//function/sensor
type: Collection
persistent identity: function sensor
display id: function_sensor
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
owned by: james
owned by: myers
member: BBa_K1020002
member: BBa_K1946003
member: BBa_K1020007
member: BBa_K1170000
member: IscA1
member: BBa_K1682015
member: BBa_K1682002
member: BBa_K1315002
member: Ateam
member: BBa_K1020003
member: BY-Toggle
member: BBa_K1682012
member: IscA2
member: BBa_K1020012
member: BBa_K1682007
member: BBa_K1682008
member: BBa_K1682003
member: BBa_K862001
member: //function/sensor/lead
member: BBa_K1078001
member: BBa_K1497019
member: BBa_K1682011
member: BBa_K1497022
member: BBa_K1020004
member: //function/sensor/metal
member: BBa_K1682004
member: BBa_K862000
member: //function/sensor/light
member: BBa_K862002
member: pUSPA
member: BBa_K2056001
member: BBa_K1682009
member: BBa_K1682013
member: BBa_K1946004
member: BBa_K1497021
member: precB

//function/sensor/lead
type: Collection
persistent identity: function sensor lead
display id: function_sensor_lead
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
owned by: james
owned by: myers
member: https://synbiohub.org/public/igem/BBa_I721003/1
member: https://synbiohub.org/public/igem/BBa_I721004/1

//function/sensor/light
type: Collection
persistent identity: function sensor light
display id: function_sensor_light
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
owned by: james
owned by: myers
member: https://synbiohub.org/public/igem/BBa_K106010/1
member: https://synbiohub.org/public/igem/BBa_K1660004/1
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member: https://synbiohub.org/public/igem/BBa_I15009/1
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member: https://synbiohub.org/public/igem/BBa_K786001/1
member: https://synbiohub.org/public/igem/BBa_K1660007/1
member: https://synbiohub.org/public/igem/BBa_K559010/1
member: https://synbiohub.org/public/igem/BBa_K191009/1
member: https://synbiohub.org/public/igem/BBa_K786004/1
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member: https://synbiohub.org/public/igem/BBa_K1732028/1
member: https://synbiohub.org/public/igem/BBa_K559001/1
member: https://synbiohub.org/public/igem/BBa_V1012/1

//function/sensor/metal
type: Collection
persistent identity: function sensor metal
display id: function_sensor_metal
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
owned by: james
owned by: myers
member: https://synbiohub.org/public/igem/BBa_K1122069/1
member: https://synbiohub.org/public/igem/BBa_K1758354/1
member: https://synbiohub.org/public/igem/BBa_K346002/1
member: https://synbiohub.org/public/igem/BBa_K540001/1
member: https://synbiohub.org/public/igem/BBa_K824009/1
member: https://synbiohub.org/public/igem/BBa_K1342005/1
member: https://synbiohub.org/public/igem/BBa_K1758333/1
member: https://synbiohub.org/public/igem/BBa_J3902/1
member: https://synbiohub.org/public/igem/BBa_K896008/1
member: https://synbiohub.org/public/igem/BBa_K1755302/1
member: https://synbiohub.org/public/igem/BBa_K1980006/1
member: https://synbiohub.org/public/igem/BBa_K1980011/1
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member: https://synbiohub.org/public/igem/BBa_K1755305/1
member: https://synbiohub.org/public/igem/BBa_K1758331/1
member: https://synbiohub.org/public/igem/BBa_K1163110/1
member: https://synbiohub.org/public/igem/BBa_K1509001/1
member: https://synbiohub.org/public/igem/BBa_K1163107/1
member: https://synbiohub.org/public/igem/BBa_K1758342/1
member: https://synbiohub.org/public/igem/BBa_K1758313/1
member: https://synbiohub.org/public/igem/BBa_K1758312/1
member: https://synbiohub.org/public/igem/BBa_K1980004/1
member: https://synbiohub.org/public/igem/BBa_K1758325/1
member: https://synbiohub.org/public/igem/BBa_K824012/1
member: https://synbiohub.org/public/igem/BBa_I765000/1
member: https://synbiohub.org/public/igem/BBa_K1980005/1
member: https://synbiohub.org/public/igem/BBa_K1749000/1
member: https://synbiohub.org/public/igem/BBa_K1755303/1
member: https://synbiohub.org/public/igem/BBa_I721001/1
member: https://synbiohub.org/public/igem/BBa_K1980012/1
member: https://synbiohub.org/public/igem/BBa_K1758353/1
member: https://synbiohub.org/public/igem/BBa_I760005/1
member: https://synbiohub.org/public/igem/BBa_K1758332/1
member: https://synbiohub.org/public/igem/BBa_K174016/1
member: https://synbiohub.org/public/igem/BBa_K1758323/1
member: https://synbiohub.org/public/igem/BBa_I731004/1
member: https://synbiohub.org/public/igem/BBa_K1755301/1
member: https://synbiohub.org/public/igem/BBa_K1758322/1
member: https://synbiohub.org/public/igem/BBa_K1724000/1
member: https://synbiohub.org/public/igem/BBa_K1980007/1
member: https://synbiohub.org/public/igem/BBa_K1163101/1
member: https://synbiohub.org/public/igem/BBa_K346054/1
member: https://synbiohub.org/public/igem/BBa_K1758352/1
member: https://synbiohub.org/public/igem/BBa_K1758335/1
member: https://synbiohub.org/public/igem/BBa_K174017/1
member: https://synbiohub.org/public/igem/BBa_K1758334/1
member: https://synbiohub.org/public/igem/BBa_K1980008/1
member: https://synbiohub.org/public/igem/BBa_K1460009/1
member: https://synbiohub.org/public/igem/BBa_K824008/1
member: https://synbiohub.org/public/igem/BBa_K1758351/1
member: https://synbiohub.org/public/igem/BBa_K1980009/1
member: https://synbiohub.org/public/igem/BBa_K1758321/1

BBa_K1682008
type: ComponentDefinition
persistent identity: BBa K1682008
display id: BBa_K1682008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PkdpF[-15, T>C] - GFP generator
creator: iGEM15_HKUST-Rice
created: 2015-08-10T11:00:00Z
modified: 2015-09-18T09:09:50Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2100_
m user id: 8758
owner id: 21235
owning group id: 9
status: status/Planning
bookmark: false
mutable description: kdpFp[-15, T>A] - E0240
mutable notes: kdpFp[-15, T>A] - E0240
mutable provenance: kdpFp[-15, T>A] - E0240
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_K1682003
component: BBa_E0240
sequence annotation: BBa_K1682003
sequence annotation: BBa_E0240
sequence: https://synbiohub.org/public/igem/BBa_K1682008_sequence/1

BBa_K1682003
type: Component
persistent identity: component2434943
display id: component2434943
version: 1
top level: BBa_K1682008
access: public
definition: BBa_K1682003

BBa_E0240
type: Component
persistent identity: component2434954
display id: component2434954
version: 1
top level: BBa_K1682008
access: public
definition: https://synbiohub.org/public/igem/BBa_E0240/1

BBa_K1682003
type: SequenceAnnotation
persistent identity: annotation2434943
display id: annotation2434943
version: 1
top level: BBa_K1682008
location: https://synbiohub.org/public/igem/BBa_K1682008/annotation2434943/range2434943/1
component: BBa_K1682003

https://synbiohub.org/public/igem/BBa_K1682008/annotation2434943/range2434943/1
type: Range
persistent identity: range2434943
display id: range2434943
version: 1
direction: direction/reverse
top level: BBa_K1682008
start: 1
end: 78
orientation: inline

BBa_E0240
type: SequenceAnnotation
persistent identity: annotation2434954
display id: annotation2434954
version: 1
top level: BBa_K1682008
location: https://synbiohub.org/public/igem/BBa_K1682008/annotation2434954/range2434954/1
component: BBa_E0240

https://synbiohub.org/public/igem/BBa_K1682008/annotation2434954/range2434954/1
type: Range
persistent identity: range2434954
display id: range2434954
version: 1
direction: direction/reverse
top level: BBa_K1682008
start: 87
end: 962
orientation: inline

BBa_K1946003
type: ComponentDefinition
persistent identity: BBa K1946003
display id: BBa_K1946003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Cycloxehanone Sensor
creator: Musa Efe Işılak
created: 2016-10-18T11:00:00Z
modified: 2016-10-19T05:53:57Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2413_
m user id: 29843
owner id: 29843
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This part consists codon optimised ChnR (BBa_K1946000) from acinetobacter sp. operated by Tet operator and promoter region of ChnB (BBa_K1946001) followed by sfGFP (BBa_I746916). It is shown that ChnR activates ChnB protein in the presence of cyclohexanone. This sensor generates sfGFP in the presence of cyclohexanone.
mutable notes: ChnR gene synthesised with respect to Uniprot amino acid sequence. Codon optimised for E. Coli k12
mutable provenance: ChnR protein and pChnB promoter from acinetobacter sp.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
sequence annotation: B0010
sequence annotation: pL(tetO)
sequence annotation: BBa_K1946001
sequence annotation: rbs
sequence annotation: BBa_K1946000
sequence annotation: BBa_I746916
sequence: https://synbiohub.org/public/igem/BBa_K1946003_sequence/1

B0010
type: SequenceAnnotation
persistent identity: annotation2520783
display id: annotation2520783
version: 1
top level: BBa_K1946003
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1946003/annotation2520783/range2520783/1

https://synbiohub.org/public/igem/BBa_K1946003/annotation2520783/range2520783/1
type: Range
persistent identity: range2520783
display id: range2520783
version: 1
direction: direction/forward
top level: BBa_K1946003
start: 1021
end: 1125
orientation: inline

B0010
type: SequenceAnnotation
persistent identity: annotation2520787
display id: annotation2520787
version: 1
top level: BBa_K1946003
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1946003/annotation2520787/range2520787/1

https://synbiohub.org/public/igem/BBa_K1946003/annotation2520787/range2520787/1
type: Range
persistent identity: range2520787
display id: range2520787
version: 1
direction: direction/forward
top level: BBa_K1946003
start: 2425
end: 2529
orientation: inline

pL(tetO)
type: SequenceAnnotation
persistent identity: annotation2520780
display id: annotation2520780
version: 1
top level: BBa_K1946003
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1946003/annotation2520780/range2520780/1

https://synbiohub.org/public/igem/BBa_K1946003/annotation2520780/range2520780/1
type: Range
persistent identity: range2520780
display id: range2520780
version: 1
direction: direction/forward
top level: BBa_K1946003
start: 1
end: 56
orientation: inline

BBa_K1946001
type: SequenceAnnotation
persistent identity: annotation2520784
display id: annotation2520784
version: 1
top level: BBa_K1946003
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1946003/annotation2520784/range2520784/1

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type: Range
persistent identity: range2520784
display id: range2520784
version: 1
direction: direction/forward
top level: BBa_K1946003
start: 1132
end: 1668
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation2520781
display id: annotation2520781
version: 1
top level: BBa_K1946003
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1946003/annotation2520781/range2520781/1

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type: Range
persistent identity: range2520781
display id: range2520781
version: 1
direction: direction/forward
top level: BBa_K1946003
start: 57
end: 78
orientation: inline

BBa_K1946000
type: SequenceAnnotation
persistent identity: annotation2520782
display id: annotation2520782
version: 1
top level: BBa_K1946003
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1946003/annotation2520782/range2520782/1

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type: Range
persistent identity: range2520782
display id: range2520782
version: 1
direction: direction/forward
top level: BBa_K1946003
start: 79
end: 1020
orientation: inline

BBa_I746916
type: SequenceAnnotation
persistent identity: annotation2520786
display id: annotation2520786
version: 1
top level: BBa_K1946003
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1946003/annotation2520786/range2520786/1

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type: Range
persistent identity: range2520786
display id: range2520786
version: 1
direction: direction/forward
top level: BBa_K1946003
start: 1702
end: 2415
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation2520785
display id: annotation2520785
version: 1
top level: BBa_K1946003
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1946003/annotation2520785/range2520785/1

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type: Range
persistent identity: range2520785
display id: range2520785
version: 1
direction: direction/forward
top level: BBa_K1946003
start: 1675
end: 1689
orientation: inline

BBa_K1682004
type: ComponentDefinition
persistent identity: BBa K1682004
display id: BBa_K1682004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PkdpF[-15, T>G]
creator: iGEM15_HKUST-Rice
created: 2015-08-09T11:00:00Z
modified: 2015-09-18T09:16:31Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2100_
m user id: 8758
owner id: 21235
owning group id: 9
status: status/Planning
bookmark: false
mutable description: kdpFp[-15, T>G]
mutable notes: kdpFp[-15, T>G]
mutable provenance: kdpFp[-15, T>G]
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: phospho-KdpE binding site
sequence annotation: -35
sequence annotation: EcoRI Illegal site removed T->G
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_K1682004_sequence/1

phospho-KdpE binding site
type: SequenceAnnotation
persistent identity: annotation2473581
display id: annotation2473581
version: 1
top level: BBa_K1682004
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1682004/annotation2473581/range2473581/1

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type: Range
persistent identity: range2473581
display id: range2473581
version: 1
direction: direction/forward
top level: BBa_K1682004
start: 8
end: 24
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2473584
display id: annotation2473584
version: 1
top level: BBa_K1682004
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1682004/annotation2473584/range2473584/1

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type: Range
persistent identity: range2473584
display id: range2473584
version: 1
direction: direction/forward
top level: BBa_K1682004
start: 42
end: 47
orientation: inline

EcoRI Illegal site removed T->G
type: SequenceAnnotation
persistent identity: annotation2475215
display id: annotation2475215
version: 1
top level: BBa_K1682004
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K1682004/annotation2475215/range2475215/1

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type: Range
persistent identity: range2475215
display id: range2475215
version: 1
direction: direction/forward
top level: BBa_K1682004
start: 65
end: 66
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2473582
display id: annotation2473582
version: 1
top level: BBa_K1682004
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1682004/annotation2473582/range2473582/1

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type: Range
persistent identity: range2473582
display id: range2473582
version: 1
direction: direction/forward
top level: BBa_K1682004
start: 69
end: 74
orientation: inline

IscA2
type: ComponentDefinition
persistent identity: BBa M36908
display id: BBa_M36908
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Coding Sequence of IscA2 pigeon homologue (codon-optimized for humans)
creator: Michael Becich
created: 2015-10-24T11:00:00Z
modified: 2015-12-04T08:06:13Z
discontinued: false
dominant: false
experience: experience/Fails
group u list: _848_
m user id: 29123
owner id: 29123
owning group id: 9
status: status/Planning
bookmark: false
mutable description: The gene Iron-Sulfur cluster assembly 2 (IscA2) is a mitochondrial protein involved in the biogenesis and assembly of iron-sulfur clusters, which play a role in electron-transfer reactions and is theorized to be associated with certain organisms' magnetic perception. The homing pigeon is one such organism with this ability, and here we have codon-optimized the Columba Livia homologue for homo sapiens. The sequence can be inserted into plasmids for expression of the magnetoreceptor IscA gene, which hopefully will create magnetogenetic biosensors in the form of iron-sulfur cluster, for downstream output and applications.
mutable notes: While the plasmid design for this insert can be complicated, the coding sequence itself is quite simple. We only needed to add the start codon since this second isoform did not have methionine built in.
mutable provenance: The predicted IscA amino acid sequence was obtained from NCBI (http://www.ncbi.nlm.nih.gov/protein/XP_005502393.1), and codon-optimized for humans with IDT's tools.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316

BBa_K1497019
type: ComponentDefinition
persistent identity: BBa K1497019
display id: BBa_K1497019
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: fdeR (Naringenin binding protein) and fde operon regulatory domain
creator: Sascha Hein, Niklas Hummel, Sebastian Barthel, Thomas Dohmen
created: 2014-10-06T11:00:00Z
modified: 2015-05-08T01:10:45Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1877_
m user id: 0
owner id: 12466
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: fdeR - Naringenin binding protein We modified the naringenin biosensor from Siedler et al. by elimination of one EcoRI restriction site and by changing the native ribosomal binding site into the strong RBS Bba_B0034. Based on this reporter protein and 3 different fluorescence proteins, we designed 3 new biosensors for in vivo detection and determination of naringenin. The natural source of FdeR is Herbaspirillum seropedicae. You can use our reporter for measuring naringenin concentrations in your samples. Depending on which fluorophor you want to detect, you can use one of three biosensors: GFP K1497020 mKate (DsRed variant) K1497021 CFP K1497022 You can create your own naringenin sensor or your own naringenin dependent gene expression device as well. For these reasons use FdeR Biobrick K1497019 and clone your parts of interest (without RBS!) behind the device.
mutable notes: d
mutable provenance: d
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Signalling
role: SO:0000804
sequence: https://synbiohub.org/public/igem/BBa_K1497019_sequence/1

BBa_K1020007
type: ComponentDefinition
persistent identity: BBa K1020007
display id: BBa_K1020007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: AlkR -PSB1C3-High constitutive
creator: Yue Liu
created: 2013-09-09T11:00:00Z
modified: 2015-05-08T01:08:43Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1327_
m user id: 0
owner id: 16881
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: AlkR is a transcriptional regulator belonging to AraC/Xyls family, which could detect a broad range of alkanes and alkenes with carbon chain length from C7 to C36. It is said that AlkR is the only bioreporter that is able to detect alkane with carbon chain length greater than C18.
mutable notes: AlkR with strong promoter and terminator.
mutable provenance: Acinetobacter baylyi ADP1
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_J23100
component: BBa_K1020002
component: BBa_B0034
component: BBa_B0015
sequence annotation: BBa_B0034
sequence annotation: BBa_K1020002
sequence annotation: BBa_B0015
sequence annotation: BBa_J23100
sequence: https://synbiohub.org/public/igem/BBa_K1020007_sequence/1

BBa_J23100
type: Component
persistent identity: component2338165
display id: component2338165
version: 1
top level: BBa_K1020007
access: public
definition: https://synbiohub.org/public/igem/BBa_J23100/1

BBa_K1020002
type: Component
persistent identity: component2338169
display id: component2338169
version: 1
top level: BBa_K1020007
access: public
definition: BBa_K1020002

BBa_B0034
type: Component
persistent identity: component2338167
display id: component2338167
version: 1
top level: BBa_K1020007
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0015
type: Component
persistent identity: component2338176
display id: component2338176
version: 1
top level: BBa_K1020007
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2338167
display id: annotation2338167
version: 1
top level: BBa_K1020007
location: https://synbiohub.org/public/igem/BBa_K1020007/annotation2338167/range2338167/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1020007/annotation2338167/range2338167/1
type: Range
persistent identity: range2338167
display id: range2338167
version: 1
direction: direction/reverse
top level: BBa_K1020007
start: 44
end: 55
orientation: inline

BBa_K1020002
type: SequenceAnnotation
persistent identity: annotation2338169
display id: annotation2338169
version: 1
top level: BBa_K1020007
location: https://synbiohub.org/public/igem/BBa_K1020007/annotation2338169/range2338169/1
component: BBa_K1020002

https://synbiohub.org/public/igem/BBa_K1020007/annotation2338169/range2338169/1
type: Range
persistent identity: range2338169
display id: range2338169
version: 1
direction: direction/reverse
top level: BBa_K1020007
start: 62
end: 988
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2338176
display id: annotation2338176
version: 1
top level: BBa_K1020007
location: https://synbiohub.org/public/igem/BBa_K1020007/annotation2338176/range2338176/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K1020007/annotation2338176/range2338176/1
type: Range
persistent identity: range2338176
display id: range2338176
version: 1
direction: direction/reverse
top level: BBa_K1020007
start: 997
end: 1125
orientation: inline

BBa_J23100
type: SequenceAnnotation
persistent identity: annotation2338165
display id: annotation2338165
version: 1
top level: BBa_K1020007
location: https://synbiohub.org/public/igem/BBa_K1020007/annotation2338165/range2338165/1
component: BBa_J23100

https://synbiohub.org/public/igem/BBa_K1020007/annotation2338165/range2338165/1
type: Range
persistent identity: range2338165
display id: range2338165
version: 1
direction: direction/reverse
top level: BBa_K1020007
start: 1
end: 35
orientation: inline

BBa_K1682007
type: ComponentDefinition
persistent identity: BBa K1682007
display id: BBa_K1682007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PkdpF[-15, T>A] - GFP generator
creator: iGEM15_HKUST-Rice
created: 2015-08-10T11:00:00Z
modified: 2015-09-18T09:10:21Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2100_
m user id: 8758
owner id: 21235
owning group id: 9
status: status/Planning
bookmark: false
mutable description: kdpFp[-15, T>A]
mutable notes: kdpFp[-15, T>A]
mutable provenance: kdpFp[-15, T>A]
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_K1682002
component: BBa_E0240
sequence annotation: BBa_E0240
sequence annotation: BBa_K1682002
sequence: https://synbiohub.org/public/igem/BBa_K1682007_sequence/1

BBa_K1682002
type: Component
persistent identity: component2434917
display id: component2434917
version: 1
top level: BBa_K1682007
access: public
definition: BBa_K1682002

BBa_E0240
type: Component
persistent identity: component2434928
display id: component2434928
version: 1
top level: BBa_K1682007
access: public
definition: https://synbiohub.org/public/igem/BBa_E0240/1

BBa_E0240
type: SequenceAnnotation
persistent identity: annotation2434928
display id: annotation2434928
version: 1
top level: BBa_K1682007
location: https://synbiohub.org/public/igem/BBa_K1682007/annotation2434928/range2434928/1
component: BBa_E0240

https://synbiohub.org/public/igem/BBa_K1682007/annotation2434928/range2434928/1
type: Range
persistent identity: range2434928
display id: range2434928
version: 1
direction: direction/reverse
top level: BBa_K1682007
start: 87
end: 962
orientation: inline

BBa_K1682002
type: SequenceAnnotation
persistent identity: annotation2434917
display id: annotation2434917
version: 1
top level: BBa_K1682007
location: https://synbiohub.org/public/igem/BBa_K1682007/annotation2434917/range2434917/1
component: BBa_K1682002

https://synbiohub.org/public/igem/BBa_K1682007/annotation2434917/range2434917/1
type: Range
persistent identity: range2434917
display id: range2434917
version: 1
direction: direction/reverse
top level: BBa_K1682007
start: 1
end: 78
orientation: inline

BBa_K862002
type: ComponentDefinition
persistent identity: BBa K862002
display id: BBa_K862002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: precB-LacZ-doubleTerminator
creator: Charlotte Bunne, Mariam Harmouche, Stefan Holderbach, Anna Huhn and Jakob Kreft (in alphabetical order)
created: 2012-06-07T11:00:00Z
modified: 2015-05-08T01:13:36Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1122_
m user id: 0
owner id: 3317
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This is a part for the precise quantification of UV-radiation or radioactive radiation in E. coli (recA+) strains, i.e. BL21(DE3). It consits of a recB Promoter (part BBa_K862003) fused to a LacZ reporter cloned in front of a double terminator (part BBa_K173004).
mutable notes: The part was first constructed in plasmid backbone pSB1AK3 and then subcloned into pSB1C3 for submission to the registry.
mutable provenance: The part was constructed by standard cloning from parts BBa_K862003 (precB) and BBa_K173004 (LacZ).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_K862003
component: BBa_K173004
sequence annotation: BBa_K862003
sequence annotation: BBa_K173004
sequence: https://synbiohub.org/public/igem/BBa_K862002_sequence/1

BBa_K862003
type: Component
persistent identity: component2176180
display id: component2176180
version: 1
top level: BBa_K862002
access: public
definition: precB

BBa_K173004
type: Component
persistent identity: component2176194
display id: component2176194
version: 1
top level: BBa_K862002
access: public
definition: https://synbiohub.org/public/igem/BBa_K173004/1

BBa_K862003
type: SequenceAnnotation
persistent identity: annotation2176180
display id: annotation2176180
version: 1
top level: BBa_K862002
location: https://synbiohub.org/public/igem/BBa_K862002/annotation2176180/range2176180/1
component: BBa_K862003

https://synbiohub.org/public/igem/BBa_K862002/annotation2176180/range2176180/1
type: Range
persistent identity: range2176180
display id: range2176180
version: 1
direction: direction/reverse
top level: BBa_K862002
start: 1
end: 70
orientation: inline

BBa_K173004
type: SequenceAnnotation
persistent identity: annotation2176194
display id: annotation2176194
version: 1
top level: BBa_K862002
location: https://synbiohub.org/public/igem/BBa_K862002/annotation2176194/range2176194/1
component: BBa_K173004

https://synbiohub.org/public/igem/BBa_K862002/annotation2176194/range2176194/1
type: Range
persistent identity: range2176194
display id: range2176194
version: 1
direction: direction/reverse
top level: BBa_K862002
start: 79
end: 3308
orientation: inline

pUSPA
type: ComponentDefinition
persistent identity: BBa K1620000
display id: BBa_K1620000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter element UspA
creator: Celio Dias Santos Jr
created: 2015-09-02T11:00:00Z
modified: 2015-09-05T12:59:35Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2037_
m user id: 23597
owner id: 23597
owning group id: 9
status: status/Planning
bookmark: true
mutable description: The universal stress protein A is a response of Escherichia coli cells to growth arrest, and its lacking generates cells with defective growth.This genetic element is dependent to sigma-70 factor. Its regulation is done through the concentration of a specific stationary phase allormone, guanosine-5'-diphosphate-3'-diphosphate (ppGp), as described elsewhere. The ppGp activate the transcription of downstream elements through a positive regulation of the β-subunit of RNA polymerase. In this sense, the PuspA element is considered a stationary phase promoter. However, its behavior is like a constitutive transcription promotion, becoming higher in stress situations. Diverse conditions make the Escherichia coli cell enter to stress state, like heat shocks, starvation, osmotic stress, ultraviolet light and other conditions. In these situations, the RNA polimerase sigma factors (ropS) trigger the expression of chaperones and other stress protector molecules, in order to help the cell survive. In our project we have used this promoter to trigger the expression of limonene synthase after an osmotic shock. Other uses of this part includes the environmental stress detectors making using a reporter system coupled to this promoter.
mutable notes: The original promoter site was shortened and the last three nucleotides were changed to restriction site adjustment.
mutable provenance: We have drawn the sequence for an improved PuspA promoter from an analysis of 400bp upstream the Universal Protein A (UspA) gene in E. coli K12 MG16555 genome. After design, the DNA template of part was synthesized by IDT and was amplified from a G-block and assembled using 3A assembly method.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: UspA
sequence: https://synbiohub.org/public/igem/BBa_K1620000_sequence/1

UspA
type: SequenceAnnotation
persistent identity: annotation2443284
display id: annotation2443284
version: 1
top level: pUSPA
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1620000/annotation2443284/range2443284/1

https://synbiohub.org/public/igem/BBa_K1620000/annotation2443284/range2443284/1
type: Range
persistent identity: range2443284
display id: range2443284
version: 1
direction: direction/forward
top level: pUSPA
start: 1
end: 46
orientation: inline

BBa_K1078001
type: ComponentDefinition
persistent identity: BBa K1078001
display id: BBa_K1078001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Strong reporter device optimized for Pichia pastoris, activated by methanol
creator: Edgar Andres Ochoa Cruz
created: 2013-09-11T11:00:00Z
modified: 2015-05-08T01:09:03Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1387_
m user id: 0
owner id: 11298
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: This device is composed by three parts. The first part is a modified promoter pAOX, it was found in a library where it showed more than 60% stronger activation when compared with the wild-type, as reported by Hartner FS, Ruth C, Langenegger D, Johnson SN, Hyka P, Lin-Cereghino GP, Lin-Cereghino J, Kovar K, Cregg JM, Glieder A: Promoter library designed for fine-tuned gene expression in Pichia pastoris. Nucleic Acids Res 2008, 36:e76. The second part is the kozak sequence for Pichia pastoris, and the third is a Red fluorescent protein condon optimized for expression in Pichia pastoris. This device was created to express a reporter gene in the presence of ethanol, and is inactivated by ethanol, when combined with the modified Mxr1 (BBa_K1078000), it is not inactivated by ethanol. This is useful for our biosensor design, which aims to detect levels of methanol above 2% in common alcoholic drinks (normal content of 10 to 60% ethanol). This will allow government to make high-throughput screening of ethanol drinks tainted will methanol. The modified Mxr1 is able to activate the pAOX promoter, in ethanol, glycerol and methanol. This is useful for our biosensor design, which aims to detect levels of methanol above 2% in common alcoholic drinks (normal content of 10 to 60% ethanol). This will allow government to make high-throughput screening of ethanol drinks tainted will methanol.
mutable notes: After synthesis, in order to build our sensor the part was introduced in pPIC9K plasmid to allow its genomic recombination into the Pichia pastoris genome, substituting the original Mxr1 found in the yeast. This part does not have a single biobrick version with the Mx1p because both must be inserted in different genome locations at the Pichia pastoris
mutable provenance: he part was obtained by synthesis, the modified Mxr1p sequence was found in Hartner FS, Ruth C, Langenegger D, Johnson SN, Hyka P, Lin-Cereghino GP, Lin-Cereghino J, Kovar K, Cregg JM, Glieder A: Promoter library designed for fine-tuned gene expression in Pichia pastoris. Nucleic Acids Res 2008, 36:e76
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
sequence annotation: pAOX modified
sequence annotation: Kozak sequence
sequence annotation: RFP modified
sequence: https://synbiohub.org/public/igem/BBa_K1078001_sequence/1

pAOX modified
type: SequenceAnnotation
persistent identity: annotation2339065
display id: annotation2339065
version: 1
top level: BBa_K1078001
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1078001/annotation2339065/range2339065/1

https://synbiohub.org/public/igem/BBa_K1078001/annotation2339065/range2339065/1
type: Range
persistent identity: range2339065
display id: range2339065
version: 1
direction: direction/forward
top level: BBa_K1078001
start: 1
end: 887
orientation: inline

Kozak sequence
type: SequenceAnnotation
persistent identity: annotation2339071
display id: annotation2339071
version: 1
top level: BBa_K1078001
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1078001/annotation2339071/range2339071/1

https://synbiohub.org/public/igem/BBa_K1078001/annotation2339071/range2339071/1
type: Range
persistent identity: range2339071
display id: range2339071
version: 1
direction: direction/forward
top level: BBa_K1078001
start: 888
end: 899
orientation: inline

RFP modified
type: SequenceAnnotation
persistent identity: annotation2339072
display id: annotation2339072
version: 1
top level: BBa_K1078001
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1078001/annotation2339072/range2339072/1

https://synbiohub.org/public/igem/BBa_K1078001/annotation2339072/range2339072/1
type: Range
persistent identity: range2339072
display id: range2339072
version: 1
direction: direction/forward
top level: BBa_K1078001
start: 900
end: 1577
orientation: inline

BBa_K1682015
type: ComponentDefinition
persistent identity: BBa K1682015
display id: BBa_K1682015
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PphoBR-I13504
creator: iGEM15_HKUST-Rice
created: 2015-09-09T11:00:00Z
modified: 2015-09-18T10:30:52Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2100_
m user id: 16893
owner id: 26069
owning group id: 9
status: status/Planning
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
sequence annotation: phospho-phoB Bindind site
sequence: https://synbiohub.org/public/igem/BBa_K1682015_sequence/1

phospho-phoB Bindind site
type: SequenceAnnotation
persistent identity: annotation2475990
display id: annotation2475990
version: 1
top level: BBa_K1682015
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1682015/annotation2475990/range2475990/1

https://synbiohub.org/public/igem/BBa_K1682015/annotation2475990/range2475990/1
type: Range
persistent identity: range2475990
display id: range2475990
version: 1
direction: direction/forward
top level: BBa_K1682015
start: 21
end: 39
orientation: inline

Ateam
type: ComponentDefinition
persistent identity: BBa K1023016
display id: BBa_K1023016
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: FRET based ATP biosensor for expression in Aspergillus niger
creator: Shreyans Chordia
created: 2013-09-26T11:00:00Z
modified: 2015-05-08T01:08:45Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1330_
m user id: 0
owner id: 15671
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Consists of cfp-yfp fret pair that responds to changes in ATP concentration
mutable notes: It has illegal restriction sites for the standard 10 assembly. The cds of the part is flanked by NdeI and NotI restriction sites.
mutable provenance: Imamura lab
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
sequence annotation: BBa_K1023002
sequence annotation: BBa_K1023001
sequence: https://synbiohub.org/public/igem/BBa_K1023016_sequence/1

BBa_K1023002
type: SequenceAnnotation
persistent identity: annotation2364482
display id: annotation2364482
version: 1
top level: Ateam
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1023016/annotation2364482/range2364482/1

https://synbiohub.org/public/igem/BBa_K1023016/annotation2364482/range2364482/1
type: Range
persistent identity: range2364482
display id: range2364482
version: 1
direction: direction/forward
top level: Ateam
start: 711
end: 1546
orientation: inline

BBa_K1023001
type: SequenceAnnotation
persistent identity: annotation2364481
display id: annotation2364481
version: 1
top level: Ateam
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1023016/annotation2364481/range2364481/1

https://synbiohub.org/public/igem/BBa_K1023016/annotation2364481/range2364481/1
type: Range
persistent identity: range2364481
display id: range2364481
version: 1
direction: direction/forward
top level: Ateam
start: 1
end: 702
orientation: inline

BBa_K2056001
type: ComponentDefinition
persistent identity: BBa K2056001
display id: BBa_K2056001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: NsrR - NO(x) sensitive pYear, pNirk repressor protein
creator: Muhammad Ismail
created: 2016-10-12T11:00:00Z
modified: 2016-10-13T10:04:47Z
discontinued: false
dominant: false
group u list: _2524_
m user id: 33019
owner id: 33019
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This part contains the NsrR (NO(x) sensitive pYear, pNirk repressor protein) encoding region. Escherichia coli (E. coli) detects environmental nitrate by the yeaR-yoaG operon. PyeaR is regulated by the NsrR repressor protein. Nitric oxide binds to the NsrR protein and relieves the repression on PyeaR. As a result, any genes that are downstream of PyeaR are expressed. This part is also related with BBa_K2056002, the promoter region of the NirK protein, because NsrR activates the transcription of NirK under high nitrite conditions. Due to these properties, we can use this part as a sensor for oxides of nitrogen.
mutable notes: Because the part Bba_K1682011 was not available from the registry, we had to get it synthesized. We had to codon optimized this part to conform to assembly standards and the requirements for synthesis.
mutable provenance: This part has been designed after the part Bba_K1682011 in the Registry of Standard Biological Parts.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K2056001_sequence/1

BBa_K1682013
type: ComponentDefinition
persistent identity: BBa K1682013
display id: BBa_K1682013
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PphoA - I13504
creator: iGEM15_HKUST-Rice
created: 2015-09-01T11:00:00Z
modified: 2015-09-18T09:17:22Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2100_
m user id: 8758
owner id: 26069
owning group id: 9
status: status/Planning
bookmark: false
mutable description: phoApis a phosphate-responsive promoter. In high concentration of phosphate, it will be repressed while in low phosphate concentration, it will be activated.
mutable notes: N/A
mutable provenance: phoAp is native in E. coli
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_K1682012
component: BBa_I13504
sequence annotation: BBa_I13504
sequence annotation: BBa_K1682012
sequence: https://synbiohub.org/public/igem/BBa_K1682013_sequence/1

BBa_K1682012
type: Component
persistent identity: component2473570
display id: component2473570
version: 1
top level: BBa_K1682013
access: public
definition: BBa_K1682012

BBa_I13504
type: Component
persistent identity: component2473580
display id: component2473580
version: 1
top level: BBa_K1682013
access: public
definition: https://synbiohub.org/public/igem/BBa_I13504/1

BBa_I13504
type: SequenceAnnotation
persistent identity: annotation2473580
display id: annotation2473580
version: 1
top level: BBa_K1682013
location: https://synbiohub.org/public/igem/BBa_K1682013/annotation2473580/range2473580/1
component: BBa_I13504

https://synbiohub.org/public/igem/BBa_K1682013/annotation2473580/range2473580/1
type: Range
persistent identity: range2473580
display id: range2473580
version: 1
direction: direction/reverse
top level: BBa_K1682013
start: 94
end: 968
orientation: inline

BBa_K1682012
type: SequenceAnnotation
persistent identity: annotation2473570
display id: annotation2473570
version: 1
top level: BBa_K1682013
location: https://synbiohub.org/public/igem/BBa_K1682013/annotation2473570/range2473570/1
component: BBa_K1682012

https://synbiohub.org/public/igem/BBa_K1682013/annotation2473570/range2473570/1
type: Range
persistent identity: range2473570
display id: range2473570
version: 1
direction: direction/reverse
top level: BBa_K1682013
start: 1
end: 85
orientation: inline

BBa_K1946004
type: ComponentDefinition
persistent identity: BBa K1946004
display id: BBa_K1946004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Toluene/Xylene Sensor
creator: Ayşe Naz Ozant??rk
created: 2016-10-18T11:00:00Z
modified: 2016-10-19T10:23:29Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2413_
m user id: 29837
owner id: 29837
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This part responds to the presence of toluene, m-, p- or o-xylenes and produces a green fluorescent signal. The transcriptional regulatory protein XylR recognizes these organic molecules and activates pu promoter which in turn will lead to the synthesis of sfGFP.
mutable notes: XylR (BBa_K1946005) in this composite part was codon optimized by us for E.coli K12.
mutable provenance: The source of XylR (BBa_K1946005) can be found at GenBank: AAB59162.1 . pu promoter (BBa_K1946006) was synthetically synthesized from GeneWiz based on Pseudomonas putida TOL plasmid. sfGFP sequence can be accessed in the registry at BBa_I746916.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
sequence annotation: RBS
sequence annotation: rrnB T1 (B0010)
sequence annotation: pL(tetO)
sequence annotation: pu (K1946005)
sequence annotation: sfGFP (I746916)
sequence annotation: XylR (K1946005)
sequence: https://synbiohub.org/public/igem/BBa_K1946004_sequence/1

RBS
type: SequenceAnnotation
persistent identity: annotation2521528
display id: annotation2521528
version: 1
top level: BBa_K1946004
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1946004/annotation2521528/range2521528/1

https://synbiohub.org/public/igem/BBa_K1946004/annotation2521528/range2521528/1
type: Range
persistent identity: range2521528
display id: range2521528
version: 1
direction: direction/forward
top level: BBa_K1946004
start: 2227
end: 2241
orientation: inline

rrnB T1 (B0010)
type: SequenceAnnotation
persistent identity: annotation2521526
display id: annotation2521526
version: 1
top level: BBa_K1946004
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1946004/annotation2521526/range2521526/1

https://synbiohub.org/public/igem/BBa_K1946004/annotation2521526/range2521526/1
type: Range
persistent identity: range2521526
display id: range2521526
version: 1
direction: direction/forward
top level: BBa_K1946004
start: 1788
end: 1892
orientation: inline

pL(tetO)
type: SequenceAnnotation
persistent identity: annotation2521523
display id: annotation2521523
version: 1
top level: BBa_K1946004
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1946004/annotation2521523/range2521523/1

https://synbiohub.org/public/igem/BBa_K1946004/annotation2521523/range2521523/1
type: Range
persistent identity: range2521523
display id: range2521523
version: 1
direction: direction/forward
top level: BBa_K1946004
start: 3
end: 57
orientation: inline

pu (K1946005)
type: SequenceAnnotation
persistent identity: annotation2521527
display id: annotation2521527
version: 1
top level: BBa_K1946004
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1946004/annotation2521527/range2521527/1

https://synbiohub.org/public/igem/BBa_K1946004/annotation2521527/range2521527/1
type: Range
persistent identity: range2521527
display id: range2521527
version: 1
direction: direction/forward
top level: BBa_K1946004
start: 1899
end: 2220
orientation: inline

rrnB T1 (B0010)
type: SequenceAnnotation
persistent identity: annotation2521530
display id: annotation2521530
version: 1
top level: BBa_K1946004
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1946004/annotation2521530/range2521530/1

https://synbiohub.org/public/igem/BBa_K1946004/annotation2521530/range2521530/1
type: Range
persistent identity: range2521530
display id: range2521530
version: 1
direction: direction/forward
top level: BBa_K1946004
start: 2977
end: 3081
orientation: inline

sfGFP (I746916)
type: SequenceAnnotation
persistent identity: annotation2521529
display id: annotation2521529
version: 1
top level: BBa_K1946004
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1946004/annotation2521529/range2521529/1

https://synbiohub.org/public/igem/BBa_K1946004/annotation2521529/range2521529/1
type: Range
persistent identity: range2521529
display id: range2521529
version: 1
direction: direction/forward
top level: BBa_K1946004
start: 2254
end: 2967
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2521524
display id: annotation2521524
version: 1
top level: BBa_K1946004
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1946004/annotation2521524/range2521524/1

https://synbiohub.org/public/igem/BBa_K1946004/annotation2521524/range2521524/1
type: Range
persistent identity: range2521524
display id: range2521524
version: 1
direction: direction/forward
top level: BBa_K1946004
start: 73
end: 77
orientation: inline

XylR (K1946005)
type: SequenceAnnotation
persistent identity: annotation2521525
display id: annotation2521525
version: 1
top level: BBa_K1946004
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1946004/annotation2521525/range2521525/1

https://synbiohub.org/public/igem/BBa_K1946004/annotation2521525/range2521525/1
type: Range
persistent identity: range2521525
display id: range2521525
version: 1
direction: direction/forward
top level: BBa_K1946004
start: 87
end: 1784
orientation: inline

BBa_K1020003
type: ComponentDefinition
persistent identity: BBa K1020003
display id: BBa_K1020003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: AlkR -PSB1C3-Low constitutive
creator: Yue Liu
created: 2013-08-17T11:00:00Z
modified: 2015-05-08T01:08:43Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1327_
m user id: 0
owner id: 16881
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: AlkR is a transcriptional regulator belonging to AraC/Xyls family, which could detect a broad range of alkanes and alkenes with carbon chain length from C7 to C36. It is said that AlkR is the only bioreporter that is able to detect alkane with carbon chain length greater than C18. The mechanism is that alkanes are presumably recognized by AlkR regulatory protein and their interaction triggered a conformation change of AlkR dimers which isomerizes the promoter-RNAP complex and lead to the activation of alkM gene. AlkR regulatory protein includes C-terminal DNA-binding domain(CTD) for promoter binding and N-terminal domain(NTD) for inducer recognition. Here we use a constitutive promoter for AlkR gene and in case of interference, we choose a long and strong terminator.
mutable notes: We use a constitutive promoter for AlkR gene and we choose a long and strong terminator to prevent interference.
mutable provenance: Acinetobacter baylyi ADP1
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_J23103
component: BBa_K1020002
component: BBa_B0034
component: BBa_B0015
sequence annotation: BBa_B0015
sequence annotation: BBa_B0034
sequence annotation: BBa_J23103
sequence annotation: BBa_K1020002
sequence: https://synbiohub.org/public/igem/BBa_K1020003_sequence/1

BBa_J23103
type: Component
persistent identity: component2333537
display id: component2333537
version: 1
top level: BBa_K1020003
access: public
definition: https://synbiohub.org/public/igem/BBa_J23103/1

BBa_K1020002
type: Component
persistent identity: component2333541
display id: component2333541
version: 1
top level: BBa_K1020003
access: public
definition: BBa_K1020002

BBa_B0034
type: Component
persistent identity: component2333539
display id: component2333539
version: 1
top level: BBa_K1020003
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0015
type: Component
persistent identity: component2333548
display id: component2333548
version: 1
top level: BBa_K1020003
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2333548
display id: annotation2333548
version: 1
top level: BBa_K1020003
location: https://synbiohub.org/public/igem/BBa_K1020003/annotation2333548/range2333548/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K1020003/annotation2333548/range2333548/1
type: Range
persistent identity: range2333548
display id: range2333548
version: 1
direction: direction/reverse
top level: BBa_K1020003
start: 997
end: 1125
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2333539
display id: annotation2333539
version: 1
top level: BBa_K1020003
location: https://synbiohub.org/public/igem/BBa_K1020003/annotation2333539/range2333539/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1020003/annotation2333539/range2333539/1
type: Range
persistent identity: range2333539
display id: range2333539
version: 1
direction: direction/reverse
top level: BBa_K1020003
start: 44
end: 55
orientation: inline

BBa_J23103
type: SequenceAnnotation
persistent identity: annotation2333537
display id: annotation2333537
version: 1
top level: BBa_K1020003
location: https://synbiohub.org/public/igem/BBa_K1020003/annotation2333537/range2333537/1
component: BBa_J23103

https://synbiohub.org/public/igem/BBa_K1020003/annotation2333537/range2333537/1
type: Range
persistent identity: range2333537
display id: range2333537
version: 1
direction: direction/reverse
top level: BBa_K1020003
start: 1
end: 35
orientation: inline

BBa_K1020002
type: SequenceAnnotation
persistent identity: annotation2333541
display id: annotation2333541
version: 1
top level: BBa_K1020003
location: https://synbiohub.org/public/igem/BBa_K1020003/annotation2333541/range2333541/1
component: BBa_K1020002

https://synbiohub.org/public/igem/BBa_K1020003/annotation2333541/range2333541/1
type: Range
persistent identity: range2333541
display id: range2333541
version: 1
direction: direction/reverse
top level: BBa_K1020003
start: 62
end: 988
orientation: inline

BBa_K1682003
type: ComponentDefinition
persistent identity: BBa K1682003
display id: BBa_K1682003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PkdpF[-15, T>C]
creator: iGEM15_HKUST-Rice
created: 2015-08-09T11:00:00Z
modified: 2015-09-18T09:16:08Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2100_
m user id: 8758
owner id: 21235
owning group id: 9
status: status/Planning
bookmark: false
mutable description: kdpFp[-15, T>C]
mutable notes: kdpFp[-15, T>C]
mutable provenance: kdpFp[-15, T>C]
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: EcoRI Illegal site removed T->C
sequence annotation: -10
sequence annotation: phospho-KdpE binding site
sequence annotation: -35
sequence: https://synbiohub.org/public/igem/BBa_K1682003_sequence/1

EcoRI Illegal site removed T->C
type: SequenceAnnotation
persistent identity: annotation2475214
display id: annotation2475214
version: 1
top level: BBa_K1682003
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K1682003/annotation2475214/range2475214/1

https://synbiohub.org/public/igem/BBa_K1682003/annotation2475214/range2475214/1
type: Range
persistent identity: range2475214
display id: range2475214
version: 1
direction: direction/forward
top level: BBa_K1682003
start: 65
end: 66
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2473589
display id: annotation2473589
version: 1
top level: BBa_K1682003
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1682003/annotation2473589/range2473589/1

https://synbiohub.org/public/igem/BBa_K1682003/annotation2473589/range2473589/1
type: Range
persistent identity: range2473589
display id: range2473589
version: 1
direction: direction/forward
top level: BBa_K1682003
start: 69
end: 74
orientation: inline

phospho-KdpE binding site
type: SequenceAnnotation
persistent identity: annotation2473591
display id: annotation2473591
version: 1
top level: BBa_K1682003
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1682003/annotation2473591/range2473591/1

https://synbiohub.org/public/igem/BBa_K1682003/annotation2473591/range2473591/1
type: Range
persistent identity: range2473591
display id: range2473591
version: 1
direction: direction/forward
top level: BBa_K1682003
start: 8
end: 24
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2473590
display id: annotation2473590
version: 1
top level: BBa_K1682003
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1682003/annotation2473590/range2473590/1

https://synbiohub.org/public/igem/BBa_K1682003/annotation2473590/range2473590/1
type: Range
persistent identity: range2473590
display id: range2473590
version: 1
direction: direction/forward
top level: BBa_K1682003
start: 42
end: 47
orientation: inline

BBa_K862001
type: ComponentDefinition
persistent identity: BBa K862001
display id: BBa_K862001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: psulA-LacZ-doubleTerminator
creator: Charlotte Bunne, Mariam Harmouche, Stefan Holderbach, Anna Huhn and Jakob Kreft (in alphabetical order)
created: 2012-06-07T11:00:00Z
modified: 2015-05-08T01:13:36Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1122_
m user id: 0
owner id: 3317
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This is a part for the precise quantification of UV-radiation or radioactive radiation in E. coli (recA+) strains, i.e. BL21(DE3). It consits of a SulA Promoter (part BBa_K518010) fused to a LacZ reporter cloned in front of a double terminator (part BBa_K173004).
mutable notes: The part was first constructed in plasmid backbone pSB1AK3 and then subcloned into pSB1C3 for submission to the registry.
mutable provenance: The part was constructed by standard cloning from parts BBa_K518010 (pSulA) and part BBa_K173004 (LacZ reporter).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_K173004
component: BBa_K518010
sequence annotation: BBa_K173004
sequence annotation: BBa_K518010
sequence: https://synbiohub.org/public/igem/BBa_K862001_sequence/1

BBa_K173004
type: Component
persistent identity: component2176179
display id: component2176179
version: 1
top level: BBa_K862001
access: public
definition: https://synbiohub.org/public/igem/BBa_K173004/1

BBa_K518010
type: Component
persistent identity: component2176165
display id: component2176165
version: 1
top level: BBa_K862001
access: public
definition: https://synbiohub.org/public/igem/BBa_K518010/1

BBa_K173004
type: SequenceAnnotation
persistent identity: annotation2176179
display id: annotation2176179
version: 1
top level: BBa_K862001
location: https://synbiohub.org/public/igem/BBa_K862001/annotation2176179/range2176179/1
component: BBa_K173004

https://synbiohub.org/public/igem/BBa_K862001/annotation2176179/range2176179/1
type: Range
persistent identity: range2176179
display id: range2176179
version: 1
direction: direction/reverse
top level: BBa_K862001
start: 61
end: 3290
orientation: inline

BBa_K518010
type: SequenceAnnotation
persistent identity: annotation2176165
display id: annotation2176165
version: 1
top level: BBa_K862001
location: https://synbiohub.org/public/igem/BBa_K862001/annotation2176165/range2176165/1
component: BBa_K518010

https://synbiohub.org/public/igem/BBa_K862001/annotation2176165/range2176165/1
type: Range
persistent identity: range2176165
display id: range2176165
version: 1
direction: direction/reverse
top level: BBa_K862001
start: 1
end: 52
orientation: inline

BY-Toggle
type: ComponentDefinition
persistent identity: BBa K1908000
display id: BBa_K1908000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Blue-Yellow fluorescent protein toggle switch using pLac and OmpC promoter.
creator: Ryan Kawalerski, Sarah Heacox, Jonathan Krog
created: 2016-10-13T11:00:00Z
modified: 2016-10-18T11:04:57Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2374_
m user id: 29538
owner id: 29544
owning group id: 9
status: status/Planning
bookmark: true
mutable description: .
mutable notes: .
mutable provenance: .
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0015
component: BBa_J61100
component: BBa_K1033910
component: BBa_C0012
component: BBa_R0082
component: BBa_K592009
component: BBa_R0011
sequence annotation: BBa_K1033910
sequence annotation: BBa_B0015
sequence annotation: BBa_C0012
sequence annotation: BBa_K592009
sequence annotation: BBa_R0082
sequence annotation: BBa_R0011
sequence annotation: BBa_J61100
sequence: https://synbiohub.org/public/igem/BBa_K1908000_sequence/1

BBa_B0015
type: Component
persistent identity: component2520427
display id: component2520427
version: 1
top level: BY-Toggle
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_J61100
type: Component
persistent identity: component2520418
display id: component2520418
version: 1
top level: BY-Toggle
access: public
definition: https://synbiohub.org/public/igem/BBa_J61100/1

BBa_K1033910
type: Component
persistent identity: component2520420
display id: component2520420
version: 1
top level: BY-Toggle
access: public
definition: https://synbiohub.org/public/igem/BBa_K1033910/1

BBa_C0012
type: Component
persistent identity: component2520438
display id: component2520438
version: 1
top level: BY-Toggle
access: public
definition: https://synbiohub.org/public/igem/BBa_C0012/1

BBa_R0082
type: Component
persistent identity: component2520433
display id: component2520433
version: 1
top level: BY-Toggle
access: public
definition: https://synbiohub.org/public/igem/BBa_R0082/1

BBa_J61100
type: Component
persistent identity: component2520434
display id: component2520434
version: 1
top level: BY-Toggle
access: public
definition: https://synbiohub.org/public/igem/BBa_J61100/1

BBa_K592009
type: Component
persistent identity: component2520436
display id: component2520436
version: 1
top level: BY-Toggle
access: public
definition: https://synbiohub.org/public/igem/BBa_K592009/1

BBa_B0015
type: Component
persistent identity: component2520447
display id: component2520447
version: 1
top level: BY-Toggle
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_R0011
type: Component
persistent identity: component2520413
display id: component2520413
version: 1
top level: BY-Toggle
access: public
definition: https://synbiohub.org/public/igem/BBa_R0011/1

BBa_K1033910
type: SequenceAnnotation
persistent identity: annotation2520420
display id: annotation2520420
version: 1
top level: BY-Toggle
location: https://synbiohub.org/public/igem/BBa_K1908000/annotation2520420/range2520420/1
component: BBa_K1033910

https://synbiohub.org/public/igem/BBa_K1908000/annotation2520420/range2520420/1
type: Range
persistent identity: range2520420
display id: range2520420
version: 1
direction: direction/reverse
top level: BY-Toggle
start: 82
end: 795
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2520447
display id: annotation2520447
version: 1
top level: BY-Toggle
location: https://synbiohub.org/public/igem/BBa_K1908000/annotation2520447/range2520447/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K1908000/annotation2520447/range2520447/1
type: Range
persistent identity: range2520447
display id: range2520447
version: 1
direction: direction/reverse
top level: BY-Toggle
start: 2911
end: 3039
orientation: inline

BBa_C0012
type: SequenceAnnotation
persistent identity: annotation2520438
display id: annotation2520438
version: 1
top level: BY-Toggle
location: https://synbiohub.org/public/igem/BBa_K1908000/annotation2520438/range2520438/1
component: BBa_C0012

https://synbiohub.org/public/igem/BBa_K1908000/annotation2520438/range2520438/1
type: Range
persistent identity: range2520438
display id: range2520438
version: 1
direction: direction/forward
top level: BY-Toggle
start: 1750
end: 2877
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2520427
display id: annotation2520427
version: 1
top level: BY-Toggle
location: https://synbiohub.org/public/igem/BBa_K1908000/annotation2520427/range2520427/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K1908000/annotation2520427/range2520427/1
type: Range
persistent identity: range2520427
display id: range2520427
version: 1
direction: direction/reverse
top level: BY-Toggle
start: 804
end: 932
orientation: inline

BBa_K592009
type: SequenceAnnotation
persistent identity: annotation2520436
display id: annotation2520436
version: 1
top level: BY-Toggle
location: https://synbiohub.org/public/igem/BBa_K1908000/annotation2520436/range2520436/1
component: BBa_K592009

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type: Range
persistent identity: range2520436
display id: range2520436
version: 1
direction: direction/reverse
top level: BY-Toggle
start: 1075
end: 1743
orientation: inline

BBa_R0082
type: SequenceAnnotation
persistent identity: annotation2520433
display id: annotation2520433
version: 1
top level: BY-Toggle
location: https://synbiohub.org/public/igem/BBa_K1908000/annotation2520433/range2520433/1
component: BBa_R0082

https://synbiohub.org/public/igem/BBa_K1908000/annotation2520433/range2520433/1
type: Range
persistent identity: range2520433
display id: range2520433
version: 1
direction: direction/reverse
top level: BY-Toggle
start: 941
end: 1048
orientation: inline

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation2520413
display id: annotation2520413
version: 1
top level: BY-Toggle
location: https://synbiohub.org/public/igem/BBa_K1908000/annotation2520413/range2520413/1
component: BBa_R0011

https://synbiohub.org/public/igem/BBa_K1908000/annotation2520413/range2520413/1
type: Range
persistent identity: range2520413
display id: range2520413
version: 1
direction: direction/forward
top level: BY-Toggle
start: 1
end: 54
orientation: inline

BBa_J61100
type: SequenceAnnotation
persistent identity: annotation2520418
display id: annotation2520418
version: 1
top level: BY-Toggle
location: https://synbiohub.org/public/igem/BBa_K1908000/annotation2520418/range2520418/1
component: BBa_J61100

https://synbiohub.org/public/igem/BBa_K1908000/annotation2520418/range2520418/1
type: Range
persistent identity: range2520418
display id: range2520418
version: 1
direction: direction/reverse
top level: BY-Toggle
start: 64
end: 75
orientation: inline

BBa_J61100
type: SequenceAnnotation
persistent identity: annotation2520434
display id: annotation2520434
version: 1
top level: BY-Toggle
location: https://synbiohub.org/public/igem/BBa_K1908000/annotation2520434/range2520434/1
component: BBa_J61100

https://synbiohub.org/public/igem/BBa_K1908000/annotation2520434/range2520434/1
type: Range
persistent identity: range2520434
display id: range2520434
version: 1
direction: direction/reverse
top level: BY-Toggle
start: 1057
end: 1068
orientation: inline

BBa_K1020012
type: ComponentDefinition
persistent identity: BBa K1020012
display id: BBa_K1020012
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Alkane Selector for intracellular alkane production
creator: Yue Liu
created: 2013-09-09T11:00:00Z
modified: 2015-05-08T01:08:43Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1327_
m user id: 0
owner id: 16881
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1020010
component: BBa_K1020003
sequence annotation: BBa_K1020003
sequence annotation: BBa_K1020010
sequence: https://synbiohub.org/public/igem/BBa_K1020012_sequence/1

BBa_K1020010
type: Component
persistent identity: component2373789
display id: component2373789
version: 1
top level: BBa_K1020012
access: public
definition: https://synbiohub.org/public/igem/BBa_K1020010/1

BBa_K1020003
type: Component
persistent identity: component2373802
display id: component2373802
version: 1
top level: BBa_K1020012
access: public
definition: BBa_K1020003

BBa_K1020003
type: SequenceAnnotation
persistent identity: annotation2373802
display id: annotation2373802
version: 1
top level: BBa_K1020012
location: https://synbiohub.org/public/igem/BBa_K1020012/annotation2373802/range2373802/1
component: BBa_K1020003

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type: Range
persistent identity: range2373802
display id: range2373802
version: 1
direction: direction/reverse
top level: BBa_K1020012
start: 1538
end: 2662
orientation: inline

BBa_K1020010
type: SequenceAnnotation
persistent identity: annotation2373789
display id: annotation2373789
version: 1
top level: BBa_K1020012
location: https://synbiohub.org/public/igem/BBa_K1020012/annotation2373789/range2373789/1
component: BBa_K1020010

https://synbiohub.org/public/igem/BBa_K1020012/annotation2373789/range2373789/1
type: Range
persistent identity: range2373789
display id: range2373789
version: 1
direction: direction/reverse
top level: BBa_K1020012
start: 1
end: 1529
orientation: inline

BBa_K1497022
type: ComponentDefinition
persistent identity: BBa K1497022
display id: BBa_K1497022
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Naringenin sensor (FdeR) with CFP as reporter
creator: Sascha Hein, Niklas Hummel, Sebastian Barthel, Thomas Dohmen
created: 2014-10-06T11:00:00Z
modified: 2015-05-08T01:10:45Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1877_
m user id: 0
owner id: 12466
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: We modified the naringenin biosensor from Siedler et al. by elimination of one EcoRI restriction site and by changing the native ribosomal binding site into the strong RBS Bba_B0034. Based on this reporter protein and 3 different fluorescence proteins, we designed 3 new biosensors for in vivo detection and determination of naringenin. The natural source of FdeR is Herbaspirillum seropedicae. You can use our reporter for measuring naringenin concentrations in your samples. Depending on which fluorophor you want to detect, you can use one of three biosensors: GFP fluorescence K1497020 mKate (DsRed variant) fluorescence K1497021 CFP fluorescence K1497022
mutable notes: d
mutable provenance: d
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_K1497019
component: BBa_E0020
sequence annotation: BBa_E0020
sequence annotation: BBa_K1497019
sequence: https://synbiohub.org/public/igem/BBa_K1497022_sequence/1

BBa_K1497019
type: Component
persistent identity: component2398956
display id: component2398956
version: 1
top level: BBa_K1497022
access: public
definition: BBa_K1497019

BBa_E0020
type: Component
persistent identity: component2398957
display id: component2398957
version: 1
top level: BBa_K1497022
access: public
definition: https://synbiohub.org/public/igem/BBa_E0020/1

BBa_E0020
type: SequenceAnnotation
persistent identity: annotation2398957
display id: annotation2398957
version: 1
top level: BBa_K1497022
location: https://synbiohub.org/public/igem/BBa_K1497022/annotation2398957/range2398957/1
component: BBa_E0020

https://synbiohub.org/public/igem/BBa_K1497022/annotation2398957/range2398957/1
type: Range
persistent identity: range2398957
display id: range2398957
version: 1
direction: direction/reverse
top level: BBa_K1497022
start: 1221
end: 1943
orientation: inline

BBa_K1497019
type: SequenceAnnotation
persistent identity: annotation2398956
display id: annotation2398956
version: 1
top level: BBa_K1497022
location: https://synbiohub.org/public/igem/BBa_K1497022/annotation2398956/range2398956/1
component: BBa_K1497019

https://synbiohub.org/public/igem/BBa_K1497022/annotation2398956/range2398956/1
type: Range
persistent identity: range2398956
display id: range2398956
version: 1
direction: direction/reverse
top level: BBa_K1497022
start: 1
end: 1214
orientation: inline

BBa_K862000
type: ComponentDefinition
persistent identity: BBa K862000
display id: BBa_K862000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: precA-LacZ-doubleTerminator
creator: Charlotte Bunne, Mariam Harmouche, Stefan Holderbach, Anna Huhn and Jakob Kreft (in alphabetical order)
created: 2012-06-07T11:00:00Z
modified: 2015-05-08T01:13:36Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1122_
m user id: 0
owner id: 3317
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This is a part for the precise quantification of UV-radiation or radioactive radiation in E. coli (rec+) strains, i.e. BL21(DE3). It consits of a recA Promoter (part BBa_J22106) fused to a LacZ reporter cloned in front of a double terminator (part BBa_K173004).
mutable notes: The part was first constructed in plasmid backbone pSB1AK3 and then subcloned into pSB1C3 for submission to the registry.
mutable provenance: The part was constructed by standard cloning from parts BBa_J22106 (pRecA) and part BBa_K173004 (LacZ reporter).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_J22106
component: BBa_K173004
sequence annotation: BBa_K173004
sequence annotation: BBa_J22106
sequence: https://synbiohub.org/public/igem/BBa_K862000_sequence/1

BBa_J22106
type: Component
persistent identity: component2176150
display id: component2176150
version: 1
top level: BBa_K862000
access: public
definition: https://synbiohub.org/public/igem/BBa_J22106/1

BBa_K173004
type: Component
persistent identity: component2176164
display id: component2176164
version: 1
top level: BBa_K862000
access: public
definition: https://synbiohub.org/public/igem/BBa_K173004/1

BBa_K173004
type: SequenceAnnotation
persistent identity: annotation2176164
display id: annotation2176164
version: 1
top level: BBa_K862000
location: https://synbiohub.org/public/igem/BBa_K862000/annotation2176164/range2176164/1
component: BBa_K173004

https://synbiohub.org/public/igem/BBa_K862000/annotation2176164/range2176164/1
type: Range
persistent identity: range2176164
display id: range2176164
version: 1
direction: direction/reverse
top level: BBa_K862000
start: 201
end: 3430
orientation: inline

BBa_J22106
type: SequenceAnnotation
persistent identity: annotation2176150
display id: annotation2176150
version: 1
top level: BBa_K862000
location: https://synbiohub.org/public/igem/BBa_K862000/annotation2176150/range2176150/1
component: BBa_J22106

https://synbiohub.org/public/igem/BBa_K862000/annotation2176150/range2176150/1
type: Range
persistent identity: range2176150
display id: range2176150
version: 1
direction: direction/reverse
top level: BBa_K862000
start: 1
end: 192
orientation: inline

Ateam
type: ComponentDefinition
persistent identity: BBa K1023003
display id: BBa_K1023003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: FRET based ATP biosensor cds, codon optimized for Aspergillus niger
creator: Shreyans Chordia (shreyanschordia90@gmail.com)
created: 2013-09-16T11:00:00Z
modified: 2015-05-08T01:08:45Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1330_
m user id: 0
owner id: 15730
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: A FRET based biosensor that will measure the ratio of ATP and ADP.
mutable provenance: Aspergillus niger
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1023003_sequence/1

BBa_K1315002
type: ComponentDefinition
persistent identity: BBa K1315002
display id: BBa_K1315002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: The rpfC gene from Xanthomonas campestris
creator: Dimitrios Michailidis
created: 2014-09-24T11:00:00Z
modified: 2015-05-08T01:09:48Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1690_
m user id: 0
owner id: 21812
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This is the coding sequence for the RpfC(regulation of pathogenicity factor C) protein. It is the sensor in the two-component signal transduction of Xcc that activates the regulator RpfG(Bba_K1315003) in the presence of DSF.
mutable notes: The sequence had 1 EcoRI site and 3 PstI sites that were all mutated out by site-directed mutagenesis.
mutable provenance: The source of this part is ''Xanthomonas campestris pv. campestris''(Xcc) genomic DNA which was provided by the Robert Ryan lab in the Molecular Microbiology department of the University of Dundee, UK.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: RpfC
sequence: https://synbiohub.org/public/igem/BBa_K1315002_sequence/1

RpfC
type: SequenceAnnotation
persistent identity: annotation2386328
display id: annotation2386328
version: 1
top level: BBa_K1315002
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1315002/annotation2386328/range2386328/1

https://synbiohub.org/public/igem/BBa_K1315002/annotation2386328/range2386328/1
type: Range
persistent identity: range2386328
display id: range2386328
version: 1
direction: direction/forward
top level: BBa_K1315002
start: 1
end: 2181
orientation: inline

BBa_K1020004
type: ComponentDefinition
persistent identity: BBa K1020004
display id: BBa_K1020004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Biosensor for alkane detection
creator: Yue Liu
created: 2013-08-17T11:00:00Z
modified: 2015-05-08T01:08:43Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1327_
m user id: 0
owner id: 16881
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: AlkR is a transcriptional regulator belonging to AraC/Xyls family, which could detect a broad range of alkanes and alkenes with carbon chain length from C7 to C36. It is said that AlkR is the only bioreporter that is able to detect alkane with carbon chain length greater than C18. The mechanism is that Alkane were presumably recognized by AlkR regulatory protein and their interaction triggered a conformation change of AlkR dimers which isomerizes the promoter-RNAP complex and lead to the activation of alkM gene. AlkR regulatory protein includes C-terminal DNA-binding domain(CTD) for promoter binding and N-terminal domain(NTD) for inducer recognition.
mutable notes: We choise a constitutive promoter for AlkR gene and in case of interference, we use a long and strong terminator.
mutable provenance: Acinetobacter baylyi ADP1
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_J23100
component: BBa_K1020002
component: BBa_K1020001
component: BBa_B0034
sequence annotation: BBa_K1020002
sequence annotation: BBa_B0034
sequence annotation: BBa_J23100
sequence annotation: BBa_K1020001
sequence: https://synbiohub.org/public/igem/BBa_K1020004_sequence/1

BBa_J23100
type: Component
persistent identity: component2331904
display id: component2331904
version: 1
top level: BBa_K1020004
access: public
definition: https://synbiohub.org/public/igem/BBa_J23100/1

BBa_K1020002
type: Component
persistent identity: component2331908
display id: component2331908
version: 1
top level: BBa_K1020004
access: public
definition: BBa_K1020002

BBa_K1020001
type: Component
persistent identity: component2331903
display id: component2331903
version: 1
top level: BBa_K1020004
access: public
definition: https://synbiohub.org/public/igem/BBa_K1020001/1

BBa_B0034
type: Component
persistent identity: component2331906
display id: component2331906
version: 1
top level: BBa_K1020004
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K1020002
type: SequenceAnnotation
persistent identity: annotation2331908
display id: annotation2331908
version: 1
top level: BBa_K1020004
location: https://synbiohub.org/public/igem/BBa_K1020004/annotation2331908/range2331908/1
component: BBa_K1020002

https://synbiohub.org/public/igem/BBa_K1020004/annotation2331908/range2331908/1
type: Range
persistent identity: range2331908
display id: range2331908
version: 1
direction: direction/reverse
top level: BBa_K1020004
start: 1083
end: 2009
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2331906
display id: annotation2331906
version: 1
top level: BBa_K1020004
location: https://synbiohub.org/public/igem/BBa_K1020004/annotation2331906/range2331906/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1020004/annotation2331906/range2331906/1
type: Range
persistent identity: range2331906
display id: range2331906
version: 1
direction: direction/reverse
top level: BBa_K1020004
start: 1065
end: 1076
orientation: inline

BBa_J23100
type: SequenceAnnotation
persistent identity: annotation2331904
display id: annotation2331904
version: 1
top level: BBa_K1020004
location: https://synbiohub.org/public/igem/BBa_K1020004/annotation2331904/range2331904/1
component: BBa_J23100

https://synbiohub.org/public/igem/BBa_K1020004/annotation2331904/range2331904/1
type: Range
persistent identity: range2331904
display id: range2331904
version: 1
direction: direction/reverse
top level: BBa_K1020004
start: 1022
end: 1056
orientation: inline

BBa_K1020001
type: SequenceAnnotation
persistent identity: annotation2331903
display id: annotation2331903
version: 1
top level: BBa_K1020004
location: https://synbiohub.org/public/igem/BBa_K1020004/annotation2331903/range2331903/1
component: BBa_K1020001

https://synbiohub.org/public/igem/BBa_K1020004/annotation2331903/range2331903/1
type: Range
persistent identity: range2331903
display id: range2331903
version: 1
direction: direction/reverse
top level: BBa_K1020004
start: 1
end: 1013
orientation: inline

BBa_K1682009
type: ComponentDefinition
persistent identity: BBa K1682009
display id: BBa_K1682009
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PkdpF[-15, T>G] - GFP generator
creator: iGEM15_HKUST-Rice
created: 2015-08-10T11:00:00Z
modified: 2015-09-18T09:11:00Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2100_
m user id: 8758
owner id: 21235
owning group id: 9
status: status/Planning
bookmark: false
mutable description: kdpFp[-15, T>G] - E0240
mutable notes: kdpFp[-15, T>G] - E0240
mutable provenance: kdpFp[-15, T>G] - E0240
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_K1682004
component: BBa_E0240
sequence annotation: BBa_K1682004
sequence annotation: BBa_E0240
sequence: https://synbiohub.org/public/igem/BBa_K1682009_sequence/1

BBa_K1682004
type: Component
persistent identity: component2434955
display id: component2434955
version: 1
top level: BBa_K1682009
access: public
definition: BBa_K1682004

BBa_E0240
type: Component
persistent identity: component2434966
display id: component2434966
version: 1
top level: BBa_K1682009
access: public
definition: https://synbiohub.org/public/igem/BBa_E0240/1

BBa_K1682004
type: SequenceAnnotation
persistent identity: annotation2434955
display id: annotation2434955
version: 1
top level: BBa_K1682009
location: https://synbiohub.org/public/igem/BBa_K1682009/annotation2434955/range2434955/1
component: BBa_K1682004

https://synbiohub.org/public/igem/BBa_K1682009/annotation2434955/range2434955/1
type: Range
persistent identity: range2434955
display id: range2434955
version: 1
direction: direction/reverse
top level: BBa_K1682009
start: 1
end: 78
orientation: inline

BBa_E0240
type: SequenceAnnotation
persistent identity: annotation2434966
display id: annotation2434966
version: 1
top level: BBa_K1682009
location: https://synbiohub.org/public/igem/BBa_K1682009/annotation2434966/range2434966/1
component: BBa_E0240

https://synbiohub.org/public/igem/BBa_K1682009/annotation2434966/range2434966/1
type: Range
persistent identity: range2434966
display id: range2434966
version: 1
direction: direction/reverse
top level: BBa_K1682009
start: 87
end: 962
orientation: inline

precB
type: ComponentDefinition
persistent identity: BBa K862003
display id: BBa_K862003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: precB
creator: Charlotte Bunne, Mariam Harmouche, Stefan Holderbach, Anna Huhn and Jakob Kreft (in alphabetical order)
created: 2012-06-07T11:00:00Z
modified: 2015-05-08T01:13:36Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1122_
m user id: 0
owner id: 3317
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: The recB promoter sequence was taken for the E. coli MG1655 genome sequence (http://ecoliwiki.net/colipedia/index.php/recB:Gene). We assumed the main promoter region to be located from -70 to -1 bp upstream the recB start codon. Therefore this sequence was synthesized and cloned on an oligo basis. We will not submit the physical DNA of this part, but we provide the oligo-sequences you can use for synthesizing and cloning this part in front of any EcoRI/XbaI precut reporter part. RecB_fw: aattcgcggccgcttctagagCCTGAAGGCTGGAAAGTGTGGGAGAACGTCAGCGCGTTGCAGCAAACAATGCCCCTGATGAGTGAAAAGAc RecB_rev: ctaggTCTTTTCACTCATCAGGGGCATTGTTTGCTGCAACGCGCTGACGTTCTCCCACACTTTCCAGCCTTCAGGctctagaagcggccgcg
mutable notes: the sequence was ordered on a synthetic oligo basis and cloned in front of EcoRI/XbaI reporter parts.
mutable provenance: E. coli MG1655 genome: http://ecoliwiki.net/colipedia/index.php/recB:Gene bp -70 to -1 before recB coding sequence.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K862003_sequence/1

BBa_K1682002
type: ComponentDefinition
persistent identity: BBa K1682002
display id: BBa_K1682002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PkdpF[-15, T>A]
creator: iGEM15_HKUST-Rice
created: 2015-08-09T11:00:00Z
modified: 2015-09-18T09:15:41Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2100_
m user id: 8758
owner id: 21235
owning group id: 9
status: status/Planning
bookmark: false
mutable description: kdpFp[-15, T>A]
mutable notes: kdpFp[-15, T>A]
mutable provenance: kdpFp[-15, T>A]
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -10
sequence annotation: phospho-KdpE binding site
sequence annotation: EcoRI Illegal site removed T->A
sequence annotation: -35
sequence: https://synbiohub.org/public/igem/BBa_K1682002_sequence/1

-10
type: SequenceAnnotation
persistent identity: annotation2473586
display id: annotation2473586
version: 1
top level: BBa_K1682002
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1682002/annotation2473586/range2473586/1

https://synbiohub.org/public/igem/BBa_K1682002/annotation2473586/range2473586/1
type: Range
persistent identity: range2473586
display id: range2473586
version: 1
direction: direction/forward
top level: BBa_K1682002
start: 69
end: 74
orientation: inline

phospho-KdpE binding site
type: SequenceAnnotation
persistent identity: annotation2473585
display id: annotation2473585
version: 1
top level: BBa_K1682002
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1682002/annotation2473585/range2473585/1

https://synbiohub.org/public/igem/BBa_K1682002/annotation2473585/range2473585/1
type: Range
persistent identity: range2473585
display id: range2473585
version: 1
direction: direction/forward
top level: BBa_K1682002
start: 8
end: 24
orientation: inline

EcoRI Illegal site removed T->A
type: SequenceAnnotation
persistent identity: annotation2475205
display id: annotation2475205
version: 1
top level: BBa_K1682002
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K1682002/annotation2475205/range2475205/1

https://synbiohub.org/public/igem/BBa_K1682002/annotation2475205/range2475205/1
type: Range
persistent identity: range2475205
display id: range2475205
version: 1
direction: direction/forward
top level: BBa_K1682002
start: 65
end: 66
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2473587
display id: annotation2473587
version: 1
top level: BBa_K1682002
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1682002/annotation2473587/range2473587/1

https://synbiohub.org/public/igem/BBa_K1682002/annotation2473587/range2473587/1
type: Range
persistent identity: range2473587
display id: range2473587
version: 1
direction: direction/forward
top level: BBa_K1682002
start: 42
end: 47
orientation: inline

BBa_K1682012
type: ComponentDefinition
persistent identity: BBa K1682012
display id: BBa_K1682012
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PphoA promoter
creator: iGEM15_HKUST-Rice
created: 2015-09-01T11:00:00Z
modified: 2015-09-18T10:16:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2100_
m user id: 21235
owner id: 26069
owning group id: 9
status: status/Planning
bookmark: false
mutable description: PphoA promoter is a phosphate-responsive promoter. In high concentration of phosphate, it will be repressed while in low phosphate concentration, it will be activated.
mutable notes: N/A
mutable provenance: Native in E. coli
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -10
sequence annotation: -35
sequence annotation: phospho-phoB
sequence: https://synbiohub.org/public/igem/BBa_K1682012_sequence/1

-10
type: SequenceAnnotation
persistent identity: annotation2476004
display id: annotation2476004
version: 1
top level: BBa_K1682012
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1682012/annotation2476004/range2476004/1

https://synbiohub.org/public/igem/BBa_K1682012/annotation2476004/range2476004/1
type: Range
persistent identity: range2476004
display id: range2476004
version: 1
direction: direction/forward
top level: BBa_K1682012
start: 50
end: 56
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2476003
display id: annotation2476003
version: 1
top level: BBa_K1682012
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1682012/annotation2476003/range2476003/1

https://synbiohub.org/public/igem/BBa_K1682012/annotation2476003/range2476003/1
type: Range
persistent identity: range2476003
display id: range2476003
version: 1
direction: direction/forward
top level: BBa_K1682012
start: 28
end: 33
orientation: inline

phospho-phoB
type: SequenceAnnotation
persistent identity: annotation2476002
display id: annotation2476002
version: 1
top level: BBa_K1682012
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1682012/annotation2476002/range2476002/1

https://synbiohub.org/public/igem/BBa_K1682012/annotation2476002/range2476002/1
type: Range
persistent identity: range2476002
display id: range2476002
version: 1
direction: direction/forward
top level: BBa_K1682012
start: 21
end: 40
orientation: inline

BBa_K1170000
type: ComponentDefinition
persistent identity: BBa K1170000
display id: BBa_K1170000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: asr (acid shock response) promoter
creator: Rohit Satija
created: 2013-09-01T11:00:00Z
modified: 2015-05-08T01:09:34Z
discontinued: false
dominant: false
group u list: _1483_
m user id: 0
owner id: 16731
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: An acid inducible promoter of the acid shock response (asr) gene, extracted from E. coli K12. This part can be used to generate pH based response in any biological system.
mutable notes: The sequence provided in literature indicated a consensus sequence for the Pho Box just before the transcription start site
mutable provenance: This part was extracted from the genome of E. coli K12
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: asr promoter
sequence annotation: TATA box
sequence annotation: Pho Box
sequence: https://synbiohub.org/public/igem/BBa_K1170000_sequence/1

asr promoter
type: SequenceAnnotation
persistent identity: annotation2334962
display id: annotation2334962
version: 1
top level: BBa_K1170000
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1170000/annotation2334962/range2334962/1

https://synbiohub.org/public/igem/BBa_K1170000/annotation2334962/range2334962/1
type: Range
persistent identity: range2334962
display id: range2334962
version: 1
direction: direction/forward
top level: BBa_K1170000
start: 1
end: 178
orientation: inline

TATA box
type: SequenceAnnotation
persistent identity: annotation2334963
display id: annotation2334963
version: 1
top level: BBa_K1170000
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K1170000/annotation2334963/range2334963/1

https://synbiohub.org/public/igem/BBa_K1170000/annotation2334963/range2334963/1
type: Range
persistent identity: range2334963
display id: range2334963
version: 1
direction: direction/forward
top level: BBa_K1170000
start: 141
end: 147
orientation: inline

Pho Box
type: SequenceAnnotation
persistent identity: annotation2334964
display id: annotation2334964
version: 1
top level: BBa_K1170000
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K1170000/annotation2334964/range2334964/1

https://synbiohub.org/public/igem/BBa_K1170000/annotation2334964/range2334964/1
type: Range
persistent identity: range2334964
display id: range2334964
version: 1
direction: direction/forward
top level: BBa_K1170000
start: 114
end: 131
orientation: inline

IscA1
type: ComponentDefinition
persistent identity: BBa M36907
display id: BBa_M36907
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Coding Sequence of IscA1 pigeon homologue (codon-optimized for humans)
creator: Michael Becich, Thomas Lau, Sreyas Misra
created: 2015-10-24T11:00:00Z
modified: 2015-12-04T07:45:15Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _848_
m user id: 29123
owner id: 29123
owning group id: 9
status: status/Planning
bookmark: true
mutable description: The gene Iron-Sulfur cluster assembly 1 (IscA1) is a mitochondrial protein involved in the biogenesis and assembly of iron-sulfur clusters, which play a role in electron-transfer reactions and is theorized to be associated with certain organisms' magnetic perception. The homing pigeon is one such organism with this ability, and here we have codon-optimized the Columba Livia homologue for homo sapiens. The sequence can be inserted into plasmids for expression of the magnetoreceptor IscA gene, which hopefully will create magnetogenetic biosensors in the form of iron-sulfur cluster, for downstream output and applications.
mutable notes: While the plasmid design for this insert can be complicated, the coding sequence itself is quite simple. We only needed to remove the start codon in order to co-express a fluorescent protein, and modify two BbsII restriction enzyme sites by altering the third base pair of codons (wobble effect) at bp locations 75 (C->G) and 309 (A->G).
mutable provenance: The predicted IscA amino acid sequence was obtained from NCBI (http://www.ncbi.nlm.nih.gov/protein/XP_005508102.1), and codon-optimized for humans with IDT's tools.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_M36907_sequence/1

BBa_K1020002
type: ComponentDefinition
persistent identity: BBa K1020002
display id: BBa_K1020002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: alkane regulatory protein ALKR
creator: Yue Liu
created: 2013-08-17T11:00:00Z
modified: 2015-05-08T01:08:43Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1327_
m user id: 0
owner id: 16881
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: AlkR is a transcriptional regulator belonging to AraC/Xyls family, which could detect a broad range of alkanes and alkenes with carbon chain length from C7 to C36. It is said that AlkR is the only bioreporter that is able to detect alkane with carbon chain length greater than C18.
mutable provenance: Acinetobacter baylyi ADP1
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: AlkR
sequence: https://synbiohub.org/public/igem/BBa_K1020002_sequence/1

AlkR
type: SequenceAnnotation
persistent identity: annotation2331899
display id: annotation2331899
version: 1
top level: BBa_K1020002
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1020002/annotation2331899/range2331899/1

https://synbiohub.org/public/igem/BBa_K1020002/annotation2331899/range2331899/1
type: Range
persistent identity: range2331899
display id: range2331899
version: 1
direction: direction/forward
top level: BBa_K1020002
start: 1
end: 927
orientation: inline

BBa_K1682011
type: ComponentDefinition
persistent identity: BBa K1682011
display id: BBa_K1682011
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: NsrR
creator: iGEM15_HKUST-Rice
created: 2015-09-01T11:00:00Z
modified: 2015-09-18T09:29:08Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2100_
m user id: 8758
owner id: 26069
owning group id: 9
status: status/Planning
bookmark: false
mutable description: NsrR protein is a nitrate sensitive transcriptional repressor. When there is nitrate, it will be oxidized to nitric oxide by nitrate oxidase, which in turns relieve the repression of NsrR on PyeaR promoter.
mutable notes: N/A
mutable provenance: Native in E. coli
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: nsrR
sequence: https://synbiohub.org/public/igem/BBa_K1682011_sequence/1

nsrR
type: SequenceAnnotation
persistent identity: annotation2475311
display id: annotation2475311
version: 1
top level: BBa_K1682011
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1682011/annotation2475311/range2475311/1

https://synbiohub.org/public/igem/BBa_K1682011/annotation2475311/range2475311/1
type: Range
persistent identity: range2475311
display id: range2475311
version: 1
direction: direction/forward
top level: BBa_K1682011
start: 1
end: 426
orientation: inline

BBa_K1497021
type: ComponentDefinition
persistent identity: BBa K1497021
display id: BBa_K1497021
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Naringenin sensor (FdeR) with mKate as reporter
creator: Sascha Hein, Niklas Hummel, Sebastian Barthel, Thomas Dohmen
created: 2014-10-06T11:00:00Z
modified: 2015-05-08T01:10:45Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1877_
m user id: 0
owner id: 12466
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: We modified the naringenin biosensor from Siedler et al. by elimination of one EcoRI restriction site and by changing the native ribosomal binding site into the strong RBS Bba_B0034. Based on this reporter protein and 3 different fluorescence proteins, we designed 3 new biosensors for in vivo detection and determination of naringenin. The natural source of FdeR is Herbaspirillum seropedicae. You can use our reporter for measuring naringenin concentrations in your samples. Depending on which fluorophor you want to detect, you can use one of three biosensors: GFP fluorescence K1497020 mKate (DsRed variant) fluorescence K1497021 CFP fluorescence K1497022
mutable notes: d
mutable provenance: d
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_K1497019
component: BBa_K1055000
sequence annotation: BBa_K1497019
sequence annotation: BBa_K1055000
sequence: https://synbiohub.org/public/igem/BBa_K1497021_sequence/1

BBa_K1497019
type: Component
persistent identity: component2398954
display id: component2398954
version: 1
top level: BBa_K1497021
access: public
definition: BBa_K1497019

BBa_K1055000
type: Component
persistent identity: component2398955
display id: component2398955
version: 1
top level: BBa_K1497021
access: public
definition: https://synbiohub.org/public/igem/BBa_K1055000/1

BBa_K1497019
type: SequenceAnnotation
persistent identity: annotation2398954
display id: annotation2398954
version: 1
top level: BBa_K1497021
location: https://synbiohub.org/public/igem/BBa_K1497021/annotation2398954/range2398954/1
component: BBa_K1497019

https://synbiohub.org/public/igem/BBa_K1497021/annotation2398954/range2398954/1
type: Range
persistent identity: range2398954
display id: range2398954
version: 1
direction: direction/reverse
top level: BBa_K1497021
start: 1
end: 1214
orientation: inline

BBa_K1055000
type: SequenceAnnotation
persistent identity: annotation2398955
display id: annotation2398955
version: 1
top level: BBa_K1497021
location: https://synbiohub.org/public/igem/BBa_K1497021/annotation2398955/range2398955/1
component: BBa_K1055000

https://synbiohub.org/public/igem/BBa_K1497021/annotation2398955/range2398955/1
type: Range
persistent identity: range2398955
display id: range2398955
version: 1
direction: direction/reverse
top level: BBa_K1497021
start: 1221
end: 1937
orientation: inline