//function/sensor/light
type: Collection
persistent identity: function sensor light
display id: function_sensor_light
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
owned by: james
owned by: myers
member: BBa_K106010
member: BBa_K1660004
member: BBa_K990004
member: YtvA
member: BBa_K191008
member: BBa_K881001
member: BBa_S05054
member: BBa_K559000
member: BBa_K191002
member: BBa_K772102
member: BBa_K881002
member: Lov-tetR
member: BBa_K786002
member: BBa_K191003
member: BBa_M36921
member: cph8
member: BBa_K785004
member: BBa_I15008
member: BBa_K106009
member: BBa_K1660006
member: BBa_K786003
member: BBa_K1587008
member: BBa_K081017
member: LovTAP
member: BBa_K1936001
member: BBa_K1982003
member: BBa_K360121
member: BBa_K1982009
member: BBa_I15009
member: BBa_I15010
member: BBa_K1982005
member: BBa_K786001
member: BBa_K1660007
member: BBa_K559010
member: BBa_K191009
member: BBa_K786004
member: BBa_K881000
member: BBa_S05053
member: BBa_K1982002
member: BBa_K1982010
member: BBa_K1732028
member: BBa_K559001
member: CP919

Lov-tetR
type: ComponentDefinition
persistent identity: BBa K785000
display id: BBa_K785000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Lov-tetR (light sensor with Ptet repression) light->PtetO repressed
creator: Zining Hou
created: 2012-09-20T11:00:00Z
modified: 2015-05-08T01:13:22Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1040_
m user id: 0
owner id: 11000
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This part code a protein as a light sensor and repressor. With the existence of this protein, expression downstream the PtetO promoter will be repressed under the 450nm blue light. This protein, which is actually a well designed fusion protein, include two domain. The Lov domain is from Avena sativa phototrophin1(AsLOV2). The tetR is from the tetracycline resistance, which could combine the tetracycline and enable the expression of the tetA. We carefully designed the linker between these two parts, and fulfilled our sensor part in our biowave circuit.
mutable notes: This part is designed for our biowave project.
mutable provenance: This coding sequence is form the Avena sativa phototrophin1(AsLOV2) and tetracycline resistance. The whole gene is synthesized by Invitrogen company.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: linker part
sequence annotation: tetR DNA binding domain
sequence annotation: Lov light sensor domain
sequence: https://synbiohub.org/public/igem/BBa_K785000_sequence/1

linker part
type: SequenceAnnotation
persistent identity: annotation2194042
display id: annotation2194042
version: 1
top level: Lov-tetR
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K785000/annotation2194042/range2194042/1

https://synbiohub.org/public/igem/BBa_K785000/annotation2194042/range2194042/1
type: Range
persistent identity: range2194042
display id: range2194042
version: 1
direction: direction/forward
top level: Lov-tetR
start: 213
end: 345
orientation: inline

tetR DNA binding domain
type: SequenceAnnotation
persistent identity: annotation2194043
display id: annotation2194043
version: 1
top level: Lov-tetR
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K785000/annotation2194043/range2194043/1

https://synbiohub.org/public/igem/BBa_K785000/annotation2194043/range2194043/1
type: Range
persistent identity: range2194043
display id: range2194043
version: 1
direction: direction/forward
top level: Lov-tetR
start: 294
end: 1044
orientation: inline

Lov light sensor domain
type: SequenceAnnotation
persistent identity: annotation2194041
display id: annotation2194041
version: 1
top level: Lov-tetR
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K785000/annotation2194041/range2194041/1

https://synbiohub.org/public/igem/BBa_K785000/annotation2194041/range2194041/1
type: Range
persistent identity: range2194041
display id: range2194041
version: 1
direction: direction/forward
top level: Lov-tetR
start: 1
end: 294
orientation: inline

LovTAP
type: ComponentDefinition
persistent identity: BBa K191006
display id: BBa_K191006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LOVTAP
creator: Le Thanh Tu NGUYEN
created: 2009-10-15T11:00:00Z
modified: 2015-05-08T01:11:15Z
discontinued: false
dominant: false
experience: experience/None
group u list: _287_
m user id: 0
owner id: 4198
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: Coding sequence of a fused protein (LOV means light, oxygen, and voltage,represent a light sensitive protein domain whereas TAP means tryptophan-activated protein).
mutable notes: LOVTAP sequence contains 2 Pst1 restriction sites
mutable provenance: Kindly provided by the authors of : Light-activated DNA binding in a designed allosteric protein; Strickland et al., Proceedings of the National Academy of Sciences (2008) vol. 105 (31) pp. 10709
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K191006_sequence/1

BBa_K559010
type: ComponentDefinition
persistent identity: BBa K559010
display id: BBa_K559010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Halorhodopsin complete system
creator: Jacky, Fong Chuen, Loo
created: 2011-09-28T11:00:00Z
modified: 2015-05-08T01:12:41Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _727_
m user id: 0
owner id: 6123
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: Halorhodopsin gene allow chloride ion to be pumped in by the level of light intensity. Halorhodopsin gene is under regulation by constitute T7 promoter to allow protein coding to be maximized, and the bidirectional terminator is added for high efficiency termination of both forward and backward transcription
mutable notes: The double terminator wass tested to stop effectively on the transcription of both forward and backward transcription to make our protein generator be a controlled system
mutable provenance: Halorhodopsin comes from part BBa_K559000, which is come from genomic sequence of N.pharaonis (genebank ID:J05199.1)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K559001
component: BBa_B0014
sequence annotation: BBa_K559001
sequence annotation: BBa_B0014
sequence: https://synbiohub.org/public/igem/BBa_K559010_sequence/1

BBa_K559001
type: Component
persistent identity: component2177574
display id: component2177574
version: 1
top level: BBa_K559010
access: public
definition: BBa_K559001

BBa_B0014
type: Component
persistent identity: component2177581
display id: component2177581
version: 1
top level: BBa_K559010
access: public
definition: https://synbiohub.org/public/igem/BBa_B0014/1

BBa_K559001
type: SequenceAnnotation
persistent identity: annotation2177574
display id: annotation2177574
version: 1
top level: BBa_K559010
location: https://synbiohub.org/public/igem/BBa_K559010/annotation2177574/range2177574/1
component: BBa_K559001

https://synbiohub.org/public/igem/BBa_K559010/annotation2177574/range2177574/1
type: Range
persistent identity: range2177574
display id: range2177574
version: 1
direction: direction/reverse
top level: BBa_K559010
start: 1
end: 728
orientation: inline

BBa_B0014
type: SequenceAnnotation
persistent identity: annotation2177581
display id: annotation2177581
version: 1
top level: BBa_K559010
location: https://synbiohub.org/public/igem/BBa_K559010/annotation2177581/range2177581/1
component: BBa_B0014

https://synbiohub.org/public/igem/BBa_K559010/annotation2177581/range2177581/1
type: Range
persistent identity: range2177581
display id: range2177581
version: 1
direction: direction/reverse
top level: BBa_K559010
start: 737
end: 831
orientation: inline

CP919
type: ComponentDefinition
persistent identity: BBa V1012
display id: BBa_V1012
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: E. coli strain CP919
creator: Meagan Lizarazo
created: 2006-07-18T11:00:00Z
modified: 2015-05-08T01:14:53Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 457
owning group id: 67
sample status: No part sequence
status: status/Unavailable
bookmark: false
mutable description: Cell for the light sensing parts
mutable notes: None
mutable provenance: From UT Austin
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Cell
role: SO:0000340

cph8
type: ComponentDefinition
persistent identity: BBa K592000
display id: BBa_K592000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: cph8 (Cph1/EnvZ fusion)
creator: Lei Sun
created: 2011-09-17T11:00:00Z
modified: 2015-05-08T01:12:48Z
discontinued: false
dominant: false
experience: experience/None
group u list: _763_
m user id: 0
owner id: 7929
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part is identical to BBa_I15010, the cph8 red light sensor. We were unable to transform BBa_I15010 from the the 2011 iGEM distribution kit. PCR attempts using VF2 and VR failed to amplify any DNA from the well sample. In the end, we chose to amplify cph8 from Jeffrey Tabor's pJT122 plasmid provided by Chris Voigt. The cph8 in pJT122 contained an internal PstI site, which was removed by point-mutagenesis. The resultant DNA sequence is identical to that of BBa_I15010.
mutable notes: The source DNA (pJT122) contained illegal restriction site (PstI) that had to be removed.
mutable provenance: Jeffrey Tabor's pJT122 plasmid
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: cph8
sequence: https://synbiohub.org/public/igem/BBa_K592000_sequence/1

cph8
type: SequenceAnnotation
persistent identity: annotation2132074
display id: annotation2132074
version: 1
top level: cph8
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K592000/annotation2132074/range2132074/1

https://synbiohub.org/public/igem/BBa_K592000/annotation2132074/range2132074/1
type: Range
persistent identity: range2132074
display id: range2132074
version: 1
direction: direction/forward
top level: cph8
start: 1
end: 2238
orientation: inline

BBa_K881001
type: ComponentDefinition
persistent identity: BBa K881001
display id: BBa_K881001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: G-GECO1.1
creator: Nick van der Veeken
created: 2012-09-19T11:00:00Z
modified: 2015-05-08T01:13:40Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1144_
m user id: 0
owner id: 11729
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: Engineered green fluorescent protein that modulates its fluorescence in response to changes in calcium ion (Ca2+) concentration.
mutable notes: -
mutable provenance: Zhao et al. 2011, Science 30 September 2011: Vol. 333 no. 6051 pp. 1888-1891, DOI: 10.1126/science.1208592
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K881001_sequence/1

BBa_K1982002
type: ComponentDefinition
persistent identity: BBa K1982002
display id: BBa_K1982002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Prokaryotic Cryptochrome 2 (CRY2) ( a blue light stimulated photoreceptor)
creator: Zexu Li
created: 2016-09-10T11:00:00Z
modified: 2016-10-09T12:15:31Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2449_
m user id: 30176
owner id: 30176
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Cryptochrome 2 (CRY2) is a blue light stimulated photoreceptor, when exposed to blue light, it would interact withthe N-terminal fragment of CIB1 (CIBN) . This part is full-length CRY2 CDS. Optogenetic systems enable precise spatial and temporal control of cell behavior. A light-activated CRISPR/Cas9 effector (LACE) system that induces transcription of endogenous genes in the presence of blue light.This was accomplished by fusing the light-inducible heterodimerizing proteins CRY2 and CIB1 to a transactivation domain and the catalytically inactive tCas9, respectively. The versatile LACE system can be easily directed to new DNA sequences for the dynamic regulation of endogenous genes.
mutable notes: mutant EcoR 1 : TGTTGGGAATTc--->TGTTGGGAATTg
mutable provenance: The light-inducible heterodimerizering proteins CRY2 and CIB1 are both from Arabidopsis thaliana.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: ATG
sequence annotation: Prokaryotic rbs
sequence: https://synbiohub.org/public/igem/BBa_K1982002_sequence/1

ATG
type: SequenceAnnotation
persistent identity: annotation2483021
display id: annotation2483021
version: 1
top level: BBa_K1982002
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1982002/annotation2483021/range2483021/1

https://synbiohub.org/public/igem/BBa_K1982002/annotation2483021/range2483021/1
type: Range
persistent identity: range2483021
display id: range2483021
version: 1
direction: direction/forward
top level: BBa_K1982002
start: 19
end: 21
orientation: inline

Prokaryotic rbs
type: SequenceAnnotation
persistent identity: annotation2488670
display id: annotation2488670
version: 1
top level: BBa_K1982002
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1982002/annotation2488670/range2488670/1

https://synbiohub.org/public/igem/BBa_K1982002/annotation2488670/range2488670/1
type: Range
persistent identity: range2488670
display id: range2488670
version: 1
direction: direction/forward
top level: BBa_K1982002
start: 1
end: 18
orientation: inline

BBa_K191003
type: ComponentDefinition
persistent identity: BBa K191003
display id: BBa_K191003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Lac I - RBS - LovTAP - Term
creator: Le Thanh Tu NGUYEN
created: 2009-10-10T11:00:00Z
modified: 2015-05-08T01:11:15Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _287_
m user id: 0
owner id: 4198
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: Complete coding sequence for fused protein LOVTAP, with inducible promoter, RBS site and terminator.
mutable notes: 2 restriction sites of Pst1 in LOVTAP
mutable provenance: See subparts' references
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K191006
component: BBa_B0012
component: BBa_K191001
component: BBa_B0010
sequence annotation: BBa_K191001
sequence annotation: BBa_K191006
sequence annotation: BBa_B0012
sequence annotation: BBa_B0010
sequence: https://synbiohub.org/public/igem/BBa_K191003_sequence/1

BBa_K191006
type: Component
persistent identity: component2222427
display id: component2222427
version: 1
top level: BBa_K191003
access: public
definition: LovTAP

BBa_B0012
type: Component
persistent identity: component2222430
display id: component2222430
version: 1
top level: BBa_K191003
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_K191001
type: Component
persistent identity: component2222426
display id: component2222426
version: 1
top level: BBa_K191003
access: public
definition: https://synbiohub.org/public/igem/BBa_K191001/1

BBa_B0010
type: Component
persistent identity: component2222428
display id: component2222428
version: 1
top level: BBa_K191003
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_K191001
type: SequenceAnnotation
persistent identity: annotation2222426
display id: annotation2222426
version: 1
top level: BBa_K191003
location: https://synbiohub.org/public/igem/BBa_K191003/annotation2222426/range2222426/1
component: BBa_K191001

https://synbiohub.org/public/igem/BBa_K191003/annotation2222426/range2222426/1
type: Range
persistent identity: range2222426
display id: range2222426
version: 1
direction: direction/reverse
top level: BBa_K191003
start: 1
end: 223
orientation: inline

BBa_K191006
type: SequenceAnnotation
persistent identity: annotation2222427
display id: annotation2222427
version: 1
top level: BBa_K191003
location: https://synbiohub.org/public/igem/BBa_K191003/annotation2222427/range2222427/1
component: BBa_K191006

https://synbiohub.org/public/igem/BBa_K191003/annotation2222427/range2222427/1
type: Range
persistent identity: range2222427
display id: range2222427
version: 1
direction: direction/reverse
top level: BBa_K191003
start: 230
end: 916
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2222430
display id: annotation2222430
version: 1
top level: BBa_K191003
location: https://synbiohub.org/public/igem/BBa_K191003/annotation2222430/range2222430/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K191003/annotation2222430/range2222430/1
type: Range
persistent identity: range2222430
display id: range2222430
version: 1
direction: direction/forward
top level: BBa_K191003
start: 1013
end: 1053
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2222428
display id: annotation2222428
version: 1
top level: BBa_K191003
location: https://synbiohub.org/public/igem/BBa_K191003/annotation2222428/range2222428/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K191003/annotation2222428/range2222428/1
type: Range
persistent identity: range2222428
display id: range2222428
version: 1
direction: direction/forward
top level: BBa_K191003
start: 925
end: 1004
orientation: inline

BBa_K559000
type: ComponentDefinition
persistent identity: BBa K559000
display id: BBa_K559000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Halorhodopsin
creator: Jacky, Fong Chuen, Loo
created: 2011-09-28T11:00:00Z
modified: 2015-05-08T01:12:41Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _727_
m user id: 0
owner id: 6123
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This Halorhodopsin gene allow the photoreceptor protein be expressed, to regulate the chloride ion pumped in by light intensity
mutable notes: The DNA sequence selected from ID J05199.1 of 61nt to 700nt for protein coding
mutable provenance: It is from N.pharaonis, gene back ID J05199.1
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Halorhodopsin
sequence: https://synbiohub.org/public/igem/BBa_K559000_sequence/1

Halorhodopsin
type: SequenceAnnotation
persistent identity: annotation2147609
display id: annotation2147609
version: 1
top level: BBa_K559000
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K559000/annotation2147609/range2147609/1

https://synbiohub.org/public/igem/BBa_K559000/annotation2147609/range2147609/1
type: Range
persistent identity: range2147609
display id: range2147609
version: 1
direction: direction/forward
top level: BBa_K559000
start: 1
end: 640
orientation: inline

BBa_K785004
type: ComponentDefinition
persistent identity: BBa K785004
display id: BBa_K785004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Lov-HTH (light sensor with Ptet repression) light->PtetO repressed
creator: Zining Hou
created: 2012-09-22T11:00:00Z
modified: 2015-05-08T01:13:22Z
discontinued: false
dominant: false
group u list: _1040_
m user id: 0
owner id: 11000
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This page is about a light sensor and regulator protein: Lov-HTH. Lov-HTH is a artificial light sensor designed by iGEM12 Fudan Lux team. This protein include two function domain, Lov the light sensing domain and HTH the DNA binding domain.
mutable notes: The HTH motif swap is the main issue in our design. We carefully choose the HTH sequence and swap it into the original amino acid sequence.
mutable provenance: The sequence is reverse translated from the protein EL222 http://www.rcsb.org/pdb/explore/explore.do?structureId=3P7N which is from Erythrobacter litoralis. The DNA is synthesized by Invitrogen company.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: HTH DNA binding domain
sequence annotation: Lov light sensing domain
sequence: https://synbiohub.org/public/igem/BBa_K785004_sequence/1

HTH DNA binding domain
type: SequenceAnnotation
persistent identity: annotation2194603
display id: annotation2194603
version: 1
top level: BBa_K785004
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K785004/annotation2194603/range2194603/1

https://synbiohub.org/public/igem/BBa_K785004/annotation2194603/range2194603/1
type: Range
persistent identity: range2194603
display id: range2194603
version: 1
direction: direction/forward
top level: BBa_K785004
start: 292
end: 672
orientation: inline

Lov light sensing domain
type: SequenceAnnotation
persistent identity: annotation2194602
display id: annotation2194602
version: 1
top level: BBa_K785004
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K785004/annotation2194602/range2194602/1

https://synbiohub.org/public/igem/BBa_K785004/annotation2194602/range2194602/1
type: Range
persistent identity: range2194602
display id: range2194602
version: 1
direction: direction/forward
top level: BBa_K785004
start: 1
end: 291
orientation: inline

BBa_K106009
type: ComponentDefinition
persistent identity: BBa K106009
display id: BBa_K106009
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Pif3, Aar1 AB part
creator: Willis Wong
created: 2008-10-22T11:00:00Z
modified: 2015-05-08T01:08:58Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _226_
m user id: 0
owner id: 3412
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This is a coding sequence for Pif3 gene with BD ends for Aar1 cloning. Binds with PhyB at 720nm light and unbinds at 660nm.
mutable notes: As a BD part, there is no ATG, and the stop codon is included. For more information on AarI cloning, please refer to the UCSF 2008 igem page.
mutable provenance: Arabidopsis thaliana
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K106009_sequence/1

BBa_K1982003
type: ComponentDefinition
persistent identity: BBa K1982003
display id: BBa_K1982003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CIBN(the N-terminal fragment of CIB1)
creator: Zexu Li
created: 2016-09-11T11:00:00Z
modified: 2016-09-12T05:35:21Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2449_
m user id: 30176
owner id: 30176
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Encodes the N-terminal fragment of transcription factor CIB1 (cryptochrome-interacting basic-helix-loop-helix). CIB1 interacts with CRY2 (cryptochrome 2) in a blue light-specific manner in yeast and Arabidopsis cells, and it acts together with additional CIB1-related proteins to promote CRY2-dependent floral initiation. CIB1 positively regulates FT expression. Cryptochrome 2 (CRY2) is a blue light stimulated photoreceptor, when exposed to blue light, it would interact withthe N-terminal fragment of CIB1 (CIBN) . Optogenetic systems enable precise spatial and temporal control of cell behavior. A light-activated CRISPR/Cas9 effector (LACE) system that induces transcription of endogenous genes in the presence of blue light.This was accomplished by fusing the light-inducible heterodimerizing proteins CRY2 and CIB1 to a transactivation domain and the catalytically inactive tCas9, respectively. The versatile LACE system can be easily directed to new DNA sequences for the dynamic regulation of endogenous genes[1].
mutable notes: no
mutable provenance: The light-inducible heterodimerizering proteins CRY2 and CIB1 are both from Arabidopsis thaliana.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: TAA
sequence annotation: ATG
sequence: https://synbiohub.org/public/igem/BBa_K1982003_sequence/1

TAA
type: SequenceAnnotation
persistent identity: annotation2482939
display id: annotation2482939
version: 1
top level: BBa_K1982003
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1982003/annotation2482939/range2482939/1

https://synbiohub.org/public/igem/BBa_K1982003/annotation2482939/range2482939/1
type: Range
persistent identity: range2482939
display id: range2482939
version: 1
direction: direction/forward
top level: BBa_K1982003
start: 607
end: 612
orientation: inline

ATG
type: SequenceAnnotation
persistent identity: annotation2482938
display id: annotation2482938
version: 1
top level: BBa_K1982003
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1982003/annotation2482938/range2482938/1

https://synbiohub.org/public/igem/BBa_K1982003/annotation2482938/range2482938/1
type: Range
persistent identity: range2482938
display id: range2482938
version: 1
direction: direction/forward
top level: BBa_K1982003
start: 1
end: 3
orientation: inline

BBa_I15008
type: ComponentDefinition
persistent identity: BBa I15008
display id: BBa_I15008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: heme oxygenase (ho1) from Synechocystis
creator: Jeff Tabor
created: 2004-09-19T11:00:00Z
modified: 2015-08-31T04:07:38Z
discontinued: false
dominant: false
experience: experience/None
group u list: _5_
m user id: 0
owner id: 88
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: One of two requisite genes required for the biosynthesis of phycocyanobilin from heme.
mutable provenance: Voigt Lab via Anselm Levskaya
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_I15008_sequence/1

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2214026
display id: annotation2214026
version: 1
top level: BBa_I15008
role: SO:0000807
role: feature/barcode
location: https://synbiohub.org/public/igem/BBa_I15008/annotation2214026/range2214026/1

https://synbiohub.org/public/igem/BBa_I15008/annotation2214026/range2214026/1
type: Range
persistent identity: range2214026
display id: range2214026
version: 1
direction: direction/forward
top level: BBa_I15008
start: 727
end: 751
orientation: inline

BBa_K081017
type: ComponentDefinition
persistent identity: BBa K081017
display id: BBa_K081017
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pcyA protein generator (TERM-)
creator: Lorenzo Pasotti, Paolo Magni
created: 2008-10-18T11:00:00Z
modified: 2015-05-08T01:08:35Z
discontinued: false
dominant: true
experience: experience/None
group u list: _227_
m user id: 0
owner id: 2583
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: pcyA converts biliverdin IXalpha (BV) to phycocyanobilin (PCB). Strong RBS (efficiency=0.6).
mutable notes: We used BioBrick Standard Assembly.
mutable provenance: RBS: pcyA:
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_I15009
component: BBa_B0030
sequence annotation: BBa_B0030
sequence annotation: BBa_I15009
sequence: https://synbiohub.org/public/igem/BBa_K081017_sequence/1

BBa_I15009
type: Component
persistent identity: component2244006
display id: component2244006
version: 1
top level: BBa_K081017
access: public
definition: BBa_I15009

BBa_B0030
type: Component
persistent identity: component2244002
display id: component2244002
version: 1
top level: BBa_K081017
access: public
definition: https://synbiohub.org/public/igem/BBa_B0030/1

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation2244002
display id: annotation2244002
version: 1
top level: BBa_K081017
location: https://synbiohub.org/public/igem/BBa_K081017/annotation2244002/range2244002/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_K081017/annotation2244002/range2244002/1
type: Range
persistent identity: range2244002
display id: range2244002
version: 1
direction: direction/forward
top level: BBa_K081017
start: 1
end: 15
orientation: inline

BBa_I15009
type: SequenceAnnotation
persistent identity: annotation2244006
display id: annotation2244006
version: 1
top level: BBa_K081017
location: https://synbiohub.org/public/igem/BBa_K081017/annotation2244006/range2244006/1
component: BBa_I15009

https://synbiohub.org/public/igem/BBa_K081017/annotation2244006/range2244006/1
type: Range
persistent identity: range2244006
display id: range2244006
version: 1
direction: direction/reverse
top level: BBa_K081017
start: 22
end: 796
orientation: inline

BBa_K786002
type: ComponentDefinition
persistent identity: BBa K786002
display id: BBa_K786002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Sensory rhodopsin II (SRII) with HtrII & Tar, sensitive to blue-green light
creator: Leung Wai Tak
created: 2012-09-20T11:00:00Z
modified: 2015-05-08T01:13:22Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1041_
m user id: 0
owner id: 14285
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: Sensory rhodopsin II fusion with HtrII & Tar, which is sensitive to blue-green light, with sensing spectra covering from 400-500 nm. We used it for sensing blur-green light to achieve phototaxis and switch for downstream gene expression.
mutable notes: Pfam (version 26.0) was used to predict the domain of fusion protein. Linker was refereed and modified from previous research study of SRII fusion with HtrII.
mutable provenance: From genomic sequence of bacterial N. pharaonis (DSM 2160) and E.coli K12.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
sequence annotation: B0015
sequence annotation: BamHI for C-terminal fusion
sequence annotation: Constitutive promoter BBa_J23100
sequence annotation: HindIII for N-terminal fusin
sequence annotation: SRII-HtrII-Tar
sequence annotation: RBS 1.0
sequence: https://synbiohub.org/public/igem/BBa_K786002_sequence/1

B0015
type: SequenceAnnotation
persistent identity: annotation2199694
display id: annotation2199694
version: 1
top level: BBa_K786002
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K786002/annotation2199694/range2199694/1

https://synbiohub.org/public/igem/BBa_K786002/annotation2199694/range2199694/1
type: Range
persistent identity: range2199694
display id: range2199694
version: 1
direction: direction/forward
top level: BBa_K786002
start: 2117
end: 2245
orientation: inline

BamHI for C-terminal fusion
type: SequenceAnnotation
persistent identity: annotation2199693
display id: annotation2199693
version: 1
top level: BBa_K786002
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K786002/annotation2199693/range2199693/1

https://synbiohub.org/public/igem/BBa_K786002/annotation2199693/range2199693/1
type: Range
persistent identity: range2199693
display id: range2199693
version: 1
direction: direction/forward
top level: BBa_K786002
start: 785
end: 790
orientation: inline

Constitutive promoter BBa_J23100
type: SequenceAnnotation
persistent identity: annotation2199689
display id: annotation2199689
version: 1
top level: BBa_K786002
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K786002/annotation2199689/range2199689/1

https://synbiohub.org/public/igem/BBa_K786002/annotation2199689/range2199689/1
type: Range
persistent identity: range2199689
display id: range2199689
version: 1
direction: direction/forward
top level: BBa_K786002
start: 1
end: 41
orientation: inline

HindIII for N-terminal fusin
type: SequenceAnnotation
persistent identity: annotation2199691
display id: annotation2199691
version: 1
top level: BBa_K786002
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K786002/annotation2199691/range2199691/1

https://synbiohub.org/public/igem/BBa_K786002/annotation2199691/range2199691/1
type: Range
persistent identity: range2199691
display id: range2199691
version: 1
direction: direction/forward
top level: BBa_K786002
start: 62
end: 67
orientation: inline

SRII-HtrII-Tar
type: SequenceAnnotation
persistent identity: annotation2199692
display id: annotation2199692
version: 1
top level: BBa_K786002
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K786002/annotation2199692/range2199692/1

https://synbiohub.org/public/igem/BBa_K786002/annotation2199692/range2199692/1
type: Range
persistent identity: range2199692
display id: range2199692
version: 1
direction: direction/forward
top level: BBa_K786002
start: 68
end: 2083
orientation: inline

RBS 1.0
type: SequenceAnnotation
persistent identity: annotation2199690
display id: annotation2199690
version: 1
top level: BBa_K786002
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K786002/annotation2199690/range2199690/1

https://synbiohub.org/public/igem/BBa_K786002/annotation2199690/range2199690/1
type: Range
persistent identity: range2199690
display id: range2199690
version: 1
direction: direction/forward
top level: BBa_K786002
start: 42
end: 61
orientation: inline

BBa_K1732028
type: ComponentDefinition
persistent identity: BBa K1732028
display id: BBa_K1732028
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Renilla-His
creator: Donna Lee
created: 2015-09-17T11:00:00Z
modified: 2016-01-21T02:26:47Z
discontinued: false
dominant: false
group u list: _2154_
m user id: 4206
owner id: 27195
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Renilla (BBa_J52008) with 6X-His tagged for easier protein purification. Sequence was codon optimized for E.coli by IDT optimization tools.
mutable notes: 6X-His tagged for easier protein purification. Sequence was codon optimized for E.coli by IDT optimization tools.
mutable provenance: BBa_J52008
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: 6X-His
sequence annotation: RLuc
sequence: https://synbiohub.org/public/igem/BBa_K1732028_sequence/1

6X-His
type: SequenceAnnotation
persistent identity: annotation2469637
display id: annotation2469637
version: 1
top level: BBa_K1732028
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1732028/annotation2469637/range2469637/1

https://synbiohub.org/public/igem/BBa_K1732028/annotation2469637/range2469637/1
type: Range
persistent identity: range2469637
display id: range2469637
version: 1
direction: direction/forward
top level: BBa_K1732028
start: 933
end: 951
orientation: inline

RLuc
type: SequenceAnnotation
persistent identity: annotation2469636
display id: annotation2469636
version: 1
top level: BBa_K1732028
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1732028/annotation2469636/range2469636/1

https://synbiohub.org/public/igem/BBa_K1732028/annotation2469636/range2469636/1
type: Range
persistent identity: range2469636
display id: range2469636
version: 1
direction: direction/forward
top level: BBa_K1732028
start: 1
end: 932
orientation: inline

BBa_K786003
type: ComponentDefinition
persistent identity: BBa K786003
display id: BBa_K786003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Sensory rhodopsin I (SRI) with HtrII & Tar, sensitive to orange light
creator: Leung Wai Tak
created: 2012-09-20T11:00:00Z
modified: 2015-05-08T01:13:22Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1041_
m user id: 0
owner id: 14285
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: Sensory rhodopsin I fusion with HtrII & Tar, which is sensitive to orange light, with sensing spectra covering from 590-650 nm. We used it for sensing blur-green light to achieve phototaxis and switch for downstream gene expression.
mutable notes: Pfam (version 26.0) was used to predict the domain of fusion protein. Linker was refereed and modified from previous research study of SRI fusion with HtrII.
mutable provenance: From genomic sequence of bacterial N. pharaonis (DSM 2160) and E.coli K12.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
sequence annotation: B0015
sequence annotation: Constitutive promoter BBa_J23100
sequence annotation: SRI-HtrI-Tar
sequence annotation: RBS 1.0
sequence: https://synbiohub.org/public/igem/BBa_K786003_sequence/1

B0015
type: SequenceAnnotation
persistent identity: annotation2199719
display id: annotation2199719
version: 1
top level: BBa_K786003
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K786003/annotation2199719/range2199719/1

https://synbiohub.org/public/igem/BBa_K786003/annotation2199719/range2199719/1
type: Range
persistent identity: range2199719
display id: range2199719
version: 1
direction: direction/forward
top level: BBa_K786003
start: 2029
end: 2158
orientation: inline

Constitutive promoter BBa_J23100
type: SequenceAnnotation
persistent identity: annotation2190185
display id: annotation2190185
version: 1
top level: BBa_K786003
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K786003/annotation2190185/range2190185/1

https://synbiohub.org/public/igem/BBa_K786003/annotation2190185/range2190185/1
type: Range
persistent identity: range2190185
display id: range2190185
version: 1
direction: direction/forward
top level: BBa_K786003
start: 1
end: 41
orientation: inline

SRI-HtrI-Tar
type: SequenceAnnotation
persistent identity: annotation2199706
display id: annotation2199706
version: 1
top level: BBa_K786003
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K786003/annotation2199706/range2199706/1

https://synbiohub.org/public/igem/BBa_K786003/annotation2199706/range2199706/1
type: Range
persistent identity: range2199706
display id: range2199706
version: 1
direction: direction/forward
top level: BBa_K786003
start: 68
end: 1992
orientation: inline

RBS 1.0
type: SequenceAnnotation
persistent identity: annotation2190186
display id: annotation2190186
version: 1
top level: BBa_K786003
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K786003/annotation2190186/range2190186/1

https://synbiohub.org/public/igem/BBa_K786003/annotation2190186/range2190186/1
type: Range
persistent identity: range2190186
display id: range2190186
version: 1
direction: direction/forward
top level: BBa_K786003
start: 42
end: 61
orientation: inline

BBa_K1982010
type: ComponentDefinition
persistent identity: BBa K1982010
display id: BBa_K1982010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CRY2-VP64(Eukaryotic LACE system)
creator: Zexu Li
created: 2016-10-08T11:00:00Z
modified: 2016-10-18T10:53:31Z
discontinued: false
dominant: false
group u list: _2449_
m user id: 30176
owner id: 30176
owning group id: 9
status: status/Planning
bookmark: false
mutable description: {| style="color:black" cellpadding="6" cellspacing="1" border="2" align="right" ! colspan="2" style="background:#FFBF00;"|RBS-CRY2-VP64-HA-FLAG |- |'''Function''' |gene activation |- |'''Use in''' |Prokaryotic cells |- |'''RFC standard''' |[http://parts.igem.org/Help:Assembly_standard_10 RFC 10] |- |'''Backbone''' |pSB1C3 |- |'''Submitted by''' |[http://2016.igem.org/Team:NEU-China NEU-China 2016] |} The CRY2/CIBN interaction is entirely genetically encoded. The binding reverses within minutes in the dark, allowing rapid shutoff of transcription by placing samples in the dark. This fusion protein is for use in LACE(light-activated CRISPR/Cas9 effector) system, and a VP64 fused to its C terminus. To regulate DNA transcription by blue light, the system is based on CRY2/CIBN interaction in which a light-mediated protein interaction brings together two protein (tCas9 and an activation domain VP64) . If we remove the stimulation of blue light, dark reversion of CRY2 will dissociate the interaction with CIBN and shut off transcription. We used the full-length CRY2 (BBa_K1982002) and fused it to the transcription activator domain VP64 (BBa_K1982000). An prokaryotic RBS sequence from the Community collection(BBa_B0034) fused to the beginning of CRY2-VP64. For detection of expression the fusion protein was tagged with a FLAG-epitope coding sequence (gactacaaggacgacgacgacaaa) .Figure 1 illustrates the detailed design of the whole device. <!-- Add more about the biology of this part here ===Usage and Biology=== Protein data table for BioBrick <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_ ">BBa_ automatically created by the BioBrick-AutoAnnotator version 1.0 Nucleotide sequence in RFC 10: (underlined part encodes the protein) ATGAAGATG ... GACGACAAATAATAA ORF from nucleotide position 1 to 2076 (excluding stop-codon) Amino acid sequence: (RFC 25 scars in shown in bold, other sequence features underlined; both given below) 1 101 201 301 401 501 601 MKMDKKTIVWFRRDL…
mutable notes: no
mutable provenance: CRY2 and CIB1 are extracted from Arabidopsis thaliana. tCas9 is from GE Share company. VP64 is synthetic construct.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: VP16
sequence annotation: CRY2
sequence annotation: ATG
sequence annotation: Eukaryotic rbs
sequence annotation: Linker
sequence annotation: TAA
sequence annotation: FLAG
sequence annotation: HA
sequence annotation: SV40 NLS
sequence: https://synbiohub.org/public/igem/BBa_K1982010_sequence/1

VP16
type: SequenceAnnotation
persistent identity: annotation2517286
display id: annotation2517286
version: 1
top level: BBa_K1982010
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1982010/annotation2517286/range2517286/1

https://synbiohub.org/public/igem/BBa_K1982010/annotation2517286/range2517286/1
type: Range
persistent identity: range2517286
display id: range2517286
version: 1
direction: direction/forward
top level: BBa_K1982010
start: 1954
end: 1992
orientation: inline

VP16
type: SequenceAnnotation
persistent identity: annotation2517284
display id: annotation2517284
version: 1
top level: BBa_K1982010
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1982010/annotation2517284/range2517284/1

https://synbiohub.org/public/igem/BBa_K1982010/annotation2517284/range2517284/1
type: Range
persistent identity: range2517284
display id: range2517284
version: 1
direction: direction/forward
top level: BBa_K1982010
start: 1876
end: 1914
orientation: inline

CRY2
type: SequenceAnnotation
persistent identity: annotation2517282
display id: annotation2517282
version: 1
top level: BBa_K1982010
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1982010/annotation2517282/range2517282/1

https://synbiohub.org/public/igem/BBa_K1982010/annotation2517282/range2517282/1
type: Range
persistent identity: range2517282
display id: range2517282
version: 1
direction: direction/forward
top level: BBa_K1982010
start: 22
end: 1854
orientation: inline

VP16
type: SequenceAnnotation
persistent identity: annotation2517285
display id: annotation2517285
version: 1
top level: BBa_K1982010
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1982010/annotation2517285/range2517285/1

https://synbiohub.org/public/igem/BBa_K1982010/annotation2517285/range2517285/1
type: Range
persistent identity: range2517285
display id: range2517285
version: 1
direction: direction/forward
top level: BBa_K1982010
start: 1915
end: 1953
orientation: inline

ATG
type: SequenceAnnotation
persistent identity: annotation2517281
display id: annotation2517281
version: 1
top level: BBa_K1982010
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1982010/annotation2517281/range2517281/1

https://synbiohub.org/public/igem/BBa_K1982010/annotation2517281/range2517281/1
type: Range
persistent identity: range2517281
display id: range2517281
version: 1
direction: direction/forward
top level: BBa_K1982010
start: 19
end: 21
orientation: inline

Eukaryotic rbs
type: SequenceAnnotation
persistent identity: annotation2517280
display id: annotation2517280
version: 1
top level: BBa_K1982010
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1982010/annotation2517280/range2517280/1

https://synbiohub.org/public/igem/BBa_K1982010/annotation2517280/range2517280/1
type: Range
persistent identity: range2517280
display id: range2517280
version: 1
direction: direction/forward
top level: BBa_K1982010
start: 1
end: 18
orientation: inline

Linker
type: SequenceAnnotation
persistent identity: annotation2517288
display id: annotation2517288
version: 1
top level: BBa_K1982010
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1982010/annotation2517288/range2517288/1

https://synbiohub.org/public/igem/BBa_K1982010/annotation2517288/range2517288/1
type: Range
persistent identity: range2517288
display id: range2517288
version: 1
direction: direction/forward
top level: BBa_K1982010
start: 2032
end: 2037
orientation: inline

VP16
type: SequenceAnnotation
persistent identity: annotation2517287
display id: annotation2517287
version: 1
top level: BBa_K1982010
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1982010/annotation2517287/range2517287/1

https://synbiohub.org/public/igem/BBa_K1982010/annotation2517287/range2517287/1
type: Range
persistent identity: range2517287
display id: range2517287
version: 1
direction: direction/forward
top level: BBa_K1982010
start: 1993
end: 2031
orientation: inline

TAA
type: SequenceAnnotation
persistent identity: annotation2517291
display id: annotation2517291
version: 1
top level: BBa_K1982010
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1982010/annotation2517291/range2517291/1

https://synbiohub.org/public/igem/BBa_K1982010/annotation2517291/range2517291/1
type: Range
persistent identity: range2517291
display id: range2517291
version: 1
direction: direction/forward
top level: BBa_K1982010
start: 2095
end: 2100
orientation: inline

FLAG
type: SequenceAnnotation
persistent identity: annotation2517290
display id: annotation2517290
version: 1
top level: BBa_K1982010
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1982010/annotation2517290/range2517290/1

https://synbiohub.org/public/igem/BBa_K1982010/annotation2517290/range2517290/1
type: Range
persistent identity: range2517290
display id: range2517290
version: 1
direction: direction/forward
top level: BBa_K1982010
start: 2071
end: 2094
orientation: inline

HA
type: SequenceAnnotation
persistent identity: annotation2517289
display id: annotation2517289
version: 1
top level: BBa_K1982010
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1982010/annotation2517289/range2517289/1

https://synbiohub.org/public/igem/BBa_K1982010/annotation2517289/range2517289/1
type: Range
persistent identity: range2517289
display id: range2517289
version: 1
direction: direction/forward
top level: BBa_K1982010
start: 2037
end: 2063
orientation: inline

SV40 NLS
type: SequenceAnnotation
persistent identity: annotation2517283
display id: annotation2517283
version: 1
top level: BBa_K1982010
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1982010/annotation2517283/range2517283/1

https://synbiohub.org/public/igem/BBa_K1982010/annotation2517283/range2517283/1
type: Range
persistent identity: range2517283
display id: range2517283
version: 1
direction: direction/forward
top level: BBa_K1982010
start: 1855
end: 1875
orientation: inline

BBa_K881002
type: ComponentDefinition
persistent identity: BBa K881002
display id: BBa_K881002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: B-GECO1
creator: Nick van der Veeken
created: 2012-09-19T11:00:00Z
modified: 2015-05-08T01:13:40Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1144_
m user id: 0
owner id: 11729
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: Engineered blue fluorescent protein that modulates its fluorescence in response to changes in calcium ion (Ca2+) concentration.
mutable notes: -
mutable provenance: Zhao et al. 2011, Science 30 September 2011: Vol. 333 no. 6051 pp. 1888-1891, DOI: 10.1126/science.1208592
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K881002_sequence/1

BBa_K1660004
type: ComponentDefinition
persistent identity: BBa K1660004
display id: BBa_K1660004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PcyA+Ho1
creator: Dai Yuanyi
created: 2015-09-06T11:00:00Z
modified: 2015-09-07T11:31:12Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2078_
m user id: 26933
owner id: 26933
owning group id: 9
status: status/Planning
bookmark: true
mutable description: In order to work,Cph8 has to form chromophore with PCB biosynthetic genes, where the formation of PCB requires the gene pcyA and hol to function as accounted below. Hol is a sort of Iron red pigment oxidase which can oxidize the heme group using a ferredoxin cofactor, generating biliverdin IXalpha. And then, PcyA, a kind of ferredoxin oxidase from Synechocystis Sauv, functions to turn biliverdin IXalpha (BV) into PCB. We connected gene pcyA（BBa_I15009）and ho1（BBa_I15008）together along with the constitutive promoter(BBa_J23100) to ensure a continuous work of the photoinduction system in our E.coli.
mutable notes: We used oe-pcr to assemble these parts together seamlessly.
mutable provenance: pcyA gene and ho1 gene both come from Synechocystis.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: start codon
sequence annotation: B0034
sequence annotation: stop codon
sequence annotation: ho1 barcode
sequence annotation: pcyA barcode
sequence annotation: pcyA
sequence annotation: J23100
sequence annotation: ho1
sequence: https://synbiohub.org/public/igem/BBa_K1660004_sequence/1

start codon
type: SequenceAnnotation
persistent identity: annotation2445938
display id: annotation2445938
version: 1
top level: BBa_K1660004
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1660004/annotation2445938/range2445938/1

https://synbiohub.org/public/igem/BBa_K1660004/annotation2445938/range2445938/1
type: Range
persistent identity: range2445938
display id: range2445938
version: 1
direction: direction/forward
top level: BBa_K1660004
start: 835
end: 837
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation2445884
display id: annotation2445884
version: 1
top level: BBa_K1660004
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1660004/annotation2445884/range2445884/1

https://synbiohub.org/public/igem/BBa_K1660004/annotation2445884/range2445884/1
type: Range
persistent identity: range2445884
display id: range2445884
version: 1
direction: direction/forward
top level: BBa_K1660004
start: 823
end: 834
orientation: inline

stop codon
type: SequenceAnnotation
persistent identity: annotation2445868
display id: annotation2445868
version: 1
top level: BBa_K1660004
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1660004/annotation2445868/range2445868/1

https://synbiohub.org/public/igem/BBa_K1660004/annotation2445868/range2445868/1
type: Range
persistent identity: range2445868
display id: range2445868
version: 1
direction: direction/forward
top level: BBa_K1660004
start: 792
end: 797
orientation: inline

ho1 barcode
type: SequenceAnnotation
persistent identity: annotation2445942
display id: annotation2445942
version: 1
top level: BBa_K1660004
role: SO:0000807
role: feature/barcode
location: https://synbiohub.org/public/igem/BBa_K1660004/annotation2445942/range2445942/1

https://synbiohub.org/public/igem/BBa_K1660004/annotation2445942/range2445942/1
type: Range
persistent identity: range2445942
display id: range2445942
version: 1
direction: direction/forward
top level: BBa_K1660004
start: 1561
end: 1585
orientation: inline

stop codon
type: SequenceAnnotation
persistent identity: annotation2445943
display id: annotation2445943
version: 1
top level: BBa_K1660004
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1660004/annotation2445943/range2445943/1

https://synbiohub.org/public/igem/BBa_K1660004/annotation2445943/range2445943/1
type: Range
persistent identity: range2445943
display id: range2445943
version: 1
direction: direction/forward
top level: BBa_K1660004
start: 1558
end: 1560
orientation: inline

start codon
type: SequenceAnnotation
persistent identity: annotation2445865
display id: annotation2445865
version: 1
top level: BBa_K1660004
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1660004/annotation2445865/range2445865/1

https://synbiohub.org/public/igem/BBa_K1660004/annotation2445865/range2445865/1
type: Range
persistent identity: range2445865
display id: range2445865
version: 1
direction: direction/forward
top level: BBa_K1660004
start: 48
end: 50
orientation: inline

pcyA barcode
type: SequenceAnnotation
persistent identity: annotation2445869
display id: annotation2445869
version: 1
top level: BBa_K1660004
role: SO:0000807
role: feature/barcode
location: https://synbiohub.org/public/igem/BBa_K1660004/annotation2445869/range2445869/1

https://synbiohub.org/public/igem/BBa_K1660004/annotation2445869/range2445869/1
type: Range
persistent identity: range2445869
display id: range2445869
version: 1
direction: direction/forward
top level: BBa_K1660004
start: 798
end: 822
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation2445864
display id: annotation2445864
version: 1
top level: BBa_K1660004
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1660004/annotation2445864/range2445864/1

https://synbiohub.org/public/igem/BBa_K1660004/annotation2445864/range2445864/1
type: Range
persistent identity: range2445864
display id: range2445864
version: 1
direction: direction/forward
top level: BBa_K1660004
start: 36
end: 47
orientation: inline

pcyA
type: SequenceAnnotation
persistent identity: annotation2445867
display id: annotation2445867
version: 1
top level: BBa_K1660004
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1660004/annotation2445867/range2445867/1

https://synbiohub.org/public/igem/BBa_K1660004/annotation2445867/range2445867/1
type: Range
persistent identity: range2445867
display id: range2445867
version: 1
direction: direction/forward
top level: BBa_K1660004
start: 48
end: 750
orientation: inline

J23100
type: SequenceAnnotation
persistent identity: annotation2445845
display id: annotation2445845
version: 1
top level: BBa_K1660004
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1660004/annotation2445845/range2445845/1

https://synbiohub.org/public/igem/BBa_K1660004/annotation2445845/range2445845/1
type: Range
persistent identity: range2445845
display id: range2445845
version: 1
direction: direction/forward
top level: BBa_K1660004
start: 1
end: 35
orientation: inline

ho1
type: SequenceAnnotation
persistent identity: annotation2445885
display id: annotation2445885
version: 1
top level: BBa_K1660004
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1660004/annotation2445885/range2445885/1

https://synbiohub.org/public/igem/BBa_K1660004/annotation2445885/range2445885/1
type: Range
persistent identity: range2445885
display id: range2445885
version: 1
direction: direction/forward
top level: BBa_K1660004
start: 835
end: 1560
orientation: inline

BBa_S05053
type: ComponentDefinition
persistent identity: BBa S05053
display id: BBa_S05053
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: S03419:B0015
creator: Muyuan Chen
created: 2012-09-17T11:00:00Z
modified: 2015-05-08T01:14:49Z
discontinued: false
dominant: false
group u list: _9_
m user id: 0
owner id: 12559
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_S03419
component: BBa_B0015
sequence annotation: BBa_S03419
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_S05053_sequence/1

BBa_S03419
type: Component
persistent identity: component2184396
display id: component2184396
version: 1
top level: BBa_S05053
access: public
definition: https://synbiohub.org/public/igem/BBa_S03419/1

BBa_B0015
type: Component
persistent identity: component2184403
display id: component2184403
version: 1
top level: BBa_S05053
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_S03419
type: SequenceAnnotation
persistent identity: annotation2184396
display id: annotation2184396
version: 1
top level: BBa_S05053
location: https://synbiohub.org/public/igem/BBa_S05053/annotation2184396/range2184396/1
component: BBa_S03419

https://synbiohub.org/public/igem/BBa_S05053/annotation2184396/range2184396/1
type: Range
persistent identity: range2184396
display id: range2184396
version: 1
direction: direction/reverse
top level: BBa_S05053
start: 1
end: 2281
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2184403
display id: annotation2184403
version: 1
top level: BBa_S05053
location: https://synbiohub.org/public/igem/BBa_S05053/annotation2184403/range2184403/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_S05053/annotation2184403/range2184403/1
type: Range
persistent identity: range2184403
display id: range2184403
version: 1
direction: direction/reverse
top level: BBa_S05053
start: 2290
end: 2418
orientation: inline

BBa_K786001
type: ComponentDefinition
persistent identity: BBa K786001
display id: BBa_K786001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Sensory rhodopsin II (SRII) with HtrII & Tsr, sensitive to blue-green light
creator: Leung Wai Tak
created: 2012-09-20T11:00:00Z
modified: 2015-05-08T01:13:22Z
discontinued: false
dominant: false
group u list: _1041_
m user id: 0
owner id: 14285
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Sensory rhodopsin II fusion with HtrII & Tsr, which is sensitive to blue-green light, with sensing spectra covering from 400-500 nm. We used it for sensing blur-green light to achieve phototaxis and switch for downstream gene expression.
mutable notes: Pfam (version 26.0) was used to predict the domain of fusion protein. Linker was refereed and modified from previous research study of SRII fusion with HtrII.
mutable provenance: From genomic sequence of bacterial N. pharaonis (DSM 2160) and E.coli K12.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
sequence annotation: Constitutive promoter BBa_J23100
sequence annotation: HtrII
sequence annotation: RBS 1.0
sequence annotation: linker
sequence annotation: Sensory Rhodopsin II
sequence annotation: B0015
sequence annotation: BamHI for C-terminal fusion
sequence annotation: Tsr
sequence annotation: HindIII for N-terminal fusin
sequence: https://synbiohub.org/public/igem/BBa_K786001_sequence/1

Constitutive promoter BBa_J23100
type: SequenceAnnotation
persistent identity: annotation2199664
display id: annotation2199664
version: 1
top level: BBa_K786001
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K786001/annotation2199664/range2199664/1

https://synbiohub.org/public/igem/BBa_K786001/annotation2199664/range2199664/1
type: Range
persistent identity: range2199664
display id: range2199664
version: 1
direction: direction/forward
top level: BBa_K786001
start: 1
end: 42
orientation: inline

HtrII
type: SequenceAnnotation
persistent identity: annotation2199670
display id: annotation2199670
version: 1
top level: BBa_K786001
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K786001/annotation2199670/range2199670/1

https://synbiohub.org/public/igem/BBa_K786001/annotation2199670/range2199670/1
type: Range
persistent identity: range2199670
display id: range2199670
version: 1
direction: direction/forward
top level: BBa_K786001
start: 812
end: 1210
orientation: inline

RBS 1.0
type: SequenceAnnotation
persistent identity: annotation2199665
display id: annotation2199665
version: 1
top level: BBa_K786001
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K786001/annotation2199665/range2199665/1

https://synbiohub.org/public/igem/BBa_K786001/annotation2199665/range2199665/1
type: Range
persistent identity: range2199665
display id: range2199665
version: 1
direction: direction/forward
top level: BBa_K786001
start: 43
end: 55
orientation: inline

linker
type: SequenceAnnotation
persistent identity: annotation2199668
display id: annotation2199668
version: 1
top level: BBa_K786001
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K786001/annotation2199668/range2199668/1

https://synbiohub.org/public/igem/BBa_K786001/annotation2199668/range2199668/1
type: Range
persistent identity: range2199668
display id: range2199668
version: 1
direction: direction/forward
top level: BBa_K786001
start: 785
end: 811
orientation: inline

Sensory Rhodopsin II
type: SequenceAnnotation
persistent identity: annotation2199667
display id: annotation2199667
version: 1
top level: BBa_K786001
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K786001/annotation2199667/range2199667/1

https://synbiohub.org/public/igem/BBa_K786001/annotation2199667/range2199667/1
type: Range
persistent identity: range2199667
display id: range2199667
version: 1
direction: direction/forward
top level: BBa_K786001
start: 68
end: 784
orientation: inline

B0015
type: SequenceAnnotation
persistent identity: annotation2199672
display id: annotation2199672
version: 1
top level: BBa_K786001
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K786001/annotation2199672/range2199672/1

https://synbiohub.org/public/igem/BBa_K786001/annotation2199672/range2199672/1
type: Range
persistent identity: range2199672
display id: range2199672
version: 1
direction: direction/forward
top level: BBa_K786001
start: 2105
end: 2233
orientation: inline

BamHI for C-terminal fusion
type: SequenceAnnotation
persistent identity: annotation2199669
display id: annotation2199669
version: 1
top level: BBa_K786001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K786001/annotation2199669/range2199669/1

https://synbiohub.org/public/igem/BBa_K786001/annotation2199669/range2199669/1
type: Range
persistent identity: range2199669
display id: range2199669
version: 1
direction: direction/forward
top level: BBa_K786001
start: 785
end: 790
orientation: inline

Tsr
type: SequenceAnnotation
persistent identity: annotation2199671
display id: annotation2199671
version: 1
top level: BBa_K786001
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K786001/annotation2199671/range2199671/1

https://synbiohub.org/public/igem/BBa_K786001/annotation2199671/range2199671/1
type: Range
persistent identity: range2199671
display id: range2199671
version: 1
direction: direction/forward
top level: BBa_K786001
start: 1210
end: 2070
orientation: inline

HindIII for N-terminal fusin
type: SequenceAnnotation
persistent identity: annotation2199666
display id: annotation2199666
version: 1
top level: BBa_K786001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K786001/annotation2199666/range2199666/1

https://synbiohub.org/public/igem/BBa_K786001/annotation2199666/range2199666/1
type: Range
persistent identity: range2199666
display id: range2199666
version: 1
direction: direction/forward
top level: BBa_K786001
start: 62
end: 67
orientation: inline

BBa_K1982005
type: ComponentDefinition
persistent identity: BBa K1982005
display id: BBa_K1982005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CRY2-VP64(Prokaryotic LACE system)
creator: Zexu Li
created: 2016-09-11T11:00:00Z
modified: 2016-10-18T10:38:55Z
discontinued: false
dominant: false
group u list: _2449_
m user id: 30176
owner id: 30176
owning group id: 9
status: status/Planning
bookmark: false
mutable description: The CRY2/CIBN interaction is entirely genetically encoded. The binding reverses within minutes in the dark, allowing rapid shutoff of transcription by placing samples in the dark. This fusion protein is for use in LACE(light-activated CRISPR/Cas9 effector) system, and a VP64 fused to its C terminus. To regulate DNA transcription by blue light, the system is based on CRY2/CIBN interaction in which a light-mediated protein interaction brings together two protein (tCas9 and an activation domain VP64) . If we remove the stimulation of blue light, dark reversion of CRY2 will dissociate the interaction with CIBN and shut off transcription.
mutable notes: no
mutable provenance: CRY2 and CIB1 are extracted from Arabidopsis thaliana. tCas9 is from GE Share company. VP64 is synthetic construct.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
sequence annotation: ATG
sequence annotation: VP16
sequence annotation: Linker
sequence annotation: HA
sequence annotation: FLAG
sequence annotation: SV40 NLS
sequence annotation: Prokaryotic rbs
sequence annotation: CRY2
sequence annotation: TAATAA
sequence: https://synbiohub.org/public/igem/BBa_K1982005_sequence/1

ATG
type: SequenceAnnotation
persistent identity: annotation2517263
display id: annotation2517263
version: 1
top level: BBa_K1982005
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1982005/annotation2517263/range2517263/1

https://synbiohub.org/public/igem/BBa_K1982005/annotation2517263/range2517263/1
type: Range
persistent identity: range2517263
display id: range2517263
version: 1
direction: direction/forward
top level: BBa_K1982005
start: 19
end: 21
orientation: inline

VP16
type: SequenceAnnotation
persistent identity: annotation2517267
display id: annotation2517267
version: 1
top level: BBa_K1982005
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1982005/annotation2517267/range2517267/1

https://synbiohub.org/public/igem/BBa_K1982005/annotation2517267/range2517267/1
type: Range
persistent identity: range2517267
display id: range2517267
version: 1
direction: direction/forward
top level: BBa_K1982005
start: 1915
end: 1953
orientation: inline

VP16
type: SequenceAnnotation
persistent identity: annotation2517266
display id: annotation2517266
version: 1
top level: BBa_K1982005
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1982005/annotation2517266/range2517266/1

https://synbiohub.org/public/igem/BBa_K1982005/annotation2517266/range2517266/1
type: Range
persistent identity: range2517266
display id: range2517266
version: 1
direction: direction/forward
top level: BBa_K1982005
start: 1876
end: 1914
orientation: inline

Linker
type: SequenceAnnotation
persistent identity: annotation2517270
display id: annotation2517270
version: 1
top level: BBa_K1982005
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1982005/annotation2517270/range2517270/1

https://synbiohub.org/public/igem/BBa_K1982005/annotation2517270/range2517270/1
type: Range
persistent identity: range2517270
display id: range2517270
version: 1
direction: direction/forward
top level: BBa_K1982005
start: 2032
end: 2037
orientation: inline

HA
type: SequenceAnnotation
persistent identity: annotation2517271
display id: annotation2517271
version: 1
top level: BBa_K1982005
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1982005/annotation2517271/range2517271/1

https://synbiohub.org/public/igem/BBa_K1982005/annotation2517271/range2517271/1
type: Range
persistent identity: range2517271
display id: range2517271
version: 1
direction: direction/forward
top level: BBa_K1982005
start: 2037
end: 2063
orientation: inline

FLAG
type: SequenceAnnotation
persistent identity: annotation2517272
display id: annotation2517272
version: 1
top level: BBa_K1982005
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1982005/annotation2517272/range2517272/1

https://synbiohub.org/public/igem/BBa_K1982005/annotation2517272/range2517272/1
type: Range
persistent identity: range2517272
display id: range2517272
version: 1
direction: direction/forward
top level: BBa_K1982005
start: 2071
end: 2094
orientation: inline

SV40 NLS
type: SequenceAnnotation
persistent identity: annotation2517265
display id: annotation2517265
version: 1
top level: BBa_K1982005
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1982005/annotation2517265/range2517265/1

https://synbiohub.org/public/igem/BBa_K1982005/annotation2517265/range2517265/1
type: Range
persistent identity: range2517265
display id: range2517265
version: 1
direction: direction/forward
top level: BBa_K1982005
start: 1855
end: 1875
orientation: inline

Prokaryotic rbs
type: SequenceAnnotation
persistent identity: annotation2517262
display id: annotation2517262
version: 1
top level: BBa_K1982005
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1982005/annotation2517262/range2517262/1

https://synbiohub.org/public/igem/BBa_K1982005/annotation2517262/range2517262/1
type: Range
persistent identity: range2517262
display id: range2517262
version: 1
direction: direction/forward
top level: BBa_K1982005
start: 1
end: 18
orientation: inline

VP16
type: SequenceAnnotation
persistent identity: annotation2517269
display id: annotation2517269
version: 1
top level: BBa_K1982005
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1982005/annotation2517269/range2517269/1

https://synbiohub.org/public/igem/BBa_K1982005/annotation2517269/range2517269/1
type: Range
persistent identity: range2517269
display id: range2517269
version: 1
direction: direction/forward
top level: BBa_K1982005
start: 1993
end: 2031
orientation: inline

CRY2
type: SequenceAnnotation
persistent identity: annotation2517264
display id: annotation2517264
version: 1
top level: BBa_K1982005
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1982005/annotation2517264/range2517264/1

https://synbiohub.org/public/igem/BBa_K1982005/annotation2517264/range2517264/1
type: Range
persistent identity: range2517264
display id: range2517264
version: 1
direction: direction/reverse
top level: BBa_K1982005
start: 22
end: 1854
orientation: inline

TAATAA
type: SequenceAnnotation
persistent identity: annotation2517273
display id: annotation2517273
version: 1
top level: BBa_K1982005
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1982005/annotation2517273/range2517273/1

https://synbiohub.org/public/igem/BBa_K1982005/annotation2517273/range2517273/1
type: Range
persistent identity: range2517273
display id: range2517273
version: 1
direction: direction/forward
top level: BBa_K1982005
start: 2095
end: 2100
orientation: inline

VP16
type: SequenceAnnotation
persistent identity: annotation2517268
display id: annotation2517268
version: 1
top level: BBa_K1982005
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1982005/annotation2517268/range2517268/1

https://synbiohub.org/public/igem/BBa_K1982005/annotation2517268/range2517268/1
type: Range
persistent identity: range2517268
display id: range2517268
version: 1
direction: direction/forward
top level: BBa_K1982005
start: 1954
end: 1992
orientation: inline

BBa_K191009
type: ComponentDefinition
persistent identity: BBa K191009
display id: BBa_K191009
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LOVTAP (ILE427PHE)
creator: Le Thanh Tu NGUYEN
created: 2009-10-18T11:00:00Z
modified: 2015-05-08T01:11:15Z
discontinued: false
dominant: false
group u list: _287_
m user id: 0
owner id: 4198
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: See part BBa_K191005 for information on fused protein LOVTAP. This part is a mutated version of LOVTAP: amino acid #427 ILE is mutated into PHE
mutable notes: Mutagenesis kit of Stratagene was used
mutable provenance: Site specific mutation kit.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K191009_sequence/1

BBa_K106010
type: ComponentDefinition
persistent identity: BBa K106010
display id: BBa_K106010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PhyB, Aar1 AB part
creator: Willis Wong
created: 2008-10-22T11:00:00Z
modified: 2015-05-08T01:08:58Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _226_
m user id: 0
owner id: 3412
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This is a coding sequence for Pif3 gene with AB ends for Aar1 cloning. Binds with PhyB at 720nm light and unbinds at 660nm. Fuses only at the N-terminal only.
mutable notes: As a BD part, there is no ATG, and the stop codon is included. For more information on AarI cloning, please refer to the UCSF 2008 iGEM page. Fuses only at the N-terminal only.
mutable provenance: Arabidopsis thaliana
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K106010_sequence/1

BBa_K1982009
type: ComponentDefinition
persistent identity: BBa K1982009
display id: BBa_K1982009
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Eukaryotic Cryptochrome 2 (CRY2) ( a blue light stimulated photoreceptor)
creator: Zexu Li
created: 2016-10-08T11:00:00Z
modified: 2016-10-09T12:15:06Z
discontinued: false
dominant: false
group u list: _2449_
m user id: 30176
owner id: 30176
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Cryptochrome 2 (CRY2) is a blue light stimulated photoreceptor, when exposed to blue light, it would interact withthe N-terminal fragment of CIB1 (CIBN) . This part is full-length CRY2 CDS. Optogenetic systems enable precise spatial and temporal control of cell behavior. A light-activated CRISPR/Cas9 effector (LACE) system that induces transcription of endogenous genes in the presence of blue light.This was accomplished by fusing the light-inducible heterodimerizing proteins CRY2 and CIB1 to a transactivation domain and the catalytically inactive tCas9, respectively. The versatile LACE system can be easily directed to new DNA sequences for the dynamic regulation of endogenous genes[1].
mutable notes: mutant EcoR 1 : TGTTGGGAATTc--->TGTTGGGAATTg
mutable provenance: The light-inducible heterodimerizering proteins CRY2 and CIB1 are both from Arabidopsis thaliana.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Eukaryotic rbs
sequence annotation: atg
sequence: https://synbiohub.org/public/igem/BBa_K1982009_sequence/1

Eukaryotic rbs
type: SequenceAnnotation
persistent identity: annotation2488673
display id: annotation2488673
version: 1
top level: BBa_K1982009
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1982009/annotation2488673/range2488673/1

https://synbiohub.org/public/igem/BBa_K1982009/annotation2488673/range2488673/1
type: Range
persistent identity: range2488673
display id: range2488673
version: 1
direction: direction/forward
top level: BBa_K1982009
start: 1
end: 18
orientation: inline

atg
type: SequenceAnnotation
persistent identity: annotation2488672
display id: annotation2488672
version: 1
top level: BBa_K1982009
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1982009/annotation2488672/range2488672/1

https://synbiohub.org/public/igem/BBa_K1982009/annotation2488672/range2488672/1
type: Range
persistent identity: range2488672
display id: range2488672
version: 1
direction: direction/forward
top level: BBa_K1982009
start: 19
end: 21
orientation: inline

BBa_K191008
type: ComponentDefinition
persistent identity: BBa K191008
display id: BBa_K191008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter LacI - RBS - LovTAP(ILE427PHE) - Term
creator: Le Thanh Tu NGUYEN
created: 2009-10-18T11:00:00Z
modified: 2015-05-08T01:11:15Z
discontinued: false
dominant: false
experience: experience/None
group u list: _287_
m user id: 0
owner id: 4198
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: Complete coding sequence for mutated protein LOVTAP (mutation site: a.a #427 ILE is changed into PHE), with inducible promoter, RBS site and terminator.
mutable notes: Mutagenesis kit of Stratagene was used.
mutable provenance: Site specific mutation on plasmid with part BBa_K191003
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_B0012
component: BBa_R0010
component: BBa_K191009
component: BBa_B0030
component: BBa_B0010
sequence annotation: BBa_K191009
sequence annotation: BBa_B0030
sequence annotation: BBa_R0010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_K191008_sequence/1

BBa_B0012
type: Component
persistent identity: component2222447
display id: component2222447
version: 1
top level: BBa_K191008
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_R0010
type: Component
persistent identity: component2222434
display id: component2222434
version: 1
top level: BBa_K191008
access: public
definition: https://synbiohub.org/public/igem/BBa_R0010/1

BBa_K191009
type: Component
persistent identity: component2222444
display id: component2222444
version: 1
top level: BBa_K191008
access: public
definition: BBa_K191009

BBa_B0030
type: Component
persistent identity: component2222442
display id: component2222442
version: 1
top level: BBa_K191008
access: public
definition: https://synbiohub.org/public/igem/BBa_B0030/1

BBa_B0010
type: Component
persistent identity: component2222445
display id: component2222445
version: 1
top level: BBa_K191008
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_K191009
type: SequenceAnnotation
persistent identity: annotation2222444
display id: annotation2222444
version: 1
top level: BBa_K191008
location: https://synbiohub.org/public/igem/BBa_K191008/annotation2222444/range2222444/1
component: BBa_K191009

https://synbiohub.org/public/igem/BBa_K191008/annotation2222444/range2222444/1
type: Range
persistent identity: range2222444
display id: range2222444
version: 1
direction: direction/reverse
top level: BBa_K191008
start: 230
end: 916
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation2222442
display id: annotation2222442
version: 1
top level: BBa_K191008
location: https://synbiohub.org/public/igem/BBa_K191008/annotation2222442/range2222442/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_K191008/annotation2222442/range2222442/1
type: Range
persistent identity: range2222442
display id: range2222442
version: 1
direction: direction/forward
top level: BBa_K191008
start: 209
end: 223
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation2222434
display id: annotation2222434
version: 1
top level: BBa_K191008
location: https://synbiohub.org/public/igem/BBa_K191008/annotation2222434/range2222434/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_K191008/annotation2222434/range2222434/1
type: Range
persistent identity: range2222434
display id: range2222434
version: 1
direction: direction/forward
top level: BBa_K191008
start: 1
end: 200
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2222445
display id: annotation2222445
version: 1
top level: BBa_K191008
location: https://synbiohub.org/public/igem/BBa_K191008/annotation2222445/range2222445/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K191008/annotation2222445/range2222445/1
type: Range
persistent identity: range2222445
display id: range2222445
version: 1
direction: direction/forward
top level: BBa_K191008
start: 925
end: 1004
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2222447
display id: annotation2222447
version: 1
top level: BBa_K191008
location: https://synbiohub.org/public/igem/BBa_K191008/annotation2222447/range2222447/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K191008/annotation2222447/range2222447/1
type: Range
persistent identity: range2222447
display id: range2222447
version: 1
direction: direction/forward
top level: BBa_K191008
start: 1013
end: 1053
orientation: inline

BBa_I15010
type: ComponentDefinition
persistent identity: BBa I15010
display id: BBa_I15010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: cph8 (Cph1/EnvZ fusion)
creator: Jeff Tabor
created: 2004-09-19T11:00:00Z
modified: 2015-08-31T04:07:38Z
discontinued: false
dominant: false
experience: experience/None
group u list: _5_
m user id: 0
owner id: 88
owning group id: 7
sample status: In stock
status: status/Available
bookmark: true
mutable description: Chimeric Cph1 light receptor/EnvZ protein.
mutable notes: Silent mutation at base 108 (G-A) to remove PstI site
mutable provenance: Voigt Lab via Anselm Levskaya
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Cph8
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_I15010_sequence/1

Cph8
type: SequenceAnnotation
persistent identity: annotation1891613
display id: annotation1891613
version: 1
top level: BBa_I15010
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_I15010/annotation1891613/range1891613/1

https://synbiohub.org/public/igem/BBa_I15010/annotation1891613/range1891613/1
type: Range
persistent identity: range1891613
display id: range1891613
version: 1
direction: direction/forward
top level: BBa_I15010
start: 1
end: 2238
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2214031
display id: annotation2214031
version: 1
top level: BBa_I15010
role: SO:0000807
role: feature/barcode
location: https://synbiohub.org/public/igem/BBa_I15010/annotation2214031/range2214031/1

https://synbiohub.org/public/igem/BBa_I15010/annotation2214031/range2214031/1
type: Range
persistent identity: range2214031
display id: range2214031
version: 1
direction: direction/forward
top level: BBa_I15010
start: 2239
end: 2263
orientation: inline

BBa_K1660007
type: ComponentDefinition
persistent identity: BBa K1660007
display id: BBa_K1660007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PompR-6N-gp35-Terminator
creator: Dai Yuanyi
created: 2015-09-07T11:00:00Z
modified: 2015-09-08T12:46:10Z
discontinued: false
dominant: false
group u list: _2078_
m user id: 26933
owner id: 26933
owning group id: 9
status: status/Planning
bookmark: false
mutable description: PompR is a OmpR-controlled promoter which can be positively regulated by phosphorylated OmpR. This promoter is taken from the upstream region of ompC. Phosphorylated OmpR binds to the three operator sites and activates transcription. We insert PompR(BBa_R0082) sequences into the upstream region of our target gene gp35 through restriction-ligation method, which together is connected to vector PSB1C3 afterwards, and therefore we are able to regulate the expression of in through the control of light. Bxb1 gp35 is a serine integrase in Mycobacterium phage Bxb1. This intergrase can exclusively catalyze site-specific recombination. The dismiss of light singal can drive the expression of integrase, which alone can set a DNA sequence(there it means the promoter) flanked by oppositional attB and attP sites, thereby produce an inverted sequence flanked by attL and attR sites. As a consequence, the promotor moves on to express the toxic protein instead of the attractant. We insert the gp35 gene into the downstream region of the PompR promotor by means of 3A synthesis so that the expression of integrase can be regulated by light. In addition, we add a 6N promotor between PompR and gp35 to decrease basal integrase expression above flipping threshold level which could lower the efficiency of int translation. Meanwhile, we insert the recombined sites of attB and attP into the flanking sequences of our constitutive promotor (BBa_J23110 ) and so far we accomplish our reverse promotor system.
mutable notes: we add a 6N promotor between PompR and gp35 to decrease basal integrase expression above flipping threshold level which could lower the efficiency of int translation.
mutable provenance: It comes from the register.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1433008
component: BBa_R0082
sequence annotation: BBa_R0082
sequence annotation: BBa_K1433008
sequence: https://synbiohub.org/public/igem/BBa_K1660007_sequence/1

BBa_K1433008
type: Component
persistent identity: component2446523
display id: component2446523
version: 1
top level: BBa_K1660007
access: public
definition: https://synbiohub.org/public/igem/BBa_K1433008/1

BBa_R0082
type: Component
persistent identity: component2446518
display id: component2446518
version: 1
top level: BBa_K1660007
access: public
definition: https://synbiohub.org/public/igem/BBa_R0082/1

BBa_R0082
type: SequenceAnnotation
persistent identity: annotation2446518
display id: annotation2446518
version: 1
top level: BBa_K1660007
location: https://synbiohub.org/public/igem/BBa_K1660007/annotation2446518/range2446518/1
component: BBa_R0082

https://synbiohub.org/public/igem/BBa_K1660007/annotation2446518/range2446518/1
type: Range
persistent identity: range2446518
display id: range2446518
version: 1
direction: direction/reverse
top level: BBa_K1660007
start: 1
end: 108
orientation: inline

BBa_K1433008
type: SequenceAnnotation
persistent identity: annotation2446523
display id: annotation2446523
version: 1
top level: BBa_K1660007
location: https://synbiohub.org/public/igem/BBa_K1660007/annotation2446523/range2446523/1
component: BBa_K1433008

https://synbiohub.org/public/igem/BBa_K1660007/annotation2446523/range2446523/1
type: Range
persistent identity: range2446523
display id: range2446523
version: 1
direction: direction/reverse
top level: BBa_K1660007
start: 117
end: 1773
orientation: inline

BBa_K559001
type: ComponentDefinition
persistent identity: BBa K559001
display id: BBa_K559001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Halorhodopsin under T7 promoter
creator: Jacky, Fong Chuen, Loo
created: 2011-09-28T11:00:00Z
modified: 2015-05-08T01:12:41Z
discontinued: false
dominant: false
experience: experience/None
group u list: _727_
m user id: 0
owner id: 6123
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Halorhodopsin under constitute T7 promoter to regulate the gene transcription to be maximized and no restrict to other regulatory factor
mutable notes: The coding sequence of N.pharaonis J05199.1 is selected from 61nt to 700nt and the T7 promotor placed in front by 3A assembly
mutable provenance: Halorhodopsin come from N.pharaonis, gene bank J05199.1
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: RBS
sequence annotation: T7 promotor
sequence annotation: Halorhodopsin
sequence annotation: lac repressor
sequence: https://synbiohub.org/public/igem/BBa_K559001_sequence/1

RBS
type: SequenceAnnotation
persistent identity: annotation2177555
display id: annotation2177555
version: 1
top level: BBa_K559001
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K559001/annotation2177555/range2177555/1

https://synbiohub.org/public/igem/BBa_K559001/annotation2177555/range2177555/1
type: Range
persistent identity: range2177555
display id: range2177555
version: 1
direction: direction/forward
top level: BBa_K559001
start: 66
end: 77
orientation: inline

T7 promotor
type: SequenceAnnotation
persistent identity: annotation2177551
display id: annotation2177551
version: 1
top level: BBa_K559001
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K559001/annotation2177551/range2177551/1

https://synbiohub.org/public/igem/BBa_K559001/annotation2177551/range2177551/1
type: Range
persistent identity: range2177551
display id: range2177551
version: 1
direction: direction/forward
top level: BBa_K559001
start: 1
end: 16
orientation: inline

Halorhodopsin
type: SequenceAnnotation
persistent identity: annotation2177554
display id: annotation2177554
version: 1
top level: BBa_K559001
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K559001/annotation2177554/range2177554/1

https://synbiohub.org/public/igem/BBa_K559001/annotation2177554/range2177554/1
type: Range
persistent identity: range2177554
display id: range2177554
version: 1
direction: direction/forward
top level: BBa_K559001
start: 89
end: 728
orientation: inline

lac repressor
type: SequenceAnnotation
persistent identity: annotation2177553
display id: annotation2177553
version: 1
top level: BBa_K559001
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K559001/annotation2177553/range2177553/1

https://synbiohub.org/public/igem/BBa_K559001/annotation2177553/range2177553/1
type: Range
persistent identity: range2177553
display id: range2177553
version: 1
direction: direction/forward
top level: BBa_K559001
start: 22
end: 42
orientation: inline

BBa_K1587008
type: ComponentDefinition
persistent identity: BBa K1587008
display id: BBa_K1587008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Red light biosensor
creator: Alexandre Le Scornet
created: 2015-09-15T11:00:00Z
modified: 2015-09-17T02:51:05Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _2004_
m user id: 24598
owner id: 24598
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Strong promoter BBa_J23119. Chimeric Red light receptor cph8 BBa_I15010 (cph1-envZ) and proteins involved in phycobilin biosynthesis ho1 (BBa_I15008), pcyA (BBa_I15009).
mutable notes: No information
mutable provenance: Sequences are from Cyanobacteria : - Cph1 domain - ho1 BBa_I15008 - pcyA BBa_I15009
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
sequence annotation: cph8
sequence annotation: rbs
sequence annotation: Strong promoter
sequence: https://synbiohub.org/public/igem/BBa_K1587008_sequence/1

cph8
type: SequenceAnnotation
persistent identity: annotation2466982
display id: annotation2466982
version: 1
top level: BBa_K1587008
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1587008/annotation2466982/range2466982/1

https://synbiohub.org/public/igem/BBa_K1587008/annotation2466982/range2466982/1
type: Range
persistent identity: range2466982
display id: range2466982
version: 1
direction: direction/forward
top level: BBa_K1587008
start: 51
end: 2288
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation2466981
display id: annotation2466981
version: 1
top level: BBa_K1587008
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1587008/annotation2466981/range2466981/1

https://synbiohub.org/public/igem/BBa_K1587008/annotation2466981/range2466981/1
type: Range
persistent identity: range2466981
display id: range2466981
version: 1
direction: direction/forward
top level: BBa_K1587008
start: 36
end: 50
orientation: inline

Strong promoter
type: SequenceAnnotation
persistent identity: annotation2466980
display id: annotation2466980
version: 1
top level: BBa_K1587008
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1587008/annotation2466980/range2466980/1

https://synbiohub.org/public/igem/BBa_K1587008/annotation2466980/range2466980/1
type: Range
persistent identity: range2466980
display id: range2466980
version: 1
direction: direction/forward
top level: BBa_K1587008
start: 1
end: 35
orientation: inline

BBa_K786004
type: ComponentDefinition
persistent identity: BBa K786004
display id: BBa_K786004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Red light triggering phototaxis using chp8
creator: Leung Wai Tak
created: 2012-09-20T11:00:00Z
modified: 2015-05-08T01:13:22Z
discontinued: true
dominant: false
experience: experience/None
group u list: _1041_
m user id: 0
owner id: 14285
owning group id: 9
sample status: Discontinued
status: status/Deleted
bookmark: false
mutable description: Red light triggering phototaxis using chp8, which can sense the red light from 600-700 nm.
mutable notes: 6 poly-His tag was positioned at C-terminal of the protein.
mutable provenance: The sequence of chp8 is from previous biobrick.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
sequence annotation: RBS 1.0
sequence annotation: poly-HIS tag
sequence annotation: Constitutive promoter BBa_J23100
sequence annotation: chp8 protein
sequence: https://synbiohub.org/public/igem/BBa_K786004_sequence/1

RBS 1.0
type: SequenceAnnotation
persistent identity: annotation2190188
display id: annotation2190188
version: 1
top level: BBa_K786004
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K786004/annotation2190188/range2190188/1

https://synbiohub.org/public/igem/BBa_K786004/annotation2190188/range2190188/1
type: Range
persistent identity: range2190188
display id: range2190188
version: 1
direction: direction/forward
top level: BBa_K786004
start: 51
end: 62
orientation: inline

poly-HIS tag
type: SequenceAnnotation
persistent identity: annotation2190190
display id: annotation2190190
version: 1
top level: BBa_K786004
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K786004/annotation2190190/range2190190/1

https://synbiohub.org/public/igem/BBa_K786004/annotation2190190/range2190190/1
type: Range
persistent identity: range2190190
display id: range2190190
version: 1
direction: direction/forward
top level: BBa_K786004
start: 2301
end: 2321
orientation: inline

Constitutive promoter BBa_J23100
type: SequenceAnnotation
persistent identity: annotation2190187
display id: annotation2190187
version: 1
top level: BBa_K786004
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K786004/annotation2190187/range2190187/1

https://synbiohub.org/public/igem/BBa_K786004/annotation2190187/range2190187/1
type: Range
persistent identity: range2190187
display id: range2190187
version: 1
direction: direction/forward
top level: BBa_K786004
start: 1
end: 50
orientation: inline

chp8 protein
type: SequenceAnnotation
persistent identity: annotation2190189
display id: annotation2190189
version: 1
top level: BBa_K786004
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K786004/annotation2190189/range2190189/1

https://synbiohub.org/public/igem/BBa_K786004/annotation2190189/range2190189/1
type: Range
persistent identity: range2190189
display id: range2190189
version: 1
direction: direction/forward
top level: BBa_K786004
start: 69
end: 2321
orientation: inline

BBa_K360121
type: ComponentDefinition
persistent identity: BBa K360121
display id: BBa_K360121
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS + LovTAP Photosensor + terminator
creator: Claudia Ivonne Hernandez Armenta
created: 2010-09-08T11:00:00Z
modified: 2015-05-08T01:12:12Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _485_
m user id: 0
owner id: 6618
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This photoreceptor was assembled by Strickland et al., and consists of a LOV (light-oxygen-voltage) domain of Avena sativa phototropin1 (AsLOV2) that senses blue light, fused to the trpR- DNA binding domain of the transcription factor trp repressor. The resulting protein is named LovTAP: LOV- and tryptophan-activated protein. LOV domains bind a flavin-mononucleotide (FMN) or flavin-adenine-dinucleotide (FAD) cofactor, which are used in a wide variety of metabolic pathways as cofactors in redox reactions and are available in most organisms. The cofactor has a broad absorption spectrum, with a maximum at 450 nm. Besides, the core of the LOV domain is often flanked by amino- or carboxy-terminal helices, termed A???α and Jα, respectively In the LovTAP construction, AsLOV2 domain via its carboxyl-terminal Jα ???helix was ligated to an amino-terminal truncation of TrpR. The resulting protein has a domain-domain overlap with a shared helix. Thus, photoexcitation would change the conformation of the protein, in turn changing the stability of the shared-helix-domain contacts. Under the presence of light, absorption of a photon leads to the formation of a covalent adduct between the flavin mononucleotide (FMN) cofactor and a conserved cysteine residue in the AsLOV2 domain, which results in conformational rearrangements in LovTAP. This change impacts the affinity of the shared helix for the two domains: disrupting the contacts between the shared helix and the LOV domain and enabling the association of the shared helix with the TrpR domain, which establishes DNA-binding affinity and LovTAP can then bind DNA as an homodimer, repressing the transcription of the genes downstream of its binding sites. In the dark, when the shared helix contacts the LOV domain, the TrpR domain's DNA-binding affinity decreases and LovTAP is in an inactive conformation.
mutable notes: We decided to synthesize a new LovTAP part, that in comparison with the Part:BBa_K191003 that is already at the registry, has the following differences: 1. The 2 PstI restriction sites were removed from the coding region of LovTap. 2. We included a punctual mutation to change the ILE427 by a PHE427, as was proposed by the model results of the team iGEM09_EPF-Lausanne. With this mutation LovTAP should react faster and the conformational change should be more stable (the protein stays in the active form for longer, under light induction). 3. The part does not include a promoter. We eliminated the inverting regulator sensitive to LacI and CAP protein(Part:BBa_R0010). According to the report of EPF-Laussane team, the expression levels of LovTAP under the inverting regulator, doesn??t seem to show differences to the induction with IPTG. Thus, we decided to remove it and as we know that the level expression of LovTAP must be low (because at high levels of expresion, there is unspecific regulation under darkness and light states), we plan to test weak promoters to choose the best option and include it in the construction. 4. The RBS was changed from a strong (Part: BBa_B0030) to a medium strength (Part:BBa_B0032), thus expecting lower levels of lovTAP protein. 5. The stop codon tga was changed for two taa.
mutable provenance: Chiameric protein assembled by Strickland et al. Reference: Strickland, D., Moffat, K., & Sosnick, T. (2008). Light-activated DNA binding in a designed allosteric protein. Proceedings of the National Academy of Sciences, 105(31), 10709. National Acad Sciences. Retrieved from http://www.pnas.org/content/105/31/10709.full.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Signalling
role: SO:0000804
sequence annotation: Terminator B0010
sequence annotation: RBS B0032
sequence annotation: Terminator B0012
sequence annotation: AAA
sequence annotation: stop codon
sequence annotation: LovTAP K360022
sequence: https://synbiohub.org/public/igem/BBa_K360121_sequence/1

Terminator B0010
type: SequenceAnnotation
persistent identity: annotation2091649
display id: annotation2091649
version: 1
top level: BBa_K360121
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K360121/annotation2091649/range2091649/1

https://synbiohub.org/public/igem/BBa_K360121/annotation2091649/range2091649/1
type: Range
persistent identity: range2091649
display id: range2091649
version: 1
direction: direction/forward
top level: BBa_K360121
start: 725
end: 771
orientation: inline

RBS B0032
type: SequenceAnnotation
persistent identity: annotation2091646
display id: annotation2091646
version: 1
top level: BBa_K360121
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K360121/annotation2091646/range2091646/1

https://synbiohub.org/public/igem/BBa_K360121/annotation2091646/range2091646/1
type: Range
persistent identity: range2091646
display id: range2091646
version: 1
direction: direction/forward
top level: BBa_K360121
start: 1
end: 13
orientation: inline

Terminator B0012
type: SequenceAnnotation
persistent identity: annotation2091650
display id: annotation2091650
version: 1
top level: BBa_K360121
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K360121/annotation2091650/range2091650/1

https://synbiohub.org/public/igem/BBa_K360121/annotation2091650/range2091650/1
type: Range
persistent identity: range2091650
display id: range2091650
version: 1
direction: direction/forward
top level: BBa_K360121
start: 804
end: 830
orientation: inline

AAA
type: SequenceAnnotation
persistent identity: annotation2091651
display id: annotation2091651
version: 1
top level: BBa_K360121
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_K360121/annotation2091651/range2091651/1

https://synbiohub.org/public/igem/BBa_K360121/annotation2091651/range2091651/1
type: Range
persistent identity: range2091651
display id: range2091651
version: 1
direction: direction/forward
top level: BBa_K360121
start: 831
end: 844
orientation: inline

stop codon
type: SequenceAnnotation
persistent identity: annotation2091647
display id: annotation2091647
version: 1
top level: BBa_K360121
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K360121/annotation2091647/range2091647/1

https://synbiohub.org/public/igem/BBa_K360121/annotation2091647/range2091647/1
type: Range
persistent identity: range2091647
display id: range2091647
version: 1
direction: direction/forward
top level: BBa_K360121
start: 702
end: 706
orientation: inline

LovTAP K360022
type: SequenceAnnotation
persistent identity: annotation2091648
display id: annotation2091648
version: 1
top level: BBa_K360121
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K360121/annotation2091648/range2091648/1

https://synbiohub.org/public/igem/BBa_K360121/annotation2091648/range2091648/1
type: Range
persistent identity: range2091648
display id: range2091648
version: 1
direction: direction/forward
top level: BBa_K360121
start: 18
end: 701
orientation: inline

BBa_K191002
type: ComponentDefinition
persistent identity: BBa K191002
display id: BBa_K191002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LovTAP - Term
creator: Le Thanh Tu NGUYEN
created: 2009-10-10T11:00:00Z
modified: 2015-05-08T01:11:15Z
discontinued: false
dominant: false
experience: experience/None
group u list: _287_
m user id: 0
owner id: 4198
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Coding sequence of a fused protein (LOV means light, oxygen, and voltage,represent a light sensitive protein domain whereas TAP means tryptophan-activated protein) followed by a terminator.
mutable notes: LOVTap sequence contains 2 Pst1 restriction sites
mutable provenance: Kindly provided by the authors of : Light-activated DNA binding in a designed allosteric protein; Strickland et al., Proceedings of the National Academy of Sciences (2008) vol. 105 (31) pp. 10709
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_B0015
component: BBa_K191006
sequence annotation: BBa_B0015
sequence annotation: BBa_K191006
sequence: https://synbiohub.org/public/igem/BBa_K191002_sequence/1

BBa_B0015
type: Component
persistent identity: component2219505
display id: component2219505
version: 1
top level: BBa_K191002
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_K191006
type: Component
persistent identity: component2219498
display id: component2219498
version: 1
top level: BBa_K191002
access: public
definition: LovTAP

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2219505
display id: annotation2219505
version: 1
top level: BBa_K191002
location: https://synbiohub.org/public/igem/BBa_K191002/annotation2219505/range2219505/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K191002/annotation2219505/range2219505/1
type: Range
persistent identity: range2219505
display id: range2219505
version: 1
direction: direction/reverse
top level: BBa_K191002
start: 696
end: 824
orientation: inline

BBa_K191006
type: SequenceAnnotation
persistent identity: annotation2219498
display id: annotation2219498
version: 1
top level: BBa_K191002
location: https://synbiohub.org/public/igem/BBa_K191002/annotation2219498/range2219498/1
component: BBa_K191006

https://synbiohub.org/public/igem/BBa_K191002/annotation2219498/range2219498/1
type: Range
persistent identity: range2219498
display id: range2219498
version: 1
direction: direction/reverse
top level: BBa_K191002
start: 1
end: 687
orientation: inline

BBa_M36921
type: ComponentDefinition
persistent identity: BBa M36921
display id: BBa_M36921
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Zinc Sensor
creator: Leah Chase
created: 2014-10-21T11:00:00Z
modified: 2015-05-08T01:14:07Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _848_
m user id: 0
owner id: 24110
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: This composite part contains a promoter and ribosome-binding-site, a gene for ZntR, a terminator, and a promoter for ZntA. In the absence of Zinc, the ZntR protein binds to the ZntA binding site. In the presence of zinc, ZntR undergoes a conformation, does not attach to the binding site, and allows for transcription of ZntA. Essentially, this part acts as a sensor for the presence of zinc.
mutable notes: We had to enter silent mutations to change the DNA sequence without affecting the amino acid sequence because there were palindromes greater than 10 bp in every basic part.
mutable provenance: The genomic sequence comes from parts of an E. Coli protein (ZntR).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_J61101
component: BBa_M36919
component: BBa_B0010
component: BBa_J23108
component: BBa_K190016
sequence annotation: BBa_J61101
sequence annotation: BBa_J23108
sequence annotation: BBa_M36919
sequence annotation: BBa_B0010
sequence annotation: BBa_K190016
sequence: https://synbiohub.org/public/igem/BBa_M36921_sequence/1

BBa_J61101
type: Component
persistent identity: component2429148
display id: component2429148
version: 1
top level: BBa_M36921
access: public
definition: https://synbiohub.org/public/igem/BBa_J61101/1

BBa_M36919
type: Component
persistent identity: component2429149
display id: component2429149
version: 1
top level: BBa_M36921
access: public
definition: https://synbiohub.org/public/igem/BBa_M36919/1

BBa_B0010
type: Component
persistent identity: component2429150
display id: component2429150
version: 1
top level: BBa_M36921
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_J23108
type: Component
persistent identity: component2429147
display id: component2429147
version: 1
top level: BBa_M36921
access: public
definition: https://synbiohub.org/public/igem/BBa_J23108/1

BBa_K190016
type: Component
persistent identity: component2429154
display id: component2429154
version: 1
top level: BBa_M36921
access: public
definition: https://synbiohub.org/public/igem/BBa_K190016/1

BBa_J61101
type: SequenceAnnotation
persistent identity: annotation2429148
display id: annotation2429148
version: 1
top level: BBa_M36921
location: https://synbiohub.org/public/igem/BBa_M36921/annotation2429148/range2429148/1
component: BBa_J61101

https://synbiohub.org/public/igem/BBa_M36921/annotation2429148/range2429148/1
type: Range
persistent identity: range2429148
display id: range2429148
version: 1
direction: direction/reverse
top level: BBa_M36921
start: 44
end: 55
orientation: inline

BBa_J23108
type: SequenceAnnotation
persistent identity: annotation2429147
display id: annotation2429147
version: 1
top level: BBa_M36921
location: https://synbiohub.org/public/igem/BBa_M36921/annotation2429147/range2429147/1
component: BBa_J23108

https://synbiohub.org/public/igem/BBa_M36921/annotation2429147/range2429147/1
type: Range
persistent identity: range2429147
display id: range2429147
version: 1
direction: direction/reverse
top level: BBa_M36921
start: 1
end: 35
orientation: inline

BBa_M36919
type: SequenceAnnotation
persistent identity: annotation2429149
display id: annotation2429149
version: 1
top level: BBa_M36921
location: https://synbiohub.org/public/igem/BBa_M36921/annotation2429149/range2429149/1
component: BBa_M36919

https://synbiohub.org/public/igem/BBa_M36921/annotation2429149/range2429149/1
type: Range
persistent identity: range2429149
display id: range2429149
version: 1
direction: direction/reverse
top level: BBa_M36921
start: 62
end: 475
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2429150
display id: annotation2429150
version: 1
top level: BBa_M36921
location: https://synbiohub.org/public/igem/BBa_M36921/annotation2429150/range2429150/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_M36921/annotation2429150/range2429150/1
type: Range
persistent identity: range2429150
display id: range2429150
version: 1
direction: direction/forward
top level: BBa_M36921
start: 484
end: 563
orientation: inline

BBa_K190016
type: SequenceAnnotation
persistent identity: annotation2429154
display id: annotation2429154
version: 1
top level: BBa_M36921
location: https://synbiohub.org/public/igem/BBa_M36921/annotation2429154/range2429154/1
component: BBa_K190016

https://synbiohub.org/public/igem/BBa_M36921/annotation2429154/range2429154/1
type: Range
persistent identity: range2429154
display id: range2429154
version: 1
direction: direction/reverse
top level: BBa_M36921
start: 572
end: 640
orientation: inline

BBa_I15009
type: ComponentDefinition
persistent identity: BBa I15009
display id: BBa_I15009
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: phycocyanobilin:ferredoxin oxidoreductase (PcyA) from synechocystis
creator: Jeff Tabor
created: 2004-09-19T11:00:00Z
modified: 2015-08-31T04:07:38Z
discontinued: false
dominant: false
experience: experience/None
group u list: _5_
m user id: 0
owner id: 88
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: Second of two required phycocyanobilin biosynthetic genes.
mutable provenance: Voigt Lab via Anselm Levskaya
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: PcyA
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_I15009_sequence/1

PcyA
type: SequenceAnnotation
persistent identity: annotation1891612
display id: annotation1891612
version: 1
top level: BBa_I15009
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_I15009/annotation1891612/range1891612/1

https://synbiohub.org/public/igem/BBa_I15009/annotation1891612/range1891612/1
type: Range
persistent identity: range1891612
display id: range1891612
version: 1
direction: direction/forward
top level: BBa_I15009
start: 1
end: 750
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2214032
display id: annotation2214032
version: 1
top level: BBa_I15009
role: SO:0000807
role: feature/barcode
location: https://synbiohub.org/public/igem/BBa_I15009/annotation2214032/range2214032/1

https://synbiohub.org/public/igem/BBa_I15009/annotation2214032/range2214032/1
type: Range
persistent identity: range2214032
display id: range2214032
version: 1
direction: direction/forward
top level: BBa_I15009
start: 751
end: 775
orientation: inline

BBa_K1936001
type: ComponentDefinition
persistent identity: BBa K1936001
display id: BBa_K1936001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Tom5-eGFP-LOV2
creator: Marcel Dickmanns
created: 2016-10-12T11:00:00Z
modified: 2016-10-19T03:03:02Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2403_
m user id: 30478
owner id: 31678
owning group id: 9
status: status/Planning
bookmark: true
mutable description: ALERT !!!Under construction !!
mutable notes: ALERT !!!Under construction !!
mutable provenance: ALERT !!!Under construction !!
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Other
role: SO:0000110
sequence annotation: TOM5
sequence annotation: Linker
sequence annotation: LOV2
sequence annotation: eGFP
sequence: https://synbiohub.org/public/igem/BBa_K1936001_sequence/1

TOM5
type: SequenceAnnotation
persistent identity: annotation2521537
display id: annotation2521537
version: 1
top level: BBa_K1936001
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1936001/annotation2521537/range2521537/1

https://synbiohub.org/public/igem/BBa_K1936001/annotation2521537/range2521537/1
type: Range
persistent identity: range2521537
display id: range2521537
version: 1
direction: direction/forward
top level: BBa_K1936001
start: 1
end: 153
orientation: inline

Linker
type: SequenceAnnotation
persistent identity: annotation2521540
display id: annotation2521540
version: 1
top level: BBa_K1936001
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1936001/annotation2521540/range2521540/1

https://synbiohub.org/public/igem/BBa_K1936001/annotation2521540/range2521540/1
type: Range
persistent identity: range2521540
display id: range2521540
version: 1
direction: direction/forward
top level: BBa_K1936001
start: 907
end: 936
orientation: inline

LOV2
type: SequenceAnnotation
persistent identity: annotation2521541
display id: annotation2521541
version: 1
top level: BBa_K1936001
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1936001/annotation2521541/range2521541/1

https://synbiohub.org/public/igem/BBa_K1936001/annotation2521541/range2521541/1
type: Range
persistent identity: range2521541
display id: range2521541
version: 1
direction: direction/forward
top level: BBa_K1936001
start: 937
end: 1368
orientation: inline

Linker
type: SequenceAnnotation
persistent identity: annotation2521538
display id: annotation2521538
version: 1
top level: BBa_K1936001
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1936001/annotation2521538/range2521538/1

https://synbiohub.org/public/igem/BBa_K1936001/annotation2521538/range2521538/1
type: Range
persistent identity: range2521538
display id: range2521538
version: 1
direction: direction/forward
top level: BBa_K1936001
start: 154
end: 192
orientation: inline

eGFP
type: SequenceAnnotation
persistent identity: annotation2521539
display id: annotation2521539
version: 1
top level: BBa_K1936001
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1936001/annotation2521539/range2521539/1

https://synbiohub.org/public/igem/BBa_K1936001/annotation2521539/range2521539/1
type: Range
persistent identity: range2521539
display id: range2521539
version: 1
direction: direction/forward
top level: BBa_K1936001
start: 193
end: 906
orientation: inline

BBa_K772102
type: ComponentDefinition
persistent identity: BBa K772102
display id: BBa_K772102
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Halorhodopsin ready-to-use system
creator: Mustafa Elitok
created: 2012-09-25T11:00:00Z
modified: 2015-05-08T01:13:15Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1024_
m user id: 0
owner id: 9573
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Halorhodopsin is an inward-directed light-driven chloride ion pump originating from Halobacterium. It utilizes light to pump chloride ions against chloride concentration difference into cells from the environment. We added a constitutive working and regulatable pLac promoter in the upstream region of the part in order to use it in our design easily.
mutable notes: Please refer to http://partsregistry.org/Part:BBa_K559000:Design for more detail about halorhodopsin. Please refer to http://partsregistry.org/Part:BBa_J04500:Design for more detail about pLac promoter.
mutable provenance: Please refer to http://partsregistry.org/Part:BBa_K559000:Design, both of the genes are gathered from the Registry distribution.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K559010
component: BBa_J04500
sequence annotation: BBa_J04500
sequence annotation: BBa_K559010
sequence: https://synbiohub.org/public/igem/BBa_K772102_sequence/1

BBa_K559010
type: Component
persistent identity: component2202534
display id: component2202534
version: 1
top level: BBa_K772102
access: public
definition: BBa_K559010

BBa_J04500
type: Component
persistent identity: component2202521
display id: component2202521
version: 1
top level: BBa_K772102
access: public
definition: https://synbiohub.org/public/igem/BBa_J04500/1

BBa_J04500
type: SequenceAnnotation
persistent identity: annotation2202521
display id: annotation2202521
version: 1
top level: BBa_K772102
location: https://synbiohub.org/public/igem/BBa_K772102/annotation2202521/range2202521/1
component: BBa_J04500

https://synbiohub.org/public/igem/BBa_K772102/annotation2202521/range2202521/1
type: Range
persistent identity: range2202521
display id: range2202521
version: 1
direction: direction/reverse
top level: BBa_K772102
start: 1
end: 220
orientation: inline

BBa_K559010
type: SequenceAnnotation
persistent identity: annotation2202534
display id: annotation2202534
version: 1
top level: BBa_K772102
location: https://synbiohub.org/public/igem/BBa_K772102/annotation2202534/range2202534/1
component: BBa_K559010

https://synbiohub.org/public/igem/BBa_K772102/annotation2202534/range2202534/1
type: Range
persistent identity: range2202534
display id: range2202534
version: 1
direction: direction/reverse
top level: BBa_K772102
start: 229
end: 1059
orientation: inline

BBa_K1660006
type: ComponentDefinition
persistent identity: BBa K1660006
display id: BBa_K1660006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: J23100-B0034-cph8
creator: Dai Yuanyi
created: 2015-09-07T11:00:00Z
modified: 2015-09-08T12:31:43Z
discontinued: false
dominant: false
group u list: _2078_
m user id: 26933
owner id: 26933
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This is the light sensor Cph8 with a promoter and RBS.
mutable notes: We constructed it through pcr.
mutable provenance: It comes from the register.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K592000
component: BBa_J23100
component: BBa_B0034
sequence annotation: BBa_B0034
sequence annotation: BBa_J23100
sequence annotation: BBa_K592000
sequence: https://synbiohub.org/public/igem/BBa_K1660006_sequence/1

BBa_K592000
type: Component
persistent identity: component2446442
display id: component2446442
version: 1
top level: BBa_K1660006
access: public
definition: cph8

BBa_J23100
type: Component
persistent identity: component2446438
display id: component2446438
version: 1
top level: BBa_K1660006
access: public
definition: https://synbiohub.org/public/igem/BBa_J23100/1

BBa_B0034
type: Component
persistent identity: component2446440
display id: component2446440
version: 1
top level: BBa_K1660006
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2446440
display id: annotation2446440
version: 1
top level: BBa_K1660006
location: https://synbiohub.org/public/igem/BBa_K1660006/annotation2446440/range2446440/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1660006/annotation2446440/range2446440/1
type: Range
persistent identity: range2446440
display id: range2446440
version: 1
direction: direction/reverse
top level: BBa_K1660006
start: 44
end: 55
orientation: inline

BBa_J23100
type: SequenceAnnotation
persistent identity: annotation2446438
display id: annotation2446438
version: 1
top level: BBa_K1660006
location: https://synbiohub.org/public/igem/BBa_K1660006/annotation2446438/range2446438/1
component: BBa_J23100

https://synbiohub.org/public/igem/BBa_K1660006/annotation2446438/range2446438/1
type: Range
persistent identity: range2446438
display id: range2446438
version: 1
direction: direction/reverse
top level: BBa_K1660006
start: 1
end: 35
orientation: inline

BBa_K592000
type: SequenceAnnotation
persistent identity: annotation2446442
display id: annotation2446442
version: 1
top level: BBa_K1660006
location: https://synbiohub.org/public/igem/BBa_K1660006/annotation2446442/range2446442/1
component: BBa_K592000

https://synbiohub.org/public/igem/BBa_K1660006/annotation2446442/range2446442/1
type: Range
persistent identity: range2446442
display id: range2446442
version: 1
direction: direction/reverse
top level: BBa_K1660006
start: 62
end: 2299
orientation: inline

BBa_S05054
type: ComponentDefinition
persistent identity: BBa S05054
display id: BBa_S05054
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: K081024:I13507
creator: Muyuan Chen
created: 2012-09-17T11:00:00Z
modified: 2015-05-08T01:14:49Z
discontinued: false
dominant: false
group u list: _9_
m user id: 0
owner id: 12559
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_K081024
component: BBa_I13507
sequence annotation: BBa_K081024
sequence annotation: BBa_I13507
sequence: https://synbiohub.org/public/igem/BBa_S05054_sequence/1

BBa_K081024
type: Component
persistent identity: component2273946
display id: component2273946
version: 1
top level: BBa_S05054
access: public
definition: https://synbiohub.org/public/igem/BBa_K081024/1

BBa_I13507
type: Component
persistent identity: component2273958
display id: component2273958
version: 1
top level: BBa_S05054
access: public
definition: https://synbiohub.org/public/igem/BBa_I13507/1

BBa_K081024
type: SequenceAnnotation
persistent identity: annotation2273946
display id: annotation2273946
version: 1
top level: BBa_S05054
location: https://synbiohub.org/public/igem/BBa_S05054/annotation2273946/range2273946/1
component: BBa_K081024

https://synbiohub.org/public/igem/BBa_S05054/annotation2273946/range2273946/1
type: Range
persistent identity: range2273946
display id: range2273946
version: 1
direction: direction/reverse
top level: BBa_S05054
start: 1
end: 959
orientation: inline

BBa_I13507
type: SequenceAnnotation
persistent identity: annotation2273958
display id: annotation2273958
version: 1
top level: BBa_S05054
location: https://synbiohub.org/public/igem/BBa_S05054/annotation2273958/range2273958/1
component: BBa_I13507

https://synbiohub.org/public/igem/BBa_S05054/annotation2273958/range2273958/1
type: Range
persistent identity: range2273958
display id: range2273958
version: 1
direction: direction/reverse
top level: BBa_S05054
start: 968
end: 1828
orientation: inline

YtvA
type: ComponentDefinition
persistent identity: BBa K143037
display id: BBa_K143037
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: YtvA Blue Light Receptor for B.subtilis
creator: James Chappell
created: 2008-09-14T11:00:00Z
modified: 2015-05-08T01:10:24Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _199_
m user id: 0
owner id: 2090
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: YtvA is a blue light receptor endogenously found in ''B.subtilis''. Activation of the YtvA light receptor activates the mild stress response pathway, accumulating in sigma B activation. Previous research has shown that sigma B dependent transcription in response to blue light light exposure can be enhanced by the over expression of the YtvA receptor 1. We over expressed YtvA to enhance the transcriptional output of sigma B promoters in response to blue light.
mutable notes: Biobrick standard was applied to the YtvA sequence.
mutable provenance: The part was designed using the sequence from the B.subtilis genome. This sequence was then synthesised by Geneart.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: stop
sequence annotation: YtvA Blue Light Receptor for B.subtilis
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_K143037_sequence/1

stop
type: SequenceAnnotation
persistent identity: annotation1992715
display id: annotation1992715
version: 1
top level: YtvA
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K143037/annotation1992715/range1992715/1

https://synbiohub.org/public/igem/BBa_K143037/annotation1992715/range1992715/1
type: Range
persistent identity: range1992715
display id: range1992715
version: 1
direction: direction/forward
top level: YtvA
start: 787
end: 789
orientation: inline

YtvA Blue Light Receptor for B.subtilis
type: SequenceAnnotation
persistent identity: annotation1976412
display id: annotation1976412
version: 1
top level: YtvA
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K143037/annotation1976412/range1976412/1

https://synbiohub.org/public/igem/BBa_K143037/annotation1976412/range1976412/1
type: Range
persistent identity: range1976412
display id: range1976412
version: 1
direction: direction/forward
top level: YtvA
start: 1
end: 783
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation1992714
display id: annotation1992714
version: 1
top level: YtvA
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K143037/annotation1992714/range1992714/1

https://synbiohub.org/public/igem/BBa_K143037/annotation1992714/range1992714/1
type: Range
persistent identity: range1992714
display id: range1992714
version: 1
direction: direction/forward
top level: YtvA
start: 1
end: 3
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1992716
display id: annotation1992716
version: 1
top level: YtvA
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K143037/annotation1992716/range1992716/1

https://synbiohub.org/public/igem/BBa_K143037/annotation1992716/range1992716/1
type: Range
persistent identity: range1992716
display id: range1992716
version: 1
direction: direction/forward
top level: YtvA
start: 784
end: 786
orientation: inline

BBa_K881000
type: ComponentDefinition
persistent identity: BBa K881000
display id: BBa_K881000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: R-GECO1
creator: Nick van der Veeken
created: 2012-09-19T11:00:00Z
modified: 2015-05-08T01:13:40Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1144_
m user id: 0
owner id: 11729
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: Engineered red fluorescent protein that modulates its fluorescence in response to changes in calcium ion (Ca2+) concentration.
mutable notes: -
mutable provenance: Zhao et al. 2011, Science 30 September 2011: Vol. 333 no. 6051 pp. 1888-1891, DOI: 10.1126/science.1208592
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K881000_sequence/1

BBa_K990004
type: ComponentDefinition
persistent identity: BBa K990004
display id: BBa_K990004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Cph8(Cph1-EnvZ) system
creator: Fatma Betul CEVIK
created: 2013-06-20T11:00:00Z
modified: 2015-05-08T01:13:50Z
discontinued: false
dominant: false
group u list: _1287_
m user id: 0
owner id: 11713
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Cph8 synthesize under constitutive promoter.
mutable notes: sequence was checked NCBI
mutable provenance: Constitutive promoter, pTetR: R0040 Cph8: I15010
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_B0034
component: BBa_I15010
component: BBa_R0040
sequence annotation: BBa_R0040
sequence annotation: BBa_I15010
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K990004_sequence/1

BBa_B0034
type: Component
persistent identity: component2329963
display id: component2329963
version: 1
top level: BBa_K990004
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_I15010
type: Component
persistent identity: component2329966
display id: component2329966
version: 1
top level: BBa_K990004
access: public
definition: BBa_I15010

BBa_R0040
type: Component
persistent identity: component2329957
display id: component2329957
version: 1
top level: BBa_K990004
access: public
definition: https://synbiohub.org/public/igem/BBa_R0040/1

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2329957
display id: annotation2329957
version: 1
top level: BBa_K990004
location: https://synbiohub.org/public/igem/BBa_K990004/annotation2329957/range2329957/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_K990004/annotation2329957/range2329957/1
type: Range
persistent identity: range2329957
display id: range2329957
version: 1
direction: direction/forward
top level: BBa_K990004
start: 1
end: 54
orientation: inline

BBa_I15010
type: SequenceAnnotation
persistent identity: annotation2329966
display id: annotation2329966
version: 1
top level: BBa_K990004
location: https://synbiohub.org/public/igem/BBa_K990004/annotation2329966/range2329966/1
component: BBa_I15010

https://synbiohub.org/public/igem/BBa_K990004/annotation2329966/range2329966/1
type: Range
persistent identity: range2329966
display id: range2329966
version: 1
direction: direction/reverse
top level: BBa_K990004
start: 81
end: 2343
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2329963
display id: annotation2329963
version: 1
top level: BBa_K990004
location: https://synbiohub.org/public/igem/BBa_K990004/annotation2329963/range2329963/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K990004/annotation2329963/range2329963/1
type: Range
persistent identity: range2329963
display id: range2329963
version: 1
direction: direction/reverse
top level: BBa_K990004
start: 63
end: 74
orientation: inline