Cloning plasmid used by Geneart for iGEM parts. This plasmid does not have a standard BioBrick cloning site.
This plasmid needs to be checked to se where arets have actually been inserted. - Randy
The sequence of the plasmid that contains BBa_I712016.
The BioBrick prefix is missing the 3' G and the BioBrick suffix is missing the 5' T.
beta-lactamase ampicillin resistance marker
Cloning plasmid used by Geneart for iGEM parts. This plasmid does not have a standard BioBrick cloning site. Will be adding information once obtained from Justin Pahara (iGEM 07, Alberta). Vector is AmpR.
This plasmid needs to be checked to see where parts have actually been inserted. The sequence for this plasmid needs to be rotated so that the suffix and prefix are at the ends.
pUC19 with a BioBrick cloning site (Replaced by pSB1A3)
This plasmid is pUC19 with a BioBricks cloning site (EcoRI-NotI-XbaI-SpeI-NotI-PstI) located between the EcoRI and BamHI sites in the multiple cloning site of pUC19.
It is not entirely clear whether there are two contigous copies of EcoRI restriction site or one copy of the EcoRI in this vector. Sequence information did not include this section of the vector. In this part, only a single EcoRI restriction site is shown.
pUC19 is an extremely common cloning vector. This particular version is entered in the registry because several parts synthesized by Codon Devices (BBa_P1002, BBa_P1003, BBa_P1004, BBa_P1005, BBa_I50040) were returned in this vector. Sequence information can be found at http://www.fermentas.com/techinfo/nucleicacids/sequences/puc19.txt