//proteindomain/head
type: Collection
persistent identity: proteindomain head
display id: proteindomain_head
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
owned by: james
owned by: myers
member: Met-His
member: c-Myc
member: HA tag
member: 7x His
member: S-tag
member: HA
member: His
member: BBa_K1909003
member: Strep-tag
member: S
member: BBa_K392005
member: FLAG
member: ErbB1 (SP)
member: V5 tag

V5 tag
type: ComponentDefinition
persistent identity: BBa T2019
display id: BBa_T2019
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: V5 epitope tag head domain (GKPIPNPLLGLDST)
creator: Reshma Shetty
created: 2009-09-02T11:00:00Z
modified: 2015-05-08T01:14:52Z
discontinued: false
dominant: false
experience: experience/None
group u list: _41_
m user id: 0
owner id: 126
owning group id: 162
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is a V5 epitope tag head domain designed to be at the N-terminus of a protein. V5 tags are a type of epitope tag that can be used to measure the quantity of proteins.
mutable notes: This head domain begins with Met-Lys to promote efficient translation of the protein in E. coli. Rare codons from various model organisms were avoided where possible.
mutable provenance: This is a synthetic sequence.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: V5 epitope tag
sequence annotation: start codon
sequence annotation: preferred AAA +2 codon
sequence: https://synbiohub.org/public/igem/BBa_T2019_sequence/1

V5 epitope tag
type: SequenceAnnotation
persistent identity: annotation2018279
display id: annotation2018279
version: 1
top level: V5 tag
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_T2019/annotation2018279/range2018279/1

https://synbiohub.org/public/igem/BBa_T2019/annotation2018279/range2018279/1
type: Range
persistent identity: range2018279
display id: range2018279
version: 1
direction: direction/forward
top level: V5 tag
start: 7
end: 48
orientation: inline

start codon
type: SequenceAnnotation
persistent identity: annotation2018277
display id: annotation2018277
version: 1
top level: V5 tag
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_T2019/annotation2018277/range2018277/1

https://synbiohub.org/public/igem/BBa_T2019/annotation2018277/range2018277/1
type: Range
persistent identity: range2018277
display id: range2018277
version: 1
direction: direction/forward
top level: V5 tag
start: 1
end: 3
orientation: inline

preferred AAA +2 codon
type: SequenceAnnotation
persistent identity: annotation2018278
display id: annotation2018278
version: 1
top level: V5 tag
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_T2019/annotation2018278/range2018278/1

https://synbiohub.org/public/igem/BBa_T2019/annotation2018278/range2018278/1
type: Range
persistent identity: range2018278
display id: range2018278
version: 1
direction: direction/forward
top level: V5 tag
start: 4
end: 6
orientation: inline

7x His
type: ComponentDefinition
persistent identity: BBa T2001
display id: BBa_T2001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: 7x His tag head domain
creator: Reshma Shetty
created: 2009-08-10T11:00:00Z
modified: 2015-05-08T01:14:52Z
discontinued: false
dominant: true
experience: experience/None
group u list: _41_
m user id: 0
owner id: 126
owning group id: 162
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is a 6x His tag head domain designed to encode the N-terminus of a protein. His tags are a type of affinity tag that can be used to purify and/or measure the quantity of proteins.
mutable notes: Both Histidine codons were used to minimize repeats. An ATG start codon was used.
mutable provenance: This is a synthetic sequence.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: preferred AAA +2 codon
sequence annotation: start codon
sequence annotation: 7x his tag
sequence: https://synbiohub.org/public/igem/BBa_T2001_sequence/1

preferred AAA +2 codon
type: SequenceAnnotation
persistent identity: annotation2017305
display id: annotation2017305
version: 1
top level: 7x His
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_T2001/annotation2017305/range2017305/1

https://synbiohub.org/public/igem/BBa_T2001/annotation2017305/range2017305/1
type: Range
persistent identity: range2017305
display id: range2017305
version: 1
direction: direction/forward
top level: 7x His
start: 4
end: 6
orientation: inline

start codon
type: SequenceAnnotation
persistent identity: annotation2016497
display id: annotation2016497
version: 1
top level: 7x His
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_T2001/annotation2016497/range2016497/1

https://synbiohub.org/public/igem/BBa_T2001/annotation2016497/range2016497/1
type: Range
persistent identity: range2016497
display id: range2016497
version: 1
direction: direction/forward
top level: 7x His
start: 1
end: 3
orientation: inline

7x his tag
type: SequenceAnnotation
persistent identity: annotation2016498
display id: annotation2016498
version: 1
top level: 7x His
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_T2001/annotation2016498/range2016498/1

https://synbiohub.org/public/igem/BBa_T2001/annotation2016498/range2016498/1
type: Range
persistent identity: range2016498
display id: range2016498
version: 1
direction: direction/forward
top level: 7x His
start: 7
end: 27
orientation: inline

BBa_K392005
type: ComponentDefinition
persistent identity: BBa K392005
display id: BBa_K392005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: yeast SUC2 leader sequence
creator: Tadashi Nakamura, Shuhei Yasumoto, Takahiro Saka, Saya Kakuda
created: 2010-10-09T11:00:00Z
modified: 2015-05-08T01:12:20Z
discontinued: false
dominant: false
experience: experience/None
group u list: _528_
m user id: 0
owner id: 6220
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: LacI promoter with RBS, followed by OmpA outer membrane protein and linker for Escherichia coli cell surface display.
mutable notes: Assembled by 3A method.
mutable provenance: Composite part BBa_K392001 (LacI promoter + RBS) and registry part BBa_K103006 (OmpA w/ linker).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence: https://synbiohub.org/public/igem/BBa_K392005_sequence/1

S
type: ComponentDefinition
persistent identity: BBa K112705
display id: BBa_K112705
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: S affinity tag
creator: Bing Xia
created: 2008-10-20T11:00:00Z
modified: 2015-05-08T01:09:18Z
discontinued: false
dominant: false
experience: experience/None
group u list: _224_
m user id: 0
owner id: 2998
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This S-tag can be added between two protein parts. ---- This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at: [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
mutable provenance: Construction of S-tag> basic part K112705 Wobble PCR dv013/dv014 (47 bp, EcoRI/BamHI/DpnI) Sub into pBca1256 (EcoRI/BamHI, 2472+47, L) Product is pBca1256-K112705 ----------------------------------------------- dv013 Forward Biobricking of S-Tag> cgataGAATTCatgAGATCTatgaaagaaaccgctgctgctaaattcg dv014 Reverse Biobricking of S-Tag> cgttaGGATCCgctgtccatgtgctggcgttcgaatttagcagcagcgg
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000324
role: partType/Tag
sequence: https://synbiohub.org/public/igem/BBa_K112705_sequence/1

ErbB1 (SP)
type: ComponentDefinition
persistent identity: BBa K157001
display id: BBa_K157001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: EGFR/ErbB1 signal peptide; mediates protein transport to a translocational pore
creator: iGEM Team Freiburg 2008
created: 2008-10-23T11:00:00Z
modified: 2015-05-08T01:10:54Z
discontinued: false
dominant: false
experience: experience/None
group u list: _232_
m user id: 0
owner id: 1673
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: The N-terminal signal-peptide ensures protein transport to the cytoplasm-membrane.
mutable notes: none
mutable provenance: Part derived from the sequence of EGFR/ErbB1
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: SP
sequence annotation: ErbB-1 Signal peptide
sequence: https://synbiohub.org/public/igem/BBa_K157001_sequence/1

SP
type: SequenceAnnotation
persistent identity: annotation2041667
display id: annotation2041667
version: 1
top level: ErbB1 (SP)
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157001/annotation2041667/range2041667/1

https://synbiohub.org/public/igem/BBa_K157001/annotation2041667/range2041667/1
type: Range
persistent identity: range2041667
display id: range2041667
version: 1
direction: direction/forward
top level: ErbB1 (SP)
start: 1
end: 72
orientation: inline

ErbB-1 Signal peptide
type: SequenceAnnotation
persistent identity: annotation2041666
display id: annotation2041666
version: 1
top level: ErbB1 (SP)
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K157001/annotation2041666/range2041666/1

https://synbiohub.org/public/igem/BBa_K157001/annotation2041666/range2041666/1
type: Range
persistent identity: range2041666
display id: range2041666
version: 1
direction: direction/forward
top level: ErbB1 (SP)
start: 1
end: 72
orientation: inline

HA tag
type: ComponentDefinition
persistent identity: BBa T2010
display id: BBa_T2010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: hemagglutinin (HA) tag head domain (YPYDVPDYA)
creator: Reshma Shetty
created: 2009-08-10T11:00:00Z
modified: 2015-05-08T01:14:52Z
discontinued: false
dominant: false
experience: experience/None
group u list: _41_
m user id: 0
owner id: 126
owning group id: 162
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is a HA tag head domain designed to be at the N-terminus of a protein. HA tags are a type of affinity tag that can be used to purify and/or measure the quantity of proteins.
mutable notes: This domain encodes the HA sequence YPYDVPDYA. The codons at each amino acid position were selected to avoid rare codons as much as possible in organisms like ''E. coli'', humans, mouse, ''B. subtilis'', ''C. elegans'', ''Streptomyces coelicolor'' and other common model organisms and/or industrial hosts. Another factor in codon selection was avoiding avoid repeat sequences.
mutable provenance: This is a synthetic sequence.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: preferred AAA +2 codon
sequence annotation: start codon
sequence annotation: HA tag
sequence: https://synbiohub.org/public/igem/BBa_T2010_sequence/1

preferred AAA +2 codon
type: SequenceAnnotation
persistent identity: annotation2017308
display id: annotation2017308
version: 1
top level: HA tag
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_T2010/annotation2017308/range2017308/1

https://synbiohub.org/public/igem/BBa_T2010/annotation2017308/range2017308/1
type: Range
persistent identity: range2017308
display id: range2017308
version: 1
direction: direction/forward
top level: HA tag
start: 4
end: 6
orientation: inline

start codon
type: SequenceAnnotation
persistent identity: annotation2017184
display id: annotation2017184
version: 1
top level: HA tag
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_T2010/annotation2017184/range2017184/1

https://synbiohub.org/public/igem/BBa_T2010/annotation2017184/range2017184/1
type: Range
persistent identity: range2017184
display id: range2017184
version: 1
direction: direction/forward
top level: HA tag
start: 1
end: 3
orientation: inline

HA tag
type: SequenceAnnotation
persistent identity: annotation2017185
display id: annotation2017185
version: 1
top level: HA tag
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_T2010/annotation2017185/range2017185/1

https://synbiohub.org/public/igem/BBa_T2010/annotation2017185/range2017185/1
type: Range
persistent identity: range2017185
display id: range2017185
version: 1
direction: direction/forward
top level: HA tag
start: 7
end: 33
orientation: inline

Met-His
type: ComponentDefinition
persistent identity: BBa K133035
display id: BBa_K133035
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Methionine-His affinity tag
creator: Jerneja Mori
created: 2008-10-25T11:00:00Z
modified: 2015-05-08T01:09:55Z
discontinued: false
dominant: false
experience: experience/None
group u list: _231_
m user id: 0
owner id: 3350
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: His-tag with amino acid Methionine.His-tag is used for detection of His-tagged proteins by anti-His antibodies.
mutable notes: Cloned into pSB1AK3 vector.
mutable provenance: Part was constructed by primer annealing.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000324
role: partType/Tag
sequence: https://synbiohub.org/public/igem/BBa_K133035_sequence/1

BBa_K1909003
type: ComponentDefinition
persistent identity: BBa K1909003
display id: BBa_K1909003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: EnvZ Signal Peptide and first Transmembrane Domain
creator: Daniel Wedemeyer
created: 2016-10-13T11:00:00Z
modified: 2016-10-14T11:06:33Z
discontinued: false
dominant: false
group u list: _2375_
m user id: 29525
owner id: 29525
owning group id: 9
status: status/Planning
bookmark: false
mutable description: For EnvZ fusion protein design. Long description will be added soon.
mutable notes: coming soon
mutable provenance: Source: E. coli EnvZ protein (ncbi: https://www.ncbi.nlm.nih.gov/gene/947272)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: EnvZ
sequence annotation: FLAG
sequence: https://synbiohub.org/public/igem/BBa_K1909003_sequence/1

EnvZ
type: SequenceAnnotation
persistent identity: annotation2507125
display id: annotation2507125
version: 1
top level: BBa_K1909003
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1909003/annotation2507125/range2507125/1

https://synbiohub.org/public/igem/BBa_K1909003/annotation2507125/range2507125/1
type: Range
persistent identity: range2507125
display id: range2507125
version: 1
direction: direction/forward
top level: BBa_K1909003
start: 1
end: 161
orientation: inline

FLAG
type: SequenceAnnotation
persistent identity: annotation2507149
display id: annotation2507149
version: 1
top level: BBa_K1909003
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1909003/annotation2507149/range2507149/1

https://synbiohub.org/public/igem/BBa_K1909003/annotation2507149/range2507149/1
type: Range
persistent identity: range2507149
display id: range2507149
version: 1
direction: direction/forward
top level: BBa_K1909003
start: 46
end: 69
orientation: inline

S-tag
type: ComponentDefinition
persistent identity: BBa T2016
display id: BBa_T2016
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: S-tag head domain (KETAAAKFERQHMDS)
creator: Reshma Shetty
created: 2009-09-02T11:00:00Z
modified: 2015-05-08T01:14:52Z
discontinued: false
dominant: false
experience: experience/None
group u list: _41_
m user id: 0
owner id: 126
owning group id: 162
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is a S-tag head domain designed to be at the N-terminus of a protein. S-tags are a type of affinity tag that can be used to purify and/or measure the quantity of proteins.
mutable notes: This head domain begins with Met-Lys to promote efficient translation of the protein in ''E. coli''.
mutable provenance: This is a synthetic sequence
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: preferred AAA +2 codon
sequence annotation: S-tag
sequence annotation: start codon
sequence: https://synbiohub.org/public/igem/BBa_T2016_sequence/1

preferred AAA +2 codon
type: SequenceAnnotation
persistent identity: annotation2018272
display id: annotation2018272
version: 1
top level: S-tag
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_T2016/annotation2018272/range2018272/1

https://synbiohub.org/public/igem/BBa_T2016/annotation2018272/range2018272/1
type: Range
persistent identity: range2018272
display id: range2018272
version: 1
direction: direction/forward
top level: S-tag
start: 4
end: 6
orientation: inline

S-tag
type: SequenceAnnotation
persistent identity: annotation2018273
display id: annotation2018273
version: 1
top level: S-tag
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_T2016/annotation2018273/range2018273/1

https://synbiohub.org/public/igem/BBa_T2016/annotation2018273/range2018273/1
type: Range
persistent identity: range2018273
display id: range2018273
version: 1
direction: direction/forward
top level: S-tag
start: 7
end: 51
orientation: inline

start codon
type: SequenceAnnotation
persistent identity: annotation2018271
display id: annotation2018271
version: 1
top level: S-tag
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_T2016/annotation2018271/range2018271/1

https://synbiohub.org/public/igem/BBa_T2016/annotation2018271/range2018271/1
type: Range
persistent identity: range2018271
display id: range2018271
version: 1
direction: direction/forward
top level: S-tag
start: 1
end: 3
orientation: inline

Strep-tag
type: ComponentDefinition
persistent identity: BBa T2013
display id: BBa_T2013
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Strep-tag II head domain (WSHPQFEK)
creator: Reshma Shetty
created: 2009-08-10T11:00:00Z
modified: 2015-05-08T01:14:52Z
discontinued: false
dominant: false
experience: experience/None
group u list: _41_
m user id: 0
owner id: 126
owning group id: 162
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is a Strep tag head domain designed to be at the N-terminus of a protein. Strep tags are typically used for detection, purification and immobilization of proteins.
mutable notes: This domain encodes the Strep tag sequence WRHPQFGG. The codons at each amino acid position were selected to avoid rare codons as much as possible in organisms like E. coli, humans, mouse, B. subtilis, C. elegans, Streptomyces coelicolor and other common model organisms and/or industrial hosts. Another factor in codon selection was avoiding avoid repeat sequences.
mutable provenance: This is a synthetic sequence.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: start codon
sequence annotation: preferred AAA +2 codon
sequence annotation: strep tag II
sequence: https://synbiohub.org/public/igem/BBa_T2013_sequence/1

start codon
type: SequenceAnnotation
persistent identity: annotation2017309
display id: annotation2017309
version: 1
top level: Strep-tag
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_T2013/annotation2017309/range2017309/1

https://synbiohub.org/public/igem/BBa_T2013/annotation2017309/range2017309/1
type: Range
persistent identity: range2017309
display id: range2017309
version: 1
direction: direction/forward
top level: Strep-tag
start: 1
end: 3
orientation: inline

preferred AAA +2 codon
type: SequenceAnnotation
persistent identity: annotation2017310
display id: annotation2017310
version: 1
top level: Strep-tag
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_T2013/annotation2017310/range2017310/1

https://synbiohub.org/public/igem/BBa_T2013/annotation2017310/range2017310/1
type: Range
persistent identity: range2017310
display id: range2017310
version: 1
direction: direction/forward
top level: Strep-tag
start: 4
end: 6
orientation: inline

strep tag II
type: SequenceAnnotation
persistent identity: annotation2017311
display id: annotation2017311
version: 1
top level: Strep-tag
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_T2013/annotation2017311/range2017311/1

https://synbiohub.org/public/igem/BBa_T2013/annotation2017311/range2017311/1
type: Range
persistent identity: range2017311
display id: range2017311
version: 1
direction: direction/forward
top level: Strep-tag
start: 7
end: 30
orientation: inline

c-Myc
type: ComponentDefinition
persistent identity: BBa I712007
display id: BBa_I712007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: c-Myc affinity tag
creator: Anja Korenčič
created: 2007-10-19T11:00:00Z
modified: 2015-08-31T04:07:45Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _130_
m user id: 0
owner id: 1846
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: c-myc tag is used for detection of proteins by anti-c-myc antibodies.
mutable notes: Gene was designed to be constructed by primer annealing. Includes start codone.
mutable provenance: Constructed by primer annealing.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000324
role: partType/Tag
sequence: https://synbiohub.org/public/igem/BBa_I712007_sequence/1

HA
type: ComponentDefinition
persistent identity: BBa I712008
display id: BBa_I712008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: HA affinity tag
creator: Anja Korenčič
created: 2007-10-19T11:00:00Z
modified: 2015-08-31T04:07:45Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _130_
m user id: 0
owner id: 1846
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: HA-tag is used for detection of HA-tagged proteins by anti-HA antibodies.
mutable notes: Gene was designed to be constructed by primer annealing. Includes start codon.
mutable provenance: Part was constructed by primer annealing.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000324
role: partType/Tag
sequence: https://synbiohub.org/public/igem/BBa_I712008_sequence/1

FLAG
type: ComponentDefinition
persistent identity: BBa T2004
display id: BBa_T2004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: FLAG octapeptide head domain (DYKDDDDK)
creator: Reshma Shetty
created: 2008-04-30T11:00:00Z
modified: 2015-05-08T01:14:52Z
discontinued: false
dominant: true
experience: experience/None
group u list: _1_
m user id: 0
owner id: 25
owning group id: 11
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Test of composite parts
mutable notes: test
mutable provenance: test
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: preferred AAA +2 codon
sequence annotation: start codon
sequence annotation: FLAG octapeptide
sequence: https://synbiohub.org/public/igem/BBa_T2004_sequence/1

preferred AAA +2 codon
type: SequenceAnnotation
persistent identity: annotation2017306
display id: annotation2017306
version: 1
top level: FLAG
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_T2004/annotation2017306/range2017306/1

https://synbiohub.org/public/igem/BBa_T2004/annotation2017306/range2017306/1
type: Range
persistent identity: range2017306
display id: range2017306
version: 1
direction: direction/forward
top level: FLAG
start: 4
end: 6
orientation: inline

start codon
type: SequenceAnnotation
persistent identity: annotation2017174
display id: annotation2017174
version: 1
top level: FLAG
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_T2004/annotation2017174/range2017174/1

https://synbiohub.org/public/igem/BBa_T2004/annotation2017174/range2017174/1
type: Range
persistent identity: range2017174
display id: range2017174
version: 1
direction: direction/forward
top level: FLAG
start: 1
end: 3
orientation: inline

FLAG octapeptide
type: SequenceAnnotation
persistent identity: annotation2017175
display id: annotation2017175
version: 1
top level: FLAG
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_T2004/annotation2017175/range2017175/1

https://synbiohub.org/public/igem/BBa_T2004/annotation2017175/range2017175/1
type: Range
persistent identity: range2017175
display id: range2017175
version: 1
direction: direction/forward
top level: FLAG
start: 7
end: 30
orientation: inline

His
type: ComponentDefinition
persistent identity: BBa K112703
display id: BBa_K112703
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: His affinity tag
creator: Bing Xia
created: 2008-10-20T11:00:00Z
modified: 2015-05-08T01:09:18Z
discontinued: false
dominant: false
experience: experience/None
group u list: _224_
m user id: 0
owner id: 2998
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This his tag can be fused to the N terminus of a protein. ---- This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at: [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
mutable provenance: Construction of His-Tag> basic part K112703 Mix dv009/dv010 (41bp, EcoRI/BamHI/DpnI) Sub into pBca1256 (EcoRI/BamHI, 2472+41, L) Product is pBca1256-K112703 ----------------------------------------------- dv009 Forward Biobricking of His-Tag> cgataGAATTCatgAGATCTatgCATCATCATCATCATCATGGATCCtaacg dv010 Reverse Biobricking of His-Tag> cgttaGGATCCATGATGATGATGATGATGcatAGATCTcatGAATTCtatgg
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000324
role: partType/Tag
sequence: https://synbiohub.org/public/igem/BBa_K112703_sequence/1

c-Myc
type: ComponentDefinition
persistent identity: BBa T2007
display id: BBa_T2007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: c-Myc head domain (EQKLISEEDL)
creator: Reshma Shetty
created: 2009-08-10T11:00:00Z
modified: 2015-05-08T01:14:52Z
discontinued: false
dominant: true
experience: experience/None
group u list: _41_
m user id: 0
owner id: 126
owning group id: 162
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is a c-Myc tag head domain designed to be at the N-terminus of a protein. c-Myc tags are a type of affinity tag that can be used to purify and/or measure the quantity of proteins.
mutable notes: This tail domain encodes the c-Myc sequence EQKLISEEDL. The codons at each amino acid position were selected to avoid rare codons in organisms like ''E. coli'', humans, mouse, ''B. subtilis'', ''C. elegans'', ''Streptomyces coelicolor'' and other common model organisms and/or industrial hosts.
mutable provenance: This is a synthetic sequence.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: preferred AAA +2 codon
sequence annotation: c-Myc tag
sequence annotation: start codon
sequence: https://synbiohub.org/public/igem/BBa_T2007_sequence/1

preferred AAA +2 codon
type: SequenceAnnotation
persistent identity: annotation2017307
display id: annotation2017307
version: 1
top level: c-Myc
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_T2007/annotation2017307/range2017307/1

https://synbiohub.org/public/igem/BBa_T2007/annotation2017307/range2017307/1
type: Range
persistent identity: range2017307
display id: range2017307
version: 1
direction: direction/forward
top level: c-Myc
start: 4
end: 6
orientation: inline

c-Myc tag
type: SequenceAnnotation
persistent identity: annotation2017180
display id: annotation2017180
version: 1
top level: c-Myc
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_T2007/annotation2017180/range2017180/1

https://synbiohub.org/public/igem/BBa_T2007/annotation2017180/range2017180/1
type: Range
persistent identity: range2017180
display id: range2017180
version: 1
direction: direction/forward
top level: c-Myc
start: 7
end: 36
orientation: inline

start codon
type: SequenceAnnotation
persistent identity: annotation2017179
display id: annotation2017179
version: 1
top level: c-Myc
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_T2007/annotation2017179/range2017179/1

https://synbiohub.org/public/igem/BBa_T2007/annotation2017179/range2017179/1
type: Range
persistent identity: range2017179
display id: range2017179
version: 1
direction: direction/forward
top level: c-Myc
start: 1
end: 3
orientation: inline