//primer/reporter/gfp
type: Collection
persistent identity: primer reporter gfp
display id: primer_reporter_gfp
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
owned by: james
owned by: myers
member: GFP-r
member: CBDcex(T7)
member: BBa_K863113
member: CBDclos(J6
member: CBDcex(J61

BBa_K863113
type: ComponentDefinition
persistent identity: BBa K863113
display id: BBa_K863113
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Cellulose binding Domain of C. cellulovorans cellulose binding protein with Reporter GFP
creator: Moritz M??ller
created: 2012-09-19T11:00:00Z
modified: 2015-06-17T01:20:29Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1123_
m user id: 4206
owner id: 13080
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Cellulose binding Domain from Clostridium cellulovorans [http://www.ncbi.nlm.nih.gov/nuccore/M73817 cellulose binding protein gene] with T7, RBS linked with two amino acids (GS) to a GFP with His-tag.
mutable notes: CBDclos fused to a his-tagged GFP in a Freiburg-manner
mutable provenance: Cloning-vector and [http://partsregistry.org/Part:BBa_K863121 BBa_K863121]
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: Cellulose binding domain (CBDclos)
sequence annotation: B0034
sequence annotation: His-Tag
sequence annotation: Cellulose Binding Protein
sequence annotation: RBS
sequence annotation: AgeI
sequence annotation: Freiburg-Scar
sequence annotation: BBa_K863111
sequence annotation: BBa_K863121
sequence annotation: TAA
sequence annotation: BBa_K863112
sequence annotation: Silent mutation (A original)
sequence annotation: T7 promoter
sequence annotation: BBa_K525998
sequence annotation: ATG
sequence annotation: BBa_E0040
sequence annotation: GS-Linker
sequence: https://synbiohub.org/public/igem/BBa_K863113_sequence/1

Cellulose binding domain (CBDclos)
type: SequenceAnnotation
persistent identity: annotation2188394
display id: annotation2188394
version: 1
top level: BBa_K863113
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K863113/annotation2188394/range2188394/1

https://synbiohub.org/public/igem/BBa_K863113/annotation2188394/range2188394/1
type: Range
persistent identity: range2188394
display id: range2188394
version: 1
direction: direction/forward
top level: BBa_K863113
start: 41
end: 316
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation2188389
display id: annotation2188389
version: 1
top level: BBa_K863113
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K863113/annotation2188389/range2188389/1

https://synbiohub.org/public/igem/BBa_K863113/annotation2188389/range2188389/1
type: Range
persistent identity: range2188389
display id: range2188389
version: 1
direction: direction/forward
top level: BBa_K863113
start: 21
end: 32
orientation: inline

His-Tag
type: SequenceAnnotation
persistent identity: annotation2188400
display id: annotation2188400
version: 1
top level: BBa_K863113
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K863113/annotation2188400/range2188400/1

https://synbiohub.org/public/igem/BBa_K863113/annotation2188400/range2188400/1
type: Range
persistent identity: range2188400
display id: range2188400
version: 1
direction: direction/forward
top level: BBa_K863113
start: 1049
end: 1066
orientation: inline

Cellulose Binding Protein
type: SequenceAnnotation
persistent identity: annotation2188392
display id: annotation2188392
version: 1
top level: BBa_K863113
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K863113/annotation2188392/range2188392/1

https://synbiohub.org/public/igem/BBa_K863113/annotation2188392/range2188392/1
type: Range
persistent identity: range2188392
display id: range2188392
version: 1
direction: direction/forward
top level: BBa_K863113
start: 35
end: 40
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2188390
display id: annotation2188390
version: 1
top level: BBa_K863113
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K863113/annotation2188390/range2188390/1

https://synbiohub.org/public/igem/BBa_K863113/annotation2188390/range2188390/1
type: Range
persistent identity: range2188390
display id: range2188390
version: 1
direction: direction/forward
top level: BBa_K863113
start: 24
end: 27
orientation: inline

AgeI
type: SequenceAnnotation
persistent identity: annotation2188401
display id: annotation2188401
version: 1
top level: BBa_K863113
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K863113/annotation2188401/range2188401/1

https://synbiohub.org/public/igem/BBa_K863113/annotation2188401/range2188401/1
type: Range
persistent identity: range2188401
display id: range2188401
version: 1
direction: direction/forward
top level: BBa_K863113
start: 1068
end: 1072
orientation: inline

Freiburg-Scar
type: SequenceAnnotation
persistent identity: annotation2188397
display id: annotation2188397
version: 1
top level: BBa_K863113
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K863113/annotation2188397/range2188397/1

https://synbiohub.org/public/igem/BBa_K863113/annotation2188397/range2188397/1
type: Range
persistent identity: range2188397
display id: range2188397
version: 1
direction: direction/forward
top level: BBa_K863113
start: 329
end: 334
orientation: inline

BBa_K863111
type: SequenceAnnotation
persistent identity: annotation2188393
display id: annotation2188393
version: 1
top level: BBa_K863113
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K863113/annotation2188393/range2188393/1

https://synbiohub.org/public/igem/BBa_K863113/annotation2188393/range2188393/1
type: Range
persistent identity: range2188393
display id: range2188393
version: 1
direction: direction/forward
top level: BBa_K863113
start: 35
end: 328
orientation: inline

BBa_K863121
type: SequenceAnnotation
persistent identity: annotation2188398
display id: annotation2188398
version: 1
top level: BBa_K863113
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K863113/annotation2188398/range2188398/1

https://synbiohub.org/public/igem/BBa_K863113/annotation2188398/range2188398/1
type: Range
persistent identity: range2188398
display id: range2188398
version: 1
direction: direction/reverse
top level: BBa_K863113
start: 335
end: 1075
orientation: inline

TAA
type: SequenceAnnotation
persistent identity: annotation2188402
display id: annotation2188402
version: 1
top level: BBa_K863113
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K863113/annotation2188402/range2188402/1

https://synbiohub.org/public/igem/BBa_K863113/annotation2188402/range2188402/1
type: Range
persistent identity: range2188402
display id: range2188402
version: 1
direction: direction/forward
top level: BBa_K863113
start: 1073
end: 1075
orientation: inline

Cellulose Binding Protein
type: SequenceAnnotation
persistent identity: annotation2188395
display id: annotation2188395
version: 1
top level: BBa_K863113
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K863113/annotation2188395/range2188395/1

https://synbiohub.org/public/igem/BBa_K863113/annotation2188395/range2188395/1
type: Range
persistent identity: range2188395
display id: range2188395
version: 1
direction: direction/forward
top level: BBa_K863113
start: 317
end: 322
orientation: inline

BBa_K863112
type: SequenceAnnotation
persistent identity: annotation2188386
display id: annotation2188386
version: 1
top level: BBa_K863113
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K863113/annotation2188386/range2188386/1

https://synbiohub.org/public/igem/BBa_K863113/annotation2188386/range2188386/1
type: Range
persistent identity: range2188386
display id: range2188386
version: 1
direction: direction/reverse
top level: BBa_K863113
start: 1
end: 328
orientation: inline

Silent mutation (A original)
type: SequenceAnnotation
persistent identity: annotation2200501
display id: annotation2200501
version: 1
top level: BBa_K863113
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_K863113/annotation2200501/range2200501/1

https://synbiohub.org/public/igem/BBa_K863113/annotation2200501/range2200501/1
type: Range
persistent identity: range2200501
display id: range2200501
version: 1
direction: direction/forward
top level: BBa_K863113
start: 112
end: 112
orientation: inline

T7 promoter
type: SequenceAnnotation
persistent identity: annotation2188388
display id: annotation2188388
version: 1
top level: BBa_K863113
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K863113/annotation2188388/range2188388/1

https://synbiohub.org/public/igem/BBa_K863113/annotation2188388/range2188388/1
type: Range
persistent identity: range2188388
display id: range2188388
version: 1
direction: direction/forward
top level: BBa_K863113
start: 1
end: 20
orientation: inline

BBa_K525998
type: SequenceAnnotation
persistent identity: annotation2188387
display id: annotation2188387
version: 1
top level: BBa_K863113
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K863113/annotation2188387/range2188387/1

https://synbiohub.org/public/igem/BBa_K863113/annotation2188387/range2188387/1
type: Range
persistent identity: range2188387
display id: range2188387
version: 1
direction: direction/forward
top level: BBa_K863113
start: 1
end: 32
orientation: inline

ATG
type: SequenceAnnotation
persistent identity: annotation2188391
display id: annotation2188391
version: 1
top level: BBa_K863113
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K863113/annotation2188391/range2188391/1

https://synbiohub.org/public/igem/BBa_K863113/annotation2188391/range2188391/1
type: Range
persistent identity: range2188391
display id: range2188391
version: 1
direction: direction/forward
top level: BBa_K863113
start: 35
end: 38
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation2188399
display id: annotation2188399
version: 1
top level: BBa_K863113
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K863113/annotation2188399/range2188399/1

https://synbiohub.org/public/igem/BBa_K863113/annotation2188399/range2188399/1
type: Range
persistent identity: range2188399
display id: range2188399
version: 1
direction: direction/forward
top level: BBa_K863113
start: 335
end: 1048
orientation: inline

GS-Linker
type: SequenceAnnotation
persistent identity: annotation2188396
display id: annotation2188396
version: 1
top level: BBa_K863113
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K863113/annotation2188396/range2188396/1

https://synbiohub.org/public/igem/BBa_K863113/annotation2188396/range2188396/1
type: Range
persistent identity: range2188396
display id: range2188396
version: 1
direction: direction/forward
top level: BBa_K863113
start: 323
end: 328
orientation: inline

GFP-r
type: ComponentDefinition
persistent identity: BBa G00201
display id: BBa_G00201
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Reverse primer for sequencing composite parts; binds to GFP (BBa_E0040)
creator: Bartholomew Canton
created: 2007-03-01T12:00:00Z
modified: 2015-08-31T04:07:28Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _11_
m user id: 0
owner id: 135
owning group id: 84
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is a reverse primer that bind about midway through the GFP coding sequence and extends towards the start codon.
mutable notes: n/a
mutable provenance: n/a
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000112
role: partType/Primer
sequence: https://synbiohub.org/public/igem/BBa_G00201_sequence/1

CBDclos(J6
type: ComponentDefinition
persistent identity: BBa K863114
display id: BBa_K863114
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CBD of C. cellulovorans cellulose binding protein gene with const. Promoter (J23100+J61101) and GFP
creator: Moritz M??ller
created: 2012-09-22T11:00:00Z
modified: 2015-06-17T01:20:44Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1123_
m user id: 4206
owner id: 13080
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Cellulose binding Domain of Clostridium cellulovorans [http://www.ncbi.nlm.nih.gov/nuccore/M73817 cellulose binding protein gene (cbp A)] with a constitutive promoter and RBS (<partinfo>J61101</partinfo>) and a fused-GFP protein with His-tag.
mutable notes: Was made to switch from a T7-promoter to a constitutive promoter
mutable provenance: Cellulose binding domain form a cloning-vector. <partinfo>J61101</partinfo> and [http://partsregistry.org/Part:BBa_I13522 GFP] from the partsregistry.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: Cellulose binding domain (CBDclos)
sequence annotation: ATG
sequence annotation: BBa_K863121
sequence annotation: TAA
sequence annotation: BBa_J23100
sequence annotation: AgeI
sequence annotation: BBa_J61101
sequence annotation: NgoMIV
sequence annotation: Cellulose Binding Protein
sequence annotation: Freiburg-Scar
sequence annotation: His-Tag
sequence annotation: BBa_E0040
sequence annotation: GS-Linker
sequence annotation: BBa_K863111
sequence: https://synbiohub.org/public/igem/BBa_K863114_sequence/1

Cellulose binding domain (CBDclos)
type: SequenceAnnotation
persistent identity: annotation2194135
display id: annotation2194135
version: 1
top level: CBDclos(J6
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K863114/annotation2194135/range2194135/1

https://synbiohub.org/public/igem/BBa_K863114/annotation2194135/range2194135/1
type: Range
persistent identity: range2194135
display id: range2194135
version: 1
direction: direction/forward
top level: CBDclos(J6
start: 83
end: 358
orientation: inline

ATG
type: SequenceAnnotation
persistent identity: annotation2194132
display id: annotation2194132
version: 1
top level: CBDclos(J6
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K863114/annotation2194132/range2194132/1

https://synbiohub.org/public/igem/BBa_K863114/annotation2194132/range2194132/1
type: Range
persistent identity: range2194132
display id: range2194132
version: 1
direction: direction/forward
top level: CBDclos(J6
start: 62
end: 64
orientation: inline

BBa_K863121
type: SequenceAnnotation
persistent identity: annotation2194139
display id: annotation2194139
version: 1
top level: CBDclos(J6
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K863114/annotation2194139/range2194139/1

https://synbiohub.org/public/igem/BBa_K863114/annotation2194139/range2194139/1
type: Range
persistent identity: range2194139
display id: range2194139
version: 1
direction: direction/reverse
top level: CBDclos(J6
start: 377
end: 1117
orientation: inline

TAA
type: SequenceAnnotation
persistent identity: annotation2194143
display id: annotation2194143
version: 1
top level: CBDclos(J6
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K863114/annotation2194143/range2194143/1

https://synbiohub.org/public/igem/BBa_K863114/annotation2194143/range2194143/1
type: Range
persistent identity: range2194143
display id: range2194143
version: 1
direction: direction/forward
top level: CBDclos(J6
start: 1115
end: 1117
orientation: inline

BBa_J23100
type: SequenceAnnotation
persistent identity: annotation2194129
display id: annotation2194129
version: 1
top level: CBDclos(J6
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K863114/annotation2194129/range2194129/1

https://synbiohub.org/public/igem/BBa_K863114/annotation2194129/range2194129/1
type: Range
persistent identity: range2194129
display id: range2194129
version: 1
direction: direction/reverse
top level: CBDclos(J6
start: 1
end: 35
orientation: inline

AgeI
type: SequenceAnnotation
persistent identity: annotation2194142
display id: annotation2194142
version: 1
top level: CBDclos(J6
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K863114/annotation2194142/range2194142/1

https://synbiohub.org/public/igem/BBa_K863114/annotation2194142/range2194142/1
type: Range
persistent identity: range2194142
display id: range2194142
version: 1
direction: direction/forward
top level: CBDclos(J6
start: 1109
end: 1114
orientation: inline

BBa_J61101
type: SequenceAnnotation
persistent identity: annotation2194130
display id: annotation2194130
version: 1
top level: CBDclos(J6
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K863114/annotation2194130/range2194130/1

https://synbiohub.org/public/igem/BBa_K863114/annotation2194130/range2194130/1
type: Range
persistent identity: range2194130
display id: range2194130
version: 1
direction: direction/reverse
top level: CBDclos(J6
start: 44
end: 55
orientation: inline

NgoMIV
type: SequenceAnnotation
persistent identity: annotation2194133
display id: annotation2194133
version: 1
top level: CBDclos(J6
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K863114/annotation2194133/range2194133/1

https://synbiohub.org/public/igem/BBa_K863114/annotation2194133/range2194133/1
type: Range
persistent identity: range2194133
display id: range2194133
version: 1
direction: direction/forward
top level: CBDclos(J6
start: 65
end: 70
orientation: inline

Cellulose Binding Protein
type: SequenceAnnotation
persistent identity: annotation2194136
display id: annotation2194136
version: 1
top level: CBDclos(J6
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K863114/annotation2194136/range2194136/1

https://synbiohub.org/public/igem/BBa_K863114/annotation2194136/range2194136/1
type: Range
persistent identity: range2194136
display id: range2194136
version: 1
direction: direction/forward
top level: CBDclos(J6
start: 359
end: 364
orientation: inline

Freiburg-Scar
type: SequenceAnnotation
persistent identity: annotation2194138
display id: annotation2194138
version: 1
top level: CBDclos(J6
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K863114/annotation2194138/range2194138/1

https://synbiohub.org/public/igem/BBa_K863114/annotation2194138/range2194138/1
type: Range
persistent identity: range2194138
display id: range2194138
version: 1
direction: direction/forward
top level: CBDclos(J6
start: 371
end: 376
orientation: inline

His-Tag
type: SequenceAnnotation
persistent identity: annotation2194141
display id: annotation2194141
version: 1
top level: CBDclos(J6
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K863114/annotation2194141/range2194141/1

https://synbiohub.org/public/igem/BBa_K863114/annotation2194141/range2194141/1
type: Range
persistent identity: range2194141
display id: range2194141
version: 1
direction: direction/forward
top level: CBDclos(J6
start: 1091
end: 1108
orientation: inline

Cellulose Binding Protein
type: SequenceAnnotation
persistent identity: annotation2194134
display id: annotation2194134
version: 1
top level: CBDclos(J6
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K863114/annotation2194134/range2194134/1

https://synbiohub.org/public/igem/BBa_K863114/annotation2194134/range2194134/1
type: Range
persistent identity: range2194134
display id: range2194134
version: 1
direction: direction/forward
top level: CBDclos(J6
start: 71
end: 82
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation2194140
display id: annotation2194140
version: 1
top level: CBDclos(J6
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K863114/annotation2194140/range2194140/1

https://synbiohub.org/public/igem/BBa_K863114/annotation2194140/range2194140/1
type: Range
persistent identity: range2194140
display id: range2194140
version: 1
direction: direction/forward
top level: CBDclos(J6
start: 377
end: 1090
orientation: inline

GS-Linker
type: SequenceAnnotation
persistent identity: annotation2194137
display id: annotation2194137
version: 1
top level: CBDclos(J6
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K863114/annotation2194137/range2194137/1

https://synbiohub.org/public/igem/BBa_K863114/annotation2194137/range2194137/1
type: Range
persistent identity: range2194137
display id: range2194137
version: 1
direction: direction/forward
top level: CBDclos(J6
start: 365
end: 370
orientation: inline

BBa_K863111
type: SequenceAnnotation
persistent identity: annotation2194131
display id: annotation2194131
version: 1
top level: CBDclos(J6
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K863114/annotation2194131/range2194131/1

https://synbiohub.org/public/igem/BBa_K863114/annotation2194131/range2194131/1
type: Range
persistent identity: range2194131
display id: range2194131
version: 1
direction: direction/reverse
top level: CBDclos(J6
start: 62
end: 370
orientation: inline

CBDcex(J61
type: ComponentDefinition
persistent identity: BBa K863104
display id: BBa_K863104
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Cellulose binding Domain from C. Fimi with const. Promotor and GFP
creator: Moritz M??ller
created: 2012-09-20T11:00:00Z
modified: 2015-06-17T01:19:39Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1123_
m user id: 4206
owner id: 13080
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Cellulose binding Domain from Cellulomonas Fimi with const. Promotor J61101 and Reporter GFP
mutable notes: a switch to a const. Promoter
mutable provenance: different
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: BBa_J61101
sequence annotation: ATG
sequence annotation: Exoglucanase
sequence annotation: AgeI
sequence annotation: NgoMIV
sequence annotation: His-Tag
sequence annotation: BBa_J23100
sequence annotation: Freiburg-Scar
sequence annotation: BBa_E0040
sequence annotation: Cellulose binding domain (CBDcex)
sequence annotation: BBa_K863121
sequence annotation: TAA
sequence annotation: BBa_K863101
sequence annotation: GS-Linker
sequence: https://synbiohub.org/public/igem/BBa_K863104_sequence/1

BBa_J61101
type: SequenceAnnotation
persistent identity: annotation2189484
display id: annotation2189484
version: 1
top level: CBDcex(J61
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K863104/annotation2189484/range2189484/1

https://synbiohub.org/public/igem/BBa_K863104/annotation2189484/range2189484/1
type: Range
persistent identity: range2189484
display id: range2189484
version: 1
direction: direction/forward
top level: CBDcex(J61
start: 44
end: 55
orientation: inline

ATG
type: SequenceAnnotation
persistent identity: annotation2189485
display id: annotation2189485
version: 1
top level: CBDcex(J61
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K863104/annotation2189485/range2189485/1

https://synbiohub.org/public/igem/BBa_K863104/annotation2189485/range2189485/1
type: Range
persistent identity: range2189485
display id: range2189485
version: 1
direction: direction/forward
top level: CBDcex(J61
start: 62
end: 64
orientation: inline

Exoglucanase
type: SequenceAnnotation
persistent identity: annotation2189488
display id: annotation2189488
version: 1
top level: CBDcex(J61
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K863104/annotation2189488/range2189488/1

https://synbiohub.org/public/igem/BBa_K863104/annotation2189488/range2189488/1
type: Range
persistent identity: range2189488
display id: range2189488
version: 1
direction: direction/forward
top level: CBDcex(J61
start: 71
end: 82
orientation: inline

AgeI
type: SequenceAnnotation
persistent identity: annotation2189496
display id: annotation2189496
version: 1
top level: CBDcex(J61
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K863104/annotation2189496/range2189496/1

https://synbiohub.org/public/igem/BBa_K863104/annotation2189496/range2189496/1
type: Range
persistent identity: range2189496
display id: range2189496
version: 1
direction: direction/forward
top level: CBDcex(J61
start: 1136
end: 1141
orientation: inline

NgoMIV
type: SequenceAnnotation
persistent identity: annotation2189487
display id: annotation2189487
version: 1
top level: CBDcex(J61
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K863104/annotation2189487/range2189487/1

https://synbiohub.org/public/igem/BBa_K863104/annotation2189487/range2189487/1
type: Range
persistent identity: range2189487
display id: range2189487
version: 1
direction: direction/forward
top level: CBDcex(J61
start: 65
end: 70
orientation: inline

His-Tag
type: SequenceAnnotation
persistent identity: annotation2189495
display id: annotation2189495
version: 1
top level: CBDcex(J61
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K863104/annotation2189495/range2189495/1

https://synbiohub.org/public/igem/BBa_K863104/annotation2189495/range2189495/1
type: Range
persistent identity: range2189495
display id: range2189495
version: 1
direction: direction/forward
top level: CBDcex(J61
start: 1118
end: 1135
orientation: inline

BBa_J23100
type: SequenceAnnotation
persistent identity: annotation2189483
display id: annotation2189483
version: 1
top level: CBDcex(J61
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K863104/annotation2189483/range2189483/1

https://synbiohub.org/public/igem/BBa_K863104/annotation2189483/range2189483/1
type: Range
persistent identity: range2189483
display id: range2189483
version: 1
direction: direction/forward
top level: CBDcex(J61
start: 1
end: 35
orientation: inline

Freiburg-Scar
type: SequenceAnnotation
persistent identity: annotation2189492
display id: annotation2189492
version: 1
top level: CBDcex(J61
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K863104/annotation2189492/range2189492/1

https://synbiohub.org/public/igem/BBa_K863104/annotation2189492/range2189492/1
type: Range
persistent identity: range2189492
display id: range2189492
version: 1
direction: direction/forward
top level: CBDcex(J61
start: 398
end: 403
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation2189494
display id: annotation2189494
version: 1
top level: CBDcex(J61
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K863104/annotation2189494/range2189494/1

https://synbiohub.org/public/igem/BBa_K863104/annotation2189494/range2189494/1
type: Range
persistent identity: range2189494
display id: range2189494
version: 1
direction: direction/forward
top level: CBDcex(J61
start: 404
end: 1117
orientation: inline

Cellulose binding domain (CBDcex)
type: SequenceAnnotation
persistent identity: annotation2189489
display id: annotation2189489
version: 1
top level: CBDcex(J61
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K863104/annotation2189489/range2189489/1

https://synbiohub.org/public/igem/BBa_K863104/annotation2189489/range2189489/1
type: Range
persistent identity: range2189489
display id: range2189489
version: 1
direction: direction/forward
top level: CBDcex(J61
start: 83
end: 382
orientation: inline

BBa_K863121
type: SequenceAnnotation
persistent identity: annotation2189493
display id: annotation2189493
version: 1
top level: CBDcex(J61
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K863104/annotation2189493/range2189493/1

https://synbiohub.org/public/igem/BBa_K863104/annotation2189493/range2189493/1
type: Range
persistent identity: range2189493
display id: range2189493
version: 1
direction: direction/forward
top level: CBDcex(J61
start: 404
end: 1144
orientation: inline

TAA
type: SequenceAnnotation
persistent identity: annotation2189497
display id: annotation2189497
version: 1
top level: CBDcex(J61
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K863104/annotation2189497/range2189497/1

https://synbiohub.org/public/igem/BBa_K863104/annotation2189497/range2189497/1
type: Range
persistent identity: range2189497
display id: range2189497
version: 1
direction: direction/forward
top level: CBDcex(J61
start: 1142
end: 1144
orientation: inline

BBa_K863101
type: SequenceAnnotation
persistent identity: annotation2189486
display id: annotation2189486
version: 1
top level: CBDcex(J61
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K863104/annotation2189486/range2189486/1

https://synbiohub.org/public/igem/BBa_K863104/annotation2189486/range2189486/1
type: Range
persistent identity: range2189486
display id: range2189486
version: 1
direction: direction/forward
top level: CBDcex(J61
start: 62
end: 397
orientation: inline

Exoglucanase
type: SequenceAnnotation
persistent identity: annotation2189490
display id: annotation2189490
version: 1
top level: CBDcex(J61
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K863104/annotation2189490/range2189490/1

https://synbiohub.org/public/igem/BBa_K863104/annotation2189490/range2189490/1
type: Range
persistent identity: range2189490
display id: range2189490
version: 1
direction: direction/forward
top level: CBDcex(J61
start: 383
end: 391
orientation: inline

GS-Linker
type: SequenceAnnotation
persistent identity: annotation2189491
display id: annotation2189491
version: 1
top level: CBDcex(J61
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K863104/annotation2189491/range2189491/1

https://synbiohub.org/public/igem/BBa_K863104/annotation2189491/range2189491/1
type: Range
persistent identity: range2189491
display id: range2189491
version: 1
direction: direction/forward
top level: CBDcex(J61
start: 392
end: 397
orientation: inline

CBDcex(T7)
type: ComponentDefinition
persistent identity: BBa K863103
display id: BBa_K863103
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Cellulose binding Domain from Cellulomonas Fimi with Reporter GFP
creator: Moritz M??ller
created: 2012-09-19T11:00:00Z
modified: 2015-06-17T01:19:26Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1123_
m user id: 4206
owner id: 13080
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Cellulose binding Domain of C. Fimi Exoglucanase gene with T7, RBS linked with two amino acids (GS) to a GFP (Freiburg-Standard)
mutable notes: CBDcex fused to a his-tagged GFP in a Freiburg-manner
mutable provenance: Cloning-vector and [http://partsregistry.org/Part:BBa_K863121 BBa_K863121]
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: His-Tag
sequence annotation: Freiburg-Scar
sequence annotation: BBa_K863121
sequence annotation: Cellulose binding domain (CBDcex)
sequence annotation: B0034
sequence annotation: GS-Linker
sequence annotation: BBa_K863101
sequence annotation: BBa_K863102
sequence annotation: T7 promoter
sequence annotation: BBa_E0040
sequence annotation: RBS
sequence annotation: TAA
sequence annotation: ATG
sequence annotation: BBa_K525998
sequence annotation: Exoglucanase
sequence annotation: AgeI
sequence: https://synbiohub.org/public/igem/BBa_K863103_sequence/1

His-Tag
type: SequenceAnnotation
persistent identity: annotation2188026
display id: annotation2188026
version: 1
top level: CBDcex(T7)
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K863103/annotation2188026/range2188026/1

https://synbiohub.org/public/igem/BBa_K863103/annotation2188026/range2188026/1
type: Range
persistent identity: range2188026
display id: range2188026
version: 1
direction: direction/forward
top level: CBDcex(T7)
start: 1085
end: 1102
orientation: inline

Freiburg-Scar
type: SequenceAnnotation
persistent identity: annotation2188023
display id: annotation2188023
version: 1
top level: CBDcex(T7)
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K863103/annotation2188023/range2188023/1

https://synbiohub.org/public/igem/BBa_K863103/annotation2188023/range2188023/1
type: Range
persistent identity: range2188023
display id: range2188023
version: 1
direction: direction/forward
top level: CBDcex(T7)
start: 365
end: 370
orientation: inline

BBa_K863121
type: SequenceAnnotation
persistent identity: annotation2188025
display id: annotation2188025
version: 1
top level: CBDcex(T7)
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K863103/annotation2188025/range2188025/1

https://synbiohub.org/public/igem/BBa_K863103/annotation2188025/range2188025/1
type: Range
persistent identity: range2188025
display id: range2188025
version: 1
direction: direction/forward
top level: CBDcex(T7)
start: 371
end: 1111
orientation: inline

Cellulose binding domain (CBDcex)
type: SequenceAnnotation
persistent identity: annotation2188020
display id: annotation2188020
version: 1
top level: CBDcex(T7)
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K863103/annotation2188020/range2188020/1

https://synbiohub.org/public/igem/BBa_K863103/annotation2188020/range2188020/1
type: Range
persistent identity: range2188020
display id: range2188020
version: 1
direction: direction/forward
top level: CBDcex(T7)
start: 50
end: 349
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation2188015
display id: annotation2188015
version: 1
top level: CBDcex(T7)
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K863103/annotation2188015/range2188015/1

https://synbiohub.org/public/igem/BBa_K863103/annotation2188015/range2188015/1
type: Range
persistent identity: range2188015
display id: range2188015
version: 1
direction: direction/forward
top level: CBDcex(T7)
start: 21
end: 32
orientation: inline

GS-Linker
type: SequenceAnnotation
persistent identity: annotation2188022
display id: annotation2188022
version: 1
top level: CBDcex(T7)
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K863103/annotation2188022/range2188022/1

https://synbiohub.org/public/igem/BBa_K863103/annotation2188022/range2188022/1
type: Range
persistent identity: range2188022
display id: range2188022
version: 1
direction: direction/forward
top level: CBDcex(T7)
start: 359
end: 364
orientation: inline

BBa_K863101
type: SequenceAnnotation
persistent identity: annotation2188019
display id: annotation2188019
version: 1
top level: CBDcex(T7)
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K863103/annotation2188019/range2188019/1

https://synbiohub.org/public/igem/BBa_K863103/annotation2188019/range2188019/1
type: Range
persistent identity: range2188019
display id: range2188019
version: 1
direction: direction/forward
top level: CBDcex(T7)
start: 38
end: 364
orientation: inline

BBa_K863102
type: SequenceAnnotation
persistent identity: annotation2188014
display id: annotation2188014
version: 1
top level: CBDcex(T7)
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K863103/annotation2188014/range2188014/1

https://synbiohub.org/public/igem/BBa_K863103/annotation2188014/range2188014/1
type: Range
persistent identity: range2188014
display id: range2188014
version: 1
direction: direction/forward
top level: CBDcex(T7)
start: 1
end: 364
orientation: inline

T7 promoter
type: SequenceAnnotation
persistent identity: annotation2188012
display id: annotation2188012
version: 1
top level: CBDcex(T7)
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K863103/annotation2188012/range2188012/1

https://synbiohub.org/public/igem/BBa_K863103/annotation2188012/range2188012/1
type: Range
persistent identity: range2188012
display id: range2188012
version: 1
direction: direction/forward
top level: CBDcex(T7)
start: 1
end: 20
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation2188024
display id: annotation2188024
version: 1
top level: CBDcex(T7)
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K863103/annotation2188024/range2188024/1

https://synbiohub.org/public/igem/BBa_K863103/annotation2188024/range2188024/1
type: Range
persistent identity: range2188024
display id: range2188024
version: 1
direction: direction/forward
top level: CBDcex(T7)
start: 371
end: 1084
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2188016
display id: annotation2188016
version: 1
top level: CBDcex(T7)
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K863103/annotation2188016/range2188016/1

https://synbiohub.org/public/igem/BBa_K863103/annotation2188016/range2188016/1
type: Range
persistent identity: range2188016
display id: range2188016
version: 1
direction: direction/forward
top level: CBDcex(T7)
start: 24
end: 27
orientation: inline

TAA
type: SequenceAnnotation
persistent identity: annotation2188028
display id: annotation2188028
version: 1
top level: CBDcex(T7)
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K863103/annotation2188028/range2188028/1

https://synbiohub.org/public/igem/BBa_K863103/annotation2188028/range2188028/1
type: Range
persistent identity: range2188028
display id: range2188028
version: 1
direction: direction/forward
top level: CBDcex(T7)
start: 1109
end: 1111
orientation: inline

ATG
type: SequenceAnnotation
persistent identity: annotation2188017
display id: annotation2188017
version: 1
top level: CBDcex(T7)
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K863103/annotation2188017/range2188017/1

https://synbiohub.org/public/igem/BBa_K863103/annotation2188017/range2188017/1
type: Range
persistent identity: range2188017
display id: range2188017
version: 1
direction: direction/forward
top level: CBDcex(T7)
start: 35
end: 37
orientation: inline

BBa_K525998
type: SequenceAnnotation
persistent identity: annotation2188013
display id: annotation2188013
version: 1
top level: CBDcex(T7)
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K863103/annotation2188013/range2188013/1

https://synbiohub.org/public/igem/BBa_K863103/annotation2188013/range2188013/1
type: Range
persistent identity: range2188013
display id: range2188013
version: 1
direction: direction/forward
top level: CBDcex(T7)
start: 1
end: 32
orientation: inline

Exoglucanase
type: SequenceAnnotation
persistent identity: annotation2188018
display id: annotation2188018
version: 1
top level: CBDcex(T7)
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K863103/annotation2188018/range2188018/1

https://synbiohub.org/public/igem/BBa_K863103/annotation2188018/range2188018/1
type: Range
persistent identity: range2188018
display id: range2188018
version: 1
direction: direction/forward
top level: CBDcex(T7)
start: 38
end: 49
orientation: inline

Exoglucanase
type: SequenceAnnotation
persistent identity: annotation2188021
display id: annotation2188021
version: 1
top level: CBDcex(T7)
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K863103/annotation2188021/range2188021/1

https://synbiohub.org/public/igem/BBa_K863103/annotation2188021/range2188021/1
type: Range
persistent identity: range2188021
display id: range2188021
version: 1
direction: direction/forward
top level: CBDcex(T7)
start: 350
end: 358
orientation: inline

AgeI
type: SequenceAnnotation
persistent identity: annotation2188027
display id: annotation2188027
version: 1
top level: CBDcex(T7)
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K863103/annotation2188027/range2188027/1

https://synbiohub.org/public/igem/BBa_K863103/annotation2188027/range2188027/1
type: Range
persistent identity: range2188027
display id: range2188027
version: 1
direction: direction/forward
top level: CBDcex(T7)
start: 1103
end: 1108
orientation: inline