N-terminal part of the Split-flourophore YFP, fused to the trans-membrane region of the Erbb1/EGF-receptor.
Derived by cloning the parts BBa_K157002, BBa_K157008. For further information see the description of the parts mentioned above.
Fusion Protein of S-Layer SbpA and mCitrine
Fusion Protein of BBa_K525403 and BBa_J18931
Fusion protein of NADP+ Oxidoreductase and BisdA and BisdB
Fusion protein of NADP+ Oxidoreduktase and BisdA and BisdB; RFC 25
N-terminal fragment (Amino acids1-156) of the monomeric[5] yellow fluorescent protein variant ???Venus??? [4], designed for fusion/BiFC[1,3]. Reassembly with Part Bba_K157007 (Split-Venus-cYFP) generates the complete, working YFP ???Venus???, whereas combination with the C-terminal fragment (Part Bba_K157005) of the monomeric cyan fluorescent Protein ???CeruleanR206K??? (Part Bba_I757009) results in green fluorescence[2].See "Part Design" for references.
Between BioBrick 1.0 sites this part is flanked 5' by NgoMI(=NgoMIV) and 3' AgeI(=PinAI) to facilitate in frame cloning for protein fusions.
Gene synthesis by GeneArt, optimized for expression in homo sapiens.
A composite part consisting of the signal peptide of Erbb1/EGF-receptor, scFV-antiNIP, GGGSflexlinker (extracellular part), the trans-membrane region of the Erbb1/EGF-receptor and the C-terminal part of the split fluorophor YFP with a fluolinker joined to its N-Terminus (intracellular part).
Derived by cloning of the parts BBa_K157001, BBa_K157004, BBa_K157013, BBa_K157002 and first half of split Luciferase For further information see the description of the parts mentioned above.
NADP+ Oxidoreductase from Escherichia coli
Fusion protein of SgsE, NADP+ Oxidoreductase, BisdA and BisdB with PT7/lac
PT7 + RBS + SgsE || Red || BisdA || BisdB ; RCU 25
Part consists of the signal peptide of Erbb1/EGF-receptor, scFV-antiNIP, GGGSflexlinker (extracellular part), the trans-membrane region of the Erbb1/EGF-receptor and the C-terminal part of the split fluorophor YFP with a fluolinker joined to its N-Terminus (intracellular part).
Derived by cloning of the parts BBa_K157001, BBa_K157003, BBa_K157013, BBa_K157002, BBa_K157006. For further information see the description of the parts mentioned above.
Part 1 of the Split- Luciferase fused to the trans-membrane region of the Erbb1/EGF-receptor.
Derived by cloning of the parts BBa_K157002 and Split-Luciferase fragment 1. For further information see the description of the parts mentioned above.
Assembled via Freiburg Standard Assembly (RFC 25)
Assembled fusion protein. SbpA was synthesized, LigA is the natural ligase A of E. coli
Derived by cloning of the parts BBa_K157001, BBa_K157003, BBa_K157013, BBa_K157002, BBa_K157009 and BBa_K157005. For further information see the description of the parts mentioned above.
Derived by cloning of the parts BBa_K157001, BBa_K157003, BBa_K157013, BBa_K157002, BBa_K157009 and BBa_K157007. For further information see the description of the parts mentioned above.
Transmembrane region of the EGF-Receptor (ErbB-1)
Helical, single-span transmembrane region of the human EGF-Receptor type ErbB-1, sequence taken from UniProtKB/Swiss-Prot entry P00533.
Transmembrane region of the human EGF-Receptor type ErbB-1. Sequence taken from UniProtKB/Swiss-Prot entry P00533. Gene synthesis by ATG:biosynthetics, optimized for expression in homo sapiens by iGEM-Team Freiburg 2008.
Fusion Protein of S-Layer SbpA and mCherry RFP
Fusion Protein of BBa_K525403 and BBa_J18932
EnvZ Signal Transduction and Kinase Domains
E. coli EnvZ (ncbi: https://www.ncbi.nlm.nih.gov/gene/947272)
S-layer cspB from Corynebacterium halotolerans
S-layer cspB from Corynebacterium halotolerans with lipid anchor, PT7 and RBS
S-layer cspB from Corynebacterium halotolerans with TAT-Sequence and lipid anchor, PT7 and RBS
N-terminal fragment (Amino acids1-155) of the monomeric[5] cyan fluorescent protein variant ???Cerulean???, designed for fusion/BiFC[1,3]. Reassembly with Split-Cerulean-cCFP (Part Bba_K157005) generates the complete, working CFP ???Cerulean???, whereas combination with the C-terminal fragment (Part Bba_K157007) of the monomeric yellow fluorescent Protein ???Venus???[4] (Part Bba_I757008) results in green fluorescence[2]. - [1] Chang-Deng Hu, Yurii Chinenov, Tom K. Kerppola: ???Visualization of Interactions among bZIP and Rel Family Proteins in Living Cells Using Bimolecular Fluorescence Complementation???, Molecular Cell, Vol. 9, 789???798, April, 2002 - [2] Chang Deng Hu, Tom K. Kerppola: ???Simultaneous visualization of multiple protein interactions in living cells using multicolor fluorescence complementation analysis???, Nat Biotechnol. 2003 May; 21(5):539-545 (doi:10. 1038/nbt816) -[3] Tom K. Kerppola: ???Design and implementation of bimolecular fluorescence complementation (BiFC) assays for the visualization of protein interactions in living cells???, Nat Protoc. 2006;1(3):1278-1286 (doi:10.1038/nprot.2006.201) -[4]Nagai, T. et al. ???A variant of yellow fluorescent protein with fast and efficient maturation for cell-biological applications??? J. Biol. Chem. 276, 29188-29194, 2001 -[5]Roger Y. Tsien et al. ???Creating new fluorescent probes for cell biology???, Nature Biotechnology Reviews, Vol. 3, 906-918, 2002
Fusion protein of NADP+ Oxidoreductase and BisdA and BisdB, polycistronic
Fusion protein of NADP+ Oxidoreduktase and BisdA and BisdB, policistronic
S-layer cspB from Corynebacterium halotolerans with TAT-sequence, PT7 and RBS
S-layer cspB from Corynebacterium halotolerans with PT7 and RBS
Derived by cloning of the parts BBa_K157001, BBa_K157004, BBa_K157013, BBa_K157002, BBa_K157009 and BBa_K157007. For further information see the description of the parts mentioned above.
S-layer cspB from Corynebacterium glutamicum with TAT-Sequence and lipid anchor, PT7 and RBS
C-terminal fragment (Amino acids156-236) of the monomeric[5] cyan fluorescent protein variant ???Cerulean???, designed for fusion/BiFC[1,3]. Reassembly with Split-Cerulean-nCFP (Part Bba_K157006) generates the complete, working CFP ???Cerulean???, whereas combination with the N-terminal fragment (Part Bba_K157008) of the monomeric yellow fluorescent Protein ???Venus???[4] (Part Bba_I757008) results in green fluorescence[2].References see "Part Design".
S-Layer SgsE from Geobacillus stearothermophilus
Fusion protein of SbpA, NADP+ Oxidoreductase, BisdA and BisdB with PT7/lac
PT7 + RBS + SbpA || Red || BisdA || BisdB
Derived by cloning of the parts BBa_K157001, BBa_K157004, BBa_K157013, BBa_K157002, BBa_K157006. For further information see the description of the parts mentioned above.
Fusion Protein of S-Layer SbpA and LigA with His-Tag
Assembled with Freiburg Standard Assembly (RFC 25)
SbpA was synthesized, LigA is the native ligase of E. coli.
DNA ligase from Escherichia coli (LigA) with PT7 and RBS
First fragment of beta-Lactamase fused to the trans-membrane region of the Erbb1/EGF-receptor.
Derived by cloning of the parts BBa_K157002, BBa_I757011. For further information see the description of the parts mentioned above.
Lipocalin (with a flexible GGGS-linker at the C-terminus) fusioned to the signal peptide of Erbb1/EGF-receptor.
Derived by cloning of the parts BBa_K157001, BBa_K157003 and BBa_K157013. For further information see the description of the parts mentioned above.
Fusion protein of S-Layer SgsE with T7/lac and BisdA and BisdB
Fusion Protein of S-Layer SgsE and Firefly-Luciferase
Derived by cloning of the parts BBa_K157001, BBa_K157004, BBa_K157013, BBa_K157002 and the second half of split Luciferase For further information see the description of the parts mentioned above.
Fluoresceine A -binding derivative of a Lipocalin (BBP, bilin binding protein from pieris brassicae), originally designed by Arne Skerra [see Ref. 1,2].
Fusion protein of NADP+ Oxidoreductase and BisdA and BisdB with middle strong promoter,RBS
Fusion protein of ferredoxin-NADP+ oxidoreductase, BisdA and BisdB; RFC 25 (Freiburg BioBrick assembly standard) for characterization of intra- and extracellular BPA degradation.
Fusion protein of BisdA and BisdB behind constitutive promoter
A composite part consisting of the signal-peptide of Erbb1/EGF-receptor, scFV-antiNIP, GGGSflexlinker (extracellular part), the trans-membrane region of the Erbb1/EGF-receptor and the N-terminal part of split-enzyme beta-Lactamase (intracellular part).
Derived by cloning of the parts BBa_K157001, BBa_K157003, BBa_K157013, BBa_K157002 and BBa_I757011. For further information see the description of the parts mentioned above.
S-Layer SgsE from Geobacillus stearothermophilus with T7/lac
pT7 + RBS + S-Layer + TAT-Sequenz + Lipidanker
Fusion Protein of S-Layer SgsE and mCitrine
Fusion Protein of BBa_K525303 and BBa_J18931
A composite part consisting of the signal peptide of Erbb1/EGF-receptor, which ensures the transport to the cell membrane scFV-antiNIP, GGGSflexlinker (extracellular parts), the trans-membrane region of the Erbb1/EGF-receptor and the N-terminal part of the split fluorophor YFP(intracellular part).
Derived by cloning of the parts BBa_K157001, BBa_K157004, BBa_K157013, BBa_K157002, BBa_K157008. For further information see the description of the parts mentioned above.
Derived by cloning of the parts BBa_K157001, BBa_K157003, BBa_K157013, BBa_K157002 and Part 2 of Split- Luciferase For further information see the description of the parts mentioned above.
Derived by cloning of the parts BBa_K157001, BBa_K157004, BBa_K157013, BBa_K157002, BBa_K157009 and BBa_K157005. For further information see the description of the parts mentioned above.
Part consists of the signal peptide of Erbb1/EGF-receptor, scFV-antiNIP, GGGSflexlinker (extracellular part), the trans-membrane region of the Erbb1/EGF-receptor and the N-terminal part of the split enzyme beta-Lactamase (intracellular part).
Derived by cloning of the parts BBa_K157001, BBa_K157004, BBa_K157013, BBa_K157002 and BBa_I757011. For further information see the description of the parts mentioned above.
Second fragment of beta-Lactamase fused to the trans-membrane region of the Erbb1/EGF-receptor.
Derived by cloning of the parts BBa_K157002, BBa_I757012. For further information see the description of the parts mentioned above.
Fusion Protein of S-Layer SgsE and LigA with His-Tag
This part was assembled by Freiburg Standard Assembly (RFC 25)
SgsE was synthesized, LigA with His-Tag created by PCR. LigA is the natural ligase A of E. coli.
S-layer cspB from Corynebacterium glutamicum with lipid anchor and PT7 and RBS
C-terminal fragment (Amino acids157-237) of the monomeric[5] yellow fluorescent protein variant ???Venus??? [4], designed for fusion/BiFC[1,3]. Reassembly with Part Bba_K157008 (Split-Venus-nYFP) generates the complete, working YFP ???Venus???, whereas combination with the n-terminal fragment (Part Bba_K157006) of the monomeric cyan fluorescent Protein ???CeruleanR206K??? (Part Bba_I757009) results in green fluorescence[2]. - [1] Chang-Deng Hu, Yurii Chinenov, Tom K. Kerppola: ???Visualization of Interactions among bZIP and Rel Family Proteins in Living Cells Using Bimolecular Fluorescence Complementation???, Molecular Cell, Vol. 9, 789???798, April, 2002 - [2] Chang Deng Hu, Tom K. Kerppola: ???Simultaneous visualization of multiple protein interactions in living cells using multicolor fluorescence complementation analysis???, Nat Biotechnol. 2003 May; 21(5):539-545 (doi:10. 1038/nbt816) -[3] Tom K. Kerppola: ???Design and implementation of bimolecular fluorescence complementation (BiFC) assays for the visualization of protein interactions in living cells???, Nat Protoc. 2006;1(3):1278-1286 (doi:10.1038/nprot.2006.201) -[4]Nagai, T. et al. ???A variant of yellow fluorescent protein with fast and efficient maturation for cell-biological applications??? J. Biol. Chem. 276, 29188-29194, 2001 -[5]Roger Y. Tsien et al. ???Creating new fluorescent probes for cell biology???, Nature Biotechnology Reviews, Vol. 3, 906-918, 2002
Fusion Protein of S-Layer SgsE and mCerulean
Fusion Protein of BBa_K525303 and BBa_J18930
NOD1 (ncbi: https://www.ncbi.nlm.nih.gov/gene/10392)
Fusion Protein of S-Layer SbpA and NADP+ Oxidoreductase
Part consists of the signal peptide of Erbb1/EGF-receptor, scFV-antiNIP, GGGSflexlinker (extracellular part), the trans-membrane region of the Erbb1/EGF-receptor and the C-terminal part of the split enzyme beta-Lactamase (intracellular part).
Derived by cloning of the parts BBa_K157001, BBa_K157004, BBa_K157013, BBa_K157002 and BBa_I757012. For further information see the description of the parts mentioned above.
C-terminal part of the Split fluorophore YFP fusioned to the transmembrane region of the Erbb1/EGF-receptor with the fluolinker in between.
Derived by cloning of the parts BBa_K157002, BBa_K157009 and BBa_K157007. For further information see the description of the parts mentioned above.
A composite part consisting of the signal peptide of Erbb1/EGF-receptor, scFV-antiNIP, GGGSflexlinker (extracellular part), the trans-membrane region of the Erbb1/EGF-receptor and the C-terminal part of the split-enzyme beta-Lactamase (intracellular part).
Derived by cloning of the parts BBa_K157001, BBa_K157003, BBa_K157013, BBa_K157002 and BBa_I757012. For further information see the description of the parts mentioned above.
Singlechain Fv.Fragment of an Anti-Nitro-Iodo-Phenol-antibody B1-8. Binds the hapten NIP (Nitro-Iodo-Phenole) with an affinity of 2.0 uM[1,2]. Designed for fusion to proteins or peptides via in frame cloning.
RFC12 Maltose Binding Protein Internal Domain [malE]
This Maltose-binding protein is designed for either N-terminal or C-terminal fusions. MBP fused proteins can be purified in one step affinity chromatography using a cross-linked amylose matrix and then eluted with 10 mM maltose. Note that the amylose matrix can only be regenerated, in most cases, up to five times. The large size of this tag can also affect the fused protein. MBP fusions often have higher levels of expression and solubility that the native untagged protein, with typical yields of up to 40 mg/liter culture.
pMAL-c2x
Fusion Protein of S-Layer SgsE and Oxidoreductase
Fusion Protein of S-Layer SgsE and Oxidoreductase;
Fusion Protein of S-Layer SbpA and Firefly-Luciferase
S-Layer SbpA from Lysinibacillus sphaericus with T7/lac
Part consists of the signal peptide of Erbb1/EGF-receptor, which ensures the transport to the cell membrane scFV-antiNIP, GGGSflexlinker (extracellular parts), the trans-membrane region of the Erbb1/EGF-receptor and the N-terminal part of the split-fluorophor YFP(intracellular part).
Derived by cloning of the parts BBa_K157001, BBa_K157003, BBa_K157013, BBa_K157002, BBa_K157008. For further information see the description of the parts mentioned above.
Fusion protein of S-Layer SbpA with T7/lac and BisdA and BisdB
Derived by cloning of the parts BBa_K157001, BBa_K157003, BBa_K157013, BBa_K157002 and Part one of Split- Luciferase For further information see the description of the parts mentioned above.
Second part of Split-Luciferase fused to the trans-membrane region of the Erbb1/EGF-receptor.
Derived by cloning of the parts BBa_K157002 and second part of Split-Luciferase. For further information see the description of the parts mentioned above.
Fusion Protein of S-Layer SbpA and mCerulean
Fusion Protein of BBa_K525403 and BBa_J18930
C-terminal part of the Split-fluorophore CFP, fused to the trans-membrane region of the Erbb1/EGF-receptor with the fluo-linker in between.
Derived by cloning of the parts BBa_K157002, BBa_K157009 and BBa_K157005. For further information see the description of the parts mentioned above.
S-Layer SbpA from Lysinibacillus sphaericus
Fusion Protein of mRFP, S-layer cspB from Corynebacterium halotolerans with TAT-sequence, PT7, RBS
Fusion Protein of mRFP and S-layer cspB from Corynebacterium halotolerans with TAT-sequence, PT7 and RBS
Gibson Assembly of BBa_E1010 and BBa_K525224
N-terminal part of the Split-fluorophore CFP, fused to the trans-membrane region of the Erbb1/EGF-receptor.
Derived by cloning parts BBa_K157002, BBa_K157006. For further information see the description of the parts mentioned above.
The scFv-anti-NIP (with a flexible GGGS-linker at the C-terminus) fused to the signal peptide of Erbb1/EGF-receptor.
Fusion Protein of S-Layer SgsE and mCherry RFP
Fusion Protein of BBa_K525303 and BBa_J18932