//plasmidbackbone/version/_3
type: Collection
persistent identity: plasmidbackbone version 3
display id: plasmidbackbone_version__3
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
owned by: james
owned by: myers
member: pSB4A3
member: pSB1A30
member: pSB2K3
member: pSB3K3

pSB1A30
type: ComponentDefinition
persistent identity: BBa K1362091
display id: BBa_K1362091
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pSB1A30: High copy BioBrick cloning/expression backbone carrying Amp resitance
creator: Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena B??scher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Sch??fer, Carolin Schmelas, Silvan Schmitz, Max Waldha
created: 2014-10-07T11:00:00Z
modified: 2015-05-08T01:10:04Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1738_
m user id: 0
owner id: 22830
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: VR
sequence annotation: terminator stem loop
sequence annotation: E. coli his operon terminator
sequence annotation: ORI
sequence annotation: end of terminator
sequence annotation: T7 promotor
sequence annotation: ampR (rev)
sequence annotation: prefix
sequence annotation: suffix
sequence annotation: lacO reg
sequence annotation: rep(pMB1) (rev)
sequence annotation: VF2
sequence: https://synbiohub.org/public/igem/BBa_K1362091_sequence/1

VR
type: SequenceAnnotation
persistent identity: annotation2404602
display id: annotation2404602
version: 1
top level: pSB1A30
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K1362091/annotation2404602/range2404602/1

https://synbiohub.org/public/igem/BBa_K1362091/annotation2404602/range2404602/1
type: Range
persistent identity: range2404602
display id: range2404602
version: 1
direction: direction/reverse
top level: pSB1A30
start: 157
end: 176
orientation: inline

terminator stem loop
type: SequenceAnnotation
persistent identity: annotation2404610
display id: annotation2404610
version: 1
top level: pSB1A30
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1362091/annotation2404610/range2404610/1

https://synbiohub.org/public/igem/BBa_K1362091/annotation2404610/range2404610/1
type: Range
persistent identity: range2404610
display id: range2404610
version: 1
direction: direction/forward
top level: pSB1A30
start: 2107
end: 2126
orientation: inline

E. coli his operon terminator
type: SequenceAnnotation
persistent identity: annotation2404601
display id: annotation2404601
version: 1
top level: pSB1A30
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1362091/annotation2404601/range2404601/1

https://synbiohub.org/public/igem/BBa_K1362091/annotation2404601/range2404601/1
type: Range
persistent identity: range2404601
display id: range2404601
version: 1
direction: direction/forward
top level: pSB1A30
start: 22
end: 93
orientation: inline

ORI
type: SequenceAnnotation
persistent identity: annotation2404606
display id: annotation2404606
version: 1
top level: pSB1A30
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1362091/annotation2404606/range2404606/1

https://synbiohub.org/public/igem/BBa_K1362091/annotation2404606/range2404606/1
type: Range
persistent identity: range2404606
display id: range2404606
version: 1
direction: direction/forward
top level: pSB1A30
start: 276
end: 276
orientation: inline

end of terminator
type: SequenceAnnotation
persistent identity: annotation2404609
display id: annotation2404609
version: 1
top level: pSB1A30
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1362091/annotation2404609/range2404609/1

https://synbiohub.org/public/igem/BBa_K1362091/annotation2404609/range2404609/1
type: Range
persistent identity: range2404609
display id: range2404609
version: 1
direction: direction/reverse
top level: pSB1A30
start: 2094
end: 2097
orientation: inline

T7 promotor
type: SequenceAnnotation
persistent identity: annotation2404611
display id: annotation2404611
version: 1
top level: pSB1A30
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1362091/annotation2404611/range2404611/1

https://synbiohub.org/public/igem/BBa_K1362091/annotation2404611/range2404611/1
type: Range
persistent identity: range2404611
display id: range2404611
version: 1
direction: direction/forward
top level: pSB1A30
start: 2139
end: 2157
orientation: inline

ampR (rev)
type: SequenceAnnotation
persistent identity: annotation2404607
display id: annotation2404607
version: 1
top level: pSB1A30
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1362091/annotation2404607/range2404607/1

https://synbiohub.org/public/igem/BBa_K1362091/annotation2404607/range2404607/1
type: Range
persistent identity: range2404607
display id: range2404607
version: 1
direction: direction/reverse
top level: pSB1A30
start: 1035
end: 1895
orientation: inline

prefix
type: SequenceAnnotation
persistent identity: annotation2404632
display id: annotation2404632
version: 1
top level: pSB1A30
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1362091/annotation2404632/range2404632/1

https://synbiohub.org/public/igem/BBa_K1362091/annotation2404632/range2404632/1
type: Range
persistent identity: range2404632
display id: range2404632
version: 1
direction: direction/forward
top level: pSB1A30
start: 2185
end: 2206
orientation: inline

suffix
type: SequenceAnnotation
persistent identity: annotation2404600
display id: annotation2404600
version: 1
top level: pSB1A30
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1362091/annotation2404600/range2404600/1

https://synbiohub.org/public/igem/BBa_K1362091/annotation2404600/range2404600/1
type: Range
persistent identity: range2404600
display id: range2404600
version: 1
direction: direction/forward
top level: pSB1A30
start: 1
end: 21
orientation: inline

terminator stem loop
type: SequenceAnnotation
persistent identity: annotation2404603
display id: annotation2404603
version: 1
top level: pSB1A30
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1362091/annotation2404603/range2404603/1

https://synbiohub.org/public/igem/BBa_K1362091/annotation2404603/range2404603/1
type: Range
persistent identity: range2404603
display id: range2404603
version: 1
direction: direction/forward
top level: pSB1A30
start: 30
end: 64
orientation: inline

lacO reg
type: SequenceAnnotation
persistent identity: annotation2404631
display id: annotation2404631
version: 1
top level: pSB1A30
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K1362091/annotation2404631/range2404631/1

https://synbiohub.org/public/igem/BBa_K1362091/annotation2404631/range2404631/1
type: Range
persistent identity: range2404631
display id: range2404631
version: 1
direction: direction/forward
top level: pSB1A30
start: 2157
end: 2184
orientation: inline

rep(pMB1) (rev)
type: SequenceAnnotation
persistent identity: annotation2404605
display id: annotation2404605
version: 1
top level: pSB1A30
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1362091/annotation2404605/range2404605/1

https://synbiohub.org/public/igem/BBa_K1362091/annotation2404605/range2404605/1
type: Range
persistent identity: range2404605
display id: range2404605
version: 1
direction: direction/reverse
top level: pSB1A30
start: 261
end: 875
orientation: inline

VF2
type: SequenceAnnotation
persistent identity: annotation2404608
display id: annotation2404608
version: 1
top level: pSB1A30
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K1362091/annotation2404608/range2404608/1

https://synbiohub.org/public/igem/BBa_K1362091/annotation2404608/range2404608/1
type: Range
persistent identity: range2404608
display id: range2404608
version: 1
direction: direction/forward
top level: pSB1A30
start: 2018
end: 2037
orientation: inline

pSB4A3
type: ComponentDefinition
persistent identity: pSB4A3
display id: pSB4A3
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pSB4A3 (Replaced by pSB4A5)
creator: Reshma Shetty
created: 2004-06-28T11:00:00Z
modified: 2015-05-08T01:14:55Z
discontinued: false
dominant: false
experience: experience/Works
m user id: 0
owner id: 60
owning group id: 7
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: pSB4A3-1 is a low copy plasmid with ampicillin resistance. It has a repA pSC101-derived replication origin. (Copy number ~10-12, Lutz and Bujard, 1997.) pSB4A3 has a terminator upstream of its MCS, which is oriented to prevent transcription from *inside* the MCS from reading out into any DNA outside the MCS.The orientation of the pBLA promoter driving the AmpR gene is such that relatively little transcription should be coming into the MCS from upstream. A second terminator (E.coli His operon-derived) is downstream of the MCS, again insulating the vector from transcription reading out of the MCS. Ideally, future versions of standard biobrick vectors would have terminators bracketing the MCS that were 100% efficient in terminating transcription both into and out of the MCS region.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: repA (rev)
sequence annotation: E.coli His operon terminator
sequence annotation: AmpR (rev)
sequence annotation: suffix
sequence annotation: -35
sequence annotation: VF2
sequence annotation: terminator stem loop
sequence annotation: VR
sequence annotation: rbs(rev)
sequence annotation: -10
sequence annotation: RBS (rev)
sequence annotation: end of terminator
sequence annotation: deletion of two bases
sequence annotation: prefix
sequence annotation: (rev)
sequence annotation: Terminator (rev)
sequence: https://synbiohub.org/public/igem/pSB4A3_sequence/1

repA (rev)
type: SequenceAnnotation
persistent identity: annotation1782534
display id: annotation1782534
version: 1
top level: pSB4A3
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/pSB4A3/annotation1782534/range1782534/1

https://synbiohub.org/public/igem/pSB4A3/annotation1782534/range1782534/1
type: Range
persistent identity: range1782534
display id: range1782534
version: 1
direction: direction/reverse
top level: pSB4A3
start: 384
end: 1334
orientation: inline

E.coli His operon terminator
type: SequenceAnnotation
persistent identity: annotation1782533
display id: annotation1782533
version: 1
top level: pSB4A3
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB4A3/annotation1782533/range1782533/1

https://synbiohub.org/public/igem/pSB4A3/annotation1782533/range1782533/1
type: Range
persistent identity: range1782533
display id: range1782533
version: 1
direction: direction/forward
top level: pSB4A3
start: 22
end: 93
orientation: inline

AmpR (rev)
type: SequenceAnnotation
persistent identity: annotation1782537
display id: annotation1782537
version: 1
top level: pSB4A3
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/pSB4A3/annotation1782537/range1782537/1

https://synbiohub.org/public/igem/pSB4A3/annotation1782537/range1782537/1
type: Range
persistent identity: range1782537
display id: range1782537
version: 1
direction: direction/reverse
top level: pSB4A3
start: 2220
end: 3080
orientation: inline

suffix
type: SequenceAnnotation
persistent identity: annotation1782532
display id: annotation1782532
version: 1
top level: pSB4A3
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/pSB4A3/annotation1782532/range1782532/1

https://synbiohub.org/public/igem/pSB4A3/annotation1782532/range1782532/1
type: Range
persistent identity: range1782532
display id: range1782532
version: 1
direction: direction/forward
top level: pSB4A3
start: 1
end: 21
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1782529
display id: annotation1782529
version: 1
top level: pSB4A3
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/pSB4A3/annotation1782529/range1782529/1

https://synbiohub.org/public/igem/pSB4A3/annotation1782529/range1782529/1
type: Range
persistent identity: range1782529
display id: range1782529
version: 1
direction: direction/forward
top level: pSB4A3
start: 3124
end: 3129
orientation: inline

VF2
type: SequenceAnnotation
persistent identity: annotation1782550
display id: annotation1782550
version: 1
top level: pSB4A3
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/pSB4A3/annotation1782550/range1782550/1

https://synbiohub.org/public/igem/pSB4A3/annotation1782550/range1782550/1
type: Range
persistent identity: range1782550
display id: range1782550
version: 1
direction: direction/forward
top level: pSB4A3
start: 3200
end: 3219
orientation: inline

terminator stem loop
type: SequenceAnnotation
persistent identity: annotation1824032
display id: annotation1824032
version: 1
top level: pSB4A3
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/pSB4A3/annotation1824032/range1824032/1

https://synbiohub.org/public/igem/pSB4A3/annotation1824032/range1824032/1
type: Range
persistent identity: range1824032
display id: range1824032
version: 1
direction: direction/reverse
top level: pSB4A3
start: 3289
end: 3308
orientation: inline

VR
type: SequenceAnnotation
persistent identity: annotation1782542
display id: annotation1782542
version: 1
top level: pSB4A3
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/pSB4A3/annotation1782542/range1782542/1

https://synbiohub.org/public/igem/pSB4A3/annotation1782542/range1782542/1
type: Range
persistent identity: range1782542
display id: range1782542
version: 1
direction: direction/reverse
top level: pSB4A3
start: 157
end: 176
orientation: inline

rbs(rev)
type: SequenceAnnotation
persistent identity: annotation1782527
display id: annotation1782527
version: 1
top level: pSB4A3
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/pSB4A3/annotation1782527/range1782527/1

https://synbiohub.org/public/igem/pSB4A3/annotation1782527/range1782527/1
type: Range
persistent identity: range1782527
display id: range1782527
version: 1
direction: direction/reverse
top level: pSB4A3
start: 3085
end: 3089
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1782528
display id: annotation1782528
version: 1
top level: pSB4A3
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/pSB4A3/annotation1782528/range1782528/1

https://synbiohub.org/public/igem/pSB4A3/annotation1782528/range1782528/1
type: Range
persistent identity: range1782528
display id: range1782528
version: 1
direction: direction/forward
top level: pSB4A3
start: 3098
end: 3103
orientation: inline

RBS (rev)
type: SequenceAnnotation
persistent identity: annotation1782535
display id: annotation1782535
version: 1
top level: pSB4A3
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/pSB4A3/annotation1782535/range1782535/1

https://synbiohub.org/public/igem/pSB4A3/annotation1782535/range1782535/1
type: Range
persistent identity: range1782535
display id: range1782535
version: 1
direction: direction/reverse
top level: pSB4A3
start: 1338
end: 1350
orientation: inline

end of terminator
type: SequenceAnnotation
persistent identity: annotation1782530
display id: annotation1782530
version: 1
top level: pSB4A3
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB4A3/annotation1782530/range1782530/1

https://synbiohub.org/public/igem/pSB4A3/annotation1782530/range1782530/1
type: Range
persistent identity: range1782530
display id: range1782530
version: 1
direction: direction/reverse
top level: pSB4A3
start: 3276
end: 3279
orientation: inline

deletion of two bases
type: SequenceAnnotation
persistent identity: annotation1823771
display id: annotation1823771
version: 1
top level: pSB4A3
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB4A3/annotation1823771/range1823771/1

https://synbiohub.org/public/igem/pSB4A3/annotation1823771/range1823771/1
type: Range
persistent identity: range1823771
display id: range1823771
version: 1
direction: direction/forward
top level: pSB4A3
start: 34
end: 35
orientation: inline

prefix
type: SequenceAnnotation
persistent identity: annotation1782531
display id: annotation1782531
version: 1
top level: pSB4A3
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/pSB4A3/annotation1782531/range1782531/1

https://synbiohub.org/public/igem/pSB4A3/annotation1782531/range1782531/1
type: Range
persistent identity: range1782531
display id: range1782531
version: 1
direction: direction/forward
top level: pSB4A3
start: 3318
end: 3339
orientation: inline

(rev)
type: SequenceAnnotation
persistent identity: annotation1782536
display id: annotation1782536
version: 1
top level: pSB4A3
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/pSB4A3/annotation1782536/range1782536/1

https://synbiohub.org/public/igem/pSB4A3/annotation1782536/range1782536/1
type: Range
persistent identity: range1782536
display id: range1782536
version: 1
direction: direction/reverse
top level: pSB4A3
start: 1355
end: 1374
orientation: inline

Terminator (rev)
type: SequenceAnnotation
persistent identity: annotation1782538
display id: annotation1782538
version: 1
top level: pSB4A3
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/pSB4A3/annotation1782538/range1782538/1

https://synbiohub.org/public/igem/pSB4A3/annotation1782538/range1782538/1
type: Range
persistent identity: range1782538
display id: range1782538
version: 1
direction: direction/reverse
top level: pSB4A3
start: 2145
end: 2174
orientation: inline

terminator stem loop
type: SequenceAnnotation
persistent identity: annotation1824031
display id: annotation1824031
version: 1
top level: pSB4A3
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/pSB4A3/annotation1824031/range1824031/1

https://synbiohub.org/public/igem/pSB4A3/annotation1824031/range1824031/1
type: Range
persistent identity: range1824031
display id: range1824031
version: 1
direction: direction/forward
top level: pSB4A3
start: 30
end: 64
orientation: inline

pSB3K3
type: ComponentDefinition
persistent identity: pSB3K3
display id: pSB3K3
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Low to medium copy BioBrick standard vector
creator: Reshma Shetty
created: 2004-06-28T11:00:00Z
modified: 2015-05-08T01:14:55Z
discontinued: false
dominant: false
experience: experience/Works
m user id: 0
owner id: 60
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: pSB3K3-1 is a medium copy plasmid with kanamycin resistance. It has a p15A pMR101-derived replication origin, copy number 20-30. (Lutz and Bujard, 1997) pSB3K3-1 has a terminator upstream of its MCS, which is oriented to prevent transcription from *inside* the MCS from reading out into the vectorl. A second terminator (E.coli His operon-derived) is downstream of the MCS, again insulating the vector from transcription reading out of the MCS. Ideally, future versions of standard biobrick vectors would have terminators bracketing the MCS that were 100% efficient in terminating transcription both into and out of the MCS region.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: E. coli his operon terminator
sequence annotation: terminator stem loop
sequence annotation: p15A ORI (rev)
sequence annotation: Suffix
sequence annotation: end of terminator
sequence annotation: Prefix
sequence annotation: VR
sequence annotation: VF2
sequence annotation: kanR (rev)
sequence annotation: deletion of two bases
sequence: https://synbiohub.org/public/igem/pSB3K3_sequence/1

E. coli his operon terminator
type: SequenceAnnotation
persistent identity: annotation1824021
display id: annotation1824021
version: 1
top level: pSB3K3
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB3K3/annotation1824021/range1824021/1

https://synbiohub.org/public/igem/pSB3K3/annotation1824021/range1824021/1
type: Range
persistent identity: range1824021
display id: range1824021
version: 1
direction: direction/forward
top level: pSB3K3
start: 22
end: 93
orientation: inline

terminator stem loop
type: SequenceAnnotation
persistent identity: annotation1824022
display id: annotation1824022
version: 1
top level: pSB3K3
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/pSB3K3/annotation1824022/range1824022/1

https://synbiohub.org/public/igem/pSB3K3/annotation1824022/range1824022/1
type: Range
persistent identity: range1824022
display id: range1824022
version: 1
direction: direction/forward
top level: pSB3K3
start: 30
end: 64
orientation: inline

p15A ORI (rev)
type: SequenceAnnotation
persistent identity: annotation1023046
display id: annotation1023046
version: 1
top level: pSB3K3
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/pSB3K3/annotation1023046/range1023046/1

https://synbiohub.org/public/igem/pSB3K3/annotation1023046/range1023046/1
type: Range
persistent identity: range1023046
display id: range1023046
version: 1
direction: direction/reverse
top level: pSB3K3
start: 1386
end: 2210
orientation: inline

Suffix
type: SequenceAnnotation
persistent identity: annotation1023044
display id: annotation1023044
version: 1
top level: pSB3K3
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/pSB3K3/annotation1023044/range1023044/1

https://synbiohub.org/public/igem/pSB3K3/annotation1023044/range1023044/1
type: Range
persistent identity: range1023044
display id: range1023044
version: 1
direction: direction/forward
top level: pSB3K3
start: 1
end: 21
orientation: inline

end of terminator
type: SequenceAnnotation
persistent identity: annotation1824024
display id: annotation1824024
version: 1
top level: pSB3K3
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB3K3/annotation1824024/range1824024/1

https://synbiohub.org/public/igem/pSB3K3/annotation1824024/range1824024/1
type: Range
persistent identity: range1824024
display id: range1824024
version: 1
direction: direction/reverse
top level: pSB3K3
start: 2687
end: 2690
orientation: inline

terminator stem loop
type: SequenceAnnotation
persistent identity: annotation1824023
display id: annotation1824023
version: 1
top level: pSB3K3
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/pSB3K3/annotation1824023/range1824023/1

https://synbiohub.org/public/igem/pSB3K3/annotation1824023/range1824023/1
type: Range
persistent identity: range1824023
display id: range1824023
version: 1
direction: direction/reverse
top level: pSB3K3
start: 2700
end: 2719
orientation: inline

Prefix
type: SequenceAnnotation
persistent identity: annotation1023043
display id: annotation1023043
version: 1
top level: pSB3K3
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/pSB3K3/annotation1023043/range1023043/1

https://synbiohub.org/public/igem/pSB3K3/annotation1023043/range1023043/1
type: Range
persistent identity: range1023043
display id: range1023043
version: 1
direction: direction/forward
top level: pSB3K3
start: 2729
end: 2750
orientation: inline

VR
type: SequenceAnnotation
persistent identity: annotation1782476
display id: annotation1782476
version: 1
top level: pSB3K3
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/pSB3K3/annotation1782476/range1782476/1

https://synbiohub.org/public/igem/pSB3K3/annotation1782476/range1782476/1
type: Range
persistent identity: range1782476
display id: range1782476
version: 1
direction: direction/reverse
top level: pSB3K3
start: 157
end: 176
orientation: inline

VF2
type: SequenceAnnotation
persistent identity: annotation1782459
display id: annotation1782459
version: 1
top level: pSB3K3
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/pSB3K3/annotation1782459/range1782459/1

https://synbiohub.org/public/igem/pSB3K3/annotation1782459/range1782459/1
type: Range
persistent identity: range1782459
display id: range1782459
version: 1
direction: direction/forward
top level: pSB3K3
start: 2611
end: 2630
orientation: inline

kanR (rev)
type: SequenceAnnotation
persistent identity: annotation1023045
display id: annotation1023045
version: 1
top level: pSB3K3
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/pSB3K3/annotation1023045/range1023045/1

https://synbiohub.org/public/igem/pSB3K3/annotation1023045/range1023045/1
type: Range
persistent identity: range1023045
display id: range1023045
version: 1
direction: direction/reverse
top level: pSB3K3
start: 239
end: 1054
orientation: inline

deletion of two bases
type: SequenceAnnotation
persistent identity: annotation1823788
display id: annotation1823788
version: 1
top level: pSB3K3
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB3K3/annotation1823788/range1823788/1

https://synbiohub.org/public/igem/pSB3K3/annotation1823788/range1823788/1
type: Range
persistent identity: range1823788
display id: range1823788
version: 1
direction: direction/forward
top level: pSB3K3
start: 34
end: 35
orientation: inline

pSB2K3
type: ComponentDefinition
persistent identity: pSB2K3
display id: pSB2K3
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Inducible copy number BioBrick plasmid
creator: Sri Kosuri
created: 2004-05-03T11:00:00Z
modified: 2015-05-08T01:14:55Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _11_48_46_1_
m user id: 0
owner id: 52
owning group id: 7
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: pSB2K3 is a variable copy number plasmid carrying kanamycin resistance. The plasmid contains the F' replication origin and also the P1 lytic replication origin. Genes encoding proteins that are required to activate the P1 lytic replication origin are carried on the plasmid under the control of a lacI-regulated promoter. When grown in cells producing LacI the plasmid is maintained at very low copy (<10 per cell). When induced with IPTG the P1 lytic replication proteins are produced and the P1 lytic replication origin takes over, raising the copy number to very high levels (>100++ per cell). The copy control features of pSB2K3 are useful for cloning "difficult" parts while still being able to prepare large amounts of DNA. Additionally, pSB2K3 has terminators bracketing its MCS which are designed to prevent transcription from *inside* the MCS from reading out into the vector. The efficiency of these terminators is known to be < 100%. Ideally we would construct a future set of terminators for bracketing a MCS that were 100% efficient in terminating both into and out of the MCS region.
mutable notes: *** pSCANS via BNL *** The pSB2 series plasmids are based on the pSCANS-5 plasmid from John Dunn's group at Brookhaven National Laboratory. The pSCANS plasmids are introduced here.
mutable provenance: MIT SBWG modification of pSCANS-5 from BNL
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: terminator stem loop
sequence annotation: E. coli his operon terminator
sequence annotation: end of terminator
sequence annotation: repE (rev)
sequence annotation: lacOP
sequence annotation: repL
sequence annotation: nnpp promoters
sequence annotation: kanR
sequence annotation: prefix
sequence annotation: F1 Replication Origin
sequence annotation: oriS Replication Origin (rev)
sequence annotation: suffix
sequence annotation: VF2
sequence annotation: deletion of two bases
sequence annotation: VR
sequence: https://synbiohub.org/public/igem/pSB2K3_sequence/1

terminator stem loop
type: SequenceAnnotation
persistent identity: annotation1824016
display id: annotation1824016
version: 1
top level: pSB2K3
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/pSB2K3/annotation1824016/range1824016/1

https://synbiohub.org/public/igem/pSB2K3/annotation1824016/range1824016/1
type: Range
persistent identity: range1824016
display id: range1824016
version: 1
direction: direction/reverse
top level: pSB2K3
start: 4375
end: 4394
orientation: inline

E. coli his operon terminator
type: SequenceAnnotation
persistent identity: annotation1824015
display id: annotation1824015
version: 1
top level: pSB2K3
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB2K3/annotation1824015/range1824015/1

https://synbiohub.org/public/igem/pSB2K3/annotation1824015/range1824015/1
type: Range
persistent identity: range1824015
display id: range1824015
version: 1
direction: direction/forward
top level: pSB2K3
start: 22
end: 93
orientation: inline

end of terminator
type: SequenceAnnotation
persistent identity: annotation1824017
display id: annotation1824017
version: 1
top level: pSB2K3
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB2K3/annotation1824017/range1824017/1

https://synbiohub.org/public/igem/pSB2K3/annotation1824017/range1824017/1
type: Range
persistent identity: range1824017
display id: range1824017
version: 1
direction: direction/reverse
top level: pSB2K3
start: 4362
end: 4365
orientation: inline

repE (rev)
type: SequenceAnnotation
persistent identity: annotation1023024
display id: annotation1023024
version: 1
top level: pSB2K3
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/pSB2K3/annotation1023024/range1023024/1

https://synbiohub.org/public/igem/pSB2K3/annotation1023024/range1023024/1
type: Range
persistent identity: range1023024
display id: range1023024
version: 1
direction: direction/reverse
top level: pSB2K3
start: 617
end: 1372
orientation: inline

lacOP
type: SequenceAnnotation
persistent identity: annotation1023026
display id: annotation1023026
version: 1
top level: pSB2K3
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/pSB2K3/annotation1023026/range1023026/1

https://synbiohub.org/public/igem/pSB2K3/annotation1023026/range1023026/1
type: Range
persistent identity: range1023026
display id: range1023026
version: 1
direction: direction/forward
top level: pSB2K3
start: 2037
end: 2211
orientation: inline

repL
type: SequenceAnnotation
persistent identity: annotation1023027
display id: annotation1023027
version: 1
top level: pSB2K3
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/pSB2K3/annotation1023027/range1023027/1

https://synbiohub.org/public/igem/pSB2K3/annotation1023027/range1023027/1
type: Range
persistent identity: range1023027
display id: range1023027
version: 1
direction: direction/forward
top level: pSB2K3
start: 2289
end: 3134
orientation: inline

nnpp promoters
type: SequenceAnnotation
persistent identity: annotation1023028
display id: annotation1023028
version: 1
top level: pSB2K3
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/pSB2K3/annotation1023028/range1023028/1

https://synbiohub.org/public/igem/pSB2K3/annotation1023028/range1023028/1
type: Range
persistent identity: range1023028
display id: range1023028
version: 1
direction: direction/forward
top level: pSB2K3
start: 3242
end: 3393
orientation: inline

kanR
type: SequenceAnnotation
persistent identity: annotation1023020
display id: annotation1023020
version: 1
top level: pSB2K3
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/pSB2K3/annotation1023020/range1023020/1

https://synbiohub.org/public/igem/pSB2K3/annotation1023020/range1023020/1
type: Range
persistent identity: range1023020
display id: range1023020
version: 1
direction: direction/forward
top level: pSB2K3
start: 3433
end: 4248
orientation: inline

prefix
type: SequenceAnnotation
persistent identity: annotation1023021
display id: annotation1023021
version: 1
top level: pSB2K3
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/pSB2K3/annotation1023021/range1023021/1

https://synbiohub.org/public/igem/pSB2K3/annotation1023021/range1023021/1
type: Range
persistent identity: range1023021
display id: range1023021
version: 1
direction: direction/forward
top level: pSB2K3
start: 4404
end: 4425
orientation: inline

terminator stem loop
type: SequenceAnnotation
persistent identity: annotation1824014
display id: annotation1824014
version: 1
top level: pSB2K3
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/pSB2K3/annotation1824014/range1824014/1

https://synbiohub.org/public/igem/pSB2K3/annotation1824014/range1824014/1
type: Range
persistent identity: range1824014
display id: range1824014
version: 1
direction: direction/forward
top level: pSB2K3
start: 30
end: 64
orientation: inline

F1 Replication Origin
type: SequenceAnnotation
persistent identity: annotation1023023
display id: annotation1023023
version: 1
top level: pSB2K3
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/pSB2K3/annotation1023023/range1023023/1

https://synbiohub.org/public/igem/pSB2K3/annotation1023023/range1023023/1
type: Range
persistent identity: range1023023
display id: range1023023
version: 1
direction: direction/forward
top level: pSB2K3
start: 242
end: 290
orientation: inline

oriS Replication Origin (rev)
type: SequenceAnnotation
persistent identity: annotation1023025
display id: annotation1023025
version: 1
top level: pSB2K3
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/pSB2K3/annotation1023025/range1023025/1

https://synbiohub.org/public/igem/pSB2K3/annotation1023025/range1023025/1
type: Range
persistent identity: range1023025
display id: range1023025
version: 1
direction: direction/reverse
top level: pSB2K3
start: 1463
end: 1679
orientation: inline

suffix
type: SequenceAnnotation
persistent identity: annotation1023022
display id: annotation1023022
version: 1
top level: pSB2K3
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/pSB2K3/annotation1023022/range1023022/1

https://synbiohub.org/public/igem/pSB2K3/annotation1023022/range1023022/1
type: Range
persistent identity: range1023022
display id: range1023022
version: 1
direction: direction/forward
top level: pSB2K3
start: 1
end: 21
orientation: inline

VF2
type: SequenceAnnotation
persistent identity: annotation1782484
display id: annotation1782484
version: 1
top level: pSB2K3
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/pSB2K3/annotation1782484/range1782484/1

https://synbiohub.org/public/igem/pSB2K3/annotation1782484/range1782484/1
type: Range
persistent identity: range1782484
display id: range1782484
version: 1
direction: direction/forward
top level: pSB2K3
start: 4286
end: 4305
orientation: inline

deletion of two bases
type: SequenceAnnotation
persistent identity: annotation1823781
display id: annotation1823781
version: 1
top level: pSB2K3
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB2K3/annotation1823781/range1823781/1

https://synbiohub.org/public/igem/pSB2K3/annotation1823781/range1823781/1
type: Range
persistent identity: range1823781
display id: range1823781
version: 1
direction: direction/forward
top level: pSB2K3
start: 34
end: 35
orientation: inline

VR
type: SequenceAnnotation
persistent identity: annotation1782483
display id: annotation1782483
version: 1
top level: pSB2K3
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/pSB2K3/annotation1782483/range1782483/1

https://synbiohub.org/public/igem/pSB2K3/annotation1782483/range1782483/1
type: Range
persistent identity: range1782483
display id: range1782483
version: 1
direction: direction/reverse
top level: pSB2K3
start: 157
end: 176
orientation: inline