Freiburg Scar
sequence annotation: lac operator
sequence annotation: T7 promoter
sequence annotation: K525401
sequence annotation: Reductase
sequence annotation: B0034
sequence annotation: K525499
sequence annotation: SbpA domain
sequence annotation: RBS
sequence annotation: TAA
sequence annotation: NgoMIV
sequence annotation: AgeI
sequence: https://synbiohub.org/public/igem/BBa_K525412_sequence/1

ATG
type: SequenceAnnotation
persistent identity: annotation2136965
display id: annotation2136965
version: 1
top level: SbpA | Red
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K525412/annotation2136965/range2136965/1

https://synbiohub.org/public/igem/BBa_K525412/annotation2136965/range2136965/1
type: Range
persistent identity: range2136965
display id: range2136965
version: 1
direction: direction/forward
top level: SbpA | Red
start: 73
end: 75
orientation: inline

Freiburg Scar
type: SequenceAnnotation
persistent identity: annotation2136968
display id: annotation2136968
version: 1
top level: SbpA | Red
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K525412/annotation2136968/range2136968/1

https://synbiohub.org/public/igem/BBa_K525412/annotation2136968/range2136968/1
type: Range
persistent identity: range2136968
display id: range2136968
version: 1
direction: direction/forward
top level: SbpA | Red
start: 3193
end: 3198
orientation: inline

lac operator
type: SequenceAnnotation
persistent identity: annotation2136961
display id: annotation2136961
version: 1
top level: SbpA | Red
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K525412/annotation2136961/range2136961/1

https://synbiohub.org/public/igem/BBa_K525412/annotation2136961/range2136961/1
type: Range
persistent identity: range2136961
display id: range2136961
version: 1
direction: direction/forward
top level: SbpA | Red
start: 20
end: 44
orientation: inline

T7 promoter
type: SequenceAnnotation
persistent identity: annotation2136960
display id: annotation2136960
version: 1
top level: SbpA | Red
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K525412/annotation2136960/range2136960/1

https://synbiohub.org/public/igem/BBa_K525412/annotation2136960/range2136960/1
type: Range
persistent identity: range2136960
display id: range2136960
version: 1
direction: direction/forward
top level: SbpA | Red
start: 1
end: 20
orientation: inline

K525401
type: SequenceAnnotation
persistent identity: annotation2136964
display id: annotation2136964
version: 1
top level: SbpA | Red
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K525412/annotation2136964/range2136964/1

https://synbiohub.org/public/igem/BBa_K525412/annotation2136964/range2136964/1
type: Range
persistent identity: range2136964
display id: range2136964
version: 1
direction: direction/forward
top level: SbpA | Red
start: 73
end: 3193
orientation: inline

Reductase
type: SequenceAnnotation
persistent identity: annotation2136970
display id: annotation2136970
version: 1
top level: SbpA | Red
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K525412/annotation2136970/range2136970/1

https://synbiohub.org/public/igem/BBa_K525412/annotation2136970/range2136970/1
type: Range
persistent identity: range2136970
display id: range2136970
version: 1
direction: direction/forward
top level: SbpA | Red
start: 3199
end: 3948
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation2136962
display id: annotation2136962
version: 1
top level: SbpA | Red
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K525412/annotation2136962/range2136962/1

https://synbiohub.org/public/igem/BBa_K525412/annotation2136962/range2136962/1
type: Range
persistent identity: range2136962
display id: range2136962
version: 1
direction: direction/forward
top level: SbpA | Red
start: 53
end: 64
orientation: inline

K525499
type: SequenceAnnotation
persistent identity: annotation2136969
display id: annotation2136969
version: 1
top level: SbpA | Red
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K525412/annotation2136969/range2136969/1

https://synbiohub.org/public/igem/BBa_K525412/annotation2136969/range2136969/1
type: Range
persistent identity: range2136969
display id: range2136969
version: 1
direction: direction/forward
top level: SbpA | Red
start: 3199
end: 3948
orientation: inline

SbpA domain
type: SequenceAnnotation
persistent identity: annotation2136966
display id: annotation2136966
version: 1
top level: SbpA | Red
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K525412/annotation2136966/range2136966/1

https://synbiohub.org/public/igem/BBa_K525412/annotation2136966/range2136966/1
type: Range
persistent identity: range2136966
display id: range2136966
version: 1
direction: direction/forward
top level: SbpA | Red
start: 73
end: 3193
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2136963
display id: annotation2136963
version: 1
top level: SbpA | Red
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K525412/annotation2136963/range2136963/1

https://synbiohub.org/public/igem/BBa_K525412/annotation2136963/range2136963/1
type: Range
persistent identity: range2136963
display id: range2136963
version: 1
direction: direction/forward
top level: SbpA | Red
start: 56
end: 59
orientation: inline

TAA
type: SequenceAnnotation
persistent identity: annotation2136972
display id: annotation2136972
version: 1
top level: SbpA | Red
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K525412/annotation2136972/range2136972/1

https://synbiohub.org/public/igem/BBa_K525412/annotation2136972/range2136972/1
type: Range
persistent identity: range2136972
display id: range2136972
version: 1
direction: direction/forward
top level: SbpA | Red
start: 3946
end: 3948
orientation: inline

NgoMIV
type: SequenceAnnotation
persistent identity: annotation2136967
display id: annotation2136967
version: 1
top level: SbpA | Red
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K525412/annotation2136967/range2136967/1

https://synbiohub.org/public/igem/BBa_K525412/annotation2136967/range2136967/1
type: Range
persistent identity: range2136967
display id: range2136967
version: 1
direction: direction/forward
top level: SbpA | Red
start: 76
end: 81
orientation: inline

AgeI
type: SequenceAnnotation
persistent identity: annotation2136971
display id: annotation2136971
version: 1
top level: SbpA | Red
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K525412/annotation2136971/range2136971/1

https://synbiohub.org/public/igem/BBa_K525412/annotation2136971/range2136971/1
type: Range
persistent identity: range2136971
display id: range2136971
version: 1
direction: direction/forward
top level: SbpA | Red
start: 3940
end: 3944
orientation: inline

SP-Lipocal
type: ComponentDefinition
persistent identity: BBa K157033
display id: BBa_K157033
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: SP-Lipocalin-GGGSlinker-Tm-betaLactamase(Bla2)
creator: iGEM Team Freiburg 2008
created: 2008-10-25T11:00:00Z
modified: 2015-05-08T01:10:55Z
discontinued: false
dominant: false
experience: experience/None
group u list: _232_
m user id: 0
owner id: 1673
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Part consists of the signal peptide of Erbb1/EGF-receptor, scFV-antiNIP, GGGSflexlinker (extracellular part), the trans-membrane region of the Erbb1/EGF-receptor and the C-terminal part of the split enzyme beta-Lactamase (intracellular part).
mutable notes: none
mutable provenance: Derived by cloning of the parts BBa_K157001, BBa_K157004, BBa_K157013, BBa_K157002 and BBa_I757012. For further information see the description of the parts mentioned above.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: Scar (Thr, Gly)
sequence annotation: SP
sequence annotation: Lipocalin
sequence annotation: Tm
sequence annotation: NgoMI
sequence annotation: GGGS-Linker
sequence annotation: Bla2
sequence annotation: SP-Lipocalin-GGGSlinker-Tm-betaLactamase(Bla2)
sequence annotation: AgeI
sequence: https://synbiohub.org/public/igem/BBa_K157033_sequence/1

Scar (Thr, Gly)
type: SequenceAnnotation
persistent identity: annotation1993344
display id: annotation1993344
version: 1
top level: SP-Lipocal
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157033/annotation1993344/range1993344/1

https://synbiohub.org/public/igem/BBa_K157033/annotation1993344/range1993344/1
type: Range
persistent identity: range1993344
display id: range1993344
version: 1
direction: direction/forward
top level: SP-Lipocal
start: 610
end: 615
orientation: inline

SP
type: SequenceAnnotation
persistent identity: annotation1993341
display id: annotation1993341
version: 1
top level: SP-Lipocal
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157033/annotation1993341/range1993341/1

https://synbiohub.org/public/igem/BBa_K157033/annotation1993341/range1993341/1
type: Range
persistent identity: range1993341
display id: range1993341
version: 1
direction: direction/forward
top level: SP-Lipocal
start: 10
end: 81
orientation: inline

Lipocalin
type: SequenceAnnotation
persistent identity: annotation1993343
display id: annotation1993343
version: 1
top level: SP-Lipocal
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157033/annotation1993343/range1993343/1

https://synbiohub.org/public/igem/BBa_K157033/annotation1993343/range1993343/1
type: Range
persistent identity: range1993343
display id: range1993343
version: 1
direction: direction/forward
top level: SP-Lipocal
start: 88
end: 609
orientation: inline

Scar (Thr, Gly)
type: SequenceAnnotation
persistent identity: annotation1993342
display id: annotation1993342
version: 1
top level: SP-Lipocal
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157033/annotation1993342/range1993342/1

https://synbiohub.org/public/igem/BBa_K157033/annotation1993342/range1993342/1
type: Range
persistent identity: range1993342
display id: range1993342
version: 1
direction: direction/forward
top level: SP-Lipocal
start: 82
end: 87
orientation: inline

Tm
type: SequenceAnnotation
persistent identity: annotation1993347
display id: annotation1993347
version: 1
top level: SP-Lipocal
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157033/annotation1993347/range1993347/1

https://synbiohub.org/public/igem/BBa_K157033/annotation1993347/range1993347/1
type: Range
persistent identity: range1993347
display id: range1993347
version: 1
direction: direction/forward
top level: SP-Lipocal
start: 667
end: 735
orientation: inline

NgoMI
type: SequenceAnnotation
persistent identity: annotation1990272
display id: annotation1990272
version: 1
top level: SP-Lipocal
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157033/annotation1990272/range1990272/1

https://synbiohub.org/public/igem/BBa_K157033/annotation1990272/range1990272/1
type: Range
persistent identity: range1990272
display id: range1990272
version: 1
direction: direction/forward
top level: SP-Lipocal
start: 4
end: 9
orientation: inline

GGGS-Linker
type: SequenceAnnotation
persistent identity: annotation1993345
display id: annotation1993345
version: 1
top level: SP-Lipocal
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157033/annotation1993345/range1993345/1

https://synbiohub.org/public/igem/BBa_K157033/annotation1993345/range1993345/1
type: Range
persistent identity: range1993345
display id: range1993345
version: 1
direction: direction/forward
top level: SP-Lipocal
start: 616
end: 660
orientation: inline

Bla2
type: SequenceAnnotation
persistent identity: annotation1993349
display id: annotation1993349
version: 1
top level: SP-Lipocal
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157033/annotation1993349/range1993349/1

https://synbiohub.org/public/igem/BBa_K157033/annotation1993349/range1993349/1
type: Range
persistent identity: range1993349
display id: range1993349
version: 1
direction: direction/forward
top level: SP-Lipocal
start: 742
end: 1011
orientation: inline

SP-Lipocalin-GGGSlinker-Tm-betaLactamase(Bla2)
type: SequenceAnnotation
persistent identity: annotation1990274
display id: annotation1990274
version: 1
top level: SP-Lipocal
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K157033/annotation1990274/range1990274/1

https://synbiohub.org/public/igem/BBa_K157033/annotation1990274/range1990274/1
type: Range
persistent identity: range1990274
display id: range1990274
version: 1
direction: direction/forward
top level: SP-Lipocal
start: 1
end: 1017
orientation: inline

Scar (Thr, Gly)
type: SequenceAnnotation
persistent identity: annotation1993348
display id: annotation1993348
version: 1
top level: SP-Lipocal
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157033/annotation1993348/range1993348/1

https://synbiohub.org/public/igem/BBa_K157033/annotation1993348/range1993348/1
type: Range
persistent identity: range1993348
display id: range1993348
version: 1
direction: direction/forward
top level: SP-Lipocal
start: 736
end: 741
orientation: inline

AgeI
type: SequenceAnnotation
persistent identity: annotation1990273
display id: annotation1990273
version: 1
top level: SP-Lipocal
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157033/annotation1990273/range1990273/1

https://synbiohub.org/public/igem/BBa_K157033/annotation1990273/range1990273/1
type: Range
persistent identity: range1990273
display id: range1990273
version: 1
direction: direction/forward
top level: SP-Lipocal
start: 1012
end: 1017
orientation: inline

Scar (Thr, Gly)
type: SequenceAnnotation
persistent identity: annotation1993346
display id: annotation1993346
version: 1
top level: SP-Lipocal
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157033/annotation1993346/range1993346/1

https://synbiohub.org/public/igem/BBa_K157033/annotation1993346/range1993346/1
type: Range
persistent identity: range1993346
display id: range1993346
version: 1
direction: direction/forward
top level: SP-Lipocal
start: 661
end: 666
orientation: inline

Tm-Fluo-li
type: ComponentDefinition
persistent identity: BBa K157014
display id: BBa_K157014
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Tm-Fluo-linker-cYFP
creator: iGEM Team Freiburg 2008
created: 2008-10-25T11:00:00Z
modified: 2015-05-08T01:10:54Z
discontinued: false
dominant: false
experience: experience/None
group u list: _232_
m user id: 0
owner id: 1673
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: C-terminal part of the Split fluorophore YFP fusioned to the transmembrane region of the Erbb1/EGF-receptor with the fluolinker in between.
mutable notes: none
mutable provenance: Derived by cloning of the parts BBa_K157002, BBa_K157009 and BBa_K157007. For further information see the description of the parts mentioned above.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: Fluolinker
sequence annotation: Tm
sequence annotation: Scar (Thr, Gly)
sequence annotation: AgeI
sequence annotation: NgoMI
sequence annotation: Tm-Fluolinker-cYFP
sequence annotation: C-Term_YFP
sequence: https://synbiohub.org/public/igem/BBa_K157014_sequence/1

Fluolinker
type: SequenceAnnotation
persistent identity: annotation1990824
display id: annotation1990824
version: 1
top level: Tm-Fluo-li
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157014/annotation1990824/range1990824/1

https://synbiohub.org/public/igem/BBa_K157014/annotation1990824/range1990824/1
type: Range
persistent identity: range1990824
display id: range1990824
version: 1
direction: direction/forward
top level: Tm-Fluo-li
start: 85
end: 135
orientation: inline

Tm
type: SequenceAnnotation
persistent identity: annotation1990821
display id: annotation1990821
version: 1
top level: Tm-Fluo-li
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157014/annotation1990821/range1990821/1

https://synbiohub.org/public/igem/BBa_K157014/annotation1990821/range1990821/1
type: Range
persistent identity: range1990821
display id: range1990821
version: 1
direction: direction/forward
top level: Tm-Fluo-li
start: 10
end: 78
orientation: inline

Scar (Thr, Gly)
type: SequenceAnnotation
persistent identity: annotation1990942
display id: annotation1990942
version: 1
top level: Tm-Fluo-li
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157014/annotation1990942/range1990942/1

https://synbiohub.org/public/igem/BBa_K157014/annotation1990942/range1990942/1
type: Range
persistent identity: range1990942
display id: range1990942
version: 1
direction: direction/forward
top level: Tm-Fluo-li
start: 136
end: 141
orientation: inline

AgeI
type: SequenceAnnotation
persistent identity: annotation1989805
display id: annotation1989805
version: 1
top level: Tm-Fluo-li
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157014/annotation1989805/range1989805/1

https://synbiohub.org/public/igem/BBa_K157014/annotation1989805/range1989805/1
type: Range
persistent identity: range1989805
display id: range1989805
version: 1
direction: direction/forward
top level: Tm-Fluo-li
start: 385
end: 390
orientation: inline

NgoMI
type: SequenceAnnotation
persistent identity: annotation1989804
display id: annotation1989804
version: 1
top level: Tm-Fluo-li
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157014/annotation1989804/range1989804/1

https://synbiohub.org/public/igem/BBa_K157014/annotation1989804/range1989804/1
type: Range
persistent identity: range1989804
display id: range1989804
version: 1
direction: direction/forward
top level: Tm-Fluo-li
start: 4
end: 9
orientation: inline

Scar (Thr, Gly)
type: SequenceAnnotation
persistent identity: annotation1990890
display id: annotation1990890
version: 1
top level: Tm-Fluo-li
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157014/annotation1990890/range1990890/1

https://synbiohub.org/public/igem/BBa_K157014/annotation1990890/range1990890/1
type: Range
persistent identity: range1990890
display id: range1990890
version: 1
direction: direction/forward
top level: Tm-Fluo-li
start: 79
end: 84
orientation: inline

Tm-Fluolinker-cYFP
type: SequenceAnnotation
persistent identity: annotation1989803
display id: annotation1989803
version: 1
top level: Tm-Fluo-li
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K157014/annotation1989803/range1989803/1

https://synbiohub.org/public/igem/BBa_K157014/annotation1989803/range1989803/1
type: Range
persistent identity: range1989803
display id: range1989803
version: 1
direction: direction/forward
top level: Tm-Fluo-li
start: 1
end: 390
orientation: inline

C-Term_YFP
type: SequenceAnnotation
persistent identity: annotation1990876
display id: annotation1990876
version: 1
top level: Tm-Fluo-li
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157014/annotation1990876/range1990876/1

https://synbiohub.org/public/igem/BBa_K157014/annotation1990876/range1990876/1
type: Range
persistent identity: range1990876
display id: range1990876
version: 1
direction: direction/forward
top level: Tm-Fluo-li
start: 142
end: 384
orientation: inline

SP-AntiNIP
type: ComponentDefinition
persistent identity: BBa K157025
display id: BBa_K157025
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: SP-AntiNIP-GGGSlinker-Tm-betaLactamase(Bla2)
creator: iGEM Team Freiburg 2008
created: 2008-10-25T11:00:00Z
modified: 2015-05-08T01:10:55Z
discontinued: false
dominant: false
experience: experience/None
group u list: _232_
m user id: 0
owner id: 1673
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: A composite part consisting of the signal peptide of Erbb1/EGF-receptor, scFV-antiNIP, GGGSflexlinker (extracellular part), the trans-membrane region of the Erbb1/EGF-receptor and the C-terminal part of the split-enzyme beta-Lactamase (intracellular part).
mutable notes: none
mutable provenance: Derived by cloning of the parts BBa_K157001, BBa_K157003, BBa_K157013, BBa_K157002 and BBa_I757012. For further information see the description of the parts mentioned above.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: Sc-Fv_anti-NIP
sequence annotation: Tm
sequence annotation: AgeI
sequence annotation: SP-AntiNIP-GGGSlinker-Tm-betaLactamase(Bla2)
sequence annotation: SP
sequence annotation: Scar (Thr, Gly)
sequence annotation: Bla2
sequence annotation: GGGS-Linker
sequence annotation: NgoMI
sequence: https://synbiohub.org/public/igem/BBa_K157025_sequence/1

Sc-Fv_anti-NIP
type: SequenceAnnotation
persistent identity: annotation1992630
display id: annotation1992630
version: 1
top level: SP-AntiNIP
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157025/annotation1992630/range1992630/1

https://synbiohub.org/public/igem/BBa_K157025/annotation1992630/range1992630/1
type: Range
persistent identity: range1992630
display id: range1992630
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 88
end: 822
orientation: inline

Tm
type: SequenceAnnotation
persistent identity: annotation1992634
display id: annotation1992634
version: 1
top level: SP-AntiNIP
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157025/annotation1992634/range1992634/1

https://synbiohub.org/public/igem/BBa_K157025/annotation1992634/range1992634/1
type: Range
persistent identity: range1992634
display id: range1992634
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 880
end: 948
orientation: inline

AgeI
type: SequenceAnnotation
persistent identity: annotation1992637
display id: annotation1992637
version: 1
top level: SP-AntiNIP
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157025/annotation1992637/range1992637/1

https://synbiohub.org/public/igem/BBa_K157025/annotation1992637/range1992637/1
type: Range
persistent identity: range1992637
display id: range1992637
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 1225
end: 1230
orientation: inline

SP-AntiNIP-GGGSlinker-Tm-betaLactamase(Bla2)
type: SequenceAnnotation
persistent identity: annotation1992626
display id: annotation1992626
version: 1
top level: SP-AntiNIP
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K157025/annotation1992626/range1992626/1

https://synbiohub.org/public/igem/BBa_K157025/annotation1992626/range1992626/1
type: Range
persistent identity: range1992626
display id: range1992626
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 1
end: 1230
orientation: inline

SP
type: SequenceAnnotation
persistent identity: annotation1992628
display id: annotation1992628
version: 1
top level: SP-AntiNIP
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157025/annotation1992628/range1992628/1

https://synbiohub.org/public/igem/BBa_K157025/annotation1992628/range1992628/1
type: Range
persistent identity: range1992628
display id: range1992628
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 10
end: 81
orientation: inline

Scar (Thr, Gly)
type: SequenceAnnotation
persistent identity: annotation1992629
display id: annotation1992629
version: 1
top level: SP-AntiNIP
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157025/annotation1992629/range1992629/1

https://synbiohub.org/public/igem/BBa_K157025/annotation1992629/range1992629/1
type: Range
persistent identity: range1992629
display id: range1992629
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 82
end: 87
orientation: inline

Bla2
type: SequenceAnnotation
persistent identity: annotation1992636
display id: annotation1992636
version: 1
top level: SP-AntiNIP
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157025/annotation1992636/range1992636/1

https://synbiohub.org/public/igem/BBa_K157025/annotation1992636/range1992636/1
type: Range
persistent identity: range1992636
display id: range1992636
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 955
end: 1224
orientation: inline

Scar (Thr, Gly)
type: SequenceAnnotation
persistent identity: annotation1992631
display id: annotation1992631
version: 1
top level: SP-AntiNIP
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157025/annotation1992631/range1992631/1

https://synbiohub.org/public/igem/BBa_K157025/annotation1992631/range1992631/1
type: Range
persistent identity: range1992631
display id: range1992631
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 823
end: 828
orientation: inline

Scar (Thr, Gly)
type: SequenceAnnotation
persistent identity: annotation1992635
display id: annotation1992635
version: 1
top level: SP-AntiNIP
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157025/annotation1992635/range1992635/1

https://synbiohub.org/public/igem/BBa_K157025/annotation1992635/range1992635/1
type: Range
persistent identity: range1992635
display id: range1992635
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 949
end: 954
orientation: inline

GGGS-Linker
type: SequenceAnnotation
persistent identity: annotation1992632
display id: annotation1992632
version: 1
top level: SP-AntiNIP
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157025/annotation1992632/range1992632/1

https://synbiohub.org/public/igem/BBa_K157025/annotation1992632/range1992632/1
type: Range
persistent identity: range1992632
display id: range1992632
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 829
end: 873
orientation: inline

NgoMI
type: SequenceAnnotation
persistent identity: annotation1992627
display id: annotation1992627
version: 1
top level: SP-AntiNIP
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157025/annotation1992627/range1992627/1

https://synbiohub.org/public/igem/BBa_K157025/annotation1992627/range1992627/1
type: Range
persistent identity: range1992627
display id: range1992627
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 4
end: 9
orientation: inline

Scar (Thr, Gly)
type: SequenceAnnotation
persistent identity: annotation1992633
display id: annotation1992633
version: 1
top level: SP-AntiNIP
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157025/annotation1992633/range1992633/1

https://synbiohub.org/public/igem/BBa_K157025/annotation1992633/range1992633/1
type: Range
persistent identity: range1992633
display id: range1992633
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 874
end: 879
orientation: inline

BBa_K157003
type: ComponentDefinition
persistent identity: BBa K157003
display id: BBa_K157003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Anti-NIP singlechain Fv-Fragment
creator: iGEM Team Freiburg 2008
created: 2008-10-25T11:00:00Z
modified: 2015-05-08T01:10:54Z
discontinued: false
dominant: false
experience: experience/None
group u list: _232_
m user id: 0
owner id: 1673
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Singlechain Fv.Fragment of an Anti-Nitro-Iodo-Phenol-antibody B1-8. Binds the hapten NIP (Nitro-Iodo-Phenole) with an affinity of 2.0 uM[1,2]. Designed for fusion to proteins or peptides via in frame cloning.
mutable notes: Between BioBrick 1.0 sites this part is flanked 5' by NgoMI(=NgoMIV) and 3' AgeI(=PinAI) to facilitate in frame cloning for protein fusions.
mutable provenance: Gene synthesis by GeneArt, optimized for expression in homo sapiens.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: Anti-NIP sc Fv-fragment
sequence: https://synbiohub.org/public/igem/BBa_K157003_sequence/1

Anti-NIP sc Fv-fragment
type: SequenceAnnotation
persistent identity: annotation2064690
display id: annotation2064690
version: 1
top level: BBa_K157003
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K157003/annotation2064690/range2064690/1

https://synbiohub.org/public/igem/BBa_K157003/annotation2064690/range2064690/1
type: Range
persistent identity: range2064690
display id: range2064690
version: 1
direction: direction/forward
top level: BBa_K157003
start: 1
end: 735
orientation: inline

BBa_J70620
type: ComponentDefinition
persistent identity: BBa J70620
display id: BBa_J70620
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RFC12 Maltose Binding Protein Internal Domain [malE]
creator: Joseph Lynch
created: 2010-08-04T11:00:00Z
modified: 2015-05-08T01:08:25Z
discontinued: false
dominant: false
group u list: _41_
m user id: 0
owner id: 6384
owning group id: 41
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This Maltose-binding protein is designed for either N-terminal or C-terminal fusions. MBP fused proteins can be purified in one step affinity chromatography using a cross-linked amylose matrix and then eluted with 10 mM maltose. Note that the amylose matrix can only be regenerated, in most cases, up to five times. The large size of this tag can also affect the fused protein. MBP fusions often have higher levels of expression and solubility that the native untagged protein, with typical yields of up to 40 mg/liter culture.
mutable notes: Primers:
mutable provenance: pMAL-c2x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000324
role: partType/Tag
sequence annotation: MBP
sequence annotation: Asparagine Spacer
sequence: https://synbiohub.org/public/igem/BBa_J70620_sequence/1

MBP
type: SequenceAnnotation
persistent identity: annotation2076870
display id: annotation2076870
version: 1
top level: BBa_J70620
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J70620/annotation2076870/range2076870/1

https://synbiohub.org/public/igem/BBa_J70620/annotation2076870/range2076870/1
type: Range
persistent identity: range2076870
display id: range2076870
version: 1
direction: direction/forward
top level: BBa_J70620
start: 1
end: 1093
orientation: inline

Asparagine Spacer
type: SequenceAnnotation
persistent identity: annotation2076894
display id: annotation2076894
version: 1
top level: BBa_J70620
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_J70620/annotation2076894/range2076894/1

https://synbiohub.org/public/igem/BBa_J70620/annotation2076894/range2076894/1
type: Range
persistent identity: range2076894
display id: range2076894
version: 1
direction: direction/forward
top level: BBa_J70620
start: 1094
end: 1138
orientation: inline

SgsE | Red
type: ComponentDefinition
persistent identity: BBa K525312
display id: BBa_K525312
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Fusion Protein of S-Layer SgsE and Oxidoreductase
creator: Anna Drong
created: 2011-09-10T11:00:00Z
modified: 2015-05-08T01:12:34Z
discontinued: false
dominant: false
experience: experience/None
group u list: _690_
m user id: 0
owner id: 8543
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: Fusion Protein of S-Layer SgsE and Oxidoreductase;
mutable notes: Assembled
mutable provenance: BBa_K525303, BBa_K525499
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: NgoMIV
sequence annotation: lac operator
sequence annotation: SgsE
sequence annotation: Reductase
sequence annotation: T7 promoter
sequence annotation: B0034
sequence annotation: RBS
sequence annotation: Freiburg scar
sequence annotation: AgeI
sequence annotation: K525499
sequence annotation: K525301
sequence: https://synbiohub.org/public/igem/BBa_K525312_sequence/1

NgoMIV
type: SequenceAnnotation
persistent identity: annotation2136759
display id: annotation2136759
version: 1
top level: SgsE | Red
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K525312/annotation2136759/range2136759/1

https://synbiohub.org/public/igem/BBa_K525312/annotation2136759/range2136759/1
type: Range
persistent identity: range2136759
display id: range2136759
version: 1
direction: direction/forward
top level: SgsE | Red
start: 76
end: 81
orientation: inline

lac operator
type: SequenceAnnotation
persistent identity: annotation2136754
display id: annotation2136754
version: 1
top level: SgsE | Red
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K525312/annotation2136754/range2136754/1

https://synbiohub.org/public/igem/BBa_K525312/annotation2136754/range2136754/1
type: Range
persistent identity: range2136754
display id: range2136754
version: 1
direction: direction/forward
top level: SgsE | Red
start: 20
end: 44
orientation: inline

SgsE
type: SequenceAnnotation
persistent identity: annotation2136757
display id: annotation2136757
version: 1
top level: SgsE | Red
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K525312/annotation2136757/range2136757/1

https://synbiohub.org/public/igem/BBa_K525312/annotation2136757/range2136757/1
type: Range
persistent identity: range2136757
display id: range2136757
version: 1
direction: direction/forward
top level: SgsE | Red
start: 73
end: 2400
orientation: inline

Reductase
type: SequenceAnnotation
persistent identity: annotation2136762
display id: annotation2136762
version: 1
top level: SgsE | Red
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K525312/annotation2136762/range2136762/1

https://synbiohub.org/public/igem/BBa_K525312/annotation2136762/range2136762/1
type: Range
persistent identity: range2136762
display id: range2136762
version: 1
direction: direction/forward
top level: SgsE | Red
start: 2407
end: 3156
orientation: inline

T7 promoter
type: SequenceAnnotation
persistent identity: annotation2136753
display id: annotation2136753
version: 1
top level: SgsE | Red
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K525312/annotation2136753/range2136753/1

https://synbiohub.org/public/igem/BBa_K525312/annotation2136753/range2136753/1
type: Range
persistent identity: range2136753
display id: range2136753
version: 1
direction: direction/forward
top level: SgsE | Red
start: 1
end: 20
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation2136755
display id: annotation2136755
version: 1
top level: SgsE | Red
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K525312/annotation2136755/range2136755/1

https://synbiohub.org/public/igem/BBa_K525312/annotation2136755/range2136755/1
type: Range
persistent identity: range2136755
display id: range2136755
version: 1
direction: direction/forward
top level: SgsE | Red
start: 53
end: 64
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2136756
display id: annotation2136756
version: 1
top level: SgsE | Red
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K525312/annotation2136756/range2136756/1

https://synbiohub.org/public/igem/BBa_K525312/annotation2136756/range2136756/1
type: Range
persistent identity: range2136756
display id: range2136756
version: 1
direction: direction/forward
top level: SgsE | Red
start: 56
end: 59
orientation: inline

Freiburg scar
type: SequenceAnnotation
persistent identity: annotation2136760
display id: annotation2136760
version: 1
top level: SgsE | Red
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K525312/annotation2136760/range2136760/1

https://synbiohub.org/public/igem/BBa_K525312/annotation2136760/range2136760/1
type: Range
persistent identity: range2136760
display id: range2136760
version: 1
direction: direction/forward
top level: SgsE | Red
start: 2401
end: 2406
orientation: inline

AgeI
type: SequenceAnnotation
persistent identity: annotation2136763
display id: annotation2136763
version: 1
top level: SgsE | Red
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K525312/annotation2136763/range2136763/1

https://synbiohub.org/public/igem/BBa_K525312/annotation2136763/range2136763/1
type: Range
persistent identity: range2136763
display id: range2136763
version: 1
direction: direction/forward
top level: SgsE | Red
start: 3148
end: 3152
orientation: inline

K525499
type: SequenceAnnotation
persistent identity: annotation2136761
display id: annotation2136761
version: 1
top level: SgsE | Red
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K525312/annotation2136761/range2136761/1

https://synbiohub.org/public/igem/BBa_K525312/annotation2136761/range2136761/1
type: Range
persistent identity: range2136761
display id: range2136761
version: 1
direction: direction/forward
top level: SgsE | Red
start: 2407
end: 3156
orientation: inline

K525301
type: SequenceAnnotation
persistent identity: annotation2136758
display id: annotation2136758
version: 1
top level: SgsE | Red
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K525312/annotation2136758/range2136758/1

https://synbiohub.org/public/igem/BBa_K525312/annotation2136758/range2136758/1
type: Range
persistent identity: range2136758
display id: range2136758
version: 1
direction: direction/forward
top level: SgsE | Red
start: 73
end: 2400
orientation: inline

SbpA | Luc
type: ComponentDefinition
persistent identity: BBa K525411
display id: BBa_K525411
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Fusion Protein of S-Layer SbpA and Firefly-Luciferase
creator: Anna Drong
created: 2011-09-12T11:00:00Z
modified: 2015-05-08T01:12:34Z
discontinued: false
dominant: false
experience: experience/None
group u list: _690_
m user id: 0
owner id: 8543
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: PT7 + RBS + SpbA || Luc
mutable notes: Assembled
mutable provenance: BBa_K525403, BBa_K525999
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: RBS
sequence annotation: TAA
sequence annotation: lac operator
sequence annotation: K525999
sequence annotation: SbpA domain
sequence annotation: NgoMIV
sequence annotation: ATG
sequence annotation: B0034
sequence annotation: Freiburg scar
sequence annotation: T7 promoter
sequence annotation: Luciferase
sequence annotation: K525401
sequence annotation: AgeI
sequence: https://synbiohub.org/public/igem/BBa_K525411_sequence/1

RBS
type: SequenceAnnotation
persistent identity: annotation2161270
display id: annotation2161270
version: 1
top level: SbpA | Luc
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K525411/annotation2161270/range2161270/1

https://synbiohub.org/public/igem/BBa_K525411/annotation2161270/range2161270/1
type: Range
persistent identity: range2161270
display id: range2161270
version: 1
direction: direction/forward
top level: SbpA | Luc
start: 56
end: 59
orientation: inline

TAA
type: SequenceAnnotation
persistent identity: annotation2161278
display id: annotation2161278
version: 1
top level: SbpA | Luc
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K525411/annotation2161278/range2161278/1

https://synbiohub.org/public/igem/BBa_K525411/annotation2161278/range2161278/1
type: Range
persistent identity: range2161278
display id: range2161278
version: 1
direction: direction/forward
top level: SbpA | Luc
start: 4852
end: 4854
orientation: inline

lac operator
type: SequenceAnnotation
persistent identity: annotation2161268
display id: annotation2161268
version: 1
top level: SbpA | Luc
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K525411/annotation2161268/range2161268/1

https://synbiohub.org/public/igem/BBa_K525411/annotation2161268/range2161268/1
type: Range
persistent identity: range2161268
display id: range2161268
version: 1
direction: direction/forward
top level: SbpA | Luc
start: 20
end: 44
orientation: inline

K525999
type: SequenceAnnotation
persistent identity: annotation2161276
display id: annotation2161276
version: 1
top level: SbpA | Luc
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K525411/annotation2161276/range2161276/1

https://synbiohub.org/public/igem/BBa_K525411/annotation2161276/range2161276/1
type: Range
persistent identity: range2161276
display id: range2161276
version: 1
direction: direction/forward
top level: SbpA | Luc
start: 3199
end: 4854
orientation: inline

SbpA domain
type: SequenceAnnotation
persistent identity: annotation2161272
display id: annotation2161272
version: 1
top level: SbpA | Luc
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K525411/annotation2161272/range2161272/1

https://synbiohub.org/public/igem/BBa_K525411/annotation2161272/range2161272/1
type: Range
persistent identity: range2161272
display id: range2161272
version: 1
direction: direction/forward
top level: SbpA | Luc
start: 73
end: 3192
orientation: inline

NgoMIV
type: SequenceAnnotation
persistent identity: annotation2161279
display id: annotation2161279
version: 1
top level: SbpA | Luc
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K525411/annotation2161279/range2161279/1

https://synbiohub.org/public/igem/BBa_K525411/annotation2161279/range2161279/1
type: Range
persistent identity: range2161279
display id: range2161279
version: 1
direction: direction/forward
top level: SbpA | Luc
start: 76
end: 81
orientation: inline

ATG
type: SequenceAnnotation
persistent identity: annotation2161273
display id: annotation2161273
version: 1
top level: SbpA | Luc
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K525411/annotation2161273/range2161273/1

https://synbiohub.org/public/igem/BBa_K525411/annotation2161273/range2161273/1
type: Range
persistent identity: range2161273
display id: range2161273
version: 1
direction: direction/forward
top level: SbpA | Luc
start: 73
end: 75
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation2161269
display id: annotation2161269
version: 1
top level: SbpA | Luc
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K525411/annotation2161269/range2161269/1

https://synbiohub.org/public/igem/BBa_K525411/annotation2161269/range2161269/1
type: Range
persistent identity: range2161269
display id: range2161269
version: 1
direction: direction/forward
top level: SbpA | Luc
start: 53
end: 64
orientation: inline

Freiburg scar
type: SequenceAnnotation
persistent identity: annotation2161274
display id: annotation2161274
version: 1
top level: SbpA | Luc
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K525411/annotation2161274/range2161274/1

https://synbiohub.org/public/igem/BBa_K525411/annotation2161274/range2161274/1
type: Range
persistent identity: range2161274
display id: range2161274
version: 1
direction: direction/forward
top level: SbpA | Luc
start: 3193
end: 3198
orientation: inline

T7 promoter
type: SequenceAnnotation
persistent identity: annotation2161267
display id: annotation2161267
version: 1
top level: SbpA | Luc
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K525411/annotation2161267/range2161267/1

https://synbiohub.org/public/igem/BBa_K525411/annotation2161267/range2161267/1
type: Range
persistent identity: range2161267
display id: range2161267
version: 1
direction: direction/forward
top level: SbpA | Luc
start: 1
end: 20
orientation: inline

Luciferase
type: SequenceAnnotation
persistent identity: annotation2161275
display id: annotation2161275
version: 1
top level: SbpA | Luc
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K525411/annotation2161275/range2161275/1

https://synbiohub.org/public/igem/BBa_K525411/annotation2161275/range2161275/1
type: Range
persistent identity: range2161275
display id: range2161275
version: 1
direction: direction/forward
top level: SbpA | Luc
start: 3199
end: 4854
orientation: inline

K525401
type: SequenceAnnotation
persistent identity: annotation2161271
display id: annotation2161271
version: 1
top level: SbpA | Luc
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K525411/annotation2161271/range2161271/1

https://synbiohub.org/public/igem/BBa_K525411/annotation2161271/range2161271/1
type: Range
persistent identity: range2161271
display id: range2161271
version: 1
direction: direction/forward
top level: SbpA | Luc
start: 73
end: 3192
orientation: inline

AgeI
type: SequenceAnnotation
persistent identity: annotation2161277
display id: annotation2161277
version: 1
top level: SbpA | Luc
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K525411/annotation2161277/range2161277/1

https://synbiohub.org/public/igem/BBa_K525411/annotation2161277/range2161277/1
type: Range
persistent identity: range2161277
display id: range2161277
version: 1
direction: direction/forward
top level: SbpA | Luc
start: 4846
end: 4851
orientation: inline

SbpA
type: ComponentDefinition
persistent identity: BBa K525403
display id: BBa_K525403
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: S-Layer SbpA from Lysinibacillus sphaericus with T7/lac
creator: Timo Wolf
created: 2011-09-09T11:00:00Z
modified: 2015-05-08T01:12:34Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _690_
m user id: 0
owner id: 6668
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: S-Layer SgsE from Geobacillus stearothermophilus with T7/lac
mutable notes: Synthesis
mutable provenance: Synthesis
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: AgeI
sequence annotation: TAA
sequence annotation: ATG
sequence annotation: NgoMIV
sequence annotation: B0034
sequence annotation: RBS
sequence annotation: K525401
sequence annotation: T7 promoter
sequence annotation: SbpA domain
sequence annotation: lac operator
sequence: https://synbiohub.org/public/igem/BBa_K525403_sequence/1

AgeI
type: SequenceAnnotation
persistent identity: annotation2129903
display id: annotation2129903
version: 1
top level: SbpA
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K525403/annotation2129903/range2129903/1

https://synbiohub.org/public/igem/BBa_K525403/annotation2129903/range2129903/1
type: Range
persistent identity: range2129903
display id: range2129903
version: 1
direction: direction/forward
top level: SbpA
start: 3193
end: 3198
orientation: inline

TAA
type: SequenceAnnotation
persistent identity: annotation2129901
display id: annotation2129901
version: 1
top level: SbpA
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K525403/annotation2129901/range2129901/1

https://synbiohub.org/public/igem/BBa_K525403/annotation2129901/range2129901/1
type: Range
persistent identity: range2129901
display id: range2129901
version: 1
direction: direction/forward
top level: SbpA
start: 3199
end: 3201
orientation: inline

ATG
type: SequenceAnnotation
persistent identity: annotation2129900
display id: annotation2129900
version: 1
top level: SbpA
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K525403/annotation2129900/range2129900/1

https://synbiohub.org/public/igem/BBa_K525403/annotation2129900/range2129900/1
type: Range
persistent identity: range2129900
display id: range2129900
version: 1
direction: direction/forward
top level: SbpA
start: 73
end: 75
orientation: inline

NgoMIV
type: SequenceAnnotation
persistent identity: annotation2129902
display id: annotation2129902
version: 1
top level: SbpA
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K525403/annotation2129902/range2129902/1

https://synbiohub.org/public/igem/BBa_K525403/annotation2129902/range2129902/1
type: Range
persistent identity: range2129902
display id: range2129902
version: 1
direction: direction/forward
top level: SbpA
start: 76
end: 81
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation2129898
display id: annotation2129898
version: 1
top level: SbpA
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K525403/annotation2129898/range2129898/1

https://synbiohub.org/public/igem/BBa_K525403/annotation2129898/range2129898/1
type: Range
persistent identity: range2129898
display id: range2129898
version: 1
direction: direction/forward
top level: SbpA
start: 53
end: 64
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2129899
display id: annotation2129899
version: 1
top level: SbpA
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K525403/annotation2129899/range2129899/1

https://synbiohub.org/public/igem/BBa_K525403/annotation2129899/range2129899/1
type: Range
persistent identity: range2129899
display id: range2129899
version: 1
direction: direction/forward
top level: SbpA
start: 56
end: 59
orientation: inline

K525401
type: SequenceAnnotation
persistent identity: annotation2129801
display id: annotation2129801
version: 1
top level: SbpA
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K525403/annotation2129801/range2129801/1

https://synbiohub.org/public/igem/BBa_K525403/annotation2129801/range2129801/1
type: Range
persistent identity: range2129801
display id: range2129801
version: 1
direction: direction/forward
top level: SbpA
start: 73
end: 3201
orientation: inline

T7 promoter
type: SequenceAnnotation
persistent identity: annotation2129896
display id: annotation2129896
version: 1
top level: SbpA
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K525403/annotation2129896/range2129896/1

https://synbiohub.org/public/igem/BBa_K525403/annotation2129896/range2129896/1
type: Range
persistent identity: range2129896
display id: range2129896
version: 1
direction: direction/forward
top level: SbpA
start: 1
end: 20
orientation: inline

SbpA domain
type: SequenceAnnotation
persistent identity: annotation2129904
display id: annotation2129904
version: 1
top level: SbpA
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K525403/annotation2129904/range2129904/1

https://synbiohub.org/public/igem/BBa_K525403/annotation2129904/range2129904/1
type: Range
persistent identity: range2129904
display id: range2129904
version: 1
direction: direction/forward
top level: SbpA
start: 73
end: 3201
orientation: inline

lac operator
type: SequenceAnnotation
persistent identity: annotation2129897
display id: annotation2129897
version: 1
top level: SbpA
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K525403/annotation2129897/range2129897/1

https://synbiohub.org/public/igem/BBa_K525403/annotation2129897/range2129897/1
type: Range
persistent identity: range2129897
display id: range2129897
version: 1
direction: direction/forward
top level: SbpA
start: 20
end: 44
orientation: inline

SP-AntiNIP
type: ComponentDefinition
persistent identity: BBa K157027
display id: BBa_K157027
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: SP-AntiNIP-GGGSlinker-Tm-nYFP
creator: iGEM Team Freiburg 2008
created: 2008-10-25T11:00:00Z
modified: 2015-05-08T01:10:55Z
discontinued: false
dominant: false
experience: experience/None
group u list: _232_
m user id: 0
owner id: 1673
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Part consists of the signal peptide of Erbb1/EGF-receptor, which ensures the transport to the cell membrane scFV-antiNIP, GGGSflexlinker (extracellular parts), the trans-membrane region of the Erbb1/EGF-receptor and the N-terminal part of the split-fluorophor YFP(intracellular part).
mutable notes: none
mutable provenance: Derived by cloning of the parts BBa_K157001, BBa_K157003, BBa_K157013, BBa_K157002, BBa_K157008. For further information see the description of the parts mentioned above.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: Scar (Thr, Gly)
sequence annotation: SP
sequence annotation: N-Term_YFP
sequence annotation: GGGS-Linker
sequence annotation: NgoMI
sequence annotation: SP-AntiNIP-GGGSlinker-Tm-nYFP
sequence annotation: Tm
sequence annotation: AgeI
sequence annotation: Sc-Fv_anti-NIP
sequence: https://synbiohub.org/public/igem/BBa_K157027_sequence/1

Scar (Thr, Gly)
type: SequenceAnnotation
persistent identity: annotation1992650
display id: annotation1992650
version: 1
top level: SP-AntiNIP
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157027/annotation1992650/range1992650/1

https://synbiohub.org/public/igem/BBa_K157027/annotation1992650/range1992650/1
type: Range
persistent identity: range1992650
display id: range1992650
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 82
end: 87
orientation: inline

Scar (Thr, Gly)
type: SequenceAnnotation
persistent identity: annotation1992658
display id: annotation1992658
version: 1
top level: SP-AntiNIP
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157027/annotation1992658/range1992658/1

https://synbiohub.org/public/igem/BBa_K157027/annotation1992658/range1992658/1
type: Range
persistent identity: range1992658
display id: range1992658
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 874
end: 879
orientation: inline

Scar (Thr, Gly)
type: SequenceAnnotation
persistent identity: annotation1992659
display id: annotation1992659
version: 1
top level: SP-AntiNIP
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157027/annotation1992659/range1992659/1

https://synbiohub.org/public/igem/BBa_K157027/annotation1992659/range1992659/1
type: Range
persistent identity: range1992659
display id: range1992659
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 949
end: 954
orientation: inline

SP
type: SequenceAnnotation
persistent identity: annotation1992649
display id: annotation1992649
version: 1
top level: SP-AntiNIP
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157027/annotation1992649/range1992649/1

https://synbiohub.org/public/igem/BBa_K157027/annotation1992649/range1992649/1
type: Range
persistent identity: range1992649
display id: range1992649
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 10
end: 81
orientation: inline

N-Term_YFP
type: SequenceAnnotation
persistent identity: annotation1992660
display id: annotation1992660
version: 1
top level: SP-AntiNIP
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157027/annotation1992660/range1992660/1

https://synbiohub.org/public/igem/BBa_K157027/annotation1992660/range1992660/1
type: Range
persistent identity: range1992660
display id: range1992660
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 955
end: 1422
orientation: inline

GGGS-Linker
type: SequenceAnnotation
persistent identity: annotation1992653
display id: annotation1992653
version: 1
top level: SP-AntiNIP
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157027/annotation1992653/range1992653/1

https://synbiohub.org/public/igem/BBa_K157027/annotation1992653/range1992653/1
type: Range
persistent identity: range1992653
display id: range1992653
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 829
end: 873
orientation: inline

NgoMI
type: SequenceAnnotation
persistent identity: annotation1990177
display id: annotation1990177
version: 1
top level: SP-AntiNIP
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157027/annotation1990177/range1990177/1

https://synbiohub.org/public/igem/BBa_K157027/annotation1990177/range1990177/1
type: Range
persistent identity: range1990177
display id: range1990177
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 4
end: 9
orientation: inline

SP-AntiNIP-GGGSlinker-Tm-nYFP
type: SequenceAnnotation
persistent identity: annotation1990179
display id: annotation1990179
version: 1
top level: SP-AntiNIP
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K157027/annotation1990179/range1990179/1

https://synbiohub.org/public/igem/BBa_K157027/annotation1990179/range1990179/1
type: Range
persistent identity: range1990179
display id: range1990179
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 1
end: 1428
orientation: inline

Tm
type: SequenceAnnotation
persistent identity: annotation1992655
display id: annotation1992655
version: 1
top level: SP-AntiNIP
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157027/annotation1992655/range1992655/1

https://synbiohub.org/public/igem/BBa_K157027/annotation1992655/range1992655/1
type: Range
persistent identity: range1992655
display id: range1992655
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 880
end: 948
orientation: inline

AgeI
type: SequenceAnnotation
persistent identity: annotation1990178
display id: annotation1990178
version: 1
top level: SP-AntiNIP
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157027/annotation1990178/range1990178/1

https://synbiohub.org/public/igem/BBa_K157027/annotation1990178/range1990178/1
type: Range
persistent identity: range1990178
display id: range1990178
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 1423
end: 1428
orientation: inline

Scar (Thr, Gly)
type: SequenceAnnotation
persistent identity: annotation1992652
display id: annotation1992652
version: 1
top level: SP-AntiNIP
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157027/annotation1992652/range1992652/1

https://synbiohub.org/public/igem/BBa_K157027/annotation1992652/range1992652/1
type: Range
persistent identity: range1992652
display id: range1992652
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 823
end: 828
orientation: inline

Sc-Fv_anti-NIP
type: SequenceAnnotation
persistent identity: annotation1992651
display id: annotation1992651
version: 1
top level: SP-AntiNIP
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157027/annotation1992651/range1992651/1

https://synbiohub.org/public/igem/BBa_K157027/annotation1992651/range1992651/1
type: Range
persistent identity: range1992651
display id: range1992651
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 88
end: 822
orientation: inline

BBa_K525008
type: ComponentDefinition
persistent identity: BBa K525008
display id: BBa_K525008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Fusion protein of S-Layer SbpA with T7/lac and BisdA and BisdB
creator: Anna Drong
created: 2011-09-11T11:00:00Z
modified: 2015-05-08T01:12:34Z
discontinued: false
dominant: false
experience: experience/None
group u list: _690_
m user id: 0
owner id: 8543
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: PT7 + RBS + SbpA || BisdA || BisdB
mutable notes: Assembled
mutable provenance: BBa_K525403 , BBa_K123000 , BBa_K123001
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: NgoMIV
sequence annotation: BisdB
sequence annotation: B0034
sequence annotation: ATG
sequence annotation: AgeI
sequence annotation: T7
sequence annotation: Freiburg Scar
sequence annotation: TAA
sequence annotation: K525401
sequence annotation: K525515
sequence annotation: lac operator
sequence annotation: BisdA
sequence annotation: SbpA domain
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_K525008_sequence/1

NgoMIV
type: SequenceAnnotation
persistent identity: annotation2161201
display id: annotation2161201
version: 1
top level: BBa_K525008
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K525008/annotation2161201/range2161201/1

https://synbiohub.org/public/igem/BBa_K525008/annotation2161201/range2161201/1
type: Range
persistent identity: range2161201
display id: range2161201
version: 1
direction: direction/forward
top level: BBa_K525008
start: 76
end: 82
orientation: inline

BisdB
type: SequenceAnnotation
persistent identity: annotation2161205
display id: annotation2161205
version: 1
top level: BBa_K525008
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K525008/annotation2161205/range2161205/1

https://synbiohub.org/public/igem/BBa_K525008/annotation2161205/range2161205/1
type: Range
persistent identity: range2161205
display id: range2161205
version: 1
direction: direction/forward
top level: BBa_K525008
start: 3517
end: 4791
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation2161196
display id: annotation2161196
version: 1
top level: BBa_K525008
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K525008/annotation2161196/range2161196/1

https://synbiohub.org/public/igem/BBa_K525008/annotation2161196/range2161196/1
type: Range
persistent identity: range2161196
display id: range2161196
version: 1
direction: direction/forward
top level: BBa_K525008
start: 53
end: 64
orientation: inline

ATG
type: SequenceAnnotation
persistent identity: annotation2161199
display id: annotation2161199
version: 1
top level: BBa_K525008
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K525008/annotation2161199/range2161199/1

https://synbiohub.org/public/igem/BBa_K525008/annotation2161199/range2161199/1
type: Range
persistent identity: range2161199
display id: range2161199
version: 1
direction: direction/forward
top level: BBa_K525008
start: 73
end: 75
orientation: inline

AgeI
type: SequenceAnnotation
persistent identity: annotation2161208
display id: annotation2161208
version: 1
top level: BBa_K525008
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K525008/annotation2161208/range2161208/1

https://synbiohub.org/public/igem/BBa_K525008/annotation2161208/range2161208/1
type: Range
persistent identity: range2161208
display id: range2161208
version: 1
direction: direction/forward
top level: BBa_K525008
start: 4783
end: 4788
orientation: inline

T7
type: SequenceAnnotation
persistent identity: annotation2161194
display id: annotation2161194
version: 1
top level: BBa_K525008
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K525008/annotation2161194/range2161194/1

https://synbiohub.org/public/igem/BBa_K525008/annotation2161194/range2161194/1
type: Range
persistent identity: range2161194
display id: range2161194
version: 1
direction: direction/forward
top level: BBa_K525008
start: 1
end: 20
orientation: inline

Freiburg Scar
type: SequenceAnnotation
persistent identity: annotation2161202
display id: annotation2161202
version: 1
top level: BBa_K525008
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K525008/annotation2161202/range2161202/1

https://synbiohub.org/public/igem/BBa_K525008/annotation2161202/range2161202/1
type: Range
persistent identity: range2161202
display id: range2161202
version: 1
direction: direction/forward
top level: BBa_K525008
start: 3193
end: 3198
orientation: inline

TAA
type: SequenceAnnotation
persistent identity: annotation2161206
display id: annotation2161206
version: 1
top level: BBa_K525008
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K525008/annotation2161206/range2161206/1

https://synbiohub.org/public/igem/BBa_K525008/annotation2161206/range2161206/1
type: Range
persistent identity: range2161206
display id: range2161206
version: 1
direction: direction/forward
top level: BBa_K525008
start: 4789
end: 4791
orientation: inline

K525401
type: SequenceAnnotation
persistent identity: annotation2161198
display id: annotation2161198
version: 1
top level: BBa_K525008
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K525008/annotation2161198/range2161198/1

https://synbiohub.org/public/igem/BBa_K525008/annotation2161198/range2161198/1
type: Range
persistent identity: range2161198
display id: range2161198
version: 1
direction: direction/forward
top level: BBa_K525008
start: 73
end: 3192
orientation: inline

K525515
type: SequenceAnnotation
persistent identity: annotation2161207
display id: annotation2161207
version: 1
top level: BBa_K525008
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K525008/annotation2161207/range2161207/1

https://synbiohub.org/public/igem/BBa_K525008/annotation2161207/range2161207/1
type: Range
persistent identity: range2161207
display id: range2161207
version: 1
direction: direction/forward
top level: BBa_K525008
start: 3199
end: 4791
orientation: inline

lac operator
type: SequenceAnnotation
persistent identity: annotation2161195
display id: annotation2161195
version: 1
top level: BBa_K525008
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K525008/annotation2161195/range2161195/1

https://synbiohub.org/public/igem/BBa_K525008/annotation2161195/range2161195/1
type: Range
persistent identity: range2161195
display id: range2161195
version: 1
direction: direction/forward
top level: BBa_K525008
start: 20
end: 44
orientation: inline

BisdA
type: SequenceAnnotation
persistent identity: annotation2161203
display id: annotation2161203
version: 1
top level: BBa_K525008
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K525008/annotation2161203/range2161203/1

https://synbiohub.org/public/igem/BBa_K525008/annotation2161203/range2161203/1
type: Range
persistent identity: range2161203
display id: range2161203
version: 1
direction: direction/forward
top level: BBa_K525008
start: 3199
end: 3510
orientation: inline

SbpA domain
type: SequenceAnnotation
persistent identity: annotation2161200
display id: annotation2161200
version: 1
top level: BBa_K525008
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K525008/annotation2161200/range2161200/1

https://synbiohub.org/public/igem/BBa_K525008/annotation2161200/range2161200/1
type: Range
persistent identity: range2161200
display id: range2161200
version: 1
direction: direction/forward
top level: BBa_K525008
start: 73
end: 3192
orientation: inline

Freiburg Scar
type: SequenceAnnotation
persistent identity: annotation2161204
display id: annotation2161204
version: 1
top level: BBa_K525008
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K525008/annotation2161204/range2161204/1

https://synbiohub.org/public/igem/BBa_K525008/annotation2161204/range2161204/1
type: Range
persistent identity: range2161204
display id: range2161204
version: 1
direction: direction/forward
top level: BBa_K525008
start: 3511
end: 3516
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2161197
display id: annotation2161197
version: 1
top level: BBa_K525008
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K525008/annotation2161197/range2161197/1

https://synbiohub.org/public/igem/BBa_K525008/annotation2161197/range2161197/1
type: Range
persistent identity: range2161197
display id: range2161197
version: 1
direction: direction/forward
top level: BBa_K525008
start: 56
end: 59
orientation: inline

SP-AntiNIP
type: ComponentDefinition
persistent identity: BBa K157030
display id: BBa_K157030
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: SP-AntiNIP-GGGSlinker-Tm-Splitluciferase2/2
creator: iGEM Team Freiburg 2008
created: 2008-10-25T11:00:00Z
modified: 2015-05-08T01:10:55Z
discontinued: false
dominant: true
experience: experience/None
group u list: _232_
m user id: 0
owner id: 1673
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Part consists of the signal peptide of Erbb1/EGF-receptor, scFV-antiNIP, GGGSflexlinker (extracellular part), the trans-membrane region of the Erbb1/EGF-receptor and the C-terminal part of the split fluorophor YFP with a fluolinker joined to its N-Terminus (intracellular part).
mutable notes: none
mutable provenance: Derived by cloning of the parts BBa_K157001, BBa_K157003, BBa_K157013, BBa_K157002 and Part one of Split- Luciferase For further information see the description of the parts mentioned above.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: Tm
sequence annotation: Scar (Thr, Gly)
sequence annotation: GGGS-Linker
sequence annotation: NgoMI
sequence annotation: SP
sequence annotation: AgeI
sequence annotation: SP-AntiNIP-GGGSlinker-Tm-Splitluciferase2/2
sequence annotation: Splitluciferase2/2
sequence annotation: Sc-Fv_anti-NIP
sequence: https://synbiohub.org/public/igem/BBa_K157030_sequence/1

Tm
type: SequenceAnnotation
persistent identity: annotation1993315
display id: annotation1993315
version: 1
top level: SP-AntiNIP
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157030/annotation1993315/range1993315/1

https://synbiohub.org/public/igem/BBa_K157030/annotation1993315/range1993315/1
type: Range
persistent identity: range1993315
display id: range1993315
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 880
end: 948
orientation: inline

Scar (Thr, Gly)
type: SequenceAnnotation
persistent identity: annotation1993312
display id: annotation1993312
version: 1
top level: SP-AntiNIP
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157030/annotation1993312/range1993312/1

https://synbiohub.org/public/igem/BBa_K157030/annotation1993312/range1993312/1
type: Range
persistent identity: range1993312
display id: range1993312
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 823
end: 828
orientation: inline

GGGS-Linker
type: SequenceAnnotation
persistent identity: annotation1993313
display id: annotation1993313
version: 1
top level: SP-AntiNIP
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157030/annotation1993313/range1993313/1

https://synbiohub.org/public/igem/BBa_K157030/annotation1993313/range1993313/1
type: Range
persistent identity: range1993313
display id: range1993313
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 829
end: 873
orientation: inline

NgoMI
type: SequenceAnnotation
persistent identity: annotation1990263
display id: annotation1990263
version: 1
top level: SP-AntiNIP
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157030/annotation1990263/range1990263/1

https://synbiohub.org/public/igem/BBa_K157030/annotation1990263/range1990263/1
type: Range
persistent identity: range1990263
display id: range1990263
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 4
end: 9
orientation: inline

SP
type: SequenceAnnotation
persistent identity: annotation1993309
display id: annotation1993309
version: 1
top level: SP-AntiNIP
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157030/annotation1993309/range1993309/1

https://synbiohub.org/public/igem/BBa_K157030/annotation1993309/range1993309/1
type: Range
persistent identity: range1993309
display id: range1993309
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 10
end: 81
orientation: inline

Scar (Thr, Gly)
type: SequenceAnnotation
persistent identity: annotation1993316
display id: annotation1993316
version: 1
top level: SP-AntiNIP
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157030/annotation1993316/range1993316/1

https://synbiohub.org/public/igem/BBa_K157030/annotation1993316/range1993316/1
type: Range
persistent identity: range1993316
display id: range1993316
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 949
end: 954
orientation: inline

AgeI
type: SequenceAnnotation
persistent identity: annotation1990264
display id: annotation1990264
version: 1
top level: SP-AntiNIP
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157030/annotation1990264/range1990264/1

https://synbiohub.org/public/igem/BBa_K157030/annotation1990264/range1990264/1
type: Range
persistent identity: range1990264
display id: range1990264
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 1231
end: 1236
orientation: inline

SP-AntiNIP-GGGSlinker-Tm-Splitluciferase2/2
type: SequenceAnnotation
persistent identity: annotation1990265
display id: annotation1990265
version: 1
top level: SP-AntiNIP
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K157030/annotation1990265/range1990265/1

https://synbiohub.org/public/igem/BBa_K157030/annotation1990265/range1990265/1
type: Range
persistent identity: range1990265
display id: range1990265
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 1
end: 1236
orientation: inline

Scar (Thr, Gly)
type: SequenceAnnotation
persistent identity: annotation1993310
display id: annotation1993310
version: 1
top level: SP-AntiNIP
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157030/annotation1993310/range1993310/1

https://synbiohub.org/public/igem/BBa_K157030/annotation1993310/range1993310/1
type: Range
persistent identity: range1993310
display id: range1993310
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 82
end: 87
orientation: inline

Splitluciferase2/2
type: SequenceAnnotation
persistent identity: annotation1993317
display id: annotation1993317
version: 1
top level: SP-AntiNIP
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157030/annotation1993317/range1993317/1

https://synbiohub.org/public/igem/BBa_K157030/annotation1993317/range1993317/1
type: Range
persistent identity: range1993317
display id: range1993317
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 955
end: 1230
orientation: inline

Sc-Fv_anti-NIP
type: SequenceAnnotation
persistent identity: annotation1993311
display id: annotation1993311
version: 1
top level: SP-AntiNIP
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157030/annotation1993311/range1993311/1

https://synbiohub.org/public/igem/BBa_K157030/annotation1993311/range1993311/1
type: Range
persistent identity: range1993311
display id: range1993311
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 88
end: 822
orientation: inline

Scar (Thr, Gly)
type: SequenceAnnotation
persistent identity: annotation1993314
display id: annotation1993314
version: 1
top level: SP-AntiNIP
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157030/annotation1993314/range1993314/1

https://synbiohub.org/public/igem/BBa_K157030/annotation1993314/range1993314/1
type: Range
persistent identity: range1993314
display id: range1993314
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 874
end: 879
orientation: inline

Tm-Splitlu
type: ComponentDefinition
persistent identity: BBa K157021
display id: BBa_K157021
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Tm-Splitluciferase2/2
creator: iGEM Team Freiburg 2008
created: 2008-10-25T11:00:00Z
modified: 2015-05-08T01:10:54Z
discontinued: false
dominant: false
experience: experience/None
group u list: _232_
m user id: 0
owner id: 1673
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Second part of Split-Luciferase fused to the trans-membrane region of the Erbb1/EGF-receptor.
mutable notes: none
mutable provenance: Derived by cloning of the parts BBa_K157002 and second part of Split-Luciferase. For further information see the description of the parts mentioned above.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: Tm
sequence annotation: Split-Luciferase2
sequence annotation: NgoMI
sequence annotation: Scar (Thr, Gly)
sequence annotation: Tm-Split-Luciferase2/2
sequence annotation: AgeI
sequence: https://synbiohub.org/public/igem/BBa_K157021_sequence/1

Tm
type: SequenceAnnotation
persistent identity: annotation1991543
display id: annotation1991543
version: 1
top level: Tm-Splitlu
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157021/annotation1991543/range1991543/1

https://synbiohub.org/public/igem/BBa_K157021/annotation1991543/range1991543/1
type: Range
persistent identity: range1991543
display id: range1991543
version: 1
direction: direction/forward
top level: Tm-Splitlu
start: 10
end: 78
orientation: inline

Split-Luciferase2
type: SequenceAnnotation
persistent identity: annotation1991545
display id: annotation1991545
version: 1
top level: Tm-Splitlu
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157021/annotation1991545/range1991545/1

https://synbiohub.org/public/igem/BBa_K157021/annotation1991545/range1991545/1
type: Range
persistent identity: range1991545
display id: range1991545
version: 1
direction: direction/forward
top level: Tm-Splitlu
start: 85
end: 360
orientation: inline

NgoMI
type: SequenceAnnotation
persistent identity: annotation1991542
display id: annotation1991542
version: 1
top level: Tm-Splitlu
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157021/annotation1991542/range1991542/1

https://synbiohub.org/public/igem/BBa_K157021/annotation1991542/range1991542/1
type: Range
persistent identity: range1991542
display id: range1991542
version: 1
direction: direction/forward
top level: Tm-Splitlu
start: 4
end: 9
orientation: inline

Scar (Thr, Gly)
type: SequenceAnnotation
persistent identity: annotation1991544
display id: annotation1991544
version: 1
top level: Tm-Splitlu
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157021/annotation1991544/range1991544/1

https://synbiohub.org/public/igem/BBa_K157021/annotation1991544/range1991544/1
type: Range
persistent identity: range1991544
display id: range1991544
version: 1
direction: direction/forward
top level: Tm-Splitlu
start: 79
end: 84
orientation: inline

Tm-Split-Luciferase2/2
type: SequenceAnnotation
persistent identity: annotation1991541
display id: annotation1991541
version: 1
top level: Tm-Splitlu
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K157021/annotation1991541/range1991541/1

https://synbiohub.org/public/igem/BBa_K157021/annotation1991541/range1991541/1
type: Range
persistent identity: range1991541
display id: range1991541
version: 1
direction: direction/forward
top level: Tm-Splitlu
start: 1
end: 366
orientation: inline

AgeI
type: SequenceAnnotation
persistent identity: annotation1991546
display id: annotation1991546
version: 1
top level: Tm-Splitlu
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157021/annotation1991546/range1991546/1

https://synbiohub.org/public/igem/BBa_K157021/annotation1991546/range1991546/1
type: Range
persistent identity: range1991546
display id: range1991546
version: 1
direction: direction/forward
top level: Tm-Splitlu
start: 361
end: 366
orientation: inline

BisdAB
type: ComponentDefinition
persistent identity: BBa K525515
display id: BBa_K525515
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Fusion protein of BisdA and BisdB
creator: Anna Drong
created: 2011-09-09T11:00:00Z
modified: 2015-05-08T01:12:34Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _690_
m user id: 0
owner id: 8543
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: Fusion protein of BisdA and BisdB
mutable notes: Assembled
mutable provenance: BBa_K123000 and BBa_K123001
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: K123000
sequence annotation: Freiburg scar
sequence annotation: TAA
sequence annotation: ATG
sequence annotation: AgeI
sequence annotation: K123001
sequence annotation: NgoMIV
sequence: https://synbiohub.org/public/igem/BBa_K525515_sequence/1

K123000
type: SequenceAnnotation
persistent identity: annotation2126909
display id: annotation2126909
version: 1
top level: BisdAB
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K525515/annotation2126909/range2126909/1

https://synbiohub.org/public/igem/BBa_K525515/annotation2126909/range2126909/1
type: Range
persistent identity: range2126909
display id: range2126909
version: 1
direction: direction/forward
top level: BisdAB
start: 1
end: 321
orientation: inline

Freiburg scar
type: SequenceAnnotation
persistent identity: annotation2128161
display id: annotation2128161
version: 1
top level: BisdAB
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K525515/annotation2128161/range2128161/1

https://synbiohub.org/public/igem/BBa_K525515/annotation2128161/range2128161/1
type: Range
persistent identity: range2128161
display id: range2128161
version: 1
direction: direction/forward
top level: BisdAB
start: 322
end: 327
orientation: inline

TAA
type: SequenceAnnotation
persistent identity: annotation2128159
display id: annotation2128159
version: 1
top level: BisdAB
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K525515/annotation2128159/range2128159/1

https://synbiohub.org/public/igem/BBa_K525515/annotation2128159/range2128159/1
type: Range
persistent identity: range2128159
display id: range2128159
version: 1
direction: direction/forward
top level: BisdAB
start: 1599
end: 1602
orientation: inline

ATG
type: SequenceAnnotation
persistent identity: annotation2128158
display id: annotation2128158
version: 1
top level: BisdAB
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K525515/annotation2128158/range2128158/1

https://synbiohub.org/public/igem/BBa_K525515/annotation2128158/range2128158/1
type: Range
persistent identity: range2128158
display id: range2128158
version: 1
direction: direction/forward
top level: BisdAB
start: 1
end: 3
orientation: inline

AgeI
type: SequenceAnnotation
persistent identity: annotation2128157
display id: annotation2128157
version: 1
top level: BisdAB
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K525515/annotation2128157/range2128157/1

https://synbiohub.org/public/igem/BBa_K525515/annotation2128157/range2128157/1
type: Range
persistent identity: range2128157
display id: range2128157
version: 1
direction: direction/forward
top level: BisdAB
start: 1594
end: 1599
orientation: inline

K123001
type: SequenceAnnotation
persistent identity: annotation2126910
display id: annotation2126910
version: 1
top level: BisdAB
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K525515/annotation2126910/range2126910/1

https://synbiohub.org/public/igem/BBa_K525515/annotation2126910/range2126910/1
type: Range
persistent identity: range2126910
display id: range2126910
version: 1
direction: direction/forward
top level: BisdAB
start: 328
end: 1602
orientation: inline

NgoMIV
type: SequenceAnnotation
persistent identity: annotation2128156
display id: annotation2128156
version: 1
top level: BisdAB
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K525515/annotation2128156/range2128156/1

https://synbiohub.org/public/igem/BBa_K525515/annotation2128156/range2128156/1
type: Range
persistent identity: range2128156
display id: range2128156
version: 1
direction: direction/forward
top level: BisdAB
start: 4
end: 9
orientation: inline

SbpA | CFP
type: ComponentDefinition
persistent identity: BBa K525406
display id: BBa_K525406
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Fusion Protein of S-Layer SbpA and mCerulean
creator: Timo Wolf
created: 2011-09-09T11:00:00Z
modified: 2016-01-26T09:45:16Z
discontinued: false
dominant: false
experience: experience/None
group u list: _690_
m user id: 4206
owner id: 6668
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: Fusion Protein of S-Layer SgsE and mCerulean
mutable notes: Fusion Protein of BBa_K525403 and BBa_J18930
mutable provenance: Fusion Protein of S-Layer SgsE and mCerulean
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: J18930
sequence annotation: lac operator
sequence annotation: K525401
sequence annotation: ATG
sequence annotation: Freiburg scar
sequence annotation: SbpA domain
sequence annotation: TAA
sequence annotation: NgoMIV
sequence annotation: B0034
sequence annotation: mCerulean (CFP)
sequence annotation: AgeI
sequence annotation: T7 promotoer
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_K525406_sequence/1

J18930
type: SequenceAnnotation
persistent identity: annotation2129925
display id: annotation2129925
version: 1
top level: SbpA | CFP
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K525406/annotation2129925/range2129925/1

https://synbiohub.org/public/igem/BBa_K525406/annotation2129925/range2129925/1
type: Range
persistent identity: range2129925
display id: range2129925
version: 1
direction: direction/forward
top level: SbpA | CFP
start: 3199
end: 3921
orientation: inline

lac operator
type: SequenceAnnotation
persistent identity: annotation2129917
display id: annotation2129917
version: 1
top level: SbpA | CFP
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K525406/annotation2129917/range2129917/1

https://synbiohub.org/public/igem/BBa_K525406/annotation2129917/range2129917/1
type: Range
persistent identity: range2129917
display id: range2129917
version: 1
direction: direction/forward
top level: SbpA | CFP
start: 20
end: 44
orientation: inline

K525401
type: SequenceAnnotation
persistent identity: annotation2129920
display id: annotation2129920
version: 1
top level: SbpA | CFP
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K525406/annotation2129920/range2129920/1

https://synbiohub.org/public/igem/BBa_K525406/annotation2129920/range2129920/1
type: Range
persistent identity: range2129920
display id: range2129920
version: 1
direction: direction/forward
top level: SbpA | CFP
start: 73
end: 3192
orientation: inline

ATG
type: SequenceAnnotation
persistent identity: annotation2129922
display id: annotation2129922
version: 1
top level: SbpA | CFP
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K525406/annotation2129922/range2129922/1

https://synbiohub.org/public/igem/BBa_K525406/annotation2129922/range2129922/1
type: Range
persistent identity: range2129922
display id: range2129922
version: 1
direction: direction/forward
top level: SbpA | CFP
start: 73
end: 75
orientation: inline

Freiburg scar
type: SequenceAnnotation
persistent identity: annotation2129924
display id: annotation2129924
version: 1
top level: SbpA | CFP
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K525406/annotation2129924/range2129924/1

https://synbiohub.org/public/igem/BBa_K525406/annotation2129924/range2129924/1
type: Range
persistent identity: range2129924
display id: range2129924
version: 1
direction: direction/forward
top level: SbpA | CFP
start: 3193
end: 3198
orientation: inline

SbpA domain
type: SequenceAnnotation
persistent identity: annotation2129921
display id: annotation2129921
version: 1
top level: SbpA | CFP
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K525406/annotation2129921/range2129921/1

https://synbiohub.org/public/igem/BBa_K525406/annotation2129921/range2129921/1
type: Range
persistent identity: range2129921
display id: range2129921
version: 1
direction: direction/forward
top level: SbpA | CFP
start: 73
end: 3192
orientation: inline

TAA
type: SequenceAnnotation
persistent identity: annotation2129926
display id: annotation2129926
version: 1
top level: SbpA | CFP
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K525406/annotation2129926/range2129926/1

https://synbiohub.org/public/igem/BBa_K525406/annotation2129926/range2129926/1
type: Range
persistent identity: range2129926
display id: range2129926
version: 1
direction: direction/forward
top level: SbpA | CFP
start: 3919
end: 3921
orientation: inline

NgoMIV
type: SequenceAnnotation
persistent identity: annotation2129923
display id: annotation2129923
version: 1
top level: SbpA | CFP
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K525406/annotation2129923/range2129923/1

https://synbiohub.org/public/igem/BBa_K525406/annotation2129923/range2129923/1
type: Range
persistent identity: range2129923
display id: range2129923
version: 1
direction: direction/forward
top level: SbpA | CFP
start: 76
end: 81
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation2129918
display id: annotation2129918
version: 1
top level: SbpA | CFP
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K525406/annotation2129918/range2129918/1

https://synbiohub.org/public/igem/BBa_K525406/annotation2129918/range2129918/1
type: Range
persistent identity: range2129918
display id: range2129918
version: 1
direction: direction/forward
top level: SbpA | CFP
start: 53
end: 64
orientation: inline

mCerulean (CFP)
type: SequenceAnnotation
persistent identity: annotation2129927
display id: annotation2129927
version: 1
top level: SbpA | CFP
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K525406/annotation2129927/range2129927/1

https://synbiohub.org/public/igem/BBa_K525406/annotation2129927/range2129927/1
type: Range
persistent identity: range2129927
display id: range2129927
version: 1
direction: direction/forward
top level: SbpA | CFP
start: 3199
end: 3921
orientation: inline

AgeI
type: SequenceAnnotation
persistent identity: annotation2129928
display id: annotation2129928
version: 1
top level: SbpA | CFP
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K525406/annotation2129928/range2129928/1

https://synbiohub.org/public/igem/BBa_K525406/annotation2129928/range2129928/1
type: Range
persistent identity: range2129928
display id: range2129928
version: 1
direction: direction/forward
top level: SbpA | CFP
start: 3913
end: 3918
orientation: inline

T7 promotoer
type: SequenceAnnotation
persistent identity: annotation2129916
display id: annotation2129916
version: 1
top level: SbpA | CFP
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K525406/annotation2129916/range2129916/1

https://synbiohub.org/public/igem/BBa_K525406/annotation2129916/range2129916/1
type: Range
persistent identity: range2129916
display id: range2129916
version: 1
direction: direction/forward
top level: SbpA | CFP
start: 1
end: 20
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2129919
display id: annotation2129919
version: 1
top level: SbpA | CFP
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K525406/annotation2129919/range2129919/1

https://synbiohub.org/public/igem/BBa_K525406/annotation2129919/range2129919/1
type: Range
persistent identity: range2129919
display id: range2129919
version: 1
direction: direction/forward
top level: SbpA | CFP
start: 56
end: 59
orientation: inline

Tm-Fluolin
type: ComponentDefinition
persistent identity: BBa K157016
display id: BBa_K157016
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Tm-Fluolinker-cCFP
creator: iGEM Team Freiburg 2008
created: 2008-10-25T11:00:00Z
modified: 2015-05-08T01:10:54Z
discontinued: false
dominant: false
experience: experience/None
group u list: _232_
m user id: 0
owner id: 1675
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: C-terminal part of the Split-fluorophore CFP, fused to the trans-membrane region of the Erbb1/EGF-receptor with the fluo-linker in between.
mutable notes: none
mutable provenance: Derived by cloning of the parts BBa_K157002, BBa_K157009 and BBa_K157005. For further information see the description of the parts mentioned above.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: Scar (Thr, Gly)
sequence annotation: Fluolinker
sequence annotation: C-Term_CFP
sequence annotation: AgeI
sequence annotation: NgoMI
sequence annotation: Tm-Fluolinker-cCFP
sequence annotation: Tm
sequence annotation: Sca (Thr, Gly)
sequence: https://synbiohub.org/public/igem/BBa_K157016_sequence/1

Scar (Thr, Gly)
type: SequenceAnnotation
persistent identity: annotation1991281
display id: annotation1991281
version: 1
top level: Tm-Fluolin
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157016/annotation1991281/range1991281/1

https://synbiohub.org/public/igem/BBa_K157016/annotation1991281/range1991281/1
type: Range
persistent identity: range1991281
display id: range1991281
version: 1
direction: direction/forward
top level: Tm-Fluolin
start: 79
end: 84
orientation: inline

Fluolinker
type: SequenceAnnotation
persistent identity: annotation1991282
display id: annotation1991282
version: 1
top level: Tm-Fluolin
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157016/annotation1991282/range1991282/1

https://synbiohub.org/public/igem/BBa_K157016/annotation1991282/range1991282/1
type: Range
persistent identity: range1991282
display id: range1991282
version: 1
direction: direction/forward
top level: Tm-Fluolin
start: 85
end: 135
orientation: inline

C-Term_CFP
type: SequenceAnnotation
persistent identity: annotation1991284
display id: annotation1991284
version: 1
top level: Tm-Fluolin
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157016/annotation1991284/range1991284/1

https://synbiohub.org/public/igem/BBa_K157016/annotation1991284/range1991284/1
type: Range
persistent identity: range1991284
display id: range1991284
version: 1
direction: direction/forward
top level: Tm-Fluolin
start: 142
end: 384
orientation: inline

AgeI
type: SequenceAnnotation
persistent identity: annotation1989970
display id: annotation1989970
version: 1
top level: Tm-Fluolin
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157016/annotation1989970/range1989970/1

https://synbiohub.org/public/igem/BBa_K157016/annotation1989970/range1989970/1
type: Range
persistent identity: range1989970
display id: range1989970
version: 1
direction: direction/forward
top level: Tm-Fluolin
start: 385
end: 390
orientation: inline

NgoMI
type: SequenceAnnotation
persistent identity: annotation1989969
display id: annotation1989969
version: 1
top level: Tm-Fluolin
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157016/annotation1989969/range1989969/1

https://synbiohub.org/public/igem/BBa_K157016/annotation1989969/range1989969/1
type: Range
persistent identity: range1989969
display id: range1989969
version: 1
direction: direction/forward
top level: Tm-Fluolin
start: 4
end: 9
orientation: inline

Tm-Fluolinker-cCFP
type: SequenceAnnotation
persistent identity: annotation1989971
display id: annotation1989971
version: 1
top level: Tm-Fluolin
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K157016/annotation1989971/range1989971/1

https://synbiohub.org/public/igem/BBa_K157016/annotation1989971/range1989971/1
type: Range
persistent identity: range1989971
display id: range1989971
version: 1
direction: direction/forward
top level: Tm-Fluolin
start: 1
end: 390
orientation: inline

Tm
type: SequenceAnnotation
persistent identity: annotation1991280
display id: annotation1991280
version: 1
top level: Tm-Fluolin
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157016/annotation1991280/range1991280/1

https://synbiohub.org/public/igem/BBa_K157016/annotation1991280/range1991280/1
type: Range
persistent identity: range1991280
display id: range1991280
version: 1
direction: direction/forward
top level: Tm-Fluolin
start: 10
end: 78
orientation: inline

Sca (Thr, Gly)
type: SequenceAnnotation
persistent identity: annotation1991283
display id: annotation1991283
version: 1
top level: Tm-Fluolin
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157016/annotation1991283/range1991283/1

https://synbiohub.org/public/igem/BBa_K157016/annotation1991283/range1991283/1
type: Range
persistent identity: range1991283
display id: range1991283
version: 1
direction: direction/forward
top level: Tm-Fluolin
start: 136
end: 141
orientation: inline

SbpA
type: ComponentDefinition
persistent identity: BBa K525401
display id: BBa_K525401
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: S-Layer SbpA from Lysinibacillus sphaericus
creator: Timo Wolf
created: 2011-09-09T11:00:00Z
modified: 2015-05-08T01:12:34Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _690_
m user id: 0
owner id: 6668
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: S-Layer sbpA from Lysinibacillus sphaericus
mutable notes: Synthesis
mutable provenance: Synthesis
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: SbpA domain
sequence annotation: AgeI
sequence annotation: TAA
sequence annotation: ATG
sequence annotation: NgoMIV
sequence annotation: SbpA
sequence: https://synbiohub.org/public/igem/BBa_K525401_sequence/1

SbpA domain
type: SequenceAnnotation
persistent identity: annotation2129989
display id: annotation2129989
version: 1
top level: SbpA
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K525401/annotation2129989/range2129989/1

https://synbiohub.org/public/igem/BBa_K525401/annotation2129989/range2129989/1
type: Range
persistent identity: range2129989
display id: range2129989
version: 1
direction: direction/forward
top level: SbpA
start: 1
end: 3129
orientation: inline

AgeI
type: SequenceAnnotation
persistent identity: annotation2129993
display id: annotation2129993
version: 1
top level: SbpA
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K525401/annotation2129993/range2129993/1

https://synbiohub.org/public/igem/BBa_K525401/annotation2129993/range2129993/1
type: Range
persistent identity: range2129993
display id: range2129993
version: 1
direction: direction/forward
top level: SbpA
start: 3121
end: 3126
orientation: inline

TAA
type: SequenceAnnotation
persistent identity: annotation2129992
display id: annotation2129992
version: 1
top level: SbpA
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K525401/annotation2129992/range2129992/1

https://synbiohub.org/public/igem/BBa_K525401/annotation2129992/range2129992/1
type: Range
persistent identity: range2129992
display id: range2129992
version: 1
direction: direction/forward
top level: SbpA
start: 3127
end: 3129
orientation: inline

ATG
type: SequenceAnnotation
persistent identity: annotation2129990
display id: annotation2129990
version: 1
top level: SbpA
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K525401/annotation2129990/range2129990/1

https://synbiohub.org/public/igem/BBa_K525401/annotation2129990/range2129990/1
type: Range
persistent identity: range2129990
display id: range2129990
version: 1
direction: direction/forward
top level: SbpA
start: 1
end: 3
orientation: inline

NgoMIV
type: SequenceAnnotation
persistent identity: annotation2129991
display id: annotation2129991
version: 1
top level: SbpA
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K525401/annotation2129991/range2129991/1

https://synbiohub.org/public/igem/BBa_K525401/annotation2129991/range2129991/1
type: Range
persistent identity: range2129991
display id: range2129991
version: 1
direction: direction/forward
top level: SbpA
start: 4
end: 9
orientation: inline

SbpA
type: SequenceAnnotation
persistent identity: annotation2129988
display id: annotation2129988
version: 1
top level: SbpA
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K525401/annotation2129988/range2129988/1

https://synbiohub.org/public/igem/BBa_K525401/annotation2129988/range2129988/1
type: Range
persistent identity: range2129988
display id: range2129988
version: 1
direction: direction/forward
top level: SbpA
start: 1
end: 3129
orientation: inline

CspB | RFP
type: ComponentDefinition
persistent identity: BBa K525234
display id: BBa_K525234
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Fusion Protein of mRFP, S-layer cspB from Corynebacterium halotolerans with TAT-sequence, PT7, RBS
creator: Timo Wolf
created: 2011-09-19T11:00:00Z
modified: 2016-01-25T01:34:53Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _690_
m user id: 4206
owner id: 6668
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Fusion Protein of mRFP and S-layer cspB from Corynebacterium halotolerans with TAT-sequence, PT7 and RBS
mutable notes: Gibson Assembly of BBa_E1010 and BBa_K525224
mutable provenance: Gibson Assembly of BBa_E1010 and BBa_K525224
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: BBa_E1010
sequence annotation: mRFP
sequence annotation: cspB domain
sequence annotation: TAT-sequence
sequence annotation: T7 promoter
sequence annotation: BBa_B0034
sequence annotation: RBS
sequence annotation: Linker
sequence annotation: K525222
sequence annotation: TAA
sequence: https://synbiohub.org/public/igem/BBa_K525234_sequence/1

BBa_E1010
type: SequenceAnnotation
persistent identity: annotation2161288
display id: annotation2161288
version: 1
top level: CspB | RFP
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K525234/annotation2161288/range2161288/1

https://synbiohub.org/public/igem/BBa_K525234/annotation2161288/range2161288/1
type: Range
persistent identity: range2161288
display id: range2161288
version: 1
direction: direction/forward
top level: CspB | RFP
start: 129
end: 799
orientation: inline

mRFP
type: SequenceAnnotation
persistent identity: annotation2161287
display id: annotation2161287
version: 1
top level: CspB | RFP
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K525234/annotation2161287/range2161287/1

https://synbiohub.org/public/igem/BBa_K525234/annotation2161287/range2161287/1
type: Range
persistent identity: range2161287
display id: range2161287
version: 1
direction: direction/forward
top level: CspB | RFP
start: 129
end: 799
orientation: inline

cspB domain
type: SequenceAnnotation
persistent identity: annotation2161290
display id: annotation2161290
version: 1
top level: CspB | RFP
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K525234/annotation2161290/range2161290/1

https://synbiohub.org/public/igem/BBa_K525234/annotation2161290/range2161290/1
type: Range
persistent identity: range2161290
display id: range2161290
version: 1
direction: direction/forward
top level: CspB | RFP
start: 809
end: 2189
orientation: inline

TAT-sequence
type: SequenceAnnotation
persistent identity: annotation2161286
display id: annotation2161286
version: 1
top level: CspB | RFP
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K525234/annotation2161286/range2161286/1

https://synbiohub.org/public/igem/BBa_K525234/annotation2161286/range2161286/1
type: Range
persistent identity: range2161286
display id: range2161286
version: 1
direction: direction/forward
top level: CspB | RFP
start: 34
end: 128
orientation: inline

T7 promoter
type: SequenceAnnotation
persistent identity: annotation2161283
display id: annotation2161283
version: 1
top level: CspB | RFP
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K525234/annotation2161283/range2161283/1

https://synbiohub.org/public/igem/BBa_K525234/annotation2161283/range2161283/1
type: Range
persistent identity: range2161283
display id: range2161283
version: 1
direction: direction/forward
top level: CspB | RFP
start: 1
end: 20
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2161284
display id: annotation2161284
version: 1
top level: CspB | RFP
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K525234/annotation2161284/range2161284/1

https://synbiohub.org/public/igem/BBa_K525234/annotation2161284/range2161284/1
type: Range
persistent identity: range2161284
display id: range2161284
version: 1
direction: direction/forward
top level: CspB | RFP
start: 21
end: 33
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2161285
display id: annotation2161285
version: 1
top level: CspB | RFP
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K525234/annotation2161285/range2161285/1

https://synbiohub.org/public/igem/BBa_K525234/annotation2161285/range2161285/1
type: Range
persistent identity: range2161285
display id: range2161285
version: 1
direction: direction/forward
top level: CspB | RFP
start: 25
end: 28
orientation: inline

Linker
type: SequenceAnnotation
persistent identity: annotation2161289
display id: annotation2161289
version: 1
top level: CspB | RFP
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K525234/annotation2161289/range2161289/1

https://synbiohub.org/public/igem/BBa_K525234/annotation2161289/range2161289/1
type: Range
persistent identity: range2161289
display id: range2161289
version: 1
direction: direction/forward
top level: CspB | RFP
start: 800
end: 808
orientation: inline

K525222
type: SequenceAnnotation
persistent identity: annotation2161282
display id: annotation2161282
version: 1
top level: CspB | RFP
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K525234/annotation2161282/range2161282/1

https://synbiohub.org/public/igem/BBa_K525234/annotation2161282/range2161282/1
type: Range
persistent identity: range2161282
display id: range2161282
version: 1
direction: direction/forward
top level: CspB | RFP
start: 809
end: 2189
orientation: inline

TAA
type: SequenceAnnotation
persistent identity: annotation2161291
display id: annotation2161291
version: 1
top level: CspB | RFP
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K525234/annotation2161291/range2161291/1

https://synbiohub.org/public/igem/BBa_K525234/annotation2161291/range2161291/1
type: Range
persistent identity: range2161291
display id: range2161291
version: 1
direction: direction/forward
top level: CspB | RFP
start: 2187
end: 2189
orientation: inline

Tm-nCFP
type: ComponentDefinition
persistent identity: BBa K157017
display id: BBa_K157017
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Tm-nCFP
creator: Freiburg iGEM Team 08
created: 2008-10-25T11:00:00Z
modified: 2015-05-08T01:10:54Z
discontinued: false
dominant: false
experience: experience/None
group u list: _232_
m user id: 0
owner id: 1673
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: N-terminal part of the Split-fluorophore CFP, fused to the trans-membrane region of the Erbb1/EGF-receptor.
mutable notes: none
mutable provenance: Derived by cloning parts BBa_K157002, BBa_K157006. For further information see the description of the parts mentioned above.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: Tm-nCFP
sequence annotation: scar (Thr, Gly)
sequence annotation: N-Term_CFP
sequence annotation: NgoMI
sequence annotation: AgeI
sequence annotation: Tm
sequence: https://synbiohub.org/public/igem/BBa_K157017_sequence/1

Tm-nCFP
type: SequenceAnnotation
persistent identity: annotation1989979
display id: annotation1989979
version: 1
top level: Tm-nCFP
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K157017/annotation1989979/range1989979/1

https://synbiohub.org/public/igem/BBa_K157017/annotation1989979/range1989979/1
type: Range
persistent identity: range1989979
display id: range1989979
version: 1
direction: direction/forward
top level: Tm-nCFP
start: 1
end: 555
orientation: inline

scar (Thr, Gly)
type: SequenceAnnotation
persistent identity: annotation1991286
display id: annotation1991286
version: 1
top level: Tm-nCFP
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157017/annotation1991286/range1991286/1

https://synbiohub.org/public/igem/BBa_K157017/annotation1991286/range1991286/1
type: Range
persistent identity: range1991286
display id: range1991286
version: 1
direction: direction/forward
top level: Tm-nCFP
start: 79
end: 84
orientation: inline

N-Term_CFP
type: SequenceAnnotation
persistent identity: annotation1991287
display id: annotation1991287
version: 1
top level: Tm-nCFP
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157017/annotation1991287/range1991287/1

https://synbiohub.org/public/igem/BBa_K157017/annotation1991287/range1991287/1
type: Range
persistent identity: range1991287
display id: range1991287
version: 1
direction: direction/forward
top level: Tm-nCFP
start: 85
end: 549
orientation: inline

NgoMI
type: SequenceAnnotation
persistent identity: annotation1989977
display id: annotation1989977
version: 1
top level: Tm-nCFP
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157017/annotation1989977/range1989977/1

https://synbiohub.org/public/igem/BBa_K157017/annotation1989977/range1989977/1
type: Range
persistent identity: range1989977
display id: range1989977
version: 1
direction: direction/forward
top level: Tm-nCFP
start: 4
end: 9
orientation: inline

AgeI
type: SequenceAnnotation
persistent identity: annotation1989978
display id: annotation1989978
version: 1
top level: Tm-nCFP
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157017/annotation1989978/range1989978/1

https://synbiohub.org/public/igem/BBa_K157017/annotation1989978/range1989978/1
type: Range
persistent identity: range1989978
display id: range1989978
version: 1
direction: direction/forward
top level: Tm-nCFP
start: 550
end: 555
orientation: inline

Tm
type: SequenceAnnotation
persistent identity: annotation1991285
display id: annotation1991285
version: 1
top level: Tm-nCFP
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157017/annotation1991285/range1991285/1

https://synbiohub.org/public/igem/BBa_K157017/annotation1991285/range1991285/1
type: Range
persistent identity: range1991285
display id: range1991285
version: 1
direction: direction/forward
top level: Tm-nCFP
start: 10
end: 78
orientation: inline

SP-AntiNIP
type: ComponentDefinition
persistent identity: BBa K157022
display id: BBa_K157022
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: SP-AntiNIP-GGGSlinker
creator: iGEM Team Freiburg 2008
created: 2008-10-25T11:00:00Z
modified: 2015-05-08T01:10:54Z
discontinued: false
dominant: false
experience: experience/None
group u list: _232_
m user id: 0
owner id: 1673
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: The scFv-anti-NIP (with a flexible GGGS-linker at the C-terminus) fused to the signal peptide of Erbb1/EGF-receptor.
mutable notes: none
mutable provenance: Derived by cloning of the parts BBa_K157001, BBa_K157003 and BBa_K157013. For further information see the description of the parts mentioned above.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: GGGS-Linker
sequence annotation: Sc-Fv_anti-NIP
sequence annotation: NgoMI
sequence annotation: SP-AntiNIP-GGGSlinker
sequence annotation: Scar (Thr, Gly)
sequence annotation: AgeI
sequence annotation: SP
sequence: https://synbiohub.org/public/igem/BBa_K157022_sequence/1

GGGS-Linker
type: SequenceAnnotation
persistent identity: annotation1991699
display id: annotation1991699
version: 1
top level: SP-AntiNIP
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157022/annotation1991699/range1991699/1

https://synbiohub.org/public/igem/BBa_K157022/annotation1991699/range1991699/1
type: Range
persistent identity: range1991699
display id: range1991699
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 828
end: 873
orientation: inline

Sc-Fv_anti-NIP
type: SequenceAnnotation
persistent identity: annotation1991631
display id: annotation1991631
version: 1
top level: SP-AntiNIP
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157022/annotation1991631/range1991631/1

https://synbiohub.org/public/igem/BBa_K157022/annotation1991631/range1991631/1
type: Range
persistent identity: range1991631
display id: range1991631
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 88
end: 822
orientation: inline

NgoMI
type: SequenceAnnotation
persistent identity: annotation1990125
display id: annotation1990125
version: 1
top level: SP-AntiNIP
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157022/annotation1990125/range1990125/1

https://synbiohub.org/public/igem/BBa_K157022/annotation1990125/range1990125/1
type: Range
persistent identity: range1990125
display id: range1990125
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 4
end: 9
orientation: inline

SP-AntiNIP-GGGSlinker
type: SequenceAnnotation
persistent identity: annotation1990157
display id: annotation1990157
version: 1
top level: SP-AntiNIP
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K157022/annotation1990157/range1990157/1

https://synbiohub.org/public/igem/BBa_K157022/annotation1990157/range1990157/1
type: Range
persistent identity: range1990157
display id: range1990157
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 1
end: 879
orientation: inline

Scar (Thr, Gly)
type: SequenceAnnotation
persistent identity: annotation1991580
display id: annotation1991580
version: 1
top level: SP-AntiNIP
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157022/annotation1991580/range1991580/1

https://synbiohub.org/public/igem/BBa_K157022/annotation1991580/range1991580/1
type: Range
persistent identity: range1991580
display id: range1991580
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 82
end: 87
orientation: inline

Scar (Thr, Gly)
type: SequenceAnnotation
persistent identity: annotation1991698
display id: annotation1991698
version: 1
top level: SP-AntiNIP
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157022/annotation1991698/range1991698/1

https://synbiohub.org/public/igem/BBa_K157022/annotation1991698/range1991698/1
type: Range
persistent identity: range1991698
display id: range1991698
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 823
end: 828
orientation: inline

AgeI
type: SequenceAnnotation
persistent identity: annotation1990141
display id: annotation1990141
version: 1
top level: SP-AntiNIP
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157022/annotation1990141/range1990141/1

https://synbiohub.org/public/igem/BBa_K157022/annotation1990141/range1990141/1
type: Range
persistent identity: range1990141
display id: range1990141
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 874
end: 879
orientation: inline

SP
type: SequenceAnnotation
persistent identity: annotation1991579
display id: annotation1991579
version: 1
top level: SP-AntiNIP
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157022/annotation1991579/range1991579/1

https://synbiohub.org/public/igem/BBa_K157022/annotation1991579/range1991579/1
type: Range
persistent identity: range1991579
display id: range1991579
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 10
end: 81
orientation: inline

SgsE | RFP
type: ComponentDefinition
persistent identity: BBa K525304
display id: BBa_K525304
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Fusion Protein of S-Layer SgsE and mCherry RFP
creator: Timo Wolf
created: 2011-09-09T11:00:00Z
modified: 2016-01-26T10:06:30Z
discontinued: false
dominant: false
experience: experience/None
group u list: _690_
m user id: 4206
owner id: 6668
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Fusion Protein of S-Layer SgsE and mCherry RFP
mutable notes: Fusion Protein of BBa_K525303 and BBa_J18932
mutable provenance: Fusion Protein of S-Layer SgsE and mCherry RFP
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: TAA
sequence annotation: mCherry (RFP)
sequence annotation: Freiburg Scar
sequence annotation: lac operator
sequence annotation: B0034
sequence annotation: J18932
sequence annotation: K525301
sequence annotation: T7 promoter
sequence annotation: ATG
sequence annotation: Age I
sequence annotation: SgsE domain
sequence annotation: NgoMIV
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_K525304_sequence/1

TAA
type: SequenceAnnotation
persistent identity: annotation2128567
display id: annotation2128567
version: 1
top level: SgsE | RFP
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K525304/annotation2128567/range2128567/1

https://synbiohub.org/public/igem/BBa_K525304/annotation2128567/range2128567/1
type: Range
persistent identity: range2128567
display id: range2128567
version: 1
direction: direction/forward
top level: SgsE | RFP
start: 3118
end: 3120
orientation: inline

mCherry (RFP)
type: SequenceAnnotation
persistent identity: annotation2128565
display id: annotation2128565
version: 1
top level: SgsE | RFP
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K525304/annotation2128565/range2128565/1

https://synbiohub.org/public/igem/BBa_K525304/annotation2128565/range2128565/1
type: Range
persistent identity: range2128565
display id: range2128565
version: 1
direction: direction/forward
top level: SgsE | RFP
start: 2407
end: 3120
orientation: inline

Freiburg Scar
type: SequenceAnnotation
persistent identity: annotation2128586
display id: annotation2128586
version: 1
top level: SgsE | RFP
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K525304/annotation2128586/range2128586/1

https://synbiohub.org/public/igem/BBa_K525304/annotation2128586/range2128586/1
type: Range
persistent identity: range2128586
display id: range2128586
version: 1
direction: direction/forward
top level: SgsE | RFP
start: 2401
end: 2406
orientation: inline

lac operator
type: SequenceAnnotation
persistent identity: annotation2128568
display id: annotation2128568
version: 1
top level: SgsE | RFP
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K525304/annotation2128568/range2128568/1

https://synbiohub.org/public/igem/BBa_K525304/annotation2128568/range2128568/1
type: Range
persistent identity: range2128568
display id: range2128568
version: 1
direction: direction/forward
top level: SgsE | RFP
start: 20
end: 44
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation2128562
display id: annotation2128562
version: 1
top level: SgsE | RFP
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K525304/annotation2128562/range2128562/1

https://synbiohub.org/public/igem/BBa_K525304/annotation2128562/range2128562/1
type: Range
persistent identity: range2128562
display id: range2128562
version: 1
direction: direction/forward
top level: SgsE | RFP
start: 53
end: 64
orientation: inline

J18932
type: SequenceAnnotation
persistent identity: annotation2128561
display id: annotation2128561
version: 1
top level: SgsE | RFP
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K525304/annotation2128561/range2128561/1

https://synbiohub.org/public/igem/BBa_K525304/annotation2128561/range2128561/1
type: Range
persistent identity: range2128561
display id: range2128561
version: 1
direction: direction/forward
top level: SgsE | RFP
start: 2407
end: 3120
orientation: inline

K525301
type: SequenceAnnotation
persistent identity: annotation2128564
display id: annotation2128564
version: 1
top level: SgsE | RFP
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K525304/annotation2128564/range2128564/1

https://synbiohub.org/public/igem/BBa_K525304/annotation2128564/range2128564/1
type: Range
persistent identity: range2128564
display id: range2128564
version: 1
direction: direction/forward
top level: SgsE | RFP
start: 71
end: 2400
orientation: inline

T7 promoter
type: SequenceAnnotation
persistent identity: annotation2128563
display id: annotation2128563
version: 1
top level: SgsE | RFP
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K525304/annotation2128563/range2128563/1

https://synbiohub.org/public/igem/BBa_K525304/annotation2128563/range2128563/1
type: Range
persistent identity: range2128563
display id: range2128563
version: 1
direction: direction/forward
top level: SgsE | RFP
start: 1
end: 20
orientation: inline

ATG
type: SequenceAnnotation
persistent identity: annotation2128570
display id: annotation2128570
version: 1
top level: SgsE | RFP
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K525304/annotation2128570/range2128570/1

https://synbiohub.org/public/igem/BBa_K525304/annotation2128570/range2128570/1
type: Range
persistent identity: range2128570
display id: range2128570
version: 1
direction: direction/forward
top level: SgsE | RFP
start: 71
end: 73
orientation: inline

Age I
type: SequenceAnnotation
persistent identity: annotation2128571
display id: annotation2128571
version: 1
top level: SgsE | RFP
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K525304/annotation2128571/range2128571/1

https://synbiohub.org/public/igem/BBa_K525304/annotation2128571/range2128571/1
type: Range
persistent identity: range2128571
display id: range2128571
version: 1
direction: direction/forward
top level: SgsE | RFP
start: 3112
end: 3117
orientation: inline

SgsE domain
type: SequenceAnnotation
persistent identity: annotation2128566
display id: annotation2128566
version: 1
top level: SgsE | RFP
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K525304/annotation2128566/range2128566/1

https://synbiohub.org/public/igem/BBa_K525304/annotation2128566/range2128566/1
type: Range
persistent identity: range2128566
display id: range2128566
version: 1
direction: direction/forward
top level: SgsE | RFP
start: 71
end: 2400
orientation: inline

NgoMIV
type: SequenceAnnotation
persistent identity: annotation2128572
display id: annotation2128572
version: 1
top level: SgsE | RFP
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K525304/annotation2128572/range2128572/1

https://synbiohub.org/public/igem/BBa_K525304/annotation2128572/range2128572/1
type: Range
persistent identity: range2128572
display id: range2128572
version: 1
direction: direction/forward
top level: SgsE | RFP
start: 76
end: 81
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2128569
display id: annotation2128569
version: 1
top level: SgsE | RFP
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K525304/annotation2128569/range2128569/1

https://synbiohub.org/public/igem/BBa_K525304/annotation2128569/range2128569/1
type: Range
persistent identity: range2128569
display id: range2128569
version: 1
direction: direction/forward
top level: SgsE | RFP
start: 56
end: 59
orientation: inline