//function/reporter/light
type: Collection
persistent identity: function reporter light
display id: function_reporter_light
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
owned by: james
owned by: myers
member: BBa_K389015
member: BBa_K389411
member: BBa_J52012
member: BBa_K1486018
member: BBa_K819000
member: SgsE | Luc
member: BBa_J52008
member: BBa_K1486027
member: BBa_K1689009
member: BBa_K1486055
member: SP-AntiNIP
member: BBa_K1486020
member: BBa_K1761005
member: BBa_K518013
member: BBa_K424003
member: Tm-Splitlu
member: BBa_K389012
member: BBa_K284999
member: BBa_K389413
member: BBa_K389318
member: BBa_K819008
member: BBa_K1680010
member: BBa_K424004
member: BBa_K1486016
member: BBa_K216014
member: Luc
member: BBa_K1486036
member: BBa_K389401
member: BBa_K2023003
member: BBa_K1734004
member: BBa_K1486017
member: BBa_K389307
member: BBa_J52011
member: BBa_K1486022
member: BBa_K1616025
member: BBa_K518002
member: BBa_K389402
member: BBa_K1680025
member: BBa_K1689004
member: BBa_K216008
member: BBa_K1680009
member: BBa_K389403
member: BBa_K2023009
member: BBa_K1486021
member: BBa_K1732027
member: BBa_K389412
member: BBa_K950004
member: Rluc
member: BBa_K389421
member: BBa_K325905
member: BBa_K785003
member: BBa_I712019
member: BBa_J52013
member: BBa_K1486019
member: BBa_K1486033
member: BBa_K1761002
member: BBa_K389051
member: BBa_K1689006
member: BBa_K1761006
member: BBa_K1486054
member: fLuc
member: BBa_K2023006
member: BBa_K322237
member: luc
member: Tm-Split-L
member: BBa_K1689001
member: BBa_K216015
member: SP-Lipocal
member: BBa_K389422
member: BBa_K1159001
member: BBa_K1732003
member: BB123

BBa_K1486022
type: ComponentDefinition
persistent identity: BBa K1486022
display id: BBa_K1486022
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Renilla Luciferase
creator: EPFL iGem team 2014
created: 2014-09-14T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This part is the full renilla luciferase. The split protein has also been submitted as biobricks: BBa_K1486021.
mutable notes: to edit
mutable provenance: To edit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K1486020
component: BBa_K1486019
component: BBa_I0500
component: BBa_B0034
sequence annotation: BBa_I0500
sequence annotation: BBa_B0034
sequence annotation: BBa_K1486019
sequence annotation: BBa_K1486020
sequence: https://synbiohub.org/public/igem/BBa_K1486022_sequence/1

BBa_K1486020
type: Component
persistent identity: component2383475
display id: component2383475
version: 1
top level: BBa_K1486022
access: public
definition: BBa_K1486020

BBa_K1486019
type: Component
persistent identity: component2383474
display id: component2383474
version: 1
top level: BBa_K1486022
access: public
definition: BBa_K1486019

BBa_I0500
type: Component
persistent identity: component2383471
display id: component2383471
version: 1
top level: BBa_K1486022
access: public
definition: https://synbiohub.org/public/igem/BBa_I0500/1

BBa_B0034
type: Component
persistent identity: component2383473
display id: component2383473
version: 1
top level: BBa_K1486022
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_I0500
type: SequenceAnnotation
persistent identity: annotation2383471
display id: annotation2383471
version: 1
top level: BBa_K1486022
location: https://synbiohub.org/public/igem/BBa_K1486022/annotation2383471/range2383471/1
component: BBa_I0500

https://synbiohub.org/public/igem/BBa_K1486022/annotation2383471/range2383471/1
type: Range
persistent identity: range2383471
display id: range2383471
version: 1
direction: direction/reverse
top level: BBa_K1486022
start: 1
end: 1210
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2383473
display id: annotation2383473
version: 1
top level: BBa_K1486022
location: https://synbiohub.org/public/igem/BBa_K1486022/annotation2383473/range2383473/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1486022/annotation2383473/range2383473/1
type: Range
persistent identity: range2383473
display id: range2383473
version: 1
direction: direction/reverse
top level: BBa_K1486022
start: 1211
end: 1222
orientation: inline

BBa_K1486019
type: SequenceAnnotation
persistent identity: annotation2383474
display id: annotation2383474
version: 1
top level: BBa_K1486022
location: https://synbiohub.org/public/igem/BBa_K1486022/annotation2383474/range2383474/1
component: BBa_K1486019

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type: Range
persistent identity: range2383474
display id: range2383474
version: 1
direction: direction/reverse
top level: BBa_K1486022
start: 1223
end: 1552
orientation: inline

BBa_K1486020
type: SequenceAnnotation
persistent identity: annotation2383475
display id: annotation2383475
version: 1
top level: BBa_K1486022
location: https://synbiohub.org/public/igem/BBa_K1486022/annotation2383475/range2383475/1
component: BBa_K1486020

https://synbiohub.org/public/igem/BBa_K1486022/annotation2383475/range2383475/1
type: Range
persistent identity: range2383475
display id: range2383475
version: 1
direction: direction/reverse
top level: BBa_K1486022
start: 1553
end: 2158
orientation: inline

BBa_K389015
type: ComponentDefinition
persistent identity: BBa K389015
display id: BBa_K389015
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: VirA/G reporter device luc
creator: Jonas Aretz
created: 2010-09-27T11:00:00Z
modified: 2015-05-08T01:12:19Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _517_
m user id: 0
owner id: 6670
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This BioBrick is for testing the virA/G signaling system. The read-out is the luciferase <partinfo>K389004</partinfo>. This system contains an unmutated ''virA'', so it is possible to measure, how the natural virA/G system works and reacts.
mutable notes: this is only for measuring the natural virA/G system
mutable provenance: ''A. tumefaciens'' C58 TI-plasmid, partsregistry, Mr. Gene, Promega's pGL4.10luc2 plasmid
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K389012
component: BBa_K389010
sequence annotation: BBa_K389012
sequence annotation: BBa_K389010
sequence: https://synbiohub.org/public/igem/BBa_K389015_sequence/1

BBa_K389012
type: Component
persistent identity: component2227573
display id: component2227573
version: 1
top level: BBa_K389015
access: public
definition: BBa_K389012

BBa_K389010
type: Component
persistent identity: component2227555
display id: component2227555
version: 1
top level: BBa_K389015
access: public
definition: https://synbiohub.org/public/igem/BBa_K389010/1

BBa_K389012
type: SequenceAnnotation
persistent identity: annotation2227573
display id: annotation2227573
version: 1
top level: BBa_K389015
location: https://synbiohub.org/public/igem/BBa_K389015/annotation2227573/range2227573/1
component: BBa_K389012

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type: Range
persistent identity: range2227573
display id: range2227573
version: 1
direction: direction/reverse
top level: BBa_K389015
start: 2575
end: 5361
orientation: inline

BBa_K389010
type: SequenceAnnotation
persistent identity: annotation2227555
display id: annotation2227555
version: 1
top level: BBa_K389015
location: https://synbiohub.org/public/igem/BBa_K389015/annotation2227555/range2227555/1
component: BBa_K389010

https://synbiohub.org/public/igem/BBa_K389015/annotation2227555/range2227555/1
type: Range
persistent identity: range2227555
display id: range2227555
version: 1
direction: direction/reverse
top level: BBa_K389015
start: 1
end: 2566
orientation: inline

BBa_J52012
type: ComponentDefinition
persistent identity: BBa J52012
display id: BBa_J52012
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Rluc-linker-PEST191
creator: Monika Ciglic
created: 2006-10-13T11:00:00Z
modified: 2015-08-31T03:54:04Z
discontinued: false
dominant: false
group u list: _80_
m user id: 0
owner id: 800
owning group id: 80
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Rluc-linker-PEST191 is a part composed of Rluc (which acts as a reporter) linked with PEST191 through six aminoacid long protein linker. PEST191 is a sequence that leads towards degradation of protein linked with this sequence.
mutable notes: Rluc-linker-PEST 191 was made by using PCR ligation technique. This part is cloned in BioBrick vector pSB1AK3.
mutable provenance: -
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: PEST191
sequence annotation: linker
sequence annotation: Rluc
sequence annotation: Rluc-linker-PEST191
sequence: https://synbiohub.org/public/igem/BBa_J52012_sequence/1

PEST191
type: SequenceAnnotation
persistent identity: annotation1902839
display id: annotation1902839
version: 1
top level: BBa_J52012
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_J52012/annotation1902839/range1902839/1

https://synbiohub.org/public/igem/BBa_J52012/annotation1902839/range1902839/1
type: Range
persistent identity: range1902839
display id: range1902839
version: 1
direction: direction/forward
top level: BBa_J52012
start: 952
end: 1437
orientation: inline

linker
type: SequenceAnnotation
persistent identity: annotation1902838
display id: annotation1902838
version: 1
top level: BBa_J52012
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_J52012/annotation1902838/range1902838/1

https://synbiohub.org/public/igem/BBa_J52012/annotation1902838/range1902838/1
type: Range
persistent identity: range1902838
display id: range1902838
version: 1
direction: direction/forward
top level: BBa_J52012
start: 934
end: 951
orientation: inline

Rluc
type: SequenceAnnotation
persistent identity: annotation1902837
display id: annotation1902837
version: 1
top level: BBa_J52012
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J52012/annotation1902837/range1902837/1

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type: Range
persistent identity: range1902837
display id: range1902837
version: 1
direction: direction/forward
top level: BBa_J52012
start: 1
end: 933
orientation: inline

Rluc-linker-PEST191
type: SequenceAnnotation
persistent identity: annotation1902847
display id: annotation1902847
version: 1
top level: BBa_J52012
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_J52012/annotation1902847/range1902847/1

https://synbiohub.org/public/igem/BBa_J52012/annotation1902847/range1902847/1
type: Range
persistent identity: range1902847
display id: range1902847
version: 1
direction: direction/forward
top level: BBa_J52012
start: 1
end: 1437
orientation: inline

Luc
type: ComponentDefinition
persistent identity: BBa K525999
display id: BBa_K525999
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Firefly -Luciferase
creator: Anna Drong
created: 2011-09-12T11:00:00Z
modified: 2015-05-08T01:12:35Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _690_
m user id: 0
owner id: 8543
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: Luciferase from pGL3 ; RCU 25
mutable notes: Assembled
mutable provenance: BBa_I712019
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: NgoMIV
sequence annotation: Firefly luciferase
sequence annotation: TAA
sequence annotation: AgeI
sequence annotation: ATG
sequence: https://synbiohub.org/public/igem/BBa_K525999_sequence/1

NgoMIV
type: SequenceAnnotation
persistent identity: annotation2130013
display id: annotation2130013
version: 1
top level: Luc
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K525999/annotation2130013/range2130013/1

https://synbiohub.org/public/igem/BBa_K525999/annotation2130013/range2130013/1
type: Range
persistent identity: range2130013
display id: range2130013
version: 1
direction: direction/forward
top level: Luc
start: 4
end: 9
orientation: inline

Firefly luciferase
type: SequenceAnnotation
persistent identity: annotation2130011
display id: annotation2130011
version: 1
top level: Luc
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K525999/annotation2130011/range2130011/1

https://synbiohub.org/public/igem/BBa_K525999/annotation2130011/range2130011/1
type: Range
persistent identity: range2130011
display id: range2130011
version: 1
direction: direction/forward
top level: Luc
start: 1
end: 1665
orientation: inline

TAA
type: SequenceAnnotation
persistent identity: annotation2130015
display id: annotation2130015
version: 1
top level: Luc
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K525999/annotation2130015/range2130015/1

https://synbiohub.org/public/igem/BBa_K525999/annotation2130015/range2130015/1
type: Range
persistent identity: range2130015
display id: range2130015
version: 1
direction: direction/forward
top level: Luc
start: 1663
end: 1665
orientation: inline

AgeI
type: SequenceAnnotation
persistent identity: annotation2130014
display id: annotation2130014
version: 1
top level: Luc
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K525999/annotation2130014/range2130014/1

https://synbiohub.org/public/igem/BBa_K525999/annotation2130014/range2130014/1
type: Range
persistent identity: range2130014
display id: range2130014
version: 1
direction: direction/forward
top level: Luc
start: 1657
end: 1662
orientation: inline

ATG
type: SequenceAnnotation
persistent identity: annotation2130012
display id: annotation2130012
version: 1
top level: Luc
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K525999/annotation2130012/range2130012/1

https://synbiohub.org/public/igem/BBa_K525999/annotation2130012/range2130012/1
type: Range
persistent identity: range2130012
display id: range2130012
version: 1
direction: direction/forward
top level: Luc
start: 1
end: 3
orientation: inline

BBa_K1680010
type: ComponentDefinition
persistent identity: BBa K1680010
display id: BBa_K1680010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Firefly Luciferase
creator: Katharina Sporbeck
created: 2015-09-16T11:00:00Z
modified: 2015-10-27T01:37:31Z
discontinued: false
dominant: false
group u list: _2098_
m user id: 15693
owner id: 26460
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Protein coding region for the firefly [Photinus pyralis] luciferase, codon optimized for yeast.
mutable notes: Shipped in RFC 10
mutable provenance: gBlock designed by Team Tuebingen
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Luciferase
sequence: https://synbiohub.org/public/igem/BBa_K1680010_sequence/1

Luciferase
type: SequenceAnnotation
persistent identity: annotation2477414
display id: annotation2477414
version: 1
top level: BBa_K1680010
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1680010/annotation2477414/range2477414/1

https://synbiohub.org/public/igem/BBa_K1680010/annotation2477414/range2477414/1
type: Range
persistent identity: range2477414
display id: range2477414
version: 1
direction: direction/forward
top level: BBa_K1680010
start: 1
end: 1653
orientation: inline

BBa_K389302
type: ComponentDefinition
persistent identity: BBa K389302
display id: BBa_K389302
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: weak constitutive promoter + luc
creator: Jonas Aretz
created: 2010-10-11T11:00:00Z
modified: 2015-05-08T01:12:19Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _517_
m user id: 0
owner id: 6670
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This is a firefly luciferase gene under the control of a weak constitutive promoter.
mutable notes: weak promoter, strong RBS, used this to demonstrate that our luciferase BioBrick is working
mutable provenance: * promoter and RBS from partsregistry * luciferase from pGL4.10luc2 (Promega)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_J23103
component: BBa_B0034
component: BBa_K389004
sequence annotation: BBa_J23103
sequence annotation: BBa_K389004
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K389302_sequence/1

BBa_J23103
type: Component
persistent identity: component2085573
display id: component2085573
version: 1
top level: BBa_K389302
access: public
definition: https://synbiohub.org/public/igem/BBa_J23103/1

BBa_B0034
type: Component
persistent identity: component2085575
display id: component2085575
version: 1
top level: BBa_K389302
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K389004
type: Component
persistent identity: component2085578
display id: component2085578
version: 1
top level: BBa_K389302
access: public
definition: luc

BBa_J23103
type: SequenceAnnotation
persistent identity: annotation2085573
display id: annotation2085573
version: 1
top level: BBa_K389302
location: https://synbiohub.org/public/igem/BBa_K389302/annotation2085573/range2085573/1
component: BBa_J23103

https://synbiohub.org/public/igem/BBa_K389302/annotation2085573/range2085573/1
type: Range
persistent identity: range2085573
display id: range2085573
version: 1
direction: direction/reverse
top level: BBa_K389302
start: 1
end: 35
orientation: inline

BBa_K389004
type: SequenceAnnotation
persistent identity: annotation2085578
display id: annotation2085578
version: 1
top level: BBa_K389302
location: https://synbiohub.org/public/igem/BBa_K389302/annotation2085578/range2085578/1
component: BBa_K389004

https://synbiohub.org/public/igem/BBa_K389302/annotation2085578/range2085578/1
type: Range
persistent identity: range2085578
display id: range2085578
version: 1
direction: direction/reverse
top level: BBa_K389302
start: 62
end: 1717
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2085575
display id: annotation2085575
version: 1
top level: BBa_K389302
location: https://synbiohub.org/public/igem/BBa_K389302/annotation2085575/range2085575/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K389302/annotation2085575/range2085575/1
type: Range
persistent identity: range2085575
display id: range2085575
version: 1
direction: direction/reverse
top level: BBa_K389302
start: 44
end: 55
orientation: inline

BBa_K1486019
type: ComponentDefinition
persistent identity: BBa K1486019
display id: BBa_K1486019
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: rLuc[1]
creator: EPFL iGem team 2014
created: 2014-09-14T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: his is the first part of the split Renilla luciferase. To be used with fLuc[2].
mutable notes: to edit
mutable provenance: to edit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence: https://synbiohub.org/public/igem/BBa_K1486019_sequence/1

BBa_K216008
type: ComponentDefinition
persistent identity: BBa K216008
display id: BBa_K216008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: bacterial luciferase LuxAB of Xenorhabdus luminescens
creator: Edinburgh iGEM 2009
created: 2009-10-15T11:00:00Z
modified: 2015-05-08T01:11:30Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _317_
m user id: 0
owner id: 837
owning group id: 163
sample status: In stock
status: status/Available
bookmark: true
mutable description: This part includes the coding sequences of ''luxA'' and ''luxB'' of ''Xenorhabdus luminescens'', with their native ribosome binding sites. The dimeric bacterial luciferase LuxAB produces light in the presence of oxygen, FMNH2, and a long chain aldehyde. The aldehyde can be provided by the ''luxCDE'' gene products, but if these are not present, n-decanal can be added to cultures.
mutable notes: No special considerations. Please note that preliminary sequencing suggests that there may be a problem with the SpeI site of the construct submitted. If this is confirmed, we will submit a revised version and remove this note.
mutable provenance: Amplified from genomic DNA of ''Xenorhabdus luminescens''.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
sequence annotation: rbs
sequence annotation: start luxB
sequence annotation: luxA coding sequence
sequence annotation: luxB coding sequence
sequence annotation: end luxB
sequence annotation: end luxA
sequence annotation: start luxA
sequence: https://synbiohub.org/public/igem/BBa_K216008_sequence/1

rbs
type: SequenceAnnotation
persistent identity: annotation2046870
display id: annotation2046870
version: 1
top level: BBa_K216008
role: feature/rbs
role: SO:0000139
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display id: range2046870
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start: 15
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start luxB
type: SequenceAnnotation
persistent identity: annotation2046875
display id: annotation2046875
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top level: BBa_K216008
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K216008/annotation2046875/range2046875/1

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type: Range
persistent identity: range2046875
display id: range2046875
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start: 1124
end: 1126
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luxA coding sequence
type: SequenceAnnotation
persistent identity: annotation2046873
display id: annotation2046873
version: 1
top level: BBa_K216008
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K216008/annotation2046873/range2046873/1

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persistent identity: range2046873
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start: 27
end: 1109
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luxB coding sequence
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persistent identity: annotation2046877
display id: annotation2046877
version: 1
top level: BBa_K216008
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K216008/annotation2046877/range2046877/1

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persistent identity: range2046877
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version: 1
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start: 1124
end: 2107
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end luxB
type: SequenceAnnotation
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display id: annotation2046876
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role: SO:0000319
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start: 2105
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end luxA
type: SequenceAnnotation
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display id: annotation2046872
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role: SO:0000319
role: feature/stop
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persistent identity: range2046872
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direction: direction/forward
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start: 1107
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orientation: inline

start luxA
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role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K216008/annotation2046871/range2046871/1

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type: Range
persistent identity: range2046871
display id: range2046871
version: 1
direction: direction/forward
top level: BBa_K216008
start: 27
end: 29
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation2046874
display id: annotation2046874
version: 1
top level: BBa_K216008
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K216008/annotation2046874/range2046874/1

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persistent identity: range2046874
display id: range2046874
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direction: direction/forward
top level: BBa_K216008
start: 1112
end: 1117
orientation: inline

Rluc
type: ComponentDefinition
persistent identity: BBa K1722005
display id: BBa_K1722005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Rluc can express Renilla luciferase（Rluc）.
creator: Kexin Li
created: 2015-08-28T11:00:00Z
modified: 2016-01-19T01:27:56Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2142_
m user id: 4206
owner id: 27226
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Rluc can express Renilla luciferase which has become popular as a reporter enzyme for gene expression assays. Renilla luciferase (RLUC)is a blue-light emitting luciferase of marine anthozoan Renilla reniformis [Str].As a reporter gene, rescarchers attach it to a regulatory sequence of another gene of interest in bacteria, cell culture, animals or plants. RLUC is chosen as a reporter because the characteristic it confer on organisms expressing it is easily identified and measured.The bioluminescence of the sea pancy,is under the control of a nerve network and is stimlated by changes of intracellular Ca??﹢ concerntramide，CO₂ andlight(λMax=480nm),as in the following scheme. RLBP is another protein of Renilla reniformis that can luminesce in vivo called Ca??﹢???triggered Renilla luciferin birding protein. 2015 SZU-IGEM construct Rluc with one codon being amber mutated and SV40 ,the promoter in the same plasmid to introduce Rluc into our system.This plasmid with Rluc,together with two other plasmids,are inserted into the cell.Only when the two promoters in plasmids without Rluc are activated simultanuously to express the downstrean DNA sequence can Rluc being translated completely. RLUC that is produced inside the cell catalyzes a reaction with luciferin to produce light. By measuring the light intensity using Luminometer,we can tell the working efficiency of our system under different conditions.
mutable notes: We designed the following primers and amplified Rluc promoter from the vector psi-Check2:Up: CCGGAATTCTCTAGACTGGCTGCCAAGTAGTAC Down: TGCACTGCAGACTAGTTACTGCTCGTTCTT. By incorporating these primers into Rluc promoter, the promoter is flanked by the iGEM prefix and suffix after amplification.
mutable provenance: In our experiment, we got the part from Shenzhen Second People's Hospital. Additionally, we did our system's function verification in Shenzhen Second People's Hospital.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_K1722005_sequence/1

BBa_K424004
type: ComponentDefinition
persistent identity: BBa K424004
display id: BBa_K424004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LuxC gene from V. fischeri for generating light.
creator: Patrick Nee
created: 2010-06-06T11:00:00Z
modified: 2015-05-08T01:12:28Z
discontinued: true
dominant: false
experience: experience/None
group u list: _545_
m user id: 0
owner id: 6272
owning group id: 9
sample status: Discontinued
status: status/Deleted
bookmark: false
mutable description: Which species is the enzyme from? Vibrio fischeri What reaction does it catalyze? One of five steps in the production of light. Does it work in E. coli? Yes, several groups have used this operon as a reporter in E. coli. Does it require any other parts? Yes. The full luxCDABE operon is needed for light production.
mutable notes: Requires the other four steps in the LuxABCDE process
mutable provenance: ACCESSION Y00509 REGION: 1624..1866 AUTHORS Engebrecht,J. and Silverman,M. TITLE Nucleotide sequence of the regulatory locus controlling expression of bacterial genes for bioluminescence JOURNAL Nucleic Acids Res. 15 (24), 10455-10467 (1987) PUBMED 3697093
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Start
sequence annotation: LuxC protien
sequence annotation: TaaTaa
sequence: https://synbiohub.org/public/igem/BBa_K424004_sequence/1

Start
type: SequenceAnnotation
persistent identity: annotation2070322
display id: annotation2070322
version: 1
top level: BBa_K424004
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K424004/annotation2070322/range2070322/1

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persistent identity: range2070322
display id: range2070322
version: 1
direction: direction/forward
top level: BBa_K424004
start: 23
end: 29
orientation: inline

LuxC protien
type: SequenceAnnotation
persistent identity: annotation2070323
display id: annotation2070323
version: 1
top level: BBa_K424004
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K424004/annotation2070323/range2070323/1

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type: Range
persistent identity: range2070323
display id: range2070323
version: 1
direction: direction/forward
top level: BBa_K424004
start: 35
end: 224
orientation: inline

TaaTaa
type: SequenceAnnotation
persistent identity: annotation2070321
display id: annotation2070321
version: 1
top level: BBa_K424004
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K424004/annotation2070321/range2070321/1

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persistent identity: range2070321
display id: range2070321
version: 1
direction: direction/forward
top level: BBa_K424004
start: 246
end: 252
orientation: inline

BBa_K1486036
type: ComponentDefinition
persistent identity: BBa K1486036
display id: BBa_K1486036
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: rLucC + ADH1 terminator + CaUra3 cassette
creator: EPFL iGem team 2014
created: 2014-09-26T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: To edit
mutable notes: To edit
mutable provenance: To edit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000324
role: partType/Tag
component: BBa_K1486031
component: BBa_K1486037
component: BBa_K1486025
component: BBa_K1486020
sequence annotation: BBa_K1486037
sequence annotation: BBa_K1486025
sequence annotation: BBa_K1486020
sequence annotation: BBa_K1486031
sequence: https://synbiohub.org/public/igem/BBa_K1486036_sequence/1

BBa_K1486031
type: Component
persistent identity: component2418343
display id: component2418343
version: 1
top level: BBa_K1486036
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486031/1

BBa_K1486037
type: Component
persistent identity: component2418337
display id: component2418337
version: 1
top level: BBa_K1486036
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486037/1

BBa_K1486025
type: Component
persistent identity: component2418339
display id: component2418339
version: 1
top level: BBa_K1486036
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486025/1

BBa_K1486020
type: Component
persistent identity: component2418338
display id: component2418338
version: 1
top level: BBa_K1486036
access: public
definition: BBa_K1486020

BBa_K1486037
type: SequenceAnnotation
persistent identity: annotation2418337
display id: annotation2418337
version: 1
top level: BBa_K1486036
location: https://synbiohub.org/public/igem/BBa_K1486036/annotation2418337/range2418337/1
component: BBa_K1486037

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persistent identity: range2418337
display id: range2418337
version: 1
direction: direction/reverse
top level: BBa_K1486036
start: 1
end: 39
orientation: inline

BBa_K1486025
type: SequenceAnnotation
persistent identity: annotation2418339
display id: annotation2418339
version: 1
top level: BBa_K1486036
location: https://synbiohub.org/public/igem/BBa_K1486036/annotation2418339/range2418339/1
component: BBa_K1486025

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persistent identity: range2418339
display id: range2418339
version: 1
direction: direction/reverse
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start: 646
end: 833
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BBa_K1486020
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persistent identity: annotation2418338
display id: annotation2418338
version: 1
top level: BBa_K1486036
location: https://synbiohub.org/public/igem/BBa_K1486036/annotation2418338/range2418338/1
component: BBa_K1486020

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type: Range
persistent identity: range2418338
display id: range2418338
version: 1
direction: direction/reverse
top level: BBa_K1486036
start: 40
end: 645
orientation: inline

BBa_K1486031
type: SequenceAnnotation
persistent identity: annotation2418343
display id: annotation2418343
version: 1
top level: BBa_K1486036
location: https://synbiohub.org/public/igem/BBa_K1486036/annotation2418343/range2418343/1
component: BBa_K1486031

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type: Range
persistent identity: range2418343
display id: range2418343
version: 1
direction: direction/reverse
top level: BBa_K1486036
start: 834
end: 2193
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BBa_K1486054
type: ComponentDefinition
persistent identity: BBa K1486054
display id: BBa_K1486054
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CheY/CheZ + split rLuc
creator: EPFL iGem team 2014
created: 2014-10-06T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: to edit
mutable notes: to edit
mutable provenance: to edit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_K569017
component: BBa_K1486020
component: BBa_K1486019
component: BBa_B0034
component: BBa_K1486053
component: BBa_K629003
component: BBa_I0500
sequence annotation: BBa_K629003
sequence annotation: BBa_K569017
sequence annotation: BBa_K1486020
sequence annotation: BBa_B0034
sequence annotation: BBa_K1486019
sequence annotation: BBa_K1486053
sequence annotation: BBa_I0500
sequence: https://synbiohub.org/public/igem/BBa_K1486054_sequence/1

BBa_K569017
type: Component
persistent identity: component2399862
display id: component2399862
version: 1
top level: BBa_K1486054
access: public
definition: https://synbiohub.org/public/igem/BBa_K569017/1

BBa_K1486020
type: Component
persistent identity: component2399875
display id: component2399875
version: 1
top level: BBa_K1486054
access: public
definition: BBa_K1486020

BBa_K1486019
type: Component
persistent identity: component2399864
display id: component2399864
version: 1
top level: BBa_K1486054
access: public
definition: BBa_K1486019

BBa_B0034
type: Component
persistent identity: component2399866
display id: component2399866
version: 1
top level: BBa_K1486054
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0034
type: Component
persistent identity: component2399861
display id: component2399861
version: 1
top level: BBa_K1486054
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K1486053
type: Component
persistent identity: component2399863
display id: component2399863
version: 1
top level: BBa_K1486054
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486053/1

BBa_K629003
type: Component
persistent identity: component2399873
display id: component2399873
version: 1
top level: BBa_K1486054
access: public
definition: https://synbiohub.org/public/igem/BBa_K629003/1

BBa_K1486053
type: Component
persistent identity: component2399874
display id: component2399874
version: 1
top level: BBa_K1486054
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486053/1

BBa_I0500
type: Component
persistent identity: component2399859
display id: component2399859
version: 1
top level: BBa_K1486054
access: public
definition: https://synbiohub.org/public/igem/BBa_I0500/1

BBa_K629003
type: SequenceAnnotation
persistent identity: annotation2399873
display id: annotation2399873
version: 1
top level: BBa_K1486054
location: https://synbiohub.org/public/igem/BBa_K1486054/annotation2399873/range2399873/1
component: BBa_K629003

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type: Range
persistent identity: range2399873
display id: range2399873
version: 1
direction: direction/reverse
top level: BBa_K1486054
start: 2013
end: 2657
orientation: inline

BBa_K569017
type: SequenceAnnotation
persistent identity: annotation2399862
display id: annotation2399862
version: 1
top level: BBa_K1486054
location: https://synbiohub.org/public/igem/BBa_K1486054/annotation2399862/range2399862/1
component: BBa_K569017

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type: Range
persistent identity: range2399862
display id: range2399862
version: 1
direction: direction/reverse
top level: BBa_K1486054
start: 1223
end: 1640
orientation: inline

BBa_K1486020
type: SequenceAnnotation
persistent identity: annotation2399875
display id: annotation2399875
version: 1
top level: BBa_K1486054
location: https://synbiohub.org/public/igem/BBa_K1486054/annotation2399875/range2399875/1
component: BBa_K1486020

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type: Range
persistent identity: range2399875
display id: range2399875
version: 1
direction: direction/reverse
top level: BBa_K1486054
start: 2688
end: 3293
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2399866
display id: annotation2399866
version: 1
top level: BBa_K1486054
location: https://synbiohub.org/public/igem/BBa_K1486054/annotation2399866/range2399866/1
component: BBa_B0034

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type: Range
persistent identity: range2399866
display id: range2399866
version: 1
direction: direction/reverse
top level: BBa_K1486054
start: 2001
end: 2012
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2399861
display id: annotation2399861
version: 1
top level: BBa_K1486054
location: https://synbiohub.org/public/igem/BBa_K1486054/annotation2399861/range2399861/1
component: BBa_B0034

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type: Range
persistent identity: range2399861
display id: range2399861
version: 1
direction: direction/reverse
top level: BBa_K1486054
start: 1211
end: 1222
orientation: inline

BBa_K1486019
type: SequenceAnnotation
persistent identity: annotation2399864
display id: annotation2399864
version: 1
top level: BBa_K1486054
location: https://synbiohub.org/public/igem/BBa_K1486054/annotation2399864/range2399864/1
component: BBa_K1486019

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type: Range
persistent identity: range2399864
display id: range2399864
version: 1
direction: direction/reverse
top level: BBa_K1486054
start: 1671
end: 2000
orientation: inline

BBa_K1486053
type: SequenceAnnotation
persistent identity: annotation2399874
display id: annotation2399874
version: 1
top level: BBa_K1486054
location: https://synbiohub.org/public/igem/BBa_K1486054/annotation2399874/range2399874/1
component: BBa_K1486053

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type: Range
persistent identity: range2399874
display id: range2399874
version: 1
direction: direction/reverse
top level: BBa_K1486054
start: 2658
end: 2687
orientation: inline

BBa_I0500
type: SequenceAnnotation
persistent identity: annotation2399859
display id: annotation2399859
version: 1
top level: BBa_K1486054
location: https://synbiohub.org/public/igem/BBa_K1486054/annotation2399859/range2399859/1
component: BBa_I0500

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type: Range
persistent identity: range2399859
display id: range2399859
version: 1
direction: direction/reverse
top level: BBa_K1486054
start: 1
end: 1210
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BBa_K1486053
type: SequenceAnnotation
persistent identity: annotation2399863
display id: annotation2399863
version: 1
top level: BBa_K1486054
location: https://synbiohub.org/public/igem/BBa_K1486054/annotation2399863/range2399863/1
component: BBa_K1486053

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type: Range
persistent identity: range2399863
display id: range2399863
version: 1
direction: direction/reverse
top level: BBa_K1486054
start: 1641
end: 1670
orientation: inline

Tm-Split-L
type: ComponentDefinition
persistent identity: BBa K157020
display id: BBa_K157020
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Tm-Split-Luciferase1/2
creator: iGEM Team Freiburg 2008
created: 2008-10-25T11:00:00Z
modified: 2015-05-08T01:10:54Z
discontinued: false
dominant: false
experience: experience/None
group u list: _232_
m user id: 0
owner id: 1673
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Part 1 of the Split- Luciferase fused to the trans-membrane region of the Erbb1/EGF-receptor.
mutable notes: none
mutable provenance: Derived by cloning of the parts BBa_K157002 and Split-Luciferase fragment 1. For further information see the description of the parts mentioned above.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: AgeI
sequence annotation: Scar (Thr, Gly)
sequence annotation: Split-Luciferase1
sequence annotation: Tm
sequence annotation: Tm-Split-Luciferase1/2
sequence annotation: NgoMI
sequence: https://synbiohub.org/public/igem/BBa_K157020_sequence/1

AgeI
type: SequenceAnnotation
persistent identity: annotation1990038
display id: annotation1990038
version: 1
top level: Tm-Split-L
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157020/annotation1990038/range1990038/1

https://synbiohub.org/public/igem/BBa_K157020/annotation1990038/range1990038/1
type: Range
persistent identity: range1990038
display id: range1990038
version: 1
direction: direction/forward
top level: Tm-Split-L
start: 313
end: 318
orientation: inline

Scar (Thr, Gly)
type: SequenceAnnotation
persistent identity: annotation1991535
display id: annotation1991535
version: 1
top level: Tm-Split-L
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157020/annotation1991535/range1991535/1

https://synbiohub.org/public/igem/BBa_K157020/annotation1991535/range1991535/1
type: Range
persistent identity: range1991535
display id: range1991535
version: 1
direction: direction/forward
top level: Tm-Split-L
start: 79
end: 84
orientation: inline

Split-Luciferase1
type: SequenceAnnotation
persistent identity: annotation1991536
display id: annotation1991536
version: 1
top level: Tm-Split-L
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157020/annotation1991536/range1991536/1

https://synbiohub.org/public/igem/BBa_K157020/annotation1991536/range1991536/1
type: Range
persistent identity: range1991536
display id: range1991536
version: 1
direction: direction/forward
top level: Tm-Split-L
start: 85
end: 312
orientation: inline

Tm
type: SequenceAnnotation
persistent identity: annotation1991534
display id: annotation1991534
version: 1
top level: Tm-Split-L
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157020/annotation1991534/range1991534/1

https://synbiohub.org/public/igem/BBa_K157020/annotation1991534/range1991534/1
type: Range
persistent identity: range1991534
display id: range1991534
version: 1
direction: direction/forward
top level: Tm-Split-L
start: 10
end: 78
orientation: inline

Tm-Split-Luciferase1/2
type: SequenceAnnotation
persistent identity: annotation1990063
display id: annotation1990063
version: 1
top level: Tm-Split-L
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K157020/annotation1990063/range1990063/1

https://synbiohub.org/public/igem/BBa_K157020/annotation1990063/range1990063/1
type: Range
persistent identity: range1990063
display id: range1990063
version: 1
direction: direction/forward
top level: Tm-Split-L
start: 1
end: 318
orientation: inline

NgoMI
type: SequenceAnnotation
persistent identity: annotation1990037
display id: annotation1990037
version: 1
top level: Tm-Split-L
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157020/annotation1990037/range1990037/1

https://synbiohub.org/public/igem/BBa_K157020/annotation1990037/range1990037/1
type: Range
persistent identity: range1990037
display id: range1990037
version: 1
direction: direction/forward
top level: Tm-Split-L
start: 4
end: 9
orientation: inline

SgsE | Luc
type: ComponentDefinition
persistent identity: BBa K525311
display id: BBa_K525311
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Fusion Protein of S-Layer SgsE and Firefly-Luciferase
creator: Anna Drong
created: 2011-09-12T11:00:00Z
modified: 2015-05-08T01:12:34Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _690_
m user id: 0
owner id: 8543
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: PT7 + RBS + SgsE || luc
mutable notes: Assembled
mutable provenance: BBa_K525303; BBa_K525999
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: AgeI
sequence annotation: NgoMIV
sequence annotation: Luciferase
sequence annotation: B0034
sequence annotation: pT7
sequence annotation: SgsE domain
sequence annotation: lac opterator
sequence annotation: ATG
sequence annotation: TAA
sequence annotation: K525301
sequence annotation: RBS
sequence annotation: K525999
sequence annotation: Freiburg scar
sequence: https://synbiohub.org/public/igem/BBa_K525311_sequence/1

AgeI
type: SequenceAnnotation
persistent identity: annotation2137369
display id: annotation2137369
version: 1
top level: SgsE | Luc
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K525311/annotation2137369/range2137369/1

https://synbiohub.org/public/igem/BBa_K525311/annotation2137369/range2137369/1
type: Range
persistent identity: range2137369
display id: range2137369
version: 1
direction: direction/forward
top level: SgsE | Luc
start: 4054
end: 4059
orientation: inline

NgoMIV
type: SequenceAnnotation
persistent identity: annotation2137365
display id: annotation2137365
version: 1
top level: SgsE | Luc
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K525311/annotation2137365/range2137365/1

https://synbiohub.org/public/igem/BBa_K525311/annotation2137365/range2137365/1
type: Range
persistent identity: range2137365
display id: range2137365
version: 1
direction: direction/forward
top level: SgsE | Luc
start: 76
end: 81
orientation: inline

Luciferase
type: SequenceAnnotation
persistent identity: annotation2137366
display id: annotation2137366
version: 1
top level: SgsE | Luc
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K525311/annotation2137366/range2137366/1

https://synbiohub.org/public/igem/BBa_K525311/annotation2137366/range2137366/1
type: Range
persistent identity: range2137366
display id: range2137366
version: 1
direction: direction/forward
top level: SgsE | Luc
start: 2407
end: 4062
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation2137352
display id: annotation2137352
version: 1
top level: SgsE | Luc
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K525311/annotation2137352/range2137352/1

https://synbiohub.org/public/igem/BBa_K525311/annotation2137352/range2137352/1
type: Range
persistent identity: range2137352
display id: range2137352
version: 1
direction: direction/forward
top level: SgsE | Luc
start: 53
end: 64
orientation: inline

pT7
type: SequenceAnnotation
persistent identity: annotation2137343
display id: annotation2137343
version: 1
top level: SgsE | Luc
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K525311/annotation2137343/range2137343/1

https://synbiohub.org/public/igem/BBa_K525311/annotation2137343/range2137343/1
type: Range
persistent identity: range2137343
display id: range2137343
version: 1
direction: direction/forward
top level: SgsE | Luc
start: 1
end: 20
orientation: inline

SgsE domain
type: SequenceAnnotation
persistent identity: annotation2137354
display id: annotation2137354
version: 1
top level: SgsE | Luc
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K525311/annotation2137354/range2137354/1

https://synbiohub.org/public/igem/BBa_K525311/annotation2137354/range2137354/1
type: Range
persistent identity: range2137354
display id: range2137354
version: 1
direction: direction/forward
top level: SgsE | Luc
start: 73
end: 2400
orientation: inline

lac opterator
type: SequenceAnnotation
persistent identity: annotation2137351
display id: annotation2137351
version: 1
top level: SgsE | Luc
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K525311/annotation2137351/range2137351/1

https://synbiohub.org/public/igem/BBa_K525311/annotation2137351/range2137351/1
type: Range
persistent identity: range2137351
display id: range2137351
version: 1
direction: direction/forward
top level: SgsE | Luc
start: 20
end: 44
orientation: inline

ATG
type: SequenceAnnotation
persistent identity: annotation2137364
display id: annotation2137364
version: 1
top level: SgsE | Luc
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K525311/annotation2137364/range2137364/1

https://synbiohub.org/public/igem/BBa_K525311/annotation2137364/range2137364/1
type: Range
persistent identity: range2137364
display id: range2137364
version: 1
direction: direction/forward
top level: SgsE | Luc
start: 73
end: 75
orientation: inline

TAA
type: SequenceAnnotation
persistent identity: annotation2137368
display id: annotation2137368
version: 1
top level: SgsE | Luc
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K525311/annotation2137368/range2137368/1

https://synbiohub.org/public/igem/BBa_K525311/annotation2137368/range2137368/1
type: Range
persistent identity: range2137368
display id: range2137368
version: 1
direction: direction/forward
top level: SgsE | Luc
start: 4060
end: 4062
orientation: inline

K525301
type: SequenceAnnotation
persistent identity: annotation2137362
display id: annotation2137362
version: 1
top level: SgsE | Luc
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K525311/annotation2137362/range2137362/1

https://synbiohub.org/public/igem/BBa_K525311/annotation2137362/range2137362/1
type: Range
persistent identity: range2137362
display id: range2137362
version: 1
direction: direction/forward
top level: SgsE | Luc
start: 73
end: 2400
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2137353
display id: annotation2137353
version: 1
top level: SgsE | Luc
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K525311/annotation2137353/range2137353/1

https://synbiohub.org/public/igem/BBa_K525311/annotation2137353/range2137353/1
type: Range
persistent identity: range2137353
display id: range2137353
version: 1
direction: direction/forward
top level: SgsE | Luc
start: 56
end: 59
orientation: inline

K525999
type: SequenceAnnotation
persistent identity: annotation2137367
display id: annotation2137367
version: 1
top level: SgsE | Luc
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K525311/annotation2137367/range2137367/1

https://synbiohub.org/public/igem/BBa_K525311/annotation2137367/range2137367/1
type: Range
persistent identity: range2137367
display id: range2137367
version: 1
direction: direction/forward
top level: SgsE | Luc
start: 2407
end: 4062
orientation: inline

Freiburg scar
type: SequenceAnnotation
persistent identity: annotation2137363
display id: annotation2137363
version: 1
top level: SgsE | Luc
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K525311/annotation2137363/range2137363/1

https://synbiohub.org/public/igem/BBa_K525311/annotation2137363/range2137363/1
type: Range
persistent identity: range2137363
display id: range2137363
version: 1
direction: direction/forward
top level: SgsE | Luc
start: 2401
end: 2406
orientation: inline

BBa_K325905
type: ComponentDefinition
persistent identity: BBa K325905
display id: BBa_K325905
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Bacterial Lux reporter CDEG pλ AB (E. coli optimised)
creator: Will Handley and Emily Knott
created: 2010-10-22T11:00:00Z
modified: 2015-05-08T01:12:03Z
discontinued: false
dominant: false
experience: experience/Fails
group u list: _446_
m user id: 0
owner id: 7466
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: a
mutable notes: a
mutable provenance: a
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: rbs
sequence annotation: LuxA
sequence annotation: pLambda
sequence annotation: LuxC
sequence annotation: LuxD
sequence annotation: terminator
sequence annotation: LuxE
sequence annotation: LuxG
sequence annotation: LuxB
sequence: https://synbiohub.org/public/igem/BBa_K325905_sequence/1

rbs
type: SequenceAnnotation
persistent identity: annotation2109336
display id: annotation2109336
version: 1
top level: BBa_K325905
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K325905/annotation2109336/range2109336/1

https://synbiohub.org/public/igem/BBa_K325905/annotation2109336/range2109336/1
type: Range
persistent identity: range2109336
display id: range2109336
version: 1
direction: direction/forward
top level: BBa_K325905
start: 5480
end: 5485
orientation: inline

LuxA
type: SequenceAnnotation
persistent identity: annotation2109334
display id: annotation2109334
version: 1
top level: BBa_K325905
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K325905/annotation2109334/range2109334/1

https://synbiohub.org/public/igem/BBa_K325905/annotation2109334/range2109334/1
type: Range
persistent identity: range2109334
display id: range2109334
version: 1
direction: direction/forward
top level: BBa_K325905
start: 4395
end: 5477
orientation: inline

pLambda
type: SequenceAnnotation
persistent identity: annotation2109291
display id: annotation2109291
version: 1
top level: BBa_K325905
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K325905/annotation2109291/range2109291/1

https://synbiohub.org/public/igem/BBa_K325905/annotation2109291/range2109291/1
type: Range
persistent identity: range2109291
display id: range2109291
version: 1
direction: direction/forward
top level: BBa_K325905
start: 4320
end: 4368
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation2109258
display id: annotation2109258
version: 1
top level: BBa_K325905
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K325905/annotation2109258/range2109258/1

https://synbiohub.org/public/igem/BBa_K325905/annotation2109258/range2109258/1
type: Range
persistent identity: range2109258
display id: range2109258
version: 1
direction: direction/forward
top level: BBa_K325905
start: 3553
end: 3569
orientation: inline

LuxC
type: SequenceAnnotation
persistent identity: annotation2109254
display id: annotation2109254
version: 1
top level: BBa_K325905
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K325905/annotation2109254/range2109254/1

https://synbiohub.org/public/igem/BBa_K325905/annotation2109254/range2109254/1
type: Range
persistent identity: range2109254
display id: range2109254
version: 1
direction: direction/forward
top level: BBa_K325905
start: 18
end: 1457
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation2109242
display id: annotation2109242
version: 1
top level: BBa_K325905
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K325905/annotation2109242/range2109242/1

https://synbiohub.org/public/igem/BBa_K325905/annotation2109242/range2109242/1
type: Range
persistent identity: range2109242
display id: range2109242
version: 1
direction: direction/forward
top level: BBa_K325905
start: 1
end: 17
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation2109302
display id: annotation2109302
version: 1
top level: BBa_K325905
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K325905/annotation2109302/range2109302/1

https://synbiohub.org/public/igem/BBa_K325905/annotation2109302/range2109302/1
type: Range
persistent identity: range2109302
display id: range2109302
version: 1
direction: direction/forward
top level: BBa_K325905
start: 4383
end: 4388
orientation: inline

LuxD
type: SequenceAnnotation
persistent identity: annotation2109256
display id: annotation2109256
version: 1
top level: BBa_K325905
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K325905/annotation2109256/range2109256/1

https://synbiohub.org/public/igem/BBa_K325905/annotation2109256/range2109256/1
type: Range
persistent identity: range2109256
display id: range2109256
version: 1
direction: direction/forward
top level: BBa_K325905
start: 1476
end: 2398
orientation: inline

terminator
type: SequenceAnnotation
persistent identity: annotation2109262
display id: annotation2109262
version: 1
top level: BBa_K325905
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K325905/annotation2109262/range2109262/1

https://synbiohub.org/public/igem/BBa_K325905/annotation2109262/range2109262/1
type: Range
persistent identity: range2109262
display id: range2109262
version: 1
direction: direction/forward
top level: BBa_K325905
start: 4281
end: 4319
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation2109259
display id: annotation2109259
version: 1
top level: BBa_K325905
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K325905/annotation2109259/range2109259/1

https://synbiohub.org/public/igem/BBa_K325905/annotation2109259/range2109259/1
type: Range
persistent identity: range2109259
display id: range2109259
version: 1
direction: direction/forward
top level: BBa_K325905
start: 2399
end: 2415
orientation: inline

LuxE
type: SequenceAnnotation
persistent identity: annotation2109257
display id: annotation2109257
version: 1
top level: BBa_K325905
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K325905/annotation2109257/range2109257/1

https://synbiohub.org/public/igem/BBa_K325905/annotation2109257/range2109257/1
type: Range
persistent identity: range2109257
display id: range2109257
version: 1
direction: direction/forward
top level: BBa_K325905
start: 2416
end: 3552
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation2109255
display id: annotation2109255
version: 1
top level: BBa_K325905
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K325905/annotation2109255/range2109255/1

https://synbiohub.org/public/igem/BBa_K325905/annotation2109255/range2109255/1
type: Range
persistent identity: range2109255
display id: range2109255
version: 1
direction: direction/forward
top level: BBa_K325905
start: 1458
end: 1475
orientation: inline

LuxG
type: SequenceAnnotation
persistent identity: annotation2109260
display id: annotation2109260
version: 1
top level: BBa_K325905
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K325905/annotation2109260/range2109260/1

https://synbiohub.org/public/igem/BBa_K325905/annotation2109260/range2109260/1
type: Range
persistent identity: range2109260
display id: range2109260
version: 1
direction: direction/forward
top level: BBa_K325905
start: 3570
end: 4280
orientation: inline

LuxB
type: SequenceAnnotation
persistent identity: annotation2109416
display id: annotation2109416
version: 1
top level: BBa_K325905
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K325905/annotation2109416/range2109416/1

https://synbiohub.org/public/igem/BBa_K325905/annotation2109416/range2109416/1
type: Range
persistent identity: range2109416
display id: range2109416
version: 1
direction: direction/forward
top level: BBa_K325905
start: 5492
end: 6475
orientation: inline

BBa_K389413
type: ComponentDefinition
persistent identity: BBa K389413
display id: BBa_K389413
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: vir promoter + sensitivity tuner + luciferase
creator: Jonas Aretz
created: 2010-10-10T11:00:00Z
modified: 2015-05-08T01:12:19Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _517_
m user id: 0
owner id: 6670
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part combines a mutated virG response regulator from the VirA/G receptor system under the control of a constitutive promoter with a sensitivity tuner before a luciferase gene under the control of a vir promoter. The sensitivity tuner enhances a signal from a promoter so it enhances the readout luciferase.
mutable notes: sensitivity tuner to enhance the luciferase signal
mutable provenance: * luciferase from pGL4.10luc2 (Promega) * sensitivity tuner part, terminators and constitutive promoter from the partsregistry * ''virG'' was synthesized by Mr. Gene * ''vir'' promoter from Agrobacterium tumefaciens C58, TI-plasmid
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K389003
component: BBa_K389002
component: BBa_K389403
component: BBa_B0017
component: BBa_J23110
sequence annotation: BBa_J23110
sequence annotation: BBa_K389403
sequence annotation: BBa_K389002
sequence annotation: BBa_B0017
sequence annotation: BBa_K389003
sequence: https://synbiohub.org/public/igem/BBa_K389413_sequence/1

BBa_K389003
type: Component
persistent identity: component2300814
display id: component2300814
version: 1
top level: BBa_K389413
access: public
definition: https://synbiohub.org/public/igem/BBa_K389003/1

BBa_K389002
type: Component
persistent identity: component2300804
display id: component2300804
version: 1
top level: BBa_K389413
access: public
definition: https://synbiohub.org/public/igem/BBa_K389002/1

BBa_K389403
type: Component
persistent identity: component2300839
display id: component2300839
version: 1
top level: BBa_K389413
access: public
definition: BBa_K389403

BBa_B0017
type: Component
persistent identity: component2300809
display id: component2300809
version: 1
top level: BBa_K389413
access: public
definition: https://synbiohub.org/public/igem/BBa_B0017/1

BBa_J23110
type: Component
persistent identity: component2300800
display id: component2300800
version: 1
top level: BBa_K389413
access: public
definition: https://synbiohub.org/public/igem/BBa_J23110/1

BBa_J23110
type: SequenceAnnotation
persistent identity: annotation2300800
display id: annotation2300800
version: 1
top level: BBa_K389413
location: https://synbiohub.org/public/igem/BBa_K389413/annotation2300800/range2300800/1
component: BBa_J23110

https://synbiohub.org/public/igem/BBa_K389413/annotation2300800/range2300800/1
type: Range
persistent identity: range2300800
display id: range2300800
version: 1
direction: direction/reverse
top level: BBa_K389413
start: 1
end: 35
orientation: inline

BBa_K389403
type: SequenceAnnotation
persistent identity: annotation2300839
display id: annotation2300839
version: 1
top level: BBa_K389413
location: https://synbiohub.org/public/igem/BBa_K389413/annotation2300839/range2300839/1
component: BBa_K389403

https://synbiohub.org/public/igem/BBa_K389413/annotation2300839/range2300839/1
type: Range
persistent identity: range2300839
display id: range2300839
version: 1
direction: direction/reverse
top level: BBa_K389413
start: 1134
end: 4047
orientation: inline

BBa_K389002
type: SequenceAnnotation
persistent identity: annotation2300804
display id: annotation2300804
version: 1
top level: BBa_K389413
location: https://synbiohub.org/public/igem/BBa_K389413/annotation2300804/range2300804/1
component: BBa_K389002

https://synbiohub.org/public/igem/BBa_K389413/annotation2300804/range2300804/1
type: Range
persistent identity: range2300804
display id: range2300804
version: 1
direction: direction/reverse
top level: BBa_K389413
start: 44
end: 783
orientation: inline

BBa_B0017
type: SequenceAnnotation
persistent identity: annotation2300809
display id: annotation2300809
version: 1
top level: BBa_K389413
location: https://synbiohub.org/public/igem/BBa_K389413/annotation2300809/range2300809/1
component: BBa_B0017

https://synbiohub.org/public/igem/BBa_K389413/annotation2300809/range2300809/1
type: Range
persistent identity: range2300809
display id: range2300809
version: 1
direction: direction/reverse
top level: BBa_K389413
start: 792
end: 959
orientation: inline

BBa_K389003
type: SequenceAnnotation
persistent identity: annotation2300814
display id: annotation2300814
version: 1
top level: BBa_K389413
location: https://synbiohub.org/public/igem/BBa_K389413/annotation2300814/range2300814/1
component: BBa_K389003

https://synbiohub.org/public/igem/BBa_K389413/annotation2300814/range2300814/1
type: Range
persistent identity: range2300814
display id: range2300814
version: 1
direction: direction/reverse
top level: BBa_K389413
start: 968
end: 1125
orientation: inline

BBa_K1689004
type: ComponentDefinition
persistent identity: BBa K1689004
display id: BBa_K1689004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Coding sequence of Nluc398-FRB
creator: Xiang Li
created: 2015-09-15T11:00:00Z
modified: 2016-01-19T01:27:13Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2107_
m user id: 4206
owner id: 26464
owning group id: 9
status: status/Planning
bookmark: false
mutable description: including N terminal 398 amino acid domain, 2??linker, and FRB.
mutable notes: N terminal 416 amino acid domain and FRB are linked with 2??linker.
mutable provenance: E.coli
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: B0034
sequence annotation: start codon
sequence annotation: GGGGSGGGGS linker
sequence annotation: stop codon
sequence annotation: N398 terminal of firefly luciferase
sequence annotation: J23110
sequence annotation: FRB
sequence: https://synbiohub.org/public/igem/BBa_K1689004_sequence/1

B0034
type: SequenceAnnotation
persistent identity: annotation2470055
display id: annotation2470055
version: 1
top level: BBa_K1689004
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1689004/annotation2470055/range2470055/1

https://synbiohub.org/public/igem/BBa_K1689004/annotation2470055/range2470055/1
type: Range
persistent identity: range2470055
display id: range2470055
version: 1
direction: direction/forward
top level: BBa_K1689004
start: 36
end: 47
orientation: inline

start codon
type: SequenceAnnotation
persistent identity: annotation2470144
display id: annotation2470144
version: 1
top level: BBa_K1689004
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1689004/annotation2470144/range2470144/1

https://synbiohub.org/public/igem/BBa_K1689004/annotation2470144/range2470144/1
type: Range
persistent identity: range2470144
display id: range2470144
version: 1
direction: direction/forward
top level: BBa_K1689004
start: 48
end: 50
orientation: inline

GGGGSGGGGS linker
type: SequenceAnnotation
persistent identity: annotation2470076
display id: annotation2470076
version: 1
top level: BBa_K1689004
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1689004/annotation2470076/range2470076/1

https://synbiohub.org/public/igem/BBa_K1689004/annotation2470076/range2470076/1
type: Range
persistent identity: range2470076
display id: range2470076
version: 1
direction: direction/forward
top level: BBa_K1689004
start: 1242
end: 1271
orientation: inline

stop codon
type: SequenceAnnotation
persistent identity: annotation2470145
display id: annotation2470145
version: 1
top level: BBa_K1689004
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1689004/annotation2470145/range2470145/1

https://synbiohub.org/public/igem/BBa_K1689004/annotation2470145/range2470145/1
type: Range
persistent identity: range2470145
display id: range2470145
version: 1
direction: direction/forward
top level: BBa_K1689004
start: 1551
end: 1553
orientation: inline

N398 terminal of firefly luciferase
type: SequenceAnnotation
persistent identity: annotation2470059
display id: annotation2470059
version: 1
top level: BBa_K1689004
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1689004/annotation2470059/range2470059/1

https://synbiohub.org/public/igem/BBa_K1689004/annotation2470059/range2470059/1
type: Range
persistent identity: range2470059
display id: range2470059
version: 1
direction: direction/forward
top level: BBa_K1689004
start: 51
end: 1240
orientation: inline

J23110
type: SequenceAnnotation
persistent identity: annotation2470054
display id: annotation2470054
version: 1
top level: BBa_K1689004
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1689004/annotation2470054/range2470054/1

https://synbiohub.org/public/igem/BBa_K1689004/annotation2470054/range2470054/1
type: Range
persistent identity: range2470054
display id: range2470054
version: 1
direction: direction/forward
top level: BBa_K1689004
start: 1
end: 35
orientation: inline

FRB
type: SequenceAnnotation
persistent identity: annotation2470080
display id: annotation2470080
version: 1
top level: BBa_K1689004
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1689004/annotation2470080/range2470080/1

https://synbiohub.org/public/igem/BBa_K1689004/annotation2470080/range2470080/1
type: Range
persistent identity: range2470080
display id: range2470080
version: 1
direction: direction/forward
top level: BBa_K1689004
start: 1272
end: 1550
orientation: inline

BBa_K1486030
type: ComponentDefinition
persistent identity: BBa K1486030
display id: BBa_K1486030
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: rLucN + ADH1 terminator + Kanamycin resistance
creator: EPFL iGem team 2014
created: 2014-09-26T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: To edit
mutable notes: To edit
mutable provenance: To edit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K1486019
component: BBa_K1486024
component: BBa_K1486037
component: BBa_K1486025
sequence annotation: BBa_K1486037
sequence annotation: BBa_K1486025
sequence annotation: BBa_K1486024
sequence annotation: BBa_K1486019
sequence: https://synbiohub.org/public/igem/BBa_K1486030_sequence/1

BBa_K1486019
type: Component
persistent identity: component2417717
display id: component2417717
version: 1
top level: BBa_K1486030
access: public
definition: BBa_K1486019

BBa_K1486024
type: Component
persistent identity: component2417722
display id: component2417722
version: 1
top level: BBa_K1486030
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486024/1

BBa_K1486037
type: Component
persistent identity: component2417716
display id: component2417716
version: 1
top level: BBa_K1486030
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486037/1

BBa_K1486025
type: Component
persistent identity: component2417718
display id: component2417718
version: 1
top level: BBa_K1486030
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486025/1

BBa_K1486037
type: SequenceAnnotation
persistent identity: annotation2417716
display id: annotation2417716
version: 1
top level: BBa_K1486030
location: https://synbiohub.org/public/igem/BBa_K1486030/annotation2417716/range2417716/1
component: BBa_K1486037

https://synbiohub.org/public/igem/BBa_K1486030/annotation2417716/range2417716/1
type: Range
persistent identity: range2417716
display id: range2417716
version: 1
direction: direction/reverse
top level: BBa_K1486030
start: 1
end: 39
orientation: inline

BBa_K1486025
type: SequenceAnnotation
persistent identity: annotation2417718
display id: annotation2417718
version: 1
top level: BBa_K1486030
location: https://synbiohub.org/public/igem/BBa_K1486030/annotation2417718/range2417718/1
component: BBa_K1486025

https://synbiohub.org/public/igem/BBa_K1486030/annotation2417718/range2417718/1
type: Range
persistent identity: range2417718
display id: range2417718
version: 1
direction: direction/reverse
top level: BBa_K1486030
start: 370
end: 557
orientation: inline

BBa_K1486024
type: SequenceAnnotation
persistent identity: annotation2417722
display id: annotation2417722
version: 1
top level: BBa_K1486030
location: https://synbiohub.org/public/igem/BBa_K1486030/annotation2417722/range2417722/1
component: BBa_K1486024

https://synbiohub.org/public/igem/BBa_K1486030/annotation2417722/range2417722/1
type: Range
persistent identity: range2417722
display id: range2417722
version: 1
direction: direction/reverse
top level: BBa_K1486030
start: 558
end: 1914
orientation: inline

BBa_K1486019
type: SequenceAnnotation
persistent identity: annotation2417717
display id: annotation2417717
version: 1
top level: BBa_K1486030
location: https://synbiohub.org/public/igem/BBa_K1486030/annotation2417717/range2417717/1
component: BBa_K1486019

https://synbiohub.org/public/igem/BBa_K1486030/annotation2417717/range2417717/1
type: Range
persistent identity: range2417717
display id: range2417717
version: 1
direction: direction/reverse
top level: BBa_K1486030
start: 40
end: 369
orientation: inline

SP-Lipocal
type: ComponentDefinition
persistent identity: BBa K157039
display id: BBa_K157039
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: SP-Lipocalin-GGGSlinker-Tm-Splitluciferase1/2
creator: iGEM Team Freiburg 2008
created: 2008-10-25T11:00:00Z
modified: 2015-05-08T01:10:55Z
discontinued: false
dominant: false
experience: experience/None
group u list: _232_
m user id: 0
owner id: 1673
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: A composite part consisting of the signal peptide of Erbb1/EGF-receptor, scFV-antiNIP, GGGSflexlinker (extracellular part), the trans-membrane region of the Erbb1/EGF-receptor and the C-terminal part of the split fluorophor YFP with a fluolinker joined to its N-Terminus (intracellular part).
mutable notes: none
mutable provenance: Derived by cloning of the parts BBa_K157001, BBa_K157004, BBa_K157013, BBa_K157002 and the second half of split Luciferase For further information see the description of the parts mentioned above.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: Tm
sequence annotation: AgeI
sequence annotation: Splitluciferase1/2
sequence annotation: Scar (Thr, Gly)
sequence annotation: NgoMI
sequence annotation: SP
sequence annotation: Lipocalin
sequence annotation: GGGS-Linker
sequence annotation: SP-Lipocalin-GGGSlinker-Tm-Splitluciferase1/2
sequence: https://synbiohub.org/public/igem/BBa_K157039_sequence/1

Tm
type: SequenceAnnotation
persistent identity: annotation1993433
display id: annotation1993433
version: 1
top level: SP-Lipocal
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157039/annotation1993433/range1993433/1

https://synbiohub.org/public/igem/BBa_K157039/annotation1993433/range1993433/1
type: Range
persistent identity: range1993433
display id: range1993433
version: 1
direction: direction/forward
top level: SP-Lipocal
start: 667
end: 735
orientation: inline

AgeI
type: SequenceAnnotation
persistent identity: annotation1990304
display id: annotation1990304
version: 1
top level: SP-Lipocal
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157039/annotation1990304/range1990304/1

https://synbiohub.org/public/igem/BBa_K157039/annotation1990304/range1990304/1
type: Range
persistent identity: range1990304
display id: range1990304
version: 1
direction: direction/forward
top level: SP-Lipocal
start: 970
end: 975
orientation: inline

Splitluciferase1/2
type: SequenceAnnotation
persistent identity: annotation1993435
display id: annotation1993435
version: 1
top level: SP-Lipocal
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157039/annotation1993435/range1993435/1

https://synbiohub.org/public/igem/BBa_K157039/annotation1993435/range1993435/1
type: Range
persistent identity: range1993435
display id: range1993435
version: 1
direction: direction/forward
top level: SP-Lipocal
start: 742
end: 969
orientation: inline

Scar (Thr, Gly)
type: SequenceAnnotation
persistent identity: annotation1993430
display id: annotation1993430
version: 1
top level: SP-Lipocal
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157039/annotation1993430/range1993430/1

https://synbiohub.org/public/igem/BBa_K157039/annotation1993430/range1993430/1
type: Range
persistent identity: range1993430
display id: range1993430
version: 1
direction: direction/forward
top level: SP-Lipocal
start: 610
end: 615
orientation: inline

NgoMI
type: SequenceAnnotation
persistent identity: annotation1990303
display id: annotation1990303
version: 1
top level: SP-Lipocal
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157039/annotation1990303/range1990303/1

https://synbiohub.org/public/igem/BBa_K157039/annotation1990303/range1990303/1
type: Range
persistent identity: range1990303
display id: range1990303
version: 1
direction: direction/forward
top level: SP-Lipocal
start: 4
end: 9
orientation: inline

Scar (Thr, Gly)
type: SequenceAnnotation
persistent identity: annotation1993432
display id: annotation1993432
version: 1
top level: SP-Lipocal
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157039/annotation1993432/range1993432/1

https://synbiohub.org/public/igem/BBa_K157039/annotation1993432/range1993432/1
type: Range
persistent identity: range1993432
display id: range1993432
version: 1
direction: direction/forward
top level: SP-Lipocal
start: 661
end: 666
orientation: inline

SP
type: SequenceAnnotation
persistent identity: annotation1993427
display id: annotation1993427
version: 1
top level: SP-Lipocal
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157039/annotation1993427/range1993427/1

https://synbiohub.org/public/igem/BBa_K157039/annotation1993427/range1993427/1
type: Range
persistent identity: range1993427
display id: range1993427
version: 1
direction: direction/forward
top level: SP-Lipocal
start: 10
end: 81
orientation: inline

Lipocalin
type: SequenceAnnotation
persistent identity: annotation1993429
display id: annotation1993429
version: 1
top level: SP-Lipocal
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157039/annotation1993429/range1993429/1

https://synbiohub.org/public/igem/BBa_K157039/annotation1993429/range1993429/1
type: Range
persistent identity: range1993429
display id: range1993429
version: 1
direction: direction/forward
top level: SP-Lipocal
start: 88
end: 609
orientation: inline

GGGS-Linker
type: SequenceAnnotation
persistent identity: annotation1993431
display id: annotation1993431
version: 1
top level: SP-Lipocal
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157039/annotation1993431/range1993431/1

https://synbiohub.org/public/igem/BBa_K157039/annotation1993431/range1993431/1
type: Range
persistent identity: range1993431
display id: range1993431
version: 1
direction: direction/forward
top level: SP-Lipocal
start: 616
end: 660
orientation: inline

Scar (Thr, Gly)
type: SequenceAnnotation
persistent identity: annotation1993434
display id: annotation1993434
version: 1
top level: SP-Lipocal
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157039/annotation1993434/range1993434/1

https://synbiohub.org/public/igem/BBa_K157039/annotation1993434/range1993434/1
type: Range
persistent identity: range1993434
display id: range1993434
version: 1
direction: direction/forward
top level: SP-Lipocal
start: 736
end: 741
orientation: inline

SP-Lipocalin-GGGSlinker-Tm-Splitluciferase1/2
type: SequenceAnnotation
persistent identity: annotation1990305
display id: annotation1990305
version: 1
top level: SP-Lipocal
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K157039/annotation1990305/range1990305/1

https://synbiohub.org/public/igem/BBa_K157039/annotation1990305/range1990305/1
type: Range
persistent identity: range1990305
display id: range1990305
version: 1
direction: direction/forward
top level: SP-Lipocal
start: 1
end: 975
orientation: inline

Scar (Thr, Gly)
type: SequenceAnnotation
persistent identity: annotation1993428
display id: annotation1993428
version: 1
top level: SP-Lipocal
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157039/annotation1993428/range1993428/1

https://synbiohub.org/public/igem/BBa_K157039/annotation1993428/range1993428/1
type: Range
persistent identity: range1993428
display id: range1993428
version: 1
direction: direction/forward
top level: SP-Lipocal
start: 82
end: 87
orientation: inline

BBa_K389422
type: ComponentDefinition
persistent identity: BBa K389422
display id: BBa_K389422
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: VirA/G signaling system + sensitivity tuner + luciferase
creator: Jonas Aretz
created: 2010-10-10T11:00:00Z
modified: 2015-05-08T01:12:19Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _517_
m user id: 0
owner id: 6670
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part combines a complete VirA/G signaling system with a sensitivity tuner before a luciferase gene under the control of a ''vir'' promoter. The sensitivity tuner enhances a signal from a promoter so it enhances the readout luciferase.
mutable notes: sensitivity tuner to enhance the luciferase signal
mutable provenance: * luciferase from pGL4.10luc2 (Promega) * sensitivity tuner part, terminators and constitutive promoter from the partsregistry * ''virG'' was synthesized by Mr. Gene * ''virA'' and ''vir'' promoter from ''Agrobacterium tumefaciens'' C58, TI-plasmid
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K389402
component: BBa_K389017
sequence annotation: BBa_K389402
sequence annotation: BBa_K389017
sequence: https://synbiohub.org/public/igem/BBa_K389422_sequence/1

BBa_K389402
type: Component
persistent identity: component2313920
display id: component2313920
version: 1
top level: BBa_K389422
access: public
definition: BBa_K389402

BBa_K389017
type: Component
persistent identity: component2313896
display id: component2313896
version: 1
top level: BBa_K389422
access: public
definition: https://synbiohub.org/public/igem/BBa_K389017/1

BBa_K389402
type: SequenceAnnotation
persistent identity: annotation2313920
display id: annotation2313920
version: 1
top level: BBa_K389422
location: https://synbiohub.org/public/igem/BBa_K389422/annotation2313920/range2313920/1
component: BBa_K389402

https://synbiohub.org/public/igem/BBa_K389422/annotation2313920/range2313920/1
type: Range
persistent identity: range2313920
display id: range2313920
version: 1
direction: direction/reverse
top level: BBa_K389422
start: 3708
end: 6594
orientation: inline

BBa_K389017
type: SequenceAnnotation
persistent identity: annotation2313896
display id: annotation2313896
version: 1
top level: BBa_K389422
location: https://synbiohub.org/public/igem/BBa_K389422/annotation2313896/range2313896/1
component: BBa_K389017

https://synbiohub.org/public/igem/BBa_K389422/annotation2313896/range2313896/1
type: Range
persistent identity: range2313896
display id: range2313896
version: 1
direction: direction/reverse
top level: BBa_K389422
start: 1
end: 3699
orientation: inline

fLuc
type: ComponentDefinition
persistent identity: BBa K518000
display id: BBa_K518000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS + firefly luciferase + d.terminator
creator: Masato Ohgishi
created: 2011-09-14T11:00:00Z
modified: 2015-05-08T01:12:33Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _683_
m user id: 0
owner id: 8305
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Luciferase from firyfly (Photinus pyralis). Firefly luciferase emits light by the oxidation of D-luciferin, its substrate. Luciferase can be used as a measuring tool when combined with other cis-elements (regions regulating the expression of genes on the same DNA molecule; promoters and other protein-binding domains), because of its highly quantitative performance.
mutable notes: RBS (medium) B0032, firefly luciferase CDS I712019, double-terminator B0014
mutable provenance: From BioBrick parts.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_I712019
component: BBa_B0014
component: BBa_B0032
sequence annotation: BBa_B0014
sequence annotation: BBa_B0032
sequence annotation: BBa_I712019
sequence: https://synbiohub.org/public/igem/BBa_K518000_sequence/1

BBa_I712019
type: Component
persistent identity: component2128620
display id: component2128620
version: 1
top level: fLuc
access: public
definition: BBa_I712019

BBa_B0014
type: Component
persistent identity: component2128627
display id: component2128627
version: 1
top level: fLuc
access: public
definition: https://synbiohub.org/public/igem/BBa_B0014/1

BBa_B0032
type: Component
persistent identity: component2128618
display id: component2128618
version: 1
top level: fLuc
access: public
definition: https://synbiohub.org/public/igem/BBa_B0032/1

BBa_B0014
type: SequenceAnnotation
persistent identity: annotation2128627
display id: annotation2128627
version: 1
top level: fLuc
location: https://synbiohub.org/public/igem/BBa_K518000/annotation2128627/range2128627/1
component: BBa_B0014

https://synbiohub.org/public/igem/BBa_K518000/annotation2128627/range2128627/1
type: Range
persistent identity: range2128627
display id: range2128627
version: 1
direction: direction/reverse
top level: fLuc
start: 1681
end: 1775
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation2128618
display id: annotation2128618
version: 1
top level: fLuc
location: https://synbiohub.org/public/igem/BBa_K518000/annotation2128618/range2128618/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_K518000/annotation2128618/range2128618/1
type: Range
persistent identity: range2128618
display id: range2128618
version: 1
direction: direction/forward
top level: fLuc
start: 1
end: 13
orientation: inline

BBa_I712019
type: SequenceAnnotation
persistent identity: annotation2128620
display id: annotation2128620
version: 1
top level: fLuc
location: https://synbiohub.org/public/igem/BBa_K518000/annotation2128620/range2128620/1
component: BBa_I712019

https://synbiohub.org/public/igem/BBa_K518000/annotation2128620/range2128620/1
type: Range
persistent identity: range2128620
display id: range2128620
version: 1
direction: direction/reverse
top level: fLuc
start: 20
end: 1672
orientation: inline

BBa_K1486020
type: ComponentDefinition
persistent identity: BBa K1486020
display id: BBa_K1486020
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: rLuc[2]
creator: EPFL iGem team 2014
created: 2014-09-14T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is the second part of the split Renilla luciferase. To be used with rLuc[1].
mutable notes: to edit
mutable provenance: to edit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence: https://synbiohub.org/public/igem/BBa_K1486020_sequence/1

BBa_K216016
type: ComponentDefinition
persistent identity: BBa K216016
display id: BBa_K216016
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PyeaR with bacterial luciferase
creator: Edinburgh iGEM 2009
created: 2009-10-20T11:00:00Z
modified: 2015-05-08T01:11:30Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _317_
m user id: 0
owner id: 837
owning group id: 163
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: This is nitrate- and nitrite-responsive promoter PyeaR attached to bacterial luciferase luxAB of ''Xenorhabdus (Photorhabdus) luminescens''. It produces light in the presence of nitrate or nitrite when supplied with a long chain aldehyde (eg, decanal) as substrate.
mutable notes: No special considerations.
mutable provenance: Made by joing BBa_K216005 to BBa_K216008
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_K216005
component: BBa_K216008
sequence annotation: BBa_K216008
sequence annotation: BBa_K216005
sequence: https://synbiohub.org/public/igem/BBa_K216016_sequence/1

BBa_K216005
type: Component
persistent identity: component2058194
display id: component2058194
version: 1
top level: BBa_K216016
access: public
definition: https://synbiohub.org/public/igem/BBa_K216005/1

BBa_K216008
type: Component
persistent identity: component2058203
display id: component2058203
version: 1
top level: BBa_K216016
access: public
definition: BBa_K216008

BBa_K216008
type: SequenceAnnotation
persistent identity: annotation2058203
display id: annotation2058203
version: 1
top level: BBa_K216016
location: https://synbiohub.org/public/igem/BBa_K216016/annotation2058203/range2058203/1
component: BBa_K216008

https://synbiohub.org/public/igem/BBa_K216016/annotation2058203/range2058203/1
type: Range
persistent identity: range2058203
display id: range2058203
version: 1
direction: direction/reverse
top level: BBa_K216016
start: 109
end: 2218
orientation: inline

BBa_K216005
type: SequenceAnnotation
persistent identity: annotation2058194
display id: annotation2058194
version: 1
top level: BBa_K216016
location: https://synbiohub.org/public/igem/BBa_K216016/annotation2058194/range2058194/1
component: BBa_K216005

https://synbiohub.org/public/igem/BBa_K216016/annotation2058194/range2058194/1
type: Range
persistent identity: range2058194
display id: range2058194
version: 1
direction: direction/reverse
top level: BBa_K216016
start: 1
end: 100
orientation: inline

BBa_K1680009
type: ComponentDefinition
persistent identity: BBa K1680009
display id: BBa_K1680009
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: NanoLuc Luciferase
creator: Katharina Sporbeck
created: 2015-09-16T11:00:00Z
modified: 2015-10-27T01:37:07Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2098_
m user id: 15693
owner id: 26460
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Protein coding region for the NanoLuc(tm) Luziferase (designed by the Promega Corporation, free for research use), Codon optimized for yeast. The Nanoluc is smaller than other luziferase proteins and yields equal or stronger luminescence readings.
mutable notes: Part is shipped in RFC10.
mutable provenance: gBlock designed by Team Tuebingen, sequence by Promega
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: NanoLuc
sequence: https://synbiohub.org/public/igem/BBa_K1680009_sequence/1

NanoLuc
type: SequenceAnnotation
persistent identity: annotation2477413
display id: annotation2477413
version: 1
top level: BBa_K1680009
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1680009/annotation2477413/range2477413/1

https://synbiohub.org/public/igem/BBa_K1680009/annotation2477413/range2477413/1
type: Range
persistent identity: range2477413
display id: range2477413
version: 1
direction: direction/forward
top level: BBa_K1680009
start: 1
end: 516
orientation: inline

BBa_K1486021
type: ComponentDefinition
persistent identity: BBa K1486021
display id: BBa_K1486021
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Arabinose promoter + rLuc[1] + rLuc[2]
creator: EPFL iGem team 2014
created: 2014-09-14T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This part contains both fragments of the split renilla luciferase, each of them preceded by an Elowitz RBS.
mutable notes: to edit
mutable provenance: to edit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0034
component: BBa_K1486020
component: BBa_I0500
component: BBa_K1486019
sequence annotation: BBa_B0034
sequence annotation: BBa_K1486019
sequence annotation: BBa_I0500
sequence annotation: BBa_K1486020
sequence: https://synbiohub.org/public/igem/BBa_K1486021_sequence/1

BBa_B0034
type: Component
persistent identity: component2383466
display id: component2383466
version: 1
top level: BBa_K1486021
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K1486020
type: Component
persistent identity: component2383467
display id: component2383467
version: 1
top level: BBa_K1486021
access: public
definition: BBa_K1486020

BBa_I0500
type: Component
persistent identity: component2383461
display id: component2383461
version: 1
top level: BBa_K1486021
access: public
definition: https://synbiohub.org/public/igem/BBa_I0500/1

BBa_K1486019
type: Component
persistent identity: component2383464
display id: component2383464
version: 1
top level: BBa_K1486021
access: public
definition: BBa_K1486019

BBa_B0034
type: Component
persistent identity: component2383463
display id: component2383463
version: 1
top level: BBa_K1486021
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2383466
display id: annotation2383466
version: 1
top level: BBa_K1486021
location: https://synbiohub.org/public/igem/BBa_K1486021/annotation2383466/range2383466/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1486021/annotation2383466/range2383466/1
type: Range
persistent identity: range2383466
display id: range2383466
version: 1
direction: direction/reverse
top level: BBa_K1486021
start: 1553
end: 1564
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2383463
display id: annotation2383463
version: 1
top level: BBa_K1486021
location: https://synbiohub.org/public/igem/BBa_K1486021/annotation2383463/range2383463/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1486021/annotation2383463/range2383463/1
type: Range
persistent identity: range2383463
display id: range2383463
version: 1
direction: direction/reverse
top level: BBa_K1486021
start: 1211
end: 1222
orientation: inline

BBa_K1486019
type: SequenceAnnotation
persistent identity: annotation2383464
display id: annotation2383464
version: 1
top level: BBa_K1486021
location: https://synbiohub.org/public/igem/BBa_K1486021/annotation2383464/range2383464/1
component: BBa_K1486019

https://synbiohub.org/public/igem/BBa_K1486021/annotation2383464/range2383464/1
type: Range
persistent identity: range2383464
display id: range2383464
version: 1
direction: direction/reverse
top level: BBa_K1486021
start: 1223
end: 1552
orientation: inline

BBa_I0500
type: SequenceAnnotation
persistent identity: annotation2383461
display id: annotation2383461
version: 1
top level: BBa_K1486021
location: https://synbiohub.org/public/igem/BBa_K1486021/annotation2383461/range2383461/1
component: BBa_I0500

https://synbiohub.org/public/igem/BBa_K1486021/annotation2383461/range2383461/1
type: Range
persistent identity: range2383461
display id: range2383461
version: 1
direction: direction/reverse
top level: BBa_K1486021
start: 1
end: 1210
orientation: inline

BBa_K1486020
type: SequenceAnnotation
persistent identity: annotation2383467
display id: annotation2383467
version: 1
top level: BBa_K1486021
location: https://synbiohub.org/public/igem/BBa_K1486021/annotation2383467/range2383467/1
component: BBa_K1486020

https://synbiohub.org/public/igem/BBa_K1486021/annotation2383467/range2383467/1
type: Range
persistent identity: range2383467
display id: range2383467
version: 1
direction: direction/reverse
top level: BBa_K1486021
start: 1565
end: 2170
orientation: inline

luc
type: ComponentDefinition
persistent identity: BBa K389004
display id: BBa_K389004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Luciferase from pGL4.10[luc2]
creator: Jonas Aretz
created: 2010-08-09T11:00:00Z
modified: 2015-05-08T01:12:19Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _517_
m user id: 0
owner id: 6670
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: A luciferase from Promega's pGL4.10luc2 vector.
mutable notes: sequence of this vector is already published by Promega
mutable provenance: pGL4.10luc2
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: Stop codon
sequence annotation: Luciferase
sequence: https://synbiohub.org/public/igem/BBa_K389004_sequence/1

Stop codon
type: SequenceAnnotation
persistent identity: annotation2078364
display id: annotation2078364
version: 1
top level: luc
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K389004/annotation2078364/range2078364/1

https://synbiohub.org/public/igem/BBa_K389004/annotation2078364/range2078364/1
type: Range
persistent identity: range2078364
display id: range2078364
version: 1
direction: direction/forward
top level: luc
start: 1654
end: 1656
orientation: inline

Luciferase
type: SequenceAnnotation
persistent identity: annotation2077057
display id: annotation2077057
version: 1
top level: luc
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K389004/annotation2077057/range2077057/1

https://synbiohub.org/public/igem/BBa_K389004/annotation2077057/range2077057/1
type: Range
persistent identity: range2077057
display id: range2077057
version: 1
direction: direction/forward
top level: luc
start: 1
end: 1653
orientation: inline

BBa_K2023007
type: ComponentDefinition
persistent identity: BBa K2023007
display id: BBa_K2023007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Gluc coding device with Pr
creator: C??lia Chenebault
created: 2016-10-13T11:00:00Z
modified: 2016-10-20T04:13:44Z
discontinued: false
dominant: false
group u list: _2490_
m user id: 30356
owner id: 30230
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Gluc coding device with Pr use as the positive control in gaussia luciferase assay
mutable notes: blabla
mutable provenance: IDT synthesis
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_I723018
component: BBa_B0034
component: BBa_K2023009
component: BBa_B0014
sequence annotation: BBa_B0034
sequence annotation: BBa_K2023009
sequence annotation: BBa_B0014
sequence annotation: BBa_I723018
sequence: https://synbiohub.org/public/igem/BBa_K2023007_sequence/1

BBa_I723018
type: Component
persistent identity: component2522653
display id: component2522653
version: 1
top level: BBa_K2023007
access: public
definition: https://synbiohub.org/public/igem/BBa_I723018/1

BBa_B0034
type: Component
persistent identity: component2522655
display id: component2522655
version: 1
top level: BBa_K2023007
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K2023009
type: Component
persistent identity: component2522657
display id: component2522657
version: 1
top level: BBa_K2023007
access: public
definition: BBa_K2023009

BBa_B0014
type: Component
persistent identity: component2522664
display id: component2522664
version: 1
top level: BBa_K2023007
access: public
definition: https://synbiohub.org/public/igem/BBa_B0014/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2522655
display id: annotation2522655
version: 1
top level: BBa_K2023007
location: https://synbiohub.org/public/igem/BBa_K2023007/annotation2522655/range2522655/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K2023007/annotation2522655/range2522655/1
type: Range
persistent identity: range2522655
display id: range2522655
version: 1
direction: direction/reverse
top level: BBa_K2023007
start: 419
end: 430
orientation: inline

BBa_K2023009
type: SequenceAnnotation
persistent identity: annotation2522657
display id: annotation2522657
version: 1
top level: BBa_K2023007
location: https://synbiohub.org/public/igem/BBa_K2023007/annotation2522657/range2522657/1
component: BBa_K2023009

https://synbiohub.org/public/igem/BBa_K2023007/annotation2522657/range2522657/1
type: Range
persistent identity: range2522657
display id: range2522657
version: 1
direction: direction/reverse
top level: BBa_K2023007
start: 437
end: 997
orientation: inline

BBa_B0014
type: SequenceAnnotation
persistent identity: annotation2522664
display id: annotation2522664
version: 1
top level: BBa_K2023007
location: https://synbiohub.org/public/igem/BBa_K2023007/annotation2522664/range2522664/1
component: BBa_B0014

https://synbiohub.org/public/igem/BBa_K2023007/annotation2522664/range2522664/1
type: Range
persistent identity: range2522664
display id: range2522664
version: 1
direction: direction/reverse
top level: BBa_K2023007
start: 1006
end: 1100
orientation: inline

BBa_I723018
type: SequenceAnnotation
persistent identity: annotation2522653
display id: annotation2522653
version: 1
top level: BBa_K2023007
location: https://synbiohub.org/public/igem/BBa_K2023007/annotation2522653/range2522653/1
component: BBa_I723018

https://synbiohub.org/public/igem/BBa_K2023007/annotation2522653/range2522653/1
type: Range
persistent identity: range2522653
display id: range2522653
version: 1
direction: direction/reverse
top level: BBa_K2023007
start: 1
end: 410
orientation: inline

BBa_K1486018
type: ComponentDefinition
persistent identity: BBa K1486018
display id: BBa_K1486018
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Arabinose promoter + fLuc[1] + fLuc[2]
creator: EPFL iGem team 2014
created: 2014-09-14T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part contains both fragments of the split firefly luciferase, each of them preceded by an Elowitz RBS.
mutable notes: to edit (start/stop codons)
mutable provenance: to edit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K1486017
component: BBa_B0034
component: BBa_I0500
component: BBa_K1486016
sequence annotation: BBa_B0034
sequence annotation: BBa_K1486017
sequence annotation: BBa_I0500
sequence annotation: BBa_K1486016
sequence: https://synbiohub.org/public/igem/BBa_K1486018_sequence/1

BBa_K1486017
type: Component
persistent identity: component2383447
display id: component2383447
version: 1
top level: BBa_K1486018
access: public
definition: BBa_K1486017

BBa_B0034
type: Component
persistent identity: component2383446
display id: component2383446
version: 1
top level: BBa_K1486018
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0034
type: Component
persistent identity: component2383443
display id: component2383443
version: 1
top level: BBa_K1486018
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_I0500
type: Component
persistent identity: component2383441
display id: component2383441
version: 1
top level: BBa_K1486018
access: public
definition: https://synbiohub.org/public/igem/BBa_I0500/1

BBa_K1486016
type: Component
persistent identity: component2383444
display id: component2383444
version: 1
top level: BBa_K1486018
access: public
definition: BBa_K1486016

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2383446
display id: annotation2383446
version: 1
top level: BBa_K1486018
location: https://synbiohub.org/public/igem/BBa_K1486018/annotation2383446/range2383446/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1486018/annotation2383446/range2383446/1
type: Range
persistent identity: range2383446
display id: range2383446
version: 1
direction: direction/reverse
top level: BBa_K1486018
start: 1697
end: 1708
orientation: inline

BBa_K1486017
type: SequenceAnnotation
persistent identity: annotation2383447
display id: annotation2383447
version: 1
top level: BBa_K1486018
location: https://synbiohub.org/public/igem/BBa_K1486018/annotation2383447/range2383447/1
component: BBa_K1486017

https://synbiohub.org/public/igem/BBa_K1486018/annotation2383447/range2383447/1
type: Range
persistent identity: range2383447
display id: range2383447
version: 1
direction: direction/reverse
top level: BBa_K1486018
start: 1709
end: 2956
orientation: inline

BBa_I0500
type: SequenceAnnotation
persistent identity: annotation2383441
display id: annotation2383441
version: 1
top level: BBa_K1486018
location: https://synbiohub.org/public/igem/BBa_K1486018/annotation2383441/range2383441/1
component: BBa_I0500

https://synbiohub.org/public/igem/BBa_K1486018/annotation2383441/range2383441/1
type: Range
persistent identity: range2383441
display id: range2383441
version: 1
direction: direction/reverse
top level: BBa_K1486018
start: 1
end: 1210
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2383443
display id: annotation2383443
version: 1
top level: BBa_K1486018
location: https://synbiohub.org/public/igem/BBa_K1486018/annotation2383443/range2383443/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1486018/annotation2383443/range2383443/1
type: Range
persistent identity: range2383443
display id: range2383443
version: 1
direction: direction/reverse
top level: BBa_K1486018
start: 1211
end: 1222
orientation: inline

BBa_K1486016
type: SequenceAnnotation
persistent identity: annotation2383444
display id: annotation2383444
version: 1
top level: BBa_K1486018
location: https://synbiohub.org/public/igem/BBa_K1486018/annotation2383444/range2383444/1
component: BBa_K1486016

https://synbiohub.org/public/igem/BBa_K1486018/annotation2383444/range2383444/1
type: Range
persistent identity: range2383444
display id: range2383444
version: 1
direction: direction/reverse
top level: BBa_K1486018
start: 1223
end: 1696
orientation: inline

BBa_K1732003
type: ComponentDefinition
persistent identity: BBa K1732003
display id: BBa_K1732003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: GLUCCO-His
creator: Donna Lee
created: 2015-09-06T11:00:00Z
modified: 2016-01-19T01:23:56Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2154_
m user id: 4206
owner id: 27195
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Gaussia Luciferase, also known as Gluc, is a bioluminescent luciferase protein that was isolated from Gaussia princeps. When reacted with the optimal concentration of the substrate coelenterazine, a green light will luminescence.
mutable notes: This gene has been codon optimized for E.coli.
mutable provenance: Designed by Cheryl Telmer of the Bruchez Lab in Carnegie Mellon University.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: GFPCO
sequence annotation: 6X-His
sequence: https://synbiohub.org/public/igem/BBa_K1732003_sequence/1

GFPCO
type: SequenceAnnotation
persistent identity: annotation2475212
display id: annotation2475212
version: 1
top level: BBa_K1732003
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1732003/annotation2475212/range2475212/1

https://synbiohub.org/public/igem/BBa_K1732003/annotation2475212/range2475212/1
type: Range
persistent identity: range2475212
display id: range2475212
version: 1
direction: direction/forward
top level: BBa_K1732003
start: 1
end: 503
orientation: inline

6X-His
type: SequenceAnnotation
persistent identity: annotation2475213
display id: annotation2475213
version: 1
top level: BBa_K1732003
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1732003/annotation2475213/range2475213/1

https://synbiohub.org/public/igem/BBa_K1732003/annotation2475213/range2475213/1
type: Range
persistent identity: range2475213
display id: range2475213
version: 1
direction: direction/forward
top level: BBa_K1732003
start: 504
end: 522
orientation: inline

SP-AntiNIP
type: ComponentDefinition
persistent identity: BBa K157031
display id: BBa_K157031
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: SP-AntiNIP-GGGSlinker-Tm-Splitluciferase1/2
creator: iGEM Team Freiburg 2008
created: 2008-10-25T11:00:00Z
modified: 2015-05-08T01:10:55Z
discontinued: false
dominant: true
experience: experience/None
group u list: _232_
m user id: 0
owner id: 1673
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Part consists of the signal peptide of Erbb1/EGF-receptor, scFV-antiNIP, GGGSflexlinker (extracellular part), the trans-membrane region of the Erbb1/EGF-receptor and the C-terminal part of the split fluorophor YFP with a fluolinker joined to its N-Terminus (intracellular part).
mutable notes: none
mutable provenance: Derived by cloning of the parts BBa_K157001, BBa_K157003, BBa_K157013, BBa_K157002 and Part 2 of Split- Luciferase For further information see the description of the parts mentioned above.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: Scar (Thr, Gly)
sequence annotation: NgoMI
sequence annotation: SP-AntiNIP-GGGSlinker-Tm-Splitluciferase1/2
sequence annotation: SP
sequence annotation: Sc-Fv_anti-NIP
sequence annotation: Splitluciferase1/2
sequence annotation: Tm
sequence annotation: AgeI
sequence annotation: GGGS-Linker
sequence: https://synbiohub.org/public/igem/BBa_K157031_sequence/1

Scar (Thr, Gly)
type: SequenceAnnotation
persistent identity: annotation1993319
display id: annotation1993319
version: 1
top level: SP-AntiNIP
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157031/annotation1993319/range1993319/1

https://synbiohub.org/public/igem/BBa_K157031/annotation1993319/range1993319/1
type: Range
persistent identity: range1993319
display id: range1993319
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 82
end: 87
orientation: inline

Scar (Thr, Gly)
type: SequenceAnnotation
persistent identity: annotation1993325
display id: annotation1993325
version: 1
top level: SP-AntiNIP
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157031/annotation1993325/range1993325/1

https://synbiohub.org/public/igem/BBa_K157031/annotation1993325/range1993325/1
type: Range
persistent identity: range1993325
display id: range1993325
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 949
end: 954
orientation: inline

NgoMI
type: SequenceAnnotation
persistent identity: annotation1990266
display id: annotation1990266
version: 1
top level: SP-AntiNIP
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157031/annotation1990266/range1990266/1

https://synbiohub.org/public/igem/BBa_K157031/annotation1990266/range1990266/1
type: Range
persistent identity: range1990266
display id: range1990266
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 4
end: 9
orientation: inline

Scar (Thr, Gly)
type: SequenceAnnotation
persistent identity: annotation1993321
display id: annotation1993321
version: 1
top level: SP-AntiNIP
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157031/annotation1993321/range1993321/1

https://synbiohub.org/public/igem/BBa_K157031/annotation1993321/range1993321/1
type: Range
persistent identity: range1993321
display id: range1993321
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 823
end: 828
orientation: inline

SP-AntiNIP-GGGSlinker-Tm-Splitluciferase1/2
type: SequenceAnnotation
persistent identity: annotation1990268
display id: annotation1990268
version: 1
top level: SP-AntiNIP
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K157031/annotation1990268/range1990268/1

https://synbiohub.org/public/igem/BBa_K157031/annotation1990268/range1990268/1
type: Range
persistent identity: range1990268
display id: range1990268
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 1
end: 1236
orientation: inline

SP
type: SequenceAnnotation
persistent identity: annotation1993318
display id: annotation1993318
version: 1
top level: SP-AntiNIP
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157031/annotation1993318/range1993318/1

https://synbiohub.org/public/igem/BBa_K157031/annotation1993318/range1993318/1
type: Range
persistent identity: range1993318
display id: range1993318
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 10
end: 81
orientation: inline

Sc-Fv_anti-NIP
type: SequenceAnnotation
persistent identity: annotation1993320
display id: annotation1993320
version: 1
top level: SP-AntiNIP
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157031/annotation1993320/range1993320/1

https://synbiohub.org/public/igem/BBa_K157031/annotation1993320/range1993320/1
type: Range
persistent identity: range1993320
display id: range1993320
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 88
end: 822
orientation: inline

Splitluciferase1/2
type: SequenceAnnotation
persistent identity: annotation1993326
display id: annotation1993326
version: 1
top level: SP-AntiNIP
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157031/annotation1993326/range1993326/1

https://synbiohub.org/public/igem/BBa_K157031/annotation1993326/range1993326/1
type: Range
persistent identity: range1993326
display id: range1993326
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 955
end: 1230
orientation: inline

Tm
type: SequenceAnnotation
persistent identity: annotation1993324
display id: annotation1993324
version: 1
top level: SP-AntiNIP
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157031/annotation1993324/range1993324/1

https://synbiohub.org/public/igem/BBa_K157031/annotation1993324/range1993324/1
type: Range
persistent identity: range1993324
display id: range1993324
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 880
end: 948
orientation: inline

AgeI
type: SequenceAnnotation
persistent identity: annotation1990267
display id: annotation1990267
version: 1
top level: SP-AntiNIP
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157031/annotation1990267/range1990267/1

https://synbiohub.org/public/igem/BBa_K157031/annotation1990267/range1990267/1
type: Range
persistent identity: range1990267
display id: range1990267
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 1231
end: 1236
orientation: inline

Scar (Thr, Gly)
type: SequenceAnnotation
persistent identity: annotation1993323
display id: annotation1993323
version: 1
top level: SP-AntiNIP
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157031/annotation1993323/range1993323/1

https://synbiohub.org/public/igem/BBa_K157031/annotation1993323/range1993323/1
type: Range
persistent identity: range1993323
display id: range1993323
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 874
end: 879
orientation: inline

GGGS-Linker
type: SequenceAnnotation
persistent identity: annotation1993322
display id: annotation1993322
version: 1
top level: SP-AntiNIP
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157031/annotation1993322/range1993322/1

https://synbiohub.org/public/igem/BBa_K157031/annotation1993322/range1993322/1
type: Range
persistent identity: range1993322
display id: range1993322
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 829
end: 873
orientation: inline

BBa_K1486043
type: ComponentDefinition
persistent identity: BBa K1486043
display id: BBa_K1486043
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Leucine Zipper + split rLuc
creator: EPFL iGem team 2014
created: 2014-10-01T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: To edit. Pense ?? rajouter les linkers, je ne sais pas s'ils sont de igem ou s'il faut ajouter la s??quence ?? la registry. Check pour tous tes biobricks et dis-moi, please.
mutable notes: To edit
mutable provenance: To edit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K1486019
component: BBa_K1486042
component: BBa_K1486020
component: BBa_B0034
component: BBa_I0500
sequence annotation: BBa_K1486020
sequence annotation: BBa_K1486042
sequence annotation: BBa_K1486019
sequence annotation: BBa_B0034
sequence annotation: BBa_I0500
sequence: https://synbiohub.org/public/igem/BBa_K1486043_sequence/1

BBa_K1486019
type: Component
persistent identity: component2392174
display id: component2392174
version: 1
top level: BBa_K1486043
access: public
definition: BBa_K1486019

BBa_K1486042
type: Component
persistent identity: component2392177
display id: component2392177
version: 1
top level: BBa_K1486043
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486042/1

BBa_K1486020
type: Component
persistent identity: component2392178
display id: component2392178
version: 1
top level: BBa_K1486043
access: public
definition: BBa_K1486020

BBa_K1486042
type: Component
persistent identity: component2392173
display id: component2392173
version: 1
top level: BBa_K1486043
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486042/1

BBa_B0034
type: Component
persistent identity: component2392172
display id: component2392172
version: 1
top level: BBa_K1486043
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0034
type: Component
persistent identity: component2392176
display id: component2392176
version: 1
top level: BBa_K1486043
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_I0500
type: Component
persistent identity: component2392170
display id: component2392170
version: 1
top level: BBa_K1486043
access: public
definition: https://synbiohub.org/public/igem/BBa_I0500/1

BBa_K1486020
type: SequenceAnnotation
persistent identity: annotation2392178
display id: annotation2392178
version: 1
top level: BBa_K1486043
location: https://synbiohub.org/public/igem/BBa_K1486043/annotation2392178/range2392178/1
component: BBa_K1486020

https://synbiohub.org/public/igem/BBa_K1486043/annotation2392178/range2392178/1
type: Range
persistent identity: range2392178
display id: range2392178
version: 1
direction: direction/reverse
top level: BBa_K1486043
start: 1865
end: 2470
orientation: inline

BBa_K1486042
type: SequenceAnnotation
persistent identity: annotation2392173
display id: annotation2392173
version: 1
top level: BBa_K1486043
location: https://synbiohub.org/public/igem/BBa_K1486043/annotation2392173/range2392173/1
component: BBa_K1486042

https://synbiohub.org/public/igem/BBa_K1486043/annotation2392173/range2392173/1
type: Range
persistent identity: range2392173
display id: range2392173
version: 1
direction: direction/reverse
top level: BBa_K1486043
start: 1223
end: 1372
orientation: inline

BBa_K1486019
type: SequenceAnnotation
persistent identity: annotation2392174
display id: annotation2392174
version: 1
top level: BBa_K1486043
location: https://synbiohub.org/public/igem/BBa_K1486043/annotation2392174/range2392174/1
component: BBa_K1486019

https://synbiohub.org/public/igem/BBa_K1486043/annotation2392174/range2392174/1
type: Range
persistent identity: range2392174
display id: range2392174
version: 1
direction: direction/reverse
top level: BBa_K1486043
start: 1373
end: 1702
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2392172
display id: annotation2392172
version: 1
top level: BBa_K1486043
location: https://synbiohub.org/public/igem/BBa_K1486043/annotation2392172/range2392172/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1486043/annotation2392172/range2392172/1
type: Range
persistent identity: range2392172
display id: range2392172
version: 1
direction: direction/reverse
top level: BBa_K1486043
start: 1211
end: 1222
orientation: inline

BBa_I0500
type: SequenceAnnotation
persistent identity: annotation2392170
display id: annotation2392170
version: 1
top level: BBa_K1486043
location: https://synbiohub.org/public/igem/BBa_K1486043/annotation2392170/range2392170/1
component: BBa_I0500

https://synbiohub.org/public/igem/BBa_K1486043/annotation2392170/range2392170/1
type: Range
persistent identity: range2392170
display id: range2392170
version: 1
direction: direction/reverse
top level: BBa_K1486043
start: 1
end: 1210
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2392176
display id: annotation2392176
version: 1
top level: BBa_K1486043
location: https://synbiohub.org/public/igem/BBa_K1486043/annotation2392176/range2392176/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1486043/annotation2392176/range2392176/1
type: Range
persistent identity: range2392176
display id: range2392176
version: 1
direction: direction/reverse
top level: BBa_K1486043
start: 1703
end: 1714
orientation: inline

BBa_K1486042
type: SequenceAnnotation
persistent identity: annotation2392177
display id: annotation2392177
version: 1
top level: BBa_K1486043
location: https://synbiohub.org/public/igem/BBa_K1486043/annotation2392177/range2392177/1
component: BBa_K1486042

https://synbiohub.org/public/igem/BBa_K1486043/annotation2392177/range2392177/1
type: Range
persistent identity: range2392177
display id: range2392177
version: 1
direction: direction/reverse
top level: BBa_K1486043
start: 1715
end: 1864
orientation: inline

SbpA | Luc
type: ComponentDefinition
persistent identity: BBa K525411
display id: BBa_K525411
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Fusion Protein of S-Layer SbpA and Firefly-Luciferase
creator: Anna Drong
created: 2011-09-12T11:00:00Z
modified: 2015-05-08T01:12:34Z
discontinued: false
dominant: false
experience: experience/None
group u list: _690_
m user id: 0
owner id: 8543
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: PT7 + RBS + SpbA || Luc
mutable notes: Assembled
mutable provenance: BBa_K525403, BBa_K525999
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: lac operator
sequence annotation: K525999
sequence annotation: RBS
sequence annotation: T7 promoter
sequence annotation: NgoMIV
sequence annotation: B0034
sequence annotation: TAA
sequence annotation: Luciferase
sequence annotation: ATG
sequence annotation: Freiburg scar
sequence annotation: AgeI
sequence annotation: SbpA domain
sequence annotation: K525401
sequence: https://synbiohub.org/public/igem/BBa_K525411_sequence/1

lac operator
type: SequenceAnnotation
persistent identity: annotation2161268
display id: annotation2161268
version: 1
top level: SbpA | Luc
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K525411/annotation2161268/range2161268/1

https://synbiohub.org/public/igem/BBa_K525411/annotation2161268/range2161268/1
type: Range
persistent identity: range2161268
display id: range2161268
version: 1
direction: direction/forward
top level: SbpA | Luc
start: 20
end: 44
orientation: inline

K525999
type: SequenceAnnotation
persistent identity: annotation2161276
display id: annotation2161276
version: 1
top level: SbpA | Luc
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K525411/annotation2161276/range2161276/1

https://synbiohub.org/public/igem/BBa_K525411/annotation2161276/range2161276/1
type: Range
persistent identity: range2161276
display id: range2161276
version: 1
direction: direction/forward
top level: SbpA | Luc
start: 3199
end: 4854
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2161270
display id: annotation2161270
version: 1
top level: SbpA | Luc
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K525411/annotation2161270/range2161270/1

https://synbiohub.org/public/igem/BBa_K525411/annotation2161270/range2161270/1
type: Range
persistent identity: range2161270
display id: range2161270
version: 1
direction: direction/forward
top level: SbpA | Luc
start: 56
end: 59
orientation: inline

T7 promoter
type: SequenceAnnotation
persistent identity: annotation2161267
display id: annotation2161267
version: 1
top level: SbpA | Luc
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K525411/annotation2161267/range2161267/1

https://synbiohub.org/public/igem/BBa_K525411/annotation2161267/range2161267/1
type: Range
persistent identity: range2161267
display id: range2161267
version: 1
direction: direction/forward
top level: SbpA | Luc
start: 1
end: 20
orientation: inline

NgoMIV
type: SequenceAnnotation
persistent identity: annotation2161279
display id: annotation2161279
version: 1
top level: SbpA | Luc
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K525411/annotation2161279/range2161279/1

https://synbiohub.org/public/igem/BBa_K525411/annotation2161279/range2161279/1
type: Range
persistent identity: range2161279
display id: range2161279
version: 1
direction: direction/forward
top level: SbpA | Luc
start: 76
end: 81
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation2161269
display id: annotation2161269
version: 1
top level: SbpA | Luc
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K525411/annotation2161269/range2161269/1

https://synbiohub.org/public/igem/BBa_K525411/annotation2161269/range2161269/1
type: Range
persistent identity: range2161269
display id: range2161269
version: 1
direction: direction/forward
top level: SbpA | Luc
start: 53
end: 64
orientation: inline

TAA
type: SequenceAnnotation
persistent identity: annotation2161278
display id: annotation2161278
version: 1
top level: SbpA | Luc
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K525411/annotation2161278/range2161278/1

https://synbiohub.org/public/igem/BBa_K525411/annotation2161278/range2161278/1
type: Range
persistent identity: range2161278
display id: range2161278
version: 1
direction: direction/forward
top level: SbpA | Luc
start: 4852
end: 4854
orientation: inline

Luciferase
type: SequenceAnnotation
persistent identity: annotation2161275
display id: annotation2161275
version: 1
top level: SbpA | Luc
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K525411/annotation2161275/range2161275/1

https://synbiohub.org/public/igem/BBa_K525411/annotation2161275/range2161275/1
type: Range
persistent identity: range2161275
display id: range2161275
version: 1
direction: direction/forward
top level: SbpA | Luc
start: 3199
end: 4854
orientation: inline

ATG
type: SequenceAnnotation
persistent identity: annotation2161273
display id: annotation2161273
version: 1
top level: SbpA | Luc
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K525411/annotation2161273/range2161273/1

https://synbiohub.org/public/igem/BBa_K525411/annotation2161273/range2161273/1
type: Range
persistent identity: range2161273
display id: range2161273
version: 1
direction: direction/forward
top level: SbpA | Luc
start: 73
end: 75
orientation: inline

Freiburg scar
type: SequenceAnnotation
persistent identity: annotation2161274
display id: annotation2161274
version: 1
top level: SbpA | Luc
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K525411/annotation2161274/range2161274/1

https://synbiohub.org/public/igem/BBa_K525411/annotation2161274/range2161274/1
type: Range
persistent identity: range2161274
display id: range2161274
version: 1
direction: direction/forward
top level: SbpA | Luc
start: 3193
end: 3198
orientation: inline

AgeI
type: SequenceAnnotation
persistent identity: annotation2161277
display id: annotation2161277
version: 1
top level: SbpA | Luc
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K525411/annotation2161277/range2161277/1

https://synbiohub.org/public/igem/BBa_K525411/annotation2161277/range2161277/1
type: Range
persistent identity: range2161277
display id: range2161277
version: 1
direction: direction/forward
top level: SbpA | Luc
start: 4846
end: 4851
orientation: inline

SbpA domain
type: SequenceAnnotation
persistent identity: annotation2161272
display id: annotation2161272
version: 1
top level: SbpA | Luc
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K525411/annotation2161272/range2161272/1

https://synbiohub.org/public/igem/BBa_K525411/annotation2161272/range2161272/1
type: Range
persistent identity: range2161272
display id: range2161272
version: 1
direction: direction/forward
top level: SbpA | Luc
start: 73
end: 3192
orientation: inline

K525401
type: SequenceAnnotation
persistent identity: annotation2161271
display id: annotation2161271
version: 1
top level: SbpA | Luc
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K525411/annotation2161271/range2161271/1

https://synbiohub.org/public/igem/BBa_K525411/annotation2161271/range2161271/1
type: Range
persistent identity: range2161271
display id: range2161271
version: 1
direction: direction/forward
top level: SbpA | Luc
start: 73
end: 3192
orientation: inline

BBa_K389412
type: ComponentDefinition
persistent identity: BBa K389412
display id: BBa_K389412
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: vir promoter + sensitivity tuner + luciferase
creator: Jonas Aretz
created: 2010-10-10T11:00:00Z
modified: 2015-05-08T01:12:19Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _517_
m user id: 0
owner id: 6670
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part combines a mutated virG response regulator from the VirA/G receptor system under the control of a constitutive promoter with a sensitivity tuner before a luciferase gene under the control of a vir promoter. The sensitivity tuner enhances a signal from a promoter so it enhances the readout luciferase.
mutable notes: sensitivity tuner to enhance the luciferase signal
mutable provenance: * luciferase from pGL4.10luc2 (Promega) * sensitivity tuner part, terminators and constitutive promoter from the partsregistry * ''virG'' was synthesized by Mr. Gene * ''vir'' promoter from ''Agrobacterium tumefaciens'' C58, TI-plasmid
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K389002
component: BBa_K389003
component: BBa_J23110
component: BBa_B0017
component: BBa_K389402
sequence annotation: BBa_K389003
sequence annotation: BBa_K389402
sequence annotation: BBa_B0017
sequence annotation: BBa_J23110
sequence annotation: BBa_K389002
sequence: https://synbiohub.org/public/igem/BBa_K389412_sequence/1

BBa_K389002
type: Component
persistent identity: component2300765
display id: component2300765
version: 1
top level: BBa_K389412
access: public
definition: https://synbiohub.org/public/igem/BBa_K389002/1

BBa_K389003
type: Component
persistent identity: component2300775
display id: component2300775
version: 1
top level: BBa_K389412
access: public
definition: https://synbiohub.org/public/igem/BBa_K389003/1

BBa_J23110
type: Component
persistent identity: component2300761
display id: component2300761
version: 1
top level: BBa_K389412
access: public
definition: https://synbiohub.org/public/igem/BBa_J23110/1

BBa_B0017
type: Component
persistent identity: component2300770
display id: component2300770
version: 1
top level: BBa_K389412
access: public
definition: https://synbiohub.org/public/igem/BBa_B0017/1

BBa_K389402
type: Component
persistent identity: component2300799
display id: component2300799
version: 1
top level: BBa_K389412
access: public
definition: BBa_K389402

BBa_K389003
type: SequenceAnnotation
persistent identity: annotation2300775
display id: annotation2300775
version: 1
top level: BBa_K389412
location: https://synbiohub.org/public/igem/BBa_K389412/annotation2300775/range2300775/1
component: BBa_K389003

https://synbiohub.org/public/igem/BBa_K389412/annotation2300775/range2300775/1
type: Range
persistent identity: range2300775
display id: range2300775
version: 1
direction: direction/reverse
top level: BBa_K389412
start: 968
end: 1125
orientation: inline

BBa_K389402
type: SequenceAnnotation
persistent identity: annotation2300799
display id: annotation2300799
version: 1
top level: BBa_K389412
location: https://synbiohub.org/public/igem/BBa_K389412/annotation2300799/range2300799/1
component: BBa_K389402

https://synbiohub.org/public/igem/BBa_K389412/annotation2300799/range2300799/1
type: Range
persistent identity: range2300799
display id: range2300799
version: 1
direction: direction/reverse
top level: BBa_K389412
start: 1134
end: 4020
orientation: inline

BBa_B0017
type: SequenceAnnotation
persistent identity: annotation2300770
display id: annotation2300770
version: 1
top level: BBa_K389412
location: https://synbiohub.org/public/igem/BBa_K389412/annotation2300770/range2300770/1
component: BBa_B0017

https://synbiohub.org/public/igem/BBa_K389412/annotation2300770/range2300770/1
type: Range
persistent identity: range2300770
display id: range2300770
version: 1
direction: direction/reverse
top level: BBa_K389412
start: 792
end: 959
orientation: inline

BBa_J23110
type: SequenceAnnotation
persistent identity: annotation2300761
display id: annotation2300761
version: 1
top level: BBa_K389412
location: https://synbiohub.org/public/igem/BBa_K389412/annotation2300761/range2300761/1
component: BBa_J23110

https://synbiohub.org/public/igem/BBa_K389412/annotation2300761/range2300761/1
type: Range
persistent identity: range2300761
display id: range2300761
version: 1
direction: direction/reverse
top level: BBa_K389412
start: 1
end: 35
orientation: inline

BBa_K389002
type: SequenceAnnotation
persistent identity: annotation2300765
display id: annotation2300765
version: 1
top level: BBa_K389412
location: https://synbiohub.org/public/igem/BBa_K389412/annotation2300765/range2300765/1
component: BBa_K389002

https://synbiohub.org/public/igem/BBa_K389412/annotation2300765/range2300765/1
type: Range
persistent identity: range2300765
display id: range2300765
version: 1
direction: direction/reverse
top level: BBa_K389412
start: 44
end: 783
orientation: inline

Tm-Splitlu
type: ComponentDefinition
persistent identity: BBa K157021
display id: BBa_K157021
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Tm-Splitluciferase2/2
creator: iGEM Team Freiburg 2008
created: 2008-10-25T11:00:00Z
modified: 2015-05-08T01:10:54Z
discontinued: false
dominant: false
experience: experience/None
group u list: _232_
m user id: 0
owner id: 1673
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Second part of Split-Luciferase fused to the trans-membrane region of the Erbb1/EGF-receptor.
mutable notes: none
mutable provenance: Derived by cloning of the parts BBa_K157002 and second part of Split-Luciferase. For further information see the description of the parts mentioned above.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: NgoMI
sequence annotation: Tm
sequence annotation: Split-Luciferase2
sequence annotation: Tm-Split-Luciferase2/2
sequence annotation: AgeI
sequence annotation: Scar (Thr, Gly)
sequence: https://synbiohub.org/public/igem/BBa_K157021_sequence/1

NgoMI
type: SequenceAnnotation
persistent identity: annotation1991542
display id: annotation1991542
version: 1
top level: Tm-Splitlu
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157021/annotation1991542/range1991542/1

https://synbiohub.org/public/igem/BBa_K157021/annotation1991542/range1991542/1
type: Range
persistent identity: range1991542
display id: range1991542
version: 1
direction: direction/forward
top level: Tm-Splitlu
start: 4
end: 9
orientation: inline

Tm
type: SequenceAnnotation
persistent identity: annotation1991543
display id: annotation1991543
version: 1
top level: Tm-Splitlu
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157021/annotation1991543/range1991543/1

https://synbiohub.org/public/igem/BBa_K157021/annotation1991543/range1991543/1
type: Range
persistent identity: range1991543
display id: range1991543
version: 1
direction: direction/forward
top level: Tm-Splitlu
start: 10
end: 78
orientation: inline

Split-Luciferase2
type: SequenceAnnotation
persistent identity: annotation1991545
display id: annotation1991545
version: 1
top level: Tm-Splitlu
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157021/annotation1991545/range1991545/1

https://synbiohub.org/public/igem/BBa_K157021/annotation1991545/range1991545/1
type: Range
persistent identity: range1991545
display id: range1991545
version: 1
direction: direction/forward
top level: Tm-Splitlu
start: 85
end: 360
orientation: inline

Tm-Split-Luciferase2/2
type: SequenceAnnotation
persistent identity: annotation1991541
display id: annotation1991541
version: 1
top level: Tm-Splitlu
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K157021/annotation1991541/range1991541/1

https://synbiohub.org/public/igem/BBa_K157021/annotation1991541/range1991541/1
type: Range
persistent identity: range1991541
display id: range1991541
version: 1
direction: direction/forward
top level: Tm-Splitlu
start: 1
end: 366
orientation: inline

AgeI
type: SequenceAnnotation
persistent identity: annotation1991546
display id: annotation1991546
version: 1
top level: Tm-Splitlu
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157021/annotation1991546/range1991546/1

https://synbiohub.org/public/igem/BBa_K157021/annotation1991546/range1991546/1
type: Range
persistent identity: range1991546
display id: range1991546
version: 1
direction: direction/forward
top level: Tm-Splitlu
start: 361
end: 366
orientation: inline

Scar (Thr, Gly)
type: SequenceAnnotation
persistent identity: annotation1991544
display id: annotation1991544
version: 1
top level: Tm-Splitlu
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157021/annotation1991544/range1991544/1

https://synbiohub.org/public/igem/BBa_K157021/annotation1991544/range1991544/1
type: Range
persistent identity: range1991544
display id: range1991544
version: 1
direction: direction/forward
top level: Tm-Splitlu
start: 79
end: 84
orientation: inline

BBa_K2023003
type: ComponentDefinition
persistent identity: BBa K2023003
display id: BBa_K2023003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Gluc coding device with Pu
creator: C??lia Chenebault
created: 2016-10-13T11:00:00Z
modified: 2016-10-20T03:23:39Z
discontinued: false
dominant: false
group u list: _2490_
m user id: 30356
owner id: 30230
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Gluc coding device with Pu
mutable notes: blabla
mutable provenance: IDT synthesis
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_K2023009
component: BBa_B0014
component: BBa_I723020
component: BBa_B0034
sequence annotation: BBa_B0014
sequence annotation: BBa_I723020
sequence annotation: BBa_B0034
sequence annotation: BBa_K2023009
sequence: https://synbiohub.org/public/igem/BBa_K2023003_sequence/1

BBa_K2023009
type: Component
persistent identity: component2518688
display id: component2518688
version: 1
top level: BBa_K2023003
access: public
definition: BBa_K2023009

BBa_B0014
type: Component
persistent identity: component2518695
display id: component2518695
version: 1
top level: BBa_K2023003
access: public
definition: https://synbiohub.org/public/igem/BBa_B0014/1

BBa_I723020
type: Component
persistent identity: component2518684
display id: component2518684
version: 1
top level: BBa_K2023003
access: public
definition: https://synbiohub.org/public/igem/BBa_I723020/1

BBa_B0034
type: Component
persistent identity: component2518686
display id: component2518686
version: 1
top level: BBa_K2023003
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0014
type: SequenceAnnotation
persistent identity: annotation2518695
display id: annotation2518695
version: 1
top level: BBa_K2023003
location: https://synbiohub.org/public/igem/BBa_K2023003/annotation2518695/range2518695/1
component: BBa_B0014

https://synbiohub.org/public/igem/BBa_K2023003/annotation2518695/range2518695/1
type: Range
persistent identity: range2518695
display id: range2518695
version: 1
direction: direction/reverse
top level: BBa_K2023003
start: 916
end: 1010
orientation: inline

BBa_I723020
type: SequenceAnnotation
persistent identity: annotation2518684
display id: annotation2518684
version: 1
top level: BBa_K2023003
location: https://synbiohub.org/public/igem/BBa_K2023003/annotation2518684/range2518684/1
component: BBa_I723020

https://synbiohub.org/public/igem/BBa_K2023003/annotation2518684/range2518684/1
type: Range
persistent identity: range2518684
display id: range2518684
version: 1
direction: direction/reverse
top level: BBa_K2023003
start: 1
end: 320
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2518686
display id: annotation2518686
version: 1
top level: BBa_K2023003
location: https://synbiohub.org/public/igem/BBa_K2023003/annotation2518686/range2518686/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K2023003/annotation2518686/range2518686/1
type: Range
persistent identity: range2518686
display id: range2518686
version: 1
direction: direction/reverse
top level: BBa_K2023003
start: 329
end: 340
orientation: inline

BBa_K2023009
type: SequenceAnnotation
persistent identity: annotation2518688
display id: annotation2518688
version: 1
top level: BBa_K2023003
location: https://synbiohub.org/public/igem/BBa_K2023003/annotation2518688/range2518688/1
component: BBa_K2023009

https://synbiohub.org/public/igem/BBa_K2023003/annotation2518688/range2518688/1
type: Range
persistent identity: range2518688
display id: range2518688
version: 1
direction: direction/reverse
top level: BBa_K2023003
start: 347
end: 907
orientation: inline

BBa_J52008
type: ComponentDefinition
persistent identity: BBa J52008
display id: BBa_J52008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: luciferase: luciferin 2-monooxygenase from Renilla reniformis (EC 1.13.12.5; SwissProt:: P27652)
creator: Monika Ciglic
created: 2006-10-13T11:00:00Z
modified: 2015-08-31T03:54:04Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _80_
m user id: 0
owner id: 800
owning group id: 80
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: Rluc is a protein called Renilla`s luciferase and it emits light when adding the right substrate. That is why it can bi used as a reported to track other proteins or to monitor the activity of a promotor.
mutable notes: Rluc is cloned in BioBrick vector pSB1AK3.
mutable provenance: -
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: Rluc
sequence: https://synbiohub.org/public/igem/BBa_J52008_sequence/1

Rluc
type: SequenceAnnotation
persistent identity: annotation1902845
display id: annotation1902845
version: 1
top level: BBa_J52008
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J52008/annotation1902845/range1902845/1

https://synbiohub.org/public/igem/BBa_J52008/annotation1902845/range1902845/1
type: Range
persistent identity: range1902845
display id: range1902845
version: 1
direction: direction/forward
top level: BBa_J52008
start: 1
end: 936
orientation: inline

Rluc
type: SequenceAnnotation
persistent identity: annotation1902833
display id: annotation1902833
version: 1
top level: BBa_J52008
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_J52008/annotation1902833/range1902833/1

https://synbiohub.org/public/igem/BBa_J52008/annotation1902833/range1902833/1
type: Range
persistent identity: range1902833
display id: range1902833
version: 1
direction: direction/forward
top level: BBa_J52008
start: 1
end: 936
orientation: inline

BBa_K1761005
type: ComponentDefinition
persistent identity: BBa K1761005
display id: BBa_K1761005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LargeBit Split Luciferase
creator: Laura van Smeden
created: 2015-09-16T11:00:00Z
modified: 2016-01-19T01:26:53Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2183_
m user id: 4206
owner id: 25572
owning group id: 9
status: status/Planning
bookmark: false
mutable description: LargeBit is the big part of a Split Luciferase. Its molecular weight is 18 kDa. This construct on its own has no function since LargeBit on itself has no luminescence activity. For luminescence activity, the SmallBit of the Split Luciferace is needed.
mutable notes: The given sequence from the company is used
mutable provenance: The company Promega
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316

BBa_K1616025
type: ComponentDefinition
persistent identity: BBa K1616025
display id: BBa_K1616025
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: NanoLuc
creator: Marwa Zerhouni
created: 2015-09-18T11:00:00Z
modified: 2016-01-21T02:24:08Z
discontinued: false
dominant: false
group u list: _2033_
m user id: 4206
owner id: 24639
owning group id: 9
status: status/Planning
bookmark: false
mutable description: NanoLuc (Nluc)is a novel engineered luciferase coupled with a novel coelenterazine analogue: Furimazine. When both are coupled, we obtain a much brighter luminescence than Fluc or Rluc and it is known to work exceptionally well as a reporter. NanoLuc is a 19.1 kDa enzyme which is an ATP independent luciferase providing a glow-type luminescence.
mutable notes: We have checked for all illegal restriction sites.
mutable provenance: Kyle Hooper, Ph.D, Applications of a smaller, brighter, more versatile luciferase:NanoLuc??? Luciferase Technology, 2012
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1616025_sequence/1

BBa_K389421
type: ComponentDefinition
persistent identity: BBa K389421
display id: BBa_K389421
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: VirA/G signaling system + sensitivity tuner + luciferase
creator: Jonas Aretz
created: 2010-10-10T11:00:00Z
modified: 2015-05-08T01:12:19Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _517_
m user id: 0
owner id: 6670
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part combines a complete VirA/G signaling system with a sensitivity tuner before a luciferase gene under the control of a vir promoter. The sensitivity tuner enhances a signal from a promoter so it enhances the readout luciferase.
mutable notes: sensitivity tuner to enhance the luciferase signal
mutable provenance: * luciferase from pGL4.10luc2 (Promega) * sensitivity tuner part, terminators and constitutive promoter from the partsregistry * ''virG'' was synthesized by Mr. Gene * ''virA'' and ''vir'' promoter from Agrobacterium tumefaciens C58, TI-plasmid
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K389017
component: BBa_K389401
sequence annotation: BBa_K389401
sequence annotation: BBa_K389017
sequence: https://synbiohub.org/public/igem/BBa_K389421_sequence/1

BBa_K389017
type: Component
persistent identity: component2313848
display id: component2313848
version: 1
top level: BBa_K389421
access: public
definition: https://synbiohub.org/public/igem/BBa_K389017/1

BBa_K389401
type: Component
persistent identity: component2313872
display id: component2313872
version: 1
top level: BBa_K389421
access: public
definition: BBa_K389401

BBa_K389401
type: SequenceAnnotation
persistent identity: annotation2313872
display id: annotation2313872
version: 1
top level: BBa_K389421
location: https://synbiohub.org/public/igem/BBa_K389421/annotation2313872/range2313872/1
component: BBa_K389401

https://synbiohub.org/public/igem/BBa_K389421/annotation2313872/range2313872/1
type: Range
persistent identity: range2313872
display id: range2313872
version: 1
direction: direction/reverse
top level: BBa_K389421
start: 3708
end: 6594
orientation: inline

BBa_K389017
type: SequenceAnnotation
persistent identity: annotation2313848
display id: annotation2313848
version: 1
top level: BBa_K389421
location: https://synbiohub.org/public/igem/BBa_K389421/annotation2313848/range2313848/1
component: BBa_K389017

https://synbiohub.org/public/igem/BBa_K389421/annotation2313848/range2313848/1
type: Range
persistent identity: range2313848
display id: range2313848
version: 1
direction: direction/reverse
top level: BBa_K389421
start: 1
end: 3699
orientation: inline

BBa_K2023009
type: ComponentDefinition
persistent identity: BBa K2023009
display id: BBa_K2023009
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Gaussia luciferase coding sequence optimized for use in E.coli DH5-Alpha
creator: C??lia Chenebault
created: 2016-10-13T11:00:00Z
modified: 2016-10-20T04:19:03Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2490_
m user id: 30356
owner id: 30230
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Gaussia luciferase coding sequence optimized for use in E.coli
mutable notes: Consideration of the use in E.coli
mutable provenance: IDT synthesis, extraction from part 3
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: GLuc
sequence: https://synbiohub.org/public/igem/BBa_K2023009_sequence/1

GLuc
type: SequenceAnnotation
persistent identity: annotation2518419
display id: annotation2518419
version: 1
top level: BBa_K2023009
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K2023009/annotation2518419/range2518419/1

https://synbiohub.org/public/igem/BBa_K2023009/annotation2518419/range2518419/1
type: Range
persistent identity: range2518419
display id: range2518419
version: 1
direction: direction/forward
top level: BBa_K2023009
start: 1
end: 561
orientation: inline

BBa_K325210
type: ComponentDefinition
persistent identity: BBa K325210
display id: BBa_K325210
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Red Firefly Luciferase and LRE L. Cruciata (E. coli optimised)
creator: Hannah Copley and Theo Sanderson
created: 2010-09-14T11:00:00Z
modified: 2015-05-08T01:12:02Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _446_
m user id: 0
owner id: 7466
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This part is contains the coding sequence for a mutant light-emitting enzyme (luciferase) and luciferin regenerating enzyme (LRE) from the Japanese firefly, Luciola cruciata. It will make bacteria emit light at a reddish wavelength when placed under a promoter, provided the media is supplemented with luciferin. The luciferin regenerating enzyme will convert oxyluciferin into CHBT. CHBT is converted to more luciferin in the presence of D-cysteine.
mutable notes: Codon optimisation, making compatible with BioBrick assembly format.
mutable provenance: This was codon-optimised for E.coli and syntesised by DNA 2.0.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: Red luciferase (286) (L. cruciata)
sequence annotation: double stop codon
sequence annotation: rbs
sequence annotation: G to A: Ser286Asn
sequence annotation: Luciferin regenerating enzyme (L. cruciata)
sequence annotation: BamH1 site
sequence: https://synbiohub.org/public/igem/BBa_K325210_sequence/1

Red luciferase (286) (L. cruciata)
type: SequenceAnnotation
persistent identity: annotation2080154
display id: annotation2080154
version: 1
top level: BBa_K325210
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K325210/annotation2080154/range2080154/1

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type: Range
persistent identity: range2080154
display id: range2080154
version: 1
direction: direction/forward
top level: BBa_K325210
start: 974
end: 2617
orientation: inline

double stop codon
type: SequenceAnnotation
persistent identity: annotation2080151
display id: annotation2080151
version: 1
top level: BBa_K325210
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K325210/annotation2080151/range2080151/1

https://synbiohub.org/public/igem/BBa_K325210/annotation2080151/range2080151/1
type: Range
persistent identity: range2080151
display id: range2080151
version: 1
direction: direction/forward
top level: BBa_K325210
start: 945
end: 950
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation2080153
display id: annotation2080153
version: 1
top level: BBa_K325210
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K325210/annotation2080153/range2080153/1

https://synbiohub.org/public/igem/BBa_K325210/annotation2080153/range2080153/1
type: Range
persistent identity: range2080153
display id: range2080153
version: 1
direction: direction/forward
top level: BBa_K325210
start: 957
end: 973
orientation: inline

G to A: Ser286Asn
type: SequenceAnnotation
persistent identity: annotation2103099
display id: annotation2103099
version: 1
top level: BBa_K325210
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K325210/annotation2103099/range2103099/1

https://synbiohub.org/public/igem/BBa_K325210/annotation2103099/range2103099/1
type: Range
persistent identity: range2103099
display id: range2103099
version: 1
direction: direction/forward
top level: BBa_K325210
start: 1830
end: 1830
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation2080149
display id: annotation2080149
version: 1
top level: BBa_K325210
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K325210/annotation2080149/range2080149/1

https://synbiohub.org/public/igem/BBa_K325210/annotation2080149/range2080149/1
type: Range
persistent identity: range2080149
display id: range2080149
version: 1
direction: direction/forward
top level: BBa_K325210
start: 1
end: 17
orientation: inline

Luciferin regenerating enzyme (L. cruciata)
type: SequenceAnnotation
persistent identity: annotation2080150
display id: annotation2080150
version: 1
top level: BBa_K325210
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K325210/annotation2080150/range2080150/1

https://synbiohub.org/public/igem/BBa_K325210/annotation2080150/range2080150/1
type: Range
persistent identity: range2080150
display id: range2080150
version: 1
direction: direction/forward
top level: BBa_K325210
start: 18
end: 944
orientation: inline

BamH1 site
type: SequenceAnnotation
persistent identity: annotation2080152
display id: annotation2080152
version: 1
top level: BBa_K325210
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K325210/annotation2080152/range2080152/1

https://synbiohub.org/public/igem/BBa_K325210/annotation2080152/range2080152/1
type: Range
persistent identity: range2080152
display id: range2080152
version: 1
direction: direction/forward
top level: BBa_K325210
start: 951
end: 956
orientation: inline

double stop codon
type: SequenceAnnotation
persistent identity: annotation2080155
display id: annotation2080155
version: 1
top level: BBa_K325210
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K325210/annotation2080155/range2080155/1

https://synbiohub.org/public/igem/BBa_K325210/annotation2080155/range2080155/1
type: Range
persistent identity: range2080155
display id: range2080155
version: 1
direction: direction/forward
top level: BBa_K325210
start: 2618
end: 2623
orientation: inline

BBa_K389401
type: ComponentDefinition
persistent identity: BBa K389401
display id: BBa_K389401
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Sensitivity tuner + luciferase
creator: Jonas Aretz
created: 2010-10-10T11:00:00Z
modified: 2015-05-08T01:12:19Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _517_
m user id: 0
owner id: 6670
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This is a modified <partinfo>I746370</partinfo> sensitivity tuner. Instead of a GFP reporter, this one has a firefly luciferase (from Promega's pGL4.10luc2 vector) as one reporter gene. The arabinose promoter has been removed from the original part so now it is possible to assemble this part behind any promoter to get an enhanced luciferase signal.
mutable notes: arabinose promoter removed, GFP exchanged with luciferase compared to the original part <partinfo>I746370</partinfo>
mutable provenance: * luciferase from pGL4.10luc2 (Promega) * the rest from the partsregistry
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_B0015
component: BBa_B0034
component: BBa_K389004
component: BBa_I746360
component: BBa_I746350
component: BBa_E1010
sequence annotation: BBa_I746350
sequence annotation: BBa_E1010
sequence annotation: BBa_B0034
sequence annotation: BBa_K389004
sequence annotation: BBa_I746360
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_K389401_sequence/1

BBa_B0015
type: Component
persistent identity: component2273136
display id: component2273136
version: 1
top level: BBa_K389401
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_B0034
type: Component
persistent identity: component2273126
display id: component2273126
version: 1
top level: BBa_K389401
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K389004
type: Component
persistent identity: component2273143
display id: component2273143
version: 1
top level: BBa_K389401
access: public
definition: luc

BBa_I746360
type: Component
persistent identity: component2273138
display id: component2273138
version: 1
top level: BBa_K389401
access: public
definition: https://synbiohub.org/public/igem/BBa_I746360/1

BBa_B0034
type: Component
persistent identity: component2273140
display id: component2273140
version: 1
top level: BBa_K389401
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_I746350
type: Component
persistent identity: component2273124
display id: component2273124
version: 1
top level: BBa_K389401
access: public
definition: https://synbiohub.org/public/igem/BBa_I746350/1

BBa_E1010
type: Component
persistent identity: component2273129
display id: component2273129
version: 1
top level: BBa_K389401
access: public
definition: https://synbiohub.org/public/igem/BBa_E1010/1

BBa_I746350
type: SequenceAnnotation
persistent identity: annotation2273124
display id: annotation2273124
version: 1
top level: BBa_K389401
location: https://synbiohub.org/public/igem/BBa_K389401/annotation2273124/range2273124/1
component: BBa_I746350

https://synbiohub.org/public/igem/BBa_K389401/annotation2273124/range2273124/1
type: Range
persistent identity: range2273124
display id: range2273124
version: 1
direction: direction/reverse
top level: BBa_K389401
start: 1
end: 237
orientation: inline

BBa_E1010
type: SequenceAnnotation
persistent identity: annotation2273129
display id: annotation2273129
version: 1
top level: BBa_K389401
location: https://synbiohub.org/public/igem/BBa_K389401/annotation2273129/range2273129/1
component: BBa_E1010

https://synbiohub.org/public/igem/BBa_K389401/annotation2273129/range2273129/1
type: Range
persistent identity: range2273129
display id: range2273129
version: 1
direction: direction/reverse
top level: BBa_K389401
start: 264
end: 969
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2273126
display id: annotation2273126
version: 1
top level: BBa_K389401
location: https://synbiohub.org/public/igem/BBa_K389401/annotation2273126/range2273126/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K389401/annotation2273126/range2273126/1
type: Range
persistent identity: range2273126
display id: range2273126
version: 1
direction: direction/reverse
top level: BBa_K389401
start: 246
end: 257
orientation: inline

BBa_K389004
type: SequenceAnnotation
persistent identity: annotation2273143
display id: annotation2273143
version: 1
top level: BBa_K389401
location: https://synbiohub.org/public/igem/BBa_K389401/annotation2273143/range2273143/1
component: BBa_K389004

https://synbiohub.org/public/igem/BBa_K389401/annotation2273143/range2273143/1
type: Range
persistent identity: range2273143
display id: range2273143
version: 1
direction: direction/reverse
top level: BBa_K389401
start: 1232
end: 2887
orientation: inline

BBa_I746360
type: SequenceAnnotation
persistent identity: annotation2273138
display id: annotation2273138
version: 1
top level: BBa_K389401
location: https://synbiohub.org/public/igem/BBa_K389401/annotation2273138/range2273138/1
component: BBa_I746360

https://synbiohub.org/public/igem/BBa_K389401/annotation2273138/range2273138/1
type: Range
persistent identity: range2273138
display id: range2273138
version: 1
direction: direction/reverse
top level: BBa_K389401
start: 1115
end: 1205
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2273140
display id: annotation2273140
version: 1
top level: BBa_K389401
location: https://synbiohub.org/public/igem/BBa_K389401/annotation2273140/range2273140/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K389401/annotation2273140/range2273140/1
type: Range
persistent identity: range2273140
display id: range2273140
version: 1
direction: direction/reverse
top level: BBa_K389401
start: 1214
end: 1225
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2273136
display id: annotation2273136
version: 1
top level: BBa_K389401
location: https://synbiohub.org/public/igem/BBa_K389401/annotation2273136/range2273136/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K389401/annotation2273136/range2273136/1
type: Range
persistent identity: range2273136
display id: range2273136
version: 1
direction: direction/reverse
top level: BBa_K389401
start: 978
end: 1106
orientation: inline

BBa_K1486017
type: ComponentDefinition
persistent identity: BBa K1486017
display id: BBa_K1486017
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: fLuc[2]
creator: EPFL iGem team 2014
created: 2014-09-14T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is the second part of the split Firefly luciferase. To be used with fLuc[1].
mutable notes: to edit
mutable provenance: to edit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence: https://synbiohub.org/public/igem/BBa_K1486017_sequence/1

BBa_K389012
type: ComponentDefinition
persistent identity: BBa K389012
display id: BBa_K389012
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: VirA reporter system luc
creator: Jonas Aretz
created: 2010-09-10T11:00:00Z
modified: 2015-05-08T01:12:19Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _517_
m user id: 0
owner id: 6670
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: With this system it is possible to measure the activity of a ''virA'' receptor and the ''vir'' promoter, respectively. The reporter is a firefly luciferase from Promega's pGL4.10luc2 plasmid.
mutable notes: medium strong constitutive promotor, terminator to keep basal expression of luciferase low
mutable provenance: partsregistry, synthetic gene by Mr. Gene, pGL4.10luc2 by Promega, TI-plasmid from ''Agrobacterium tumefaciens'' C58
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K389003
component: BBa_B0010
component: BBa_K389004
component: BBa_K389002
component: BBa_J23110
sequence annotation: BBa_K389004
sequence annotation: BBa_K389002
sequence annotation: BBa_J23110
sequence annotation: BBa_K389003
sequence annotation: BBa_B0010
sequence: https://synbiohub.org/public/igem/BBa_K389012_sequence/1

BBa_K389003
type: Component
persistent identity: component2079841
display id: component2079841
version: 1
top level: BBa_K389012
access: public
definition: https://synbiohub.org/public/igem/BBa_K389003/1

BBa_B0010
type: Component
persistent identity: component2079835
display id: component2079835
version: 1
top level: BBa_K389012
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_K389004
type: Component
persistent identity: component2079844
display id: component2079844
version: 1
top level: BBa_K389012
access: public
definition: luc

BBa_K389002
type: Component
persistent identity: component2079832
display id: component2079832
version: 1
top level: BBa_K389012
access: public
definition: https://synbiohub.org/public/igem/BBa_K389002/1

BBa_B0010
type: Component
persistent identity: component2079833
display id: component2079833
version: 1
top level: BBa_K389012
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_J23110
type: Component
persistent identity: component2079828
display id: component2079828
version: 1
top level: BBa_K389012
access: public
definition: https://synbiohub.org/public/igem/BBa_J23110/1

BBa_K389004
type: SequenceAnnotation
persistent identity: annotation2079844
display id: annotation2079844
version: 1
top level: BBa_K389012
location: https://synbiohub.org/public/igem/BBa_K389012/annotation2079844/range2079844/1
component: BBa_K389004

https://synbiohub.org/public/igem/BBa_K389012/annotation2079844/range2079844/1
type: Range
persistent identity: range2079844
display id: range2079844
version: 1
direction: direction/reverse
top level: BBa_K389012
start: 1132
end: 2787
orientation: inline

BBa_K389002
type: SequenceAnnotation
persistent identity: annotation2079832
display id: annotation2079832
version: 1
top level: BBa_K389012
location: https://synbiohub.org/public/igem/BBa_K389012/annotation2079832/range2079832/1
component: BBa_K389002

https://synbiohub.org/public/igem/BBa_K389012/annotation2079832/range2079832/1
type: Range
persistent identity: range2079832
display id: range2079832
version: 1
direction: direction/reverse
top level: BBa_K389012
start: 44
end: 783
orientation: inline

BBa_J23110
type: SequenceAnnotation
persistent identity: annotation2079828
display id: annotation2079828
version: 1
top level: BBa_K389012
location: https://synbiohub.org/public/igem/BBa_K389012/annotation2079828/range2079828/1
component: BBa_J23110

https://synbiohub.org/public/igem/BBa_K389012/annotation2079828/range2079828/1
type: Range
persistent identity: range2079828
display id: range2079828
version: 1
direction: direction/reverse
top level: BBa_K389012
start: 1
end: 35
orientation: inline

BBa_K389003
type: SequenceAnnotation
persistent identity: annotation2079841
display id: annotation2079841
version: 1
top level: BBa_K389012
location: https://synbiohub.org/public/igem/BBa_K389012/annotation2079841/range2079841/1
component: BBa_K389003

https://synbiohub.org/public/igem/BBa_K389012/annotation2079841/range2079841/1
type: Range
persistent identity: range2079841
display id: range2079841
version: 1
direction: direction/reverse
top level: BBa_K389012
start: 968
end: 1125
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2079835
display id: annotation2079835
version: 1
top level: BBa_K389012
location: https://synbiohub.org/public/igem/BBa_K389012/annotation2079835/range2079835/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K389012/annotation2079835/range2079835/1
type: Range
persistent identity: range2079835
display id: range2079835
version: 1
direction: direction/forward
top level: BBa_K389012
start: 880
end: 959
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2079833
display id: annotation2079833
version: 1
top level: BBa_K389012
location: https://synbiohub.org/public/igem/BBa_K389012/annotation2079833/range2079833/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K389012/annotation2079833/range2079833/1
type: Range
persistent identity: range2079833
display id: range2079833
version: 1
direction: direction/forward
top level: BBa_K389012
start: 792
end: 871
orientation: inline

BBa_K222000
type: ComponentDefinition
persistent identity: BBa K222000
display id: BBa_K222000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Aequorin
creator: Cristina Vilanova
created: 2009-10-19T11:00:00Z
modified: 2015-05-08T01:11:31Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _327_
m user id: 0
owner id: 4909
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: The aequorin-coelenterazine complex needs calcium to produce light. In the process this calcium enters through a special type of calcium channels present in the cell???s plasma membrane which open or close in response to a change in the transmembrane potential, that is why they are called voltage-dependent calcium channels (VDCC). The equation which describes calcium current through these channels is the following: Where g is the conductance associated with the channel, V is the transmembrane potential and ECa is the Nernst potential, related to the different ionic concentration inside and outside the cell. Considering that these channels are only permeable to calcium and have two states -open or closed-, the total conductance associated with the population of VDCCs can be expressed as the maximal conductance ( ) times the fraction of all channels that are open. This fraction is determined by hypothetical activation and inactivation variables m and h, which depend on voltage and time: VDCCs are well characterized in excitable cells such as neurons, where the value of is known: Budding yeast, Saccharomyces cerevisiae has homologous voltage-dependent channels in its plasma membrane. However, there is a lack of study of the properties of these channels. That is why we have determined their conductance according to our experimental results: Having characterized calcium entry to the cell, we studied the aequorin production of light through a calcium-mediated response after two types of stimulation: ??? Chemical stimulation Alkali shock (performed by the addition of KOH) induced a calcium entry to cytoplasm (where aequorin is located) through voltage-dependent calcium channels. These calcium ions bind the aequorin-coelenterazine complex and photons ???light- are emitted during a short period of time. This graph shows yeasts??? response to the addition of 30 ??L of KOH: Furthermore, we made experiments with increasing KOH volumes (in microliters) and realized that light em…
mutable notes: -
mutable provenance: It comes from the genomic sequence of the luminiscent jellyfish Aequorea victoria.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence: https://synbiohub.org/public/igem/BBa_K222000_sequence/1

BBa_K1680025
type: ComponentDefinition
persistent identity: BBa K1680025
display id: BBa_K1680025
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RFP-luciferase Cre reporter
creator: Nicolai von Kuegelgen
created: 2015-09-16T11:00:00Z
modified: 2015-10-28T03:54:52Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2098_
m user id: 15693
owner id: 26366
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This part encodes a reporter cassette for the Cre Recombinase (e.g parts XYZ) in Yeast. An active Cre protein will switch expression from RFP to NanoLuc luciferase.
mutable notes: RFC10 assembly
mutable provenance: designed by Team Tuebingen
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_K1680005
component: BBa_K1680009
component: BBa_K392003
component: BBa_J63005
component: BBa_E1010
sequence annotation: BBa_J63005
sequence annotation: BBa_K1680005
sequence annotation: BBa_K1680009
sequence annotation: BBa_K392003
sequence annotation: BBa_E1010
sequence: https://synbiohub.org/public/igem/BBa_K1680025_sequence/1

BBa_K1680005
type: Component
persistent identity: component2466047
display id: component2466047
version: 1
top level: BBa_K1680025
access: public
definition: https://synbiohub.org/public/igem/BBa_K1680005/1

BBa_K1680009
type: Component
persistent identity: component2466053
display id: component2466053
version: 1
top level: BBa_K1680025
access: public
definition: BBa_K1680009

BBa_K1680005
type: Component
persistent identity: component2466052
display id: component2466052
version: 1
top level: BBa_K1680025
access: public
definition: https://synbiohub.org/public/igem/BBa_K1680005/1

BBa_K392003
type: Component
persistent identity: component2466051
display id: component2466051
version: 1
top level: BBa_K1680025
access: public
definition: https://synbiohub.org/public/igem/BBa_K392003/1

BBa_J63005
type: Component
persistent identity: component2466046
display id: component2466046
version: 1
top level: BBa_K1680025
access: public
definition: https://synbiohub.org/public/igem/BBa_J63005/1

BBa_E1010
type: Component
persistent identity: component2466050
display id: component2466050
version: 1
top level: BBa_K1680025
access: public
definition: https://synbiohub.org/public/igem/BBa_E1010/1

BBa_J63005
type: SequenceAnnotation
persistent identity: annotation2466046
display id: annotation2466046
version: 1
top level: BBa_K1680025
location: https://synbiohub.org/public/igem/BBa_K1680025/annotation2466046/range2466046/1
component: BBa_J63005

https://synbiohub.org/public/igem/BBa_K1680025/annotation2466046/range2466046/1
type: Range
persistent identity: range2466046
display id: range2466046
version: 1
direction: direction/reverse
top level: BBa_K1680025
start: 1
end: 1445
orientation: inline

BBa_K1680005
type: SequenceAnnotation
persistent identity: annotation2466047
display id: annotation2466047
version: 1
top level: BBa_K1680025
location: https://synbiohub.org/public/igem/BBa_K1680025/annotation2466047/range2466047/1
component: BBa_K1680005

https://synbiohub.org/public/igem/BBa_K1680025/annotation2466047/range2466047/1
type: Range
persistent identity: range2466047
display id: range2466047
version: 1
direction: direction/reverse
top level: BBa_K1680025
start: 1454
end: 1487
orientation: inline

BBa_K1680009
type: SequenceAnnotation
persistent identity: annotation2466053
display id: annotation2466053
version: 1
top level: BBa_K1680025
location: https://synbiohub.org/public/igem/BBa_K1680025/annotation2466053/range2466053/1
component: BBa_K1680009

https://synbiohub.org/public/igem/BBa_K1680025/annotation2466053/range2466053/1
type: Range
persistent identity: range2466053
display id: range2466053
version: 1
direction: direction/reverse
top level: BBa_K1680025
start: 2385
end: 2900
orientation: inline

BBa_K1680005
type: SequenceAnnotation
persistent identity: annotation2466052
display id: annotation2466052
version: 1
top level: BBa_K1680025
location: https://synbiohub.org/public/igem/BBa_K1680025/annotation2466052/range2466052/1
component: BBa_K1680005

https://synbiohub.org/public/igem/BBa_K1680025/annotation2466052/range2466052/1
type: Range
persistent identity: range2466052
display id: range2466052
version: 1
direction: direction/reverse
top level: BBa_K1680025
start: 2345
end: 2378
orientation: inline

BBa_K392003
type: SequenceAnnotation
persistent identity: annotation2466051
display id: annotation2466051
version: 1
top level: BBa_K1680025
location: https://synbiohub.org/public/igem/BBa_K1680025/annotation2466051/range2466051/1
component: BBa_K392003

https://synbiohub.org/public/igem/BBa_K1680025/annotation2466051/range2466051/1
type: Range
persistent identity: range2466051
display id: range2466051
version: 1
direction: direction/reverse
top level: BBa_K1680025
start: 2208
end: 2336
orientation: inline

BBa_E1010
type: SequenceAnnotation
persistent identity: annotation2466050
display id: annotation2466050
version: 1
top level: BBa_K1680025
location: https://synbiohub.org/public/igem/BBa_K1680025/annotation2466050/range2466050/1
component: BBa_E1010

https://synbiohub.org/public/igem/BBa_K1680025/annotation2466050/range2466050/1
type: Range
persistent identity: range2466050
display id: range2466050
version: 1
direction: direction/reverse
top level: BBa_K1680025
start: 1494
end: 2199
orientation: inline

BBa_K950004
type: ComponentDefinition
persistent identity: BBa K950004
display id: BBa_K950004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: firefly luciferase
creator: Jakob Matthes
created: 2012-07-28T11:00:00Z
modified: 2015-05-08T01:13:49Z
discontinued: false
dominant: false
group u list: _1218_
m user id: 0
owner id: 11576
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is a optimized firefly luciferase (C-terminal modification, see design notes).
mutable notes: Primers were designed to replace the C-terminal SKL aminoacids with KL.
mutable provenance: The original source is the Firefly luciferase, see http://www.ncbi.nlm.nih.gov/nuccore/AB644228.1 The modification is covered by 'One-step measurement of firefly luciferase activity in yeast' (http://www.ncbi.nlm.nih.gov/pubmed/14558144).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: luciferase
sequence annotation: XbaI
sequence: https://synbiohub.org/public/igem/BBa_K950004_sequence/1

luciferase
type: SequenceAnnotation
persistent identity: annotation2182744
display id: annotation2182744
version: 1
top level: BBa_K950004
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K950004/annotation2182744/range2182744/1

https://synbiohub.org/public/igem/BBa_K950004/annotation2182744/range2182744/1
type: Range
persistent identity: range2182744
display id: range2182744
version: 1
direction: direction/forward
top level: BBa_K950004
start: 1
end: 1650
orientation: inline

XbaI
type: SequenceAnnotation
persistent identity: annotation2182745
display id: annotation2182745
version: 1
top level: BBa_K950004
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K950004/annotation2182745/range2182745/1

https://synbiohub.org/public/igem/BBa_K950004/annotation2182745/range2182745/1
type: Range
persistent identity: range2182745
display id: range2182745
version: 1
direction: direction/forward
top level: BBa_K950004
start: 48
end: 54
orientation: inline

SP-AntiNIP
type: ComponentDefinition
persistent identity: BBa K157030
display id: BBa_K157030
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: SP-AntiNIP-GGGSlinker-Tm-Splitluciferase2/2
creator: iGEM Team Freiburg 2008
created: 2008-10-25T11:00:00Z
modified: 2015-05-08T01:10:55Z
discontinued: false
dominant: true
experience: experience/None
group u list: _232_
m user id: 0
owner id: 1673
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Part consists of the signal peptide of Erbb1/EGF-receptor, scFV-antiNIP, GGGSflexlinker (extracellular part), the trans-membrane region of the Erbb1/EGF-receptor and the C-terminal part of the split fluorophor YFP with a fluolinker joined to its N-Terminus (intracellular part).
mutable notes: none
mutable provenance: Derived by cloning of the parts BBa_K157001, BBa_K157003, BBa_K157013, BBa_K157002 and Part one of Split- Luciferase For further information see the description of the parts mentioned above.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: Scar (Thr, Gly)
sequence annotation: GGGS-Linker
sequence annotation: NgoMI
sequence annotation: SP
sequence annotation: Tm
sequence annotation: AgeI
sequence annotation: Splitluciferase2/2
sequence annotation: SP-AntiNIP-GGGSlinker-Tm-Splitluciferase2/2
sequence annotation: Sc-Fv_anti-NIP
sequence: https://synbiohub.org/public/igem/BBa_K157030_sequence/1

Scar (Thr, Gly)
type: SequenceAnnotation
persistent identity: annotation1993316
display id: annotation1993316
version: 1
top level: SP-AntiNIP
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157030/annotation1993316/range1993316/1

https://synbiohub.org/public/igem/BBa_K157030/annotation1993316/range1993316/1
type: Range
persistent identity: range1993316
display id: range1993316
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 949
end: 954
orientation: inline

GGGS-Linker
type: SequenceAnnotation
persistent identity: annotation1993313
display id: annotation1993313
version: 1
top level: SP-AntiNIP
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157030/annotation1993313/range1993313/1

https://synbiohub.org/public/igem/BBa_K157030/annotation1993313/range1993313/1
type: Range
persistent identity: range1993313
display id: range1993313
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 829
end: 873
orientation: inline

Scar (Thr, Gly)
type: SequenceAnnotation
persistent identity: annotation1993312
display id: annotation1993312
version: 1
top level: SP-AntiNIP
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157030/annotation1993312/range1993312/1

https://synbiohub.org/public/igem/BBa_K157030/annotation1993312/range1993312/1
type: Range
persistent identity: range1993312
display id: range1993312
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 823
end: 828
orientation: inline

NgoMI
type: SequenceAnnotation
persistent identity: annotation1990263
display id: annotation1990263
version: 1
top level: SP-AntiNIP
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157030/annotation1990263/range1990263/1

https://synbiohub.org/public/igem/BBa_K157030/annotation1990263/range1990263/1
type: Range
persistent identity: range1990263
display id: range1990263
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 4
end: 9
orientation: inline

Scar (Thr, Gly)
type: SequenceAnnotation
persistent identity: annotation1993310
display id: annotation1993310
version: 1
top level: SP-AntiNIP
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157030/annotation1993310/range1993310/1

https://synbiohub.org/public/igem/BBa_K157030/annotation1993310/range1993310/1
type: Range
persistent identity: range1993310
display id: range1993310
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 82
end: 87
orientation: inline

SP
type: SequenceAnnotation
persistent identity: annotation1993309
display id: annotation1993309
version: 1
top level: SP-AntiNIP
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157030/annotation1993309/range1993309/1

https://synbiohub.org/public/igem/BBa_K157030/annotation1993309/range1993309/1
type: Range
persistent identity: range1993309
display id: range1993309
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 10
end: 81
orientation: inline

Tm
type: SequenceAnnotation
persistent identity: annotation1993315
display id: annotation1993315
version: 1
top level: SP-AntiNIP
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157030/annotation1993315/range1993315/1

https://synbiohub.org/public/igem/BBa_K157030/annotation1993315/range1993315/1
type: Range
persistent identity: range1993315
display id: range1993315
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 880
end: 948
orientation: inline

AgeI
type: SequenceAnnotation
persistent identity: annotation1990264
display id: annotation1990264
version: 1
top level: SP-AntiNIP
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157030/annotation1990264/range1990264/1

https://synbiohub.org/public/igem/BBa_K157030/annotation1990264/range1990264/1
type: Range
persistent identity: range1990264
display id: range1990264
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 1231
end: 1236
orientation: inline

Splitluciferase2/2
type: SequenceAnnotation
persistent identity: annotation1993317
display id: annotation1993317
version: 1
top level: SP-AntiNIP
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157030/annotation1993317/range1993317/1

https://synbiohub.org/public/igem/BBa_K157030/annotation1993317/range1993317/1
type: Range
persistent identity: range1993317
display id: range1993317
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 955
end: 1230
orientation: inline

SP-AntiNIP-GGGSlinker-Tm-Splitluciferase2/2
type: SequenceAnnotation
persistent identity: annotation1990265
display id: annotation1990265
version: 1
top level: SP-AntiNIP
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K157030/annotation1990265/range1990265/1

https://synbiohub.org/public/igem/BBa_K157030/annotation1990265/range1990265/1
type: Range
persistent identity: range1990265
display id: range1990265
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 1
end: 1236
orientation: inline

Sc-Fv_anti-NIP
type: SequenceAnnotation
persistent identity: annotation1993311
display id: annotation1993311
version: 1
top level: SP-AntiNIP
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157030/annotation1993311/range1993311/1

https://synbiohub.org/public/igem/BBa_K157030/annotation1993311/range1993311/1
type: Range
persistent identity: range1993311
display id: range1993311
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 88
end: 822
orientation: inline

Scar (Thr, Gly)
type: SequenceAnnotation
persistent identity: annotation1993314
display id: annotation1993314
version: 1
top level: SP-AntiNIP
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157030/annotation1993314/range1993314/1

https://synbiohub.org/public/igem/BBa_K157030/annotation1993314/range1993314/1
type: Range
persistent identity: range1993314
display id: range1993314
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 874
end: 879
orientation: inline

SP-Lipocal
type: ComponentDefinition
persistent identity: BBa K157038
display id: BBa_K157038
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: SP-Lipocalin-GGGSlinker-Tm-Splitluciferase2/2
creator: iGEM Team Freiburg 2008
created: 2008-10-25T11:00:00Z
modified: 2015-05-08T01:10:55Z
discontinued: false
dominant: false
experience: experience/None
group u list: _232_
m user id: 0
owner id: 1673
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: A composite part consisting of the signal peptide of Erbb1/EGF-receptor, scFV-antiNIP, GGGSflexlinker (extracellular part), the trans-membrane region of the Erbb1/EGF-receptor and the C-terminal part of the split fluorophor YFP with a fluolinker joined to its N-Terminus (intracellular part).
mutable notes: none
mutable provenance: Derived by cloning of the parts BBa_K157001, BBa_K157004, BBa_K157013, BBa_K157002 and first half of split Luciferase For further information see the description of the parts mentioned above.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: Splitluciferase2/2
sequence annotation: AgeI
sequence annotation: Scar (Thr, Gly)
sequence annotation: NgoMI
sequence annotation: SP
sequence annotation: SP-Lipocalin-GGGSlinker-Tm-Splitluciferase2/2
sequence annotation: GGGS-Linker
sequence annotation: Tm
sequence annotation: Lipocalin
sequence: https://synbiohub.org/public/igem/BBa_K157038_sequence/1

Splitluciferase2/2
type: SequenceAnnotation
persistent identity: annotation1993426
display id: annotation1993426
version: 1
top level: SP-Lipocal
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157038/annotation1993426/range1993426/1

https://synbiohub.org/public/igem/BBa_K157038/annotation1993426/range1993426/1
type: Range
persistent identity: range1993426
display id: range1993426
version: 1
direction: direction/forward
top level: SP-Lipocal
start: 742
end: 1017
orientation: inline

AgeI
type: SequenceAnnotation
persistent identity: annotation1990301
display id: annotation1990301
version: 1
top level: SP-Lipocal
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157038/annotation1990301/range1990301/1

https://synbiohub.org/public/igem/BBa_K157038/annotation1990301/range1990301/1
type: Range
persistent identity: range1990301
display id: range1990301
version: 1
direction: direction/forward
top level: SP-Lipocal
start: 1018
end: 1023
orientation: inline

Scar (Thr, Gly)
type: SequenceAnnotation
persistent identity: annotation1993419
display id: annotation1993419
version: 1
top level: SP-Lipocal
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157038/annotation1993419/range1993419/1

https://synbiohub.org/public/igem/BBa_K157038/annotation1993419/range1993419/1
type: Range
persistent identity: range1993419
display id: range1993419
version: 1
direction: direction/forward
top level: SP-Lipocal
start: 82
end: 87
orientation: inline

NgoMI
type: SequenceAnnotation
persistent identity: annotation1990300
display id: annotation1990300
version: 1
top level: SP-Lipocal
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157038/annotation1990300/range1990300/1

https://synbiohub.org/public/igem/BBa_K157038/annotation1990300/range1990300/1
type: Range
persistent identity: range1990300
display id: range1990300
version: 1
direction: direction/forward
top level: SP-Lipocal
start: 4
end: 9
orientation: inline

SP
type: SequenceAnnotation
persistent identity: annotation1993418
display id: annotation1993418
version: 1
top level: SP-Lipocal
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157038/annotation1993418/range1993418/1

https://synbiohub.org/public/igem/BBa_K157038/annotation1993418/range1993418/1
type: Range
persistent identity: range1993418
display id: range1993418
version: 1
direction: direction/forward
top level: SP-Lipocal
start: 10
end: 81
orientation: inline

Scar (Thr, Gly)
type: SequenceAnnotation
persistent identity: annotation1993421
display id: annotation1993421
version: 1
top level: SP-Lipocal
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157038/annotation1993421/range1993421/1

https://synbiohub.org/public/igem/BBa_K157038/annotation1993421/range1993421/1
type: Range
persistent identity: range1993421
display id: range1993421
version: 1
direction: direction/forward
top level: SP-Lipocal
start: 610
end: 615
orientation: inline

SP-Lipocalin-GGGSlinker-Tm-Splitluciferase2/2
type: SequenceAnnotation
persistent identity: annotation1990302
display id: annotation1990302
version: 1
top level: SP-Lipocal
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K157038/annotation1990302/range1990302/1

https://synbiohub.org/public/igem/BBa_K157038/annotation1990302/range1990302/1
type: Range
persistent identity: range1990302
display id: range1990302
version: 1
direction: direction/forward
top level: SP-Lipocal
start: 1
end: 1023
orientation: inline

Scar (Thr, Gly)
type: SequenceAnnotation
persistent identity: annotation1993423
display id: annotation1993423
version: 1
top level: SP-Lipocal
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157038/annotation1993423/range1993423/1

https://synbiohub.org/public/igem/BBa_K157038/annotation1993423/range1993423/1
type: Range
persistent identity: range1993423
display id: range1993423
version: 1
direction: direction/forward
top level: SP-Lipocal
start: 661
end: 666
orientation: inline

Scar (Thr, Gly)
type: SequenceAnnotation
persistent identity: annotation1993425
display id: annotation1993425
version: 1
top level: SP-Lipocal
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157038/annotation1993425/range1993425/1

https://synbiohub.org/public/igem/BBa_K157038/annotation1993425/range1993425/1
type: Range
persistent identity: range1993425
display id: range1993425
version: 1
direction: direction/forward
top level: SP-Lipocal
start: 736
end: 741
orientation: inline

GGGS-Linker
type: SequenceAnnotation
persistent identity: annotation1993422
display id: annotation1993422
version: 1
top level: SP-Lipocal
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157038/annotation1993422/range1993422/1

https://synbiohub.org/public/igem/BBa_K157038/annotation1993422/range1993422/1
type: Range
persistent identity: range1993422
display id: range1993422
version: 1
direction: direction/forward
top level: SP-Lipocal
start: 616
end: 660
orientation: inline

Tm
type: SequenceAnnotation
persistent identity: annotation1993424
display id: annotation1993424
version: 1
top level: SP-Lipocal
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157038/annotation1993424/range1993424/1

https://synbiohub.org/public/igem/BBa_K157038/annotation1993424/range1993424/1
type: Range
persistent identity: range1993424
display id: range1993424
version: 1
direction: direction/forward
top level: SP-Lipocal
start: 667
end: 735
orientation: inline

Lipocalin
type: SequenceAnnotation
persistent identity: annotation1993420
display id: annotation1993420
version: 1
top level: SP-Lipocal
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157038/annotation1993420/range1993420/1

https://synbiohub.org/public/igem/BBa_K157038/annotation1993420/range1993420/1
type: Range
persistent identity: range1993420
display id: range1993420
version: 1
direction: direction/forward
top level: SP-Lipocal
start: 88
end: 609
orientation: inline

BBa_K389423
type: ComponentDefinition
persistent identity: BBa K389423
display id: BBa_K389423
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: VirA/G signaling system + sensitivity tuner + luciferase
creator: Jonas Aretz
created: 2010-10-10T11:00:00Z
modified: 2015-05-08T01:12:19Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _517_
m user id: 0
owner id: 6670
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part combines a complete VirA/G signaling system with a sensitivity tuner before a luciferase gene under the control of a ''vir'' promoter. The sensitivity tuner enhances a signal from a promoter so it enhances the readout luciferase.
mutable notes: sensitivity tuner to enhance the luciferase signal
mutable provenance: * luciferase from pGL4.10luc2 (Promega) * sensitivity tuner part, terminators and constitutive promoter from the partsregistry * ''virG'' was synthesized by Mr. Gene * ''virA'' and ''vir'' promoter from ''Agrobacterium tumefaciens'' C58, TI-plasmid
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K389017
component: BBa_K389403
sequence annotation: BBa_K389403
sequence annotation: BBa_K389017
sequence: https://synbiohub.org/public/igem/BBa_K389423_sequence/1

BBa_K389017
type: Component
persistent identity: component2313944
display id: component2313944
version: 1
top level: BBa_K389423
access: public
definition: https://synbiohub.org/public/igem/BBa_K389017/1

BBa_K389403
type: Component
persistent identity: component2313969
display id: component2313969
version: 1
top level: BBa_K389423
access: public
definition: BBa_K389403

BBa_K389403
type: SequenceAnnotation
persistent identity: annotation2313969
display id: annotation2313969
version: 1
top level: BBa_K389423
location: https://synbiohub.org/public/igem/BBa_K389423/annotation2313969/range2313969/1
component: BBa_K389403

https://synbiohub.org/public/igem/BBa_K389423/annotation2313969/range2313969/1
type: Range
persistent identity: range2313969
display id: range2313969
version: 1
direction: direction/reverse
top level: BBa_K389423
start: 3708
end: 6621
orientation: inline

BBa_K389017
type: SequenceAnnotation
persistent identity: annotation2313944
display id: annotation2313944
version: 1
top level: BBa_K389423
location: https://synbiohub.org/public/igem/BBa_K389423/annotation2313944/range2313944/1
component: BBa_K389017

https://synbiohub.org/public/igem/BBa_K389423/annotation2313944/range2313944/1
type: Range
persistent identity: range2313944
display id: range2313944
version: 1
direction: direction/reverse
top level: BBa_K389423
start: 1
end: 3699
orientation: inline

BBa_K1159001
type: ComponentDefinition
persistent identity: BBa K1159001
display id: BBa_K1159001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: NanoLuc Luciferase in RFC[25]
creator: TU Munich 2013
created: 2013-05-16T11:00:00Z
modified: 2016-01-26T02:12:53Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1471_
m user id: 4206
owner id: 14732
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: NanoLuc Luciferase
sequence: https://synbiohub.org/public/igem/BBa_K1159001_sequence/1

NanoLuc Luciferase
type: SequenceAnnotation
persistent identity: annotation2342942
display id: annotation2342942
version: 1
top level: BBa_K1159001
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1159001/annotation2342942/range2342942/1

https://synbiohub.org/public/igem/BBa_K1159001/annotation2342942/range2342942/1
type: Range
persistent identity: range2342942
display id: range2342942
version: 1
direction: direction/forward
top level: BBa_K1159001
start: 1
end: 510
orientation: inline

BBa_K389307
type: ComponentDefinition
persistent identity: BBa K389307
display id: BBa_K389307
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: medium strong constitutive promoter + luc
creator: Jonas Aretz
created: 2010-10-11T11:00:00Z
modified: 2015-05-08T01:12:19Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _517_
m user id: 0
owner id: 6670
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This is a firefly luciferase gene under the control of a medium strong constitutive promoter.
mutable notes: medium strong promoter, strong RBS, used this to demonstrate that our luciferase BioBrick is working
mutable provenance: * promoter and RBS from partsregistry * luciferase from pGL4.10luc2 (Promega)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0034
component: BBa_K389004
component: BBa_J23115
sequence annotation: BBa_B0034
sequence annotation: BBa_J23115
sequence annotation: BBa_K389004
sequence: https://synbiohub.org/public/igem/BBa_K389307_sequence/1

BBa_B0034
type: Component
persistent identity: component2085581
display id: component2085581
version: 1
top level: BBa_K389307
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K389004
type: Component
persistent identity: component2085584
display id: component2085584
version: 1
top level: BBa_K389307
access: public
definition: luc

BBa_J23115
type: Component
persistent identity: component2085579
display id: component2085579
version: 1
top level: BBa_K389307
access: public
definition: https://synbiohub.org/public/igem/BBa_J23115/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2085581
display id: annotation2085581
version: 1
top level: BBa_K389307
location: https://synbiohub.org/public/igem/BBa_K389307/annotation2085581/range2085581/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K389307/annotation2085581/range2085581/1
type: Range
persistent identity: range2085581
display id: range2085581
version: 1
direction: direction/reverse
top level: BBa_K389307
start: 44
end: 55
orientation: inline

BBa_J23115
type: SequenceAnnotation
persistent identity: annotation2085579
display id: annotation2085579
version: 1
top level: BBa_K389307
location: https://synbiohub.org/public/igem/BBa_K389307/annotation2085579/range2085579/1
component: BBa_J23115

https://synbiohub.org/public/igem/BBa_K389307/annotation2085579/range2085579/1
type: Range
persistent identity: range2085579
display id: range2085579
version: 1
direction: direction/reverse
top level: BBa_K389307
start: 1
end: 35
orientation: inline

BBa_K389004
type: SequenceAnnotation
persistent identity: annotation2085584
display id: annotation2085584
version: 1
top level: BBa_K389307
location: https://synbiohub.org/public/igem/BBa_K389307/annotation2085584/range2085584/1
component: BBa_K389004

https://synbiohub.org/public/igem/BBa_K389307/annotation2085584/range2085584/1
type: Range
persistent identity: range2085584
display id: range2085584
version: 1
direction: direction/reverse
top level: BBa_K389307
start: 62
end: 1717
orientation: inline

BBa_K1732029
type: ComponentDefinition
persistent identity: BBa K1732029
display id: BBa_K1732029
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: FireflyCO-His
creator: Donna Lee
created: 2015-09-17T11:00:00Z
modified: 2016-01-19T01:24:22Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2154_
m user id: 4206
owner id: 27195
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Firefly luciferase (BBa_I712019) with 6X-His tagged for easier protein purification. Sequence was codon optimized by IDT codon optimization tools.
mutable notes: 6X-His tagged for easier protein purification. Sequence was codon optimized by IDT codon optimization tools.
mutable provenance: BBa_I712019
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: FireflyCO
sequence annotation: 6XHis
sequence: https://synbiohub.org/public/igem/BBa_K1732029_sequence/1

FireflyCO
type: SequenceAnnotation
persistent identity: annotation2469654
display id: annotation2469654
version: 1
top level: BBa_K1732029
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1732029/annotation2469654/range2469654/1

https://synbiohub.org/public/igem/BBa_K1732029/annotation2469654/range2469654/1
type: Range
persistent identity: range2469654
display id: range2469654
version: 1
direction: direction/forward
top level: BBa_K1732029
start: 1
end: 1649
orientation: inline

6XHis
type: SequenceAnnotation
persistent identity: annotation2469655
display id: annotation2469655
version: 1
top level: BBa_K1732029
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1732029/annotation2469655/range2469655/1

https://synbiohub.org/public/igem/BBa_K1732029/annotation2469655/range2469655/1
type: Range
persistent identity: range2469655
display id: range2469655
version: 1
direction: direction/forward
top level: BBa_K1732029
start: 1650
end: 1668
orientation: inline

BBa_K216015
type: ComponentDefinition
persistent identity: BBa K216015
display id: BBa_K216015
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PyeaR with firefly luciferase
creator: Edinburgh iGEM 2009
created: 2009-10-15T11:00:00Z
modified: 2015-05-08T01:11:30Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _317_
m user id: 0
owner id: 837
owning group id: 163
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This is the nitrate- and nitrite-responsive promoter PyeaR of ''Escherichia coli'' with the luciferase of firefly ''Photinus pyralis'' (deposited by Slovenia iGEM 2007) with the addition of a strong bacterial ribosome binding site. It should generate light in the presence of nitrate/nitrite and luciferin.
mutable notes: No special considerations. Note that sequencing shows an unexpected insertion of 6 bases, ACCACC, after the scar between the ribosome binding site and the ATG of the luciferase coding sequence; that is, the sequence reads ACTAGACCACCATG rather than ACTAGATG. If this is correct, these 6 bases must be present in luciferase BioBrick BBa_I712019, or else were introduced by a random event during construction. This makes the RBS 12 bases from the ATG, somewhat more than the optimum, but with a strong RBS (like J15001) should still give about 25% of the expression level expected for optimum spacing in E. coli (Vellanoweth & Rabinowitz, 1992, Molecular Microbiology 6, 1105-1114).
mutable provenance: Made by joining PyeaR BioBrick BBa_K216005 to a PCR fusion product of strong synthetic ribosome binding site J15001 and firefly luciferase BioBrick BBa_I712019.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_K216005
component: BBa_J15001
component: BBa_I712019
sequence annotation: BBa_J15001
sequence annotation: BBa_I712019
sequence annotation: BBa_K216005
sequence: https://synbiohub.org/public/igem/BBa_K216015_sequence/1

BBa_K216005
type: Component
persistent identity: component2047099
display id: component2047099
version: 1
top level: BBa_K216015
access: public
definition: https://synbiohub.org/public/igem/BBa_K216005/1

BBa_J15001
type: Component
persistent identity: component2047102
display id: component2047102
version: 1
top level: BBa_K216015
access: public
definition: https://synbiohub.org/public/igem/BBa_J15001/1

BBa_I712019
type: Component
persistent identity: component2047103
display id: component2047103
version: 1
top level: BBa_K216015
access: public
definition: BBa_I712019

BBa_J15001
type: SequenceAnnotation
persistent identity: annotation2047102
display id: annotation2047102
version: 1
top level: BBa_K216015
location: https://synbiohub.org/public/igem/BBa_K216015/annotation2047102/range2047102/1
component: BBa_J15001

https://synbiohub.org/public/igem/BBa_K216015/annotation2047102/range2047102/1
type: Range
persistent identity: range2047102
display id: range2047102
version: 1
direction: direction/reverse
top level: BBa_K216015
start: 109
end: 118
orientation: inline

BBa_I712019
type: SequenceAnnotation
persistent identity: annotation2047103
display id: annotation2047103
version: 1
top level: BBa_K216015
location: https://synbiohub.org/public/igem/BBa_K216015/annotation2047103/range2047103/1
component: BBa_I712019

https://synbiohub.org/public/igem/BBa_K216015/annotation2047103/range2047103/1
type: Range
persistent identity: range2047103
display id: range2047103
version: 1
direction: direction/reverse
top level: BBa_K216015
start: 125
end: 1777
orientation: inline

BBa_K216005
type: SequenceAnnotation
persistent identity: annotation2047099
display id: annotation2047099
version: 1
top level: BBa_K216015
location: https://synbiohub.org/public/igem/BBa_K216015/annotation2047099/range2047099/1
component: BBa_K216005

https://synbiohub.org/public/igem/BBa_K216015/annotation2047099/range2047099/1
type: Range
persistent identity: range2047099
display id: range2047099
version: 1
direction: direction/reverse
top level: BBa_K216015
start: 1
end: 100
orientation: inline

BBa_K518002
type: ComponentDefinition
persistent identity: BBa K518002
display id: BBa_K518002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Firefly-renilla dual luciferase assay kit
creator: Masato Ohgishi
created: 2011-09-14T11:00:00Z
modified: 2015-05-08T01:12:33Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _683_
m user id: 0
owner id: 8305
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Luciferase assay is one of the most popular reporter assay system for quantitatively measuring the function of promoters and other cis-elements. The wide-ranging and quantitative detection is the prominent feature of this assay; light emmision from 10~(-18) ― 10~(-11) mol protein can be quantitatively measured using luminometer (see our results). A promoter or other cis-element to be analysed can be ligated above this part. Its effect will be measured as an altered firefly luciferase expression. Meanwhile, renilla luciferase expression can be used to as an internal control.
mutable notes: None
mutable provenance: From BioBrick parts.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_J23118
component: BBa_B0014
component: BBa_B0032
component: BBa_I712019
component: BBa_J52008
sequence annotation: BBa_B0032
sequence annotation: BBa_J23118
sequence annotation: BBa_I712019
sequence annotation: BBa_B0014
sequence annotation: BBa_J52008
sequence: https://synbiohub.org/public/igem/BBa_K518002_sequence/1

BBa_J23118
type: Component
persistent identity: component2128886
display id: component2128886
version: 1
top level: BBa_K518002
access: public
definition: https://synbiohub.org/public/igem/BBa_J23118/1

BBa_B0014
type: Component
persistent identity: component2128899
display id: component2128899
version: 1
top level: BBa_K518002
access: public
definition: https://synbiohub.org/public/igem/BBa_B0014/1

BBa_B0032
type: Component
persistent identity: component2128876
display id: component2128876
version: 1
top level: BBa_K518002
access: public
definition: https://synbiohub.org/public/igem/BBa_B0032/1

BBa_I712019
type: Component
persistent identity: component2128878
display id: component2128878
version: 1
top level: BBa_K518002
access: public
definition: BBa_I712019

BBa_B0032
type: Component
persistent identity: component2128888
display id: component2128888
version: 1
top level: BBa_K518002
access: public
definition: https://synbiohub.org/public/igem/BBa_B0032/1

BBa_J52008
type: Component
persistent identity: component2128892
display id: component2128892
version: 1
top level: BBa_K518002
access: public
definition: BBa_J52008

BBa_B0014
type: Component
persistent identity: component2128885
display id: component2128885
version: 1
top level: BBa_K518002
access: public
definition: https://synbiohub.org/public/igem/BBa_B0014/1

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation2128876
display id: annotation2128876
version: 1
top level: BBa_K518002
location: https://synbiohub.org/public/igem/BBa_K518002/annotation2128876/range2128876/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_K518002/annotation2128876/range2128876/1
type: Range
persistent identity: range2128876
display id: range2128876
version: 1
direction: direction/forward
top level: BBa_K518002
start: 1
end: 13
orientation: inline

BBa_J23118
type: SequenceAnnotation
persistent identity: annotation2128886
display id: annotation2128886
version: 1
top level: BBa_K518002
location: https://synbiohub.org/public/igem/BBa_K518002/annotation2128886/range2128886/1
component: BBa_J23118

https://synbiohub.org/public/igem/BBa_K518002/annotation2128886/range2128886/1
type: Range
persistent identity: range2128886
display id: range2128886
version: 1
direction: direction/reverse
top level: BBa_K518002
start: 1784
end: 1818
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation2128888
display id: annotation2128888
version: 1
top level: BBa_K518002
location: https://synbiohub.org/public/igem/BBa_K518002/annotation2128888/range2128888/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_K518002/annotation2128888/range2128888/1
type: Range
persistent identity: range2128888
display id: range2128888
version: 1
direction: direction/forward
top level: BBa_K518002
start: 1827
end: 1839
orientation: inline

BBa_I712019
type: SequenceAnnotation
persistent identity: annotation2128878
display id: annotation2128878
version: 1
top level: BBa_K518002
location: https://synbiohub.org/public/igem/BBa_K518002/annotation2128878/range2128878/1
component: BBa_I712019

https://synbiohub.org/public/igem/BBa_K518002/annotation2128878/range2128878/1
type: Range
persistent identity: range2128878
display id: range2128878
version: 1
direction: direction/reverse
top level: BBa_K518002
start: 20
end: 1672
orientation: inline

BBa_B0014
type: SequenceAnnotation
persistent identity: annotation2128885
display id: annotation2128885
version: 1
top level: BBa_K518002
location: https://synbiohub.org/public/igem/BBa_K518002/annotation2128885/range2128885/1
component: BBa_B0014

https://synbiohub.org/public/igem/BBa_K518002/annotation2128885/range2128885/1
type: Range
persistent identity: range2128885
display id: range2128885
version: 1
direction: direction/reverse
top level: BBa_K518002
start: 1681
end: 1775
orientation: inline

BBa_B0014
type: SequenceAnnotation
persistent identity: annotation2128899
display id: annotation2128899
version: 1
top level: BBa_K518002
location: https://synbiohub.org/public/igem/BBa_K518002/annotation2128899/range2128899/1
component: BBa_B0014

https://synbiohub.org/public/igem/BBa_K518002/annotation2128899/range2128899/1
type: Range
persistent identity: range2128899
display id: range2128899
version: 1
direction: direction/reverse
top level: BBa_K518002
start: 2790
end: 2884
orientation: inline

BBa_J52008
type: SequenceAnnotation
persistent identity: annotation2128892
display id: annotation2128892
version: 1
top level: BBa_K518002
location: https://synbiohub.org/public/igem/BBa_K518002/annotation2128892/range2128892/1
component: BBa_J52008

https://synbiohub.org/public/igem/BBa_K518002/annotation2128892/range2128892/1
type: Range
persistent identity: range2128892
display id: range2128892
version: 1
direction: direction/reverse
top level: BBa_K518002
start: 1846
end: 2781
orientation: inline

BBa_K1486055
type: ComponentDefinition
persistent identity: BBa K1486055
display id: BBa_K1486055
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CheY/CheZ + split fLuc
creator: EPFL iGem team 2014
created: 2014-10-06T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: to edit
mutable notes: to edit
mutable provenance: to edit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_K1486053
component: BBa_B0034
component: BBa_K569017
component: BBa_K1486016
component: BBa_K1486017
component: BBa_I0500
component: BBa_K629003
sequence annotation: BBa_K1486017
sequence annotation: BBa_K1486053
sequence annotation: BBa_K629003
sequence annotation: BBa_B0034
sequence annotation: BBa_K1486016
sequence annotation: BBa_I0500
sequence annotation: BBa_K569017
sequence: https://synbiohub.org/public/igem/BBa_K1486055_sequence/1

BBa_K1486053
type: Component
persistent identity: component2399894
display id: component2399894
version: 1
top level: BBa_K1486055
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486053/1

BBa_B0034
type: Component
persistent identity: component2399881
display id: component2399881
version: 1
top level: BBa_K1486055
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K569017
type: Component
persistent identity: component2399882
display id: component2399882
version: 1
top level: BBa_K1486055
access: public
definition: https://synbiohub.org/public/igem/BBa_K569017/1

BBa_B0034
type: Component
persistent identity: component2399886
display id: component2399886
version: 1
top level: BBa_K1486055
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K1486016
type: Component
persistent identity: component2399884
display id: component2399884
version: 1
top level: BBa_K1486055
access: public
definition: BBa_K1486016

BBa_K1486053
type: Component
persistent identity: component2399883
display id: component2399883
version: 1
top level: BBa_K1486055
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486053/1

BBa_K1486017
type: Component
persistent identity: component2399895
display id: component2399895
version: 1
top level: BBa_K1486055
access: public
definition: BBa_K1486017

BBa_I0500
type: Component
persistent identity: component2399879
display id: component2399879
version: 1
top level: BBa_K1486055
access: public
definition: https://synbiohub.org/public/igem/BBa_I0500/1

BBa_K629003
type: Component
persistent identity: component2399893
display id: component2399893
version: 1
top level: BBa_K1486055
access: public
definition: https://synbiohub.org/public/igem/BBa_K629003/1

BBa_K1486017
type: SequenceAnnotation
persistent identity: annotation2399895
display id: annotation2399895
version: 1
top level: BBa_K1486055
location: https://synbiohub.org/public/igem/BBa_K1486055/annotation2399895/range2399895/1
component: BBa_K1486017

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type: Range
persistent identity: range2399895
display id: range2399895
version: 1
direction: direction/reverse
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start: 2832
end: 4079
orientation: inline

BBa_K1486053
type: SequenceAnnotation
persistent identity: annotation2399894
display id: annotation2399894
version: 1
top level: BBa_K1486055
location: https://synbiohub.org/public/igem/BBa_K1486055/annotation2399894/range2399894/1
component: BBa_K1486053

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type: Range
persistent identity: range2399894
display id: range2399894
version: 1
direction: direction/reverse
top level: BBa_K1486055
start: 2802
end: 2831
orientation: inline

BBa_K629003
type: SequenceAnnotation
persistent identity: annotation2399893
display id: annotation2399893
version: 1
top level: BBa_K1486055
location: https://synbiohub.org/public/igem/BBa_K1486055/annotation2399893/range2399893/1
component: BBa_K629003

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type: Range
persistent identity: range2399893
display id: range2399893
version: 1
direction: direction/reverse
top level: BBa_K1486055
start: 2157
end: 2801
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2399881
display id: annotation2399881
version: 1
top level: BBa_K1486055
location: https://synbiohub.org/public/igem/BBa_K1486055/annotation2399881/range2399881/1
component: BBa_B0034

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type: Range
persistent identity: range2399881
display id: range2399881
version: 1
direction: direction/reverse
top level: BBa_K1486055
start: 1211
end: 1222
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2399886
display id: annotation2399886
version: 1
top level: BBa_K1486055
location: https://synbiohub.org/public/igem/BBa_K1486055/annotation2399886/range2399886/1
component: BBa_B0034

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type: Range
persistent identity: range2399886
display id: range2399886
version: 1
direction: direction/reverse
top level: BBa_K1486055
start: 2145
end: 2156
orientation: inline

BBa_K1486016
type: SequenceAnnotation
persistent identity: annotation2399884
display id: annotation2399884
version: 1
top level: BBa_K1486055
location: https://synbiohub.org/public/igem/BBa_K1486055/annotation2399884/range2399884/1
component: BBa_K1486016

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type: Range
persistent identity: range2399884
display id: range2399884
version: 1
direction: direction/reverse
top level: BBa_K1486055
start: 1671
end: 2144
orientation: inline

BBa_I0500
type: SequenceAnnotation
persistent identity: annotation2399879
display id: annotation2399879
version: 1
top level: BBa_K1486055
location: https://synbiohub.org/public/igem/BBa_K1486055/annotation2399879/range2399879/1
component: BBa_I0500

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type: Range
persistent identity: range2399879
display id: range2399879
version: 1
direction: direction/reverse
top level: BBa_K1486055
start: 1
end: 1210
orientation: inline

BBa_K1486053
type: SequenceAnnotation
persistent identity: annotation2399883
display id: annotation2399883
version: 1
top level: BBa_K1486055
location: https://synbiohub.org/public/igem/BBa_K1486055/annotation2399883/range2399883/1
component: BBa_K1486053

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type: Range
persistent identity: range2399883
display id: range2399883
version: 1
direction: direction/reverse
top level: BBa_K1486055
start: 1641
end: 1670
orientation: inline

BBa_K569017
type: SequenceAnnotation
persistent identity: annotation2399882
display id: annotation2399882
version: 1
top level: BBa_K1486055
location: https://synbiohub.org/public/igem/BBa_K1486055/annotation2399882/range2399882/1
component: BBa_K569017

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type: Range
persistent identity: range2399882
display id: range2399882
version: 1
direction: direction/reverse
top level: BBa_K1486055
start: 1223
end: 1640
orientation: inline

BBa_K1732027
type: ComponentDefinition
persistent identity: BBa K1732027
display id: BBa_K1732027
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: GLuc-His
creator: Donna Lee
created: 2015-09-17T11:00:00Z
modified: 2016-01-19T01:23:44Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2154_
m user id: 4206
owner id: 27195
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Gaussia Luciferase (BBa_K1732003) with 6X-His tag for easier protein purification. Sequence was codon optimized for E.coli via IDT codon optimization tools.
mutable notes: 6X-His tag for easier protein purification. Sequence was codon optimized for E.coli via IDT codon optimization tools.
mutable provenance: BBa_K1732003
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: 6X-His
sequence annotation: GLucCO
sequence: https://synbiohub.org/public/igem/BBa_K1732027_sequence/1

6X-His
type: SequenceAnnotation
persistent identity: annotation2469631
display id: annotation2469631
version: 1
top level: BBa_K1732027
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1732027/annotation2469631/range2469631/1

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type: Range
persistent identity: range2469631
display id: range2469631
version: 1
direction: direction/forward
top level: BBa_K1732027
start: 504
end: 522
orientation: inline

GLucCO
type: SequenceAnnotation
persistent identity: annotation2469630
display id: annotation2469630
version: 1
top level: BBa_K1732027
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1732027/annotation2469630/range2469630/1

https://synbiohub.org/public/igem/BBa_K1732027/annotation2469630/range2469630/1
type: Range
persistent identity: range2469630
display id: range2469630
version: 1
direction: direction/forward
top level: BBa_K1732027
start: 1
end: 503
orientation: inline

BBa_K284999
type: ComponentDefinition
persistent identity: BBa K284999
display id: BBa_K284999
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Deletar este tamb??m
creator: Johana Rincones
created: 2009-05-15T11:00:00Z
modified: 2015-05-08T01:11:48Z
discontinued: false
dominant: false
group u list: _386_
m user id: 0
owner id: 4137
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This version of the LuxC comes from Vibrio salmonicida. Team Example used it to build a bacterial lava lamp.
mutable notes: No modifications were necessary in order to comply with the standard.
mutable provenance: Vibrio salmonicida hypothetical protein EJN1 gene, complete cds; luminescence gene cluster, complete sequence; and RibG (ribG) gene, complete cds. NCBI Accession number is AF452135 REGION: 2346..3776
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Vs_LuxC protein coding sequence
sequence annotation: ATG start codon
sequence annotation: TAA Stop codon
sequence: https://synbiohub.org/public/igem/BBa_K284999_sequence/1

Vs_LuxC protein coding sequence
type: SequenceAnnotation
persistent identity: annotation2003117
display id: annotation2003117
version: 1
top level: BBa_K284999
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K284999/annotation2003117/range2003117/1

https://synbiohub.org/public/igem/BBa_K284999/annotation2003117/range2003117/1
type: Range
persistent identity: range2003117
display id: range2003117
version: 1
direction: direction/forward
top level: BBa_K284999
start: 1
end: 1431
orientation: inline

ATG start codon
type: SequenceAnnotation
persistent identity: annotation2003098
display id: annotation2003098
version: 1
top level: BBa_K284999
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K284999/annotation2003098/range2003098/1

https://synbiohub.org/public/igem/BBa_K284999/annotation2003098/range2003098/1
type: Range
persistent identity: range2003098
display id: range2003098
version: 1
direction: direction/forward
top level: BBa_K284999
start: 1
end: 3
orientation: inline

TAA Stop codon
type: SequenceAnnotation
persistent identity: annotation2003104
display id: annotation2003104
version: 1
top level: BBa_K284999
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K284999/annotation2003104/range2003104/1

https://synbiohub.org/public/igem/BBa_K284999/annotation2003104/range2003104/1
type: Range
persistent identity: range2003104
display id: range2003104
version: 1
direction: direction/forward
top level: BBa_K284999
start: 1429
end: 1431
orientation: inline

BBa_K1761002
type: ComponentDefinition
persistent identity: BBa K1761002
display id: BBa_K1761002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Outer Membrane Protein X (OmpX) with BsoBI-linker and NanoLuc
creator: Laura van Smeden
created: 2015-09-02T11:00:00Z
modified: 2015-09-18T05:40:03Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2183_
m user id: 25572
owner id: 25572
owning group id: 9
status: status/Planning
bookmark: false
mutable description: OmpX is an outer membrane protein with the C- and N-termini in the intracellulair domain. To be able to use OmpX as a scaffold, a non-natural amino acid needs to be introduced. This can be done by implementing the amber stop codon TAG in one of the loops of OmpX via a mutation. With a specific tRNA an azide-functionalized amino acid can be built in, which can be used for the SPAAC click chemistry reaction with DBCO functionalized groups. The BsoBI linker is a 213 bp long flexible GGSGGS linker. Using the restriction enzyme BsoBI, the linker can become 45 bp shorter. NanoLuc is a small sized and bright luciferase. Under the addition of furimazine, the NanoLuc luciferase generates a bioluminescece emmission spectrum with a maximum around 460 nm.
mutable notes: The sequence is redesigned considering codon optimalization. Also restriction sites for biobricking and classical cloning are deleted. From the BsoBI linker, the coding sequecne for GGSGGS is redesigned causing that only two BsoBI restriction sites remain.
mutable provenance: OmpX is a outer membrane protein naturally occuring in wildtype E.coli. The BsoBI linker is inspired by the article "Quantitative Understanding of the Energy Transfer between Fluorescent Proteins Connected via Flexible Peptide Liners" by Toon H. Evers et all from 29 August 2006. NanoLuc naturally occurs in the organism Oplophorus gracilirostris and was optimalized by the company Promega.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: BBa_K1761000
sequence annotation: BsoBI linker
sequence annotation: NanoLuc
sequence: https://synbiohub.org/public/igem/BBa_K1761002_sequence/1

BBa_K1761000
type: SequenceAnnotation
persistent identity: annotation2443222
display id: annotation2443222
version: 1
top level: BBa_K1761002
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1761002/annotation2443222/range2443222/1

https://synbiohub.org/public/igem/BBa_K1761002/annotation2443222/range2443222/1
type: Range
persistent identity: range2443222
display id: range2443222
version: 1
direction: direction/forward
top level: BBa_K1761002
start: 1
end: 513
orientation: inline

BsoBI linker
type: SequenceAnnotation
persistent identity: annotation2443223
display id: annotation2443223
version: 1
top level: BBa_K1761002
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1761002/annotation2443223/range2443223/1

https://synbiohub.org/public/igem/BBa_K1761002/annotation2443223/range2443223/1
type: Range
persistent identity: range2443223
display id: range2443223
version: 1
direction: direction/forward
top level: BBa_K1761002
start: 514
end: 726
orientation: inline

NanoLuc
type: SequenceAnnotation
persistent identity: annotation2443224
display id: annotation2443224
version: 1
top level: BBa_K1761002
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1761002/annotation2443224/range2443224/1

https://synbiohub.org/public/igem/BBa_K1761002/annotation2443224/range2443224/1
type: Range
persistent identity: range2443224
display id: range2443224
version: 1
direction: direction/forward
top level: BBa_K1761002
start: 727
end: 1239
orientation: inline

BBa_K1689009
type: ComponentDefinition
persistent identity: BBa K1689009
display id: BBa_K1689009
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: C-luc-dCas9
creator: ZHANG Yihao
created: 2015-09-15T11:00:00Z
modified: 2016-01-19T01:27:35Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2107_
m user id: 4206
owner id: 26434
owning group id: 9
status: status/Planning
bookmark: true
mutable description: coding sequence of the fusion protein of CFluc of the luciferase and the dCas
mutable notes: goldengate
mutable provenance: E.coli
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: CFluc398
sequence annotation: dCas9
sequence annotation: random spacer
sequence annotation: B0034
sequence annotation: stop codon
sequence annotation: GGGGSGGGGS linker
sequence annotation: rrnB T1 terminator
sequence annotation: start codon
sequence: https://synbiohub.org/public/igem/BBa_K1689009_sequence/1

CFluc398
type: SequenceAnnotation
persistent identity: annotation2473615
display id: annotation2473615
version: 1
top level: BBa_K1689009
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1689009/annotation2473615/range2473615/1

https://synbiohub.org/public/igem/BBa_K1689009/annotation2473615/range2473615/1
type: Range
persistent identity: range2473615
display id: range2473615
version: 1
direction: direction/forward
top level: BBa_K1689009
start: 81
end: 536
orientation: inline

dCas9
type: SequenceAnnotation
persistent identity: annotation2473617
display id: annotation2473617
version: 1
top level: BBa_K1689009
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1689009/annotation2473617/range2473617/1

https://synbiohub.org/public/igem/BBa_K1689009/annotation2473617/range2473617/1
type: Range
persistent identity: range2473617
display id: range2473617
version: 1
direction: direction/forward
top level: BBa_K1689009
start: 567
end: 4667
orientation: inline

random spacer
type: SequenceAnnotation
persistent identity: annotation2477741
display id: annotation2477741
version: 1
top level: BBa_K1689009
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1689009/annotation2477741/range2477741/1

https://synbiohub.org/public/igem/BBa_K1689009/annotation2477741/range2477741/1
type: Range
persistent identity: range2477741
display id: range2477741
version: 1
direction: direction/forward
top level: BBa_K1689009
start: 1
end: 59
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation2473613
display id: annotation2473613
version: 1
top level: BBa_K1689009
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1689009/annotation2473613/range2473613/1

https://synbiohub.org/public/igem/BBa_K1689009/annotation2473613/range2473613/1
type: Range
persistent identity: range2473613
display id: range2473613
version: 1
direction: direction/forward
top level: BBa_K1689009
start: 60
end: 77
orientation: inline

stop codon
type: SequenceAnnotation
persistent identity: annotation2473618
display id: annotation2473618
version: 1
top level: BBa_K1689009
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1689009/annotation2473618/range2473618/1

https://synbiohub.org/public/igem/BBa_K1689009/annotation2473618/range2473618/1
type: Range
persistent identity: range2473618
display id: range2473618
version: 1
direction: direction/forward
top level: BBa_K1689009
start: 4668
end: 4670
orientation: inline

GGGGSGGGGS linker
type: SequenceAnnotation
persistent identity: annotation2473616
display id: annotation2473616
version: 1
top level: BBa_K1689009
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1689009/annotation2473616/range2473616/1

https://synbiohub.org/public/igem/BBa_K1689009/annotation2473616/range2473616/1
type: Range
persistent identity: range2473616
display id: range2473616
version: 1
direction: direction/forward
top level: BBa_K1689009
start: 537
end: 566
orientation: inline

rrnB T1 terminator
type: SequenceAnnotation
persistent identity: annotation2473619
display id: annotation2473619
version: 1
top level: BBa_K1689009
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1689009/annotation2473619/range2473619/1

https://synbiohub.org/public/igem/BBa_K1689009/annotation2473619/range2473619/1
type: Range
persistent identity: range2473619
display id: range2473619
version: 1
direction: direction/forward
top level: BBa_K1689009
start: 4686
end: 4764
orientation: inline

random spacer
type: SequenceAnnotation
persistent identity: annotation2477742
display id: annotation2477742
version: 1
top level: BBa_K1689009
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1689009/annotation2477742/range2477742/1

https://synbiohub.org/public/igem/BBa_K1689009/annotation2477742/range2477742/1
type: Range
persistent identity: range2477742
display id: range2477742
version: 1
direction: direction/forward
top level: BBa_K1689009
start: 4765
end: 4841
orientation: inline

start codon
type: SequenceAnnotation
persistent identity: annotation2473614
display id: annotation2473614
version: 1
top level: BBa_K1689009
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1689009/annotation2473614/range2473614/1

https://synbiohub.org/public/igem/BBa_K1689009/annotation2473614/range2473614/1
type: Range
persistent identity: range2473614
display id: range2473614
version: 1
direction: direction/forward
top level: BBa_K1689009
start: 78
end: 80
orientation: inline

BBa_K518013
type: ComponentDefinition
persistent identity: BBa K518013
display id: BBa_K518013
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: sulA promoter evaluation device
creator: Masato Ohgishi
created: 2011-10-02T11:00:00Z
modified: 2015-05-08T01:12:33Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _683_
m user id: 0
owner id: 8305
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: Microbes including Escherichia coli are known to respond to various DNA-injuring stress (ionizing radiation, ultraviolet radiation, peroxides etc...), altering their gene expressions. This response, known as "SOS response", are induced by regulatory protein called RecA, which is activated when binds to single-strand DNA. DNA-RecA complex promotes the self-degradation of LexA, a common repressor for SOS genes. SulA is responsible for stress-induced halt of cell division. The promoter of sulA, or sulAp, is acutely induced by various stress factors, including ultraviolet irradiation. We provide a sulAp evaluation device to make it easy to measure the varying expression of sulAp in various "stressful" conditions. Employing a dual luciferase reporter construct, quantitative measurement and comparison of Relative Promoter Unit (RPU) is achieved.
mutable notes: sulAp + dual luciferase reporter construct
mutable provenance: sulAp (BBa_K518010) was cloned from Escherichia coli K12 strain's genome.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_K518010
component: BBa_K518002
sequence annotation: BBa_K518010
sequence annotation: BBa_K518002
sequence: https://synbiohub.org/public/igem/BBa_K518013_sequence/1

BBa_K518010
type: Component
persistent identity: component2151395
display id: component2151395
version: 1
top level: BBa_K518013
access: public
definition: https://synbiohub.org/public/igem/BBa_K518010/1

BBa_K518002
type: Component
persistent identity: component2151421
display id: component2151421
version: 1
top level: BBa_K518013
access: public
definition: BBa_K518002

BBa_K518010
type: SequenceAnnotation
persistent identity: annotation2151395
display id: annotation2151395
version: 1
top level: BBa_K518013
location: https://synbiohub.org/public/igem/BBa_K518013/annotation2151395/range2151395/1
component: BBa_K518010

https://synbiohub.org/public/igem/BBa_K518013/annotation2151395/range2151395/1
type: Range
persistent identity: range2151395
display id: range2151395
version: 1
direction: direction/reverse
top level: BBa_K518013
start: 1
end: 52
orientation: inline

BBa_K518002
type: SequenceAnnotation
persistent identity: annotation2151421
display id: annotation2151421
version: 1
top level: BBa_K518013
location: https://synbiohub.org/public/igem/BBa_K518013/annotation2151421/range2151421/1
component: BBa_K518002

https://synbiohub.org/public/igem/BBa_K518013/annotation2151421/range2151421/1
type: Range
persistent identity: range2151421
display id: range2151421
version: 1
direction: direction/reverse
top level: BBa_K518013
start: 61
end: 2944
orientation: inline

BBa_J52013
type: ComponentDefinition
persistent identity: BBa J52013
display id: BBa_J52013
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: dnMyD88-linker-Rluc-linker-PEST191
creator: Monika Ciglic
created: 2006-10-13T11:00:00Z
modified: 2015-08-31T03:54:04Z
discontinued: false
dominant: false
group u list: _80_
m user id: 0
owner id: 800
owning group id: 80
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: dnMyD88-linker-Rluc-linker-PEST191 is a part where dnMyD88 (very important protein in signaling cascade through TLR4 receptor) is linked to Rluc (reporter) and PEST191 (degradation tag). Both linkers are made of six aminoacids.
mutable notes: dnMyD88-linker-Rluc-linker-PEST191 was made by using PCR ligation technique. It was difficult since we had some problems with primers. This part is cloned in BioBrick vector pSB1AK3.
mutable provenance: -
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: linker
sequence annotation: dnMyD88
sequence annotation: PEST191
sequence annotation: dnMyD88-linker-Rluc-linker-PEST191
sequence annotation: Rluc
sequence: https://synbiohub.org/public/igem/BBa_J52013_sequence/1

linker
type: SequenceAnnotation
persistent identity: annotation1902843
display id: annotation1902843
version: 1
top level: BBa_J52013
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_J52013/annotation1902843/range1902843/1

https://synbiohub.org/public/igem/BBa_J52013/annotation1902843/range1902843/1
type: Range
persistent identity: range1902843
display id: range1902843
version: 1
direction: direction/forward
top level: BBa_J52013
start: 1369
end: 1386
orientation: inline

dnMyD88
type: SequenceAnnotation
persistent identity: annotation1902840
display id: annotation1902840
version: 1
top level: BBa_J52013
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J52013/annotation1902840/range1902840/1

https://synbiohub.org/public/igem/BBa_J52013/annotation1902840/range1902840/1
type: Range
persistent identity: range1902840
display id: range1902840
version: 1
direction: direction/forward
top level: BBa_J52013
start: 1
end: 417
orientation: inline

PEST191
type: SequenceAnnotation
persistent identity: annotation1902844
display id: annotation1902844
version: 1
top level: BBa_J52013
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_J52013/annotation1902844/range1902844/1

https://synbiohub.org/public/igem/BBa_J52013/annotation1902844/range1902844/1
type: Range
persistent identity: range1902844
display id: range1902844
version: 1
direction: direction/forward
top level: BBa_J52013
start: 1387
end: 1872
orientation: inline

dnMyD88-linker-Rluc-linker-PEST191
type: SequenceAnnotation
persistent identity: annotation1902848
display id: annotation1902848
version: 1
top level: BBa_J52013
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_J52013/annotation1902848/range1902848/1

https://synbiohub.org/public/igem/BBa_J52013/annotation1902848/range1902848/1
type: Range
persistent identity: range1902848
display id: range1902848
version: 1
direction: direction/forward
top level: BBa_J52013
start: 1
end: 1872
orientation: inline

linker
type: SequenceAnnotation
persistent identity: annotation1902841
display id: annotation1902841
version: 1
top level: BBa_J52013
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_J52013/annotation1902841/range1902841/1

https://synbiohub.org/public/igem/BBa_J52013/annotation1902841/range1902841/1
type: Range
persistent identity: range1902841
display id: range1902841
version: 1
direction: direction/forward
top level: BBa_J52013
start: 418
end: 435
orientation: inline

Rluc
type: SequenceAnnotation
persistent identity: annotation1902842
display id: annotation1902842
version: 1
top level: BBa_J52013
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J52013/annotation1902842/range1902842/1

https://synbiohub.org/public/igem/BBa_J52013/annotation1902842/range1902842/1
type: Range
persistent identity: range1902842
display id: range1902842
version: 1
direction: direction/forward
top level: BBa_J52013
start: 436
end: 1368
orientation: inline

BBa_K785003
type: ComponentDefinition
persistent identity: BBa K785003
display id: BBa_K785003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Lux(CDABEG) coding gene PoPS->Light
creator: Zining Hou
created: 2012-09-22T11:00:00Z
modified: 2015-05-08T01:13:22Z
discontinued: false
dominant: false
group u list: _1040_
m user id: 0
owner id: 11000
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This page described the Lux operon from Vibrio fischeri. Lux gene is directly PCRed from the BBa_K325909, the pbad operon is deleted and now this part is available for any expression unit. You can provide light out put by putting this coding device under your promoter. We have successfully put this coding sequence into the PET28a expression system (cell strain is dH5α), and light out put could induced by the IPTG. To see more information about Lux light generator, please visit http://partsregistry.org/Part:BBa_K325909, and a more complete description can be found on the http://2010.igem.org/Team:Cambridge/Bioluminescence/G28.
mutable notes: In our project, we have to put the Lux light generator gene under the Ptet operon. So we have to relieve this part from the Pbad operon.
mutable provenance: We use PCR extract the DNA sequence from BBa_K325909, and clone it into the PSB1C3.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: putative rbs region
sequence annotation: luxD
sequence annotation: luxA
sequence annotation: luxB
sequence annotation: luxC
sequence annotation: luxE
sequence annotation: luxG
sequence: https://synbiohub.org/public/igem/BBa_K785003_sequence/1

putative rbs region
type: SequenceAnnotation
persistent identity: annotation2193990
display id: annotation2193990
version: 1
top level: BBa_K785003
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K785003/annotation2193990/range2193990/1

https://synbiohub.org/public/igem/BBa_K785003/annotation2193990/range2193990/1
type: Range
persistent identity: range2193990
display id: range2193990
version: 1
direction: direction/forward
top level: BBa_K785003
start: 2406
end: 2411
orientation: inline

luxD
type: SequenceAnnotation
persistent identity: annotation2193987
display id: annotation2193987
version: 1
top level: BBa_K785003
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K785003/annotation2193987/range2193987/1

https://synbiohub.org/public/igem/BBa_K785003/annotation2193987/range2193987/1
type: Range
persistent identity: range2193987
display id: range2193987
version: 1
direction: direction/forward
top level: BBa_K785003
start: 1465
end: 2368
orientation: inline

luxA
type: SequenceAnnotation
persistent identity: annotation2193993
display id: annotation2193993
version: 1
top level: BBa_K785003
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K785003/annotation2193993/range2193993/1

https://synbiohub.org/public/igem/BBa_K785003/annotation2193993/range2193993/1
type: Range
persistent identity: range2193993
display id: range2193993
version: 1
direction: direction/forward
top level: BBa_K785003
start: 2419
end: 3483
orientation: inline

luxB
type: SequenceAnnotation
persistent identity: annotation2193996
display id: annotation2193996
version: 1
top level: BBa_K785003
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K785003/annotation2193996/range2193996/1

https://synbiohub.org/public/igem/BBa_K785003/annotation2193996/range2193996/1
type: Range
persistent identity: range2193996
display id: range2193996
version: 1
direction: direction/forward
top level: BBa_K785003
start: 3522
end: 4502
orientation: inline

putative rbs region
type: SequenceAnnotation
persistent identity: annotation2193994
display id: annotation2193994
version: 1
top level: BBa_K785003
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K785003/annotation2193994/range2193994/1

https://synbiohub.org/public/igem/BBa_K785003/annotation2193994/range2193994/1
type: Range
persistent identity: range2193994
display id: range2193994
version: 1
direction: direction/forward
top level: BBa_K785003
start: 3509
end: 3514
orientation: inline

putative rbs region
type: SequenceAnnotation
persistent identity: annotation2193989
display id: annotation2193989
version: 1
top level: BBa_K785003
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K785003/annotation2193989/range2193989/1

https://synbiohub.org/public/igem/BBa_K785003/annotation2193989/range2193989/1
type: Range
persistent identity: range2193989
display id: range2193989
version: 1
direction: direction/forward
top level: BBa_K785003
start: 1454
end: 1458
orientation: inline

luxC
type: SequenceAnnotation
persistent identity: annotation2193988
display id: annotation2193988
version: 1
top level: BBa_K785003
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K785003/annotation2193988/range2193988/1

https://synbiohub.org/public/igem/BBa_K785003/annotation2193988/range2193988/1
type: Range
persistent identity: range2193988
display id: range2193988
version: 1
direction: direction/forward
top level: BBa_K785003
start: 21
end: 1450
orientation: inline

putative rbs region
type: SequenceAnnotation
persistent identity: annotation2193986
display id: annotation2193986
version: 1
top level: BBa_K785003
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K785003/annotation2193986/range2193986/1

https://synbiohub.org/public/igem/BBa_K785003/annotation2193986/range2193986/1
type: Range
persistent identity: range2193986
display id: range2193986
version: 1
direction: direction/forward
top level: BBa_K785003
start: 1
end: 20
orientation: inline

putative rbs region
type: SequenceAnnotation
persistent identity: annotation2193999
display id: annotation2193999
version: 1
top level: BBa_K785003
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K785003/annotation2193999/range2193999/1

https://synbiohub.org/public/igem/BBa_K785003/annotation2193999/range2193999/1
type: Range
persistent identity: range2193999
display id: range2193999
version: 1
direction: direction/forward
top level: BBa_K785003
start: 5705
end: 5709
orientation: inline

luxE
type: SequenceAnnotation
persistent identity: annotation2193998
display id: annotation2193998
version: 1
top level: BBa_K785003
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K785003/annotation2193998/range2193998/1

https://synbiohub.org/public/igem/BBa_K785003/annotation2193998/range2193998/1
type: Range
persistent identity: range2193998
display id: range2193998
version: 1
direction: direction/forward
top level: BBa_K785003
start: 4616
end: 5713
orientation: inline

luxG
type: SequenceAnnotation
persistent identity: annotation2194000
display id: annotation2194000
version: 1
top level: BBa_K785003
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K785003/annotation2194000/range2194000/1

https://synbiohub.org/public/igem/BBa_K785003/annotation2194000/range2194000/1
type: Range
persistent identity: range2194000
display id: range2194000
version: 1
direction: direction/forward
top level: BBa_K785003
start: 5717
end: 6427
orientation: inline

putative rbs region
type: SequenceAnnotation
persistent identity: annotation2193997
display id: annotation2193997
version: 1
top level: BBa_K785003
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K785003/annotation2193997/range2193997/1

https://synbiohub.org/public/igem/BBa_K785003/annotation2193997/range2193997/1
type: Range
persistent identity: range2193997
display id: range2193997
version: 1
direction: direction/forward
top level: BBa_K785003
start: 4565
end: 4571
orientation: inline

BBa_K819008
type: ComponentDefinition
persistent identity: BBa K819008
display id: BBa_K819008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Lux Operon under T7 promoter
creator: ZHANG Zidong
created: 2012-09-02T11:00:00Z
modified: 2015-05-08T01:13:29Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1077_
m user id: 0
owner id: 13785
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: luxABCDE gene ligated to the rear of T7 promoter. When transformed into BL21(DE3) E.coli strain, add different amount IPTG to reach different final concentration of IPTG in the medium can induce different level of Lux ligt emission. No substrate is needed.
mutable notes: luxABCDE gene ligated to the rear of T7 promoter. The original Ara and P-bad were replaced with T7 promoter, which enables its usage in our project.
mutable provenance: T7 promoter and LuxCDABE with their RBS are from Partsregistry.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: BBa_K819008
sequence annotation: LuxCDABEG with corresponding six RBS(from K325909)
sequence: https://synbiohub.org/public/igem/BBa_K819008_sequence/1

BBa_K819008
type: SequenceAnnotation
persistent identity: annotation2197308
display id: annotation2197308
version: 1
top level: BBa_K819008
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K819008/annotation2197308/range2197308/1

https://synbiohub.org/public/igem/BBa_K819008/annotation2197308/range2197308/1
type: Range
persistent identity: range2197308
display id: range2197308
version: 1
direction: direction/forward
top level: BBa_K819008
start: 1
end: 46
orientation: inline

LuxCDABEG with corresponding six RBS(from K325909)
type: SequenceAnnotation
persistent identity: annotation2197309
display id: annotation2197309
version: 1
top level: BBa_K819008
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K819008/annotation2197309/range2197309/1

https://synbiohub.org/public/igem/BBa_K819008/annotation2197309/range2197309/1
type: Range
persistent identity: range2197309
display id: range2197309
version: 1
direction: direction/forward
top level: BBa_K819008
start: 53
end: 6480
orientation: inline

BBa_K1486033
type: ComponentDefinition
persistent identity: BBa K1486033
display id: BBa_K1486033
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: R.reniformis luciferase + ADH1 terminator + CaUra3
creator: EPFL iGem team 2014
created: 2014-09-26T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: To edit
mutable notes: To edit
mutable provenance: To edit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000324
role: partType/Tag
component: BBa_K1486031
component: BBa_K1486020
component: BBa_K1486037
component: BBa_K1486019
component: BBa_K1486025
sequence annotation: BBa_K1486031
sequence annotation: BBa_K1486037
sequence annotation: BBa_K1486019
sequence annotation: BBa_K1486025
sequence annotation: BBa_K1486020
sequence: https://synbiohub.org/public/igem/BBa_K1486033_sequence/1

BBa_K1486031
type: Component
persistent identity: component2418790
display id: component2418790
version: 1
top level: BBa_K1486033
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486031/1

BBa_K1486020
type: Component
persistent identity: component2418784
display id: component2418784
version: 1
top level: BBa_K1486033
access: public
definition: BBa_K1486020

BBa_K1486037
type: Component
persistent identity: component2418782
display id: component2418782
version: 1
top level: BBa_K1486033
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486037/1

BBa_K1486019
type: Component
persistent identity: component2418783
display id: component2418783
version: 1
top level: BBa_K1486033
access: public
definition: BBa_K1486019

BBa_K1486025
type: Component
persistent identity: component2418786
display id: component2418786
version: 1
top level: BBa_K1486033
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486025/1

BBa_K1486031
type: SequenceAnnotation
persistent identity: annotation2418790
display id: annotation2418790
version: 1
top level: BBa_K1486033
location: https://synbiohub.org/public/igem/BBa_K1486033/annotation2418790/range2418790/1
component: BBa_K1486031

https://synbiohub.org/public/igem/BBa_K1486033/annotation2418790/range2418790/1
type: Range
persistent identity: range2418790
display id: range2418790
version: 1
direction: direction/reverse
top level: BBa_K1486033
start: 1164
end: 2523
orientation: inline

BBa_K1486037
type: SequenceAnnotation
persistent identity: annotation2418782
display id: annotation2418782
version: 1
top level: BBa_K1486033
location: https://synbiohub.org/public/igem/BBa_K1486033/annotation2418782/range2418782/1
component: BBa_K1486037

https://synbiohub.org/public/igem/BBa_K1486033/annotation2418782/range2418782/1
type: Range
persistent identity: range2418782
display id: range2418782
version: 1
direction: direction/reverse
top level: BBa_K1486033
start: 1
end: 39
orientation: inline

BBa_K1486019
type: SequenceAnnotation
persistent identity: annotation2418783
display id: annotation2418783
version: 1
top level: BBa_K1486033
location: https://synbiohub.org/public/igem/BBa_K1486033/annotation2418783/range2418783/1
component: BBa_K1486019

https://synbiohub.org/public/igem/BBa_K1486033/annotation2418783/range2418783/1
type: Range
persistent identity: range2418783
display id: range2418783
version: 1
direction: direction/reverse
top level: BBa_K1486033
start: 40
end: 369
orientation: inline

BBa_K1486025
type: SequenceAnnotation
persistent identity: annotation2418786
display id: annotation2418786
version: 1
top level: BBa_K1486033
location: https://synbiohub.org/public/igem/BBa_K1486033/annotation2418786/range2418786/1
component: BBa_K1486025

https://synbiohub.org/public/igem/BBa_K1486033/annotation2418786/range2418786/1
type: Range
persistent identity: range2418786
display id: range2418786
version: 1
direction: direction/reverse
top level: BBa_K1486033
start: 976
end: 1163
orientation: inline

BBa_K1486020
type: SequenceAnnotation
persistent identity: annotation2418784
display id: annotation2418784
version: 1
top level: BBa_K1486033
location: https://synbiohub.org/public/igem/BBa_K1486033/annotation2418784/range2418784/1
component: BBa_K1486020

https://synbiohub.org/public/igem/BBa_K1486033/annotation2418784/range2418784/1
type: Range
persistent identity: range2418784
display id: range2418784
version: 1
direction: direction/reverse
top level: BBa_K1486033
start: 370
end: 975
orientation: inline

BBa_K322237
type: ComponentDefinition
persistent identity: BBa K322237
display id: BBa_K322237
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Firefly luciferase from Photinus pyralis
creator: Chris French and William Galdric Rostain
created: 2010-09-25T11:00:00Z
modified: 2016-01-28T12:23:44Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _441_
m user id: 4206
owner id: 6225
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: In luminescent reactions, light is produced by the oxidation of a luciferin (a pigment): luciferin + O2 → oxyluciferin + light Thus luciferase is a a reporter protein when connected to other proteins or promoters prodicing light, by the oxidation of substrate luciferin. This is derived from BioBrick I712019, but PCR was used to remove the extra bases before the start codon (these are shown in the Registry sequencing data but are not indicated in the sequence as entered by Ljubljana 2007; presumably they are there to make a proper eukaryotic context for the start codon). This BioBrick contains the bare coding sequence in pSB1C3.
mutable notes: Forward sequence looks good. Reverse sequence missing the base between the stop codon and SpeI site. This is also missing in the S284T clone (see below) suggesting that it is missing in the primer, or did we use a vector based primer? In which case it would suggest that it was missing in the original clone. Trivial but annoying.
mutable provenance: The origional sequence is from Photinus pyralis, a the gene here is derived from BioBrick BBa-I712019.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K322237_sequence/1

BB123
type: ComponentDefinition
persistent identity: BBa K2023001
display id: BBa_K2023001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Biosensor device for BTEX detection
creator: Celia Chenebault, Camille Soucies, Benjamin Piot
created: 2016-10-13T11:00:00Z
modified: 2016-10-20T12:05:53Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2490_
m user id: 30230
owner id: 30230
owning group id: 9
status: status/Planning
bookmark: false
mutable description: The Pr promoter is a constitutive promoter driving the transcription of the XylR gene, coding for the XylR protein. The XylR protein, a transcriptional regulatory protein for the Pu promoter, is activated by aromatic hydrocarbons that carry a methyl group (like toluene and xylene). In our biosensor the Pu promoter allows the transcription of the bioluminescent reporter gene GLuc, coding for the Gaussia luciferase. When this enzyme reacts with its coelenterazine substrate it emits luminescence.
mutable notes: Site directed mutagenesis to overcome the mutation in the start codon of XylR
mutable provenance: IDT synthesis in 3 parts that were then assembled
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_K2023003
component: BBa_K2023002
sequence annotation: BBa_K2023003
sequence annotation: BBa_K2023002
sequence: https://synbiohub.org/public/igem/BBa_K2023001_sequence/1

BBa_K2023003
type: Component
persistent identity: component2518727
display id: component2518727
version: 1
top level: BB123
access: public
definition: BBa_K2023003

BBa_K2023002
type: Component
persistent identity: component2518713
display id: component2518713
version: 1
top level: BB123
access: public
definition: https://synbiohub.org/public/igem/BBa_K2023002/1

BBa_K2023003
type: SequenceAnnotation
persistent identity: annotation2518727
display id: annotation2518727
version: 1
top level: BB123
location: https://synbiohub.org/public/igem/BBa_K2023001/annotation2518727/range2518727/1
component: BBa_K2023003

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type: Range
persistent identity: range2518727
display id: range2518727
version: 1
direction: direction/reverse
top level: BB123
start: 2252
end: 3261
orientation: inline

BBa_K2023002
type: SequenceAnnotation
persistent identity: annotation2518713
display id: annotation2518713
version: 1
top level: BB123
location: https://synbiohub.org/public/igem/BBa_K2023001/annotation2518713/range2518713/1
component: BBa_K2023002

https://synbiohub.org/public/igem/BBa_K2023001/annotation2518713/range2518713/1
type: Range
persistent identity: range2518713
display id: range2518713
version: 1
direction: direction/reverse
top level: BB123
start: 1
end: 2243
orientation: inline

BBa_K389411
type: ComponentDefinition
persistent identity: BBa K389411
display id: BBa_K389411
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: vir promoter + sensitivity tuner + luciferase
creator: Jonas Aretz
created: 2010-10-10T11:00:00Z
modified: 2015-05-08T01:12:19Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _517_
m user id: 0
owner id: 6670
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part combines a mutated ''virG'' response regulator from the VirA/G receptor system under the control of a constitutive promoter with a sensitivity tuner before a luciferase gene under the control of a ''vir'' promoter. The sensitivity tuner enhances a signal from a promoter so it enhances the readout luciferase.
mutable notes: sensitivity tuner to enhance the luciferase signal
mutable provenance: * luciferase from pGL4.10luc2 (Promega) * sensitivity tuner part, terminators and constitutive promoter from the partsregistry * ''virG'' was synthesized by Mr. Gene * ''vir'' promoter from ''Agrobacterium tumefaciens'' C58, TI-plasmid
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K389401
component: BBa_J23110
component: BBa_B0017
component: BBa_K389003
component: BBa_K389002
sequence annotation: BBa_K389002
sequence annotation: BBa_K389003
sequence annotation: BBa_K389401
sequence annotation: BBa_B0017
sequence annotation: BBa_J23110
sequence: https://synbiohub.org/public/igem/BBa_K389411_sequence/1

BBa_K389401
type: Component
persistent identity: component2300760
display id: component2300760
version: 1
top level: BBa_K389411
access: public
definition: BBa_K389401

BBa_J23110
type: Component
persistent identity: component2300722
display id: component2300722
version: 1
top level: BBa_K389411
access: public
definition: https://synbiohub.org/public/igem/BBa_J23110/1

BBa_B0017
type: Component
persistent identity: component2300731
display id: component2300731
version: 1
top level: BBa_K389411
access: public
definition: https://synbiohub.org/public/igem/BBa_B0017/1

BBa_K389003
type: Component
persistent identity: component2300736
display id: component2300736
version: 1
top level: BBa_K389411
access: public
definition: https://synbiohub.org/public/igem/BBa_K389003/1

BBa_K389002
type: Component
persistent identity: component2300726
display id: component2300726
version: 1
top level: BBa_K389411
access: public
definition: https://synbiohub.org/public/igem/BBa_K389002/1

BBa_K389002
type: SequenceAnnotation
persistent identity: annotation2300726
display id: annotation2300726
version: 1
top level: BBa_K389411
location: https://synbiohub.org/public/igem/BBa_K389411/annotation2300726/range2300726/1
component: BBa_K389002

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type: Range
persistent identity: range2300726
display id: range2300726
version: 1
direction: direction/reverse
top level: BBa_K389411
start: 44
end: 783
orientation: inline

BBa_K389003
type: SequenceAnnotation
persistent identity: annotation2300736
display id: annotation2300736
version: 1
top level: BBa_K389411
location: https://synbiohub.org/public/igem/BBa_K389411/annotation2300736/range2300736/1
component: BBa_K389003

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type: Range
persistent identity: range2300736
display id: range2300736
version: 1
direction: direction/reverse
top level: BBa_K389411
start: 968
end: 1125
orientation: inline

BBa_K389401
type: SequenceAnnotation
persistent identity: annotation2300760
display id: annotation2300760
version: 1
top level: BBa_K389411
location: https://synbiohub.org/public/igem/BBa_K389411/annotation2300760/range2300760/1
component: BBa_K389401

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type: Range
persistent identity: range2300760
display id: range2300760
version: 1
direction: direction/reverse
top level: BBa_K389411
start: 1134
end: 4020
orientation: inline

BBa_B0017
type: SequenceAnnotation
persistent identity: annotation2300731
display id: annotation2300731
version: 1
top level: BBa_K389411
location: https://synbiohub.org/public/igem/BBa_K389411/annotation2300731/range2300731/1
component: BBa_B0017

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type: Range
persistent identity: range2300731
display id: range2300731
version: 1
direction: direction/reverse
top level: BBa_K389411
start: 792
end: 959
orientation: inline

BBa_J23110
type: SequenceAnnotation
persistent identity: annotation2300722
display id: annotation2300722
version: 1
top level: BBa_K389411
location: https://synbiohub.org/public/igem/BBa_K389411/annotation2300722/range2300722/1
component: BBa_J23110

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type: Range
persistent identity: range2300722
display id: range2300722
version: 1
direction: direction/reverse
top level: BBa_K389411
start: 1
end: 35
orientation: inline

BBa_K2023006
type: ComponentDefinition
persistent identity: BBa K2023006
display id: BBa_K2023006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Term-Pu-RBS-Gluc-term
creator: C??lia Chenebault
created: 2016-10-13T11:00:00Z
modified: 2016-10-20T01:08:42Z
discontinued: false
dominant: false
group u list: _2490_
m user id: 30356
owner id: 30230
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Term-Pu-RBS-Gluc-term (BB3)
mutable notes: blabla
mutable provenance: IDT synthesis
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_I723020
component: BBa_B0034
component: BBa_K2023009
component: BBa_B0014
sequence annotation: BBa_B0014
sequence annotation: BBa_B0034
sequence annotation: BBa_I723020
sequence annotation: BBa_K2023009
sequence: https://synbiohub.org/public/igem/BBa_K2023006_sequence/1

BBa_I723020
type: Component
persistent identity: component2518736
display id: component2518736
version: 1
top level: BBa_K2023006
access: public
definition: https://synbiohub.org/public/igem/BBa_I723020/1

BBa_B0034
type: Component
persistent identity: component2518738
display id: component2518738
version: 1
top level: BBa_K2023006
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K2023009
type: Component
persistent identity: component2518740
display id: component2518740
version: 1
top level: BBa_K2023006
access: public
definition: BBa_K2023009

BBa_B0014
type: Component
persistent identity: component2518734
display id: component2518734
version: 1
top level: BBa_K2023006
access: public
definition: https://synbiohub.org/public/igem/BBa_B0014/1

BBa_B0014
type: Component
persistent identity: component2518747
display id: component2518747
version: 1
top level: BBa_K2023006
access: public
definition: https://synbiohub.org/public/igem/BBa_B0014/1

BBa_B0014
type: SequenceAnnotation
persistent identity: annotation2518747
display id: annotation2518747
version: 1
top level: BBa_K2023006
location: https://synbiohub.org/public/igem/BBa_K2023006/annotation2518747/range2518747/1
component: BBa_B0014

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type: Range
persistent identity: range2518747
display id: range2518747
version: 1
direction: direction/reverse
top level: BBa_K2023006
start: 1019
end: 1113
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2518738
display id: annotation2518738
version: 1
top level: BBa_K2023006
location: https://synbiohub.org/public/igem/BBa_K2023006/annotation2518738/range2518738/1
component: BBa_B0034

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type: Range
persistent identity: range2518738
display id: range2518738
version: 1
direction: direction/reverse
top level: BBa_K2023006
start: 432
end: 443
orientation: inline

BBa_I723020
type: SequenceAnnotation
persistent identity: annotation2518736
display id: annotation2518736
version: 1
top level: BBa_K2023006
location: https://synbiohub.org/public/igem/BBa_K2023006/annotation2518736/range2518736/1
component: BBa_I723020

https://synbiohub.org/public/igem/BBa_K2023006/annotation2518736/range2518736/1
type: Range
persistent identity: range2518736
display id: range2518736
version: 1
direction: direction/reverse
top level: BBa_K2023006
start: 104
end: 423
orientation: inline

BBa_B0014
type: SequenceAnnotation
persistent identity: annotation2518734
display id: annotation2518734
version: 1
top level: BBa_K2023006
location: https://synbiohub.org/public/igem/BBa_K2023006/annotation2518734/range2518734/1
component: BBa_B0014

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type: Range
persistent identity: range2518734
display id: range2518734
version: 1
direction: direction/reverse
top level: BBa_K2023006
start: 1
end: 95
orientation: inline

BBa_K2023009
type: SequenceAnnotation
persistent identity: annotation2518740
display id: annotation2518740
version: 1
top level: BBa_K2023006
location: https://synbiohub.org/public/igem/BBa_K2023006/annotation2518740/range2518740/1
component: BBa_K2023009

https://synbiohub.org/public/igem/BBa_K2023006/annotation2518740/range2518740/1
type: Range
persistent identity: range2518740
display id: range2518740
version: 1
direction: direction/reverse
top level: BBa_K2023006
start: 450
end: 1010
orientation: inline

BBa_K1486016
type: ComponentDefinition
persistent identity: BBa K1486016
display id: BBa_K1486016
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: fLuc[1]
creator: EPFL iGem team 2014
created: 2014-09-14T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is the first part of the split Firefly luciferase. To be used with fLuc[2].
mutable notes: to edit
mutable provenance: to edit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence: https://synbiohub.org/public/igem/BBa_K1486016_sequence/1

BBa_K389403
type: ComponentDefinition
persistent identity: BBa K389403
display id: BBa_K389403
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Sensitivity tuner + luciferase
creator: Jonas Aretz
created: 2010-10-10T11:00:00Z
modified: 2015-05-08T01:12:19Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _517_
m user id: 0
owner id: 6670
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This is a modified <partinfo>BBa_I746390</partinfo> sensitivity tuner. Instead of a GFP reporter, this one has a firefly luciferase (from Promega's pGL4.10luc2 vector) as one reporter gene. The arabinose promoter has been removed from the original part so now it is possible to assemble this part behind any promoter to get an enhanced luciferase signal.
mutable notes: arabinose promoter removed, GFP exchanged with luciferase compared to the original part <partinfo>BBa_I746370</partinfo>
mutable provenance: * luciferase from pGL4.10luc2 (Promega) * the rest from the partsregistry
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_B0034
component: BBa_E1010
component: BBa_I746352
component: BBa_B0015
component: BBa_K389004
component: BBa_I746360
sequence annotation: BBa_B0034
sequence annotation: BBa_I746360
sequence annotation: BBa_B0015
sequence annotation: BBa_K389004
sequence annotation: BBa_I746352
sequence annotation: BBa_E1010
sequence: https://synbiohub.org/public/igem/BBa_K389403_sequence/1

BBa_B0034
type: Component
persistent identity: component2273103
display id: component2273103
version: 1
top level: BBa_K389403
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_E1010
type: Component
persistent identity: component2273106
display id: component2273106
version: 1
top level: BBa_K389403
access: public
definition: https://synbiohub.org/public/igem/BBa_E1010/1

BBa_B0034
type: Component
persistent identity: component2273117
display id: component2273117
version: 1
top level: BBa_K389403
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_I746352
type: Component
persistent identity: component2273101
display id: component2273101
version: 1
top level: BBa_K389403
access: public
definition: https://synbiohub.org/public/igem/BBa_I746352/1

BBa_B0015
type: Component
persistent identity: component2273113
display id: component2273113
version: 1
top level: BBa_K389403
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_K389004
type: Component
persistent identity: component2273120
display id: component2273120
version: 1
top level: BBa_K389403
access: public
definition: luc

BBa_I746360
type: Component
persistent identity: component2273115
display id: component2273115
version: 1
top level: BBa_K389403
access: public
definition: https://synbiohub.org/public/igem/BBa_I746360/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2273117
display id: annotation2273117
version: 1
top level: BBa_K389403
location: https://synbiohub.org/public/igem/BBa_K389403/annotation2273117/range2273117/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K389403/annotation2273117/range2273117/1
type: Range
persistent identity: range2273117
display id: range2273117
version: 1
direction: direction/reverse
top level: BBa_K389403
start: 1241
end: 1252
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2273103
display id: annotation2273103
version: 1
top level: BBa_K389403
location: https://synbiohub.org/public/igem/BBa_K389403/annotation2273103/range2273103/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K389403/annotation2273103/range2273103/1
type: Range
persistent identity: range2273103
display id: range2273103
version: 1
direction: direction/reverse
top level: BBa_K389403
start: 273
end: 284
orientation: inline

BBa_I746360
type: SequenceAnnotation
persistent identity: annotation2273115
display id: annotation2273115
version: 1
top level: BBa_K389403
location: https://synbiohub.org/public/igem/BBa_K389403/annotation2273115/range2273115/1
component: BBa_I746360

https://synbiohub.org/public/igem/BBa_K389403/annotation2273115/range2273115/1
type: Range
persistent identity: range2273115
display id: range2273115
version: 1
direction: direction/reverse
top level: BBa_K389403
start: 1142
end: 1232
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2273113
display id: annotation2273113
version: 1
top level: BBa_K389403
location: https://synbiohub.org/public/igem/BBa_K389403/annotation2273113/range2273113/1
component: BBa_B0015

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type: Range
persistent identity: range2273113
display id: range2273113
version: 1
direction: direction/reverse
top level: BBa_K389403
start: 1005
end: 1133
orientation: inline

BBa_K389004
type: SequenceAnnotation
persistent identity: annotation2273120
display id: annotation2273120
version: 1
top level: BBa_K389403
location: https://synbiohub.org/public/igem/BBa_K389403/annotation2273120/range2273120/1
component: BBa_K389004

https://synbiohub.org/public/igem/BBa_K389403/annotation2273120/range2273120/1
type: Range
persistent identity: range2273120
display id: range2273120
version: 1
direction: direction/reverse
top level: BBa_K389403
start: 1259
end: 2914
orientation: inline

BBa_I746352
type: SequenceAnnotation
persistent identity: annotation2273101
display id: annotation2273101
version: 1
top level: BBa_K389403
location: https://synbiohub.org/public/igem/BBa_K389403/annotation2273101/range2273101/1
component: BBa_I746352

https://synbiohub.org/public/igem/BBa_K389403/annotation2273101/range2273101/1
type: Range
persistent identity: range2273101
display id: range2273101
version: 1
direction: direction/reverse
top level: BBa_K389403
start: 1
end: 264
orientation: inline

BBa_E1010
type: SequenceAnnotation
persistent identity: annotation2273106
display id: annotation2273106
version: 1
top level: BBa_K389403
location: https://synbiohub.org/public/igem/BBa_K389403/annotation2273106/range2273106/1
component: BBa_E1010

https://synbiohub.org/public/igem/BBa_K389403/annotation2273106/range2273106/1
type: Range
persistent identity: range2273106
display id: range2273106
version: 1
direction: direction/reverse
top level: BBa_K389403
start: 291
end: 996
orientation: inline

BBa_K1734004
type: ComponentDefinition
persistent identity: BBa K1734004
display id: BBa_K1734004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Nanoluc (Codon optimized for Sf9 cells)
creator: Eduardo Ram??rez Montiel
created: 2015-09-17T11:00:00Z
modified: 2016-01-21T02:26:16Z
discontinued: false
dominant: false
group u list: _2156_
m user id: 4206
owner id: 20170
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Nluc luciferase is an enzyme that was engineered to work as a luminescent reporter weighing 19.1 kDa. This protein uses fumarizine as novel substrate to produce a high intensity glow-type luminescence. These reactions are ATP-independent, trying to obtain the maximal assay sensitivity. Reference: [1] Promega (2015). Nanoluc Genetic Reporter Vectors. URL: http://worldwide.promega.com/products/reporter-assays-and-transfection/reporter-vectors-and-cell-lines/nanoluc-luciferase-reporters/nanoluc-genetic-reporter-vectors/
mutable notes: For our project purposes its use is destined to work as a reporter of protein expression
mutable provenance: Promega's product
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Nanoluc
sequence: https://synbiohub.org/public/igem/BBa_K1734004_sequence/1

Nanoluc
type: SequenceAnnotation
persistent identity: annotation2470074
display id: annotation2470074
version: 1
top level: BBa_K1734004
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1734004/annotation2470074/range2470074/1

https://synbiohub.org/public/igem/BBa_K1734004/annotation2470074/range2470074/1
type: Range
persistent identity: range2470074
display id: range2470074
version: 1
direction: direction/forward
top level: BBa_K1734004
start: 1
end: 525
orientation: inline

BBa_K2023008
type: ComponentDefinition
persistent identity: BBa K2023008
display id: BBa_K2023008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Gluc-term
creator: C??lia Chenebault
created: 2016-10-13T11:00:00Z
modified: 2016-10-20T04:15:21Z
discontinued: false
dominant: false
group u list: _2490_
m user id: 30356
owner id: 30230
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Gluc-term
mutable notes: blabla
mutable provenance: IDT Synthesis
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0014
component: BBa_K2023009
sequence annotation: BBa_K2023009
sequence annotation: BBa_B0014
sequence: https://synbiohub.org/public/igem/BBa_K2023008_sequence/1

BBa_B0014
type: Component
persistent identity: component2519756
display id: component2519756
version: 1
top level: BBa_K2023008
access: public
definition: https://synbiohub.org/public/igem/BBa_B0014/1

BBa_K2023009
type: Component
persistent identity: component2519749
display id: component2519749
version: 1
top level: BBa_K2023008
access: public
definition: BBa_K2023009

BBa_K2023009
type: SequenceAnnotation
persistent identity: annotation2519749
display id: annotation2519749
version: 1
top level: BBa_K2023008
location: https://synbiohub.org/public/igem/BBa_K2023008/annotation2519749/range2519749/1
component: BBa_K2023009

https://synbiohub.org/public/igem/BBa_K2023008/annotation2519749/range2519749/1
type: Range
persistent identity: range2519749
display id: range2519749
version: 1
direction: direction/reverse
top level: BBa_K2023008
start: 1
end: 561
orientation: inline

BBa_B0014
type: SequenceAnnotation
persistent identity: annotation2519756
display id: annotation2519756
version: 1
top level: BBa_K2023008
location: https://synbiohub.org/public/igem/BBa_K2023008/annotation2519756/range2519756/1
component: BBa_B0014

https://synbiohub.org/public/igem/BBa_K2023008/annotation2519756/range2519756/1
type: Range
persistent identity: range2519756
display id: range2519756
version: 1
direction: direction/reverse
top level: BBa_K2023008
start: 570
end: 664
orientation: inline

BBa_K389051
type: ComponentDefinition
persistent identity: BBa K389051
display id: BBa_K389051
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Tightly regulated lacO + luc
creator: Jonas Aretz
created: 2010-10-07T11:00:00Z
modified: 2015-05-08T01:12:19Z
discontinued: false
dominant: false
experience: experience/Fails
group u list: _517_
m user id: 0
owner id: 6670
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is the tightly regulated lac operon <partinfo>K389050</partinfo> with a luciferase gene as readout.
mutable notes: lacIq integrated in promoter so good regulation, luciferase as sensitive readout
mutable provenance: partsregistry, pGL4.10luc2
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0034
component: BBa_K389004
component: BBa_K389050
sequence annotation: BBa_B0034
sequence annotation: BBa_K389050
sequence annotation: BBa_K389004
sequence: https://synbiohub.org/public/igem/BBa_K389051_sequence/1

BBa_B0034
type: Component
persistent identity: component2273070
display id: component2273070
version: 1
top level: BBa_K389051
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K389004
type: Component
persistent identity: component2273073
display id: component2273073
version: 1
top level: BBa_K389051
access: public
definition: luc

BBa_K389050
type: Component
persistent identity: component2273068
display id: component2273068
version: 1
top level: BBa_K389051
access: public
definition: https://synbiohub.org/public/igem/BBa_K389050/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2273070
display id: annotation2273070
version: 1
top level: BBa_K389051
location: https://synbiohub.org/public/igem/BBa_K389051/annotation2273070/range2273070/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K389051/annotation2273070/range2273070/1
type: Range
persistent identity: range2273070
display id: range2273070
version: 1
direction: direction/reverse
top level: BBa_K389051
start: 1609
end: 1620
orientation: inline

BBa_K389050
type: SequenceAnnotation
persistent identity: annotation2273068
display id: annotation2273068
version: 1
top level: BBa_K389051
location: https://synbiohub.org/public/igem/BBa_K389051/annotation2273068/range2273068/1
component: BBa_K389050

https://synbiohub.org/public/igem/BBa_K389051/annotation2273068/range2273068/1
type: Range
persistent identity: range2273068
display id: range2273068
version: 1
direction: direction/reverse
top level: BBa_K389051
start: 1
end: 1600
orientation: inline

BBa_K389004
type: SequenceAnnotation
persistent identity: annotation2273073
display id: annotation2273073
version: 1
top level: BBa_K389051
location: https://synbiohub.org/public/igem/BBa_K389051/annotation2273073/range2273073/1
component: BBa_K389004

https://synbiohub.org/public/igem/BBa_K389051/annotation2273073/range2273073/1
type: Range
persistent identity: range2273073
display id: range2273073
version: 1
direction: direction/reverse
top level: BBa_K389051
start: 1627
end: 3282
orientation: inline

BBa_K389402
type: ComponentDefinition
persistent identity: BBa K389402
display id: BBa_K389402
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Sensitivity tuner + luciferase
creator: Jonas Aretz
created: 2010-10-10T11:00:00Z
modified: 2015-05-08T01:12:19Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _517_
m user id: 0
owner id: 6670
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This is a modified <partinfo>BBa_I746380</partinfo> sensitivity tuner. Instead of a GFP reporter, this one has a firefly luciferase (from Promega's pGL4.10luc2 vector) as one reporter gene. The arabinose promoter has been removed from the original part so now it is possible to assemble this part behind any promoter to get an enhanced luciferase signal.
mutable notes: arabinose promoter removed, GFP exchanged with luciferase compared to the original part <partinfo>BBa_I746370</partinfo>
mutable provenance: * luciferase from pGL4.10luc2 (Promega) * the rest from the partsregistry
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_I746351
component: BBa_E1010
component: BBa_K389004
component: BBa_B0034
component: BBa_B0015
component: BBa_I746360
sequence annotation: BBa_K389004
sequence annotation: BBa_I746351
sequence annotation: BBa_B0015
sequence annotation: BBa_B0034
sequence annotation: BBa_I746360
sequence annotation: BBa_E1010
sequence: https://synbiohub.org/public/igem/BBa_K389402_sequence/1

BBa_I746351
type: Component
persistent identity: component2273077
display id: component2273077
version: 1
top level: BBa_K389402
access: public
definition: https://synbiohub.org/public/igem/BBa_I746351/1

BBa_E1010
type: Component
persistent identity: component2273082
display id: component2273082
version: 1
top level: BBa_K389402
access: public
definition: https://synbiohub.org/public/igem/BBa_E1010/1

BBa_K389004
type: Component
persistent identity: component2273096
display id: component2273096
version: 1
top level: BBa_K389402
access: public
definition: luc

BBa_B0034
type: Component
persistent identity: component2273079
display id: component2273079
version: 1
top level: BBa_K389402
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0015
type: Component
persistent identity: component2273089
display id: component2273089
version: 1
top level: BBa_K389402
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_B0034
type: Component
persistent identity: component2273093
display id: component2273093
version: 1
top level: BBa_K389402
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_I746360
type: Component
persistent identity: component2273091
display id: component2273091
version: 1
top level: BBa_K389402
access: public
definition: https://synbiohub.org/public/igem/BBa_I746360/1

BBa_K389004
type: SequenceAnnotation
persistent identity: annotation2273096
display id: annotation2273096
version: 1
top level: BBa_K389402
location: https://synbiohub.org/public/igem/BBa_K389402/annotation2273096/range2273096/1
component: BBa_K389004

https://synbiohub.org/public/igem/BBa_K389402/annotation2273096/range2273096/1
type: Range
persistent identity: range2273096
display id: range2273096
version: 1
direction: direction/reverse
top level: BBa_K389402
start: 1232
end: 2887
orientation: inline

BBa_I746351
type: SequenceAnnotation
persistent identity: annotation2273077
display id: annotation2273077
version: 1
top level: BBa_K389402
location: https://synbiohub.org/public/igem/BBa_K389402/annotation2273077/range2273077/1
component: BBa_I746351

https://synbiohub.org/public/igem/BBa_K389402/annotation2273077/range2273077/1
type: Range
persistent identity: range2273077
display id: range2273077
version: 1
direction: direction/reverse
top level: BBa_K389402
start: 1
end: 237
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2273089
display id: annotation2273089
version: 1
top level: BBa_K389402
location: https://synbiohub.org/public/igem/BBa_K389402/annotation2273089/range2273089/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K389402/annotation2273089/range2273089/1
type: Range
persistent identity: range2273089
display id: range2273089
version: 1
direction: direction/reverse
top level: BBa_K389402
start: 978
end: 1106
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2273079
display id: annotation2273079
version: 1
top level: BBa_K389402
location: https://synbiohub.org/public/igem/BBa_K389402/annotation2273079/range2273079/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K389402/annotation2273079/range2273079/1
type: Range
persistent identity: range2273079
display id: range2273079
version: 1
direction: direction/reverse
top level: BBa_K389402
start: 246
end: 257
orientation: inline

BBa_I746360
type: SequenceAnnotation
persistent identity: annotation2273091
display id: annotation2273091
version: 1
top level: BBa_K389402
location: https://synbiohub.org/public/igem/BBa_K389402/annotation2273091/range2273091/1
component: BBa_I746360

https://synbiohub.org/public/igem/BBa_K389402/annotation2273091/range2273091/1
type: Range
persistent identity: range2273091
display id: range2273091
version: 1
direction: direction/reverse
top level: BBa_K389402
start: 1115
end: 1205
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2273093
display id: annotation2273093
version: 1
top level: BBa_K389402
location: https://synbiohub.org/public/igem/BBa_K389402/annotation2273093/range2273093/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K389402/annotation2273093/range2273093/1
type: Range
persistent identity: range2273093
display id: range2273093
version: 1
direction: direction/reverse
top level: BBa_K389402
start: 1214
end: 1225
orientation: inline

BBa_E1010
type: SequenceAnnotation
persistent identity: annotation2273082
display id: annotation2273082
version: 1
top level: BBa_K389402
location: https://synbiohub.org/public/igem/BBa_K389402/annotation2273082/range2273082/1
component: BBa_E1010

https://synbiohub.org/public/igem/BBa_K389402/annotation2273082/range2273082/1
type: Range
persistent identity: range2273082
display id: range2273082
version: 1
direction: direction/reverse
top level: BBa_K389402
start: 264
end: 969
orientation: inline

BBa_I712019
type: ComponentDefinition
persistent identity: BBa I712019
display id: BBa_I712019
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Firefly luciferase - luciferase from Photinus pyralis
creator: Anja Korenčič
created: 2007-10-20T11:00:00Z
modified: 2015-08-31T04:07:46Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _130_
m user id: 0
owner id: 1846
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: Firefly luciferase is luciferase from Photinus pyralis. By oxidation of substrate luciferin light is produced. Luciferase thus acts as a reporter protein when connected to other proteins or promoters.
mutable notes: Part is cloned into pSBAK3 vector with terminator.
mutable provenance: This firefly luciferase comes from Promega PGL3 vector.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_I712019_sequence/1

BBa_K216014
type: ComponentDefinition
persistent identity: BBa K216014
display id: BBa_K216014
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PyeaR with Renilla luciferase
creator: Edinburgh iGEM 2009
created: 2009-10-15T11:00:00Z
modified: 2015-05-08T01:11:30Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _317_
m user id: 0
owner id: 837
owning group id: 163
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This is nitrate- and nitrite-responsive promoter PyeaR of ''Escherichia coli'' joined to ''Renilla'' luciferase with the addition of a strong bacterial ribosome binding site. This should lead to production of light in the presence of nitrate/nitrate and coelenterazine.
mutable notes: No special considerations. Sequencing suggests that there is an extra 'A' base following the scar between the RBS and the Renilla luciferase; that is, the scar region reads TACTAGAATG instead of TACTAGATG. If the sequencing is correct, this base must be present in J52008 or have been introduced by a random event during the construction process. This makes the gap between the RBS and start codon one base longer, but still well within the optimal range for E. coli and B. subtilis.
mutable provenance: Made by joining PyeaR BioBrick BBa_K216005 to a PCR fusion of strong synthetic ribosome binding site J15001 and Renilla luciferase BioBrick J52008.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_J52008
component: BBa_J15001
component: BBa_K216005
sequence annotation: BBa_J52008
sequence annotation: BBa_K216005
sequence annotation: BBa_J15001
sequence: https://synbiohub.org/public/igem/BBa_K216014_sequence/1

BBa_J52008
type: Component
persistent identity: component2047079
display id: component2047079
version: 1
top level: BBa_K216014
access: public
definition: BBa_J52008

BBa_J15001
type: Component
persistent identity: component2047076
display id: component2047076
version: 1
top level: BBa_K216014
access: public
definition: https://synbiohub.org/public/igem/BBa_J15001/1

BBa_K216005
type: Component
persistent identity: component2047073
display id: component2047073
version: 1
top level: BBa_K216014
access: public
definition: https://synbiohub.org/public/igem/BBa_K216005/1

BBa_J52008
type: SequenceAnnotation
persistent identity: annotation2047079
display id: annotation2047079
version: 1
top level: BBa_K216014
location: https://synbiohub.org/public/igem/BBa_K216014/annotation2047079/range2047079/1
component: BBa_J52008

https://synbiohub.org/public/igem/BBa_K216014/annotation2047079/range2047079/1
type: Range
persistent identity: range2047079
display id: range2047079
version: 1
direction: direction/reverse
top level: BBa_K216014
start: 125
end: 1060
orientation: inline

BBa_K216005
type: SequenceAnnotation
persistent identity: annotation2047073
display id: annotation2047073
version: 1
top level: BBa_K216014
location: https://synbiohub.org/public/igem/BBa_K216014/annotation2047073/range2047073/1
component: BBa_K216005

https://synbiohub.org/public/igem/BBa_K216014/annotation2047073/range2047073/1
type: Range
persistent identity: range2047073
display id: range2047073
version: 1
direction: direction/reverse
top level: BBa_K216014
start: 1
end: 100
orientation: inline

BBa_J15001
type: SequenceAnnotation
persistent identity: annotation2047076
display id: annotation2047076
version: 1
top level: BBa_K216014
location: https://synbiohub.org/public/igem/BBa_K216014/annotation2047076/range2047076/1
component: BBa_J15001

https://synbiohub.org/public/igem/BBa_K216014/annotation2047076/range2047076/1
type: Range
persistent identity: range2047076
display id: range2047076
version: 1
direction: direction/reverse
top level: BBa_K216014
start: 109
end: 118
orientation: inline

BBa_J52011
type: ComponentDefinition
persistent identity: BBa J52011
display id: BBa_J52011
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: dnMyD88-linker-Rluc
creator: Monika Ciglic
created: 2006-10-13T11:00:00Z
modified: 2015-08-31T03:54:04Z
discontinued: false
dominant: false
group u list: _80_
m user id: 0
owner id: 800
owning group id: 80
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: dnMyD88 is a dominant negative form of protein MyD88 (an important protein in signaling cascade through TLR4 receptor), which was our main target for modifying the signaling path. dnMyD88 is linked to Rluc (which acts as a reporter) through six aminoacid long protein linker.
mutable notes: dnMyD88-linker-Rluc was made by using PCR ligation technique. This part is cloned in BioBrick vector pSB1AK3.
mutable provenance: -
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: dnMyD88
sequence annotation: linker
sequence annotation: dnMyD88-linker-Rluc
sequence annotation: Rluc
sequence: https://synbiohub.org/public/igem/BBa_J52011_sequence/1

dnMyD88
type: SequenceAnnotation
persistent identity: annotation1902834
display id: annotation1902834
version: 1
top level: BBa_J52011
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J52011/annotation1902834/range1902834/1

https://synbiohub.org/public/igem/BBa_J52011/annotation1902834/range1902834/1
type: Range
persistent identity: range1902834
display id: range1902834
version: 1
direction: direction/forward
top level: BBa_J52011
start: 1
end: 417
orientation: inline

linker
type: SequenceAnnotation
persistent identity: annotation1902835
display id: annotation1902835
version: 1
top level: BBa_J52011
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_J52011/annotation1902835/range1902835/1

https://synbiohub.org/public/igem/BBa_J52011/annotation1902835/range1902835/1
type: Range
persistent identity: range1902835
display id: range1902835
version: 1
direction: direction/forward
top level: BBa_J52011
start: 418
end: 435
orientation: inline

dnMyD88-linker-Rluc
type: SequenceAnnotation
persistent identity: annotation1902846
display id: annotation1902846
version: 1
top level: BBa_J52011
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_J52011/annotation1902846/range1902846/1

https://synbiohub.org/public/igem/BBa_J52011/annotation1902846/range1902846/1
type: Range
persistent identity: range1902846
display id: range1902846
version: 1
direction: direction/forward
top level: BBa_J52011
start: 1
end: 1371
orientation: inline

Rluc
type: SequenceAnnotation
persistent identity: annotation1902836
display id: annotation1902836
version: 1
top level: BBa_J52011
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_J52011/annotation1902836/range1902836/1

https://synbiohub.org/public/igem/BBa_J52011/annotation1902836/range1902836/1
type: Range
persistent identity: range1902836
display id: range1902836
version: 1
direction: direction/forward
top level: BBa_J52011
start: 436
end: 1371
orientation: inline

BBa_K1689001
type: ComponentDefinition
persistent identity: BBa K1689001
display id: BBa_K1689001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Coding sequence of STV-N-luc
creator: ZHANG Yihao
created: 2015-09-15T11:00:00Z
modified: 2016-01-19T02:50:55Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2107_
m user id: 4206
owner id: 26464
owning group id: 9
status: status/Planning
bookmark: false
mutable description: including streptavidin, 2??linker, and firefly luciferase N terminal 416 amino acid domain.
mutable notes: streptavidin and N terminal 416 amino acid domain was linked by 2??linker
mutable provenance: E.coli
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: streptavidin
sequence annotation: random spacer
sequence annotation: linker
sequence annotation: N416 terminal of firefly luciferase
sequence annotation: stop codon
sequence annotation: start codon
sequence: https://synbiohub.org/public/igem/BBa_K1689001_sequence/1

streptavidin
type: SequenceAnnotation
persistent identity: annotation2469930
display id: annotation2469930
version: 1
top level: BBa_K1689001
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1689001/annotation2469930/range2469930/1

https://synbiohub.org/public/igem/BBa_K1689001/annotation2469930/range2469930/1
type: Range
persistent identity: range2469930
display id: range2469930
version: 1
direction: direction/forward
top level: BBa_K1689001
start: 38
end: 391
orientation: inline

random spacer
type: SequenceAnnotation
persistent identity: annotation2477762
display id: annotation2477762
version: 1
top level: BBa_K1689001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1689001/annotation2477762/range2477762/1

https://synbiohub.org/public/igem/BBa_K1689001/annotation2477762/range2477762/1
type: Range
persistent identity: range2477762
display id: range2477762
version: 1
direction: direction/forward
top level: BBa_K1689001
start: 1673
end: 1727
orientation: inline

linker
type: SequenceAnnotation
persistent identity: annotation2469931
display id: annotation2469931
version: 1
top level: BBa_K1689001
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1689001/annotation2469931/range2469931/1

https://synbiohub.org/public/igem/BBa_K1689001/annotation2469931/range2469931/1
type: Range
persistent identity: range2469931
display id: range2469931
version: 1
direction: direction/forward
top level: BBa_K1689001
start: 392
end: 421
orientation: inline

N416 terminal of firefly luciferase
type: SequenceAnnotation
persistent identity: annotation2469932
display id: annotation2469932
version: 1
top level: BBa_K1689001
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1689001/annotation2469932/range2469932/1

https://synbiohub.org/public/igem/BBa_K1689001/annotation2469932/range2469932/1
type: Range
persistent identity: range2469932
display id: range2469932
version: 1
direction: direction/forward
top level: BBa_K1689001
start: 422
end: 1669
orientation: inline

stop codon
type: SequenceAnnotation
persistent identity: annotation2469934
display id: annotation2469934
version: 1
top level: BBa_K1689001
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1689001/annotation2469934/range2469934/1

https://synbiohub.org/public/igem/BBa_K1689001/annotation2469934/range2469934/1
type: Range
persistent identity: range2469934
display id: range2469934
version: 1
direction: direction/forward
top level: BBa_K1689001
start: 1670
end: 1672
orientation: inline

random spacer
type: SequenceAnnotation
persistent identity: annotation2477761
display id: annotation2477761
version: 1
top level: BBa_K1689001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1689001/annotation2477761/range2477761/1

https://synbiohub.org/public/igem/BBa_K1689001/annotation2477761/range2477761/1
type: Range
persistent identity: range2477761
display id: range2477761
version: 1
direction: direction/forward
top level: BBa_K1689001
start: 1
end: 34
orientation: inline

start codon
type: SequenceAnnotation
persistent identity: annotation2469928
display id: annotation2469928
version: 1
top level: BBa_K1689001
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1689001/annotation2469928/range2469928/1

https://synbiohub.org/public/igem/BBa_K1689001/annotation2469928/range2469928/1
type: Range
persistent identity: range2469928
display id: range2469928
version: 1
direction: direction/forward
top level: BBa_K1689001
start: 35
end: 37
orientation: inline

BBa_K1486027
type: ComponentDefinition
persistent identity: BBa K1486027
display id: BBa_K1486027
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: R.reniformis luciferase + ADH1 terminator + Kanamycin resistance
creator: EPFL iGem team 2014
created: 2014-09-26T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: To edit
mutable notes: To edit
mutable provenance: To edit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000324
role: partType/Tag
component: BBa_K1486037
component: BBa_K1486024
component: BBa_K1486019
component: BBa_K1486020
component: BBa_K801012
sequence annotation: BBa_K1486020
sequence annotation: BBa_K1486019
sequence annotation: BBa_K801012
sequence annotation: BBa_K1486037
sequence annotation: BBa_K1486024
sequence: https://synbiohub.org/public/igem/BBa_K1486027_sequence/1

BBa_K1486037
type: Component
persistent identity: component2417706
display id: component2417706
version: 1
top level: BBa_K1486027
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486037/1

BBa_K1486024
type: Component
persistent identity: component2417714
display id: component2417714
version: 1
top level: BBa_K1486027
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486024/1

BBa_K1486019
type: Component
persistent identity: component2417707
display id: component2417707
version: 1
top level: BBa_K1486027
access: public
definition: BBa_K1486019

BBa_K1486020
type: Component
persistent identity: component2417708
display id: component2417708
version: 1
top level: BBa_K1486027
access: public
definition: BBa_K1486020

BBa_K801012
type: Component
persistent identity: component2417710
display id: component2417710
version: 1
top level: BBa_K1486027
access: public
definition: https://synbiohub.org/public/igem/BBa_K801012/1

BBa_K1486020
type: SequenceAnnotation
persistent identity: annotation2417708
display id: annotation2417708
version: 1
top level: BBa_K1486027
location: https://synbiohub.org/public/igem/BBa_K1486027/annotation2417708/range2417708/1
component: BBa_K1486020

https://synbiohub.org/public/igem/BBa_K1486027/annotation2417708/range2417708/1
type: Range
persistent identity: range2417708
display id: range2417708
version: 1
direction: direction/reverse
top level: BBa_K1486027
start: 370
end: 975
orientation: inline

BBa_K1486019
type: SequenceAnnotation
persistent identity: annotation2417707
display id: annotation2417707
version: 1
top level: BBa_K1486027
location: https://synbiohub.org/public/igem/BBa_K1486027/annotation2417707/range2417707/1
component: BBa_K1486019

https://synbiohub.org/public/igem/BBa_K1486027/annotation2417707/range2417707/1
type: Range
persistent identity: range2417707
display id: range2417707
version: 1
direction: direction/reverse
top level: BBa_K1486027
start: 40
end: 369
orientation: inline

BBa_K801012
type: SequenceAnnotation
persistent identity: annotation2417710
display id: annotation2417710
version: 1
top level: BBa_K1486027
location: https://synbiohub.org/public/igem/BBa_K1486027/annotation2417710/range2417710/1
component: BBa_K801012

https://synbiohub.org/public/igem/BBa_K1486027/annotation2417710/range2417710/1
type: Range
persistent identity: range2417710
display id: range2417710
version: 1
direction: direction/reverse
top level: BBa_K1486027
start: 976
end: 1324
orientation: inline

BBa_K1486037
type: SequenceAnnotation
persistent identity: annotation2417706
display id: annotation2417706
version: 1
top level: BBa_K1486027
location: https://synbiohub.org/public/igem/BBa_K1486027/annotation2417706/range2417706/1
component: BBa_K1486037

https://synbiohub.org/public/igem/BBa_K1486027/annotation2417706/range2417706/1
type: Range
persistent identity: range2417706
display id: range2417706
version: 1
direction: direction/reverse
top level: BBa_K1486027
start: 1
end: 39
orientation: inline

BBa_K1486024
type: SequenceAnnotation
persistent identity: annotation2417714
display id: annotation2417714
version: 1
top level: BBa_K1486027
location: https://synbiohub.org/public/igem/BBa_K1486027/annotation2417714/range2417714/1
component: BBa_K1486024

https://synbiohub.org/public/igem/BBa_K1486027/annotation2417714/range2417714/1
type: Range
persistent identity: range2417714
display id: range2417714
version: 1
direction: direction/reverse
top level: BBa_K1486027
start: 1325
end: 2681
orientation: inline

BBa_K1689006
type: ComponentDefinition
persistent identity: BBa K1689006
display id: BBa_K1689006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Coding sequence of FKBP-Cluc394
creator: Xiang Li
created: 2015-09-15T11:00:00Z
modified: 2016-01-19T01:28:16Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2107_
m user id: 4206
owner id: 26464
owning group id: 9
status: status/Planning
bookmark: false
mutable description: including FKBP, 2??linker, and firefly luciferase C terminal 394 amino acid domain.
mutable notes: FKBP and C terminal 394 amino acid domain are linked by 2??linker
mutable provenance: E.coli
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: FKBP
sequence annotation: start codon
sequence annotation: J23110
sequence annotation: B0034
sequence annotation: stop codon
sequence annotation: C394 terminal domain of firefly luciferase
sequence annotation: GGGGSGGGGS linker
sequence: https://synbiohub.org/public/igem/BBa_K1689006_sequence/1

FKBP
type: SequenceAnnotation
persistent identity: annotation2470150
display id: annotation2470150
version: 1
top level: BBa_K1689006
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1689006/annotation2470150/range2470150/1

https://synbiohub.org/public/igem/BBa_K1689006/annotation2470150/range2470150/1
type: Range
persistent identity: range2470150
display id: range2470150
version: 1
direction: direction/forward
top level: BBa_K1689006
start: 51
end: 371
orientation: inline

start codon
type: SequenceAnnotation
persistent identity: annotation2470149
display id: annotation2470149
version: 1
top level: BBa_K1689006
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1689006/annotation2470149/range2470149/1

https://synbiohub.org/public/igem/BBa_K1689006/annotation2470149/range2470149/1
type: Range
persistent identity: range2470149
display id: range2470149
version: 1
direction: direction/forward
top level: BBa_K1689006
start: 48
end: 50
orientation: inline

J23110
type: SequenceAnnotation
persistent identity: annotation2470146
display id: annotation2470146
version: 1
top level: BBa_K1689006
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1689006/annotation2470146/range2470146/1

https://synbiohub.org/public/igem/BBa_K1689006/annotation2470146/range2470146/1
type: Range
persistent identity: range2470146
display id: range2470146
version: 1
direction: direction/forward
top level: BBa_K1689006
start: 1
end: 35
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation2470147
display id: annotation2470147
version: 1
top level: BBa_K1689006
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1689006/annotation2470147/range2470147/1

https://synbiohub.org/public/igem/BBa_K1689006/annotation2470147/range2470147/1
type: Range
persistent identity: range2470147
display id: range2470147
version: 1
direction: direction/forward
top level: BBa_K1689006
start: 36
end: 47
orientation: inline

stop codon
type: SequenceAnnotation
persistent identity: annotation2470155
display id: annotation2470155
version: 1
top level: BBa_K1689006
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1689006/annotation2470155/range2470155/1

https://synbiohub.org/public/igem/BBa_K1689006/annotation2470155/range2470155/1
type: Range
persistent identity: range2470155
display id: range2470155
version: 1
direction: direction/forward
top level: BBa_K1689006
start: 873
end: 875
orientation: inline

C394 terminal domain of firefly luciferase
type: SequenceAnnotation
persistent identity: annotation2470154
display id: annotation2470154
version: 1
top level: BBa_K1689006
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1689006/annotation2470154/range2470154/1

https://synbiohub.org/public/igem/BBa_K1689006/annotation2470154/range2470154/1
type: Range
persistent identity: range2470154
display id: range2470154
version: 1
direction: direction/forward
top level: BBa_K1689006
start: 402
end: 872
orientation: inline

GGGGSGGGGS linker
type: SequenceAnnotation
persistent identity: annotation2470152
display id: annotation2470152
version: 1
top level: BBa_K1689006
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1689006/annotation2470152/range2470152/1

https://synbiohub.org/public/igem/BBa_K1689006/annotation2470152/range2470152/1
type: Range
persistent identity: range2470152
display id: range2470152
version: 1
direction: direction/forward
top level: BBa_K1689006
start: 372
end: 401
orientation: inline

BBa_K1761006
type: ComponentDefinition
persistent identity: BBa K1761006
display id: BBa_K1761006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: SmallBit Split Luciferase
creator: Laura van Smeden
created: 2015-09-16T11:00:00Z
modified: 2016-01-25T01:15:19Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2183_
m user id: 4206
owner id: 25572
owning group id: 9
status: status/Planning
bookmark: false
mutable description: SmallBit is the small part of a Split Luciferase. Its molecular weight is 1 kDa. This construct on its own has no function since SmallBit on itself has no luminescence activity. For luminescence activity, the LargeBit of the Split Luciferace is needed.
mutable notes: The given sequence from the company is used
mutable provenance: The company Promega
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316

BBa_K424003
type: ComponentDefinition
persistent identity: BBa K424003
display id: BBa_K424003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: luxC for light production protein generator
creator: Patrick Nee
created: 2010-06-06T11:00:00Z
modified: 2015-05-08T01:12:28Z
discontinued: true
dominant: false
experience: experience/None
group u list: _545_
m user id: 0
owner id: 6272
owning group id: 9
sample status: Discontinued
status: status/Deleted
bookmark: false
mutable description: What does the device do? Helps to produce light What are the inputs and outputs to the device? Takes in a long-chain aldehyde + CoA + NADP(+) and produces a long-chain acyl-CoA + NADPH. Are any other devices needed? Also requires the other genes of the bacterial luciferase pathway. Does the device have any chassis dependencies?
mutable notes: This device uses a strong RBS to drive translation of luxC.
mutable provenance: Uses part <partinfo>BBa_K424002</partinfo>
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0030
component: BBa_K424002
sequence annotation: BBa_K424002
sequence annotation: BBa_B0030
sequence: https://synbiohub.org/public/igem/BBa_K424003_sequence/1

BBa_B0030
type: Component
persistent identity: component2070296
display id: component2070296
version: 1
top level: BBa_K424003
access: public
definition: https://synbiohub.org/public/igem/BBa_B0030/1

BBa_K424002
type: Component
persistent identity: component2070301
display id: component2070301
version: 1
top level: BBa_K424003
access: public
definition: https://synbiohub.org/public/igem/BBa_K424002/1

BBa_K424002
type: SequenceAnnotation
persistent identity: annotation2070301
display id: annotation2070301
version: 1
top level: BBa_K424003
location: https://synbiohub.org/public/igem/BBa_K424003/annotation2070301/range2070301/1
component: BBa_K424002

https://synbiohub.org/public/igem/BBa_K424003/annotation2070301/range2070301/1
type: Range
persistent identity: range2070301
display id: range2070301
version: 1
direction: direction/reverse
top level: BBa_K424003
start: 24
end: 272
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation2070296
display id: annotation2070296
version: 1
top level: BBa_K424003
location: https://synbiohub.org/public/igem/BBa_K424003/annotation2070296/range2070296/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_K424003/annotation2070296/range2070296/1
type: Range
persistent identity: range2070296
display id: range2070296
version: 1
direction: direction/forward
top level: BBa_K424003
start: 1
end: 15
orientation: inline

BBa_K819000
type: ComponentDefinition
persistent identity: BBa K819000
display id: BBa_K819000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Luminescence-responsive Promoter+GFP(BBa_E0840)
creator: Haoqian Zhang
created: 2012-08-30T11:00:00Z
modified: 2015-05-08T01:13:29Z
discontinued: true
dominant: false
experience: experience/Works
group u list: _1077_
m user id: 0
owner id: 4411
owning group id: 9
sample status: Discontinued
status: status/Deleted
bookmark: false
mutable description: The luminescence-responsive promoter was engineered from promoter ColE7, during which the LexA binding site was mutated to guarantee that the endogenous LexA will not disturb the function of this promoter. This mutated LexA binding site within luminescence-responsive promoter can be recognized by corresponding mutated LexA DNA binding domain.
mutable notes: The LexA binding site was mutated to guarantee that the endogenous LexA will not disturb the function of this promoter. This mutated LexA binding site within luminescence-responsive promoter can be recognized by corresponding mutated LexA DNA binding domain.
mutable provenance: ColE7 promoter, ColE plasmid
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: LexA408 DNA binding domain
sequence: https://synbiohub.org/public/igem/BBa_K819000_sequence/1

LexA408 DNA binding domain
type: SequenceAnnotation
persistent identity: annotation2181589
display id: annotation2181589
version: 1
top level: BBa_K819000
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K819000/annotation2181589/range2181589/1

https://synbiohub.org/public/igem/BBa_K819000/annotation2181589/range2181589/1
type: Range
persistent identity: range2181589
display id: range2181589
version: 1
direction: direction/forward
top level: BBa_K819000
start: 1
end: 4
orientation: inline

BBa_K389318
type: ComponentDefinition
persistent identity: BBa K389318
display id: BBa_K389318
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: strong constitutive promoter + luc
creator: Jonas Aretz
created: 2010-10-11T11:00:00Z
modified: 2015-05-08T01:12:19Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _517_
m user id: 0
owner id: 6670
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This is a firefly luciferase gene under the control of a strong constitutive promoter.
mutable notes: strong promoter, strong RBS, used this to demonstrate that our luciferase BioBrick is working
mutable provenance: * promoter and RBS from partsregistry * luciferase from pGL4.10luc2 (Promega)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K389004
component: BBa_J23102
component: BBa_B0034
sequence annotation: BBa_J23102
sequence annotation: BBa_B0034
sequence annotation: BBa_K389004
sequence: https://synbiohub.org/public/igem/BBa_K389318_sequence/1

BBa_K389004
type: Component
persistent identity: component2085590
display id: component2085590
version: 1
top level: BBa_K389318
access: public
definition: luc

BBa_J23102
type: Component
persistent identity: component2085585
display id: component2085585
version: 1
top level: BBa_K389318
access: public
definition: https://synbiohub.org/public/igem/BBa_J23102/1

BBa_B0034
type: Component
persistent identity: component2085587
display id: component2085587
version: 1
top level: BBa_K389318
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_J23102
type: SequenceAnnotation
persistent identity: annotation2085585
display id: annotation2085585
version: 1
top level: BBa_K389318
location: https://synbiohub.org/public/igem/BBa_K389318/annotation2085585/range2085585/1
component: BBa_J23102

https://synbiohub.org/public/igem/BBa_K389318/annotation2085585/range2085585/1
type: Range
persistent identity: range2085585
display id: range2085585
version: 1
direction: direction/reverse
top level: BBa_K389318
start: 1
end: 35
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2085587
display id: annotation2085587
version: 1
top level: BBa_K389318
location: https://synbiohub.org/public/igem/BBa_K389318/annotation2085587/range2085587/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K389318/annotation2085587/range2085587/1
type: Range
persistent identity: range2085587
display id: range2085587
version: 1
direction: direction/reverse
top level: BBa_K389318
start: 44
end: 55
orientation: inline

BBa_K389004
type: SequenceAnnotation
persistent identity: annotation2085590
display id: annotation2085590
version: 1
top level: BBa_K389318
location: https://synbiohub.org/public/igem/BBa_K389318/annotation2085590/range2085590/1
component: BBa_K389004

https://synbiohub.org/public/igem/BBa_K389318/annotation2085590/range2085590/1
type: Range
persistent identity: range2085590
display id: range2085590
version: 1
direction: direction/reverse
top level: BBa_K389318
start: 62
end: 1717
orientation: inline