Variant of pBca1021 (J61003) with GFP inserted. In practice, it is a E0040 basic part just in a different pSB1A* plasmid.
Verification forward (VF2) primer binding site
beta-lactamase (ampicillin resistance marker)
Verification reverse (VR) primer binding site
Variant of pBca1021 (J61003) with GFP inserted. Part J23019 is the same as J23018 but in pSB3C6.
chloramphenicol acetyl transferase (CmR resistance marker)
Plasmid for assembly of open reading frame basic parts. A Tet promoter (r0040) and strong ribosome binding site (b0034) lie between the EcoRI and XbaI sites. Insertion of an open reading frame basic part into the XbaI and PstI sites results in constitutive expression but retains basic part utilityin standard assembly.
PCR KB001/ca1021R on pSB1A2-J01022 (250 bp, EcoRI/XbaI) sub into pSB1A2-E0040(GFP;DNA-1-5H) (EcoRI/XbaI) Product is pBca1021-E0040 ---- ca1021R Reverse b0034 XbaI AGCCATCTAGAATTTCTCCTCTTTCTCTAG KB001 Forward EcoRI to construct Ptet part-making biobrick plasmid ttctggaattcgcggccgcaactagagccaggcatc