//plasmidbackbone/operation
type: Collection
persistent identity: plasmidbackbone operation
display id: plasmidbackbone_operation
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
owned by: james
owned by: myers
member: pSBBs2E
member: BBa_K864013
member: BBa_J179000
member: pSB8K15
member: pSBBs4S
member: pSB4C1
member: pSB4A5
member: BBa_J64100
member: BAC
member: pSB2K4
member: BBa_K864006
member: pSB8A15FRT
member: BBa_K106672
member: BBa_K106693
member: BBa_J179002
member: BBa_K864015
member: pSB4K5
member: pSB4T5
member: pSB4C50
member: BBa_K864011
member: BBa_K106697
member: BBa_K864010
member: pSB3K1
member: BBa_K864003
member: pSac-Cm_BB
member: Transfecti
member: pSB8A15
member: pSB8S15FRT
member: BBa_K106698
member: K592200
member: pSB4C5
member: Eukaryotic
member: pSB8C15
member: BBa_K125000
member: BBa_K864004
member: Adh1P Acce
member: pSB8K15FRT
member: pSBBs1C
member: BBa_K864000
member: pSB8C15FRT
member: BBa_K864005
member: BBa_J179001
member: Cyc1P Acce
member: BBa_K864014
member: BBa_K864012
member: pSB3K3
member: pSB8S15
member: pSB4K50
member: pTG262-BB
member: pSB2K3
member: BBa_K864002
member: pSB3C5
member: BBa_K864007
member: BBa_K106670
member: pSac-Cm_B
member: pSB6A1
member: pSB4A50
member: BBa_K864008
member: pSB3K5
member: BBa_K864001
member: pSB3T5
member: pSB1AK8
member: BBa_K864009

BBa_K864009
type: ComponentDefinition
persistent identity: BBa K864009
display id: BBa_K864009
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Low copy BioBrick standard vector with LacI
creator: Erik Lundin
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:37Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1124_
m user id: 0
owner id: 9827
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: pSB4A15Iq is a BioBrick standard vector with low copy pSC101 replication origin (~5 copies per cell) and ampicillin antibiotic resistance marker, especially usable for Lambda Red recombineering in E coli. The backbone sequence is based on pSB3T5, but the E coli His operon terminator BBa_B0053 has been replaced with the late terminator of the Salmonella phage P22. This vector carries a LacIq cassette on the backbone for tight repression of any lac promoter cloned into the cloning site.
mutable notes: The resistance cassette is terminated by a Lux bidirectional terminator. Adjacent to this terminator there is a designed primer binding site to where the forward primer for lambda red can be designed to bind to no matter what the insert in the cloning site is or what the resistance is. The resistance cassette is flanked by SalI and SacI restriction sites for easy switching of resistance in the backbone.
mutable provenance: Based on BBa_K864001
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: PlacIq promoter
sequence annotation: T3 terminator
sequence annotation: P22 late terminator
sequence annotation: lacI
sequence annotation: pSC101 ori
sequence annotation: Promoter
sequence annotation: -10
sequence annotation: tonB terminator
sequence annotation: cmR
sequence annotation: rrnC
sequence annotation: -35
sequence annotation: Lux terminator
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_K864009_sequence/1

PlacIq promoter
type: SequenceAnnotation
persistent identity: annotation2202845
display id: annotation2202845
version: 1
top level: BBa_K864009
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K864009/annotation2202845/range2202845/1

https://synbiohub.org/public/igem/BBa_K864009/annotation2202845/range2202845/1
type: Range
persistent identity: range2202845
display id: range2202845
version: 1
direction: direction/forward
top level: BBa_K864009
start: 2439
end: 2494
orientation: inline

T3 terminator
type: SequenceAnnotation
persistent identity: annotation2202853
display id: annotation2202853
version: 1
top level: BBa_K864009
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864009/annotation2202853/range2202853/1

https://synbiohub.org/public/igem/BBa_K864009/annotation2202853/range2202853/1
type: Range
persistent identity: range2202853
display id: range2202853
version: 1
direction: direction/forward
top level: BBa_K864009
start: 4522
end: 4599
orientation: inline

P22 late terminator
type: SequenceAnnotation
persistent identity: annotation2202852
display id: annotation2202852
version: 1
top level: BBa_K864009
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K864009/annotation2202852/range2202852/1

https://synbiohub.org/public/igem/BBa_K864009/annotation2202852/range2202852/1
type: Range
persistent identity: range2202852
display id: range2202852
version: 1
direction: direction/forward
top level: BBa_K864009
start: 4461
end: 4501
orientation: inline

lacI
type: SequenceAnnotation
persistent identity: annotation2202846
display id: annotation2202846
version: 1
top level: BBa_K864009
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K864009/annotation2202846/range2202846/1

https://synbiohub.org/public/igem/BBa_K864009/annotation2202846/range2202846/1
type: Range
persistent identity: range2202846
display id: range2202846
version: 1
direction: direction/forward
top level: BBa_K864009
start: 2521
end: 3603
orientation: inline

pSC101 ori
type: SequenceAnnotation
persistent identity: annotation2202828
display id: annotation2202828
version: 1
top level: BBa_K864009
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864009/annotation2202828/range2202828/1

https://synbiohub.org/public/igem/BBa_K864009/annotation2202828/range2202828/1
type: Range
persistent identity: range2202828
display id: range2202828
version: 1
direction: direction/forward
top level: BBa_K864009
start: 190
end: 2405
orientation: inline

Promoter
type: SequenceAnnotation
persistent identity: annotation2202844
display id: annotation2202844
version: 1
top level: BBa_K864009
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K864009/annotation2202844/range2202844/1

https://synbiohub.org/public/igem/BBa_K864009/annotation2202844/range2202844/1
type: Range
persistent identity: range2202844
display id: range2202844
version: 1
direction: direction/reverse
top level: BBa_K864009
start: 4415
end: 4441
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2202857
display id: annotation2202857
version: 1
top level: BBa_K864009
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864009/annotation2202857/range2202857/1

https://synbiohub.org/public/igem/BBa_K864009/annotation2202857/range2202857/1
type: Range
persistent identity: range2202857
display id: range2202857
version: 1
direction: direction/forward
top level: BBa_K864009
start: 2464
end: 2469
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2202856
display id: annotation2202856
version: 1
top level: BBa_K864009
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864009/annotation2202856/range2202856/1

https://synbiohub.org/public/igem/BBa_K864009/annotation2202856/range2202856/1
type: Range
persistent identity: range2202856
display id: range2202856
version: 1
direction: direction/forward
top level: BBa_K864009
start: 903
end: 908
orientation: inline

tonB terminator
type: SequenceAnnotation
persistent identity: annotation2202854
display id: annotation2202854
version: 1
top level: BBa_K864009
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864009/annotation2202854/range2202854/1

https://synbiohub.org/public/igem/BBa_K864009/annotation2202854/range2202854/1
type: Range
persistent identity: range2202854
display id: range2202854
version: 1
direction: direction/forward
top level: BBa_K864009
start: 47
end: 115
orientation: inline

cmR
type: SequenceAnnotation
persistent identity: annotation2202847
display id: annotation2202847
version: 1
top level: BBa_K864009
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K864009/annotation2202847/range2202847/1

https://synbiohub.org/public/igem/BBa_K864009/annotation2202847/range2202847/1
type: Range
persistent identity: range2202847
display id: range2202847
version: 1
direction: direction/reverse
top level: BBa_K864009
start: 3686
end: 4348
orientation: inline

rrnC
type: SequenceAnnotation
persistent identity: annotation2202855
display id: annotation2202855
version: 1
top level: BBa_K864009
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864009/annotation2202855/range2202855/1

https://synbiohub.org/public/igem/BBa_K864009/annotation2202855/range2202855/1
type: Range
persistent identity: range2202855
display id: range2202855
version: 1
direction: direction/forward
top level: BBa_K864009
start: 136
end: 175
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2202849
display id: annotation2202849
version: 1
top level: BBa_K864009
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864009/annotation2202849/range2202849/1

https://synbiohub.org/public/igem/BBa_K864009/annotation2202849/range2202849/1
type: Range
persistent identity: range2202849
display id: range2202849
version: 1
direction: direction/forward
top level: BBa_K864009
start: 881
end: 886
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2202850
display id: annotation2202850
version: 1
top level: BBa_K864009
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864009/annotation2202850/range2202850/1

https://synbiohub.org/public/igem/BBa_K864009/annotation2202850/range2202850/1
type: Range
persistent identity: range2202850
display id: range2202850
version: 1
direction: direction/forward
top level: BBa_K864009
start: 2441
end: 2446
orientation: inline

Lux terminator
type: SequenceAnnotation
persistent identity: annotation2202851
display id: annotation2202851
version: 1
top level: BBa_K864009
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K864009/annotation2202851/range2202851/1

https://synbiohub.org/public/igem/BBa_K864009/annotation2202851/range2202851/1
type: Range
persistent identity: range2202851
display id: range2202851
version: 1
direction: direction/reverse
top level: BBa_K864009
start: 3634
end: 3679
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2202848
display id: annotation2202848
version: 1
top level: BBa_K864009
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K864009/annotation2202848/range2202848/1

https://synbiohub.org/public/igem/BBa_K864009/annotation2202848/range2202848/1
type: Range
persistent identity: range2202848
display id: range2202848
version: 1
direction: direction/forward
top level: BBa_K864009
start: 2509
end: 2514
orientation: inline

BBa_K864006
type: ComponentDefinition
persistent identity: BBa K864006
display id: BBa_K864006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Low copy BioBrick standard vector
creator: Erik Lundin
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:37Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1124_
m user id: 0
owner id: 9827
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: pSB4K15(FRT) is a BioBrick standard vector with low copy pSC101 replication origin (~5 copies per cell) and kanamycin antibiotic resistance marker, especially usable for Lambda Red recombineering in E coli. The backbone sequence is based on pSB3T5, but the E coli His operon terminator BBa_B0053 has been replaced with the late terminator of the Salmonella phage P22.
mutable notes: The resistance cassette is terminated by a Lux bidirectional terminator. Adjacent to this terminator there is a designed primer binding site to where the forward primer for lambda red can be designed to bind to no matter what the insert in the cloning site is or what the resistance is. The resistance cassette is flanked by SalI and SacI restriction sites for easy switching of resistance in the backbone. The pSC101 origin of replication is flaked by NheI and MluI restriction sites for easy switching of origin of replication.
mutable provenance: Based on pSB3T5
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence: https://synbiohub.org/public/igem/BBa_K864006_sequence/1

pSB3C5
type: ComponentDefinition
persistent identity: pSB3C5
display id: pSB3C5
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Low to medium copy BioBrick standard vector
creator: Reshma Shetty
created: 2007-04-10T11:00:00Z
modified: 2015-05-08T01:14:55Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _41_
m user id: 0
owner id: 126
owning group id: 70
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: pSB3C5 is a low to medium copy plasmid based with chloramphenicol resistance. It has a p15A-derived replication origin. pSB3C5 is derived from the BioBricks vector scaffold BBa_I51001.
mutable notes: None.
mutable provenance: BBa_I51001 BioBricks vector scaffold, p15A based origin and a version of the chloramphenicol resistance cassette that has been cleaned of restriction sites.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
component: BBa_P1004
component: BBa_B0042
component: BBa_G00000
component: BBa_I50032
component: BBa_B0043
component: BBa_G0003
component: BBa_B0055
component: BBa_B0054
component: BBa_G00102
component: BBa_B0062
component: BBa_G0004
component: BBa_G00001
component: BBa_G00100
component: BBa_B0053
component: BBa_G0002
component: BBa_B0044
sequence annotation: BBa_B0055
sequence annotation: BBa_B0053
sequence annotation: BBa_B0062
sequence annotation: BBa_G00001
sequence annotation: BBa_G00100
sequence annotation: BBa_I50032
sequence annotation: BBa_B0043
sequence annotation: BBa_B0044
sequence annotation: BBa_B0042
sequence annotation: BBa_B0054
sequence annotation: BBa_G00102
sequence annotation: BBa_G0003
sequence annotation: BBa_G0002
sequence annotation: BBa_P1004
sequence annotation: BBa_G00000
sequence annotation: BBa_G0004
sequence: https://synbiohub.org/public/igem/pSB3C5_sequence/1

BBa_P1004
type: Component
persistent identity: component1928335
display id: component1928335
version: 1
top level: pSB3C5
access: public
definition: https://synbiohub.org/public/igem/BBa_P1004/1

BBa_B0042
type: Component
persistent identity: component1928349
display id: component1928349
version: 1
top level: pSB3C5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0042/1

BBa_G00000
type: Component
persistent identity: component1928356
display id: component1928356
version: 1
top level: pSB3C5
access: public
definition: https://synbiohub.org/public/igem/BBa_G00000/1

BBa_I50032
type: Component
persistent identity: component1928332
display id: component1928332
version: 1
top level: pSB3C5
access: public
definition: https://synbiohub.org/public/igem/BBa_I50032/1

BBa_B0043
type: Component
persistent identity: component1928354
display id: component1928354
version: 1
top level: pSB3C5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0043/1

BBa_G0003
type: Component
persistent identity: component1928329
display id: component1928329
version: 1
top level: pSB3C5
access: public
definition: https://synbiohub.org/public/igem/BBa_G0003/1

BBa_B0042
type: Component
persistent identity: component1928322
display id: component1928322
version: 1
top level: pSB3C5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0042/1

BBa_B0055
type: Component
persistent identity: component1928342
display id: component1928342
version: 1
top level: pSB3C5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0055/1

BBa_B0054
type: Component
persistent identity: component1928325
display id: component1928325
version: 1
top level: pSB3C5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0054/1

BBa_G00102
type: Component
persistent identity: component1928326
display id: component1928326
version: 1
top level: pSB3C5
access: public
definition: https://synbiohub.org/public/igem/BBa_G00102/1

BBa_B0062
type: Component
persistent identity: component1928328
display id: component1928328
version: 1
top level: pSB3C5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0062/1

BBa_G0004
type: Component
persistent identity: component1928336
display id: component1928336
version: 1
top level: pSB3C5
access: public
definition: https://synbiohub.org/public/igem/BBa_G0004/1

BBa_G00001
type: Component
persistent identity: component1928310
display id: component1928310
version: 1
top level: pSB3C5
access: public
definition: https://synbiohub.org/public/igem/BBa_G00001/1

BBa_G00100
type: Component
persistent identity: component1928339
display id: component1928339
version: 1
top level: pSB3C5
access: public
definition: https://synbiohub.org/public/igem/BBa_G00100/1

BBa_B0053
type: Component
persistent identity: component1928338
display id: component1928338
version: 1
top level: pSB3C5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0053/1

BBa_G0002
type: Component
persistent identity: component1928333
display id: component1928333
version: 1
top level: pSB3C5
access: public
definition: https://synbiohub.org/public/igem/BBa_G0002/1

BBa_B0044
type: Component
persistent identity: component1928315
display id: component1928315
version: 1
top level: pSB3C5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0044/1

BBa_B0055
type: SequenceAnnotation
persistent identity: annotation1928342
display id: annotation1928342
version: 1
top level: pSB3C5
location: https://synbiohub.org/public/igem/pSB3C5/annotation1928342/range1928342/1
component: BBa_B0055

https://synbiohub.org/public/igem/pSB3C5/annotation1928342/range1928342/1
type: Range
persistent identity: range1928342
display id: range1928342
version: 1
direction: direction/reverse
top level: pSB3C5
start: 2614
end: 2691
orientation: inline

BBa_B0053
type: SequenceAnnotation
persistent identity: annotation1928338
display id: annotation1928338
version: 1
top level: pSB3C5
location: https://synbiohub.org/public/igem/pSB3C5/annotation1928338/range1928338/1
component: BBa_B0053

https://synbiohub.org/public/igem/pSB3C5/annotation1928338/range1928338/1
type: Range
persistent identity: range1928338
display id: range1928338
version: 1
direction: direction/reverse
top level: pSB3C5
start: 2522
end: 2593
orientation: inline

BBa_B0062
type: SequenceAnnotation
persistent identity: annotation1928328
display id: annotation1928328
version: 1
top level: pSB3C5
location: https://synbiohub.org/public/igem/pSB3C5/annotation1928328/range1928328/1
component: BBa_B0062

https://synbiohub.org/public/igem/pSB3C5/annotation1928328/range1928328/1
type: Range
persistent identity: range1928328
display id: range1928328
version: 1
direction: direction/reverse
top level: pSB3C5
start: 136
end: 176
orientation: inline

BBa_G00001
type: SequenceAnnotation
persistent identity: annotation1928310
display id: annotation1928310
version: 1
top level: pSB3C5
location: https://synbiohub.org/public/igem/pSB3C5/annotation1928310/range1928310/1
component: BBa_G00001

https://synbiohub.org/public/igem/pSB3C5/annotation1928310/range1928310/1
type: Range
persistent identity: range1928310
display id: range1928310
version: 1
direction: direction/reverse
top level: pSB3C5
start: 1
end: 21
orientation: inline

BBa_G00100
type: SequenceAnnotation
persistent identity: annotation1928339
display id: annotation1928339
version: 1
top level: pSB3C5
location: https://synbiohub.org/public/igem/pSB3C5/annotation1928339/range1928339/1
component: BBa_G00100

https://synbiohub.org/public/igem/pSB3C5/annotation1928339/range1928339/1
type: Range
persistent identity: range1928339
display id: range1928339
version: 1
direction: direction/reverse
top level: pSB3C5
start: 2594
end: 2613
orientation: inline

BBa_I50032
type: SequenceAnnotation
persistent identity: annotation1928332
display id: annotation1928332
version: 1
top level: pSB3C5
location: https://synbiohub.org/public/igem/pSB3C5/annotation1928332/range1928332/1
component: BBa_I50032

https://synbiohub.org/public/igem/pSB3C5/annotation1928332/range1928332/1
type: Range
persistent identity: range1928332
display id: range1928332
version: 1
direction: direction/reverse
top level: pSB3C5
start: 184
end: 1737
orientation: inline

BBa_B0043
type: SequenceAnnotation
persistent identity: annotation1928354
display id: annotation1928354
version: 1
top level: pSB3C5
location: https://synbiohub.org/public/igem/pSB3C5/annotation1928354/range1928354/1
component: BBa_B0043

https://synbiohub.org/public/igem/pSB3C5/annotation1928354/range1928354/1
type: Range
persistent identity: range1928354
display id: range1928354
version: 1
direction: direction/reverse
top level: pSB3C5
start: 2704
end: 2716
orientation: inline

BBa_B0044
type: SequenceAnnotation
persistent identity: annotation1928315
display id: annotation1928315
version: 1
top level: pSB3C5
location: https://synbiohub.org/public/igem/pSB3C5/annotation1928315/range1928315/1
component: BBa_B0044

https://synbiohub.org/public/igem/pSB3C5/annotation1928315/range1928315/1
type: Range
persistent identity: range1928315
display id: range1928315
version: 1
direction: direction/reverse
top level: pSB3C5
start: 22
end: 34
orientation: inline

BBa_B0042
type: SequenceAnnotation
persistent identity: annotation1928349
display id: annotation1928349
version: 1
top level: pSB3C5
location: https://synbiohub.org/public/igem/pSB3C5/annotation1928349/range1928349/1
component: BBa_B0042

https://synbiohub.org/public/igem/pSB3C5/annotation1928349/range1928349/1
type: Range
persistent identity: range1928349
display id: range1928349
version: 1
direction: direction/reverse
top level: pSB3C5
start: 2692
end: 2703
orientation: inline

BBa_B0054
type: SequenceAnnotation
persistent identity: annotation1928325
display id: annotation1928325
version: 1
top level: pSB3C5
location: https://synbiohub.org/public/igem/pSB3C5/annotation1928325/range1928325/1
component: BBa_B0054

https://synbiohub.org/public/igem/pSB3C5/annotation1928325/range1928325/1
type: Range
persistent identity: range1928325
display id: range1928325
version: 1
direction: direction/reverse
top level: pSB3C5
start: 47
end: 115
orientation: inline

BBa_G00102
type: SequenceAnnotation
persistent identity: annotation1928326
display id: annotation1928326
version: 1
top level: pSB3C5
location: https://synbiohub.org/public/igem/pSB3C5/annotation1928326/range1928326/1
component: BBa_G00102

https://synbiohub.org/public/igem/pSB3C5/annotation1928326/range1928326/1
type: Range
persistent identity: range1928326
display id: range1928326
version: 1
direction: direction/reverse
top level: pSB3C5
start: 116
end: 135
orientation: inline

BBa_G0003
type: SequenceAnnotation
persistent identity: annotation1928329
display id: annotation1928329
version: 1
top level: pSB3C5
location: https://synbiohub.org/public/igem/pSB3C5/annotation1928329/range1928329/1
component: BBa_G0003

https://synbiohub.org/public/igem/pSB3C5/annotation1928329/range1928329/1
type: Range
persistent identity: range1928329
display id: range1928329
version: 1
direction: direction/reverse
top level: pSB3C5
start: 177
end: 183
orientation: inline

BBa_G0002
type: SequenceAnnotation
persistent identity: annotation1928333
display id: annotation1928333
version: 1
top level: pSB3C5
location: https://synbiohub.org/public/igem/pSB3C5/annotation1928333/range1928333/1
component: BBa_G0002

https://synbiohub.org/public/igem/pSB3C5/annotation1928333/range1928333/1
type: Range
persistent identity: range1928333
display id: range1928333
version: 1
direction: direction/reverse
top level: pSB3C5
start: 1738
end: 1745
orientation: inline

BBa_B0042
type: SequenceAnnotation
persistent identity: annotation1928322
display id: annotation1928322
version: 1
top level: pSB3C5
location: https://synbiohub.org/public/igem/pSB3C5/annotation1928322/range1928322/1
component: BBa_B0042

https://synbiohub.org/public/igem/pSB3C5/annotation1928322/range1928322/1
type: Range
persistent identity: range1928322
display id: range1928322
version: 1
direction: direction/reverse
top level: pSB3C5
start: 35
end: 46
orientation: inline

BBa_P1004
type: SequenceAnnotation
persistent identity: annotation1928335
display id: annotation1928335
version: 1
top level: pSB3C5
location: https://synbiohub.org/public/igem/pSB3C5/annotation1928335/range1928335/1
component: BBa_P1004

https://synbiohub.org/public/igem/pSB3C5/annotation1928335/range1928335/1
type: Range
persistent identity: range1928335
display id: range1928335
version: 1
direction: direction/reverse
top level: pSB3C5
start: 1746
end: 2514
orientation: inline

BBa_G00000
type: SequenceAnnotation
persistent identity: annotation1928356
display id: annotation1928356
version: 1
top level: pSB3C5
location: https://synbiohub.org/public/igem/pSB3C5/annotation1928356/range1928356/1
component: BBa_G00000

https://synbiohub.org/public/igem/pSB3C5/annotation1928356/range1928356/1
type: Range
persistent identity: range1928356
display id: range1928356
version: 1
direction: direction/reverse
top level: pSB3C5
start: 2717
end: 2738
orientation: inline

BBa_G0004
type: SequenceAnnotation
persistent identity: annotation1928336
display id: annotation1928336
version: 1
top level: pSB3C5
location: https://synbiohub.org/public/igem/pSB3C5/annotation1928336/range1928336/1
component: BBa_G0004

https://synbiohub.org/public/igem/pSB3C5/annotation1928336/range1928336/1
type: Range
persistent identity: range1928336
display id: range1928336
version: 1
direction: direction/reverse
top level: pSB3C5
start: 2515
end: 2521
orientation: inline

BBa_K106693
type: ComponentDefinition
persistent identity: BBa K106693
display id: BBa_K106693
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: AarI A!D acceptor vector (pRS315, Cyc1P, Adh1t)
creator: Andrew Horwitz
created: 2008-10-28T12:00:00Z
modified: 2015-05-08T01:09:00Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _226_
m user id: 0
owner id: 2659
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: This is a special variant acceptor vector for AarI cloning. The "A" site used here, termed "A!" is an alternate end used when no amino-terminal fusions are tolerated. Please see the UCSF 2008 iGEM wiki for more information on AarI cloning. This was designed specifically for use with Sir3 and amino-terminal domains of Sir3.
mutable notes: Note that this vector will NOT accept standard AarI "A" parts.
mutable provenance: Modified pRS315 plasmid.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: ampicillin resistance marker
sequence annotation: Adh1 terminator
sequence annotation: ampicillin resistance promoter
sequence annotation: pBR322 origin
sequence annotation: lacZalpha reporter
sequence annotation: Start codon
sequence annotation: AarI A!D MCS
sequence annotation: T3 promoter
sequence annotation: Adh1 promoter
sequence annotation: Adh1P! promoter
sequence annotation: LEU2 marker
sequence annotation: lac promoter
sequence annotation: A overhang
sequence annotation: T7 promoter
sequence annotation: f1 origin
sequence annotation: D overhang
sequence annotation: Stop codons in all 3 frames
sequence annotation: CEN ARS
sequence: https://synbiohub.org/public/igem/BBa_K106693_sequence/1

ampicillin resistance marker
type: SequenceAnnotation
persistent identity: annotation1994690
display id: annotation1994690
version: 1
top level: BBa_K106693
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K106693/annotation1994690/range1994690/1

https://synbiohub.org/public/igem/BBa_K106693/annotation1994690/range1994690/1
type: Range
persistent identity: range1994690
display id: range1994690
version: 1
direction: direction/forward
top level: BBa_K106693
start: 4053
end: 4911
orientation: inline

Adh1 terminator
type: SequenceAnnotation
persistent identity: annotation1994693
display id: annotation1994693
version: 1
top level: BBa_K106693
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K106693/annotation1994693/range1994693/1

https://synbiohub.org/public/igem/BBa_K106693/annotation1994693/range1994693/1
type: Range
persistent identity: range1994693
display id: range1994693
version: 1
direction: direction/forward
top level: BBa_K106693
start: 18
end: 211
orientation: inline

ampicillin resistance promoter
type: SequenceAnnotation
persistent identity: annotation1999719
display id: annotation1999719
version: 1
top level: BBa_K106693
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K106693/annotation1999719/range1999719/1

https://synbiohub.org/public/igem/BBa_K106693/annotation1999719/range1999719/1
type: Range
persistent identity: range1999719
display id: range1999719
version: 1
direction: direction/forward
top level: BBa_K106693
start: 3983
end: 4011
orientation: inline

pBR322 origin
type: SequenceAnnotation
persistent identity: annotation1999720
display id: annotation1999720
version: 1
top level: BBa_K106693
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K106693/annotation1999720/range1999720/1

https://synbiohub.org/public/igem/BBa_K106693/annotation1999720/range1999720/1
type: Range
persistent identity: range1999720
display id: range1999720
version: 1
direction: direction/forward
top level: BBa_K106693
start: 5068
end: 5687
orientation: inline

lacZalpha reporter
type: SequenceAnnotation
persistent identity: annotation1999717
display id: annotation1999717
version: 1
top level: BBa_K106693
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K106693/annotation1999717/range1999717/1

https://synbiohub.org/public/igem/BBa_K106693/annotation1999717/range1999717/1
type: Range
persistent identity: range1999717
display id: range1999717
version: 1
direction: direction/forward
top level: BBa_K106693
start: 246
end: 405
orientation: inline

Start codon
type: SequenceAnnotation
persistent identity: annotation1999726
display id: annotation1999726
version: 1
top level: BBa_K106693
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K106693/annotation1999726/range1999726/1

https://synbiohub.org/public/igem/BBa_K106693/annotation1999726/range1999726/1
type: Range
persistent identity: range1999726
display id: range1999726
version: 1
direction: direction/forward
top level: BBa_K106693
start: 7638
end: 7640
orientation: inline

AarI A!D MCS
type: SequenceAnnotation
persistent identity: annotation1994692
display id: annotation1994692
version: 1
top level: BBa_K106693
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K106693/annotation1994692/range1994692/1

https://synbiohub.org/public/igem/BBa_K106693/annotation1994692/range1994692/1
type: Range
persistent identity: range1994692
display id: range1994692
version: 1
direction: direction/forward
top level: BBa_K106693
start: 4305
end: 4381
orientation: inline

T3 promoter
type: SequenceAnnotation
persistent identity: annotation1999722
display id: annotation1999722
version: 1
top level: BBa_K106693
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K106693/annotation1999722/range1999722/1

https://synbiohub.org/public/igem/BBa_K106693/annotation1999722/range1999722/1
type: Range
persistent identity: range1999722
display id: range1999722
version: 1
direction: direction/forward
top level: BBa_K106693
start: 6095
end: 6114
orientation: inline

Adh1 promoter
type: SequenceAnnotation
persistent identity: annotation1999723
display id: annotation1999723
version: 1
top level: BBa_K106693
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K106693/annotation1999723/range1999723/1

https://synbiohub.org/public/igem/BBa_K106693/annotation1999723/range1999723/1
type: Range
persistent identity: range1999723
display id: range1999723
version: 1
direction: direction/forward
top level: BBa_K106693
start: 7229
end: 7625
orientation: inline

Adh1P! promoter
type: SequenceAnnotation
persistent identity: annotation1994691
display id: annotation1994691
version: 1
top level: BBa_K106693
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K106693/annotation1994691/range1994691/1

https://synbiohub.org/public/igem/BBa_K106693/annotation1994691/range1994691/1
type: Range
persistent identity: range1994691
display id: range1994691
version: 1
direction: direction/forward
top level: BBa_K106693
start: 6132
end: 7642
orientation: inline

LEU2 marker
type: SequenceAnnotation
persistent identity: annotation1994688
display id: annotation1994688
version: 1
top level: BBa_K106693
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K106693/annotation1994688/range1994688/1

https://synbiohub.org/public/igem/BBa_K106693/annotation1994688/range1994688/1
type: Range
persistent identity: range1994688
display id: range1994688
version: 1
direction: direction/forward
top level: BBa_K106693
start: 1570
end: 2676
orientation: inline

lac promoter
type: SequenceAnnotation
persistent identity: annotation1999721
display id: annotation1999721
version: 1
top level: BBa_K106693
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K106693/annotation1999721/range1999721/1

https://synbiohub.org/public/igem/BBa_K106693/annotation1999721/range1999721/1
type: Range
persistent identity: range1999721
display id: range1999721
version: 1
direction: direction/forward
top level: BBa_K106693
start: 5996
end: 6025
orientation: inline

A overhang
type: SequenceAnnotation
persistent identity: annotation1999725
display id: annotation1999725
version: 1
top level: BBa_K106693
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K106693/annotation1999725/range1999725/1

https://synbiohub.org/public/igem/BBa_K106693/annotation1999725/range1999725/1
type: Range
persistent identity: range1999725
display id: range1999725
version: 1
direction: direction/forward
top level: BBa_K106693
start: 7647
end: 7650
orientation: inline

T7 promoter
type: SequenceAnnotation
persistent identity: annotation1999716
display id: annotation1999716
version: 1
top level: BBa_K106693
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K106693/annotation1999716/range1999716/1

https://synbiohub.org/public/igem/BBa_K106693/annotation1999716/range1999716/1
type: Range
persistent identity: range1999716
display id: range1999716
version: 1
direction: direction/forward
top level: BBa_K106693
start: 224
end: 242
orientation: inline

f1 origin
type: SequenceAnnotation
persistent identity: annotation1999718
display id: annotation1999718
version: 1
top level: BBa_K106693
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K106693/annotation1999718/range1999718/1

https://synbiohub.org/public/igem/BBa_K106693/annotation1999718/range1999718/1
type: Range
persistent identity: range1999718
display id: range1999718
version: 1
direction: direction/forward
top level: BBa_K106693
start: 427
end: 733
orientation: inline

D overhang
type: SequenceAnnotation
persistent identity: annotation1999724
display id: annotation1999724
version: 1
top level: BBa_K106693
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K106693/annotation1999724/range1999724/1

https://synbiohub.org/public/igem/BBa_K106693/annotation1999724/range1999724/1
type: Range
persistent identity: range1999724
display id: range1999724
version: 1
direction: direction/forward
top level: BBa_K106693
start: 1
end: 4
orientation: inline

Stop codons in all 3 frames
type: SequenceAnnotation
persistent identity: annotation1999727
display id: annotation1999727
version: 1
top level: BBa_K106693
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K106693/annotation1999727/range1999727/1

https://synbiohub.org/public/igem/BBa_K106693/annotation1999727/range1999727/1
type: Range
persistent identity: range1999727
display id: range1999727
version: 1
direction: direction/forward
top level: BBa_K106693
start: 11
end: 21
orientation: inline

CEN ARS
type: SequenceAnnotation
persistent identity: annotation1994689
display id: annotation1994689
version: 1
top level: BBa_K106693
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K106693/annotation1994689/range1994689/1

https://synbiohub.org/public/igem/BBa_K106693/annotation1994689/range1994689/1
type: Range
persistent identity: range1994689
display id: range1994689
version: 1
direction: direction/reverse
top level: BBa_K106693
start: 3403
end: 3921
orientation: inline

pSB4K5
type: ComponentDefinition
persistent identity: pSB4K5
display id: pSB4K5
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Low copy BioBrick standard vector
creator: Reshma Shetty
created: 2007-02-28T12:00:00Z
modified: 2015-05-08T01:14:55Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _41_
m user id: 0
owner id: 126
owning group id: 70
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: pSB4K5 is a low copy plasmid with kanamycin resistance. It has a repA pSC101-derived replication origin. (Copy number ~10-12, Lutz and Bujard, 1997.) It is derived from the BioBricks vector scaffold BBa_I51001.
mutable notes: See http://openwetware.org/wiki/Synthetic_Biology:Vectors/pSB%2A%2A5_design for more information.
mutable provenance: BBa_I51001 vector scaffold, BBa_I50040 replication origin and BBa_P1003 antibiotic resistance marker.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
component: BBa_I50041
component: BBa_B0044
component: BBa_B0042
component: BBa_P1007
component: BBa_G00000
component: BBa_G00102
component: BBa_B0043
component: BBa_B0053
component: BBa_G00100
component: BBa_G0001
component: BBa_B0062
component: BBa_G0006
component: BBa_B0054
component: BBa_B0055
component: BBa_G0005
component: BBa_G00001
sequence annotation: BBa_G00000
sequence annotation: BBa_B0042
sequence annotation: BBa_G0005
sequence annotation: BBa_B0053
sequence annotation: BBa_G0006
sequence annotation: BBa_B0055
sequence annotation: BBa_B0054
sequence annotation: BBa_G00001
sequence annotation: BBa_I50041
sequence annotation: BBa_G0001
sequence annotation: BBa_G00102
sequence annotation: BBa_G00100
sequence annotation: BBa_B0044
sequence annotation: BBa_P1007
sequence annotation: BBa_B0043
sequence annotation: BBa_B0062
sequence: https://synbiohub.org/public/igem/pSB4K5_sequence/1

BBa_I50041
type: Component
persistent identity: component2236030
display id: component2236030
version: 1
top level: pSB4K5
access: public
definition: https://synbiohub.org/public/igem/BBa_I50041/1

BBa_B0044
type: Component
persistent identity: component2235984
display id: component2235984
version: 1
top level: pSB4K5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0044/1

BBa_B0042
type: Component
persistent identity: component2235991
display id: component2235991
version: 1
top level: pSB4K5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0042/1

BBa_P1007
type: Component
persistent identity: component2236003
display id: component2236003
version: 1
top level: pSB4K5
access: public
definition: https://synbiohub.org/public/igem/BBa_P1007/1

BBa_G00000
type: Component
persistent identity: component2236055
display id: component2236055
version: 1
top level: pSB4K5
access: public
definition: https://synbiohub.org/public/igem/BBa_G00000/1

BBa_G00102
type: Component
persistent identity: component2235996
display id: component2235996
version: 1
top level: pSB4K5
access: public
definition: https://synbiohub.org/public/igem/BBa_G00102/1

BBa_B0042
type: Component
persistent identity: component2236045
display id: component2236045
version: 1
top level: pSB4K5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0042/1

BBa_B0043
type: Component
persistent identity: component2236050
display id: component2236050
version: 1
top level: pSB4K5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0043/1

BBa_B0053
type: Component
persistent identity: component2236033
display id: component2236033
version: 1
top level: pSB4K5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0053/1

BBa_G00100
type: Component
persistent identity: component2236035
display id: component2236035
version: 1
top level: pSB4K5
access: public
definition: https://synbiohub.org/public/igem/BBa_G00100/1

BBa_G0001
type: Component
persistent identity: component2236004
display id: component2236004
version: 1
top level: pSB4K5
access: public
definition: https://synbiohub.org/public/igem/BBa_G0001/1

BBa_B0062
type: Component
persistent identity: component2235998
display id: component2235998
version: 1
top level: pSB4K5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0062/1

BBa_G0006
type: Component
persistent identity: component2236031
display id: component2236031
version: 1
top level: pSB4K5
access: public
definition: https://synbiohub.org/public/igem/BBa_G0006/1

BBa_B0054
type: Component
persistent identity: component2235994
display id: component2235994
version: 1
top level: pSB4K5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0054/1

BBa_B0055
type: Component
persistent identity: component2236038
display id: component2236038
version: 1
top level: pSB4K5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0055/1

BBa_G0005
type: Component
persistent identity: component2235999
display id: component2235999
version: 1
top level: pSB4K5
access: public
definition: https://synbiohub.org/public/igem/BBa_G0005/1

BBa_G00001
type: Component
persistent identity: component2235979
display id: component2235979
version: 1
top level: pSB4K5
access: public
definition: https://synbiohub.org/public/igem/BBa_G00001/1

BBa_G00000
type: SequenceAnnotation
persistent identity: annotation2236055
display id: annotation2236055
version: 1
top level: pSB4K5
location: https://synbiohub.org/public/igem/pSB4K5/annotation2236055/range2236055/1
component: BBa_G00000

https://synbiohub.org/public/igem/pSB4K5/annotation2236055/range2236055/1
type: Range
persistent identity: range2236055
display id: range2236055
version: 1
direction: direction/reverse
top level: pSB4K5
start: 3398
end: 3419
orientation: inline

BBa_B0042
type: SequenceAnnotation
persistent identity: annotation2236045
display id: annotation2236045
version: 1
top level: pSB4K5
location: https://synbiohub.org/public/igem/pSB4K5/annotation2236045/range2236045/1
component: BBa_B0042

https://synbiohub.org/public/igem/pSB4K5/annotation2236045/range2236045/1
type: Range
persistent identity: range2236045
display id: range2236045
version: 1
direction: direction/reverse
top level: pSB4K5
start: 3373
end: 3384
orientation: inline

BBa_G0005
type: SequenceAnnotation
persistent identity: annotation2235999
display id: annotation2235999
version: 1
top level: pSB4K5
location: https://synbiohub.org/public/igem/pSB4K5/annotation2235999/range2235999/1
component: BBa_G0005

https://synbiohub.org/public/igem/pSB4K5/annotation2235999/range2235999/1
type: Range
persistent identity: range2235999
display id: range2235999
version: 1
direction: direction/reverse
top level: pSB4K5
start: 177
end: 183
orientation: inline

BBa_B0053
type: SequenceAnnotation
persistent identity: annotation2236033
display id: annotation2236033
version: 1
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component: BBa_B0053

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display id: range2236033
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BBa_G0006
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display id: annotation2236031
version: 1
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component: BBa_G0006

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BBa_B0055
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component: BBa_B0055

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direction: direction/reverse
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BBa_B0054
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display id: annotation2235994
version: 1
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location: https://synbiohub.org/public/igem/pSB4K5/annotation2235994/range2235994/1
component: BBa_B0054

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type: Range
persistent identity: range2235994
display id: range2235994
version: 1
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orientation: inline

BBa_G00001
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display id: annotation2235979
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component: BBa_G00001

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display id: range2235979
version: 1
direction: direction/reverse
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orientation: inline

BBa_I50041
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display id: annotation2236030
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location: https://synbiohub.org/public/igem/pSB4K5/annotation2236030/range2236030/1
component: BBa_I50041

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display id: range2236030
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BBa_B0042
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display id: annotation2235991
version: 1
top level: pSB4K5
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component: BBa_B0042

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persistent identity: range2235991
display id: range2235991
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BBa_G0001
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display id: annotation2236004
version: 1
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component: BBa_G0001

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display id: range2236004
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BBa_G00102
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BBa_G00100
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component: BBa_G00100

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display id: range2236035
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BBa_B0044
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component: BBa_B0044

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BBa_P1007
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component: BBa_P1007

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type: Range
persistent identity: range2236003
display id: range2236003
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end: 1150
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BBa_B0043
type: SequenceAnnotation
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display id: annotation2236050
version: 1
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component: BBa_B0043

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display id: range2236050
version: 1
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BBa_B0062
type: SequenceAnnotation
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display id: annotation2235998
version: 1
top level: pSB4K5
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component: BBa_B0062

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type: Range
persistent identity: range2235998
display id: range2235998
version: 1
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orientation: inline

pSB4K50
type: ComponentDefinition
persistent identity: BBa K1362097
display id: BBa_K1362097
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Low copy BioBrick expression backbone carrying Kan resistance
creator: Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena Büscher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Schäfer, Carolin Schmelas, Silvan Schmitz, Max Waldha
created: 2014-10-07T11:00:00Z
modified: 2015-05-08T01:10:04Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1738_
m user id: 0
owner id: 22830
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
component: BBa_B0055
component: BBa_B0053
component: BBa_B0062
component: BBa_G0005
component: BBa_G0001
component: BBa_G0006
component: BBa_G00100
component: BBa_G00001
component: BBa_I50041
component: BBa_K1362463
component: BBa_B0044
component: BBa_B0042
component: BBa_B0043
component: BBa_B0054
component: BBa_P1007
component: BBa_G00102
component: BBa_G00000
sequence annotation: BBa_G00001
sequence annotation: BBa_G0001
sequence annotation: BBa_P1007
sequence annotation: BBa_G00100
sequence annotation: BBa_B0055
sequence annotation: BBa_I50041
sequence annotation: BBa_B0053
sequence annotation: BBa_G00102
sequence annotation: BBa_B0062
sequence annotation: BBa_B0044
sequence annotation: BBa_B0042
sequence annotation: BBa_B0054
sequence annotation: BBa_G0006
sequence annotation: BBa_G0005
sequence annotation: BBa_B0043
sequence annotation: BBa_K1362463
sequence annotation: BBa_G00000
sequence: https://synbiohub.org/public/igem/BBa_K1362097_sequence/1

BBa_B0055
type: Component
persistent identity: component2409996
display id: component2409996
version: 1
top level: pSB4K50
access: public
definition: https://synbiohub.org/public/igem/BBa_B0055/1

BBa_B0053
type: Component
persistent identity: component2409991
display id: component2409991
version: 1
top level: pSB4K50
access: public
definition: https://synbiohub.org/public/igem/BBa_B0053/1

BBa_B0062
type: Component
persistent identity: component2409956
display id: component2409956
version: 1
top level: pSB4K50
access: public
definition: https://synbiohub.org/public/igem/BBa_B0062/1

BBa_G0005
type: Component
persistent identity: component2409957
display id: component2409957
version: 1
top level: pSB4K50
access: public
definition: https://synbiohub.org/public/igem/BBa_G0005/1

BBa_G0001
type: Component
persistent identity: component2409962
display id: component2409962
version: 1
top level: pSB4K50
access: public
definition: https://synbiohub.org/public/igem/BBa_G0001/1

BBa_G0006
type: Component
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display id: component2409989
version: 1
top level: pSB4K50
access: public
definition: https://synbiohub.org/public/igem/BBa_G0006/1

BBa_G00100
type: Component
persistent identity: component2409993
display id: component2409993
version: 1
top level: pSB4K50
access: public
definition: https://synbiohub.org/public/igem/BBa_G00100/1

BBa_G00001
type: Component
persistent identity: component2409937
display id: component2409937
version: 1
top level: pSB4K50
access: public
definition: https://synbiohub.org/public/igem/BBa_G00001/1

BBa_I50041
type: Component
persistent identity: component2409988
display id: component2409988
version: 1
top level: pSB4K50
access: public
definition: https://synbiohub.org/public/igem/BBa_I50041/1

BBa_K1362463
type: Component
persistent identity: component2410012
display id: component2410012
version: 1
top level: pSB4K50
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362463/1

BBa_B0044
type: Component
persistent identity: component2409942
display id: component2409942
version: 1
top level: pSB4K50
access: public
definition: https://synbiohub.org/public/igem/BBa_B0044/1

BBa_B0042
type: Component
persistent identity: component2410003
display id: component2410003
version: 1
top level: pSB4K50
access: public
definition: https://synbiohub.org/public/igem/BBa_B0042/1

BBa_B0043
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persistent identity: component2410008
display id: component2410008
version: 1
top level: pSB4K50
access: public
definition: https://synbiohub.org/public/igem/BBa_B0043/1

BBa_B0054
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version: 1
top level: pSB4K50
access: public
definition: https://synbiohub.org/public/igem/BBa_B0054/1

BBa_B0042
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persistent identity: component2409949
display id: component2409949
version: 1
top level: pSB4K50
access: public
definition: https://synbiohub.org/public/igem/BBa_B0042/1

BBa_P1007
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persistent identity: component2409961
display id: component2409961
version: 1
top level: pSB4K50
access: public
definition: https://synbiohub.org/public/igem/BBa_P1007/1

BBa_G00102
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persistent identity: component2409954
display id: component2409954
version: 1
top level: pSB4K50
access: public
definition: https://synbiohub.org/public/igem/BBa_G00102/1

BBa_G00000
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persistent identity: component2410017
display id: component2410017
version: 1
top level: pSB4K50
access: public
definition: https://synbiohub.org/public/igem/BBa_G00000/1

BBa_G00001
type: SequenceAnnotation
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display id: annotation2409937
version: 1
top level: pSB4K50
location: https://synbiohub.org/public/igem/BBa_K1362097/annotation2409937/range2409937/1
component: BBa_G00001

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version: 1
direction: direction/reverse
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orientation: inline

BBa_G0001
type: SequenceAnnotation
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display id: annotation2409962
version: 1
top level: pSB4K50
location: https://synbiohub.org/public/igem/BBa_K1362097/annotation2409962/range2409962/1
component: BBa_G0001

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direction: direction/reverse
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BBa_P1007
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display id: annotation2409961
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location: https://synbiohub.org/public/igem/BBa_K1362097/annotation2409961/range2409961/1
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persistent identity: range2409961
display id: range2409961
version: 1
direction: direction/reverse
top level: pSB4K50
start: 184
end: 1150
orientation: inline

BBa_G00100
type: SequenceAnnotation
persistent identity: annotation2409993
display id: annotation2409993
version: 1
top level: pSB4K50
location: https://synbiohub.org/public/igem/BBa_K1362097/annotation2409993/range2409993/1
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persistent identity: range2409993
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version: 1
direction: direction/reverse
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orientation: inline

BBa_B0055
type: SequenceAnnotation
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display id: annotation2409996
version: 1
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persistent identity: range2409996
display id: range2409996
version: 1
direction: direction/reverse
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start: 3295
end: 3372
orientation: inline

BBa_I50041
type: SequenceAnnotation
persistent identity: annotation2409988
display id: annotation2409988
version: 1
top level: pSB4K50
location: https://synbiohub.org/public/igem/BBa_K1362097/annotation2409988/range2409988/1
component: BBa_I50041

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type: Range
persistent identity: range2409988
display id: range2409988
version: 1
direction: direction/reverse
top level: pSB4K50
start: 1159
end: 3195
orientation: inline

BBa_B0053
type: SequenceAnnotation
persistent identity: annotation2409991
display id: annotation2409991
version: 1
top level: pSB4K50
location: https://synbiohub.org/public/igem/BBa_K1362097/annotation2409991/range2409991/1
component: BBa_B0053

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type: Range
persistent identity: range2409991
display id: range2409991
version: 1
direction: direction/reverse
top level: pSB4K50
start: 3203
end: 3274
orientation: inline

BBa_G00102
type: SequenceAnnotation
persistent identity: annotation2409954
display id: annotation2409954
version: 1
top level: pSB4K50
location: https://synbiohub.org/public/igem/BBa_K1362097/annotation2409954/range2409954/1
component: BBa_G00102

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type: Range
persistent identity: range2409954
display id: range2409954
version: 1
direction: direction/reverse
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end: 135
orientation: inline

BBa_B0062
type: SequenceAnnotation
persistent identity: annotation2409956
display id: annotation2409956
version: 1
top level: pSB4K50
location: https://synbiohub.org/public/igem/BBa_K1362097/annotation2409956/range2409956/1
component: BBa_B0062

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type: Range
persistent identity: range2409956
display id: range2409956
version: 1
direction: direction/reverse
top level: pSB4K50
start: 136
end: 176
orientation: inline

BBa_B0044
type: SequenceAnnotation
persistent identity: annotation2409942
display id: annotation2409942
version: 1
top level: pSB4K50
location: https://synbiohub.org/public/igem/BBa_K1362097/annotation2409942/range2409942/1
component: BBa_B0044

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type: Range
persistent identity: range2409942
display id: range2409942
version: 1
direction: direction/reverse
top level: pSB4K50
start: 22
end: 34
orientation: inline

BBa_B0042
type: SequenceAnnotation
persistent identity: annotation2409949
display id: annotation2409949
version: 1
top level: pSB4K50
location: https://synbiohub.org/public/igem/BBa_K1362097/annotation2409949/range2409949/1
component: BBa_B0042

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type: Range
persistent identity: range2409949
display id: range2409949
version: 1
direction: direction/reverse
top level: pSB4K50
start: 35
end: 46
orientation: inline

BBa_B0054
type: SequenceAnnotation
persistent identity: annotation2409952
display id: annotation2409952
version: 1
top level: pSB4K50
location: https://synbiohub.org/public/igem/BBa_K1362097/annotation2409952/range2409952/1
component: BBa_B0054

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type: Range
persistent identity: range2409952
display id: range2409952
version: 1
direction: direction/reverse
top level: pSB4K50
start: 47
end: 115
orientation: inline

BBa_G0006
type: SequenceAnnotation
persistent identity: annotation2409989
display id: annotation2409989
version: 1
top level: pSB4K50
location: https://synbiohub.org/public/igem/BBa_K1362097/annotation2409989/range2409989/1
component: BBa_G0006

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type: Range
persistent identity: range2409989
display id: range2409989
version: 1
direction: direction/reverse
top level: pSB4K50
start: 3196
end: 3202
orientation: inline

BBa_G0005
type: SequenceAnnotation
persistent identity: annotation2409957
display id: annotation2409957
version: 1
top level: pSB4K50
location: https://synbiohub.org/public/igem/BBa_K1362097/annotation2409957/range2409957/1
component: BBa_G0005

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type: Range
persistent identity: range2409957
display id: range2409957
version: 1
direction: direction/reverse
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start: 177
end: 183
orientation: inline

BBa_B0043
type: SequenceAnnotation
persistent identity: annotation2410008
display id: annotation2410008
version: 1
top level: pSB4K50
location: https://synbiohub.org/public/igem/BBa_K1362097/annotation2410008/range2410008/1
component: BBa_B0043

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type: Range
persistent identity: range2410008
display id: range2410008
version: 1
direction: direction/reverse
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start: 3385
end: 3397
orientation: inline

BBa_K1362463
type: SequenceAnnotation
persistent identity: annotation2410012
display id: annotation2410012
version: 1
top level: pSB4K50
location: https://synbiohub.org/public/igem/BBa_K1362097/annotation2410012/range2410012/1
component: BBa_K1362463

https://synbiohub.org/public/igem/BBa_K1362097/annotation2410012/range2410012/1
type: Range
persistent identity: range2410012
display id: range2410012
version: 1
direction: direction/reverse
top level: pSB4K50
start: 3398
end: 3448
orientation: inline

BBa_B0042
type: SequenceAnnotation
persistent identity: annotation2410003
display id: annotation2410003
version: 1
top level: pSB4K50
location: https://synbiohub.org/public/igem/BBa_K1362097/annotation2410003/range2410003/1
component: BBa_B0042

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type: Range
persistent identity: range2410003
display id: range2410003
version: 1
direction: direction/reverse
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start: 3373
end: 3384
orientation: inline

BBa_G00000
type: SequenceAnnotation
persistent identity: annotation2410017
display id: annotation2410017
version: 1
top level: pSB4K50
location: https://synbiohub.org/public/igem/BBa_K1362097/annotation2410017/range2410017/1
component: BBa_G00000

https://synbiohub.org/public/igem/BBa_K1362097/annotation2410017/range2410017/1
type: Range
persistent identity: range2410017
display id: range2410017
version: 1
direction: direction/reverse
top level: pSB4K50
start: 3449
end: 3470
orientation: inline

pSB4C50
type: ComponentDefinition
persistent identity: BBa K1362096
display id: BBa_K1362096
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pSB4C50: Low(?) copy BioBrick cloning/expression backbone carrying Cm resistance
creator: Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena B??scher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Sch??fer, Carolin Schmelas, Silvan Schmitz, Max Waldha
created: 2014-10-07T11:00:00Z
modified: 2015-05-08T01:10:04Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _1738_
m user id: 0
owner id: 22830
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
component: BBa_B0042
component: BBa_G00000
component: BBa_B0044
component: BBa_B0062
component: BBa_G0002
component: BBa_G0003
component: BBa_G00102
component: BBa_P1004
component: BBa_K1362463
component: BBa_G00001
component: BBa_G00100
component: BBa_G0004
component: BBa_B0054
component: BBa_B0055
component: BBa_B0043
component: BBa_I50042
component: BBa_B0053
sequence annotation: BBa_G00102
sequence annotation: BBa_G0004
sequence annotation: BBa_P1004
sequence annotation: BBa_B0042
sequence annotation: BBa_B0043
sequence annotation: BBa_I50042
sequence annotation: BBa_G00001
sequence annotation: BBa_B0062
sequence annotation: BBa_G0003
sequence annotation: BBa_G0002
sequence annotation: BBa_B0053
sequence annotation: BBa_B0044
sequence annotation: BBa_G00000
sequence annotation: BBa_K1362463
sequence annotation: BBa_B0054
sequence annotation: BBa_B0055
sequence annotation: BBa_G00100
sequence: https://synbiohub.org/public/igem/BBa_K1362096_sequence/1

BBa_B0042
type: Component
persistent identity: component2409918
display id: component2409918
version: 1
top level: pSB4C50
access: public
definition: https://synbiohub.org/public/igem/BBa_B0042/1

BBa_G00000
type: Component
persistent identity: component2409932
display id: component2409932
version: 1
top level: pSB4C50
access: public
definition: https://synbiohub.org/public/igem/BBa_G00000/1

BBa_B0042
type: Component
persistent identity: component2409864
display id: component2409864
version: 1
top level: pSB4C50
access: public
definition: https://synbiohub.org/public/igem/BBa_B0042/1

BBa_B0044
type: Component
persistent identity: component2409857
display id: component2409857
version: 1
top level: pSB4C50
access: public
definition: https://synbiohub.org/public/igem/BBa_B0044/1

BBa_B0062
type: Component
persistent identity: component2409871
display id: component2409871
version: 1
top level: pSB4C50
access: public
definition: https://synbiohub.org/public/igem/BBa_B0062/1

BBa_G0002
type: Component
persistent identity: component2409901
display id: component2409901
version: 1
top level: pSB4C50
access: public
definition: https://synbiohub.org/public/igem/BBa_G0002/1

BBa_G0003
type: Component
persistent identity: component2409872
display id: component2409872
version: 1
top level: pSB4C50
access: public
definition: https://synbiohub.org/public/igem/BBa_G0003/1

BBa_G00102
type: Component
persistent identity: component2409869
display id: component2409869
version: 1
top level: pSB4C50
access: public
definition: https://synbiohub.org/public/igem/BBa_G00102/1

BBa_P1004
type: Component
persistent identity: component2409903
display id: component2409903
version: 1
top level: pSB4C50
access: public
definition: https://synbiohub.org/public/igem/BBa_P1004/1

BBa_K1362463
type: Component
persistent identity: component2409927
display id: component2409927
version: 1
top level: pSB4C50
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362463/1

BBa_G00001
type: Component
persistent identity: component2409852
display id: component2409852
version: 1
top level: pSB4C50
access: public
definition: https://synbiohub.org/public/igem/BBa_G00001/1

BBa_G00100
type: Component
persistent identity: component2409908
display id: component2409908
version: 1
top level: pSB4C50
access: public
definition: https://synbiohub.org/public/igem/BBa_G00100/1

BBa_G0004
type: Component
persistent identity: component2409904
display id: component2409904
version: 1
top level: pSB4C50
access: public
definition: https://synbiohub.org/public/igem/BBa_G0004/1

BBa_B0054
type: Component
persistent identity: component2409867
display id: component2409867
version: 1
top level: pSB4C50
access: public
definition: https://synbiohub.org/public/igem/BBa_B0054/1

BBa_B0055
type: Component
persistent identity: component2409911
display id: component2409911
version: 1
top level: pSB4C50
access: public
definition: https://synbiohub.org/public/igem/BBa_B0055/1

BBa_B0043
type: Component
persistent identity: component2409923
display id: component2409923
version: 1
top level: pSB4C50
access: public
definition: https://synbiohub.org/public/igem/BBa_B0043/1

BBa_I50042
type: Component
persistent identity: component2409900
display id: component2409900
version: 1
top level: pSB4C50
access: public
definition: https://synbiohub.org/public/igem/BBa_I50042/1

BBa_B0053
type: Component
persistent identity: component2409906
display id: component2409906
version: 1
top level: pSB4C50
access: public
definition: https://synbiohub.org/public/igem/BBa_B0053/1

BBa_G00102
type: SequenceAnnotation
persistent identity: annotation2409869
display id: annotation2409869
version: 1
top level: pSB4C50
location: https://synbiohub.org/public/igem/BBa_K1362096/annotation2409869/range2409869/1
component: BBa_G00102

https://synbiohub.org/public/igem/BBa_K1362096/annotation2409869/range2409869/1
type: Range
persistent identity: range2409869
display id: range2409869
version: 1
direction: direction/reverse
top level: pSB4C50
start: 148
end: 167
orientation: inline

BBa_G0004
type: SequenceAnnotation
persistent identity: annotation2409904
display id: annotation2409904
version: 1
top level: pSB4C50
location: https://synbiohub.org/public/igem/BBa_K1362096/annotation2409904/range2409904/1
component: BBa_G0004

https://synbiohub.org/public/igem/BBa_K1362096/annotation2409904/range2409904/1
type: Range
persistent identity: range2409904
display id: range2409904
version: 1
direction: direction/reverse
top level: pSB4C50
start: 3078
end: 3084
orientation: inline

BBa_P1004
type: SequenceAnnotation
persistent identity: annotation2409903
display id: annotation2409903
version: 1
top level: pSB4C50
location: https://synbiohub.org/public/igem/BBa_K1362096/annotation2409903/range2409903/1
component: BBa_P1004

https://synbiohub.org/public/igem/BBa_K1362096/annotation2409903/range2409903/1
type: Range
persistent identity: range2409903
display id: range2409903
version: 1
direction: direction/reverse
top level: pSB4C50
start: 2301
end: 3069
orientation: inline

BBa_B0042
type: SequenceAnnotation
persistent identity: annotation2409918
display id: annotation2409918
version: 1
top level: pSB4C50
location: https://synbiohub.org/public/igem/BBa_K1362096/annotation2409918/range2409918/1
component: BBa_B0042

https://synbiohub.org/public/igem/BBa_K1362096/annotation2409918/range2409918/1
type: Range
persistent identity: range2409918
display id: range2409918
version: 1
direction: direction/reverse
top level: pSB4C50
start: 3287
end: 3298
orientation: inline

BBa_B0043
type: SequenceAnnotation
persistent identity: annotation2409923
display id: annotation2409923
version: 1
top level: pSB4C50
location: https://synbiohub.org/public/igem/BBa_K1362096/annotation2409923/range2409923/1
component: BBa_B0043

https://synbiohub.org/public/igem/BBa_K1362096/annotation2409923/range2409923/1
type: Range
persistent identity: range2409923
display id: range2409923
version: 1
direction: direction/reverse
top level: pSB4C50
start: 3307
end: 3319
orientation: inline

BBa_I50042
type: SequenceAnnotation
persistent identity: annotation2409900
display id: annotation2409900
version: 1
top level: pSB4C50
location: https://synbiohub.org/public/igem/BBa_K1362096/annotation2409900/range2409900/1
component: BBa_I50042

https://synbiohub.org/public/igem/BBa_K1362096/annotation2409900/range2409900/1
type: Range
persistent identity: range2409900
display id: range2409900
version: 1
direction: direction/reverse
top level: pSB4C50
start: 240
end: 2276
orientation: inline

BBa_G00001
type: SequenceAnnotation
persistent identity: annotation2409852
display id: annotation2409852
version: 1
top level: pSB4C50
location: https://synbiohub.org/public/igem/BBa_K1362096/annotation2409852/range2409852/1
component: BBa_G00001

https://synbiohub.org/public/igem/BBa_K1362096/annotation2409852/range2409852/1
type: Range
persistent identity: range2409852
display id: range2409852
version: 1
direction: direction/reverse
top level: pSB4C50
start: 1
end: 21
orientation: inline

BBa_B0062
type: SequenceAnnotation
persistent identity: annotation2409871
display id: annotation2409871
version: 1
top level: pSB4C50
location: https://synbiohub.org/public/igem/BBa_K1362096/annotation2409871/range2409871/1
component: BBa_B0062

https://synbiohub.org/public/igem/BBa_K1362096/annotation2409871/range2409871/1
type: Range
persistent identity: range2409871
display id: range2409871
version: 1
direction: direction/reverse
top level: pSB4C50
start: 176
end: 216
orientation: inline

BBa_G0003
type: SequenceAnnotation
persistent identity: annotation2409872
display id: annotation2409872
version: 1
top level: pSB4C50
location: https://synbiohub.org/public/igem/BBa_K1362096/annotation2409872/range2409872/1
component: BBa_G0003

https://synbiohub.org/public/igem/BBa_K1362096/annotation2409872/range2409872/1
type: Range
persistent identity: range2409872
display id: range2409872
version: 1
direction: direction/reverse
top level: pSB4C50
start: 225
end: 231
orientation: inline

BBa_G0002
type: SequenceAnnotation
persistent identity: annotation2409901
display id: annotation2409901
version: 1
top level: pSB4C50
location: https://synbiohub.org/public/igem/BBa_K1362096/annotation2409901/range2409901/1
component: BBa_G0002

https://synbiohub.org/public/igem/BBa_K1362096/annotation2409901/range2409901/1
type: Range
persistent identity: range2409901
display id: range2409901
version: 1
direction: direction/reverse
top level: pSB4C50
start: 2285
end: 2292
orientation: inline

BBa_B0053
type: SequenceAnnotation
persistent identity: annotation2409906
display id: annotation2409906
version: 1
top level: pSB4C50
location: https://synbiohub.org/public/igem/BBa_K1362096/annotation2409906/range2409906/1
component: BBa_B0053

https://synbiohub.org/public/igem/BBa_K1362096/annotation2409906/range2409906/1
type: Range
persistent identity: range2409906
display id: range2409906
version: 1
direction: direction/reverse
top level: pSB4C50
start: 3093
end: 3164
orientation: inline

BBa_B0042
type: SequenceAnnotation
persistent identity: annotation2409864
display id: annotation2409864
version: 1
top level: pSB4C50
location: https://synbiohub.org/public/igem/BBa_K1362096/annotation2409864/range2409864/1
component: BBa_B0042

https://synbiohub.org/public/igem/BBa_K1362096/annotation2409864/range2409864/1
type: Range
persistent identity: range2409864
display id: range2409864
version: 1
direction: direction/reverse
top level: pSB4C50
start: 51
end: 62
orientation: inline

BBa_B0044
type: SequenceAnnotation
persistent identity: annotation2409857
display id: annotation2409857
version: 1
top level: pSB4C50
location: https://synbiohub.org/public/igem/BBa_K1362096/annotation2409857/range2409857/1
component: BBa_B0044

https://synbiohub.org/public/igem/BBa_K1362096/annotation2409857/range2409857/1
type: Range
persistent identity: range2409857
display id: range2409857
version: 1
direction: direction/reverse
top level: pSB4C50
start: 30
end: 42
orientation: inline

BBa_G00000
type: SequenceAnnotation
persistent identity: annotation2409932
display id: annotation2409932
version: 1
top level: pSB4C50
location: https://synbiohub.org/public/igem/BBa_K1362096/annotation2409932/range2409932/1
component: BBa_G00000

https://synbiohub.org/public/igem/BBa_K1362096/annotation2409932/range2409932/1
type: Range
persistent identity: range2409932
display id: range2409932
version: 1
direction: direction/reverse
top level: pSB4C50
start: 3387
end: 3408
orientation: inline

BBa_K1362463
type: SequenceAnnotation
persistent identity: annotation2409927
display id: annotation2409927
version: 1
top level: pSB4C50
location: https://synbiohub.org/public/igem/BBa_K1362096/annotation2409927/range2409927/1
component: BBa_K1362463

https://synbiohub.org/public/igem/BBa_K1362096/annotation2409927/range2409927/1
type: Range
persistent identity: range2409927
display id: range2409927
version: 1
direction: direction/reverse
top level: pSB4C50
start: 3328
end: 3378
orientation: inline

BBa_B0054
type: SequenceAnnotation
persistent identity: annotation2409867
display id: annotation2409867
version: 1
top level: pSB4C50
location: https://synbiohub.org/public/igem/BBa_K1362096/annotation2409867/range2409867/1
component: BBa_B0054

https://synbiohub.org/public/igem/BBa_K1362096/annotation2409867/range2409867/1
type: Range
persistent identity: range2409867
display id: range2409867
version: 1
direction: direction/reverse
top level: pSB4C50
start: 71
end: 139
orientation: inline

BBa_B0055
type: SequenceAnnotation
persistent identity: annotation2409911
display id: annotation2409911
version: 1
top level: pSB4C50
location: https://synbiohub.org/public/igem/BBa_K1362096/annotation2409911/range2409911/1
component: BBa_B0055

https://synbiohub.org/public/igem/BBa_K1362096/annotation2409911/range2409911/1
type: Range
persistent identity: range2409911
display id: range2409911
version: 1
direction: direction/reverse
top level: pSB4C50
start: 3201
end: 3278
orientation: inline

BBa_G00100
type: SequenceAnnotation
persistent identity: annotation2409908
display id: annotation2409908
version: 1
top level: pSB4C50
location: https://synbiohub.org/public/igem/BBa_K1362096/annotation2409908/range2409908/1
component: BBa_G00100

https://synbiohub.org/public/igem/BBa_K1362096/annotation2409908/range2409908/1
type: Range
persistent identity: range2409908
display id: range2409908
version: 1
direction: direction/reverse
top level: pSB4C50
start: 3173
end: 3192
orientation: inline

pSB4T5
type: ComponentDefinition
persistent identity: pSB4T5
display id: pSB4T5
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Low copy BioBrick standard vector
creator: Reshma Shetty
created: 2007-02-28T12:00:00Z
modified: 2015-05-08T01:14:55Z
discontinued: false
dominant: false
experience: experience/None
group u list: _41_
m user id: 0
owner id: 126
owning group id: 70
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: pSB4T3 is a low copy plasmid with tetracycline resistance. It has a repA pSC101-derived replication origin. (Copy number ~10-12, Lutz and Bujard, 1997.) It is constructed from the BioBricks vector scaffold BBa_I51001.
mutable notes: See http://openwetware.org/wiki/Synthetic_Biology:Vectors/pSB%2A%2A5_design for more information.
mutable provenance: BBa_I51001 vector scaffold, BBa_I50040 replication origin and BBa_P1005 resistance marker.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
component: BBa_B0054
component: BBa_G00102
component: BBa_G0004
component: BBa_G00001
component: BBa_B0042
component: BBa_I50042
component: BBa_G0002
component: BBa_B0043
component: BBa_B0055
component: BBa_B0053
component: BBa_G00000
component: BBa_G0003
component: BBa_G00100
component: BBa_B0062
component: BBa_B0044
component: BBa_P1005
sequence annotation: BBa_G00000
sequence annotation: BBa_G00001
sequence annotation: BBa_B0042
sequence annotation: BBa_B0062
sequence annotation: BBa_P1005
sequence annotation: BBa_G00102
sequence annotation: BBa_I50042
sequence annotation: BBa_B0043
sequence annotation: BBa_G0002
sequence annotation: BBa_G00100
sequence annotation: BBa_G0004
sequence annotation: BBa_B0053
sequence annotation: BBa_B0055
sequence annotation: BBa_G0003
sequence annotation: BBa_B0054
sequence annotation: BBa_B0044
sequence: https://synbiohub.org/public/igem/pSB4T5_sequence/1

BBa_B0054
type: Component
persistent identity: component2236416
display id: component2236416
version: 1
top level: pSB4T5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0054/1

BBa_G00102
type: Component
persistent identity: component2236418
display id: component2236418
version: 1
top level: pSB4T5
access: public
definition: https://synbiohub.org/public/igem/BBa_G00102/1

BBa_G0004
type: Component
persistent identity: component2236454
display id: component2236454
version: 1
top level: pSB4T5
access: public
definition: https://synbiohub.org/public/igem/BBa_G0004/1

BBa_G00001
type: Component
persistent identity: component2236401
display id: component2236401
version: 1
top level: pSB4T5
access: public
definition: https://synbiohub.org/public/igem/BBa_G00001/1

BBa_B0042
type: Component
persistent identity: component2236413
display id: component2236413
version: 1
top level: pSB4T5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0042/1

BBa_I50042
type: Component
persistent identity: component2236449
display id: component2236449
version: 1
top level: pSB4T5
access: public
definition: https://synbiohub.org/public/igem/BBa_I50042/1

BBa_G0002
type: Component
persistent identity: component2236450
display id: component2236450
version: 1
top level: pSB4T5
access: public
definition: https://synbiohub.org/public/igem/BBa_G0002/1

BBa_B0042
type: Component
persistent identity: component2236468
display id: component2236468
version: 1
top level: pSB4T5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0042/1

BBa_B0043
type: Component
persistent identity: component2236473
display id: component2236473
version: 1
top level: pSB4T5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0043/1

BBa_B0055
type: Component
persistent identity: component2236461
display id: component2236461
version: 1
top level: pSB4T5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0055/1

BBa_B0053
type: Component
persistent identity: component2236456
display id: component2236456
version: 1
top level: pSB4T5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0053/1

BBa_G00000
type: Component
persistent identity: component2236478
display id: component2236478
version: 1
top level: pSB4T5
access: public
definition: https://synbiohub.org/public/igem/BBa_G00000/1

BBa_G0003
type: Component
persistent identity: component2236421
display id: component2236421
version: 1
top level: pSB4T5
access: public
definition: https://synbiohub.org/public/igem/BBa_G0003/1

BBa_G00100
type: Component
persistent identity: component2236458
display id: component2236458
version: 1
top level: pSB4T5
access: public
definition: https://synbiohub.org/public/igem/BBa_G00100/1

BBa_B0062
type: Component
persistent identity: component2236420
display id: component2236420
version: 1
top level: pSB4T5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0062/1

BBa_B0044
type: Component
persistent identity: component2236406
display id: component2236406
version: 1
top level: pSB4T5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0044/1

BBa_P1005
type: Component
persistent identity: component2236453
display id: component2236453
version: 1
top level: pSB4T5
access: public
definition: https://synbiohub.org/public/igem/BBa_P1005/1

BBa_G00000
type: SequenceAnnotation
persistent identity: annotation2236478
display id: annotation2236478
version: 1
top level: pSB4T5
location: https://synbiohub.org/public/igem/pSB4T5/annotation2236478/range2236478/1
component: BBa_G00000

https://synbiohub.org/public/igem/pSB4T5/annotation2236478/range2236478/1
type: Range
persistent identity: range2236478
display id: range2236478
version: 1
direction: direction/reverse
top level: pSB4T5
start: 3714
end: 3735
orientation: inline

BBa_G00001
type: SequenceAnnotation
persistent identity: annotation2236401
display id: annotation2236401
version: 1
top level: pSB4T5
location: https://synbiohub.org/public/igem/pSB4T5/annotation2236401/range2236401/1
component: BBa_G00001

https://synbiohub.org/public/igem/pSB4T5/annotation2236401/range2236401/1
type: Range
persistent identity: range2236401
display id: range2236401
version: 1
direction: direction/reverse
top level: pSB4T5
start: 1
end: 21
orientation: inline

BBa_B0042
type: SequenceAnnotation
persistent identity: annotation2236468
display id: annotation2236468
version: 1
top level: pSB4T5
location: https://synbiohub.org/public/igem/pSB4T5/annotation2236468/range2236468/1
component: BBa_B0042

https://synbiohub.org/public/igem/pSB4T5/annotation2236468/range2236468/1
type: Range
persistent identity: range2236468
display id: range2236468
version: 1
direction: direction/reverse
top level: pSB4T5
start: 3689
end: 3700
orientation: inline

BBa_B0062
type: SequenceAnnotation
persistent identity: annotation2236420
display id: annotation2236420
version: 1
top level: pSB4T5
location: https://synbiohub.org/public/igem/pSB4T5/annotation2236420/range2236420/1
component: BBa_B0062

https://synbiohub.org/public/igem/pSB4T5/annotation2236420/range2236420/1
type: Range
persistent identity: range2236420
display id: range2236420
version: 1
direction: direction/reverse
top level: pSB4T5
start: 136
end: 176
orientation: inline

BBa_B0042
type: SequenceAnnotation
persistent identity: annotation2236413
display id: annotation2236413
version: 1
top level: pSB4T5
location: https://synbiohub.org/public/igem/pSB4T5/annotation2236413/range2236413/1
component: BBa_B0042

https://synbiohub.org/public/igem/pSB4T5/annotation2236413/range2236413/1
type: Range
persistent identity: range2236413
display id: range2236413
version: 1
direction: direction/reverse
top level: pSB4T5
start: 35
end: 46
orientation: inline

BBa_P1005
type: SequenceAnnotation
persistent identity: annotation2236453
display id: annotation2236453
version: 1
top level: pSB4T5
location: https://synbiohub.org/public/igem/pSB4T5/annotation2236453/range2236453/1
component: BBa_P1005

https://synbiohub.org/public/igem/pSB4T5/annotation2236453/range2236453/1
type: Range
persistent identity: range2236453
display id: range2236453
version: 1
direction: direction/reverse
top level: pSB4T5
start: 2229
end: 3511
orientation: inline

BBa_G00102
type: SequenceAnnotation
persistent identity: annotation2236418
display id: annotation2236418
version: 1
top level: pSB4T5
location: https://synbiohub.org/public/igem/pSB4T5/annotation2236418/range2236418/1
component: BBa_G00102

https://synbiohub.org/public/igem/pSB4T5/annotation2236418/range2236418/1
type: Range
persistent identity: range2236418
display id: range2236418
version: 1
direction: direction/reverse
top level: pSB4T5
start: 116
end: 135
orientation: inline

BBa_I50042
type: SequenceAnnotation
persistent identity: annotation2236449
display id: annotation2236449
version: 1
top level: pSB4T5
location: https://synbiohub.org/public/igem/pSB4T5/annotation2236449/range2236449/1
component: BBa_I50042

https://synbiohub.org/public/igem/pSB4T5/annotation2236449/range2236449/1
type: Range
persistent identity: range2236449
display id: range2236449
version: 1
direction: direction/reverse
top level: pSB4T5
start: 184
end: 2220
orientation: inline

BBa_B0043
type: SequenceAnnotation
persistent identity: annotation2236473
display id: annotation2236473
version: 1
top level: pSB4T5
location: https://synbiohub.org/public/igem/pSB4T5/annotation2236473/range2236473/1
component: BBa_B0043

https://synbiohub.org/public/igem/pSB4T5/annotation2236473/range2236473/1
type: Range
persistent identity: range2236473
display id: range2236473
version: 1
direction: direction/reverse
top level: pSB4T5
start: 3701
end: 3713
orientation: inline

BBa_G0002
type: SequenceAnnotation
persistent identity: annotation2236450
display id: annotation2236450
version: 1
top level: pSB4T5
location: https://synbiohub.org/public/igem/pSB4T5/annotation2236450/range2236450/1
component: BBa_G0002

https://synbiohub.org/public/igem/pSB4T5/annotation2236450/range2236450/1
type: Range
persistent identity: range2236450
display id: range2236450
version: 1
direction: direction/reverse
top level: pSB4T5
start: 2221
end: 2228
orientation: inline

BBa_G00100
type: SequenceAnnotation
persistent identity: annotation2236458
display id: annotation2236458
version: 1
top level: pSB4T5
location: https://synbiohub.org/public/igem/pSB4T5/annotation2236458/range2236458/1
component: BBa_G00100

https://synbiohub.org/public/igem/pSB4T5/annotation2236458/range2236458/1
type: Range
persistent identity: range2236458
display id: range2236458
version: 1
direction: direction/reverse
top level: pSB4T5
start: 3591
end: 3610
orientation: inline

BBa_G0004
type: SequenceAnnotation
persistent identity: annotation2236454
display id: annotation2236454
version: 1
top level: pSB4T5
location: https://synbiohub.org/public/igem/pSB4T5/annotation2236454/range2236454/1
component: BBa_G0004

https://synbiohub.org/public/igem/pSB4T5/annotation2236454/range2236454/1
type: Range
persistent identity: range2236454
display id: range2236454
version: 1
direction: direction/reverse
top level: pSB4T5
start: 3512
end: 3518
orientation: inline

BBa_B0053
type: SequenceAnnotation
persistent identity: annotation2236456
display id: annotation2236456
version: 1
top level: pSB4T5
location: https://synbiohub.org/public/igem/pSB4T5/annotation2236456/range2236456/1
component: BBa_B0053

https://synbiohub.org/public/igem/pSB4T5/annotation2236456/range2236456/1
type: Range
persistent identity: range2236456
display id: range2236456
version: 1
direction: direction/reverse
top level: pSB4T5
start: 3519
end: 3590
orientation: inline

BBa_B0055
type: SequenceAnnotation
persistent identity: annotation2236461
display id: annotation2236461
version: 1
top level: pSB4T5
location: https://synbiohub.org/public/igem/pSB4T5/annotation2236461/range2236461/1
component: BBa_B0055

https://synbiohub.org/public/igem/pSB4T5/annotation2236461/range2236461/1
type: Range
persistent identity: range2236461
display id: range2236461
version: 1
direction: direction/reverse
top level: pSB4T5
start: 3611
end: 3688
orientation: inline

BBa_G0003
type: SequenceAnnotation
persistent identity: annotation2236421
display id: annotation2236421
version: 1
top level: pSB4T5
location: https://synbiohub.org/public/igem/pSB4T5/annotation2236421/range2236421/1
component: BBa_G0003

https://synbiohub.org/public/igem/pSB4T5/annotation2236421/range2236421/1
type: Range
persistent identity: range2236421
display id: range2236421
version: 1
direction: direction/reverse
top level: pSB4T5
start: 177
end: 183
orientation: inline

BBa_B0054
type: SequenceAnnotation
persistent identity: annotation2236416
display id: annotation2236416
version: 1
top level: pSB4T5
location: https://synbiohub.org/public/igem/pSB4T5/annotation2236416/range2236416/1
component: BBa_B0054

https://synbiohub.org/public/igem/pSB4T5/annotation2236416/range2236416/1
type: Range
persistent identity: range2236416
display id: range2236416
version: 1
direction: direction/reverse
top level: pSB4T5
start: 47
end: 115
orientation: inline

BBa_B0044
type: SequenceAnnotation
persistent identity: annotation2236406
display id: annotation2236406
version: 1
top level: pSB4T5
location: https://synbiohub.org/public/igem/pSB4T5/annotation2236406/range2236406/1
component: BBa_B0044

https://synbiohub.org/public/igem/pSB4T5/annotation2236406/range2236406/1
type: Range
persistent identity: range2236406
display id: range2236406
version: 1
direction: direction/reverse
top level: pSB4T5
start: 22
end: 34
orientation: inline

pSac-Cm_B
type: ComponentDefinition
persistent identity: BBa K1185004
display id: BBa_K1185004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Integration vector for Bacillus subtilis derived from pSac-Cm
creator: Vincent Leonardo
created: 2013-10-03T11:00:00Z
modified: 2015-05-08T01:09:37Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1498_
m user id: 0
owner id: 16794
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This plasmid was derived from pSac-Cm by insertion of biobrick compatible restriction sites (prefixes and suffixes), a terminator (BBa_B0015) after the suffixes sequences, and red fluorescent protein sequence (RFP) in between the prefix and suffix in its multiple cloning sites (MCS). New biobricks can be inserted into this vector by replacement of the RFP biobrick, and selection of the white colonies. This backbone has a multi host replication origin and replicates in E. coli. The plasmid is designed to integrate a cloned insert into the B. subtilis chromosome via double recombination between plasmid and chromosomal sacA sequences, and in order to check for integration a simple Phenol Red Sucrose test can be perform, if sacA is no longer functional, they will remain ph7, if they are still able to digest sucrose their pH will be under 5.
mutable notes: We found that that the integration plasmid that groningen send to the registry were unable to integrate in B. subtilis. This is due to the high number of mutations in the sacA integration region.
mutable provenance: Groningen iGEM 2012 team
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: 'sacA
sequence annotation: cat
sequence annotation: B0015
sequence annotation: bla
sequence: https://synbiohub.org/public/igem/BBa_K1185004_sequence/1

'sacA
type: SequenceAnnotation
persistent identity: annotation2367616
display id: annotation2367616
version: 1
top level: pSac-Cm_B
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1185004/annotation2367616/range2367616/1

https://synbiohub.org/public/igem/BBa_K1185004/annotation2367616/range2367616/1
type: Range
persistent identity: range2367616
display id: range2367616
version: 1
direction: direction/forward
top level: pSac-Cm_B
start: 3167
end: 3922
orientation: inline

'sacA
type: SequenceAnnotation
persistent identity: annotation2367614
display id: annotation2367614
version: 1
top level: pSac-Cm_B
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1185004/annotation2367614/range2367614/1

https://synbiohub.org/public/igem/BBa_K1185004/annotation2367614/range2367614/1
type: Range
persistent identity: range2367614
display id: range2367614
version: 1
direction: direction/forward
top level: pSac-Cm_B
start: 167
end: 970
orientation: inline

cat
type: SequenceAnnotation
persistent identity: annotation2367617
display id: annotation2367617
version: 1
top level: pSac-Cm_B
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1185004/annotation2367617/range2367617/1

https://synbiohub.org/public/igem/BBa_K1185004/annotation2367617/range2367617/1
type: Range
persistent identity: range2367617
display id: range2367617
version: 1
direction: direction/forward
top level: pSac-Cm_B
start: 4231
end: 4894
orientation: inline

B0015
type: SequenceAnnotation
persistent identity: annotation2367613
display id: annotation2367613
version: 1
top level: pSac-Cm_B
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1185004/annotation2367613/range2367613/1

https://synbiohub.org/public/igem/BBa_K1185004/annotation2367613/range2367613/1
type: Range
persistent identity: range2367613
display id: range2367613
version: 1
direction: direction/forward
top level: pSac-Cm_B
start: 23
end: 151
orientation: inline

bla
type: SequenceAnnotation
persistent identity: annotation2367615
display id: annotation2367615
version: 1
top level: pSac-Cm_B
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1185004/annotation2367615/range2367615/1

https://synbiohub.org/public/igem/BBa_K1185004/annotation2367615/range2367615/1
type: Range
persistent identity: range2367615
display id: range2367615
version: 1
direction: direction/forward
top level: pSac-Cm_B
start: 1306
end: 2176
orientation: inline

pSB3K1
type: ComponentDefinition
persistent identity: pSB3K1
display id: pSB3K1
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pACYC177 converted into BioBrick vector (formerly BBa_I739202)
creator: Stefan Luzi
created: 2007-10-22T11:00:00Z
modified: 2015-05-08T01:14:55Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _124_
m user id: 0
owner id: 2183
owning group id: 62
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: asdf
mutable notes: asdf
mutable provenance: asdf
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: partial Tn903 repressor fragment
sequence annotation: BioBrick suffix
sequence annotation: p15A replication origin
sequence annotation: Tn903 inverted repeat
sequence annotation: Tn3 inverted repeat
sequence annotation: partial ampicillin resistance marker (nonfunctional)
sequence annotation: kanamycin resistance marker
sequence annotation: BioBrick prefix
sequence: https://synbiohub.org/public/igem/pSB3K1_sequence/1

partial Tn903 repressor fragment
type: SequenceAnnotation
persistent identity: annotation1969628
display id: annotation1969628
version: 1
top level: pSB3K1
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB3K1/annotation1969628/range1969628/1

https://synbiohub.org/public/igem/pSB3K1/annotation1969628/range1969628/1
type: Range
persistent identity: range1969628
display id: range1969628
version: 1
direction: direction/forward
top level: pSB3K1
start: 2742
end: 3036
orientation: inline

BioBrick suffix
type: SequenceAnnotation
persistent identity: annotation1969629
display id: annotation1969629
version: 1
top level: pSB3K1
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/pSB3K1/annotation1969629/range1969629/1

https://synbiohub.org/public/igem/pSB3K1/annotation1969629/range1969629/1
type: Range
persistent identity: range1969629
display id: range1969629
version: 1
direction: direction/forward
top level: pSB3K1
start: 1
end: 21
orientation: inline

p15A replication origin
type: SequenceAnnotation
persistent identity: annotation1969622
display id: annotation1969622
version: 1
top level: pSB3K1
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB3K1/annotation1969622/range1969622/1

https://synbiohub.org/public/igem/pSB3K1/annotation1969622/range1969622/1
type: Range
persistent identity: range1969622
display id: range1969622
version: 1
direction: direction/forward
top level: pSB3K1
start: 483
end: 1312
orientation: inline

Tn903 inverted repeat
type: SequenceAnnotation
persistent identity: annotation1969627
display id: annotation1969627
version: 1
top level: pSB3K1
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/pSB3K1/annotation1969627/range1969627/1

https://synbiohub.org/public/igem/pSB3K1/annotation1969627/range1969627/1
type: Range
persistent identity: range1969627
display id: range1969627
version: 1
direction: direction/forward
top level: pSB3K1
start: 2515
end: 2742
orientation: inline

Tn3 inverted repeat
type: SequenceAnnotation
persistent identity: annotation1969625
display id: annotation1969625
version: 1
top level: pSB3K1
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/pSB3K1/annotation1969625/range1969625/1

https://synbiohub.org/public/igem/pSB3K1/annotation1969625/range1969625/1
type: Range
persistent identity: range1969625
display id: range1969625
version: 1
direction: direction/forward
top level: pSB3K1
start: 445
end: 482
orientation: inline

partial ampicillin resistance marker (nonfunctional)
type: SequenceAnnotation
persistent identity: annotation1969624
display id: annotation1969624
version: 1
top level: pSB3K1
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB3K1/annotation1969624/range1969624/1

https://synbiohub.org/public/igem/pSB3K1/annotation1969624/range1969624/1
type: Range
persistent identity: range1969624
display id: range1969624
version: 1
direction: direction/forward
top level: pSB3K1
start: 22
end: 332
orientation: inline

kanamycin resistance marker
type: SequenceAnnotation
persistent identity: annotation1969623
display id: annotation1969623
version: 1
top level: pSB3K1
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB3K1/annotation1969623/range1969623/1

https://synbiohub.org/public/igem/pSB3K1/annotation1969623/range1969623/1
type: Range
persistent identity: range1969623
display id: range1969623
version: 1
direction: direction/forward
top level: pSB3K1
start: 1637
end: 2453
orientation: inline

Tn903 inverted repeat
type: SequenceAnnotation
persistent identity: annotation1969626
display id: annotation1969626
version: 1
top level: pSB3K1
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/pSB3K1/annotation1969626/range1969626/1

https://synbiohub.org/public/igem/pSB3K1/annotation1969626/range1969626/1
type: Range
persistent identity: range1969626
display id: range1969626
version: 1
direction: direction/forward
top level: pSB3K1
start: 1307
end: 1534
orientation: inline

BioBrick prefix
type: SequenceAnnotation
persistent identity: annotation1969630
display id: annotation1969630
version: 1
top level: pSB3K1
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/pSB3K1/annotation1969630/range1969630/1

https://synbiohub.org/public/igem/pSB3K1/annotation1969630/range1969630/1
type: Range
persistent identity: range1969630
display id: range1969630
version: 1
direction: direction/forward
top level: pSB3K1
start: 3043
end: 3064
orientation: inline

BBa_K864011
type: ComponentDefinition
persistent identity: BBa K864011
display id: BBa_K864011
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Low copy BioBrick standard vector with LacI
creator: Erik Lundin
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:37Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1124_
m user id: 0
owner id: 9827
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: pSB4A15Iq is a BioBrick standard vector with low copy pSC101 replication origin (~5 copies per cell) and ampicillin antibiotic resistance marker, especially usable for Lambda Red recombineering in E coli. The backbone sequence is based on pSB3T5, but the E coli His operon terminator BBa_B0053 has been replaced with the late terminator of the Salmonella phage P22. This vector carries a LacIq cassette on the backbone for tight repression of any lac promoter cloned into the cloning site.
mutable notes: The resistance cassette is terminated by a Lux bidirectional terminator. Adjacent to this terminator there is a designed primer binding site to where the forward primer for lambda red can be designed to bind to no matter what the insert in the cloning site is or what the resistance is. The resistance cassette is flanked by SalI and SacI restriction sites for easy switching of resistance in the backbone.
mutable provenance: Based on BBa_K864003
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence: https://synbiohub.org/public/igem/BBa_K864011_sequence/1

Adh1P Acce
type: ComponentDefinition
persistent identity: BBa K106006
display id: BBa_K106006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: AarI AD acceptor vector (pRS315, Adh1P, Adh1t)
creator: Sergio Peisajovich
created: 2008-10-22T11:00:00Z
modified: 2015-05-08T01:08:58Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _226_
m user id: 0
owner id: 2659
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: This is an acceptor vector for AarI cloning, with A and D ends. Cloning into this plasmid will put your parts under the control of a Adh1 promoter (Adh1P) and Adh1 terminator (Adh1t). For more information regarding AarI acceptor vectors, and AarI cloning in general, please visit the UCSF 2008 wiki.
mutable notes: The pRS315 vector has been modified with an AarI cloning cassette inserted between the PspOMI and SacI sites.
mutable provenance: A modified pRS315 vector.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: Stops in all 3 frames
sequence annotation: T7 promoter
sequence annotation: Adh1 Promoter
sequence annotation: pBR322 origin
sequence annotation: ampicillin resistance marker
sequence annotation: f1 origin
sequence annotation: D overhang
sequence annotation: T3 promoter
sequence annotation: AarI AD MCS
sequence annotation: CEN ARS
sequence annotation: lac promoter
sequence annotation: Adh1 terminator
sequence annotation: A overhang
sequence annotation: LEU2 marker
sequence annotation: ampicillin resistance promoter
sequence annotation: ATG start codon
sequence annotation: lacZalpha reporter
sequence: https://synbiohub.org/public/igem/BBa_K106006_sequence/1

Stops in all 3 frames
type: SequenceAnnotation
persistent identity: annotation1999672
display id: annotation1999672
version: 1
top level: Adh1P Acce
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K106006/annotation1999672/range1999672/1

https://synbiohub.org/public/igem/BBa_K106006/annotation1999672/range1999672/1
type: Range
persistent identity: range1999672
display id: range1999672
version: 1
direction: direction/forward
top level: Adh1P Acce
start: 11
end: 21
orientation: inline

T7 promoter
type: SequenceAnnotation
persistent identity: annotation1999674
display id: annotation1999674
version: 1
top level: Adh1P Acce
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K106006/annotation1999674/range1999674/1

https://synbiohub.org/public/igem/BBa_K106006/annotation1999674/range1999674/1
type: Range
persistent identity: range1999674
display id: range1999674
version: 1
direction: direction/reverse
top level: Adh1P Acce
start: 224
end: 242
orientation: inline

Adh1 Promoter
type: SequenceAnnotation
persistent identity: annotation1986773
display id: annotation1986773
version: 1
top level: Adh1P Acce
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K106006/annotation1986773/range1986773/1

https://synbiohub.org/public/igem/BBa_K106006/annotation1986773/range1986773/1
type: Range
persistent identity: range1986773
display id: range1986773
version: 1
direction: direction/forward
top level: Adh1P Acce
start: 6132
end: 7646
orientation: inline

pBR322 origin
type: SequenceAnnotation
persistent identity: annotation1999678
display id: annotation1999678
version: 1
top level: Adh1P Acce
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K106006/annotation1999678/range1999678/1

https://synbiohub.org/public/igem/BBa_K106006/annotation1999678/range1999678/1
type: Range
persistent identity: range1999678
display id: range1999678
version: 1
direction: direction/forward
top level: Adh1P Acce
start: 5068
end: 5687
orientation: inline

ampicillin resistance marker
type: SequenceAnnotation
persistent identity: annotation1986805
display id: annotation1986805
version: 1
top level: Adh1P Acce
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K106006/annotation1986805/range1986805/1

https://synbiohub.org/public/igem/BBa_K106006/annotation1986805/range1986805/1
type: Range
persistent identity: range1986805
display id: range1986805
version: 1
direction: direction/forward
top level: Adh1P Acce
start: 4053
end: 4911
orientation: inline

f1 origin
type: SequenceAnnotation
persistent identity: annotation1999676
display id: annotation1999676
version: 1
top level: Adh1P Acce
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K106006/annotation1999676/range1999676/1

https://synbiohub.org/public/igem/BBa_K106006/annotation1999676/range1999676/1
type: Range
persistent identity: range1999676
display id: range1999676
version: 1
direction: direction/forward
top level: Adh1P Acce
start: 427
end: 733
orientation: inline

D overhang
type: SequenceAnnotation
persistent identity: annotation1999682
display id: annotation1999682
version: 1
top level: Adh1P Acce
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K106006/annotation1999682/range1999682/1

https://synbiohub.org/public/igem/BBa_K106006/annotation1999682/range1999682/1
type: Range
persistent identity: range1999682
display id: range1999682
version: 1
direction: direction/forward
top level: Adh1P Acce
start: 1
end: 4
orientation: inline

T3 promoter
type: SequenceAnnotation
persistent identity: annotation1999680
display id: annotation1999680
version: 1
top level: Adh1P Acce
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K106006/annotation1999680/range1999680/1

https://synbiohub.org/public/igem/BBa_K106006/annotation1999680/range1999680/1
type: Range
persistent identity: range1999680
display id: range1999680
version: 1
direction: direction/forward
top level: Adh1P Acce
start: 6095
end: 6114
orientation: inline

AarI AD MCS
type: SequenceAnnotation
persistent identity: annotation1986789
display id: annotation1986789
version: 1
top level: Adh1P Acce
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K106006/annotation1986789/range1986789/1

https://synbiohub.org/public/igem/BBa_K106006/annotation1986789/range1986789/1
type: Range
persistent identity: range1986789
display id: range1986789
version: 1
direction: direction/forward
top level: Adh1P Acce
start: 4309
end: 4385
orientation: inline

CEN ARS
type: SequenceAnnotation
persistent identity: annotation1986790
display id: annotation1986790
version: 1
top level: Adh1P Acce
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K106006/annotation1986790/range1986790/1

https://synbiohub.org/public/igem/BBa_K106006/annotation1986790/range1986790/1
type: Range
persistent identity: range1986790
display id: range1986790
version: 1
direction: direction/reverse
top level: Adh1P Acce
start: 3403
end: 3921
orientation: inline

lac promoter
type: SequenceAnnotation
persistent identity: annotation1999679
display id: annotation1999679
version: 1
top level: Adh1P Acce
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K106006/annotation1999679/range1999679/1

https://synbiohub.org/public/igem/BBa_K106006/annotation1999679/range1999679/1
type: Range
persistent identity: range1999679
display id: range1999679
version: 1
direction: direction/forward
top level: Adh1P Acce
start: 5996
end: 6025
orientation: inline

Adh1 terminator
type: SequenceAnnotation
persistent identity: annotation1986788
display id: annotation1986788
version: 1
top level: Adh1P Acce
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K106006/annotation1986788/range1986788/1

https://synbiohub.org/public/igem/BBa_K106006/annotation1986788/range1986788/1
type: Range
persistent identity: range1986788
display id: range1986788
version: 1
direction: direction/forward
top level: Adh1P Acce
start: 22
end: 215
orientation: inline

A overhang
type: SequenceAnnotation
persistent identity: annotation1999681
display id: annotation1999681
version: 1
top level: Adh1P Acce
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K106006/annotation1999681/range1999681/1

https://synbiohub.org/public/igem/BBa_K106006/annotation1999681/range1999681/1
type: Range
persistent identity: range1999681
display id: range1999681
version: 1
direction: direction/forward
top level: Adh1P Acce
start: 7647
end: 7650
orientation: inline

LEU2 marker
type: SequenceAnnotation
persistent identity: annotation1986791
display id: annotation1986791
version: 1
top level: Adh1P Acce
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K106006/annotation1986791/range1986791/1

https://synbiohub.org/public/igem/BBa_K106006/annotation1986791/range1986791/1
type: Range
persistent identity: range1986791
display id: range1986791
version: 1
direction: direction/forward
top level: Adh1P Acce
start: 1570
end: 2676
orientation: inline

ampicillin resistance promoter
type: SequenceAnnotation
persistent identity: annotation1999677
display id: annotation1999677
version: 1
top level: Adh1P Acce
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K106006/annotation1999677/range1999677/1

https://synbiohub.org/public/igem/BBa_K106006/annotation1999677/range1999677/1
type: Range
persistent identity: range1999677
display id: range1999677
version: 1
direction: direction/forward
top level: Adh1P Acce
start: 3983
end: 4011
orientation: inline

ATG start codon
type: SequenceAnnotation
persistent identity: annotation1999673
display id: annotation1999673
version: 1
top level: Adh1P Acce
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K106006/annotation1999673/range1999673/1

https://synbiohub.org/public/igem/BBa_K106006/annotation1999673/range1999673/1
type: Range
persistent identity: range1999673
display id: range1999673
version: 1
direction: direction/forward
top level: Adh1P Acce
start: 7638
end: 7640
orientation: inline

lacZalpha reporter
type: SequenceAnnotation
persistent identity: annotation1999675
display id: annotation1999675
version: 1
top level: Adh1P Acce
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K106006/annotation1999675/range1999675/1

https://synbiohub.org/public/igem/BBa_K106006/annotation1999675/range1999675/1
type: Range
persistent identity: range1999675
display id: range1999675
version: 1
direction: direction/forward
top level: Adh1P Acce
start: 246
end: 405
orientation: inline

BBa_K864007
type: ComponentDefinition
persistent identity: BBa K864007
display id: BBa_K864007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Low copy BioBrick standard vector
creator: Erik Lundin
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:37Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1124_
m user id: 0
owner id: 9827
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: pSB4S15(FRT) is a BioBrick standard vector with low copy pSC101 replication origin (~5 copies per cell) and spectinomycin antibiotic resistance marker, especially usable for Lambda Red recombineering in E coli. The backbone sequence is based on pSB3T5, but the E coli His operon terminator BBa_B0053 has been replaced with the late terminator of the Salmonella phage P22.
mutable notes: The resistance cassette is terminated by a Lux bidirectional terminator. Adjacent to this terminator there is a designed primer binding site to where the forward primer for lambda red can be designed to bind to no matter what the insert in the cloning site is or what the resistance is. The resistance cassette is flanked by SalI and SacI restriction sites for easy switching of resistance in the backbone. The pSC101 origin of replication is flaked by NheI and MluI restriction sites for easy switching of origin of replication.
mutable provenance: Based on pSB3T5
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence: https://synbiohub.org/public/igem/BBa_K864007_sequence/1

BBa_K864005
type: ComponentDefinition
persistent identity: BBa K864005
display id: BBa_K864005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Low copy BioBrick standard vector
creator: Erik Lundin
created: 2012-09-22T11:00:00Z
modified: 2015-05-08T01:13:37Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1124_
m user id: 0
owner id: 9827
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: pSB4A15 is a BioBrick standard vector with low copy pSC101 replication origin (~5 copies per cell) and ampicillin antibiotic resistance marker, especially usable for Lambda Red recombineering in E coli. The backbone sequence is based on pSB3T5, but the E coli His operon terminator BBa_B0053 has been replaced with the late terminator of the Salmonella phage P22. The resistance cassette is flanked by FRT sites (BBa_J61020) which allows for subsequent removal of the resistance cassette from the chromosome by the FLP recombinase. The pSB8x15 series are especially suitable for carrying the FLP recombinase, since they can be removed by incubation at 42??C.
mutable notes: The resistance cassette is terminated by a Lux bidirectional terminator. Adjacent to this terminator there is a designed primer binding site to where the forward primer for lambda red can be designed to bind to no matter what the insert in the cloning site is or what the resistance is. The resistance cassette is flanked by SalI and SacI restriction sites for easy switching of resistance in the backbone. The pSC101 origin of replication is flaked by NheI and MluI restriction sites for easy switching of origin of replication.
mutable provenance: Based on pSB3T5
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: Lux terminator
sequence annotation: Lambda Red forward primer
sequence annotation: pSC101 ori BBa_K864050
sequence annotation: rrnC terminator
sequence annotation: VR
sequence annotation: Lambda Red reverse primer
sequence annotation: TonB terminator
sequence: https://synbiohub.org/public/igem/BBa_K864005_sequence/1

Lux terminator
type: SequenceAnnotation
persistent identity: annotation2200051
display id: annotation2200051
version: 1
top level: BBa_K864005
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K864005/annotation2200051/range2200051/1

https://synbiohub.org/public/igem/BBa_K864005/annotation2200051/range2200051/1
type: Range
persistent identity: range2200051
display id: range2200051
version: 1
direction: direction/forward
top level: BBa_K864005
start: 2454
end: 2499
orientation: inline

Lambda Red forward primer
type: SequenceAnnotation
persistent identity: annotation2200050
display id: annotation2200050
version: 1
top level: BBa_K864005
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K864005/annotation2200050/range2200050/1

https://synbiohub.org/public/igem/BBa_K864005/annotation2200050/range2200050/1
type: Range
persistent identity: range2200050
display id: range2200050
version: 1
direction: direction/forward
top level: BBa_K864005
start: 2433
end: 2450
orientation: inline

pSC101 ori BBa_K864050
type: SequenceAnnotation
persistent identity: annotation2200048
display id: annotation2200048
version: 1
top level: BBa_K864005
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864005/annotation2200048/range2200048/1

https://synbiohub.org/public/igem/BBa_K864005/annotation2200048/range2200048/1
type: Range
persistent identity: range2200048
display id: range2200048
version: 1
direction: direction/forward
top level: BBa_K864005
start: 190
end: 2405
orientation: inline

rrnC terminator
type: SequenceAnnotation
persistent identity: annotation2200047
display id: annotation2200047
version: 1
top level: BBa_K864005
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K864005/annotation2200047/range2200047/1

https://synbiohub.org/public/igem/BBa_K864005/annotation2200047/range2200047/1
type: Range
persistent identity: range2200047
display id: range2200047
version: 1
direction: direction/forward
top level: BBa_K864005
start: 136
end: 175
orientation: inline

VR
type: SequenceAnnotation
persistent identity: annotation2200046
display id: annotation2200046
version: 1
top level: BBa_K864005
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K864005/annotation2200046/range2200046/1

https://synbiohub.org/public/igem/BBa_K864005/annotation2200046/range2200046/1
type: Range
persistent identity: range2200046
display id: range2200046
version: 1
direction: direction/reverse
top level: BBa_K864005
start: 116
end: 135
orientation: inline

Lambda Red reverse primer
type: SequenceAnnotation
persistent identity: annotation2200041
display id: annotation2200041
version: 1
top level: BBa_K864005
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K864005/annotation2200041/range2200041/1

https://synbiohub.org/public/igem/BBa_K864005/annotation2200041/range2200041/1
type: Range
persistent identity: range2200041
display id: range2200041
version: 1
direction: direction/reverse
top level: BBa_K864005
start: 18
end: 38
orientation: inline

TonB terminator
type: SequenceAnnotation
persistent identity: annotation2200045
display id: annotation2200045
version: 1
top level: BBa_K864005
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K864005/annotation2200045/range2200045/1

https://synbiohub.org/public/igem/BBa_K864005/annotation2200045/range2200045/1
type: Range
persistent identity: range2200045
display id: range2200045
version: 1
direction: direction/forward
top level: BBa_K864005
start: 47
end: 115
orientation: inline

pSB8S15
type: ComponentDefinition
persistent identity: BBa K864019
display id: BBa_K864019
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Low copy BioBrick temperature sensitive standard vector
creator: Erik Lundin
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:37Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1124_
m user id: 0
owner id: 9827
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: pSB8S15 is a BioBrick standard vector with low copy pSC101ts replication origin (~5 copies per cell) and spectinomycin antibiotic resistance marker, especially usable for Lambda Red recombineering in E coli. The pSC101ts is a temperature sensitive pSC101 repelication origin and propagates at 30???C but stop replicating at 42???C. The backbone sequence is based on pSB3T5, but the E coli His operon terminator BBa_B0053 has been replaced with the late terminator of the Salmonella phage P22.
mutable notes: The resistance cassette is terminated by a Lux bidirectional terminator. Adjacent to this terminator there is a designed primer binding site to where the forward primer for lambda red can be designed to bind to no matter what the insert in the cloning site is or what the resistance is. The resistance cassette is flanked by SalI and SacI restriction sites for easy switching of resistance in the backbone. The pSC101ts origin of replication is flaked by NheI and MluI restriction sites for easy switching of origin of replication.
mutable provenance: Based on BBa_K864017
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence: https://synbiohub.org/public/igem/BBa_K864019_sequence/1

pSB4A5
type: ComponentDefinition
persistent identity: pSB4A5
display id: pSB4A5
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Low copy BioBrick standard vector
creator: Reshma Shetty
created: 2007-02-26T12:00:00Z
modified: 2015-05-08T01:14:55Z
discontinued: false
dominant: false
experience: experience/None
group u list: _41_
m user id: 0
owner id: 126
owning group id: 70
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: pSB4A5 is a low copy plasmid with ampicillin resistance. It has a repA pSC101-derived replication origin. (Copy number ~10-12, Lutz and Bujard, 1997.) It is derived from the BioBricks vector scaffold BBa_I51001.
mutable notes: See http://openwetware.org/wiki/Synthetic_Biology:Vectors/pSB%2A%2A5_design
mutable provenance: BBa_I51001, pSC101 origin and a new version of ampR stripped of restriction sites.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
component: BBa_G00001
component: BBa_G0002
component: BBa_P1002
component: BBa_G0003
component: BBa_I50042
component: BBa_B0055
component: BBa_G00100
component: BBa_B0062
component: BBa_B0054
component: BBa_B0042
component: BBa_G00102
component: BBa_B0043
component: BBa_G00000
component: BBa_B0044
component: BBa_B0053
component: BBa_G0004
sequence annotation: BBa_G00102
sequence annotation: BBa_G0004
sequence annotation: BBa_B0043
sequence annotation: BBa_B0054
sequence annotation: BBa_B0062
sequence annotation: BBa_I50042
sequence annotation: BBa_G0003
sequence annotation: BBa_P1002
sequence annotation: BBa_G00001
sequence annotation: BBa_B0055
sequence annotation: BBa_G00000
sequence annotation: BBa_G0002
sequence annotation: BBa_B0042
sequence annotation: BBa_B0044
sequence annotation: BBa_B0053
sequence annotation: BBa_G00100
sequence: https://synbiohub.org/public/igem/pSB4A5_sequence/1

BBa_G00001
type: Component
persistent identity: component2236317
display id: component2236317
version: 1
top level: pSB4A5
access: public
definition: https://synbiohub.org/public/igem/BBa_G00001/1

BBa_G0002
type: Component
persistent identity: component2236366
display id: component2236366
version: 1
top level: pSB4A5
access: public
definition: https://synbiohub.org/public/igem/BBa_G0002/1

BBa_P1002
type: Component
persistent identity: component2236371
display id: component2236371
version: 1
top level: pSB4A5
access: public
definition: https://synbiohub.org/public/igem/BBa_P1002/1

BBa_G0003
type: Component
persistent identity: component2236337
display id: component2236337
version: 1
top level: pSB4A5
access: public
definition: https://synbiohub.org/public/igem/BBa_G0003/1

BBa_I50042
type: Component
persistent identity: component2236365
display id: component2236365
version: 1
top level: pSB4A5
access: public
definition: https://synbiohub.org/public/igem/BBa_I50042/1

BBa_B0055
type: Component
persistent identity: component2236379
display id: component2236379
version: 1
top level: pSB4A5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0055/1

BBa_G00100
type: Component
persistent identity: component2236376
display id: component2236376
version: 1
top level: pSB4A5
access: public
definition: https://synbiohub.org/public/igem/BBa_G00100/1

BBa_B0062
type: Component
persistent identity: component2236336
display id: component2236336
version: 1
top level: pSB4A5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0062/1

BBa_B0054
type: Component
persistent identity: component2236332
display id: component2236332
version: 1
top level: pSB4A5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0054/1

BBa_B0042
type: Component
persistent identity: component2236329
display id: component2236329
version: 1
top level: pSB4A5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0042/1

BBa_G00102
type: Component
persistent identity: component2236334
display id: component2236334
version: 1
top level: pSB4A5
access: public
definition: https://synbiohub.org/public/igem/BBa_G00102/1

BBa_B0043
type: Component
persistent identity: component2236391
display id: component2236391
version: 1
top level: pSB4A5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0043/1

BBa_G00000
type: Component
persistent identity: component2236396
display id: component2236396
version: 1
top level: pSB4A5
access: public
definition: https://synbiohub.org/public/igem/BBa_G00000/1

BBa_B0044
type: Component
persistent identity: component2236322
display id: component2236322
version: 1
top level: pSB4A5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0044/1

BBa_B0042
type: Component
persistent identity: component2236386
display id: component2236386
version: 1
top level: pSB4A5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0042/1

BBa_B0053
type: Component
persistent identity: component2236374
display id: component2236374
version: 1
top level: pSB4A5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0053/1

BBa_G0004
type: Component
persistent identity: component2236372
display id: component2236372
version: 1
top level: pSB4A5
access: public
definition: https://synbiohub.org/public/igem/BBa_G0004/1

BBa_G00102
type: SequenceAnnotation
persistent identity: annotation2236334
display id: annotation2236334
version: 1
top level: pSB4A5
location: https://synbiohub.org/public/igem/pSB4A5/annotation2236334/range2236334/1
component: BBa_G00102

https://synbiohub.org/public/igem/pSB4A5/annotation2236334/range2236334/1
type: Range
persistent identity: range2236334
display id: range2236334
version: 1
direction: direction/reverse
top level: pSB4A5
start: 116
end: 135
orientation: inline

BBa_G0004
type: SequenceAnnotation
persistent identity: annotation2236372
display id: annotation2236372
version: 1
top level: pSB4A5
location: https://synbiohub.org/public/igem/pSB4A5/annotation2236372/range2236372/1
component: BBa_G0004

https://synbiohub.org/public/igem/pSB4A5/annotation2236372/range2236372/1
type: Range
persistent identity: range2236372
display id: range2236372
version: 1
direction: direction/reverse
top level: pSB4A5
start: 3172
end: 3178
orientation: inline

BBa_B0043
type: SequenceAnnotation
persistent identity: annotation2236391
display id: annotation2236391
version: 1
top level: pSB4A5
location: https://synbiohub.org/public/igem/pSB4A5/annotation2236391/range2236391/1
component: BBa_B0043

https://synbiohub.org/public/igem/pSB4A5/annotation2236391/range2236391/1
type: Range
persistent identity: range2236391
display id: range2236391
version: 1
direction: direction/reverse
top level: pSB4A5
start: 3361
end: 3373
orientation: inline

BBa_B0054
type: SequenceAnnotation
persistent identity: annotation2236332
display id: annotation2236332
version: 1
top level: pSB4A5
location: https://synbiohub.org/public/igem/pSB4A5/annotation2236332/range2236332/1
component: BBa_B0054

https://synbiohub.org/public/igem/pSB4A5/annotation2236332/range2236332/1
type: Range
persistent identity: range2236332
display id: range2236332
version: 1
direction: direction/reverse
top level: pSB4A5
start: 47
end: 115
orientation: inline

BBa_B0062
type: SequenceAnnotation
persistent identity: annotation2236336
display id: annotation2236336
version: 1
top level: pSB4A5
location: https://synbiohub.org/public/igem/pSB4A5/annotation2236336/range2236336/1
component: BBa_B0062

https://synbiohub.org/public/igem/pSB4A5/annotation2236336/range2236336/1
type: Range
persistent identity: range2236336
display id: range2236336
version: 1
direction: direction/reverse
top level: pSB4A5
start: 136
end: 176
orientation: inline

BBa_I50042
type: SequenceAnnotation
persistent identity: annotation2236365
display id: annotation2236365
version: 1
top level: pSB4A5
location: https://synbiohub.org/public/igem/pSB4A5/annotation2236365/range2236365/1
component: BBa_I50042

https://synbiohub.org/public/igem/pSB4A5/annotation2236365/range2236365/1
type: Range
persistent identity: range2236365
display id: range2236365
version: 1
direction: direction/reverse
top level: pSB4A5
start: 184
end: 2220
orientation: inline

BBa_G0003
type: SequenceAnnotation
persistent identity: annotation2236337
display id: annotation2236337
version: 1
top level: pSB4A5
location: https://synbiohub.org/public/igem/pSB4A5/annotation2236337/range2236337/1
component: BBa_G0003

https://synbiohub.org/public/igem/pSB4A5/annotation2236337/range2236337/1
type: Range
persistent identity: range2236337
display id: range2236337
version: 1
direction: direction/reverse
top level: pSB4A5
start: 177
end: 183
orientation: inline

BBa_P1002
type: SequenceAnnotation
persistent identity: annotation2236371
display id: annotation2236371
version: 1
top level: pSB4A5
location: https://synbiohub.org/public/igem/pSB4A5/annotation2236371/range2236371/1
component: BBa_P1002

https://synbiohub.org/public/igem/pSB4A5/annotation2236371/range2236371/1
type: Range
persistent identity: range2236371
display id: range2236371
version: 1
direction: direction/reverse
top level: pSB4A5
start: 2229
end: 3171
orientation: inline

BBa_G00001
type: SequenceAnnotation
persistent identity: annotation2236317
display id: annotation2236317
version: 1
top level: pSB4A5
location: https://synbiohub.org/public/igem/pSB4A5/annotation2236317/range2236317/1
component: BBa_G00001

https://synbiohub.org/public/igem/pSB4A5/annotation2236317/range2236317/1
type: Range
persistent identity: range2236317
display id: range2236317
version: 1
direction: direction/reverse
top level: pSB4A5
start: 1
end: 21
orientation: inline

BBa_B0055
type: SequenceAnnotation
persistent identity: annotation2236379
display id: annotation2236379
version: 1
top level: pSB4A5
location: https://synbiohub.org/public/igem/pSB4A5/annotation2236379/range2236379/1
component: BBa_B0055

https://synbiohub.org/public/igem/pSB4A5/annotation2236379/range2236379/1
type: Range
persistent identity: range2236379
display id: range2236379
version: 1
direction: direction/reverse
top level: pSB4A5
start: 3271
end: 3348
orientation: inline

BBa_G00000
type: SequenceAnnotation
persistent identity: annotation2236396
display id: annotation2236396
version: 1
top level: pSB4A5
location: https://synbiohub.org/public/igem/pSB4A5/annotation2236396/range2236396/1
component: BBa_G00000

https://synbiohub.org/public/igem/pSB4A5/annotation2236396/range2236396/1
type: Range
persistent identity: range2236396
display id: range2236396
version: 1
direction: direction/reverse
top level: pSB4A5
start: 3374
end: 3395
orientation: inline

BBa_G0002
type: SequenceAnnotation
persistent identity: annotation2236366
display id: annotation2236366
version: 1
top level: pSB4A5
location: https://synbiohub.org/public/igem/pSB4A5/annotation2236366/range2236366/1
component: BBa_G0002

https://synbiohub.org/public/igem/pSB4A5/annotation2236366/range2236366/1
type: Range
persistent identity: range2236366
display id: range2236366
version: 1
direction: direction/reverse
top level: pSB4A5
start: 2221
end: 2228
orientation: inline

BBa_B0042
type: SequenceAnnotation
persistent identity: annotation2236386
display id: annotation2236386
version: 1
top level: pSB4A5
location: https://synbiohub.org/public/igem/pSB4A5/annotation2236386/range2236386/1
component: BBa_B0042

https://synbiohub.org/public/igem/pSB4A5/annotation2236386/range2236386/1
type: Range
persistent identity: range2236386
display id: range2236386
version: 1
direction: direction/reverse
top level: pSB4A5
start: 3349
end: 3360
orientation: inline

BBa_B0044
type: SequenceAnnotation
persistent identity: annotation2236322
display id: annotation2236322
version: 1
top level: pSB4A5
location: https://synbiohub.org/public/igem/pSB4A5/annotation2236322/range2236322/1
component: BBa_B0044

https://synbiohub.org/public/igem/pSB4A5/annotation2236322/range2236322/1
type: Range
persistent identity: range2236322
display id: range2236322
version: 1
direction: direction/reverse
top level: pSB4A5
start: 22
end: 34
orientation: inline

BBa_B0042
type: SequenceAnnotation
persistent identity: annotation2236329
display id: annotation2236329
version: 1
top level: pSB4A5
location: https://synbiohub.org/public/igem/pSB4A5/annotation2236329/range2236329/1
component: BBa_B0042

https://synbiohub.org/public/igem/pSB4A5/annotation2236329/range2236329/1
type: Range
persistent identity: range2236329
display id: range2236329
version: 1
direction: direction/reverse
top level: pSB4A5
start: 35
end: 46
orientation: inline

BBa_B0053
type: SequenceAnnotation
persistent identity: annotation2236374
display id: annotation2236374
version: 1
top level: pSB4A5
location: https://synbiohub.org/public/igem/pSB4A5/annotation2236374/range2236374/1
component: BBa_B0053

https://synbiohub.org/public/igem/pSB4A5/annotation2236374/range2236374/1
type: Range
persistent identity: range2236374
display id: range2236374
version: 1
direction: direction/reverse
top level: pSB4A5
start: 3179
end: 3250
orientation: inline

BBa_G00100
type: SequenceAnnotation
persistent identity: annotation2236376
display id: annotation2236376
version: 1
top level: pSB4A5
location: https://synbiohub.org/public/igem/pSB4A5/annotation2236376/range2236376/1
component: BBa_G00100

https://synbiohub.org/public/igem/pSB4A5/annotation2236376/range2236376/1
type: Range
persistent identity: range2236376
display id: range2236376
version: 1
direction: direction/reverse
top level: pSB4A5
start: 3251
end: 3270
orientation: inline

BBa_K864010
type: ComponentDefinition
persistent identity: BBa K864010
display id: BBa_K864010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Low copy BioBrick standard vector with LacI
creator: Erik Lundin
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:37Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1124_
m user id: 0
owner id: 9827
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: pSB4K15Iq is a BioBrick standard vector with low copy pSC101 replication origin (~5 copies per cell) and kanamycin antibiotic resistance marker, especially usable for Lambda Red recombineering in E coli. The backbone sequence is based on pSB3T5, but the E coli His operon terminator BBa_B0053 has been replaced with the late terminator of the Salmonella phage P22. This vector carries a LacIq cassette on the backbone for tight repression of any lac promoter cloned into the cloning site.
mutable notes: The resistance cassette is terminated by a Lux bidirectional terminator. Adjacent to this terminator there is a designed primer binding site to where the forward primer for lambda red can be designed to bind to no matter what the insert in the cloning site is or what the resistance is. The resistance cassette is flanked by SalI and SacI restriction sites for easy switching of resistance in the backbone.
mutable provenance: Based on BBa_K864002
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence: https://synbiohub.org/public/igem/BBa_K864010_sequence/1

BBa_K125000
type: ComponentDefinition
persistent identity: BBa K125000
display id: BBa_K125000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Broad host range BioBrick plasmid derived from pRL1383a with low to medium copy number
creator: Grace Kwan
created: 2008-07-23T11:00:00Z
modified: 2015-05-08T01:09:44Z
discontinued: false
dominant: false
experience: experience/None
group u list: _179_
m user id: 0
owner id: 3229
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: RSF1010 is a broad-host-range plasmid first described in 1974 (Guerry 1974), with its entire sequence and gene organization subsequently described in 1989 (Scholz 1989). It is a naturally occurring 8.6kb broad-host-range plasmid in the E. coli incompatibility group Q. The conjugative transfer and stable replication of this plasmid are possible due to the mob genes with the associated origin of transfer (oriT) and the rep genes with the associated origin of vegetative replication (oriV), respectively. RSF1010 derived plasmids which include the oriV and associated Rep proteins are stably maintained in Pseudomonas (Bagdasarian 1981), Caulobacter (Umelo-Njaka et al. 2001), Erwinia, and Serratia (Leemans 1987). In addition, RSF1010 derived plasmids including the oriV, oriT and its associated rep and mob genes are transferred by conjugation to at least four cyanobacteria strains (Mermet-Bouvier 1993). These cyanobacteria strains include Synechocystis PCC6803 and PCC6714 and Synechococcus PCC7942 and PCC6301. A number of RSF1010 derived plasmids have been constructed due to the utility of this broad-host-range plasmid. For example, pSB2A, containing a 5.6kb RSF1010 derived region including the necessary mobilization and replication regions, can be transferred through conjugation to and stably maintained in Synechocystis PCC6803, PCC6714, and Synechococcus PCC7942 and PCC6301 (Marraccini 1993). The RSF1010 plasmid used in this study, pRL1383a was constructed for use in genomic studies of the diazotrophic, multicellular cyanobacterium Anabaena PCC 7120. This plasmid contains the mob and rep regions necessary for conjugation and autonomous replication, respectively. Additionally this vector is made resistant to Streptomycin and Spectinomycin due to the presence of the aadA gene (Wolk 2007). This plasmid can be mobilized by the E. coli derived self-transmissible plasmid RP4. Mobilization genes are not necessary for transfer of a mobilizable plasmid if the self-transmissible pl…
mutable notes: The original pRL1383a MCS has been replaced by the BioBrick BBa_B0034 and its flanking BioBrick restriction sites.
mutable provenance: Plasmid is derived from pRL1383a.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: oriT
sequence annotation: BioBrick suffix
sequence annotation: P5 promoter
sequence annotation: T4 32 terminator
sequence annotation: T7 promoter
sequence annotation: omega interposon
sequence annotation: BioBrick prefix
sequence annotation: oriR
sequence annotation: spectinomycin marker
sequence annotation: VF2
sequence annotation: cosN
sequence annotation: loxP
sequence annotation: VR
sequence annotation: RSF1010 derived region
sequence: https://synbiohub.org/public/igem/BBa_K125000_sequence/1

oriT
type: SequenceAnnotation
persistent identity: annotation1969230
display id: annotation1969230
version: 1
top level: BBa_K125000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K125000/annotation1969230/range1969230/1

https://synbiohub.org/public/igem/BBa_K125000/annotation1969230/range1969230/1
type: Range
persistent identity: range1969230
display id: range1969230
version: 1
direction: direction/forward
top level: BBa_K125000
start: 3476
end: 3633
orientation: inline

BioBrick suffix
type: SequenceAnnotation
persistent identity: annotation1999638
display id: annotation1999638
version: 1
top level: BBa_K125000
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K125000/annotation1999638/range1999638/1

https://synbiohub.org/public/igem/BBa_K125000/annotation1999638/range1999638/1
type: Range
persistent identity: range1999638
display id: range1999638
version: 1
direction: direction/forward
top level: BBa_K125000
start: 1
end: 21
orientation: inline

P5 promoter
type: SequenceAnnotation
persistent identity: annotation1969227
display id: annotation1969227
version: 1
top level: BBa_K125000
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K125000/annotation1969227/range1969227/1

https://synbiohub.org/public/igem/BBa_K125000/annotation1969227/range1969227/1
type: Range
persistent identity: range1969227
display id: range1969227
version: 1
direction: direction/forward
top level: BBa_K125000
start: 8200
end: 8230
orientation: inline

T4 32 terminator
type: SequenceAnnotation
persistent identity: annotation1999636
display id: annotation1999636
version: 1
top level: BBa_K125000
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K125000/annotation1999636/range1999636/1

https://synbiohub.org/public/igem/BBa_K125000/annotation1999636/range1999636/1
type: Range
persistent identity: range1999636
display id: range1999636
version: 1
direction: direction/forward
top level: BBa_K125000
start: 343
end: 374
orientation: inline

T4 32 terminator
type: SequenceAnnotation
persistent identity: annotation1999637
display id: annotation1999637
version: 1
top level: BBa_K125000
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K125000/annotation1999637/range1999637/1

https://synbiohub.org/public/igem/BBa_K125000/annotation1999637/range1999637/1
type: Range
persistent identity: range1999637
display id: range1999637
version: 1
direction: direction/forward
top level: BBa_K125000
start: 2249
end: 2280
orientation: inline

T7 promoter
type: SequenceAnnotation
persistent identity: annotation1999633
display id: annotation1999633
version: 1
top level: BBa_K125000
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K125000/annotation1999633/range1999633/1

https://synbiohub.org/public/igem/BBa_K125000/annotation1999633/range1999633/1
type: Range
persistent identity: range1999633
display id: range1999633
version: 1
direction: direction/forward
top level: BBa_K125000
start: 8880
end: 8898
orientation: inline

omega interposon
type: SequenceAnnotation
persistent identity: annotation1969583
display id: annotation1969583
version: 1
top level: BBa_K125000
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K125000/annotation1969583/range1969583/1

https://synbiohub.org/public/igem/BBa_K125000/annotation1969583/range1969583/1
type: Range
persistent identity: range1969583
display id: range1969583
version: 1
direction: direction/forward
top level: BBa_K125000
start: 463
end: 1255
orientation: inline

BioBrick prefix
type: SequenceAnnotation
persistent identity: annotation1999639
display id: annotation1999639
version: 1
top level: BBa_K125000
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K125000/annotation1999639/range1999639/1

https://synbiohub.org/public/igem/BBa_K125000/annotation1999639/range1999639/1
type: Range
persistent identity: range1999639
display id: range1999639
version: 1
direction: direction/forward
top level: BBa_K125000
start: 9026
end: 9047
orientation: inline

oriR
type: SequenceAnnotation
persistent identity: annotation1969581
display id: annotation1969581
version: 1
top level: BBa_K125000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K125000/annotation1969581/range1969581/1

https://synbiohub.org/public/igem/BBa_K125000/annotation1969581/range1969581/1
type: Range
persistent identity: range1969581
display id: range1969581
version: 1
direction: direction/forward
top level: BBa_K125000
start: 2819
end: 3243
orientation: inline

spectinomycin marker
type: SequenceAnnotation
persistent identity: annotation1996995
display id: annotation1996995
version: 1
top level: BBa_K125000
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K125000/annotation1996995/range1996995/1

https://synbiohub.org/public/igem/BBa_K125000/annotation1996995/range1996995/1
type: Range
persistent identity: range1996995
display id: range1996995
version: 1
direction: direction/forward
top level: BBa_K125000
start: 933
end: 1943
orientation: inline

VF2
type: SequenceAnnotation
persistent identity: annotation1969229
display id: annotation1969229
version: 1
top level: BBa_K125000
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K125000/annotation1969229/range1969229/1

https://synbiohub.org/public/igem/BBa_K125000/annotation1969229/range1969229/1
type: Range
persistent identity: range1969229
display id: range1969229
version: 1
direction: direction/forward
top level: BBa_K125000
start: 8908
end: 8927
orientation: inline

cosN
type: SequenceAnnotation
persistent identity: annotation1999634
display id: annotation1999634
version: 1
top level: BBa_K125000
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K125000/annotation1999634/range1999634/1

https://synbiohub.org/public/igem/BBa_K125000/annotation1999634/range1999634/1
type: Range
persistent identity: range1999634
display id: range1999634
version: 1
direction: direction/forward
top level: BBa_K125000
start: 8616
end: 8705
orientation: inline

loxP
type: SequenceAnnotation
persistent identity: annotation1999635
display id: annotation1999635
version: 1
top level: BBa_K125000
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K125000/annotation1999635/range1999635/1

https://synbiohub.org/public/igem/BBa_K125000/annotation1999635/range1999635/1
type: Range
persistent identity: range1999635
display id: range1999635
version: 1
direction: direction/forward
top level: BBa_K125000
start: 8831
end: 8864
orientation: inline

VR
type: SequenceAnnotation
persistent identity: annotation1969228
display id: annotation1969228
version: 1
top level: BBa_K125000
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K125000/annotation1969228/range1969228/1

https://synbiohub.org/public/igem/BBa_K125000/annotation1969228/range1969228/1
type: Range
persistent identity: range1969228
display id: range1969228
version: 1
direction: direction/reverse
top level: BBa_K125000
start: 78
end: 97
orientation: inline

RSF1010 derived region
type: SequenceAnnotation
persistent identity: annotation1969582
display id: annotation1969582
version: 1
top level: BBa_K125000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K125000/annotation1969582/range1969582/1

https://synbiohub.org/public/igem/BBa_K125000/annotation1969582/range1969582/1
type: Range
persistent identity: range1969582
display id: range1969582
version: 1
direction: direction/forward
top level: BBa_K125000
start: 2664
end: 8356
orientation: inline

BBa_K864004
type: ComponentDefinition
persistent identity: BBa K864004
display id: BBa_K864004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Low copy BioBrick standard vector
creator: Erik Lundin
created: 2012-09-22T11:00:00Z
modified: 2015-05-08T01:13:37Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1124_
m user id: 0
owner id: 9827
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: pSB4A15 is a BioBrick standard vector with low copy pSC101 replication origin (~5 copies per cell) and ampicillin antibiotic resistance marker, especially usable for Lambda Red recombineering in E coli. The backbone sequence is based on pSB3T5, but the E coli His operon terminator BBa_B0053 has been replaced with the late terminator of the Salmonella phage P22.
mutable notes: The resistance cassette is terminated by a Lux bidirectional terminator. Adjacent to this terminator there is a designed primer binding site to where the forward primer for lambda red can be designed to bind to no matter what the insert in the cloning site is or what the resistance is. The resistance cassette is flanked by SalI and SacI restriction sites for easy switching of resistance in the backbone. The pSC101 origin of replication is flaked by NheI and MluI restriction sites for easy switching of origin of replication.
mutable provenance: based on pSB3T5
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: rrnC terminator
sequence annotation: VF2
sequence annotation: T3 terminator
sequence annotation: -10
sequence annotation: Lambda Red forward primer
sequence annotation: pSC101 ori BBa_K864050
sequence annotation: -35
sequence annotation: FRT site
sequence annotation: RBS
sequence annotation: Lambda Red reverse primer
sequence annotation: VR
sequence annotation: Lux terminator
sequence annotation: P22 late terminator
sequence annotation: AmpR P1002
sequence annotation: TonB terminator
sequence: https://synbiohub.org/public/igem/BBa_K864004_sequence/1

rrnC terminator
type: SequenceAnnotation
persistent identity: annotation2198868
display id: annotation2198868
version: 1
top level: BBa_K864004
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K864004/annotation2198868/range2198868/1

https://synbiohub.org/public/igem/BBa_K864004/annotation2198868/range2198868/1
type: Range
persistent identity: range2198868
display id: range2198868
version: 1
direction: direction/forward
top level: BBa_K864004
start: 136
end: 175
orientation: inline

VF2
type: SequenceAnnotation
persistent identity: annotation2198873
display id: annotation2198873
version: 1
top level: BBa_K864004
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K864004/annotation2198873/range2198873/1

https://synbiohub.org/public/igem/BBa_K864004/annotation2198873/range2198873/1
type: Range
persistent identity: range2198873
display id: range2198873
version: 1
direction: direction/forward
top level: BBa_K864004
start: 3564
end: 3583
orientation: inline

T3 terminator
type: SequenceAnnotation
persistent identity: annotation2198874
display id: annotation2198874
version: 1
top level: BBa_K864004
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K864004/annotation2198874/range2198874/1

https://synbiohub.org/public/igem/BBa_K864004/annotation2198874/range2198874/1
type: Range
persistent identity: range2198874
display id: range2198874
version: 1
direction: direction/forward
top level: BBa_K864004
start: 3584
end: 3661
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2198877
display id: annotation2198877
version: 1
top level: BBa_K864004
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K864004/annotation2198877/range2198877/1

https://synbiohub.org/public/igem/BBa_K864004/annotation2198877/range2198877/1
type: Range
persistent identity: range2198877
display id: range2198877
version: 1
direction: direction/reverse
top level: BBa_K864004
start: 3421
end: 3426
orientation: inline

Lambda Red forward primer
type: SequenceAnnotation
persistent identity: annotation2198870
display id: annotation2198870
version: 1
top level: BBa_K864004
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K864004/annotation2198870/range2198870/1

https://synbiohub.org/public/igem/BBa_K864004/annotation2198870/range2198870/1
type: Range
persistent identity: range2198870
display id: range2198870
version: 1
direction: direction/forward
top level: BBa_K864004
start: 2433
end: 2450
orientation: inline

pSC101 ori BBa_K864050
type: SequenceAnnotation
persistent identity: annotation2198869
display id: annotation2198869
version: 1
top level: BBa_K864004
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864004/annotation2198869/range2198869/1

https://synbiohub.org/public/igem/BBa_K864004/annotation2198869/range2198869/1
type: Range
persistent identity: range2198869
display id: range2198869
version: 1
direction: direction/forward
top level: BBa_K864004
start: 190
end: 2405
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2198878
display id: annotation2198878
version: 1
top level: BBa_K864004
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K864004/annotation2198878/range2198878/1

https://synbiohub.org/public/igem/BBa_K864004/annotation2198878/range2198878/1
type: Range
persistent identity: range2198878
display id: range2198878
version: 1
direction: direction/forward
top level: BBa_K864004
start: 3447
end: 3452
orientation: inline

FRT site
type: SequenceAnnotation
persistent identity: annotation2198879
display id: annotation2198879
version: 1
top level: BBa_K864004
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864004/annotation2198879/range2198879/1

https://synbiohub.org/public/igem/BBa_K864004/annotation2198879/range2198879/1
type: Range
persistent identity: range2198879
display id: range2198879
version: 1
direction: direction/reverse
top level: BBa_K864004
start: 2506
end: 2539
orientation: inline

FRT site
type: SequenceAnnotation
persistent identity: annotation2198880
display id: annotation2198880
version: 1
top level: BBa_K864004
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864004/annotation2198880/range2198880/1

https://synbiohub.org/public/igem/BBa_K864004/annotation2198880/range2198880/1
type: Range
persistent identity: range2198880
display id: range2198880
version: 1
direction: direction/reverse
top level: BBa_K864004
start: 3483
end: 3516
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2198876
display id: annotation2198876
version: 1
top level: BBa_K864004
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K864004/annotation2198876/range2198876/1

https://synbiohub.org/public/igem/BBa_K864004/annotation2198876/range2198876/1
type: Range
persistent identity: range2198876
display id: range2198876
version: 1
direction: direction/reverse
top level: BBa_K864004
start: 3408
end: 3412
orientation: inline

Lambda Red reverse primer
type: SequenceAnnotation
persistent identity: annotation2198865
display id: annotation2198865
version: 1
top level: BBa_K864004
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K864004/annotation2198865/range2198865/1

https://synbiohub.org/public/igem/BBa_K864004/annotation2198865/range2198865/1
type: Range
persistent identity: range2198865
display id: range2198865
version: 1
direction: direction/reverse
top level: BBa_K864004
start: 18
end: 38
orientation: inline

VR
type: SequenceAnnotation
persistent identity: annotation2198867
display id: annotation2198867
version: 1
top level: BBa_K864004
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K864004/annotation2198867/range2198867/1

https://synbiohub.org/public/igem/BBa_K864004/annotation2198867/range2198867/1
type: Range
persistent identity: range2198867
display id: range2198867
version: 1
direction: direction/forward
top level: BBa_K864004
start: 116
end: 135
orientation: inline

Lux terminator
type: SequenceAnnotation
persistent identity: annotation2198871
display id: annotation2198871
version: 1
top level: BBa_K864004
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K864004/annotation2198871/range2198871/1

https://synbiohub.org/public/igem/BBa_K864004/annotation2198871/range2198871/1
type: Range
persistent identity: range2198871
display id: range2198871
version: 1
direction: direction/forward
top level: BBa_K864004
start: 2454
end: 2499
orientation: inline

P22 late terminator
type: SequenceAnnotation
persistent identity: annotation2198872
display id: annotation2198872
version: 1
top level: BBa_K864004
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K864004/annotation2198872/range2198872/1

https://synbiohub.org/public/igem/BBa_K864004/annotation2198872/range2198872/1
type: Range
persistent identity: range2198872
display id: range2198872
version: 1
direction: direction/forward
top level: BBa_K864004
start: 3523
end: 3563
orientation: inline

AmpR P1002
type: SequenceAnnotation
persistent identity: annotation2198875
display id: annotation2198875
version: 1
top level: BBa_K864004
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K864004/annotation2198875/range2198875/1

https://synbiohub.org/public/igem/BBa_K864004/annotation2198875/range2198875/1
type: Range
persistent identity: range2198875
display id: range2198875
version: 1
direction: direction/reverse
top level: BBa_K864004
start: 2543
end: 3403
orientation: inline

TonB terminator
type: SequenceAnnotation
persistent identity: annotation2198866
display id: annotation2198866
version: 1
top level: BBa_K864004
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K864004/annotation2198866/range2198866/1

https://synbiohub.org/public/igem/BBa_K864004/annotation2198866/range2198866/1
type: Range
persistent identity: range2198866
display id: range2198866
version: 1
direction: direction/forward
top level: BBa_K864004
start: 47
end: 115
orientation: inline

Cyc1P Acce
type: ComponentDefinition
persistent identity: BBa K106014
display id: BBa_K106014
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: AarI AD acceptor vector (pRS315, Cyc1P, Adh1t)
creator: Sergio Peisajovich
created: 2008-10-23T11:00:00Z
modified: 2015-05-08T01:08:58Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _226_
m user id: 0
owner id: 2659
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: Acceptor vector for AarI based cloning. Accepts any combination of parts beginning with an A site and ending with a D site. Expression of construct is driven by a Cyc1 promoter, with Adh1 terminator.
mutable notes: The pRS315 vector has been modified with an AarI cloning cassette inserted between the PspOMI and SacI sites. Please note, the actual orientation of this vector is reversed.
mutable provenance: A modified pRS315 plasmid--CEN ARS yeast expression vector (LEU2 marker).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: A overhang
sequence annotation: f1 origin
sequence annotation: lacZalpha reporter
sequence annotation: Cyc1 Promoter
sequence annotation: T3 promoter
sequence annotation: LEU2 marker
sequence annotation: Stop codons in all 3 frames
sequence annotation: ampicillin resistance marker
sequence annotation: ampicillin resistance promoter
sequence annotation: T7 promoter
sequence annotation: D overhang
sequence annotation: lac promoter
sequence annotation: Start codon
sequence annotation: pBR322 origin
sequence annotation: Adh1 terminator
sequence annotation: CEN ARS
sequence: https://synbiohub.org/public/igem/BBa_K106014_sequence/1

A overhang
type: SequenceAnnotation
persistent identity: annotation1999685
display id: annotation1999685
version: 1
top level: Cyc1P Acce
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K106014/annotation1999685/range1999685/1

https://synbiohub.org/public/igem/BBa_K106014/annotation1999685/range1999685/1
type: Range
persistent identity: range1999685
display id: range1999685
version: 1
direction: direction/forward
top level: Cyc1P Acce
start: 6394
end: 6397
orientation: inline

f1 origin
type: SequenceAnnotation
persistent identity: annotation1999688
display id: annotation1999688
version: 1
top level: Cyc1P Acce
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K106014/annotation1999688/range1999688/1

https://synbiohub.org/public/igem/BBa_K106014/annotation1999688/range1999688/1
type: Range
persistent identity: range1999688
display id: range1999688
version: 1
direction: direction/forward
top level: Cyc1P Acce
start: 427
end: 733
orientation: inline

lacZalpha reporter
type: SequenceAnnotation
persistent identity: annotation1999687
display id: annotation1999687
version: 1
top level: Cyc1P Acce
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K106014/annotation1999687/range1999687/1

https://synbiohub.org/public/igem/BBa_K106014/annotation1999687/range1999687/1
type: Range
persistent identity: range1999687
display id: range1999687
version: 1
direction: direction/forward
top level: Cyc1P Acce
start: 246
end: 405
orientation: inline

Cyc1 Promoter
type: SequenceAnnotation
persistent identity: annotation1986801
display id: annotation1986801
version: 1
top level: Cyc1P Acce
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K106014/annotation1986801/range1986801/1

https://synbiohub.org/public/igem/BBa_K106014/annotation1986801/range1986801/1
type: Range
persistent identity: range1986801
display id: range1986801
version: 1
direction: direction/forward
top level: Cyc1P Acce
start: 6132
end: 6394
orientation: inline

T3 promoter
type: SequenceAnnotation
persistent identity: annotation1999692
display id: annotation1999692
version: 1
top level: Cyc1P Acce
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K106014/annotation1999692/range1999692/1

https://synbiohub.org/public/igem/BBa_K106014/annotation1999692/range1999692/1
type: Range
persistent identity: range1999692
display id: range1999692
version: 1
direction: direction/forward
top level: Cyc1P Acce
start: 6095
end: 6114
orientation: inline

LEU2 marker
type: SequenceAnnotation
persistent identity: annotation1986804
display id: annotation1986804
version: 1
top level: Cyc1P Acce
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K106014/annotation1986804/range1986804/1

https://synbiohub.org/public/igem/BBa_K106014/annotation1986804/range1986804/1
type: Range
persistent identity: range1986804
display id: range1986804
version: 1
direction: direction/forward
top level: Cyc1P Acce
start: 1570
end: 2676
orientation: inline

Stop codons in all 3 frames
type: SequenceAnnotation
persistent identity: annotation1999703
display id: annotation1999703
version: 1
top level: Cyc1P Acce
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K106014/annotation1999703/range1999703/1

https://synbiohub.org/public/igem/BBa_K106014/annotation1999703/range1999703/1
type: Range
persistent identity: range1999703
display id: range1999703
version: 1
direction: direction/forward
top level: Cyc1P Acce
start: 11
end: 21
orientation: inline

ampicillin resistance marker
type: SequenceAnnotation
persistent identity: annotation1986800
display id: annotation1986800
version: 1
top level: Cyc1P Acce
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K106014/annotation1986800/range1986800/1

https://synbiohub.org/public/igem/BBa_K106014/annotation1986800/range1986800/1
type: Range
persistent identity: range1986800
display id: range1986800
version: 1
direction: direction/forward
top level: Cyc1P Acce
start: 4053
end: 4911
orientation: inline

ampicillin resistance promoter
type: SequenceAnnotation
persistent identity: annotation1999689
display id: annotation1999689
version: 1
top level: Cyc1P Acce
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K106014/annotation1999689/range1999689/1

https://synbiohub.org/public/igem/BBa_K106014/annotation1999689/range1999689/1
type: Range
persistent identity: range1999689
display id: range1999689
version: 1
direction: direction/forward
top level: Cyc1P Acce
start: 3983
end: 4011
orientation: inline

T7 promoter
type: SequenceAnnotation
persistent identity: annotation1999686
display id: annotation1999686
version: 1
top level: Cyc1P Acce
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K106014/annotation1999686/range1999686/1

https://synbiohub.org/public/igem/BBa_K106014/annotation1999686/range1999686/1
type: Range
persistent identity: range1999686
display id: range1999686
version: 1
direction: direction/reverse
top level: Cyc1P Acce
start: 224
end: 242
orientation: inline

D overhang
type: SequenceAnnotation
persistent identity: annotation1986802
display id: annotation1986802
version: 1
top level: Cyc1P Acce
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K106014/annotation1986802/range1986802/1

https://synbiohub.org/public/igem/BBa_K106014/annotation1986802/range1986802/1
type: Range
persistent identity: range1986802
display id: range1986802
version: 1
direction: direction/forward
top level: Cyc1P Acce
start: 1
end: 4
orientation: inline

lac promoter
type: SequenceAnnotation
persistent identity: annotation1999691
display id: annotation1999691
version: 1
top level: Cyc1P Acce
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K106014/annotation1999691/range1999691/1

https://synbiohub.org/public/igem/BBa_K106014/annotation1999691/range1999691/1
type: Range
persistent identity: range1999691
display id: range1999691
version: 1
direction: direction/forward
top level: Cyc1P Acce
start: 5996
end: 6025
orientation: inline

Start codon
type: SequenceAnnotation
persistent identity: annotation1999704
display id: annotation1999704
version: 1
top level: Cyc1P Acce
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K106014/annotation1999704/range1999704/1

https://synbiohub.org/public/igem/BBa_K106014/annotation1999704/range1999704/1
type: Range
persistent identity: range1999704
display id: range1999704
version: 1
direction: direction/forward
top level: Cyc1P Acce
start: 6385
end: 6387
orientation: inline

pBR322 origin
type: SequenceAnnotation
persistent identity: annotation1999690
display id: annotation1999690
version: 1
top level: Cyc1P Acce
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K106014/annotation1999690/range1999690/1

https://synbiohub.org/public/igem/BBa_K106014/annotation1999690/range1999690/1
type: Range
persistent identity: range1999690
display id: range1999690
version: 1
direction: direction/forward
top level: Cyc1P Acce
start: 5068
end: 5687
orientation: inline

Adh1 terminator
type: SequenceAnnotation
persistent identity: annotation1986803
display id: annotation1986803
version: 1
top level: Cyc1P Acce
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K106014/annotation1986803/range1986803/1

https://synbiohub.org/public/igem/BBa_K106014/annotation1986803/range1986803/1
type: Range
persistent identity: range1986803
display id: range1986803
version: 1
direction: direction/forward
top level: Cyc1P Acce
start: 23
end: 216
orientation: inline

CEN ARS
type: SequenceAnnotation
persistent identity: annotation1986799
display id: annotation1986799
version: 1
top level: Cyc1P Acce
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K106014/annotation1986799/range1986799/1

https://synbiohub.org/public/igem/BBa_K106014/annotation1986799/range1986799/1
type: Range
persistent identity: range1986799
display id: range1986799
version: 1
direction: direction/reverse
top level: Cyc1P Acce
start: 3403
end: 3921
orientation: inline

Eukaryotic
type: ComponentDefinition
persistent identity: BBa K157040
display id: BBa_K157040
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Eukaryotic terminator vector (very similar to pSB1AK8)
creator: iGEM Team Freiburg 2008
created: 2008-10-25T11:00:00Z
modified: 2015-05-08T01:10:55Z
discontinued: false
dominant: false
experience: experience/None
group u list: _232_
m user id: 0
owner id: 1673
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Transfection vector with CMV Promotor
mutable notes: none
mutable provenance: BBa_J52017 and CMV Promotor
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: terminator
sequence annotation: kanamycin resistance marker
sequence annotation: beta-lactamase (ampicillin resistance marker)
sequence annotation: rep pMB(1)
sequence annotation: BioBrick prefix partial (missing G at 3' end)
sequence annotation: BioBrick suffix
sequence: https://synbiohub.org/public/igem/BBa_K157040_sequence/1

terminator
type: SequenceAnnotation
persistent identity: annotation1999641
display id: annotation1999641
version: 1
top level: Eukaryotic
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K157040/annotation1999641/range1999641/1

https://synbiohub.org/public/igem/BBa_K157040/annotation1999641/range1999641/1
type: Range
persistent identity: range1999641
display id: range1999641
version: 1
direction: direction/forward
top level: Eukaryotic
start: 22
end: 259
orientation: inline

kanamycin resistance marker
type: SequenceAnnotation
persistent identity: annotation1999643
display id: annotation1999643
version: 1
top level: Eukaryotic
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K157040/annotation1999643/range1999643/1

https://synbiohub.org/public/igem/BBa_K157040/annotation1999643/range1999643/1
type: Range
persistent identity: range1999643
display id: range1999643
version: 1
direction: direction/reverse
top level: Eukaryotic
start: 1334
end: 2149
orientation: inline

beta-lactamase (ampicillin resistance marker)
type: SequenceAnnotation
persistent identity: annotation1999644
display id: annotation1999644
version: 1
top level: Eukaryotic
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K157040/annotation1999644/range1999644/1

https://synbiohub.org/public/igem/BBa_K157040/annotation1999644/range1999644/1
type: Range
persistent identity: range1999644
display id: range1999644
version: 1
direction: direction/reverse
top level: Eukaryotic
start: 2304
end: 3164
orientation: inline

rep pMB(1)
type: SequenceAnnotation
persistent identity: annotation1999642
display id: annotation1999642
version: 1
top level: Eukaryotic
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K157040/annotation1999642/range1999642/1

https://synbiohub.org/public/igem/BBa_K157040/annotation1999642/range1999642/1
type: Range
persistent identity: range1999642
display id: range1999642
version: 1
direction: direction/reverse
top level: Eukaryotic
start: 498
end: 1112
orientation: inline

BioBrick prefix partial (missing G at 3' end)
type: SequenceAnnotation
persistent identity: annotation1999652
display id: annotation1999652
version: 1
top level: Eukaryotic
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K157040/annotation1999652/range1999652/1

https://synbiohub.org/public/igem/BBa_K157040/annotation1999652/range1999652/1
type: Range
persistent identity: range1999652
display id: range1999652
version: 1
direction: direction/forward
top level: Eukaryotic
start: 3405
end: 3425
orientation: inline

BioBrick suffix
type: SequenceAnnotation
persistent identity: annotation1992676
display id: annotation1992676
version: 1
top level: Eukaryotic
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K157040/annotation1992676/range1992676/1

https://synbiohub.org/public/igem/BBa_K157040/annotation1992676/range1992676/1
type: Range
persistent identity: range1992676
display id: range1992676
version: 1
direction: direction/forward
top level: Eukaryotic
start: 1
end: 21
orientation: inline

BBa_J179000
type: ComponentDefinition
persistent identity: BBa J179000
display id: BBa_J179000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pBS1C: Bacillus subtilis vector, amyE integration, CAM-resistance
creator: Jara Radeck
created: 2013-12-03T12:00:00Z
modified: 2015-08-31T04:08:35Z
discontinued: false
dominant: false
experience: experience/None
group u list: _865_
m user id: 0
owner id: 6378
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This vector integrates into the amyE-site of Bacillus subtilis via double recombination. The two recombination sites flank a standard BioBrick multiple cloning site (with RFP-cassette), as well as a chloramphenicol resistance cassette (5 ??g/ml). The backbone does not contain the restriction sites EcoRI, XbaI, SpeI, PstI, NgoMIV nor AgeI. The vector is replicative in Escherichia coli and provides resistance to ampicillin (100 ??g/ml). Before transformation into B. subtilis, this vector should be linearized with ScaI.
mutable notes: This is one of three empty vectors with compatible integration loci and resistance cassettes to allow combination of up to three constructs of choice in one single strain.
mutable provenance: pDG1731
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: ori
sequence annotation: amyE-front
sequence annotation: cat
sequence annotation: bla
sequence annotation: amyE-back
sequence: https://synbiohub.org/public/igem/BBa_J179000_sequence/1

ori
type: SequenceAnnotation
persistent identity: annotation2371577
display id: annotation2371577
version: 1
top level: BBa_J179000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J179000/annotation2371577/range2371577/1

https://synbiohub.org/public/igem/BBa_J179000/annotation2371577/range2371577/1
type: Range
persistent identity: range2371577
display id: range2371577
version: 1
direction: direction/forward
top level: BBa_J179000
start: 3587
end: 4201
orientation: inline

amyE-front
type: SequenceAnnotation
persistent identity: annotation2371576
display id: annotation2371576
version: 1
top level: BBa_J179000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J179000/annotation2371576/range2371576/1

https://synbiohub.org/public/igem/BBa_J179000/annotation2371576/range2371576/1
type: Range
persistent identity: range2371576
display id: range2371576
version: 1
direction: direction/forward
top level: BBa_J179000
start: 5546
end: 6066
orientation: inline

cat
type: SequenceAnnotation
persistent identity: annotation2371573
display id: annotation2371573
version: 1
top level: BBa_J179000
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J179000/annotation2371573/range2371573/1

https://synbiohub.org/public/igem/BBa_J179000/annotation2371573/range2371573/1
type: Range
persistent identity: range2371573
display id: range2371573
version: 1
direction: direction/forward
top level: BBa_J179000
start: 358
end: 1008
orientation: inline

bla
type: SequenceAnnotation
persistent identity: annotation2371575
display id: annotation2371575
version: 1
top level: BBa_J179000
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J179000/annotation2371575/range2371575/1

https://synbiohub.org/public/igem/BBa_J179000/annotation2371575/range2371575/1
type: Range
persistent identity: range2371575
display id: range2371575
version: 1
direction: direction/reverse
top level: BBa_J179000
start: 4361
end: 5221
orientation: inline

amyE-back
type: SequenceAnnotation
persistent identity: annotation2371574
display id: annotation2371574
version: 1
top level: BBa_J179000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J179000/annotation2371574/range2371574/1

https://synbiohub.org/public/igem/BBa_J179000/annotation2371574/range2371574/1
type: Range
persistent identity: range2371574
display id: range2371574
version: 1
direction: direction/forward
top level: BBa_J179000
start: 1379
end: 2407
orientation: inline

pSB4C5
type: ComponentDefinition
persistent identity: pSB4C5
display id: pSB4C5
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Low copy BioBrick standard vector
creator: Reshma Shetty
created: 2007-02-26T12:00:00Z
modified: 2015-05-08T01:14:55Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _41_
m user id: 0
owner id: 126
owning group id: 70
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: pSB4C3 is a low copy plasmid based with chloramphenicol resistance. It has a repA pSC101-derived replication origin. (Copy number ~10-12, Lutz and Bujard, 1997.) pSB4C5 is derived from the BioBricks vector scaffold BBa_I51001. However, the direction of the resistance marker and replication origin is not optimal.
mutable notes: None.
mutable provenance: BBa_I51001 BioBricks vector scaffold, pSC101 based origin and a version of the chloramphenicol rsistance cassette that has been cleaned of restriction sites.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
component: BBa_B0053
component: BBa_P1004
component: BBa_B0062
component: BBa_B0042
component: BBa_B0055
component: BBa_B0054
component: BBa_G0004
component: BBa_G00100
component: BBa_G0003
component: BBa_G0002
component: BBa_G00001
component: BBa_B0043
component: BBa_G00000
component: BBa_B0044
component: BBa_I50042
component: BBa_G00102
sequence annotation: BBa_G00100
sequence annotation: BBa_G00001
sequence annotation: BBa_G00102
sequence annotation: BBa_P1004
sequence annotation: BBa_G0003
sequence annotation: BBa_B0053
sequence annotation: BBa_G0002
sequence annotation: BBa_B0042
sequence annotation: BBa_G00000
sequence annotation: BBa_G0004
sequence annotation: BBa_B0054
sequence annotation: BBa_B0055
sequence annotation: BBa_B0062
sequence annotation: BBa_B0044
sequence annotation: BBa_I50042
sequence annotation: BBa_B0043
sequence: https://synbiohub.org/public/igem/pSB4C5_sequence/1

BBa_B0053
type: Component
persistent identity: component2236290
display id: component2236290
version: 1
top level: pSB4C5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0053/1

BBa_P1004
type: Component
persistent identity: component2236287
display id: component2236287
version: 1
top level: pSB4C5
access: public
definition: https://synbiohub.org/public/igem/BBa_P1004/1

BBa_B0062
type: Component
persistent identity: component2236255
display id: component2236255
version: 1
top level: pSB4C5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0062/1

BBa_B0042
type: Component
persistent identity: component2236248
display id: component2236248
version: 1
top level: pSB4C5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0042/1

BBa_B0055
type: Component
persistent identity: component2236295
display id: component2236295
version: 1
top level: pSB4C5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0055/1

BBa_B0054
type: Component
persistent identity: component2236251
display id: component2236251
version: 1
top level: pSB4C5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0054/1

BBa_G0004
type: Component
persistent identity: component2236288
display id: component2236288
version: 1
top level: pSB4C5
access: public
definition: https://synbiohub.org/public/igem/BBa_G0004/1

BBa_G00100
type: Component
persistent identity: component2236292
display id: component2236292
version: 1
top level: pSB4C5
access: public
definition: https://synbiohub.org/public/igem/BBa_G00100/1

BBa_B0042
type: Component
persistent identity: component2236302
display id: component2236302
version: 1
top level: pSB4C5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0042/1

BBa_G0003
type: Component
persistent identity: component2236256
display id: component2236256
version: 1
top level: pSB4C5
access: public
definition: https://synbiohub.org/public/igem/BBa_G0003/1

BBa_G0002
type: Component
persistent identity: component2236285
display id: component2236285
version: 1
top level: pSB4C5
access: public
definition: https://synbiohub.org/public/igem/BBa_G0002/1

BBa_G00001
type: Component
persistent identity: component2236236
display id: component2236236
version: 1
top level: pSB4C5
access: public
definition: https://synbiohub.org/public/igem/BBa_G00001/1

BBa_B0043
type: Component
persistent identity: component2236307
display id: component2236307
version: 1
top level: pSB4C5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0043/1

BBa_G00000
type: Component
persistent identity: component2236312
display id: component2236312
version: 1
top level: pSB4C5
access: public
definition: https://synbiohub.org/public/igem/BBa_G00000/1

BBa_B0044
type: Component
persistent identity: component2236241
display id: component2236241
version: 1
top level: pSB4C5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0044/1

BBa_I50042
type: Component
persistent identity: component2236284
display id: component2236284
version: 1
top level: pSB4C5
access: public
definition: https://synbiohub.org/public/igem/BBa_I50042/1

BBa_G00102
type: Component
persistent identity: component2236253
display id: component2236253
version: 1
top level: pSB4C5
access: public
definition: https://synbiohub.org/public/igem/BBa_G00102/1

BBa_G00100
type: SequenceAnnotation
persistent identity: annotation2236292
display id: annotation2236292
version: 1
top level: pSB4C5
location: https://synbiohub.org/public/igem/pSB4C5/annotation2236292/range2236292/1
component: BBa_G00100

https://synbiohub.org/public/igem/pSB4C5/annotation2236292/range2236292/1
type: Range
persistent identity: range2236292
display id: range2236292
version: 1
direction: direction/reverse
top level: pSB4C5
start: 3077
end: 3096
orientation: inline

BBa_G00001
type: SequenceAnnotation
persistent identity: annotation2236236
display id: annotation2236236
version: 1
top level: pSB4C5
location: https://synbiohub.org/public/igem/pSB4C5/annotation2236236/range2236236/1
component: BBa_G00001

https://synbiohub.org/public/igem/pSB4C5/annotation2236236/range2236236/1
type: Range
persistent identity: range2236236
display id: range2236236
version: 1
direction: direction/reverse
top level: pSB4C5
start: 1
end: 21
orientation: inline

BBa_G00102
type: SequenceAnnotation
persistent identity: annotation2236253
display id: annotation2236253
version: 1
top level: pSB4C5
location: https://synbiohub.org/public/igem/pSB4C5/annotation2236253/range2236253/1
component: BBa_G00102

https://synbiohub.org/public/igem/pSB4C5/annotation2236253/range2236253/1
type: Range
persistent identity: range2236253
display id: range2236253
version: 1
direction: direction/reverse
top level: pSB4C5
start: 116
end: 135
orientation: inline

BBa_P1004
type: SequenceAnnotation
persistent identity: annotation2236287
display id: annotation2236287
version: 1
top level: pSB4C5
location: https://synbiohub.org/public/igem/pSB4C5/annotation2236287/range2236287/1
component: BBa_P1004

https://synbiohub.org/public/igem/pSB4C5/annotation2236287/range2236287/1
type: Range
persistent identity: range2236287
display id: range2236287
version: 1
direction: direction/reverse
top level: pSB4C5
start: 2229
end: 2997
orientation: inline

BBa_G0003
type: SequenceAnnotation
persistent identity: annotation2236256
display id: annotation2236256
version: 1
top level: pSB4C5
location: https://synbiohub.org/public/igem/pSB4C5/annotation2236256/range2236256/1
component: BBa_G0003

https://synbiohub.org/public/igem/pSB4C5/annotation2236256/range2236256/1
type: Range
persistent identity: range2236256
display id: range2236256
version: 1
direction: direction/reverse
top level: pSB4C5
start: 177
end: 183
orientation: inline

BBa_B0053
type: SequenceAnnotation
persistent identity: annotation2236290
display id: annotation2236290
version: 1
top level: pSB4C5
location: https://synbiohub.org/public/igem/pSB4C5/annotation2236290/range2236290/1
component: BBa_B0053

https://synbiohub.org/public/igem/pSB4C5/annotation2236290/range2236290/1
type: Range
persistent identity: range2236290
display id: range2236290
version: 1
direction: direction/reverse
top level: pSB4C5
start: 3005
end: 3076
orientation: inline

BBa_G0002
type: SequenceAnnotation
persistent identity: annotation2236285
display id: annotation2236285
version: 1
top level: pSB4C5
location: https://synbiohub.org/public/igem/pSB4C5/annotation2236285/range2236285/1
component: BBa_G0002

https://synbiohub.org/public/igem/pSB4C5/annotation2236285/range2236285/1
type: Range
persistent identity: range2236285
display id: range2236285
version: 1
direction: direction/reverse
top level: pSB4C5
start: 2221
end: 2228
orientation: inline

BBa_B0042
type: SequenceAnnotation
persistent identity: annotation2236302
display id: annotation2236302
version: 1
top level: pSB4C5
location: https://synbiohub.org/public/igem/pSB4C5/annotation2236302/range2236302/1
component: BBa_B0042

https://synbiohub.org/public/igem/pSB4C5/annotation2236302/range2236302/1
type: Range
persistent identity: range2236302
display id: range2236302
version: 1
direction: direction/reverse
top level: pSB4C5
start: 3175
end: 3186
orientation: inline

BBa_G00000
type: SequenceAnnotation
persistent identity: annotation2236312
display id: annotation2236312
version: 1
top level: pSB4C5
location: https://synbiohub.org/public/igem/pSB4C5/annotation2236312/range2236312/1
component: BBa_G00000

https://synbiohub.org/public/igem/pSB4C5/annotation2236312/range2236312/1
type: Range
persistent identity: range2236312
display id: range2236312
version: 1
direction: direction/reverse
top level: pSB4C5
start: 3200
end: 3221
orientation: inline

BBa_G0004
type: SequenceAnnotation
persistent identity: annotation2236288
display id: annotation2236288
version: 1
top level: pSB4C5
location: https://synbiohub.org/public/igem/pSB4C5/annotation2236288/range2236288/1
component: BBa_G0004

https://synbiohub.org/public/igem/pSB4C5/annotation2236288/range2236288/1
type: Range
persistent identity: range2236288
display id: range2236288
version: 1
direction: direction/reverse
top level: pSB4C5
start: 2998
end: 3004
orientation: inline

BBa_B0042
type: SequenceAnnotation
persistent identity: annotation2236248
display id: annotation2236248
version: 1
top level: pSB4C5
location: https://synbiohub.org/public/igem/pSB4C5/annotation2236248/range2236248/1
component: BBa_B0042

https://synbiohub.org/public/igem/pSB4C5/annotation2236248/range2236248/1
type: Range
persistent identity: range2236248
display id: range2236248
version: 1
direction: direction/reverse
top level: pSB4C5
start: 35
end: 46
orientation: inline

BBa_B0054
type: SequenceAnnotation
persistent identity: annotation2236251
display id: annotation2236251
version: 1
top level: pSB4C5
location: https://synbiohub.org/public/igem/pSB4C5/annotation2236251/range2236251/1
component: BBa_B0054

https://synbiohub.org/public/igem/pSB4C5/annotation2236251/range2236251/1
type: Range
persistent identity: range2236251
display id: range2236251
version: 1
direction: direction/reverse
top level: pSB4C5
start: 47
end: 115
orientation: inline

BBa_B0055
type: SequenceAnnotation
persistent identity: annotation2236295
display id: annotation2236295
version: 1
top level: pSB4C5
location: https://synbiohub.org/public/igem/pSB4C5/annotation2236295/range2236295/1
component: BBa_B0055

https://synbiohub.org/public/igem/pSB4C5/annotation2236295/range2236295/1
type: Range
persistent identity: range2236295
display id: range2236295
version: 1
direction: direction/reverse
top level: pSB4C5
start: 3097
end: 3174
orientation: inline

BBa_B0062
type: SequenceAnnotation
persistent identity: annotation2236255
display id: annotation2236255
version: 1
top level: pSB4C5
location: https://synbiohub.org/public/igem/pSB4C5/annotation2236255/range2236255/1
component: BBa_B0062

https://synbiohub.org/public/igem/pSB4C5/annotation2236255/range2236255/1
type: Range
persistent identity: range2236255
display id: range2236255
version: 1
direction: direction/reverse
top level: pSB4C5
start: 136
end: 176
orientation: inline

BBa_B0044
type: SequenceAnnotation
persistent identity: annotation2236241
display id: annotation2236241
version: 1
top level: pSB4C5
location: https://synbiohub.org/public/igem/pSB4C5/annotation2236241/range2236241/1
component: BBa_B0044

https://synbiohub.org/public/igem/pSB4C5/annotation2236241/range2236241/1
type: Range
persistent identity: range2236241
display id: range2236241
version: 1
direction: direction/reverse
top level: pSB4C5
start: 22
end: 34
orientation: inline

BBa_I50042
type: SequenceAnnotation
persistent identity: annotation2236284
display id: annotation2236284
version: 1
top level: pSB4C5
location: https://synbiohub.org/public/igem/pSB4C5/annotation2236284/range2236284/1
component: BBa_I50042

https://synbiohub.org/public/igem/pSB4C5/annotation2236284/range2236284/1
type: Range
persistent identity: range2236284
display id: range2236284
version: 1
direction: direction/reverse
top level: pSB4C5
start: 184
end: 2220
orientation: inline

BBa_B0043
type: SequenceAnnotation
persistent identity: annotation2236307
display id: annotation2236307
version: 1
top level: pSB4C5
location: https://synbiohub.org/public/igem/pSB4C5/annotation2236307/range2236307/1
component: BBa_B0043

https://synbiohub.org/public/igem/pSB4C5/annotation2236307/range2236307/1
type: Range
persistent identity: range2236307
display id: range2236307
version: 1
direction: direction/reverse
top level: pSB4C5
start: 3187
end: 3199
orientation: inline

pSB1AK8
type: ComponentDefinition
persistent identity: pSB1AK8
display id: pSB1AK8
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Eukaryotic terminator vector (formerly BBa_J52017)
creator: Monika Ciglic
created: 2006-10-13T11:00:00Z
modified: 2015-05-08T01:14:55Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _80_
m user id: 0
owner id: 800
owning group id: 80
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This vector is made of BioBrick vector pSB1AK3, which has eucaryotic terminator added.
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: rep (pMB1)
sequence annotation: BioBrick suffix
sequence annotation: kanR
sequence annotation: terminator
sequence annotation: BioBrick prefix
sequence annotation: ampR
sequence: https://synbiohub.org/public/igem/pSB1AK8_sequence/1

rep (pMB1)
type: SequenceAnnotation
persistent identity: annotation1999648
display id: annotation1999648
version: 1
top level: pSB1AK8
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/pSB1AK8/annotation1999648/range1999648/1

https://synbiohub.org/public/igem/pSB1AK8/annotation1999648/range1999648/1
type: Range
persistent identity: range1999648
display id: range1999648
version: 1
direction: direction/reverse
top level: pSB1AK8
start: 498
end: 1112
orientation: inline

BioBrick suffix
type: SequenceAnnotation
persistent identity: annotation1999646
display id: annotation1999646
version: 1
top level: pSB1AK8
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/pSB1AK8/annotation1999646/range1999646/1

https://synbiohub.org/public/igem/pSB1AK8/annotation1999646/range1999646/1
type: Range
persistent identity: range1999646
display id: range1999646
version: 1
direction: direction/forward
top level: pSB1AK8
start: 1
end: 21
orientation: inline

kanR
type: SequenceAnnotation
persistent identity: annotation1999649
display id: annotation1999649
version: 1
top level: pSB1AK8
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/pSB1AK8/annotation1999649/range1999649/1

https://synbiohub.org/public/igem/pSB1AK8/annotation1999649/range1999649/1
type: Range
persistent identity: range1999649
display id: range1999649
version: 1
direction: direction/reverse
top level: pSB1AK8
start: 1334
end: 2149
orientation: inline

terminator
type: SequenceAnnotation
persistent identity: annotation1999647
display id: annotation1999647
version: 1
top level: pSB1AK8
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/pSB1AK8/annotation1999647/range1999647/1

https://synbiohub.org/public/igem/pSB1AK8/annotation1999647/range1999647/1
type: Range
persistent identity: range1999647
display id: range1999647
version: 1
direction: direction/forward
top level: pSB1AK8
start: 22
end: 259
orientation: inline

BioBrick prefix
type: SequenceAnnotation
persistent identity: annotation1999651
display id: annotation1999651
version: 1
top level: pSB1AK8
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/pSB1AK8/annotation1999651/range1999651/1

https://synbiohub.org/public/igem/pSB1AK8/annotation1999651/range1999651/1
type: Range
persistent identity: range1999651
display id: range1999651
version: 1
direction: direction/forward
top level: pSB1AK8
start: 3405
end: 3426
orientation: inline

ampR
type: SequenceAnnotation
persistent identity: annotation1999650
display id: annotation1999650
version: 1
top level: pSB1AK8
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/pSB1AK8/annotation1999650/range1999650/1

https://synbiohub.org/public/igem/pSB1AK8/annotation1999650/range1999650/1
type: Range
persistent identity: range1999650
display id: range1999650
version: 1
direction: direction/reverse
top level: pSB1AK8
start: 2304
end: 3164
orientation: inline

BBa_K864014
type: ComponentDefinition
persistent identity: BBa K864014
display id: BBa_K864014
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Low copy BioBrick standard vector with LacI
creator: Erik Lundin
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:37Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1124_
m user id: 0
owner id: 9827
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: pSB4A15Iq(FRT) is a BioBrick standard vector with low copy pSC101 replication origin (~5 copies per cell) and ampicillin antibiotic resistance marker, especially usable for Lambda Red recombineering in E coli. The backbone sequence is based on pSB3T5, but the E coli His operon terminator BBa_B0053 has been replaced with the late terminator of the Salmonella phage P22. This vector carries a LacIq cassette on the backbone for tight repression of any lac promoter cloned into the cloning site.
mutable notes: The resistance cassette is terminated by a Lux bidirectional terminator. Adjacent to this terminator there is a designed primer binding site to where the forward primer for lambda red can be designed to bind to no matter what the insert in the cloning site is or what the resistance is. The resistance cassette is flanked by SalI and SacI restriction sites for easy switching of resistance in the backbone.
mutable provenance: Based on BBa_K864006
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence: https://synbiohub.org/public/igem/BBa_K864014_sequence/1

pSB8A15
type: ComponentDefinition
persistent identity: BBa K864016
display id: BBa_K864016
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Low copy BioBrick temperature sensitive standard vector
creator: Erik Lundin
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:37Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1124_
m user id: 0
owner id: 9827
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: pSB8A15 is a BioBrick standard vector with low copy pSC101ts replication origin (~5 copies per cell) and ampicillin antibiotic resistance marker, especially usable for Lambda Red recombineering in E coli. The pSC101ts is a temperature sensitive pSC101 repelication origin and propagates at 30???C but stop replicating at 42???C. The backbone sequence is based on pSB3T5, but the E coli His operon terminator BBa_B0053 has been replaced with the late terminator of the Salmonella phage P22.
mutable notes: The resistance cassette is terminated by a Lux bidirectional terminator. Adjacent to this terminator there is a designed primer binding site to where the forward primer for lambda red can be designed to bind to no matter what the insert in the cloning site is or what the resistance is. The resistance cassette is flanked by SalI and SacI restriction sites for easy switching of resistance in the backbone. The pSC101ts origin of replication is flaked by NheI and MluI restriction sites for easy switching of origin of replication.
mutable provenance: Based on BBa_K864000
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence: https://synbiohub.org/public/igem/BBa_K864016_sequence/1

BBa_J64100
type: ComponentDefinition
persistent identity: BBa J64100
display id: BBa_J64100
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Medium copy ColE1 BioBrick vector
creator: Jeffrey J Tabor
created: 2007-04-02T11:00:00Z
modified: 2015-06-15T12:42:46Z
discontinued: true
dominant: false
experience: experience/Works
group u list: _98_
m user id: 4206
owner id: 88
owning group id: 98
sample status: Not in stock
status: status/Deleted
bookmark: false
mutable description: This plasmid is based on pProTetE333 (clontech). A SpeI site was removed via an A-T mutation at position 1166 on the original plasmid, resulting in pProTetE333b. The G00100-G00101 fragment of pSB4A3-1 which includes P1010 between a biobricks multiple cloning site which itself is flanked by + strand transcriptional terminators was then cloned between the XhoI and AvrII sites of pProTetE333b. The resulting plasmid carries a ColE1 origin of replication, a Chloramphenicol resistance marker, the biobricks MCS and the ccdAB death cassette.
mutable notes: None
mutable provenance: pProTetE333 (clontech) and biobricks parts
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: prefix
sequence annotation: ColE1 origin
sequence annotation: G00101
sequence annotation: G00100
sequence annotation: chloramphenicol resistance marker
sequence annotation: suffix
sequence annotation: P1010
sequence: https://synbiohub.org/public/igem/BBa_J64100_sequence/1

prefix
type: SequenceAnnotation
persistent identity: annotation1923841
display id: annotation1923841
version: 1
top level: BBa_J64100
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_J64100/annotation1923841/range1923841/1

https://synbiohub.org/public/igem/BBa_J64100/annotation1923841/range1923841/1
type: Range
persistent identity: range1923841
display id: range1923841
version: 1
direction: direction/forward
top level: BBa_J64100
start: 124
end: 145
orientation: inline

ColE1 origin
type: SequenceAnnotation
persistent identity: annotation1923845
display id: annotation1923845
version: 1
top level: BBa_J64100
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J64100/annotation1923845/range1923845/1

https://synbiohub.org/public/igem/BBa_J64100/annotation1923845/range1923845/1
type: Range
persistent identity: range1923845
display id: range1923845
version: 1
direction: direction/reverse
top level: BBa_J64100
start: 1130
end: 1812
orientation: inline

G00101
type: SequenceAnnotation
persistent identity: annotation1923844
display id: annotation1923844
version: 1
top level: BBa_J64100
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_J64100/annotation1923844/range1923844/1

https://synbiohub.org/public/igem/BBa_J64100/annotation1923844/range1923844/1
type: Range
persistent identity: range1923844
display id: range1923844
version: 1
direction: direction/reverse
top level: BBa_J64100
start: 977
end: 996
orientation: inline

G00100
type: SequenceAnnotation
persistent identity: annotation1923840
display id: annotation1923840
version: 1
top level: BBa_J64100
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_J64100/annotation1923840/range1923840/1

https://synbiohub.org/public/igem/BBa_J64100/annotation1923840/range1923840/1
type: Range
persistent identity: range1923840
display id: range1923840
version: 1
direction: direction/forward
top level: BBa_J64100
start: 6
end: 25
orientation: inline

G00101
type: SequenceAnnotation
persistent identity: annotation1931328
display id: annotation1931328
version: 1
top level: BBa_J64100
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_J64100/annotation1931328/range1931328/1

https://synbiohub.org/public/igem/BBa_J64100/annotation1931328/range1931328/1
type: Range
persistent identity: range1931328
display id: range1931328
version: 1
direction: direction/reverse
top level: BBa_J64100
start: 1029
end: 1048
orientation: inline

chloramphenicol resistance marker
type: SequenceAnnotation
persistent identity: annotation1923846
display id: annotation1923846
version: 1
top level: BBa_J64100
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J64100/annotation1923846/range1923846/1

https://synbiohub.org/public/igem/BBa_J64100/annotation1923846/range1923846/1
type: Range
persistent identity: range1923846
display id: range1923846
version: 1
direction: direction/reverse
top level: BBa_J64100
start: 1939
end: 2598
orientation: inline

suffix
type: SequenceAnnotation
persistent identity: annotation1923843
display id: annotation1923843
version: 1
top level: BBa_J64100
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_J64100/annotation1923843/range1923843/1

https://synbiohub.org/public/igem/BBa_J64100/annotation1923843/range1923843/1
type: Range
persistent identity: range1923843
display id: range1923843
version: 1
direction: direction/forward
top level: BBa_J64100
start: 821
end: 841
orientation: inline

P1010
type: SequenceAnnotation
persistent identity: annotation1923842
display id: annotation1923842
version: 1
top level: BBa_J64100
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_J64100/annotation1923842/range1923842/1

https://synbiohub.org/public/igem/BBa_J64100/annotation1923842/range1923842/1
type: Range
persistent identity: range1923842
display id: range1923842
version: 1
direction: direction/forward
top level: BBa_J64100
start: 146
end: 820
orientation: inline

BBa_K864015
type: ComponentDefinition
persistent identity: BBa K864015
display id: BBa_K864015
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Low copy BioBrick standard vector with LacI
creator: Erik Lundin
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:37Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1124_
m user id: 0
owner id: 9827
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: pSB4S15Iq(FRT) is a BioBrick standard vector with low copy pSC101 replication origin (~5 copies per cell) and spectinomycin antibiotic resistance marker, especially usable for Lambda Red recombineering in E coli. The backbone sequence is based on pSB3T5, but the E coli His operon terminator BBa_B0053 has been replaced with the late terminator of the Salmonella phage P22. This vector carries a LacIq cassette on the backbone for tight repression of any lac promoter cloned into the cloning site.
mutable notes: The resistance cassette is terminated by a Lux bidirectional terminator. Adjacent to this terminator there is a designed primer binding site to where the forward primer for lambda red can be designed to bind to no matter what the insert in the cloning site is or what the resistance is. The resistance cassette is flanked by SalI and SacI restriction sites for easy switching of resistance in the backbone.
mutable provenance: Based on BBa_K864007
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence: https://synbiohub.org/public/igem/BBa_K864015_sequence/1

BBa_J179002
type: ComponentDefinition
persistent identity: BBa J179002
display id: BBa_J179002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pBs4S: Bacillus subtilis vector, thrC integration, spec resistance
creator: Jara Radeck
created: 2013-12-20T12:00:00Z
modified: 2015-08-31T04:08:35Z
discontinued: false
dominant: false
group u list: _865_
m user id: 0
owner id: 6378
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This part is an empty backbone vector for the usage in Bacillus subtilis. It integrates in the thrC locus and can be selected with Spectinomycin cassette. It has a ampicillin resistance for cloning in E.coli. This backbone is a BioBricked version of the B. subtilis vector pDG1731.
mutable notes: This vector is compatible with pBS1C and pBS2E in one strain.
mutable provenance: This BioBrick is part of the LMU 2012 igem project BacillusBioBrickBox For handling of B. subtilis vectors, please see here. The transformation into B. subtilis is explained here.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: hom, trC front
sequence annotation: spc
sequence annotation: bla
sequence annotation: thrC,thrB back
sequence annotation: ori
sequence: https://synbiohub.org/public/igem/BBa_J179002_sequence/1

hom, trC front
type: SequenceAnnotation
persistent identity: annotation2371770
display id: annotation2371770
version: 1
top level: BBa_J179002
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J179002/annotation2371770/range2371770/1

https://synbiohub.org/public/igem/BBa_J179002/annotation2371770/range2371770/1
type: Range
persistent identity: range2371770
display id: range2371770
version: 1
direction: direction/forward
top level: BBa_J179002
start: 3731
end: 4542
orientation: inline

spc
type: SequenceAnnotation
persistent identity: annotation2371766
display id: annotation2371766
version: 1
top level: BBa_J179002
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J179002/annotation2371766/range2371766/1

https://synbiohub.org/public/igem/BBa_J179002/annotation2371766/range2371766/1
type: Range
persistent identity: range2371766
display id: range2371766
version: 1
direction: direction/forward
top level: BBa_J179002
start: 211
end: 885
orientation: inline

bla
type: SequenceAnnotation
persistent identity: annotation2371769
display id: annotation2371769
version: 1
top level: BBa_J179002
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J179002/annotation2371769/range2371769/1

https://synbiohub.org/public/igem/BBa_J179002/annotation2371769/range2371769/1
type: Range
persistent identity: range2371769
display id: range2371769
version: 1
direction: direction/reverse
top level: BBa_J179002
start: 2716
end: 3576
orientation: inline

thrC,thrB back
type: SequenceAnnotation
persistent identity: annotation2371767
display id: annotation2371767
version: 1
top level: BBa_J179002
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J179002/annotation2371767/range2371767/1

https://synbiohub.org/public/igem/BBa_J179002/annotation2371767/range2371767/1
type: Range
persistent identity: range2371767
display id: range2371767
version: 1
direction: direction/forward
top level: BBa_J179002
start: 1028
end: 1863
orientation: inline

ori
type: SequenceAnnotation
persistent identity: annotation2371768
display id: annotation2371768
version: 1
top level: BBa_J179002
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J179002/annotation2371768/range2371768/1

https://synbiohub.org/public/igem/BBa_J179002/annotation2371768/range2371768/1
type: Range
persistent identity: range2371768
display id: range2371768
version: 1
direction: direction/forward
top level: BBa_J179002
start: 1942
end: 2556
orientation: inline

BBa_K864003
type: ComponentDefinition
persistent identity: BBa K864003
display id: BBa_K864003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Low copy BioBrick standard vector
creator: Erik Lundin
created: 2012-09-22T11:00:00Z
modified: 2015-05-08T01:13:37Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1124_
m user id: 0
owner id: 9827
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: pSB4A15 is a BioBrick standard vector with low copy pSC101 replication origin (~5 copies per cell) and ampicillin antibiotic resistance marker, especially usable for Lambda Red recombineering in E coli. The backbone sequence is based on pSB3T5, but the E coli His operon terminator BBa_B0053 has been replaced with the late terminator of the Salmonella phage P22.
mutable notes: The resistance cassette is terminated by a Lux bidirectional terminator. Adjacent to this terminator there is a designed primer binding site to where the forward primer for lambda red can be designed to bind to no matter what the insert in the cloning site is or what the resistance is. The resistance cassette is flanked by SalI and SacI restriction sites for easy switching of resistance in the backbone. The pSC101 origin of replication is flaked by NheI and MluI restriction sites for easy switching of origin of replication.
mutable provenance: Based on pSB3T5
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: VF2
sequence annotation: Lambda Red forward primer
sequence annotation: T3 terminator
sequence annotation: P22 late terminator
sequence annotation: Promoter
sequence annotation: Lux terminator
sequence annotation: pSC101 ori BBa_K864050
sequence annotation: Lambda Red reverse primer
sequence annotation: TonB terminator
sequence annotation: spcR
sequence annotation: VR
sequence annotation: rnC terminator
sequence: https://synbiohub.org/public/igem/BBa_K864003_sequence/1

VF2
type: SequenceAnnotation
persistent identity: annotation2201244
display id: annotation2201244
version: 1
top level: BBa_K864003
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K864003/annotation2201244/range2201244/1

https://synbiohub.org/public/igem/BBa_K864003/annotation2201244/range2201244/1
type: Range
persistent identity: range2201244
display id: range2201244
version: 1
direction: direction/forward
top level: BBa_K864003
start: 3649
end: 3668
orientation: inline

Lambda Red forward primer
type: SequenceAnnotation
persistent identity: annotation2201239
display id: annotation2201239
version: 1
top level: BBa_K864003
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K864003/annotation2201239/range2201239/1

https://synbiohub.org/public/igem/BBa_K864003/annotation2201239/range2201239/1
type: Range
persistent identity: range2201239
display id: range2201239
version: 1
direction: direction/forward
top level: BBa_K864003
start: 2433
end: 2450
orientation: inline

T3 terminator
type: SequenceAnnotation
persistent identity: annotation2201245
display id: annotation2201245
version: 1
top level: BBa_K864003
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K864003/annotation2201245/range2201245/1

https://synbiohub.org/public/igem/BBa_K864003/annotation2201245/range2201245/1
type: Range
persistent identity: range2201245
display id: range2201245
version: 1
direction: direction/forward
top level: BBa_K864003
start: 3669
end: 3746
orientation: inline

P22 late terminator
type: SequenceAnnotation
persistent identity: annotation2201243
display id: annotation2201243
version: 1
top level: BBa_K864003
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K864003/annotation2201243/range2201243/1

https://synbiohub.org/public/igem/BBa_K864003/annotation2201243/range2201243/1
type: Range
persistent identity: range2201243
display id: range2201243
version: 1
direction: direction/forward
top level: BBa_K864003
start: 3608
end: 3648
orientation: inline

Promoter
type: SequenceAnnotation
persistent identity: annotation2201242
display id: annotation2201242
version: 1
top level: BBa_K864003
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K864003/annotation2201242/range2201242/1

https://synbiohub.org/public/igem/BBa_K864003/annotation2201242/range2201242/1
type: Range
persistent identity: range2201242
display id: range2201242
version: 1
direction: direction/reverse
top level: BBa_K864003
start: 3575
end: 3601
orientation: inline

Lux terminator
type: SequenceAnnotation
persistent identity: annotation2201240
display id: annotation2201240
version: 1
top level: BBa_K864003
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K864003/annotation2201240/range2201240/1

https://synbiohub.org/public/igem/BBa_K864003/annotation2201240/range2201240/1
type: Range
persistent identity: range2201240
display id: range2201240
version: 1
direction: direction/reverse
top level: BBa_K864003
start: 2454
end: 2499
orientation: inline

pSC101 ori BBa_K864050
type: SequenceAnnotation
persistent identity: annotation2201238
display id: annotation2201238
version: 1
top level: BBa_K864003
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864003/annotation2201238/range2201238/1

https://synbiohub.org/public/igem/BBa_K864003/annotation2201238/range2201238/1
type: Range
persistent identity: range2201238
display id: range2201238
version: 1
direction: direction/forward
top level: BBa_K864003
start: 190
end: 2405
orientation: inline

Lambda Red reverse primer
type: SequenceAnnotation
persistent identity: annotation2201234
display id: annotation2201234
version: 1
top level: BBa_K864003
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K864003/annotation2201234/range2201234/1

https://synbiohub.org/public/igem/BBa_K864003/annotation2201234/range2201234/1
type: Range
persistent identity: range2201234
display id: range2201234
version: 1
direction: direction/reverse
top level: BBa_K864003
start: 18
end: 38
orientation: inline

TonB terminator
type: SequenceAnnotation
persistent identity: annotation2201235
display id: annotation2201235
version: 1
top level: BBa_K864003
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K864003/annotation2201235/range2201235/1

https://synbiohub.org/public/igem/BBa_K864003/annotation2201235/range2201235/1
type: Range
persistent identity: range2201235
display id: range2201235
version: 1
direction: direction/forward
top level: BBa_K864003
start: 47
end: 115
orientation: inline

spcR
type: SequenceAnnotation
persistent identity: annotation2201241
display id: annotation2201241
version: 1
top level: BBa_K864003
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K864003/annotation2201241/range2201241/1

https://synbiohub.org/public/igem/BBa_K864003/annotation2201241/range2201241/1
type: Range
persistent identity: range2201241
display id: range2201241
version: 1
direction: direction/reverse
top level: BBa_K864003
start: 2510
end: 3508
orientation: inline

VR
type: SequenceAnnotation
persistent identity: annotation2201236
display id: annotation2201236
version: 1
top level: BBa_K864003
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K864003/annotation2201236/range2201236/1

https://synbiohub.org/public/igem/BBa_K864003/annotation2201236/range2201236/1
type: Range
persistent identity: range2201236
display id: range2201236
version: 1
direction: direction/forward
top level: BBa_K864003
start: 116
end: 135
orientation: inline

rnC terminator
type: SequenceAnnotation
persistent identity: annotation2201237
display id: annotation2201237
version: 1
top level: BBa_K864003
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K864003/annotation2201237/range2201237/1

https://synbiohub.org/public/igem/BBa_K864003/annotation2201237/range2201237/1
type: Range
persistent identity: range2201237
display id: range2201237
version: 1
direction: direction/forward
top level: BBa_K864003
start: 136
end: 175
orientation: inline

pSB8S15FRT
type: ComponentDefinition
persistent identity: BBa K864023
display id: BBa_K864023
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Low copy BioBrick temperature sensitive standard vector
creator: Erik Lundin
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:37Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1124_
m user id: 0
owner id: 9827
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: pSB8S15(FRT) is a BioBrick standard vector with low copy pSC101ts replication origin (~5 copies per cell) and spectinomycin antibiotic resistance marker, especially usable for Lambda Red recombineering in E coli. The pSC101ts is a temperature sensitive pSC101 repelication origin and propagates at 30???C but stop replicating at 42???C. The backbone sequence is based on pSB3T5, but the E coli His operon terminator BBa_B0053 has been replaced with the late terminator of the Salmonella phage P22.
mutable notes: The resistance cassette is terminated by a Lux bidirectional terminator. Adjacent to this terminator there is a designed primer binding site to where the forward primer for lambda red can be designed to bind to no matter what the insert in the cloning site is or what the resistance is. The resistance cassette is flanked by SalI and SacI restriction sites for easy switching of resistance in the backbone. The pSC101ts origin of replication is flaked by NheI and MluI restriction sites for easy switching of origin of replication.
mutable provenance: Based on BBa_K864017
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence: https://synbiohub.org/public/igem/BBa_K864023_sequence/1

BBa_K864001
type: ComponentDefinition
persistent identity: BBa K864001
display id: BBa_K864001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Low copy BioBrick standard vector
creator: Erik Lundin
created: 2012-09-22T11:00:00Z
modified: 2015-05-08T01:13:37Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1124_
m user id: 0
owner id: 9827
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: pSB4C15 is a BioBrick standard vector with low copy pSC101 replication origin (~5 copies per cell) and chloramphenicol antibiotic resistance marker, especially usable for Lambda Red recombineering in E coli. The backbone sequence is based on pSB3T5, but the E coli His operon terminator BBa_B0053 has been replaced with the late terminator of the Salmonella phage P22.
mutable notes: The resistance cassette is terminated by a Lux bidirectional terminator. Adjacent to this terminator there is a designed primer binding site to where the forward primer for lambda red can be designed to bind to no matter what the insert in the cloning site is or what the resistance is. The resistance cassette is flanked by SalI and SacI restriction sites for easy switching of resistance in the backbone. The pSC101 origin of replication is flaked by NheI and MluI restriction sites for easy switching of origin of replication.
mutable provenance: Based on pSB3T5
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: Lambda Red forward primer
sequence annotation: P22 late terminator
sequence annotation: VR
sequence annotation: Tandem repeat
sequence annotation: Lambda Red reverse primer
sequence annotation: Lux terminator
sequence annotation: CmR BBa_P1004
sequence annotation: tonB terminator
sequence annotation: repA
sequence annotation: Repeat "4"
sequence annotation: Repeat "5"
sequence annotation: T3 terminator
sequence annotation: pSC101 ori
sequence annotation: -35
sequence annotation: rrnC terminator
sequence annotation: -10
sequence annotation: Mutated A>T to remove SpeI
sequence annotation: VF2
sequence annotation: Promoter
sequence: https://synbiohub.org/public/igem/BBa_K864001_sequence/1

Lambda Red forward primer
type: SequenceAnnotation
persistent identity: annotation2198606
display id: annotation2198606
version: 1
top level: BBa_K864001
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K864001/annotation2198606/range2198606/1

https://synbiohub.org/public/igem/BBa_K864001/annotation2198606/range2198606/1
type: Range
persistent identity: range2198606
display id: range2198606
version: 1
direction: direction/forward
top level: BBa_K864001
start: 2433
end: 2450
orientation: inline

P22 late terminator
type: SequenceAnnotation
persistent identity: annotation2198628
display id: annotation2198628
version: 1
top level: BBa_K864001
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K864001/annotation2198628/range2198628/1

https://synbiohub.org/public/igem/BBa_K864001/annotation2198628/range2198628/1
type: Range
persistent identity: range2198628
display id: range2198628
version: 1
direction: direction/forward
top level: BBa_K864001
start: 3251
end: 3321
orientation: inline

VR
type: SequenceAnnotation
persistent identity: annotation2198565
display id: annotation2198565
version: 1
top level: BBa_K864001
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K864001/annotation2198565/range2198565/1

https://synbiohub.org/public/igem/BBa_K864001/annotation2198565/range2198565/1
type: Range
persistent identity: range2198565
display id: range2198565
version: 1
direction: direction/reverse
top level: BBa_K864001
start: 116
end: 135
orientation: inline

Tandem repeat
type: SequenceAnnotation
persistent identity: annotation2198576
display id: annotation2198576
version: 1
top level: BBa_K864001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864001/annotation2198576/range2198576/1

https://synbiohub.org/public/igem/BBa_K864001/annotation2198576/range2198576/1
type: Range
persistent identity: range2198576
display id: range2198576
version: 1
direction: direction/forward
top level: BBa_K864001
start: 790
end: 809
orientation: inline

Lambda Red reverse primer
type: SequenceAnnotation
persistent identity: annotation2198340
display id: annotation2198340
version: 1
top level: BBa_K864001
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K864001/annotation2198340/range2198340/1

https://synbiohub.org/public/igem/BBa_K864001/annotation2198340/range2198340/1
type: Range
persistent identity: range2198340
display id: range2198340
version: 1
direction: direction/reverse
top level: BBa_K864001
start: 18
end: 38
orientation: inline

Lux terminator
type: SequenceAnnotation
persistent identity: annotation2198616
display id: annotation2198616
version: 1
top level: BBa_K864001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864001/annotation2198616/range2198616/1

https://synbiohub.org/public/igem/BBa_K864001/annotation2198616/range2198616/1
type: Range
persistent identity: range2198616
display id: range2198616
version: 1
direction: direction/reverse
top level: BBa_K864001
start: 2454
end: 2499
orientation: inline

CmR BBa_P1004
type: SequenceAnnotation
persistent identity: annotation2198620
display id: annotation2198620
version: 1
top level: BBa_K864001
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K864001/annotation2198620/range2198620/1

https://synbiohub.org/public/igem/BBa_K864001/annotation2198620/range2198620/1
type: Range
persistent identity: range2198620
display id: range2198620
version: 1
direction: direction/reverse
top level: BBa_K864001
start: 2506
end: 3168
orientation: inline

tonB terminator
type: SequenceAnnotation
persistent identity: annotation2198561
display id: annotation2198561
version: 1
top level: BBa_K864001
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K864001/annotation2198561/range2198561/1

https://synbiohub.org/public/igem/BBa_K864001/annotation2198561/range2198561/1
type: Range
persistent identity: range2198561
display id: range2198561
version: 1
direction: direction/forward
top level: BBa_K864001
start: 47
end: 115
orientation: inline

repA
type: SequenceAnnotation
persistent identity: annotation2198605
display id: annotation2198605
version: 1
top level: BBa_K864001
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K864001/annotation2198605/range2198605/1

https://synbiohub.org/public/igem/BBa_K864001/annotation2198605/range2198605/1
type: Range
persistent identity: range2198605
display id: range2198605
version: 1
direction: direction/forward
top level: BBa_K864001
start: 939
end: 1889
orientation: inline

Repeat "4"
type: SequenceAnnotation
persistent identity: annotation2198580
display id: annotation2198580
version: 1
top level: BBa_K864001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864001/annotation2198580/range2198580/1

https://synbiohub.org/public/igem/BBa_K864001/annotation2198580/range2198580/1
type: Range
persistent identity: range2198580
display id: range2198580
version: 1
direction: direction/forward
top level: BBa_K864001
start: 862
end: 880
orientation: inline

Repeat "5"
type: SequenceAnnotation
persistent identity: annotation2198585
display id: annotation2198585
version: 1
top level: BBa_K864001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864001/annotation2198585/range2198585/1

https://synbiohub.org/public/igem/BBa_K864001/annotation2198585/range2198585/1
type: Range
persistent identity: range2198585
display id: range2198585
version: 1
direction: direction/forward
top level: BBa_K864001
start: 891
end: 912
orientation: inline

T3 terminator
type: SequenceAnnotation
persistent identity: annotation2198614
display id: annotation2198614
version: 1
top level: BBa_K864001
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K864001/annotation2198614/range2198614/1

https://synbiohub.org/public/igem/BBa_K864001/annotation2198614/range2198614/1
type: Range
persistent identity: range2198614
display id: range2198614
version: 1
direction: direction/forward
top level: BBa_K864001
start: 3342
end: 3419
orientation: inline

pSC101 ori
type: SequenceAnnotation
persistent identity: annotation2198568
display id: annotation2198568
version: 1
top level: BBa_K864001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864001/annotation2198568/range2198568/1

https://synbiohub.org/public/igem/BBa_K864001/annotation2198568/range2198568/1
type: Range
persistent identity: range2198568
display id: range2198568
version: 1
direction: direction/forward
top level: BBa_K864001
start: 190
end: 2405
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2198584
display id: annotation2198584
version: 1
top level: BBa_K864001
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K864001/annotation2198584/range2198584/1

https://synbiohub.org/public/igem/BBa_K864001/annotation2198584/range2198584/1
type: Range
persistent identity: range2198584
display id: range2198584
version: 1
direction: direction/forward
top level: BBa_K864001
start: 881
end: 886
orientation: inline

rrnC terminator
type: SequenceAnnotation
persistent identity: annotation2198566
display id: annotation2198566
version: 1
top level: BBa_K864001
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K864001/annotation2198566/range2198566/1

https://synbiohub.org/public/igem/BBa_K864001/annotation2198566/range2198566/1
type: Range
persistent identity: range2198566
display id: range2198566
version: 1
direction: direction/forward
top level: BBa_K864001
start: 136
end: 175
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2198603
display id: annotation2198603
version: 1
top level: BBa_K864001
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K864001/annotation2198603/range2198603/1

https://synbiohub.org/public/igem/BBa_K864001/annotation2198603/range2198603/1
type: Range
persistent identity: range2198603
display id: range2198603
version: 1
direction: direction/forward
top level: BBa_K864001
start: 903
end: 908
orientation: inline

Mutated A>T to remove SpeI
type: SequenceAnnotation
persistent identity: annotation2198582
display id: annotation2198582
version: 1
top level: BBa_K864001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864001/annotation2198582/range2198582/1

https://synbiohub.org/public/igem/BBa_K864001/annotation2198582/range2198582/1
type: Range
persistent identity: range2198582
display id: range2198582
version: 1
direction: direction/forward
top level: BBa_K864001
start: 867
end: 867
orientation: inline

VF2
type: SequenceAnnotation
persistent identity: annotation2198629
display id: annotation2198629
version: 1
top level: BBa_K864001
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K864001/annotation2198629/range2198629/1

https://synbiohub.org/public/igem/BBa_K864001/annotation2198629/range2198629/1
type: Range
persistent identity: range2198629
display id: range2198629
version: 1
direction: direction/forward
top level: BBa_K864001
start: 3322
end: 3341
orientation: inline

Tandem repeat
type: SequenceAnnotation
persistent identity: annotation2198574
display id: annotation2198574
version: 1
top level: BBa_K864001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864001/annotation2198574/range2198574/1

https://synbiohub.org/public/igem/BBa_K864001/annotation2198574/range2198574/1
type: Range
persistent identity: range2198574
display id: range2198574
version: 1
direction: direction/forward
top level: BBa_K864001
start: 767
end: 788
orientation: inline

Promoter
type: SequenceAnnotation
persistent identity: annotation2198622
display id: annotation2198622
version: 1
top level: BBa_K864001
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K864001/annotation2198622/range2198622/1

https://synbiohub.org/public/igem/BBa_K864001/annotation2198622/range2198622/1
type: Range
persistent identity: range2198622
display id: range2198622
version: 1
direction: direction/reverse
top level: BBa_K864001
start: 3235
end: 3261
orientation: inline

Tandem repeat
type: SequenceAnnotation
persistent identity: annotation2198578
display id: annotation2198578
version: 1
top level: BBa_K864001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864001/annotation2198578/range2198578/1

https://synbiohub.org/public/igem/BBa_K864001/annotation2198578/range2198578/1
type: Range
persistent identity: range2198578
display id: range2198578
version: 1
direction: direction/forward
top level: BBa_K864001
start: 820
end: 841
orientation: inline

pSac-Cm_BB
type: ComponentDefinition
persistent identity: BBa K818000
display id: BBa_K818000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Integration vector for Bacillus subtilis derived from pSac-Cm
creator: Emeraldo Jo
created: 2012-08-21T11:00:00Z
modified: 2015-05-08T01:13:29Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1076_
m user id: 0
owner id: 11906
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: The plasmid is designed to integrate a cloned insert into the B. subtilis 168 chromosome at the sacA locus. The user inserts the fragment inside the MCS whi
mutable notes: Biobrick ready
mutable provenance: MolGen Group (University of Groningen)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: BioBrick prefix
sequence annotation: sacA'
sequence annotation: BioBrick suffix
sequence annotation: bla
sequence annotation: 'sacA
sequence annotation: BBa_B0015
sequence annotation: cat
sequence: https://synbiohub.org/public/igem/BBa_K818000_sequence/1

BioBrick prefix
type: SequenceAnnotation
persistent identity: annotation2203458
display id: annotation2203458
version: 1
top level: pSac-Cm_BB
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K818000/annotation2203458/range2203458/1

https://synbiohub.org/public/igem/BBa_K818000/annotation2203458/range2203458/1
type: Range
persistent identity: range2203458
display id: range2203458
version: 1
direction: direction/forward
top level: pSac-Cm_BB
start: 5207
end: 5223
orientation: inline

sacA'
type: SequenceAnnotation
persistent identity: annotation2203456
display id: annotation2203456
version: 1
top level: pSac-Cm_BB
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K818000/annotation2203456/range2203456/1

https://synbiohub.org/public/igem/BBa_K818000/annotation2203456/range2203456/1
type: Range
persistent identity: range2203456
display id: range2203456
version: 1
direction: direction/forward
top level: pSac-Cm_BB
start: 3187
end: 3930
orientation: inline

BioBrick suffix
type: SequenceAnnotation
persistent identity: annotation2203452
display id: annotation2203452
version: 1
top level: pSac-Cm_BB
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K818000/annotation2203452/range2203452/1

https://synbiohub.org/public/igem/BBa_K818000/annotation2203452/range2203452/1
type: Range
persistent identity: range2203452
display id: range2203452
version: 1
direction: direction/forward
top level: pSac-Cm_BB
start: 1
end: 17
orientation: inline

bla
type: SequenceAnnotation
persistent identity: annotation2203455
display id: annotation2203455
version: 1
top level: pSac-Cm_BB
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K818000/annotation2203455/range2203455/1

https://synbiohub.org/public/igem/BBa_K818000/annotation2203455/range2203455/1
type: Range
persistent identity: range2203455
display id: range2203455
version: 1
direction: direction/forward
top level: pSac-Cm_BB
start: 1324
end: 2196
orientation: inline

'sacA
type: SequenceAnnotation
persistent identity: annotation2203454
display id: annotation2203454
version: 1
top level: pSac-Cm_BB
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K818000/annotation2203454/range2203454/1

https://synbiohub.org/public/igem/BBa_K818000/annotation2203454/range2203454/1
type: Range
persistent identity: range2203454
display id: range2203454
version: 1
direction: direction/forward
top level: pSac-Cm_BB
start: 181
end: 990
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2203453
display id: annotation2203453
version: 1
top level: pSac-Cm_BB
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K818000/annotation2203453/range2203453/1

https://synbiohub.org/public/igem/BBa_K818000/annotation2203453/range2203453/1
type: Range
persistent identity: range2203453
display id: range2203453
version: 1
direction: direction/forward
top level: pSac-Cm_BB
start: 18
end: 170
orientation: inline

cat
type: SequenceAnnotation
persistent identity: annotation2203457
display id: annotation2203457
version: 1
top level: pSac-Cm_BB
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K818000/annotation2203457/range2203457/1

https://synbiohub.org/public/igem/BBa_K818000/annotation2203457/range2203457/1
type: Range
persistent identity: range2203457
display id: range2203457
version: 1
direction: direction/reverse
top level: pSac-Cm_BB
start: 4249
end: 4911
orientation: inline

pSB4C1
type: ComponentDefinition
persistent identity: pSB4C1
display id: pSB4C1
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pCK01 converted into BioBrick vector (formerly BBa_I739202)
creator: Stefan Luzi
created: 2007-10-23T11:00:00Z
modified: 2015-05-08T01:14:55Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _124_
m user id: 0
owner id: 2183
owning group id: 62
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: asdf
mutable notes: asdf
mutable provenance: asdf
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: BioBrick prefix
sequence annotation: C->T mutation to eliminate SpeI site
sequence annotation: pSC101 replication initiator protein
sequence annotation: chloramphenicol acetyltransferase
sequence annotation: BioBrick suffix
sequence annotation: Mutagenesis primer
sequence: https://synbiohub.org/public/igem/pSB4C1_sequence/1

BioBrick prefix
type: SequenceAnnotation
persistent identity: annotation1969636
display id: annotation1969636
version: 1
top level: pSB4C1
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/pSB4C1/annotation1969636/range1969636/1

https://synbiohub.org/public/igem/pSB4C1/annotation1969636/range1969636/1
type: Range
persistent identity: range1969636
display id: range1969636
version: 1
direction: direction/forward
top level: pSB4C1
start: 3134
end: 3155
orientation: inline

C->T mutation to eliminate SpeI site
type: SequenceAnnotation
persistent identity: annotation1969634
display id: annotation1969634
version: 1
top level: pSB4C1
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/pSB4C1/annotation1969634/range1969634/1

https://synbiohub.org/public/igem/pSB4C1/annotation1969634/range1969634/1
type: Range
persistent identity: range1969634
display id: range1969634
version: 1
direction: direction/forward
top level: pSB4C1
start: 1508
end: 1508
orientation: inline

pSC101 replication initiator protein
type: SequenceAnnotation
persistent identity: annotation1969632
display id: annotation1969632
version: 1
top level: pSB4C1
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/pSB4C1/annotation1969632/range1969632/1

https://synbiohub.org/public/igem/pSB4C1/annotation1969632/range1969632/1
type: Range
persistent identity: range1969632
display id: range1969632
version: 1
direction: direction/reverse
top level: pSB4C1
start: 490
end: 1440
orientation: inline

chloramphenicol acetyltransferase
type: SequenceAnnotation
persistent identity: annotation1969633
display id: annotation1969633
version: 1
top level: pSB4C1
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/pSB4C1/annotation1969633/range1969633/1

https://synbiohub.org/public/igem/pSB4C1/annotation1969633/range1969633/1
type: Range
persistent identity: range1969633
display id: range1969633
version: 1
direction: direction/forward
top level: pSB4C1
start: 2311
end: 2970
orientation: inline

BioBrick suffix
type: SequenceAnnotation
persistent identity: annotation1969631
display id: annotation1969631
version: 1
top level: pSB4C1
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/pSB4C1/annotation1969631/range1969631/1

https://synbiohub.org/public/igem/pSB4C1/annotation1969631/range1969631/1
type: Range
persistent identity: range1969631
display id: range1969631
version: 1
direction: direction/forward
top level: pSB4C1
start: 1
end: 21
orientation: inline

Mutagenesis primer
type: SequenceAnnotation
persistent identity: annotation1969635
display id: annotation1969635
version: 1
top level: pSB4C1
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/pSB4C1/annotation1969635/range1969635/1

https://synbiohub.org/public/igem/pSB4C1/annotation1969635/range1969635/1
type: Range
persistent identity: range1969635
display id: range1969635
version: 1
direction: direction/reverse
top level: pSB4C1
start: 1491
end: 1529
orientation: inline

pSB3K3
type: ComponentDefinition
persistent identity: pSB3K3
display id: pSB3K3
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Low to medium copy BioBrick standard vector
creator: Reshma Shetty
created: 2004-06-28T11:00:00Z
modified: 2015-05-08T01:14:55Z
discontinued: false
dominant: false
experience: experience/Works
m user id: 0
owner id: 60
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: pSB3K3-1 is a medium copy plasmid with kanamycin resistance. It has a p15A pMR101-derived replication origin, copy number 20-30. (Lutz and Bujard, 1997) pSB3K3-1 has a terminator upstream of its MCS, which is oriented to prevent transcription from *inside* the MCS from reading out into the vectorl. A second terminator (E.coli His operon-derived) is downstream of the MCS, again insulating the vector from transcription reading out of the MCS. Ideally, future versions of standard biobrick vectors would have terminators bracketing the MCS that were 100% efficient in terminating transcription both into and out of the MCS region.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: kanR (rev)
sequence annotation: E. coli his operon terminator
sequence annotation: Prefix
sequence annotation: VR
sequence annotation: deletion of two bases
sequence annotation: terminator stem loop
sequence annotation: VF2
sequence annotation: p15A ORI (rev)
sequence annotation: end of terminator
sequence annotation: Suffix
sequence: https://synbiohub.org/public/igem/pSB3K3_sequence/1

kanR (rev)
type: SequenceAnnotation
persistent identity: annotation1023045
display id: annotation1023045
version: 1
top level: pSB3K3
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/pSB3K3/annotation1023045/range1023045/1

https://synbiohub.org/public/igem/pSB3K3/annotation1023045/range1023045/1
type: Range
persistent identity: range1023045
display id: range1023045
version: 1
direction: direction/reverse
top level: pSB3K3
start: 239
end: 1054
orientation: inline

E. coli his operon terminator
type: SequenceAnnotation
persistent identity: annotation1824021
display id: annotation1824021
version: 1
top level: pSB3K3
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB3K3/annotation1824021/range1824021/1

https://synbiohub.org/public/igem/pSB3K3/annotation1824021/range1824021/1
type: Range
persistent identity: range1824021
display id: range1824021
version: 1
direction: direction/forward
top level: pSB3K3
start: 22
end: 93
orientation: inline

Prefix
type: SequenceAnnotation
persistent identity: annotation1023043
display id: annotation1023043
version: 1
top level: pSB3K3
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/pSB3K3/annotation1023043/range1023043/1

https://synbiohub.org/public/igem/pSB3K3/annotation1023043/range1023043/1
type: Range
persistent identity: range1023043
display id: range1023043
version: 1
direction: direction/forward
top level: pSB3K3
start: 2729
end: 2750
orientation: inline

VR
type: SequenceAnnotation
persistent identity: annotation1782476
display id: annotation1782476
version: 1
top level: pSB3K3
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/pSB3K3/annotation1782476/range1782476/1

https://synbiohub.org/public/igem/pSB3K3/annotation1782476/range1782476/1
type: Range
persistent identity: range1782476
display id: range1782476
version: 1
direction: direction/reverse
top level: pSB3K3
start: 157
end: 176
orientation: inline

deletion of two bases
type: SequenceAnnotation
persistent identity: annotation1823788
display id: annotation1823788
version: 1
top level: pSB3K3
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB3K3/annotation1823788/range1823788/1

https://synbiohub.org/public/igem/pSB3K3/annotation1823788/range1823788/1
type: Range
persistent identity: range1823788
display id: range1823788
version: 1
direction: direction/forward
top level: pSB3K3
start: 34
end: 35
orientation: inline

terminator stem loop
type: SequenceAnnotation
persistent identity: annotation1824022
display id: annotation1824022
version: 1
top level: pSB3K3
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/pSB3K3/annotation1824022/range1824022/1

https://synbiohub.org/public/igem/pSB3K3/annotation1824022/range1824022/1
type: Range
persistent identity: range1824022
display id: range1824022
version: 1
direction: direction/forward
top level: pSB3K3
start: 30
end: 64
orientation: inline

VF2
type: SequenceAnnotation
persistent identity: annotation1782459
display id: annotation1782459
version: 1
top level: pSB3K3
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/pSB3K3/annotation1782459/range1782459/1

https://synbiohub.org/public/igem/pSB3K3/annotation1782459/range1782459/1
type: Range
persistent identity: range1782459
display id: range1782459
version: 1
direction: direction/forward
top level: pSB3K3
start: 2611
end: 2630
orientation: inline

p15A ORI (rev)
type: SequenceAnnotation
persistent identity: annotation1023046
display id: annotation1023046
version: 1
top level: pSB3K3
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/pSB3K3/annotation1023046/range1023046/1

https://synbiohub.org/public/igem/pSB3K3/annotation1023046/range1023046/1
type: Range
persistent identity: range1023046
display id: range1023046
version: 1
direction: direction/reverse
top level: pSB3K3
start: 1386
end: 2210
orientation: inline

end of terminator
type: SequenceAnnotation
persistent identity: annotation1824024
display id: annotation1824024
version: 1
top level: pSB3K3
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB3K3/annotation1824024/range1824024/1

https://synbiohub.org/public/igem/pSB3K3/annotation1824024/range1824024/1
type: Range
persistent identity: range1824024
display id: range1824024
version: 1
direction: direction/reverse
top level: pSB3K3
start: 2687
end: 2690
orientation: inline

Suffix
type: SequenceAnnotation
persistent identity: annotation1023044
display id: annotation1023044
version: 1
top level: pSB3K3
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/pSB3K3/annotation1023044/range1023044/1

https://synbiohub.org/public/igem/pSB3K3/annotation1023044/range1023044/1
type: Range
persistent identity: range1023044
display id: range1023044
version: 1
direction: direction/forward
top level: pSB3K3
start: 1
end: 21
orientation: inline

terminator stem loop
type: SequenceAnnotation
persistent identity: annotation1824023
display id: annotation1824023
version: 1
top level: pSB3K3
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/pSB3K3/annotation1824023/range1824023/1

https://synbiohub.org/public/igem/pSB3K3/annotation1824023/range1824023/1
type: Range
persistent identity: range1824023
display id: range1824023
version: 1
direction: direction/reverse
top level: pSB3K3
start: 2700
end: 2719
orientation: inline

K592200
type: ComponentDefinition
persistent identity: BBa K592200
display id: BBa_K592200
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Low to medium copy BioBrick standard vector
creator: Erik Gullberg, Antonio Ascue Avalos, Erik Lundin
created: 2011-09-20T11:00:00Z
modified: 2015-05-08T01:12:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _763_
m user id: 0
owner id: 10133
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: BBa_K592200 is a BioBrick standard vector with low to medium copy p15A replication origin (BBa_I50032) and spectinomycin antibiotic resistance marker, usable for Lambda Red recombineering in E coli. The E coli His operon terminator BBa_B0053 has been replaced with the late terminator of the Salmonella phage P22.
mutable notes: To make the plasmid usable for Lambda Red recombineering, all sequences with homologies to the E coli chromosome had to be removed. To accomplish this, the E coli His operon terminator BBa_B0053 has been replaced with the late terminator of the Salmonella phage P22.
mutable provenance: Salmonella phage P22 late terminator.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: Spectinomycin resistance marker
sequence annotation: Terminator BBa_B0054
sequence annotation: Translational stop BBa_B0042
sequence annotation: Lambda red F primer
sequence annotation: VR site BBa_G00102
sequence annotation: T3 terminator BBa_B0055
sequence annotation: p15A low to medium copy replication origin BBa_I50032
sequence annotation: P22 late terminator
sequence annotation: VF2 site BBa_G00100
sequence annotation: Terminator BBa_B0062
sequence annotation: Lambda Red R primer
sequence annotation: SpcR promoter
sequence: https://synbiohub.org/public/igem/BBa_K592200_sequence/1

Spectinomycin resistance marker
type: SequenceAnnotation
persistent identity: annotation2136375
display id: annotation2136375
version: 1
top level: K592200
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K592200/annotation2136375/range2136375/1

https://synbiohub.org/public/igem/BBa_K592200/annotation2136375/range2136375/1
type: Range
persistent identity: range2136375
display id: range2136375
version: 1
direction: direction/reverse
top level: K592200
start: 1841
end: 2840
orientation: inline

Terminator BBa_B0054
type: SequenceAnnotation
persistent identity: annotation2136370
display id: annotation2136370
version: 1
top level: K592200
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K592200/annotation2136370/range2136370/1

https://synbiohub.org/public/igem/BBa_K592200/annotation2136370/range2136370/1
type: Range
persistent identity: range2136370
display id: range2136370
version: 1
direction: direction/forward
top level: K592200
start: 47
end: 115
orientation: inline

Translational stop BBa_B0042
type: SequenceAnnotation
persistent identity: annotation2136380
display id: annotation2136380
version: 1
top level: K592200
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K592200/annotation2136380/range2136380/1

https://synbiohub.org/public/igem/BBa_K592200/annotation2136380/range2136380/1
type: Range
persistent identity: range2136380
display id: range2136380
version: 1
direction: direction/forward
top level: K592200
start: 3091
end: 3102
orientation: inline

Lambda red F primer
type: SequenceAnnotation
persistent identity: annotation2136374
display id: annotation2136374
version: 1
top level: K592200
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K592200/annotation2136374/range2136374/1

https://synbiohub.org/public/igem/BBa_K592200/annotation2136374/range2136374/1
type: Range
persistent identity: range2136374
display id: range2136374
version: 1
direction: direction/forward
top level: K592200
start: 1726
end: 1745
orientation: inline

VR site BBa_G00102
type: SequenceAnnotation
persistent identity: annotation2136371
display id: annotation2136371
version: 1
top level: K592200
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K592200/annotation2136371/range2136371/1

https://synbiohub.org/public/igem/BBa_K592200/annotation2136371/range2136371/1
type: Range
persistent identity: range2136371
display id: range2136371
version: 1
direction: direction/reverse
top level: K592200
start: 116
end: 135
orientation: inline

T3 terminator BBa_B0055
type: SequenceAnnotation
persistent identity: annotation2136379
display id: annotation2136379
version: 1
top level: K592200
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K592200/annotation2136379/range2136379/1

https://synbiohub.org/public/igem/BBa_K592200/annotation2136379/range2136379/1
type: Range
persistent identity: range2136379
display id: range2136379
version: 1
direction: direction/forward
top level: K592200
start: 3013
end: 3090
orientation: inline

p15A low to medium copy replication origin BBa_I50032
type: SequenceAnnotation
persistent identity: annotation2136373
display id: annotation2136373
version: 1
top level: K592200
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K592200/annotation2136373/range2136373/1

https://synbiohub.org/public/igem/BBa_K592200/annotation2136373/range2136373/1
type: Range
persistent identity: range2136373
display id: range2136373
version: 1
direction: direction/forward
top level: K592200
start: 184
end: 1748
orientation: inline

P22 late terminator
type: SequenceAnnotation
persistent identity: annotation2136377
display id: annotation2136377
version: 1
top level: K592200
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K592200/annotation2136377/range2136377/1

https://synbiohub.org/public/igem/BBa_K592200/annotation2136377/range2136377/1
type: Range
persistent identity: range2136377
display id: range2136377
version: 1
direction: direction/forward
top level: K592200
start: 2952
end: 2992
orientation: inline

VF2 site BBa_G00100
type: SequenceAnnotation
persistent identity: annotation2136378
display id: annotation2136378
version: 1
top level: K592200
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K592200/annotation2136378/range2136378/1

https://synbiohub.org/public/igem/BBa_K592200/annotation2136378/range2136378/1
type: Range
persistent identity: range2136378
display id: range2136378
version: 1
direction: direction/forward
top level: K592200
start: 2993
end: 3012
orientation: inline

Terminator BBa_B0062
type: SequenceAnnotation
persistent identity: annotation2136372
display id: annotation2136372
version: 1
top level: K592200
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K592200/annotation2136372/range2136372/1

https://synbiohub.org/public/igem/BBa_K592200/annotation2136372/range2136372/1
type: Range
persistent identity: range2136372
display id: range2136372
version: 1
direction: direction/forward
top level: K592200
start: 136
end: 176
orientation: inline

Lambda Red R primer
type: SequenceAnnotation
persistent identity: annotation2136398
display id: annotation2136398
version: 1
top level: K592200
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K592200/annotation2136398/range2136398/1

https://synbiohub.org/public/igem/BBa_K592200/annotation2136398/range2136398/1
type: Range
persistent identity: range2136398
display id: range2136398
version: 1
direction: direction/reverse
top level: K592200
start: 20
end: 41
orientation: inline

Translational stop BBa_B0042
type: SequenceAnnotation
persistent identity: annotation2136369
display id: annotation2136369
version: 1
top level: K592200
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K592200/annotation2136369/range2136369/1

https://synbiohub.org/public/igem/BBa_K592200/annotation2136369/range2136369/1
type: Range
persistent identity: range2136369
display id: range2136369
version: 1
direction: direction/forward
top level: K592200
start: 35
end: 46
orientation: inline

SpcR promoter
type: SequenceAnnotation
persistent identity: annotation2136376
display id: annotation2136376
version: 1
top level: K592200
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K592200/annotation2136376/range2136376/1

https://synbiohub.org/public/igem/BBa_K592200/annotation2136376/range2136376/1
type: Range
persistent identity: range2136376
display id: range2136376
version: 1
direction: direction/reverse
top level: K592200
start: 2907
end: 2933
orientation: inline

pSB4A50
type: ComponentDefinition
persistent identity: BBa K1362095
display id: BBa_K1362095
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pSB4A50: Low copy BioBrick cloning/expression backbone carrying Amp resistance
creator: Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena B??scher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Sch??fer, Carolin Schmelas, Silvan Schmitz, Max Waldha
created: 2014-10-07T11:00:00Z
modified: 2015-05-08T01:10:04Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1738_
m user id: 0
owner id: 22830
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
component: BBa_G00100
component: BBa_G00001
component: BBa_G0004
component: BBa_B0054
component: BBa_G00000
component: BBa_I50042
component: BBa_K1362463
component: BBa_B0055
component: BBa_B0043
component: BBa_B0042
component: BBa_B0044
component: BBa_B0062
component: BBa_G00102
component: BBa_G0002
component: BBa_G0003
component: BBa_P1002
component: BBa_B0053
sequence annotation: BBa_B0053
sequence annotation: BBa_K1362463
sequence annotation: BBa_B0055
sequence annotation: BBa_B0042
sequence annotation: BBa_G0003
sequence annotation: BBa_B0043
sequence annotation: BBa_G00100
sequence annotation: BBa_G0002
sequence annotation: BBa_G00102
sequence annotation: BBa_B0044
sequence annotation: BBa_G00001
sequence annotation: BBa_P1002
sequence annotation: BBa_I50042
sequence annotation: BBa_G0004
sequence annotation: BBa_B0054
sequence annotation: BBa_B0062
sequence annotation: BBa_G00000
sequence: https://synbiohub.org/public/igem/BBa_K1362095_sequence/1

BBa_G00100
type: Component
persistent identity: component2409823
display id: component2409823
version: 1
top level: pSB4A50
access: public
definition: https://synbiohub.org/public/igem/BBa_G00100/1

BBa_G00001
type: Component
persistent identity: component2409764
display id: component2409764
version: 1
top level: pSB4A50
access: public
definition: https://synbiohub.org/public/igem/BBa_G00001/1

BBa_G0004
type: Component
persistent identity: component2409819
display id: component2409819
version: 1
top level: pSB4A50
access: public
definition: https://synbiohub.org/public/igem/BBa_G0004/1

BBa_B0054
type: Component
persistent identity: component2409779
display id: component2409779
version: 1
top level: pSB4A50
access: public
definition: https://synbiohub.org/public/igem/BBa_B0054/1

BBa_G00000
type: Component
persistent identity: component2409847
display id: component2409847
version: 1
top level: pSB4A50
access: public
definition: https://synbiohub.org/public/igem/BBa_G00000/1

BBa_I50042
type: Component
persistent identity: component2409812
display id: component2409812
version: 1
top level: pSB4A50
access: public
definition: https://synbiohub.org/public/igem/BBa_I50042/1

BBa_K1362463
type: Component
persistent identity: component2409842
display id: component2409842
version: 1
top level: pSB4A50
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362463/1

BBa_B0055
type: Component
persistent identity: component2409826
display id: component2409826
version: 1
top level: pSB4A50
access: public
definition: https://synbiohub.org/public/igem/BBa_B0055/1

BBa_B0043
type: Component
persistent identity: component2409838
display id: component2409838
version: 1
top level: pSB4A50
access: public
definition: https://synbiohub.org/public/igem/BBa_B0043/1

BBa_B0042
type: Component
persistent identity: component2409776
display id: component2409776
version: 1
top level: pSB4A50
access: public
definition: https://synbiohub.org/public/igem/BBa_B0042/1

BBa_B0044
type: Component
persistent identity: component2409769
display id: component2409769
version: 1
top level: pSB4A50
access: public
definition: https://synbiohub.org/public/igem/BBa_B0044/1

BBa_B0062
type: Component
persistent identity: component2409783
display id: component2409783
version: 1
top level: pSB4A50
access: public
definition: https://synbiohub.org/public/igem/BBa_B0062/1

BBa_G00102
type: Component
persistent identity: component2409781
display id: component2409781
version: 1
top level: pSB4A50
access: public
definition: https://synbiohub.org/public/igem/BBa_G00102/1

BBa_G0002
type: Component
persistent identity: component2409813
display id: component2409813
version: 1
top level: pSB4A50
access: public
definition: https://synbiohub.org/public/igem/BBa_G0002/1

BBa_G0003
type: Component
persistent identity: component2409784
display id: component2409784
version: 1
top level: pSB4A50
access: public
definition: https://synbiohub.org/public/igem/BBa_G0003/1

BBa_B0042
type: Component
persistent identity: component2409833
display id: component2409833
version: 1
top level: pSB4A50
access: public
definition: https://synbiohub.org/public/igem/BBa_B0042/1

BBa_P1002
type: Component
persistent identity: component2409818
display id: component2409818
version: 1
top level: pSB4A50
access: public
definition: https://synbiohub.org/public/igem/BBa_P1002/1

BBa_B0053
type: Component
persistent identity: component2409821
display id: component2409821
version: 1
top level: pSB4A50
access: public
definition: https://synbiohub.org/public/igem/BBa_B0053/1

BBa_B0053
type: SequenceAnnotation
persistent identity: annotation2409821
display id: annotation2409821
version: 1
top level: pSB4A50
location: https://synbiohub.org/public/igem/BBa_K1362095/annotation2409821/range2409821/1
component: BBa_B0053

https://synbiohub.org/public/igem/BBa_K1362095/annotation2409821/range2409821/1
type: Range
persistent identity: range2409821
display id: range2409821
version: 1
direction: direction/reverse
top level: pSB4A50
start: 3179
end: 3250
orientation: inline

BBa_K1362463
type: SequenceAnnotation
persistent identity: annotation2409842
display id: annotation2409842
version: 1
top level: pSB4A50
location: https://synbiohub.org/public/igem/BBa_K1362095/annotation2409842/range2409842/1
component: BBa_K1362463

https://synbiohub.org/public/igem/BBa_K1362095/annotation2409842/range2409842/1
type: Range
persistent identity: range2409842
display id: range2409842
version: 1
direction: direction/reverse
top level: pSB4A50
start: 3374
end: 3424
orientation: inline

BBa_B0055
type: SequenceAnnotation
persistent identity: annotation2409826
display id: annotation2409826
version: 1
top level: pSB4A50
location: https://synbiohub.org/public/igem/BBa_K1362095/annotation2409826/range2409826/1
component: BBa_B0055

https://synbiohub.org/public/igem/BBa_K1362095/annotation2409826/range2409826/1
type: Range
persistent identity: range2409826
display id: range2409826
version: 1
direction: direction/reverse
top level: pSB4A50
start: 3271
end: 3348
orientation: inline

BBa_B0042
type: SequenceAnnotation
persistent identity: annotation2409776
display id: annotation2409776
version: 1
top level: pSB4A50
location: https://synbiohub.org/public/igem/BBa_K1362095/annotation2409776/range2409776/1
component: BBa_B0042

https://synbiohub.org/public/igem/BBa_K1362095/annotation2409776/range2409776/1
type: Range
persistent identity: range2409776
display id: range2409776
version: 1
direction: direction/reverse
top level: pSB4A50
start: 35
end: 46
orientation: inline

BBa_G0003
type: SequenceAnnotation
persistent identity: annotation2409784
display id: annotation2409784
version: 1
top level: pSB4A50
location: https://synbiohub.org/public/igem/BBa_K1362095/annotation2409784/range2409784/1
component: BBa_G0003

https://synbiohub.org/public/igem/BBa_K1362095/annotation2409784/range2409784/1
type: Range
persistent identity: range2409784
display id: range2409784
version: 1
direction: direction/reverse
top level: pSB4A50
start: 177
end: 183
orientation: inline

BBa_B0043
type: SequenceAnnotation
persistent identity: annotation2409838
display id: annotation2409838
version: 1
top level: pSB4A50
location: https://synbiohub.org/public/igem/BBa_K1362095/annotation2409838/range2409838/1
component: BBa_B0043

https://synbiohub.org/public/igem/BBa_K1362095/annotation2409838/range2409838/1
type: Range
persistent identity: range2409838
display id: range2409838
version: 1
direction: direction/reverse
top level: pSB4A50
start: 3361
end: 3373
orientation: inline

BBa_G00100
type: SequenceAnnotation
persistent identity: annotation2409823
display id: annotation2409823
version: 1
top level: pSB4A50
location: https://synbiohub.org/public/igem/BBa_K1362095/annotation2409823/range2409823/1
component: BBa_G00100

https://synbiohub.org/public/igem/BBa_K1362095/annotation2409823/range2409823/1
type: Range
persistent identity: range2409823
display id: range2409823
version: 1
direction: direction/reverse
top level: pSB4A50
start: 3251
end: 3270
orientation: inline

BBa_G0002
type: SequenceAnnotation
persistent identity: annotation2409813
display id: annotation2409813
version: 1
top level: pSB4A50
location: https://synbiohub.org/public/igem/BBa_K1362095/annotation2409813/range2409813/1
component: BBa_G0002

https://synbiohub.org/public/igem/BBa_K1362095/annotation2409813/range2409813/1
type: Range
persistent identity: range2409813
display id: range2409813
version: 1
direction: direction/reverse
top level: pSB4A50
start: 2221
end: 2228
orientation: inline

BBa_G00102
type: SequenceAnnotation
persistent identity: annotation2409781
display id: annotation2409781
version: 1
top level: pSB4A50
location: https://synbiohub.org/public/igem/BBa_K1362095/annotation2409781/range2409781/1
component: BBa_G00102

https://synbiohub.org/public/igem/BBa_K1362095/annotation2409781/range2409781/1
type: Range
persistent identity: range2409781
display id: range2409781
version: 1
direction: direction/reverse
top level: pSB4A50
start: 116
end: 135
orientation: inline

BBa_B0044
type: SequenceAnnotation
persistent identity: annotation2409769
display id: annotation2409769
version: 1
top level: pSB4A50
location: https://synbiohub.org/public/igem/BBa_K1362095/annotation2409769/range2409769/1
component: BBa_B0044

https://synbiohub.org/public/igem/BBa_K1362095/annotation2409769/range2409769/1
type: Range
persistent identity: range2409769
display id: range2409769
version: 1
direction: direction/reverse
top level: pSB4A50
start: 22
end: 34
orientation: inline

BBa_B0042
type: SequenceAnnotation
persistent identity: annotation2409833
display id: annotation2409833
version: 1
top level: pSB4A50
location: https://synbiohub.org/public/igem/BBa_K1362095/annotation2409833/range2409833/1
component: BBa_B0042

https://synbiohub.org/public/igem/BBa_K1362095/annotation2409833/range2409833/1
type: Range
persistent identity: range2409833
display id: range2409833
version: 1
direction: direction/reverse
top level: pSB4A50
start: 3349
end: 3360
orientation: inline

BBa_G00001
type: SequenceAnnotation
persistent identity: annotation2409764
display id: annotation2409764
version: 1
top level: pSB4A50
location: https://synbiohub.org/public/igem/BBa_K1362095/annotation2409764/range2409764/1
component: BBa_G00001

https://synbiohub.org/public/igem/BBa_K1362095/annotation2409764/range2409764/1
type: Range
persistent identity: range2409764
display id: range2409764
version: 1
direction: direction/reverse
top level: pSB4A50
start: 1
end: 21
orientation: inline

BBa_P1002
type: SequenceAnnotation
persistent identity: annotation2409818
display id: annotation2409818
version: 1
top level: pSB4A50
location: https://synbiohub.org/public/igem/BBa_K1362095/annotation2409818/range2409818/1
component: BBa_P1002

https://synbiohub.org/public/igem/BBa_K1362095/annotation2409818/range2409818/1
type: Range
persistent identity: range2409818
display id: range2409818
version: 1
direction: direction/reverse
top level: pSB4A50
start: 2229
end: 3171
orientation: inline

BBa_I50042
type: SequenceAnnotation
persistent identity: annotation2409812
display id: annotation2409812
version: 1
top level: pSB4A50
location: https://synbiohub.org/public/igem/BBa_K1362095/annotation2409812/range2409812/1
component: BBa_I50042

https://synbiohub.org/public/igem/BBa_K1362095/annotation2409812/range2409812/1
type: Range
persistent identity: range2409812
display id: range2409812
version: 1
direction: direction/reverse
top level: pSB4A50
start: 184
end: 2220
orientation: inline

BBa_G0004
type: SequenceAnnotation
persistent identity: annotation2409819
display id: annotation2409819
version: 1
top level: pSB4A50
location: https://synbiohub.org/public/igem/BBa_K1362095/annotation2409819/range2409819/1
component: BBa_G0004

https://synbiohub.org/public/igem/BBa_K1362095/annotation2409819/range2409819/1
type: Range
persistent identity: range2409819
display id: range2409819
version: 1
direction: direction/reverse
top level: pSB4A50
start: 3172
end: 3178
orientation: inline

BBa_B0054
type: SequenceAnnotation
persistent identity: annotation2409779
display id: annotation2409779
version: 1
top level: pSB4A50
location: https://synbiohub.org/public/igem/BBa_K1362095/annotation2409779/range2409779/1
component: BBa_B0054

https://synbiohub.org/public/igem/BBa_K1362095/annotation2409779/range2409779/1
type: Range
persistent identity: range2409779
display id: range2409779
version: 1
direction: direction/reverse
top level: pSB4A50
start: 47
end: 115
orientation: inline

BBa_B0062
type: SequenceAnnotation
persistent identity: annotation2409783
display id: annotation2409783
version: 1
top level: pSB4A50
location: https://synbiohub.org/public/igem/BBa_K1362095/annotation2409783/range2409783/1
component: BBa_B0062

https://synbiohub.org/public/igem/BBa_K1362095/annotation2409783/range2409783/1
type: Range
persistent identity: range2409783
display id: range2409783
version: 1
direction: direction/reverse
top level: pSB4A50
start: 136
end: 176
orientation: inline

BBa_G00000
type: SequenceAnnotation
persistent identity: annotation2409847
display id: annotation2409847
version: 1
top level: pSB4A50
location: https://synbiohub.org/public/igem/BBa_K1362095/annotation2409847/range2409847/1
component: BBa_G00000

https://synbiohub.org/public/igem/BBa_K1362095/annotation2409847/range2409847/1
type: Range
persistent identity: range2409847
display id: range2409847
version: 1
direction: direction/reverse
top level: pSB4A50
start: 3425
end: 3446
orientation: inline

pSB6A1
type: ComponentDefinition
persistent identity: pSB6A1
display id: pSB6A1
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pBR322 converted to BioBrick vector (formally BBa_I739201)
creator: Stefan Luzi
created: 2007-10-22T11:00:00Z
modified: 2015-05-08T01:14:55Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _124_
m user id: 0
owner id: 2183
owning group id: 62
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: sadf
mutable notes: asdf
mutable provenance: sdf
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: RNAI transcript promoter -10 sequence
sequence annotation: rep pMB1
sequence annotation: rop
sequence annotation: L-strand Y effector site
sequence annotation: BioBrick prefix
sequence annotation: partial tetracycline resistance CDS (nonfunctional)
sequence annotation: beta-lactamase signal peptide
sequence annotation: RNAII transcript promoter -10 sequence
sequence annotation: RNAII transcript
sequence annotation: from pSC101
sequence annotation: RNAI transcript
sequence annotation: bla(P3) promoter -35 sequence
sequence annotation: bla(P3) promoter -10 sequence
sequence annotation: bla gene
sequence annotation: gamma-delta insertion sequence
sequence annotation: beta-lactamase mature peptide
sequence annotation: H-strand Y effector site
sequence annotation: beta-lactamase (bla gene)
sequence annotation: BioBrick suffix
sequence annotation: Repeat region 1
sequence annotation: RNAI transcript promoter -35 sequence
sequence annotation: RNAII transcript promoter -35 sequence
sequence: https://synbiohub.org/public/igem/pSB6A1_sequence/1

RNAI transcript promoter -10 sequence
type: SequenceAnnotation
persistent identity: annotation1969649
display id: annotation1969649
version: 1
top level: pSB6A1
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/pSB6A1/annotation1969649/range1969649/1

https://synbiohub.org/public/igem/pSB6A1/annotation1969649/range1969649/1
type: Range
persistent identity: range1969649
display id: range1969649
version: 1
direction: direction/forward
top level: pSB6A1
start: 2604
end: 2609
orientation: inline

rep pMB1
type: SequenceAnnotation
persistent identity: annotation1969645
display id: annotation1969645
version: 1
top level: pSB6A1
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB6A1/annotation1969645/range1969645/1

https://synbiohub.org/public/igem/pSB6A1/annotation1969645/range1969645/1
type: Range
persistent identity: range1969645
display id: range1969645
version: 1
direction: direction/reverse
top level: pSB6A1
start: 2161
end: 2775
orientation: inline

rop
type: SequenceAnnotation
persistent identity: annotation1969641
display id: annotation1969641
version: 1
top level: pSB6A1
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/pSB6A1/annotation1969641/range1969641/1

https://synbiohub.org/public/igem/pSB6A1/annotation1969641/range1969641/1
type: Range
persistent identity: range1969641
display id: range1969641
version: 1
direction: direction/forward
top level: pSB6A1
start: 1557
end: 1748
orientation: inline

L-strand Y effector site
type: SequenceAnnotation
persistent identity: annotation1969643
display id: annotation1969643
version: 1
top level: pSB6A1
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB6A1/annotation1969643/range1969643/1

https://synbiohub.org/public/igem/pSB6A1/annotation1969643/range1969643/1
type: Range
persistent identity: range1969643
display id: range1969643
version: 1
direction: direction/reverse
top level: pSB6A1
start: 1993
end: 2056
orientation: inline

BioBrick prefix
type: SequenceAnnotation
persistent identity: annotation1969658
display id: annotation1969658
version: 1
top level: pSB6A1
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/pSB6A1/annotation1969658/range1969658/1

https://synbiohub.org/public/igem/pSB6A1/annotation1969658/range1969658/1
type: Range
persistent identity: range1969658
display id: range1969658
version: 1
direction: direction/forward
top level: pSB6A1
start: 4001
end: 4022
orientation: inline

partial tetracycline resistance CDS (nonfunctional)
type: SequenceAnnotation
persistent identity: annotation1969638
display id: annotation1969638
version: 1
top level: pSB6A1
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/pSB6A1/annotation1969638/range1969638/1

https://synbiohub.org/public/igem/pSB6A1/annotation1969638/range1969638/1
type: Range
persistent identity: range1969638
display id: range1969638
version: 1
direction: direction/forward
top level: pSB6A1
start: 23
end: 918
orientation: inline

beta-lactamase signal peptide
type: SequenceAnnotation
persistent identity: annotation1969652
display id: annotation1969652
version: 1
top level: pSB6A1
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/pSB6A1/annotation1969652/range1969652/1

https://synbiohub.org/public/igem/pSB6A1/annotation1969652/range1969652/1
type: Range
persistent identity: range1969652
display id: range1969652
version: 1
direction: direction/reverse
top level: pSB6A1
start: 3727
end: 3795
orientation: inline

RNAII transcript promoter -10 sequence
type: SequenceAnnotation
persistent identity: annotation1969650
display id: annotation1969650
version: 1
top level: pSB6A1
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/pSB6A1/annotation1969650/range1969650/1

https://synbiohub.org/public/igem/pSB6A1/annotation1969650/range1969650/1
type: Range
persistent identity: range1969650
display id: range1969650
version: 1
direction: direction/reverse
top level: pSB6A1
start: 2738
end: 2743
orientation: inline

RNAII transcript
type: SequenceAnnotation
persistent identity: annotation1969644
display id: annotation1969644
version: 1
top level: pSB6A1
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB6A1/annotation1969644/range1969644/1

https://synbiohub.org/public/igem/pSB6A1/annotation1969644/range1969644/1
type: Range
persistent identity: range1969644
display id: range1969644
version: 1
direction: direction/reverse
top level: pSB6A1
start: 2176
end: 2728
orientation: inline

from pSC101
type: SequenceAnnotation
persistent identity: annotation1969640
display id: annotation1969640
version: 1
top level: pSB6A1
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB6A1/annotation1969640/range1969640/1

https://synbiohub.org/public/igem/pSB6A1/annotation1969640/range1969640/1
type: Range
persistent identity: range1969640
display id: range1969640
version: 1
direction: direction/forward
top level: pSB6A1
start: 1278
end: 1404
orientation: inline

RNAI transcript
type: SequenceAnnotation
persistent identity: annotation1969646
display id: annotation1969646
version: 1
top level: pSB6A1
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB6A1/annotation1969646/range1969646/1

https://synbiohub.org/public/igem/pSB6A1/annotation1969646/range1969646/1
type: Range
persistent identity: range1969646
display id: range1969646
version: 1
direction: direction/forward
top level: pSB6A1
start: 2618
end: 2725
orientation: inline

bla(P3) promoter -35 sequence
type: SequenceAnnotation
persistent identity: annotation1969656
display id: annotation1969656
version: 1
top level: pSB6A1
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/pSB6A1/annotation1969656/range1969656/1

https://synbiohub.org/public/igem/pSB6A1/annotation1969656/range1969656/1
type: Range
persistent identity: range1969656
display id: range1969656
version: 1
direction: direction/reverse
top level: pSB6A1
start: 3860
end: 3865
orientation: inline

bla(P3) promoter -10 sequence
type: SequenceAnnotation
persistent identity: annotation1969655
display id: annotation1969655
version: 1
top level: pSB6A1
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/pSB6A1/annotation1969655/range1969655/1

https://synbiohub.org/public/igem/pSB6A1/annotation1969655/range1969655/1
type: Range
persistent identity: range1969655
display id: range1969655
version: 1
direction: direction/reverse
top level: pSB6A1
start: 3839
end: 3844
orientation: inline

bla gene
type: SequenceAnnotation
persistent identity: annotation1969654
display id: annotation1969654
version: 1
top level: pSB6A1
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/pSB6A1/annotation1969654/range1969654/1

https://synbiohub.org/public/igem/pSB6A1/annotation1969654/range1969654/1
type: Range
persistent identity: range1969654
display id: range1969654
version: 1
direction: direction/reverse
top level: pSB6A1
start: 3803
end: 3807
orientation: inline

gamma-delta insertion sequence
type: SequenceAnnotation
persistent identity: annotation1969639
display id: annotation1969639
version: 1
top level: pSB6A1
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB6A1/annotation1969639/range1969639/1

https://synbiohub.org/public/igem/pSB6A1/annotation1969639/range1969639/1
type: Range
persistent identity: range1969639
display id: range1969639
version: 1
direction: direction/reverse
top level: pSB6A1
start: 1157
end: 1161
orientation: inline

beta-lactamase mature peptide
type: SequenceAnnotation
persistent identity: annotation1969653
display id: annotation1969653
version: 1
top level: pSB6A1
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/pSB6A1/annotation1969653/range1969653/1

https://synbiohub.org/public/igem/pSB6A1/annotation1969653/range1969653/1
type: Range
persistent identity: range1969653
display id: range1969653
version: 1
direction: direction/reverse
top level: pSB6A1
start: 2938
end: 3726
orientation: inline

H-strand Y effector site
type: SequenceAnnotation
persistent identity: annotation1969642
display id: annotation1969642
version: 1
top level: pSB6A1
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB6A1/annotation1969642/range1969642/1

https://synbiohub.org/public/igem/pSB6A1/annotation1969642/range1969642/1
type: Range
persistent identity: range1969642
display id: range1969642
version: 1
direction: direction/forward
top level: pSB6A1
start: 1653
end: 1809
orientation: inline

beta-lactamase (bla gene)
type: SequenceAnnotation
persistent identity: annotation1969647
display id: annotation1969647
version: 1
top level: pSB6A1
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/pSB6A1/annotation1969647/range1969647/1

https://synbiohub.org/public/igem/pSB6A1/annotation1969647/range1969647/1
type: Range
persistent identity: range1969647
display id: range1969647
version: 1
direction: direction/reverse
top level: pSB6A1
start: 2935
end: 3795
orientation: inline

BioBrick suffix
type: SequenceAnnotation
persistent identity: annotation1969637
display id: annotation1969637
version: 1
top level: pSB6A1
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/pSB6A1/annotation1969637/range1969637/1

https://synbiohub.org/public/igem/pSB6A1/annotation1969637/range1969637/1
type: Range
persistent identity: range1969637
display id: range1969637
version: 1
direction: direction/forward
top level: pSB6A1
start: 1
end: 21
orientation: inline

Repeat region 1
type: SequenceAnnotation
persistent identity: annotation1969657
display id: annotation1969657
version: 1
top level: pSB6A1
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB6A1/annotation1969657/range1969657/1

https://synbiohub.org/public/igem/pSB6A1/annotation1969657/range1969657/1
type: Range
persistent identity: range1969657
display id: range1969657
version: 1
direction: direction/forward
top level: pSB6A1
start: 2790
end: 4003
orientation: inline

RNAI transcript promoter -35 sequence
type: SequenceAnnotation
persistent identity: annotation1969648
display id: annotation1969648
version: 1
top level: pSB6A1
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/pSB6A1/annotation1969648/range1969648/1

https://synbiohub.org/public/igem/pSB6A1/annotation1969648/range1969648/1
type: Range
persistent identity: range1969648
display id: range1969648
version: 1
direction: direction/forward
top level: pSB6A1
start: 2582
end: 2587
orientation: inline

RNAII transcript promoter -35 sequence
type: SequenceAnnotation
persistent identity: annotation1969651
display id: annotation1969651
version: 1
top level: pSB6A1
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/pSB6A1/annotation1969651/range1969651/1

https://synbiohub.org/public/igem/pSB6A1/annotation1969651/range1969651/1
type: Range
persistent identity: range1969651
display id: range1969651
version: 1
direction: direction/reverse
top level: pSB6A1
start: 2759
end: 2764
orientation: inline

BBa_K106670
type: ComponentDefinition
persistent identity: BBa K106670
display id: BBa_K106670
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: AarI AD acceptor, pRS315, 8X LexAOPs Cyc1P, Adht1
creator: Andrew Horwitz
created: 2008-10-24T11:00:00Z
modified: 2015-05-08T01:08:59Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _226_
m user id: 0
owner id: 2659
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: This is an acceptor vector for AarI cloning with 8X Lex operators directly upstream of a Cyc1 promoter. The vector contains the AarI cloning cassette (AD sites, with Adh1 terminator). It is a pRS315 derived vector, for CEN ARS expression in cerevisiae (LEU2 marker).
mutable notes: LexA operator repeats were cloned into the PspOMI site at the 5' end of the Cyc1 promoter. The inner site was broken, thus the operator-promoter-AarI cassette fragment can be subcloned by PspOMI/SacI or Kpn1/SacI digestion into any other pRS series vector.
mutable provenance: This is a derivative of part BBa_K106014, the AarI AD acceptor vector (pRS315, Cyc1P, Adh1t).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: ATG start codon
sequence annotation: Cyc1 Promoter
sequence annotation: T3 promoter
sequence annotation: ampicillin resistance marker
sequence annotation: lac promoter
sequence annotation: pBR322 origin
sequence annotation: T7 promoter
sequence annotation: Adh1 terminator
sequence annotation: LEU2 Marker
sequence annotation: 8X LexA Operators
sequence annotation: f1 origin
sequence annotation: D overhang
sequence annotation: A overhang
sequence annotation: ampicillin resistance promoter
sequence annotation: Stop codons in all 3 frames
sequence annotation: lacZalpha reporter
sequence annotation: CEN ARS
sequence: https://synbiohub.org/public/igem/BBa_K106670_sequence/1

ATG start codon
type: SequenceAnnotation
persistent identity: annotation1999702
display id: annotation1999702
version: 1
top level: BBa_K106670
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K106670/annotation1999702/range1999702/1

https://synbiohub.org/public/igem/BBa_K106670/annotation1999702/range1999702/1
type: Range
persistent identity: range1999702
display id: range1999702
version: 1
direction: direction/forward
top level: BBa_K106670
start: 6547
end: 6549
orientation: inline

Cyc1 Promoter
type: SequenceAnnotation
persistent identity: annotation1990434
display id: annotation1990434
version: 1
top level: BBa_K106670
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K106670/annotation1990434/range1990434/1

https://synbiohub.org/public/igem/BBa_K106670/annotation1990434/range1990434/1
type: Range
persistent identity: range1990434
display id: range1990434
version: 1
direction: direction/forward
top level: BBa_K106670
start: 6300
end: 6556
orientation: inline

T3 promoter
type: SequenceAnnotation
persistent identity: annotation1999701
display id: annotation1999701
version: 1
top level: BBa_K106670
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K106670/annotation1999701/range1999701/1

https://synbiohub.org/public/igem/BBa_K106670/annotation1999701/range1999701/1
type: Range
persistent identity: range1999701
display id: range1999701
version: 1
direction: direction/forward
top level: BBa_K106670
start: 6095
end: 6114
orientation: inline

ampicillin resistance marker
type: SequenceAnnotation
persistent identity: annotation1990432
display id: annotation1990432
version: 1
top level: BBa_K106670
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K106670/annotation1990432/range1990432/1

https://synbiohub.org/public/igem/BBa_K106670/annotation1990432/range1990432/1
type: Range
persistent identity: range1990432
display id: range1990432
version: 1
direction: direction/forward
top level: BBa_K106670
start: 4053
end: 4911
orientation: inline

lac promoter
type: SequenceAnnotation
persistent identity: annotation1999700
display id: annotation1999700
version: 1
top level: BBa_K106670
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K106670/annotation1999700/range1999700/1

https://synbiohub.org/public/igem/BBa_K106670/annotation1999700/range1999700/1
type: Range
persistent identity: range1999700
display id: range1999700
version: 1
direction: direction/forward
top level: BBa_K106670
start: 5996
end: 6025
orientation: inline

pBR322 origin
type: SequenceAnnotation
persistent identity: annotation1999699
display id: annotation1999699
version: 1
top level: BBa_K106670
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K106670/annotation1999699/range1999699/1

https://synbiohub.org/public/igem/BBa_K106670/annotation1999699/range1999699/1
type: Range
persistent identity: range1999699
display id: range1999699
version: 1
direction: direction/forward
top level: BBa_K106670
start: 5068
end: 5687
orientation: inline

T7 promoter
type: SequenceAnnotation
persistent identity: annotation1999696
display id: annotation1999696
version: 1
top level: BBa_K106670
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K106670/annotation1999696/range1999696/1

https://synbiohub.org/public/igem/BBa_K106670/annotation1999696/range1999696/1
type: Range
persistent identity: range1999696
display id: range1999696
version: 1
direction: direction/reverse
top level: BBa_K106670
start: 224
end: 242
orientation: inline

Adh1 terminator
type: SequenceAnnotation
persistent identity: annotation1990437
display id: annotation1990437
version: 1
top level: BBa_K106670
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K106670/annotation1990437/range1990437/1

https://synbiohub.org/public/igem/BBa_K106670/annotation1990437/range1990437/1
type: Range
persistent identity: range1990437
display id: range1990437
version: 1
direction: direction/forward
top level: BBa_K106670
start: 23
end: 216
orientation: inline

LEU2 Marker
type: SequenceAnnotation
persistent identity: annotation1990438
display id: annotation1990438
version: 1
top level: BBa_K106670
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K106670/annotation1990438/range1990438/1

https://synbiohub.org/public/igem/BBa_K106670/annotation1990438/range1990438/1
type: Range
persistent identity: range1990438
display id: range1990438
version: 1
direction: direction/forward
top level: BBa_K106670
start: 1570
end: 2676
orientation: inline

8X LexA Operators
type: SequenceAnnotation
persistent identity: annotation1990433
display id: annotation1990433
version: 1
top level: BBa_K106670
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K106670/annotation1990433/range1990433/1

https://synbiohub.org/public/igem/BBa_K106670/annotation1990433/range1990433/1
type: Range
persistent identity: range1990433
display id: range1990433
version: 1
direction: direction/forward
top level: BBa_K106670
start: 6132
end: 6299
orientation: inline

f1 origin
type: SequenceAnnotation
persistent identity: annotation1999698
display id: annotation1999698
version: 1
top level: BBa_K106670
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K106670/annotation1999698/range1999698/1

https://synbiohub.org/public/igem/BBa_K106670/annotation1999698/range1999698/1
type: Range
persistent identity: range1999698
display id: range1999698
version: 1
direction: direction/forward
top level: BBa_K106670
start: 427
end: 733
orientation: inline

D overhang
type: SequenceAnnotation
persistent identity: annotation1999693
display id: annotation1999693
version: 1
top level: BBa_K106670
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K106670/annotation1999693/range1999693/1

https://synbiohub.org/public/igem/BBa_K106670/annotation1999693/range1999693/1
type: Range
persistent identity: range1999693
display id: range1999693
version: 1
direction: direction/forward
top level: BBa_K106670
start: 1
end: 4
orientation: inline

A overhang
type: SequenceAnnotation
persistent identity: annotation1999695
display id: annotation1999695
version: 1
top level: BBa_K106670
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K106670/annotation1999695/range1999695/1

https://synbiohub.org/public/igem/BBa_K106670/annotation1999695/range1999695/1
type: Range
persistent identity: range1999695
display id: range1999695
version: 1
direction: direction/forward
top level: BBa_K106670
start: 6556
end: 6559
orientation: inline

ampicillin resistance promoter
type: SequenceAnnotation
persistent identity: annotation1990435
display id: annotation1990435
version: 1
top level: BBa_K106670
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K106670/annotation1990435/range1990435/1

https://synbiohub.org/public/igem/BBa_K106670/annotation1990435/range1990435/1
type: Range
persistent identity: range1990435
display id: range1990435
version: 1
direction: direction/forward
top level: BBa_K106670
start: 3983
end: 4011
orientation: inline

Stop codons in all 3 frames
type: SequenceAnnotation
persistent identity: annotation1999694
display id: annotation1999694
version: 1
top level: BBa_K106670
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K106670/annotation1999694/range1999694/1

https://synbiohub.org/public/igem/BBa_K106670/annotation1999694/range1999694/1
type: Range
persistent identity: range1999694
display id: range1999694
version: 1
direction: direction/forward
top level: BBa_K106670
start: 11
end: 21
orientation: inline

lacZalpha reporter
type: SequenceAnnotation
persistent identity: annotation1999697
display id: annotation1999697
version: 1
top level: BBa_K106670
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K106670/annotation1999697/range1999697/1

https://synbiohub.org/public/igem/BBa_K106670/annotation1999697/range1999697/1
type: Range
persistent identity: range1999697
display id: range1999697
version: 1
direction: direction/forward
top level: BBa_K106670
start: 246
end: 405
orientation: inline

CEN ARS
type: SequenceAnnotation
persistent identity: annotation1990431
display id: annotation1990431
version: 1
top level: BBa_K106670
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K106670/annotation1990431/range1990431/1

https://synbiohub.org/public/igem/BBa_K106670/annotation1990431/range1990431/1
type: Range
persistent identity: range1990431
display id: range1990431
version: 1
direction: direction/reverse
top level: BBa_K106670
start: 3403
end: 3921
orientation: inline

pSB8A15FRT
type: ComponentDefinition
persistent identity: BBa K864020
display id: BBa_K864020
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Low copy BioBrick temperature sensitive standard vector
creator: Erik Lundin
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:37Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1124_
m user id: 0
owner id: 9827
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: pSB8A15 is a BioBrick standard vector with low copy pSC101ts replication origin (~5 copies per cell) and ampicillin antibiotic resistance marker, especially usable for Lambda Red recombineering in E coli. The pSC101ts is a temperature sensitive pSC101 repelication origin and propagates at 30???C but stop replicating at 42???C. The backbone sequence is based on pSB3T5, but the E coli His operon terminator BBa_B0053 has been replaced with the late terminator of the Salmonella phage P22.
mutable notes: The resistance cassette is terminated by a Lux bidirectional terminator. Adjacent to this terminator there is a designed primer binding site to where the forward primer for lambda red can be designed to bind to no matter what the insert in the cloning site is or what the resistance is. The resistance cassette is flanked by SalI and SacI restriction sites for easy switching of resistance in the backbone. The pSC101ts origin of replication is flaked by NheI and MluI restriction sites for easy switching of origin of replication.
mutable provenance: Based on BBa_K864017
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence: https://synbiohub.org/public/igem/BBa_K864020_sequence/1

BBa_J179001
type: ComponentDefinition
persistent identity: BBa J179001
display id: BBa_J179001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pBS2E: Bacillus subtilis vector, lacA integration, MLS resistance
creator: Jara Radeck
created: 2013-12-20T12:00:00Z
modified: 2015-08-31T04:08:35Z
discontinued: false
dominant: false
experience: experience/None
group u list: _865_
m user id: 0
owner id: 6378
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This part is an empty backbone vector for the usage in Bacillus subtilis. It integrates in the lacA locus and can be selected with erythromycin + lincomycin (MLS). It has a ampicillin resistance for cloning in E.coli.
mutable notes: Compatible with pBs1C and pBs4S in one strain.
mutable provenance: This backbone is a BioBricked version of the B. subtilis vector pAX01. This BioBrick is part of the LMU 2012 igem project [http://2012.igem.org/Team:LMU-Munich/Bacillus_BioBricks '''''Bacillus''B'''io'''B'''rick'''B'''ox] Reference: [http://www.ncbi.nlm.nih.gov/pubmed/11274134 H??rtl ''et al.'']
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: ori
sequence annotation: lacA back
sequence annotation: bla
sequence annotation: erm
sequence annotation: lacA front
sequence: https://synbiohub.org/public/igem/BBa_J179001_sequence/1

ori
type: SequenceAnnotation
persistent identity: annotation2371763
display id: annotation2371763
version: 1
top level: BBa_J179001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J179001/annotation2371763/range2371763/1

https://synbiohub.org/public/igem/BBa_J179001/annotation2371763/range2371763/1
type: Range
persistent identity: range2371763
display id: range2371763
version: 1
direction: direction/reverse
top level: BBa_J179001
start: 1758
end: 2381
orientation: inline

lacA back
type: SequenceAnnotation
persistent identity: annotation2371761
display id: annotation2371761
version: 1
top level: BBa_J179001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J179001/annotation2371761/range2371761/1

https://synbiohub.org/public/igem/BBa_J179001/annotation2371761/range2371761/1
type: Range
persistent identity: range2371761
display id: range2371761
version: 1
direction: direction/reverse
top level: BBa_J179001
start: 124
end: 531
orientation: inline

bla
type: SequenceAnnotation
persistent identity: annotation2371762
display id: annotation2371762
version: 1
top level: BBa_J179001
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J179001/annotation2371762/range2371762/1

https://synbiohub.org/public/igem/BBa_J179001/annotation2371762/range2371762/1
type: Range
persistent identity: range2371762
display id: range2371762
version: 1
direction: direction/forward
top level: BBa_J179001
start: 747
end: 1607
orientation: inline

erm
type: SequenceAnnotation
persistent identity: annotation2371765
display id: annotation2371765
version: 1
top level: BBa_J179001
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J179001/annotation2371765/range2371765/1

https://synbiohub.org/public/igem/BBa_J179001/annotation2371765/range2371765/1
type: Range
persistent identity: range2371765
display id: range2371765
version: 1
direction: direction/reverse
top level: BBa_J179001
start: 4891
end: 5628
orientation: inline

lacA front
type: SequenceAnnotation
persistent identity: annotation2371764
display id: annotation2371764
version: 1
top level: BBa_J179001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J179001/annotation2371764/range2371764/1

https://synbiohub.org/public/igem/BBa_J179001/annotation2371764/range2371764/1
type: Range
persistent identity: range2371764
display id: range2371764
version: 1
direction: direction/reverse
top level: BBa_J179001
start: 4078
end: 4593
orientation: inline

BBa_K106697
type: ComponentDefinition
persistent identity: BBa K106697
display id: BBa_K106697
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: AarI AD acceptor vector (pRS315, Cyc1P, Adh1t-8XLexA Ops)
creator: Andrew Horwitz
created: 2008-10-28T12:00:00Z
modified: 2015-05-08T01:09:00Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _226_
m user id: 0
owner id: 2659
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: This is a variant of the AarI AD acceptor vector (pRS315, Cyc1P, Adh1t), with the addition of 8X LexA operator sites directly downstream of the Adht1 terminator.
mutable notes: None.
mutable provenance: Variant of the AarI AD acceptor vector (pRS315, Cyc1P, Adh1t)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: A overhang
sequence annotation: 8X LexA Operators
sequence annotation: ampicillin resistance marker
sequence annotation: pBR322 origin
sequence annotation: lac promoter
sequence annotation: Stop codons in all 3 frames
sequence annotation: T7 promoter
sequence annotation: D overhang
sequence annotation: LEU2 marker
sequence annotation: CEN ARS
sequence annotation: T3 promoter
sequence annotation: f1 origin
sequence annotation: Start codon
sequence annotation: Adh1 terminator
sequence annotation: lacZalpha reporter
sequence annotation: ampicillin resistance promoter
sequence annotation: Cyc1 Promoter
sequence: https://synbiohub.org/public/igem/BBa_K106697_sequence/1

A overhang
type: SequenceAnnotation
persistent identity: annotation1999737
display id: annotation1999737
version: 1
top level: BBa_K106697
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K106697/annotation1999737/range1999737/1

https://synbiohub.org/public/igem/BBa_K106697/annotation1999737/range1999737/1
type: Range
persistent identity: range1999737
display id: range1999737
version: 1
direction: direction/forward
top level: BBa_K106697
start: 6556
end: 6559
orientation: inline

8X LexA Operators
type: SequenceAnnotation
persistent identity: annotation1995079
display id: annotation1995079
version: 1
top level: BBa_K106697
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K106697/annotation1995079/range1995079/1

https://synbiohub.org/public/igem/BBa_K106697/annotation1995079/range1995079/1
type: Range
persistent identity: range1995079
display id: range1995079
version: 1
direction: direction/forward
top level: BBa_K106697
start: 210
end: 377
orientation: inline

ampicillin resistance marker
type: SequenceAnnotation
persistent identity: annotation1995018
display id: annotation1995018
version: 1
top level: BBa_K106697
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K106697/annotation1995018/range1995018/1

https://synbiohub.org/public/igem/BBa_K106697/annotation1995018/range1995018/1
type: Range
persistent identity: range1995018
display id: range1995018
version: 1
direction: direction/forward
top level: BBa_K106697
start: 4215
end: 5075
orientation: inline

pBR322 origin
type: SequenceAnnotation
persistent identity: annotation1999732
display id: annotation1999732
version: 1
top level: BBa_K106697
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K106697/annotation1999732/range1999732/1

https://synbiohub.org/public/igem/BBa_K106697/annotation1999732/range1999732/1
type: Range
persistent identity: range1999732
display id: range1999732
version: 1
direction: direction/forward
top level: BBa_K106697
start: 5230
end: 5849
orientation: inline

lac promoter
type: SequenceAnnotation
persistent identity: annotation1999733
display id: annotation1999733
version: 1
top level: BBa_K106697
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K106697/annotation1999733/range1999733/1

https://synbiohub.org/public/igem/BBa_K106697/annotation1999733/range1999733/1
type: Range
persistent identity: range1999733
display id: range1999733
version: 1
direction: direction/forward
top level: BBa_K106697
start: 6158
end: 6187
orientation: inline

Stop codons in all 3 frames
type: SequenceAnnotation
persistent identity: annotation1999735
display id: annotation1999735
version: 1
top level: BBa_K106697
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K106697/annotation1999735/range1999735/1

https://synbiohub.org/public/igem/BBa_K106697/annotation1999735/range1999735/1
type: Range
persistent identity: range1999735
display id: range1999735
version: 1
direction: direction/forward
top level: BBa_K106697
start: 11
end: 21
orientation: inline

T7 promoter
type: SequenceAnnotation
persistent identity: annotation1999728
display id: annotation1999728
version: 1
top level: BBa_K106697
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K106697/annotation1999728/range1999728/1

https://synbiohub.org/public/igem/BBa_K106697/annotation1999728/range1999728/1
type: Range
persistent identity: range1999728
display id: range1999728
version: 1
direction: direction/reverse
top level: BBa_K106697
start: 386
end: 404
orientation: inline

D overhang
type: SequenceAnnotation
persistent identity: annotation1995020
display id: annotation1995020
version: 1
top level: BBa_K106697
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K106697/annotation1995020/range1995020/1

https://synbiohub.org/public/igem/BBa_K106697/annotation1995020/range1995020/1
type: Range
persistent identity: range1995020
display id: range1995020
version: 1
direction: direction/forward
top level: BBa_K106697
start: 1
end: 4
orientation: inline

LEU2 marker
type: SequenceAnnotation
persistent identity: annotation1995101
display id: annotation1995101
version: 1
top level: BBa_K106697
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K106697/annotation1995101/range1995101/1

https://synbiohub.org/public/igem/BBa_K106697/annotation1995101/range1995101/1
type: Range
persistent identity: range1995101
display id: range1995101
version: 1
direction: direction/forward
top level: BBa_K106697
start: 1732
end: 2838
orientation: inline

CEN ARS
type: SequenceAnnotation
persistent identity: annotation1995017
display id: annotation1995017
version: 1
top level: BBa_K106697
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K106697/annotation1995017/range1995017/1

https://synbiohub.org/public/igem/BBa_K106697/annotation1995017/range1995017/1
type: Range
persistent identity: range1995017
display id: range1995017
version: 1
direction: direction/reverse
top level: BBa_K106697
start: 3565
end: 4083
orientation: inline

T3 promoter
type: SequenceAnnotation
persistent identity: annotation1999734
display id: annotation1999734
version: 1
top level: BBa_K106697
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K106697/annotation1999734/range1999734/1

https://synbiohub.org/public/igem/BBa_K106697/annotation1999734/range1999734/1
type: Range
persistent identity: range1999734
display id: range1999734
version: 1
direction: direction/forward
top level: BBa_K106697
start: 6257
end: 6276
orientation: inline

f1 origin
type: SequenceAnnotation
persistent identity: annotation1999730
display id: annotation1999730
version: 1
top level: BBa_K106697
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K106697/annotation1999730/range1999730/1

https://synbiohub.org/public/igem/BBa_K106697/annotation1999730/range1999730/1
type: Range
persistent identity: range1999730
display id: range1999730
version: 1
direction: direction/forward
top level: BBa_K106697
start: 589
end: 895
orientation: inline

Start codon
type: SequenceAnnotation
persistent identity: annotation1999736
display id: annotation1999736
version: 1
top level: BBa_K106697
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K106697/annotation1999736/range1999736/1

https://synbiohub.org/public/igem/BBa_K106697/annotation1999736/range1999736/1
type: Range
persistent identity: range1999736
display id: range1999736
version: 1
direction: direction/forward
top level: BBa_K106697
start: 6547
end: 6549
orientation: inline

Adh1 terminator
type: SequenceAnnotation
persistent identity: annotation1995021
display id: annotation1995021
version: 1
top level: BBa_K106697
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K106697/annotation1995021/range1995021/1

https://synbiohub.org/public/igem/BBa_K106697/annotation1995021/range1995021/1
type: Range
persistent identity: range1995021
display id: range1995021
version: 1
direction: direction/forward
top level: BBa_K106697
start: 30
end: 209
orientation: inline

lacZalpha reporter
type: SequenceAnnotation
persistent identity: annotation1999729
display id: annotation1999729
version: 1
top level: BBa_K106697
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K106697/annotation1999729/range1999729/1

https://synbiohub.org/public/igem/BBa_K106697/annotation1999729/range1999729/1
type: Range
persistent identity: range1999729
display id: range1999729
version: 1
direction: direction/forward
top level: BBa_K106697
start: 408
end: 567
orientation: inline

ampicillin resistance promoter
type: SequenceAnnotation
persistent identity: annotation1999731
display id: annotation1999731
version: 1
top level: BBa_K106697
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K106697/annotation1999731/range1999731/1

https://synbiohub.org/public/igem/BBa_K106697/annotation1999731/range1999731/1
type: Range
persistent identity: range1999731
display id: range1999731
version: 1
direction: direction/forward
top level: BBa_K106697
start: 4145
end: 4173
orientation: inline

Cyc1 Promoter
type: SequenceAnnotation
persistent identity: annotation1995019
display id: annotation1995019
version: 1
top level: BBa_K106697
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K106697/annotation1995019/range1995019/1

https://synbiohub.org/public/igem/BBa_K106697/annotation1995019/range1995019/1
type: Range
persistent identity: range1995019
display id: range1995019
version: 1
direction: direction/forward
top level: BBa_K106697
start: 6294
end: 6556
orientation: inline

pSB8C15FRT
type: ComponentDefinition
persistent identity: BBa K864021
display id: BBa_K864021
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Low copy BioBrick temperature sensitive standard vector
creator: Erik Lundin
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:37Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1124_
m user id: 0
owner id: 9827
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: pSB8A15 is a BioBrick standard vector with low copy pSC101ts replication origin (~5 copies per cell) and ampicillin antibiotic resistance marker, especially usable for Lambda Red recombineering in E coli. The pSC101ts is a temperature sensitive pSC101 repelication origin and propagates at 30???C but stop replicating at 42???C. The backbone sequence is based on pSB3T5, but the E coli His operon terminator BBa_B0053 has been replaced with the late terminator of the Salmonella phage P22
mutable notes: The resistance cassette is terminated by a Lux bidirectional terminator. Adjacent to this terminator there is a designed primer binding site to where the forward primer for lambda red can be designed to bind to no matter what the insert in the cloning site is or what the resistance is. The resistance cassette is flanked by SalI and SacI restriction sites for easy switching of resistance in the backbone. The pSC101ts origin of replication is flaked by NheI and MluI restriction sites for easy switching of origin of replication.
mutable provenance: Based on BBa_K864017
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence: https://synbiohub.org/public/igem/BBa_K864021_sequence/1

BBa_K864008
type: ComponentDefinition
persistent identity: BBa K864008
display id: BBa_K864008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Low copy BioBrick standard vector with LacI
creator: Erik Lundin
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:37Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1124_
m user id: 0
owner id: 9827
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: pSB4A15 is a BioBrick standard vector with low copy pSC101 replication origin (~5 copies per cell) and ampicillin antibiotic resistance marker, especially usable for Lambda Red recombineering in E coli. The backbone sequence is based on pSB3T5, but the E coli His operon terminator BBa_B0053 has been replaced with the late terminator of the Salmonella phage P22. This vector carries a LacIq cassette on the backbone for tight repression of any lac promoter cloned into the cloning site.
mutable notes: The resistance cassette is terminated by a Lux bidirectional terminator. Adjacent to this terminator there is a designed primer binding site to where the forward primer for lambda red can be designed to bind to no matter what the insert in the cloning site is or what the resistance is. The resistance cassette is flanked by SalI and SacI restriction sites for easy switching of resistance in the backbone. The pSC101 origin of replication is flaked by NheI and MluI restriction sites for easy switching of origin of replication.
mutable provenance: Based on BBa_K864000
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence: https://synbiohub.org/public/igem/BBa_K864008_sequence/1

BBa_K864000
type: ComponentDefinition
persistent identity: BBa K864000
display id: BBa_K864000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Low copy BioBrick standard vector
creator: Erik Lundin
created: 2012-09-22T11:00:00Z
modified: 2015-05-08T01:13:37Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1124_
m user id: 0
owner id: 9827
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: pSB4A15 is a BioBrick standard vector with low copy pSC101 replication origin (~5 copies per cell) and ??? antibiotic resistance marker, especially usable for Lambda Red recombineering in E coli. The backbone sequence is based on pSB3T5, but the E coli His operon terminator BBa_B0053 has been replaced with the late terminator of the Salmonella phage P22.
mutable notes: The resistance cassette is terminated by a Lux bidirectional terminator. Adjacent to this terminator there is a designed primer binding site to where the forward primer for lambda red can be designed to bind to no matter what the insert in the cloning site is or what the resistance is. The resistance cassette is flanked by SalI and SacI restriction sites for easy switching of resistance in the backbone. The pSC101 origin of replication is flaked by NheI and MluI restriction sites for easy switching of origin of replication.
mutable provenance: Based on pSB3T5
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: VR
sequence annotation: -10
sequence annotation: tonB terminator
sequence annotation: pSC101 ori
sequence annotation: Tandem repeat
sequence annotation: ampR BBa_P1002
sequence annotation: Mutated A>T to remove SpeI
sequence annotation: Lambda Red forward primer
sequence annotation: -35
sequence annotation: Lambda Red reverse primer
sequence annotation: Repeat "5"
sequence annotation: Lux terminator
sequence annotation: RBS
sequence annotation: T3 terminator
sequence annotation: Repeat "4"
sequence annotation: rrnC terminator
sequence annotation: repA
sequence annotation: VF2
sequence: https://synbiohub.org/public/igem/BBa_K864000_sequence/1

VR
type: SequenceAnnotation
persistent identity: annotation2198033
display id: annotation2198033
version: 1
top level: BBa_K864000
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K864000/annotation2198033/range2198033/1

https://synbiohub.org/public/igem/BBa_K864000/annotation2198033/range2198033/1
type: Range
persistent identity: range2198033
display id: range2198033
version: 1
direction: direction/reverse
top level: BBa_K864000
start: 116
end: 135
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2198007
display id: annotation2198007
version: 1
top level: BBa_K864000
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K864000/annotation2198007/range2198007/1

https://synbiohub.org/public/igem/BBa_K864000/annotation2198007/range2198007/1
type: Range
persistent identity: range2198007
display id: range2198007
version: 1
direction: direction/reverse
top level: BBa_K864000
start: 3387
end: 3392
orientation: inline

tonB terminator
type: SequenceAnnotation
persistent identity: annotation2198074
display id: annotation2198074
version: 1
top level: BBa_K864000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864000/annotation2198074/range2198074/1

https://synbiohub.org/public/igem/BBa_K864000/annotation2198074/range2198074/1
type: Range
persistent identity: range2198074
display id: range2198074
version: 1
direction: direction/forward
top level: BBa_K864000
start: 47
end: 115
orientation: inline

pSC101 ori
type: SequenceAnnotation
persistent identity: annotation2198057
display id: annotation2198057
version: 1
top level: BBa_K864000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864000/annotation2198057/range2198057/1

https://synbiohub.org/public/igem/BBa_K864000/annotation2198057/range2198057/1
type: Range
persistent identity: range2198057
display id: range2198057
version: 1
direction: direction/forward
top level: BBa_K864000
start: 190
end: 2405
orientation: inline

Tandem repeat
type: SequenceAnnotation
persistent identity: annotation2198036
display id: annotation2198036
version: 1
top level: BBa_K864000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864000/annotation2198036/range2198036/1

https://synbiohub.org/public/igem/BBa_K864000/annotation2198036/range2198036/1
type: Range
persistent identity: range2198036
display id: range2198036
version: 1
direction: direction/forward
top level: BBa_K864000
start: 790
end: 809
orientation: inline

ampR BBa_P1002
type: SequenceAnnotation
persistent identity: annotation2198012
display id: annotation2198012
version: 1
top level: BBa_K864000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864000/annotation2198012/range2198012/1

https://synbiohub.org/public/igem/BBa_K864000/annotation2198012/range2198012/1
type: Range
persistent identity: range2198012
display id: range2198012
version: 1
direction: direction/reverse
top level: BBa_K864000
start: 2509
end: 3369
orientation: inline

Mutated A>T to remove SpeI
type: SequenceAnnotation
persistent identity: annotation2198029
display id: annotation2198029
version: 1
top level: BBa_K864000
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K864000/annotation2198029/range2198029/1

https://synbiohub.org/public/igem/BBa_K864000/annotation2198029/range2198029/1
type: Range
persistent identity: range2198029
display id: range2198029
version: 1
direction: direction/forward
top level: BBa_K864000
start: 867
end: 867
orientation: inline

Lambda Red forward primer
type: SequenceAnnotation
persistent identity: annotation2198030
display id: annotation2198030
version: 1
top level: BBa_K864000
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K864000/annotation2198030/range2198030/1

https://synbiohub.org/public/igem/BBa_K864000/annotation2198030/range2198030/1
type: Range
persistent identity: range2198030
display id: range2198030
version: 1
direction: direction/forward
top level: BBa_K864000
start: 2433
end: 2450
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2198009
display id: annotation2198009
version: 1
top level: BBa_K864000
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K864000/annotation2198009/range2198009/1

https://synbiohub.org/public/igem/BBa_K864000/annotation2198009/range2198009/1
type: Range
persistent identity: range2198009
display id: range2198009
version: 1
direction: direction/forward
top level: BBa_K864000
start: 881
end: 886
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2198006
display id: annotation2198006
version: 1
top level: BBa_K864000
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K864000/annotation2198006/range2198006/1

https://synbiohub.org/public/igem/BBa_K864000/annotation2198006/range2198006/1
type: Range
persistent identity: range2198006
display id: range2198006
version: 1
direction: direction/forward
top level: BBa_K864000
start: 903
end: 908
orientation: inline

Tandem repeat
type: SequenceAnnotation
persistent identity: annotation2198037
display id: annotation2198037
version: 1
top level: BBa_K864000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864000/annotation2198037/range2198037/1

https://synbiohub.org/public/igem/BBa_K864000/annotation2198037/range2198037/1
type: Range
persistent identity: range2198037
display id: range2198037
version: 1
direction: direction/forward
top level: BBa_K864000
start: 767
end: 788
orientation: inline

Tandem repeat
type: SequenceAnnotation
persistent identity: annotation2198039
display id: annotation2198039
version: 1
top level: BBa_K864000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864000/annotation2198039/range2198039/1

https://synbiohub.org/public/igem/BBa_K864000/annotation2198039/range2198039/1
type: Range
persistent identity: range2198039
display id: range2198039
version: 1
direction: direction/forward
top level: BBa_K864000
start: 820
end: 841
orientation: inline

Lambda Red reverse primer
type: SequenceAnnotation
persistent identity: annotation2198031
display id: annotation2198031
version: 1
top level: BBa_K864000
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K864000/annotation2198031/range2198031/1

https://synbiohub.org/public/igem/BBa_K864000/annotation2198031/range2198031/1
type: Range
persistent identity: range2198031
display id: range2198031
version: 1
direction: direction/reverse
top level: BBa_K864000
start: 18
end: 38
orientation: inline

Repeat "5"
type: SequenceAnnotation
persistent identity: annotation2198056
display id: annotation2198056
version: 1
top level: BBa_K864000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864000/annotation2198056/range2198056/1

https://synbiohub.org/public/igem/BBa_K864000/annotation2198056/range2198056/1
type: Range
persistent identity: range2198056
display id: range2198056
version: 1
direction: direction/forward
top level: BBa_K864000
start: 891
end: 912
orientation: inline

Lux terminator
type: SequenceAnnotation
persistent identity: annotation2198075
display id: annotation2198075
version: 1
top level: BBa_K864000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864000/annotation2198075/range2198075/1

https://synbiohub.org/public/igem/BBa_K864000/annotation2198075/range2198075/1
type: Range
persistent identity: range2198075
display id: range2198075
version: 1
direction: direction/reverse
top level: BBa_K864000
start: 2454
end: 2499
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2198034
display id: annotation2198034
version: 1
top level: BBa_K864000
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K864000/annotation2198034/range2198034/1

https://synbiohub.org/public/igem/BBa_K864000/annotation2198034/range2198034/1
type: Range
persistent identity: range2198034
display id: range2198034
version: 1
direction: direction/forward
top level: BBa_K864000
start: 3374
end: 3378
orientation: inline

T3 terminator
type: SequenceAnnotation
persistent identity: annotation2198076
display id: annotation2198076
version: 1
top level: BBa_K864000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864000/annotation2198076/range2198076/1

https://synbiohub.org/public/igem/BBa_K864000/annotation2198076/range2198076/1
type: Range
persistent identity: range2198076
display id: range2198076
version: 1
direction: direction/forward
top level: BBa_K864000
start: 3515
end: 3592
orientation: inline

Repeat "4"
type: SequenceAnnotation
persistent identity: annotation2198038
display id: annotation2198038
version: 1
top level: BBa_K864000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864000/annotation2198038/range2198038/1

https://synbiohub.org/public/igem/BBa_K864000/annotation2198038/range2198038/1
type: Range
persistent identity: range2198038
display id: range2198038
version: 1
direction: direction/forward
top level: BBa_K864000
start: 862
end: 880
orientation: inline

rrnC terminator
type: SequenceAnnotation
persistent identity: annotation2198093
display id: annotation2198093
version: 1
top level: BBa_K864000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864000/annotation2198093/range2198093/1

https://synbiohub.org/public/igem/BBa_K864000/annotation2198093/range2198093/1
type: Range
persistent identity: range2198093
display id: range2198093
version: 1
direction: direction/forward
top level: BBa_K864000
start: 136
end: 175
orientation: inline

repA
type: SequenceAnnotation
persistent identity: annotation2198011
display id: annotation2198011
version: 1
top level: BBa_K864000
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K864000/annotation2198011/range2198011/1

https://synbiohub.org/public/igem/BBa_K864000/annotation2198011/range2198011/1
type: Range
persistent identity: range2198011
display id: range2198011
version: 1
direction: direction/forward
top level: BBa_K864000
start: 939
end: 1889
orientation: inline

VF2
type: SequenceAnnotation
persistent identity: annotation2198032
display id: annotation2198032
version: 1
top level: BBa_K864000
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K864000/annotation2198032/range2198032/1

https://synbiohub.org/public/igem/BBa_K864000/annotation2198032/range2198032/1
type: Range
persistent identity: range2198032
display id: range2198032
version: 1
direction: direction/forward
top level: BBa_K864000
start: 3495
end: 3514
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2198010
display id: annotation2198010
version: 1
top level: BBa_K864000
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K864000/annotation2198010/range2198010/1

https://synbiohub.org/public/igem/BBa_K864000/annotation2198010/range2198010/1
type: Range
persistent identity: range2198010
display id: range2198010
version: 1
direction: direction/reverse
top level: BBa_K864000
start: 3413
end: 3418
orientation: inline

BBa_K864013
type: ComponentDefinition
persistent identity: BBa K864013
display id: BBa_K864013
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Low copy BioBrick standard vector with LacI
creator: Erik Lundin
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:37Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1124_
m user id: 0
owner id: 9827
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: pSB4C15Iq(FRT) is a BioBrick standard vector with low copy pSC101 replication origin (~5 copies per cell) and chloramphenicol antibiotic resistance marker, especially usable for Lambda Red recombineering in E coli. The backbone sequence is based on pSB3T5, but the E coli His operon terminator BBa_B0053 has been replaced with the late terminator of the Salmonella phage P22. This vector carries a LacIq cassette on the backbone for tight repression of any lac promoter cloned into the cloning site.
mutable notes: The resistance cassette is terminated by a Lux bidirectional terminator. Adjacent to this terminator there is a designed primer binding site to where the forward primer for lambda red can be designed to bind to no matter what the insert in the cloning site is or what the resistance is. The resistance cassette is flanked by SalI and SacI restriction sites for easy switching of resistance in the backbone.
mutable provenance: Based on BBa_K864005
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence: https://synbiohub.org/public/igem/BBa_K864013_sequence/1

pSB2K4
type: ComponentDefinition
persistent identity: pSB2K4
display id: pSB2K4
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pSB2K3-derived BioBrick plasmid free of many restriction sites
creator: Austin Che, Leon Chan and Sri Kosuri
created: 2006-01-19T12:00:00Z
modified: 2015-05-08T01:14:55Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _41_6_
m user id: 0
owner id: 22
owning group id: 41
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Derived from pSB2K3 with many restriction enzyme sites removed by site-directed mutagenesis (~2 dozen mutations). Plasmid is useful when there is a need to cut with a variety of different restriction enzymes. Most symmetric cutters have been removed, and some non-symmetric cutters have also including AarI, AcuI, BbsI, BbvCI, BciVI, BsaI, BsmBI, EarI, N.BstNBI, and SgrAI. Works in exactly same way as pSB2K3. See <bb_part>pSB2K3</bb_part> for more information. Note that the VF2 site has a single base mutation in the middle of it. However, VF2 has been used successfully as a primer for PCR with this plasmid.
mutable provenance: pSB2K3
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: repE binding site
sequence annotation: oriS
sequence annotation: Possible -35
sequence annotation: BioBrick prefix
sequence annotation: Possible -10
sequence annotation: Predicted transcription start
sequence annotation: Stem loop
sequence annotation: T->G
sequence annotation: end of terminator
sequence annotation: f1 ori
sequence annotation: Verification reverse
sequence annotation: T->C
sequence annotation: repE
sequence annotation: C->T
sequence annotation: VF2 (not an exact match, but works)
sequence annotation: nnpp promoter 2 (0.96)'
sequence annotation: Low GC region
sequence annotation: Maybe A or G
sequence annotation: repL
sequence annotation: incC
sequence annotation: BioBrick suffix
sequence annotation: G->A
sequence annotation: G->C
sequence annotation: nnpp promoter 1 (.92)'
sequence annotation: Predicted -35
sequence annotation: E. coli his operon terminator
sequence annotation: nnpp promoter 3 (0.95)'
sequence annotation: T->A mutation
sequence annotation: A->G mutation
sequence annotation: A->C
sequence annotation: Predicted -10
sequence annotation: C->A

repE binding site
type: SequenceAnnotation
persistent identity: annotation1974325
display id: annotation1974325
version: 1
top level: pSB2K4
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974325/range1974325/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974325/range1974325/1
type: Range
persistent identity: range1974325
display id: range1974325
version: 1
direction: direction/forward
top level: pSB2K4
start: 1527
end: 1537
orientation: inline

oriS
type: SequenceAnnotation
persistent identity: annotation1974328
display id: annotation1974328
version: 1
top level: pSB2K4
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974328/range1974328/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974328/range1974328/1
type: Range
persistent identity: range1974328
display id: range1974328
version: 1
direction: direction/reverse
top level: pSB2K4
start: 1490
end: 1706
orientation: inline

Possible -35
type: SequenceAnnotation
persistent identity: annotation1974323
display id: annotation1974323
version: 1
top level: pSB2K4
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974323/range1974323/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974323/range1974323/1
type: Range
persistent identity: range1974323
display id: range1974323
version: 1
direction: direction/reverse
top level: pSB2K4
start: 1477
end: 1482
orientation: inline

BioBrick prefix
type: SequenceAnnotation
persistent identity: annotation1974355
display id: annotation1974355
version: 1
top level: pSB2K4
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974355/range1974355/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974355/range1974355/1
type: Range
persistent identity: range1974355
display id: range1974355
version: 1
direction: direction/forward
top level: pSB2K4
start: 4446
end: 4467
orientation: inline

Possible -10
type: SequenceAnnotation
persistent identity: annotation1974320
display id: annotation1974320
version: 1
top level: pSB2K4
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974320/range1974320/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974320/range1974320/1
type: Range
persistent identity: range1974320
display id: range1974320
version: 1
direction: direction/reverse
top level: pSB2K4
start: 1455
end: 1463
orientation: inline

Predicted transcription start
type: SequenceAnnotation
persistent identity: annotation1974318
display id: annotation1974318
version: 1
top level: pSB2K4
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974318/range1974318/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974318/range1974318/1
type: Range
persistent identity: range1974318
display id: range1974318
version: 1
direction: direction/reverse
top level: pSB2K4
start: 1447
end: 1447
orientation: inline

Stem loop
type: SequenceAnnotation
persistent identity: annotation1974354
display id: annotation1974354
version: 1
top level: pSB2K4
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974354/range1974354/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974354/range1974354/1
type: Range
persistent identity: range1974354
display id: range1974354
version: 1
direction: direction/forward
top level: pSB2K4
start: 4402
end: 4421
orientation: inline

T->G
type: SequenceAnnotation
persistent identity: annotation1974334
display id: annotation1974334
version: 1
top level: pSB2K4
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974334/range1974334/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974334/range1974334/1
type: Range
persistent identity: range1974334
display id: range1974334
version: 1
direction: direction/forward
top level: pSB2K4
start: 2699
end: 2699
orientation: inline

end of terminator
type: SequenceAnnotation
persistent identity: annotation1974353
display id: annotation1974353
version: 1
top level: pSB2K4
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974353/range1974353/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974353/range1974353/1
type: Range
persistent identity: range1974353
display id: range1974353
version: 1
direction: direction/forward
top level: pSB2K4
start: 4389
end: 4392
orientation: inline

Possible -35
type: SequenceAnnotation
persistent identity: annotation1974315
display id: annotation1974315
version: 1
top level: pSB2K4
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974315/range1974315/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974315/range1974315/1
type: Range
persistent identity: range1974315
display id: range1974315
version: 1
direction: direction/forward
top level: pSB2K4
start: 1426
end: 1431
orientation: inline

f1 ori
type: SequenceAnnotation
persistent identity: annotation1974297
display id: annotation1974297
version: 1
top level: pSB2K4
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974297/range1974297/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974297/range1974297/1
type: Range
persistent identity: range1974297
display id: range1974297
version: 1
direction: direction/reverse
top level: pSB2K4
start: 269
end: 317
orientation: inline

Verification reverse
type: SequenceAnnotation
persistent identity: annotation1974296
display id: annotation1974296
version: 1
top level: pSB2K4
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974296/range1974296/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974296/range1974296/1
type: Range
persistent identity: range1974296
display id: range1974296
version: 1
direction: direction/reverse
top level: pSB2K4
start: 184
end: 203
orientation: inline

T->C
type: SequenceAnnotation
persistent identity: annotation1974338
display id: annotation1974338
version: 1
top level: pSB2K4
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974338/range1974338/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974338/range1974338/1
type: Range
persistent identity: range1974338
display id: range1974338
version: 1
direction: direction/forward
top level: pSB2K4
start: 3251
end: 3251
orientation: inline

T->G
type: SequenceAnnotation
persistent identity: annotation1974349
display id: annotation1974349
version: 1
top level: pSB2K4
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974349/range1974349/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974349/range1974349/1
type: Range
persistent identity: range1974349
display id: range1974349
version: 1
direction: direction/forward
top level: pSB2K4
start: 3903
end: 3903
orientation: inline

repE binding site
type: SequenceAnnotation
persistent identity: annotation1974304
display id: annotation1974304
version: 1
top level: pSB2K4
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974304/range1974304/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974304/range1974304/1
type: Range
persistent identity: range1974304
display id: range1974304
version: 1
direction: direction/reverse
top level: pSB2K4
start: 568
end: 586
orientation: inline

repE binding site
type: SequenceAnnotation
persistent identity: annotation1974307
display id: annotation1974307
version: 1
top level: pSB2K4
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974307/range1974307/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974307/range1974307/1
type: Range
persistent identity: range1974307
display id: range1974307
version: 1
direction: direction/reverse
top level: pSB2K4
start: 623
end: 641
orientation: inline

Predicted transcription start
type: SequenceAnnotation
persistent identity: annotation1974314
display id: annotation1974314
version: 1
top level: pSB2K4
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974314/range1974314/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974314/range1974314/1
type: Range
persistent identity: range1974314
display id: range1974314
version: 1
direction: direction/reverse
top level: pSB2K4
start: 1426
end: 1426
orientation: inline

repE
type: SequenceAnnotation
persistent identity: annotation1974310
display id: annotation1974310
version: 1
top level: pSB2K4
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974310/range1974310/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974310/range1974310/1
type: Range
persistent identity: range1974310
display id: range1974310
version: 1
direction: direction/reverse
top level: pSB2K4
start: 644
end: 1399
orientation: inline

Predicted transcription start
type: SequenceAnnotation
persistent identity: annotation1974343
display id: annotation1974343
version: 1
top level: pSB2K4
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974343/range1974343/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974343/range1974343/1
type: Range
persistent identity: range1974343
display id: range1974343
version: 1
direction: direction/forward
top level: pSB2K4
start: 3394
end: 3394
orientation: inline

repE binding site
type: SequenceAnnotation
persistent identity: annotation1974306
display id: annotation1974306
version: 1
top level: pSB2K4
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974306/range1974306/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974306/range1974306/1
type: Range
persistent identity: range1974306
display id: range1974306
version: 1
direction: direction/reverse
top level: pSB2K4
start: 597
end: 619
orientation: inline

C->T
type: SequenceAnnotation
persistent identity: annotation1974350
display id: annotation1974350
version: 1
top level: pSB2K4
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974350/range1974350/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974350/range1974350/1
type: Range
persistent identity: range1974350
display id: range1974350
version: 1
direction: direction/forward
top level: pSB2K4
start: 4119
end: 4119
orientation: inline

Possible -10
type: SequenceAnnotation
persistent identity: annotation1974317
display id: annotation1974317
version: 1
top level: pSB2K4
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974317/range1974317/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974317/range1974317/1
type: Range
persistent identity: range1974317
display id: range1974317
version: 1
direction: direction/forward
top level: pSB2K4
start: 1444
end: 1452
orientation: inline

VF2 (not an exact match, but works)
type: SequenceAnnotation
persistent identity: annotation1974351
display id: annotation1974351
version: 1
top level: pSB2K4
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974351/range1974351/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974351/range1974351/1
type: Range
persistent identity: range1974351
display id: range1974351
version: 1
direction: direction/forward
top level: pSB2K4
start: 4313
end: 4332
orientation: inline

nnpp promoter 2 (0.96)'
type: SequenceAnnotation
persistent identity: annotation1974341
display id: annotation1974341
version: 1
top level: pSB2K4
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974341/range1974341/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974341/range1974341/1
type: Range
persistent identity: range1974341
display id: range1974341
version: 1
direction: direction/forward
top level: pSB2K4
start: 3353
end: 3398
orientation: inline

Low GC region
type: SequenceAnnotation
persistent identity: annotation1974330
display id: annotation1974330
version: 1
top level: pSB2K4
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974330/range1974330/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974330/range1974330/1
type: Range
persistent identity: range1974330
display id: range1974330
version: 1
direction: direction/forward
top level: pSB2K4
start: 1823
end: 1844
orientation: inline

Maybe A or G
type: SequenceAnnotation
persistent identity: annotation1974332
display id: annotation1974332
version: 1
top level: pSB2K4
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974332/range1974332/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974332/range1974332/1
type: Range
persistent identity: range1974332
display id: range1974332
version: 1
direction: direction/forward
top level: pSB2K4
start: 2565
end: 2565
orientation: inline

Possible -10
type: SequenceAnnotation
persistent identity: annotation1974316
display id: annotation1974316
version: 1
top level: pSB2K4
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974316/range1974316/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974316/range1974316/1
type: Range
persistent identity: range1974316
display id: range1974316
version: 1
direction: direction/reverse
top level: pSB2K4
start: 1433
end: 1441
orientation: inline

repL
type: SequenceAnnotation
persistent identity: annotation1974335
display id: annotation1974335
version: 1
top level: pSB2K4
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974335/range1974335/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974335/range1974335/1
type: Range
persistent identity: range1974335
display id: range1974335
version: 1
direction: direction/forward
top level: pSB2K4
start: 2316
end: 3161
orientation: inline

incC
type: SequenceAnnotation
persistent identity: annotation1974302
display id: annotation1974302
version: 1
top level: pSB2K4
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974302/range1974302/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974302/range1974302/1
type: Range
persistent identity: range1974302
display id: range1974302
version: 1
direction: direction/reverse
top level: pSB2K4
start: 394
end: 641
orientation: inline

BioBrick suffix
type: SequenceAnnotation
persistent identity: annotation1974291
display id: annotation1974291
version: 1
top level: pSB2K4
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974291/range1974291/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974291/range1974291/1
type: Range
persistent identity: range1974291
display id: range1974291
version: 1
direction: direction/forward
top level: pSB2K4
start: 1
end: 21
orientation: inline

T->C
type: SequenceAnnotation
persistent identity: annotation1974305
display id: annotation1974305
version: 1
top level: pSB2K4
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974305/range1974305/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974305/range1974305/1
type: Range
persistent identity: range1974305
display id: range1974305
version: 1
direction: direction/forward
top level: pSB2K4
start: 578
end: 578
orientation: inline

G->A
type: SequenceAnnotation
persistent identity: annotation1974337
display id: annotation1974337
version: 1
top level: pSB2K4
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974337/range1974337/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974337/range1974337/1
type: Range
persistent identity: range1974337
display id: range1974337
version: 1
direction: direction/forward
top level: pSB2K4
start: 3017
end: 3017
orientation: inline

T->C
type: SequenceAnnotation
persistent identity: annotation1974336
display id: annotation1974336
version: 1
top level: pSB2K4
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974336/range1974336/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974336/range1974336/1
type: Range
persistent identity: range1974336
display id: range1974336
version: 1
direction: direction/forward
top level: pSB2K4
start: 2774
end: 2774
orientation: inline

repE binding site
type: SequenceAnnotation
persistent identity: annotation1974326
display id: annotation1974326
version: 1
top level: pSB2K4
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974326/range1974326/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974326/range1974326/1
type: Range
persistent identity: range1974326
display id: range1974326
version: 1
direction: direction/forward
top level: pSB2K4
start: 1552
end: 1570
orientation: inline

C->T
type: SequenceAnnotation
persistent identity: annotation1974313
display id: annotation1974313
version: 1
top level: pSB2K4
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974313/range1974313/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974313/range1974313/1
type: Range
persistent identity: range1974313
display id: range1974313
version: 1
direction: direction/forward
top level: pSB2K4
start: 1142
end: 1142
orientation: inline

repE binding site
type: SequenceAnnotation
persistent identity: annotation1974301
display id: annotation1974301
version: 1
top level: pSB2K4
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974301/range1974301/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974301/range1974301/1
type: Range
persistent identity: range1974301
display id: range1974301
version: 1
direction: direction/reverse
top level: pSB2K4
start: 477
end: 495
orientation: inline

G->C
type: SequenceAnnotation
persistent identity: annotation1974347
display id: annotation1974347
version: 1
top level: pSB2K4
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974347/range1974347/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974347/range1974347/1
type: Range
persistent identity: range1974347
display id: range1974347
version: 1
direction: direction/forward
top level: pSB2K4
start: 3849
end: 3849
orientation: inline

Predicted transcription start
type: SequenceAnnotation
persistent identity: annotation1974322
display id: annotation1974322
version: 1
top level: pSB2K4
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974322/range1974322/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974322/range1974322/1
type: Range
persistent identity: range1974322
display id: range1974322
version: 1
direction: direction/forward
top level: pSB2K4
start: 1460
end: 1460
orientation: inline

nnpp promoter 1 (.92)'
type: SequenceAnnotation
persistent identity: annotation1974344
display id: annotation1974344
version: 1
top level: pSB2K4
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974344/range1974344/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974344/range1974344/1
type: Range
persistent identity: range1974344
display id: range1974344
version: 1
direction: direction/forward
top level: pSB2K4
start: 3375
end: 3420
orientation: inline

Predicted -35
type: SequenceAnnotation
persistent identity: annotation1974340
display id: annotation1974340
version: 1
top level: pSB2K4
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974340/range1974340/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974340/range1974340/1
type: Range
persistent identity: range1974340
display id: range1974340
version: 1
direction: direction/forward
top level: pSB2K4
start: 3359
end: 3364
orientation: inline

E. coli his operon terminator
type: SequenceAnnotation
persistent identity: annotation1974292
display id: annotation1974292
version: 1
top level: pSB2K4
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974292/range1974292/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974292/range1974292/1
type: Range
persistent identity: range1974292
display id: range1974292
version: 1
direction: direction/forward
top level: pSB2K4
start: 51
end: 120
orientation: inline

nnpp promoter 3 (0.95)'
type: SequenceAnnotation
persistent identity: annotation1974339
display id: annotation1974339
version: 1
top level: pSB2K4
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974339/range1974339/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974339/range1974339/1
type: Range
persistent identity: range1974339
display id: range1974339
version: 1
direction: direction/forward
top level: pSB2K4
start: 3269
end: 3314
orientation: inline

T->A mutation
type: SequenceAnnotation
persistent identity: annotation1974303
display id: annotation1974303
version: 1
top level: pSB2K4
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974303/range1974303/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974303/range1974303/1
type: Range
persistent identity: range1974303
display id: range1974303
version: 1
direction: direction/forward
top level: pSB2K4
start: 551
end: 551
orientation: inline

Stem loop
type: SequenceAnnotation
persistent identity: annotation1974293
display id: annotation1974293
version: 1
top level: pSB2K4
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974293/range1974293/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974293/range1974293/1
type: Range
persistent identity: range1974293
display id: range1974293
version: 1
direction: direction/forward
top level: pSB2K4
start: 59
end: 91
orientation: inline

T->C
type: SequenceAnnotation
persistent identity: annotation1974346
display id: annotation1974346
version: 1
top level: pSB2K4
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974346/range1974346/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974346/range1974346/1
type: Range
persistent identity: range1974346
display id: range1974346
version: 1
direction: direction/forward
top level: pSB2K4
start: 3702
end: 3702
orientation: inline

A->G mutation
type: SequenceAnnotation
persistent identity: annotation1974300
display id: annotation1974300
version: 1
top level: pSB2K4
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974300/range1974300/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974300/range1974300/1
type: Range
persistent identity: range1974300
display id: range1974300
version: 1
direction: direction/forward
top level: pSB2K4
start: 462
end: 462
orientation: inline

Low GC region
type: SequenceAnnotation
persistent identity: annotation1974329
display id: annotation1974329
version: 1
top level: pSB2K4
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974329/range1974329/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974329/range1974329/1
type: Range
persistent identity: range1974329
display id: range1974329
version: 1
direction: direction/forward
top level: pSB2K4
start: 1636
end: 1682
orientation: inline

T->G
type: SequenceAnnotation
persistent identity: annotation1974333
display id: annotation1974333
version: 1
top level: pSB2K4
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974333/range1974333/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974333/range1974333/1
type: Range
persistent identity: range1974333
display id: range1974333
version: 1
direction: direction/forward
top level: pSB2K4
start: 2567
end: 2567
orientation: inline

A->C
type: SequenceAnnotation
persistent identity: annotation1974308
display id: annotation1974308
version: 1
top level: pSB2K4
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974308/range1974308/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974308/range1974308/1
type: Range
persistent identity: range1974308
display id: range1974308
version: 1
direction: direction/forward
top level: pSB2K4
start: 692
end: 692
orientation: inline

Predicted -10
type: SequenceAnnotation
persistent identity: annotation1974342
display id: annotation1974342
version: 1
top level: pSB2K4
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974342/range1974342/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974342/range1974342/1
type: Range
persistent identity: range1974342
display id: range1974342
version: 1
direction: direction/forward
top level: pSB2K4
start: 3379
end: 3387
orientation: inline

G->C
type: SequenceAnnotation
persistent identity: annotation1974352
display id: annotation1974352
version: 1
top level: pSB2K4
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974352/range1974352/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974352/range1974352/1
type: Range
persistent identity: range1974352
display id: range1974352
version: 1
direction: direction/forward
top level: pSB2K4
start: 4321
end: 4321
orientation: inline

C->A
type: SequenceAnnotation
persistent identity: annotation1974295
display id: annotation1974295
version: 1
top level: pSB2K4
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974295/range1974295/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974295/range1974295/1
type: Range
persistent identity: range1974295
display id: range1974295
version: 1
direction: direction/forward
top level: pSB2K4
start: 146
end: 146
orientation: inline

G->T
type: SequenceAnnotation
persistent identity: annotation1974319
display id: annotation1974319
version: 1
top level: pSB2K4
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974319/range1974319/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974319/range1974319/1
type: Range
persistent identity: range1974319
display id: range1974319
version: 1
direction: direction/forward
top level: pSB2K4
start: 1454
end: 1454
orientation: inline

T->A
type: SequenceAnnotation
persistent identity: annotation1974345
display id: annotation1974345
version: 1
top level: pSB2K4
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974345/range1974345/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974345/range1974345/1
type: Range
persistent identity: range1974345
display id: range1974345
version: 1
direction: direction/forward
top level: pSB2K4
start: 3507
end: 3507
orientation: inline

G->C
type: SequenceAnnotation
persistent identity: annotation1974309
display id: annotation1974309
version: 1
top level: pSB2K4
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974309/range1974309/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974309/range1974309/1
type: Range
persistent identity: range1974309
display id: range1974309
version: 1
direction: direction/forward
top level: pSB2K4
start: 857
end: 857
orientation: inline

lacOP
type: SequenceAnnotation
persistent identity: annotation1974331
display id: annotation1974331
version: 1
top level: pSB2K4
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974331/range1974331/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974331/range1974331/1
type: Range
persistent identity: range1974331
display id: range1974331
version: 1
direction: direction/forward
top level: pSB2K4
start: 2064
end: 2238
orientation: inline

repE binding site
type: SequenceAnnotation
persistent identity: annotation1974327
display id: annotation1974327
version: 1
top level: pSB2K4
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974327/range1974327/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974327/range1974327/1
type: Range
persistent identity: range1974327
display id: range1974327
version: 1
direction: direction/forward
top level: pSB2K4
start: 1581
end: 1591
orientation: inline

repE binding site
type: SequenceAnnotation
persistent identity: annotation1974324
display id: annotation1974324
version: 1
top level: pSB2K4
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974324/range1974324/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974324/range1974324/1
type: Range
persistent identity: range1974324
display id: range1974324
version: 1
direction: direction/forward
top level: pSB2K4
start: 1497
end: 1515
orientation: inline

T->C
type: SequenceAnnotation
persistent identity: annotation1974294
display id: annotation1974294
version: 1
top level: pSB2K4
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974294/range1974294/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974294/range1974294/1
type: Range
persistent identity: range1974294
display id: range1974294
version: 1
direction: direction/forward
top level: pSB2K4
start: 93
end: 93
orientation: inline

T->A
type: SequenceAnnotation
persistent identity: annotation1974298
display id: annotation1974298
version: 1
top level: pSB2K4
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974298/range1974298/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974298/range1974298/1
type: Range
persistent identity: range1974298
display id: range1974298
version: 1
direction: direction/forward
top level: pSB2K4
start: 292
end: 292
orientation: inline

G->C
type: SequenceAnnotation
persistent identity: annotation1974311
display id: annotation1974311
version: 1
top level: pSB2K4
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974311/range1974311/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974311/range1974311/1
type: Range
persistent identity: range1974311
display id: range1974311
version: 1
direction: direction/forward
top level: pSB2K4
start: 1091
end: 1091
orientation: inline

repE binding site
type: SequenceAnnotation
persistent identity: annotation1974299
display id: annotation1974299
version: 1
top level: pSB2K4
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974299/range1974299/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974299/range1974299/1
type: Range
persistent identity: range1974299
display id: range1974299
version: 1
direction: direction/reverse
top level: pSB2K4
start: 394
end: 412
orientation: inline

Possible -35
type: SequenceAnnotation
persistent identity: annotation1974321
display id: annotation1974321
version: 1
top level: pSB2K4
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974321/range1974321/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974321/range1974321/1
type: Range
persistent identity: range1974321
display id: range1974321
version: 1
direction: direction/reverse
top level: pSB2K4
start: 1455
end: 1460
orientation: inline

T->C
type: SequenceAnnotation
persistent identity: annotation1974312
display id: annotation1974312
version: 1
top level: pSB2K4
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974312/range1974312/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974312/range1974312/1
type: Range
persistent identity: range1974312
display id: range1974312
version: 1
direction: direction/forward
top level: pSB2K4
start: 1100
end: 1100
orientation: inline

kanR
type: SequenceAnnotation
persistent identity: annotation1974348
display id: annotation1974348
version: 1
top level: pSB2K4
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/pSB2K4/annotation1974348/range1974348/1

https://synbiohub.org/public/igem/pSB2K4/annotation1974348/range1974348/1
type: Range
persistent identity: range1974348
display id: range1974348
version: 1
direction: direction/forward
top level: pSB2K4
start: 3460
end: 4275
orientation: inline

pSB8K15FRT
type: ComponentDefinition
persistent identity: BBa K864022
display id: BBa_K864022
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Low copy BioBrick temperature sensitive standard vector
creator: Erik Lundin
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:37Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1124_
m user id: 0
owner id: 9827
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: awd
mutable notes: The resistance cassette is terminated by a Lux bidirectional terminator. Adjacent to this terminator there is a designed primer binding site to where the forward primer for lambda red can be designed to bind to no matter what the insert in the cloning site is or what the resistance is. The resistance cassette is flanked by SalI and SacI restriction sites for easy switching of resistance in the backbone. The pSC101ts origin of replication is flaked by NheI and MluI restriction sites for easy switching of origin of replication.
mutable provenance: Based on BBa_K864017
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence: https://synbiohub.org/public/igem/BBa_K864022_sequence/1

BBa_K864002
type: ComponentDefinition
persistent identity: BBa K864002
display id: BBa_K864002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Low copy BioBrick standard vector
creator: Erik Lundin
created: 2012-09-22T11:00:00Z
modified: 2015-05-08T01:13:37Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1124_
m user id: 0
owner id: 9827
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: pSB4K15 is a BioBrick standard vector with low copy pSC101 replication origin (~5 copies per cell) and kanamycin antibiotic resistance marker, especially usable for Lambda Red recombineering in E coli. The backbone sequence is based on pSB3T5, but the E coli His operon terminator BBa_B0053 has been replaced with the late terminator of the Salmonella phage P22.
mutable notes: The resistance cassette is terminated by a Lux bidirectional terminator. Adjacent to this terminator there is a designed primer binding site to where the forward primer for lambda red can be designed to bind to no matter what the insert in the cloning site is or what the resistance is. The resistance cassette is flanked by SalI and SacI restriction sites for easy switching of resistance in the backbone. The pSC101 origin of replication is flaked by NheI and MluI restriction sites for easy switching of origin of replication.
mutable provenance: Based on pSB3T5
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: pSC101 ori BBa_K864050
sequence annotation: Lambda Red forward primer
sequence annotation: VF2
sequence annotation: Lux terminator
sequence annotation: KanR BBa_P1003
sequence annotation: Lambda Red reverse primer
sequence annotation: P22 late terminator
sequence annotation: TonB terminator
sequence annotation: VR
sequence annotation: T3 terminator
sequence annotation: rrnC terminator
sequence: https://synbiohub.org/public/igem/BBa_K864002_sequence/1

pSC101 ori BBa_K864050
type: SequenceAnnotation
persistent identity: annotation2198835
display id: annotation2198835
version: 1
top level: BBa_K864002
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864002/annotation2198835/range2198835/1

https://synbiohub.org/public/igem/BBa_K864002/annotation2198835/range2198835/1
type: Range
persistent identity: range2198835
display id: range2198835
version: 1
direction: direction/forward
top level: BBa_K864002
start: 190
end: 2405
orientation: inline

Lambda Red forward primer
type: SequenceAnnotation
persistent identity: annotation2198836
display id: annotation2198836
version: 1
top level: BBa_K864002
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K864002/annotation2198836/range2198836/1

https://synbiohub.org/public/igem/BBa_K864002/annotation2198836/range2198836/1
type: Range
persistent identity: range2198836
display id: range2198836
version: 1
direction: direction/forward
top level: BBa_K864002
start: 2433
end: 2450
orientation: inline

VF2
type: SequenceAnnotation
persistent identity: annotation2198840
display id: annotation2198840
version: 1
top level: BBa_K864002
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K864002/annotation2198840/range2198840/1

https://synbiohub.org/public/igem/BBa_K864002/annotation2198840/range2198840/1
type: Range
persistent identity: range2198840
display id: range2198840
version: 1
direction: direction/forward
top level: BBa_K864002
start: 3519
end: 3538
orientation: inline

Lux terminator
type: SequenceAnnotation
persistent identity: annotation2198837
display id: annotation2198837
version: 1
top level: BBa_K864002
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K864002/annotation2198837/range2198837/1

https://synbiohub.org/public/igem/BBa_K864002/annotation2198837/range2198837/1
type: Range
persistent identity: range2198837
display id: range2198837
version: 1
direction: direction/reverse
top level: BBa_K864002
start: 2454
end: 2499
orientation: inline

KanR BBa_P1003
type: SequenceAnnotation
persistent identity: annotation2198838
display id: annotation2198838
version: 1
top level: BBa_K864002
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864002/annotation2198838/range2198838/1

https://synbiohub.org/public/igem/BBa_K864002/annotation2198838/range2198838/1
type: Range
persistent identity: range2198838
display id: range2198838
version: 1
direction: direction/forward
top level: BBa_K864002
start: 2506
end: 3224
orientation: inline

Lambda Red reverse primer
type: SequenceAnnotation
persistent identity: annotation2198831
display id: annotation2198831
version: 1
top level: BBa_K864002
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K864002/annotation2198831/range2198831/1

https://synbiohub.org/public/igem/BBa_K864002/annotation2198831/range2198831/1
type: Range
persistent identity: range2198831
display id: range2198831
version: 1
direction: direction/reverse
top level: BBa_K864002
start: 18
end: 38
orientation: inline

P22 late terminator
type: SequenceAnnotation
persistent identity: annotation2198839
display id: annotation2198839
version: 1
top level: BBa_K864002
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K864002/annotation2198839/range2198839/1

https://synbiohub.org/public/igem/BBa_K864002/annotation2198839/range2198839/1
type: Range
persistent identity: range2198839
display id: range2198839
version: 1
direction: direction/forward
top level: BBa_K864002
start: 3478
end: 3518
orientation: inline

TonB terminator
type: SequenceAnnotation
persistent identity: annotation2198832
display id: annotation2198832
version: 1
top level: BBa_K864002
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K864002/annotation2198832/range2198832/1

https://synbiohub.org/public/igem/BBa_K864002/annotation2198832/range2198832/1
type: Range
persistent identity: range2198832
display id: range2198832
version: 1
direction: direction/forward
top level: BBa_K864002
start: 47
end: 115
orientation: inline

VR
type: SequenceAnnotation
persistent identity: annotation2198833
display id: annotation2198833
version: 1
top level: BBa_K864002
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K864002/annotation2198833/range2198833/1

https://synbiohub.org/public/igem/BBa_K864002/annotation2198833/range2198833/1
type: Range
persistent identity: range2198833
display id: range2198833
version: 1
direction: direction/forward
top level: BBa_K864002
start: 116
end: 135
orientation: inline

T3 terminator
type: SequenceAnnotation
persistent identity: annotation2198841
display id: annotation2198841
version: 1
top level: BBa_K864002
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K864002/annotation2198841/range2198841/1

https://synbiohub.org/public/igem/BBa_K864002/annotation2198841/range2198841/1
type: Range
persistent identity: range2198841
display id: range2198841
version: 1
direction: direction/forward
top level: BBa_K864002
start: 3539
end: 3616
orientation: inline

rrnC terminator
type: SequenceAnnotation
persistent identity: annotation2198834
display id: annotation2198834
version: 1
top level: BBa_K864002
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K864002/annotation2198834/range2198834/1

https://synbiohub.org/public/igem/BBa_K864002/annotation2198834/range2198834/1
type: Range
persistent identity: range2198834
display id: range2198834
version: 1
direction: direction/forward
top level: BBa_K864002
start: 136
end: 175
orientation: inline

pSBBs4S
type: ComponentDefinition
persistent identity: BBa K823022
display id: BBa_K823022
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pSBBs4S: Empty backbone for integration into Bacillus subtilis thrC locus
creator: Jara Radeck
created: 2012-08-15T11:00:00Z
modified: 2015-05-08T01:13:30Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1081_
m user id: 0
owner id: 6378
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part is an empty backbone vector for the usage in Bacillus subtilis. It integrates in the thrC locus and can be selected with Spectinomycin cassette. It has a ampicillin resistance for cloning in E.coli. It originates from the Bacillus vector pDG1731.
mutable notes: This plasmid can be used as empty backbone vectors like pSB1C3 but is able to integrate in B.subtilis. The clonging site also includes a rfp for selection.
mutable provenance: B.subtilis vector pDG1731
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: bla
sequence annotation: 'thrC
sequence annotation: thrC'
sequence annotation: ori approx.
sequence annotation: spc
sequence annotation: thrB
sequence annotation: hom
sequence: https://synbiohub.org/public/igem/BBa_K823022_sequence/1

bla
type: SequenceAnnotation
persistent identity: annotation2179611
display id: annotation2179611
version: 1
top level: pSBBs4S
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K823022/annotation2179611/range2179611/1

https://synbiohub.org/public/igem/BBa_K823022/annotation2179611/range2179611/1
type: Range
persistent identity: range2179611
display id: range2179611
version: 1
direction: direction/reverse
top level: pSBBs4S
start: 2716
end: 3576
orientation: inline

'thrC
type: SequenceAnnotation
persistent identity: annotation2179608
display id: annotation2179608
version: 1
top level: pSBBs4S
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K823022/annotation2179608/range2179608/1

https://synbiohub.org/public/igem/BBa_K823022/annotation2179608/range2179608/1
type: Range
persistent identity: range2179608
display id: range2179608
version: 1
direction: direction/forward
top level: pSBBs4S
start: 1028
end: 1456
orientation: inline

thrC'
type: SequenceAnnotation
persistent identity: annotation2179613
display id: annotation2179613
version: 1
top level: pSBBs4S
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K823022/annotation2179613/range2179613/1

https://synbiohub.org/public/igem/BBa_K823022/annotation2179613/range2179613/1
type: Range
persistent identity: range2179613
display id: range2179613
version: 1
direction: direction/forward
top level: pSBBs4S
start: 4033
end: 4542
orientation: inline

ori approx.
type: SequenceAnnotation
persistent identity: annotation2179610
display id: annotation2179610
version: 1
top level: pSBBs4S
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K823022/annotation2179610/range2179610/1

https://synbiohub.org/public/igem/BBa_K823022/annotation2179610/range2179610/1
type: Range
persistent identity: range2179610
display id: range2179610
version: 1
direction: direction/forward
top level: pSBBs4S
start: 1942
end: 2556
orientation: inline

spc
type: SequenceAnnotation
persistent identity: annotation2179607
display id: annotation2179607
version: 1
top level: pSBBs4S
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K823022/annotation2179607/range2179607/1

https://synbiohub.org/public/igem/BBa_K823022/annotation2179607/range2179607/1
type: Range
persistent identity: range2179607
display id: range2179607
version: 1
direction: direction/forward
top level: pSBBs4S
start: 211
end: 885
orientation: inline

thrB
type: SequenceAnnotation
persistent identity: annotation2179609
display id: annotation2179609
version: 1
top level: pSBBs4S
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K823022/annotation2179609/range2179609/1

https://synbiohub.org/public/igem/BBa_K823022/annotation2179609/range2179609/1
type: Range
persistent identity: range2179609
display id: range2179609
version: 1
direction: direction/forward
top level: pSBBs4S
start: 1453
end: 1863
orientation: inline

hom
type: SequenceAnnotation
persistent identity: annotation2179612
display id: annotation2179612
version: 1
top level: pSBBs4S
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K823022/annotation2179612/range2179612/1

https://synbiohub.org/public/igem/BBa_K823022/annotation2179612/range2179612/1
type: Range
persistent identity: range2179612
display id: range2179612
version: 1
direction: direction/forward
top level: pSBBs4S
start: 3731
end: 4033
orientation: inline

BBa_K864012
type: ComponentDefinition
persistent identity: BBa K864012
display id: BBa_K864012
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Low copy BioBrick standard vector with LacI
creator: Erik Lundin
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:37Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1124_
m user id: 0
owner id: 9827
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: pSB4A15Iq(FRT) is a BioBrick standard vector with low copy pSC101 replication origin (~5 copies per cell) and ampicillin antibiotic resistance marker, especially usable for Lambda Red recombineering in E coli. The backbone sequence is based on pSB3T5, but the E coli His operon terminator BBa_B0053 has been replaced with the late terminator of the Salmonella phage P22. This vector carries a LacIq cassette on the backbone for tight repression of any lac promoter cloned into the cloning site.
mutable notes: The resistance cassette is terminated by a Lux bidirectional terminator. Adjacent to this terminator there is a designed primer binding site to where the forward primer for lambda red can be designed to bind to no matter what the insert in the cloning site is or what the resistance is. The resistance cassette is flanked by SalI and SacI restriction sites for easy switching of resistance in the backbone.
mutable provenance: Based on BBa_K864005
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence: https://synbiohub.org/public/igem/BBa_K864012_sequence/1

pSB3T5
type: ComponentDefinition
persistent identity: pSB3T5
display id: pSB3T5
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Low to medium copy BioBrick standard vector
creator: Reshma Shetty
created: 2007-04-10T11:00:00Z
modified: 2015-05-08T01:14:55Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _41_
m user id: 0
owner id: 126
owning group id: 70
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: pSB3T5 is a low to medium copy plasmid based with tetracycline resistance. It has a p15A-derived replication origin. pSB3T5 is derived from the BioBricks vector scaffold BBa_I51001.
mutable notes: None.
mutable provenance: BBa_I51001 BioBricks vector scaffold, p15A based origin and a version of the tetracycline resistance cassette that has been cleaned of restriction sites.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
component: BBa_I50032
component: BBa_B0054
component: BBa_B0053
component: BBa_B0043
component: BBa_B0055
component: BBa_B0044
component: BBa_G00000
component: BBa_G00102
component: BBa_B0042
component: BBa_G0002
component: BBa_G00001
component: BBa_P1005
component: BBa_G0004
component: BBa_B0062
component: BBa_G00100
component: BBa_G0003
sequence annotation: BBa_B0042
sequence annotation: BBa_B0044
sequence annotation: BBa_B0055
sequence annotation: BBa_P1005
sequence annotation: BBa_G00100
sequence annotation: BBa_G0002
sequence annotation: BBa_I50032
sequence annotation: BBa_B0062
sequence annotation: BBa_G00102
sequence annotation: BBa_B0043
sequence annotation: BBa_B0053
sequence annotation: BBa_G0003
sequence annotation: BBa_B0054
sequence annotation: BBa_G00001
sequence annotation: BBa_G00000
sequence annotation: BBa_G0004
sequence: https://synbiohub.org/public/igem/pSB3T5_sequence/1

BBa_I50032
type: Component
persistent identity: component1928380
display id: component1928380
version: 1
top level: pSB3T5
access: public
definition: https://synbiohub.org/public/igem/BBa_I50032/1

BBa_B0054
type: Component
persistent identity: component1928373
display id: component1928373
version: 1
top level: pSB3T5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0054/1

BBa_B0053
type: Component
persistent identity: component1928387
display id: component1928387
version: 1
top level: pSB3T5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0053/1

BBa_B0043
type: Component
persistent identity: component1928403
display id: component1928403
version: 1
top level: pSB3T5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0043/1

BBa_B0055
type: Component
persistent identity: component1928391
display id: component1928391
version: 1
top level: pSB3T5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0055/1

BBa_B0044
type: Component
persistent identity: component1928363
display id: component1928363
version: 1
top level: pSB3T5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0044/1

BBa_G00000
type: Component
persistent identity: component1928405
display id: component1928405
version: 1
top level: pSB3T5
access: public
definition: https://synbiohub.org/public/igem/BBa_G00000/1

BBa_G00102
type: Component
persistent identity: component1928374
display id: component1928374
version: 1
top level: pSB3T5
access: public
definition: https://synbiohub.org/public/igem/BBa_G00102/1

BBa_B0042
type: Component
persistent identity: component1928398
display id: component1928398
version: 1
top level: pSB3T5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0042/1

BBa_G0002
type: Component
persistent identity: component1928381
display id: component1928381
version: 1
top level: pSB3T5
access: public
definition: https://synbiohub.org/public/igem/BBa_G0002/1

BBa_G00001
type: Component
persistent identity: component1928358
display id: component1928358
version: 1
top level: pSB3T5
access: public
definition: https://synbiohub.org/public/igem/BBa_G00001/1

BBa_P1005
type: Component
persistent identity: component1928384
display id: component1928384
version: 1
top level: pSB3T5
access: public
definition: https://synbiohub.org/public/igem/BBa_P1005/1

BBa_G0004
type: Component
persistent identity: component1928385
display id: component1928385
version: 1
top level: pSB3T5
access: public
definition: https://synbiohub.org/public/igem/BBa_G0004/1

BBa_B0062
type: Component
persistent identity: component1928376
display id: component1928376
version: 1
top level: pSB3T5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0062/1

BBa_B0042
type: Component
persistent identity: component1928370
display id: component1928370
version: 1
top level: pSB3T5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0042/1

BBa_G00100
type: Component
persistent identity: component1928388
display id: component1928388
version: 1
top level: pSB3T5
access: public
definition: https://synbiohub.org/public/igem/BBa_G00100/1

BBa_G0003
type: Component
persistent identity: component1928377
display id: component1928377
version: 1
top level: pSB3T5
access: public
definition: https://synbiohub.org/public/igem/BBa_G0003/1

BBa_B0042
type: SequenceAnnotation
persistent identity: annotation1928398
display id: annotation1928398
version: 1
top level: pSB3T5
location: https://synbiohub.org/public/igem/pSB3T5/annotation1928398/range1928398/1
component: BBa_B0042

https://synbiohub.org/public/igem/pSB3T5/annotation1928398/range1928398/1
type: Range
persistent identity: range1928398
display id: range1928398
version: 1
direction: direction/reverse
top level: pSB3T5
start: 3206
end: 3217
orientation: inline

BBa_B0044
type: SequenceAnnotation
persistent identity: annotation1928363
display id: annotation1928363
version: 1
top level: pSB3T5
location: https://synbiohub.org/public/igem/pSB3T5/annotation1928363/range1928363/1
component: BBa_B0044

https://synbiohub.org/public/igem/pSB3T5/annotation1928363/range1928363/1
type: Range
persistent identity: range1928363
display id: range1928363
version: 1
direction: direction/reverse
top level: pSB3T5
start: 22
end: 34
orientation: inline

BBa_B0055
type: SequenceAnnotation
persistent identity: annotation1928391
display id: annotation1928391
version: 1
top level: pSB3T5
location: https://synbiohub.org/public/igem/pSB3T5/annotation1928391/range1928391/1
component: BBa_B0055

https://synbiohub.org/public/igem/pSB3T5/annotation1928391/range1928391/1
type: Range
persistent identity: range1928391
display id: range1928391
version: 1
direction: direction/reverse
top level: pSB3T5
start: 3128
end: 3205
orientation: inline

BBa_P1005
type: SequenceAnnotation
persistent identity: annotation1928384
display id: annotation1928384
version: 1
top level: pSB3T5
location: https://synbiohub.org/public/igem/pSB3T5/annotation1928384/range1928384/1
component: BBa_P1005

https://synbiohub.org/public/igem/pSB3T5/annotation1928384/range1928384/1
type: Range
persistent identity: range1928384
display id: range1928384
version: 1
direction: direction/reverse
top level: pSB3T5
start: 1746
end: 3028
orientation: inline

BBa_G00100
type: SequenceAnnotation
persistent identity: annotation1928388
display id: annotation1928388
version: 1
top level: pSB3T5
location: https://synbiohub.org/public/igem/pSB3T5/annotation1928388/range1928388/1
component: BBa_G00100

https://synbiohub.org/public/igem/pSB3T5/annotation1928388/range1928388/1
type: Range
persistent identity: range1928388
display id: range1928388
version: 1
direction: direction/reverse
top level: pSB3T5
start: 3108
end: 3127
orientation: inline

BBa_G0002
type: SequenceAnnotation
persistent identity: annotation1928381
display id: annotation1928381
version: 1
top level: pSB3T5
location: https://synbiohub.org/public/igem/pSB3T5/annotation1928381/range1928381/1
component: BBa_G0002

https://synbiohub.org/public/igem/pSB3T5/annotation1928381/range1928381/1
type: Range
persistent identity: range1928381
display id: range1928381
version: 1
direction: direction/reverse
top level: pSB3T5
start: 1738
end: 1745
orientation: inline

BBa_I50032
type: SequenceAnnotation
persistent identity: annotation1928380
display id: annotation1928380
version: 1
top level: pSB3T5
location: https://synbiohub.org/public/igem/pSB3T5/annotation1928380/range1928380/1
component: BBa_I50032

https://synbiohub.org/public/igem/pSB3T5/annotation1928380/range1928380/1
type: Range
persistent identity: range1928380
display id: range1928380
version: 1
direction: direction/reverse
top level: pSB3T5
start: 184
end: 1737
orientation: inline

BBa_B0062
type: SequenceAnnotation
persistent identity: annotation1928376
display id: annotation1928376
version: 1
top level: pSB3T5
location: https://synbiohub.org/public/igem/pSB3T5/annotation1928376/range1928376/1
component: BBa_B0062

https://synbiohub.org/public/igem/pSB3T5/annotation1928376/range1928376/1
type: Range
persistent identity: range1928376
display id: range1928376
version: 1
direction: direction/reverse
top level: pSB3T5
start: 136
end: 176
orientation: inline

BBa_G00102
type: SequenceAnnotation
persistent identity: annotation1928374
display id: annotation1928374
version: 1
top level: pSB3T5
location: https://synbiohub.org/public/igem/pSB3T5/annotation1928374/range1928374/1
component: BBa_G00102

https://synbiohub.org/public/igem/pSB3T5/annotation1928374/range1928374/1
type: Range
persistent identity: range1928374
display id: range1928374
version: 1
direction: direction/reverse
top level: pSB3T5
start: 116
end: 135
orientation: inline

BBa_B0042
type: SequenceAnnotation
persistent identity: annotation1928370
display id: annotation1928370
version: 1
top level: pSB3T5
location: https://synbiohub.org/public/igem/pSB3T5/annotation1928370/range1928370/1
component: BBa_B0042

https://synbiohub.org/public/igem/pSB3T5/annotation1928370/range1928370/1
type: Range
persistent identity: range1928370
display id: range1928370
version: 1
direction: direction/reverse
top level: pSB3T5
start: 35
end: 46
orientation: inline

BBa_B0043
type: SequenceAnnotation
persistent identity: annotation1928403
display id: annotation1928403
version: 1
top level: pSB3T5
location: https://synbiohub.org/public/igem/pSB3T5/annotation1928403/range1928403/1
component: BBa_B0043

https://synbiohub.org/public/igem/pSB3T5/annotation1928403/range1928403/1
type: Range
persistent identity: range1928403
display id: range1928403
version: 1
direction: direction/reverse
top level: pSB3T5
start: 3218
end: 3230
orientation: inline

BBa_B0053
type: SequenceAnnotation
persistent identity: annotation1928387
display id: annotation1928387
version: 1
top level: pSB3T5
location: https://synbiohub.org/public/igem/pSB3T5/annotation1928387/range1928387/1
component: BBa_B0053

https://synbiohub.org/public/igem/pSB3T5/annotation1928387/range1928387/1
type: Range
persistent identity: range1928387
display id: range1928387
version: 1
direction: direction/reverse
top level: pSB3T5
start: 3036
end: 3107
orientation: inline

BBa_G0003
type: SequenceAnnotation
persistent identity: annotation1928377
display id: annotation1928377
version: 1
top level: pSB3T5
location: https://synbiohub.org/public/igem/pSB3T5/annotation1928377/range1928377/1
component: BBa_G0003

https://synbiohub.org/public/igem/pSB3T5/annotation1928377/range1928377/1
type: Range
persistent identity: range1928377
display id: range1928377
version: 1
direction: direction/reverse
top level: pSB3T5
start: 177
end: 183
orientation: inline

BBa_B0054
type: SequenceAnnotation
persistent identity: annotation1928373
display id: annotation1928373
version: 1
top level: pSB3T5
location: https://synbiohub.org/public/igem/pSB3T5/annotation1928373/range1928373/1
component: BBa_B0054

https://synbiohub.org/public/igem/pSB3T5/annotation1928373/range1928373/1
type: Range
persistent identity: range1928373
display id: range1928373
version: 1
direction: direction/reverse
top level: pSB3T5
start: 47
end: 115
orientation: inline

BBa_G00001
type: SequenceAnnotation
persistent identity: annotation1928358
display id: annotation1928358
version: 1
top level: pSB3T5
location: https://synbiohub.org/public/igem/pSB3T5/annotation1928358/range1928358/1
component: BBa_G00001

https://synbiohub.org/public/igem/pSB3T5/annotation1928358/range1928358/1
type: Range
persistent identity: range1928358
display id: range1928358
version: 1
direction: direction/reverse
top level: pSB3T5
start: 1
end: 21
orientation: inline

BBa_G00000
type: SequenceAnnotation
persistent identity: annotation1928405
display id: annotation1928405
version: 1
top level: pSB3T5
location: https://synbiohub.org/public/igem/pSB3T5/annotation1928405/range1928405/1
component: BBa_G00000

https://synbiohub.org/public/igem/pSB3T5/annotation1928405/range1928405/1
type: Range
persistent identity: range1928405
display id: range1928405
version: 1
direction: direction/reverse
top level: pSB3T5
start: 3231
end: 3252
orientation: inline

BBa_G0004
type: SequenceAnnotation
persistent identity: annotation1928385
display id: annotation1928385
version: 1
top level: pSB3T5
location: https://synbiohub.org/public/igem/pSB3T5/annotation1928385/range1928385/1
component: BBa_G0004

https://synbiohub.org/public/igem/pSB3T5/annotation1928385/range1928385/1
type: Range
persistent identity: range1928385
display id: range1928385
version: 1
direction: direction/reverse
top level: pSB3T5
start: 3029
end: 3035
orientation: inline

pTG262-BB
type: ComponentDefinition
persistent identity: BBa I742123
display id: BBa_I742123
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Multi-host vector pTG262 converted to BioBrick vector
creator: Sarah Hollingshead
created: 2007-10-18T11:00:00Z
modified: 2015-08-31T04:08:02Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _123_
m user id: 0
owner id: 1964
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: biobricked version of pTG262 Plasmid has a multi host replication origin and is competant in range of gram positive and gram negative bacteria.
mutable notes: n/a
mutable provenance: We kindly thank Dr Mike Gasson & Dr. Claire Shearman, of the Institute of Food Research in Norwich for generously donating the pTG262 vector.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: BioBrick prefix
sequence annotation: cat
sequence annotation: BioBrick suffix
sequence annotation: neo
sequence annotation: repA
sequence: https://synbiohub.org/public/igem/BBa_I742123_sequence/1

BioBrick prefix
type: SequenceAnnotation
persistent identity: annotation1974398
display id: annotation1974398
version: 1
top level: pTG262-BB
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_I742123/annotation1974398/range1974398/1

https://synbiohub.org/public/igem/BBa_I742123/annotation1974398/range1974398/1
type: Range
persistent identity: range1974398
display id: range1974398
version: 1
direction: direction/forward
top level: pTG262-BB
start: 5543
end: 5564
orientation: inline

cat
type: SequenceAnnotation
persistent identity: annotation1974397
display id: annotation1974397
version: 1
top level: pTG262-BB
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_I742123/annotation1974397/range1974397/1

https://synbiohub.org/public/igem/BBa_I742123/annotation1974397/range1974397/1
type: Range
persistent identity: range1974397
display id: range1974397
version: 1
direction: direction/forward
top level: pTG262-BB
start: 4444
end: 5094
orientation: inline

BioBrick suffix
type: SequenceAnnotation
persistent identity: annotation1974394
display id: annotation1974394
version: 1
top level: pTG262-BB
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_I742123/annotation1974394/range1974394/1

https://synbiohub.org/public/igem/BBa_I742123/annotation1974394/range1974394/1
type: Range
persistent identity: range1974394
display id: range1974394
version: 1
direction: direction/forward
top level: pTG262-BB
start: 1
end: 21
orientation: inline

neo
type: SequenceAnnotation
persistent identity: annotation1974396
display id: annotation1974396
version: 1
top level: pTG262-BB
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_I742123/annotation1974396/range1974396/1

https://synbiohub.org/public/igem/BBa_I742123/annotation1974396/range1974396/1
type: Range
persistent identity: range1974396
display id: range1974396
version: 1
direction: direction/forward
top level: pTG262-BB
start: 3149
end: 3919
orientation: inline

repA
type: SequenceAnnotation
persistent identity: annotation1974395
display id: annotation1974395
version: 1
top level: pTG262-BB
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_I742123/annotation1974395/range1974395/1

https://synbiohub.org/public/igem/BBa_I742123/annotation1974395/range1974395/1
type: Range
persistent identity: range1974395
display id: range1974395
version: 1
direction: direction/forward
top level: pTG262-BB
start: 1724
end: 2431
orientation: inline

pSB3K5
type: ComponentDefinition
persistent identity: pSB3K5
display id: pSB3K5
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Low to medium copy BioBrick standard vector
creator: Reshma Shetty
created: 2007-05-02T11:00:00Z
modified: 2015-05-08T01:14:55Z
discontinued: false
dominant: false
experience: experience/None
group u list: _41_
m user id: 0
owner id: 126
owning group id: 70
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: pSB3K5 is a low to medium copy plasmid based with kanamycin resistance. It has a p15A-derived replication origin. pSB3K5 is derived from the BioBricks vector scaffold BBa_I51001.
mutable notes: None.
mutable provenance: BBa_I51001 BioBricks vector scaffold, p15A based origin and a version of the kanamycin resistance cassette that has been cleaned of restriction sites.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
component: BBa_B0044
component: BBa_B0042
component: BBa_B0053
component: BBa_I50032
component: BBa_G0003
component: BBa_B0054
component: BBa_B0055
component: BBa_B0062
component: BBa_B0043
component: BBa_G00100
component: BBa_G00000
component: BBa_P1003
component: BBa_G00001
component: BBa_G00102
component: BBa_G0004
component: BBa_G0002
sequence annotation: BBa_B0042
sequence annotation: BBa_G00000
sequence annotation: BBa_B0062
sequence annotation: BBa_B0044
sequence annotation: BBa_B0054
sequence annotation: BBa_G00100
sequence annotation: BBa_B0053
sequence annotation: BBa_G00001
sequence annotation: BBa_G0002
sequence annotation: BBa_B0055
sequence annotation: BBa_G0003
sequence annotation: BBa_P1003
sequence annotation: BBa_I50032
sequence annotation: BBa_B0043
sequence annotation: BBa_G0004
sequence annotation: BBa_G00102
sequence: https://synbiohub.org/public/igem/pSB3K5_sequence/1

BBa_B0044
type: Component
persistent identity: component1932083
display id: component1932083
version: 1
top level: pSB3K5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0044/1

BBa_B0042
type: Component
persistent identity: component1932090
display id: component1932090
version: 1
top level: pSB3K5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0042/1

BBa_B0053
type: Component
persistent identity: component1932106
display id: component1932106
version: 1
top level: pSB3K5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0053/1

BBa_I50032
type: Component
persistent identity: component1932100
display id: component1932100
version: 1
top level: pSB3K5
access: public
definition: https://synbiohub.org/public/igem/BBa_I50032/1

BBa_G0003
type: Component
persistent identity: component1932097
display id: component1932097
version: 1
top level: pSB3K5
access: public
definition: https://synbiohub.org/public/igem/BBa_G0003/1

BBa_B0054
type: Component
persistent identity: component1932093
display id: component1932093
version: 1
top level: pSB3K5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0054/1

BBa_B0055
type: Component
persistent identity: component1932110
display id: component1932110
version: 1
top level: pSB3K5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0055/1

BBa_B0042
type: Component
persistent identity: component1932117
display id: component1932117
version: 1
top level: pSB3K5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0042/1

BBa_B0062
type: Component
persistent identity: component1932096
display id: component1932096
version: 1
top level: pSB3K5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0062/1

BBa_B0043
type: Component
persistent identity: component1932122
display id: component1932122
version: 1
top level: pSB3K5
access: public
definition: https://synbiohub.org/public/igem/BBa_B0043/1

BBa_G00100
type: Component
persistent identity: component1932107
display id: component1932107
version: 1
top level: pSB3K5
access: public
definition: https://synbiohub.org/public/igem/BBa_G00100/1

BBa_G00000
type: Component
persistent identity: component1932124
display id: component1932124
version: 1
top level: pSB3K5
access: public
definition: https://synbiohub.org/public/igem/BBa_G00000/1

BBa_P1003
type: Component
persistent identity: component1932103
display id: component1932103
version: 1
top level: pSB3K5
access: public
definition: https://synbiohub.org/public/igem/BBa_P1003/1

BBa_G00001
type: Component
persistent identity: component1932078
display id: component1932078
version: 1
top level: pSB3K5
access: public
definition: https://synbiohub.org/public/igem/BBa_G00001/1

BBa_G00102
type: Component
persistent identity: component1932094
display id: component1932094
version: 1
top level: pSB3K5
access: public
definition: https://synbiohub.org/public/igem/BBa_G00102/1

BBa_G0004
type: Component
persistent identity: component1932104
display id: component1932104
version: 1
top level: pSB3K5
access: public
definition: https://synbiohub.org/public/igem/BBa_G0004/1

BBa_G0002
type: Component
persistent identity: component1932101
display id: component1932101
version: 1
top level: pSB3K5
access: public
definition: https://synbiohub.org/public/igem/BBa_G0002/1

BBa_B0042
type: SequenceAnnotation
persistent identity: annotation1932117
display id: annotation1932117
version: 1
top level: pSB3K5
location: https://synbiohub.org/public/igem/pSB3K5/annotation1932117/range1932117/1
component: BBa_B0042

https://synbiohub.org/public/igem/pSB3K5/annotation1932117/range1932117/1
type: Range
persistent identity: range1932117
display id: range1932117
version: 1
direction: direction/reverse
top level: pSB3K5
start: 2890
end: 2901
orientation: inline

BBa_G00000
type: SequenceAnnotation
persistent identity: annotation1932124
display id: annotation1932124
version: 1
top level: pSB3K5
location: https://synbiohub.org/public/igem/pSB3K5/annotation1932124/range1932124/1
component: BBa_G00000

https://synbiohub.org/public/igem/pSB3K5/annotation1932124/range1932124/1
type: Range
persistent identity: range1932124
display id: range1932124
version: 1
direction: direction/reverse
top level: pSB3K5
start: 2915
end: 2936
orientation: inline

BBa_B0062
type: SequenceAnnotation
persistent identity: annotation1932096
display id: annotation1932096
version: 1
top level: pSB3K5
location: https://synbiohub.org/public/igem/pSB3K5/annotation1932096/range1932096/1
component: BBa_B0062

https://synbiohub.org/public/igem/pSB3K5/annotation1932096/range1932096/1
type: Range
persistent identity: range1932096
display id: range1932096
version: 1
direction: direction/reverse
top level: pSB3K5
start: 136
end: 176
orientation: inline

BBa_B0044
type: SequenceAnnotation
persistent identity: annotation1932083
display id: annotation1932083
version: 1
top level: pSB3K5
location: https://synbiohub.org/public/igem/pSB3K5/annotation1932083/range1932083/1
component: BBa_B0044

https://synbiohub.org/public/igem/pSB3K5/annotation1932083/range1932083/1
type: Range
persistent identity: range1932083
display id: range1932083
version: 1
direction: direction/reverse
top level: pSB3K5
start: 22
end: 34
orientation: inline

BBa_B0054
type: SequenceAnnotation
persistent identity: annotation1932093
display id: annotation1932093
version: 1
top level: pSB3K5
location: https://synbiohub.org/public/igem/pSB3K5/annotation1932093/range1932093/1
component: BBa_B0054

https://synbiohub.org/public/igem/pSB3K5/annotation1932093/range1932093/1
type: Range
persistent identity: range1932093
display id: range1932093
version: 1
direction: direction/reverse
top level: pSB3K5
start: 47
end: 115
orientation: inline

BBa_G00100
type: SequenceAnnotation
persistent identity: annotation1932107
display id: annotation1932107
version: 1
top level: pSB3K5
location: https://synbiohub.org/public/igem/pSB3K5/annotation1932107/range1932107/1
component: BBa_G00100

https://synbiohub.org/public/igem/pSB3K5/annotation1932107/range1932107/1
type: Range
persistent identity: range1932107
display id: range1932107
version: 1
direction: direction/reverse
top level: pSB3K5
start: 2792
end: 2811
orientation: inline

BBa_B0042
type: SequenceAnnotation
persistent identity: annotation1932090
display id: annotation1932090
version: 1
top level: pSB3K5
location: https://synbiohub.org/public/igem/pSB3K5/annotation1932090/range1932090/1
component: BBa_B0042

https://synbiohub.org/public/igem/pSB3K5/annotation1932090/range1932090/1
type: Range
persistent identity: range1932090
display id: range1932090
version: 1
direction: direction/reverse
top level: pSB3K5
start: 35
end: 46
orientation: inline

BBa_B0053
type: SequenceAnnotation
persistent identity: annotation1932106
display id: annotation1932106
version: 1
top level: pSB3K5
location: https://synbiohub.org/public/igem/pSB3K5/annotation1932106/range1932106/1
component: BBa_B0053

https://synbiohub.org/public/igem/pSB3K5/annotation1932106/range1932106/1
type: Range
persistent identity: range1932106
display id: range1932106
version: 1
direction: direction/reverse
top level: pSB3K5
start: 2720
end: 2791
orientation: inline

BBa_G00001
type: SequenceAnnotation
persistent identity: annotation1932078
display id: annotation1932078
version: 1
top level: pSB3K5
location: https://synbiohub.org/public/igem/pSB3K5/annotation1932078/range1932078/1
component: BBa_G00001

https://synbiohub.org/public/igem/pSB3K5/annotation1932078/range1932078/1
type: Range
persistent identity: range1932078
display id: range1932078
version: 1
direction: direction/reverse
top level: pSB3K5
start: 1
end: 21
orientation: inline

BBa_G0002
type: SequenceAnnotation
persistent identity: annotation1932101
display id: annotation1932101
version: 1
top level: pSB3K5
location: https://synbiohub.org/public/igem/pSB3K5/annotation1932101/range1932101/1
component: BBa_G0002

https://synbiohub.org/public/igem/pSB3K5/annotation1932101/range1932101/1
type: Range
persistent identity: range1932101
display id: range1932101
version: 1
direction: direction/reverse
top level: pSB3K5
start: 1738
end: 1745
orientation: inline

BBa_B0055
type: SequenceAnnotation
persistent identity: annotation1932110
display id: annotation1932110
version: 1
top level: pSB3K5
location: https://synbiohub.org/public/igem/pSB3K5/annotation1932110/range1932110/1
component: BBa_B0055

https://synbiohub.org/public/igem/pSB3K5/annotation1932110/range1932110/1
type: Range
persistent identity: range1932110
display id: range1932110
version: 1
direction: direction/reverse
top level: pSB3K5
start: 2812
end: 2889
orientation: inline

BBa_G0003
type: SequenceAnnotation
persistent identity: annotation1932097
display id: annotation1932097
version: 1
top level: pSB3K5
location: https://synbiohub.org/public/igem/pSB3K5/annotation1932097/range1932097/1
component: BBa_G0003

https://synbiohub.org/public/igem/pSB3K5/annotation1932097/range1932097/1
type: Range
persistent identity: range1932097
display id: range1932097
version: 1
direction: direction/reverse
top level: pSB3K5
start: 177
end: 183
orientation: inline

BBa_P1003
type: SequenceAnnotation
persistent identity: annotation1932103
display id: annotation1932103
version: 1
top level: pSB3K5
location: https://synbiohub.org/public/igem/pSB3K5/annotation1932103/range1932103/1
component: BBa_P1003

https://synbiohub.org/public/igem/pSB3K5/annotation1932103/range1932103/1
type: Range
persistent identity: range1932103
display id: range1932103
version: 1
direction: direction/reverse
top level: pSB3K5
start: 1746
end: 2712
orientation: inline

BBa_I50032
type: SequenceAnnotation
persistent identity: annotation1932100
display id: annotation1932100
version: 1
top level: pSB3K5
location: https://synbiohub.org/public/igem/pSB3K5/annotation1932100/range1932100/1
component: BBa_I50032

https://synbiohub.org/public/igem/pSB3K5/annotation1932100/range1932100/1
type: Range
persistent identity: range1932100
display id: range1932100
version: 1
direction: direction/reverse
top level: pSB3K5
start: 184
end: 1737
orientation: inline

BBa_B0043
type: SequenceAnnotation
persistent identity: annotation1932122
display id: annotation1932122
version: 1
top level: pSB3K5
location: https://synbiohub.org/public/igem/pSB3K5/annotation1932122/range1932122/1
component: BBa_B0043

https://synbiohub.org/public/igem/pSB3K5/annotation1932122/range1932122/1
type: Range
persistent identity: range1932122
display id: range1932122
version: 1
direction: direction/reverse
top level: pSB3K5
start: 2902
end: 2914
orientation: inline

BBa_G0004
type: SequenceAnnotation
persistent identity: annotation1932104
display id: annotation1932104
version: 1
top level: pSB3K5
location: https://synbiohub.org/public/igem/pSB3K5/annotation1932104/range1932104/1
component: BBa_G0004

https://synbiohub.org/public/igem/pSB3K5/annotation1932104/range1932104/1
type: Range
persistent identity: range1932104
display id: range1932104
version: 1
direction: direction/reverse
top level: pSB3K5
start: 2713
end: 2719
orientation: inline

BBa_G00102
type: SequenceAnnotation
persistent identity: annotation1932094
display id: annotation1932094
version: 1
top level: pSB3K5
location: https://synbiohub.org/public/igem/pSB3K5/annotation1932094/range1932094/1
component: BBa_G00102

https://synbiohub.org/public/igem/pSB3K5/annotation1932094/range1932094/1
type: Range
persistent identity: range1932094
display id: range1932094
version: 1
direction: direction/reverse
top level: pSB3K5
start: 116
end: 135
orientation: inline

pSB2K3
type: ComponentDefinition
persistent identity: pSB2K3
display id: pSB2K3
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Inducible copy number BioBrick plasmid
creator: Sri Kosuri
created: 2004-05-03T11:00:00Z
modified: 2015-05-08T01:14:55Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _11_48_46_1_
m user id: 0
owner id: 52
owning group id: 7
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: pSB2K3 is a variable copy number plasmid carrying kanamycin resistance. The plasmid contains the F' replication origin and also the P1 lytic replication origin. Genes encoding proteins that are required to activate the P1 lytic replication origin are carried on the plasmid under the control of a lacI-regulated promoter. When grown in cells producing LacI the plasmid is maintained at very low copy (<10 per cell). When induced with IPTG the P1 lytic replication proteins are produced and the P1 lytic replication origin takes over, raising the copy number to very high levels (>100++ per cell). The copy control features of pSB2K3 are useful for cloning "difficult" parts while still being able to prepare large amounts of DNA. Additionally, pSB2K3 has terminators bracketing its MCS which are designed to prevent transcription from *inside* the MCS from reading out into the vector. The efficiency of these terminators is known to be < 100%. Ideally we would construct a future set of terminators for bracketing a MCS that were 100% efficient in terminating both into and out of the MCS region.
mutable notes: *** pSCANS via BNL *** The pSB2 series plasmids are based on the pSCANS-5 plasmid from John Dunn's group at Brookhaven National Laboratory. The pSCANS plasmids are introduced here.
mutable provenance: MIT SBWG modification of pSCANS-5 from BNL
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: E. coli his operon terminator
sequence annotation: kanR
sequence annotation: repL
sequence annotation: repE (rev)
sequence annotation: terminator stem loop
sequence annotation: suffix
sequence annotation: VR
sequence annotation: lacOP
sequence annotation: end of terminator
sequence annotation: nnpp promoters
sequence annotation: deletion of two bases
sequence annotation: F1 Replication Origin
sequence annotation: VF2
sequence annotation: prefix
sequence annotation: oriS Replication Origin (rev)
sequence: https://synbiohub.org/public/igem/pSB2K3_sequence/1

E. coli his operon terminator
type: SequenceAnnotation
persistent identity: annotation1824015
display id: annotation1824015
version: 1
top level: pSB2K3
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB2K3/annotation1824015/range1824015/1

https://synbiohub.org/public/igem/pSB2K3/annotation1824015/range1824015/1
type: Range
persistent identity: range1824015
display id: range1824015
version: 1
direction: direction/forward
top level: pSB2K3
start: 22
end: 93
orientation: inline

kanR
type: SequenceAnnotation
persistent identity: annotation1023020
display id: annotation1023020
version: 1
top level: pSB2K3
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/pSB2K3/annotation1023020/range1023020/1

https://synbiohub.org/public/igem/pSB2K3/annotation1023020/range1023020/1
type: Range
persistent identity: range1023020
display id: range1023020
version: 1
direction: direction/forward
top level: pSB2K3
start: 3433
end: 4248
orientation: inline

repL
type: SequenceAnnotation
persistent identity: annotation1023027
display id: annotation1023027
version: 1
top level: pSB2K3
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/pSB2K3/annotation1023027/range1023027/1

https://synbiohub.org/public/igem/pSB2K3/annotation1023027/range1023027/1
type: Range
persistent identity: range1023027
display id: range1023027
version: 1
direction: direction/forward
top level: pSB2K3
start: 2289
end: 3134
orientation: inline

repE (rev)
type: SequenceAnnotation
persistent identity: annotation1023024
display id: annotation1023024
version: 1
top level: pSB2K3
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/pSB2K3/annotation1023024/range1023024/1

https://synbiohub.org/public/igem/pSB2K3/annotation1023024/range1023024/1
type: Range
persistent identity: range1023024
display id: range1023024
version: 1
direction: direction/reverse
top level: pSB2K3
start: 617
end: 1372
orientation: inline

terminator stem loop
type: SequenceAnnotation
persistent identity: annotation1824014
display id: annotation1824014
version: 1
top level: pSB2K3
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/pSB2K3/annotation1824014/range1824014/1

https://synbiohub.org/public/igem/pSB2K3/annotation1824014/range1824014/1
type: Range
persistent identity: range1824014
display id: range1824014
version: 1
direction: direction/forward
top level: pSB2K3
start: 30
end: 64
orientation: inline

suffix
type: SequenceAnnotation
persistent identity: annotation1023022
display id: annotation1023022
version: 1
top level: pSB2K3
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/pSB2K3/annotation1023022/range1023022/1

https://synbiohub.org/public/igem/pSB2K3/annotation1023022/range1023022/1
type: Range
persistent identity: range1023022
display id: range1023022
version: 1
direction: direction/forward
top level: pSB2K3
start: 1
end: 21
orientation: inline

VR
type: SequenceAnnotation
persistent identity: annotation1782483
display id: annotation1782483
version: 1
top level: pSB2K3
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/pSB2K3/annotation1782483/range1782483/1

https://synbiohub.org/public/igem/pSB2K3/annotation1782483/range1782483/1
type: Range
persistent identity: range1782483
display id: range1782483
version: 1
direction: direction/reverse
top level: pSB2K3
start: 157
end: 176
orientation: inline

lacOP
type: SequenceAnnotation
persistent identity: annotation1023026
display id: annotation1023026
version: 1
top level: pSB2K3
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/pSB2K3/annotation1023026/range1023026/1

https://synbiohub.org/public/igem/pSB2K3/annotation1023026/range1023026/1
type: Range
persistent identity: range1023026
display id: range1023026
version: 1
direction: direction/forward
top level: pSB2K3
start: 2037
end: 2211
orientation: inline

end of terminator
type: SequenceAnnotation
persistent identity: annotation1824017
display id: annotation1824017
version: 1
top level: pSB2K3
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB2K3/annotation1824017/range1824017/1

https://synbiohub.org/public/igem/pSB2K3/annotation1824017/range1824017/1
type: Range
persistent identity: range1824017
display id: range1824017
version: 1
direction: direction/reverse
top level: pSB2K3
start: 4362
end: 4365
orientation: inline

nnpp promoters
type: SequenceAnnotation
persistent identity: annotation1023028
display id: annotation1023028
version: 1
top level: pSB2K3
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/pSB2K3/annotation1023028/range1023028/1

https://synbiohub.org/public/igem/pSB2K3/annotation1023028/range1023028/1
type: Range
persistent identity: range1023028
display id: range1023028
version: 1
direction: direction/forward
top level: pSB2K3
start: 3242
end: 3393
orientation: inline

deletion of two bases
type: SequenceAnnotation
persistent identity: annotation1823781
display id: annotation1823781
version: 1
top level: pSB2K3
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/pSB2K3/annotation1823781/range1823781/1

https://synbiohub.org/public/igem/pSB2K3/annotation1823781/range1823781/1
type: Range
persistent identity: range1823781
display id: range1823781
version: 1
direction: direction/forward
top level: pSB2K3
start: 34
end: 35
orientation: inline

F1 Replication Origin
type: SequenceAnnotation
persistent identity: annotation1023023
display id: annotation1023023
version: 1
top level: pSB2K3
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/pSB2K3/annotation1023023/range1023023/1

https://synbiohub.org/public/igem/pSB2K3/annotation1023023/range1023023/1
type: Range
persistent identity: range1023023
display id: range1023023
version: 1
direction: direction/forward
top level: pSB2K3
start: 242
end: 290
orientation: inline

terminator stem loop
type: SequenceAnnotation
persistent identity: annotation1824016
display id: annotation1824016
version: 1
top level: pSB2K3
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/pSB2K3/annotation1824016/range1824016/1

https://synbiohub.org/public/igem/pSB2K3/annotation1824016/range1824016/1
type: Range
persistent identity: range1824016
display id: range1824016
version: 1
direction: direction/reverse
top level: pSB2K3
start: 4375
end: 4394
orientation: inline

VF2
type: SequenceAnnotation
persistent identity: annotation1782484
display id: annotation1782484
version: 1
top level: pSB2K3
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/pSB2K3/annotation1782484/range1782484/1

https://synbiohub.org/public/igem/pSB2K3/annotation1782484/range1782484/1
type: Range
persistent identity: range1782484
display id: range1782484
version: 1
direction: direction/forward
top level: pSB2K3
start: 4286
end: 4305
orientation: inline

prefix
type: SequenceAnnotation
persistent identity: annotation1023021
display id: annotation1023021
version: 1
top level: pSB2K3
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/pSB2K3/annotation1023021/range1023021/1

https://synbiohub.org/public/igem/pSB2K3/annotation1023021/range1023021/1
type: Range
persistent identity: range1023021
display id: range1023021
version: 1
direction: direction/forward
top level: pSB2K3
start: 4404
end: 4425
orientation: inline

oriS Replication Origin (rev)
type: SequenceAnnotation
persistent identity: annotation1023025
display id: annotation1023025
version: 1
top level: pSB2K3
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/pSB2K3/annotation1023025/range1023025/1

https://synbiohub.org/public/igem/pSB2K3/annotation1023025/range1023025/1
type: Range
persistent identity: range1023025
display id: range1023025
version: 1
direction: direction/reverse
top level: pSB2K3
start: 1463
end: 1679
orientation: inline

pSB8C15
type: ComponentDefinition
persistent identity: BBa K864017
display id: BBa_K864017
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Low copy BioBrick temperature sensitive standard vector
creator: Erik Lundin
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:37Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1124_
m user id: 0
owner id: 9827
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: pSB8C15 is a BioBrick standard vector with low copy pSC101ts replication origin (~5 copies per cell) and Chloramphenicol antibiotic resistance marker, especially usable for Lambda Red recombineering in E coli. The pSC101ts is a temperature sensitive pSC101 repelication origin and propagates at 30???C but stop replicating at 42???C. The backbone sequence is based on pSB3T5, but the E coli His operon terminator BBa_B0053 has been replaced with the late terminator of the Salmonella phage P22.
mutable notes: The resistance cassette is terminated by a Lux bidirectional terminator. Adjacent to this terminator there is a designed primer binding site to where the forward primer for lambda red can be designed to bind to no matter what the insert in the cloning site is or what the resistance is. The resistance cassette is flanked by SalI and SacI restriction sites for easy switching of resistance in the backbone. The pSC101ts origin of replication is flaked by NheI and MluI restriction sites for easy switching of origin of replication.
mutable provenance: Based on BBa_K864001
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: Lux terminator
sequence annotation: cmR
sequence annotation: tonB terminator
sequence annotation: T3 terminator
sequence annotation: Promoter
sequence annotation: -35
sequence annotation: VF2 forward primer binding site
sequence annotation: VR reverse primer binding site
sequence annotation: P22 late terminator
sequence annotation: pSC101-ts ori
sequence annotation: rrnC
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_K864017_sequence/1

Lux terminator
type: SequenceAnnotation
persistent identity: annotation2203159
display id: annotation2203159
version: 1
top level: pSB8C15
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864017/annotation2203159/range2203159/1

https://synbiohub.org/public/igem/BBa_K864017/annotation2203159/range2203159/1
type: Range
persistent identity: range2203159
display id: range2203159
version: 1
direction: direction/reverse
top level: pSB8C15
start: 2454
end: 2499
orientation: inline

cmR
type: SequenceAnnotation
persistent identity: annotation2203155
display id: annotation2203155
version: 1
top level: pSB8C15
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K864017/annotation2203155/range2203155/1

https://synbiohub.org/public/igem/BBa_K864017/annotation2203155/range2203155/1
type: Range
persistent identity: range2203155
display id: range2203155
version: 1
direction: direction/reverse
top level: pSB8C15
start: 2506
end: 3168
orientation: inline

tonB terminator
type: SequenceAnnotation
persistent identity: annotation2203157
display id: annotation2203157
version: 1
top level: pSB8C15
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K864017/annotation2203157/range2203157/1

https://synbiohub.org/public/igem/BBa_K864017/annotation2203157/range2203157/1
type: Range
persistent identity: range2203157
display id: range2203157
version: 1
direction: direction/forward
top level: pSB8C15
start: 47
end: 115
orientation: inline

T3 terminator
type: SequenceAnnotation
persistent identity: annotation2203161
display id: annotation2203161
version: 1
top level: pSB8C15
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864017/annotation2203161/range2203161/1

https://synbiohub.org/public/igem/BBa_K864017/annotation2203161/range2203161/1
type: Range
persistent identity: range2203161
display id: range2203161
version: 1
direction: direction/forward
top level: pSB8C15
start: 3342
end: 3419
orientation: inline

Promoter
type: SequenceAnnotation
persistent identity: annotation2202888
display id: annotation2202888
version: 1
top level: pSB8C15
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K864017/annotation2202888/range2202888/1

https://synbiohub.org/public/igem/BBa_K864017/annotation2202888/range2202888/1
type: Range
persistent identity: range2202888
display id: range2202888
version: 1
direction: direction/reverse
top level: pSB8C15
start: 3235
end: 3461
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2203156
display id: annotation2203156
version: 1
top level: pSB8C15
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864017/annotation2203156/range2203156/1

https://synbiohub.org/public/igem/BBa_K864017/annotation2203156/range2203156/1
type: Range
persistent identity: range2203156
display id: range2203156
version: 1
direction: direction/forward
top level: pSB8C15
start: 881
end: 886
orientation: inline

VF2 forward primer binding site
type: SequenceAnnotation
persistent identity: annotation2203154
display id: annotation2203154
version: 1
top level: pSB8C15
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K864017/annotation2203154/range2203154/1

https://synbiohub.org/public/igem/BBa_K864017/annotation2203154/range2203154/1
type: Range
persistent identity: range2203154
display id: range2203154
version: 1
direction: direction/forward
top level: pSB8C15
start: 3322
end: 3341
orientation: inline

VR reverse primer binding site
type: SequenceAnnotation
persistent identity: annotation2203046
display id: annotation2203046
version: 1
top level: pSB8C15
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K864017/annotation2203046/range2203046/1

https://synbiohub.org/public/igem/BBa_K864017/annotation2203046/range2203046/1
type: Range
persistent identity: range2203046
display id: range2203046
version: 1
direction: direction/reverse
top level: pSB8C15
start: 116
end: 135
orientation: inline

P22 late terminator
type: SequenceAnnotation
persistent identity: annotation2203160
display id: annotation2203160
version: 1
top level: pSB8C15
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864017/annotation2203160/range2203160/1

https://synbiohub.org/public/igem/BBa_K864017/annotation2203160/range2203160/1
type: Range
persistent identity: range2203160
display id: range2203160
version: 1
direction: direction/forward
top level: pSB8C15
start: 3281
end: 3321
orientation: inline

pSC101-ts ori
type: SequenceAnnotation
persistent identity: annotation2203153
display id: annotation2203153
version: 1
top level: pSB8C15
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864017/annotation2203153/range2203153/1

https://synbiohub.org/public/igem/BBa_K864017/annotation2203153/range2203153/1
type: Range
persistent identity: range2203153
display id: range2203153
version: 1
direction: direction/forward
top level: pSB8C15
start: 190
end: 2405
orientation: inline

rrnC
type: SequenceAnnotation
persistent identity: annotation2203158
display id: annotation2203158
version: 1
top level: pSB8C15
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864017/annotation2203158/range2203158/1

https://synbiohub.org/public/igem/BBa_K864017/annotation2203158/range2203158/1
type: Range
persistent identity: range2203158
display id: range2203158
version: 1
direction: direction/forward
top level: pSB8C15
start: 136
end: 175
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2203162
display id: annotation2203162
version: 1
top level: pSB8C15
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K864017/annotation2203162/range2203162/1

https://synbiohub.org/public/igem/BBa_K864017/annotation2203162/range2203162/1
type: Range
persistent identity: range2203162
display id: range2203162
version: 1
direction: direction/forward
top level: pSB8C15
start: 903
end: 908
orientation: inline

Transfecti
type: ComponentDefinition
persistent identity: BBa K157041
display id: BBa_K157041
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Transfection vector (similar to pSB1AK8)
creator: iGEM Team Freiburg 2008
created: 2008-10-25T11:00:00Z
modified: 2015-05-08T01:10:55Z
discontinued: false
dominant: false
experience: experience/None
group u list: _232_
m user id: 0
owner id: 1673
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: none
mutable notes: none
mutable provenance: none
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: BioBrick prefix (missing a 3' G)
sequence annotation: VF2
sequence annotation: kanamycin resistance marker
sequence annotation: Eukaryotic terminator
sequence annotation: rep (pMB1)
sequence annotation: BioBrick suffix (missing a C)
sequence annotation: ampicillin resistance marker
sequence annotation: Stem loop
sequence annotation: VR
sequence: https://synbiohub.org/public/igem/BBa_K157041_sequence/1

BioBrick prefix (missing a 3' G)
type: SequenceAnnotation
persistent identity: annotation2064659
display id: annotation2064659
version: 1
top level: Transfecti
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157041/annotation2064659/range2064659/1

https://synbiohub.org/public/igem/BBa_K157041/annotation2064659/range2064659/1
type: Range
persistent identity: range2064659
display id: range2064659
version: 1
direction: direction/forward
top level: Transfecti
start: 3405
end: 3425
orientation: inline

VF2
type: SequenceAnnotation
persistent identity: annotation2064658
display id: annotation2064658
version: 1
top level: Transfecti
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K157041/annotation2064658/range2064658/1

https://synbiohub.org/public/igem/BBa_K157041/annotation2064658/range2064658/1
type: Range
persistent identity: range2064658
display id: range2064658
version: 1
direction: direction/forward
top level: Transfecti
start: 3207
end: 3226
orientation: inline

kanamycin resistance marker
type: SequenceAnnotation
persistent identity: annotation2064656
display id: annotation2064656
version: 1
top level: Transfecti
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K157041/annotation2064656/range2064656/1

https://synbiohub.org/public/igem/BBa_K157041/annotation2064656/range2064656/1
type: Range
persistent identity: range2064656
display id: range2064656
version: 1
direction: direction/reverse
top level: Transfecti
start: 1334
end: 2149
orientation: inline

Eukaryotic terminator
type: SequenceAnnotation
persistent identity: annotation1992677
display id: annotation1992677
version: 1
top level: Transfecti
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K157041/annotation1992677/range1992677/1

https://synbiohub.org/public/igem/BBa_K157041/annotation1992677/range1992677/1
type: Range
persistent identity: range1992677
display id: range1992677
version: 1
direction: direction/forward
top level: Transfecti
start: 21
end: 257
orientation: inline

rep (pMB1)
type: SequenceAnnotation
persistent identity: annotation1992681
display id: annotation1992681
version: 1
top level: Transfecti
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157041/annotation1992681/range1992681/1

https://synbiohub.org/public/igem/BBa_K157041/annotation1992681/range1992681/1
type: Range
persistent identity: range1992681
display id: range1992681
version: 1
direction: direction/reverse
top level: Transfecti
start: 498
end: 1196
orientation: inline

BioBrick suffix (missing a C)
type: SequenceAnnotation
persistent identity: annotation1992678
display id: annotation1992678
version: 1
top level: Transfecti
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157041/annotation1992678/range1992678/1

https://synbiohub.org/public/igem/BBa_K157041/annotation1992678/range1992678/1
type: Range
persistent identity: range1992678
display id: range1992678
version: 1
direction: direction/forward
top level: Transfecti
start: 1
end: 21
orientation: inline

ampicillin resistance marker
type: SequenceAnnotation
persistent identity: annotation2064657
display id: annotation2064657
version: 1
top level: Transfecti
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K157041/annotation2064657/range2064657/1

https://synbiohub.org/public/igem/BBa_K157041/annotation2064657/range2064657/1
type: Range
persistent identity: range2064657
display id: range2064657
version: 1
direction: direction/reverse
top level: Transfecti
start: 2304
end: 3164
orientation: inline

Stem loop
type: SequenceAnnotation
persistent identity: annotation1992679
display id: annotation1992679
version: 1
top level: Transfecti
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K157041/annotation1992679/range1992679/1

https://synbiohub.org/public/igem/BBa_K157041/annotation1992679/range1992679/1
type: Range
persistent identity: range1992679
display id: range1992679
version: 1
direction: direction/forward
top level: Transfecti
start: 267
end: 301
orientation: inline

VR
type: SequenceAnnotation
persistent identity: annotation1992680
display id: annotation1992680
version: 1
top level: Transfecti
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K157041/annotation1992680/range1992680/1

https://synbiohub.org/public/igem/BBa_K157041/annotation1992680/range1992680/1
type: Range
persistent identity: range1992680
display id: range1992680
version: 1
direction: direction/reverse
top level: Transfecti
start: 394
end: 413
orientation: inline

BAC
type: ComponentDefinition
persistent identity: BBa J61031
display id: BBa_J61031
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Amplifiable BAC plasmid
creator: John Anderson
created: 2006-09-20T11:00:00Z
modified: 2015-08-31T02:02:59Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This plasmid is derived originally from pBeloBAC11 with multiple modifications to make it compatible with biobricks and more useable. Without modification, J61031 is a high-copy plasmid in normal laboratory strains of E. coli due to the colE1 origin of replication located within the biobrick part region. It also confers ampicillin resistance, kanamycin resistance, and constitutively-expressed RFP. Upon replacement of the SpeI/XbaI region, the colE1, ampR, and RFP features are removed, leaving behind the F plasmid replicon, the KanR gene, and an R6K origin of replication. At this point, the plasmid will replicate as a medium or high copy plasmid in pir+ or pir116 strains such as Ec100D::pir116. In non-pir strains (most lab strains), the plasmid will replicate at near-single copy levels. The restriction sites EcoRI, SpeI, NotI, and XbaI are all unique in this plasmid. There are several PstI sites, however. pBACr-NESd
mutable notes: N/A
mutable provenance: many many steps
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: finO incD/sopC region; contains 12 43 bp direct repeats that bind SopB protein; required for partitioning of F plasmid to daughter cells after replication as well as repression of sopAB operator;
sequence annotation: pBR322 origin
sequence annotation: kanamycin/neomycin resistance marker (NTP II marker)
sequence annotation: PstI
sequence annotation: SpeI
sequence annotation: GFP reporter
sequence annotation: beta-lactamase ampicillin resistance marker
sequence annotation: c myc tag
sequence annotation: beta-lactamase (ampR) promoter
sequence annotation: pMB1 replication origin
sequence annotation: NotI
sequence annotation: sopB
sequence annotation: R6Kg origin
sequence annotation: kanR/neoR promoter
sequence annotation: rrnB T1 terminator
sequence annotation: mRFP
sequence annotation: EcoRI
sequence annotation: BBa_R0040 Ptet promoter
sequence annotation: 6xHis tag
sequence annotation: sopA
sequence annotation: repE
sequence annotation: rrnB terminator
sequence: https://synbiohub.org/public/igem/BBa_J61031_sequence/1

finO incD/sopC region; contains 12 43 bp direct repeats that bind SopB protein; required for partitioning of F plasmid to daughter cells after replication as well as repression of sopAB operator;
type: SequenceAnnotation
persistent identity: annotation1976020
display id: annotation1976020
version: 1
top level: BAC
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J61031/annotation1976020/range1976020/1

https://synbiohub.org/public/igem/BBa_J61031/annotation1976020/range1976020/1
type: Range
persistent identity: range1976020
display id: range1976020
version: 1
direction: direction/reverse
top level: BAC
start: 145
end: 618
orientation: inline

pBR322 origin
type: SequenceAnnotation
persistent identity: annotation1934582
display id: annotation1934582
version: 1
top level: BAC
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_J61031/annotation1934582/range1934582/1

https://synbiohub.org/public/igem/BBa_J61031/annotation1934582/range1934582/1
type: Range
persistent identity: range1934582
display id: range1934582
version: 1
direction: direction/forward
top level: BAC
start: 7864
end: 8303
orientation: inline

kanamycin/neomycin resistance marker (NTP II marker)
type: SequenceAnnotation
persistent identity: annotation1934584
display id: annotation1934584
version: 1
top level: BAC
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J61031/annotation1934584/range1934584/1

https://synbiohub.org/public/igem/BBa_J61031/annotation1934584/range1934584/1
type: Range
persistent identity: range1934584
display id: range1934584
version: 1
direction: direction/forward
top level: BAC
start: 4891
end: 5685
orientation: inline

PstI
type: SequenceAnnotation
persistent identity: annotation1976016
display id: annotation1976016
version: 1
top level: BAC
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_J61031/annotation1976016/range1976016/1

https://synbiohub.org/public/igem/BBa_J61031/annotation1976016/range1976016/1
type: Range
persistent identity: range1976016
display id: range1976016
version: 1
direction: direction/forward
top level: BAC
start: 5068
end: 5073
orientation: inline

SpeI
type: SequenceAnnotation
persistent identity: annotation1976012
display id: annotation1976012
version: 1
top level: BAC
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_J61031/annotation1976012/range1976012/1

https://synbiohub.org/public/igem/BBa_J61031/annotation1976012/range1976012/1
type: Range
persistent identity: range1976012
display id: range1976012
version: 1
direction: direction/forward
top level: BAC
start: 2
end: 7
orientation: inline

GFP reporter
type: SequenceAnnotation
persistent identity: annotation1976009
display id: annotation1976009
version: 1
top level: BAC
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J61031/annotation1976009/range1976009/1

https://synbiohub.org/public/igem/BBa_J61031/annotation1976009/range1976009/1
type: Range
persistent identity: range1976009
display id: range1976009
version: 1
direction: direction/reverse
top level: BAC
start: 8985
end: 9698
orientation: inline

beta-lactamase ampicillin resistance marker
type: SequenceAnnotation
persistent identity: annotation1976008
display id: annotation1976008
version: 1
top level: BAC
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J61031/annotation1976008/range1976008/1

https://synbiohub.org/public/igem/BBa_J61031/annotation1976008/range1976008/1
type: Range
persistent identity: range1976008
display id: range1976008
version: 1
direction: direction/forward
top level: BAC
start: 6669
end: 7529
orientation: inline

c myc tag
type: SequenceAnnotation
persistent identity: annotation1976006
display id: annotation1976006
version: 1
top level: BAC
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_J61031/annotation1976006/range1976006/1

https://synbiohub.org/public/igem/BBa_J61031/annotation1976006/range1976006/1
type: Range
persistent identity: range1976006
display id: range1976006
version: 1
direction: direction/reverse
top level: BAC
start: 6484
end: 6513
orientation: inline

beta-lactamase (ampR) promoter
type: SequenceAnnotation
persistent identity: annotation1976007
display id: annotation1976007
version: 1
top level: BAC
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J61031/annotation1976007/range1976007/1

https://synbiohub.org/public/igem/BBa_J61031/annotation1976007/range1976007/1
type: Range
persistent identity: range1976007
display id: range1976007
version: 1
direction: direction/forward
top level: BAC
start: 6599
end: 6627
orientation: inline

pMB1 replication origin
type: SequenceAnnotation
persistent identity: annotation1934583
display id: annotation1934583
version: 1
top level: BAC
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_J61031/annotation1934583/range1934583/1

https://synbiohub.org/public/igem/BBa_J61031/annotation1934583/range1934583/1
type: Range
persistent identity: range1934583
display id: range1934583
version: 1
direction: direction/reverse
top level: BAC
start: 7691
end: 8303
orientation: inline

NotI
type: SequenceAnnotation
persistent identity: annotation1976013
display id: annotation1976013
version: 1
top level: BAC
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_J61031/annotation1976013/range1976013/1

https://synbiohub.org/public/igem/BBa_J61031/annotation1976013/range1976013/1
type: Range
persistent identity: range1976013
display id: range1976013
version: 1
direction: direction/forward
top level: BAC
start: 9
end: 16
orientation: inline

sopB
type: SequenceAnnotation
persistent identity: annotation1975998
display id: annotation1975998
version: 1
top level: BAC
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J61031/annotation1975998/range1975998/1

https://synbiohub.org/public/igem/BBa_J61031/annotation1975998/range1975998/1
type: Range
persistent identity: range1975998
display id: range1975998
version: 1
direction: direction/reverse
top level: BAC
start: 691
end: 1662
orientation: inline

R6Kg origin
type: SequenceAnnotation
persistent identity: annotation1976002
display id: annotation1976002
version: 1
top level: BAC
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_J61031/annotation1976002/range1976002/1

https://synbiohub.org/public/igem/BBa_J61031/annotation1976002/range1976002/1
type: Range
persistent identity: range1976002
display id: range1976002
version: 1
direction: direction/reverse
top level: BAC
start: 5743
end: 6126
orientation: inline

kanR/neoR promoter
type: SequenceAnnotation
persistent identity: annotation1976001
display id: annotation1976001
version: 1
top level: BAC
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J61031/annotation1976001/range1976001/1

https://synbiohub.org/public/igem/BBa_J61031/annotation1976001/range1976001/1
type: Range
persistent identity: range1976001
display id: range1976001
version: 1
direction: direction/forward
top level: BAC
start: 4753
end: 4802
orientation: inline

rrnB T1 terminator
type: SequenceAnnotation
persistent identity: annotation1976003
display id: annotation1976003
version: 1
top level: BAC
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_J61031/annotation1976003/range1976003/1

https://synbiohub.org/public/igem/BBa_J61031/annotation1976003/range1976003/1
type: Range
persistent identity: range1976003
display id: range1976003
version: 1
direction: direction/reverse
top level: BAC
start: 6179
end: 6222
orientation: inline

PstI
type: SequenceAnnotation
persistent identity: annotation1976018
display id: annotation1976018
version: 1
top level: BAC
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_J61031/annotation1976018/range1976018/1

https://synbiohub.org/public/igem/BBa_J61031/annotation1976018/range1976018/1
type: Range
persistent identity: range1976018
display id: range1976018
version: 1
direction: direction/forward
top level: BAC
start: 8975
end: 8980
orientation: inline

rrnB T1 terminator
type: SequenceAnnotation
persistent identity: annotation1976011
display id: annotation1976011
version: 1
top level: BAC
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_J61031/annotation1976011/range1976011/1

https://synbiohub.org/public/igem/BBa_J61031/annotation1976011/range1976011/1
type: Range
persistent identity: range1976011
display id: range1976011
version: 1
direction: direction/forward
top level: BAC
start: 10540
end: 10583
orientation: inline

PstI
type: SequenceAnnotation
persistent identity: annotation1976014
display id: annotation1976014
version: 1
top level: BAC
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_J61031/annotation1976014/range1976014/1

https://synbiohub.org/public/igem/BBa_J61031/annotation1976014/range1976014/1
type: Range
persistent identity: range1976014
display id: range1976014
version: 1
direction: direction/forward
top level: BAC
start: 2001
end: 2006
orientation: inline

mRFP
type: SequenceAnnotation
persistent identity: annotation1976010
display id: annotation1976010
version: 1
top level: BAC
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J61031/annotation1976010/range1976010/1

https://synbiohub.org/public/igem/BBa_J61031/annotation1976010/range1976010/1
type: Range
persistent identity: range1976010
display id: range1976010
version: 1
direction: direction/forward
top level: BAC
start: 9815
end: 10495
orientation: inline

EcoRI
type: SequenceAnnotation
persistent identity: annotation1976017
display id: annotation1976017
version: 1
top level: BAC
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_J61031/annotation1976017/range1976017/1

https://synbiohub.org/public/igem/BBa_J61031/annotation1976017/range1976017/1
type: Range
persistent identity: range1976017
display id: range1976017
version: 1
direction: direction/forward
top level: BAC
start: 6527
end: 6532
orientation: inline

BBa_R0040 Ptet promoter
type: SequenceAnnotation
persistent identity: annotation1976021
display id: annotation1976021
version: 1
top level: BAC
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J61031/annotation1976021/range1976021/1

https://synbiohub.org/public/igem/BBa_J61031/annotation1976021/range1976021/1
type: Range
persistent identity: range1976021
display id: range1976021
version: 1
direction: direction/forward
top level: BAC
start: 9735
end: 9788
orientation: inline

PstI
type: SequenceAnnotation
persistent identity: annotation1976015
display id: annotation1976015
version: 1
top level: BAC
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_J61031/annotation1976015/range1976015/1

https://synbiohub.org/public/igem/BBa_J61031/annotation1976015/range1976015/1
type: Range
persistent identity: range1976015
display id: range1976015
version: 1
direction: direction/forward
top level: BAC
start: 3542
end: 3547
orientation: inline

6xHis tag
type: SequenceAnnotation
persistent identity: annotation1976005
display id: annotation1976005
version: 1
top level: BAC
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_J61031/annotation1976005/range1976005/1

https://synbiohub.org/public/igem/BBa_J61031/annotation1976005/range1976005/1
type: Range
persistent identity: range1976005
display id: range1976005
version: 1
direction: direction/reverse
top level: BAC
start: 6451
end: 6468
orientation: inline

sopA
type: SequenceAnnotation
persistent identity: annotation1975999
display id: annotation1975999
version: 1
top level: BAC
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J61031/annotation1975999/range1975999/1

https://synbiohub.org/public/igem/BBa_J61031/annotation1975999/range1975999/1
type: Range
persistent identity: range1975999
display id: range1975999
version: 1
direction: direction/reverse
top level: BAC
start: 1662
end: 2837
orientation: inline

repE
type: SequenceAnnotation
persistent identity: annotation1976000
display id: annotation1976000
version: 1
top level: BAC
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J61031/annotation1976000/range1976000/1

https://synbiohub.org/public/igem/BBa_J61031/annotation1976000/range1976000/1
type: Range
persistent identity: range1976000
display id: range1976000
version: 1
direction: direction/reverse
top level: BAC
start: 3416
end: 4171
orientation: inline

rrnB terminator
type: SequenceAnnotation
persistent identity: annotation1976004
display id: annotation1976004
version: 1
top level: BAC
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_J61031/annotation1976004/range1976004/1

https://synbiohub.org/public/igem/BBa_J61031/annotation1976004/range1976004/1
type: Range
persistent identity: range1976004
display id: range1976004
version: 1
direction: direction/reverse
top level: BAC
start: 6188
end: 6345
orientation: inline

BBa_K106698
type: ComponentDefinition
persistent identity: BBa K106698
display id: BBa_K106698
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: AarI AD acceptor vector (pRS315, 8X LexAOps Fig1P, Adh1t)
creator: Andrew Horwitz
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:09:00Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _226_
m user id: 0
owner id: 2659
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: Acceptor for AarI AD parts, with 8X LexA operators directly upstream of the medium strong Cyc1 promoter.
mutable notes: LexA Ops were cloned non-directionally into the PspOMI site. Inner site is broken, thus the cassette can be subcloned from this plasmid by PspOMI/SacI digestion (for example, to change markers).
mutable provenance: Modification of the AarI AD acceptor vector (pRS315, Cyc1P, Adh1t).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: lac promoter
sequence annotation: A overhang
sequence annotation: ampicillin marker promoter
sequence annotation: Stop codons in all 3 frames
sequence annotation: 8X LexA Operators
sequence annotation: LEU2 marker
sequence annotation: lacZalpha reporter
sequence annotation: T3 promoter
sequence annotation: D overhang
sequence annotation: pBR322 origin
sequence annotation: f1 origin
sequence annotation: ampicillin resistance marker
sequence annotation: CEN ARS
sequence annotation: Adh1 terminator
sequence annotation: Fig1 Promoter
sequence annotation: T7 promoter
sequence: https://synbiohub.org/public/igem/BBa_K106698_sequence/1

lac promoter
type: SequenceAnnotation
persistent identity: annotation1999743
display id: annotation1999743
version: 1
top level: BBa_K106698
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K106698/annotation1999743/range1999743/1

https://synbiohub.org/public/igem/BBa_K106698/annotation1999743/range1999743/1
type: Range
persistent identity: range1999743
display id: range1999743
version: 1
direction: direction/forward
top level: BBa_K106698
start: 5996
end: 6025
orientation: inline

A overhang
type: SequenceAnnotation
persistent identity: annotation1999747
display id: annotation1999747
version: 1
top level: BBa_K106698
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K106698/annotation1999747/range1999747/1

https://synbiohub.org/public/igem/BBa_K106698/annotation1999747/range1999747/1
type: Range
persistent identity: range1999747
display id: range1999747
version: 1
direction: direction/forward
top level: BBa_K106698
start: 7309
end: 7312
orientation: inline

ampicillin marker promoter
type: SequenceAnnotation
persistent identity: annotation1999741
display id: annotation1999741
version: 1
top level: BBa_K106698
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K106698/annotation1999741/range1999741/1

https://synbiohub.org/public/igem/BBa_K106698/annotation1999741/range1999741/1
type: Range
persistent identity: range1999741
display id: range1999741
version: 1
direction: direction/forward
top level: BBa_K106698
start: 2983
end: 4011
orientation: inline

Stop codons in all 3 frames
type: SequenceAnnotation
persistent identity: annotation1999746
display id: annotation1999746
version: 1
top level: BBa_K106698
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K106698/annotation1999746/range1999746/1

https://synbiohub.org/public/igem/BBa_K106698/annotation1999746/range1999746/1
type: Range
persistent identity: range1999746
display id: range1999746
version: 1
direction: direction/forward
top level: BBa_K106698
start: 11
end: 21
orientation: inline

8X LexA Operators
type: SequenceAnnotation
persistent identity: annotation1995345
display id: annotation1995345
version: 1
top level: BBa_K106698
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K106698/annotation1995345/range1995345/1

https://synbiohub.org/public/igem/BBa_K106698/annotation1995345/range1995345/1
type: Range
persistent identity: range1995345
display id: range1995345
version: 1
direction: direction/forward
top level: BBa_K106698
start: 6132
end: 6299
orientation: inline

LEU2 marker
type: SequenceAnnotation
persistent identity: annotation1995348
display id: annotation1995348
version: 1
top level: BBa_K106698
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K106698/annotation1995348/range1995348/1

https://synbiohub.org/public/igem/BBa_K106698/annotation1995348/range1995348/1
type: Range
persistent identity: range1995348
display id: range1995348
version: 1
direction: direction/forward
top level: BBa_K106698
start: 1570
end: 2676
orientation: inline

lacZalpha reporter
type: SequenceAnnotation
persistent identity: annotation1999739
display id: annotation1999739
version: 1
top level: BBa_K106698
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K106698/annotation1999739/range1999739/1

https://synbiohub.org/public/igem/BBa_K106698/annotation1999739/range1999739/1
type: Range
persistent identity: range1999739
display id: range1999739
version: 1
direction: direction/forward
top level: BBa_K106698
start: 246
end: 405
orientation: inline

T3 promoter
type: SequenceAnnotation
persistent identity: annotation1999744
display id: annotation1999744
version: 1
top level: BBa_K106698
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K106698/annotation1999744/range1999744/1

https://synbiohub.org/public/igem/BBa_K106698/annotation1999744/range1999744/1
type: Range
persistent identity: range1999744
display id: range1999744
version: 1
direction: direction/forward
top level: BBa_K106698
start: 6095
end: 6114
orientation: inline

D overhang
type: SequenceAnnotation
persistent identity: annotation1999745
display id: annotation1999745
version: 1
top level: BBa_K106698
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K106698/annotation1999745/range1999745/1

https://synbiohub.org/public/igem/BBa_K106698/annotation1999745/range1999745/1
type: Range
persistent identity: range1999745
display id: range1999745
version: 1
direction: direction/forward
top level: BBa_K106698
start: 1
end: 4
orientation: inline

pBR322 origin
type: SequenceAnnotation
persistent identity: annotation1999742
display id: annotation1999742
version: 1
top level: BBa_K106698
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K106698/annotation1999742/range1999742/1

https://synbiohub.org/public/igem/BBa_K106698/annotation1999742/range1999742/1
type: Range
persistent identity: range1999742
display id: range1999742
version: 1
direction: direction/forward
top level: BBa_K106698
start: 5068
end: 5687
orientation: inline

f1 origin
type: SequenceAnnotation
persistent identity: annotation1999740
display id: annotation1999740
version: 1
top level: BBa_K106698
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K106698/annotation1999740/range1999740/1

https://synbiohub.org/public/igem/BBa_K106698/annotation1999740/range1999740/1
type: Range
persistent identity: range1999740
display id: range1999740
version: 1
direction: direction/forward
top level: BBa_K106698
start: 427
end: 733
orientation: inline

ampicillin resistance marker
type: SequenceAnnotation
persistent identity: annotation1995344
display id: annotation1995344
version: 1
top level: BBa_K106698
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K106698/annotation1995344/range1995344/1

https://synbiohub.org/public/igem/BBa_K106698/annotation1995344/range1995344/1
type: Range
persistent identity: range1995344
display id: range1995344
version: 1
direction: direction/forward
top level: BBa_K106698
start: 4053
end: 4913
orientation: inline

CEN ARS
type: SequenceAnnotation
persistent identity: annotation1995343
display id: annotation1995343
version: 1
top level: BBa_K106698
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K106698/annotation1995343/range1995343/1

https://synbiohub.org/public/igem/BBa_K106698/annotation1995343/range1995343/1
type: Range
persistent identity: range1995343
display id: range1995343
version: 1
direction: direction/reverse
top level: BBa_K106698
start: 3403
end: 3921
orientation: inline

Adh1 terminator
type: SequenceAnnotation
persistent identity: annotation1995347
display id: annotation1995347
version: 1
top level: BBa_K106698
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K106698/annotation1995347/range1995347/1

https://synbiohub.org/public/igem/BBa_K106698/annotation1995347/range1995347/1
type: Range
persistent identity: range1995347
display id: range1995347
version: 1
direction: direction/forward
top level: BBa_K106698
start: 22
end: 215
orientation: inline

Fig1 Promoter
type: SequenceAnnotation
persistent identity: annotation1995346
display id: annotation1995346
version: 1
top level: BBa_K106698
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K106698/annotation1995346/range1995346/1

https://synbiohub.org/public/igem/BBa_K106698/annotation1995346/range1995346/1
type: Range
persistent identity: range1995346
display id: range1995346
version: 1
direction: direction/forward
top level: BBa_K106698
start: 6300
end: 7309
orientation: inline

T7 promoter
type: SequenceAnnotation
persistent identity: annotation1999738
display id: annotation1999738
version: 1
top level: BBa_K106698
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K106698/annotation1999738/range1999738/1

https://synbiohub.org/public/igem/BBa_K106698/annotation1999738/range1999738/1
type: Range
persistent identity: range1999738
display id: range1999738
version: 1
direction: direction/reverse
top level: BBa_K106698
start: 224
end: 242
orientation: inline

pSBBs2E
type: ComponentDefinition
persistent identity: BBa K823027
display id: BBa_K823027
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pSBBs2E: Empty backbone for integration into Bacillus subtilis lacA locus
creator: Jara Radeck
created: 2012-08-20T11:00:00Z
modified: 2015-05-08T01:13:30Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1081_
m user id: 0
owner id: 11555
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This part is an empty backbone vector for the usage in Bacillus subtilis. It integrates in the lacA locus and can be selected with erythromycin + lincomycin (MLS). It has a ampicillin resistance for cloning in E.coli. This BioBrick is part of the LMU 2012 Bacillus BioBrick Box project: http://2012.igem.org/Team:LMU-Munich/Bacillus_BioBricks
mutable notes: We removed any forbidden restriction sites by site-directed mutagenesis PCR. The clonging site also includes a rfp for selection in E.coli.
mutable provenance: This part is an igem compatible version of the Bacillus subtilis vector pAX01
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: erm
sequence annotation: bla
sequence annotation: lacA'
sequence annotation: 'lacA
sequence: https://synbiohub.org/public/igem/BBa_K823027_sequence/1

erm
type: SequenceAnnotation
persistent identity: annotation2180263
display id: annotation2180263
version: 1
top level: pSBBs2E
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K823027/annotation2180263/range2180263/1

https://synbiohub.org/public/igem/BBa_K823027/annotation2180263/range2180263/1
type: Range
persistent identity: range2180263
display id: range2180263
version: 1
direction: direction/forward
top level: pSBBs2E
start: 500
end: 1300
orientation: inline

bla
type: SequenceAnnotation
persistent identity: annotation2180265
display id: annotation2180265
version: 1
top level: pSBBs2E
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K823027/annotation2180265/range2180265/1

https://synbiohub.org/public/igem/BBa_K823027/annotation2180265/range2180265/1
type: Range
persistent identity: range2180265
display id: range2180265
version: 1
direction: direction/reverse
top level: pSBBs2E
start: 4603
end: 5400
orientation: inline

lacA'
type: SequenceAnnotation
persistent identity: annotation2180264
display id: annotation2180264
version: 1
top level: pSBBs2E
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K823027/annotation2180264/range2180264/1

https://synbiohub.org/public/igem/BBa_K823027/annotation2180264/range2180264/1
type: Range
persistent identity: range2180264
display id: range2180264
version: 1
direction: direction/reverse
top level: pSBBs2E
start: 1600
end: 2100
orientation: inline

'lacA
type: SequenceAnnotation
persistent identity: annotation2180266
display id: annotation2180266
version: 1
top level: pSBBs2E
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K823027/annotation2180266/range2180266/1

https://synbiohub.org/public/igem/BBa_K823027/annotation2180266/range2180266/1
type: Range
persistent identity: range2180266
display id: range2180266
version: 1
direction: direction/reverse
top level: pSBBs2E
start: 5600
end: 6100
orientation: inline

pSB8K15
type: ComponentDefinition
persistent identity: BBa K864018
display id: BBa_K864018
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Low copy BioBrick temperature sensitive standard vector
creator: Erik Lundin
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:37Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1124_
m user id: 0
owner id: 9827
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: pSB8K15 is a BioBrick standard vector with low copy pSC101ts replication origin (~5 copies per cell) and ampicillin antibiotic resistance marker, especially usable for Lambda Red recombineering in E coli. The pSC101ts is a temperature sensitive pSC101 repelication origin and propagates at 30???C but stop replicating at 42???C. The backbone sequence is based on pSB3T5, but the E coli His operon terminator BBa_B0053 has been replaced with the late terminator of the Salmonella phage P22.
mutable notes: The resistance cassette is terminated by a Lux bidirectional terminator. Adjacent to this terminator there is a designed primer binding site to where the forward primer for lambda red can be designed to bind to no matter what the insert in the cloning site is or what the resistance is. The resistance cassette is flanked by SalI and SacI restriction sites for easy switching of resistance in the backbone. The pSC101ts origin of replication is flaked by NheI and MluI restriction sites for easy switching of origin of replication.
mutable provenance: Based on BBa_K864002
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence: https://synbiohub.org/public/igem/BBa_K864018_sequence/1

pSBBs1C
type: ComponentDefinition
persistent identity: BBa K823023
display id: BBa_K823023
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pSBBs1C: Empty backbone for integration into Bacillus subtilis amyE locus
creator: Jara Radeck
created: 2012-08-20T11:00:00Z
modified: 2015-05-08T01:13:30Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1081_
m user id: 0
owner id: 11555
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This part is an empty backbone vector for the usage in Bacillus subtilis. It integrates in the amyE locus and can be selected with Chloramphenicol (cat gene). It has a ampicillin resistance for cloning in E.coli. It originates from the Bacillus vector pDG1662. This BioBrick is part of the LMU 2012 Bacillus BioBrick Box project: http://2012.igem.org/Team:LMU-Munich/Bacillus_BioBricks
mutable notes: We removed any forbidden restriction sites by site-directed mutagenesis PCR.
mutable provenance: This backbone is an igem compatible version of the Bacillus vector pDG1662
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: amyE
sequence annotation: cat
sequence annotation: bla
sequence: https://synbiohub.org/public/igem/BBa_K823023_sequence/1

amyE
type: SequenceAnnotation
persistent identity: annotation2180237
display id: annotation2180237
version: 1
top level: pSBBs1C
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K823023/annotation2180237/range2180237/1

https://synbiohub.org/public/igem/BBa_K823023/annotation2180237/range2180237/1
type: Range
persistent identity: range2180237
display id: range2180237
version: 1
direction: direction/forward
top level: pSBBs1C
start: 1379
end: 2407
orientation: inline

amyE
type: SequenceAnnotation
persistent identity: annotation2180239
display id: annotation2180239
version: 1
top level: pSBBs1C
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K823023/annotation2180239/range2180239/1

https://synbiohub.org/public/igem/BBa_K823023/annotation2180239/range2180239/1
type: Range
persistent identity: range2180239
display id: range2180239
version: 1
direction: direction/forward
top level: pSBBs1C
start: 5546
end: 6066
orientation: inline

cat
type: SequenceAnnotation
persistent identity: annotation2180236
display id: annotation2180236
version: 1
top level: pSBBs1C
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K823023/annotation2180236/range2180236/1

https://synbiohub.org/public/igem/BBa_K823023/annotation2180236/range2180236/1
type: Range
persistent identity: range2180236
display id: range2180236
version: 1
direction: direction/forward
top level: pSBBs1C
start: 358
end: 1008
orientation: inline

bla
type: SequenceAnnotation
persistent identity: annotation2180238
display id: annotation2180238
version: 1
top level: pSBBs1C
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K823023/annotation2180238/range2180238/1

https://synbiohub.org/public/igem/BBa_K823023/annotation2180238/range2180238/1
type: Range
persistent identity: range2180238
display id: range2180238
version: 1
direction: direction/reverse
top level: pSBBs1C
start: 4361
end: 5221
orientation: inline

BBa_K106672
type: ComponentDefinition
persistent identity: BBa K106672
display id: BBa_K106672
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: AarI AD acceptor vector (pRS305, Gal1P, Adh1t)
creator: Andrew Horwitz
created: 2008-10-28T12:00:00Z
modified: 2015-05-08T01:08:59Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _226_
m user id: 0
owner id: 2659
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: This is an acceptor vector for AarI cloning, with A and D ends. Cloning into this plasmid will put your parts under the control of a Gal1 promoter (Gal1P) and Adh1 terminator (Adh1t). For more information regarding AarI acceptor vectors, and AarI cloning in general, please visit the UCSF 2008 wiki.
mutable notes: The pRS305 vector has been modified with an AarI cloning cassette inserted between the PspOMI and SacI sites. Please note, the actual orientation of this vector is reversed.
mutable provenance: A modified pRS305 plasmid--Integrating yeast expression vector (LEU2 marker).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: Gal1 promoter
sequence annotation: Adh1 terminator
sequence annotation: Start codon
sequence annotation: LEU2 marker
sequence annotation: f1 origin
sequence annotation: lacZalpha reporter
sequence annotation: lac promoter
sequence annotation: ampicillin resistance marker
sequence annotation: pBR322 origin
sequence annotation: T7 promoter
sequence annotation: Stop codons in all 3 frames
sequence annotation: T3 promoter
sequence annotation: A overhang
sequence annotation: ampicillin resistance promoter
sequence annotation: D overhang
sequence annotation: Gal1 Promoter
sequence: https://synbiohub.org/public/igem/BBa_K106672_sequence/1

Gal1 promoter
type: SequenceAnnotation
persistent identity: annotation1999712
display id: annotation1999712
version: 1
top level: BBa_K106672
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K106672/annotation1999712/range1999712/1

https://synbiohub.org/public/igem/BBa_K106672/annotation1999712/range1999712/1
type: Range
persistent identity: range1999712
display id: range1999712
version: 1
direction: direction/forward
top level: BBa_K106672
start: 6172
end: 6621
orientation: inline

Adh1 terminator
type: SequenceAnnotation
persistent identity: annotation1993688
display id: annotation1993688
version: 1
top level: BBa_K106672
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K106672/annotation1993688/range1993688/1

https://synbiohub.org/public/igem/BBa_K106672/annotation1993688/range1993688/1
type: Range
persistent identity: range1993688
display id: range1993688
version: 1
direction: direction/forward
top level: BBa_K106672
start: 30
end: 215
orientation: inline

Start codon
type: SequenceAnnotation
persistent identity: annotation1999714
display id: annotation1999714
version: 1
top level: BBa_K106672
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K106672/annotation1999714/range1999714/1

https://synbiohub.org/public/igem/BBa_K106672/annotation1999714/range1999714/1
type: Range
persistent identity: range1999714
display id: range1999714
version: 1
direction: direction/forward
top level: BBa_K106672
start: 6626
end: 6628
orientation: inline

LEU2 marker
type: SequenceAnnotation
persistent identity: annotation1993684
display id: annotation1993684
version: 1
top level: BBa_K106672
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K106672/annotation1993684/range1993684/1

https://synbiohub.org/public/igem/BBa_K106672/annotation1993684/range1993684/1
type: Range
persistent identity: range1993684
display id: range1993684
version: 1
direction: direction/forward
top level: BBa_K106672
start: 1570
end: 2676
orientation: inline

f1 origin
type: SequenceAnnotation
persistent identity: annotation1999707
display id: annotation1999707
version: 1
top level: BBa_K106672
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K106672/annotation1999707/range1999707/1

https://synbiohub.org/public/igem/BBa_K106672/annotation1999707/range1999707/1
type: Range
persistent identity: range1999707
display id: range1999707
version: 1
direction: direction/forward
top level: BBa_K106672
start: 427
end: 733
orientation: inline

lacZalpha reporter
type: SequenceAnnotation
persistent identity: annotation1999706
display id: annotation1999706
version: 1
top level: BBa_K106672
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K106672/annotation1999706/range1999706/1

https://synbiohub.org/public/igem/BBa_K106672/annotation1999706/range1999706/1
type: Range
persistent identity: range1999706
display id: range1999706
version: 1
direction: direction/forward
top level: BBa_K106672
start: 246
end: 405
orientation: inline

lac promoter
type: SequenceAnnotation
persistent identity: annotation1999710
display id: annotation1999710
version: 1
top level: BBa_K106672
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K106672/annotation1999710/range1999710/1

https://synbiohub.org/public/igem/BBa_K106672/annotation1999710/range1999710/1
type: Range
persistent identity: range1999710
display id: range1999710
version: 1
direction: direction/forward
top level: BBa_K106672
start: 5482
end: 5511
orientation: inline

ampicillin resistance marker
type: SequenceAnnotation
persistent identity: annotation1993685
display id: annotation1993685
version: 1
top level: BBa_K106672
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K106672/annotation1993685/range1993685/1

https://synbiohub.org/public/igem/BBa_K106672/annotation1993685/range1993685/1
type: Range
persistent identity: range1993685
display id: range1993685
version: 1
direction: direction/forward
top level: BBa_K106672
start: 3539
end: 4399
orientation: inline

pBR322 origin
type: SequenceAnnotation
persistent identity: annotation1999709
display id: annotation1999709
version: 1
top level: BBa_K106672
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K106672/annotation1999709/range1999709/1

https://synbiohub.org/public/igem/BBa_K106672/annotation1999709/range1999709/1
type: Range
persistent identity: range1999709
display id: range1999709
version: 1
direction: direction/forward
top level: BBa_K106672
start: 4554
end: 5173
orientation: inline

T7 promoter
type: SequenceAnnotation
persistent identity: annotation1999705
display id: annotation1999705
version: 1
top level: BBa_K106672
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K106672/annotation1999705/range1999705/1

https://synbiohub.org/public/igem/BBa_K106672/annotation1999705/range1999705/1
type: Range
persistent identity: range1999705
display id: range1999705
version: 1
direction: direction/forward
top level: BBa_K106672
start: 224
end: 242
orientation: inline

Stop codons in all 3 frames
type: SequenceAnnotation
persistent identity: annotation1999713
display id: annotation1999713
version: 1
top level: BBa_K106672
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K106672/annotation1999713/range1999713/1

https://synbiohub.org/public/igem/BBa_K106672/annotation1999713/range1999713/1
type: Range
persistent identity: range1999713
display id: range1999713
version: 1
direction: direction/forward
top level: BBa_K106672
start: 11
end: 21
orientation: inline

T3 promoter
type: SequenceAnnotation
persistent identity: annotation1999711
display id: annotation1999711
version: 1
top level: BBa_K106672
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K106672/annotation1999711/range1999711/1

https://synbiohub.org/public/igem/BBa_K106672/annotation1999711/range1999711/1
type: Range
persistent identity: range1999711
display id: range1999711
version: 1
direction: direction/forward
top level: BBa_K106672
start: 5581
end: 5600
orientation: inline

A overhang
type: SequenceAnnotation
persistent identity: annotation1999715
display id: annotation1999715
version: 1
top level: BBa_K106672
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K106672/annotation1999715/range1999715/1

https://synbiohub.org/public/igem/BBa_K106672/annotation1999715/range1999715/1
type: Range
persistent identity: range1999715
display id: range1999715
version: 1
direction: direction/forward
top level: BBa_K106672
start: 6635
end: 6638
orientation: inline

ampicillin resistance promoter
type: SequenceAnnotation
persistent identity: annotation1999708
display id: annotation1999708
version: 1
top level: BBa_K106672
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K106672/annotation1999708/range1999708/1

https://synbiohub.org/public/igem/BBa_K106672/annotation1999708/range1999708/1
type: Range
persistent identity: range1999708
display id: range1999708
version: 1
direction: direction/forward
top level: BBa_K106672
start: 3469
end: 3497
orientation: inline

D overhang
type: SequenceAnnotation
persistent identity: annotation1993689
display id: annotation1993689
version: 1
top level: BBa_K106672
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K106672/annotation1993689/range1993689/1

https://synbiohub.org/public/igem/BBa_K106672/annotation1993689/range1993689/1
type: Range
persistent identity: range1993689
display id: range1993689
version: 1
direction: direction/forward
top level: BBa_K106672
start: 1
end: 4
orientation: inline

Gal1 Promoter
type: SequenceAnnotation
persistent identity: annotation1993687
display id: annotation1993687
version: 1
top level: BBa_K106672
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K106672/annotation1993687/range1993687/1

https://synbiohub.org/public/igem/BBa_K106672/annotation1993687/range1993687/1
type: Range
persistent identity: range1993687
display id: range1993687
version: 1
direction: direction/forward
top level: BBa_K106672
start: 5618
end: 6634
orientation: inline