//function/reporter/fluorescence
type: Collection
persistent identity: function reporter fluorescence
display id: function_reporter_fluorescence
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
owned by: james
owned by: myers
member: BBa_K863122
member: BBa_K1399016
member: mCherry
member: BBa_K1740000
member: CspB | RFP
member: BBa_K1031224
member: BBa_K1486034
member: BBa_K1673103
member: dTomato
member: BBa_K1033915
member: BBa_K1899002
member: BBa_K1072000
member: BBa_K180012
member: mRFP1
member: BBa_K191004
member: BBa_K216011
member: BBa_K389052
member: BBa_J97002
member: BBa_K1680019
member: RD29A + Ko
member: GFP
member: BBa_K1022100
member: BBa_K1020012
member: BBa_K1679006
member: eYFP-GOI
member: SgsE | CFP
member: BBa_K1321356
member: BBa_K1980010
member: BBa_K1486029
member: BBa_K106005
member: BBa_K777133
member: BBa_K1680024
member: BBa_K819007
member: BBa_K1486040
member: RFP2
member: BBa_K1486010
member: Tm-nCFP
member: CFP
member: BBa_K819006
member: BBa_K1468002
member: BBa_K157005
member: GFP-TS
member: BBa_K1333204
member: BBa_K180006
member: BBa_K180019
member: SP-Lipocal
member: RFP1
member: eGFP-PTS
member: sfCFP
member: mRFP1-NLS
member: BBa_K1680025
member: BBa_K249017
member: BBa_K1031302
member: BBa_K1185001
member: BBa_K1468001
member: BBa_K294055
member: BBa_K106028
member: BBa_K157006
member: BBa_K1031521
member: PlsirE5501
member: BBa_K1673102
member: BBa_K389016
member: BBa_K1491004
member: mOrange
member: no ATG GFP
member: GFP_His
member: BBa_K1031803
member: GFP-Si4Tag
member: BBa_K1673104

ecfp
type: ComponentDefinition
persistent identity: BBa I15017
display id: BBa_I15017
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: B0032.EYFP
creator: Jeff Tabor
created: 2005-04-14T11:00:00Z
modified: 2015-08-31T04:07:38Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _5_
m user id: 0
owner id: 88
owning group id: 5
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This part accepts POPS to generate EYFP under the medium strength RBS B0032.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_E0030
component: BBa_B0032
sequence annotation: BBa_B0032
sequence annotation: BBa_E0030
sequence: https://synbiohub.org/public/igem/BBa_I15017_sequence/1

BBa_E0030
type: Component
persistent identity: component1478422
display id: component1478422
version: 1
top level: ecfp
access: public
definition: eyfp

BBa_B0032
type: Component
persistent identity: component1478419
display id: component1478419
version: 1
top level: ecfp
access: public
definition: https://synbiohub.org/public/igem/BBa_B0032/1

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation1478419
display id: annotation1478419
version: 1
top level: ecfp
location: https://synbiohub.org/public/igem/BBa_I15017/annotation1478419/range1478419/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_I15017/annotation1478419/range1478419/1
type: Range
persistent identity: range1478419
display id: range1478419
version: 1
direction: direction/forward
top level: ecfp
start: 1
end: 13
orientation: inline

BBa_E0030
type: SequenceAnnotation
persistent identity: annotation1478422
display id: annotation1478422
version: 1
top level: ecfp
location: https://synbiohub.org/public/igem/BBa_I15017/annotation1478422/range1478422/1
component: BBa_E0030

https://synbiohub.org/public/igem/BBa_I15017/annotation1478422/range1478422/1
type: Range
persistent identity: range1478422
display id: range1478422
version: 1
direction: direction/forward
top level: ecfp
start: 20
end: 742
orientation: inline

SP-AntiNIP
type: ComponentDefinition
persistent identity: BBa K157026
display id: BBa_K157026
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: SP-AntiNIP-GGGSlinker-Tm-Fluolinker-cYFP
creator: iGEM Team Freiburg 2008
created: 2008-10-25T11:00:00Z
modified: 2015-05-08T01:10:55Z
discontinued: false
dominant: false
experience: experience/None
group u list: _232_
m user id: 0
owner id: 1673
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: A composite part consisting of the signal peptide of Erbb1/EGF-receptor, scFV-antiNIP, GGGSflexlinker (extracellular part), the trans-membrane region of the Erbb1/EGF-receptor and the C-terminal part of the split fluorophor YFP with a fluolinker joined to its N-Terminus (intracellular part).
mutable notes: none
mutable provenance: Derived by cloning of the parts BBa_K157001, BBa_K157003, BBa_K157013, BBa_K157002, BBa_K157009 and BBa_K157007. For further information see the description of the parts mentioned above.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: GGGS-Linker
sequence annotation: Scar (Thr, Gly)
sequence annotation: SP-AntiNIP-GGGSlinker-Tm-Fluolinker-cYFP
sequence annotation: C-Term_YFP
sequence annotation: Fluolinker
sequence annotation: Sc-Fv_anti-NIP
sequence annotation: SP
sequence annotation: Tm
sequence annotation: NgoMI
sequence annotation: AgeI
sequence: https://synbiohub.org/public/igem/BBa_K157026_sequence/1

GGGS-Linker
type: SequenceAnnotation
persistent identity: annotation1992642
display id: annotation1992642
version: 1
top level: SP-AntiNIP
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157026/annotation1992642/range1992642/1

https://synbiohub.org/public/igem/BBa_K157026/annotation1992642/range1992642/1
type: Range
persistent identity: range1992642
display id: range1992642
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 829
end: 873
orientation: inline

Scar (Thr, Gly)
type: SequenceAnnotation
persistent identity: annotation1992641
display id: annotation1992641
version: 1
top level: SP-AntiNIP
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157026/annotation1992641/range1992641/1

https://synbiohub.org/public/igem/BBa_K157026/annotation1992641/range1992641/1
type: Range
persistent identity: range1992641
display id: range1992641
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 823
end: 828
orientation: inline

SP-AntiNIP-GGGSlinker-Tm-Fluolinker-cYFP
type: SequenceAnnotation
persistent identity: annotation1990176
display id: annotation1990176
version: 1
top level: SP-AntiNIP
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K157026/annotation1990176/range1990176/1

https://synbiohub.org/public/igem/BBa_K157026/annotation1990176/range1990176/1
type: Range
persistent identity: range1990176
display id: range1990176
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 1
end: 1260
orientation: inline

Scar (Thr, Gly)
type: SequenceAnnotation
persistent identity: annotation1992645
display id: annotation1992645
version: 1
top level: SP-AntiNIP
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157026/annotation1992645/range1992645/1

https://synbiohub.org/public/igem/BBa_K157026/annotation1992645/range1992645/1
type: Range
persistent identity: range1992645
display id: range1992645
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 949
end: 954
orientation: inline

C-Term_YFP
type: SequenceAnnotation
persistent identity: annotation1992648
display id: annotation1992648
version: 1
top level: SP-AntiNIP
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157026/annotation1992648/range1992648/1

https://synbiohub.org/public/igem/BBa_K157026/annotation1992648/range1992648/1
type: Range
persistent identity: range1992648
display id: range1992648
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 1012
end: 1254
orientation: inline

Scar (Thr, Gly)
type: SequenceAnnotation
persistent identity: annotation1992639
display id: annotation1992639
version: 1
top level: SP-AntiNIP
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157026/annotation1992639/range1992639/1

https://synbiohub.org/public/igem/BBa_K157026/annotation1992639/range1992639/1
type: Range
persistent identity: range1992639
display id: range1992639
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 82
end: 87
orientation: inline

Fluolinker
type: SequenceAnnotation
persistent identity: annotation1992646
display id: annotation1992646
version: 1
top level: SP-AntiNIP
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157026/annotation1992646/range1992646/1

https://synbiohub.org/public/igem/BBa_K157026/annotation1992646/range1992646/1
type: Range
persistent identity: range1992646
display id: range1992646
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 955
end: 1005
orientation: inline

Sc-Fv_anti-NIP
type: SequenceAnnotation
persistent identity: annotation1992640
display id: annotation1992640
version: 1
top level: SP-AntiNIP
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157026/annotation1992640/range1992640/1

https://synbiohub.org/public/igem/BBa_K157026/annotation1992640/range1992640/1
type: Range
persistent identity: range1992640
display id: range1992640
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 88
end: 822
orientation: inline

SP
type: SequenceAnnotation
persistent identity: annotation1992638
display id: annotation1992638
version: 1
top level: SP-AntiNIP
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157026/annotation1992638/range1992638/1

https://synbiohub.org/public/igem/BBa_K157026/annotation1992638/range1992638/1
type: Range
persistent identity: range1992638
display id: range1992638
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 10
end: 81
orientation: inline

Tm
type: SequenceAnnotation
persistent identity: annotation1992644
display id: annotation1992644
version: 1
top level: SP-AntiNIP
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157026/annotation1992644/range1992644/1

https://synbiohub.org/public/igem/BBa_K157026/annotation1992644/range1992644/1
type: Range
persistent identity: range1992644
display id: range1992644
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 880
end: 948
orientation: inline

Scar (Thr, Gly)
type: SequenceAnnotation
persistent identity: annotation1992647
display id: annotation1992647
version: 1
top level: SP-AntiNIP
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157026/annotation1992647/range1992647/1

https://synbiohub.org/public/igem/BBa_K157026/annotation1992647/range1992647/1
type: Range
persistent identity: range1992647
display id: range1992647
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 1006
end: 1011
orientation: inline

NgoMI
type: SequenceAnnotation
persistent identity: annotation1990174
display id: annotation1990174
version: 1
top level: SP-AntiNIP
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157026/annotation1990174/range1990174/1

https://synbiohub.org/public/igem/BBa_K157026/annotation1990174/range1990174/1
type: Range
persistent identity: range1990174
display id: range1990174
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 4
end: 9
orientation: inline

AgeI
type: SequenceAnnotation
persistent identity: annotation1990175
display id: annotation1990175
version: 1
top level: SP-AntiNIP
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157026/annotation1990175/range1990175/1

https://synbiohub.org/public/igem/BBa_K157026/annotation1990175/range1990175/1
type: Range
persistent identity: range1990175
display id: range1990175
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 1255
end: 1260
orientation: inline

Scar (Thr, Gly)
type: SequenceAnnotation
persistent identity: annotation1992643
display id: annotation1992643
version: 1
top level: SP-AntiNIP
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157026/annotation1992643/range1992643/1

https://synbiohub.org/public/igem/BBa_K157026/annotation1992643/range1992643/1
type: Range
persistent identity: range1992643
display id: range1992643
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 874
end: 879
orientation: inline

BBa_K1857000
type: ComponentDefinition
persistent identity: BBa K1857000
display id: BBa_K1857000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: mVenus
creator: Joshua Timmons
created: 2015-09-15T11:00:00Z
modified: 2016-01-25T01:12:03Z
discontinued: false
dominant: false
group u list: _2285_
m user id: 4206
owner id: 26495
owning group id: 9
status: status/Planning
bookmark: false
mutable description: mVenus is a Chlamydomonas reinhardtii nuclear codon-optimized fluorescent protein. A variant of YFP, mVenus excites at 514nm and excites between 520 and 550nm. It includes two introns from RBCS2 in increase expression levels. Lauersen, Kyle., Kruse, Olaf., & Mussgnug Jan. "Targeted expression of nuclear transgenes in Chlamydomonas reinhardtii with a versatile, modular vector toolkit." Applied Microbiology Biotechnology. (2015) 99;3491-3503.
mutable notes: N/A
mutable provenance: This part was adapted from pOopt_mVenus_Hyg and was originally from ChlamyCollection.org
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: mVenus
sequence: https://synbiohub.org/public/igem/BBa_K1857000_sequence/1

mVenus
type: SequenceAnnotation
persistent identity: annotation2473680
display id: annotation2473680
version: 1
top level: BBa_K1857000
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1857000/annotation2473680/range2473680/1

https://synbiohub.org/public/igem/BBa_K1857000/annotation2473680/range2473680/1
type: Range
persistent identity: range2473680
display id: range2473680
version: 1
direction: direction/forward
top level: BBa_K1857000
start: 144
end: 1236
orientation: inline

mCherry
type: ComponentDefinition
persistent identity: BBa K678020
display id: BBa_K678020
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: mCherry-module
creator: DTU-Danmark-2
created: 2011-09-12T11:00:00Z
modified: 2015-05-08T01:13:04Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _882_
m user id: 0
owner id: 10556
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part was designed for the Plug'n'Play with DNA assembly standard (RFC 80), therefore this part contains tails at the 5' and 3' ends as shown on the figure below.
mutable notes: No
mutable provenance: The Danish Canser Society
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: mCherry
sequence annotation: Kozak
sequence annotation: Linker 2
sequence: https://synbiohub.org/public/igem/BBa_K678020_sequence/1

mCherry
type: SequenceAnnotation
persistent identity: annotation2127761
display id: annotation2127761
version: 1
top level: mCherry
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K678020/annotation2127761/range2127761/1

https://synbiohub.org/public/igem/BBa_K678020/annotation2127761/range2127761/1
type: Range
persistent identity: range2127761
display id: range2127761
version: 1
direction: direction/forward
top level: mCherry
start: 16
end: 726
orientation: inline

Kozak
type: SequenceAnnotation
persistent identity: annotation2127778
display id: annotation2127778
version: 1
top level: mCherry
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678020/annotation2127778/range2127778/1

https://synbiohub.org/public/igem/BBa_K678020/annotation2127778/range2127778/1
type: Range
persistent identity: range2127778
display id: range2127778
version: 1
direction: direction/forward
top level: mCherry
start: 9
end: 19
orientation: inline

Linker 2
type: SequenceAnnotation
persistent identity: annotation2127760
display id: annotation2127760
version: 1
top level: mCherry
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678020/annotation2127760/range2127760/1

https://synbiohub.org/public/igem/BBa_K678020/annotation2127760/range2127760/1
type: Range
persistent identity: range2127760
display id: range2127760
version: 1
direction: direction/forward
top level: mCherry
start: 1
end: 8
orientation: inline

BBa_K1486028
type: ComponentDefinition
persistent identity: BBa K1486028
display id: BBa_K1486028
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Yeast optimized sfGFP N-terminus (1-214)
creator: EPFL iGem team 2014
created: 2014-09-26T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: split N term of sfGFP To edit
mutable notes: To edit
mutable provenance: To edit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000324
role: partType/Tag
sequence annotation: yeast optimized sfGFP N-terminus
sequence: https://synbiohub.org/public/igem/BBa_K1486028_sequence/1

yeast optimized sfGFP N-terminus
type: SequenceAnnotation
persistent identity: annotation2417081
display id: annotation2417081
version: 1
top level: BBa_K1486028
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1486028/annotation2417081/range2417081/1

https://synbiohub.org/public/igem/BBa_K1486028/annotation2417081/range2417081/1
type: Range
persistent identity: range2417081
display id: range2417081
version: 1
direction: direction/forward
top level: BBa_K1486028
start: 1
end: 642
orientation: inline

BBa_K1673102
type: ComponentDefinition
persistent identity: BBa K1673102
display id: BBa_K1673102
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: UV Mutation Optimized RFP (Improved Expression)
creator: Jarrod Shilts
created: 2015-09-16T11:00:00Z
modified: 2016-01-21T04:04:00Z
discontinued: false
dominant: false
group u list: _2091_
m user id: 4206
owner id: 19415
owning group id: 9
status: status/Planning
bookmark: false
mutable description: RFP sequence, based on E1010, with the number of possible pyrimidine dimer-forming sites reduced to the maximum possible extent. Altered from BBa_K1673101 to increase effective expression approximately 40%
mutable notes: Sequence produced by the Vanderbilt iGEM 2015 mutation optimization software.
mutable provenance: Synthesized DNA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1673102_sequence/1

SP-Lipocal
type: ComponentDefinition
persistent identity: BBa K157037
display id: BBa_K157037
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: SP-Lipocalin-GGGSlinker-Tm-nCFP
creator: iGEM Team Freiburg 2008
created: 2008-10-25T11:00:00Z
modified: 2015-05-08T01:10:55Z
discontinued: false
dominant: false
experience: experience/None
group u list: _232_
m user id: 0
owner id: 1673
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: A composite part consisting of the signal peptide of Erbb1/EGF-receptor, scFV-antiNIP, GGGSflexlinker (extracellular part), the trans-membrane region of the Erbb1/EGF-receptor and the C-terminal part of the split fluorophor YFP with a fluolinker joined to its N-Terminus (intracellular part).
mutable notes: none
mutable provenance: Derived by cloning of the parts BBa_K157001, BBa_K157004, BBa_K157013, BBa_K157002, BBa_K157006. For further information see the description of the parts mentioned above.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: GGGS-Linker
sequence annotation: NgoMI
sequence annotation: Scar (Thr, Gly)
sequence annotation: SP
sequence annotation: Lipocalin
sequence annotation: SP-Lipocalin-GGGSlinker-Tm-nCFP
sequence annotation: AgeI
sequence annotation: Tm
sequence annotation: N-Term_CFP
sequence: https://synbiohub.org/public/igem/BBa_K157037_sequence/1

GGGS-Linker
type: SequenceAnnotation
persistent identity: annotation1993413
display id: annotation1993413
version: 1
top level: SP-Lipocal
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157037/annotation1993413/range1993413/1

https://synbiohub.org/public/igem/BBa_K157037/annotation1993413/range1993413/1
type: Range
persistent identity: range1993413
display id: range1993413
version: 1
direction: direction/forward
top level: SP-Lipocal
start: 616
end: 660
orientation: inline

NgoMI
type: SequenceAnnotation
persistent identity: annotation1990297
display id: annotation1990297
version: 1
top level: SP-Lipocal
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157037/annotation1990297/range1990297/1

https://synbiohub.org/public/igem/BBa_K157037/annotation1990297/range1990297/1
type: Range
persistent identity: range1990297
display id: range1990297
version: 1
direction: direction/forward
top level: SP-Lipocal
start: 4
end: 9
orientation: inline

Scar (Thr, Gly)
type: SequenceAnnotation
persistent identity: annotation1993412
display id: annotation1993412
version: 1
top level: SP-Lipocal
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157037/annotation1993412/range1993412/1

https://synbiohub.org/public/igem/BBa_K157037/annotation1993412/range1993412/1
type: Range
persistent identity: range1993412
display id: range1993412
version: 1
direction: direction/forward
top level: SP-Lipocal
start: 610
end: 615
orientation: inline

SP
type: SequenceAnnotation
persistent identity: annotation1993409
display id: annotation1993409
version: 1
top level: SP-Lipocal
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157037/annotation1993409/range1993409/1

https://synbiohub.org/public/igem/BBa_K157037/annotation1993409/range1993409/1
type: Range
persistent identity: range1993409
display id: range1993409
version: 1
direction: direction/forward
top level: SP-Lipocal
start: 10
end: 81
orientation: inline

Lipocalin
type: SequenceAnnotation
persistent identity: annotation1993411
display id: annotation1993411
version: 1
top level: SP-Lipocal
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157037/annotation1993411/range1993411/1

https://synbiohub.org/public/igem/BBa_K157037/annotation1993411/range1993411/1
type: Range
persistent identity: range1993411
display id: range1993411
version: 1
direction: direction/forward
top level: SP-Lipocal
start: 88
end: 609
orientation: inline

Scar (Thr, Gly)
type: SequenceAnnotation
persistent identity: annotation1993414
display id: annotation1993414
version: 1
top level: SP-Lipocal
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157037/annotation1993414/range1993414/1

https://synbiohub.org/public/igem/BBa_K157037/annotation1993414/range1993414/1
type: Range
persistent identity: range1993414
display id: range1993414
version: 1
direction: direction/forward
top level: SP-Lipocal
start: 661
end: 666
orientation: inline

SP-Lipocalin-GGGSlinker-Tm-nCFP
type: SequenceAnnotation
persistent identity: annotation1990299
display id: annotation1990299
version: 1
top level: SP-Lipocal
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K157037/annotation1990299/range1990299/1

https://synbiohub.org/public/igem/BBa_K157037/annotation1990299/range1990299/1
type: Range
persistent identity: range1990299
display id: range1990299
version: 1
direction: direction/forward
top level: SP-Lipocal
start: 1
end: 1212
orientation: inline

AgeI
type: SequenceAnnotation
persistent identity: annotation1990298
display id: annotation1990298
version: 1
top level: SP-Lipocal
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157037/annotation1990298/range1990298/1

https://synbiohub.org/public/igem/BBa_K157037/annotation1990298/range1990298/1
type: Range
persistent identity: range1990298
display id: range1990298
version: 1
direction: direction/forward
top level: SP-Lipocal
start: 1207
end: 1212
orientation: inline

Tm
type: SequenceAnnotation
persistent identity: annotation1993415
display id: annotation1993415
version: 1
top level: SP-Lipocal
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157037/annotation1993415/range1993415/1

https://synbiohub.org/public/igem/BBa_K157037/annotation1993415/range1993415/1
type: Range
persistent identity: range1993415
display id: range1993415
version: 1
direction: direction/forward
top level: SP-Lipocal
start: 667
end: 735
orientation: inline

Scar (Thr, Gly)
type: SequenceAnnotation
persistent identity: annotation1993416
display id: annotation1993416
version: 1
top level: SP-Lipocal
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157037/annotation1993416/range1993416/1

https://synbiohub.org/public/igem/BBa_K157037/annotation1993416/range1993416/1
type: Range
persistent identity: range1993416
display id: range1993416
version: 1
direction: direction/forward
top level: SP-Lipocal
start: 736
end: 741
orientation: inline

N-Term_CFP
type: SequenceAnnotation
persistent identity: annotation1993417
display id: annotation1993417
version: 1
top level: SP-Lipocal
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157037/annotation1993417/range1993417/1

https://synbiohub.org/public/igem/BBa_K157037/annotation1993417/range1993417/1
type: Range
persistent identity: range1993417
display id: range1993417
version: 1
direction: direction/forward
top level: SP-Lipocal
start: 742
end: 1206
orientation: inline

Scar (Thr, Gly)
type: SequenceAnnotation
persistent identity: annotation1993410
display id: annotation1993410
version: 1
top level: SP-Lipocal
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157037/annotation1993410/range1993410/1

https://synbiohub.org/public/igem/BBa_K157037/annotation1993410/range1993410/1
type: Range
persistent identity: range1993410
display id: range1993410
version: 1
direction: direction/forward
top level: SP-Lipocal
start: 82
end: 87
orientation: inline

BBa_K879311
type: ComponentDefinition
persistent identity: BBa K879311
display id: BBa_K879311
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Red with promoter 3
creator: Nien-Tsu Tseng
created: 2012-09-30T11:00:00Z
modified: 2015-05-08T01:13:39Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1141_
m user id: 0
owner id: 13537
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: Red with promoter 3
mutable notes: .
mutable provenance: .
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K879091
component: BBa_K879033
component: BBa_K879021
component: BBa_K879092
component: BBa_K879011
sequence annotation: BBa_K879021
sequence annotation: BBa_K879091
sequence annotation: BBa_K879011
sequence annotation: BBa_K879033
sequence annotation: BBa_K879092
sequence: https://synbiohub.org/public/igem/BBa_K879311_sequence/1

BBa_K879091
type: Component
persistent identity: component2205030
display id: component2205030
version: 1
top level: BBa_K879311
access: public
definition: https://synbiohub.org/public/igem/BBa_K879091/1

BBa_K879033
type: Component
persistent identity: component2205031
display id: component2205031
version: 1
top level: BBa_K879311
access: public
definition: https://synbiohub.org/public/igem/BBa_K879033/1

BBa_K879021
type: Component
persistent identity: component2205033
display id: component2205033
version: 1
top level: BBa_K879311
access: public
definition: https://synbiohub.org/public/igem/BBa_K879021/1

BBa_K879092
type: Component
persistent identity: component2205032
display id: component2205032
version: 1
top level: BBa_K879311
access: public
definition: https://synbiohub.org/public/igem/BBa_K879092/1

BBa_K879011
type: Component
persistent identity: component2205034
display id: component2205034
version: 1
top level: BBa_K879311
access: public
definition: https://synbiohub.org/public/igem/BBa_K879011/1

BBa_K879021
type: SequenceAnnotation
persistent identity: annotation2205033
display id: annotation2205033
version: 1
top level: BBa_K879311
location: https://synbiohub.org/public/igem/BBa_K879311/annotation2205033/range2205033/1
component: BBa_K879021

https://synbiohub.org/public/igem/BBa_K879311/annotation2205033/range2205033/1
type: Range
persistent identity: range2205033
display id: range2205033
version: 1
direction: direction/reverse
top level: BBa_K879311
start: 75
end: 100
orientation: inline

BBa_K879091
type: SequenceAnnotation
persistent identity: annotation2205030
display id: annotation2205030
version: 1
top level: BBa_K879311
location: https://synbiohub.org/public/igem/BBa_K879311/annotation2205030/range2205030/1
component: BBa_K879091

https://synbiohub.org/public/igem/BBa_K879311/annotation2205030/range2205030/1
type: Range
persistent identity: range2205030
display id: range2205030
version: 1
direction: direction/reverse
top level: BBa_K879311
start: 1
end: 36
orientation: inline

BBa_K879011
type: SequenceAnnotation
persistent identity: annotation2205034
display id: annotation2205034
version: 1
top level: BBa_K879311
location: https://synbiohub.org/public/igem/BBa_K879311/annotation2205034/range2205034/1
component: BBa_K879011

https://synbiohub.org/public/igem/BBa_K879311/annotation2205034/range2205034/1
type: Range
persistent identity: range2205034
display id: range2205034
version: 1
direction: direction/reverse
top level: BBa_K879311
start: 101
end: 781
orientation: inline

BBa_K879033
type: SequenceAnnotation
persistent identity: annotation2205031
display id: annotation2205031
version: 1
top level: BBa_K879311
location: https://synbiohub.org/public/igem/BBa_K879311/annotation2205031/range2205031/1
component: BBa_K879033

https://synbiohub.org/public/igem/BBa_K879311/annotation2205031/range2205031/1
type: Range
persistent identity: range2205031
display id: range2205031
version: 1
direction: direction/reverse
top level: BBa_K879311
start: 37
end: 65
orientation: inline

BBa_K879092
type: SequenceAnnotation
persistent identity: annotation2205032
display id: annotation2205032
version: 1
top level: BBa_K879311
location: https://synbiohub.org/public/igem/BBa_K879311/annotation2205032/range2205032/1
component: BBa_K879092

https://synbiohub.org/public/igem/BBa_K879311/annotation2205032/range2205032/1
type: Range
persistent identity: range2205032
display id: range2205032
version: 1
direction: direction/reverse
top level: BBa_K879311
start: 66
end: 74
orientation: inline

PlsirE5501
type: ComponentDefinition
persistent identity: BBa K1725402
display id: BBa_K1725402
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PlsiR + B0032 + GFP
creator: Andrey Filipov
created: 2015-09-07T11:00:00Z
modified: 2015-09-08T11:23:05Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2146_
m user id: 21308
owner id: 21308
owning group id: 9
status: status/Planning
bookmark: false
mutable description: The promoter activated by the release of UirR (K1725420) from the transmembrane protein UirS (K1725410) in response to unidirectional UV-A light is upstream of GFP with the B0032 RBS (BBa_E5501). This allows activity in response to UV-A to be measured. This construct is expected to result in less fluorescence than K1725401. For the promoter to be activated UirR and UirS must be present and the cell has to be exposed to UV-A.
mutable notes: The promoter, including the BioBrick prefix and suffix, was ordered as a G-block. It was ligated to E5501 after restriction that was followed by an Azure-A-based gel extraction.
mutable provenance: The promoter comes from the genome of the unicellular cyanobacterium Synechocystis sp. PCC6803.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_E5501
component: BBa_K1725400
sequence annotation: BBa_K1725400
sequence annotation: BBa_E5501
sequence: https://synbiohub.org/public/igem/BBa_K1725402_sequence/1

BBa_E5501
type: Component
persistent identity: component2447009
display id: component2447009
version: 1
top level: PlsirE5501
access: public
definition: https://synbiohub.org/public/igem/BBa_E5501/1

BBa_K1725400
type: Component
persistent identity: component2447004
display id: component2447004
version: 1
top level: PlsirE5501
access: public
definition: https://synbiohub.org/public/igem/BBa_K1725400/1

BBa_K1725400
type: SequenceAnnotation
persistent identity: annotation2447004
display id: annotation2447004
version: 1
top level: PlsirE5501
location: https://synbiohub.org/public/igem/BBa_K1725402/annotation2447004/range2447004/1
component: BBa_K1725400

https://synbiohub.org/public/igem/BBa_K1725402/annotation2447004/range2447004/1
type: Range
persistent identity: range2447004
display id: range2447004
version: 1
direction: direction/reverse
top level: PlsirE5501
start: 1
end: 534
orientation: inline

BBa_E5501
type: SequenceAnnotation
persistent identity: annotation2447009
display id: annotation2447009
version: 1
top level: PlsirE5501
location: https://synbiohub.org/public/igem/BBa_K1725402/annotation2447009/range2447009/1
component: BBa_E5501

https://synbiohub.org/public/igem/BBa_K1725402/annotation2447009/range2447009/1
type: Range
persistent identity: range2447009
display id: range2447009
version: 1
direction: direction/reverse
top level: PlsirE5501
start: 543
end: 1281
orientation: inline

BBa_K1819007
type: ComponentDefinition
persistent identity: BBa K1819007
display id: BBa_K1819007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Constitutive promoter (J23117) and GFP (S05308)
creator: La??s Ribovski
created: 2015-09-15T11:00:00Z
modified: 2015-09-16T05:45:22Z
discontinued: false
dominant: false
group u list: _2244_
m user id: 21190
owner id: 21190
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Constitutive promotor from Anderson library (BBa_J23117) assembled to green fluorescent protein attached to linker (BBa_S05308). This part was used to characterize BBa_K1819000 and compare its efficiency to green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (BBa_E0040).
mutable notes: No considerations
mutable provenance: This part results from the assembly of BBa_J23117 and BBa_S05308
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_S05308
component: BBa_J23117
sequence annotation: BBa_S05308
sequence annotation: BBa_J23117
sequence: https://synbiohub.org/public/igem/BBa_K1819007_sequence/1

BBa_S05308
type: Component
persistent identity: component2462531
display id: component2462531
version: 1
top level: BBa_K1819007
access: public
definition: https://synbiohub.org/public/igem/BBa_S05308/1

BBa_J23117
type: Component
persistent identity: component2462520
display id: component2462520
version: 1
top level: BBa_K1819007
access: public
definition: https://synbiohub.org/public/igem/BBa_J23117/1

BBa_S05308
type: SequenceAnnotation
persistent identity: annotation2462531
display id: annotation2462531
version: 1
top level: BBa_K1819007
location: https://synbiohub.org/public/igem/BBa_K1819007/annotation2462531/range2462531/1
component: BBa_S05308

https://synbiohub.org/public/igem/BBa_K1819007/annotation2462531/range2462531/1
type: Range
persistent identity: range2462531
display id: range2462531
version: 1
direction: direction/reverse
top level: BBa_K1819007
start: 44
end: 930
orientation: inline

BBa_J23117
type: SequenceAnnotation
persistent identity: annotation2462520
display id: annotation2462520
version: 1
top level: BBa_K1819007
location: https://synbiohub.org/public/igem/BBa_K1819007/annotation2462520/range2462520/1
component: BBa_J23117

https://synbiohub.org/public/igem/BBa_K1819007/annotation2462520/range2462520/1
type: Range
persistent identity: range2462520
display id: range2462520
version: 1
direction: direction/reverse
top level: BBa_K1819007
start: 1
end: 35
orientation: inline

BBa_K772008
type: ComponentDefinition
persistent identity: BBa K772008
display id: BBa_K772008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: GFP - LacZ protein complex with flag tags
creator: Mustafa Elitok
created: 2012-09-25T11:00:00Z
modified: 2015-05-08T01:13:15Z
discontinued: false
dominant: false
group u list: _1024_
m user id: 0
owner id: 9573
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This part contains a green fluorescent protein (GFP) as a non-reactive chemical compound (anchor) in order to neutralize the active reactive fixed to it, a reporter device (LacZ enzyme catalyses the reaction of the cleavage of X-Gal which results with blue color change) and a TEV protease cleavage site between them to assemble these two proteins. BBa_772001 and BBa_K772002 are assembled to be used as the trigger of detection in the whole system which ends with the intracellular transfection (or activation) of TEV protease. After the activation, it is planned that TEV protease will cleave this anchor-inducer protein complex to release free the reporter. In conclusion, in this system, the inducer would activate a transcription pathway on the targeted inducible gene or the reporter would indicate that there is a change in the whole system. This part contains flag tags extra from BBa_K772006 in the GFP region; because it is planned to be used in the Co-IP/SDS-PAGE assay to be isolated.
mutable notes: Cleavage site, LacZ and GFP sequences are gathered from Registry.
mutable provenance: Note that there is an RBS sequence just before the protein sequence in order to eliminate the need of addition of it.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
sequence annotation: LacZ
sequence annotation: Double Terminator
sequence annotation: Anchor-Reporter + Flag
sequence annotation: TEV cleavage site
sequence annotation: GFP
sequence annotation: RBS
sequence annotation: Flag Tag
sequence: https://synbiohub.org/public/igem/BBa_K772008_sequence/1

LacZ
type: SequenceAnnotation
persistent identity: annotation2203914
display id: annotation2203914
version: 1
top level: BBa_K772008
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K772008/annotation2203914/range2203914/1

https://synbiohub.org/public/igem/BBa_K772008/annotation2203914/range2203914/1
type: Range
persistent identity: range2203914
display id: range2203914
version: 1
direction: direction/forward
top level: BBa_K772008
start: 858
end: 1101
orientation: inline

Double Terminator
type: SequenceAnnotation
persistent identity: annotation2203915
display id: annotation2203915
version: 1
top level: BBa_K772008
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K772008/annotation2203915/range2203915/1

https://synbiohub.org/public/igem/BBa_K772008/annotation2203915/range2203915/1
type: Range
persistent identity: range2203915
display id: range2203915
version: 1
direction: direction/forward
top level: BBa_K772008
start: 1102
end: 1232
orientation: inline

Anchor-Reporter + Flag
type: SequenceAnnotation
persistent identity: annotation2203916
display id: annotation2203916
version: 1
top level: BBa_K772008
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K772008/annotation2203916/range2203916/1

https://synbiohub.org/public/igem/BBa_K772008/annotation2203916/range2203916/1
type: Range
persistent identity: range2203916
display id: range2203916
version: 1
direction: direction/forward
top level: BBa_K772008
start: 21
end: 1101
orientation: inline

TEV cleavage site
type: SequenceAnnotation
persistent identity: annotation2203913
display id: annotation2203913
version: 1
top level: BBa_K772008
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K772008/annotation2203913/range2203913/1

https://synbiohub.org/public/igem/BBa_K772008/annotation2203913/range2203913/1
type: Range
persistent identity: range2203913
display id: range2203913
version: 1
direction: direction/forward
top level: BBa_K772008
start: 768
end: 857
orientation: inline

GFP
type: SequenceAnnotation
persistent identity: annotation2203912
display id: annotation2203912
version: 1
top level: BBa_K772008
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K772008/annotation2203912/range2203912/1

https://synbiohub.org/public/igem/BBa_K772008/annotation2203912/range2203912/1
type: Range
persistent identity: range2203912
display id: range2203912
version: 1
direction: direction/forward
top level: BBa_K772008
start: 47
end: 767
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2203910
display id: annotation2203910
version: 1
top level: BBa_K772008
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K772008/annotation2203910/range2203910/1

https://synbiohub.org/public/igem/BBa_K772008/annotation2203910/range2203910/1
type: Range
persistent identity: range2203910
display id: range2203910
version: 1
direction: direction/forward
top level: BBa_K772008
start: 1
end: 20
orientation: inline

Flag Tag
type: SequenceAnnotation
persistent identity: annotation2203911
display id: annotation2203911
version: 1
top level: BBa_K772008
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K772008/annotation2203911/range2203911/1

https://synbiohub.org/public/igem/BBa_K772008/annotation2203911/range2203911/1
type: Range
persistent identity: range2203911
display id: range2203911
version: 1
direction: direction/forward
top level: BBa_K772008
start: 21
end: 46
orientation: inline

BBa_K283025
type: ComponentDefinition
persistent identity: BBa K283025
display id: BBa_K283025
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: prosseA-rbs-RFP-terminator
creator: W Ling , Shi Lei, Nelson Chu
created: 2009-10-12T11:00:00Z
modified: 2015-05-08T01:11:47Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _383_
m user id: 0
owner id: 5494
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part is a generator for RFP under the prosseA promoter
mutable notes: BBa_K283025
mutable provenance: The prosseA promoter is self-designed The rbs, RFP and the terminator are from the registry
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_K283038
component: BBa_B0012
component: BBa_B0034
component: BBa_E1010
component: BBa_B0010
sequence annotation: BBa_B0034
sequence annotation: BBa_B0012
sequence annotation: BBa_B0010
sequence annotation: BBa_E1010
sequence annotation: BBa_K283038
sequence: https://synbiohub.org/public/igem/BBa_K283025_sequence/1

BBa_K283038
type: Component
persistent identity: component2256019
display id: component2256019
version: 1
top level: BBa_K283025
access: public
definition: https://synbiohub.org/public/igem/BBa_K283038/1

BBa_B0012
type: Component
persistent identity: component2256027
display id: component2256027
version: 1
top level: BBa_K283025
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0034
type: Component
persistent identity: component2256021
display id: component2256021
version: 1
top level: BBa_K283025
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_E1010
type: Component
persistent identity: component2256024
display id: component2256024
version: 1
top level: BBa_K283025
access: public
definition: mRFP1

BBa_B0010
type: Component
persistent identity: component2256025
display id: component2256025
version: 1
top level: BBa_K283025
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2256021
display id: annotation2256021
version: 1
top level: BBa_K283025
location: https://synbiohub.org/public/igem/BBa_K283025/annotation2256021/range2256021/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K283025/annotation2256021/range2256021/1
type: Range
persistent identity: range2256021
display id: range2256021
version: 1
direction: direction/reverse
top level: BBa_K283025
start: 509
end: 520
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2256027
display id: annotation2256027
version: 1
top level: BBa_K283025
location: https://synbiohub.org/public/igem/BBa_K283025/annotation2256027/range2256027/1
component: BBa_B0012

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type: Range
persistent identity: range2256027
display id: range2256027
version: 1
direction: direction/forward
top level: BBa_K283025
start: 1329
end: 1369
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2256025
display id: annotation2256025
version: 1
top level: BBa_K283025
location: https://synbiohub.org/public/igem/BBa_K283025/annotation2256025/range2256025/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K283025/annotation2256025/range2256025/1
type: Range
persistent identity: range2256025
display id: range2256025
version: 1
direction: direction/forward
top level: BBa_K283025
start: 1241
end: 1320
orientation: inline

BBa_E1010
type: SequenceAnnotation
persistent identity: annotation2256024
display id: annotation2256024
version: 1
top level: BBa_K283025
location: https://synbiohub.org/public/igem/BBa_K283025/annotation2256024/range2256024/1
component: BBa_E1010

https://synbiohub.org/public/igem/BBa_K283025/annotation2256024/range2256024/1
type: Range
persistent identity: range2256024
display id: range2256024
version: 1
direction: direction/reverse
top level: BBa_K283025
start: 527
end: 1232
orientation: inline

BBa_K283038
type: SequenceAnnotation
persistent identity: annotation2256019
display id: annotation2256019
version: 1
top level: BBa_K283025
location: https://synbiohub.org/public/igem/BBa_K283025/annotation2256019/range2256019/1
component: BBa_K283038

https://synbiohub.org/public/igem/BBa_K283025/annotation2256019/range2256019/1
type: Range
persistent identity: range2256019
display id: range2256019
version: 1
direction: direction/reverse
top level: BBa_K283025
start: 1
end: 500
orientation: inline

BBa_K1486007
type: ComponentDefinition
persistent identity: BBa K1486007
display id: BBa_K1486007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: IFP[2]
creator: iGem EPFL 2014
created: 2014-08-24T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is the second part of the split infrared fluorescent protein (IFP1.4). To be used with IFP[1].
mutable notes: When fused to another coding sequence, insert a flexible linker.
mutable provenance: From Michnick Article. To Edit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: START
sequence annotation: IFP[2]
sequence annotation: STOP
sequence: https://synbiohub.org/public/igem/BBa_K1486007_sequence/1

START
type: SequenceAnnotation
persistent identity: annotation2382128
display id: annotation2382128
version: 1
top level: BBa_K1486007
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1486007/annotation2382128/range2382128/1

https://synbiohub.org/public/igem/BBa_K1486007/annotation2382128/range2382128/1
type: Range
persistent identity: range2382128
display id: range2382128
version: 1
direction: direction/forward
top level: BBa_K1486007
start: 1
end: 3
orientation: inline

IFP[2]
type: SequenceAnnotation
persistent identity: annotation2382164
display id: annotation2382164
version: 1
top level: BBa_K1486007
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1486007/annotation2382164/range2382164/1

https://synbiohub.org/public/igem/BBa_K1486007/annotation2382164/range2382164/1
type: Range
persistent identity: range2382164
display id: range2382164
version: 1
direction: direction/forward
top level: BBa_K1486007
start: 1
end: 579
orientation: inline

STOP
type: SequenceAnnotation
persistent identity: annotation2382163
display id: annotation2382163
version: 1
top level: BBa_K1486007
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1486007/annotation2382163/range2382163/1

https://synbiohub.org/public/igem/BBa_K1486007/annotation2382163/range2382163/1
type: Range
persistent identity: range2382163
display id: range2382163
version: 1
direction: direction/forward
top level: BBa_K1486007
start: 577
end: 579
orientation: inline

BBa_K206002
type: ComponentDefinition
persistent identity: BBa K206002
display id: BBa_K206002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RFP generator for testing pBAD strong
creator: Amelia Hardjasa
created: 2009-10-17T11:00:00Z
modified: 2015-05-08T01:11:23Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _307_
m user id: 0
owner id: 4172
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: We used this construct to test pBAD strong promoter strength by measuring RFP fluorescence.
mutable notes: No special design considerations.
mutable provenance: Assembled from Registry BioBricks.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K206000
component: BBa_I13507
sequence annotation: BBa_K206000
sequence annotation: BBa_I13507
sequence: https://synbiohub.org/public/igem/BBa_K206002_sequence/1

BBa_K206000
type: Component
persistent identity: component2256261
display id: component2256261
version: 1
top level: BBa_K206002
access: public
definition: https://synbiohub.org/public/igem/BBa_K206000/1

BBa_I13507
type: Component
persistent identity: component2256273
display id: component2256273
version: 1
top level: BBa_K206002
access: public
definition: https://synbiohub.org/public/igem/BBa_I13507/1

BBa_K206000
type: SequenceAnnotation
persistent identity: annotation2256261
display id: annotation2256261
version: 1
top level: BBa_K206002
location: https://synbiohub.org/public/igem/BBa_K206002/annotation2256261/range2256261/1
component: BBa_K206000

https://synbiohub.org/public/igem/BBa_K206002/annotation2256261/range2256261/1
type: Range
persistent identity: range2256261
display id: range2256261
version: 1
direction: direction/reverse
top level: BBa_K206002
start: 1
end: 130
orientation: inline

BBa_I13507
type: SequenceAnnotation
persistent identity: annotation2256273
display id: annotation2256273
version: 1
top level: BBa_K206002
location: https://synbiohub.org/public/igem/BBa_K206002/annotation2256273/range2256273/1
component: BBa_I13507

https://synbiohub.org/public/igem/BBa_K206002/annotation2256273/range2256273/1
type: Range
persistent identity: range2256273
display id: range2256273
version: 1
direction: direction/reverse
top level: BBa_K206002
start: 139
end: 999
orientation: inline

yfp
type: ComponentDefinition
persistent identity: BBa K165005
display id: BBa_K165005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Venus YFP, yeast optimized for fusion
creator: John Szymanski
created: 2008-10-25T11:00:00Z
modified: 2015-05-08T01:10:55Z
discontinued: false
dominant: false
experience: experience/None
group u list: _267_
m user id: 0
owner id: 2512
owning group id: 58
sample status: In stock
status: status/Available
bookmark: true
mutable description: This is the venus YFP, yeast optimized in the Silver Lab BioFusion standard for fusion of protein domains.
mutable notes: -
mutable provenance: From Caroline Ajo-Franklin
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K165005_sequence/1

BBa_K993002
type: ComponentDefinition
persistent identity: BBa K993002
display id: BBa_K993002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: IbpB RNA Thermometer with RFP
creator: İBRAHİM YASİR ORHAN
created: 2013-06-13T11:00:00Z
modified: 2015-05-08T01:13:51Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1290_
m user id: 0
owner id: 11153
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: The IbpB thermometer is an RNA thermometer element found in the ibpAB operon. The operon contains two heat-shock genes, encoding inclusion body binding proteins A and B (IbpA/B), and is the most drastically upregulated operon under heat-shock in Escherichia coli. IbpA is regulated by a ROSE element found in its 5' UTR, while IbpB has its own heat-sensitive cis-regulatory element. The activity of this thermoregulator was confirmed in vitro but was not found in vivo, suggesting more complicated operon regulation exists in bacterial cells. In this part, red fluorescent protein (RFP) is added in the downstream region to measure the activity of RNA thermometer. You check our Experience page for further information about our characterization and experiments. For the inner elements in the part design; check out the Part Design page. RNA Thermometers are the only known single-component regulators of gene expression.(1) RNA Thermometers are thermosensors that regulate gene expression by temperature-induced changes in RNA conformation. Naturally occurring RNA Thermometers exhibit complex secondary structures which are believed to undergo a series of gradual structural changes in response to temperature shifts.(2) RNA Thermometers often regulate genes required during either a heat shock or cold shock response, but have been implicated in other regulatory roles such as in pathogenecity and starvation.(3)
mutable notes: Note that RNA thermometer actually works as an RBS sequence. Therefore, this part does not include an additional ribosome binding site. Also, this part does not contain an terminator sequence, but a simple stop codon. The RFP should be translated continuously.
mutable provenance: Escherichia coli, NCBI GeneBank Other elements are obtained from Registry.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
sequence annotation: Stop codon
sequence annotation: pVeg2
sequence annotation: IbpB thermometer
sequence annotation: Start codon
sequence annotation: RFP
sequence: https://synbiohub.org/public/igem/BBa_K993002_sequence/1

Stop codon
type: SequenceAnnotation
persistent identity: annotation2329445
display id: annotation2329445
version: 1
top level: BBa_K993002
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K993002/annotation2329445/range2329445/1

https://synbiohub.org/public/igem/BBa_K993002/annotation2329445/range2329445/1
type: Range
persistent identity: range2329445
display id: range2329445
version: 1
direction: direction/forward
top level: BBa_K993002
start: 915
end: 917
orientation: inline

pVeg2
type: SequenceAnnotation
persistent identity: annotation2329442
display id: annotation2329442
version: 1
top level: BBa_K993002
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K993002/annotation2329442/range2329442/1

https://synbiohub.org/public/igem/BBa_K993002/annotation2329442/range2329442/1
type: Range
persistent identity: range2329442
display id: range2329442
version: 1
direction: direction/forward
top level: BBa_K993002
start: 1
end: 97
orientation: inline

IbpB thermometer
type: SequenceAnnotation
persistent identity: annotation2329443
display id: annotation2329443
version: 1
top level: BBa_K993002
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K993002/annotation2329443/range2329443/1

https://synbiohub.org/public/igem/BBa_K993002/annotation2329443/range2329443/1
type: Range
persistent identity: range2329443
display id: range2329443
version: 1
direction: direction/forward
top level: BBa_K993002
start: 98
end: 208
orientation: inline

Start codon
type: SequenceAnnotation
persistent identity: annotation2329446
display id: annotation2329446
version: 1
top level: BBa_K993002
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K993002/annotation2329446/range2329446/1

https://synbiohub.org/public/igem/BBa_K993002/annotation2329446/range2329446/1
type: Range
persistent identity: range2329446
display id: range2329446
version: 1
direction: direction/forward
top level: BBa_K993002
start: 209
end: 211
orientation: inline

RFP
type: SequenceAnnotation
persistent identity: annotation2329444
display id: annotation2329444
version: 1
top level: BBa_K993002
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K993002/annotation2329444/range2329444/1

https://synbiohub.org/public/igem/BBa_K993002/annotation2329444/range2329444/1
type: Range
persistent identity: range2329444
display id: range2329444
version: 1
direction: direction/forward
top level: BBa_K993002
start: 209
end: 917
orientation: inline

BBa_K1321357
type: ComponentDefinition
persistent identity: BBa K1321357
display id: BBa_K1321357
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: sfGFP fused to CBDcex driven by LacI
creator: Chris N Micklem
created: 2014-10-07T11:00:00Z
modified: 2016-01-26T09:45:49Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1696_
m user id: 4206
owner id: 20830
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: asdf
mutable notes: asdf
mutable provenance: asdf
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_J04500
component: BBa_K1319106
component: BBa_K1321342
sequence annotation: BBa_K1321342
sequence annotation: BBa_K1319106
sequence annotation: BBa_J04500
sequence: https://synbiohub.org/public/igem/BBa_K1321357_sequence/1

BBa_J04500
type: Component
persistent identity: component2415499
display id: component2415499
version: 1
top level: BBa_K1321357
access: public
definition: https://synbiohub.org/public/igem/BBa_J04500/1

BBa_K1319106
type: Component
persistent identity: component2415501
display id: component2415501
version: 1
top level: BBa_K1321357
access: public
definition: https://synbiohub.org/public/igem/BBa_K1319106/1

BBa_K1321342
type: Component
persistent identity: component2415507
display id: component2415507
version: 1
top level: BBa_K1321357
access: public
definition: https://synbiohub.org/public/igem/BBa_K1321342/1

BBa_K1321342
type: SequenceAnnotation
persistent identity: annotation2415507
display id: annotation2415507
version: 1
top level: BBa_K1321357
location: https://synbiohub.org/public/igem/BBa_K1321357/annotation2415507/range2415507/1
component: BBa_K1321342

https://synbiohub.org/public/igem/BBa_K1321357/annotation2415507/range2415507/1
type: Range
persistent identity: range2415507
display id: range2415507
version: 1
direction: direction/reverse
top level: BBa_K1321357
start: 236
end: 1279
orientation: inline

BBa_K1319106
type: SequenceAnnotation
persistent identity: annotation2415501
display id: annotation2415501
version: 1
top level: BBa_K1321357
location: https://synbiohub.org/public/igem/BBa_K1321357/annotation2415501/range2415501/1
component: BBa_K1319106

https://synbiohub.org/public/igem/BBa_K1321357/annotation2415501/range2415501/1
type: Range
persistent identity: range2415501
display id: range2415501
version: 1
direction: direction/reverse
top level: BBa_K1321357
start: 221
end: 235
orientation: inline

BBa_J04500
type: SequenceAnnotation
persistent identity: annotation2415499
display id: annotation2415499
version: 1
top level: BBa_K1321357
location: https://synbiohub.org/public/igem/BBa_K1321357/annotation2415499/range2415499/1
component: BBa_J04500

https://synbiohub.org/public/igem/BBa_K1321357/annotation2415499/range2415499/1
type: Range
persistent identity: range2415499
display id: range2415499
version: 1
direction: direction/reverse
top level: BBa_K1321357
start: 1
end: 220
orientation: inline

BBa_K206007
type: ComponentDefinition
persistent identity: BBa K206007
display id: BBa_K206007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: GFP generator for testing pBAD wt
creator: Amelia Hardjasa
created: 2009-10-18T11:00:00Z
modified: 2015-05-08T01:11:24Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _307_
m user id: 0
owner id: 4172
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This is a construct for testing promoter strength by measuring GFP fluorescence.
mutable notes: No special design considerations.
mutable provenance: Assembled from Registry BioBricks.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_I13453
component: BBa_B0010
component: BBa_B0012
component: BBa_J04031
component: BBa_B0034
sequence annotation: BBa_B0012
sequence annotation: BBa_J04031
sequence annotation: BBa_B0010
sequence annotation: BBa_I13453
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K206007_sequence/1

BBa_I13453
type: Component
persistent identity: component2054366
display id: component2054366
version: 1
top level: BBa_K206007
access: public
definition: https://synbiohub.org/public/igem/BBa_I13453/1

BBa_B0010
type: Component
persistent identity: component2054374
display id: component2054374
version: 1
top level: BBa_K206007
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
type: Component
persistent identity: component2054376
display id: component2054376
version: 1
top level: BBa_K206007
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_J04031
type: Component
persistent identity: component2054373
display id: component2054373
version: 1
top level: BBa_K206007
access: public
definition: https://synbiohub.org/public/igem/BBa_J04031/1

BBa_B0034
type: Component
persistent identity: component2054368
display id: component2054368
version: 1
top level: BBa_K206007
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2054376
display id: annotation2054376
version: 1
top level: BBa_K206007
location: https://synbiohub.org/public/igem/BBa_K206007/annotation2054376/range2054376/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K206007/annotation2054376/range2054376/1
type: Range
persistent identity: range2054376
display id: range2054376
version: 1
direction: direction/forward
top level: BBa_K206007
start: 1012
end: 1052
orientation: inline

BBa_J04031
type: SequenceAnnotation
persistent identity: annotation2054373
display id: annotation2054373
version: 1
top level: BBa_K206007
location: https://synbiohub.org/public/igem/BBa_K206007/annotation2054373/range2054373/1
component: BBa_J04031

https://synbiohub.org/public/igem/BBa_K206007/annotation2054373/range2054373/1
type: Range
persistent identity: range2054373
display id: range2054373
version: 1
direction: direction/reverse
top level: BBa_K206007
start: 157
end: 915
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2054374
display id: annotation2054374
version: 1
top level: BBa_K206007
location: https://synbiohub.org/public/igem/BBa_K206007/annotation2054374/range2054374/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K206007/annotation2054374/range2054374/1
type: Range
persistent identity: range2054374
display id: range2054374
version: 1
direction: direction/forward
top level: BBa_K206007
start: 924
end: 1003
orientation: inline

BBa_I13453
type: SequenceAnnotation
persistent identity: annotation2054366
display id: annotation2054366
version: 1
top level: BBa_K206007
location: https://synbiohub.org/public/igem/BBa_K206007/annotation2054366/range2054366/1
component: BBa_I13453

https://synbiohub.org/public/igem/BBa_K206007/annotation2054366/range2054366/1
type: Range
persistent identity: range2054366
display id: range2054366
version: 1
direction: direction/reverse
top level: BBa_K206007
start: 1
end: 130
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2054368
display id: annotation2054368
version: 1
top level: BBa_K206007
location: https://synbiohub.org/public/igem/BBa_K206007/annotation2054368/range2054368/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K206007/annotation2054368/range2054368/1
type: Range
persistent identity: range2054368
display id: range2054368
version: 1
direction: direction/reverse
top level: BBa_K206007
start: 139
end: 150
orientation: inline

BBa_K1679001
type: ComponentDefinition
persistent identity: BBa K1679001
display id: BBa_K1679001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: GFP Reporter
creator: Qikai Qin
created: 2015-08-19T11:00:00Z
modified: 2015-08-20T10:11:20Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2097_
m user id: 21726
owner id: 21726
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This is a device containing a promoter (BBa_J23101),a RBS (BBa_B0034), a GFP coding sequence (BBa_E0040) and two terminators (BBa_B0010, BBa_B0012) on the pSB1C3, which can be easily transformed into E.coli and produce GFP (mut3b). This is a GFP expression device, which can express GFP (mut3b) without any induction.
mutable notes: This is an InterLab Study required device in 2015, which was characterized by OUC-China.
mutable provenance: BBa_J23101 BBa_B0034 BBa_E0040 BBa_B0010 BBa_B0012
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_I13504
component: BBa_J23101
sequence annotation: BBa_I13504
sequence annotation: BBa_J23101
sequence: https://synbiohub.org/public/igem/BBa_K1679001_sequence/1

BBa_I13504
type: Component
persistent identity: component2436540
display id: component2436540
version: 1
top level: BBa_K1679001
access: public
definition: https://synbiohub.org/public/igem/BBa_I13504/1

BBa_J23101
type: Component
persistent identity: component2436530
display id: component2436530
version: 1
top level: BBa_K1679001
access: public
definition: https://synbiohub.org/public/igem/BBa_J23101/1

BBa_I13504
type: SequenceAnnotation
persistent identity: annotation2436540
display id: annotation2436540
version: 1
top level: BBa_K1679001
location: https://synbiohub.org/public/igem/BBa_K1679001/annotation2436540/range2436540/1
component: BBa_I13504

https://synbiohub.org/public/igem/BBa_K1679001/annotation2436540/range2436540/1
type: Range
persistent identity: range2436540
display id: range2436540
version: 1
direction: direction/reverse
top level: BBa_K1679001
start: 44
end: 918
orientation: inline

BBa_J23101
type: SequenceAnnotation
persistent identity: annotation2436530
display id: annotation2436530
version: 1
top level: BBa_K1679001
location: https://synbiohub.org/public/igem/BBa_K1679001/annotation2436530/range2436530/1
component: BBa_J23101

https://synbiohub.org/public/igem/BBa_K1679001/annotation2436530/range2436530/1
type: Range
persistent identity: range2436530
display id: range2436530
version: 1
direction: direction/reverse
top level: BBa_K1679001
start: 1
end: 35
orientation: inline

SbpA | RFP
type: ComponentDefinition
persistent identity: BBa K525404
display id: BBa_K525404
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Fusion Protein of S-Layer SbpA and mCherry RFP
creator: Timo Wolf
created: 2011-09-09T11:00:00Z
modified: 2016-01-26T09:41:39Z
discontinued: false
dominant: false
experience: experience/None
group u list: _690_
m user id: 4206
owner id: 6668
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: Fusion Protein of S-Layer sbpA and mCherry RFP
mutable notes: Fusion Protein of BBa_K525403 and BBa_J18932
mutable provenance: Fusion Protein of S-Layer sbpA and mCherry RFP
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: SbpA domain
sequence annotation: T7 promoter
sequence annotation: ATG
sequence annotation: AgeI
sequence annotation: TAA
sequence annotation: B0034
sequence annotation: mCherry (RFP)
sequence annotation: Freiburg scar
sequence annotation: K525401
sequence annotation: lac operator
sequence annotation: NgoMIV
sequence annotation: RBS
sequence annotation: J18932
sequence: https://synbiohub.org/public/igem/BBa_K525404_sequence/1

SbpA domain
type: SequenceAnnotation
persistent identity: annotation2129913
display id: annotation2129913
version: 1
top level: SbpA | RFP
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K525404/annotation2129913/range2129913/1

https://synbiohub.org/public/igem/BBa_K525404/annotation2129913/range2129913/1
type: Range
persistent identity: range2129913
display id: range2129913
version: 1
direction: direction/forward
top level: SbpA | RFP
start: 73
end: 3192
orientation: inline

T7 promoter
type: SequenceAnnotation
persistent identity: annotation2128864
display id: annotation2128864
version: 1
top level: SbpA | RFP
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K525404/annotation2128864/range2128864/1

https://synbiohub.org/public/igem/BBa_K525404/annotation2128864/range2128864/1
type: Range
persistent identity: range2128864
display id: range2128864
version: 1
direction: direction/forward
top level: SbpA | RFP
start: 1
end: 20
orientation: inline

ATG
type: SequenceAnnotation
persistent identity: annotation2129907
display id: annotation2129907
version: 1
top level: SbpA | RFP
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K525404/annotation2129907/range2129907/1

https://synbiohub.org/public/igem/BBa_K525404/annotation2129907/range2129907/1
type: Range
persistent identity: range2129907
display id: range2129907
version: 1
direction: direction/forward
top level: SbpA | RFP
start: 73
end: 75
orientation: inline

AgeI
type: SequenceAnnotation
persistent identity: annotation2129910
display id: annotation2129910
version: 1
top level: SbpA | RFP
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K525404/annotation2129910/range2129910/1

https://synbiohub.org/public/igem/BBa_K525404/annotation2129910/range2129910/1
type: Range
persistent identity: range2129910
display id: range2129910
version: 1
direction: direction/forward
top level: SbpA | RFP
start: 3904
end: 3909
orientation: inline

TAA
type: SequenceAnnotation
persistent identity: annotation2129911
display id: annotation2129911
version: 1
top level: SbpA | RFP
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K525404/annotation2129911/range2129911/1

https://synbiohub.org/public/igem/BBa_K525404/annotation2129911/range2129911/1
type: Range
persistent identity: range2129911
display id: range2129911
version: 1
direction: direction/forward
top level: SbpA | RFP
start: 3910
end: 3912
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation2129905
display id: annotation2129905
version: 1
top level: SbpA | RFP
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K525404/annotation2129905/range2129905/1

https://synbiohub.org/public/igem/BBa_K525404/annotation2129905/range2129905/1
type: Range
persistent identity: range2129905
display id: range2129905
version: 1
direction: direction/forward
top level: SbpA | RFP
start: 53
end: 64
orientation: inline

mCherry (RFP)
type: SequenceAnnotation
persistent identity: annotation2129915
display id: annotation2129915
version: 1
top level: SbpA | RFP
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K525404/annotation2129915/range2129915/1

https://synbiohub.org/public/igem/BBa_K525404/annotation2129915/range2129915/1
type: Range
persistent identity: range2129915
display id: range2129915
version: 1
direction: direction/forward
top level: SbpA | RFP
start: 3199
end: 3912
orientation: inline

Freiburg scar
type: SequenceAnnotation
persistent identity: annotation2129912
display id: annotation2129912
version: 1
top level: SbpA | RFP
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K525404/annotation2129912/range2129912/1

https://synbiohub.org/public/igem/BBa_K525404/annotation2129912/range2129912/1
type: Range
persistent identity: range2129912
display id: range2129912
version: 1
direction: direction/forward
top level: SbpA | RFP
start: 3193
end: 3198
orientation: inline

K525401
type: SequenceAnnotation
persistent identity: annotation2129908
display id: annotation2129908
version: 1
top level: SbpA | RFP
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K525404/annotation2129908/range2129908/1

https://synbiohub.org/public/igem/BBa_K525404/annotation2129908/range2129908/1
type: Range
persistent identity: range2129908
display id: range2129908
version: 1
direction: direction/forward
top level: SbpA | RFP
start: 73
end: 3192
orientation: inline

lac operator
type: SequenceAnnotation
persistent identity: annotation2128865
display id: annotation2128865
version: 1
top level: SbpA | RFP
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K525404/annotation2128865/range2128865/1

https://synbiohub.org/public/igem/BBa_K525404/annotation2128865/range2128865/1
type: Range
persistent identity: range2128865
display id: range2128865
version: 1
direction: direction/forward
top level: SbpA | RFP
start: 20
end: 44
orientation: inline

NgoMIV
type: SequenceAnnotation
persistent identity: annotation2129909
display id: annotation2129909
version: 1
top level: SbpA | RFP
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K525404/annotation2129909/range2129909/1

https://synbiohub.org/public/igem/BBa_K525404/annotation2129909/range2129909/1
type: Range
persistent identity: range2129909
display id: range2129909
version: 1
direction: direction/forward
top level: SbpA | RFP
start: 76
end: 81
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2129906
display id: annotation2129906
version: 1
top level: SbpA | RFP
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K525404/annotation2129906/range2129906/1

https://synbiohub.org/public/igem/BBa_K525404/annotation2129906/range2129906/1
type: Range
persistent identity: range2129906
display id: range2129906
version: 1
direction: direction/forward
top level: SbpA | RFP
start: 56
end: 59
orientation: inline

J18932
type: SequenceAnnotation
persistent identity: annotation2129914
display id: annotation2129914
version: 1
top level: SbpA | RFP
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K525404/annotation2129914/range2129914/1

https://synbiohub.org/public/igem/BBa_K525404/annotation2129914/range2129914/1
type: Range
persistent identity: range2129914
display id: range2129914
version: 1
direction: direction/forward
top level: SbpA | RFP
start: 3199
end: 3912
orientation: inline

BBa_K1486049
type: ComponentDefinition
persistent identity: BBa K1486049
display id: BBa_K1486049
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CpxR promoter FW + RFP
creator: EPFL iGem team 2014
created: 2014-10-06T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: to edit
mutable notes: to edit
mutable provenance: to edit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_I13507
component: BBa_K1486046
sequence annotation: BBa_I13507
sequence annotation: BBa_K1486046
sequence: https://synbiohub.org/public/igem/BBa_K1486049_sequence/1

BBa_I13507
type: Component
persistent identity: component2398436
display id: component2398436
version: 1
top level: BBa_K1486049
access: public
definition: https://synbiohub.org/public/igem/BBa_I13507/1

BBa_K1486046
type: Component
persistent identity: component2398424
display id: component2398424
version: 1
top level: BBa_K1486049
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486046/1

BBa_I13507
type: SequenceAnnotation
persistent identity: annotation2398436
display id: annotation2398436
version: 1
top level: BBa_K1486049
location: https://synbiohub.org/public/igem/BBa_K1486049/annotation2398436/range2398436/1
component: BBa_I13507

https://synbiohub.org/public/igem/BBa_K1486049/annotation2398436/range2398436/1
type: Range
persistent identity: range2398436
display id: range2398436
version: 1
direction: direction/reverse
top level: BBa_K1486049
start: 155
end: 1015
orientation: inline

BBa_K1486046
type: SequenceAnnotation
persistent identity: annotation2398424
display id: annotation2398424
version: 1
top level: BBa_K1486049
location: https://synbiohub.org/public/igem/BBa_K1486049/annotation2398424/range2398424/1
component: BBa_K1486046

https://synbiohub.org/public/igem/BBa_K1486049/annotation2398424/range2398424/1
type: Range
persistent identity: range2398424
display id: range2398424
version: 1
direction: direction/reverse
top level: BBa_K1486049
start: 1
end: 146
orientation: inline

BBa_K1486045
type: ComponentDefinition
persistent identity: BBa K1486045
display id: BBa_K1486045
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: IFP[2] Mutated
creator: EPFL iGem team 2014
created: 2014-10-06T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1866_
m user id: 0
owner id: 21033
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is a test
mutable notes: Test
mutable provenance: Test
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: IFP2
sequence: https://synbiohub.org/public/igem/BBa_K1486045_sequence/1

IFP2
type: SequenceAnnotation
persistent identity: annotation2420793
display id: annotation2420793
version: 1
top level: BBa_K1486045
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1486045/annotation2420793/range2420793/1

https://synbiohub.org/public/igem/BBa_K1486045/annotation2420793/range2420793/1
type: Range
persistent identity: range2420793
display id: range2420793
version: 1
direction: direction/forward
top level: BBa_K1486045
start: 1
end: 582
orientation: inline

GFP BD
type: ComponentDefinition
persistent identity: BBa K106000
display id: BBa_K106000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: GFP, AarI BD part
creator: Andrew Horwitz
created: 2008-10-13T11:00:00Z
modified: 2015-05-08T01:08:57Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _226_
m user id: 0
owner id: 2659
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This is the coding sequence of a yeast optimized GFP with BD ends for AarI cloning.
mutable notes: As a BD part, the stop codon is included.
mutable provenance: PCR amplified from lab plasmid stocks.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: GFP
sequence: https://synbiohub.org/public/igem/BBa_K106000_sequence/1

GFP
type: SequenceAnnotation
persistent identity: annotation1981456
display id: annotation1981456
version: 1
top level: GFP BD
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K106000/annotation1981456/range1981456/1

https://synbiohub.org/public/igem/BBa_K106000/annotation1981456/range1981456/1
type: Range
persistent identity: range1981456
display id: range1981456
version: 1
direction: direction/forward
top level: GFP BD
start: 1
end: 714
orientation: inline

BBa_K2023015
type: ComponentDefinition
persistent identity: BBa K2023015
display id: BBa_K2023015
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: XylR coding device-GFP coding device with Pu
creator: C??lia Chenebault
created: 2016-10-13T11:00:00Z
modified: 2016-10-20T03:40:10Z
discontinued: false
dominant: false
group u list: _2490_
m user id: 30356
owner id: 30230
owning group id: 9
status: status/Planning
bookmark: false
mutable description: XylR coding device-GFP coding device with Pu
mutable notes: blabla
mutable provenance: XylR coding device (BBa_K2023002) and GFP coding device with Pu (BBa_K2023016)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_K2023002
component: BBa_I723020
component: BBa_E0040
component: BBa_B0034
component: BBa_B0015
sequence annotation: BBa_K2023002
sequence annotation: BBa_E0040
sequence annotation: BBa_I723020
sequence annotation: BBa_B0015
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K2023015_sequence/1

BBa_K2023002
type: Component
persistent identity: component2518979
display id: component2518979
version: 1
top level: BBa_K2023015
access: public
definition: https://synbiohub.org/public/igem/BBa_K2023002/1

BBa_I723020
type: Component
persistent identity: component2518981
display id: component2518981
version: 1
top level: BBa_K2023015
access: public
definition: https://synbiohub.org/public/igem/BBa_I723020/1

BBa_E0040
type: Component
persistent identity: component2518985
display id: component2518985
version: 1
top level: BBa_K2023015
access: public
definition: GFP

BBa_B0034
type: Component
persistent identity: component2518983
display id: component2518983
version: 1
top level: BBa_K2023015
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0015
type: Component
persistent identity: component2518992
display id: component2518992
version: 1
top level: BBa_K2023015
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_K2023002
type: SequenceAnnotation
persistent identity: annotation2518979
display id: annotation2518979
version: 1
top level: BBa_K2023015
location: https://synbiohub.org/public/igem/BBa_K2023015/annotation2518979/range2518979/1
component: BBa_K2023002

https://synbiohub.org/public/igem/BBa_K2023015/annotation2518979/range2518979/1
type: Range
persistent identity: range2518979
display id: range2518979
version: 1
direction: direction/reverse
top level: BBa_K2023015
start: 1
end: 2243
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation2518985
display id: annotation2518985
version: 1
top level: BBa_K2023015
location: https://synbiohub.org/public/igem/BBa_K2023015/annotation2518985/range2518985/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_K2023015/annotation2518985/range2518985/1
type: Range
persistent identity: range2518985
display id: range2518985
version: 1
direction: direction/reverse
top level: BBa_K2023015
start: 2598
end: 3317
orientation: inline

BBa_I723020
type: SequenceAnnotation
persistent identity: annotation2518981
display id: annotation2518981
version: 1
top level: BBa_K2023015
location: https://synbiohub.org/public/igem/BBa_K2023015/annotation2518981/range2518981/1
component: BBa_I723020

https://synbiohub.org/public/igem/BBa_K2023015/annotation2518981/range2518981/1
type: Range
persistent identity: range2518981
display id: range2518981
version: 1
direction: direction/reverse
top level: BBa_K2023015
start: 2252
end: 2571
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2518992
display id: annotation2518992
version: 1
top level: BBa_K2023015
location: https://synbiohub.org/public/igem/BBa_K2023015/annotation2518992/range2518992/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K2023015/annotation2518992/range2518992/1
type: Range
persistent identity: range2518992
display id: range2518992
version: 1
direction: direction/reverse
top level: BBa_K2023015
start: 3326
end: 3454
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2518983
display id: annotation2518983
version: 1
top level: BBa_K2023015
location: https://synbiohub.org/public/igem/BBa_K2023015/annotation2518983/range2518983/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K2023015/annotation2518983/range2518983/1
type: Range
persistent identity: range2518983
display id: range2518983
version: 1
direction: direction/reverse
top level: BBa_K2023015
start: 2580
end: 2591
orientation: inline

GFP-PTS1
type: ComponentDefinition
persistent identity: BBa K678033
display id: BBa_K678033
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: GFP-PTS1
creator: DTU-Denmark-2
created: 2011-09-10T11:00:00Z
modified: 2015-05-08T01:13:04Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _882_
m user id: 0
owner id: 8341
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: GFP targeted to the peroxisomes of Aspergillus nidulans
mutable notes: Design considerations This part was designed for the Plug'n'Play with DNA assembly standard (RFC 80), therefore this part contains linkers at the 5' and 3' ends as shown on the figure below.
mutable provenance: to be announced
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: PTS1
sequence annotation: GFP
sequence annotation: Linker 2
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_K678033_sequence/1

PTS1
type: SequenceAnnotation
persistent identity: annotation2127397
display id: annotation2127397
version: 1
top level: GFP-PTS1
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K678033/annotation2127397/range2127397/1

https://synbiohub.org/public/igem/BBa_K678033/annotation2127397/range2127397/1
type: Range
persistent identity: range2127397
display id: range2127397
version: 1
direction: direction/forward
top level: GFP-PTS1
start: 726
end: 734
orientation: inline

GFP
type: SequenceAnnotation
persistent identity: annotation2127396
display id: annotation2127396
version: 1
top level: GFP-PTS1
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K678033/annotation2127396/range2127396/1

https://synbiohub.org/public/igem/BBa_K678033/annotation2127396/range2127396/1
type: Range
persistent identity: range2127396
display id: range2127396
version: 1
direction: direction/forward
top level: GFP-PTS1
start: 9
end: 725
orientation: inline

Linker 2
type: SequenceAnnotation
persistent identity: annotation2127395
display id: annotation2127395
version: 1
top level: GFP-PTS1
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678033/annotation2127395/range2127395/1

https://synbiohub.org/public/igem/BBa_K678033/annotation2127395/range2127395/1
type: Range
persistent identity: range2127395
display id: range2127395
version: 1
direction: direction/forward
top level: GFP-PTS1
start: 1
end: 8
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2127398
display id: annotation2127398
version: 1
top level: GFP-PTS1
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K678033/annotation2127398/range2127398/1

https://synbiohub.org/public/igem/BBa_K678033/annotation2127398/range2127398/1
type: Range
persistent identity: range2127398
display id: range2127398
version: 1
direction: direction/forward
top level: GFP-PTS1
start: 735
end: 737
orientation: inline

GFP
type: ComponentDefinition
persistent identity: BBa E0040
display id: BBa_E0040
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212
creator: jcbraff
created: 2004-09-29T11:00:00Z
modified: 2016-01-26T02:09:38Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _11_1_
m user id: 4206
owner id: 61
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: GFP (mut3b) [note that this part does not have a barcode]
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: GFP protein
sequence: https://synbiohub.org/public/igem/BBa_E0040_sequence/1

GFP protein
type: SequenceAnnotation
persistent identity: annotation1934520
display id: annotation1934520
version: 1
top level: GFP
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_E0040/annotation1934520/range1934520/1

https://synbiohub.org/public/igem/BBa_E0040/annotation1934520/range1934520/1
type: Range
persistent identity: range1934520
display id: range1934520
version: 1
direction: direction/forward
top level: GFP
start: 1
end: 720
orientation: inline

BBa_K1732022
type: ComponentDefinition
persistent identity: BBa K1732022
display id: BBa_K1732022
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: EGFPCO-His
creator: Donna Lee
created: 2015-09-17T11:00:00Z
modified: 2016-01-21T02:51:07Z
discontinued: false
dominant: false
group u list: _2154_
m user id: 4206
owner id: 27195
owning group id: 9
status: status/Planning
bookmark: false
mutable description: EGFP (BBa_1491002) with 6X-His tag for easier protein purification. Original sequence is codon optimized by IDT Codon Optimized Tool for E.coli.
mutable notes: Codon optimized for E.coli and His tag allows for easier protein purification.
mutable provenance: BBa_1491002
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: 6X-His
sequence annotation: EGFPCO
sequence: https://synbiohub.org/public/igem/BBa_K1732022_sequence/1

6X-His
type: SequenceAnnotation
persistent identity: annotation2469121
display id: annotation2469121
version: 1
top level: BBa_K1732022
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1732022/annotation2469121/range2469121/1

https://synbiohub.org/public/igem/BBa_K1732022/annotation2469121/range2469121/1
type: Range
persistent identity: range2469121
display id: range2469121
version: 1
direction: direction/forward
top level: BBa_K1732022
start: 717
end: 733
orientation: inline

EGFPCO
type: SequenceAnnotation
persistent identity: annotation2469080
display id: annotation2469080
version: 1
top level: BBa_K1732022
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1732022/annotation2469080/range2469080/1

https://synbiohub.org/public/igem/BBa_K1732022/annotation2469080/range2469080/1
type: Range
persistent identity: range2469080
display id: range2469080
version: 1
direction: direction/forward
top level: BBa_K1732022
start: 1
end: 716
orientation: inline

BBa_K1833002
type: ComponentDefinition
persistent identity: BBa K1833002
display id: BBa_K1833002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pT3-GFP
creator: Konstantin Borisov
created: 2015-09-15T11:00:00Z
modified: 2015-09-16T07:55:27Z
discontinued: false
dominant: false
group u list: _2259_
m user id: 27388
owner id: 27388
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This part produces GFP in the presence of T3 RNA polymerase. It contains a T3 promoter, a strong RBS, the coding region for GFP, and a double terminator. The GFP is a mutant known as GFPmut3b, and the original citation is as follows: Cormack, B.P., Valdivia, R.H., and S. Falkow. FACS-optimized mutants of green fluorescent protein (GFP). Gene 173: 33-38 (1996). (http://www.sciencedirect.com/science/article/pii/0378111995006850) It has a maximum excitation at 501 nm and maximum emission at 511 nm. For more information, see part BBa_E0040.
mutable notes: The part containing GFP was cut with EcoRI and XbaI, and ligated with the annealed oligos to form the completed part. Note that due to the choice of cloning technique, a Biobrick scar site remains between the promoter and RBS.
mutable provenance: The RBS-GFP-terminator was obtained from as part BBa_E0840 from the 2015 Distribution (Plate 2, Well 24D). The T3 promoter was obtained as two short oligonucleotides annealed to form sticky ends. The part containing GFP was then cut with EcoRI and XbaI, and ligated with the annealed oligos to form the completed part.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_E0840
component: BBa_K1833997
sequence annotation: BBa_E0840
sequence annotation: BBa_K1833997
sequence: https://synbiohub.org/public/igem/BBa_K1833002_sequence/1

BBa_E0840
type: Component
persistent identity: component2460323
display id: component2460323
version: 1
top level: BBa_K1833002
access: public
definition: https://synbiohub.org/public/igem/BBa_E0840/1

BBa_K1833997
type: Component
persistent identity: component2460312
display id: component2460312
version: 1
top level: BBa_K1833002
access: public
definition: https://synbiohub.org/public/igem/BBa_K1833997/1

BBa_E0840
type: SequenceAnnotation
persistent identity: annotation2460323
display id: annotation2460323
version: 1
top level: BBa_K1833002
location: https://synbiohub.org/public/igem/BBa_K1833002/annotation2460323/range2460323/1
component: BBa_E0840

https://synbiohub.org/public/igem/BBa_K1833002/annotation2460323/range2460323/1
type: Range
persistent identity: range2460323
display id: range2460323
version: 1
direction: direction/reverse
top level: BBa_K1833002
start: 30
end: 907
orientation: inline

BBa_K1833997
type: SequenceAnnotation
persistent identity: annotation2460312
display id: annotation2460312
version: 1
top level: BBa_K1833002
location: https://synbiohub.org/public/igem/BBa_K1833002/annotation2460312/range2460312/1
component: BBa_K1833997

https://synbiohub.org/public/igem/BBa_K1833002/annotation2460312/range2460312/1
type: Range
persistent identity: range2460312
display id: range2460312
version: 1
direction: direction/reverse
top level: BBa_K1833002
start: 1
end: 29
orientation: inline

BBa_K1399010
type: ComponentDefinition
persistent identity: BBa K1399010
display id: BBa_K1399010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Plac-RFP(AAV)
creator: Anna Stikane
created: 2014-09-18T11:00:00Z
modified: 2015-05-08T01:10:16Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1777_
m user id: 0
owner id: 22477
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: Lactose/IPTG inducible promoter wuth RFP reporter tagged with AAV-ssrA degradation tag followed by terminator. The tagged RFP is actively degraded within cell, thus provides better temporal resolution of red fluorescence and promoter activity. Part was assembled using BrickClip assembly (BBF RFC 104) using other biobrick parts as templates. BrickClip assembly is a special case of more general Paperclip assembly method.[1] Reference [1] Trubitsyna, M., Michlewski, G., Cai, Y., Elfick, A. & French, C. E. PaperClip: rapid multi-part DNA assembly from existing libraries. Nucleic Acids Res. 1???8 (2014). doi:10.1093/nar/gku829
mutable notes: no
mutable provenance: Other biobrick parts
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: stop
sequence annotation: BBa_R0010
sequence annotation: CAP binding site
sequence annotation: -35
sequence annotation: start
sequence annotation: LacI binding site
sequence annotation: stem_loop
sequence annotation: BBa_B0010
sequence annotation: end of LacI coding region (inactive)
sequence annotation: BBa_B0034
sequence annotation: conserved
sequence annotation: BBa_E1010 w/o stop
sequence annotation: barcode from BBa_E1010
sequence annotation: BBa_B0012
sequence annotation: BBa_K1399000
sequence annotation: BBa_B0015
sequence annotation: -10
sequence annotation: polya
sequence annotation: AAV-ssrA tag
sequence annotation: T7 TE
sequence: https://synbiohub.org/public/igem/BBa_K1399010_sequence/1

stop
type: SequenceAnnotation
persistent identity: annotation2383946
display id: annotation2383946
version: 1
top level: BBa_K1399010
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1399010/annotation2383946/range2383946/1

https://synbiohub.org/public/igem/BBa_K1399010/annotation2383946/range2383946/1
type: Range
persistent identity: range2383946
display id: range2383946
version: 1
direction: direction/forward
top level: BBa_K1399010
start: 938
end: 940
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation2383933
display id: annotation2383933
version: 1
top level: BBa_K1399010
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1399010/annotation2383933/range2383933/1

https://synbiohub.org/public/igem/BBa_K1399010/annotation2383933/range2383933/1
type: Range
persistent identity: range2383933
display id: range2383933
version: 1
direction: direction/forward
top level: BBa_K1399010
start: 1
end: 200
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2383953
display id: annotation2383953
version: 1
top level: BBa_K1399010
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1399010/annotation2383953/range2383953/1

https://synbiohub.org/public/igem/BBa_K1399010/annotation2383953/range2383953/1
type: Range
persistent identity: range2383953
display id: range2383953
version: 1
direction: direction/forward
top level: BBa_K1399010
start: 1095
end: 1095
orientation: inline

CAP binding site
type: SequenceAnnotation
persistent identity: annotation2383935
display id: annotation2383935
version: 1
top level: BBa_K1399010
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1399010/annotation2383935/range2383935/1

https://synbiohub.org/public/igem/BBa_K1399010/annotation2383935/range2383935/1
type: Range
persistent identity: range2383935
display id: range2383935
version: 1
direction: direction/forward
top level: BBa_K1399010
start: 89
end: 126
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2383936
display id: annotation2383936
version: 1
top level: BBa_K1399010
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1399010/annotation2383936/range2383936/1

https://synbiohub.org/public/igem/BBa_K1399010/annotation2383936/range2383936/1
type: Range
persistent identity: range2383936
display id: range2383936
version: 1
direction: direction/forward
top level: BBa_K1399010
start: 137
end: 142
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2383942
display id: annotation2383942
version: 1
top level: BBa_K1399010
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1399010/annotation2383942/range2383942/1

https://synbiohub.org/public/igem/BBa_K1399010/annotation2383942/range2383942/1
type: Range
persistent identity: range2383942
display id: range2383942
version: 1
direction: direction/forward
top level: BBa_K1399010
start: 227
end: 229
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2383945
display id: annotation2383945
version: 1
top level: BBa_K1399010
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1399010/annotation2383945/range2383945/1

https://synbiohub.org/public/igem/BBa_K1399010/annotation2383945/range2383945/1
type: Range
persistent identity: range2383945
display id: range2383945
version: 1
direction: direction/forward
top level: BBa_K1399010
start: 935
end: 937
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2383939
display id: annotation2383939
version: 1
top level: BBa_K1399010
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1399010/annotation2383939/range2383939/1

https://synbiohub.org/public/igem/BBa_K1399010/annotation2383939/range2383939/1
type: Range
persistent identity: range2383939
display id: range2383939
version: 1
direction: direction/forward
top level: BBa_K1399010
start: 173
end: 173
orientation: inline

LacI binding site
type: SequenceAnnotation
persistent identity: annotation2383938
display id: annotation2383938
version: 1
top level: BBa_K1399010
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1399010/annotation2383938/range2383938/1

https://synbiohub.org/public/igem/BBa_K1399010/annotation2383938/range2383938/1
type: Range
persistent identity: range2383938
display id: range2383938
version: 1
direction: direction/forward
top level: BBa_K1399010
start: 166
end: 200
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation2383949
display id: annotation2383949
version: 1
top level: BBa_K1399010
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1399010/annotation2383949/range2383949/1

https://synbiohub.org/public/igem/BBa_K1399010/annotation2383949/range2383949/1
type: Range
persistent identity: range2383949
display id: range2383949
version: 1
direction: direction/forward
top level: BBa_K1399010
start: 985
end: 1028
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2383948
display id: annotation2383948
version: 1
top level: BBa_K1399010
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1399010/annotation2383948/range2383948/1

https://synbiohub.org/public/igem/BBa_K1399010/annotation2383948/range2383948/1
type: Range
persistent identity: range2383948
display id: range2383948
version: 1
direction: direction/forward
top level: BBa_K1399010
start: 974
end: 1053
orientation: inline

end of LacI coding region (inactive)
type: SequenceAnnotation
persistent identity: annotation2383934
display id: annotation2383934
version: 1
top level: BBa_K1399010
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1399010/annotation2383934/range2383934/1

https://synbiohub.org/public/igem/BBa_K1399010/annotation2383934/range2383934/1
type: Range
persistent identity: range2383934
display id: range2383934
version: 1
direction: direction/forward
top level: BBa_K1399010
start: 1
end: 88
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2383941
display id: annotation2383941
version: 1
top level: BBa_K1399010
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1399010/annotation2383941/range2383941/1

https://synbiohub.org/public/igem/BBa_K1399010/annotation2383941/range2383941/1
type: Range
persistent identity: range2383941
display id: range2383941
version: 1
direction: direction/reverse
top level: BBa_K1399010
start: 209
end: 220
orientation: inline

conserved
type: SequenceAnnotation
persistent identity: annotation2383940
display id: annotation2383940
version: 1
top level: BBa_K1399010
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K1399010/annotation2383940/range2383940/1

https://synbiohub.org/public/igem/BBa_K1399010/annotation2383940/range2383940/1
type: Range
persistent identity: range2383940
display id: range2383940
version: 1
direction: direction/forward
top level: BBa_K1399010
start: 213
end: 216
orientation: inline

BBa_E1010 w/o stop
type: SequenceAnnotation
persistent identity: annotation2383943
display id: annotation2383943
version: 1
top level: BBa_K1399010
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1399010/annotation2383943/range2383943/1

https://synbiohub.org/public/igem/BBa_K1399010/annotation2383943/range2383943/1
type: Range
persistent identity: range2383943
display id: range2383943
version: 1
direction: direction/forward
top level: BBa_K1399010
start: 230
end: 901
orientation: inline

barcode from BBa_E1010
type: SequenceAnnotation
persistent identity: annotation2386257
display id: annotation2386257
version: 1
top level: BBa_K1399010
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1399010/annotation2386257/range2386257/1

https://synbiohub.org/public/igem/BBa_K1399010/annotation2386257/range2386257/1
type: Range
persistent identity: range2386257
display id: range2386257
version: 1
direction: direction/forward
top level: BBa_K1399010
start: 941
end: 965
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2383950
display id: annotation2383950
version: 1
top level: BBa_K1399010
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1399010/annotation2383950/range2383950/1

https://synbiohub.org/public/igem/BBa_K1399010/annotation2383950/range2383950/1
type: Range
persistent identity: range2383950
display id: range2383950
version: 1
direction: direction/forward
top level: BBa_K1399010
start: 1062
end: 1102
orientation: inline

BBa_K1399000
type: SequenceAnnotation
persistent identity: annotation2383947
display id: annotation2383947
version: 1
top level: BBa_K1399010
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1399010/annotation2383947/range2383947/1

https://synbiohub.org/public/igem/BBa_K1399010/annotation2383947/range2383947/1
type: Range
persistent identity: range2383947
display id: range2383947
version: 1
direction: direction/reverse
top level: BBa_K1399010
start: 227
end: 940
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2383954
display id: annotation2383954
version: 1
top level: BBa_K1399010
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1399010/annotation2383954/range2383954/1

https://synbiohub.org/public/igem/BBa_K1399010/annotation2383954/range2383954/1
type: Range
persistent identity: range2383954
display id: range2383954
version: 1
direction: direction/reverse
top level: BBa_K1399010
start: 974
end: 1102
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2383937
display id: annotation2383937
version: 1
top level: BBa_K1399010
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1399010/annotation2383937/range2383937/1

https://synbiohub.org/public/igem/BBa_K1399010/annotation2383937/range2383937/1
type: Range
persistent identity: range2383937
display id: range2383937
version: 1
direction: direction/forward
top level: BBa_K1399010
start: 161
end: 166
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation2383952
display id: annotation2383952
version: 1
top level: BBa_K1399010
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_K1399010/annotation2383952/range2383952/1

https://synbiohub.org/public/igem/BBa_K1399010/annotation2383952/range2383952/1
type: Range
persistent identity: range2383952
display id: range2383952
version: 1
direction: direction/forward
top level: BBa_K1399010
start: 1089
end: 1102
orientation: inline

AAV-ssrA tag
type: SequenceAnnotation
persistent identity: annotation2383944
display id: annotation2383944
version: 1
top level: BBa_K1399010
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1399010/annotation2383944/range2383944/1

https://synbiohub.org/public/igem/BBa_K1399010/annotation2383944/range2383944/1
type: Range
persistent identity: range2383944
display id: range2383944
version: 1
direction: direction/forward
top level: BBa_K1399010
start: 902
end: 934
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation2383951
display id: annotation2383951
version: 1
top level: BBa_K1399010
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1399010/annotation2383951/range2383951/1

https://synbiohub.org/public/igem/BBa_K1399010/annotation2383951/range2383951/1
type: Range
persistent identity: range2383951
display id: range2383951
version: 1
direction: direction/forward
top level: BBa_K1399010
start: 1069
end: 1088
orientation: inline

BBa_K1159302
type: ComponentDefinition
persistent identity: BBa K1159302
display id: BBa_K1159302
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Enhanced Cyan Fluorescent Protein (eCFP) in RFC[25]
creator: Dong-Jiunn Jeffery TRUONG
created: 2013-09-13T11:00:00Z
modified: 2016-01-26T03:03:36Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1471_
m user id: 4206
owner id: 11507
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This Biobricks is a improved version BBa_E0020 which codes for the Enhanced Cyan Fluorescent Protein. We improve the function of the biobrick, to allow protein fusions which is originally not possible due to RFC[10] pre- and suffix. This part is flanked by RFC[25] pre- and suffix for further protein fusions.
mutable notes: x
mutable provenance: ''Aequorea victoria''
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: eCFP
sequence: https://synbiohub.org/public/igem/BBa_K1159302_sequence/1

eCFP
type: SequenceAnnotation
persistent identity: annotation2343589
display id: annotation2343589
version: 1
top level: BBa_K1159302
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1159302/annotation2343589/range2343589/1

https://synbiohub.org/public/igem/BBa_K1159302/annotation2343589/range2343589/1
type: Range
persistent identity: range2343589
display id: range2343589
version: 1
direction: direction/forward
top level: BBa_K1159302
start: 1
end: 714
orientation: inline

dTomato
type: ComponentDefinition
persistent identity: BBa K1688004
display id: BBa_K1688004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: dTomato (inc RBS)
creator: Erik Gullberg
created: 2015-09-14T11:00:00Z
modified: 2015-09-18T10:34:03Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2106_
m user id: 27118
owner id: 27121
owning group id: 9
status: status/Planning
bookmark: false
mutable description: The dTomato protein is a fluorescent dimer protein that emits orange-red light when it is excited by green-yellow light. It is preferable to use ??? especially in self-made fluorometry tests ??? because the excitation wavelengths and the emission wavelengths don't overlap as much as in other fluorescent proteins. The dTomato excitation peak is at 554 nm and 50% of it is at 510 nm. Also, its emission peak is at 581 nm and its 50% emission at 629 nm (Figure 1). Characterization has been made with the Anderson promoters BBa_J23101 and (construct BBa_K1688010) and BBa_J23110 (construct BBa_K1688011).
mutable provenance: It was derived from a monomeric mutant of the anthozoan coral RFP, DsRed
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_K1688019
component: BBa_B0034
sequence annotation: BBa_K1688019
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K1688004_sequence/1

BBa_K1688019
type: Component
persistent identity: component2457405
display id: component2457405
version: 1
top level: dTomato
access: public
definition: dTomato

BBa_B0034
type: Component
persistent identity: component2457404
display id: component2457404
version: 1
top level: dTomato
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K1688019
type: SequenceAnnotation
persistent identity: annotation2457405
display id: annotation2457405
version: 1
top level: dTomato
location: https://synbiohub.org/public/igem/BBa_K1688004/annotation2457405/range2457405/1
component: BBa_K1688019

https://synbiohub.org/public/igem/BBa_K1688004/annotation2457405/range2457405/1
type: Range
persistent identity: range2457405
display id: range2457405
version: 1
direction: direction/reverse
top level: dTomato
start: 19
end: 726
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2457404
display id: annotation2457404
version: 1
top level: dTomato
location: https://synbiohub.org/public/igem/BBa_K1688004/annotation2457404/range2457404/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1688004/annotation2457404/range2457404/1
type: Range
persistent identity: range2457404
display id: range2457404
version: 1
direction: direction/reverse
top level: dTomato
start: 1
end: 12
orientation: inline

CFP
type: ComponentDefinition
persistent identity: BBa K566004
display id: BBa_K566004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CFP protein generator without transcription terminator
creator: Daniel Rodriguez
created: 2011-09-25T11:00:00Z
modified: 2015-05-08T01:12:42Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _734_
m user id: 0
owner id: 8650
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: Protein generator of cyan fluorescent protein without transcription terminator. It may be negative regulated by Mnt repressor (BBa_C0072).
mutable notes: Codon optimization for better expression in E. coli. Illegal sites were removed.
mutable provenance: Synthesized DNA.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_R0073
component: BBa_B0034
component: BBa_K566003
sequence annotation: BBa_R0073
sequence annotation: BBa_B0034
sequence annotation: BBa_K566003
sequence: https://synbiohub.org/public/igem/BBa_K566004_sequence/1

BBa_R0073
type: Component
persistent identity: component2142652
display id: component2142652
version: 1
top level: CFP
access: public
definition: https://synbiohub.org/public/igem/BBa_R0073/1

BBa_B0034
type: Component
persistent identity: component2142654
display id: component2142654
version: 1
top level: CFP
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K566003
type: Component
persistent identity: component2142659
display id: component2142659
version: 1
top level: CFP
access: public
definition: https://synbiohub.org/public/igem/BBa_K566003/1

BBa_R0073
type: SequenceAnnotation
persistent identity: annotation2142652
display id: annotation2142652
version: 1
top level: CFP
location: https://synbiohub.org/public/igem/BBa_K566004/annotation2142652/range2142652/1
component: BBa_R0073

https://synbiohub.org/public/igem/BBa_K566004/annotation2142652/range2142652/1
type: Range
persistent identity: range2142652
display id: range2142652
version: 1
direction: direction/reverse
top level: CFP
start: 1
end: 67
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2142654
display id: annotation2142654
version: 1
top level: CFP
location: https://synbiohub.org/public/igem/BBa_K566004/annotation2142654/range2142654/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K566004/annotation2142654/range2142654/1
type: Range
persistent identity: range2142654
display id: range2142654
version: 1
direction: direction/reverse
top level: CFP
start: 68
end: 79
orientation: inline

BBa_K566003
type: SequenceAnnotation
persistent identity: annotation2142659
display id: annotation2142659
version: 1
top level: CFP
location: https://synbiohub.org/public/igem/BBa_K566004/annotation2142659/range2142659/1
component: BBa_K566003

https://synbiohub.org/public/igem/BBa_K566004/annotation2142659/range2142659/1
type: Range
persistent identity: range2142659
display id: range2142659
version: 1
direction: direction/reverse
top level: CFP
start: 80
end: 841
orientation: inline

BBa_K2023014
type: ComponentDefinition
persistent identity: BBa K2023014
display id: BBa_K2023014
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: GFP coding device with Pr
creator: C??lia Chenebault
created: 2016-10-13T11:00:00Z
modified: 2016-10-20T03:26:35Z
discontinued: false
dominant: false
group u list: _2490_
m user id: 30356
owner id: 30230
owning group id: 9
status: status/Planning
bookmark: false
mutable description: GFP coding device with Pr
mutable notes: Blabla
mutable provenance: Digestion/ligation of Pr-RBS with GFP reporter (BBa_I13401)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_B0010
component: BBa_E0040
component: BBa_I723018
component: BBa_B0034
component: BBa_B0012
sequence annotation: BBa_I723018
sequence annotation: BBa_B0034
sequence annotation: BBa_E0040
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_K2023014_sequence/1

BBa_B0010
type: Component
persistent identity: component2505710
display id: component2505710
version: 1
top level: BBa_K2023014
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_E0040
type: Component
persistent identity: component2505709
display id: component2505709
version: 1
top level: BBa_K2023014
access: public
definition: GFP

BBa_I723018
type: Component
persistent identity: component2505705
display id: component2505705
version: 1
top level: BBa_K2023014
access: public
definition: https://synbiohub.org/public/igem/BBa_I723018/1

BBa_B0034
type: Component
persistent identity: component2505707
display id: component2505707
version: 1
top level: BBa_K2023014
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0012
type: Component
persistent identity: component2505712
display id: component2505712
version: 1
top level: BBa_K2023014
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_I723018
type: SequenceAnnotation
persistent identity: annotation2505705
display id: annotation2505705
version: 1
top level: BBa_K2023014
location: https://synbiohub.org/public/igem/BBa_K2023014/annotation2505705/range2505705/1
component: BBa_I723018

https://synbiohub.org/public/igem/BBa_K2023014/annotation2505705/range2505705/1
type: Range
persistent identity: range2505705
display id: range2505705
version: 1
direction: direction/reverse
top level: BBa_K2023014
start: 1
end: 410
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2505707
display id: annotation2505707
version: 1
top level: BBa_K2023014
location: https://synbiohub.org/public/igem/BBa_K2023014/annotation2505707/range2505707/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K2023014/annotation2505707/range2505707/1
type: Range
persistent identity: range2505707
display id: range2505707
version: 1
direction: direction/reverse
top level: BBa_K2023014
start: 419
end: 430
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation2505709
display id: annotation2505709
version: 1
top level: BBa_K2023014
location: https://synbiohub.org/public/igem/BBa_K2023014/annotation2505709/range2505709/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_K2023014/annotation2505709/range2505709/1
type: Range
persistent identity: range2505709
display id: range2505709
version: 1
direction: direction/reverse
top level: BBa_K2023014
start: 437
end: 1156
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2505710
display id: annotation2505710
version: 1
top level: BBa_K2023014
location: https://synbiohub.org/public/igem/BBa_K2023014/annotation2505710/range2505710/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K2023014/annotation2505710/range2505710/1
type: Range
persistent identity: range2505710
display id: range2505710
version: 1
direction: direction/forward
top level: BBa_K2023014
start: 1165
end: 1244
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2505712
display id: annotation2505712
version: 1
top level: BBa_K2023014
location: https://synbiohub.org/public/igem/BBa_K2023014/annotation2505712/range2505712/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K2023014/annotation2505712/range2505712/1
type: Range
persistent identity: range2505712
display id: range2505712
version: 1
direction: direction/forward
top level: BBa_K2023014
start: 1253
end: 1293
orientation: inline

RFP2
type: ComponentDefinition
persistent identity: BBa I715023
display id: BBa_I715023
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Carboxyl portion of RFP
creator: Andrew Martens
created: 2007-06-24T11:00:00Z
modified: 2016-01-25T04:44:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _120_
m user id: 4206
owner id: 201
owning group id: 61
sample status: In stock
status: status/Available
bookmark: false
mutable description: The second portion of the RFP gene, after the HixC insertion point.
mutable notes: Used web tool.
mutable provenance: Sequence from part BBa_E1010.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: (two in tandem)
sequence annotation: extra base (avoid frameshift)
sequence annotation: Continue coding sequence
sequence: https://synbiohub.org/public/igem/BBa_I715023_sequence/1

(two in tandem)
type: SequenceAnnotation
persistent identity: annotation1935384
display id: annotation1935384
version: 1
top level: RFP2
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_I715023/annotation1935384/range1935384/1

https://synbiohub.org/public/igem/BBa_I715023/annotation1935384/range1935384/1
type: Range
persistent identity: range1935384
display id: range1935384
version: 1
direction: direction/forward
top level: RFP2
start: 215
end: 219
orientation: inline

extra base (avoid frameshift)
type: SequenceAnnotation
persistent identity: annotation1935382
display id: annotation1935382
version: 1
top level: RFP2
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I715023/annotation1935382/range1935382/1

https://synbiohub.org/public/igem/BBa_I715023/annotation1935382/range1935382/1
type: Range
persistent identity: range1935382
display id: range1935382
version: 1
direction: direction/forward
top level: RFP2
start: 1
end: 1
orientation: inline

Continue coding sequence
type: SequenceAnnotation
persistent identity: annotation1935383
display id: annotation1935383
version: 1
top level: RFP2
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_I715023/annotation1935383/range1935383/1

https://synbiohub.org/public/igem/BBa_I715023/annotation1935383/range1935383/1
type: Range
persistent identity: range1935383
display id: range1935383
version: 1
direction: direction/forward
top level: RFP2
start: 2
end: 214
orientation: inline

eyfp
type: ComponentDefinition
persistent identity: BBa E0031
display id: BBa_E0031
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: EYFP (RBS+ LVA-)
creator: Caitlin Conboy and Jennifer Braff
created: 2004-04-21T11:00:00Z
modified: 2015-08-31T04:07:25Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: EYFP with Clontech RBS, no degradation tail
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
sequence annotation: stop
sequence annotation: Clontech RBS
sequence annotation: EYFP
sequence: https://synbiohub.org/public/igem/BBa_E0031_sequence/1

stop
type: SequenceAnnotation
persistent identity: annotation353785
display id: annotation353785
version: 1
top level: eyfp
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_E0031/annotation353785/range353785/1

https://synbiohub.org/public/igem/BBa_E0031/annotation353785/range353785/1
type: Range
persistent identity: range353785
display id: range353785
version: 1
direction: direction/forward
top level: eyfp
start: 737
end: 739
orientation: inline

Clontech RBS
type: SequenceAnnotation
persistent identity: annotation353780
display id: annotation353780
version: 1
top level: eyfp
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_E0031/annotation353780/range353780/1

https://synbiohub.org/public/igem/BBa_E0031/annotation353780/range353780/1
type: Range
persistent identity: range353780
display id: range353780
version: 1
direction: direction/forward
top level: eyfp
start: 2
end: 20
orientation: inline

EYFP
type: SequenceAnnotation
persistent identity: annotation353784
display id: annotation353784
version: 1
top level: eyfp
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_E0031/annotation353784/range353784/1

https://synbiohub.org/public/igem/BBa_E0031/annotation353784/range353784/1
type: Range
persistent identity: range353784
display id: range353784
version: 1
direction: direction/forward
top level: eyfp
start: 21
end: 736
orientation: inline

BBa_K1323010
type: ComponentDefinition
persistent identity: BBa K1323010
display id: BBa_K1323010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: YFP Coding Sequence
creator: Julia Manalil
created: 2014-10-03T11:00:00Z
modified: 2016-01-26T02:08:54Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1698_
m user id: 4206
owner id: 18368
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: TBA
mutable notes: TBA
mutable provenance: TBA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: YFP
sequence: https://synbiohub.org/public/igem/BBa_K1323010_sequence/1

YFP
type: SequenceAnnotation
persistent identity: annotation2428779
display id: annotation2428779
version: 1
top level: BBa_K1323010
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1323010/annotation2428779/range2428779/1

https://synbiohub.org/public/igem/BBa_K1323010/annotation2428779/range2428779/1
type: Range
persistent identity: range2428779
display id: range2428779
version: 1
direction: direction/forward
top level: BBa_K1323010
start: 1
end: 720
orientation: inline

BBa_J52021
type: ComponentDefinition
persistent identity: BBa J52021
display id: BBa_J52021
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: dnTraf6-linker-GFP
creator: Matej Skocaj
created: 2006-10-13T11:00:00Z
modified: 2016-01-26T09:38:59Z
discontinued: false
dominant: false
experience: experience/None
group u list: _80_
m user id: 0
m user id: 4206
owner id: 857
owning group id: 80
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Part is composed of TIR domain of Traf6 and GFP linked with 6 aminoacids long linker. GFP serves as reporter. For usage in eukaryotes.
mutable notes: None.
mutable provenance: dnTraf6- pDeNy GFP-
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: GFP
sequence annotation: dnTraf6-linker-GFP
sequence annotation: linker
sequence annotation: dnTraf6
sequence: https://synbiohub.org/public/igem/BBa_J52021_sequence/1

GFP
type: SequenceAnnotation
persistent identity: annotation1903261
display id: annotation1903261
version: 1
top level: BBa_J52021
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J52021/annotation1903261/range1903261/1

https://synbiohub.org/public/igem/BBa_J52021/annotation1903261/range1903261/1
type: Range
persistent identity: range1903261
display id: range1903261
version: 1
direction: direction/forward
top level: BBa_J52021
start: 733
end: 1446
orientation: inline

dnTraf6-linker-GFP
type: SequenceAnnotation
persistent identity: annotation1903262
display id: annotation1903262
version: 1
top level: BBa_J52021
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_J52021/annotation1903262/range1903262/1

https://synbiohub.org/public/igem/BBa_J52021/annotation1903262/range1903262/1
type: Range
persistent identity: range1903262
display id: range1903262
version: 1
direction: direction/forward
top level: BBa_J52021
start: 1
end: 1446
orientation: inline

linker
type: SequenceAnnotation
persistent identity: annotation1903260
display id: annotation1903260
version: 1
top level: BBa_J52021
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_J52021/annotation1903260/range1903260/1

https://synbiohub.org/public/igem/BBa_J52021/annotation1903260/range1903260/1
type: Range
persistent identity: range1903260
display id: range1903260
version: 1
direction: direction/forward
top level: BBa_J52021
start: 715
end: 732
orientation: inline

dnTraf6
type: SequenceAnnotation
persistent identity: annotation1903259
display id: annotation1903259
version: 1
top level: BBa_J52021
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J52021/annotation1903259/range1903259/1

https://synbiohub.org/public/igem/BBa_J52021/annotation1903259/range1903259/1
type: Range
persistent identity: range1903259
display id: range1903259
version: 1
direction: direction/forward
top level: BBa_J52021
start: 1
end: 714
orientation: inline

BBa_K648013
type: ComponentDefinition
persistent identity: BBa K648013
display id: BBa_K648013
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: GFP with Standard 25 Prefix/Suffix
creator: Jim Rose
created: 2011-07-03T11:00:00Z
modified: 2016-01-25T02:32:21Z
discontinued: false
dominant: false
group u list: _825_
m user id: 4206
owner id: 9871
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This is the standard GFP protein reporter (E0040) cloned with the prefix/suffix required for standard 25 assembly. It can easily be used to create fusion proteins through this method.
mutable notes: This part contains the AgeI and NgoMIV sites as part of it's prefix/suffix that allows it to be used in standard 25 assembly. It was synthesized through PCR oligo synthesis methods using the following primers: Forward (56.88 degrees) TATTGAATTCGCGGCCGCTTCTAGATGGCCGGCCGTAAAGGAGAAGAACTTTTC Reverse (56.95 degrees) AATACTGCAGCGGCCGCTACTAGTATTAACCGGTCTTGTCGTCATCATCTTTATAAT
mutable provenance: This part is similar to the part BBa_E0040 commonly used as a reporter.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: misc
sequence: https://synbiohub.org/public/igem/BBa_K648013_sequence/1

misc
type: SequenceAnnotation
persistent identity: annotation2122827
display id: annotation2122827
version: 1
top level: BBa_K648013
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K648013/annotation2122827/range2122827/1

https://synbiohub.org/public/igem/BBa_K648013/annotation2122827/range2122827/1
type: Range
persistent identity: range2122827
display id: range2122827
version: 1
direction: direction/forward
top level: BBa_K648013
start: 1
end: 735
orientation: inline

BBa_K1140006
type: ComponentDefinition
persistent identity: BBa K1140006
display id: BBa_K1140006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pTet + 37 oC RNA thermometer + mCherry (LVA)
creator: Janssel Reyes del Castillo
created: 2013-09-12T11:00:00Z
modified: 2015-05-08T01:09:23Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1452_
m user id: 0
owner id: 12878
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This part contains a promoter regulated by TetR protein. The Transcription is regulated by a 37 oC riboswitch. The protein codified is a RFP with a LVA tag.
mutable notes: The prefix sites are EcoRI and XbaI and the sufix sites are SpeI and PstI.
mutable provenance: Obtained synthetically. Promoter pTet. Based on a fusion of a RBS and a Riboswitch. RFP with a LVA tag (Further information coming soon).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: mCherry+LVA
sequence annotation: U6 RNA thermometer
sequence annotation: t7 terminator
sequence annotation: pTet
sequence: https://synbiohub.org/public/igem/BBa_K1140006_sequence/1

mCherry+LVA
type: SequenceAnnotation
persistent identity: annotation2341071
display id: annotation2341071
version: 1
top level: BBa_K1140006
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1140006/annotation2341071/range2341071/1

https://synbiohub.org/public/igem/BBa_K1140006/annotation2341071/range2341071/1
type: Range
persistent identity: range2341071
display id: range2341071
version: 1
direction: direction/forward
top level: BBa_K1140006
start: 122
end: 835
orientation: inline

U6 RNA thermometer
type: SequenceAnnotation
persistent identity: annotation2341070
display id: annotation2341070
version: 1
top level: BBa_K1140006
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1140006/annotation2341070/range2341070/1

https://synbiohub.org/public/igem/BBa_K1140006/annotation2341070/range2341070/1
type: Range
persistent identity: range2341070
display id: range2341070
version: 1
direction: direction/forward
top level: BBa_K1140006
start: 63
end: 121
orientation: inline

t7 terminator
type: SequenceAnnotation
persistent identity: annotation2341072
display id: annotation2341072
version: 1
top level: BBa_K1140006
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1140006/annotation2341072/range2341072/1

https://synbiohub.org/public/igem/BBa_K1140006/annotation2341072/range2341072/1
type: Range
persistent identity: range2341072
display id: range2341072
version: 1
direction: direction/forward
top level: BBa_K1140006
start: 844
end: 887
orientation: inline

pTet
type: SequenceAnnotation
persistent identity: annotation2341069
display id: annotation2341069
version: 1
top level: BBa_K1140006
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1140006/annotation2341069/range2341069/1

https://synbiohub.org/public/igem/BBa_K1140006/annotation2341069/range2341069/1
type: Range
persistent identity: range2341069
display id: range2341069
version: 1
direction: direction/forward
top level: BBa_K1140006
start: 1
end: 54
orientation: inline

BBa_K1486039
type: ComponentDefinition
persistent identity: BBa K1486039
display id: BBa_K1486039
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: sfGFPC
creator: EPFL iGem team 2014
created: 2014-09-26T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: To edit
mutable notes: To edit
mutable provenance: To edit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence: https://synbiohub.org/public/igem/BBa_K1486039_sequence/1

BBa_J97002
type: ComponentDefinition
persistent identity: BBa J97002
display id: BBa_J97002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: IP-Free BlazeYFP (Yellow Fluorescent Protein)
creator: Drew Endy
created: 2014-02-18T12:00:00Z
modified: 2015-05-08T01:08:32Z
discontinued: false
dominant: false
experience: experience/None
group u list: _41_1_
m user id: 0
owner id: 747
owning group id: 63
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This part was created by DNA 2.0 as part of their IP-Free series of fluorescent and chromogenic proteins. It is available to use under the BioBrick Public Agreement. Excitation 515nm, Emission 534nm Molecular Weight: 29.2kDA Length: 263aa
mutable notes: This sequence is fully synthetic, (non-Aequorea) and is codon optimized for expression in E. coli.
mutable provenance: This synthetic part was created through a gene engineering and synthesis project at DNA 2.0.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence: https://synbiohub.org/public/igem/BBa_J97002_sequence/1

BBa_K1399013
type: ComponentDefinition
persistent identity: BBa K1399013
display id: BBa_K1399013
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Plac-RFP(DAS)
creator: Anna Stikane
created: 2014-09-18T11:00:00Z
modified: 2015-05-08T01:10:16Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1777_
m user id: 0
owner id: 22477
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: Lactose/IPTG inducible promoter with RFP reporter tagged with DAS-ssrA degradation tag followed by terminator. The tagged RFP is degraded within cell, thus provides better temporal resolution of red fluorescence and promoter activity. Presence of SspB is crucial for effective protein degradation.
mutable notes: Part was assembled using BrickClip assembly (BBF RFC104) using other biobrick parts as templates. BrickClip assembly is a special case of more general Paperclip assembly method.[1]
mutable provenance: other biobricks
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: polya
sequence annotation: BBa_R0010
sequence annotation: BBa_K1399003
sequence annotation: DAS-ssrA tag
sequence annotation: start
sequence annotation: LacI binding site
sequence annotation: -35
sequence annotation: stop
sequence annotation: BBa_B0034
sequence annotation: stem_loop
sequence annotation: barcode from BBa_E1010
sequence annotation: BBa_B0015
sequence annotation: T7 TE
sequence annotation: conserved
sequence annotation: CAP binding site
sequence annotation: BBa_B0012
sequence annotation: BBa_B0010
sequence annotation: end of LacI coding region (inactive)
sequence annotation: RFP
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_K1399013_sequence/1

polya
type: SequenceAnnotation
persistent identity: annotation2384031
display id: annotation2384031
version: 1
top level: BBa_K1399013
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_K1399013/annotation2384031/range2384031/1

https://synbiohub.org/public/igem/BBa_K1399013/annotation2384031/range2384031/1
type: Range
persistent identity: range2384031
display id: range2384031
version: 1
direction: direction/forward
top level: BBa_K1399013
start: 1089
end: 1102
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation2384012
display id: annotation2384012
version: 1
top level: BBa_K1399013
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1399013/annotation2384012/range2384012/1

https://synbiohub.org/public/igem/BBa_K1399013/annotation2384012/range2384012/1
type: Range
persistent identity: range2384012
display id: range2384012
version: 1
direction: direction/forward
top level: BBa_K1399013
start: 1
end: 200
orientation: inline

BBa_K1399003
type: SequenceAnnotation
persistent identity: annotation2384026
display id: annotation2384026
version: 1
top level: BBa_K1399013
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1399013/annotation2384026/range2384026/1

https://synbiohub.org/public/igem/BBa_K1399013/annotation2384026/range2384026/1
type: Range
persistent identity: range2384026
display id: range2384026
version: 1
direction: direction/reverse
top level: BBa_K1399013
start: 227
end: 940
orientation: inline

DAS-ssrA tag
type: SequenceAnnotation
persistent identity: annotation2384023
display id: annotation2384023
version: 1
top level: BBa_K1399013
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1399013/annotation2384023/range2384023/1

https://synbiohub.org/public/igem/BBa_K1399013/annotation2384023/range2384023/1
type: Range
persistent identity: range2384023
display id: range2384023
version: 1
direction: direction/forward
top level: BBa_K1399013
start: 902
end: 934
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2384021
display id: annotation2384021
version: 1
top level: BBa_K1399013
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1399013/annotation2384021/range2384021/1

https://synbiohub.org/public/igem/BBa_K1399013/annotation2384021/range2384021/1
type: Range
persistent identity: range2384021
display id: range2384021
version: 1
direction: direction/forward
top level: BBa_K1399013
start: 227
end: 229
orientation: inline

LacI binding site
type: SequenceAnnotation
persistent identity: annotation2384017
display id: annotation2384017
version: 1
top level: BBa_K1399013
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1399013/annotation2384017/range2384017/1

https://synbiohub.org/public/igem/BBa_K1399013/annotation2384017/range2384017/1
type: Range
persistent identity: range2384017
display id: range2384017
version: 1
direction: direction/forward
top level: BBa_K1399013
start: 166
end: 200
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2384018
display id: annotation2384018
version: 1
top level: BBa_K1399013
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1399013/annotation2384018/range2384018/1

https://synbiohub.org/public/igem/BBa_K1399013/annotation2384018/range2384018/1
type: Range
persistent identity: range2384018
display id: range2384018
version: 1
direction: direction/forward
top level: BBa_K1399013
start: 173
end: 173
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2384015
display id: annotation2384015
version: 1
top level: BBa_K1399013
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1399013/annotation2384015/range2384015/1

https://synbiohub.org/public/igem/BBa_K1399013/annotation2384015/range2384015/1
type: Range
persistent identity: range2384015
display id: range2384015
version: 1
direction: direction/forward
top level: BBa_K1399013
start: 137
end: 142
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2384024
display id: annotation2384024
version: 1
top level: BBa_K1399013
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1399013/annotation2384024/range2384024/1

https://synbiohub.org/public/igem/BBa_K1399013/annotation2384024/range2384024/1
type: Range
persistent identity: range2384024
display id: range2384024
version: 1
direction: direction/forward
top level: BBa_K1399013
start: 935
end: 937
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2384020
display id: annotation2384020
version: 1
top level: BBa_K1399013
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1399013/annotation2384020/range2384020/1

https://synbiohub.org/public/igem/BBa_K1399013/annotation2384020/range2384020/1
type: Range
persistent identity: range2384020
display id: range2384020
version: 1
direction: direction/reverse
top level: BBa_K1399013
start: 209
end: 220
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation2384028
display id: annotation2384028
version: 1
top level: BBa_K1399013
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1399013/annotation2384028/range2384028/1

https://synbiohub.org/public/igem/BBa_K1399013/annotation2384028/range2384028/1
type: Range
persistent identity: range2384028
display id: range2384028
version: 1
direction: direction/forward
top level: BBa_K1399013
start: 985
end: 1028
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2384032
display id: annotation2384032
version: 1
top level: BBa_K1399013
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1399013/annotation2384032/range2384032/1

https://synbiohub.org/public/igem/BBa_K1399013/annotation2384032/range2384032/1
type: Range
persistent identity: range2384032
display id: range2384032
version: 1
direction: direction/forward
top level: BBa_K1399013
start: 1095
end: 1095
orientation: inline

barcode from BBa_E1010
type: SequenceAnnotation
persistent identity: annotation2386313
display id: annotation2386313
version: 1
top level: BBa_K1399013
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1399013/annotation2386313/range2386313/1

https://synbiohub.org/public/igem/BBa_K1399013/annotation2386313/range2386313/1
type: Range
persistent identity: range2386313
display id: range2386313
version: 1
direction: direction/forward
top level: BBa_K1399013
start: 941
end: 965
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2384033
display id: annotation2384033
version: 1
top level: BBa_K1399013
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1399013/annotation2384033/range2384033/1

https://synbiohub.org/public/igem/BBa_K1399013/annotation2384033/range2384033/1
type: Range
persistent identity: range2384033
display id: range2384033
version: 1
direction: direction/reverse
top level: BBa_K1399013
start: 974
end: 1102
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation2384030
display id: annotation2384030
version: 1
top level: BBa_K1399013
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1399013/annotation2384030/range2384030/1

https://synbiohub.org/public/igem/BBa_K1399013/annotation2384030/range2384030/1
type: Range
persistent identity: range2384030
display id: range2384030
version: 1
direction: direction/forward
top level: BBa_K1399013
start: 1069
end: 1088
orientation: inline

conserved
type: SequenceAnnotation
persistent identity: annotation2384019
display id: annotation2384019
version: 1
top level: BBa_K1399013
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K1399013/annotation2384019/range2384019/1

https://synbiohub.org/public/igem/BBa_K1399013/annotation2384019/range2384019/1
type: Range
persistent identity: range2384019
display id: range2384019
version: 1
direction: direction/forward
top level: BBa_K1399013
start: 213
end: 216
orientation: inline

CAP binding site
type: SequenceAnnotation
persistent identity: annotation2384014
display id: annotation2384014
version: 1
top level: BBa_K1399013
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1399013/annotation2384014/range2384014/1

https://synbiohub.org/public/igem/BBa_K1399013/annotation2384014/range2384014/1
type: Range
persistent identity: range2384014
display id: range2384014
version: 1
direction: direction/forward
top level: BBa_K1399013
start: 89
end: 126
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2384025
display id: annotation2384025
version: 1
top level: BBa_K1399013
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1399013/annotation2384025/range2384025/1

https://synbiohub.org/public/igem/BBa_K1399013/annotation2384025/range2384025/1
type: Range
persistent identity: range2384025
display id: range2384025
version: 1
direction: direction/forward
top level: BBa_K1399013
start: 938
end: 940
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2384029
display id: annotation2384029
version: 1
top level: BBa_K1399013
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1399013/annotation2384029/range2384029/1

https://synbiohub.org/public/igem/BBa_K1399013/annotation2384029/range2384029/1
type: Range
persistent identity: range2384029
display id: range2384029
version: 1
direction: direction/forward
top level: BBa_K1399013
start: 1062
end: 1102
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2384027
display id: annotation2384027
version: 1
top level: BBa_K1399013
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1399013/annotation2384027/range2384027/1

https://synbiohub.org/public/igem/BBa_K1399013/annotation2384027/range2384027/1
type: Range
persistent identity: range2384027
display id: range2384027
version: 1
direction: direction/forward
top level: BBa_K1399013
start: 974
end: 1053
orientation: inline

end of LacI coding region (inactive)
type: SequenceAnnotation
persistent identity: annotation2384013
display id: annotation2384013
version: 1
top level: BBa_K1399013
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1399013/annotation2384013/range2384013/1

https://synbiohub.org/public/igem/BBa_K1399013/annotation2384013/range2384013/1
type: Range
persistent identity: range2384013
display id: range2384013
version: 1
direction: direction/forward
top level: BBa_K1399013
start: 1
end: 88
orientation: inline

RFP
type: SequenceAnnotation
persistent identity: annotation2384022
display id: annotation2384022
version: 1
top level: BBa_K1399013
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1399013/annotation2384022/range2384022/1

https://synbiohub.org/public/igem/BBa_K1399013/annotation2384022/range2384022/1
type: Range
persistent identity: range2384022
display id: range2384022
version: 1
direction: direction/forward
top level: BBa_K1399013
start: 230
end: 901
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2384016
display id: annotation2384016
version: 1
top level: BBa_K1399013
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1399013/annotation2384016/range2384016/1

https://synbiohub.org/public/igem/BBa_K1399013/annotation2384016/range2384016/1
type: Range
persistent identity: range2384016
display id: range2384016
version: 1
direction: direction/forward
top level: BBa_K1399013
start: 161
end: 166
orientation: inline

BBa_K1078001
type: ComponentDefinition
persistent identity: BBa K1078001
display id: BBa_K1078001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Strong reporter device optimized for Pichia pastoris, activated by methanol
creator: Edgar Andres Ochoa Cruz
created: 2013-09-11T11:00:00Z
modified: 2015-05-08T01:09:03Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1387_
m user id: 0
owner id: 11298
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: This device is composed by three parts. The first part is a modified promoter pAOX, it was found in a library where it showed more than 60% stronger activation when compared with the wild-type, as reported by Hartner FS, Ruth C, Langenegger D, Johnson SN, Hyka P, Lin-Cereghino GP, Lin-Cereghino J, Kovar K, Cregg JM, Glieder A: Promoter library designed for fine-tuned gene expression in Pichia pastoris. Nucleic Acids Res 2008, 36:e76. The second part is the kozak sequence for Pichia pastoris, and the third is a Red fluorescent protein condon optimized for expression in Pichia pastoris. This device was created to express a reporter gene in the presence of ethanol, and is inactivated by ethanol, when combined with the modified Mxr1 (BBa_K1078000), it is not inactivated by ethanol. This is useful for our biosensor design, which aims to detect levels of methanol above 2% in common alcoholic drinks (normal content of 10 to 60% ethanol). This will allow government to make high-throughput screening of ethanol drinks tainted will methanol. The modified Mxr1 is able to activate the pAOX promoter, in ethanol, glycerol and methanol. This is useful for our biosensor design, which aims to detect levels of methanol above 2% in common alcoholic drinks (normal content of 10 to 60% ethanol). This will allow government to make high-throughput screening of ethanol drinks tainted will methanol.
mutable notes: After synthesis, in order to build our sensor the part was introduced in pPIC9K plasmid to allow its genomic recombination into the Pichia pastoris genome, substituting the original Mxr1 found in the yeast. This part does not have a single biobrick version with the Mx1p because both must be inserted in different genome locations at the Pichia pastoris
mutable provenance: he part was obtained by synthesis, the modified Mxr1p sequence was found in Hartner FS, Ruth C, Langenegger D, Johnson SN, Hyka P, Lin-Cereghino GP, Lin-Cereghino J, Kovar K, Cregg JM, Glieder A: Promoter library designed for fine-tuned gene expression in Pichia pastoris. Nucleic Acids Res 2008, 36:e76
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
sequence annotation: RFP modified
sequence annotation: Kozak sequence
sequence annotation: pAOX modified
sequence: https://synbiohub.org/public/igem/BBa_K1078001_sequence/1

RFP modified
type: SequenceAnnotation
persistent identity: annotation2339072
display id: annotation2339072
version: 1
top level: BBa_K1078001
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1078001/annotation2339072/range2339072/1

https://synbiohub.org/public/igem/BBa_K1078001/annotation2339072/range2339072/1
type: Range
persistent identity: range2339072
display id: range2339072
version: 1
direction: direction/forward
top level: BBa_K1078001
start: 900
end: 1577
orientation: inline

Kozak sequence
type: SequenceAnnotation
persistent identity: annotation2339071
display id: annotation2339071
version: 1
top level: BBa_K1078001
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1078001/annotation2339071/range2339071/1

https://synbiohub.org/public/igem/BBa_K1078001/annotation2339071/range2339071/1
type: Range
persistent identity: range2339071
display id: range2339071
version: 1
direction: direction/forward
top level: BBa_K1078001
start: 888
end: 899
orientation: inline

pAOX modified
type: SequenceAnnotation
persistent identity: annotation2339065
display id: annotation2339065
version: 1
top level: BBa_K1078001
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1078001/annotation2339065/range2339065/1

https://synbiohub.org/public/igem/BBa_K1078001/annotation2339065/range2339065/1
type: Range
persistent identity: range2339065
display id: range2339065
version: 1
direction: direction/forward
top level: BBa_K1078001
start: 1
end: 887
orientation: inline

BBa_K1491001
type: ComponentDefinition
persistent identity: BBa K1491001
display id: BBa_K1491001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: mTagBFP-His
creator: Danielle Peters
created: 2014-07-14T11:00:00Z
modified: 2016-01-21T03:45:43Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1871_
m user id: 4206
owner id: 22521
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: mTagBFP with 6x His Tag for protein purification
mutable notes: BFP+His
mutable provenance: The BFP is BBa_K592100
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: 6x His
sequence annotation: BBa_K592100
sequence: https://synbiohub.org/public/igem/BBa_K1491001_sequence/1

6x His
type: SequenceAnnotation
persistent identity: annotation2380081
display id: annotation2380081
version: 1
top level: BBa_K1491001
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1491001/annotation2380081/range2380081/1

https://synbiohub.org/public/igem/BBa_K1491001/annotation2380081/range2380081/1
type: Range
persistent identity: range2380081
display id: range2380081
version: 1
direction: direction/forward
top level: BBa_K1491001
start: 700
end: 717
orientation: inline

BBa_K592100
type: SequenceAnnotation
persistent identity: annotation2380080
display id: annotation2380080
version: 1
top level: BBa_K1491001
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1491001/annotation2380080/range2380080/1

https://synbiohub.org/public/igem/BBa_K1491001/annotation2380080/range2380080/1
type: Range
persistent identity: range2380080
display id: range2380080
version: 1
direction: direction/forward
top level: BBa_K1491001
start: 1
end: 699
orientation: inline

BBa_K1833000
type: ComponentDefinition
persistent identity: BBa K1833000
display id: BBa_K1833000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pT7-eGFP
creator: Konstantin Borisov
created: 2015-09-12T11:00:00Z
modified: 2015-09-18T11:04:36Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2259_
m user id: 27388
owner id: 27388
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This part produces GFP in the presence of T7 RNA polymerase. It contains a T7 promoter, a strong RBS, the coding region for GFP, and a double terminator. The GFP is a mutant known as GFPmut3b, and the original citation is as follows: Cormack, B.P., Valdivia, R.H., and S. Falkow. FACS-optimized mutants of green fluorescent protein (GFP). Gene 173: 33-38 (1996). (http://www.sciencedirect.com/science/article/pii/0378111995006850) It has a maximum excitation at 501 nm and maximum emission at 511 nm. For more information, see part BBa_E0040
mutable notes: The part containing GFP was cut with EcoRI and XbaI, and ligated with the annealed oligos to form the completed part. Note that due to the choice of technique, a Biobrick scar site remains between the promoter and RBS.
mutable provenance: The RBS-GFP-terminator was obtained from as part BBa_E0840 from the 2015 Distribution (Plate 2, Well 24D). The T7 promoter was obtained as two short oligonucleotides annealed to form sticky ends. The part containing GFP was then cut with EcoRI and XbaI, and ligated with the annealed oligos to form the completed part. Note that due to the choice of technique, a Biobrick scar site remains between the promoter and RBS.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_E0840
component: BBa_K1833999
sequence annotation: BBa_E0840
sequence annotation: BBa_K1833999
sequence: https://synbiohub.org/public/igem/BBa_K1833000_sequence/1

BBa_E0840
type: Component
persistent identity: component2460130
display id: component2460130
version: 1
top level: BBa_K1833000
access: public
definition: https://synbiohub.org/public/igem/BBa_E0840/1

BBa_K1833999
type: Component
persistent identity: component2460119
display id: component2460119
version: 1
top level: BBa_K1833000
access: public
definition: https://synbiohub.org/public/igem/BBa_K1833999/1

BBa_E0840
type: SequenceAnnotation
persistent identity: annotation2460130
display id: annotation2460130
version: 1
top level: BBa_K1833000
location: https://synbiohub.org/public/igem/BBa_K1833000/annotation2460130/range2460130/1
component: BBa_E0840

https://synbiohub.org/public/igem/BBa_K1833000/annotation2460130/range2460130/1
type: Range
persistent identity: range2460130
display id: range2460130
version: 1
direction: direction/reverse
top level: BBa_K1833000
start: 30
end: 907
orientation: inline

BBa_K1833999
type: SequenceAnnotation
persistent identity: annotation2460119
display id: annotation2460119
version: 1
top level: BBa_K1833000
location: https://synbiohub.org/public/igem/BBa_K1833000/annotation2460119/range2460119/1
component: BBa_K1833999

https://synbiohub.org/public/igem/BBa_K1833000/annotation2460119/range2460119/1
type: Range
persistent identity: range2460119
display id: range2460119
version: 1
direction: direction/reverse
top level: BBa_K1833000
start: 1
end: 29
orientation: inline

CspB | RFP
type: ComponentDefinition
persistent identity: BBa K525234
display id: BBa_K525234
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Fusion Protein of mRFP, S-layer cspB from Corynebacterium halotolerans with TAT-sequence, PT7, RBS
creator: Timo Wolf
created: 2011-09-19T11:00:00Z
modified: 2016-01-25T01:34:53Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _690_
m user id: 4206
owner id: 6668
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Fusion Protein of mRFP and S-layer cspB from Corynebacterium halotolerans with TAT-sequence, PT7 and RBS
mutable notes: Gibson Assembly of BBa_E1010 and BBa_K525224
mutable provenance: Gibson Assembly of BBa_E1010 and BBa_K525224
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: BBa_B0034
sequence annotation: T7 promoter
sequence annotation: BBa_E1010
sequence annotation: RBS
sequence annotation: TAT-sequence
sequence annotation: K525222
sequence annotation: cspB domain
sequence annotation: Linker
sequence annotation: TAA
sequence annotation: mRFP
sequence: https://synbiohub.org/public/igem/BBa_K525234_sequence/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2161284
display id: annotation2161284
version: 1
top level: CspB | RFP
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K525234/annotation2161284/range2161284/1

https://synbiohub.org/public/igem/BBa_K525234/annotation2161284/range2161284/1
type: Range
persistent identity: range2161284
display id: range2161284
version: 1
direction: direction/forward
top level: CspB | RFP
start: 21
end: 33
orientation: inline

T7 promoter
type: SequenceAnnotation
persistent identity: annotation2161283
display id: annotation2161283
version: 1
top level: CspB | RFP
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K525234/annotation2161283/range2161283/1

https://synbiohub.org/public/igem/BBa_K525234/annotation2161283/range2161283/1
type: Range
persistent identity: range2161283
display id: range2161283
version: 1
direction: direction/forward
top level: CspB | RFP
start: 1
end: 20
orientation: inline

BBa_E1010
type: SequenceAnnotation
persistent identity: annotation2161288
display id: annotation2161288
version: 1
top level: CspB | RFP
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K525234/annotation2161288/range2161288/1

https://synbiohub.org/public/igem/BBa_K525234/annotation2161288/range2161288/1
type: Range
persistent identity: range2161288
display id: range2161288
version: 1
direction: direction/forward
top level: CspB | RFP
start: 129
end: 799
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2161285
display id: annotation2161285
version: 1
top level: CspB | RFP
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K525234/annotation2161285/range2161285/1

https://synbiohub.org/public/igem/BBa_K525234/annotation2161285/range2161285/1
type: Range
persistent identity: range2161285
display id: range2161285
version: 1
direction: direction/forward
top level: CspB | RFP
start: 25
end: 28
orientation: inline

TAT-sequence
type: SequenceAnnotation
persistent identity: annotation2161286
display id: annotation2161286
version: 1
top level: CspB | RFP
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K525234/annotation2161286/range2161286/1

https://synbiohub.org/public/igem/BBa_K525234/annotation2161286/range2161286/1
type: Range
persistent identity: range2161286
display id: range2161286
version: 1
direction: direction/forward
top level: CspB | RFP
start: 34
end: 128
orientation: inline

K525222
type: SequenceAnnotation
persistent identity: annotation2161282
display id: annotation2161282
version: 1
top level: CspB | RFP
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K525234/annotation2161282/range2161282/1

https://synbiohub.org/public/igem/BBa_K525234/annotation2161282/range2161282/1
type: Range
persistent identity: range2161282
display id: range2161282
version: 1
direction: direction/forward
top level: CspB | RFP
start: 809
end: 2189
orientation: inline

cspB domain
type: SequenceAnnotation
persistent identity: annotation2161290
display id: annotation2161290
version: 1
top level: CspB | RFP
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K525234/annotation2161290/range2161290/1

https://synbiohub.org/public/igem/BBa_K525234/annotation2161290/range2161290/1
type: Range
persistent identity: range2161290
display id: range2161290
version: 1
direction: direction/forward
top level: CspB | RFP
start: 809
end: 2189
orientation: inline

Linker
type: SequenceAnnotation
persistent identity: annotation2161289
display id: annotation2161289
version: 1
top level: CspB | RFP
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K525234/annotation2161289/range2161289/1

https://synbiohub.org/public/igem/BBa_K525234/annotation2161289/range2161289/1
type: Range
persistent identity: range2161289
display id: range2161289
version: 1
direction: direction/forward
top level: CspB | RFP
start: 800
end: 808
orientation: inline

TAA
type: SequenceAnnotation
persistent identity: annotation2161291
display id: annotation2161291
version: 1
top level: CspB | RFP
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K525234/annotation2161291/range2161291/1

https://synbiohub.org/public/igem/BBa_K525234/annotation2161291/range2161291/1
type: Range
persistent identity: range2161291
display id: range2161291
version: 1
direction: direction/forward
top level: CspB | RFP
start: 2187
end: 2189
orientation: inline

mRFP
type: SequenceAnnotation
persistent identity: annotation2161287
display id: annotation2161287
version: 1
top level: CspB | RFP
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K525234/annotation2161287/range2161287/1

https://synbiohub.org/public/igem/BBa_K525234/annotation2161287/range2161287/1
type: Range
persistent identity: range2161287
display id: range2161287
version: 1
direction: direction/forward
top level: CspB | RFP
start: 129
end: 799
orientation: inline

BBa_K2016005
type: ComponentDefinition
persistent identity: BBa K2016005
display id: BBa_K2016005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Superfolder GFP under control of strong constitutive promoter and RyhB siRNA domain
creator: Magdalena Dabrowska
created: 2016-10-06T11:00:00Z
modified: 2016-10-14T09:08:17Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2483_
m user id: 30333
owner id: 31363
owning group id: 9
status: status/Planning
bookmark: true
mutable description: description
mutable notes: Cloned between XbaI and SpeI sites.
mutable provenance: source
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: RyhB binding site
sequence annotation: Strong promoter
sequence annotation: sfGFP
sequence: https://synbiohub.org/public/igem/BBa_K2016005_sequence/1

RyhB binding site
type: SequenceAnnotation
persistent identity: annotation2516678
display id: annotation2516678
version: 1
top level: BBa_K2016005
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K2016005/annotation2516678/range2516678/1

https://synbiohub.org/public/igem/BBa_K2016005/annotation2516678/range2516678/1
type: Range
persistent identity: range2516678
display id: range2516678
version: 1
direction: direction/forward
top level: BBa_K2016005
start: 73
end: 99
orientation: inline

Strong promoter
type: SequenceAnnotation
persistent identity: annotation2516677
display id: annotation2516677
version: 1
top level: BBa_K2016005
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K2016005/annotation2516677/range2516677/1

https://synbiohub.org/public/igem/BBa_K2016005/annotation2516677/range2516677/1
type: Range
persistent identity: range2516677
display id: range2516677
version: 1
direction: direction/forward
top level: BBa_K2016005
start: 1
end: 35
orientation: inline

sfGFP
type: SequenceAnnotation
persistent identity: annotation2516679
display id: annotation2516679
version: 1
top level: BBa_K2016005
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K2016005/annotation2516679/range2516679/1

https://synbiohub.org/public/igem/BBa_K2016005/annotation2516679/range2516679/1
type: Range
persistent identity: range2516679
display id: range2516679
version: 1
direction: direction/forward
top level: BBa_K2016005
start: 94
end: 810
orientation: inline

BBa_K1486044
type: ComponentDefinition
persistent identity: BBa K1486044
display id: BBa_K1486044
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: IFP[1] Mutated
creator: EPFL iGem team 2014
created: 2014-10-04T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This part is the twin of the non-compatible biobrick BBa_K1486008 rendered compatible by mutating the two illegal restriction sites. However, all experiments were run on the aforementioned biobrick.
mutable notes: Site-directed mutagenesis was used to remove the restriction sites in the sequence.
mutable provenance: http://www.addgene.org/browse/article/8177/ which was then mutated by us.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: IFP1_Mut
sequence: https://synbiohub.org/public/igem/BBa_K1486044_sequence/1

IFP1_Mut
type: SequenceAnnotation
persistent identity: annotation2420792
display id: annotation2420792
version: 1
top level: BBa_K1486044
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1486044/annotation2420792/range2420792/1

https://synbiohub.org/public/igem/BBa_K1486044/annotation2420792/range2420792/1
type: Range
persistent identity: range2420792
display id: range2420792
version: 1
direction: direction/forward
top level: BBa_K1486044
start: 1
end: 402
orientation: inline

BBa_K133055
type: ComponentDefinition
persistent identity: BBa K133055
display id: BBa_K133055
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: mCherry
creator: Ana Lasic
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:09:56Z
discontinued: false
dominant: false
experience: experience/None
group u list: _231_
m user id: 0
owner id: 3352
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: mCherry is a red fluorescent protein used for analysis of localisation of protein.
mutable notes: none
mutable provenance: we got it from
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence: https://synbiohub.org/public/igem/BBa_K133055_sequence/1

eyfp
type: ComponentDefinition
persistent identity: BBa I15013
display id: BBa_I15013
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: B0030.EYFP
creator: Jeff Tabor
created: 2005-05-11T11:00:00Z
modified: 2015-08-31T04:07:38Z
discontinued: false
dominant: true
experience: experience/None
group u list: _5_
m user id: 0
owner id: 88
owning group id: 37
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part accepts POPS to produce EYFP under the strong RBS B0030
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_E0030
component: BBa_B0030
sequence annotation: BBa_E0030
sequence annotation: BBa_B0030
sequence: https://synbiohub.org/public/igem/BBa_I15013_sequence/1

BBa_E0030
type: Component
persistent identity: component1486930
display id: component1486930
version: 1
top level: eyfp
access: public
definition: eyfp

BBa_B0030
type: Component
persistent identity: component1486927
display id: component1486927
version: 1
top level: eyfp
access: public
definition: https://synbiohub.org/public/igem/BBa_B0030/1

BBa_E0030
type: SequenceAnnotation
persistent identity: annotation1486930
display id: annotation1486930
version: 1
top level: eyfp
location: https://synbiohub.org/public/igem/BBa_I15013/annotation1486930/range1486930/1
component: BBa_E0030

https://synbiohub.org/public/igem/BBa_I15013/annotation1486930/range1486930/1
type: Range
persistent identity: range1486930
display id: range1486930
version: 1
direction: direction/forward
top level: eyfp
start: 22
end: 744
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation1486927
display id: annotation1486927
version: 1
top level: eyfp
location: https://synbiohub.org/public/igem/BBa_I15013/annotation1486927/range1486927/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_I15013/annotation1486927/range1486927/1
type: Range
persistent identity: range1486927
display id: range1486927
version: 1
direction: direction/forward
top level: eyfp
start: 1
end: 15
orientation: inline

BBa_K1399002
type: ComponentDefinition
persistent identity: BBa K1399002
display id: BBa_K1399002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RFP from Discosoma striata (coral) with LAA-ssrA degradation tag (wt)
creator: Anna Stikane
created: 2014-09-18T11:00:00Z
modified: 2015-05-08T01:10:15Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1777_
m user id: 0
owner id: 22477
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: Mutant RFP from Discosoma striata (coral) (see part BBa_E1010) with added LAA-ssrA degradation tag (part BBa_M0050). The tag increases RFP turn-over rate, thus providing better temporal resolution of red fluorescence. In the same time, maximal fluorescence amplitudes will be lower as newly formed protein is degraded as soon as it is formed. The tag encodes peptide sequence AANDENYALAA and is recognized by ClpA and ClpX unfoldases and ClpX mediator SspB.[1] ClpA and ClpX then form a proteosome-like complex with ClpP protease and the protein is degraded.[1] The final three residues of the tag determines the strength of interaction with ClpX and thus the final protein degradation rate.[2] The LAA tag is E. coli wild type ssrA tag and is reported to lead to very fast protein degradation, degrading GFP with rate -0.018 per minute.[2] However, be aware that exact protein degradation rate depends on multiple factors: ClpXP and ClpAP protease and SspB mediator concentrations; Protein stability; Km of binding to the protease; Temperature [3]. Mutant RFP from Discosoma striata (coral) (see part BBa_E1010) with added LAA-ssrA degradation tag (part BBa_M0050). The tag increases RFP turn-over rate, thus providing better temporal resolution of red fluorescence. In the same time, maximal fluorescence amplitudes will be lower as newly formed protein is degraded as soon as it is formed. The tag encodes peptide sequence AANDENYALAA and is recognized by ClpA and ClpX unfoldases and ClpX mediator SspB.[1] ClpA and ClpX then form a proteosome-like complex with ClpP protease and the protein is degraded.[1] The final three residues of the tag determines the strength of interaction with ClpX and thus the final protein degradation rate.[2] The LAA tag is E. coli wild type ssrA tag and is reported to lead to very fast protein degradation, degrading GFP with rate -0.018 per minute.[2] However, be aware that exact protein degradation rate depends on multiple factors: ClpXP and ClpAP protease a…
mutable notes: The tag was attached to RFP using PCR and MABEL (mutagenesis with blunt-end ligation), thus avoiding introduction of additonal residues and restriction site. Different parts of the tag are recognized by different proteins, for example, the final 3 residues (AAV in this case) are recognised by ClpX, whereas first 4 residues of the tag are required for efficient SspB binding.[1] Thus modifications of these critical residues alter the efficacy with what different proteases bind to it.
mutable provenance: RFP comes from part BBa_E1010, tag sequence was obtained from part BBa_M0050, but same tag was also used in paper by Andersen et al., (1998).[2]
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: tag
sequence annotation: RFP
sequence annotation: stop
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_K1399002_sequence/1

tag
type: SequenceAnnotation
persistent identity: annotation2383900
display id: annotation2383900
version: 1
top level: BBa_K1399002
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1399002/annotation2383900/range2383900/1

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type: Range
persistent identity: range2383900
display id: range2383900
version: 1
direction: direction/forward
top level: BBa_K1399002
start: 676
end: 708
orientation: inline

RFP
type: SequenceAnnotation
persistent identity: annotation2383899
display id: annotation2383899
version: 1
top level: BBa_K1399002
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1399002/annotation2383899/range2383899/1

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type: Range
persistent identity: range2383899
display id: range2383899
version: 1
direction: direction/forward
top level: BBa_K1399002
start: 4
end: 675
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2383902
display id: annotation2383902
version: 1
top level: BBa_K1399002
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1399002/annotation2383902/range2383902/1

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type: Range
persistent identity: range2383902
display id: range2383902
version: 1
direction: direction/forward
top level: BBa_K1399002
start: 712
end: 714
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2383898
display id: annotation2383898
version: 1
top level: BBa_K1399002
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1399002/annotation2383898/range2383898/1

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type: Range
persistent identity: range2383898
display id: range2383898
version: 1
direction: direction/forward
top level: BBa_K1399002
start: 1
end: 3
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2383901
display id: annotation2383901
version: 1
top level: BBa_K1399002
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1399002/annotation2383901/range2383901/1

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type: Range
persistent identity: range2383901
display id: range2383901
version: 1
direction: direction/forward
top level: BBa_K1399002
start: 709
end: 711
orientation: inline

BBa_E2020
type: ComponentDefinition
persistent identity: BBa E2020
display id: BBa_E2020
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: enhanced version of ECFP, yeast-optimized
creator: ryu
created: 2005-01-24T12:00:00Z
modified: 2015-08-31T04:07:26Z
discontinued: false
dominant: false
experience: experience/None
group u list: _8_
m user id: 0
owner id: 230
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: CFP cerulean, yeast codon optimized.
mutable notes: Contains N- and C-terminal linker sequences (with homology to biobrick parts BBa_E2060 (mCherry), BBa_E2050 (mOrange), and BBa_E2030 (Venus YFP) to facilitate color swapping in yeast. Adds N-"MATSG" and "GSGTA"-C. to published amino acid sequence. Inserts a "V" after normal start M. Double TAATAA stop codon. Missing EcoRI, HindIII, NotI, NdeI, XhoI, RsrII, BamHI, NcoI, BglI, SpeI, XbaI, and PstI. Except for 5' and 3' ends no significant sequence identity runs with Venus YFP (BBa_E2030) or to GFP S65T as found in O'Shea deletion strain collection (originally from plasmid pFA6-GFP(S65T)-His3MX6).
mutable provenance: Rizzo MA, et al. (2004) Nat Biot (22):445-9
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: GSGTA linker
sequence annotation: Cerulean CFP
sequence annotation: MATSG linker
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_E2020_sequence/1

GSGTA linker
type: SequenceAnnotation
persistent identity: annotation1431973
display id: annotation1431973
version: 1
top level: BBa_E2020
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_E2020/annotation1431973/range1431973/1

https://synbiohub.org/public/igem/BBa_E2020/annotation1431973/range1431973/1
type: Range
persistent identity: range1431973
display id: range1431973
version: 1
direction: direction/forward
top level: BBa_E2020
start: 733
end: 747
orientation: inline

Cerulean CFP
type: SequenceAnnotation
persistent identity: annotation1431974
display id: annotation1431974
version: 1
top level: BBa_E2020
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_E2020/annotation1431974/range1431974/1

https://synbiohub.org/public/igem/BBa_E2020/annotation1431974/range1431974/1
type: Range
persistent identity: range1431974
display id: range1431974
version: 1
direction: direction/forward
top level: BBa_E2020
start: 1
end: 747
orientation: inline

MATSG linker
type: SequenceAnnotation
persistent identity: annotation1431972
display id: annotation1431972
version: 1
top level: BBa_E2020
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_E2020/annotation1431972/range1431972/1

https://synbiohub.org/public/igem/BBa_E2020/annotation1431972/range1431972/1
type: Range
persistent identity: range1431972
display id: range1431972
version: 1
direction: direction/forward
top level: BBa_E2020
start: 1
end: 15
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2214038
display id: annotation2214038
version: 1
top level: BBa_E2020
role: SO:0000807
role: feature/barcode
location: https://synbiohub.org/public/igem/BBa_E2020/annotation2214038/range2214038/1

https://synbiohub.org/public/igem/BBa_E2020/annotation2214038/range2214038/1
type: Range
persistent identity: range2214038
display id: range2214038
version: 1
direction: direction/forward
top level: BBa_E2020
start: 754
end: 778
orientation: inline

BBa_K1321356
type: ComponentDefinition
persistent identity: BBa K1321356
display id: BBa_K1321356
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: sfGFP fused to CBDclos driven by LacI
creator: Chris N Micklem
created: 2014-10-07T11:00:00Z
modified: 2016-01-26T09:45:38Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1696_
m user id: 4206
owner id: 20830
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: asdf
mutable notes: awef
mutable provenance: asdf
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_J04500
component: BBa_K1319106
component: BBa_K1321341
sequence annotation: BBa_J04500
sequence annotation: BBa_K1321341
sequence annotation: BBa_K1319106
sequence: https://synbiohub.org/public/igem/BBa_K1321356_sequence/1

BBa_J04500
type: Component
persistent identity: component2415481
display id: component2415481
version: 1
top level: BBa_K1321356
access: public
definition: https://synbiohub.org/public/igem/BBa_J04500/1

BBa_K1319106
type: Component
persistent identity: component2415483
display id: component2415483
version: 1
top level: BBa_K1321356
access: public
definition: https://synbiohub.org/public/igem/BBa_K1319106/1

BBa_K1321341
type: Component
persistent identity: component2415489
display id: component2415489
version: 1
top level: BBa_K1321356
access: public
definition: https://synbiohub.org/public/igem/BBa_K1321341/1

BBa_J04500
type: SequenceAnnotation
persistent identity: annotation2415481
display id: annotation2415481
version: 1
top level: BBa_K1321356
location: https://synbiohub.org/public/igem/BBa_K1321356/annotation2415481/range2415481/1
component: BBa_J04500

https://synbiohub.org/public/igem/BBa_K1321356/annotation2415481/range2415481/1
type: Range
persistent identity: range2415481
display id: range2415481
version: 1
direction: direction/reverse
top level: BBa_K1321356
start: 1
end: 220
orientation: inline

BBa_K1321341
type: SequenceAnnotation
persistent identity: annotation2415489
display id: annotation2415489
version: 1
top level: BBa_K1321356
location: https://synbiohub.org/public/igem/BBa_K1321356/annotation2415489/range2415489/1
component: BBa_K1321341

https://synbiohub.org/public/igem/BBa_K1321356/annotation2415489/range2415489/1
type: Range
persistent identity: range2415489
display id: range2415489
version: 1
direction: direction/reverse
top level: BBa_K1321356
start: 236
end: 1252
orientation: inline

BBa_K1319106
type: SequenceAnnotation
persistent identity: annotation2415483
display id: annotation2415483
version: 1
top level: BBa_K1321356
location: https://synbiohub.org/public/igem/BBa_K1321356/annotation2415483/range2415483/1
component: BBa_K1319106

https://synbiohub.org/public/igem/BBa_K1321356/annotation2415483/range2415483/1
type: Range
persistent identity: range2415483
display id: range2415483
version: 1
direction: direction/reverse
top level: BBa_K1321356
start: 221
end: 235
orientation: inline

BBa_K1399022
type: ComponentDefinition
persistent identity: BBa K1399022
display id: BBa_K1399022
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ptet-GFP(DAS+2)
creator: Anna Stikane
created: 2014-09-18T11:00:00Z
modified: 2015-05-08T01:10:16Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1777_
m user id: 0
owner id: 22477
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: Tetracycline/aTc inducible promoter with GFP reporter tagged with NYADAS-ssrA degradation tag followed by terminator. The tagged GFP is actively degraded within cell, thus provides better temporal resolution of green fluorescence and promoter activity.
mutable notes: Part was assembled using BrickClip assembly (BBF RFC104) using other biobrick parts as templates. BrickClip assembly is a special case of more general Paperclip assembly method.[1]
mutable provenance: other parts
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_B0034
component: BBa_K1399007
component: BBa_B0015
component: BBa_R0040
sequence annotation: BBa_K1399007
sequence annotation: BBa_B0015
sequence annotation: BBa_R0040
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K1399022_sequence/1

BBa_B0034
type: Component
persistent identity: component2384285
display id: component2384285
version: 1
top level: BBa_K1399022
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K1399007
type: Component
persistent identity: component2384291
display id: component2384291
version: 1
top level: BBa_K1399022
access: public
definition: BBa_K1399007

BBa_B0015
type: Component
persistent identity: component2384298
display id: component2384298
version: 1
top level: BBa_K1399022
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_R0040
type: Component
persistent identity: component2384279
display id: component2384279
version: 1
top level: BBa_K1399022
access: public
definition: https://synbiohub.org/public/igem/BBa_R0040/1

BBa_K1399007
type: SequenceAnnotation
persistent identity: annotation2384291
display id: annotation2384291
version: 1
top level: BBa_K1399022
location: https://synbiohub.org/public/igem/BBa_K1399022/annotation2384291/range2384291/1
component: BBa_K1399007

https://synbiohub.org/public/igem/BBa_K1399022/annotation2384291/range2384291/1
type: Range
persistent identity: range2384291
display id: range2384291
version: 1
direction: direction/reverse
top level: BBa_K1399022
start: 81
end: 839
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2384298
display id: annotation2384298
version: 1
top level: BBa_K1399022
location: https://synbiohub.org/public/igem/BBa_K1399022/annotation2384298/range2384298/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K1399022/annotation2384298/range2384298/1
type: Range
persistent identity: range2384298
display id: range2384298
version: 1
direction: direction/reverse
top level: BBa_K1399022
start: 848
end: 976
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2384279
display id: annotation2384279
version: 1
top level: BBa_K1399022
location: https://synbiohub.org/public/igem/BBa_K1399022/annotation2384279/range2384279/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_K1399022/annotation2384279/range2384279/1
type: Range
persistent identity: range2384279
display id: range2384279
version: 1
direction: direction/forward
top level: BBa_K1399022
start: 1
end: 54
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2384285
display id: annotation2384285
version: 1
top level: BBa_K1399022
location: https://synbiohub.org/public/igem/BBa_K1399022/annotation2384285/range2384285/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1399022/annotation2384285/range2384285/1
type: Range
persistent identity: range2384285
display id: range2384285
version: 1
direction: direction/reverse
top level: BBa_K1399022
start: 63
end: 74
orientation: inline

BBa_K180008
type: ComponentDefinition
persistent identity: BBa K180008
display id: BBa_K180008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: mCherry (rights owned by Clontech)
creator: Richard Wilson, Stephanie Chambers
created: 2009-10-15T11:00:00Z
modified: 2016-01-26T10:07:05Z
discontinued: false
dominant: false
experience: experience/None
group u list: _278_
m user id: 4206
owner id: 4082
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: mCherry fluorescent protein - Rights owned by Clontech. Sequence is commercially available from Clontech. Sequence obtained through PCR amplification, with BioBrick prefix and suffix added through in-house designed primers.
mutable notes: PstI site within the mCherry sequence - has to be assembled using BioBrick assembly in such an order that any successive digestions will not require PstI, otherwise the mCherry sequence will be cut as well as the site of interest.
mutable provenance: pmCherry vector, Clontech
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: mCherry
sequence: https://synbiohub.org/public/igem/BBa_K180008_sequence/1

mCherry
type: SequenceAnnotation
persistent identity: annotation2046808
display id: annotation2046808
version: 1
top level: BBa_K180008
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K180008/annotation2046808/range2046808/1

https://synbiohub.org/public/igem/BBa_K180008/annotation2046808/range2046808/1
type: Range
persistent identity: range2046808
display id: range2046808
version: 1
direction: direction/forward
top level: BBa_K180008
start: 1
end: 708
orientation: inline

sfCFP
type: ComponentDefinition
persistent identity: BBa K1093002
display id: BBa_K1093002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: sfCFP
creator: Anna Miścicka
created: 2013-09-19T11:00:00Z
modified: 2016-01-26T03:05:06Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1403_
m user id: 4206
owner id: 18431
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: Superfolder Cyan Fluorescent Protein is a derivative of superfolder Green Fluorescent protein. Compared to CFP it displays improved stability and strong cyan fluorescence.
mutable notes: Derivative of sfGFP, made by PCR
mutable provenance: PartsRegistry BBa_I746909, originally come from Aequorea victoria
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: stop
sequence annotation: sfCFP
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_K1093002_sequence/1

stop
type: SequenceAnnotation
persistent identity: annotation2367768
display id: annotation2367768
version: 1
top level: sfCFP
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1093002/annotation2367768/range2367768/1

https://synbiohub.org/public/igem/BBa_K1093002/annotation2367768/range2367768/1
type: Range
persistent identity: range2367768
display id: range2367768
version: 1
direction: direction/forward
top level: sfCFP
start: 715
end: 720
orientation: inline

sfCFP
type: SequenceAnnotation
persistent identity: annotation2367769
display id: annotation2367769
version: 1
top level: sfCFP
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1093002/annotation2367769/range2367769/1

https://synbiohub.org/public/igem/BBa_K1093002/annotation2367769/range2367769/1
type: Range
persistent identity: range2367769
display id: range2367769
version: 1
direction: direction/forward
top level: sfCFP
start: 1
end: 715
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2367767
display id: annotation2367767
version: 1
top level: sfCFP
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1093002/annotation2367767/range2367767/1

https://synbiohub.org/public/igem/BBa_K1093002/annotation2367767/range2367767/1
type: Range
persistent identity: range2367767
display id: range2367767
version: 1
direction: direction/forward
top level: sfCFP
start: 1
end: 3
orientation: inline

GFP-TS
type: ComponentDefinition
persistent identity: BBa K678029
display id: BBa_K678029
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: GFP-TS for Aspergillus nidulans
creator: DTU-Denmark-2
created: 2011-09-10T11:00:00Z
modified: 2015-05-08T01:13:04Z
discontinued: false
dominant: false
experience: experience/None
group u list: _882_
m user id: 0
owner id: 8341
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: GFP for Aspergillus nidulans
mutable notes: Design considerations This part was designed for the Plug'n'Play with DNA assembly standard (RFC 80), therefore this part contains linkers at the 5' and 3' ends as shown on the figure below.
mutable provenance: To be announced
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: Linker 3
sequence annotation: GFP
sequence: https://synbiohub.org/public/igem/BBa_K678029_sequence/1

Linker 3
type: SequenceAnnotation
persistent identity: annotation2127386
display id: annotation2127386
version: 1
top level: GFP-TS
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678029/annotation2127386/range2127386/1

https://synbiohub.org/public/igem/BBa_K678029/annotation2127386/range2127386/1
type: Range
persistent identity: range2127386
display id: range2127386
version: 1
direction: direction/forward
top level: GFP-TS
start: 1
end: 9
orientation: inline

GFP
type: SequenceAnnotation
persistent identity: annotation2127387
display id: annotation2127387
version: 1
top level: GFP-TS
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K678029/annotation2127387/range2127387/1

https://synbiohub.org/public/igem/BBa_K678029/annotation2127387/range2127387/1
type: Range
persistent identity: range2127387
display id: range2127387
version: 1
direction: direction/forward
top level: GFP-TS
start: 10
end: 729
orientation: inline

BBa_K2023013
type: ComponentDefinition
persistent identity: BBa K2023013
display id: BBa_K2023013
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: XylR coding device with mRFP
creator: Celia Chenebault, Camille Soucies, Benjamin Piot
created: 2016-10-13T11:00:00Z
modified: 2016-10-19T05:06:21Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2490_
m user id: 30356
owner id: 30230
owning group id: 9
status: status/Planning
bookmark: false
mutable description: XylR coding device with mRFP
mutable notes: blabla
mutable provenance: IDT
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
component: BBa_B0034
component: BBa_I723018
component: BBa_E1010
component: BBa_K2023005
component: BBa_B0014
sequence annotation: BBa_K2023005
sequence annotation: BBa_E1010
sequence annotation: BBa_B0014
sequence annotation: BBa_B0034
sequence annotation: BBa_I723018
sequence: https://synbiohub.org/public/igem/BBa_K2023013_sequence/1

BBa_B0034
type: Component
persistent identity: component2521396
display id: component2521396
version: 1
top level: BBa_K2023013
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_I723018
type: Component
persistent identity: component2521394
display id: component2521394
version: 1
top level: BBa_K2023013
access: public
definition: https://synbiohub.org/public/igem/BBa_I723018/1

BBa_E1010
type: Component
persistent identity: component2521403
display id: component2521403
version: 1
top level: BBa_K2023013
access: public
definition: mRFP1

BBa_K2023005
type: Component
persistent identity: component2521398
display id: component2521398
version: 1
top level: BBa_K2023013
access: public
definition: https://synbiohub.org/public/igem/BBa_K2023005/1

BBa_B0014
type: Component
persistent identity: component2521410
display id: component2521410
version: 1
top level: BBa_K2023013
access: public
definition: https://synbiohub.org/public/igem/BBa_B0014/1

BBa_B0034
type: Component
persistent identity: component2521400
display id: component2521400
version: 1
top level: BBa_K2023013
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K2023005
type: SequenceAnnotation
persistent identity: annotation2521398
display id: annotation2521398
version: 1
top level: BBa_K2023013
location: https://synbiohub.org/public/igem/BBa_K2023013/annotation2521398/range2521398/1
component: BBa_K2023005

https://synbiohub.org/public/igem/BBa_K2023013/annotation2521398/range2521398/1
type: Range
persistent identity: range2521398
display id: range2521398
version: 1
direction: direction/reverse
top level: BBa_K2023013
start: 437
end: 2140
orientation: inline

BBa_E1010
type: SequenceAnnotation
persistent identity: annotation2521403
display id: annotation2521403
version: 1
top level: BBa_K2023013
location: https://synbiohub.org/public/igem/BBa_K2023013/annotation2521403/range2521403/1
component: BBa_E1010

https://synbiohub.org/public/igem/BBa_K2023013/annotation2521403/range2521403/1
type: Range
persistent identity: range2521403
display id: range2521403
version: 1
direction: direction/reverse
top level: BBa_K2023013
start: 2167
end: 2872
orientation: inline

BBa_B0014
type: SequenceAnnotation
persistent identity: annotation2521410
display id: annotation2521410
version: 1
top level: BBa_K2023013
location: https://synbiohub.org/public/igem/BBa_K2023013/annotation2521410/range2521410/1
component: BBa_B0014

https://synbiohub.org/public/igem/BBa_K2023013/annotation2521410/range2521410/1
type: Range
persistent identity: range2521410
display id: range2521410
version: 1
direction: direction/reverse
top level: BBa_K2023013
start: 2881
end: 2975
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2521400
display id: annotation2521400
version: 1
top level: BBa_K2023013
location: https://synbiohub.org/public/igem/BBa_K2023013/annotation2521400/range2521400/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K2023013/annotation2521400/range2521400/1
type: Range
persistent identity: range2521400
display id: range2521400
version: 1
direction: direction/reverse
top level: BBa_K2023013
start: 2149
end: 2160
orientation: inline

BBa_I723018
type: SequenceAnnotation
persistent identity: annotation2521394
display id: annotation2521394
version: 1
top level: BBa_K2023013
location: https://synbiohub.org/public/igem/BBa_K2023013/annotation2521394/range2521394/1
component: BBa_I723018

https://synbiohub.org/public/igem/BBa_K2023013/annotation2521394/range2521394/1
type: Range
persistent identity: range2521394
display id: range2521394
version: 1
direction: direction/reverse
top level: BBa_K2023013
start: 1
end: 410
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2521396
display id: annotation2521396
version: 1
top level: BBa_K2023013
location: https://synbiohub.org/public/igem/BBa_K2023013/annotation2521396/range2521396/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K2023013/annotation2521396/range2521396/1
type: Range
persistent identity: range2521396
display id: range2521396
version: 1
direction: direction/reverse
top level: BBa_K2023013
start: 419
end: 430
orientation: inline

BBa_K1673104
type: ComponentDefinition
persistent identity: BBa K1673104
display id: BBa_K1673104
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Oxidation-Resistant RFP
creator: Jarrod Shilts
created: 2015-09-16T11:00:00Z
modified: 2016-01-21T04:03:04Z
discontinued: false
dominant: false
group u list: _2091_
m user id: 4206
owner id: 19415
owning group id: 9
status: status/Planning
bookmark: false
mutable description: RFP sequence, based on E1010, with the number of oxidation-prone sites reduced to the minimum possible amount.
mutable notes: Sequence produced by the Vanderbilt iGEM 2015 mutation optimization software.
mutable provenance: Synthesized DNA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1673104_sequence/1

BBa_K1486014
type: ComponentDefinition
persistent identity: BBa K1486014
display id: BBa_K1486014
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: IFP[1]-CpxR under pBAD
creator: iGem EPFL 2014
created: 2014-08-31T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part has a fused protein downstream of an arabinose-inducible promoter: IFP[1]-CpxR. Biobrick BBa_K1486015 has IFP[2]-CpxR with an arabinose promoter. This construct was made in both orientations: BBa_K1486012 is CpxR-IFP[1] (with an arabinose promoter).
mutable notes: Both orientations were made, as well as for IFP[2] so all dimerizations could be tested (see main page for biobrick numbers). Flexible linkers were used to fuse both proteins, and an RBS was put between the promoter and the cds.
mutable provenance: to edit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_I0500
component: BBa_K1486006
component: BBa_K1486000
component: BBa_B0034
component: BBa_K1486004
sequence annotation: BBa_K1486006
sequence annotation: BBa_K1486004
sequence annotation: BBa_I0500
sequence annotation: BBa_B0034
sequence annotation: BBa_K1486000
sequence: https://synbiohub.org/public/igem/BBa_K1486014_sequence/1

BBa_I0500
type: Component
persistent identity: component2424736
display id: component2424736
version: 1
top level: BBa_K1486014
access: public
definition: https://synbiohub.org/public/igem/BBa_I0500/1

BBa_K1486006
type: Component
persistent identity: component2424742
display id: component2424742
version: 1
top level: BBa_K1486014
access: public
definition: BBa_K1486006

BBa_K1486000
type: Component
persistent identity: component2424747
display id: component2424747
version: 1
top level: BBa_K1486014
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486000/1

BBa_B0034
type: Component
persistent identity: component2424738
display id: component2424738
version: 1
top level: BBa_K1486014
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K1486004
type: Component
persistent identity: component2424743
display id: component2424743
version: 1
top level: BBa_K1486014
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486004/1

BBa_K1486006
type: SequenceAnnotation
persistent identity: annotation2424742
display id: annotation2424742
version: 1
top level: BBa_K1486014
location: https://synbiohub.org/public/igem/BBa_K1486014/annotation2424742/range2424742/1
component: BBa_K1486006

https://synbiohub.org/public/igem/BBa_K1486014/annotation2424742/range2424742/1
type: Range
persistent identity: range2424742
display id: range2424742
version: 1
direction: direction/reverse
top level: BBa_K1486014
start: 1223
end: 1627
orientation: inline

BBa_K1486004
type: SequenceAnnotation
persistent identity: annotation2424743
display id: annotation2424743
version: 1
top level: BBa_K1486014
location: https://synbiohub.org/public/igem/BBa_K1486014/annotation2424743/range2424743/1
component: BBa_K1486004

https://synbiohub.org/public/igem/BBa_K1486014/annotation2424743/range2424743/1
type: Range
persistent identity: range2424743
display id: range2424743
version: 1
direction: direction/reverse
top level: BBa_K1486014
start: 1628
end: 1657
orientation: inline

BBa_I0500
type: SequenceAnnotation
persistent identity: annotation2424736
display id: annotation2424736
version: 1
top level: BBa_K1486014
location: https://synbiohub.org/public/igem/BBa_K1486014/annotation2424736/range2424736/1
component: BBa_I0500

https://synbiohub.org/public/igem/BBa_K1486014/annotation2424736/range2424736/1
type: Range
persistent identity: range2424736
display id: range2424736
version: 1
direction: direction/reverse
top level: BBa_K1486014
start: 1
end: 1210
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2424738
display id: annotation2424738
version: 1
top level: BBa_K1486014
location: https://synbiohub.org/public/igem/BBa_K1486014/annotation2424738/range2424738/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1486014/annotation2424738/range2424738/1
type: Range
persistent identity: range2424738
display id: range2424738
version: 1
direction: direction/reverse
top level: BBa_K1486014
start: 1211
end: 1222
orientation: inline

BBa_K1486000
type: SequenceAnnotation
persistent identity: annotation2424747
display id: annotation2424747
version: 1
top level: BBa_K1486014
location: https://synbiohub.org/public/igem/BBa_K1486014/annotation2424747/range2424747/1
component: BBa_K1486000

https://synbiohub.org/public/igem/BBa_K1486014/annotation2424747/range2424747/1
type: Range
persistent identity: range2424747
display id: range2424747
version: 1
direction: direction/reverse
top level: BBa_K1486014
start: 1658
end: 2359
orientation: inline

GFP-Si4Tag
type: ComponentDefinition
persistent identity: BBa K1602024
display id: BBa_K1602024
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: HisTag-GFP_Si4-Tag fusion protein
creator: Jasmin Weber, Michelle Zoeller, Marcus Geissler, Stefan Bohn, Simon Linder, Sebastian Barthel
created: 2015-09-15T11:00:00Z
modified: 2016-01-25T01:11:28Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2019_
m user id: 4206
owner id: 22022
owning group id: 9
status: status/Planning
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1602024_sequence/1

BBa_K133066
type: ComponentDefinition
persistent identity: BBa K133066
display id: BBa_K133066
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: GFP
creator: Katja Kolar
created: 2008-10-21T11:00:00Z
modified: 2015-05-08T01:09:56Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _231_
m user id: 0
owner id: 1883
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Green fluorescence protein (mCerulean) WITHOUT stop codon.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence: https://synbiohub.org/public/igem/BBa_K133066_sequence/1

BBa_K1732021
type: ComponentDefinition
persistent identity: BBa K1732021
display id: BBa_K1732021
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: BFPCO-His
creator: Donna Lee
created: 2015-09-16T11:00:00Z
modified: 2016-01-21T04:17:57Z
discontinued: false
dominant: false
group u list: _2154_
m user id: 4206
owner id: 27195
owning group id: 9
status: status/Planning
bookmark: false
mutable description: mTagBFP (BBa_K1491001) with a 6X-His tag for easier protein purification. The sequence was codon optimized by IDT Sequencing for E.coli.
mutable notes: Sequence was codon optimized by IDT sequencing for E.coli.
mutable provenance: BBa_K1491001
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: BFPCO
sequence annotation: 6X-His
sequence: https://synbiohub.org/public/igem/BBa_K1732021_sequence/1

BFPCO
type: SequenceAnnotation
persistent identity: annotation2467634
display id: annotation2467634
version: 1
top level: BBa_K1732021
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1732021/annotation2467634/range2467634/1

https://synbiohub.org/public/igem/BBa_K1732021/annotation2467634/range2467634/1
type: Range
persistent identity: range2467634
display id: range2467634
version: 1
direction: direction/forward
top level: BBa_K1732021
start: 1
end: 698
orientation: inline

6X-His
type: SequenceAnnotation
persistent identity: annotation2467635
display id: annotation2467635
version: 1
top level: BBa_K1732021
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1732021/annotation2467635/range2467635/1

https://synbiohub.org/public/igem/BBa_K1732021/annotation2467635/range2467635/1
type: Range
persistent identity: range2467635
display id: range2467635
version: 1
direction: direction/forward
top level: BBa_K1732021
start: 699
end: 717
orientation: inline

SgsE | CFP
type: ComponentDefinition
persistent identity: BBa K525306
display id: BBa_K525306
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Fusion Protein of S-Layer SgsE and mCerulean
creator: Timo Wolf
created: 2011-09-09T11:00:00Z
modified: 2016-01-26T09:40:40Z
discontinued: false
dominant: false
experience: experience/None
group u list: _690_
m user id: 4206
owner id: 6668
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: Fusion Protein of S-Layer SgsE and mCerulean
mutable notes: Fusion Protein of BBa_K525303 and BBa_J18930
mutable provenance: Fusion Protein of S-Layer SgsE and mCerulean
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: J18930
sequence annotation: AgeI
sequence annotation: RBS
sequence annotation: B0034
sequence annotation: K525301
sequence annotation: Freiburg Scar
sequence annotation: lac operator
sequence annotation: TAA
sequence annotation: mCerulean
sequence annotation: SgsE domain
sequence annotation: ATG
sequence annotation: NgoMIV
sequence annotation: T7 promoter
sequence: https://synbiohub.org/public/igem/BBa_K525306_sequence/1

J18930
type: SequenceAnnotation
persistent identity: annotation2128602
display id: annotation2128602
version: 1
top level: SgsE | CFP
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K525306/annotation2128602/range2128602/1

https://synbiohub.org/public/igem/BBa_K525306/annotation2128602/range2128602/1
type: Range
persistent identity: range2128602
display id: range2128602
version: 1
direction: direction/forward
top level: SgsE | CFP
start: 2406
end: 3129
orientation: inline

AgeI
type: SequenceAnnotation
persistent identity: annotation2128605
display id: annotation2128605
version: 1
top level: SgsE | CFP
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K525306/annotation2128605/range2128605/1

https://synbiohub.org/public/igem/BBa_K525306/annotation2128605/range2128605/1
type: Range
persistent identity: range2128605
display id: range2128605
version: 1
direction: direction/forward
top level: SgsE | CFP
start: 3121
end: 3126
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2128598
display id: annotation2128598
version: 1
top level: SgsE | CFP
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K525306/annotation2128598/range2128598/1

https://synbiohub.org/public/igem/BBa_K525306/annotation2128598/range2128598/1
type: Range
persistent identity: range2128598
display id: range2128598
version: 1
direction: direction/forward
top level: SgsE | CFP
start: 56
end: 59
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation2128588
display id: annotation2128588
version: 1
top level: SgsE | CFP
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K525306/annotation2128588/range2128588/1

https://synbiohub.org/public/igem/BBa_K525306/annotation2128588/range2128588/1
type: Range
persistent identity: range2128588
display id: range2128588
version: 1
direction: direction/forward
top level: SgsE | CFP
start: 53
end: 64
orientation: inline

K525301
type: SequenceAnnotation
persistent identity: annotation2128599
display id: annotation2128599
version: 1
top level: SgsE | CFP
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K525306/annotation2128599/range2128599/1

https://synbiohub.org/public/igem/BBa_K525306/annotation2128599/range2128599/1
type: Range
persistent identity: range2128599
display id: range2128599
version: 1
direction: direction/forward
top level: SgsE | CFP
start: 73
end: 2400
orientation: inline

Freiburg Scar
type: SequenceAnnotation
persistent identity: annotation2128601
display id: annotation2128601
version: 1
top level: SgsE | CFP
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K525306/annotation2128601/range2128601/1

https://synbiohub.org/public/igem/BBa_K525306/annotation2128601/range2128601/1
type: Range
persistent identity: range2128601
display id: range2128601
version: 1
direction: direction/forward
top level: SgsE | CFP
start: 2401
end: 2406
orientation: inline

lac operator
type: SequenceAnnotation
persistent identity: annotation2128587
display id: annotation2128587
version: 1
top level: SgsE | CFP
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K525306/annotation2128587/range2128587/1

https://synbiohub.org/public/igem/BBa_K525306/annotation2128587/range2128587/1
type: Range
persistent identity: range2128587
display id: range2128587
version: 1
direction: direction/forward
top level: SgsE | CFP
start: 20
end: 44
orientation: inline

TAA
type: SequenceAnnotation
persistent identity: annotation2128607
display id: annotation2128607
version: 1
top level: SgsE | CFP
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K525306/annotation2128607/range2128607/1

https://synbiohub.org/public/igem/BBa_K525306/annotation2128607/range2128607/1
type: Range
persistent identity: range2128607
display id: range2128607
version: 1
direction: direction/forward
top level: SgsE | CFP
start: 3126
end: 3129
orientation: inline

mCerulean
type: SequenceAnnotation
persistent identity: annotation2128603
display id: annotation2128603
version: 1
top level: SgsE | CFP
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K525306/annotation2128603/range2128603/1

https://synbiohub.org/public/igem/BBa_K525306/annotation2128603/range2128603/1
type: Range
persistent identity: range2128603
display id: range2128603
version: 1
direction: direction/forward
top level: SgsE | CFP
start: 2406
end: 3129
orientation: inline

SgsE domain
type: SequenceAnnotation
persistent identity: annotation2128600
display id: annotation2128600
version: 1
top level: SgsE | CFP
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K525306/annotation2128600/range2128600/1

https://synbiohub.org/public/igem/BBa_K525306/annotation2128600/range2128600/1
type: Range
persistent identity: range2128600
display id: range2128600
version: 1
direction: direction/forward
top level: SgsE | CFP
start: 73
end: 2400
orientation: inline

ATG
type: SequenceAnnotation
persistent identity: annotation2128606
display id: annotation2128606
version: 1
top level: SgsE | CFP
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K525306/annotation2128606/range2128606/1

https://synbiohub.org/public/igem/BBa_K525306/annotation2128606/range2128606/1
type: Range
persistent identity: range2128606
display id: range2128606
version: 1
direction: direction/forward
top level: SgsE | CFP
start: 73
end: 75
orientation: inline

NgoMIV
type: SequenceAnnotation
persistent identity: annotation2128604
display id: annotation2128604
version: 1
top level: SgsE | CFP
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K525306/annotation2128604/range2128604/1

https://synbiohub.org/public/igem/BBa_K525306/annotation2128604/range2128604/1
type: Range
persistent identity: range2128604
display id: range2128604
version: 1
direction: direction/forward
top level: SgsE | CFP
start: 76
end: 81
orientation: inline

T7 promoter
type: SequenceAnnotation
persistent identity: annotation2128585
display id: annotation2128585
version: 1
top level: SgsE | CFP
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K525306/annotation2128585/range2128585/1

https://synbiohub.org/public/igem/BBa_K525306/annotation2128585/range2128585/1
type: Range
persistent identity: range2128585
display id: range2128585
version: 1
direction: direction/forward
top level: SgsE | CFP
start: 1
end: 20
orientation: inline

BBa_K180017
type: ComponentDefinition
persistent identity: BBa K180017
display id: BBa_K180017
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Rock-paper-scissors - Scissors primary plasmid
creator: Richard Wilson, Stephanie Chambers
created: 2009-10-20T11:00:00Z
modified: 2015-05-08T01:11:06Z
discontinued: false
dominant: false
experience: experience/None
group u list: _278_
m user id: 0
owner id: 4082
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: Composite part, consisting of BBa_K180018 and BBa_K180019, but with BBa_K180018 reading along the reverse strand, and BBa_K180019 reading along the forward strand. Forms the 'scissors' primary plasmid in Team:Southampton's rock-paper-scissors project design.
mutable notes: BBa_K180018 reading along reverse strand, and BBa_K180019 reading along the forward strand to ensure the constitutive promoter BBa_J23119 (present in BBa_K180018) has no effect over mBanana or LuxI expression in BBa_K180019.
mutable provenance: See relevant biobrick component parts (BBa_K180018 and BBa_K180019).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: conserved
sequence annotation: mBanana
sequence annotation: lacI
sequence annotation: B0034
sequence annotation: K180000
sequence annotation: C0179
sequence annotation: J23119
sequence annotation: K180019
sequence annotation: B0015
sequence annotation: K180009
sequence annotation: lacIq
sequence annotation: -10
sequence annotation: -35
sequence annotation: K180018
sequence annotation: C0161
sequence annotation: lasR
sequence annotation: Ptac
sequence: https://synbiohub.org/public/igem/BBa_K180017_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation2058468
display id: annotation2058468
version: 1
top level: BBa_K180017
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K180017/annotation2058468/range2058468/1

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type: Range
persistent identity: range2058468
display id: range2058468
version: 1
direction: direction/forward
top level: BBa_K180017
start: 2390
end: 2393
orientation: inline

mBanana
type: SequenceAnnotation
persistent identity: annotation2058470
display id: annotation2058470
version: 1
top level: BBa_K180017
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K180017/annotation2058470/range2058470/1

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type: Range
persistent identity: range2058470
display id: range2058470
version: 1
direction: direction/forward
top level: BBa_K180017
start: 2403
end: 3110
orientation: inline

lacI
type: SequenceAnnotation
persistent identity: annotation2058473
display id: annotation2058473
version: 1
top level: BBa_K180017
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K180017/annotation2058473/range2058473/1

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type: Range
persistent identity: range2058473
display id: range2058473
version: 1
direction: direction/forward
top level: BBa_K180017
start: 3137
end: 3721
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation2058471
display id: annotation2058471
version: 1
top level: BBa_K180017
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K180017/annotation2058471/range2058471/1

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type: Range
persistent identity: range2058471
display id: range2058471
version: 1
direction: direction/forward
top level: BBa_K180017
start: 3119
end: 3130
orientation: inline

K180000
type: SequenceAnnotation
persistent identity: annotation2058463
display id: annotation2058463
version: 1
top level: BBa_K180017
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K180017/annotation2058463/range2058463/1

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type: Range
persistent identity: range2058463
display id: range2058463
version: 1
direction: direction/forward
top level: BBa_K180017
start: 930
end: 2376
orientation: inline

C0179
type: SequenceAnnotation
persistent identity: annotation2058456
display id: annotation2058456
version: 1
top level: BBa_K180017
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K180017/annotation2058456/range2058456/1

https://synbiohub.org/public/igem/BBa_K180017/annotation2058456/range2058456/1
type: Range
persistent identity: range2058456
display id: range2058456
version: 1
direction: direction/reverse
top level: BBa_K180017
start: 138
end: 860
orientation: inline

J23119
type: SequenceAnnotation
persistent identity: annotation2058459
display id: annotation2058459
version: 1
top level: BBa_K180017
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K180017/annotation2058459/range2058459/1

https://synbiohub.org/public/igem/BBa_K180017/annotation2058459/range2058459/1
type: Range
persistent identity: range2058459
display id: range2058459
version: 1
direction: direction/reverse
top level: BBa_K180017
start: 887
end: 921
orientation: inline

K180019
type: SequenceAnnotation
persistent identity: annotation2058464
display id: annotation2058464
version: 1
top level: BBa_K180017
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K180017/annotation2058464/range2058464/1

https://synbiohub.org/public/igem/BBa_K180017/annotation2058464/range2058464/1
type: Range
persistent identity: range2058464
display id: range2058464
version: 1
direction: direction/forward
top level: BBa_K180017
start: 930
end: 3858
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation2058457
display id: annotation2058457
version: 1
top level: BBa_K180017
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K180017/annotation2058457/range2058457/1

https://synbiohub.org/public/igem/BBa_K180017/annotation2058457/range2058457/1
type: Range
persistent identity: range2058457
display id: range2058457
version: 1
direction: direction/reverse
top level: BBa_K180017
start: 867
end: 878
orientation: inline

B0015
type: SequenceAnnotation
persistent identity: annotation2058474
display id: annotation2058474
version: 1
top level: BBa_K180017
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K180017/annotation2058474/range2058474/1

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type: Range
persistent identity: range2058474
display id: range2058474
version: 1
direction: direction/forward
top level: BBa_K180017
start: 3730
end: 3858
orientation: inline

K180009
type: SequenceAnnotation
persistent identity: annotation2058469
display id: annotation2058469
version: 1
top level: BBa_K180017
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K180017/annotation2058469/range2058469/1

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type: Range
persistent identity: range2058469
display id: range2058469
version: 1
direction: direction/forward
top level: BBa_K180017
start: 2403
end: 3110
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation2058466
display id: annotation2058466
version: 1
top level: BBa_K180017
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K180017/annotation2058466/range2058466/1

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type: Range
persistent identity: range2058466
display id: range2058466
version: 1
direction: direction/forward
top level: BBa_K180017
start: 2385
end: 2396
orientation: inline

B0015
type: SequenceAnnotation
persistent identity: annotation2058453
display id: annotation2058453
version: 1
top level: BBa_K180017
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K180017/annotation2058453/range2058453/1

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type: Range
persistent identity: range2058453
display id: range2058453
version: 1
direction: direction/reverse
top level: BBa_K180017
start: 1
end: 129
orientation: inline

conserved
type: SequenceAnnotation
persistent identity: annotation2058458
display id: annotation2058458
version: 1
top level: BBa_K180017
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K180017/annotation2058458/range2058458/1

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type: Range
persistent identity: range2058458
display id: range2058458
version: 1
direction: direction/reverse
top level: BBa_K180017
start: 871
end: 874
orientation: inline

lacIq
type: SequenceAnnotation
persistent identity: annotation2058462
display id: annotation2058462
version: 1
top level: BBa_K180017
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K180017/annotation2058462/range2058462/1

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type: Range
persistent identity: range2058462
display id: range2058462
version: 1
direction: direction/forward
top level: BBa_K180017
start: 930
end: 2011
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2058460
display id: annotation2058460
version: 1
top level: BBa_K180017
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K180017/annotation2058460/range2058460/1

https://synbiohub.org/public/igem/BBa_K180017/annotation2058460/range2058460/1
type: Range
persistent identity: range2058460
display id: range2058460
version: 1
direction: direction/reverse
top level: BBa_K180017
start: 893
end: 898
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2058461
display id: annotation2058461
version: 1
top level: BBa_K180017
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K180017/annotation2058461/range2058461/1

https://synbiohub.org/public/igem/BBa_K180017/annotation2058461/range2058461/1
type: Range
persistent identity: range2058461
display id: range2058461
version: 1
direction: direction/reverse
top level: BBa_K180017
start: 916
end: 921
orientation: inline

K180018
type: SequenceAnnotation
persistent identity: annotation2058454
display id: annotation2058454
version: 1
top level: BBa_K180017
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K180017/annotation2058454/range2058454/1

https://synbiohub.org/public/igem/BBa_K180017/annotation2058454/range2058454/1
type: Range
persistent identity: range2058454
display id: range2058454
version: 1
direction: direction/reverse
top level: BBa_K180017
start: 1
end: 921
orientation: inline

C0161
type: SequenceAnnotation
persistent identity: annotation2058472
display id: annotation2058472
version: 1
top level: BBa_K180017
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K180017/annotation2058472/range2058472/1

https://synbiohub.org/public/igem/BBa_K180017/annotation2058472/range2058472/1
type: Range
persistent identity: range2058472
display id: range2058472
version: 1
direction: direction/forward
top level: BBa_K180017
start: 3137
end: 3721
orientation: inline

lasR
type: SequenceAnnotation
persistent identity: annotation2058455
display id: annotation2058455
version: 1
top level: BBa_K180017
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K180017/annotation2058455/range2058455/1

https://synbiohub.org/public/igem/BBa_K180017/annotation2058455/range2058455/1
type: Range
persistent identity: range2058455
display id: range2058455
version: 1
direction: direction/reverse
top level: BBa_K180017
start: 138
end: 860
orientation: inline

conserved
type: SequenceAnnotation
persistent identity: annotation2058467
display id: annotation2058467
version: 1
top level: BBa_K180017
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K180017/annotation2058467/range2058467/1

https://synbiohub.org/public/igem/BBa_K180017/annotation2058467/range2058467/1
type: Range
persistent identity: range2058467
display id: range2058467
version: 1
direction: direction/forward
top level: BBa_K180017
start: 2389
end: 2392
orientation: inline

Ptac
type: SequenceAnnotation
persistent identity: annotation2058465
display id: annotation2058465
version: 1
top level: BBa_K180017
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K180017/annotation2058465/range2058465/1

https://synbiohub.org/public/igem/BBa_K180017/annotation2058465/range2058465/1
type: Range
persistent identity: range2058465
display id: range2058465
version: 1
direction: direction/forward
top level: BBa_K180017
start: 2254
end: 2281
orientation: inline

BBa_K1031421
type: ComponentDefinition
persistent identity: BBa K1031421
display id: BBa_K1031421
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ph-B0032-sfGFP-Terminator (HcaR)
creator: He Shuaixin
created: 2013-09-08T11:00:00Z
modified: 2015-05-08T01:08:47Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1338_
m user id: 0
owner id: 15843
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Ph-32-sfGFP-TT (HcaR)
mutable notes: molecular cloning
mutable provenance: synthesis, lab made
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: Ph (HcaR)
sequence annotation: sfGFP
sequence annotation: B0015
sequence annotation: B0032
sequence: https://synbiohub.org/public/igem/BBa_K1031421_sequence/1

Ph (HcaR)
type: SequenceAnnotation
persistent identity: annotation2362372
display id: annotation2362372
version: 1
top level: BBa_K1031421
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1031421/annotation2362372/range2362372/1

https://synbiohub.org/public/igem/BBa_K1031421/annotation2362372/range2362372/1
type: Range
persistent identity: range2362372
display id: range2362372
version: 1
direction: direction/forward
top level: BBa_K1031421
start: 1
end: 74
orientation: inline

sfGFP
type: SequenceAnnotation
persistent identity: annotation2362385
display id: annotation2362385
version: 1
top level: BBa_K1031421
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1031421/annotation2362385/range2362385/1

https://synbiohub.org/public/igem/BBa_K1031421/annotation2362385/range2362385/1
type: Range
persistent identity: range2362385
display id: range2362385
version: 1
direction: direction/forward
top level: BBa_K1031421
start: 100
end: 822
orientation: inline

B0015
type: SequenceAnnotation
persistent identity: annotation2362386
display id: annotation2362386
version: 1
top level: BBa_K1031421
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1031421/annotation2362386/range2362386/1

https://synbiohub.org/public/igem/BBa_K1031421/annotation2362386/range2362386/1
type: Range
persistent identity: range2362386
display id: range2362386
version: 1
direction: direction/forward
top level: BBa_K1031421
start: 829
end: 957
orientation: inline

B0032
type: SequenceAnnotation
persistent identity: annotation2362373
display id: annotation2362373
version: 1
top level: BBa_K1031421
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1031421/annotation2362373/range2362373/1

https://synbiohub.org/public/igem/BBa_K1031421/annotation2362373/range2362373/1
type: Range
persistent identity: range2362373
display id: range2362373
version: 1
direction: direction/forward
top level: BBa_K1031421
start: 81
end: 93
orientation: inline

T7 auto-am
type: ComponentDefinition
persistent identity: BBa K606036
display id: BBa_K606036
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pT7 SpoVG T7 SpoVG GFP T7terminator.
creator: Axel S??guret
created: 2011-09-17T11:00:00Z
modified: 2015-05-08T01:12:51Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _778_
m user id: 0
owner id: 8931
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: T7 and GFP expression system due to T7 polymerase
mutable notes: biobrick assembly
mutable provenance: B. subtilis
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K606029
component: BBa_K143021
component: BBa_K145001
component: BBa_I719005
sequence annotation: BBa_K145001
sequence annotation: BBa_K606029
sequence annotation: BBa_I719005
sequence annotation: BBa_K143021
sequence: https://synbiohub.org/public/igem/BBa_K606036_sequence/1

BBa_K606029
type: Component
persistent identity: component2224502
display id: component2224502
version: 1
top level: T7 auto-am
access: public
definition: https://synbiohub.org/public/igem/BBa_K606029/1

BBa_K143021
type: Component
persistent identity: component2224482
display id: component2224482
version: 1
top level: T7 auto-am
access: public
definition: https://synbiohub.org/public/igem/BBa_K143021/1

BBa_K145001
type: Component
persistent identity: component2224487
display id: component2224487
version: 1
top level: T7 auto-am
access: public
definition: https://synbiohub.org/public/igem/BBa_K145001/1

BBa_I719005
type: Component
persistent identity: component2224480
display id: component2224480
version: 1
top level: T7 auto-am
access: public
definition: https://synbiohub.org/public/igem/BBa_I719005/1

BBa_K145001
type: SequenceAnnotation
persistent identity: annotation2224487
display id: annotation2224487
version: 1
top level: T7 auto-am
location: https://synbiohub.org/public/igem/BBa_K606036/annotation2224487/range2224487/1
component: BBa_K145001

https://synbiohub.org/public/igem/BBa_K606036/annotation2224487/range2224487/1
type: Range
persistent identity: range2224487
display id: range2224487
version: 1
direction: direction/reverse
top level: T7 auto-am
start: 50
end: 2704
orientation: inline

BBa_K606029
type: SequenceAnnotation
persistent identity: annotation2224502
display id: annotation2224502
version: 1
top level: T7 auto-am
location: https://synbiohub.org/public/igem/BBa_K606036/annotation2224502/range2224502/1
component: BBa_K606029

https://synbiohub.org/public/igem/BBa_K606036/annotation2224502/range2224502/1
type: Range
persistent identity: range2224502
display id: range2224502
version: 1
direction: direction/reverse
top level: T7 auto-am
start: 2713
end: 3748
orientation: inline

BBa_I719005
type: SequenceAnnotation
persistent identity: annotation2224480
display id: annotation2224480
version: 1
top level: T7 auto-am
location: https://synbiohub.org/public/igem/BBa_K606036/annotation2224480/range2224480/1
component: BBa_I719005

https://synbiohub.org/public/igem/BBa_K606036/annotation2224480/range2224480/1
type: Range
persistent identity: range2224480
display id: range2224480
version: 1
direction: direction/reverse
top level: T7 auto-am
start: 1
end: 23
orientation: inline

BBa_K143021
type: SequenceAnnotation
persistent identity: annotation2224482
display id: annotation2224482
version: 1
top level: T7 auto-am
location: https://synbiohub.org/public/igem/BBa_K606036/annotation2224482/range2224482/1
component: BBa_K143021

https://synbiohub.org/public/igem/BBa_K606036/annotation2224482/range2224482/1
type: Range
persistent identity: range2224482
display id: range2224482
version: 1
direction: direction/reverse
top level: T7 auto-am
start: 32
end: 43
orientation: inline

BBa_K157006
type: ComponentDefinition
persistent identity: BBa K157006
display id: BBa_K157006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Split-Cerulean-nCFP
creator: iGEM Team Freiburg 2008
created: 2008-10-25T11:00:00Z
modified: 2015-05-08T01:10:54Z
discontinued: false
dominant: false
experience: experience/None
group u list: _232_
m user id: 0
owner id: 1673
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: N-terminal fragment (Amino acids1-155) of the monomeric[5] cyan fluorescent protein variant ???Cerulean???, designed for fusion/BiFC[1,3]. Reassembly with Split-Cerulean-cCFP (Part Bba_K157005) generates the complete, working CFP ???Cerulean???, whereas combination with the C-terminal fragment (Part Bba_K157007) of the monomeric yellow fluorescent Protein ???Venus???[4] (Part Bba_I757008) results in green fluorescence[2]. - [1] Chang-Deng Hu, Yurii Chinenov, Tom K. Kerppola: ???Visualization of Interactions among bZIP and Rel Family Proteins in Living Cells Using Bimolecular Fluorescence Complementation???, Molecular Cell, Vol. 9, 789???798, April, 2002 - [2] Chang Deng Hu, Tom K. Kerppola: ???Simultaneous visualization of multiple protein interactions in living cells using multicolor fluorescence complementation analysis???, Nat Biotechnol. 2003 May; 21(5):539-545 (doi:10. 1038/nbt816) -[3] Tom K. Kerppola: ???Design and implementation of bimolecular fluorescence complementation (BiFC) assays for the visualization of protein interactions in living cells???, Nat Protoc. 2006;1(3):1278-1286 (doi:10.1038/nprot.2006.201) -[4]Nagai, T. et al. ???A variant of yellow fluorescent protein with fast and efficient maturation for cell-biological applications??? J. Biol. Chem. 276, 29188-29194, 2001 -[5]Roger Y. Tsien et al. ???Creating new fluorescent probes for cell biology???, Nature Biotechnology Reviews, Vol. 3, 906-918, 2002
mutable notes: Between BioBrick 1.0 sites this part is flanked 5' by NgoMI(=NgoMIV) and 3' AgeI(=PinAI) to facilitate in frame cloning for protein fusions.
mutable provenance: Gene synthesis by GeneArt, optimized for expression in homo sapiens.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: N-terminal portion of Cerulean CFP
sequence: https://synbiohub.org/public/igem/BBa_K157006_sequence/1

N-terminal portion of Cerulean CFP
type: SequenceAnnotation
persistent identity: annotation2064720
display id: annotation2064720
version: 1
top level: BBa_K157006
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157006/annotation2064720/range2064720/1

https://synbiohub.org/public/igem/BBa_K157006/annotation2064720/range2064720/1
type: Range
persistent identity: range2064720
display id: range2064720
version: 1
direction: direction/forward
top level: BBa_K157006
start: 1
end: 465
orientation: inline

BBa_K1486040
type: ComponentDefinition
persistent identity: BBa K1486040
display id: BBa_K1486040
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: sfGFPN + CpxR
creator: EPFL iGem team 2014
created: 2014-09-26T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: To edit
mutable notes: To edit
mutable provenance: To edit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K1486038
component: BBa_I0500
component: BBa_B0034
component: BBa_K1486000
component: BBa_K1486037
sequence annotation: BBa_K1486000
sequence annotation: BBa_I0500
sequence annotation: BBa_K1486037
sequence annotation: BBa_K1486038
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K1486040_sequence/1

BBa_K1486038
type: Component
persistent identity: component2387188
display id: component2387188
version: 1
top level: BBa_K1486040
access: public
definition: BBa_K1486038

BBa_I0500
type: Component
persistent identity: component2387185
display id: component2387185
version: 1
top level: BBa_K1486040
access: public
definition: https://synbiohub.org/public/igem/BBa_I0500/1

BBa_B0034
type: Component
persistent identity: component2387187
display id: component2387187
version: 1
top level: BBa_K1486040
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K1486000
type: Component
persistent identity: component2387193
display id: component2387193
version: 1
top level: BBa_K1486040
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486000/1

BBa_K1486037
type: Component
persistent identity: component2387189
display id: component2387189
version: 1
top level: BBa_K1486040
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486037/1

BBa_K1486000
type: SequenceAnnotation
persistent identity: annotation2387193
display id: annotation2387193
version: 1
top level: BBa_K1486040
location: https://synbiohub.org/public/igem/BBa_K1486040/annotation2387193/range2387193/1
component: BBa_K1486000

https://synbiohub.org/public/igem/BBa_K1486040/annotation2387193/range2387193/1
type: Range
persistent identity: range2387193
display id: range2387193
version: 1
direction: direction/reverse
top level: BBa_K1486040
start: 1904
end: 2605
orientation: inline

BBa_I0500
type: SequenceAnnotation
persistent identity: annotation2387185
display id: annotation2387185
version: 1
top level: BBa_K1486040
location: https://synbiohub.org/public/igem/BBa_K1486040/annotation2387185/range2387185/1
component: BBa_I0500

https://synbiohub.org/public/igem/BBa_K1486040/annotation2387185/range2387185/1
type: Range
persistent identity: range2387185
display id: range2387185
version: 1
direction: direction/reverse
top level: BBa_K1486040
start: 1
end: 1210
orientation: inline

BBa_K1486037
type: SequenceAnnotation
persistent identity: annotation2387189
display id: annotation2387189
version: 1
top level: BBa_K1486040
location: https://synbiohub.org/public/igem/BBa_K1486040/annotation2387189/range2387189/1
component: BBa_K1486037

https://synbiohub.org/public/igem/BBa_K1486040/annotation2387189/range2387189/1
type: Range
persistent identity: range2387189
display id: range2387189
version: 1
direction: direction/reverse
top level: BBa_K1486040
start: 1865
end: 1903
orientation: inline

BBa_K1486038
type: SequenceAnnotation
persistent identity: annotation2387188
display id: annotation2387188
version: 1
top level: BBa_K1486040
location: https://synbiohub.org/public/igem/BBa_K1486040/annotation2387188/range2387188/1
component: BBa_K1486038

https://synbiohub.org/public/igem/BBa_K1486040/annotation2387188/range2387188/1
type: Range
persistent identity: range2387188
display id: range2387188
version: 1
direction: direction/reverse
top level: BBa_K1486040
start: 1223
end: 1864
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2387187
display id: annotation2387187
version: 1
top level: BBa_K1486040
location: https://synbiohub.org/public/igem/BBa_K1486040/annotation2387187/range2387187/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1486040/annotation2387187/range2387187/1
type: Range
persistent identity: range2387187
display id: range2387187
version: 1
direction: direction/reverse
top level: BBa_K1486040
start: 1211
end: 1222
orientation: inline

ecfp
type: ComponentDefinition
persistent identity: BBa E0021
display id: BBa_E0021
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: ECFP (RBS+ LVA-)
creator: Caitlin Conboy and Jennifer Braff
created: 2004-04-21T11:00:00Z
modified: 2015-08-31T04:07:25Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: ECFP with Clontech RBS, no degradation tail
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
sequence annotation: Clontech RBS
sequence annotation: stop
sequence annotation: ECFP
sequence: https://synbiohub.org/public/igem/BBa_E0021_sequence/1

Clontech RBS
type: SequenceAnnotation
persistent identity: annotation353636
display id: annotation353636
version: 1
top level: ecfp
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_E0021/annotation353636/range353636/1

https://synbiohub.org/public/igem/BBa_E0021/annotation353636/range353636/1
type: Range
persistent identity: range353636
display id: range353636
version: 1
direction: direction/forward
top level: ecfp
start: 1
end: 19
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation353638
display id: annotation353638
version: 1
top level: ecfp
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_E0021/annotation353638/range353638/1

https://synbiohub.org/public/igem/BBa_E0021/annotation353638/range353638/1
type: Range
persistent identity: range353638
display id: range353638
version: 1
direction: direction/forward
top level: ecfp
start: 737
end: 739
orientation: inline

ECFP
type: SequenceAnnotation
persistent identity: annotation353637
display id: annotation353637
version: 1
top level: ecfp
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_E0021/annotation353637/range353637/1

https://synbiohub.org/public/igem/BBa_E0021/annotation353637/range353637/1
type: Range
persistent identity: range353637
display id: range353637
version: 1
direction: direction/forward
top level: ecfp
start: 20
end: 736
orientation: inline

BBa_K1468001
type: ComponentDefinition
persistent identity: BBa K1468001
display id: BBa_K1468001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pJ23104 + gene encoding AsRed2
creator: Pedro Luis Dorado Morales
created: 2014-10-02T11:00:00Z
modified: 2015-05-08T01:10:38Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1847_
m user id: 0
owner id: 11756
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: -
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence: https://synbiohub.org/public/igem/BBa_K1468001_sequence/1

mtGFP
type: ComponentDefinition
persistent identity: BBa K809001
display id: BBa_K809001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Mitochondrial GFP
creator: Qiwu XU
created: 2012-09-19T11:00:00Z
modified: 2015-05-08T01:13:26Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1066_
m user id: 0
owner id: 11943
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: Mitochondrial GFP
mutable notes: ..
mutable provenance: ..
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: mt gfp
sequence: https://synbiohub.org/public/igem/BBa_K809001_sequence/1

mt gfp
type: SequenceAnnotation
persistent identity: annotation2188517
display id: annotation2188517
version: 1
top level: mtGFP
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K809001/annotation2188517/range2188517/1

https://synbiohub.org/public/igem/BBa_K809001/annotation2188517/range2188517/1
type: Range
persistent identity: range2188517
display id: range2188517
version: 1
direction: direction/forward
top level: mtGFP
start: 1
end: 714
orientation: inline

mCherry
type: ComponentDefinition
persistent identity: BBa K165004
display id: BBa_K165004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: mCherry, yeast optimized for fusion
creator: John Szymanski
created: 2008-10-25T11:00:00Z
modified: 2015-05-08T01:10:55Z
discontinued: false
dominant: false
experience: experience/None
group u list: _267_
m user id: 0
owner id: 2512
owning group id: 58
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This is the mCherry fluorescent protein, yeast-codon optimized and biobricked in the silver lab BioFusion standard for use in fusion proteins.
mutable notes: -
mutable provenance: From Caroline Ajo-Franklin
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence: https://synbiohub.org/public/igem/BBa_K165004_sequence/1

BBa_K1072017
type: ComponentDefinition
persistent identity: BBa K1072017
display id: BBa_K1072017
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pGAL1 + Flag + odr-10 + GFP + ADH1Te + pFUS1 + BFP + ADH1Te
creator: Junqi kuang
created: 2013-09-20T11:00:00Z
modified: 2015-05-08T01:09:01Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1381_
m user id: 0
owner id: 16002
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: -
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1072013
component: BBa_K1072020
sequence annotation: BBa_K1072020
sequence annotation: BBa_K1072013
sequence: https://synbiohub.org/public/igem/BBa_K1072017_sequence/1

BBa_K1072013
type: Component
persistent identity: component2359111
display id: component2359111
version: 1
top level: BBa_K1072017
access: public
definition: https://synbiohub.org/public/igem/BBa_K1072013/1

BBa_K1072020
type: Component
persistent identity: component2359118
display id: component2359118
version: 1
top level: BBa_K1072017
access: public
definition: https://synbiohub.org/public/igem/BBa_K1072020/1

BBa_K1072020
type: SequenceAnnotation
persistent identity: annotation2359118
display id: annotation2359118
version: 1
top level: BBa_K1072017
location: https://synbiohub.org/public/igem/BBa_K1072017/annotation2359118/range2359118/1
component: BBa_K1072020

https://synbiohub.org/public/igem/BBa_K1072017/annotation2359118/range2359118/1
type: Range
persistent identity: range2359118
display id: range2359118
version: 1
direction: direction/reverse
top level: BBa_K1072017
start: 2700
end: 4169
orientation: inline

BBa_K1072013
type: SequenceAnnotation
persistent identity: annotation2359111
display id: annotation2359111
version: 1
top level: BBa_K1072017
location: https://synbiohub.org/public/igem/BBa_K1072017/annotation2359111/range2359111/1
component: BBa_K1072013

https://synbiohub.org/public/igem/BBa_K1072017/annotation2359111/range2359111/1
type: Range
persistent identity: range2359111
display id: range2359111
version: 1
direction: direction/reverse
top level: BBa_K1072017
start: 1
end: 2691
orientation: inline

BBa_K180001
type: ComponentDefinition
persistent identity: BBa K180001
display id: BBa_K180001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Green fluorescent protein (+LVA)
creator: Richard Wilson, Stephanie Chambers
created: 2009-10-11T11:00:00Z
modified: 2016-01-25T01:37:25Z
discontinued: false
dominant: false
experience: experience/None
group u list: _278_
m user id: 4206
owner id: 4082
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: Based on the part E0040, but with an LVA degradation tail attached.
mutable notes: LVA degradation tail and prefix/suffix addition by sequential PCR amplifications with specially designed primers.
mutable provenance: Based upon GFP from E0040, with LVA degradation tail attached.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: LVA
sequence annotation: GFPmut3b
sequence annotation: K180001
sequence: https://synbiohub.org/public/igem/BBa_K180001_sequence/1

LVA
type: SequenceAnnotation
persistent identity: annotation2034727
display id: annotation2034727
version: 1
top level: BBa_K180001
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K180001/annotation2034727/range2034727/1

https://synbiohub.org/public/igem/BBa_K180001/annotation2034727/range2034727/1
type: Range
persistent identity: range2034727
display id: range2034727
version: 1
direction: direction/forward
top level: BBa_K180001
start: 721
end: 754
orientation: inline

GFPmut3b
type: SequenceAnnotation
persistent identity: annotation2034726
display id: annotation2034726
version: 1
top level: BBa_K180001
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K180001/annotation2034726/range2034726/1

https://synbiohub.org/public/igem/BBa_K180001/annotation2034726/range2034726/1
type: Range
persistent identity: range2034726
display id: range2034726
version: 1
direction: direction/forward
top level: BBa_K180001
start: 1
end: 720
orientation: inline

K180001
type: SequenceAnnotation
persistent identity: annotation2034728
display id: annotation2034728
version: 1
top level: BBa_K180001
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K180001/annotation2034728/range2034728/1

https://synbiohub.org/public/igem/BBa_K180001/annotation2034728/range2034728/1
type: Range
persistent identity: range2034728
display id: range2034728
version: 1
direction: direction/forward
top level: BBa_K180001
start: 1
end: 754
orientation: inline

EYFP
type: ComponentDefinition
persistent identity: BBa E0036
display id: BBa_E0036
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: enhanced yellow fluorescent protein derived from A. victoria GFP
creator: jcbraff
created: 2004-06-06T11:00:00Z
modified: 2015-08-31T04:07:25Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_1_
m user id: 0
owner id: 61
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence: https://synbiohub.org/public/igem/BBa_E0036_sequence/1

BBa_K157005
type: ComponentDefinition
persistent identity: BBa K157005
display id: BBa_K157005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Split-Cerulean-cCFP
creator: iGEM Team Freiburg 2008
created: 2008-10-25T11:00:00Z
modified: 2015-05-08T01:10:54Z
discontinued: false
dominant: false
experience: experience/None
group u list: _232_
m user id: 0
owner id: 1673
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: C-terminal fragment (Amino acids156-236) of the monomeric[5] cyan fluorescent protein variant ???Cerulean???, designed for fusion/BiFC[1,3]. Reassembly with Split-Cerulean-nCFP (Part Bba_K157006) generates the complete, working CFP ???Cerulean???, whereas combination with the N-terminal fragment (Part Bba_K157008) of the monomeric yellow fluorescent Protein ???Venus???[4] (Part Bba_I757008) results in green fluorescence[2].References see "Part Design".
mutable notes: Between BioBrick 1.0 sites this part is flanked 5' by NgoMI(=NgoMIV) and 3' AgeI(=PinAI) to facilitate in frame cloning for protein fusions.
mutable provenance: Gene synthesis by GeneArt, optimized for expression in homo sapiens.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: C-Terminal portion of cerulean CFP
sequence: https://synbiohub.org/public/igem/BBa_K157005_sequence/1

C-Terminal portion of cerulean CFP
type: SequenceAnnotation
persistent identity: annotation2064705
display id: annotation2064705
version: 1
top level: BBa_K157005
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157005/annotation2064705/range2064705/1

https://synbiohub.org/public/igem/BBa_K157005/annotation2064705/range2064705/1
type: Range
persistent identity: range2064705
display id: range2064705
version: 1
direction: direction/forward
top level: BBa_K157005
start: 1
end: 243
orientation: inline

YFP
type: ComponentDefinition
persistent identity: BBa E0032
display id: BBa_E0032
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: enhanced yellow fluorescent protein derived from A. victoria GFP
creator: June Rhee, Connie Tao, Ty Thomson, Louis Waldman
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:25Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Yellow fluorescent protein (EYFP) reporter coding sequence without the Ribosome Binding Site. Modified with an LVA tail for rapid degradation of the protein and faster fall time for the emission. Annotated mutation cause a Q81L mutation that appears to not be near the active site.
mutable notes: BBa_E0032 yellow fluorescent protein is based on BioBrick part BBa_E0031. It has been modified to include a rapid degradation LVA tail, and includes the BioBrick standard assembly head and tail restriction sites. The RBS has been removed. The stop codon has been changed from TAA to a double stop codon TAATAA.
mutable provenance: Modified from <bb_part>BBa_E0031</bb_part>
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: YFP (LVA)
sequence annotation: SsrA
sequence annotation: 2
sequence annotation: A (Q->L)
sequence annotation: BBa_E0032
sequence: https://synbiohub.org/public/igem/BBa_E0032_sequence/1

YFP (LVA)
type: SequenceAnnotation
persistent identity: annotation2156
display id: annotation2156
version: 1
top level: YFP
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_E0032/annotation2156/range2156/1

https://synbiohub.org/public/igem/BBa_E0032/annotation2156/range2156/1
type: Range
persistent identity: range2156
display id: range2156
version: 1
direction: direction/forward
top level: YFP
start: 1
end: 762
orientation: inline

SsrA
type: SequenceAnnotation
persistent identity: annotation2160
display id: annotation2160
version: 1
top level: YFP
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_E0032/annotation2160/range2160/1

https://synbiohub.org/public/igem/BBa_E0032/annotation2160/range2160/1
type: Range
persistent identity: range2160
display id: range2160
version: 1
direction: direction/forward
top level: YFP
start: 718
end: 756
orientation: inline

2
type: SequenceAnnotation
persistent identity: annotation2159
display id: annotation2159
version: 1
top level: YFP
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_E0032/annotation2159/range2159/1

https://synbiohub.org/public/igem/BBa_E0032/annotation2159/range2159/1
type: Range
persistent identity: range2159
display id: range2159
version: 1
direction: direction/forward
top level: YFP
start: 757
end: 762
orientation: inline

A (Q->L)
type: SequenceAnnotation
persistent identity: annotation2161
display id: annotation2161
version: 1
top level: YFP
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_E0032/annotation2161/range2161/1

https://synbiohub.org/public/igem/BBa_E0032/annotation2161/range2161/1
type: Range
persistent identity: range2161
display id: range2161
version: 1
direction: direction/forward
top level: YFP
start: 242
end: 242
orientation: inline

BBa_E0032
type: SequenceAnnotation
persistent identity: annotation7041
display id: annotation7041
version: 1
top level: YFP
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_E0032/annotation7041/range7041/1

https://synbiohub.org/public/igem/BBa_E0032/annotation7041/range7041/1
type: Range
persistent identity: range7041
display id: range7041
version: 1
direction: direction/forward
top level: YFP
start: 1
end: 762
orientation: inline

BBa_K249017
type: ComponentDefinition
persistent identity: BBa K249017
display id: BBa_K249017
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: N-terminal YFP --> Tet inverter
creator: Roxanne Shank
created: 2009-08-25T11:00:00Z
modified: 2015-05-08T01:11:40Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _342_
m user id: 0
owner id: 3171
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Arabinose repressible Tetracycline inversion of YFP with N-terminal Fusion Arganine Tag for targetting into Lumazine Microcompartment. Has a medium RBS and a double terminator.
mutable notes: Standard
mutable provenance: BioBrick Registry and synthesized Arganine Tag
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_B0034
component: BBa_K249001
component: BBa_B0015
component: BBa_K249008
sequence annotation: BBa_K249001
sequence annotation: BBa_B0015
sequence annotation: BBa_B0034
sequence annotation: BBa_K249008
sequence: https://synbiohub.org/public/igem/BBa_K249017_sequence/1

BBa_B0034
type: Component
persistent identity: component2284749
display id: component2284749
version: 1
top level: BBa_K249017
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K249001
type: Component
persistent identity: component2284747
display id: component2284747
version: 1
top level: BBa_K249017
access: public
definition: https://synbiohub.org/public/igem/BBa_K249001/1

BBa_B0015
type: Component
persistent identity: component2284757
display id: component2284757
version: 1
top level: BBa_K249017
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_K249008
type: Component
persistent identity: component2284750
display id: component2284750
version: 1
top level: BBa_K249017
access: public
definition: https://synbiohub.org/public/igem/BBa_K249008/1

BBa_K249001
type: SequenceAnnotation
persistent identity: annotation2284747
display id: annotation2284747
version: 1
top level: BBa_K249017
location: https://synbiohub.org/public/igem/BBa_K249017/annotation2284747/range2284747/1
component: BBa_K249001

https://synbiohub.org/public/igem/BBa_K249017/annotation2284747/range2284747/1
type: Range
persistent identity: range2284747
display id: range2284747
version: 1
direction: direction/reverse
top level: BBa_K249017
start: 1
end: 1040
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2284757
display id: annotation2284757
version: 1
top level: BBa_K249017
location: https://synbiohub.org/public/igem/BBa_K249017/annotation2284757/range2284757/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K249017/annotation2284757/range2284757/1
type: Range
persistent identity: range2284757
display id: range2284757
version: 1
direction: direction/reverse
top level: BBa_K249017
start: 1837
end: 1965
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2284749
display id: annotation2284749
version: 1
top level: BBa_K249017
location: https://synbiohub.org/public/igem/BBa_K249017/annotation2284749/range2284749/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K249017/annotation2284749/range2284749/1
type: Range
persistent identity: range2284749
display id: range2284749
version: 1
direction: direction/reverse
top level: BBa_K249017
start: 1049
end: 1060
orientation: inline

BBa_K249008
type: SequenceAnnotation
persistent identity: annotation2284750
display id: annotation2284750
version: 1
top level: BBa_K249017
location: https://synbiohub.org/public/igem/BBa_K249017/annotation2284750/range2284750/1
component: BBa_K249008

https://synbiohub.org/public/igem/BBa_K249017/annotation2284750/range2284750/1
type: Range
persistent identity: range2284750
display id: range2284750
version: 1
direction: direction/reverse
top level: BBa_K249017
start: 1067
end: 1828
orientation: inline

EYFP
type: ComponentDefinition
persistent identity: BBa E0034
display id: BBa_E0034
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: enhanced yellow fluorescent protein derived from A. victoria GFP
creator: jcbraff
created: 2004-06-06T11:00:00Z
modified: 2015-08-31T04:07:25Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_1_
m user id: 0
owner id: 61
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: AAV
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_E0034_sequence/1

AAV
type: SequenceAnnotation
persistent identity: annotation1245672
display id: annotation1245672
version: 1
top level: EYFP
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_E0034/annotation1245672/range1245672/1

https://synbiohub.org/public/igem/BBa_E0034/annotation1245672/range1245672/1
type: Range
persistent identity: range1245672
display id: range1245672
version: 1
direction: direction/forward
top level: EYFP
start: 718
end: 756
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1245671
display id: annotation1245671
version: 1
top level: EYFP
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_E0034/annotation1245671/range1245671/1

https://synbiohub.org/public/igem/BBa_E0034/annotation1245671/range1245671/1
type: Range
persistent identity: range1245671
display id: range1245671
version: 1
direction: direction/forward
top level: EYFP
start: 757
end: 762
orientation: inline

BBa_K1333204
type: ComponentDefinition
persistent identity: BBa K1333204
display id: BBa_K1333204
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Or2-RBS-GFP-terminator
creator: Yanwen Xie
created: 2014-09-08T11:00:00Z
modified: 2015-05-08T01:09:58Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1708_
m user id: 0
owner id: 15838
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: Fuse lambda cI with LGF2 mutated from Asp to Ala at site 78. As one of the components in Bacterial Two-hybrid System, it causes a less interaction with GAL11P compared to the nomalized WT.
mutable notes: This is a composite part including the lambda cI protein and a mutant of LGF2.
mutable provenance: Mutated LGF2 at site 78 from Asp to Ala and fused it onto lambda cI.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_I12210
component: BBa_E0240
sequence annotation: BBa_E0240
sequence annotation: BBa_I12210
sequence: https://synbiohub.org/public/igem/BBa_K1333204_sequence/1

BBa_I12210
type: Component
persistent identity: component2385881
display id: component2385881
version: 1
top level: BBa_K1333204
access: public
definition: https://synbiohub.org/public/igem/BBa_I12210/1

BBa_E0240
type: Component
persistent identity: component2385892
display id: component2385892
version: 1
top level: BBa_K1333204
access: public
definition: https://synbiohub.org/public/igem/BBa_E0240/1

BBa_E0240
type: SequenceAnnotation
persistent identity: annotation2385892
display id: annotation2385892
version: 1
top level: BBa_K1333204
location: https://synbiohub.org/public/igem/BBa_K1333204/annotation2385892/range2385892/1
component: BBa_E0240

https://synbiohub.org/public/igem/BBa_K1333204/annotation2385892/range2385892/1
type: Range
persistent identity: range2385892
display id: range2385892
version: 1
direction: direction/reverse
top level: BBa_K1333204
start: 79
end: 954
orientation: inline

BBa_I12210
type: SequenceAnnotation
persistent identity: annotation2385881
display id: annotation2385881
version: 1
top level: BBa_K1333204
location: https://synbiohub.org/public/igem/BBa_K1333204/annotation2385881/range2385881/1
component: BBa_I12210

https://synbiohub.org/public/igem/BBa_K1333204/annotation2385881/range2385881/1
type: Range
persistent identity: range2385881
display id: range2385881
version: 1
direction: direction/reverse
top level: BBa_K1333204
start: 1
end: 70
orientation: inline

BBa_K1732025
type: ComponentDefinition
persistent identity: BBa K1732025
display id: BBa_K1732025
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RFPCO-His
creator: Donna Lee
created: 2015-09-17T11:00:00Z
modified: 2016-01-21T02:30:37Z
discontinued: false
dominant: false
group u list: _2154_
m user id: 4206
owner id: 27195
owning group id: 9
status: status/Planning
bookmark: false
mutable description: RFP (BBa_1491008) with 6X His Tag for easier protein purification. Original sequence was codon optimized for E.coli by IDT Codon optimization tool.
mutable notes: His tag for easier protein purification and codon optimized for E.coli.
mutable provenance: BBa_1491008
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: RFPCO
sequence annotation: 6X-His
sequence: https://synbiohub.org/public/igem/BBa_K1732025_sequence/1

RFPCO
type: SequenceAnnotation
persistent identity: annotation2469438
display id: annotation2469438
version: 1
top level: BBa_K1732025
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1732025/annotation2469438/range2469438/1

https://synbiohub.org/public/igem/BBa_K1732025/annotation2469438/range2469438/1
type: Range
persistent identity: range2469438
display id: range2469438
version: 1
direction: direction/forward
top level: BBa_K1732025
start: 1
end: 671
orientation: inline

6X-His
type: SequenceAnnotation
persistent identity: annotation2469439
display id: annotation2469439
version: 1
top level: BBa_K1732025
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1732025/annotation2469439/range2469439/1

https://synbiohub.org/public/igem/BBa_K1732025/annotation2469439/range2469439/1
type: Range
persistent identity: range2469439
display id: range2469439
version: 1
direction: direction/forward
top level: BBa_K1732025
start: 672
end: 690
orientation: inline

AnRe
type: ComponentDefinition
persistent identity: BBa K772006
display id: BBa_K772006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: GFP - LacZ anchor-inducer protein complex
creator: Mustafa Elitok
created: 2012-09-24T11:00:00Z
modified: 2015-05-08T01:13:15Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1024_
m user id: 0
owner id: 9573
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This part contains a green fluorescent protein (GFP) as a non-reactive chemical compound (anchor) in order to neutralize the active reactive fixed to it, a reporter/inducer device or protein (enzyme etc.) and a TEV protease cleavage site between them to assemble these two proteins. BBa_772001 and BBa_K772002 are assembled to be used as the trigger of detection in the whole system which ends with the intracellular transfection (or activation) of TEV protease. After the activation, it is planned that TEV protease will cleave this anchor-inducer protein complex to release free the inducer/reporter. In conclusion, the inducer would activate a transcription pathway on the targeted inducible gene or the reporter would indicate that there is a change in the whole system.
mutable notes: Note that there is an RBS sequence just before the protein sequence in order to eliminate the need of addition of it.
mutable provenance: Cleavage site, LacZ and GFP sequences are gathered from Registry.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
sequence annotation: GFP
sequence annotation: Double Terminator
sequence annotation: Anchor - Reporter
sequence annotation: RBS
sequence annotation: TEV cleavage site
sequence annotation: LacZ
sequence: https://synbiohub.org/public/igem/BBa_K772006_sequence/1

GFP
type: SequenceAnnotation
persistent identity: annotation2203904
display id: annotation2203904
version: 1
top level: AnRe
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K772006/annotation2203904/range2203904/1

https://synbiohub.org/public/igem/BBa_K772006/annotation2203904/range2203904/1
type: Range
persistent identity: range2203904
display id: range2203904
version: 1
direction: direction/forward
top level: AnRe
start: 21
end: 741
orientation: inline

Double Terminator
type: SequenceAnnotation
persistent identity: annotation2203907
display id: annotation2203907
version: 1
top level: AnRe
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K772006/annotation2203907/range2203907/1

https://synbiohub.org/public/igem/BBa_K772006/annotation2203907/range2203907/1
type: Range
persistent identity: range2203907
display id: range2203907
version: 1
direction: direction/forward
top level: AnRe
start: 1076
end: 1207
orientation: inline

Anchor - Reporter
type: SequenceAnnotation
persistent identity: annotation2203908
display id: annotation2203908
version: 1
top level: AnRe
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K772006/annotation2203908/range2203908/1

https://synbiohub.org/public/igem/BBa_K772006/annotation2203908/range2203908/1
type: Range
persistent identity: range2203908
display id: range2203908
version: 1
direction: direction/forward
top level: AnRe
start: 21
end: 1075
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2203903
display id: annotation2203903
version: 1
top level: AnRe
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K772006/annotation2203903/range2203903/1

https://synbiohub.org/public/igem/BBa_K772006/annotation2203903/range2203903/1
type: Range
persistent identity: range2203903
display id: range2203903
version: 1
direction: direction/forward
top level: AnRe
start: 1
end: 20
orientation: inline

TEV cleavage site
type: SequenceAnnotation
persistent identity: annotation2203905
display id: annotation2203905
version: 1
top level: AnRe
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K772006/annotation2203905/range2203905/1

https://synbiohub.org/public/igem/BBa_K772006/annotation2203905/range2203905/1
type: Range
persistent identity: range2203905
display id: range2203905
version: 1
direction: direction/forward
top level: AnRe
start: 742
end: 831
orientation: inline

LacZ
type: SequenceAnnotation
persistent identity: annotation2203906
display id: annotation2203906
version: 1
top level: AnRe
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K772006/annotation2203906/range2203906/1

https://synbiohub.org/public/igem/BBa_K772006/annotation2203906/range2203906/1
type: Range
persistent identity: range2203906
display id: range2203906
version: 1
direction: direction/forward
top level: AnRe
start: 832
end: 1075
orientation: inline

BBa_K1364016
type: ComponentDefinition
persistent identity: BBa K1364016
display id: BBa_K1364016
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PlepA + RFP
creator: Jourdan Camille
created: 2014-10-02T11:00:00Z
modified: 2015-06-08T09:08:05Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1740_
m user id: 4206
owner id: 21465
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: bla
mutable notes: bla
mutable provenance: bla
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_K823002
component: BBa_K606013
sequence annotation: BBa_K823002
sequence annotation: BBa_K606013
sequence: https://synbiohub.org/public/igem/BBa_K1364016_sequence/1

BBa_K823002
type: Component
persistent identity: component2392938
display id: component2392938
version: 1
top level: BBa_K1364016
access: public
definition: https://synbiohub.org/public/igem/BBa_K823002/1

BBa_K606013
type: Component
persistent identity: component2392952
display id: component2392952
version: 1
top level: BBa_K1364016
access: public
definition: https://synbiohub.org/public/igem/BBa_K606013/1

BBa_K823002
type: SequenceAnnotation
persistent identity: annotation2392938
display id: annotation2392938
version: 1
top level: BBa_K1364016
location: https://synbiohub.org/public/igem/BBa_K1364016/annotation2392938/range2392938/1
component: BBa_K823002

https://synbiohub.org/public/igem/BBa_K1364016/annotation2392938/range2392938/1
type: Range
persistent identity: range2392938
display id: range2392938
version: 1
direction: direction/reverse
top level: BBa_K1364016
start: 1
end: 157
orientation: inline

BBa_K606013
type: SequenceAnnotation
persistent identity: annotation2392952
display id: annotation2392952
version: 1
top level: BBa_K1364016
location: https://synbiohub.org/public/igem/BBa_K1364016/annotation2392952/range2392952/1
component: BBa_K606013

https://synbiohub.org/public/igem/BBa_K1364016/annotation2392952/range2392952/1
type: Range
persistent identity: range2392952
display id: range2392952
version: 1
direction: direction/reverse
top level: BBa_K1364016
start: 166
end: 1026
orientation: inline

NdeI+E0040
type: ComponentDefinition
persistent identity: BBa K1819000
display id: BBa_K1819000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: GFP coding sequence with an N-terminal linker
creator: Lais Ribovski
created: 2015-09-12T11:00:00Z
modified: 2015-09-16T07:11:11Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2244_
m user id: 21190
owner id: 21190
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Designed by Brasil-USP team, BBa_K1819000 is a GFP coding sequence with an N-terminal linker, which is 5???-flanked by NdeI restriction site. It allows to characterize protein expression and secretion, and may serve to other IGEM teams that would like to express a chimera C-terminally attached to GFP, which exhibits bright green fluorescence when exposed to light in the blue to ultraviolet range. Such non-usual restriction site, NdeI restriction site, was placed for cloning purposes that lead to expression of recombinant protein with no additional amino acid residue(s) between its coding sequence and the fused N-terminal tag.
mutable notes: As a design consideration we highlight that we chose to add the NdeI restriction site to a GFP coding sequence to avoid any additional amino acid residue between coding sequence and the fused N-terminal tag.
mutable provenance: GFP coding sequence source is BBa_E0040 (http://parts.igem.org/Part:BBa_E0040)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: NdeI site
sequence annotation: binding
sequence annotation: GFP E0040
sequence: https://synbiohub.org/public/igem/BBa_K1819000_sequence/1

NdeI site
type: SequenceAnnotation
persistent identity: annotation2453668
display id: annotation2453668
version: 1
top level: NdeI+E0040
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1819000/annotation2453668/range2453668/1

https://synbiohub.org/public/igem/BBa_K1819000/annotation2453668/range2453668/1
type: Range
persistent identity: range2453668
display id: range2453668
version: 1
direction: direction/forward
top level: NdeI+E0040
start: 1
end: 6
orientation: inline

binding
type: SequenceAnnotation
persistent identity: annotation2462935
display id: annotation2462935
version: 1
top level: NdeI+E0040
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1819000/annotation2462935/range2462935/1

https://synbiohub.org/public/igem/BBa_K1819000/annotation2462935/range2462935/1
type: Range
persistent identity: range2462935
display id: range2462935
version: 1
direction: direction/forward
top level: NdeI+E0040
start: 7
end: 30
orientation: inline

GFP E0040
type: SequenceAnnotation
persistent identity: annotation2453404
display id: annotation2453404
version: 1
top level: NdeI+E0040
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1819000/annotation2453404/range2453404/1

https://synbiohub.org/public/igem/BBa_K1819000/annotation2453404/range2453404/1
type: Range
persistent identity: range2453404
display id: range2453404
version: 1
direction: direction/forward
top level: NdeI+E0040
start: 31
end: 750
orientation: inline

BBa_K1399011
type: ComponentDefinition
persistent identity: BBa K1399011
display id: BBa_K1399011
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Plac-RFP(LVA)
creator: Anna Stikane
created: 2014-09-18T11:00:00Z
modified: 2015-05-08T01:10:16Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1777_
m user id: 0
owner id: 22477
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: Lactose/IPTG inducible promoter with RBS, RFP reporter tagged with LVA-ssrA degradation tag and terminator. The tagged RFP is actively degraded within cell, thus provides better temporal resolution of red fluorescence and promoter activity. This part can be considered as improved version of BBa_J04451 as it does not contain StuI restriction site coding for additional 'RP' residues between RFP and LVA tag.
mutable notes: Part was assembled using BrickClip assembly (BBF RFC 104) using other biobrick parts as templates. BrickClip assembly is a special case of more general Paperclip assembly method.[1]
mutable provenance: other biobricks
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: T7 TE
sequence annotation: stop
sequence annotation: BBa_B0010
sequence annotation: LacI binding site
sequence annotation: BBa_B0015
sequence annotation: conserved
sequence annotation: end of LacI coding region (inactive)
sequence annotation: BBa_K1399001
sequence annotation: start
sequence annotation: LVA-ssrA tag
sequence annotation: -35
sequence annotation: Barcode of BBa_E1010
sequence annotation: -10
sequence annotation: BBa_B0034
sequence annotation: CAP binding site
sequence annotation: stem_loop
sequence annotation: BBa_R0010
sequence annotation: BBa_B0012
sequence annotation: polya
sequence annotation: BBa_E1010 w/o stop
sequence: https://synbiohub.org/public/igem/BBa_K1399011_sequence/1

T7 TE
type: SequenceAnnotation
persistent identity: annotation2386309
display id: annotation2386309
version: 1
top level: BBa_K1399011
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1399011/annotation2386309/range2386309/1

https://synbiohub.org/public/igem/BBa_K1399011/annotation2386309/range2386309/1
type: Range
persistent identity: range2386309
display id: range2386309
version: 1
direction: direction/forward
top level: BBa_K1399011
start: 1069
end: 1088
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2386303
display id: annotation2386303
version: 1
top level: BBa_K1399011
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1399011/annotation2386303/range2386303/1

https://synbiohub.org/public/igem/BBa_K1399011/annotation2386303/range2386303/1
type: Range
persistent identity: range2386303
display id: range2386303
version: 1
direction: direction/forward
top level: BBa_K1399011
start: 938
end: 940
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2386306
display id: annotation2386306
version: 1
top level: BBa_K1399011
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1399011/annotation2386306/range2386306/1

https://synbiohub.org/public/igem/BBa_K1399011/annotation2386306/range2386306/1
type: Range
persistent identity: range2386306
display id: range2386306
version: 1
direction: direction/forward
top level: BBa_K1399011
start: 974
end: 1053
orientation: inline

LacI binding site
type: SequenceAnnotation
persistent identity: annotation2386294
display id: annotation2386294
version: 1
top level: BBa_K1399011
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1399011/annotation2386294/range2386294/1

https://synbiohub.org/public/igem/BBa_K1399011/annotation2386294/range2386294/1
type: Range
persistent identity: range2386294
display id: range2386294
version: 1
direction: direction/forward
top level: BBa_K1399011
start: 166
end: 200
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2386305
display id: annotation2386305
version: 1
top level: BBa_K1399011
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1399011/annotation2386305/range2386305/1

https://synbiohub.org/public/igem/BBa_K1399011/annotation2386305/range2386305/1
type: Range
persistent identity: range2386305
display id: range2386305
version: 1
direction: direction/reverse
top level: BBa_K1399011
start: 974
end: 1102
orientation: inline

conserved
type: SequenceAnnotation
persistent identity: annotation2386297
display id: annotation2386297
version: 1
top level: BBa_K1399011
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K1399011/annotation2386297/range2386297/1

https://synbiohub.org/public/igem/BBa_K1399011/annotation2386297/range2386297/1
type: Range
persistent identity: range2386297
display id: range2386297
version: 1
direction: direction/forward
top level: BBa_K1399011
start: 213
end: 216
orientation: inline

end of LacI coding region (inactive)
type: SequenceAnnotation
persistent identity: annotation2386290
display id: annotation2386290
version: 1
top level: BBa_K1399011
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1399011/annotation2386290/range2386290/1

https://synbiohub.org/public/igem/BBa_K1399011/annotation2386290/range2386290/1
type: Range
persistent identity: range2386290
display id: range2386290
version: 1
direction: direction/forward
top level: BBa_K1399011
start: 1
end: 88
orientation: inline

BBa_K1399001
type: SequenceAnnotation
persistent identity: annotation2386299
display id: annotation2386299
version: 1
top level: BBa_K1399011
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1399011/annotation2386299/range2386299/1

https://synbiohub.org/public/igem/BBa_K1399011/annotation2386299/range2386299/1
type: Range
persistent identity: range2386299
display id: range2386299
version: 1
direction: direction/reverse
top level: BBa_K1399011
start: 227
end: 940
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2386295
display id: annotation2386295
version: 1
top level: BBa_K1399011
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1399011/annotation2386295/range2386295/1

https://synbiohub.org/public/igem/BBa_K1399011/annotation2386295/range2386295/1
type: Range
persistent identity: range2386295
display id: range2386295
version: 1
direction: direction/forward
top level: BBa_K1399011
start: 173
end: 173
orientation: inline

LVA-ssrA tag
type: SequenceAnnotation
persistent identity: annotation2386301
display id: annotation2386301
version: 1
top level: BBa_K1399011
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1399011/annotation2386301/range2386301/1

https://synbiohub.org/public/igem/BBa_K1399011/annotation2386301/range2386301/1
type: Range
persistent identity: range2386301
display id: range2386301
version: 1
direction: direction/forward
top level: BBa_K1399011
start: 902
end: 934
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2386292
display id: annotation2386292
version: 1
top level: BBa_K1399011
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1399011/annotation2386292/range2386292/1

https://synbiohub.org/public/igem/BBa_K1399011/annotation2386292/range2386292/1
type: Range
persistent identity: range2386292
display id: range2386292
version: 1
direction: direction/forward
top level: BBa_K1399011
start: 137
end: 142
orientation: inline

Barcode of BBa_E1010
type: SequenceAnnotation
persistent identity: annotation2386304
display id: annotation2386304
version: 1
top level: BBa_K1399011
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1399011/annotation2386304/range2386304/1

https://synbiohub.org/public/igem/BBa_K1399011/annotation2386304/range2386304/1
type: Range
persistent identity: range2386304
display id: range2386304
version: 1
direction: direction/forward
top level: BBa_K1399011
start: 941
end: 965
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2386293
display id: annotation2386293
version: 1
top level: BBa_K1399011
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1399011/annotation2386293/range2386293/1

https://synbiohub.org/public/igem/BBa_K1399011/annotation2386293/range2386293/1
type: Range
persistent identity: range2386293
display id: range2386293
version: 1
direction: direction/forward
top level: BBa_K1399011
start: 161
end: 166
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2386296
display id: annotation2386296
version: 1
top level: BBa_K1399011
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1399011/annotation2386296/range2386296/1

https://synbiohub.org/public/igem/BBa_K1399011/annotation2386296/range2386296/1
type: Range
persistent identity: range2386296
display id: range2386296
version: 1
direction: direction/reverse
top level: BBa_K1399011
start: 209
end: 220
orientation: inline

CAP binding site
type: SequenceAnnotation
persistent identity: annotation2386291
display id: annotation2386291
version: 1
top level: BBa_K1399011
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1399011/annotation2386291/range2386291/1

https://synbiohub.org/public/igem/BBa_K1399011/annotation2386291/range2386291/1
type: Range
persistent identity: range2386291
display id: range2386291
version: 1
direction: direction/forward
top level: BBa_K1399011
start: 89
end: 126
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation2386307
display id: annotation2386307
version: 1
top level: BBa_K1399011
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1399011/annotation2386307/range2386307/1

https://synbiohub.org/public/igem/BBa_K1399011/annotation2386307/range2386307/1
type: Range
persistent identity: range2386307
display id: range2386307
version: 1
direction: direction/forward
top level: BBa_K1399011
start: 985
end: 1028
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation2386289
display id: annotation2386289
version: 1
top level: BBa_K1399011
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1399011/annotation2386289/range2386289/1

https://synbiohub.org/public/igem/BBa_K1399011/annotation2386289/range2386289/1
type: Range
persistent identity: range2386289
display id: range2386289
version: 1
direction: direction/forward
top level: BBa_K1399011
start: 1
end: 200
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2386298
display id: annotation2386298
version: 1
top level: BBa_K1399011
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1399011/annotation2386298/range2386298/1

https://synbiohub.org/public/igem/BBa_K1399011/annotation2386298/range2386298/1
type: Range
persistent identity: range2386298
display id: range2386298
version: 1
direction: direction/forward
top level: BBa_K1399011
start: 227
end: 229
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2386308
display id: annotation2386308
version: 1
top level: BBa_K1399011
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1399011/annotation2386308/range2386308/1

https://synbiohub.org/public/igem/BBa_K1399011/annotation2386308/range2386308/1
type: Range
persistent identity: range2386308
display id: range2386308
version: 1
direction: direction/forward
top level: BBa_K1399011
start: 1062
end: 1102
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation2386310
display id: annotation2386310
version: 1
top level: BBa_K1399011
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_K1399011/annotation2386310/range2386310/1

https://synbiohub.org/public/igem/BBa_K1399011/annotation2386310/range2386310/1
type: Range
persistent identity: range2386310
display id: range2386310
version: 1
direction: direction/forward
top level: BBa_K1399011
start: 1089
end: 1102
orientation: inline

BBa_E1010 w/o stop
type: SequenceAnnotation
persistent identity: annotation2386300
display id: annotation2386300
version: 1
top level: BBa_K1399011
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1399011/annotation2386300/range2386300/1

https://synbiohub.org/public/igem/BBa_K1399011/annotation2386300/range2386300/1
type: Range
persistent identity: range2386300
display id: range2386300
version: 1
direction: direction/forward
top level: BBa_K1399011
start: 230
end: 901
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2386311
display id: annotation2386311
version: 1
top level: BBa_K1399011
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1399011/annotation2386311/range2386311/1

https://synbiohub.org/public/igem/BBa_K1399011/annotation2386311/range2386311/1
type: Range
persistent identity: range2386311
display id: range2386311
version: 1
direction: direction/forward
top level: BBa_K1399011
start: 1095
end: 1095
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2386302
display id: annotation2386302
version: 1
top level: BBa_K1399011
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1399011/annotation2386302/range2386302/1

https://synbiohub.org/public/igem/BBa_K1399011/annotation2386302/range2386302/1
type: Range
persistent identity: range2386302
display id: range2386302
version: 1
direction: direction/forward
top level: BBa_K1399011
start: 935
end: 937
orientation: inline

BBa_K1486056
type: ComponentDefinition
persistent identity: BBa K1486056
display id: BBa_K1486056
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CxpR & Split IFP1.4 [Cterm + Cterm][2]
creator: iGem EPFL 2014
created: 2014-10-06T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This part is the twin of the non-compatible biobrick BBa_K1486008 rendered compatible by mutating the two illegal restriction sites. However, all experiments were run on the aforementioned biobrick.
mutable notes: Site-directed mutagenesis was used to remove the restriction sites in the sequence.
mutable provenance: http://www.addgene.org/browse/article/8177/ which was then mutated by us.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_K1486045
component: BBa_K1486000
component: BBa_B0034
component: BBa_K1486044
component: BBa_K1486004
component: BBa_I0500
sequence annotation: BBa_I0500
sequence annotation: BBa_K1486000
sequence annotation: BBa_B0034
sequence annotation: BBa_K1486045
sequence annotation: BBa_K1486004
sequence annotation: BBa_K1486044
sequence: https://synbiohub.org/public/igem/BBa_K1486056_sequence/1

BBa_K1486045
type: Component
persistent identity: component2398615
display id: component2398615
version: 1
top level: BBa_K1486056
access: public
definition: BBa_K1486045

BBa_K1486000
type: Component
persistent identity: component2398605
display id: component2398605
version: 1
top level: BBa_K1486056
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486000/1

BBa_B0034
type: Component
persistent identity: component2398601
display id: component2398601
version: 1
top level: BBa_K1486056
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K1486044
type: Component
persistent identity: component2398607
display id: component2398607
version: 1
top level: BBa_K1486056
access: public
definition: BBa_K1486044

BBa_K1486004
type: Component
persistent identity: component2398614
display id: component2398614
version: 1
top level: BBa_K1486056
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486004/1

BBa_K1486004
type: Component
persistent identity: component2398606
display id: component2398606
version: 1
top level: BBa_K1486056
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486004/1

BBa_B0034
type: Component
persistent identity: component2398609
display id: component2398609
version: 1
top level: BBa_K1486056
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_I0500
type: Component
persistent identity: component2398599
display id: component2398599
version: 1
top level: BBa_K1486056
access: public
definition: https://synbiohub.org/public/igem/BBa_I0500/1

BBa_K1486000
type: Component
persistent identity: component2398613
display id: component2398613
version: 1
top level: BBa_K1486056
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486000/1

BBa_I0500
type: SequenceAnnotation
persistent identity: annotation2398599
display id: annotation2398599
version: 1
top level: BBa_K1486056
location: https://synbiohub.org/public/igem/BBa_K1486056/annotation2398599/range2398599/1
component: BBa_I0500

https://synbiohub.org/public/igem/BBa_K1486056/annotation2398599/range2398599/1
type: Range
persistent identity: range2398599
display id: range2398599
version: 1
direction: direction/reverse
top level: BBa_K1486056
start: 1
end: 1210
orientation: inline

BBa_K1486000
type: SequenceAnnotation
persistent identity: annotation2398613
display id: annotation2398613
version: 1
top level: BBa_K1486056
location: https://synbiohub.org/public/igem/BBa_K1486056/annotation2398613/range2398613/1
component: BBa_K1486000

https://synbiohub.org/public/igem/BBa_K1486056/annotation2398613/range2398613/1
type: Range
persistent identity: range2398613
display id: range2398613
version: 1
direction: direction/reverse
top level: BBa_K1486056
start: 2413
end: 3114
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2398601
display id: annotation2398601
version: 1
top level: BBa_K1486056
location: https://synbiohub.org/public/igem/BBa_K1486056/annotation2398601/range2398601/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1486056/annotation2398601/range2398601/1
type: Range
persistent identity: range2398601
display id: range2398601
version: 1
direction: direction/reverse
top level: BBa_K1486056
start: 1219
end: 1230
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2398609
display id: annotation2398609
version: 1
top level: BBa_K1486056
location: https://synbiohub.org/public/igem/BBa_K1486056/annotation2398609/range2398609/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1486056/annotation2398609/range2398609/1
type: Range
persistent identity: range2398609
display id: range2398609
version: 1
direction: direction/reverse
top level: BBa_K1486056
start: 2395
end: 2406
orientation: inline

BBa_K1486045
type: SequenceAnnotation
persistent identity: annotation2398615
display id: annotation2398615
version: 1
top level: BBa_K1486056
location: https://synbiohub.org/public/igem/BBa_K1486056/annotation2398615/range2398615/1
component: BBa_K1486045

https://synbiohub.org/public/igem/BBa_K1486056/annotation2398615/range2398615/1
type: Range
persistent identity: range2398615
display id: range2398615
version: 1
direction: direction/reverse
top level: BBa_K1486056
start: 3161
end: 3742
orientation: inline

BBa_K1486004
type: SequenceAnnotation
persistent identity: annotation2398606
display id: annotation2398606
version: 1
top level: BBa_K1486056
location: https://synbiohub.org/public/igem/BBa_K1486056/annotation2398606/range2398606/1
component: BBa_K1486004

https://synbiohub.org/public/igem/BBa_K1486056/annotation2398606/range2398606/1
type: Range
persistent identity: range2398606
display id: range2398606
version: 1
direction: direction/reverse
top level: BBa_K1486056
start: 1947
end: 1976
orientation: inline

BBa_K1486044
type: SequenceAnnotation
persistent identity: annotation2398607
display id: annotation2398607
version: 1
top level: BBa_K1486056
location: https://synbiohub.org/public/igem/BBa_K1486056/annotation2398607/range2398607/1
component: BBa_K1486044

https://synbiohub.org/public/igem/BBa_K1486056/annotation2398607/range2398607/1
type: Range
persistent identity: range2398607
display id: range2398607
version: 1
direction: direction/reverse
top level: BBa_K1486056
start: 1985
end: 2386
orientation: inline

BBa_K1486000
type: SequenceAnnotation
persistent identity: annotation2398605
display id: annotation2398605
version: 1
top level: BBa_K1486056
location: https://synbiohub.org/public/igem/BBa_K1486056/annotation2398605/range2398605/1
component: BBa_K1486000

https://synbiohub.org/public/igem/BBa_K1486056/annotation2398605/range2398605/1
type: Range
persistent identity: range2398605
display id: range2398605
version: 1
direction: direction/reverse
top level: BBa_K1486056
start: 1237
end: 1938
orientation: inline

BBa_K1486004
type: SequenceAnnotation
persistent identity: annotation2398614
display id: annotation2398614
version: 1
top level: BBa_K1486056
location: https://synbiohub.org/public/igem/BBa_K1486056/annotation2398614/range2398614/1
component: BBa_K1486004

https://synbiohub.org/public/igem/BBa_K1486056/annotation2398614/range2398614/1
type: Range
persistent identity: range2398614
display id: range2398614
version: 1
direction: direction/reverse
top level: BBa_K1486056
start: 3123
end: 3152
orientation: inline

GFPLVA
type: ComponentDefinition
persistent identity: BBa K411207
display id: BBa_K411207
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Green Fluorescent Protein with LVA tag (GFPLVA)
creator: KO KUEI YUEH
created: 2010-10-21T11:00:00Z
modified: 2016-01-28T12:22:52Z
discontinued: false
dominant: true
experience: experience/None
group u list: _526_
m user id: 4206
owner id: 6665
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: GFP with LVA tag can be degrage by SspB
mutable notes: GFP with LVA-tag can be degrade by speedy degrager and has a short half-life
mutable provenance: Biobrick E0040
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: GFP
sequence annotation: LVA tag
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_K411207_sequence/1

GFP
type: SequenceAnnotation
persistent identity: annotation2092218
display id: annotation2092218
version: 1
top level: GFPLVA
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K411207/annotation2092218/range2092218/1

https://synbiohub.org/public/igem/BBa_K411207/annotation2092218/range2092218/1
type: Range
persistent identity: range2092218
display id: range2092218
version: 1
direction: direction/forward
top level: GFPLVA
start: 1
end: 714
orientation: inline

LVA tag
type: SequenceAnnotation
persistent identity: annotation2092217
display id: annotation2092217
version: 1
top level: GFPLVA
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K411207/annotation2092217/range2092217/1

https://synbiohub.org/public/igem/BBa_K411207/annotation2092217/range2092217/1
type: Range
persistent identity: range2092217
display id: range2092217
version: 1
direction: direction/forward
top level: GFPLVA
start: 715
end: 747
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2101796
display id: annotation2101796
version: 1
top level: GFPLVA
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K411207/annotation2101796/range2101796/1

https://synbiohub.org/public/igem/BBa_K411207/annotation2101796/range2101796/1
type: Range
persistent identity: range2101796
display id: range2101796
version: 1
direction: direction/forward
top level: GFPLVA
start: 748
end: 753
orientation: inline

CBDcex(T7)
type: ComponentDefinition
persistent identity: BBa K863103
display id: BBa_K863103
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Cellulose binding Domain from Cellulomonas Fimi with Reporter GFP
creator: Moritz M??ller
created: 2012-09-19T11:00:00Z
modified: 2015-06-17T01:19:26Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1123_
m user id: 4206
owner id: 13080
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Cellulose binding Domain of C. Fimi Exoglucanase gene with T7, RBS linked with two amino acids (GS) to a GFP (Freiburg-Standard)
mutable notes: CBDcex fused to a his-tagged GFP in a Freiburg-manner
mutable provenance: Cloning-vector and [http://partsregistry.org/Part:BBa_K863121 BBa_K863121]
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: Exoglucanase
sequence annotation: BBa_K863121
sequence annotation: RBS
sequence annotation: BBa_K863101
sequence annotation: GS-Linker
sequence annotation: TAA
sequence annotation: BBa_K863102
sequence annotation: AgeI
sequence annotation: ATG
sequence annotation: B0034
sequence annotation: Freiburg-Scar
sequence annotation: BBa_K525998
sequence annotation: His-Tag
sequence annotation: Cellulose binding domain (CBDcex)
sequence annotation: T7 promoter
sequence annotation: BBa_E0040
sequence: https://synbiohub.org/public/igem/BBa_K863103_sequence/1

Exoglucanase
type: SequenceAnnotation
persistent identity: annotation2188018
display id: annotation2188018
version: 1
top level: CBDcex(T7)
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K863103/annotation2188018/range2188018/1

https://synbiohub.org/public/igem/BBa_K863103/annotation2188018/range2188018/1
type: Range
persistent identity: range2188018
display id: range2188018
version: 1
direction: direction/forward
top level: CBDcex(T7)
start: 38
end: 49
orientation: inline

BBa_K863121
type: SequenceAnnotation
persistent identity: annotation2188025
display id: annotation2188025
version: 1
top level: CBDcex(T7)
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K863103/annotation2188025/range2188025/1

https://synbiohub.org/public/igem/BBa_K863103/annotation2188025/range2188025/1
type: Range
persistent identity: range2188025
display id: range2188025
version: 1
direction: direction/forward
top level: CBDcex(T7)
start: 371
end: 1111
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2188016
display id: annotation2188016
version: 1
top level: CBDcex(T7)
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K863103/annotation2188016/range2188016/1

https://synbiohub.org/public/igem/BBa_K863103/annotation2188016/range2188016/1
type: Range
persistent identity: range2188016
display id: range2188016
version: 1
direction: direction/forward
top level: CBDcex(T7)
start: 24
end: 27
orientation: inline

BBa_K863101
type: SequenceAnnotation
persistent identity: annotation2188019
display id: annotation2188019
version: 1
top level: CBDcex(T7)
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K863103/annotation2188019/range2188019/1

https://synbiohub.org/public/igem/BBa_K863103/annotation2188019/range2188019/1
type: Range
persistent identity: range2188019
display id: range2188019
version: 1
direction: direction/forward
top level: CBDcex(T7)
start: 38
end: 364
orientation: inline

GS-Linker
type: SequenceAnnotation
persistent identity: annotation2188022
display id: annotation2188022
version: 1
top level: CBDcex(T7)
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K863103/annotation2188022/range2188022/1

https://synbiohub.org/public/igem/BBa_K863103/annotation2188022/range2188022/1
type: Range
persistent identity: range2188022
display id: range2188022
version: 1
direction: direction/forward
top level: CBDcex(T7)
start: 359
end: 364
orientation: inline

TAA
type: SequenceAnnotation
persistent identity: annotation2188028
display id: annotation2188028
version: 1
top level: CBDcex(T7)
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K863103/annotation2188028/range2188028/1

https://synbiohub.org/public/igem/BBa_K863103/annotation2188028/range2188028/1
type: Range
persistent identity: range2188028
display id: range2188028
version: 1
direction: direction/forward
top level: CBDcex(T7)
start: 1109
end: 1111
orientation: inline

BBa_K863102
type: SequenceAnnotation
persistent identity: annotation2188014
display id: annotation2188014
version: 1
top level: CBDcex(T7)
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K863103/annotation2188014/range2188014/1

https://synbiohub.org/public/igem/BBa_K863103/annotation2188014/range2188014/1
type: Range
persistent identity: range2188014
display id: range2188014
version: 1
direction: direction/forward
top level: CBDcex(T7)
start: 1
end: 364
orientation: inline

AgeI
type: SequenceAnnotation
persistent identity: annotation2188027
display id: annotation2188027
version: 1
top level: CBDcex(T7)
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K863103/annotation2188027/range2188027/1

https://synbiohub.org/public/igem/BBa_K863103/annotation2188027/range2188027/1
type: Range
persistent identity: range2188027
display id: range2188027
version: 1
direction: direction/forward
top level: CBDcex(T7)
start: 1103
end: 1108
orientation: inline

ATG
type: SequenceAnnotation
persistent identity: annotation2188017
display id: annotation2188017
version: 1
top level: CBDcex(T7)
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K863103/annotation2188017/range2188017/1

https://synbiohub.org/public/igem/BBa_K863103/annotation2188017/range2188017/1
type: Range
persistent identity: range2188017
display id: range2188017
version: 1
direction: direction/forward
top level: CBDcex(T7)
start: 35
end: 37
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation2188015
display id: annotation2188015
version: 1
top level: CBDcex(T7)
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K863103/annotation2188015/range2188015/1

https://synbiohub.org/public/igem/BBa_K863103/annotation2188015/range2188015/1
type: Range
persistent identity: range2188015
display id: range2188015
version: 1
direction: direction/forward
top level: CBDcex(T7)
start: 21
end: 32
orientation: inline

Freiburg-Scar
type: SequenceAnnotation
persistent identity: annotation2188023
display id: annotation2188023
version: 1
top level: CBDcex(T7)
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K863103/annotation2188023/range2188023/1

https://synbiohub.org/public/igem/BBa_K863103/annotation2188023/range2188023/1
type: Range
persistent identity: range2188023
display id: range2188023
version: 1
direction: direction/forward
top level: CBDcex(T7)
start: 365
end: 370
orientation: inline

Exoglucanase
type: SequenceAnnotation
persistent identity: annotation2188021
display id: annotation2188021
version: 1
top level: CBDcex(T7)
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K863103/annotation2188021/range2188021/1

https://synbiohub.org/public/igem/BBa_K863103/annotation2188021/range2188021/1
type: Range
persistent identity: range2188021
display id: range2188021
version: 1
direction: direction/forward
top level: CBDcex(T7)
start: 350
end: 358
orientation: inline

BBa_K525998
type: SequenceAnnotation
persistent identity: annotation2188013
display id: annotation2188013
version: 1
top level: CBDcex(T7)
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K863103/annotation2188013/range2188013/1

https://synbiohub.org/public/igem/BBa_K863103/annotation2188013/range2188013/1
type: Range
persistent identity: range2188013
display id: range2188013
version: 1
direction: direction/forward
top level: CBDcex(T7)
start: 1
end: 32
orientation: inline

His-Tag
type: SequenceAnnotation
persistent identity: annotation2188026
display id: annotation2188026
version: 1
top level: CBDcex(T7)
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K863103/annotation2188026/range2188026/1

https://synbiohub.org/public/igem/BBa_K863103/annotation2188026/range2188026/1
type: Range
persistent identity: range2188026
display id: range2188026
version: 1
direction: direction/forward
top level: CBDcex(T7)
start: 1085
end: 1102
orientation: inline

Cellulose binding domain (CBDcex)
type: SequenceAnnotation
persistent identity: annotation2188020
display id: annotation2188020
version: 1
top level: CBDcex(T7)
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K863103/annotation2188020/range2188020/1

https://synbiohub.org/public/igem/BBa_K863103/annotation2188020/range2188020/1
type: Range
persistent identity: range2188020
display id: range2188020
version: 1
direction: direction/forward
top level: CBDcex(T7)
start: 50
end: 349
orientation: inline

T7 promoter
type: SequenceAnnotation
persistent identity: annotation2188012
display id: annotation2188012
version: 1
top level: CBDcex(T7)
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K863103/annotation2188012/range2188012/1

https://synbiohub.org/public/igem/BBa_K863103/annotation2188012/range2188012/1
type: Range
persistent identity: range2188012
display id: range2188012
version: 1
direction: direction/forward
top level: CBDcex(T7)
start: 1
end: 20
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation2188024
display id: annotation2188024
version: 1
top level: CBDcex(T7)
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K863103/annotation2188024/range2188024/1

https://synbiohub.org/public/igem/BBa_K863103/annotation2188024/range2188024/1
type: Range
persistent identity: range2188024
display id: range2188024
version: 1
direction: direction/forward
top level: CBDcex(T7)
start: 371
end: 1084
orientation: inline

BBa_K134900
type: ComponentDefinition
persistent identity: BBa K134900
display id: BBa_K134900
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: GFP
creator: Ileana de la Fuente Colmenares
created: 2009-10-17T11:00:00Z
modified: 2015-05-08T01:10:01Z
discontinued: false
dominant: false
experience: experience/None
group u list: _260_363_
m user id: 0
owner id: 3667
owning group id: 9
sample status: No part sequence
status: status/Planning
bookmark: false
mutable description: this is the gfp protein
mutable notes: GFPmut3* has a couple of mutations from wtGFP - S2R and S65G and S72A. Emerald and EGFP have S65T and multiple other mutations. The S2R mutation should have no effect.
mutable provenance: 1. Elowitz MB and Leibler S. A synthetic oscillatory network of transcriptional regulators. Nature 2000 Jan 20; 403(6767) 335-8. doi:10.1038/35002125 pmid:10659856. PubMed HubMed [Cormack]
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804

BBa_K1185001
type: ComponentDefinition
persistent identity: BBa K1185001
display id: BBa_K1185001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: HBsu-sfGFP
creator: Vincent Leonardo
created: 2013-08-28T11:00:00Z
modified: 2015-06-08T09:31:00Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1498_
m user id: 4206
owner id: 16794
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This BioBrick codes for a HBsu protein attached to a superfolded green fluorescent protein (sfGFP). HBsu is a non-specific DNA binding protein that binds to DNA as a homodimer. The HBsu is joined to the sfGFP through ten amino acid flexible linker sequence. This allows the observation of ''Bacillus subtilis'' DNA using fluorescence microscopy. The integration strategy that we opted for was to clone in the BioBrick into the Multiple cloning site of pMutin4 backbone. First we attached HindIII restriction sites on 5'end and SacII restriction sites on 3'end of the BioBrick. We then cut the pMutin4 backbone and BioBrick using the previously mentioned restriction enzymes, this allowed us to ligate the plasmid and BioBrick together. We also attached a ~300bp amyE homology region onto the 3'end of the BioBrick after the SacII to allow the single cross over and integration of the whole plasmid into the genome. The pMutin4 plasmid contains a ery+ resistance marker for ''B.subtilis'' and amp+ for ''E.coli'' and also contains lacI, lacZ and Pspac promoter which is an IPTG induced promoter which regulated the transcription of this BioBrick. An alternative method to use this part would be to clone this BioBrick out and use any Assembly protocol to attach a desired promoter, RBS and antibiotic resistance genes.
mutable notes: None
mutable provenance: The source of the HBsu coding sequence was the ''B. subtilis'' strain 168 genome, SwissProtTM accession number: P08821. The sfGFP sequence was sourced from BBa_E0040 and the linker from: BBa_K105012.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: sfGFP_BBa_I746916
sequence annotation: 3-frame stop codon
sequence annotation: hbs
sequence annotation: linker sequence BBa_K105012
sequence annotation: RBS-with post spacer
sequence: https://synbiohub.org/public/igem/BBa_K1185001_sequence/1

sfGFP_BBa_I746916
type: SequenceAnnotation
persistent identity: annotation2333335
display id: annotation2333335
version: 1
top level: BBa_K1185001
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1185001/annotation2333335/range2333335/1

https://synbiohub.org/public/igem/BBa_K1185001/annotation2333335/range2333335/1
type: Range
persistent identity: range2333335
display id: range2333335
version: 1
direction: direction/forward
top level: BBa_K1185001
start: 327
end: 1046
orientation: inline

3-frame stop codon
type: SequenceAnnotation
persistent identity: annotation2333336
display id: annotation2333336
version: 1
top level: BBa_K1185001
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1185001/annotation2333336/range2333336/1

https://synbiohub.org/public/igem/BBa_K1185001/annotation2333336/range2333336/1
type: Range
persistent identity: range2333336
display id: range2333336
version: 1
direction: direction/forward
top level: BBa_K1185001
start: 1047
end: 1054
orientation: inline

hbs
type: SequenceAnnotation
persistent identity: annotation2333332
display id: annotation2333332
version: 1
top level: BBa_K1185001
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1185001/annotation2333332/range2333332/1

https://synbiohub.org/public/igem/BBa_K1185001/annotation2333332/range2333332/1
type: Range
persistent identity: range2333332
display id: range2333332
version: 1
direction: direction/forward
top level: BBa_K1185001
start: 21
end: 296
orientation: inline

linker sequence BBa_K105012
type: SequenceAnnotation
persistent identity: annotation2333334
display id: annotation2333334
version: 1
top level: BBa_K1185001
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1185001/annotation2333334/range2333334/1

https://synbiohub.org/public/igem/BBa_K1185001/annotation2333334/range2333334/1
type: Range
persistent identity: range2333334
display id: range2333334
version: 1
direction: direction/forward
top level: BBa_K1185001
start: 297
end: 326
orientation: inline

RBS-with post spacer
type: SequenceAnnotation
persistent identity: annotation2333331
display id: annotation2333331
version: 1
top level: BBa_K1185001
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1185001/annotation2333331/range2333331/1

https://synbiohub.org/public/igem/BBa_K1185001/annotation2333331/range2333331/1
type: Range
persistent identity: range2333331
display id: range2333331
version: 1
direction: direction/forward
top level: BBa_K1185001
start: 1
end: 20
orientation: inline

AnIn
type: ComponentDefinition
persistent identity: BBa K772007
display id: BBa_K772007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: GFP - LasR anchor-inducer protein complex
creator: Mustafa Elitok
created: 2012-09-25T11:00:00Z
modified: 2015-05-08T01:13:15Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1024_
m user id: 0
owner id: 9573
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This part contains a green fluorescent protein (GFP) as a non-reactive chemical compound (anchor) in order to neutralize the active reactive fixed to it, an inducer regulatory protein (LasR is responsible to induce pLas promoter in the presence of 6HSLO3 which is a chemical compound sythesized by an enzyme that LasI gene codes.) and a TEV protease cleavage site between them to assemble these two proteins. BBa_772001 and BBa_K772002 are assembled to be used as the trigger of detection in the whole system which ends with the intracellular transfection (or activation) of TEV protease. After the activation, it is planned that TEV protease will cleave this anchor-inducer protein complex to release free the reporter. In conclusion, in this system, the inducer would activate a transcription pathway on the targeted inducible gene or the reporter would indicate that there is a change in the whole system.
mutable notes: Cleavage site, LasR and GFP sequences are gathered from Registry.
mutable provenance: Note that there is an RBS sequence just before the protein sequence in order to eliminate the need of addition of it.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
sequence annotation: Anchor - Inducer
sequence annotation: LasR
sequence annotation: Double Terminator
sequence annotation: TEV cleavage site
sequence annotation: RBS
sequence annotation: GFP
sequence: https://synbiohub.org/public/igem/BBa_K772007_sequence/1

Anchor - Inducer
type: SequenceAnnotation
persistent identity: annotation2203902
display id: annotation2203902
version: 1
top level: AnIn
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K772007/annotation2203902/range2203902/1

https://synbiohub.org/public/igem/BBa_K772007/annotation2203902/range2203902/1
type: Range
persistent identity: range2203902
display id: range2203902
version: 1
direction: direction/forward
top level: AnIn
start: 21
end: 1636
orientation: inline

LasR
type: SequenceAnnotation
persistent identity: annotation2203901
display id: annotation2203901
version: 1
top level: AnIn
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K772007/annotation2203901/range2203901/1

https://synbiohub.org/public/igem/BBa_K772007/annotation2203901/range2203901/1
type: Range
persistent identity: range2203901
display id: range2203901
version: 1
direction: direction/forward
top level: AnIn
start: 880
end: 1636
orientation: inline

Double Terminator
type: SequenceAnnotation
persistent identity: annotation2203909
display id: annotation2203909
version: 1
top level: AnIn
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K772007/annotation2203909/range2203909/1

https://synbiohub.org/public/igem/BBa_K772007/annotation2203909/range2203909/1
type: Range
persistent identity: range2203909
display id: range2203909
version: 1
direction: direction/forward
top level: AnIn
start: 1636
end: 1720
orientation: inline

TEV cleavage site
type: SequenceAnnotation
persistent identity: annotation2203900
display id: annotation2203900
version: 1
top level: AnIn
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K772007/annotation2203900/range2203900/1

https://synbiohub.org/public/igem/BBa_K772007/annotation2203900/range2203900/1
type: Range
persistent identity: range2203900
display id: range2203900
version: 1
direction: direction/forward
top level: AnIn
start: 782
end: 871
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2203898
display id: annotation2203898
version: 1
top level: AnIn
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K772007/annotation2203898/range2203898/1

https://synbiohub.org/public/igem/BBa_K772007/annotation2203898/range2203898/1
type: Range
persistent identity: range2203898
display id: range2203898
version: 1
direction: direction/forward
top level: AnIn
start: 1
end: 20
orientation: inline

GFP
type: SequenceAnnotation
persistent identity: annotation2203899
display id: annotation2203899
version: 1
top level: AnIn
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K772007/annotation2203899/range2203899/1

https://synbiohub.org/public/igem/BBa_K772007/annotation2203899/range2203899/1
type: Range
persistent identity: range2203899
display id: range2203899
version: 1
direction: direction/forward
top level: AnIn
start: 21
end: 741
orientation: inline

BBa_K2023016
type: ComponentDefinition
persistent identity: BBa K2023016
display id: BBa_K2023016
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: GFP coding device with Pu
creator: C??lia Chenebault
created: 2016-10-13T11:00:00Z
modified: 2016-10-20T04:05:09Z
discontinued: false
dominant: false
group u list: _2490_
m user id: 30356
owner id: 30230
owning group id: 9
status: status/Planning
bookmark: false
mutable description: GFP coding device with Pu
mutable notes: blabla
mutable provenance: Pu promoter and RBS from GLuc coding device with Pu (BBa_K2023003) and GFP reporter for RHS of library test constructs (BBa_I13401)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_E0040
component: BBa_B0034
component: BBa_B0015
component: BBa_I723020
sequence annotation: BBa_B0015
sequence annotation: BBa_E0040
sequence annotation: BBa_I723020
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K2023016_sequence/1

BBa_E0040
type: Component
persistent identity: component2518998
display id: component2518998
version: 1
top level: BBa_K2023016
access: public
definition: GFP

BBa_B0034
type: Component
persistent identity: component2518996
display id: component2518996
version: 1
top level: BBa_K2023016
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0015
type: Component
persistent identity: component2519005
display id: component2519005
version: 1
top level: BBa_K2023016
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_I723020
type: Component
persistent identity: component2518994
display id: component2518994
version: 1
top level: BBa_K2023016
access: public
definition: https://synbiohub.org/public/igem/BBa_I723020/1

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2519005
display id: annotation2519005
version: 1
top level: BBa_K2023016
location: https://synbiohub.org/public/igem/BBa_K2023016/annotation2519005/range2519005/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K2023016/annotation2519005/range2519005/1
type: Range
persistent identity: range2519005
display id: range2519005
version: 1
direction: direction/reverse
top level: BBa_K2023016
start: 1075
end: 1203
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation2518998
display id: annotation2518998
version: 1
top level: BBa_K2023016
location: https://synbiohub.org/public/igem/BBa_K2023016/annotation2518998/range2518998/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_K2023016/annotation2518998/range2518998/1
type: Range
persistent identity: range2518998
display id: range2518998
version: 1
direction: direction/reverse
top level: BBa_K2023016
start: 347
end: 1066
orientation: inline

BBa_I723020
type: SequenceAnnotation
persistent identity: annotation2518994
display id: annotation2518994
version: 1
top level: BBa_K2023016
location: https://synbiohub.org/public/igem/BBa_K2023016/annotation2518994/range2518994/1
component: BBa_I723020

https://synbiohub.org/public/igem/BBa_K2023016/annotation2518994/range2518994/1
type: Range
persistent identity: range2518994
display id: range2518994
version: 1
direction: direction/reverse
top level: BBa_K2023016
start: 1
end: 320
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2518996
display id: annotation2518996
version: 1
top level: BBa_K2023016
location: https://synbiohub.org/public/igem/BBa_K2023016/annotation2518996/range2518996/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K2023016/annotation2518996/range2518996/1
type: Range
persistent identity: range2518996
display id: range2518996
version: 1
direction: direction/reverse
top level: BBa_K2023016
start: 329
end: 340
orientation: inline

BBa_K819007
type: ComponentDefinition
persistent identity: BBa K819007
display id: BBa_K819007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Measurement device for Luminesensor
creator: YU Zhou
created: 2012-09-02T11:00:00Z
modified: 2015-05-08T01:13:29Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1077_
m user id: 0
owner id: 13785
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: recA408 Promoter + B0030 + GFP + ssrA-tag
mutable notes: recA408 Promoter + B0030 + GFP + ssrA-tag
mutable provenance: From Parts Registry
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: RecA408 Promoter
sequence annotation: RBS-GFP-ssrA:BBa_K581007
sequence: https://synbiohub.org/public/igem/BBa_K819007_sequence/1

RecA408 Promoter
type: SequenceAnnotation
persistent identity: annotation2197244
display id: annotation2197244
version: 1
top level: BBa_K819007
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K819007/annotation2197244/range2197244/1

https://synbiohub.org/public/igem/BBa_K819007/annotation2197244/range2197244/1
type: Range
persistent identity: range2197244
display id: range2197244
version: 1
direction: direction/forward
top level: BBa_K819007
start: 1
end: 79
orientation: inline

RBS-GFP-ssrA:BBa_K581007
type: SequenceAnnotation
persistent identity: annotation2197250
display id: annotation2197250
version: 1
top level: BBa_K819007
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K819007/annotation2197250/range2197250/1

https://synbiohub.org/public/igem/BBa_K819007/annotation2197250/range2197250/1
type: Range
persistent identity: range2197250
display id: range2197250
version: 1
direction: direction/forward
top level: BBa_K819007
start: 80
end: 857
orientation: inline

BBa_K191004
type: ComponentDefinition
persistent identity: BBa K191004
display id: BBa_K191004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TRP promoter - RBS - RFP - Term
creator: Le Thanh Tu NGUYEN
created: 2009-10-10T11:00:00Z
modified: 2015-05-08T01:11:15Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _287_
m user id: 0
owner id: 4198
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: Readout 1, RFP's transcription controlled by TRP operon
mutable notes: TRP operon was added by PCR.
mutable provenance: See subpart's reference
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_I13507
component: BBa_K191007
sequence annotation: BBa_I13507
sequence annotation: BBa_K191007
sequence: https://synbiohub.org/public/igem/BBa_K191004_sequence/1

BBa_I13507
type: Component
persistent identity: component2255985
display id: component2255985
version: 1
top level: BBa_K191004
access: public
definition: https://synbiohub.org/public/igem/BBa_I13507/1

BBa_K191007
type: Component
persistent identity: component2255973
display id: component2255973
version: 1
top level: BBa_K191004
access: public
definition: https://synbiohub.org/public/igem/BBa_K191007/1

BBa_I13507
type: SequenceAnnotation
persistent identity: annotation2255985
display id: annotation2255985
version: 1
top level: BBa_K191004
location: https://synbiohub.org/public/igem/BBa_K191004/annotation2255985/range2255985/1
component: BBa_I13507

https://synbiohub.org/public/igem/BBa_K191004/annotation2255985/range2255985/1
type: Range
persistent identity: range2255985
display id: range2255985
version: 1
direction: direction/reverse
top level: BBa_K191004
start: 94
end: 954
orientation: inline

BBa_K191007
type: SequenceAnnotation
persistent identity: annotation2255973
display id: annotation2255973
version: 1
top level: BBa_K191004
location: https://synbiohub.org/public/igem/BBa_K191004/annotation2255973/range2255973/1
component: BBa_K191007

https://synbiohub.org/public/igem/BBa_K191004/annotation2255973/range2255973/1
type: Range
persistent identity: range2255973
display id: range2255973
version: 1
direction: direction/reverse
top level: BBa_K191004
start: 1
end: 85
orientation: inline

BBa_K256029
type: ComponentDefinition
persistent identity: BBa K256029
display id: BBa_K256029
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: placI:GFP
creator: iGEM09_NTU-Singapore
created: 2009-10-18T11:00:00Z
modified: 2015-05-08T01:11:41Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _359_
m user id: 0
owner id: 4864
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: GFP generator. Inducible using IPTG/Lactose.
mutable notes: --
mutable provenance: --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_R0010
component: BBa_B0010
component: BBa_E0040
component: BBa_B0012
component: BBa_B0034
sequence annotation: BBa_R0010
sequence annotation: BBa_B0034
sequence annotation: BBa_B0010
sequence annotation: BBa_E0040
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_K256029_sequence/1

BBa_R0010
type: Component
persistent identity: component2052776
display id: component2052776
version: 1
top level: BBa_K256029
access: public
definition: https://synbiohub.org/public/igem/BBa_R0010/1

BBa_B0010
type: Component
persistent identity: component2052787
display id: component2052787
version: 1
top level: BBa_K256029
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_E0040
type: Component
persistent identity: component2052786
display id: component2052786
version: 1
top level: BBa_K256029
access: public
definition: GFP

BBa_B0012
type: Component
persistent identity: component2052789
display id: component2052789
version: 1
top level: BBa_K256029
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0034
type: Component
persistent identity: component2052784
display id: component2052784
version: 1
top level: BBa_K256029
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation2052776
display id: annotation2052776
version: 1
top level: BBa_K256029
location: https://synbiohub.org/public/igem/BBa_K256029/annotation2052776/range2052776/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_K256029/annotation2052776/range2052776/1
type: Range
persistent identity: range2052776
display id: range2052776
version: 1
direction: direction/forward
top level: BBa_K256029
start: 1
end: 200
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2052784
display id: annotation2052784
version: 1
top level: BBa_K256029
location: https://synbiohub.org/public/igem/BBa_K256029/annotation2052784/range2052784/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K256029/annotation2052784/range2052784/1
type: Range
persistent identity: range2052784
display id: range2052784
version: 1
direction: direction/reverse
top level: BBa_K256029
start: 209
end: 220
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2052787
display id: annotation2052787
version: 1
top level: BBa_K256029
location: https://synbiohub.org/public/igem/BBa_K256029/annotation2052787/range2052787/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K256029/annotation2052787/range2052787/1
type: Range
persistent identity: range2052787
display id: range2052787
version: 1
direction: direction/forward
top level: BBa_K256029
start: 955
end: 1034
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation2052786
display id: annotation2052786
version: 1
top level: BBa_K256029
location: https://synbiohub.org/public/igem/BBa_K256029/annotation2052786/range2052786/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_K256029/annotation2052786/range2052786/1
type: Range
persistent identity: range2052786
display id: range2052786
version: 1
direction: direction/reverse
top level: BBa_K256029
start: 227
end: 946
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2052789
display id: annotation2052789
version: 1
top level: BBa_K256029
location: https://synbiohub.org/public/igem/BBa_K256029/annotation2052789/range2052789/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K256029/annotation2052789/range2052789/1
type: Range
persistent identity: range2052789
display id: range2052789
version: 1
direction: direction/forward
top level: BBa_K256029
start: 1043
end: 1083
orientation: inline

RD29A + Ko
type: ComponentDefinition
persistent identity: BBa K414000
display id: BBa_K414000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RD29A Promoter + Strong Plant Kozak (RBS) + RFP
creator: Elaine Bersaba
created: 2010-08-31T11:00:00Z
modified: 2015-06-11T01:50:18Z
discontinued: false
dominant: false
experience: experience/None
group u list: _534_
m user id: 4206
owner id: 6837
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: a
mutable notes: a
mutable provenance: a
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: RD29A
sequence annotation: Kpn I
sequence annotation: Translational Stop 2x
sequence annotation: RFP start codon
sequence annotation: Kozak
sequence annotation: RFP
sequence: https://synbiohub.org/public/igem/BBa_K414000_sequence/1

RD29A
type: SequenceAnnotation
persistent identity: annotation2086059
display id: annotation2086059
version: 1
top level: RD29A + Ko
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K414000/annotation2086059/range2086059/1

https://synbiohub.org/public/igem/BBa_K414000/annotation2086059/range2086059/1
type: Range
persistent identity: range2086059
display id: range2086059
version: 1
direction: direction/forward
top level: RD29A + Ko
start: 1
end: 836
orientation: inline

Kpn I
type: SequenceAnnotation
persistent identity: annotation2086058
display id: annotation2086058
version: 1
top level: RD29A + Ko
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K414000/annotation2086058/range2086058/1

https://synbiohub.org/public/igem/BBa_K414000/annotation2086058/range2086058/1
type: Range
persistent identity: range2086058
display id: range2086058
version: 1
direction: direction/forward
top level: RD29A + Ko
start: 837
end: 842
orientation: inline

Translational Stop 2x
type: SequenceAnnotation
persistent identity: annotation2087834
display id: annotation2087834
version: 1
top level: RD29A + Ko
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K414000/annotation2087834/range2087834/1

https://synbiohub.org/public/igem/BBa_K414000/annotation2087834/range2087834/1
type: Range
persistent identity: range2087834
display id: range2087834
version: 1
direction: direction/forward
top level: RD29A + Ko
start: 1530
end: 1535
orientation: inline

RFP start codon
type: SequenceAnnotation
persistent identity: annotation2086056
display id: annotation2086056
version: 1
top level: RD29A + Ko
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K414000/annotation2086056/range2086056/1

https://synbiohub.org/public/igem/BBa_K414000/annotation2086056/range2086056/1
type: Range
persistent identity: range2086056
display id: range2086056
version: 1
direction: direction/forward
top level: RD29A + Ko
start: 855
end: 857
orientation: inline

Kozak
type: SequenceAnnotation
persistent identity: annotation2086057
display id: annotation2086057
version: 1
top level: RD29A + Ko
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K414000/annotation2086057/range2086057/1

https://synbiohub.org/public/igem/BBa_K414000/annotation2086057/range2086057/1
type: Range
persistent identity: range2086057
display id: range2086057
version: 1
direction: direction/forward
top level: RD29A + Ko
start: 843
end: 854
orientation: inline

RFP
type: SequenceAnnotation
persistent identity: annotation2086060
display id: annotation2086060
version: 1
top level: RD29A + Ko
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K414000/annotation2086060/range2086060/1

https://synbiohub.org/public/igem/BBa_K414000/annotation2086060/range2086060/1
type: Range
persistent identity: range2086060
display id: range2086060
version: 1
direction: direction/forward
top level: RD29A + Ko
start: 855
end: 1529
orientation: inline

BBa_K332032
type: ComponentDefinition
persistent identity: BBa K332032
display id: BBa_K332032
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Constitutive promoter+37℃ induced RBS+tetR+double terminator+Ptet+RBS+GFP+double terminator
creator: KUO, CHIA-CHUN
created: 2010-10-21T11:00:00Z
modified: 2015-05-08T01:12:05Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _452_
m user id: 0
owner id: 7059
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: We want to utilize a temperature-regulated mechanism to control the expression period of a crystal protein. Therefore, we took advantage of the interaction between 37?? C induced RBS, tetR gene and pTet promoter to create the temperature regulating circuit. The following is a description of our prescribed strategy. First, we use the constitutive promoter, 37?? C induced RBS (K115002), the tetR gene and terminators. We will call this part the ???Regulator???.
mutable notes: none
mutable provenance: none
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_J61048
component: BBa_E0040
component: BBa_B0034
component: BBa_K332031
component: BBa_R0040
sequence annotation: BBa_K332031
sequence annotation: BBa_E0040
sequence annotation: BBa_B0034
sequence annotation: BBa_J61048
sequence annotation: BBa_R0040
sequence: https://synbiohub.org/public/igem/BBa_K332032_sequence/1

BBa_J61048
type: Component
persistent identity: component2273256
display id: component2273256
version: 1
top level: BBa_K332032
access: public
definition: https://synbiohub.org/public/igem/BBa_J61048/1

BBa_E0040
type: Component
persistent identity: component2273255
display id: component2273255
version: 1
top level: BBa_K332032
access: public
definition: GFP

BBa_B0034
type: Component
persistent identity: component2273253
display id: component2273253
version: 1
top level: BBa_K332032
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K332031
type: Component
persistent identity: component2273246
display id: component2273246
version: 1
top level: BBa_K332032
access: public
definition: https://synbiohub.org/public/igem/BBa_K332031/1

BBa_R0040
type: Component
persistent identity: component2273247
display id: component2273247
version: 1
top level: BBa_K332032
access: public
definition: https://synbiohub.org/public/igem/BBa_R0040/1

BBa_K332031
type: SequenceAnnotation
persistent identity: annotation2273246
display id: annotation2273246
version: 1
top level: BBa_K332032
location: https://synbiohub.org/public/igem/BBa_K332032/annotation2273246/range2273246/1
component: BBa_K332031

https://synbiohub.org/public/igem/BBa_K332032/annotation2273246/range2273246/1
type: Range
persistent identity: range2273246
display id: range2273246
version: 1
direction: direction/reverse
top level: BBa_K332032
start: 1
end: 907
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation2273255
display id: annotation2273255
version: 1
top level: BBa_K332032
location: https://synbiohub.org/public/igem/BBa_K332032/annotation2273255/range2273255/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_K332032/annotation2273255/range2273255/1
type: Range
persistent identity: range2273255
display id: range2273255
version: 1
direction: direction/reverse
top level: BBa_K332032
start: 996
end: 1715
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2273253
display id: annotation2273253
version: 1
top level: BBa_K332032
location: https://synbiohub.org/public/igem/BBa_K332032/annotation2273253/range2273253/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K332032/annotation2273253/range2273253/1
type: Range
persistent identity: range2273253
display id: range2273253
version: 1
direction: direction/reverse
top level: BBa_K332032
start: 978
end: 989
orientation: inline

BBa_J61048
type: SequenceAnnotation
persistent identity: annotation2273256
display id: annotation2273256
version: 1
top level: BBa_K332032
location: https://synbiohub.org/public/igem/BBa_K332032/annotation2273256/range2273256/1
component: BBa_J61048

https://synbiohub.org/public/igem/BBa_K332032/annotation2273256/range2273256/1
type: Range
persistent identity: range2273256
display id: range2273256
version: 1
direction: direction/reverse
top level: BBa_K332032
start: 1724
end: 1836
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2273247
display id: annotation2273247
version: 1
top level: BBa_K332032
location: https://synbiohub.org/public/igem/BBa_K332032/annotation2273247/range2273247/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_K332032/annotation2273247/range2273247/1
type: Range
persistent identity: range2273247
display id: range2273247
version: 1
direction: direction/forward
top level: BBa_K332032
start: 916
end: 969
orientation: inline

BBa_K1486011
type: ComponentDefinition
persistent identity: BBa K1486011
display id: BBa_K1486011
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CxpR & Split IFP1.4 [Cterm + Nterm]
creator: iGem EPFL 2014
created: 2014-08-26T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part has two fused proteins downstream of an arabinose-inducible promoter: CpxR-IFP[1] and CpxR-IFP[2]. This construct was made in all orientations: - CpxR-IFP[1] + CpxR-IFP[2] (BBa_K1286008) - IFP[1]-CpxR + CpxR-IFP[2] (BBa_K1486010) - CpxR-IFP[1] + IFP[2]-CpxR (BBa_K1486011)
mutable notes: All orientations were made, so all dimerizations could be tested (see main page for biobrick numbers). Flexible linkers were used for each of the fused proteins, and an RBS was put before each one of them.
mutable provenance: From Michnick Article. To Edit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_I0500
component: BBa_K1486004
component: BBa_K1486000
component: BBa_B0034
component: BBa_K1486006
component: BBa_K1486007
sequence annotation: BBa_B0034
sequence annotation: BBa_K1486006
sequence annotation: BBa_K1486000
sequence annotation: BBa_K1486004
sequence annotation: BBa_I0500
sequence annotation: BBa_K1486007
sequence: https://synbiohub.org/public/igem/BBa_K1486011_sequence/1

BBa_I0500
type: Component
persistent identity: component2382235
display id: component2382235
version: 1
top level: BBa_K1486011
access: public
definition: https://synbiohub.org/public/igem/BBa_I0500/1

BBa_K1486004
type: Component
persistent identity: component2382242
display id: component2382242
version: 1
top level: BBa_K1486011
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486004/1

BBa_K1486000
type: Component
persistent identity: component2382254
display id: component2382254
version: 1
top level: BBa_K1486011
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486000/1

BBa_B0034
type: Component
persistent identity: component2382237
display id: component2382237
version: 1
top level: BBa_K1486011
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K1486000
type: Component
persistent identity: component2382241
display id: component2382241
version: 1
top level: BBa_K1486011
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486000/1

BBa_K1486004
type: Component
persistent identity: component2382250
display id: component2382250
version: 1
top level: BBa_K1486011
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486004/1

BBa_K1486006
type: Component
persistent identity: component2382243
display id: component2382243
version: 1
top level: BBa_K1486011
access: public
definition: BBa_K1486006

BBa_B0034
type: Component
persistent identity: component2382245
display id: component2382245
version: 1
top level: BBa_K1486011
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K1486007
type: Component
persistent identity: component2382249
display id: component2382249
version: 1
top level: BBa_K1486011
access: public
definition: BBa_K1486007

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2382245
display id: annotation2382245
version: 1
top level: BBa_K1486011
location: https://synbiohub.org/public/igem/BBa_K1486011/annotation2382245/range2382245/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1486011/annotation2382245/range2382245/1
type: Range
persistent identity: range2382245
display id: range2382245
version: 1
direction: direction/reverse
top level: BBa_K1486011
start: 2537
end: 2548
orientation: inline

BBa_K1486006
type: SequenceAnnotation
persistent identity: annotation2382243
display id: annotation2382243
version: 1
top level: BBa_K1486011
location: https://synbiohub.org/public/igem/BBa_K1486011/annotation2382243/range2382243/1
component: BBa_K1486006

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type: Range
persistent identity: range2382243
display id: range2382243
version: 1
direction: direction/reverse
top level: BBa_K1486011
start: 1955
end: 2536
orientation: inline

BBa_K1486000
type: SequenceAnnotation
persistent identity: annotation2382254
display id: annotation2382254
version: 1
top level: BBa_K1486011
location: https://synbiohub.org/public/igem/BBa_K1486011/annotation2382254/range2382254/1
component: BBa_K1486000

https://synbiohub.org/public/igem/BBa_K1486011/annotation2382254/range2382254/1
type: Range
persistent identity: range2382254
display id: range2382254
version: 1
direction: direction/reverse
top level: BBa_K1486011
start: 3158
end: 3859
orientation: inline

BBa_K1486004
type: SequenceAnnotation
persistent identity: annotation2382250
display id: annotation2382250
version: 1
top level: BBa_K1486011
location: https://synbiohub.org/public/igem/BBa_K1486011/annotation2382250/range2382250/1
component: BBa_K1486004

https://synbiohub.org/public/igem/BBa_K1486011/annotation2382250/range2382250/1
type: Range
persistent identity: range2382250
display id: range2382250
version: 1
direction: direction/reverse
top level: BBa_K1486011
start: 3128
end: 3157
orientation: inline

BBa_I0500
type: SequenceAnnotation
persistent identity: annotation2382235
display id: annotation2382235
version: 1
top level: BBa_K1486011
location: https://synbiohub.org/public/igem/BBa_K1486011/annotation2382235/range2382235/1
component: BBa_I0500

https://synbiohub.org/public/igem/BBa_K1486011/annotation2382235/range2382235/1
type: Range
persistent identity: range2382235
display id: range2382235
version: 1
direction: direction/reverse
top level: BBa_K1486011
start: 1
end: 1210
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2382237
display id: annotation2382237
version: 1
top level: BBa_K1486011
location: https://synbiohub.org/public/igem/BBa_K1486011/annotation2382237/range2382237/1
component: BBa_B0034

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type: Range
persistent identity: range2382237
display id: range2382237
version: 1
direction: direction/reverse
top level: BBa_K1486011
start: 1211
end: 1222
orientation: inline

BBa_K1486000
type: SequenceAnnotation
persistent identity: annotation2382241
display id: annotation2382241
version: 1
top level: BBa_K1486011
location: https://synbiohub.org/public/igem/BBa_K1486011/annotation2382241/range2382241/1
component: BBa_K1486000

https://synbiohub.org/public/igem/BBa_K1486011/annotation2382241/range2382241/1
type: Range
persistent identity: range2382241
display id: range2382241
version: 1
direction: direction/reverse
top level: BBa_K1486011
start: 1223
end: 1924
orientation: inline

BBa_K1486007
type: SequenceAnnotation
persistent identity: annotation2382249
display id: annotation2382249
version: 1
top level: BBa_K1486011
location: https://synbiohub.org/public/igem/BBa_K1486011/annotation2382249/range2382249/1
component: BBa_K1486007

https://synbiohub.org/public/igem/BBa_K1486011/annotation2382249/range2382249/1
type: Range
persistent identity: range2382249
display id: range2382249
version: 1
direction: direction/reverse
top level: BBa_K1486011
start: 2549
end: 3127
orientation: inline

BBa_K1486004
type: SequenceAnnotation
persistent identity: annotation2382242
display id: annotation2382242
version: 1
top level: BBa_K1486011
location: https://synbiohub.org/public/igem/BBa_K1486011/annotation2382242/range2382242/1
component: BBa_K1486004

https://synbiohub.org/public/igem/BBa_K1486011/annotation2382242/range2382242/1
type: Range
persistent identity: range2382242
display id: range2382242
version: 1
direction: direction/reverse
top level: BBa_K1486011
start: 1925
end: 1954
orientation: inline

EYFP
type: ComponentDefinition
persistent identity: BBa K1319000
display id: BBa_K1319000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RFC[25] version of E0030
creator: Michael Osthege, Florian Gohr
created: 2014-08-10T11:00:00Z
modified: 2015-05-08T01:09:50Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1694_
m user id: 0
owner id: 19522
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part is a RFC[25]-compatible version of BBa_E0030. The start and stop codons have been removed to make it RFC[25]-compatible and the part is flanked by the RFC[25] prefix- and suffix-sequences.
mutable notes: start and stop-codons have been removed as they are present in the RFC[25] prefix/suffix sequences.
mutable provenance: The part is an improved version of BBa_E0030
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: EYFP
sequence: https://synbiohub.org/public/igem/BBa_K1319000_sequence/1

EYFP
type: SequenceAnnotation
persistent identity: annotation2380384
display id: annotation2380384
version: 1
top level: EYFP
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1319000/annotation2380384/range2380384/1

https://synbiohub.org/public/igem/BBa_K1319000/annotation2380384/range2380384/1
type: Range
persistent identity: range2380384
display id: range2380384
version: 1
direction: direction/forward
top level: EYFP
start: 1
end: 711
orientation: inline

YFP
type: ComponentDefinition
persistent identity: BBa K592101
display id: BBa_K592101
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Yellow Fluorescent Protein (YFP)
creator: Erik Lundin
created: 2011-09-20T11:00:00Z
modified: 2016-01-25T04:47:08Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _763_
m user id: 4206
owner id: 9827
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: Yellow Fluorescent Protein derived from A. victoria GFP. This fluorescent protein is cloned from the pZE12-YFP plasmid used by Elowitz (see reference). The original sequence was made by the The Yeast Resource Center (YRC) based at the University of Washington in Seattle, Washington.
mutable notes: This fluorescent protein was cloned from the pZE12-YFP plasmid used by Elowitz (see reference).
mutable provenance: Elowitz, M. B., A. J. Levine, et al. (2002). "Stochastic gene expression in a single cell." Science 297(5584): 1183-6.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: YFP
sequence: https://synbiohub.org/public/igem/BBa_K592101_sequence/1

YFP
type: SequenceAnnotation
persistent identity: annotation2137506
display id: annotation2137506
version: 1
top level: YFP
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K592101/annotation2137506/range2137506/1

https://synbiohub.org/public/igem/BBa_K592101/annotation2137506/range2137506/1
type: Range
persistent identity: range2137506
display id: range2137506
version: 1
direction: direction/forward
top level: YFP
start: 1
end: 714
orientation: inline

PlsiR.GFP
type: ComponentDefinition
persistent identity: BBa K1725401
display id: BBa_K1725401
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PlsiR + B0034 + GFP
creator: Andrey Filipov
created: 2015-09-07T11:00:00Z
modified: 2015-09-08T11:23:40Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2146_
m user id: 21308
owner id: 21308
owning group id: 9
status: status/Planning
bookmark: false
mutable description: The promoter activated by the release of UirR (K1725420) from the transmembrane protein UirS (K1725410) in response to unidirectional UV-A light is upstream of GFP with the B0034 RBS (BBa_I13500). This allows activity in response to UV-A to be measured. For the promoter to be activated UirR and UirS must be present and the cell has to be exposed to UV-A.
mutable notes: The promoter, including the BioBrick prefix and suffix, was ordered as a G-block. It was ligated to I13500 after restriction that was followed by an Azure-A-based gel extraction.
mutable provenance: The promoter comes from the genome of the unicellular cyanobacterium Synechocystis sp. PCC6803.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_K1725400
component: BBa_I13500
sequence annotation: BBa_K1725400
sequence annotation: BBa_I13500
sequence: https://synbiohub.org/public/igem/BBa_K1725401_sequence/1

BBa_K1725400
type: Component
persistent identity: component2446999
display id: component2446999
version: 1
top level: PlsiR.GFP
access: public
definition: https://synbiohub.org/public/igem/BBa_K1725400/1

BBa_I13500
type: Component
persistent identity: component2447003
display id: component2447003
version: 1
top level: PlsiR.GFP
access: public
definition: https://synbiohub.org/public/igem/BBa_I13500/1

BBa_K1725400
type: SequenceAnnotation
persistent identity: annotation2446999
display id: annotation2446999
version: 1
top level: PlsiR.GFP
location: https://synbiohub.org/public/igem/BBa_K1725401/annotation2446999/range2446999/1
component: BBa_K1725400

https://synbiohub.org/public/igem/BBa_K1725401/annotation2446999/range2446999/1
type: Range
persistent identity: range2446999
display id: range2446999
version: 1
direction: direction/reverse
top level: PlsiR.GFP
start: 1
end: 534
orientation: inline

BBa_I13500
type: SequenceAnnotation
persistent identity: annotation2447003
display id: annotation2447003
version: 1
top level: PlsiR.GFP
location: https://synbiohub.org/public/igem/BBa_K1725401/annotation2447003/range2447003/1
component: BBa_I13500

https://synbiohub.org/public/igem/BBa_K1725401/annotation2447003/range2447003/1
type: Range
persistent identity: range2447003
display id: range2447003
version: 1
direction: direction/reverse
top level: PlsiR.GFP
start: 543
end: 1280
orientation: inline

BBa_K106005
type: ComponentDefinition
persistent identity: BBa K106005
display id: BBa_K106005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: mCherry, Aar1 BD part
creator: Ryan Quan
created: 2008-10-21T11:00:00Z
modified: 2016-01-26T09:49:02Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _226_
m user id: 4206
owner id: 3421
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This is the coding sequence for mCherry with AB ends for Aar1 cloning.
mutable notes: Since this is an BD part, there is no ATG but there is a stop codon. For more information on AarI cloning, please refer to the UCSF 2008 igem page.
mutable provenance: none
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K106005_sequence/1

BBa_K1197008
type: ComponentDefinition
persistent identity: BBa K1197008
display id: BBa_K1197008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Pveg (K823003) + RBS (SpoVG) + RFP + DT
creator: Emre İlpars
created: 2013-09-21T11:00:00Z
modified: 2015-06-08T11:17:06Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1511_
m user id: 4206
owner id: 12422
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: .
mutable notes: .
mutable provenance: .
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K823003
component: BBa_K606013
sequence annotation: BBa_K823003
sequence annotation: BBa_K606013
sequence: https://synbiohub.org/public/igem/BBa_K1197008_sequence/1

BBa_K823003
type: Component
persistent identity: component2359764
display id: component2359764
version: 1
top level: BBa_K1197008
access: public
definition: https://synbiohub.org/public/igem/BBa_K823003/1

BBa_K606013
type: Component
persistent identity: component2359778
display id: component2359778
version: 1
top level: BBa_K1197008
access: public
definition: https://synbiohub.org/public/igem/BBa_K606013/1

BBa_K823003
type: SequenceAnnotation
persistent identity: annotation2359764
display id: annotation2359764
version: 1
top level: BBa_K1197008
location: https://synbiohub.org/public/igem/BBa_K1197008/annotation2359764/range2359764/1
component: BBa_K823003

https://synbiohub.org/public/igem/BBa_K1197008/annotation2359764/range2359764/1
type: Range
persistent identity: range2359764
display id: range2359764
version: 1
direction: direction/reverse
top level: BBa_K1197008
start: 1
end: 237
orientation: inline

BBa_K606013
type: SequenceAnnotation
persistent identity: annotation2359778
display id: annotation2359778
version: 1
top level: BBa_K1197008
location: https://synbiohub.org/public/igem/BBa_K1197008/annotation2359778/range2359778/1
component: BBa_K606013

https://synbiohub.org/public/igem/BBa_K1197008/annotation2359778/range2359778/1
type: Range
persistent identity: range2359778
display id: range2359778
version: 1
direction: direction/reverse
top level: BBa_K1197008
start: 246
end: 1106
orientation: inline

BBa_K283005
type: ComponentDefinition
persistent identity: BBa K283005
display id: BBa_K283005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: lpp_ompA_eGFP_streptavidin
creator: Yu bin, Jiang QinQin
created: 2009-10-12T11:00:00Z
modified: 2015-05-08T01:11:47Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _383_
m user id: 0
owner id: 5494
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: This part consists of a fusion protein Lpp-rbs-protein under the pLac promoter. The Lpp is a signal peptide. The ompA domain of the fusion directs the protein to the surface membrane. The pLac promoter can be repressed by LacI protein. This part lacks a terminator.
mutable notes: BBa_K283005
mutable provenance: The pLac promoter, rbs sequence are obtained from the registry The fusion protein coding sequence is self-designed
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_J36837
component: BBa_K283009
component: BBa_J36835
sequence annotation: BBa_K283009
sequence annotation: BBa_J36837
sequence annotation: BBa_J36835
sequence: https://synbiohub.org/public/igem/BBa_K283005_sequence/1

BBa_J36837
type: Component
persistent identity: component2055625
display id: component2055625
version: 1
top level: BBa_K283005
access: public
definition: https://synbiohub.org/public/igem/BBa_J36837/1

BBa_K283009
type: Component
persistent identity: component2055627
display id: component2055627
version: 1
top level: BBa_K283005
access: public
definition: https://synbiohub.org/public/igem/BBa_K283009/1

BBa_J36835
type: Component
persistent identity: component2055624
display id: component2055624
version: 1
top level: BBa_K283005
access: public
definition: https://synbiohub.org/public/igem/BBa_J36835/1

BBa_K283009
type: SequenceAnnotation
persistent identity: annotation2055627
display id: annotation2055627
version: 1
top level: BBa_K283005
location: https://synbiohub.org/public/igem/BBa_K283005/annotation2055627/range2055627/1
component: BBa_K283009

https://synbiohub.org/public/igem/BBa_K283005/annotation2055627/range2055627/1
type: Range
persistent identity: range2055627
display id: range2055627
version: 1
direction: direction/reverse
top level: BBa_K283005
start: 430
end: 1533
orientation: inline

BBa_J36837
type: SequenceAnnotation
persistent identity: annotation2055625
display id: annotation2055625
version: 1
top level: BBa_K283005
location: https://synbiohub.org/public/igem/BBa_K283005/annotation2055625/range2055625/1
component: BBa_J36837

https://synbiohub.org/public/igem/BBa_K283005/annotation2055625/range2055625/1
type: Range
persistent identity: range2055625
display id: range2055625
version: 1
direction: direction/reverse
top level: BBa_K283005
start: 88
end: 429
orientation: inline

BBa_J36835
type: SequenceAnnotation
persistent identity: annotation2055624
display id: annotation2055624
version: 1
top level: BBa_K283005
location: https://synbiohub.org/public/igem/BBa_K283005/annotation2055624/range2055624/1
component: BBa_J36835

https://synbiohub.org/public/igem/BBa_K283005/annotation2055624/range2055624/1
type: Range
persistent identity: range2055624
display id: range2055624
version: 1
direction: direction/reverse
top level: BBa_K283005
start: 1
end: 87
orientation: inline

BBa_K1679003
type: ComponentDefinition
persistent identity: BBa K1679003
display id: BBa_K1679003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: GFP Reporter
creator: Qikai Qin
created: 2015-09-14T11:00:00Z
modified: 2015-09-18T08:56:00Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2097_
m user id: 21726
owner id: 21726
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This is a device containing a promoter (BBa_J23117),a RBS (BBa_B0034), a GFP coding sequence (BBa_E0040) and two terminators (BBa_B0010, BBa_B0012) on the pSB1C3, which can be easily transformed into E.coli and produce GFP (mut3b). This is a GFP expression device, which can express GFP (mut3b) without any induction.
mutable notes: This is one of 2015 InterLab Study required devices. The strength of the promoter is weak.
mutable provenance: BBa_J23117 BBa_I13504
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_J23117
component: BBa_I13504
sequence annotation: BBa_I13504
sequence annotation: BBa_J23117
sequence: https://synbiohub.org/public/igem/BBa_K1679003_sequence/1

BBa_J23117
type: Component
persistent identity: component2456779
display id: component2456779
version: 1
top level: BBa_K1679003
access: public
definition: https://synbiohub.org/public/igem/BBa_J23117/1

BBa_I13504
type: Component
persistent identity: component2456789
display id: component2456789
version: 1
top level: BBa_K1679003
access: public
definition: https://synbiohub.org/public/igem/BBa_I13504/1

BBa_I13504
type: SequenceAnnotation
persistent identity: annotation2456789
display id: annotation2456789
version: 1
top level: BBa_K1679003
location: https://synbiohub.org/public/igem/BBa_K1679003/annotation2456789/range2456789/1
component: BBa_I13504

https://synbiohub.org/public/igem/BBa_K1679003/annotation2456789/range2456789/1
type: Range
persistent identity: range2456789
display id: range2456789
version: 1
direction: direction/reverse
top level: BBa_K1679003
start: 44
end: 918
orientation: inline

BBa_J23117
type: SequenceAnnotation
persistent identity: annotation2456779
display id: annotation2456779
version: 1
top level: BBa_K1679003
location: https://synbiohub.org/public/igem/BBa_K1679003/annotation2456779/range2456779/1
component: BBa_J23117

https://synbiohub.org/public/igem/BBa_K1679003/annotation2456779/range2456779/1
type: Range
persistent identity: range2456779
display id: range2456779
version: 1
direction: direction/reverse
top level: BBa_K1679003
start: 1
end: 35
orientation: inline

BBa_K1486035
type: ComponentDefinition
persistent identity: BBa K1486035
display id: BBa_K1486035
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Yeast Optimized sfGFP-C + ADH1 terminator + CaUra3 Cassette
creator: EPFL iGem team 2014
created: 2014-09-26T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: To edit
mutable notes: To edit
mutable provenance: To edit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000324
role: partType/Tag
component: BBa_K1486034
component: BBa_K1486037
component: BBa_K1486031
component: BBa_K1486025
sequence annotation: BBa_K1486031
sequence annotation: BBa_K1486025
sequence annotation: BBa_K1486034
sequence annotation: BBa_K1486037
sequence: https://synbiohub.org/public/igem/BBa_K1486035_sequence/1

BBa_K1486034
type: Component
persistent identity: component2418330
display id: component2418330
version: 1
top level: BBa_K1486035
access: public
definition: BBa_K1486034

BBa_K1486037
type: Component
persistent identity: component2418328
display id: component2418328
version: 1
top level: BBa_K1486035
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486037/1

BBa_K1486031
type: Component
persistent identity: component2418335
display id: component2418335
version: 1
top level: BBa_K1486035
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486031/1

BBa_K1486025
type: Component
persistent identity: component2418331
display id: component2418331
version: 1
top level: BBa_K1486035
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486025/1

BBa_K1486031
type: SequenceAnnotation
persistent identity: annotation2418335
display id: annotation2418335
version: 1
top level: BBa_K1486035
location: https://synbiohub.org/public/igem/BBa_K1486035/annotation2418335/range2418335/1
component: BBa_K1486031

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type: Range
persistent identity: range2418335
display id: range2418335
version: 1
direction: direction/reverse
top level: BBa_K1486035
start: 303
end: 1662
orientation: inline

BBa_K1486025
type: SequenceAnnotation
persistent identity: annotation2418331
display id: annotation2418331
version: 1
top level: BBa_K1486035
location: https://synbiohub.org/public/igem/BBa_K1486035/annotation2418331/range2418331/1
component: BBa_K1486025

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type: Range
persistent identity: range2418331
display id: range2418331
version: 1
direction: direction/reverse
top level: BBa_K1486035
start: 115
end: 302
orientation: inline

BBa_K1486034
type: SequenceAnnotation
persistent identity: annotation2418330
display id: annotation2418330
version: 1
top level: BBa_K1486035
location: https://synbiohub.org/public/igem/BBa_K1486035/annotation2418330/range2418330/1
component: BBa_K1486034

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type: Range
persistent identity: range2418330
display id: range2418330
version: 1
direction: direction/reverse
top level: BBa_K1486035
start: 40
end: 114
orientation: inline

BBa_K1486037
type: SequenceAnnotation
persistent identity: annotation2418328
display id: annotation2418328
version: 1
top level: BBa_K1486035
location: https://synbiohub.org/public/igem/BBa_K1486035/annotation2418328/range2418328/1
component: BBa_K1486037

https://synbiohub.org/public/igem/BBa_K1486035/annotation2418328/range2418328/1
type: Range
persistent identity: range2418328
display id: range2418328
version: 1
direction: direction/reverse
top level: BBa_K1486035
start: 1
end: 39
orientation: inline

BBa_K106028
type: ComponentDefinition
persistent identity: BBa K106028
display id: BBa_K106028
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: GFP, AarI AB part
creator: Andrej Ondracka
created: 2008-10-24T11:00:00Z
modified: 2016-01-26T09:49:32Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _226_
m user id: 4206
owner id: 3416
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This is coding sequence of GFP (yeast optimized codons).
mutable notes: .
mutable provenance: .
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K106028_sequence/1

mRFP1
type: ComponentDefinition
persistent identity: BBa E2050
display id: BBa_E2050
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: derivative of mRFP1, yeast-optimized
creator: ryu
created: 2005-01-24T12:00:00Z
modified: 2016-01-25T02:35:54Z
discontinued: false
dominant: false
experience: experience/None
group u list: _8_
m user id: 4206
owner id: 230
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: mRFP derivative. Ex548nm/Em562. yeast codon optimized.
mutable notes: Contains N- and C-terminal linker sequences (with homology to biobrick parts BBa_E2060 (mCherry), BBa_E2020 (Cerulean CFP), and BBa_E2030 (Venus YFP) to facilitate color swapping in yeast. Adds N-"MATSG" and "GSGTA"-C. to published amino acid sequence. Double TAATAA stop codon. Missing EcoRI, HindIII, NotI, NdeI, XhoI, RsrII, BamHI, NcoI, BglI, SpeI, XbaI, and PstI. except for 5' and 3' ends no significant sequence identity runs to mCherry (BBa_E2060).
mutable provenance: Shaner et al, 2004. Nat Biotech (22):1567-1571
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Help:Barcodes
sequence annotation: mOrange
sequence annotation: MATSG linker
sequence annotation: GSGTA linker
sequence: https://synbiohub.org/public/igem/BBa_E2050_sequence/1

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2214024
display id: annotation2214024
version: 1
top level: mRFP1
role: SO:0000807
role: feature/barcode
location: https://synbiohub.org/public/igem/BBa_E2050/annotation2214024/range2214024/1

https://synbiohub.org/public/igem/BBa_E2050/annotation2214024/range2214024/1
type: Range
persistent identity: range2214024
display id: range2214024
version: 1
direction: direction/forward
top level: mRFP1
start: 745
end: 769
orientation: inline

mOrange
type: SequenceAnnotation
persistent identity: annotation1431920
display id: annotation1431920
version: 1
top level: mRFP1
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_E2050/annotation1431920/range1431920/1

https://synbiohub.org/public/igem/BBa_E2050/annotation1431920/range1431920/1
type: Range
persistent identity: range1431920
display id: range1431920
version: 1
direction: direction/forward
top level: mRFP1
start: 1
end: 738
orientation: inline

MATSG linker
type: SequenceAnnotation
persistent identity: annotation1431915
display id: annotation1431915
version: 1
top level: mRFP1
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_E2050/annotation1431915/range1431915/1

https://synbiohub.org/public/igem/BBa_E2050/annotation1431915/range1431915/1
type: Range
persistent identity: range1431915
display id: range1431915
version: 1
direction: direction/forward
top level: mRFP1
start: 1
end: 15
orientation: inline

GSGTA linker
type: SequenceAnnotation
persistent identity: annotation1431919
display id: annotation1431919
version: 1
top level: mRFP1
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_E2050/annotation1431919/range1431919/1

https://synbiohub.org/public/igem/BBa_E2050/annotation1431919/range1431919/1
type: Range
persistent identity: range1431919
display id: range1431919
version: 1
direction: direction/forward
top level: mRFP1
start: 724
end: 738
orientation: inline

BBa_K1197009
type: ComponentDefinition
persistent identity: BBa K1197009
display id: BBa_K1197009
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Phyp + RBS (SpoVG) + RFP + DT
creator: Emre İlpars
created: 2013-09-21T11:00:00Z
modified: 2015-06-08T11:40:52Z
discontinued: false
dominant: false
group u list: _1511_
m user id: 0
m user id: 4206
owner id: 12422
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: .
mutable notes: .
mutable provenance: .
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K606040
component: BBa_K606013
sequence annotation: BBa_K606013
sequence annotation: BBa_K606040
sequence: https://synbiohub.org/public/igem/BBa_K1197009_sequence/1

BBa_K606040
type: Component
persistent identity: component2359802
display id: component2359802
version: 1
top level: BBa_K1197009
access: public
definition: https://synbiohub.org/public/igem/BBa_K606040/1

BBa_K606013
type: Component
persistent identity: component2359816
display id: component2359816
version: 1
top level: BBa_K1197009
access: public
definition: https://synbiohub.org/public/igem/BBa_K606013/1

BBa_K606013
type: SequenceAnnotation
persistent identity: annotation2359816
display id: annotation2359816
version: 1
top level: BBa_K1197009
location: https://synbiohub.org/public/igem/BBa_K1197009/annotation2359816/range2359816/1
component: BBa_K606013

https://synbiohub.org/public/igem/BBa_K1197009/annotation2359816/range2359816/1
type: Range
persistent identity: range2359816
display id: range2359816
version: 1
direction: direction/reverse
top level: BBa_K1197009
start: 101
end: 961
orientation: inline

BBa_K606040
type: SequenceAnnotation
persistent identity: annotation2359802
display id: annotation2359802
version: 1
top level: BBa_K1197009
location: https://synbiohub.org/public/igem/BBa_K1197009/annotation2359802/range2359802/1
component: BBa_K606040

https://synbiohub.org/public/igem/BBa_K1197009/annotation2359802/range2359802/1
type: Range
persistent identity: range2359802
display id: range2359802
version: 1
direction: direction/reverse
top level: BBa_K1197009
start: 1
end: 92
orientation: inline

BBa_K1680020
type: ComponentDefinition
persistent identity: BBa K1680020
display id: BBa_K1680020
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Cre-dronpa fusion
creator: Nicolai von Kuegelgen
created: 2015-09-16T11:00:00Z
modified: 2016-01-26T10:16:19Z
discontinued: false
dominant: false
group u list: _2098_
m user id: 4206
owner id: 26366
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Protein coding region for a fusion construct of Cre Recombinase and 145N Dronpa with 15 amino acid linker. Oligomerisation of Dronpa should stop Cre from accessing DNA in the nucleus thereby inhibiting it until light deactivation of dronpa.
mutable notes: 3A assembly using RFC25
mutable provenance: gBlock design by Team Tuebingen
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: K1680003
sequence annotation: K1680007
sequence annotation: K1680006
sequence: https://synbiohub.org/public/igem/BBa_K1680020_sequence/1

K1680003
type: SequenceAnnotation
persistent identity: annotation2477366
display id: annotation2477366
version: 1
top level: BBa_K1680020
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1680020/annotation2477366/range2477366/1

https://synbiohub.org/public/igem/BBa_K1680020/annotation2477366/range2477366/1
type: Range
persistent identity: range2477366
display id: range2477366
version: 1
direction: direction/forward
top level: BBa_K1680020
start: 1089
end: 1113
orientation: inline

K1680003
type: SequenceAnnotation
persistent identity: annotation2477365
display id: annotation2477365
version: 1
top level: BBa_K1680020
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1680020/annotation2477365/range2477365/1

https://synbiohub.org/public/igem/BBa_K1680020/annotation2477365/range2477365/1
type: Range
persistent identity: range2477365
display id: range2477365
version: 1
direction: direction/forward
top level: BBa_K1680020
start: 1
end: 39
orientation: inline

K1680007
type: SequenceAnnotation
persistent identity: annotation2477363
display id: annotation2477363
version: 1
top level: BBa_K1680020
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1680020/annotation2477363/range2477363/1

https://synbiohub.org/public/igem/BBa_K1680020/annotation2477363/range2477363/1
type: Range
persistent identity: range2477363
display id: range2477363
version: 1
direction: direction/forward
top level: BBa_K1680020
start: 40
end: 1068
orientation: inline

K1680006
type: SequenceAnnotation
persistent identity: annotation2477364
display id: annotation2477364
version: 1
top level: BBa_K1680020
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1680020/annotation2477364/range2477364/1

https://synbiohub.org/public/igem/BBa_K1680020/annotation2477364/range2477364/1
type: Range
persistent identity: range2477364
display id: range2477364
version: 1
direction: direction/forward
top level: BBa_K1680020
start: 1114
end: 1782
orientation: inline

BBa_I712028
type: ComponentDefinition
persistent identity: BBa I712028
display id: BBa_I712028
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CherryNLS - synthetic construct monomeric red fluorescent protein with nuclear localization sequence
creator: Marko Bitenc
created: 2007-10-20T11:00:00Z
modified: 2015-08-31T04:07:46Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _130_
m user id: 0
owner id: 1835
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This protein is used to show that T7 polymerase is actually going into the nucleolus, where it can transcribe genes under T7 promotor.
mutable notes: In the original cherry protein there was a PstI restriction site in the middle of the sequence. That is why mutagenesis of this restiction site was necessary.
mutable provenance: From one clone that Gabi gave me - will be further described in the future.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_I712028_sequence/1

106GFP
type: ComponentDefinition
persistent identity: BBa K1679002
display id: BBa_K1679002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: GFP Reporter
creator: Qikai Qin
created: 2015-09-04T11:00:00Z
modified: 2015-09-18T08:48:21Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2097_
m user id: 21726
owner id: 21726
owning group id: 9
status: status/Planning
bookmark: true
mutable description: This is a device containing a promoter (BBa_J23106),a RBS (BBa_B0034), a GFP coding sequence (BBa_E0040) and two terminators (BBa_B0010, BBa_B0012) on the pSB1C3, which can be easily transformed into E.coli and produce GFP (mut3b). This is a GFP expression device, which can express GFP (mut3b) without any induction.
mutable notes: To finish the InterLab Study, and want to make it a well characterized device which can be easily used.
mutable provenance: BBa_J23106 BBa_I13504
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_I13504
component: BBa_J23106
sequence annotation: BBa_J23106
sequence annotation: BBa_I13504
sequence: https://synbiohub.org/public/igem/BBa_K1679002_sequence/1

BBa_I13504
type: Component
persistent identity: component2444402
display id: component2444402
version: 1
top level: 106GFP
access: public
definition: https://synbiohub.org/public/igem/BBa_I13504/1

BBa_J23106
type: Component
persistent identity: component2444392
display id: component2444392
version: 1
top level: 106GFP
access: public
definition: https://synbiohub.org/public/igem/BBa_J23106/1

BBa_J23106
type: SequenceAnnotation
persistent identity: annotation2444392
display id: annotation2444392
version: 1
top level: 106GFP
location: https://synbiohub.org/public/igem/BBa_K1679002/annotation2444392/range2444392/1
component: BBa_J23106

https://synbiohub.org/public/igem/BBa_K1679002/annotation2444392/range2444392/1
type: Range
persistent identity: range2444392
display id: range2444392
version: 1
direction: direction/reverse
top level: 106GFP
start: 1
end: 35
orientation: inline

BBa_I13504
type: SequenceAnnotation
persistent identity: annotation2444402
display id: annotation2444402
version: 1
top level: 106GFP
location: https://synbiohub.org/public/igem/BBa_K1679002/annotation2444402/range2444402/1
component: BBa_I13504

https://synbiohub.org/public/igem/BBa_K1679002/annotation2444402/range2444402/1
type: Range
persistent identity: range2444402
display id: range2444402
version: 1
direction: direction/reverse
top level: 106GFP
start: 44
end: 918
orientation: inline

BBa_K1399023
type: ComponentDefinition
persistent identity: BBa K1399023
display id: BBa_K1399023
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ptet-GFP(DAS)
creator: Anna Stikane
created: 2014-09-18T11:00:00Z
modified: 2015-05-08T01:10:16Z
discontinued: false
dominant: false
group u list: _1777_
m user id: 0
owner id: 22477
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: Tetracycline/aTc inducible promoter with GFP reporter tagged with DAS-ssrA degradation tag followed by terminator. The tagged GFP is actively degraded within cell, thus provides better temporal resolution of green fluorescence and promoter activity.Presence of SspB is crucial for effective protein degradation.
mutable notes: Part was assembled using BrickClip assembly (BBF RFC104) using other biobrick parts as templates. BrickClip assembly is a special case of more general Paperclip assembly method.[1]
mutable provenance: other biobricks
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_B0034
component: BBa_R0040
component: BBa_K1399008
component: BBa_B0015
sequence annotation: BBa_B0015
sequence annotation: BBa_K1399008
sequence annotation: BBa_R0040
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K1399023_sequence/1

BBa_B0034
type: Component
persistent identity: component2384305
display id: component2384305
version: 1
top level: BBa_K1399023
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_R0040
type: Component
persistent identity: component2384299
display id: component2384299
version: 1
top level: BBa_K1399023
access: public
definition: https://synbiohub.org/public/igem/BBa_R0040/1

BBa_K1399008
type: Component
persistent identity: component2384311
display id: component2384311
version: 1
top level: BBa_K1399023
access: public
definition: BBa_K1399008

BBa_B0015
type: Component
persistent identity: component2384318
display id: component2384318
version: 1
top level: BBa_K1399023
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2384318
display id: annotation2384318
version: 1
top level: BBa_K1399023
location: https://synbiohub.org/public/igem/BBa_K1399023/annotation2384318/range2384318/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K1399023/annotation2384318/range2384318/1
type: Range
persistent identity: range2384318
display id: range2384318
version: 1
direction: direction/reverse
top level: BBa_K1399023
start: 842
end: 970
orientation: inline

BBa_K1399008
type: SequenceAnnotation
persistent identity: annotation2384311
display id: annotation2384311
version: 1
top level: BBa_K1399023
location: https://synbiohub.org/public/igem/BBa_K1399023/annotation2384311/range2384311/1
component: BBa_K1399008

https://synbiohub.org/public/igem/BBa_K1399023/annotation2384311/range2384311/1
type: Range
persistent identity: range2384311
display id: range2384311
version: 1
direction: direction/reverse
top level: BBa_K1399023
start: 81
end: 833
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2384299
display id: annotation2384299
version: 1
top level: BBa_K1399023
location: https://synbiohub.org/public/igem/BBa_K1399023/annotation2384299/range2384299/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_K1399023/annotation2384299/range2384299/1
type: Range
persistent identity: range2384299
display id: range2384299
version: 1
direction: direction/forward
top level: BBa_K1399023
start: 1
end: 54
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2384305
display id: annotation2384305
version: 1
top level: BBa_K1399023
location: https://synbiohub.org/public/igem/BBa_K1399023/annotation2384305/range2384305/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1399023/annotation2384305/range2384305/1
type: Range
persistent identity: range2384305
display id: range2384305
version: 1
direction: direction/reverse
top level: BBa_K1399023
start: 63
end: 74
orientation: inline

BBa_K1732024
type: ComponentDefinition
persistent identity: BBa K1732024
display id: BBa_K1732024
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: YFPCO-His
creator: Donna Lee
created: 2015-09-17T11:00:00Z
modified: 2016-01-21T02:27:14Z
discontinued: false
dominant: false
group u list: _2154_
m user id: 4206
owner id: 27195
owning group id: 9
status: status/Planning
bookmark: false
mutable description: YFP (BBa_K1491004) with 6X-His tag for easier protein purification. Sequence was codon optimized for E.coli via IDT codon optimizing tool.
mutable notes: Codon optimized for E.coli and 6X-His tag for easier protein purification.
mutable provenance: BBa_K1491004
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: 6X-His
sequence annotation: YFPCO
sequence: https://synbiohub.org/public/igem/BBa_K1732024_sequence/1

6X-His
type: SequenceAnnotation
persistent identity: annotation2469350
display id: annotation2469350
version: 1
top level: BBa_K1732024
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1732024/annotation2469350/range2469350/1

https://synbiohub.org/public/igem/BBa_K1732024/annotation2469350/range2469350/1
type: Range
persistent identity: range2469350
display id: range2469350
version: 1
direction: direction/forward
top level: BBa_K1732024
start: 717
end: 735
orientation: inline

YFPCO
type: SequenceAnnotation
persistent identity: annotation2469348
display id: annotation2469348
version: 1
top level: BBa_K1732024
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1732024/annotation2469348/range2469348/1

https://synbiohub.org/public/igem/BBa_K1732024/annotation2469348/range2469348/1
type: Range
persistent identity: range2469348
display id: range2469348
version: 1
direction: direction/forward
top level: BBa_K1732024
start: 1
end: 716
orientation: inline

ecfp
type: ComponentDefinition
persistent identity: BBa I15016
display id: BBa_I15016
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: B0032.ECFP
creator: Jeff Tabor
created: 2005-04-14T11:00:00Z
modified: 2015-08-31T04:07:38Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _5_
m user id: 0
owner id: 88
owning group id: 5
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This part accepts POPS to produce ECFP under the medium RBS B0032
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_E0020
component: BBa_B0032
sequence annotation: BBa_E0020
sequence annotation: BBa_B0032
sequence: https://synbiohub.org/public/igem/BBa_I15016_sequence/1

BBa_E0020
type: Component
persistent identity: component1478408
display id: component1478408
version: 1
top level: ecfp
access: public
definition: ecfp

BBa_B0032
type: Component
persistent identity: component1478405
display id: component1478405
version: 1
top level: ecfp
access: public
definition: https://synbiohub.org/public/igem/BBa_B0032/1

BBa_E0020
type: SequenceAnnotation
persistent identity: annotation1478408
display id: annotation1478408
version: 1
top level: ecfp
location: https://synbiohub.org/public/igem/BBa_I15016/annotation1478408/range1478408/1
component: BBa_E0020

https://synbiohub.org/public/igem/BBa_I15016/annotation1478408/range1478408/1
type: Range
persistent identity: range1478408
display id: range1478408
version: 1
direction: direction/forward
top level: ecfp
start: 20
end: 742
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation1478405
display id: annotation1478405
version: 1
top level: ecfp
location: https://synbiohub.org/public/igem/BBa_I15016/annotation1478405/range1478405/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_I15016/annotation1478405/range1478405/1
type: Range
persistent identity: range1478405
display id: range1478405
version: 1
direction: direction/forward
top level: ecfp
start: 1
end: 13
orientation: inline

GFP-GOI
type: ComponentDefinition
persistent identity: BBa K678028
display id: BBa_K678028
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: GFP-GOI
creator: DTU-Denmark-2
created: 2011-09-10T11:00:00Z
modified: 2015-05-08T01:13:04Z
discontinued: false
dominant: false
experience: experience/None
group u list: _882_
m user id: 0
owner id: 8341
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: GFP for Aspergillus nidulans
mutable notes: To be announced
mutable provenance: Design considerations This part was designed for the Plug'n'Play with DNA assembly standard (RFC 80), therefore this part contains linkers at the 5' and 3' ends as shown on the figure below.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: GFP
sequence annotation: Linker 2
sequence: https://synbiohub.org/public/igem/BBa_K678028_sequence/1

GFP
type: SequenceAnnotation
persistent identity: annotation2127385
display id: annotation2127385
version: 1
top level: GFP-GOI
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K678028/annotation2127385/range2127385/1

https://synbiohub.org/public/igem/BBa_K678028/annotation2127385/range2127385/1
type: Range
persistent identity: range2127385
display id: range2127385
version: 1
direction: direction/forward
top level: GFP-GOI
start: 9
end: 725
orientation: inline

Linker 2
type: SequenceAnnotation
persistent identity: annotation2127384
display id: annotation2127384
version: 1
top level: GFP-GOI
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678028/annotation2127384/range2127384/1

https://synbiohub.org/public/igem/BBa_K678028/annotation2127384/range2127384/1
type: Range
persistent identity: range2127384
display id: range2127384
version: 1
direction: direction/forward
top level: GFP-GOI
start: 1
end: 8
orientation: inline

BBa_K1680022
type: ComponentDefinition
persistent identity: BBa K1680022
display id: BBa_K1680022
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Dronpa caged Cre with NLS
creator: Nicolai von Kuegelgen
created: 2015-09-16T11:00:00Z
modified: 2015-10-27T01:52:38Z
discontinued: false
dominant: false
group u list: _2098_
m user id: 15693
owner id: 26366
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Protein coding region for a fusion protein of Cre recombinase (BBa_K1680007) that is C- & N-terminal flanked with 145N Dronpa (BBa_K1680006) using a 9 AA linker as spacer (BBa_K1680001). The construct has a N-terminal NLS sequence (BBa_K1680004). In the expressed construct the two dronpa domains should form multimerize leading to an inactive Cre recombinase that is reversibly activatable through dronpa illumination (see Zhou et al 2012).
mutable notes: 3A Assembly using RFC25
mutable provenance: gBlock designed by Team Tuebingen
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Linker
sequence annotation: Cre recombinase
sequence annotation: Dronpa
sequence annotation: SV40 NLS
sequence: https://synbiohub.org/public/igem/BBa_K1680022_sequence/1

Linker
type: SequenceAnnotation
persistent identity: annotation2477387
display id: annotation2477387
version: 1
top level: BBa_K1680022
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1680022/annotation2477387/range2477387/1

https://synbiohub.org/public/igem/BBa_K1680022/annotation2477387/range2477387/1
type: Range
persistent identity: range2477387
display id: range2477387
version: 1
direction: direction/forward
top level: BBa_K1680022
start: 700
end: 726
orientation: inline

Cre recombinase
type: SequenceAnnotation
persistent identity: annotation2477388
display id: annotation2477388
version: 1
top level: BBa_K1680022
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1680022/annotation2477388/range2477388/1

https://synbiohub.org/public/igem/BBa_K1680022/annotation2477388/range2477388/1
type: Range
persistent identity: range2477388
display id: range2477388
version: 1
direction: direction/forward
top level: BBa_K1680022
start: 727
end: 1755
orientation: inline

Dronpa
type: SequenceAnnotation
persistent identity: annotation2477386
display id: annotation2477386
version: 1
top level: BBa_K1680022
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1680022/annotation2477386/range2477386/1

https://synbiohub.org/public/igem/BBa_K1680022/annotation2477386/range2477386/1
type: Range
persistent identity: range2477386
display id: range2477386
version: 1
direction: direction/forward
top level: BBa_K1680022
start: 31
end: 699
orientation: inline

Linker
type: SequenceAnnotation
persistent identity: annotation2477389
display id: annotation2477389
version: 1
top level: BBa_K1680022
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1680022/annotation2477389/range2477389/1

https://synbiohub.org/public/igem/BBa_K1680022/annotation2477389/range2477389/1
type: Range
persistent identity: range2477389
display id: range2477389
version: 1
direction: direction/forward
top level: BBa_K1680022
start: 1756
end: 1783
orientation: inline

Dronpa
type: SequenceAnnotation
persistent identity: annotation2477391
display id: annotation2477391
version: 1
top level: BBa_K1680022
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1680022/annotation2477391/range2477391/1

https://synbiohub.org/public/igem/BBa_K1680022/annotation2477391/range2477391/1
type: Range
persistent identity: range2477391
display id: range2477391
version: 1
direction: direction/forward
top level: BBa_K1680022
start: 1783
end: 2451
orientation: inline

SV40 NLS
type: SequenceAnnotation
persistent identity: annotation2477385
display id: annotation2477385
version: 1
top level: BBa_K1680022
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1680022/annotation2477385/range2477385/1

https://synbiohub.org/public/igem/BBa_K1680022/annotation2477385/range2477385/1
type: Range
persistent identity: range2477385
display id: range2477385
version: 1
direction: direction/forward
top level: BBa_K1680022
start: 4
end: 24
orientation: inline

BBa_K1907008
type: ComponentDefinition
persistent identity: BBa K1907008
display id: BBa_K1907008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CCP1 promoter + Venus YFP
creator: Hele Haapaniemi
created: 2016-10-18T11:00:00Z
modified: 2016-10-19T03:08:57Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2373_
m user id: 29675
owner id: 29693
owning group id: 9
status: status/Planning
bookmark: false
mutable description: --
mutable notes: --
mutable provenance: --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1907005
component: BBa_K1907000
sequence annotation: BBa_K1907005
sequence annotation: BBa_K1907000
sequence: https://synbiohub.org/public/igem/BBa_K1907008_sequence/1

BBa_K1907005
type: Component
persistent identity: component2522670
display id: component2522670
version: 1
top level: BBa_K1907008
access: public
definition: https://synbiohub.org/public/igem/BBa_K1907005/1

BBa_K1907000
type: Component
persistent identity: component2522671
display id: component2522671
version: 1
top level: BBa_K1907008
access: public
definition: https://synbiohub.org/public/igem/BBa_K1907000/1

BBa_K1907005
type: SequenceAnnotation
persistent identity: annotation2522670
display id: annotation2522670
version: 1
top level: BBa_K1907008
location: https://synbiohub.org/public/igem/BBa_K1907008/annotation2522670/range2522670/1
component: BBa_K1907005

https://synbiohub.org/public/igem/BBa_K1907008/annotation2522670/range2522670/1
type: Range
persistent identity: range2522670
display id: range2522670
version: 1
direction: direction/reverse
top level: BBa_K1907008
start: 1
end: 756
orientation: inline

BBa_K1907000
type: SequenceAnnotation
persistent identity: annotation2522671
display id: annotation2522671
version: 1
top level: BBa_K1907008
location: https://synbiohub.org/public/igem/BBa_K1907008/annotation2522671/range2522671/1
component: BBa_K1907000

https://synbiohub.org/public/igem/BBa_K1907008/annotation2522671/range2522671/1
type: Range
persistent identity: range2522671
display id: range2522671
version: 1
direction: direction/reverse
top level: BBa_K1907008
start: 763
end: 1479
orientation: inline

BBa_K546547
type: ComponentDefinition
persistent identity: BBa K546547
display id: BBa_K546547
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Constitutive (tetR repressible) LacI and RFP expression
creator: Brendan Ryback, Matthijn Hesselman
created: 2011-09-15T11:00:00Z
modified: 2015-05-08T01:12:39Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _713_
m user id: 0
owner id: 8616
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part constitutively expresses LacI and RFP except when repressed by TetR. When cells containing this part are exposed to the inducer molecule IPTG, it can be used to regulate lac-repressible promoters. It was designed to function as an IPTG-controllable tuner for the [http://partsregistry.org/Part:BBa_K546546 Double Tunable Synchronized Oscillator]. It is advisable to operate this part on a low or medium copy plasmid.
mutable notes: No specific considerations.
mutable provenance: Composite part of R0040 and J24675
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
component: BBa_R0040
component: BBa_J24675
sequence annotation: BBa_J24675
sequence annotation: BBa_R0040
sequence: https://synbiohub.org/public/igem/BBa_K546547_sequence/1

BBa_R0040
type: Component
persistent identity: component2268506
display id: component2268506
version: 1
top level: BBa_K546547
access: public
definition: https://synbiohub.org/public/igem/BBa_R0040/1

BBa_J24675
type: Component
persistent identity: component2268530
display id: component2268530
version: 1
top level: BBa_K546547
access: public
definition: https://synbiohub.org/public/igem/BBa_J24675/1

BBa_J24675
type: SequenceAnnotation
persistent identity: annotation2268530
display id: annotation2268530
version: 1
top level: BBa_K546547
location: https://synbiohub.org/public/igem/BBa_K546547/annotation2268530/range2268530/1
component: BBa_J24675

https://synbiohub.org/public/igem/BBa_K546547/annotation2268530/range2268530/1
type: Range
persistent identity: range2268530
display id: range2268530
version: 1
direction: direction/reverse
top level: BBa_K546547
start: 63
end: 2110
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2268506
display id: annotation2268506
version: 1
top level: BBa_K546547
location: https://synbiohub.org/public/igem/BBa_K546547/annotation2268506/range2268506/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_K546547/annotation2268506/range2268506/1
type: Range
persistent identity: range2268506
display id: range2268506
version: 1
direction: direction/forward
top level: BBa_K546547
start: 1
end: 54
orientation: inline

SgsE | YFP
type: ComponentDefinition
persistent identity: BBa K525305
display id: BBa_K525305
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Fusion Protein of S-Layer SgsE and mCitrine
creator: Timo Wolf
created: 2011-09-09T11:00:00Z
modified: 2016-01-26T01:30:31Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _690_
m user id: 4206
owner id: 6668
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: Fusion Protein of S-Layer SgsE and mCitrine
mutable notes: Fusion Protein of BBa_K525303 and BBa_J18931
mutable provenance: Fusion Protein of S-Layer SgsE and mCitrine
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: SgsE domain
sequence annotation: ATG
sequence annotation: mCitrine (YFP)
sequence annotation: K525301
sequence annotation: B0034
sequence annotation: AgeI
sequence annotation: J18931
sequence annotation: RBS
sequence annotation: T7 promoter
sequence annotation: NgoMIV
sequence annotation: lac operator
sequence annotation: TAA
sequence annotation: Freiburg scar
sequence: https://synbiohub.org/public/igem/BBa_K525305_sequence/1

SgsE domain
type: SequenceAnnotation
persistent identity: annotation2127334
display id: annotation2127334
version: 1
top level: SgsE | YFP
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K525305/annotation2127334/range2127334/1

https://synbiohub.org/public/igem/BBa_K525305/annotation2127334/range2127334/1
type: Range
persistent identity: range2127334
display id: range2127334
version: 1
direction: direction/forward
top level: SgsE | YFP
start: 73
end: 2400
orientation: inline

ATG
type: SequenceAnnotation
persistent identity: annotation2127333
display id: annotation2127333
version: 1
top level: SgsE | YFP
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K525305/annotation2127333/range2127333/1

https://synbiohub.org/public/igem/BBa_K525305/annotation2127333/range2127333/1
type: Range
persistent identity: range2127333
display id: range2127333
version: 1
direction: direction/forward
top level: SgsE | YFP
start: 73
end: 75
orientation: inline

mCitrine (YFP)
type: SequenceAnnotation
persistent identity: annotation2127336
display id: annotation2127336
version: 1
top level: SgsE | YFP
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K525305/annotation2127336/range2127336/1

https://synbiohub.org/public/igem/BBa_K525305/annotation2127336/range2127336/1
type: Range
persistent identity: range2127336
display id: range2127336
version: 1
direction: direction/forward
top level: SgsE | YFP
start: 2407
end: 3129
orientation: inline

K525301
type: SequenceAnnotation
persistent identity: annotation2127339
display id: annotation2127339
version: 1
top level: SgsE | YFP
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K525305/annotation2127339/range2127339/1

https://synbiohub.org/public/igem/BBa_K525305/annotation2127339/range2127339/1
type: Range
persistent identity: range2127339
display id: range2127339
version: 1
direction: direction/forward
top level: SgsE | YFP
start: 73
end: 2400
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation2127331
display id: annotation2127331
version: 1
top level: SgsE | YFP
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K525305/annotation2127331/range2127331/1

https://synbiohub.org/public/igem/BBa_K525305/annotation2127331/range2127331/1
type: Range
persistent identity: range2127331
display id: range2127331
version: 1
direction: direction/forward
top level: SgsE | YFP
start: 53
end: 64
orientation: inline

AgeI
type: SequenceAnnotation
persistent identity: annotation2127338
display id: annotation2127338
version: 1
top level: SgsE | YFP
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K525305/annotation2127338/range2127338/1

https://synbiohub.org/public/igem/BBa_K525305/annotation2127338/range2127338/1
type: Range
persistent identity: range2127338
display id: range2127338
version: 1
direction: direction/forward
top level: SgsE | YFP
start: 3121
end: 3126
orientation: inline

J18931
type: SequenceAnnotation
persistent identity: annotation2127341
display id: annotation2127341
version: 1
top level: SgsE | YFP
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K525305/annotation2127341/range2127341/1

https://synbiohub.org/public/igem/BBa_K525305/annotation2127341/range2127341/1
type: Range
persistent identity: range2127341
display id: range2127341
version: 1
direction: direction/forward
top level: SgsE | YFP
start: 2407
end: 3129
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2127330
display id: annotation2127330
version: 1
top level: SgsE | YFP
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K525305/annotation2127330/range2127330/1

https://synbiohub.org/public/igem/BBa_K525305/annotation2127330/range2127330/1
type: Range
persistent identity: range2127330
display id: range2127330
version: 1
direction: direction/forward
top level: SgsE | YFP
start: 56
end: 59
orientation: inline

T7 promoter
type: SequenceAnnotation
persistent identity: annotation2127327
display id: annotation2127327
version: 1
top level: SgsE | YFP
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K525305/annotation2127327/range2127327/1

https://synbiohub.org/public/igem/BBa_K525305/annotation2127327/range2127327/1
type: Range
persistent identity: range2127327
display id: range2127327
version: 1
direction: direction/forward
top level: SgsE | YFP
start: 1
end: 20
orientation: inline

NgoMIV
type: SequenceAnnotation
persistent identity: annotation2127332
display id: annotation2127332
version: 1
top level: SgsE | YFP
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K525305/annotation2127332/range2127332/1

https://synbiohub.org/public/igem/BBa_K525305/annotation2127332/range2127332/1
type: Range
persistent identity: range2127332
display id: range2127332
version: 1
direction: direction/forward
top level: SgsE | YFP
start: 76
end: 81
orientation: inline

lac operator
type: SequenceAnnotation
persistent identity: annotation2127328
display id: annotation2127328
version: 1
top level: SgsE | YFP
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K525305/annotation2127328/range2127328/1

https://synbiohub.org/public/igem/BBa_K525305/annotation2127328/range2127328/1
type: Range
persistent identity: range2127328
display id: range2127328
version: 1
direction: direction/forward
top level: SgsE | YFP
start: 20
end: 44
orientation: inline

TAA
type: SequenceAnnotation
persistent identity: annotation2127337
display id: annotation2127337
version: 1
top level: SgsE | YFP
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K525305/annotation2127337/range2127337/1

https://synbiohub.org/public/igem/BBa_K525305/annotation2127337/range2127337/1
type: Range
persistent identity: range2127337
display id: range2127337
version: 1
direction: direction/forward
top level: SgsE | YFP
start: 3127
end: 3129
orientation: inline

Freiburg scar
type: SequenceAnnotation
persistent identity: annotation2127335
display id: annotation2127335
version: 1
top level: SgsE | YFP
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K525305/annotation2127335/range2127335/1

https://synbiohub.org/public/igem/BBa_K525305/annotation2127335/range2127335/1
type: Range
persistent identity: range2127335
display id: range2127335
version: 1
direction: direction/forward
top level: SgsE | YFP
start: 2401
end: 2406
orientation: inline

SbpA | YFP
type: ComponentDefinition
persistent identity: BBa K525405
display id: BBa_K525405
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Fusion Protein of S-Layer SbpA and mCitrine
creator: Timo Wolf
created: 2011-09-09T11:00:00Z
modified: 2016-01-26T10:05:40Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _690_
m user id: 4206
owner id: 6668
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Fusion Protein of S-Layer sbpA and mCitrine
mutable notes: Fusion Protein of BBa_K525403 and BBa_J18931
mutable provenance: Fusion Protein of S-Layer sbpA and mCitrine
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: T7 promoter
sequence annotation: SbpA domain
sequence annotation: lac operator
sequence annotation: AgeI
sequence annotation: RBS
sequence annotation: TAA
sequence annotation: NgoMIV
sequence annotation: B0034
sequence annotation: Freiburg scar
sequence annotation: J18931
sequence annotation: ATG
sequence annotation: K525401
sequence annotation: mCitrine (YFP)
sequence: https://synbiohub.org/public/igem/BBa_K525405_sequence/1

T7 promoter
type: SequenceAnnotation
persistent identity: annotation2127654
display id: annotation2127654
version: 1
top level: SbpA | YFP
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K525405/annotation2127654/range2127654/1

https://synbiohub.org/public/igem/BBa_K525405/annotation2127654/range2127654/1
type: Range
persistent identity: range2127654
display id: range2127654
version: 1
direction: direction/forward
top level: SbpA | YFP
start: 1
end: 20
orientation: inline

SbpA domain
type: SequenceAnnotation
persistent identity: annotation2127664
display id: annotation2127664
version: 1
top level: SbpA | YFP
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K525405/annotation2127664/range2127664/1

https://synbiohub.org/public/igem/BBa_K525405/annotation2127664/range2127664/1
type: Range
persistent identity: range2127664
display id: range2127664
version: 1
direction: direction/forward
top level: SbpA | YFP
start: 73
end: 3192
orientation: inline

lac operator
type: SequenceAnnotation
persistent identity: annotation2127655
display id: annotation2127655
version: 1
top level: SbpA | YFP
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K525405/annotation2127655/range2127655/1

https://synbiohub.org/public/igem/BBa_K525405/annotation2127655/range2127655/1
type: Range
persistent identity: range2127655
display id: range2127655
version: 1
direction: direction/forward
top level: SbpA | YFP
start: 20
end: 44
orientation: inline

AgeI
type: SequenceAnnotation
persistent identity: annotation2127662
display id: annotation2127662
version: 1
top level: SbpA | YFP
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K525405/annotation2127662/range2127662/1

https://synbiohub.org/public/igem/BBa_K525405/annotation2127662/range2127662/1
type: Range
persistent identity: range2127662
display id: range2127662
version: 1
direction: direction/forward
top level: SbpA | YFP
start: 3913
end: 3918
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2127657
display id: annotation2127657
version: 1
top level: SbpA | YFP
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K525405/annotation2127657/range2127657/1

https://synbiohub.org/public/igem/BBa_K525405/annotation2127657/range2127657/1
type: Range
persistent identity: range2127657
display id: range2127657
version: 1
direction: direction/forward
top level: SbpA | YFP
start: 56
end: 59
orientation: inline

TAA
type: SequenceAnnotation
persistent identity: annotation2127661
display id: annotation2127661
version: 1
top level: SbpA | YFP
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K525405/annotation2127661/range2127661/1

https://synbiohub.org/public/igem/BBa_K525405/annotation2127661/range2127661/1
type: Range
persistent identity: range2127661
display id: range2127661
version: 1
direction: direction/forward
top level: SbpA | YFP
start: 3919
end: 3921
orientation: inline

NgoMIV
type: SequenceAnnotation
persistent identity: annotation2127660
display id: annotation2127660
version: 1
top level: SbpA | YFP
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K525405/annotation2127660/range2127660/1

https://synbiohub.org/public/igem/BBa_K525405/annotation2127660/range2127660/1
type: Range
persistent identity: range2127660
display id: range2127660
version: 1
direction: direction/forward
top level: SbpA | YFP
start: 76
end: 81
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation2127656
display id: annotation2127656
version: 1
top level: SbpA | YFP
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K525405/annotation2127656/range2127656/1

https://synbiohub.org/public/igem/BBa_K525405/annotation2127656/range2127656/1
type: Range
persistent identity: range2127656
display id: range2127656
version: 1
direction: direction/forward
top level: SbpA | YFP
start: 53
end: 64
orientation: inline

Freiburg scar
type: SequenceAnnotation
persistent identity: annotation2127665
display id: annotation2127665
version: 1
top level: SbpA | YFP
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K525405/annotation2127665/range2127665/1

https://synbiohub.org/public/igem/BBa_K525405/annotation2127665/range2127665/1
type: Range
persistent identity: range2127665
display id: range2127665
version: 1
direction: direction/forward
top level: SbpA | YFP
start: 3193
end: 3198
orientation: inline

J18931
type: SequenceAnnotation
persistent identity: annotation2127667
display id: annotation2127667
version: 1
top level: SbpA | YFP
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K525405/annotation2127667/range2127667/1

https://synbiohub.org/public/igem/BBa_K525405/annotation2127667/range2127667/1
type: Range
persistent identity: range2127667
display id: range2127667
version: 1
direction: direction/forward
top level: SbpA | YFP
start: 3199
end: 3921
orientation: inline

ATG
type: SequenceAnnotation
persistent identity: annotation2127658
display id: annotation2127658
version: 1
top level: SbpA | YFP
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K525405/annotation2127658/range2127658/1

https://synbiohub.org/public/igem/BBa_K525405/annotation2127658/range2127658/1
type: Range
persistent identity: range2127658
display id: range2127658
version: 1
direction: direction/forward
top level: SbpA | YFP
start: 73
end: 75
orientation: inline

K525401
type: SequenceAnnotation
persistent identity: annotation2127663
display id: annotation2127663
version: 1
top level: SbpA | YFP
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K525405/annotation2127663/range2127663/1

https://synbiohub.org/public/igem/BBa_K525405/annotation2127663/range2127663/1
type: Range
persistent identity: range2127663
display id: range2127663
version: 1
direction: direction/forward
top level: SbpA | YFP
start: 73
end: 3192
orientation: inline

mCitrine (YFP)
type: SequenceAnnotation
persistent identity: annotation2127666
display id: annotation2127666
version: 1
top level: SbpA | YFP
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K525405/annotation2127666/range2127666/1

https://synbiohub.org/public/igem/BBa_K525405/annotation2127666/range2127666/1
type: Range
persistent identity: range2127666
display id: range2127666
version: 1
direction: direction/forward
top level: SbpA | YFP
start: 3199
end: 3921
orientation: inline

BBa_K1789004
type: ComponentDefinition
persistent identity: BBa K1789004
display id: BBa_K1789004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: GFP2
creator: Xinyuan Qiu
created: 2015-09-13T11:00:00Z
modified: 2016-01-25T01:04:48Z
discontinued: false
dominant: false
group u list: _2214_
m user id: 4206
owner id: 21134
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This part is the Carboxyl Half of GFP
mutable notes: we REMOVED the extra base from part BBa_I715020(easier to fuse with other proteins) when designing PCR primers to make the protein fusion easier.
mutable provenance: This part is gained from part BBa_I715020.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: GFP2
sequence: https://synbiohub.org/public/igem/BBa_K1789004_sequence/1

GFP2
type: SequenceAnnotation
persistent identity: annotation2454067
display id: annotation2454067
version: 1
top level: BBa_K1789004
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1789004/annotation2454067/range2454067/1

https://synbiohub.org/public/igem/BBa_K1789004/annotation2454067/range2454067/1
type: Range
persistent identity: range2454067
display id: range2454067
version: 1
direction: direction/forward
top level: BBa_K1789004
start: 1
end: 249
orientation: inline

ecfp
type: ComponentDefinition
persistent identity: BBa E0020
display id: BBa_E0020
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: engineered cyan fluorescent protein derived from A. victoria GFP
creator: Caitlin Conboy and Jennifer Braff
created: 2004-03-02T12:00:00Z
modified: 2015-08-31T04:07:25Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_E0020_sequence/1

BBa_K1510017
type: ComponentDefinition
persistent identity: BBa K1510017
display id: BBa_K1510017
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: red florescence protein RFC23
creator: Chien-Yung Huang
created: 2014-10-05T11:00:00Z
modified: 2016-01-26T02:09:57Z
discontinued: false
dominant: false
group u list: _1890_
m user id: 0
m user id: 4206
owner id: 21268
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: The part is basically a sequence encoding red florescent protein. With specially designed primers, it can be fused with its previous signal protein, yebF.
mutable notes: It is RFC23 compatible, with specially designed prefix.
mutable provenance: Obtaining this sequence, we designed primers to take the sequence from IGEM distributed part, BBa_E1010.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1510017_sequence/1

BBa_K621001
type: ComponentDefinition
persistent identity: BBa K621001
display id: BBa_K621001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: OMP-R-->RBS-->eYFP
creator: David Pohlman, Blythe Ferriere, Aile Able, Erica McFarland
created: 2011-09-26T11:00:00Z
modified: 2015-05-08T01:12:55Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _793_
m user id: 0
owner id: 4838
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: Our part consists of an OMP-R binding region that regulates our reporter gene, eYFP. The OMP-R binding region is an omp-c operator, which has three OMP-R binding sites. Our part is regulated by the ENVZ-OMPR two-component regulatory system. ENVZ is a osmolarity sensor on the inner membrane and phosphorylates OMP-R in the presence of high osmolarity. When phosphorylated, OMP-R binds to the omp-c operator and recruits RNAP to transcribe the downstream reporter gene. Fluorescence intensity due to the production of eYFP is a measure of the system's activity. When all three binding sites are occupied by phosphorylated OMP-R proteins, RNAP cannot bind to the DNA and transcribe downstream. This part can be used as an osmolarity indicator. Our team used this part as a glucose sensor because the presence of glucose changes osmolarity. Different concentrations of glucose produce different, quantifiable fluorescence intensities.
mutable notes: Our team had to combine the genes in a specific order. First our team cut out and purified the eYPF gene. We added this gene to a plasmid containing RBS. Next we cut out the RBS+eYFP intermediate and ligated them into the plasmid containing OMP-R. The RBS and OMP-R parts were too small to run out and extract on a gel because they would run off of the end of the gel. Therefore, we had to start the process with eYPF.
mutable provenance: This BioBrick was created from parts found in the Registry. These parts were BBa_R0082, BBa_B0032, and BBa_E0030.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_E0030
component: BBa_B0032
component: BBa_R0082
sequence annotation: BBa_R0082
sequence annotation: BBa_B0032
sequence annotation: BBa_E0030
sequence: https://synbiohub.org/public/igem/BBa_K621001_sequence/1

BBa_E0030
type: Component
persistent identity: component2145049
display id: component2145049
version: 1
top level: BBa_K621001
access: public
definition: eyfp

BBa_B0032
type: Component
persistent identity: component2145047
display id: component2145047
version: 1
top level: BBa_K621001
access: public
definition: https://synbiohub.org/public/igem/BBa_B0032/1

BBa_R0082
type: Component
persistent identity: component2145045
display id: component2145045
version: 1
top level: BBa_K621001
access: public
definition: https://synbiohub.org/public/igem/BBa_R0082/1

BBa_R0082
type: SequenceAnnotation
persistent identity: annotation2145045
display id: annotation2145045
version: 1
top level: BBa_K621001
location: https://synbiohub.org/public/igem/BBa_K621001/annotation2145045/range2145045/1
component: BBa_R0082

https://synbiohub.org/public/igem/BBa_K621001/annotation2145045/range2145045/1
type: Range
persistent identity: range2145045
display id: range2145045
version: 1
direction: direction/reverse
top level: BBa_K621001
start: 1
end: 108
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation2145047
display id: annotation2145047
version: 1
top level: BBa_K621001
location: https://synbiohub.org/public/igem/BBa_K621001/annotation2145047/range2145047/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_K621001/annotation2145047/range2145047/1
type: Range
persistent identity: range2145047
display id: range2145047
version: 1
direction: direction/forward
top level: BBa_K621001
start: 117
end: 129
orientation: inline

BBa_E0030
type: SequenceAnnotation
persistent identity: annotation2145049
display id: annotation2145049
version: 1
top level: BBa_K621001
location: https://synbiohub.org/public/igem/BBa_K621001/annotation2145049/range2145049/1
component: BBa_E0030

https://synbiohub.org/public/igem/BBa_K621001/annotation2145049/range2145049/1
type: Range
persistent identity: range2145049
display id: range2145049
version: 1
direction: direction/reverse
top level: BBa_K621001
start: 136
end: 858
orientation: inline

mRFP1
type: ComponentDefinition
persistent identity: BBa I13503
display id: BBa_I13503
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Screening plasmid intermediate
creator: jkm
created: 2005-05-30T11:00:00Z
modified: 2015-08-31T04:07:34Z
discontinued: false
dominant: true
experience: experience/None
group u list: _11_
m user id: 0
owner id: 253
owning group id: 6
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Built by Josh as an intermediate in screening plasmid construction
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_B0032
component: BBa_E1010
sequence annotation: BBa_E1010
sequence annotation: BBa_B0032
sequence: https://synbiohub.org/public/igem/BBa_I13503_sequence/1

BBa_B0032
type: Component
persistent identity: component1505053
display id: component1505053
version: 1
top level: mRFP1
access: public
definition: https://synbiohub.org/public/igem/BBa_B0032/1

BBa_E1010
type: Component
persistent identity: component1505061
display id: component1505061
version: 1
top level: mRFP1
access: public
definition: mRFP1

BBa_E1010
type: SequenceAnnotation
persistent identity: annotation1505061
display id: annotation1505061
version: 1
top level: mRFP1
location: https://synbiohub.org/public/igem/BBa_I13503/annotation1505061/range1505061/1
component: BBa_E1010

https://synbiohub.org/public/igem/BBa_I13503/annotation1505061/range1505061/1
type: Range
persistent identity: range1505061
display id: range1505061
version: 1
direction: direction/forward
top level: mRFP1
start: 20
end: 700
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation1505053
display id: annotation1505053
version: 1
top level: mRFP1
location: https://synbiohub.org/public/igem/BBa_I13503/annotation1505053/range1505053/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_I13503/annotation1505053/range1505053/1
type: Range
persistent identity: range1505053
display id: range1505053
version: 1
direction: direction/forward
top level: mRFP1
start: 1
end: 13
orientation: inline

RFP1
type: ComponentDefinition
persistent identity: BBa I715022
display id: BBa_I715022
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Amino Portion of RFP
creator: Andrew Martens
created: 2007-06-24T11:00:00Z
modified: 2016-01-25T04:43:58Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _120_
m user id: 4206
owner id: 201
owning group id: 61
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: af
mutable notes: afd
mutable provenance: adf
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Coding sequence
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_I715022_sequence/1

Coding sequence
type: SequenceAnnotation
persistent identity: annotation1935381
display id: annotation1935381
version: 1
top level: RFP1
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_I715022/annotation1935381/range1935381/1

https://synbiohub.org/public/igem/BBa_I715022/annotation1935381/range1935381/1
type: Range
persistent identity: range1935381
display id: range1935381
version: 1
direction: direction/forward
top level: RFP1
start: 1
end: 462
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation1935380
display id: annotation1935380
version: 1
top level: RFP1
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_I715022/annotation1935380/range1935380/1

https://synbiohub.org/public/igem/BBa_I715022/annotation1935380/range1935380/1
type: Range
persistent identity: range1935380
display id: range1935380
version: 1
direction: direction/forward
top level: RFP1
start: 1
end: 3
orientation: inline

BBa_K1031420
type: ComponentDefinition
persistent identity: BBa K1031420
display id: BBa_K1031420
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ph-B0031-sfGFP-Terminator (HcaR)
creator: He Shuaixin
created: 2013-09-08T11:00:00Z
modified: 2015-05-08T01:08:47Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1338_
m user id: 0
owner id: 15843
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Ph-sfGFP-TT (HcaR)
mutable notes: molecular cloning
mutable provenance: synthesis, lab made
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: Ph (HcaR)
sequence annotation: B0031
sequence annotation: B0015
sequence annotation: sfGFP
sequence: https://synbiohub.org/public/igem/BBa_K1031420_sequence/1

Ph (HcaR)
type: SequenceAnnotation
persistent identity: annotation2362324
display id: annotation2362324
version: 1
top level: BBa_K1031420
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1031420/annotation2362324/range2362324/1

https://synbiohub.org/public/igem/BBa_K1031420/annotation2362324/range2362324/1
type: Range
persistent identity: range2362324
display id: range2362324
version: 1
direction: direction/forward
top level: BBa_K1031420
start: 1
end: 74
orientation: inline

B0031
type: SequenceAnnotation
persistent identity: annotation2362325
display id: annotation2362325
version: 1
top level: BBa_K1031420
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1031420/annotation2362325/range2362325/1

https://synbiohub.org/public/igem/BBa_K1031420/annotation2362325/range2362325/1
type: Range
persistent identity: range2362325
display id: range2362325
version: 1
direction: direction/forward
top level: BBa_K1031420
start: 81
end: 94
orientation: inline

B0015
type: SequenceAnnotation
persistent identity: annotation2362327
display id: annotation2362327
version: 1
top level: BBa_K1031420
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1031420/annotation2362327/range2362327/1

https://synbiohub.org/public/igem/BBa_K1031420/annotation2362327/range2362327/1
type: Range
persistent identity: range2362327
display id: range2362327
version: 1
direction: direction/forward
top level: BBa_K1031420
start: 830
end: 958
orientation: inline

sfGFP
type: SequenceAnnotation
persistent identity: annotation2362326
display id: annotation2362326
version: 1
top level: BBa_K1031420
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1031420/annotation2362326/range2362326/1

https://synbiohub.org/public/igem/BBa_K1031420/annotation2362326/range2362326/1
type: Range
persistent identity: range2362326
display id: range2362326
version: 1
direction: direction/forward
top level: BBa_K1031420
start: 101
end: 823
orientation: inline

BBa_K1185002
type: ComponentDefinition
persistent identity: BBa K1185002
display id: BBa_K1185002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: HBsu-RFP
creator: Vincent Leonardo
created: 2013-08-28T11:00:00Z
modified: 2015-06-08T09:30:41Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1498_
m user id: 4206
owner id: 16794
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This BioBrick codes for a HBsu protein attached to a red fluorescent protein (RFP). HBsu is a non-specific DNA binding protein that binds to DNA as a homodimer. The HBsu is joined to the RFP through ten amino acid flexible linker sequence. This allows the observation of ''Bacillus subtilis'' DNA using fluorescence microscopy. The integration strategy that we opted for was to clone in the BioBrick into the Multiple cloning site of pMutin4 backbone. First we attached HindIII restriction sites on 5'end and SacII restriction sites on 3'end of the BioBrick. We then cut the pMutin4 backbone and BioBrick using the previously mentioned restriction enzymes, this allowed us to ligate the plasmid and BioBrick together. We also attached a ~300bp amyE homology region onto the 3'end of the BioBrick after the SacII to allow the single cross over and integration of the whole plasmid into the genome. The pMutin4 plasmid contains a ery+ resistance marker for ''B.subtilis'' and amp+ for ''E.coli'' and also contains lacI, lacZ and Pspac promoter which is an IPTG induced promoter which regulated the transcription of this BioBrick. An alternative method to use this part would be to clone this BioBrick out and use any Assembly protocol to attach a desired promoter, RBS and antibiotic resistance genes.
mutable notes: None
mutable provenance: The source of the HBsu coding sequence was the ''B. subtilis'' strain 168 genome, SwissProtTM accession number: P08821. The RFP sequence was sourced from BBa_E1010 and the linker from: BBa_K105012.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: RBS-with post spacer
sequence annotation: RFP_BBa_E1010
sequence annotation: 3-frame stop codon
sequence annotation: hbs
sequence annotation: linker sequence_BBa_K105012
sequence: https://synbiohub.org/public/igem/BBa_K1185002_sequence/1

RBS-with post spacer
type: SequenceAnnotation
persistent identity: annotation2333320
display id: annotation2333320
version: 1
top level: BBa_K1185002
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1185002/annotation2333320/range2333320/1

https://synbiohub.org/public/igem/BBa_K1185002/annotation2333320/range2333320/1
type: Range
persistent identity: range2333320
display id: range2333320
version: 1
direction: direction/forward
top level: BBa_K1185002
start: 1
end: 20
orientation: inline

RFP_BBa_E1010
type: SequenceAnnotation
persistent identity: annotation2333324
display id: annotation2333324
version: 1
top level: BBa_K1185002
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1185002/annotation2333324/range2333324/1

https://synbiohub.org/public/igem/BBa_K1185002/annotation2333324/range2333324/1
type: Range
persistent identity: range2333324
display id: range2333324
version: 1
direction: direction/forward
top level: BBa_K1185002
start: 327
end: 1004
orientation: inline

3-frame stop codon
type: SequenceAnnotation
persistent identity: annotation2333326
display id: annotation2333326
version: 1
top level: BBa_K1185002
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1185002/annotation2333326/range2333326/1

https://synbiohub.org/public/igem/BBa_K1185002/annotation2333326/range2333326/1
type: Range
persistent identity: range2333326
display id: range2333326
version: 1
direction: direction/forward
top level: BBa_K1185002
start: 1005
end: 1012
orientation: inline

hbs
type: SequenceAnnotation
persistent identity: annotation2333321
display id: annotation2333321
version: 1
top level: BBa_K1185002
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1185002/annotation2333321/range2333321/1

https://synbiohub.org/public/igem/BBa_K1185002/annotation2333321/range2333321/1
type: Range
persistent identity: range2333321
display id: range2333321
version: 1
direction: direction/forward
top level: BBa_K1185002
start: 21
end: 296
orientation: inline

linker sequence_BBa_K105012
type: SequenceAnnotation
persistent identity: annotation2333322
display id: annotation2333322
version: 1
top level: BBa_K1185002
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1185002/annotation2333322/range2333322/1

https://synbiohub.org/public/igem/BBa_K1185002/annotation2333322/range2333322/1
type: Range
persistent identity: range2333322
display id: range2333322
version: 1
direction: direction/forward
top level: BBa_K1185002
start: 297
end: 326
orientation: inline

eyfp
type: ComponentDefinition
persistent identity: BBa I15020
display id: BBa_I15020
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: B0031.EYFP
creator: Jeff Tabor
created: 2005-04-14T11:00:00Z
modified: 2015-08-31T04:07:39Z
discontinued: false
dominant: true
experience: experience/None
group u list: _5_
m user id: 0
owner id: 88
owning group id: 5
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part accepts POPS to produce EYFP under the weak RBS B0031
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_E0030
component: BBa_B0031
sequence annotation: BBa_B0031
sequence annotation: BBa_E0030
sequence: https://synbiohub.org/public/igem/BBa_I15020_sequence/1

BBa_E0030
type: Component
persistent identity: component1478462
display id: component1478462
version: 1
top level: eyfp
access: public
definition: eyfp

BBa_B0031
type: Component
persistent identity: component1478460
display id: component1478460
version: 1
top level: eyfp
access: public
definition: https://synbiohub.org/public/igem/BBa_B0031/1

BBa_B0031
type: SequenceAnnotation
persistent identity: annotation1478460
display id: annotation1478460
version: 1
top level: eyfp
location: https://synbiohub.org/public/igem/BBa_I15020/annotation1478460/range1478460/1
component: BBa_B0031

https://synbiohub.org/public/igem/BBa_I15020/annotation1478460/range1478460/1
type: Range
persistent identity: range1478460
display id: range1478460
version: 1
direction: direction/forward
top level: eyfp
start: 1
end: 14
orientation: inline

BBa_E0030
type: SequenceAnnotation
persistent identity: annotation1478462
display id: annotation1478462
version: 1
top level: eyfp
location: https://synbiohub.org/public/igem/BBa_I15020/annotation1478462/range1478462/1
component: BBa_E0030

https://synbiohub.org/public/igem/BBa_I15020/annotation1478462/range1478462/1
type: Range
persistent identity: range1478462
display id: range1478462
version: 1
direction: direction/forward
top level: eyfp
start: 21
end: 743
orientation: inline

mCherryC
type: ComponentDefinition
persistent identity: BBa K1093017
display id: BBa_K1093017
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: mCherry C-terminal
creator: Anna Miścicka
created: 2013-09-20T11:00:00Z
modified: 2016-01-26T03:07:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1403_
m user id: 4206
owner id: 18431
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This is N-terminal of mCherry, that can be used in Bimolecular Fluorescence Complementation system (BiFC).
mutable notes: Created by PCR on mCherry.
mutable provenance: PartsRegistry J06505, originally from Discosoma sp.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: mCherry C-terminal
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_K1093017_sequence/1

mCherry C-terminal
type: SequenceAnnotation
persistent identity: annotation2357377
display id: annotation2357377
version: 1
top level: mCherryC
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1093017/annotation2357377/range2357377/1

https://synbiohub.org/public/igem/BBa_K1093017/annotation2357377/range2357377/1
type: Range
persistent identity: range2357377
display id: range2357377
version: 1
direction: direction/forward
top level: mCherryC
start: 1
end: 239
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2357376
display id: annotation2357376
version: 1
top level: mCherryC
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1093017/annotation2357376/range2357376/1

https://synbiohub.org/public/igem/BBa_K1093017/annotation2357376/range2357376/1
type: Range
persistent identity: range2357376
display id: range2357376
version: 1
direction: direction/forward
top level: mCherryC
start: 240
end: 243
orientation: inline

BBa_K1799026
type: ComponentDefinition
persistent identity: BBa K1799026
display id: BBa_K1799026
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Discosoma RFP with stp
creator: Ellen Jorgensen
created: 2015-09-17T11:00:00Z
modified: 2016-01-21T02:18:57Z
discontinued: false
dominant: false
group u list: _2224_
m user id: 4206
owner id: 9151
owning group id: 9
status: status/Planning
bookmark: false
mutable description: BBa_E1010 Discosoma RFP with stp
mutable notes: BBa_E1010 Discosoma RFP with stp
mutable provenance: BBa_E1010
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1799026_sequence/1

BBa_K191005
type: ComponentDefinition
persistent identity: BBa K191005
display id: BBa_K191005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TRP promoter - RBS - TetR - Term - TetR promoter - RBS - RFP - Term
creator: Le Thanh Tu NGUYEN
created: 2009-10-10T11:00:00Z
modified: 2015-05-08T01:11:15Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _287_
m user id: 0
owner id: 4198
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: TRP inhibits TetR's transcription while TetR inhibits RFP's transcription.
mutable notes: 1.5 step- PCR was used.
mutable provenance: BBa_I13507 for RFP and BBa_I6007 for TetR
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_B0012
component: BBa_K191007
component: BBa_C0040
component: BBa_I13507
component: BBa_R0040
component: BBa_B0034
component: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_B0034
sequence annotation: BBa_R0040
sequence annotation: BBa_I13507
sequence annotation: BBa_C0040
sequence annotation: BBa_B0010
sequence annotation: BBa_K191007
sequence: https://synbiohub.org/public/igem/BBa_K191005_sequence/1

BBa_B0012
type: Component
persistent identity: component2287513
display id: component2287513
version: 1
top level: BBa_K191005
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_K191007
type: Component
persistent identity: component2287504
display id: component2287504
version: 1
top level: BBa_K191005
access: public
definition: https://synbiohub.org/public/igem/BBa_K191007/1

BBa_C0040
type: Component
persistent identity: component2287510
display id: component2287510
version: 1
top level: BBa_K191005
access: public
definition: https://synbiohub.org/public/igem/BBa_C0040/1

BBa_I13507
type: Component
persistent identity: component2287533
display id: component2287533
version: 1
top level: BBa_K191005
access: public
definition: https://synbiohub.org/public/igem/BBa_I13507/1

BBa_R0040
type: Component
persistent identity: component2287517
display id: component2287517
version: 1
top level: BBa_K191005
access: public
definition: https://synbiohub.org/public/igem/BBa_R0040/1

BBa_B0034
type: Component
persistent identity: component2287506
display id: component2287506
version: 1
top level: BBa_K191005
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0010
type: Component
persistent identity: component2287511
display id: component2287511
version: 1
top level: BBa_K191005
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2287513
display id: annotation2287513
version: 1
top level: BBa_K191005
location: https://synbiohub.org/public/igem/BBa_K191005/annotation2287513/range2287513/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K191005/annotation2287513/range2287513/1
type: Range
persistent identity: range2287513
display id: range2287513
version: 1
direction: direction/forward
top level: BBa_K191005
start: 893
end: 933
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2287506
display id: annotation2287506
version: 1
top level: BBa_K191005
location: https://synbiohub.org/public/igem/BBa_K191005/annotation2287506/range2287506/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K191005/annotation2287506/range2287506/1
type: Range
persistent identity: range2287506
display id: range2287506
version: 1
direction: direction/reverse
top level: BBa_K191005
start: 94
end: 105
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2287517
display id: annotation2287517
version: 1
top level: BBa_K191005
location: https://synbiohub.org/public/igem/BBa_K191005/annotation2287517/range2287517/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_K191005/annotation2287517/range2287517/1
type: Range
persistent identity: range2287517
display id: range2287517
version: 1
direction: direction/forward
top level: BBa_K191005
start: 942
end: 995
orientation: inline

BBa_I13507
type: SequenceAnnotation
persistent identity: annotation2287533
display id: annotation2287533
version: 1
top level: BBa_K191005
location: https://synbiohub.org/public/igem/BBa_K191005/annotation2287533/range2287533/1
component: BBa_I13507

https://synbiohub.org/public/igem/BBa_K191005/annotation2287533/range2287533/1
type: Range
persistent identity: range2287533
display id: range2287533
version: 1
direction: direction/reverse
top level: BBa_K191005
start: 1004
end: 1864
orientation: inline

BBa_C0040
type: SequenceAnnotation
persistent identity: annotation2287510
display id: annotation2287510
version: 1
top level: BBa_K191005
location: https://synbiohub.org/public/igem/BBa_K191005/annotation2287510/range2287510/1
component: BBa_C0040

https://synbiohub.org/public/igem/BBa_K191005/annotation2287510/range2287510/1
type: Range
persistent identity: range2287510
display id: range2287510
version: 1
direction: direction/reverse
top level: BBa_K191005
start: 112
end: 796
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2287511
display id: annotation2287511
version: 1
top level: BBa_K191005
location: https://synbiohub.org/public/igem/BBa_K191005/annotation2287511/range2287511/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K191005/annotation2287511/range2287511/1
type: Range
persistent identity: range2287511
display id: range2287511
version: 1
direction: direction/forward
top level: BBa_K191005
start: 805
end: 884
orientation: inline

BBa_K191007
type: SequenceAnnotation
persistent identity: annotation2287504
display id: annotation2287504
version: 1
top level: BBa_K191005
location: https://synbiohub.org/public/igem/BBa_K191005/annotation2287504/range2287504/1
component: BBa_K191007

https://synbiohub.org/public/igem/BBa_K191005/annotation2287504/range2287504/1
type: Range
persistent identity: range2287504
display id: range2287504
version: 1
direction: direction/reverse
top level: BBa_K191005
start: 1
end: 85
orientation: inline

Tm-Fluo-li
type: ComponentDefinition
persistent identity: BBa K157014
display id: BBa_K157014
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Tm-Fluo-linker-cYFP
creator: iGEM Team Freiburg 2008
created: 2008-10-25T11:00:00Z
modified: 2015-05-08T01:10:54Z
discontinued: false
dominant: false
experience: experience/None
group u list: _232_
m user id: 0
owner id: 1673
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: C-terminal part of the Split fluorophore YFP fusioned to the transmembrane region of the Erbb1/EGF-receptor with the fluolinker in between.
mutable notes: none
mutable provenance: Derived by cloning of the parts BBa_K157002, BBa_K157009 and BBa_K157007. For further information see the description of the parts mentioned above.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: NgoMI
sequence annotation: AgeI
sequence annotation: Scar (Thr, Gly)
sequence annotation: Fluolinker
sequence annotation: Tm
sequence annotation: C-Term_YFP
sequence annotation: Tm-Fluolinker-cYFP
sequence: https://synbiohub.org/public/igem/BBa_K157014_sequence/1

NgoMI
type: SequenceAnnotation
persistent identity: annotation1989804
display id: annotation1989804
version: 1
top level: Tm-Fluo-li
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157014/annotation1989804/range1989804/1

https://synbiohub.org/public/igem/BBa_K157014/annotation1989804/range1989804/1
type: Range
persistent identity: range1989804
display id: range1989804
version: 1
direction: direction/forward
top level: Tm-Fluo-li
start: 4
end: 9
orientation: inline

AgeI
type: SequenceAnnotation
persistent identity: annotation1989805
display id: annotation1989805
version: 1
top level: Tm-Fluo-li
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157014/annotation1989805/range1989805/1

https://synbiohub.org/public/igem/BBa_K157014/annotation1989805/range1989805/1
type: Range
persistent identity: range1989805
display id: range1989805
version: 1
direction: direction/forward
top level: Tm-Fluo-li
start: 385
end: 390
orientation: inline

Scar (Thr, Gly)
type: SequenceAnnotation
persistent identity: annotation1990890
display id: annotation1990890
version: 1
top level: Tm-Fluo-li
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157014/annotation1990890/range1990890/1

https://synbiohub.org/public/igem/BBa_K157014/annotation1990890/range1990890/1
type: Range
persistent identity: range1990890
display id: range1990890
version: 1
direction: direction/forward
top level: Tm-Fluo-li
start: 79
end: 84
orientation: inline

Fluolinker
type: SequenceAnnotation
persistent identity: annotation1990824
display id: annotation1990824
version: 1
top level: Tm-Fluo-li
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157014/annotation1990824/range1990824/1

https://synbiohub.org/public/igem/BBa_K157014/annotation1990824/range1990824/1
type: Range
persistent identity: range1990824
display id: range1990824
version: 1
direction: direction/forward
top level: Tm-Fluo-li
start: 85
end: 135
orientation: inline

Tm
type: SequenceAnnotation
persistent identity: annotation1990821
display id: annotation1990821
version: 1
top level: Tm-Fluo-li
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157014/annotation1990821/range1990821/1

https://synbiohub.org/public/igem/BBa_K157014/annotation1990821/range1990821/1
type: Range
persistent identity: range1990821
display id: range1990821
version: 1
direction: direction/forward
top level: Tm-Fluo-li
start: 10
end: 78
orientation: inline

Scar (Thr, Gly)
type: SequenceAnnotation
persistent identity: annotation1990942
display id: annotation1990942
version: 1
top level: Tm-Fluo-li
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157014/annotation1990942/range1990942/1

https://synbiohub.org/public/igem/BBa_K157014/annotation1990942/range1990942/1
type: Range
persistent identity: range1990942
display id: range1990942
version: 1
direction: direction/forward
top level: Tm-Fluo-li
start: 136
end: 141
orientation: inline

C-Term_YFP
type: SequenceAnnotation
persistent identity: annotation1990876
display id: annotation1990876
version: 1
top level: Tm-Fluo-li
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157014/annotation1990876/range1990876/1

https://synbiohub.org/public/igem/BBa_K157014/annotation1990876/range1990876/1
type: Range
persistent identity: range1990876
display id: range1990876
version: 1
direction: direction/forward
top level: Tm-Fluo-li
start: 142
end: 384
orientation: inline

Tm-Fluolinker-cYFP
type: SequenceAnnotation
persistent identity: annotation1989803
display id: annotation1989803
version: 1
top level: Tm-Fluo-li
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K157014/annotation1989803/range1989803/1

https://synbiohub.org/public/igem/BBa_K157014/annotation1989803/range1989803/1
type: Range
persistent identity: range1989803
display id: range1989803
version: 1
direction: direction/forward
top level: Tm-Fluo-li
start: 1
end: 390
orientation: inline

SP-Lipocal
type: ComponentDefinition
persistent identity: BBa K157035
display id: BBa_K157035
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: SP-Lipocalin-GGGSlinker-Tm-nYFP
creator: iGEM Team Freiburg 2008
created: 2008-10-25T11:00:00Z
modified: 2015-05-08T01:10:55Z
discontinued: false
dominant: false
experience: experience/None
group u list: _232_
m user id: 0
owner id: 1673
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: A composite part consisting of the signal peptide of Erbb1/EGF-receptor, which ensures the transport to the cell membrane scFV-antiNIP, GGGSflexlinker (extracellular parts), the trans-membrane region of the Erbb1/EGF-receptor and the N-terminal part of the split fluorophor YFP(intracellular part).
mutable notes: none
mutable provenance: Derived by cloning of the parts BBa_K157001, BBa_K157004, BBa_K157013, BBa_K157002, BBa_K157008. For further information see the description of the parts mentioned above.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: Tm
sequence annotation: SP
sequence annotation: Scar (Thr, Gly)
sequence annotation: AgeI
sequence annotation: Lipocalin
sequence annotation: SP-Lipocalin-GGGSlinker-Tm-nYFP
sequence annotation: BBa_K157035
sequence annotation: GGGS-Linker
sequence annotation: NgoMI
sequence annotation: N-Term_YFP
sequence: https://synbiohub.org/public/igem/BBa_K157035_sequence/1

Tm
type: SequenceAnnotation
persistent identity: annotation1993378
display id: annotation1993378
version: 1
top level: SP-Lipocal
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157035/annotation1993378/range1993378/1

https://synbiohub.org/public/igem/BBa_K157035/annotation1993378/range1993378/1
type: Range
persistent identity: range1993378
display id: range1993378
version: 1
direction: direction/forward
top level: SP-Lipocal
start: 667
end: 735
orientation: inline

SP
type: SequenceAnnotation
persistent identity: annotation1993372
display id: annotation1993372
version: 1
top level: SP-Lipocal
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157035/annotation1993372/range1993372/1

https://synbiohub.org/public/igem/BBa_K157035/annotation1993372/range1993372/1
type: Range
persistent identity: range1993372
display id: range1993372
version: 1
direction: direction/forward
top level: SP-Lipocal
start: 10
end: 81
orientation: inline

Scar (Thr, Gly)
type: SequenceAnnotation
persistent identity: annotation1993375
display id: annotation1993375
version: 1
top level: SP-Lipocal
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157035/annotation1993375/range1993375/1

https://synbiohub.org/public/igem/BBa_K157035/annotation1993375/range1993375/1
type: Range
persistent identity: range1993375
display id: range1993375
version: 1
direction: direction/forward
top level: SP-Lipocal
start: 610
end: 615
orientation: inline

AgeI
type: SequenceAnnotation
persistent identity: annotation1993381
display id: annotation1993381
version: 1
top level: SP-Lipocal
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157035/annotation1993381/range1993381/1

https://synbiohub.org/public/igem/BBa_K157035/annotation1993381/range1993381/1
type: Range
persistent identity: range1993381
display id: range1993381
version: 1
direction: direction/forward
top level: SP-Lipocal
start: 1210
end: 1215
orientation: inline

Lipocalin
type: SequenceAnnotation
persistent identity: annotation1993374
display id: annotation1993374
version: 1
top level: SP-Lipocal
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157035/annotation1993374/range1993374/1

https://synbiohub.org/public/igem/BBa_K157035/annotation1993374/range1993374/1
type: Range
persistent identity: range1993374
display id: range1993374
version: 1
direction: direction/forward
top level: SP-Lipocal
start: 88
end: 609
orientation: inline

Scar (Thr, Gly)
type: SequenceAnnotation
persistent identity: annotation1993377
display id: annotation1993377
version: 1
top level: SP-Lipocal
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157035/annotation1993377/range1993377/1

https://synbiohub.org/public/igem/BBa_K157035/annotation1993377/range1993377/1
type: Range
persistent identity: range1993377
display id: range1993377
version: 1
direction: direction/forward
top level: SP-Lipocal
start: 661
end: 666
orientation: inline

Scar (Thr, Gly)
type: SequenceAnnotation
persistent identity: annotation1993379
display id: annotation1993379
version: 1
top level: SP-Lipocal
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157035/annotation1993379/range1993379/1

https://synbiohub.org/public/igem/BBa_K157035/annotation1993379/range1993379/1
type: Range
persistent identity: range1993379
display id: range1993379
version: 1
direction: direction/forward
top level: SP-Lipocal
start: 736
end: 741
orientation: inline

Scar (Thr, Gly)
type: SequenceAnnotation
persistent identity: annotation1993373
display id: annotation1993373
version: 1
top level: SP-Lipocal
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157035/annotation1993373/range1993373/1

https://synbiohub.org/public/igem/BBa_K157035/annotation1993373/range1993373/1
type: Range
persistent identity: range1993373
display id: range1993373
version: 1
direction: direction/forward
top level: SP-Lipocal
start: 82
end: 87
orientation: inline

SP-Lipocalin-GGGSlinker-Tm-nYFP
type: SequenceAnnotation
persistent identity: annotation1993370
display id: annotation1993370
version: 1
top level: SP-Lipocal
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K157035/annotation1993370/range1993370/1

https://synbiohub.org/public/igem/BBa_K157035/annotation1993370/range1993370/1
type: Range
persistent identity: range1993370
display id: range1993370
version: 1
direction: direction/forward
top level: SP-Lipocal
start: 1
end: 1215
orientation: inline

BBa_K157035
type: SequenceAnnotation
persistent identity: annotation1993382
display id: annotation1993382
version: 1
top level: SP-Lipocal
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K157035/annotation1993382/range1993382/1

https://synbiohub.org/public/igem/BBa_K157035/annotation1993382/range1993382/1
type: Range
persistent identity: range1993382
display id: range1993382
version: 1
direction: direction/reverse
top level: SP-Lipocal
start: 1
end: 1218
orientation: inline

GGGS-Linker
type: SequenceAnnotation
persistent identity: annotation1993376
display id: annotation1993376
version: 1
top level: SP-Lipocal
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157035/annotation1993376/range1993376/1

https://synbiohub.org/public/igem/BBa_K157035/annotation1993376/range1993376/1
type: Range
persistent identity: range1993376
display id: range1993376
version: 1
direction: direction/forward
top level: SP-Lipocal
start: 616
end: 660
orientation: inline

NgoMI
type: SequenceAnnotation
persistent identity: annotation1993371
display id: annotation1993371
version: 1
top level: SP-Lipocal
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157035/annotation1993371/range1993371/1

https://synbiohub.org/public/igem/BBa_K157035/annotation1993371/range1993371/1
type: Range
persistent identity: range1993371
display id: range1993371
version: 1
direction: direction/forward
top level: SP-Lipocal
start: 4
end: 9
orientation: inline

N-Term_YFP
type: SequenceAnnotation
persistent identity: annotation1993380
display id: annotation1993380
version: 1
top level: SP-Lipocal
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157035/annotation1993380/range1993380/1

https://synbiohub.org/public/igem/BBa_K157035/annotation1993380/range1993380/1
type: Range
persistent identity: range1993380
display id: range1993380
version: 1
direction: direction/forward
top level: SP-Lipocal
start: 742
end: 1209
orientation: inline

BBa_K1732020
type: ComponentDefinition
persistent identity: BBa K1732020
display id: BBa_K1732020
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: GFP-HIS
creator: Donna Lee
created: 2015-09-15T11:00:00Z
modified: 2016-01-25T01:12:20Z
discontinued: false
dominant: false
group u list: _2154_
m user id: 4206
owner id: 27195
owning group id: 9
status: status/Planning
bookmark: false
mutable description: GFP with a 6X His tag for easier protein purification.
mutable notes: Codon optimized for E.coli.
mutable provenance: Unknown
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: GFPCO
sequence annotation: 6x-His
sequence: https://synbiohub.org/public/igem/BBa_K1732020_sequence/1

GFPCO
type: SequenceAnnotation
persistent identity: annotation2462824
display id: annotation2462824
version: 1
top level: BBa_K1732020
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1732020/annotation2462824/range2462824/1

https://synbiohub.org/public/igem/BBa_K1732020/annotation2462824/range2462824/1
type: Range
persistent identity: range2462824
display id: range2462824
version: 1
direction: direction/forward
top level: BBa_K1732020
start: 1
end: 713
orientation: inline

6x-His
type: SequenceAnnotation
persistent identity: annotation2462825
display id: annotation2462825
version: 1
top level: BBa_K1732020
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1732020/annotation2462825/range2462825/1

https://synbiohub.org/public/igem/BBa_K1732020/annotation2462825/range2462825/1
type: Range
persistent identity: range2462825
display id: range2462825
version: 1
direction: direction/forward
top level: BBa_K1732020
start: 714
end: 731
orientation: inline

BBa_K1031804
type: ComponentDefinition
persistent identity: BBa K1031804
display id: BBa_K1031804
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Pu-B0032-sfGFP-Terminator (XylR)
creator: He Shuaixin
created: 2013-09-08T11:00:00Z
modified: 2015-05-08T01:08:48Z
discontinued: false
dominant: false
group u list: _1338_
m user id: 0
owner id: 15843
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Pu-32-sfGFP-TT (XylR)
mutable notes: molecular cloning
mutable provenance: synthesis, lab made
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: B0032
sequence annotation: B0015
sequence annotation: Pu (XylR)
sequence annotation: sfGFP
sequence: https://synbiohub.org/public/igem/BBa_K1031804_sequence/1

B0032
type: SequenceAnnotation
persistent identity: annotation2362532
display id: annotation2362532
version: 1
top level: BBa_K1031804
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1031804/annotation2362532/range2362532/1

https://synbiohub.org/public/igem/BBa_K1031804/annotation2362532/range2362532/1
type: Range
persistent identity: range2362532
display id: range2362532
version: 1
direction: direction/forward
top level: BBa_K1031804
start: 187
end: 199
orientation: inline

B0015
type: SequenceAnnotation
persistent identity: annotation2362534
display id: annotation2362534
version: 1
top level: BBa_K1031804
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1031804/annotation2362534/range2362534/1

https://synbiohub.org/public/igem/BBa_K1031804/annotation2362534/range2362534/1
type: Range
persistent identity: range2362534
display id: range2362534
version: 1
direction: direction/forward
top level: BBa_K1031804
start: 935
end: 1063
orientation: inline

Pu (XylR)
type: SequenceAnnotation
persistent identity: annotation2362531
display id: annotation2362531
version: 1
top level: BBa_K1031804
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1031804/annotation2362531/range2362531/1

https://synbiohub.org/public/igem/BBa_K1031804/annotation2362531/range2362531/1
type: Range
persistent identity: range2362531
display id: range2362531
version: 1
direction: direction/forward
top level: BBa_K1031804
start: 1
end: 180
orientation: inline

sfGFP
type: SequenceAnnotation
persistent identity: annotation2362533
display id: annotation2362533
version: 1
top level: BBa_K1031804
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1031804/annotation2362533/range2362533/1

https://synbiohub.org/public/igem/BBa_K1031804/annotation2362533/range2362533/1
type: Range
persistent identity: range2362533
display id: range2362533
version: 1
direction: direction/forward
top level: BBa_K1031804
start: 206
end: 928
orientation: inline

BBa_K1583017
type: ComponentDefinition
persistent identity: BBa K1583017
display id: BBa_K1583017
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Blue Fluorescent Protein (mTagBFP) without stopcodon
creator: Max van 't Hof
created: 2015-09-13T11:00:00Z
modified: 2016-01-25T01:00:29Z
discontinued: false
dominant: false
group u list: _2000_
m user id: 4206
owner id: 24478
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Biobrick part BBa_K592100 of the 2011 iGEM Uppsala team, without stopcodon.
mutable notes: The original stop codon needed to be removed to be able to attach SpyCatcher and His-tag.
mutable provenance: Part was developed by iGEM11_Uppsala-Sweden. It was produced by synthesis of part BBa_K1583113
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: BFP
sequence: https://synbiohub.org/public/igem/BBa_K1583017_sequence/1

BFP
type: SequenceAnnotation
persistent identity: annotation2474939
display id: annotation2474939
version: 1
top level: BBa_K1583017
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1583017/annotation2474939/range2474939/1

https://synbiohub.org/public/igem/BBa_K1583017/annotation2474939/range2474939/1
type: Range
persistent identity: range2474939
display id: range2474939
version: 1
direction: direction/forward
top level: BBa_K1583017
start: 1
end: 699
orientation: inline

eGFP-PTS
type: ComponentDefinition
persistent identity: BBa K678017
display id: BBa_K678017
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: eGFP-PTS-module
creator: DTU-Denmark-2
created: 2011-09-12T11:00:00Z
modified: 2015-05-08T01:13:04Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _882_
m user id: 0
owner id: 10556
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: to be announced
mutable notes: This part was designed for the Plug'n'Play with DNA assembly standard (RFC 80), therefore this part contains tails at the 5' and 3' ends as shown on the figure below.
mutable provenance: The Danish Cancer society
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: eGFP
sequence annotation: Linker 2
sequence annotation: PTS
sequence annotation: Kozak
sequence: https://synbiohub.org/public/igem/BBa_K678017_sequence/1

eGFP
type: SequenceAnnotation
persistent identity: annotation2127650
display id: annotation2127650
version: 1
top level: eGFP-PTS
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K678017/annotation2127650/range2127650/1

https://synbiohub.org/public/igem/BBa_K678017/annotation2127650/range2127650/1
type: Range
persistent identity: range2127650
display id: range2127650
version: 1
direction: direction/forward
top level: eGFP-PTS
start: 16
end: 717
orientation: inline

Linker 2
type: SequenceAnnotation
persistent identity: annotation2127652
display id: annotation2127652
version: 1
top level: eGFP-PTS
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678017/annotation2127652/range2127652/1

https://synbiohub.org/public/igem/BBa_K678017/annotation2127652/range2127652/1
type: Range
persistent identity: range2127652
display id: range2127652
version: 1
direction: direction/forward
top level: eGFP-PTS
start: 1
end: 8
orientation: inline

PTS
type: SequenceAnnotation
persistent identity: annotation2127651
display id: annotation2127651
version: 1
top level: eGFP-PTS
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K678017/annotation2127651/range2127651/1

https://synbiohub.org/public/igem/BBa_K678017/annotation2127651/range2127651/1
type: Range
persistent identity: range2127651
display id: range2127651
version: 1
direction: direction/forward
top level: eGFP-PTS
start: 712
end: 744
orientation: inline

Kozak
type: SequenceAnnotation
persistent identity: annotation2127795
display id: annotation2127795
version: 1
top level: eGFP-PTS
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678017/annotation2127795/range2127795/1

https://synbiohub.org/public/igem/BBa_K678017/annotation2127795/range2127795/1
type: Range
persistent identity: range2127795
display id: range2127795
version: 1
direction: direction/forward
top level: eGFP-PTS
start: 9
end: 19
orientation: inline

BBa_K259006
type: ComponentDefinition
persistent identity: BBa K259006
display id: BBa_K259006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: GFP-Terminator
creator: Petros Mina
created: 2009-09-08T11:00:00Z
modified: 2015-05-08T01:11:42Z
discontinued: false
dominant: false
group u list: _357_
m user id: 0
owner id: 5318
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: ==Part Description== This is a composite part made from Green Fluorescent Protein (GFP) and a double terminator. Green Fluorescent protein (originally from part [http://partsregistry.org/Part:BBa_E0040 BBa_E0040]) that will glow green when excited by the correct wavelengths and thus can be used as a reporter gene. This part is directly fused to a terminator (originally from part [http://partsregistry.org/Part:BBa_B0014 BBa_B0014]) so it is ideal to be fused at the end of your construct. ==Table of Statistics== {| style="color:white; background-color:#0066CC;" cellpadding="20" cellspacing="0" border="2" !Chassis !Monomer/Multimer !Aminoacid Length/Weight !Localisation !Structure !Excitation wavelengths !Emmision wavelengths |- |Prokaryotic and Eukaryotic |Bipartite |274 aminoacids | |beta-barrel(for GFP) hairpins for Terminator |501nm |511nm |} ==References== #Valdivia pmid=8707053
mutable notes: No additional design considerations. The parts were fused together following RFC10 assembly.
mutable provenance: GFP originates from jellyfish Aequeora victoria. For more details visit the [http://www.expasy.ch/uniprot/P42212 SwissProt entry]. The double terminator was designed by Reshma Shetty. For more details visis the [http://partsregistry.org/Part:BBa_B0014 original part page].
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_E0040
component: BBa_B0012
component: BBa_B0011
sequence annotation: BBa_E0040
sequence annotation: BBa_B0012
sequence annotation: BBa_B0011
sequence: https://synbiohub.org/public/igem/BBa_K259006_sequence/1

BBa_E0040
type: Component
persistent identity: component2020529
display id: component2020529
version: 1
top level: BBa_K259006
access: public
definition: GFP

BBa_B0012
type: Component
persistent identity: component2020530
display id: component2020530
version: 1
top level: BBa_K259006
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0011
type: Component
persistent identity: component2020534
display id: component2020534
version: 1
top level: BBa_K259006
access: public
definition: https://synbiohub.org/public/igem/BBa_B0011/1

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation2020529
display id: annotation2020529
version: 1
top level: BBa_K259006
location: https://synbiohub.org/public/igem/BBa_K259006/annotation2020529/range2020529/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_K259006/annotation2020529/range2020529/1
type: Range
persistent identity: range2020529
display id: range2020529
version: 1
direction: direction/reverse
top level: BBa_K259006
start: 1
end: 720
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2020530
display id: annotation2020530
version: 1
top level: BBa_K259006
location: https://synbiohub.org/public/igem/BBa_K259006/annotation2020530/range2020530/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K259006/annotation2020530/range2020530/1
type: Range
persistent identity: range2020530
display id: range2020530
version: 1
direction: direction/forward
top level: BBa_K259006
start: 729
end: 769
orientation: inline

BBa_B0011
type: SequenceAnnotation
persistent identity: annotation2020534
display id: annotation2020534
version: 1
top level: BBa_K259006
location: https://synbiohub.org/public/igem/BBa_K259006/annotation2020534/range2020534/1
component: BBa_B0011

https://synbiohub.org/public/igem/BBa_K259006/annotation2020534/range2020534/1
type: Range
persistent identity: range2020534
display id: range2020534
version: 1
direction: direction/forward
top level: BBa_K259006
start: 778
end: 823
orientation: inline

BBa_K157007
type: ComponentDefinition
persistent identity: BBa K157007
display id: BBa_K157007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Split-Venus-cYFP
creator: iGEM Team Freiburg 2008
created: 2008-10-25T11:00:00Z
modified: 2015-05-08T01:10:54Z
discontinued: false
dominant: false
experience: experience/None
group u list: _232_
m user id: 0
owner id: 1673
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: C-terminal fragment (Amino acids157-237) of the monomeric[5] yellow fluorescent protein variant ???Venus??? [4], designed for fusion/BiFC[1,3]. Reassembly with Part Bba_K157008 (Split-Venus-nYFP) generates the complete, working YFP ???Venus???, whereas combination with the n-terminal fragment (Part Bba_K157006) of the monomeric cyan fluorescent Protein ???CeruleanR206K??? (Part Bba_I757009) results in green fluorescence[2]. - [1] Chang-Deng Hu, Yurii Chinenov, Tom K. Kerppola: ???Visualization of Interactions among bZIP and Rel Family Proteins in Living Cells Using Bimolecular Fluorescence Complementation???, Molecular Cell, Vol. 9, 789???798, April, 2002 - [2] Chang Deng Hu, Tom K. Kerppola: ???Simultaneous visualization of multiple protein interactions in living cells using multicolor fluorescence complementation analysis???, Nat Biotechnol. 2003 May; 21(5):539-545 (doi:10. 1038/nbt816) -[3] Tom K. Kerppola: ???Design and implementation of bimolecular fluorescence complementation (BiFC) assays for the visualization of protein interactions in living cells???, Nat Protoc. 2006;1(3):1278-1286 (doi:10.1038/nprot.2006.201) -[4]Nagai, T. et al. ???A variant of yellow fluorescent protein with fast and efficient maturation for cell-biological applications??? J. Biol. Chem. 276, 29188-29194, 2001 -[5]Roger Y. Tsien et al. ???Creating new fluorescent probes for cell biology???, Nature Biotechnology Reviews, Vol. 3, 906-918, 2002
mutable notes: Between BioBrick 1.0 sites this part is flanked 5' by NgoMI(=NgoMIV) and 3' AgeI(=PinAI) to facilitate in frame cloning for protein fusions.
mutable provenance: Gene synthesis by GeneArt, optimized for expression in homo sapiens.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: C-terminal portion of split venus YFP
sequence: https://synbiohub.org/public/igem/BBa_K157007_sequence/1

C-terminal portion of split venus YFP
type: SequenceAnnotation
persistent identity: annotation2064735
display id: annotation2064735
version: 1
top level: BBa_K157007
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157007/annotation2064735/range2064735/1

https://synbiohub.org/public/igem/BBa_K157007/annotation2064735/range2064735/1
type: Range
persistent identity: range2064735
display id: range2064735
version: 1
direction: direction/forward
top level: BBa_K157007
start: 1
end: 243
orientation: inline

BBa_K180015
type: ComponentDefinition
persistent identity: BBa K180015
display id: BBa_K180015
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Paper - Primary plasmid (part 2) [tac pR, GFP and RhlI generator]
creator: Richard Wilson, Stephanie Chambers
created: 2009-10-18T11:00:00Z
modified: 2015-05-08T01:11:06Z
discontinued: false
dominant: false
experience: experience/None
group u list: _278_
m user id: 0
owner id: 4082
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Part of the iGEM2009 Team:Southampton rock-paper-scissors 'rock' primary plasmid.
mutable notes: Regulated expression of RhlI (C0170) and GFP (E0040) by tac pR (K180000), controlled by presence of lac/IPTG or trp in the cell.
mutable provenance: See relevant basic biobrick parts (K180000, B0034, E0040, B0015).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0012
component: BBa_B0034
component: BBa_B0010
component: BBa_C0170
component: BBa_E0040
component: BBa_K180000
sequence annotation: BBa_B0012
sequence annotation: BBa_E0040
sequence annotation: BBa_B0034
sequence annotation: BBa_B0010
sequence annotation: BBa_C0170
sequence annotation: BBa_K180000
sequence: https://synbiohub.org/public/igem/BBa_K180015_sequence/1

BBa_B0012
type: Component
persistent identity: component2053969
display id: component2053969
version: 1
top level: BBa_K180015
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0034
type: Component
persistent identity: component2053964
display id: component2053964
version: 1
top level: BBa_K180015
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0034
type: Component
persistent identity: component2053960
display id: component2053960
version: 1
top level: BBa_K180015
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0010
type: Component
persistent identity: component2053967
display id: component2053967
version: 1
top level: BBa_K180015
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_C0170
type: Component
persistent identity: component2053966
display id: component2053966
version: 1
top level: BBa_K180015
access: public
definition: https://synbiohub.org/public/igem/BBa_C0170/1

BBa_E0040
type: Component
persistent identity: component2053962
display id: component2053962
version: 1
top level: BBa_K180015
access: public
definition: GFP

BBa_K180000
type: Component
persistent identity: component2053958
display id: component2053958
version: 1
top level: BBa_K180015
access: public
definition: https://synbiohub.org/public/igem/BBa_K180000/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2053969
display id: annotation2053969
version: 1
top level: BBa_K180015
location: https://synbiohub.org/public/igem/BBa_K180015/annotation2053969/range2053969/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K180015/annotation2053969/range2053969/1
type: Range
persistent identity: range2053969
display id: range2053969
version: 1
direction: direction/forward
top level: BBa_K180015
start: 2925
end: 2965
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation2053962
display id: annotation2053962
version: 1
top level: BBa_K180015
location: https://synbiohub.org/public/igem/BBa_K180015/annotation2053962/range2053962/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_K180015/annotation2053962/range2053962/1
type: Range
persistent identity: range2053962
display id: range2053962
version: 1
direction: direction/reverse
top level: BBa_K180015
start: 1474
end: 2193
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2053960
display id: annotation2053960
version: 1
top level: BBa_K180015
location: https://synbiohub.org/public/igem/BBa_K180015/annotation2053960/range2053960/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K180015/annotation2053960/range2053960/1
type: Range
persistent identity: range2053960
display id: range2053960
version: 1
direction: direction/reverse
top level: BBa_K180015
start: 1456
end: 1467
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2053967
display id: annotation2053967
version: 1
top level: BBa_K180015
location: https://synbiohub.org/public/igem/BBa_K180015/annotation2053967/range2053967/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K180015/annotation2053967/range2053967/1
type: Range
persistent identity: range2053967
display id: range2053967
version: 1
direction: direction/forward
top level: BBa_K180015
start: 2837
end: 2916
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2053964
display id: annotation2053964
version: 1
top level: BBa_K180015
location: https://synbiohub.org/public/igem/BBa_K180015/annotation2053964/range2053964/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K180015/annotation2053964/range2053964/1
type: Range
persistent identity: range2053964
display id: range2053964
version: 1
direction: direction/reverse
top level: BBa_K180015
start: 2202
end: 2213
orientation: inline

BBa_C0170
type: SequenceAnnotation
persistent identity: annotation2053966
display id: annotation2053966
version: 1
top level: BBa_K180015
location: https://synbiohub.org/public/igem/BBa_K180015/annotation2053966/range2053966/1
component: BBa_C0170

https://synbiohub.org/public/igem/BBa_K180015/annotation2053966/range2053966/1
type: Range
persistent identity: range2053966
display id: range2053966
version: 1
direction: direction/reverse
top level: BBa_K180015
start: 2220
end: 2828
orientation: inline

BBa_K180000
type: SequenceAnnotation
persistent identity: annotation2053958
display id: annotation2053958
version: 1
top level: BBa_K180015
location: https://synbiohub.org/public/igem/BBa_K180015/annotation2053958/range2053958/1
component: BBa_K180000

https://synbiohub.org/public/igem/BBa_K180015/annotation2053958/range2053958/1
type: Range
persistent identity: range2053958
display id: range2053958
version: 1
direction: direction/reverse
top level: BBa_K180015
start: 1
end: 1447
orientation: inline

BBa_K1159301
type: ComponentDefinition
persistent identity: BBa K1159301
display id: BBa_K1159301
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Super Yellow Fluorescent Protein 2 (SYFP2) in RFC[25]
creator: Dong-Jiunn Jeffery TRUONG
created: 2013-09-13T11:00:00Z
modified: 2016-01-26T02:17:14Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1471_
m user id: 4206
owner id: 11507
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: Improved part of BBa_K864100 which originally cannot be fused to other protein domains. This improved version is flanked by RFC[25] pre- and suffix for further protein fusions.
mutable notes: x
mutable provenance: ''Aequorea victoria''
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: SYFP2
sequence: https://synbiohub.org/public/igem/BBa_K1159301_sequence/1

SYFP2
type: SequenceAnnotation
persistent identity: annotation2343586
display id: annotation2343586
version: 1
top level: BBa_K1159301
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1159301/annotation2343586/range2343586/1

https://synbiohub.org/public/igem/BBa_K1159301/annotation2343586/range2343586/1
type: Range
persistent identity: range2343586
display id: range2343586
version: 1
direction: direction/forward
top level: BBa_K1159301
start: 1
end: 714
orientation: inline

BBa_K1321366
type: ComponentDefinition
persistent identity: BBa K1321366
display id: BBa_K1321366
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CBDcex fused to sfGFP with T7 promoter
creator: Gabriella Santosa
created: 2014-10-08T11:00:00Z
modified: 2016-01-26T09:46:05Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1696_
m user id: 4206
owner id: 20961
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: GATC
mutable notes: GATC
mutable provenance: GATC
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1321338
component: BBa_K1319106
component: BBa_K1321349
sequence annotation: BBa_K1319106
sequence annotation: BBa_K1321349
sequence annotation: BBa_K1321338
sequence: https://synbiohub.org/public/igem/BBa_K1321366_sequence/1

BBa_K1321338
type: Component
persistent identity: component2415724
display id: component2415724
version: 1
top level: BBa_K1321366
access: public
definition: https://synbiohub.org/public/igem/BBa_K1321338/1

BBa_K1319106
type: Component
persistent identity: component2415726
display id: component2415726
version: 1
top level: BBa_K1321366
access: public
definition: https://synbiohub.org/public/igem/BBa_K1319106/1

BBa_K1321349
type: Component
persistent identity: component2415732
display id: component2415732
version: 1
top level: BBa_K1321366
access: public
definition: https://synbiohub.org/public/igem/BBa_K1321349/1

BBa_K1319106
type: SequenceAnnotation
persistent identity: annotation2415726
display id: annotation2415726
version: 1
top level: BBa_K1321366
location: https://synbiohub.org/public/igem/BBa_K1321366/annotation2415726/range2415726/1
component: BBa_K1319106

https://synbiohub.org/public/igem/BBa_K1321366/annotation2415726/range2415726/1
type: Range
persistent identity: range2415726
display id: range2415726
version: 1
direction: direction/reverse
top level: BBa_K1321366
start: 44
end: 58
orientation: inline

BBa_K1321349
type: SequenceAnnotation
persistent identity: annotation2415732
display id: annotation2415732
version: 1
top level: BBa_K1321366
location: https://synbiohub.org/public/igem/BBa_K1321366/annotation2415732/range2415732/1
component: BBa_K1321349

https://synbiohub.org/public/igem/BBa_K1321366/annotation2415732/range2415732/1
type: Range
persistent identity: range2415732
display id: range2415732
version: 1
direction: direction/reverse
top level: BBa_K1321366
start: 59
end: 1102
orientation: inline

BBa_K1321338
type: SequenceAnnotation
persistent identity: annotation2415724
display id: annotation2415724
version: 1
top level: BBa_K1321366
location: https://synbiohub.org/public/igem/BBa_K1321366/annotation2415724/range2415724/1
component: BBa_K1321338

https://synbiohub.org/public/igem/BBa_K1321366/annotation2415724/range2415724/1
type: Range
persistent identity: range2415724
display id: range2415724
version: 1
direction: direction/reverse
top level: BBa_K1321366
start: 1
end: 43
orientation: inline

BBa_K1399015
type: ComponentDefinition
persistent identity: BBa K1399015
display id: BBa_K1399015
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Plac-GFP(LVA)
creator: Anna Stikane
created: 2014-09-18T11:00:00Z
modified: 2015-05-08T01:10:16Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1777_
m user id: 0
owner id: 22477
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: Lactose/IPTG inducible promoter with GFP reporter tagged with AAV-ssrA degradation tag followed by terminator. The tagged GFP is actively degraded within cell, thus provides better temporal resolution of green fluorescence and promoter activity.
mutable notes: Part was assembled using BrickClip assembly (BBF RFC104) using other biobrick parts as templates. BrickClip assembly is a special case of more general Paperclip assembly method.[1]
mutable provenance: other biobricks
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_K1399004
component: BBa_R0010
component: BBa_B0015
component: BBa_B0034
sequence annotation: BBa_R0010
sequence annotation: BBa_K1399004
sequence annotation: BBa_B0034
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_K1399015_sequence/1

BBa_K1399004
type: Component
persistent identity: component2384078
display id: component2384078
version: 1
top level: BBa_K1399015
access: public
definition: BBa_K1399004

BBa_R0010
type: Component
persistent identity: component2384064
display id: component2384064
version: 1
top level: BBa_K1399015
access: public
definition: https://synbiohub.org/public/igem/BBa_R0010/1

BBa_B0015
type: Component
persistent identity: component2384085
display id: component2384085
version: 1
top level: BBa_K1399015
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_B0034
type: Component
persistent identity: component2384072
display id: component2384072
version: 1
top level: BBa_K1399015
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation2384064
display id: annotation2384064
version: 1
top level: BBa_K1399015
location: https://synbiohub.org/public/igem/BBa_K1399015/annotation2384064/range2384064/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_K1399015/annotation2384064/range2384064/1
type: Range
persistent identity: range2384064
display id: range2384064
version: 1
direction: direction/forward
top level: BBa_K1399015
start: 1
end: 200
orientation: inline

BBa_K1399004
type: SequenceAnnotation
persistent identity: annotation2384078
display id: annotation2384078
version: 1
top level: BBa_K1399015
location: https://synbiohub.org/public/igem/BBa_K1399015/annotation2384078/range2384078/1
component: BBa_K1399004

https://synbiohub.org/public/igem/BBa_K1399015/annotation2384078/range2384078/1
type: Range
persistent identity: range2384078
display id: range2384078
version: 1
direction: direction/reverse
top level: BBa_K1399015
start: 227
end: 979
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2384072
display id: annotation2384072
version: 1
top level: BBa_K1399015
location: https://synbiohub.org/public/igem/BBa_K1399015/annotation2384072/range2384072/1
component: BBa_B0034

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type: Range
persistent identity: range2384072
display id: range2384072
version: 1
direction: direction/reverse
top level: BBa_K1399015
start: 209
end: 220
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2384085
display id: annotation2384085
version: 1
top level: BBa_K1399015
location: https://synbiohub.org/public/igem/BBa_K1399015/annotation2384085/range2384085/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K1399015/annotation2384085/range2384085/1
type: Range
persistent identity: range2384085
display id: range2384085
version: 1
direction: direction/reverse
top level: BBa_K1399015
start: 988
end: 1116
orientation: inline

BBa_K180012
type: ComponentDefinition
persistent identity: BBa K180012
display id: BBa_K180012
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Rock - Primary plasmid (part 2) [tac pR, mCherry and LasI generator]
creator: Richard Wilson, Stephanie Chambers
created: 2009-10-18T11:00:00Z
modified: 2015-05-08T01:11:06Z
discontinued: false
dominant: false
experience: experience/None
group u list: _278_
m user id: 0
owner id: 4082
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Part of the iGEM2009 Team:Southampton rock-paper-scissors 'rock' primary plasmid.
mutable notes: Regulated expression of LasI (C0178) and mCherry (K180008) by tac pR (K180000), controlled by presence of lac/IPTG or trp in the cell.
mutable provenance: See relevant basic biobrick parts (K180000, B0034, K180008, B0015)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K180000
component: BBa_C0178
component: BBa_B0034
component: BBa_B0012
component: BBa_B0010
component: BBa_K180008
sequence annotation: BBa_K180008
sequence annotation: BBa_B0010
sequence annotation: BBa_B0034
sequence annotation: BBa_K180000
sequence annotation: BBa_C0178
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_K180012_sequence/1

BBa_K180000
type: Component
persistent identity: component2053924
display id: component2053924
version: 1
top level: BBa_K180012
access: public
definition: https://synbiohub.org/public/igem/BBa_K180000/1

BBa_C0178
type: Component
persistent identity: component2053932
display id: component2053932
version: 1
top level: BBa_K180012
access: public
definition: https://synbiohub.org/public/igem/BBa_C0178/1

BBa_B0034
type: Component
persistent identity: component2053930
display id: component2053930
version: 1
top level: BBa_K180012
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0034
type: Component
persistent identity: component2053926
display id: component2053926
version: 1
top level: BBa_K180012
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0012
type: Component
persistent identity: component2053935
display id: component2053935
version: 1
top level: BBa_K180012
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0010
type: Component
persistent identity: component2053933
display id: component2053933
version: 1
top level: BBa_K180012
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_K180008
type: Component
persistent identity: component2053928
display id: component2053928
version: 1
top level: BBa_K180012
access: public
definition: BBa_K180008

BBa_K180008
type: SequenceAnnotation
persistent identity: annotation2053928
display id: annotation2053928
version: 1
top level: BBa_K180012
location: https://synbiohub.org/public/igem/BBa_K180012/annotation2053928/range2053928/1
component: BBa_K180008

https://synbiohub.org/public/igem/BBa_K180012/annotation2053928/range2053928/1
type: Range
persistent identity: range2053928
display id: range2053928
version: 1
direction: direction/reverse
top level: BBa_K180012
start: 1474
end: 2181
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2053933
display id: annotation2053933
version: 1
top level: BBa_K180012
location: https://synbiohub.org/public/igem/BBa_K180012/annotation2053933/range2053933/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K180012/annotation2053933/range2053933/1
type: Range
persistent identity: range2053933
display id: range2053933
version: 1
direction: direction/forward
top level: BBa_K180012
start: 2825
end: 2904
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2053926
display id: annotation2053926
version: 1
top level: BBa_K180012
location: https://synbiohub.org/public/igem/BBa_K180012/annotation2053926/range2053926/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K180012/annotation2053926/range2053926/1
type: Range
persistent identity: range2053926
display id: range2053926
version: 1
direction: direction/reverse
top level: BBa_K180012
start: 1456
end: 1467
orientation: inline

BBa_K180000
type: SequenceAnnotation
persistent identity: annotation2053924
display id: annotation2053924
version: 1
top level: BBa_K180012
location: https://synbiohub.org/public/igem/BBa_K180012/annotation2053924/range2053924/1
component: BBa_K180000

https://synbiohub.org/public/igem/BBa_K180012/annotation2053924/range2053924/1
type: Range
persistent identity: range2053924
display id: range2053924
version: 1
direction: direction/reverse
top level: BBa_K180012
start: 1
end: 1447
orientation: inline

BBa_C0178
type: SequenceAnnotation
persistent identity: annotation2053932
display id: annotation2053932
version: 1
top level: BBa_K180012
location: https://synbiohub.org/public/igem/BBa_K180012/annotation2053932/range2053932/1
component: BBa_C0178

https://synbiohub.org/public/igem/BBa_K180012/annotation2053932/range2053932/1
type: Range
persistent identity: range2053932
display id: range2053932
version: 1
direction: direction/reverse
top level: BBa_K180012
start: 2208
end: 2816
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2053930
display id: annotation2053930
version: 1
top level: BBa_K180012
location: https://synbiohub.org/public/igem/BBa_K180012/annotation2053930/range2053930/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K180012/annotation2053930/range2053930/1
type: Range
persistent identity: range2053930
display id: range2053930
version: 1
direction: direction/reverse
top level: BBa_K180012
start: 2190
end: 2201
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2053935
display id: annotation2053935
version: 1
top level: BBa_K180012
location: https://synbiohub.org/public/igem/BBa_K180012/annotation2053935/range2053935/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K180012/annotation2053935/range2053935/1
type: Range
persistent identity: range2053935
display id: range2053935
version: 1
direction: direction/forward
top level: BBa_K180012
start: 2913
end: 2953
orientation: inline

BBa_K1819006
type: ComponentDefinition
persistent identity: BBa K1819006
display id: BBa_K1819006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Constitutive promoter (J23106) and GFP (S05308)
creator: La??s Ribovski
created: 2015-09-15T11:00:00Z
modified: 2015-09-16T05:21:36Z
discontinued: false
dominant: false
group u list: _2244_
m user id: 21190
owner id: 21190
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Constitutive promotor from Anderson library (BBa_J23106) assembled to green fluorescent protein attached to linker (BBa_S05308). This part was used to characterize BBa_K1819000 and compare its efficiency to green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (BBa_E0040).
mutable notes: No considerations
mutable provenance: This part results from the assembly of BBa_J23106 and BBa_S05308
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_J23106
component: BBa_S05308
sequence annotation: BBa_J23106
sequence annotation: BBa_S05308
sequence: https://synbiohub.org/public/igem/BBa_K1819006_sequence/1

BBa_J23106
type: Component
persistent identity: component2462462
display id: component2462462
version: 1
top level: BBa_K1819006
access: public
definition: https://synbiohub.org/public/igem/BBa_J23106/1

BBa_S05308
type: Component
persistent identity: component2462473
display id: component2462473
version: 1
top level: BBa_K1819006
access: public
definition: https://synbiohub.org/public/igem/BBa_S05308/1

BBa_J23106
type: SequenceAnnotation
persistent identity: annotation2462462
display id: annotation2462462
version: 1
top level: BBa_K1819006
location: https://synbiohub.org/public/igem/BBa_K1819006/annotation2462462/range2462462/1
component: BBa_J23106

https://synbiohub.org/public/igem/BBa_K1819006/annotation2462462/range2462462/1
type: Range
persistent identity: range2462462
display id: range2462462
version: 1
direction: direction/reverse
top level: BBa_K1819006
start: 1
end: 35
orientation: inline

BBa_S05308
type: SequenceAnnotation
persistent identity: annotation2462473
display id: annotation2462473
version: 1
top level: BBa_K1819006
location: https://synbiohub.org/public/igem/BBa_K1819006/annotation2462473/range2462473/1
component: BBa_S05308

https://synbiohub.org/public/igem/BBa_K1819006/annotation2462473/range2462473/1
type: Range
persistent identity: range2462473
display id: range2462473
version: 1
direction: direction/reverse
top level: BBa_K1819006
start: 44
end: 930
orientation: inline

BBa_K1399007
type: ComponentDefinition
persistent identity: BBa K1399007
display id: BBa_K1399007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: GFP (mut3b) with SsrA-DAS+2 degradation tag
creator: Anna Stikane
created: 2014-09-18T11:00:00Z
modified: 2015-05-08T01:10:16Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1777_
m user id: 0
owner id: 22477
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: GFP (mut3b) (see part BBa_E0040) with added engineered NYADAS-ssrA degradation tag (see part BBa_M0050). The tag increases GFP turn-over rate, thus providing better temporal resolution of green fluorescence. In the same time, maximal fluorescence amplitudes will be lower as newly formed protein is degraded as soon as it is formed. SsrA tags encode peptide sequence that and is recognized by ClpA and ClpX unfoldases and ClpX mediator SspB.[1] ClpA and ClpX then form a proteosome-like complex with ClpP protease and the protein is degraded.[1] The final three residues of the tag determines the strength of interaction with ClpX and thus the final protein degradation rate.[2] The NYADAS tag encodes peptide sequence AANDENYNYDAS is reported to have low affinity to ClpX thus its mediated degradation very much depends on the concentration of SspB (ClpX mediator).[1] The two additional residues ???NY??? extends tag between SspB and ClpX binding site, thus preventing clash when both these protein are bound to tag.[3] However, be aware that exact protein degradation rate is influenced by multiple other factors: ClpXP and ClpAP protease concentrations, protein stability, Km of binding to the protease, temperature [4]. ===References=== [1] Flynn, J. M. et al. Overlapping recognition determinants within the ssrA degradation tag allow modulation of proteolysis. Proc. Natl. Acad. Sci. U. S. A. 98, 10584???9 (2001). [2] Andersen, J. B. et al. New unstable variants of green fluorescent protein for studies of transient gene expression in bacteria. Appl. Environ. Microbiol. 64, 2240???6 (1998). [3] McGinness, K. E., Baker, T. a & Sauer, R. T. Engineering controllable protein degradation. Mol. Cell 22, 701???7 (2006). [4] Purcell, O., Grierson, C. S., Bernardo, M. Di & Savery, N. J. Temperature dependence of ssrA-tag mediated protein degradation. J. Biol. Eng. 6, 10 (2012).
mutable notes: The tag was attached to GFP using PCR and MABEL (mutagenesis with blunt-end ligation), thus avoiding introduction of additional residues and restriction site. Different parts of the tag are recognized by different proteins, for example, the final 3 residues (DAS in this case) are recognized by ClpX, whereas first 4 residues of the tag are required for efficient SspB binding.[1] Thus modifications of these critical residues alter the efficacy with what different proteases bind to it.
mutable provenance: GFP comes from part BBa_E0040, the tag sequence was obtained from part BBa_M0051.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: stop
sequence annotation: start
sequence annotation: GFP (mut3b)
sequence annotation: NYADAS-ssrA
sequence: https://synbiohub.org/public/igem/BBa_K1399007_sequence/1

stop
type: SequenceAnnotation
persistent identity: annotation2383926
display id: annotation2383926
version: 1
top level: BBa_K1399007
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1399007/annotation2383926/range2383926/1

https://synbiohub.org/public/igem/BBa_K1399007/annotation2383926/range2383926/1
type: Range
persistent identity: range2383926
display id: range2383926
version: 1
direction: direction/forward
top level: BBa_K1399007
start: 754
end: 756
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2383923
display id: annotation2383923
version: 1
top level: BBa_K1399007
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1399007/annotation2383923/range2383923/1

https://synbiohub.org/public/igem/BBa_K1399007/annotation2383923/range2383923/1
type: Range
persistent identity: range2383923
display id: range2383923
version: 1
direction: direction/forward
top level: BBa_K1399007
start: 1
end: 3
orientation: inline

GFP (mut3b)
type: SequenceAnnotation
persistent identity: annotation2383924
display id: annotation2383924
version: 1
top level: BBa_K1399007
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1399007/annotation2383924/range2383924/1

https://synbiohub.org/public/igem/BBa_K1399007/annotation2383924/range2383924/1
type: Range
persistent identity: range2383924
display id: range2383924
version: 1
direction: direction/forward
top level: BBa_K1399007
start: 4
end: 714
orientation: inline

NYADAS-ssrA
type: SequenceAnnotation
persistent identity: annotation2383925
display id: annotation2383925
version: 1
top level: BBa_K1399007
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1399007/annotation2383925/range2383925/1

https://synbiohub.org/public/igem/BBa_K1399007/annotation2383925/range2383925/1
type: Range
persistent identity: range2383925
display id: range2383925
version: 1
direction: direction/forward
top level: BBa_K1399007
start: 715
end: 753
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2383927
display id: annotation2383927
version: 1
top level: BBa_K1399007
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1399007/annotation2383927/range2383927/1

https://synbiohub.org/public/igem/BBa_K1399007/annotation2383927/range2383927/1
type: Range
persistent identity: range2383927
display id: range2383927
version: 1
direction: direction/forward
top level: BBa_K1399007
start: 757
end: 759
orientation: inline

BBa_K106671
type: ComponentDefinition
persistent identity: BBa K106671
display id: BBa_K106671
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: GFP, Aar1 AD part
creator: Jacinto Chen
created: 2008-10-28T12:00:00Z
modified: 2016-01-26T09:50:57Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _226_
m user id: 4206
owner id: 3418
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This is the coding sequence of a yeast optimized GFP with BD ends for AarI cloning.
mutable notes: As an AD part, there is no ATG, and the stop codon is included. For more information on AarI cloning, please refer to the UCSF 2008 igem page.
mutable provenance: PCR amplified from lab plasmid stocks.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: BBa_K106671
sequence: https://synbiohub.org/public/igem/BBa_K106671_sequence/1

BBa_K106671
type: SequenceAnnotation
persistent identity: annotation1993686
display id: annotation1993686
version: 1
top level: BBa_K106671
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K106671/annotation1993686/range1993686/1

https://synbiohub.org/public/igem/BBa_K106671/annotation1993686/range1993686/1
type: Range
persistent identity: range1993686
display id: range1993686
version: 1
direction: direction/reverse
top level: BBa_K106671
start: 1
end: 714
orientation: inline

BBa_K216011
type: ComponentDefinition
persistent identity: BBa K216011
display id: BBa_K216011
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PompC+EYFP
creator: Edinburgh iGEM 2009
created: 2009-10-15T11:00:00Z
modified: 2015-05-08T01:11:30Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _317_
m user id: 0
owner id: 837
owning group id: 163
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This is the ompC promoter, controlled by the response regulator OmpR and sensor kinase EnvZ, joined to Enhanced Yellow Fluorescent Protein.
mutable notes: No special considerations.
mutable provenance: Made by joining ompC promoter BioBrick BBa_R0082 to a PCR product including RBS BBa_B0034 and EYFP BBa_E0030.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_E0030
component: BBa_R0082
component: BBa_B0034
sequence annotation: BBa_R0082
sequence annotation: BBa_B0034
sequence annotation: BBa_E0030
sequence: https://synbiohub.org/public/igem/BBa_K216011_sequence/1

BBa_E0030
type: Component
persistent identity: component2047043
display id: component2047043
version: 1
top level: BBa_K216011
access: public
definition: eyfp

BBa_R0082
type: Component
persistent identity: component2047040
display id: component2047040
version: 1
top level: BBa_K216011
access: public
definition: https://synbiohub.org/public/igem/BBa_R0082/1

BBa_B0034
type: Component
persistent identity: component2047042
display id: component2047042
version: 1
top level: BBa_K216011
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_R0082
type: SequenceAnnotation
persistent identity: annotation2047040
display id: annotation2047040
version: 1
top level: BBa_K216011
location: https://synbiohub.org/public/igem/BBa_K216011/annotation2047040/range2047040/1
component: BBa_R0082

https://synbiohub.org/public/igem/BBa_K216011/annotation2047040/range2047040/1
type: Range
persistent identity: range2047040
display id: range2047040
version: 1
direction: direction/reverse
top level: BBa_K216011
start: 1
end: 108
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2047042
display id: annotation2047042
version: 1
top level: BBa_K216011
location: https://synbiohub.org/public/igem/BBa_K216011/annotation2047042/range2047042/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K216011/annotation2047042/range2047042/1
type: Range
persistent identity: range2047042
display id: range2047042
version: 1
direction: direction/reverse
top level: BBa_K216011
start: 117
end: 128
orientation: inline

BBa_E0030
type: SequenceAnnotation
persistent identity: annotation2047043
display id: annotation2047043
version: 1
top level: BBa_K216011
location: https://synbiohub.org/public/igem/BBa_K216011/annotation2047043/range2047043/1
component: BBa_E0030

https://synbiohub.org/public/igem/BBa_K216011/annotation2047043/range2047043/1
type: Range
persistent identity: range2047043
display id: range2047043
version: 1
direction: direction/reverse
top level: BBa_K216011
start: 135
end: 857
orientation: inline

BBa_K1399004
type: ComponentDefinition
persistent identity: BBa K1399004
display id: BBa_K1399004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: GFP (mut3b) with LVA-ssrA degradation tag
creator: Anna Stikane
created: 2014-09-18T11:00:00Z
modified: 2015-06-01T07:07:29Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1777_
m user id: 4206
owner id: 22477
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: GFP (mut3b) (see part BBa_E0040) with added LVA-ssrA degradation tag. The tag increases GFP turn-over rate, thus providing better temporal resolution of green fluorescence. In the same time, maximal fluorescence amplitudes will be lower as newly formed protein is degraded as soon as it is formed. The tag encodes peptide sequence AANDENYALVA and is recognized by ClpA and ClpX unfoldases and ClpX mediator SspB.[1] ClpA and ClpX then form a proteosome-like complex with ClpP protease and the protein is degraded.[1] The final three residues of the tag determines the strength of interaction with ClpX and thus the final protein degradation rate.[2] The LVA tag is reported to lead to fast protein degradation, degrading GFP with rate -0.018 per minute.[2] However, be aware that exact protein degradation rate depends on multiple factors: ClpXP and ClpAP protease and SspB mediator concentrations, protein stability, Km of binding to the protease, temperature [3]. References [1] Flynn, J. M. et al. Overlapping recognition determinants within the ssrA degradation tag allow modulation of proteolysis. Proc. Natl. Acad. Sci. U. S. A. 98, 10584???9 (2001). [2] Andersen, J. B. et al. New unstable variants of green fluorescent protein for studies of transient gene expression in bacteria. Appl. Environ. Microbiol. 64, 2240???6 (1998). [3] Purcell, O., Grierson, C. S., Bernardo, M. Di & Savery, N. J. Temperature dependence of ssrA-tag mediated protein degradation. J. Biol. Eng. 6, 10 (2012).
mutable notes: The tag was attached to GFP using PCR and MABEL (mutagenesis with blunt-end ligation), thus avoiding introduction of additonal residues and restriction site. Different parts of the tag are recognized by different proteins, for example, the final 3 residues (LVA in this case) are recognised by ClpX, whereas first 4 residues of the tag are required for efficient SspB binding.[1] Thus modifications of these critical residues alter the efficacy with what different proteases bind to it.
mutable provenance: GFP comes from part E1010, the tag sequence was obtained from paper by Andersen et al., (1998).[2]
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: stop
sequence annotation: GFP (mut3b)
sequence annotation: start
sequence annotation: LVA
sequence: https://synbiohub.org/public/igem/BBa_K1399004_sequence/1

stop
type: SequenceAnnotation
persistent identity: annotation2383916
display id: annotation2383916
version: 1
top level: BBa_K1399004
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1399004/annotation2383916/range2383916/1

https://synbiohub.org/public/igem/BBa_K1399004/annotation2383916/range2383916/1
type: Range
persistent identity: range2383916
display id: range2383916
version: 1
direction: direction/forward
top level: BBa_K1399004
start: 748
end: 750
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2383917
display id: annotation2383917
version: 1
top level: BBa_K1399004
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1399004/annotation2383917/range2383917/1

https://synbiohub.org/public/igem/BBa_K1399004/annotation2383917/range2383917/1
type: Range
persistent identity: range2383917
display id: range2383917
version: 1
direction: direction/forward
top level: BBa_K1399004
start: 751
end: 753
orientation: inline

GFP (mut3b)
type: SequenceAnnotation
persistent identity: annotation2383914
display id: annotation2383914
version: 1
top level: BBa_K1399004
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1399004/annotation2383914/range2383914/1

https://synbiohub.org/public/igem/BBa_K1399004/annotation2383914/range2383914/1
type: Range
persistent identity: range2383914
display id: range2383914
version: 1
direction: direction/forward
top level: BBa_K1399004
start: 4
end: 714
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2383913
display id: annotation2383913
version: 1
top level: BBa_K1399004
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1399004/annotation2383913/range2383913/1

https://synbiohub.org/public/igem/BBa_K1399004/annotation2383913/range2383913/1
type: Range
persistent identity: range2383913
display id: range2383913
version: 1
direction: direction/forward
top level: BBa_K1399004
start: 1
end: 3
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation2383915
display id: annotation2383915
version: 1
top level: BBa_K1399004
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1399004/annotation2383915/range2383915/1

https://synbiohub.org/public/igem/BBa_K1399004/annotation2383915/range2383915/1
type: Range
persistent identity: range2383915
display id: range2383915
version: 1
direction: direction/forward
top level: BBa_K1399004
start: 715
end: 747
orientation: inline

BBa_K1362461
type: ComponentDefinition
persistent identity: BBa K1362461
display id: BBa_K1362461
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: **highly** engineered mutant of red fluorescent protein from Discosoma striata (coral) with barcode
creator: Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena Büscher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Schäfer, Carolin Schmelas, Silvan Schmitz, Max Waldha
created: 2014-10-07T11:00:00Z
modified: 2016-01-25T01:30:37Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1738_
m user id: 4206
owner id: 22951
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This part is essentially identical to BBa_E1010 , but has a point mutation in the barcode. Barcodes are discontinued, but composite parts using this part will have the mutated barcode.
mutable provenance: ''Discosoma striata''
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: mrfp1
sequence annotation: mutated barcode
sequence: https://synbiohub.org/public/igem/BBa_K1362461_sequence/1

mrfp1
type: SequenceAnnotation
persistent identity: annotation2404599
display id: annotation2404599
version: 1
top level: BBa_K1362461
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1362461/annotation2404599/range2404599/1

https://synbiohub.org/public/igem/BBa_K1362461/annotation2404599/range2404599/1
type: Range
persistent identity: range2404599
display id: range2404599
version: 1
direction: direction/forward
top level: BBa_K1362461
start: 1
end: 675
orientation: inline

mutated barcode
type: SequenceAnnotation
persistent identity: annotation2404604
display id: annotation2404604
version: 1
top level: BBa_K1362461
role: SO:0000807
role: feature/barcode
location: https://synbiohub.org/public/igem/BBa_K1362461/annotation2404604/range2404604/1

https://synbiohub.org/public/igem/BBa_K1362461/annotation2404604/range2404604/1
type: Range
persistent identity: range2404604
display id: range2404604
version: 1
direction: direction/forward
top level: BBa_K1362461
start: 682
end: 706
orientation: inline

eCFP
type: ComponentDefinition
persistent identity: BBa K678015
display id: BBa_K678015
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: eCFP-module
creator: DTU-Denmark-2
created: 2011-09-12T11:00:00Z
modified: 2015-05-08T01:13:04Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _882_
m user id: 0
owner id: 10556
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part was designed for the Plug'n'Play with DNA assembly standard (RFC 80), therefore this part contains tails at the 5' and 3' ends as shown on the figure below.
mutable notes: Ni
mutable provenance: The Danish Cancer Society
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: eCFP
sequence annotation: Linker 2
sequence annotation: Kozak
sequence: https://synbiohub.org/public/igem/BBa_K678015_sequence/1

eCFP
type: SequenceAnnotation
persistent identity: annotation2127709
display id: annotation2127709
version: 1
top level: eCFP
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K678015/annotation2127709/range2127709/1

https://synbiohub.org/public/igem/BBa_K678015/annotation2127709/range2127709/1
type: Range
persistent identity: range2127709
display id: range2127709
version: 1
direction: direction/forward
top level: eCFP
start: 16
end: 813
orientation: inline

Linker 2
type: SequenceAnnotation
persistent identity: annotation2127708
display id: annotation2127708
version: 1
top level: eCFP
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678015/annotation2127708/range2127708/1

https://synbiohub.org/public/igem/BBa_K678015/annotation2127708/range2127708/1
type: Range
persistent identity: range2127708
display id: range2127708
version: 1
direction: direction/forward
top level: eCFP
start: 1
end: 8
orientation: inline

Kozak
type: SequenceAnnotation
persistent identity: annotation2127786
display id: annotation2127786
version: 1
top level: eCFP
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678015/annotation2127786/range2127786/1

https://synbiohub.org/public/igem/BBa_K678015/annotation2127786/range2127786/1
type: Range
persistent identity: range2127786
display id: range2127786
version: 1
direction: direction/forward
top level: eCFP
start: 9
end: 19
orientation: inline

BBa_K192001
type: ComponentDefinition
persistent identity: BBa K192001
display id: BBa_K192001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CFP +tgt +lva
creator: Daniel Wong, Kenny Zhan, Jason An
created: 2009-09-14T11:00:00Z
modified: 2015-05-08T01:11:15Z
discontinued: false
dominant: false
experience: experience/None
group u list: _297_
m user id: 0
owner id: 2231
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: CFP with targeting sequence and LVA tag. The targeting sequence targets the protein to the encapsulin nanocompartment [1] with the aim of increasing the half-life of the protein. LVA should help limit the amount of background activity. [1] M. Sutter, et al., "Structural basis of enzyme encapsulation into a bacterial nanocompartment," Nat. Struct. & Mol. Biol., vol. 15, pp. 939-47., 2008.
mutable notes: We decided to position the LVA at the C-terminus of the construct since that is its natural position. However, the tgt extension sequence is also typically found at the C-terminus, so we're hoping that the shorter LVA sequence won't interfere too much with the binding with encapsulin. If necessary, it'll be possible to remove the LVA tag via PCR.
mutable provenance: Part was synthesized.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: lva
sequence annotation: double stop
sequence annotation: tgt
sequence annotation: cfp
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_K192001_sequence/1

lva
type: SequenceAnnotation
persistent identity: annotation2020963
display id: annotation2020963
version: 1
top level: BBa_K192001
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K192001/annotation2020963/range2020963/1

https://synbiohub.org/public/igem/BBa_K192001/annotation2020963/range2020963/1
type: Range
persistent identity: range2020963
display id: range2020963
version: 1
direction: direction/forward
top level: BBa_K192001
start: 820
end: 852
orientation: inline

double stop
type: SequenceAnnotation
persistent identity: annotation2020960
display id: annotation2020960
version: 1
top level: BBa_K192001
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K192001/annotation2020960/range2020960/1

https://synbiohub.org/public/igem/BBa_K192001/annotation2020960/range2020960/1
type: Range
persistent identity: range2020960
display id: range2020960
version: 1
direction: direction/forward
top level: BBa_K192001
start: 853
end: 858
orientation: inline

tgt
type: SequenceAnnotation
persistent identity: annotation2020962
display id: annotation2020962
version: 1
top level: BBa_K192001
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K192001/annotation2020962/range2020962/1

https://synbiohub.org/public/igem/BBa_K192001/annotation2020962/range2020962/1
type: Range
persistent identity: range2020962
display id: range2020962
version: 1
direction: direction/forward
top level: BBa_K192001
start: 718
end: 819
orientation: inline

cfp
type: SequenceAnnotation
persistent identity: annotation2020961
display id: annotation2020961
version: 1
top level: BBa_K192001
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K192001/annotation2020961/range2020961/1

https://synbiohub.org/public/igem/BBa_K192001/annotation2020961/range2020961/1
type: Range
persistent identity: range2020961
display id: range2020961
version: 1
direction: direction/forward
top level: BBa_K192001
start: 4
end: 717
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2020959
display id: annotation2020959
version: 1
top level: BBa_K192001
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K192001/annotation2020959/range2020959/1

https://synbiohub.org/public/igem/BBa_K192001/annotation2020959/range2020959/1
type: Range
persistent identity: range2020959
display id: range2020959
version: 1
direction: direction/forward
top level: BBa_K192001
start: 1
end: 3
orientation: inline

BBa_K1680021
type: ComponentDefinition
persistent identity: BBa K1680021
display id: BBa_K1680021
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Dronpa caged Cre with NLS
creator: Nicolai von Kuegelgen
created: 2015-09-16T11:00:00Z
modified: 2016-01-26T01:27:03Z
discontinued: false
dominant: false
group u list: _2098_
m user id: 4206
owner id: 26366
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Protein coding region for a fusion protein of Cre recombinase (BBa_K1680007) that is C- & N-terminal flanked with 145N Dronpa (BBa_K1680006) using a 6 AA linker as spacer (BBa_K1680000). The construct has a N-terminal NLS sequence (BBa_K1680004). In the expressed construct the two dronpa domains should form multimerize leading to an inactive Cre recombinase that is reversibly activatable through dronpa illumination (see Zhou et al 2012).
mutable notes: 3A Assembly using RFC25
mutable provenance: gBlock design by Team Tuebingen
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Dronpa
sequence annotation: Cre recombinase
sequence annotation: linker
sequence annotation: SV40 NLS
sequence: https://synbiohub.org/public/igem/BBa_K1680021_sequence/1

Dronpa
type: SequenceAnnotation
persistent identity: annotation2477378
display id: annotation2477378
version: 1
top level: BBa_K1680021
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1680021/annotation2477378/range2477378/1

https://synbiohub.org/public/igem/BBa_K1680021/annotation2477378/range2477378/1
type: Range
persistent identity: range2477378
display id: range2477378
version: 1
direction: direction/forward
top level: BBa_K1680021
start: 1765
end: 2433
orientation: inline

Cre recombinase
type: SequenceAnnotation
persistent identity: annotation2477376
display id: annotation2477376
version: 1
top level: BBa_K1680021
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1680021/annotation2477376/range2477376/1

https://synbiohub.org/public/igem/BBa_K1680021/annotation2477376/range2477376/1
type: Range
persistent identity: range2477376
display id: range2477376
version: 1
direction: direction/forward
top level: BBa_K1680021
start: 718
end: 1746
orientation: inline

linker
type: SequenceAnnotation
persistent identity: annotation2477375
display id: annotation2477375
version: 1
top level: BBa_K1680021
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1680021/annotation2477375/range2477375/1

https://synbiohub.org/public/igem/BBa_K1680021/annotation2477375/range2477375/1
type: Range
persistent identity: range2477375
display id: range2477375
version: 1
direction: direction/forward
top level: BBa_K1680021
start: 700
end: 717
orientation: inline

SV40 NLS
type: SequenceAnnotation
persistent identity: annotation2477373
display id: annotation2477373
version: 1
top level: BBa_K1680021
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1680021/annotation2477373/range2477373/1

https://synbiohub.org/public/igem/BBa_K1680021/annotation2477373/range2477373/1
type: Range
persistent identity: range2477373
display id: range2477373
version: 1
direction: direction/forward
top level: BBa_K1680021
start: 4
end: 24
orientation: inline

Dronpa
type: SequenceAnnotation
persistent identity: annotation2477374
display id: annotation2477374
version: 1
top level: BBa_K1680021
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1680021/annotation2477374/range2477374/1

https://synbiohub.org/public/igem/BBa_K1680021/annotation2477374/range2477374/1
type: Range
persistent identity: range2477374
display id: range2477374
version: 1
direction: direction/forward
top level: BBa_K1680021
start: 31
end: 699
orientation: inline

linker
type: SequenceAnnotation
persistent identity: annotation2477377
display id: annotation2477377
version: 1
top level: BBa_K1680021
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1680021/annotation2477377/range2477377/1

https://synbiohub.org/public/igem/BBa_K1680021/annotation2477377/range2477377/1
type: Range
persistent identity: range2477377
display id: range2477377
version: 1
direction: direction/forward
top level: BBa_K1680021
start: 1747
end: 1764
orientation: inline

BBa_K1673010
type: ComponentDefinition
persistent identity: BBa K1673010
display id: BBa_K1673010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Partially UV-Resistant RFP
creator: Jarrod Shilts
created: 2015-09-16T11:00:00Z
modified: 2016-01-21T04:17:27Z
discontinued: false
dominant: false
group u list: _2091_
m user id: 4206
owner id: 19415
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Partially optimized RFP sequence, based on E1010. Has a 4% reduction in the number of possible pyrimidine dimer-forming sites.
mutable notes: Sequence produced by the Vanderbilt iGEM 2015 mutation optimization software.
mutable provenance: Synthesized DNA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1673010_sequence/1

BBa_S05324
type: ComponentDefinition
persistent identity: BBa S05324
display id: BBa_S05324
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: J23101:K592024
creator: Trevor Y.H. Ho
created: 2016-04-11T11:00:00Z
modified: 2016-04-21T12:54:00Z
discontinued: true
dominant: false
experience: experience/None
group u list: _9_
m user id: 8758
owner id: 8758
owning group id: 9
status: status/Deleted
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_K592024
component: BBa_J23101
sequence annotation: BBa_K592024
sequence annotation: BBa_J23101
sequence: https://synbiohub.org/public/igem/BBa_S05324_sequence/1

BBa_K592024
type: Component
persistent identity: component2478626
display id: component2478626
version: 1
top level: BBa_S05324
access: public
definition: https://synbiohub.org/public/igem/BBa_K592024/1

BBa_J23101
type: Component
persistent identity: component2478621
display id: component2478621
version: 1
top level: BBa_S05324
access: public
definition: https://synbiohub.org/public/igem/BBa_J23101/1

BBa_K592024
type: SequenceAnnotation
persistent identity: annotation2478626
display id: annotation2478626
version: 1
top level: BBa_S05324
location: https://synbiohub.org/public/igem/BBa_S05324/annotation2478626/range2478626/1
component: BBa_K592024

https://synbiohub.org/public/igem/BBa_S05324/annotation2478626/range2478626/1
type: Range
persistent identity: range2478626
display id: range2478626
version: 1
direction: direction/reverse
top level: BBa_S05324
start: 44
end: 766
orientation: inline

BBa_J23101
type: SequenceAnnotation
persistent identity: annotation2478621
display id: annotation2478621
version: 1
top level: BBa_S05324
location: https://synbiohub.org/public/igem/BBa_S05324/annotation2478621/range2478621/1
component: BBa_J23101

https://synbiohub.org/public/igem/BBa_S05324/annotation2478621/range2478621/1
type: Range
persistent identity: range2478621
display id: range2478621
version: 1
direction: direction/reverse
top level: BBa_S05324
start: 1
end: 35
orientation: inline

BBa_K1486048
type: ComponentDefinition
persistent identity: BBa K1486048
display id: BBa_K1486048
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CpxR reporter
creator: EPFL iGem team 2014
created: 2014-10-06T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: Calgary's BBa_K339007 repaired (part didn't have the sequence they said they had.
mutable notes: to edit
mutable provenance: to edit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_K135000
component: BBa_I13507
sequence annotation: BBa_K135000
sequence annotation: BBa_I13507
sequence: https://synbiohub.org/public/igem/BBa_K1486048_sequence/1

BBa_K135000
type: Component
persistent identity: component2398411
display id: component2398411
version: 1
top level: BBa_K1486048
access: public
definition: https://synbiohub.org/public/igem/BBa_K135000/1

BBa_I13507
type: Component
persistent identity: component2398423
display id: component2398423
version: 1
top level: BBa_K1486048
access: public
definition: https://synbiohub.org/public/igem/BBa_I13507/1

BBa_K135000
type: SequenceAnnotation
persistent identity: annotation2398411
display id: annotation2398411
version: 1
top level: BBa_K1486048
location: https://synbiohub.org/public/igem/BBa_K1486048/annotation2398411/range2398411/1
component: BBa_K135000

https://synbiohub.org/public/igem/BBa_K1486048/annotation2398411/range2398411/1
type: Range
persistent identity: range2398411
display id: range2398411
version: 1
direction: direction/reverse
top level: BBa_K1486048
start: 1
end: 55
orientation: inline

BBa_I13507
type: SequenceAnnotation
persistent identity: annotation2398423
display id: annotation2398423
version: 1
top level: BBa_K1486048
location: https://synbiohub.org/public/igem/BBa_K1486048/annotation2398423/range2398423/1
component: BBa_I13507

https://synbiohub.org/public/igem/BBa_K1486048/annotation2398423/range2398423/1
type: Range
persistent identity: range2398423
display id: range2398423
version: 1
direction: direction/reverse
top level: BBa_K1486048
start: 64
end: 924
orientation: inline

BBa_K1673101
type: ComponentDefinition
persistent identity: BBa K1673101
display id: BBa_K1673101
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: UV-Resistant RFP
creator: Jarrod Shilts
created: 2015-09-16T11:00:00Z
modified: 2016-01-21T04:04:18Z
discontinued: false
dominant: false
group u list: _2091_
m user id: 4206
owner id: 19415
owning group id: 9
status: status/Planning
bookmark: false
mutable description: RFP sequence, based on E1010, with the number of possible pyrimidine dimer-forming sites reduced to the maximum possible extent.
mutable notes: Sequence produced by the Vanderbilt iGEM 2015 mutation optimization software.
mutable provenance: Synthesized DNA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1673101_sequence/1

BBa_K544021
type: ComponentDefinition
persistent identity: BBa K544021
display id: BBa_K544021
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: dKeima-Red
creator: Leung Man Hong
created: 2011-09-29T11:00:00Z
modified: 2015-05-08T01:12:39Z
discontinued: false
dominant: false
group u list: _711_
m user id: 0
owner id: 6780
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: the fluorescent protein ???Keima-Red,???
mutable notes: N/A
mutable provenance: From DNA synthesis.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: dKeima
sequence: https://synbiohub.org/public/igem/BBa_K544021_sequence/1

dKeima
type: SequenceAnnotation
persistent identity: annotation2154574
display id: annotation2154574
version: 1
top level: BBa_K544021
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K544021/annotation2154574/range2154574/1

https://synbiohub.org/public/igem/BBa_K544021/annotation2154574/range2154574/1
type: Range
persistent identity: range2154574
display id: range2154574
version: 1
direction: direction/forward
top level: BBa_K544021
start: 1
end: 669
orientation: inline

mCh
type: ComponentDefinition
persistent identity: BBa J176005
display id: BBa_J176005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: mCh
creator: Karmella Haynes
created: 2011-09-28T11:00:00Z
modified: 2015-08-31T04:08:33Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _863_
m user id: 0
owner id: 10865
owning group id: 9
sample status: In stock
sample status: It's complicated
status: status/Available
status: status/Planning
bookmark: false
mutable description: Humanized mCherry fluorescent protein
mutable notes: TBA
mutable provenance: TBA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence: https://synbiohub.org/public/igem/BBa_J176005_sequence/1

BBa_K1673103
type: ComponentDefinition
persistent identity: BBa K1673103
display id: BBa_K1673103
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: UV Mutation Maximizied RFP
creator: Jarrod Shilts
created: 2015-09-16T11:00:00Z
modified: 2016-01-21T04:03:48Z
discontinued: false
dominant: false
group u list: _2091_
m user id: 4206
owner id: 19415
owning group id: 9
status: status/Planning
bookmark: false
mutable description: RFP sequence, based on E1010, with the number of possible pyrimidine dimer-forming sites increased to the maximum possible amount.
mutable notes: Sequence produced by the Vanderbilt iGEM 2015 mutation optimization software
mutable provenance: Synthesized DNA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1673103_sequence/1

cfpx2
type: ComponentDefinition
persistent identity: BBa K165014
display id: BBa_K165014
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CFP x2
creator: John Szymanski
created: 2008-10-25T11:00:00Z
modified: 2015-05-08T01:10:55Z
discontinued: false
dominant: false
experience: experience/None
group u list: _267_
m user id: 0
owner id: 2512
owning group id: 58
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This is 2 copies of the CFP gene fused to each other. The duplex FP tends to be more stable, and is used in synthetic transcription factors to provide a fluorescent output of concentration as well as stability.
mutable notes: -
mutable provenance: From David Drubin in the Silver Lab at Harvard.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence: https://synbiohub.org/public/igem/BBa_K165014_sequence/1

BBa_K1680023
type: ComponentDefinition
persistent identity: BBa K1680023
display id: BBa_K1680023
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Dronpa caged Cre with NLS
creator: Nicolai von Kuegelgen
created: 2015-09-16T11:00:00Z
modified: 2016-01-26T10:42:40Z
discontinued: false
dominant: false
group u list: _2098_
m user id: 4206
owner id: 26366
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Protein coding region for a fusion protein of Cre recombinase (BBa_K1680007) that is C- & N-terminal flanked with 145N Dronpa (BBa_K1680006) using a 12 AA linker as spacer (BBa_K1680002). The construct has a N-terminal NLS sequence (BBa_K1680004). In the expressed construct the two dronpa domains should form multimerize leading to an inactive Cre recombinase that is reversibly activatable through dronpa illumination (see Zhou et al 2012).
mutable notes: 3A assembly using RFC25
mutable provenance: gBlock design by Team Tuebingen
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: K1680006
sequence annotation: K1680007
sequence annotation: K1680002
sequence annotation: K1680004
sequence: https://synbiohub.org/public/igem/BBa_K1680023_sequence/1

K1680006
type: SequenceAnnotation
persistent identity: annotation2477380
display id: annotation2477380
version: 1
top level: BBa_K1680023
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1680023/annotation2477380/range2477380/1

https://synbiohub.org/public/igem/BBa_K1680023/annotation2477380/range2477380/1
type: Range
persistent identity: range2477380
display id: range2477380
version: 1
direction: direction/forward
top level: BBa_K1680023
start: 31
end: 699
orientation: inline

K1680007
type: SequenceAnnotation
persistent identity: annotation2477382
display id: annotation2477382
version: 1
top level: BBa_K1680023
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1680023/annotation2477382/range2477382/1

https://synbiohub.org/public/igem/BBa_K1680023/annotation2477382/range2477382/1
type: Range
persistent identity: range2477382
display id: range2477382
version: 1
direction: direction/forward
top level: BBa_K1680023
start: 736
end: 1764
orientation: inline

K1680002
type: SequenceAnnotation
persistent identity: annotation2477384
display id: annotation2477384
version: 1
top level: BBa_K1680023
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1680023/annotation2477384/range2477384/1

https://synbiohub.org/public/igem/BBa_K1680023/annotation2477384/range2477384/1
type: Range
persistent identity: range2477384
display id: range2477384
version: 1
direction: direction/forward
top level: BBa_K1680023
start: 1765
end: 1800
orientation: inline

K1680006
type: SequenceAnnotation
persistent identity: annotation2477381
display id: annotation2477381
version: 1
top level: BBa_K1680023
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1680023/annotation2477381/range2477381/1

https://synbiohub.org/public/igem/BBa_K1680023/annotation2477381/range2477381/1
type: Range
persistent identity: range2477381
display id: range2477381
version: 1
direction: direction/forward
top level: BBa_K1680023
start: 1801
end: 2469
orientation: inline

K1680004
type: SequenceAnnotation
persistent identity: annotation2477379
display id: annotation2477379
version: 1
top level: BBa_K1680023
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1680023/annotation2477379/range2477379/1

https://synbiohub.org/public/igem/BBa_K1680023/annotation2477379/range2477379/1
type: Range
persistent identity: range2477379
display id: range2477379
version: 1
direction: direction/forward
top level: BBa_K1680023
start: 1
end: 24
orientation: inline

K1680002
type: SequenceAnnotation
persistent identity: annotation2477383
display id: annotation2477383
version: 1
top level: BBa_K1680023
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1680023/annotation2477383/range2477383/1

https://synbiohub.org/public/igem/BBa_K1680023/annotation2477383/range2477383/1
type: Range
persistent identity: range2477383
display id: range2477383
version: 1
direction: direction/forward
top level: BBa_K1680023
start: 700
end: 735
orientation: inline

BBa_K1033915
type: ComponentDefinition
persistent identity: BBa K1033915
display id: BBa_K1033915
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: amajLime, yellow-green chromoprotein (incl RBS)
creator: Erik Gullberg
created: 2013-09-16T11:00:00Z
modified: 2015-05-08T01:08:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1340_
m user id: 0
owner id: 13997
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This chromoprotein from the coral Anemonia majano, amajLime, naturally exhibits strong color when expressed.
mutable notes: Synthesized and codon optimized for E coli by GenScript.
mutable provenance: Anemonia majano
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_B0034
component: BBa_K1033916
sequence annotation: BBa_K1033916
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K1033915_sequence/1

BBa_B0034
type: Component
persistent identity: component2350100
display id: component2350100
version: 1
top level: BBa_K1033915
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K1033916
type: Component
persistent identity: component2350102
display id: component2350102
version: 1
top level: BBa_K1033915
access: public
definition: BBa_K1033916

BBa_K1033916
type: SequenceAnnotation
persistent identity: annotation2350102
display id: annotation2350102
version: 1
top level: BBa_K1033915
location: https://synbiohub.org/public/igem/BBa_K1033915/annotation2350102/range2350102/1
component: BBa_K1033916

https://synbiohub.org/public/igem/BBa_K1033915/annotation2350102/range2350102/1
type: Range
persistent identity: range2350102
display id: range2350102
version: 1
direction: direction/reverse
top level: BBa_K1033915
start: 19
end: 711
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2350100
display id: annotation2350100
version: 1
top level: BBa_K1033915
location: https://synbiohub.org/public/igem/BBa_K1033915/annotation2350100/range2350100/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1033915/annotation2350100/range2350100/1
type: Range
persistent identity: range2350100
display id: range2350100
version: 1
direction: direction/reverse
top level: BBa_K1033915
start: 1
end: 12
orientation: inline

BBa_K784006
type: ComponentDefinition
persistent identity: BBa K784006
display id: BBa_K784006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Theophylline riboswitch 12.1 + mCherry
creator: Ilya Vainberg Slutskin
created: 2012-09-12T11:00:00Z
modified: 2015-05-08T01:13:21Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _1039_
m user id: 0
owner id: 11677
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This part is a direct fusion of the theophylline riboswitch (BBa_K784005) and the mCherry protein (BBa_J06504) which was amplified from BBa_J06702. The part was generated by [http://openwetware.org/wiki/Assembly_pcr Assembly_pcr]. This part was to be used in order to characterize the effect of theophylline concentration on the level of translation of the mCherry protein.
mutable notes: The theophylline riboswitch had to be directly fused to the start codon of the mCherry protein. To achieve this, [http://openwetware.org/wiki/Assembly_pcr Assembly_pcr] was used.
mutable provenance: This part is a composite part of BBa_K784005 and BBa_J06504
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_J06504
component: BBa_K784005
sequence annotation: BBa_J06504
sequence annotation: BBa_K784005
sequence: https://synbiohub.org/public/igem/BBa_K784006_sequence/1

BBa_J06504
type: Component
persistent identity: component2182910
display id: component2182910
version: 1
top level: BBa_K784006
access: public
definition: mCherry

BBa_K784005
type: Component
persistent identity: component2182907
display id: component2182907
version: 1
top level: BBa_K784006
access: public
definition: https://synbiohub.org/public/igem/BBa_K784005/1

BBa_J06504
type: SequenceAnnotation
persistent identity: annotation2182910
display id: annotation2182910
version: 1
top level: BBa_K784006
location: https://synbiohub.org/public/igem/BBa_K784006/annotation2182910/range2182910/1
component: BBa_J06504

https://synbiohub.org/public/igem/BBa_K784006/annotation2182910/range2182910/1
type: Range
persistent identity: range2182910
display id: range2182910
version: 1
direction: direction/reverse
top level: BBa_K784006
start: 75
end: 788
orientation: inline

BBa_K784005
type: SequenceAnnotation
persistent identity: annotation2182907
display id: annotation2182907
version: 1
top level: BBa_K784006
location: https://synbiohub.org/public/igem/BBa_K784006/annotation2182907/range2182907/1
component: BBa_K784005

https://synbiohub.org/public/igem/BBa_K784006/annotation2182907/range2182907/1
type: Range
persistent identity: range2182907
display id: range2182907
version: 1
direction: direction/reverse
top level: BBa_K784006
start: 1
end: 74
orientation: inline

BBa_K1072018
type: ComponentDefinition
persistent identity: BBa K1072018
display id: BBa_K1072018
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pGAL1 + Flag + Brho + odr-10 + GFP + ADH1Te + pFUS1 + BFP + ADH1Te
creator: Junqi kuang
created: 2013-09-21T11:00:00Z
modified: 2015-05-08T01:09:01Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1381_
m user id: 0
owner id: 16002
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: -
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1072014
component: BBa_K1072020
sequence annotation: BBa_K1072020
sequence annotation: BBa_K1072014
sequence: https://synbiohub.org/public/igem/BBa_K1072018_sequence/1

BBa_K1072014
type: Component
persistent identity: component2366672
display id: component2366672
version: 1
top level: BBa_K1072018
access: public
definition: https://synbiohub.org/public/igem/BBa_K1072014/1

BBa_K1072020
type: Component
persistent identity: component2366679
display id: component2366679
version: 1
top level: BBa_K1072018
access: public
definition: https://synbiohub.org/public/igem/BBa_K1072020/1

BBa_K1072020
type: SequenceAnnotation
persistent identity: annotation2366679
display id: annotation2366679
version: 1
top level: BBa_K1072018
location: https://synbiohub.org/public/igem/BBa_K1072018/annotation2366679/range2366679/1
component: BBa_K1072020

https://synbiohub.org/public/igem/BBa_K1072018/annotation2366679/range2366679/1
type: Range
persistent identity: range2366679
display id: range2366679
version: 1
direction: direction/reverse
top level: BBa_K1072018
start: 2766
end: 4235
orientation: inline

BBa_K1072014
type: SequenceAnnotation
persistent identity: annotation2366672
display id: annotation2366672
version: 1
top level: BBa_K1072018
location: https://synbiohub.org/public/igem/BBa_K1072018/annotation2366672/range2366672/1
component: BBa_K1072014

https://synbiohub.org/public/igem/BBa_K1072018/annotation2366672/range2366672/1
type: Range
persistent identity: range2366672
display id: range2366672
version: 1
direction: direction/reverse
top level: BBa_K1072018
start: 1
end: 2757
orientation: inline

BBa_K780004
type: ComponentDefinition
persistent identity: BBa K780004
display id: BBa_K780004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS+GFP for expression in Bacillus subtilis
creator: iGEM 2012 Warsaw Team
created: 2012-09-25T11:00:00Z
modified: 2015-05-08T01:13:18Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1034_
m user id: 0
owner id: 10113
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: It is succesfully joined B.subtilis RBS with GFP reporter protein. Can be used to measure strenght of B.subtilis promoters. In order to work with it you must first add appropriate terminator at the end. We recommend BBa_K780000 - a terminator designed for use with B.subtilis.
mutable notes: -
mutable provenance: RBS was taken from 'host-takover module' from B.subtilis Bacteriophage SPO1 and GPF is derived from jellyfish Aequeora victoria (BBa_E0040).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K780002
component: BBa_E0040
sequence annotation: BBa_K780002
sequence annotation: BBa_E0040
sequence: https://synbiohub.org/public/igem/BBa_K780004_sequence/1

BBa_K780002
type: Component
persistent identity: component2200424
display id: component2200424
version: 1
top level: BBa_K780004
access: public
definition: https://synbiohub.org/public/igem/BBa_K780002/1

BBa_E0040
type: Component
persistent identity: component2200426
display id: component2200426
version: 1
top level: BBa_K780004
access: public
definition: GFP

BBa_K780002
type: SequenceAnnotation
persistent identity: annotation2200424
display id: annotation2200424
version: 1
top level: BBa_K780004
location: https://synbiohub.org/public/igem/BBa_K780004/annotation2200424/range2200424/1
component: BBa_K780002

https://synbiohub.org/public/igem/BBa_K780004/annotation2200424/range2200424/1
type: Range
persistent identity: range2200424
display id: range2200424
version: 1
direction: direction/reverse
top level: BBa_K780004
start: 1
end: 16
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation2200426
display id: annotation2200426
version: 1
top level: BBa_K780004
location: https://synbiohub.org/public/igem/BBa_K780004/annotation2200426/range2200426/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_K780004/annotation2200426/range2200426/1
type: Range
persistent identity: range2200426
display id: range2200426
version: 1
direction: direction/reverse
top level: BBa_K780004
start: 23
end: 742
orientation: inline

BBa_K1399017
type: ComponentDefinition
persistent identity: BBa K1399017
display id: BBa_K1399017
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Plac-GFP(DAS+2)
creator: Anna Stikane
created: 2014-09-18T11:00:00Z
modified: 2015-05-08T01:10:16Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1777_
m user id: 0
owner id: 22477
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: Lactose/IPTG inducible promoter with GFP reporter tagged with NYADAS-ssrA degradation tag followed by terminator. The tagged GFP is actively degraded within cell, thus provides better temporal resolution of green fluorescence and promoter activity.
mutable notes: Part was assembled using BrickClip assembly (BBF RFC104) using other biobrick parts as templates. BrickClip assembly is a special case of more general Paperclip assembly method.[1]
mutable provenance: other biobricks
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_B0015
component: BBa_K1399007
component: BBa_B0034
component: BBa_R0010
sequence annotation: BBa_R0010
sequence annotation: BBa_B0015
sequence annotation: BBa_K1399007
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K1399017_sequence/1

BBa_B0015
type: Component
persistent identity: component2384196
display id: component2384196
version: 1
top level: BBa_K1399017
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_K1399007
type: Component
persistent identity: component2384189
display id: component2384189
version: 1
top level: BBa_K1399017
access: public
definition: BBa_K1399007

BBa_B0034
type: Component
persistent identity: component2384183
display id: component2384183
version: 1
top level: BBa_K1399017
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_R0010
type: Component
persistent identity: component2384175
display id: component2384175
version: 1
top level: BBa_K1399017
access: public
definition: https://synbiohub.org/public/igem/BBa_R0010/1

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation2384175
display id: annotation2384175
version: 1
top level: BBa_K1399017
location: https://synbiohub.org/public/igem/BBa_K1399017/annotation2384175/range2384175/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_K1399017/annotation2384175/range2384175/1
type: Range
persistent identity: range2384175
display id: range2384175
version: 1
direction: direction/forward
top level: BBa_K1399017
start: 1
end: 200
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2384196
display id: annotation2384196
version: 1
top level: BBa_K1399017
location: https://synbiohub.org/public/igem/BBa_K1399017/annotation2384196/range2384196/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K1399017/annotation2384196/range2384196/1
type: Range
persistent identity: range2384196
display id: range2384196
version: 1
direction: direction/reverse
top level: BBa_K1399017
start: 994
end: 1122
orientation: inline

BBa_K1399007
type: SequenceAnnotation
persistent identity: annotation2384189
display id: annotation2384189
version: 1
top level: BBa_K1399017
location: https://synbiohub.org/public/igem/BBa_K1399017/annotation2384189/range2384189/1
component: BBa_K1399007

https://synbiohub.org/public/igem/BBa_K1399017/annotation2384189/range2384189/1
type: Range
persistent identity: range2384189
display id: range2384189
version: 1
direction: direction/reverse
top level: BBa_K1399017
start: 227
end: 985
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2384183
display id: annotation2384183
version: 1
top level: BBa_K1399017
location: https://synbiohub.org/public/igem/BBa_K1399017/annotation2384183/range2384183/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1399017/annotation2384183/range2384183/1
type: Range
persistent identity: range2384183
display id: range2384183
version: 1
direction: direction/reverse
top level: BBa_K1399017
start: 209
end: 220
orientation: inline

BBa_K1031521
type: ComponentDefinition
persistent identity: BBa K1031521
display id: BBa_K1031521
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Pg-B0032-sfGFP-Terminator (HpaR)
creator: He Shuaixin
created: 2013-09-08T11:00:00Z
modified: 2015-05-08T01:08:47Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1338_
m user id: 0
owner id: 15843
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Pg-32-sfGFP-TT (HpaR)
mutable notes: molecular cloning
mutable provenance: synthesis, lab made
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: B0032
sequence annotation: B0015
sequence annotation: Pg (HpaR)
sequence annotation: sfGFP
sequence: https://synbiohub.org/public/igem/BBa_K1031521_sequence/1

B0032
type: SequenceAnnotation
persistent identity: annotation2362522
display id: annotation2362522
version: 1
top level: BBa_K1031521
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1031521/annotation2362522/range2362522/1

https://synbiohub.org/public/igem/BBa_K1031521/annotation2362522/range2362522/1
type: Range
persistent identity: range2362522
display id: range2362522
version: 1
direction: direction/forward
top level: BBa_K1031521
start: 234
end: 246
orientation: inline

B0015
type: SequenceAnnotation
persistent identity: annotation2362524
display id: annotation2362524
version: 1
top level: BBa_K1031521
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1031521/annotation2362524/range2362524/1

https://synbiohub.org/public/igem/BBa_K1031521/annotation2362524/range2362524/1
type: Range
persistent identity: range2362524
display id: range2362524
version: 1
direction: direction/forward
top level: BBa_K1031521
start: 982
end: 1110
orientation: inline

Pg (HpaR)
type: SequenceAnnotation
persistent identity: annotation2362521
display id: annotation2362521
version: 1
top level: BBa_K1031521
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1031521/annotation2362521/range2362521/1

https://synbiohub.org/public/igem/BBa_K1031521/annotation2362521/range2362521/1
type: Range
persistent identity: range2362521
display id: range2362521
version: 1
direction: direction/forward
top level: BBa_K1031521
start: 1
end: 227
orientation: inline

sfGFP
type: SequenceAnnotation
persistent identity: annotation2362523
display id: annotation2362523
version: 1
top level: BBa_K1031521
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1031521/annotation2362523/range2362523/1

https://synbiohub.org/public/igem/BBa_K1031521/annotation2362523/range2362523/1
type: Range
persistent identity: range2362523
display id: range2362523
version: 1
direction: direction/forward
top level: BBa_K1031521
start: 253
end: 975
orientation: inline

BBa_K1418030
type: ComponentDefinition
persistent identity: BBa K1418030
display id: BBa_K1418030
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: SYFP2
creator: Cody Maxfield
created: 2014-10-07T11:00:00Z
modified: 2016-01-26T02:11:39Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1796_
m user id: 4206
owner id: 13965
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: SYFP2
mutable notes: RFC 23
mutable provenance: SYFP2
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: atg
sequence annotation: SYFP2
sequence annotation: taataa
sequence: https://synbiohub.org/public/igem/BBa_K1418030_sequence/1

atg
type: SequenceAnnotation
persistent identity: annotation2421320
display id: annotation2421320
version: 1
top level: BBa_K1418030
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1418030/annotation2421320/range2421320/1

https://synbiohub.org/public/igem/BBa_K1418030/annotation2421320/range2421320/1
type: Range
persistent identity: range2421320
display id: range2421320
version: 1
direction: direction/forward
top level: BBa_K1418030
start: 1
end: 3
orientation: inline

SYFP2
type: SequenceAnnotation
persistent identity: annotation2421321
display id: annotation2421321
version: 1
top level: BBa_K1418030
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1418030/annotation2421321/range2421321/1

https://synbiohub.org/public/igem/BBa_K1418030/annotation2421321/range2421321/1
type: Range
persistent identity: range2421321
display id: range2421321
version: 1
direction: direction/forward
top level: BBa_K1418030
start: 4
end: 717
orientation: inline

taataa
type: SequenceAnnotation
persistent identity: annotation2421322
display id: annotation2421322
version: 1
top level: BBa_K1418030
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1418030/annotation2421322/range2421322/1

https://synbiohub.org/public/igem/BBa_K1418030/annotation2421322/range2421322/1
type: Range
persistent identity: range2421322
display id: range2421322
version: 1
direction: direction/forward
top level: BBa_K1418030
start: 718
end: 723
orientation: inline

BBa_K598001
type: ComponentDefinition
persistent identity: BBa K598001
display id: BBa_K598001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Theophylline Responsive Riboswitch 1G1 with Engineered RBS+GFP generator
creator: Siyang HAO, Tong MU, Zheng LUO, Mengqi ZHANG
created: 2011-09-26T11:00:00Z
modified: 2015-05-08T01:12:50Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _770_
m user id: 0
owner id: 8713
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: This is a GFP generator with a theophylline responsive riboswitch sequence. The mRNA with a theophylline riboswitch in it would respond to theophylline concerntration, producing different amount of GFP. Under the control of different promoters, it could be used to demonstrate the theophylline responsive curve, providing data for modeling. The theophylline riboswitch sequence could also be used to regulate the translation of other genes.
mutable notes: The RBS of this device is altered into AGGAGGU. When changing the RBS, the anti-RBS sequence should be changed as well.
mutable provenance: Theophylline riboswich is composed of a synthesized theophylline aptamer and a regulatory platform.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: stop
sequence annotation: BBa_B0010
sequence annotation: T7 TE
sequence annotation: BBa_E0040
sequence annotation: BBa_B0012
sequence annotation: polya
sequence annotation: Engineered RBS
sequence annotation: Theophylline responsive riboswitch Theo 1G1
sequence annotation: stem_loop
sequence annotation: eGFP
sequence: https://synbiohub.org/public/igem/BBa_K598001_sequence/1

stop
type: SequenceAnnotation
persistent identity: annotation2152892
display id: annotation2152892
version: 1
top level: BBa_K598001
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K598001/annotation2152892/range2152892/1

https://synbiohub.org/public/igem/BBa_K598001/annotation2152892/range2152892/1
type: Range
persistent identity: range2152892
display id: range2152892
version: 1
direction: direction/forward
top level: BBa_K598001
start: 936
end: 936
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2152882
display id: annotation2152882
version: 1
top level: BBa_K598001
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K598001/annotation2152882/range2152882/1

https://synbiohub.org/public/igem/BBa_K598001/annotation2152882/range2152882/1
type: Range
persistent identity: range2152882
display id: range2152882
version: 1
direction: direction/forward
top level: BBa_K598001
start: 815
end: 894
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation2152901
display id: annotation2152901
version: 1
top level: BBa_K598001
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K598001/annotation2152901/range2152901/1

https://synbiohub.org/public/igem/BBa_K598001/annotation2152901/range2152901/1
type: Range
persistent identity: range2152901
display id: range2152901
version: 1
direction: direction/forward
top level: BBa_K598001
start: 910
end: 929
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation2152703
display id: annotation2152703
version: 1
top level: BBa_K598001
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K598001/annotation2152703/range2152703/1

https://synbiohub.org/public/igem/BBa_K598001/annotation2152703/range2152703/1
type: Range
persistent identity: range2152703
display id: range2152703
version: 1
direction: direction/forward
top level: BBa_K598001
start: 87
end: 806
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2152705
display id: annotation2152705
version: 1
top level: BBa_K598001
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K598001/annotation2152705/range2152705/1

https://synbiohub.org/public/igem/BBa_K598001/annotation2152705/range2152705/1
type: Range
persistent identity: range2152705
display id: range2152705
version: 1
direction: direction/forward
top level: BBa_K598001
start: 903
end: 943
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation2152887
display id: annotation2152887
version: 1
top level: BBa_K598001
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_K598001/annotation2152887/range2152887/1

https://synbiohub.org/public/igem/BBa_K598001/annotation2152887/range2152887/1
type: Range
persistent identity: range2152887
display id: range2152887
version: 1
direction: direction/forward
top level: BBa_K598001
start: 930
end: 943
orientation: inline

Engineered RBS
type: SequenceAnnotation
persistent identity: annotation2152702
display id: annotation2152702
version: 1
top level: BBa_K598001
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K598001/annotation2152702/range2152702/1

https://synbiohub.org/public/igem/BBa_K598001/annotation2152702/range2152702/1
type: Range
persistent identity: range2152702
display id: range2152702
version: 1
direction: direction/forward
top level: BBa_K598001
start: 75
end: 81
orientation: inline

Theophylline responsive riboswitch Theo 1G1
type: SequenceAnnotation
persistent identity: annotation2152701
display id: annotation2152701
version: 1
top level: BBa_K598001
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K598001/annotation2152701/range2152701/1

https://synbiohub.org/public/igem/BBa_K598001/annotation2152701/range2152701/1
type: Range
persistent identity: range2152701
display id: range2152701
version: 1
direction: direction/forward
top level: BBa_K598001
start: 1
end: 86
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation2152895
display id: annotation2152895
version: 1
top level: BBa_K598001
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K598001/annotation2152895/range2152895/1

https://synbiohub.org/public/igem/BBa_K598001/annotation2152895/range2152895/1
type: Range
persistent identity: range2152895
display id: range2152895
version: 1
direction: direction/forward
top level: BBa_K598001
start: 826
end: 869
orientation: inline

eGFP
type: SequenceAnnotation
persistent identity: annotation2152704
display id: annotation2152704
version: 1
top level: BBa_K598001
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K598001/annotation2152704/range2152704/1

https://synbiohub.org/public/igem/BBa_K598001/annotation2152704/range2152704/1
type: Range
persistent identity: range2152704
display id: range2152704
version: 1
direction: direction/forward
top level: BBa_K598001
start: 87
end: 806
orientation: inline

BBa_K1399019
type: ComponentDefinition
persistent identity: BBa K1399019
display id: BBa_K1399019
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ptet-GFP(AAV)
creator: Anna Stikane
created: 2014-09-18T11:00:00Z
modified: 2015-05-08T01:10:16Z
discontinued: false
dominant: false
group u list: _1777_
m user id: 0
owner id: 22477
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: Tetracycline/aTc inducible promoter with GFP reporter tagged with AAV-ssrA degradation tag followed by terminator. The tagged GFP is actively degraded within cell, thus provides better temporal resolution of green fluorescence and promoter activity.
mutable notes: Part was assembled using BrickClip assembly (BBF RFC104) using other biobrick parts as templates. BrickClip assembly is a special case of more general Paperclip assembly method.[1]
mutable provenance: other biobricks
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_K1399005
component: BBa_B0034
component: BBa_B0015
component: BBa_R0040
sequence annotation: BBa_K1399005
sequence annotation: BBa_R0040
sequence annotation: BBa_B0034
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_K1399019_sequence/1

BBa_K1399005
type: Component
persistent identity: component2384231
display id: component2384231
version: 1
top level: BBa_K1399019
access: public
definition: BBa_K1399005

BBa_B0034
type: Component
persistent identity: component2384225
display id: component2384225
version: 1
top level: BBa_K1399019
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0015
type: Component
persistent identity: component2384238
display id: component2384238
version: 1
top level: BBa_K1399019
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_R0040
type: Component
persistent identity: component2384219
display id: component2384219
version: 1
top level: BBa_K1399019
access: public
definition: https://synbiohub.org/public/igem/BBa_R0040/1

BBa_K1399005
type: SequenceAnnotation
persistent identity: annotation2384231
display id: annotation2384231
version: 1
top level: BBa_K1399019
location: https://synbiohub.org/public/igem/BBa_K1399019/annotation2384231/range2384231/1
component: BBa_K1399005

https://synbiohub.org/public/igem/BBa_K1399019/annotation2384231/range2384231/1
type: Range
persistent identity: range2384231
display id: range2384231
version: 1
direction: direction/reverse
top level: BBa_K1399019
start: 81
end: 833
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2384219
display id: annotation2384219
version: 1
top level: BBa_K1399019
location: https://synbiohub.org/public/igem/BBa_K1399019/annotation2384219/range2384219/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_K1399019/annotation2384219/range2384219/1
type: Range
persistent identity: range2384219
display id: range2384219
version: 1
direction: direction/forward
top level: BBa_K1399019
start: 1
end: 54
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2384225
display id: annotation2384225
version: 1
top level: BBa_K1399019
location: https://synbiohub.org/public/igem/BBa_K1399019/annotation2384225/range2384225/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1399019/annotation2384225/range2384225/1
type: Range
persistent identity: range2384225
display id: range2384225
version: 1
direction: direction/reverse
top level: BBa_K1399019
start: 63
end: 74
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2384238
display id: annotation2384238
version: 1
top level: BBa_K1399019
location: https://synbiohub.org/public/igem/BBa_K1399019/annotation2384238/range2384238/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K1399019/annotation2384238/range2384238/1
type: Range
persistent identity: range2384238
display id: range2384238
version: 1
direction: direction/reverse
top level: BBa_K1399019
start: 842
end: 970
orientation: inline

BBa_K1031221
type: ComponentDefinition
persistent identity: BBa K1031221
display id: BBa_K1031221
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Po-B0031-sfGFP-Terminator (DmpR)
creator: He Shuaixin
created: 2013-09-08T11:00:00Z
modified: 2015-05-08T01:08:47Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1338_
m user id: 0
owner id: 15843
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: Po-31-sfGFP-TT (DmpR)
mutable notes: molecular cloning
mutable provenance: synthesis, lab made
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: B0015
sequence annotation: sfGFP
sequence annotation: B0031
sequence annotation: Po promoter
sequence: https://synbiohub.org/public/igem/BBa_K1031221_sequence/1

B0015
type: SequenceAnnotation
persistent identity: annotation2354353
display id: annotation2354353
version: 1
top level: BBa_K1031221
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1031221/annotation2354353/range2354353/1

https://synbiohub.org/public/igem/BBa_K1031221/annotation2354353/range2354353/1
type: Range
persistent identity: range2354353
display id: range2354353
version: 1
direction: direction/forward
top level: BBa_K1031221
start: 931
end: 1059
orientation: inline

sfGFP
type: SequenceAnnotation
persistent identity: annotation2354352
display id: annotation2354352
version: 1
top level: BBa_K1031221
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1031221/annotation2354352/range2354352/1

https://synbiohub.org/public/igem/BBa_K1031221/annotation2354352/range2354352/1
type: Range
persistent identity: range2354352
display id: range2354352
version: 1
direction: direction/forward
top level: BBa_K1031221
start: 200
end: 922
orientation: inline

B0031
type: SequenceAnnotation
persistent identity: annotation2354351
display id: annotation2354351
version: 1
top level: BBa_K1031221
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1031221/annotation2354351/range2354351/1

https://synbiohub.org/public/igem/BBa_K1031221/annotation2354351/range2354351/1
type: Range
persistent identity: range2354351
display id: range2354351
version: 1
direction: direction/forward
top level: BBa_K1031221
start: 180
end: 193
orientation: inline

Po promoter
type: SequenceAnnotation
persistent identity: annotation2354350
display id: annotation2354350
version: 1
top level: BBa_K1031221
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1031221/annotation2354350/range2354350/1

https://synbiohub.org/public/igem/BBa_K1031221/annotation2354350/range2354350/1
type: Range
persistent identity: range2354350
display id: range2354350
version: 1
direction: direction/forward
top level: BBa_K1031221
start: 1
end: 171
orientation: inline

fwYellow
type: ComponentDefinition
persistent identity: BBa K1033909
display id: BBa_K1033909
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: fwYellow, yellow chromoprotein (incl RBS)
creator: Erik Gullberg
created: 2013-09-16T11:00:00Z
modified: 2015-05-08T01:08:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1340_
m user id: 0
owner id: 13997
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This chromoprotein naturally exhibits strong color when expressed.
mutable notes: Cloned from FezziwigYFP vector, DNA 2.0.
mutable provenance: Synthetic chromoprotein from DNA 2.0.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: fwYellow
sequence annotation: BBa_K1033910
sequence annotation: rbs
sequence: https://synbiohub.org/public/igem/BBa_K1033909_sequence/1

fwYellow
type: SequenceAnnotation
persistent identity: annotation2350072
display id: annotation2350072
version: 1
top level: fwYellow
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1033909/annotation2350072/range2350072/1

https://synbiohub.org/public/igem/BBa_K1033909/annotation2350072/range2350072/1
type: Range
persistent identity: range2350072
display id: range2350072
version: 1
direction: direction/forward
top level: fwYellow
start: 19
end: 732
orientation: inline

BBa_K1033910
type: SequenceAnnotation
persistent identity: annotation2350071
display id: annotation2350071
version: 1
top level: fwYellow
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1033909/annotation2350071/range2350071/1

https://synbiohub.org/public/igem/BBa_K1033909/annotation2350071/range2350071/1
type: Range
persistent identity: range2350071
display id: range2350071
version: 1
direction: direction/forward
top level: fwYellow
start: 19
end: 732
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation2350059
display id: annotation2350059
version: 1
top level: fwYellow
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1033909/annotation2350059/range2350059/1

https://synbiohub.org/public/igem/BBa_K1033909/annotation2350059/range2350059/1
type: Range
persistent identity: range2350059
display id: range2350059
version: 1
direction: direction/forward
top level: fwYellow
start: 1
end: 12
orientation: inline

BBa_K772009
type: ComponentDefinition
persistent identity: BBa K772009
display id: BBa_K772009
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: GFP - LasR protein complex with flag tags
creator: Mustafa Elitok
created: 2012-09-25T11:00:00Z
modified: 2015-05-08T01:13:15Z
discontinued: false
dominant: false
group u list: _1024_
m user id: 0
owner id: 9573
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This part contains a green fluorescent protein (GFP) as a non-reactive chemical compound (anchor) in order to neutralize the active reactive fixed to it, an inducer regulatory protein (LasR is responsible to induce pLas promoter in the presence of 6HSLO3 which is a chemical compound sythesized by an enzyme that LasI gene codes.) and a TEV protease cleavage site between them to assemble these two proteins. BBa_772001 and BBa_K772002 are assembled to be used as the trigger of detection in the whole system which ends with the intracellular transfection (or activation) of TEV protease. After the activation, it is planned that TEV protease will cleave this anchor-inducer protein complex to release free the reporter. In conclusion, in this system, the inducer would activate a transcription pathway on the targeted inducible gene or the reporter would indicate that there is a change in the whole system. This part contains flag tags extra from BBa_K772006 in the GFP region; because it is planned to be used in the Co-IP/SDS-PAGE assay to be isolated.
mutable notes: Note that there is an RBS sequence just before the protein sequence in order to eliminate the need of addition of it.
mutable provenance: Cleavage site, LasR and GFP sequences are gathered from Registry.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
sequence annotation: TEV cleavage site
sequence annotation: Double terminator
sequence annotation: RBS
sequence annotation: LasR
sequence annotation: Flag tag
sequence annotation: GFP
sequence annotation: Anchor-inducer + flag
sequence: https://synbiohub.org/public/igem/BBa_K772009_sequence/1

TEV cleavage site
type: SequenceAnnotation
persistent identity: annotation2203920
display id: annotation2203920
version: 1
top level: BBa_K772009
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K772009/annotation2203920/range2203920/1

https://synbiohub.org/public/igem/BBa_K772009/annotation2203920/range2203920/1
type: Range
persistent identity: range2203920
display id: range2203920
version: 1
direction: direction/forward
top level: BBa_K772009
start: 768
end: 857
orientation: inline

Double terminator
type: SequenceAnnotation
persistent identity: annotation2203922
display id: annotation2203922
version: 1
top level: BBa_K772009
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K772009/annotation2203922/range2203922/1

https://synbiohub.org/public/igem/BBa_K772009/annotation2203922/range2203922/1
type: Range
persistent identity: range2203922
display id: range2203922
version: 1
direction: direction/forward
top level: BBa_K772009
start: 1615
end: 1745
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2203917
display id: annotation2203917
version: 1
top level: BBa_K772009
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K772009/annotation2203917/range2203917/1

https://synbiohub.org/public/igem/BBa_K772009/annotation2203917/range2203917/1
type: Range
persistent identity: range2203917
display id: range2203917
version: 1
direction: direction/forward
top level: BBa_K772009
start: 1
end: 20
orientation: inline

LasR
type: SequenceAnnotation
persistent identity: annotation2203921
display id: annotation2203921
version: 1
top level: BBa_K772009
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K772009/annotation2203921/range2203921/1

https://synbiohub.org/public/igem/BBa_K772009/annotation2203921/range2203921/1
type: Range
persistent identity: range2203921
display id: range2203921
version: 1
direction: direction/forward
top level: BBa_K772009
start: 858
end: 1614
orientation: inline

Flag tag
type: SequenceAnnotation
persistent identity: annotation2203918
display id: annotation2203918
version: 1
top level: BBa_K772009
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K772009/annotation2203918/range2203918/1

https://synbiohub.org/public/igem/BBa_K772009/annotation2203918/range2203918/1
type: Range
persistent identity: range2203918
display id: range2203918
version: 1
direction: direction/forward
top level: BBa_K772009
start: 21
end: 46
orientation: inline

GFP
type: SequenceAnnotation
persistent identity: annotation2203919
display id: annotation2203919
version: 1
top level: BBa_K772009
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K772009/annotation2203919/range2203919/1

https://synbiohub.org/public/igem/BBa_K772009/annotation2203919/range2203919/1
type: Range
persistent identity: range2203919
display id: range2203919
version: 1
direction: direction/forward
top level: BBa_K772009
start: 47
end: 767
orientation: inline

Anchor-inducer + flag
type: SequenceAnnotation
persistent identity: annotation2203923
display id: annotation2203923
version: 1
top level: BBa_K772009
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K772009/annotation2203923/range2203923/1

https://synbiohub.org/public/igem/BBa_K772009/annotation2203923/range2203923/1
type: Range
persistent identity: range2203923
display id: range2203923
version: 1
direction: direction/forward
top level: BBa_K772009
start: 21
end: 1614
orientation: inline

BBa_K1022100
type: ComponentDefinition
persistent identity: BBa K1022100
display id: BBa_K1022100
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pBAD:AIP receiver:GFP:TT
creator: Maithili Krishnan
created: 2013-08-19T11:00:00Z
modified: 2015-05-08T01:08:44Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1329_
m user id: 0
owner id: 15691
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This plasmid contains code for pBAD promoter, AIP receiver Agr A and Agr C, GFP along with double terminator.
mutable notes: Sequence was cloned using BBa_E0240 and BBa_I746103 from iGEM parts registry.
mutable provenance: E.coli
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_K206000
component: BBa_E0240
component: BBa_I746103
sequence annotation: BBa_E0240
sequence annotation: BBa_I746103
sequence annotation: BBa_K206000
sequence: https://synbiohub.org/public/igem/BBa_K1022100_sequence/1

BBa_K206000
type: Component
persistent identity: component2332885
display id: component2332885
version: 1
top level: BBa_K1022100
access: public
definition: https://synbiohub.org/public/igem/BBa_K206000/1

BBa_E0240
type: Component
persistent identity: component2332910
display id: component2332910
version: 1
top level: BBa_K1022100
access: public
definition: https://synbiohub.org/public/igem/BBa_E0240/1

BBa_I746103
type: Component
persistent identity: component2332899
display id: component2332899
version: 1
top level: BBa_K1022100
access: public
definition: https://synbiohub.org/public/igem/BBa_I746103/1

BBa_E0240
type: SequenceAnnotation
persistent identity: annotation2332910
display id: annotation2332910
version: 1
top level: BBa_K1022100
location: https://synbiohub.org/public/igem/BBa_K1022100/annotation2332910/range2332910/1
component: BBa_E0240

https://synbiohub.org/public/igem/BBa_K1022100/annotation2332910/range2332910/1
type: Range
persistent identity: range2332910
display id: range2332910
version: 1
direction: direction/reverse
top level: BBa_K1022100
start: 2308
end: 3183
orientation: inline

BBa_I746103
type: SequenceAnnotation
persistent identity: annotation2332899
display id: annotation2332899
version: 1
top level: BBa_K1022100
location: https://synbiohub.org/public/igem/BBa_K1022100/annotation2332899/range2332899/1
component: BBa_I746103

https://synbiohub.org/public/igem/BBa_K1022100/annotation2332899/range2332899/1
type: Range
persistent identity: range2332899
display id: range2332899
version: 1
direction: direction/reverse
top level: BBa_K1022100
start: 139
end: 2299
orientation: inline

BBa_K206000
type: SequenceAnnotation
persistent identity: annotation2332885
display id: annotation2332885
version: 1
top level: BBa_K1022100
location: https://synbiohub.org/public/igem/BBa_K1022100/annotation2332885/range2332885/1
component: BBa_K206000

https://synbiohub.org/public/igem/BBa_K1022100/annotation2332885/range2332885/1
type: Range
persistent identity: range2332885
display id: range2332885
version: 1
direction: direction/reverse
top level: BBa_K1022100
start: 1
end: 130
orientation: inline

BBa_J70595
type: ComponentDefinition
persistent identity: BBa J70595
display id: BBa_J70595
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RFC12 Green Fluorescent Protein
creator: Joseph Lynch
created: 2010-06-22T11:00:00Z
modified: 2015-05-08T01:08:25Z
discontinued: false
dominant: true
experience: experience/None
group u list: _41_
m user id: 0
owner id: 6384
owning group id: 41
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This is a RFC12 compatible part based off of BBa_E0040.
mutable notes: Amplified via colony PCR directly from the BBa_E0040 cell stock. Forward Primer: 5' GTTTCTT C GAATTC GCGGCGC T ACTAGT CGTAAAGGAGAAGAACTTTTCACTG 3' Reverse Primer: 3' CACATGGCATGGATGAACTATACAAA 5' so 5' GTTTCTT C CTGCAG CGGCCGC T GCTAGC TTTGTATAGTTCATCCATGCCATGTG 3'
mutable provenance: BBa_E0040
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: GFP
sequence: https://synbiohub.org/public/igem/BBa_J70595_sequence/1

GFP
type: SequenceAnnotation
persistent identity: annotation2071481
display id: annotation2071481
version: 1
top level: BBa_J70595
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_J70595/annotation2071481/range2071481/1

https://synbiohub.org/public/igem/BBa_J70595/annotation2071481/range2071481/1
type: Range
persistent identity: range2071481
display id: range2071481
version: 1
direction: direction/forward
top level: BBa_J70595
start: 1
end: 710
orientation: inline

BBa_K1031222
type: ComponentDefinition
persistent identity: BBa K1031222
display id: BBa_K1031222
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Po-B0032-sfGFP-Terminator (DmpR)
creator: He Shuaixin
created: 2013-09-08T11:00:00Z
modified: 2015-05-08T01:08:47Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1338_
m user id: 0
owner id: 15843
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: Po-32-sfGFP-TT (DmpR)
mutable notes: molecular cloning
mutable provenance: synthesis, lab made
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: sfGFP
sequence annotation: B0032
sequence annotation: po promoter
sequence annotation: B0015
sequence: https://synbiohub.org/public/igem/BBa_K1031222_sequence/1

sfGFP
type: SequenceAnnotation
persistent identity: annotation2354356
display id: annotation2354356
version: 1
top level: BBa_K1031222
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1031222/annotation2354356/range2354356/1

https://synbiohub.org/public/igem/BBa_K1031222/annotation2354356/range2354356/1
type: Range
persistent identity: range2354356
display id: range2354356
version: 1
direction: direction/forward
top level: BBa_K1031222
start: 199
end: 921
orientation: inline

B0032
type: SequenceAnnotation
persistent identity: annotation2354355
display id: annotation2354355
version: 1
top level: BBa_K1031222
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1031222/annotation2354355/range2354355/1

https://synbiohub.org/public/igem/BBa_K1031222/annotation2354355/range2354355/1
type: Range
persistent identity: range2354355
display id: range2354355
version: 1
direction: direction/forward
top level: BBa_K1031222
start: 180
end: 182
orientation: inline

po promoter
type: SequenceAnnotation
persistent identity: annotation2354354
display id: annotation2354354
version: 1
top level: BBa_K1031222
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1031222/annotation2354354/range2354354/1

https://synbiohub.org/public/igem/BBa_K1031222/annotation2354354/range2354354/1
type: Range
persistent identity: range2354354
display id: range2354354
version: 1
direction: direction/forward
top level: BBa_K1031222
start: 1
end: 171
orientation: inline

B0015
type: SequenceAnnotation
persistent identity: annotation2354357
display id: annotation2354357
version: 1
top level: BBa_K1031222
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1031222/annotation2354357/range2354357/1

https://synbiohub.org/public/igem/BBa_K1031222/annotation2354357/range2354357/1
type: Range
persistent identity: range2354357
display id: range2354357
version: 1
direction: direction/forward
top level: BBa_K1031222
start: 930
end: 1058
orientation: inline

SP-Lipocal
type: ComponentDefinition
persistent identity: BBa K157034
display id: BBa_K157034
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: SP-Lipocalin-GGGSlinker-Tm-Fluolinker-cYFP
creator: iGEM Team Freiburg 2008
created: 2008-10-25T11:00:00Z
modified: 2015-05-08T01:10:55Z
discontinued: false
dominant: false
experience: experience/None
group u list: _232_
m user id: 0
owner id: 1673
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: A composite part consisting of the signal peptide of Erbb1/EGF-receptor, scFV-antiNIP, GGGSflexlinker (extracellular part), the trans-membrane region of the Erbb1/EGF-receptor and the C-terminal part of the split fluorophor YFP with a fluolinker joined to its N-Terminus (intracellular part).
mutable notes: none
mutable provenance: Derived by cloning of the parts BBa_K157001, BBa_K157004, BBa_K157013, BBa_K157002, BBa_K157009 and BBa_K157007. For further information see the description of the parts mentioned above.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: Lipocalin
sequence annotation: Scar (Thr, Gly)
sequence annotation: C-Term_YFP
sequence annotation: AgeI
sequence annotation: SP
sequence annotation: Tm
sequence annotation: GGGS-Linker
sequence annotation: Fluolinker
sequence annotation: NgoMI
sequence annotation: SP-Lipocalin-GGGSlinker-Tm-Fluolinker-cYFP
sequence: https://synbiohub.org/public/igem/BBa_K157034_sequence/1

Lipocalin
type: SequenceAnnotation
persistent identity: annotation1993352
display id: annotation1993352
version: 1
top level: SP-Lipocal
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157034/annotation1993352/range1993352/1

https://synbiohub.org/public/igem/BBa_K157034/annotation1993352/range1993352/1
type: Range
persistent identity: range1993352
display id: range1993352
version: 1
direction: direction/forward
top level: SP-Lipocal
start: 88
end: 609
orientation: inline

Scar (Thr, Gly)
type: SequenceAnnotation
persistent identity: annotation1993359
display id: annotation1993359
version: 1
top level: SP-Lipocal
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157034/annotation1993359/range1993359/1

https://synbiohub.org/public/igem/BBa_K157034/annotation1993359/range1993359/1
type: Range
persistent identity: range1993359
display id: range1993359
version: 1
direction: direction/forward
top level: SP-Lipocal
start: 793
end: 798
orientation: inline

Scar (Thr, Gly)
type: SequenceAnnotation
persistent identity: annotation1993353
display id: annotation1993353
version: 1
top level: SP-Lipocal
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157034/annotation1993353/range1993353/1

https://synbiohub.org/public/igem/BBa_K157034/annotation1993353/range1993353/1
type: Range
persistent identity: range1993353
display id: range1993353
version: 1
direction: direction/forward
top level: SP-Lipocal
start: 610
end: 615
orientation: inline

C-Term_YFP
type: SequenceAnnotation
persistent identity: annotation1993360
display id: annotation1993360
version: 1
top level: SP-Lipocal
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157034/annotation1993360/range1993360/1

https://synbiohub.org/public/igem/BBa_K157034/annotation1993360/range1993360/1
type: Range
persistent identity: range1993360
display id: range1993360
version: 1
direction: direction/forward
top level: SP-Lipocal
start: 799
end: 1041
orientation: inline

AgeI
type: SequenceAnnotation
persistent identity: annotation1990277
display id: annotation1990277
version: 1
top level: SP-Lipocal
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157034/annotation1990277/range1990277/1

https://synbiohub.org/public/igem/BBa_K157034/annotation1990277/range1990277/1
type: Range
persistent identity: range1990277
display id: range1990277
version: 1
direction: direction/forward
top level: SP-Lipocal
start: 1042
end: 1047
orientation: inline

Scar (Thr, Gly)
type: SequenceAnnotation
persistent identity: annotation1993357
display id: annotation1993357
version: 1
top level: SP-Lipocal
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157034/annotation1993357/range1993357/1

https://synbiohub.org/public/igem/BBa_K157034/annotation1993357/range1993357/1
type: Range
persistent identity: range1993357
display id: range1993357
version: 1
direction: direction/forward
top level: SP-Lipocal
start: 736
end: 741
orientation: inline

SP
type: SequenceAnnotation
persistent identity: annotation1993350
display id: annotation1993350
version: 1
top level: SP-Lipocal
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157034/annotation1993350/range1993350/1

https://synbiohub.org/public/igem/BBa_K157034/annotation1993350/range1993350/1
type: Range
persistent identity: range1993350
display id: range1993350
version: 1
direction: direction/forward
top level: SP-Lipocal
start: 10
end: 81
orientation: inline

Scar (Thr, Gly)
type: SequenceAnnotation
persistent identity: annotation1993351
display id: annotation1993351
version: 1
top level: SP-Lipocal
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157034/annotation1993351/range1993351/1

https://synbiohub.org/public/igem/BBa_K157034/annotation1993351/range1993351/1
type: Range
persistent identity: range1993351
display id: range1993351
version: 1
direction: direction/forward
top level: SP-Lipocal
start: 82
end: 87
orientation: inline

Tm
type: SequenceAnnotation
persistent identity: annotation1993356
display id: annotation1993356
version: 1
top level: SP-Lipocal
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157034/annotation1993356/range1993356/1

https://synbiohub.org/public/igem/BBa_K157034/annotation1993356/range1993356/1
type: Range
persistent identity: range1993356
display id: range1993356
version: 1
direction: direction/forward
top level: SP-Lipocal
start: 667
end: 735
orientation: inline

GGGS-Linker
type: SequenceAnnotation
persistent identity: annotation1993354
display id: annotation1993354
version: 1
top level: SP-Lipocal
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157034/annotation1993354/range1993354/1

https://synbiohub.org/public/igem/BBa_K157034/annotation1993354/range1993354/1
type: Range
persistent identity: range1993354
display id: range1993354
version: 1
direction: direction/forward
top level: SP-Lipocal
start: 616
end: 660
orientation: inline

Scar (Thr, Gly)
type: SequenceAnnotation
persistent identity: annotation1993355
display id: annotation1993355
version: 1
top level: SP-Lipocal
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157034/annotation1993355/range1993355/1

https://synbiohub.org/public/igem/BBa_K157034/annotation1993355/range1993355/1
type: Range
persistent identity: range1993355
display id: range1993355
version: 1
direction: direction/forward
top level: SP-Lipocal
start: 661
end: 666
orientation: inline

Fluolinker
type: SequenceAnnotation
persistent identity: annotation1993358
display id: annotation1993358
version: 1
top level: SP-Lipocal
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157034/annotation1993358/range1993358/1

https://synbiohub.org/public/igem/BBa_K157034/annotation1993358/range1993358/1
type: Range
persistent identity: range1993358
display id: range1993358
version: 1
direction: direction/forward
top level: SP-Lipocal
start: 742
end: 792
orientation: inline

NgoMI
type: SequenceAnnotation
persistent identity: annotation1990276
display id: annotation1990276
version: 1
top level: SP-Lipocal
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157034/annotation1990276/range1990276/1

https://synbiohub.org/public/igem/BBa_K157034/annotation1990276/range1990276/1
type: Range
persistent identity: range1990276
display id: range1990276
version: 1
direction: direction/forward
top level: SP-Lipocal
start: 4
end: 9
orientation: inline

SP-Lipocalin-GGGSlinker-Tm-Fluolinker-cYFP
type: SequenceAnnotation
persistent identity: annotation1990278
display id: annotation1990278
version: 1
top level: SP-Lipocal
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K157034/annotation1990278/range1990278/1

https://synbiohub.org/public/igem/BBa_K157034/annotation1990278/range1990278/1
type: Range
persistent identity: range1990278
display id: range1990278
version: 1
direction: direction/forward
top level: SP-Lipocal
start: 1
end: 1047
orientation: inline

BBa_J97001
type: ComponentDefinition
persistent identity: BBa J97001
display id: BBa_J97001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: P-Free JuniperGFP (Green Fluorescent Protein)
creator: Drew Endy
created: 2014-02-18T12:00:00Z
modified: 2015-05-08T01:08:32Z
discontinued: false
dominant: false
experience: experience/None
group u list: _41_1_
m user id: 0
owner id: 747
owning group id: 63
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This part was created by DNA 2.0 as part of their IP-Free series of fluorescent and chromogenic proteins. It is available to use under the BioBrick Public Agreement. Excitation 508nm, Emission 521nm Molecular Weight: 26.6kDA Length: 237aa
mutable notes: This sequence is fully synthetic, (non-Aequorea) and is codon optimized for expression in E. coli.
mutable provenance: This synthetic part was created through a gene engineering and synthesis project at DNA 2.0.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence: https://synbiohub.org/public/igem/BBa_J97001_sequence/1

pBAD-GFP
type: ComponentDefinition
persistent identity: BBa K845000
display id: BBa_K845000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: iGEM 2012 Grenoble Team proposes a new design and application to the pBAD promoter(paraBAD).
creator: Gr??gory Hansen
created: 2012-09-25T11:00:00Z
modified: 2015-05-08T01:13:33Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1105_
m user id: 0
owner id: 12465
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: In order to create a biological AND gate the 2012 Grenoble team worked on the cAMP-CRP complex and arabinose inductible promoter. The activity of the promoter was tested under several conditions (http://2012.igem.org/Team:Grenoble/Biology/Protocols/AND_test)using a GFP as a reporter (on a pUA66). We tested this activity in BW25113 ΔcyaA in different culture media (Glucose + Acetate; Glycerol; Acetate).
mutable notes: Design note: http://www.weizmann.ac.il/mcb/UriAlon/Papers/Zaslaver_Ecoli_library.pdf
mutable provenance: The part comes from the plasmid collection Alon (http://www.weizmann.ac.il/mcb/UriAlon/Papers/Zaslaver_Ecoli_library.pdf). However, pBAD is a natural promoter in E. Coli. It regulates the production of three proteins (AraA, AraB and AraD) which form the arabinose operon. Their production enables the use of arabinose as a carbon source. It has six regulation sites, five of them are devoted to AraC fixation (three are repressing, one has a dual activity and one is an activator); the last one is a CRP binding site (positive activity). This promoter is activated when both activated CRP and AraC are bind to it. GFP comes from the protein comes from Aequorea victoria. This jellyfish uses GFP (Green Fluorescent Protein) in order to convert the blue luminescence emitted by the aequorine into a green luminescence. Aequorea victoria: is a jellyfish that can be found off the coast of north America.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K845001
component: BBa_K845002
sequence annotation: BBa_K845001
sequence annotation: BBa_K845002
sequence: https://synbiohub.org/public/igem/BBa_K845000_sequence/1

BBa_K845001
type: Component
persistent identity: component2203944
display id: component2203944
version: 1
top level: pBAD-GFP
access: public
definition: https://synbiohub.org/public/igem/BBa_K845001/1

BBa_K845002
type: Component
persistent identity: component2203947
display id: component2203947
version: 1
top level: pBAD-GFP
access: public
definition: GFP

BBa_K845001
type: SequenceAnnotation
persistent identity: annotation2203944
display id: annotation2203944
version: 1
top level: pBAD-GFP
location: https://synbiohub.org/public/igem/BBa_K845000/annotation2203944/range2203944/1
component: BBa_K845001

https://synbiohub.org/public/igem/BBa_K845000/annotation2203944/range2203944/1
type: Range
persistent identity: range2203944
display id: range2203944
version: 1
direction: direction/reverse
top level: pBAD-GFP
start: 1
end: 499
orientation: inline

BBa_K845002
type: SequenceAnnotation
persistent identity: annotation2203947
display id: annotation2203947
version: 1
top level: pBAD-GFP
location: https://synbiohub.org/public/igem/BBa_K845000/annotation2203947/range2203947/1
component: BBa_K845002

https://synbiohub.org/public/igem/BBa_K845000/annotation2203947/range2203947/1
type: Range
persistent identity: range2203947
display id: range2203947
version: 1
direction: direction/reverse
top level: pBAD-GFP
start: 500
end: 1242
orientation: inline

BBa_K1486026
type: ComponentDefinition
persistent identity: BBa K1486026
display id: BBa_K1486026
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: sfGFP + Kanamycin resistance for yeast
creator: EPFL iGem team 2014
created: 2014-09-25T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: To edit
mutable notes: To edit
mutable provenance: To edit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000324
role: partType/Tag
sequence annotation: Adh1 terminator
sequence annotation: BBa_K1486023
sequence annotation: Pbs2 flanking
sequence annotation: BBa_K1486024
sequence annotation: 8 a.a linker
sequence: https://synbiohub.org/public/igem/BBa_K1486026_sequence/1

Adh1 terminator
type: SequenceAnnotation
persistent identity: annotation2417050
display id: annotation2417050
version: 1
top level: BBa_K1486026
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1486026/annotation2417050/range2417050/1

https://synbiohub.org/public/igem/BBa_K1486026/annotation2417050/range2417050/1
type: Range
persistent identity: range2417050
display id: range2417050
version: 1
direction: direction/forward
top level: BBa_K1486026
start: 811
end: 999
orientation: inline

BBa_K1486023
type: SequenceAnnotation
persistent identity: annotation2417049
display id: annotation2417049
version: 1
top level: BBa_K1486026
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1486026/annotation2417049/range2417049/1

https://synbiohub.org/public/igem/BBa_K1486026/annotation2417049/range2417049/1
type: Range
persistent identity: range2417049
display id: range2417049
version: 1
direction: direction/forward
top level: BBa_K1486026
start: 75
end: 791
orientation: inline

Pbs2 flanking
type: SequenceAnnotation
persistent identity: annotation2417047
display id: annotation2417047
version: 1
top level: BBa_K1486026
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1486026/annotation2417047/range2417047/1

https://synbiohub.org/public/igem/BBa_K1486026/annotation2417047/range2417047/1
type: Range
persistent identity: range2417047
display id: range2417047
version: 1
direction: direction/forward
top level: BBa_K1486026
start: 1
end: 50
orientation: inline

BBa_K1486024
type: SequenceAnnotation
persistent identity: annotation2417051
display id: annotation2417051
version: 1
top level: BBa_K1486026
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1486026/annotation2417051/range2417051/1

https://synbiohub.org/public/igem/BBa_K1486026/annotation2417051/range2417051/1
type: Range
persistent identity: range2417051
display id: range2417051
version: 1
direction: direction/forward
top level: BBa_K1486026
start: 1073
end: 2430
orientation: inline

Pbs2 flanking
type: SequenceAnnotation
persistent identity: annotation2417052
display id: annotation2417052
version: 1
top level: BBa_K1486026
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1486026/annotation2417052/range2417052/1

https://synbiohub.org/public/igem/BBa_K1486026/annotation2417052/range2417052/1
type: Range
persistent identity: range2417052
display id: range2417052
version: 1
direction: direction/forward
top level: BBa_K1486026
start: 2485
end: 2535
orientation: inline

8 a.a linker
type: SequenceAnnotation
persistent identity: annotation2417048
display id: annotation2417048
version: 1
top level: BBa_K1486026
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1486026/annotation2417048/range2417048/1

https://synbiohub.org/public/igem/BBa_K1486026/annotation2417048/range2417048/1
type: Range
persistent identity: range2417048
display id: range2417048
version: 1
direction: direction/forward
top level: BBa_K1486026
start: 51
end: 74
orientation: inline

BBa_K1819005
type: ComponentDefinition
persistent identity: BBa K1819005
display id: BBa_K1819005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Constitutive promoter (J23101) and GFP (K1819000)
creator: La??s Ribovski
created: 2015-09-15T11:00:00Z
modified: 2015-09-16T05:14:28Z
discontinued: false
dominant: false
group u list: _2244_
m user id: 21190
owner id: 21190
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Constitutive promotor from Anderson library (BBa_J23101) assembled to green fluorescent protein attached to linker (BBa_K1819000).
mutable notes: .
mutable provenance: This part results from the assembly of Bba_J23101 and BBa_K1819000
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_S05308
component: BBa_J23101
sequence annotation: BBa_S05308
sequence annotation: BBa_J23101
sequence: https://synbiohub.org/public/igem/BBa_K1819005_sequence/1

BBa_S05308
type: Component
persistent identity: component2462431
display id: component2462431
version: 1
top level: BBa_K1819005
access: public
definition: https://synbiohub.org/public/igem/BBa_S05308/1

BBa_J23101
type: Component
persistent identity: component2462420
display id: component2462420
version: 1
top level: BBa_K1819005
access: public
definition: https://synbiohub.org/public/igem/BBa_J23101/1

BBa_S05308
type: SequenceAnnotation
persistent identity: annotation2462431
display id: annotation2462431
version: 1
top level: BBa_K1819005
location: https://synbiohub.org/public/igem/BBa_K1819005/annotation2462431/range2462431/1
component: BBa_S05308

https://synbiohub.org/public/igem/BBa_K1819005/annotation2462431/range2462431/1
type: Range
persistent identity: range2462431
display id: range2462431
version: 1
direction: direction/reverse
top level: BBa_K1819005
start: 44
end: 930
orientation: inline

BBa_J23101
type: SequenceAnnotation
persistent identity: annotation2462420
display id: annotation2462420
version: 1
top level: BBa_K1819005
location: https://synbiohub.org/public/igem/BBa_K1819005/annotation2462420/range2462420/1
component: BBa_J23101

https://synbiohub.org/public/igem/BBa_K1819005/annotation2462420/range2462420/1
type: Range
persistent identity: range2462420
display id: range2462420
version: 1
direction: direction/reverse
top level: BBa_K1819005
start: 1
end: 35
orientation: inline

BBa_K2040122
type: ComponentDefinition
persistent identity: BBa K2040122
display id: BBa_K2040122
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: KillerRed + NLS
creator: Chilam Poon
created: 2016-10-13T11:00:00Z
modified: 2016-10-19T01:35:29Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2507_
m user id: 30360
owner id: 30360
owning group id: 9
status: status/Planning
bookmark: false
mutable description: A SV40 nuclear localization signal was added to the phototoxic protein KillerRed.
mutable notes: NLS
mutable provenance: BBa_K1184000
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: KillerRed
sequence annotation: SV40 NLS
sequence: https://synbiohub.org/public/igem/BBa_K2040122_sequence/1

KillerRed
type: SequenceAnnotation
persistent identity: annotation2518117
display id: annotation2518117
version: 1
top level: BBa_K2040122
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K2040122/annotation2518117/range2518117/1

https://synbiohub.org/public/igem/BBa_K2040122/annotation2518117/range2518117/1
type: Range
persistent identity: range2518117
display id: range2518117
version: 1
direction: direction/forward
top level: BBa_K2040122
start: 1
end: 717
orientation: inline

SV40 NLS
type: SequenceAnnotation
persistent identity: annotation2502105
display id: annotation2502105
version: 1
top level: BBa_K2040122
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K2040122/annotation2502105/range2502105/1

https://synbiohub.org/public/igem/BBa_K2040122/annotation2502105/range2502105/1
type: Range
persistent identity: range2502105
display id: range2502105
version: 1
direction: direction/forward
top level: BBa_K2040122
start: 718
end: 741
orientation: inline

BBa_K1031520
type: ComponentDefinition
persistent identity: BBa K1031520
display id: BBa_K1031520
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Pg-B0031-sfGFP-Terminator (HpaR)
creator: He Shuaixin
created: 2013-09-08T11:00:00Z
modified: 2015-05-08T01:08:47Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1338_
m user id: 0
owner id: 15843
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Pg-sfGFP-TT (HpaR)
mutable notes: molecular cloning
mutable provenance: synthesis, lab made
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: B0031
sequence annotation: Pg (HpaR)
sequence annotation: sfGFP
sequence annotation: B0015
sequence: https://synbiohub.org/public/igem/BBa_K1031520_sequence/1

B0031
type: SequenceAnnotation
persistent identity: annotation2362410
display id: annotation2362410
version: 1
top level: BBa_K1031520
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1031520/annotation2362410/range2362410/1

https://synbiohub.org/public/igem/BBa_K1031520/annotation2362410/range2362410/1
type: Range
persistent identity: range2362410
display id: range2362410
version: 1
direction: direction/forward
top level: BBa_K1031520
start: 234
end: 247
orientation: inline

Pg (HpaR)
type: SequenceAnnotation
persistent identity: annotation2362409
display id: annotation2362409
version: 1
top level: BBa_K1031520
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1031520/annotation2362409/range2362409/1

https://synbiohub.org/public/igem/BBa_K1031520/annotation2362409/range2362409/1
type: Range
persistent identity: range2362409
display id: range2362409
version: 1
direction: direction/forward
top level: BBa_K1031520
start: 1
end: 227
orientation: inline

sfGFP
type: SequenceAnnotation
persistent identity: annotation2362411
display id: annotation2362411
version: 1
top level: BBa_K1031520
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1031520/annotation2362411/range2362411/1

https://synbiohub.org/public/igem/BBa_K1031520/annotation2362411/range2362411/1
type: Range
persistent identity: range2362411
display id: range2362411
version: 1
direction: direction/forward
top level: BBa_K1031520
start: 254
end: 976
orientation: inline

B0015
type: SequenceAnnotation
persistent identity: annotation2362412
display id: annotation2362412
version: 1
top level: BBa_K1031520
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1031520/annotation2362412/range2362412/1

https://synbiohub.org/public/igem/BBa_K1031520/annotation2362412/range2362412/1
type: Range
persistent identity: range2362412
display id: range2362412
version: 1
direction: direction/forward
top level: BBa_K1031520
start: 983
end: 1111
orientation: inline

BBa_K1486050
type: ComponentDefinition
persistent identity: BBa K1486050
display id: BBa_K1486050
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CpxR promoter RV + RFP
creator: EPFL iGem team 2014
created: 2014-10-06T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: to edit
mutable notes: to edit
mutable provenance: to edit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_I13507
component: BBa_K1486047
sequence annotation: BBa_K1486047
sequence annotation: BBa_I13507
sequence: https://synbiohub.org/public/igem/BBa_K1486050_sequence/1

BBa_I13507
type: Component
persistent identity: component2398462
display id: component2398462
version: 1
top level: BBa_K1486050
access: public
definition: https://synbiohub.org/public/igem/BBa_I13507/1

BBa_K1486047
type: Component
persistent identity: component2398450
display id: component2398450
version: 1
top level: BBa_K1486050
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486047/1

BBa_K1486047
type: SequenceAnnotation
persistent identity: annotation2398450
display id: annotation2398450
version: 1
top level: BBa_K1486050
location: https://synbiohub.org/public/igem/BBa_K1486050/annotation2398450/range2398450/1
component: BBa_K1486047

https://synbiohub.org/public/igem/BBa_K1486050/annotation2398450/range2398450/1
type: Range
persistent identity: range2398450
display id: range2398450
version: 1
direction: direction/reverse
top level: BBa_K1486050
start: 1
end: 146
orientation: inline

BBa_I13507
type: SequenceAnnotation
persistent identity: annotation2398462
display id: annotation2398462
version: 1
top level: BBa_K1486050
location: https://synbiohub.org/public/igem/BBa_K1486050/annotation2398462/range2398462/1
component: BBa_I13507

https://synbiohub.org/public/igem/BBa_K1486050/annotation2398462/range2398462/1
type: Range
persistent identity: range2398462
display id: range2398462
version: 1
direction: direction/reverse
top level: BBa_K1486050
start: 147
end: 1007
orientation: inline

BBa_J97003
type: ComponentDefinition
persistent identity: BBa J97003
display id: BBa_J97003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: IP-Free TannenRFP (Red Fluorescent Protein)
creator: Drew Endy
created: 2014-02-18T12:00:00Z
modified: 2015-05-08T01:08:32Z
discontinued: false
dominant: false
experience: experience/None
group u list: _41_1_
m user id: 0
owner id: 747
owning group id: 63
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: x
mutable notes: This sequence is fully synthetic, (non-Aequorea) and is codon optimized for expression in E. coli.
mutable provenance: This synthetic part was created through a gene engineering and synthesis project at DNA 2.0.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence: https://synbiohub.org/public/igem/BBa_J97003_sequence/1

eGFP-TS
type: ComponentDefinition
persistent identity: BBa K678008
display id: BBa_K678008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: eGFP-TS
creator: DTU-Danmark-2
created: 2011-09-12T11:00:00Z
modified: 2015-05-08T01:13:04Z
discontinued: false
dominant: false
experience: experience/None
group u list: _882_
m user id: 0
owner id: 10556
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part was designed for the Plug'n'Play with DNA assembly standard (RFC 80), therefore this part contains tails at the 5' and 3' ends as shown on the figure below.
mutable notes: No
mutable provenance: The Danish Cancer Society
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: eGFP-TS
sequence annotation: Kozak
sequence annotation: Linker 3
sequence: https://synbiohub.org/public/igem/BBa_K678008_sequence/1

eGFP-TS
type: SequenceAnnotation
persistent identity: annotation2127770
display id: annotation2127770
version: 1
top level: eGFP-TS
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K678008/annotation2127770/range2127770/1

https://synbiohub.org/public/igem/BBa_K678008/annotation2127770/range2127770/1
type: Range
persistent identity: range2127770
display id: range2127770
version: 1
direction: direction/forward
top level: eGFP-TS
start: 16
end: 736
orientation: inline

Kozak
type: SequenceAnnotation
persistent identity: annotation2127769
display id: annotation2127769
version: 1
top level: eGFP-TS
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678008/annotation2127769/range2127769/1

https://synbiohub.org/public/igem/BBa_K678008/annotation2127769/range2127769/1
type: Range
persistent identity: range2127769
display id: range2127769
version: 1
direction: direction/forward
top level: eGFP-TS
start: 9
end: 19
orientation: inline

Linker 3
type: SequenceAnnotation
persistent identity: annotation2127768
display id: annotation2127768
version: 1
top level: eGFP-TS
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678008/annotation2127768/range2127768/1

https://synbiohub.org/public/igem/BBa_K678008/annotation2127768/range2127768/1
type: Range
persistent identity: range2127768
display id: range2127768
version: 1
direction: direction/forward
top level: eGFP-TS
start: 1
end: 9
orientation: inline

BBa_K1351021
type: ComponentDefinition
persistent identity: BBa K1351021
display id: BBa_K1351021
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Monomeric Red Fluorescent Protein from Discosoma striata
creator: Anna Sommer
created: 2014-09-29T11:00:00Z
modified: 2016-01-26T01:58:37Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1726_
m user id: 4206
owner id: 20043
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: Mutant of the Biobrick BBa_E1010, in order to make it compatible with the Freiburg-Standard, two AgeI-Restriction sites were deleted monomeric RFP: Red Fluorescent Protein. Excitation peak: 584 nm Emission peak: 607 nm
mutable notes: The Biobrick has the Following Prefix and Suffix: Gatcgaattccgcggccgcttctagataaggaggagccggc Gatcctgcagcggccgctactagtattaaccggt
mutable provenance: Discosoma striata (coral)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1351021_sequence/1

mCherry-TS
type: ComponentDefinition
persistent identity: BBa K678014
display id: BBa_K678014
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: mCherry-TS
creator: DTU-Danmark-2
created: 2011-09-12T11:00:00Z
modified: 2015-05-08T01:13:04Z
discontinued: false
dominant: false
experience: experience/None
group u list: _882_
m user id: 0
owner id: 10556
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part was designed for the Plug'n'Play with DNA assembly standard (RFC 80), therefore this part contains tails at the 5' and 3' ends as shown on the figure below.
mutable notes: No
mutable provenance: The Danish Canser Society
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: Linker 3
sequence annotation: misc
sequence annotation: mCherry-TS
sequence: https://synbiohub.org/public/igem/BBa_K678014_sequence/1

Linker 3
type: SequenceAnnotation
persistent identity: annotation2127756
display id: annotation2127756
version: 1
top level: mCherry-TS
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678014/annotation2127756/range2127756/1

https://synbiohub.org/public/igem/BBa_K678014/annotation2127756/range2127756/1
type: Range
persistent identity: range2127756
display id: range2127756
version: 1
direction: direction/forward
top level: mCherry-TS
start: 1
end: 9
orientation: inline

misc
type: SequenceAnnotation
persistent identity: annotation2127780
display id: annotation2127780
version: 1
top level: mCherry-TS
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678014/annotation2127780/range2127780/1

https://synbiohub.org/public/igem/BBa_K678014/annotation2127780/range2127780/1
type: Range
persistent identity: range2127780
display id: range2127780
version: 1
direction: direction/forward
top level: mCherry-TS
start: 10
end: 20
orientation: inline

mCherry-TS
type: SequenceAnnotation
persistent identity: annotation2127757
display id: annotation2127757
version: 1
top level: mCherry-TS
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K678014/annotation2127757/range2127757/1

https://synbiohub.org/public/igem/BBa_K678014/annotation2127757/range2127757/1
type: Range
persistent identity: range2127757
display id: range2127757
version: 1
direction: direction/forward
top level: mCherry-TS
start: 17
end: 727
orientation: inline

BBa_K1486034
type: ComponentDefinition
persistent identity: BBa K1486034
display id: BBa_K1486034
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Yeast optimized superfolder GFP C-terminus (215-238)
creator: EPFL iGem team 2014
created: 2014-09-26T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: To edit
mutable notes: To edit
mutable provenance: To edit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000324
role: partType/Tag
sequence annotation: Yeast optimized sfGFP C-terminus
sequence: https://synbiohub.org/public/igem/BBa_K1486034_sequence/1

Yeast optimized sfGFP C-terminus
type: SequenceAnnotation
persistent identity: annotation2417784
display id: annotation2417784
version: 1
top level: BBa_K1486034
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1486034/annotation2417784/range2417784/1

https://synbiohub.org/public/igem/BBa_K1486034/annotation2417784/range2417784/1
type: Range
persistent identity: range2417784
display id: range2417784
version: 1
direction: direction/forward
top level: BBa_K1486034
start: 1
end: 75
orientation: inline

BBa_K1486032
type: ComponentDefinition
persistent identity: BBa K1486032
display id: BBa_K1486032
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: sfGFP + ADH1 terminator + Ura3 cassette
creator: EPFL iGem team 2014
created: 2014-09-26T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: To edit
mutable notes: To edit
mutable provenance: To edit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: 8 a.a linker
sequence annotation: BBa_K1486031
sequence annotation: BBa_K1486023
sequence annotation: ADH1 terminator
sequence annotation: Hog1 3' flanking region
sequence annotation: Hog1 5' flanking region
sequence: https://synbiohub.org/public/igem/BBa_K1486032_sequence/1

8 a.a linker
type: SequenceAnnotation
persistent identity: annotation2417800
display id: annotation2417800
version: 1
top level: BBa_K1486032
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1486032/annotation2417800/range2417800/1

https://synbiohub.org/public/igem/BBa_K1486032/annotation2417800/range2417800/1
type: Range
persistent identity: range2417800
display id: range2417800
version: 1
direction: direction/forward
top level: BBa_K1486032
start: 48
end: 71
orientation: inline

BBa_K1486031
type: SequenceAnnotation
persistent identity: annotation2417804
display id: annotation2417804
version: 1
top level: BBa_K1486032
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1486032/annotation2417804/range2417804/1

https://synbiohub.org/public/igem/BBa_K1486032/annotation2417804/range2417804/1
type: Range
persistent identity: range2417804
display id: range2417804
version: 1
direction: direction/forward
top level: BBa_K1486032
start: 1070
end: 2430
orientation: inline

BBa_K1486023
type: SequenceAnnotation
persistent identity: annotation2417801
display id: annotation2417801
version: 1
top level: BBa_K1486032
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1486032/annotation2417801/range2417801/1

https://synbiohub.org/public/igem/BBa_K1486032/annotation2417801/range2417801/1
type: Range
persistent identity: range2417801
display id: range2417801
version: 1
direction: direction/forward
top level: BBa_K1486032
start: 72
end: 804
orientation: inline

ADH1 terminator
type: SequenceAnnotation
persistent identity: annotation2417803
display id: annotation2417803
version: 1
top level: BBa_K1486032
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1486032/annotation2417803/range2417803/1

https://synbiohub.org/public/igem/BBa_K1486032/annotation2417803/range2417803/1
type: Range
persistent identity: range2417803
display id: range2417803
version: 1
direction: direction/forward
top level: BBa_K1486032
start: 808
end: 996
orientation: inline

Hog1 3' flanking region
type: SequenceAnnotation
persistent identity: annotation2417805
display id: annotation2417805
version: 1
top level: BBa_K1486032
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1486032/annotation2417805/range2417805/1

https://synbiohub.org/public/igem/BBa_K1486032/annotation2417805/range2417805/1
type: Range
persistent identity: range2417805
display id: range2417805
version: 1
direction: direction/forward
top level: BBa_K1486032
start: 2494
end: 2544
orientation: inline

Hog1 5' flanking region
type: SequenceAnnotation
persistent identity: annotation2417799
display id: annotation2417799
version: 1
top level: BBa_K1486032
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1486032/annotation2417799/range2417799/1

https://synbiohub.org/public/igem/BBa_K1486032/annotation2417799/range2417799/1
type: Range
persistent identity: range2417799
display id: range2417799
version: 1
direction: direction/forward
top level: BBa_K1486032
start: 1
end: 47
orientation: inline

GFP_His
type: ComponentDefinition
persistent identity: BBa K863121
display id: BBa_K863121
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (His-tag)
creator: Moritz M??ller
created: 2012-09-19T11:00:00Z
modified: 2016-01-25T01:33:16Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1123_
m user id: 4206
owner id: 13080
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Untagged version of gfp from Repressilator reporter. See the [http://partsregistry.org/wiki/index.php?title=Part:BBa_E0040 untagged version] for source information.
mutable notes: Added Freiburg-restriction-sites for in frame assembly.
mutable provenance: [http://partsregistry.org/Part:BBa_I13522 BBa_I13522]
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: BBa_E0040
sequence annotation: NgoMIV
sequence annotation: ATG
sequence annotation: AgeI
sequence annotation: His-Tag
sequence annotation: TAA
sequence: https://synbiohub.org/public/igem/BBa_K863121_sequence/1

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation2187765
display id: annotation2187765
version: 1
top level: GFP_His
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K863121/annotation2187765/range2187765/1

https://synbiohub.org/public/igem/BBa_K863121/annotation2187765/range2187765/1
type: Range
persistent identity: range2187765
display id: range2187765
version: 1
direction: direction/forward
top level: GFP_His
start: 10
end: 723
orientation: inline

NgoMIV
type: SequenceAnnotation
persistent identity: annotation2187747
display id: annotation2187747
version: 1
top level: GFP_His
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K863121/annotation2187747/range2187747/1

https://synbiohub.org/public/igem/BBa_K863121/annotation2187747/range2187747/1
type: Range
persistent identity: range2187747
display id: range2187747
version: 1
direction: direction/forward
top level: GFP_His
start: 4
end: 9
orientation: inline

ATG
type: SequenceAnnotation
persistent identity: annotation2187748
display id: annotation2187748
version: 1
top level: GFP_His
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K863121/annotation2187748/range2187748/1

https://synbiohub.org/public/igem/BBa_K863121/annotation2187748/range2187748/1
type: Range
persistent identity: range2187748
display id: range2187748
version: 1
direction: direction/forward
top level: GFP_His
start: 1
end: 3
orientation: inline

AgeI
type: SequenceAnnotation
persistent identity: annotation2187756
display id: annotation2187756
version: 1
top level: GFP_His
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K863121/annotation2187756/range2187756/1

https://synbiohub.org/public/igem/BBa_K863121/annotation2187756/range2187756/1
type: Range
persistent identity: range2187756
display id: range2187756
version: 1
direction: direction/forward
top level: GFP_His
start: 742
end: 747
orientation: inline

His-Tag
type: SequenceAnnotation
persistent identity: annotation2187759
display id: annotation2187759
version: 1
top level: GFP_His
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K863121/annotation2187759/range2187759/1

https://synbiohub.org/public/igem/BBa_K863121/annotation2187759/range2187759/1
type: Range
persistent identity: range2187759
display id: range2187759
version: 1
direction: direction/forward
top level: GFP_His
start: 724
end: 741
orientation: inline

TAA
type: SequenceAnnotation
persistent identity: annotation2187751
display id: annotation2187751
version: 1
top level: GFP_His
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K863121/annotation2187751/range2187751/1

https://synbiohub.org/public/igem/BBa_K863121/annotation2187751/range2187751/1
type: Range
persistent identity: range2187751
display id: range2187751
version: 1
direction: direction/forward
top level: GFP_His
start: 748
end: 750
orientation: inline

BBa_K1486006
type: ComponentDefinition
persistent identity: BBa K1486006
display id: BBa_K1486006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: IFP[1]
creator: iGem EPFL 2014
created: 2014-08-24T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1866_
m user id: 0
owner id: 21033
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is the first part of the split infrared fluorescent protein (IFP1.4). To be used with IFP[2].
mutable notes: When fused to another coding sequence, insert a flexible linker
mutable provenance: From Michnick Article. To Edit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: START
sequence annotation: IFP[1]
sequence annotation: STOP
sequence: https://synbiohub.org/public/igem/BBa_K1486006_sequence/1

START
type: SequenceAnnotation
persistent identity: annotation2418009
display id: annotation2418009
version: 1
top level: BBa_K1486006
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1486006/annotation2418009/range2418009/1

https://synbiohub.org/public/igem/BBa_K1486006/annotation2418009/range2418009/1
type: Range
persistent identity: range2418009
display id: range2418009
version: 1
direction: direction/forward
top level: BBa_K1486006
start: 1
end: 3
orientation: inline

IFP[1]
type: SequenceAnnotation
persistent identity: annotation2418010
display id: annotation2418010
version: 1
top level: BBa_K1486006
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1486006/annotation2418010/range2418010/1

https://synbiohub.org/public/igem/BBa_K1486006/annotation2418010/range2418010/1
type: Range
persistent identity: range2418010
display id: range2418010
version: 1
direction: direction/forward
top level: BBa_K1486006
start: 1
end: 405
orientation: inline

STOP
type: SequenceAnnotation
persistent identity: annotation2418011
display id: annotation2418011
version: 1
top level: BBa_K1486006
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1486006/annotation2418011/range2418011/1

https://synbiohub.org/public/igem/BBa_K1486006/annotation2418011/range2418011/1
type: Range
persistent identity: range2418011
display id: range2418011
version: 1
direction: direction/forward
top level: BBa_K1486006
start: 403
end: 405
orientation: inline

BBa_K1122008
type: ComponentDefinition
persistent identity: BBa K1122008
display id: BBa_K1122008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: mTagBFP (BBa_K592100) compatible with Genabler assembly
creator: Lina Gasiūnaitė
created: 2013-08-12T11:00:00Z
modified: 2016-01-26T02:14:23Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1433_
m user id: 4206
owner id: 18510
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: mTagBFP (BBa_K592100) part with inward facing EarI recognition sites at the ends. It is compatible with Genabler assembly.
mutable notes: To be compatible with Genabler assembly, the part has inward-facing EarI sites at the ends. When assembled by BioBrick assembly, five N-terminal residues MetAlaSerSerSer- and one C-terminal Ala residue will be added to the original sequence of BBa_K592100.
mutable provenance: mTagBFP is based on the BioBrick BBa_K592100.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: mTagBFP
sequence annotation: stop
sequence annotation: EarI site
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_K1122008_sequence/1

mTagBFP
type: SequenceAnnotation
persistent identity: annotation2346269
display id: annotation2346269
version: 1
top level: BBa_K1122008
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1122008/annotation2346269/range2346269/1

https://synbiohub.org/public/igem/BBa_K1122008/annotation2346269/range2346269/1
type: Range
persistent identity: range2346269
display id: range2346269
version: 1
direction: direction/forward
top level: BBa_K1122008
start: 1
end: 721
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2346270
display id: annotation2346270
version: 1
top level: BBa_K1122008
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1122008/annotation2346270/range2346270/1

https://synbiohub.org/public/igem/BBa_K1122008/annotation2346270/range2346270/1
type: Range
persistent identity: range2346270
display id: range2346270
version: 1
direction: direction/forward
top level: BBa_K1122008
start: 716
end: 718
orientation: inline

EarI site
type: SequenceAnnotation
persistent identity: annotation2346268
display id: annotation2346268
version: 1
top level: BBa_K1122008
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1122008/annotation2346268/range2346268/1

https://synbiohub.org/public/igem/BBa_K1122008/annotation2346268/range2346268/1
type: Range
persistent identity: range2346268
display id: range2346268
version: 1
direction: direction/forward
top level: BBa_K1122008
start: 6
end: 11
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2346267
display id: annotation2346267
version: 1
top level: BBa_K1122008
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1122008/annotation2346267/range2346267/1

https://synbiohub.org/public/igem/BBa_K1122008/annotation2346267/range2346267/1
type: Range
persistent identity: range2346267
display id: range2346267
version: 1
direction: direction/forward
top level: BBa_K1122008
start: 1
end: 3
orientation: inline

EarI site
type: SequenceAnnotation
persistent identity: annotation2346271
display id: annotation2346271
version: 1
top level: BBa_K1122008
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1122008/annotation2346271/range2346271/1

https://synbiohub.org/public/igem/BBa_K1122008/annotation2346271/range2346271/1
type: Range
persistent identity: range2346271
display id: range2346271
version: 1
direction: direction/reverse
top level: BBa_K1122008
start: 716
end: 721
orientation: inline

BBa_Z0301
type: ComponentDefinition
persistent identity: BBa Z0301
display id: BBa_Z0301
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Venus YFP - E. coli codon optimized
creator: Sriram Kosuri
created: 2006-06-20T11:00:00Z
modified: 2015-05-08T01:14:55Z
discontinued: false
dominant: false
group u list: _11_
m user id: 0
owner id: 64
owning group id: 10
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Brighter Variant than standard EYFP.
mutable notes: None really
mutable provenance: Venus Sequence obtained from Prof. Miyawaki. Codon optimized from Gene Design by Boeke Lab.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: gtc ...gtt -> gta ... gtt codon shuffle
sequence: https://synbiohub.org/public/igem/BBa_Z0301_sequence/1

gtc ...gtt -> gta ... gtt codon shuffle
type: SequenceAnnotation
persistent identity: annotation1881736
display id: annotation1881736
version: 1
top level: BBa_Z0301
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_Z0301/annotation1881736/range1881736/1

https://synbiohub.org/public/igem/BBa_Z0301/annotation1881736/range1881736/1
type: Range
persistent identity: range1881736
display id: range1881736
version: 1
direction: direction/forward
top level: BBa_Z0301
start: 4
end: 39
orientation: inline

mCherry
type: ComponentDefinition
persistent identity: BBa J06504
display id: BBa_J06504
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: monomeric RFP optimized for bacteria
creator: ytwang
created: 2005-07-17T11:00:00Z
modified: 2016-01-25T01:05:28Z
discontinued: false
dominant: false
experience: experience/None
group u list: _20_
m user id: 4206
owner id: 340
owning group id: 20
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: mRFP (DsRed) derived, altered to be a biobrick by removing a PstI site and adding in the ends.
mutable notes: Made a point mutation to eliminate a PstI site in the middle and then added BioBrick ends using PCR. Sequenced using primers that bind to the pSB1A2 plasmid on either side of the brick.
mutable provenance: mCherry from Roger Y. Tsien's lab, altered to be BioBrick compatible
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: mCherry
sequence annotation: C->T (removing PstI site)
sequence: https://synbiohub.org/public/igem/BBa_J06504_sequence/1

mCherry
type: SequenceAnnotation
persistent identity: annotation1585829
display id: annotation1585829
version: 1
top level: mCherry
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J06504/annotation1585829/range1585829/1

https://synbiohub.org/public/igem/BBa_J06504/annotation1585829/range1585829/1
type: Range
persistent identity: range1585829
display id: range1585829
version: 1
direction: direction/forward
top level: mCherry
start: 1
end: 711
orientation: inline

C->T (removing PstI site)
type: SequenceAnnotation
persistent identity: annotation1585833
display id: annotation1585833
version: 1
top level: mCherry
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_J06504/annotation1585833/range1585833/1

https://synbiohub.org/public/igem/BBa_J06504/annotation1585833/range1585833/1
type: Range
persistent identity: range1585833
display id: range1585833
version: 1
direction: direction/forward
top level: mCherry
start: 352
end: 352
orientation: inline

SP-AntiNIP
type: ComponentDefinition
persistent identity: BBa K157028
display id: BBa_K157028
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: SP-AntiNIP-GGGSlinker-Tm-Fluolinker-cCFP
creator: iGEM Team Freiburg 2008
created: 2008-10-25T11:00:00Z
modified: 2015-05-08T01:10:55Z
discontinued: false
dominant: false
experience: experience/None
group u list: _232_
m user id: 0
owner id: 1673
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Part consists of the signal peptide of Erbb1/EGF-receptor, scFV-antiNIP, GGGSflexlinker (extracellular part), the trans-membrane region of the Erbb1/EGF-receptor and the C-terminal part of the split fluorophor YFP with a fluolinker joined to its N-Terminus (intracellular part).
mutable notes: none
mutable provenance: Derived by cloning of the parts BBa_K157001, BBa_K157003, BBa_K157013, BBa_K157002, BBa_K157009 and BBa_K157005. For further information see the description of the parts mentioned above.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: Scar (Thr, Gly)
sequence annotation: SP-AntiNIP-GGGSlinker-Tm-Fluolinker-cCFP
sequence annotation: GGGS-Linker
sequence annotation: Fluolinker
sequence annotation: NgoMI
sequence annotation: AgeI
sequence annotation: Sc-Fv_anti-NIP
sequence annotation: C-Term_CFP
sequence annotation: Tm
sequence annotation: SP
sequence: https://synbiohub.org/public/igem/BBa_K157028_sequence/1

Scar (Thr, Gly)
type: SequenceAnnotation
persistent identity: annotation1993298
display id: annotation1993298
version: 1
top level: SP-AntiNIP
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157028/annotation1993298/range1993298/1

https://synbiohub.org/public/igem/BBa_K157028/annotation1993298/range1993298/1
type: Range
persistent identity: range1993298
display id: range1993298
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 1006
end: 1011
orientation: inline

SP-AntiNIP-GGGSlinker-Tm-Fluolinker-cCFP
type: SequenceAnnotation
persistent identity: annotation1990209
display id: annotation1990209
version: 1
top level: SP-AntiNIP
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K157028/annotation1990209/range1990209/1

https://synbiohub.org/public/igem/BBa_K157028/annotation1990209/range1990209/1
type: Range
persistent identity: range1990209
display id: range1990209
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 1
end: 1260
orientation: inline

Scar (Thr, Gly)
type: SequenceAnnotation
persistent identity: annotation1993290
display id: annotation1993290
version: 1
top level: SP-AntiNIP
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157028/annotation1993290/range1993290/1

https://synbiohub.org/public/igem/BBa_K157028/annotation1993290/range1993290/1
type: Range
persistent identity: range1993290
display id: range1993290
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 82
end: 87
orientation: inline

GGGS-Linker
type: SequenceAnnotation
persistent identity: annotation1993293
display id: annotation1993293
version: 1
top level: SP-AntiNIP
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157028/annotation1993293/range1993293/1

https://synbiohub.org/public/igem/BBa_K157028/annotation1993293/range1993293/1
type: Range
persistent identity: range1993293
display id: range1993293
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 829
end: 873
orientation: inline

Fluolinker
type: SequenceAnnotation
persistent identity: annotation1993297
display id: annotation1993297
version: 1
top level: SP-AntiNIP
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157028/annotation1993297/range1993297/1

https://synbiohub.org/public/igem/BBa_K157028/annotation1993297/range1993297/1
type: Range
persistent identity: range1993297
display id: range1993297
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 955
end: 1005
orientation: inline

Scar (Thr, Gly)
type: SequenceAnnotation
persistent identity: annotation1993294
display id: annotation1993294
version: 1
top level: SP-AntiNIP
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157028/annotation1993294/range1993294/1

https://synbiohub.org/public/igem/BBa_K157028/annotation1993294/range1993294/1
type: Range
persistent identity: range1993294
display id: range1993294
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 874
end: 879
orientation: inline

NgoMI
type: SequenceAnnotation
persistent identity: annotation1990180
display id: annotation1990180
version: 1
top level: SP-AntiNIP
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157028/annotation1990180/range1990180/1

https://synbiohub.org/public/igem/BBa_K157028/annotation1990180/range1990180/1
type: Range
persistent identity: range1990180
display id: range1990180
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 4
end: 9
orientation: inline

Scar (Thr, Gly)
type: SequenceAnnotation
persistent identity: annotation1993292
display id: annotation1993292
version: 1
top level: SP-AntiNIP
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157028/annotation1993292/range1993292/1

https://synbiohub.org/public/igem/BBa_K157028/annotation1993292/range1993292/1
type: Range
persistent identity: range1993292
display id: range1993292
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 823
end: 828
orientation: inline

AgeI
type: SequenceAnnotation
persistent identity: annotation1990181
display id: annotation1990181
version: 1
top level: SP-AntiNIP
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157028/annotation1990181/range1990181/1

https://synbiohub.org/public/igem/BBa_K157028/annotation1990181/range1990181/1
type: Range
persistent identity: range1990181
display id: range1990181
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 1255
end: 1260
orientation: inline

Sc-Fv_anti-NIP
type: SequenceAnnotation
persistent identity: annotation1993291
display id: annotation1993291
version: 1
top level: SP-AntiNIP
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157028/annotation1993291/range1993291/1

https://synbiohub.org/public/igem/BBa_K157028/annotation1993291/range1993291/1
type: Range
persistent identity: range1993291
display id: range1993291
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 88
end: 822
orientation: inline

Scar (Thr, Gly)
type: SequenceAnnotation
persistent identity: annotation1993296
display id: annotation1993296
version: 1
top level: SP-AntiNIP
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157028/annotation1993296/range1993296/1

https://synbiohub.org/public/igem/BBa_K157028/annotation1993296/range1993296/1
type: Range
persistent identity: range1993296
display id: range1993296
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 949
end: 954
orientation: inline

C-Term_CFP
type: SequenceAnnotation
persistent identity: annotation1993299
display id: annotation1993299
version: 1
top level: SP-AntiNIP
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157028/annotation1993299/range1993299/1

https://synbiohub.org/public/igem/BBa_K157028/annotation1993299/range1993299/1
type: Range
persistent identity: range1993299
display id: range1993299
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 1012
end: 1254
orientation: inline

Tm
type: SequenceAnnotation
persistent identity: annotation1993295
display id: annotation1993295
version: 1
top level: SP-AntiNIP
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157028/annotation1993295/range1993295/1

https://synbiohub.org/public/igem/BBa_K157028/annotation1993295/range1993295/1
type: Range
persistent identity: range1993295
display id: range1993295
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 880
end: 948
orientation: inline

SP
type: SequenceAnnotation
persistent identity: annotation1993289
display id: annotation1993289
version: 1
top level: SP-AntiNIP
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157028/annotation1993289/range1993289/1

https://synbiohub.org/public/igem/BBa_K157028/annotation1993289/range1993289/1
type: Range
persistent identity: range1993289
display id: range1993289
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 10
end: 81
orientation: inline

BBa_K1399014
type: ComponentDefinition
persistent identity: BBa K1399014
display id: BBa_K1399014
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Plac-GFP(AAV)
creator: Anna Stikane
created: 2014-09-18T11:00:00Z
modified: 2015-05-08T01:10:16Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1777_
m user id: 0
owner id: 22477
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: Lactose/IPTG inducible promoter with GFP reporter tagged with AAV-ssrA degradation tag followed by terminator. The tagged GFP is actively degraded within cell, thus provides better temporal resolution of green fluorescence and promoter activity.
mutable notes: Part was assembled using BrickClip assembly (BBF RFC104) using other biobrick parts as templates. BrickClip assembly is a special case of more general Paperclip assembly method.[1]
mutable provenance: other biobricks
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_B0034
component: BBa_R0010
component: BBa_B0015
component: BBa_K1399005
sequence annotation: BBa_B0015
sequence annotation: BBa_R0010
sequence annotation: BBa_B0034
sequence annotation: BBa_K1399005
sequence: https://synbiohub.org/public/igem/BBa_K1399014_sequence/1

BBa_B0034
type: Component
persistent identity: component2384049
display id: component2384049
version: 1
top level: BBa_K1399014
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_R0010
type: Component
persistent identity: component2384041
display id: component2384041
version: 1
top level: BBa_K1399014
access: public
definition: https://synbiohub.org/public/igem/BBa_R0010/1

BBa_B0015
type: Component
persistent identity: component2384062
display id: component2384062
version: 1
top level: BBa_K1399014
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_K1399005
type: Component
persistent identity: component2384055
display id: component2384055
version: 1
top level: BBa_K1399014
access: public
definition: BBa_K1399005

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2384062
display id: annotation2384062
version: 1
top level: BBa_K1399014
location: https://synbiohub.org/public/igem/BBa_K1399014/annotation2384062/range2384062/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K1399014/annotation2384062/range2384062/1
type: Range
persistent identity: range2384062
display id: range2384062
version: 1
direction: direction/reverse
top level: BBa_K1399014
start: 988
end: 1116
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation2384041
display id: annotation2384041
version: 1
top level: BBa_K1399014
location: https://synbiohub.org/public/igem/BBa_K1399014/annotation2384041/range2384041/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_K1399014/annotation2384041/range2384041/1
type: Range
persistent identity: range2384041
display id: range2384041
version: 1
direction: direction/forward
top level: BBa_K1399014
start: 1
end: 200
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2384049
display id: annotation2384049
version: 1
top level: BBa_K1399014
location: https://synbiohub.org/public/igem/BBa_K1399014/annotation2384049/range2384049/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1399014/annotation2384049/range2384049/1
type: Range
persistent identity: range2384049
display id: range2384049
version: 1
direction: direction/reverse
top level: BBa_K1399014
start: 209
end: 220
orientation: inline

BBa_K1399005
type: SequenceAnnotation
persistent identity: annotation2384055
display id: annotation2384055
version: 1
top level: BBa_K1399014
location: https://synbiohub.org/public/igem/BBa_K1399014/annotation2384055/range2384055/1
component: BBa_K1399005

https://synbiohub.org/public/igem/BBa_K1399014/annotation2384055/range2384055/1
type: Range
persistent identity: range2384055
display id: range2384055
version: 1
direction: direction/reverse
top level: BBa_K1399014
start: 227
end: 979
orientation: inline

BBa_K1486012
type: ComponentDefinition
persistent identity: BBa K1486012
display id: BBa_K1486012
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CpxR-IFP[1] under pBAD
creator: iGem EPFL 2014
created: 2014-08-31T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part has a fused protein downstream of an arabinose-inducible promoter: CpxR-IFP[1]. Biobrick BBa_K1486013 has CpxR-IFP[2] with an arabinose promoter. This construct was made in both orientations: BBa_K1486014 is IFP[1]-CpxR (with an arabinose promoter).
mutable notes: Both orientations were made, as well as for IFP[2] so all dimerizations could be tested (see main page for biobrick numbers). Flexible linkers were used to fuse both proteins, and an RBS was put before each one of them.
mutable provenance: To Edit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0034
component: BBa_K1486000
component: BBa_I0500
component: BBa_K1486004
component: BBa_K1486006
sequence annotation: BBa_I0500
sequence annotation: BBa_B0034
sequence annotation: BBa_K1486004
sequence annotation: BBa_K1486000
sequence annotation: BBa_K1486006
sequence: https://synbiohub.org/public/igem/BBa_K1486012_sequence/1

BBa_B0034
type: Component
persistent identity: component2382287
display id: component2382287
version: 1
top level: BBa_K1486012
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K1486000
type: Component
persistent identity: component2382291
display id: component2382291
version: 1
top level: BBa_K1486012
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486000/1

BBa_I0500
type: Component
persistent identity: component2382285
display id: component2382285
version: 1
top level: BBa_K1486012
access: public
definition: https://synbiohub.org/public/igem/BBa_I0500/1

BBa_K1486004
type: Component
persistent identity: component2382292
display id: component2382292
version: 1
top level: BBa_K1486012
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486004/1

BBa_K1486006
type: Component
persistent identity: component2382293
display id: component2382293
version: 1
top level: BBa_K1486012
access: public
definition: BBa_K1486006

BBa_I0500
type: SequenceAnnotation
persistent identity: annotation2382285
display id: annotation2382285
version: 1
top level: BBa_K1486012
location: https://synbiohub.org/public/igem/BBa_K1486012/annotation2382285/range2382285/1
component: BBa_I0500

https://synbiohub.org/public/igem/BBa_K1486012/annotation2382285/range2382285/1
type: Range
persistent identity: range2382285
display id: range2382285
version: 1
direction: direction/reverse
top level: BBa_K1486012
start: 1
end: 1210
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2382287
display id: annotation2382287
version: 1
top level: BBa_K1486012
location: https://synbiohub.org/public/igem/BBa_K1486012/annotation2382287/range2382287/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1486012/annotation2382287/range2382287/1
type: Range
persistent identity: range2382287
display id: range2382287
version: 1
direction: direction/reverse
top level: BBa_K1486012
start: 1211
end: 1222
orientation: inline

BBa_K1486004
type: SequenceAnnotation
persistent identity: annotation2382292
display id: annotation2382292
version: 1
top level: BBa_K1486012
location: https://synbiohub.org/public/igem/BBa_K1486012/annotation2382292/range2382292/1
component: BBa_K1486004

https://synbiohub.org/public/igem/BBa_K1486012/annotation2382292/range2382292/1
type: Range
persistent identity: range2382292
display id: range2382292
version: 1
direction: direction/reverse
top level: BBa_K1486012
start: 1925
end: 1954
orientation: inline

BBa_K1486000
type: SequenceAnnotation
persistent identity: annotation2382291
display id: annotation2382291
version: 1
top level: BBa_K1486012
location: https://synbiohub.org/public/igem/BBa_K1486012/annotation2382291/range2382291/1
component: BBa_K1486000

https://synbiohub.org/public/igem/BBa_K1486012/annotation2382291/range2382291/1
type: Range
persistent identity: range2382291
display id: range2382291
version: 1
direction: direction/reverse
top level: BBa_K1486012
start: 1223
end: 1924
orientation: inline

BBa_K1486006
type: SequenceAnnotation
persistent identity: annotation2382293
display id: annotation2382293
version: 1
top level: BBa_K1486012
location: https://synbiohub.org/public/igem/BBa_K1486012/annotation2382293/range2382293/1
component: BBa_K1486006

https://synbiohub.org/public/igem/BBa_K1486012/annotation2382293/range2382293/1
type: Range
persistent identity: range2382293
display id: range2382293
version: 1
direction: direction/reverse
top level: BBa_K1486012
start: 1955
end: 2536
orientation: inline

BBa_K1486002
type: ComponentDefinition
persistent identity: BBa K1486002
display id: BBa_K1486002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Arabinose promoter + sfGFP CpxR (Nterm)
creator: iGem EPFL 2014
created: 2014-08-11T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part is composed of an arabinose-inducible promoter for superfolded GFP attached by a linker to CpxR (on the N terminus of CpxR).
mutable notes: We had to test both attachments, N and C terminus of the CpxR.
mutable provenance: The arabinose promoter and the sfGFP were obtained from the registry, while the CpxR was obtained from the E coli genome.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0034
component: BBa_I0500
component: BBa_K1486000
component: BBa_I746916
component: BBa_K1486003
sequence annotation: BBa_I746916
sequence annotation: BBa_B0034
sequence annotation: BBa_I0500
sequence annotation: BBa_K1486000
sequence annotation: BBa_K1486003
sequence: https://synbiohub.org/public/igem/BBa_K1486002_sequence/1

BBa_B0034
type: Component
persistent identity: component2382059
display id: component2382059
version: 1
top level: BBa_K1486002
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_I0500
type: Component
persistent identity: component2382057
display id: component2382057
version: 1
top level: BBa_K1486002
access: public
definition: https://synbiohub.org/public/igem/BBa_I0500/1

BBa_K1486000
type: Component
persistent identity: component2382068
display id: component2382068
version: 1
top level: BBa_K1486002
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486000/1

BBa_I746916
type: Component
persistent identity: component2382063
display id: component2382063
version: 1
top level: BBa_K1486002
access: public
definition: https://synbiohub.org/public/igem/BBa_I746916/1

BBa_K1486003
type: Component
persistent identity: component2382064
display id: component2382064
version: 1
top level: BBa_K1486002
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486003/1

BBa_I746916
type: SequenceAnnotation
persistent identity: annotation2382063
display id: annotation2382063
version: 1
top level: BBa_K1486002
location: https://synbiohub.org/public/igem/BBa_K1486002/annotation2382063/range2382063/1
component: BBa_I746916

https://synbiohub.org/public/igem/BBa_K1486002/annotation2382063/range2382063/1
type: Range
persistent identity: range2382063
display id: range2382063
version: 1
direction: direction/reverse
top level: BBa_K1486002
start: 1223
end: 1942
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2382059
display id: annotation2382059
version: 1
top level: BBa_K1486002
location: https://synbiohub.org/public/igem/BBa_K1486002/annotation2382059/range2382059/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1486002/annotation2382059/range2382059/1
type: Range
persistent identity: range2382059
display id: range2382059
version: 1
direction: direction/reverse
top level: BBa_K1486002
start: 1211
end: 1222
orientation: inline

BBa_I0500
type: SequenceAnnotation
persistent identity: annotation2382057
display id: annotation2382057
version: 1
top level: BBa_K1486002
location: https://synbiohub.org/public/igem/BBa_K1486002/annotation2382057/range2382057/1
component: BBa_I0500

https://synbiohub.org/public/igem/BBa_K1486002/annotation2382057/range2382057/1
type: Range
persistent identity: range2382057
display id: range2382057
version: 1
direction: direction/reverse
top level: BBa_K1486002
start: 1
end: 1210
orientation: inline

BBa_K1486000
type: SequenceAnnotation
persistent identity: annotation2382068
display id: annotation2382068
version: 1
top level: BBa_K1486002
location: https://synbiohub.org/public/igem/BBa_K1486002/annotation2382068/range2382068/1
component: BBa_K1486000

https://synbiohub.org/public/igem/BBa_K1486002/annotation2382068/range2382068/1
type: Range
persistent identity: range2382068
display id: range2382068
version: 1
direction: direction/reverse
top level: BBa_K1486002
start: 1967
end: 2668
orientation: inline

BBa_K1486003
type: SequenceAnnotation
persistent identity: annotation2382064
display id: annotation2382064
version: 1
top level: BBa_K1486002
location: https://synbiohub.org/public/igem/BBa_K1486002/annotation2382064/range2382064/1
component: BBa_K1486003

https://synbiohub.org/public/igem/BBa_K1486002/annotation2382064/range2382064/1
type: Range
persistent identity: range2382064
display id: range2382064
version: 1
direction: direction/reverse
top level: BBa_K1486002
start: 1943
end: 1966
orientation: inline

BBa_K2003010
type: ComponentDefinition
persistent identity: BBa K2003010
display id: BBa_K2003010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Inducible Green Fluorescent Protein UnaG+6xHis-tag
creator: Philip L??fgren
created: 2016-08-31T11:00:00Z
modified: 2016-10-16T06:08:47Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2470_
m user id: 31672
owner id: 31672
owning group id: 9
status: status/Planning
bookmark: false
mutable description: WiP
mutable notes: Potato
mutable provenance: Eel
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Hexahistidine tag
sequence annotation: 2x Stop codons
sequence annotation: Start
sequence annotation: Untranscribed flexible linker
sequence annotation: UnaG
sequence: https://synbiohub.org/public/igem/BBa_K2003010_sequence/1

Hexahistidine tag
type: SequenceAnnotation
persistent identity: annotation2512838
display id: annotation2512838
version: 1
top level: BBa_K2003010
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K2003010/annotation2512838/range2512838/1

https://synbiohub.org/public/igem/BBa_K2003010/annotation2512838/range2512838/1
type: Range
persistent identity: range2512838
display id: range2512838
version: 1
direction: direction/forward
top level: BBa_K2003010
start: 4
end: 18
orientation: inline

2x Stop codons
type: SequenceAnnotation
persistent identity: annotation2512840
display id: annotation2512840
version: 1
top level: BBa_K2003010
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K2003010/annotation2512840/range2512840/1

https://synbiohub.org/public/igem/BBa_K2003010/annotation2512840/range2512840/1
type: Range
persistent identity: range2512840
display id: range2512840
version: 1
direction: direction/forward
top level: BBa_K2003010
start: 439
end: 444
orientation: inline

Start
type: SequenceAnnotation
persistent identity: annotation2512842
display id: annotation2512842
version: 1
top level: BBa_K2003010
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K2003010/annotation2512842/range2512842/1

https://synbiohub.org/public/igem/BBa_K2003010/annotation2512842/range2512842/1
type: Range
persistent identity: range2512842
display id: range2512842
version: 1
direction: direction/forward
top level: BBa_K2003010
start: 1
end: 3
orientation: inline

Untranscribed flexible linker
type: SequenceAnnotation
persistent identity: annotation2512841
display id: annotation2512841
version: 1
top level: BBa_K2003010
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K2003010/annotation2512841/range2512841/1

https://synbiohub.org/public/igem/BBa_K2003010/annotation2512841/range2512841/1
type: Range
persistent identity: range2512841
display id: range2512841
version: 1
direction: direction/forward
top level: BBa_K2003010
start: 445
end: 462
orientation: inline

UnaG
type: SequenceAnnotation
persistent identity: annotation2512839
display id: annotation2512839
version: 1
top level: BBa_K2003010
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K2003010/annotation2512839/range2512839/1

https://synbiohub.org/public/igem/BBa_K2003010/annotation2512839/range2512839/1
type: Range
persistent identity: range2512839
display id: range2512839
version: 1
direction: direction/forward
top level: BBa_K2003010
start: 19
end: 439
orientation: inline

BBa_E3030
type: ComponentDefinition
persistent identity: BBa E3030
display id: BBa_E3030
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: FRET acceptor YPet (optimized to work with CyPet (Cyan FP) as donor
creator: ryu
created: 2005-03-11T12:00:00Z
modified: 2015-08-31T04:07:27Z
discontinued: false
dominant: false
experience: experience/None
group u list: _8_
m user id: 0
owner id: 230
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: YPet YFP, optimized CyPet (Cyan FP) FRET acceptor
mutable notes: Contains A206K (in this sequence A211K due to N-terminal linker) mutation to reduce dimerization potential as described by Roger Tsien. Contains N- and C-terminal linker sequences (with homology to biobrick parts BBa_E2060 (mCherry), BBa_E2050 (mOrange), and BBa_E2020 (Cerulean CFP) to facilitate color swapping in yeast. Adds N-"MATSG" and "GSGTA"-C. to published amino acid sequence. Inserts a "V" after normal start M. Double TAATAA stop codon. Missing EcoRI, HindIII, NotI, NdeI, XhoI, RsrII, BamHI, NcoI, BglI, SpeI, XbaI, and PstI. Except for 5' and 3' ends no significant sequence identity runs with above biobrick FPs and GFP S65T as found in O'Shea deletion strain collection (originally from plasmid pFA6-GFP(S65T)-His3MX6).
mutable provenance: Nguyen et al, (2005) Nature Biotech Feb 6 Epub PMID: 15696158
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: GSGTA linker
sequence annotation: Help:Barcodes
sequence annotation: MATSG linker
sequence: https://synbiohub.org/public/igem/BBa_E3030_sequence/1

GSGTA linker
type: SequenceAnnotation
persistent identity: annotation1436042
display id: annotation1436042
version: 1
top level: BBa_E3030
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_E3030/annotation1436042/range1436042/1

https://synbiohub.org/public/igem/BBa_E3030/annotation1436042/range1436042/1
type: Range
persistent identity: range1436042
display id: range1436042
version: 1
direction: direction/forward
top level: BBa_E3030
start: 733
end: 747
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2214025
display id: annotation2214025
version: 1
top level: BBa_E3030
role: SO:0000807
role: feature/barcode
location: https://synbiohub.org/public/igem/BBa_E3030/annotation2214025/range2214025/1

https://synbiohub.org/public/igem/BBa_E3030/annotation2214025/range2214025/1
type: Range
persistent identity: range2214025
display id: range2214025
version: 1
direction: direction/forward
top level: BBa_E3030
start: 754
end: 778
orientation: inline

MATSG linker
type: SequenceAnnotation
persistent identity: annotation1436041
display id: annotation1436041
version: 1
top level: BBa_E3030
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_E3030/annotation1436041/range1436041/1

https://synbiohub.org/public/igem/BBa_E3030/annotation1436041/range1436041/1
type: Range
persistent identity: range1436041
display id: range1436041
version: 1
direction: direction/forward
top level: BBa_E3030
start: 1
end: 15
orientation: inline

dTomato
type: ComponentDefinition
persistent identity: BBa K1688019
display id: BBa_K1688019
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: dTomato, red fluorescent protein
creator: Erik Gullberg
created: 2015-09-14T11:00:00Z
modified: 2015-09-15T12:53:18Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2106_
m user id: 27118
owner id: 27118
owning group id: 9
status: status/Planning
bookmark: false
mutable description: The dTomato protein is a fluorescent dimer protein that emits orange-red light when it is excited by green-yellow light. It is preferable to use ??? especially in self-made fluorometry tests ??? because the excitation wavelengths and the emission wavelengths do not overlap as much as in other fluorescent proteins. The dTomato excitation peak is at 554 nm and 50% of it is at 510 nm. The emission peak is at 581 nm and the 50% emission peak is at 629 nm.
mutable provenance: dTomato was derived from a monomeric mutant of the anthozoan coral RFP, DsRed.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1688019_sequence/1

BBa_K352017
type: ComponentDefinition
persistent identity: BBa K352017
display id: BBa_K352017
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pCooM RBS RFP Double terminator CooA coupled with placI+RBS
creator: Ozkan Is
created: 2010-10-26T11:00:00Z
modified: 2015-05-08T01:12:11Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _474_
m user id: 0
owner id: 6078
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: This part is formed by combination of pCooM Promoter coupled with RBS+RFP+Double terminator and CooA coupled with placI+RBS.
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_K352014
component: BBa_K352011
sequence annotation: BBa_K352014
sequence annotation: BBa_K352011
sequence: https://synbiohub.org/public/igem/BBa_K352017_sequence/1

BBa_K352014
type: Component
persistent identity: component2273378
display id: component2273378
version: 1
top level: BBa_K352017
access: public
definition: https://synbiohub.org/public/igem/BBa_K352014/1

BBa_K352011
type: Component
persistent identity: component2273366
display id: component2273366
version: 1
top level: BBa_K352017
access: public
definition: https://synbiohub.org/public/igem/BBa_K352011/1

BBa_K352014
type: SequenceAnnotation
persistent identity: annotation2273378
display id: annotation2273378
version: 1
top level: BBa_K352017
location: https://synbiohub.org/public/igem/BBa_K352017/annotation2273378/range2273378/1
component: BBa_K352014

https://synbiohub.org/public/igem/BBa_K352017/annotation2273378/range2273378/1
type: Range
persistent identity: range2273378
display id: range2273378
version: 1
direction: direction/reverse
top level: BBa_K352017
start: 944
end: 1838
orientation: inline

BBa_K352011
type: SequenceAnnotation
persistent identity: annotation2273366
display id: annotation2273366
version: 1
top level: BBa_K352017
location: https://synbiohub.org/public/igem/BBa_K352017/annotation2273366/range2273366/1
component: BBa_K352011

https://synbiohub.org/public/igem/BBa_K352017/annotation2273366/range2273366/1
type: Range
persistent identity: range2273366
display id: range2273366
version: 1
direction: direction/reverse
top level: BBa_K352017
start: 1
end: 935
orientation: inline

BBa_K1031114
type: ComponentDefinition
persistent identity: BBa K1031114
display id: BBa_K1031114
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Po-B0032-sfGFP (CapR)
creator: He Shuaixin
created: 2013-09-08T11:00:00Z
modified: 2015-05-08T01:08:47Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1338_
m user id: 0
owner id: 15843
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Po-32-sfGFP (CapR)
mutable notes: molecular cloning
mutable provenance: synthesis, lab made
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: B0032
sequence annotation: Po (CapR)
sequence annotation: B0015
sequence annotation: sfGFP
sequence: https://synbiohub.org/public/igem/BBa_K1031114_sequence/1

B0032
type: SequenceAnnotation
persistent identity: annotation2362069
display id: annotation2362069
version: 1
top level: BBa_K1031114
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1031114/annotation2362069/range2362069/1

https://synbiohub.org/public/igem/BBa_K1031114/annotation2362069/range2362069/1
type: Range
persistent identity: range2362069
display id: range2362069
version: 1
direction: direction/forward
top level: BBa_K1031114
start: 177
end: 189
orientation: inline

Po (CapR)
type: SequenceAnnotation
persistent identity: annotation2362068
display id: annotation2362068
version: 1
top level: BBa_K1031114
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1031114/annotation2362068/range2362068/1

https://synbiohub.org/public/igem/BBa_K1031114/annotation2362068/range2362068/1
type: Range
persistent identity: range2362068
display id: range2362068
version: 1
direction: direction/forward
top level: BBa_K1031114
start: 1
end: 170
orientation: inline

B0015
type: SequenceAnnotation
persistent identity: annotation2362071
display id: annotation2362071
version: 1
top level: BBa_K1031114
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1031114/annotation2362071/range2362071/1

https://synbiohub.org/public/igem/BBa_K1031114/annotation2362071/range2362071/1
type: Range
persistent identity: range2362071
display id: range2362071
version: 1
direction: direction/forward
top level: BBa_K1031114
start: 925
end: 1053
orientation: inline

sfGFP
type: SequenceAnnotation
persistent identity: annotation2362070
display id: annotation2362070
version: 1
top level: BBa_K1031114
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1031114/annotation2362070/range2362070/1

https://synbiohub.org/public/igem/BBa_K1031114/annotation2362070/range2362070/1
type: Range
persistent identity: range2362070
display id: range2362070
version: 1
direction: direction/forward
top level: BBa_K1031114
start: 196
end: 918
orientation: inline

BBa_K156009
type: ComponentDefinition
persistent identity: BBa K156009
display id: BBa_K156009
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: OFP (orange fluorescent protein)
creator: George McArthur IV
created: 2008-10-27T12:00:00Z
modified: 2015-05-08T01:10:54Z
discontinued: false
dominant: false
experience: experience/None
group u list: _246_
m user id: 0
owner id: 1475
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Fluorescent proteins are useful tools for visualizing and quantifying protein expression in cells. OFP is yet another fluorescent protein, like GFP, that can be utilized as a reporter.
mutable notes: This part was codon-optimized for expression in E. coli. The following restriction enzyme sites were also removed: EcoRI, NotI, XbaI, SpeI, PstI, NheI, AvrII, ApoI, MfeI, NsiI, SbfI.
mutable provenance: OFP was cloned from the tentacles of Cerianthus sp., a tube anemone. The original sequence was obtained from GenBank (VERSION AY296063.1, GI:31616578).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K156009_sequence/1

BBa_K863113
type: ComponentDefinition
persistent identity: BBa K863113
display id: BBa_K863113
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Cellulose binding Domain of C. cellulovorans cellulose binding protein with Reporter GFP
creator: Moritz M??ller
created: 2012-09-19T11:00:00Z
modified: 2015-06-17T01:20:29Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1123_
m user id: 4206
owner id: 13080
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Cellulose binding Domain from Clostridium cellulovorans [http://www.ncbi.nlm.nih.gov/nuccore/M73817 cellulose binding protein gene] with T7, RBS linked with two amino acids (GS) to a GFP with His-tag.
mutable notes: CBDclos fused to a his-tagged GFP in a Freiburg-manner
mutable provenance: Cloning-vector and [http://partsregistry.org/Part:BBa_K863121 BBa_K863121]
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: BBa_E0040
sequence annotation: TAA
sequence annotation: GS-Linker
sequence annotation: Cellulose binding domain (CBDclos)
sequence annotation: Silent mutation (A original)
sequence annotation: T7 promoter
sequence annotation: ATG
sequence annotation: BBa_K863112
sequence annotation: BBa_K863111
sequence annotation: His-Tag
sequence annotation: Cellulose Binding Protein
sequence annotation: RBS
sequence annotation: BBa_K863121
sequence annotation: B0034
sequence annotation: Freiburg-Scar
sequence annotation: BBa_K525998
sequence annotation: AgeI
sequence: https://synbiohub.org/public/igem/BBa_K863113_sequence/1

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation2188399
display id: annotation2188399
version: 1
top level: BBa_K863113
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K863113/annotation2188399/range2188399/1

https://synbiohub.org/public/igem/BBa_K863113/annotation2188399/range2188399/1
type: Range
persistent identity: range2188399
display id: range2188399
version: 1
direction: direction/forward
top level: BBa_K863113
start: 335
end: 1048
orientation: inline

TAA
type: SequenceAnnotation
persistent identity: annotation2188402
display id: annotation2188402
version: 1
top level: BBa_K863113
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K863113/annotation2188402/range2188402/1

https://synbiohub.org/public/igem/BBa_K863113/annotation2188402/range2188402/1
type: Range
persistent identity: range2188402
display id: range2188402
version: 1
direction: direction/forward
top level: BBa_K863113
start: 1073
end: 1075
orientation: inline

GS-Linker
type: SequenceAnnotation
persistent identity: annotation2188396
display id: annotation2188396
version: 1
top level: BBa_K863113
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K863113/annotation2188396/range2188396/1

https://synbiohub.org/public/igem/BBa_K863113/annotation2188396/range2188396/1
type: Range
persistent identity: range2188396
display id: range2188396
version: 1
direction: direction/forward
top level: BBa_K863113
start: 323
end: 328
orientation: inline

Cellulose binding domain (CBDclos)
type: SequenceAnnotation
persistent identity: annotation2188394
display id: annotation2188394
version: 1
top level: BBa_K863113
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K863113/annotation2188394/range2188394/1

https://synbiohub.org/public/igem/BBa_K863113/annotation2188394/range2188394/1
type: Range
persistent identity: range2188394
display id: range2188394
version: 1
direction: direction/forward
top level: BBa_K863113
start: 41
end: 316
orientation: inline

Silent mutation (A original)
type: SequenceAnnotation
persistent identity: annotation2200501
display id: annotation2200501
version: 1
top level: BBa_K863113
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_K863113/annotation2200501/range2200501/1

https://synbiohub.org/public/igem/BBa_K863113/annotation2200501/range2200501/1
type: Range
persistent identity: range2200501
display id: range2200501
version: 1
direction: direction/forward
top level: BBa_K863113
start: 112
end: 112
orientation: inline

T7 promoter
type: SequenceAnnotation
persistent identity: annotation2188388
display id: annotation2188388
version: 1
top level: BBa_K863113
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K863113/annotation2188388/range2188388/1

https://synbiohub.org/public/igem/BBa_K863113/annotation2188388/range2188388/1
type: Range
persistent identity: range2188388
display id: range2188388
version: 1
direction: direction/forward
top level: BBa_K863113
start: 1
end: 20
orientation: inline

ATG
type: SequenceAnnotation
persistent identity: annotation2188391
display id: annotation2188391
version: 1
top level: BBa_K863113
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K863113/annotation2188391/range2188391/1

https://synbiohub.org/public/igem/BBa_K863113/annotation2188391/range2188391/1
type: Range
persistent identity: range2188391
display id: range2188391
version: 1
direction: direction/forward
top level: BBa_K863113
start: 35
end: 38
orientation: inline

BBa_K863112
type: SequenceAnnotation
persistent identity: annotation2188386
display id: annotation2188386
version: 1
top level: BBa_K863113
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K863113/annotation2188386/range2188386/1

https://synbiohub.org/public/igem/BBa_K863113/annotation2188386/range2188386/1
type: Range
persistent identity: range2188386
display id: range2188386
version: 1
direction: direction/reverse
top level: BBa_K863113
start: 1
end: 328
orientation: inline

BBa_K863111
type: SequenceAnnotation
persistent identity: annotation2188393
display id: annotation2188393
version: 1
top level: BBa_K863113
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K863113/annotation2188393/range2188393/1

https://synbiohub.org/public/igem/BBa_K863113/annotation2188393/range2188393/1
type: Range
persistent identity: range2188393
display id: range2188393
version: 1
direction: direction/forward
top level: BBa_K863113
start: 35
end: 328
orientation: inline

His-Tag
type: SequenceAnnotation
persistent identity: annotation2188400
display id: annotation2188400
version: 1
top level: BBa_K863113
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K863113/annotation2188400/range2188400/1

https://synbiohub.org/public/igem/BBa_K863113/annotation2188400/range2188400/1
type: Range
persistent identity: range2188400
display id: range2188400
version: 1
direction: direction/forward
top level: BBa_K863113
start: 1049
end: 1066
orientation: inline

Cellulose Binding Protein
type: SequenceAnnotation
persistent identity: annotation2188395
display id: annotation2188395
version: 1
top level: BBa_K863113
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K863113/annotation2188395/range2188395/1

https://synbiohub.org/public/igem/BBa_K863113/annotation2188395/range2188395/1
type: Range
persistent identity: range2188395
display id: range2188395
version: 1
direction: direction/forward
top level: BBa_K863113
start: 317
end: 322
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2188390
display id: annotation2188390
version: 1
top level: BBa_K863113
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K863113/annotation2188390/range2188390/1

https://synbiohub.org/public/igem/BBa_K863113/annotation2188390/range2188390/1
type: Range
persistent identity: range2188390
display id: range2188390
version: 1
direction: direction/forward
top level: BBa_K863113
start: 24
end: 27
orientation: inline

BBa_K863121
type: SequenceAnnotation
persistent identity: annotation2188398
display id: annotation2188398
version: 1
top level: BBa_K863113
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K863113/annotation2188398/range2188398/1

https://synbiohub.org/public/igem/BBa_K863113/annotation2188398/range2188398/1
type: Range
persistent identity: range2188398
display id: range2188398
version: 1
direction: direction/reverse
top level: BBa_K863113
start: 335
end: 1075
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation2188389
display id: annotation2188389
version: 1
top level: BBa_K863113
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K863113/annotation2188389/range2188389/1

https://synbiohub.org/public/igem/BBa_K863113/annotation2188389/range2188389/1
type: Range
persistent identity: range2188389
display id: range2188389
version: 1
direction: direction/forward
top level: BBa_K863113
start: 21
end: 32
orientation: inline

Cellulose Binding Protein
type: SequenceAnnotation
persistent identity: annotation2188392
display id: annotation2188392
version: 1
top level: BBa_K863113
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K863113/annotation2188392/range2188392/1

https://synbiohub.org/public/igem/BBa_K863113/annotation2188392/range2188392/1
type: Range
persistent identity: range2188392
display id: range2188392
version: 1
direction: direction/forward
top level: BBa_K863113
start: 35
end: 40
orientation: inline

Freiburg-Scar
type: SequenceAnnotation
persistent identity: annotation2188397
display id: annotation2188397
version: 1
top level: BBa_K863113
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K863113/annotation2188397/range2188397/1

https://synbiohub.org/public/igem/BBa_K863113/annotation2188397/range2188397/1
type: Range
persistent identity: range2188397
display id: range2188397
version: 1
direction: direction/forward
top level: BBa_K863113
start: 329
end: 334
orientation: inline

BBa_K525998
type: SequenceAnnotation
persistent identity: annotation2188387
display id: annotation2188387
version: 1
top level: BBa_K863113
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K863113/annotation2188387/range2188387/1

https://synbiohub.org/public/igem/BBa_K863113/annotation2188387/range2188387/1
type: Range
persistent identity: range2188387
display id: range2188387
version: 1
direction: direction/forward
top level: BBa_K863113
start: 1
end: 32
orientation: inline

AgeI
type: SequenceAnnotation
persistent identity: annotation2188401
display id: annotation2188401
version: 1
top level: BBa_K863113
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K863113/annotation2188401/range2188401/1

https://synbiohub.org/public/igem/BBa_K863113/annotation2188401/range2188401/1
type: Range
persistent identity: range2188401
display id: range2188401
version: 1
direction: direction/forward
top level: BBa_K863113
start: 1068
end: 1072
orientation: inline

BBa_K1650038
type: ComponentDefinition
persistent identity: BBa K1650038
display id: BBa_K1650038
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: GFP-His
creator: Alexandra Richter
created: 2015-09-17T11:00:00Z
modified: 2016-01-21T02:24:59Z
discontinued: false
dominant: false
group u list: _2067_
m user id: 4206
owner id: 27246
owning group id: 9
status: status/Planning
bookmark: false
mutable description: test
mutable notes: test
mutable provenance: test
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: BBa_K1650038
sequence: https://synbiohub.org/public/igem/BBa_K1650038_sequence/1

BBa_K1650038
type: SequenceAnnotation
persistent identity: annotation2474934
display id: annotation2474934
version: 1
top level: BBa_K1650038
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1650038/annotation2474934/range2474934/1

https://synbiohub.org/public/igem/BBa_K1650038/annotation2474934/range2474934/1
type: Range
persistent identity: range2474934
display id: range2474934
version: 1
direction: direction/forward
top level: BBa_K1650038
start: 1
end: 1159
orientation: inline

dTomato
type: ComponentDefinition
persistent identity: BBa K1688011
display id: BBa_K1688011
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: dTomato (inc RBS, J23110 promoter)
creator: Dianna Zeleskov
created: 2015-09-14T11:00:00Z
modified: 2015-09-18T11:31:20Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2106_
m user id: 27796
owner id: 27121
owning group id: 9
status: status/Planning
bookmark: false
mutable description: '''The dTomato protein is a fluorescent dimer protein that emits orange-red light when it is excited by green-yellow light. The excitation wavelengths and the emission wavelengths don't overlap as much as in other fluorescent proteins, which makes it preferable to use ??? especially in self‑made fluorometry tests.'''
mutable provenance: dTomato was derived from a monomeric mutant of the anthozoan coral RFP, DsRed.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_K1688004
component: BBa_J23110
sequence annotation: BBa_K1688004
sequence annotation: BBa_J23110
sequence: https://synbiohub.org/public/igem/BBa_K1688011_sequence/1

BBa_K1688004
type: Component
persistent identity: component2457939
display id: component2457939
version: 1
top level: dTomato
access: public
definition: dTomato

BBa_J23110
type: Component
persistent identity: component2457935
display id: component2457935
version: 1
top level: dTomato
access: public
definition: https://synbiohub.org/public/igem/BBa_J23110/1

BBa_K1688004
type: SequenceAnnotation
persistent identity: annotation2457939
display id: annotation2457939
version: 1
top level: dTomato
location: https://synbiohub.org/public/igem/BBa_K1688011/annotation2457939/range2457939/1
component: BBa_K1688004

https://synbiohub.org/public/igem/BBa_K1688011/annotation2457939/range2457939/1
type: Range
persistent identity: range2457939
display id: range2457939
version: 1
direction: direction/reverse
top level: dTomato
start: 44
end: 769
orientation: inline

BBa_J23110
type: SequenceAnnotation
persistent identity: annotation2457935
display id: annotation2457935
version: 1
top level: dTomato
location: https://synbiohub.org/public/igem/BBa_K1688011/annotation2457935/range2457935/1
component: BBa_J23110

https://synbiohub.org/public/igem/BBa_K1688011/annotation2457935/range2457935/1
type: Range
persistent identity: range2457935
display id: range2457935
version: 1
direction: direction/reverse
top level: dTomato
start: 1
end: 35
orientation: inline

ECFP
type: ComponentDefinition
persistent identity: BBa E0024
display id: BBa_E0024
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: enhanced cyan fluorescent protein derived from A. victoria GFP
creator: jcbraff
created: 2004-06-06T11:00:00Z
modified: 2015-08-31T04:07:25Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_1_
m user id: 0
owner id: 61
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence: https://synbiohub.org/public/igem/BBa_E0024_sequence/1

eYFP-GOI
type: ComponentDefinition
persistent identity: BBa K678010
display id: BBa_K678010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: eYFP-GOI
creator: DTU-Danmark-2
created: 2011-09-12T11:00:00Z
modified: 2015-05-08T01:13:04Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _882_
m user id: 0
owner id: 10556
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part was designed for the Plug'n'Play with DNA assembly standard (RFC 80), therefore this part contains tails at the 5' and 3' ends as shown on the figure below.
mutable notes: No
mutable provenance: The Danish Cancer Society
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: eYFP-GOI
sequence annotation: Kozak
sequence annotation: Linker 2
sequence: https://synbiohub.org/public/igem/BBa_K678010_sequence/1

eYFP-GOI
type: SequenceAnnotation
persistent identity: annotation2127765
display id: annotation2127765
version: 1
top level: eYFP-GOI
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K678010/annotation2127765/range2127765/1

https://synbiohub.org/public/igem/BBa_K678010/annotation2127765/range2127765/1
type: Range
persistent identity: range2127765
display id: range2127765
version: 1
direction: direction/forward
top level: eYFP-GOI
start: 16
end: 810
orientation: inline

Kozak
type: SequenceAnnotation
persistent identity: annotation2127776
display id: annotation2127776
version: 1
top level: eYFP-GOI
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678010/annotation2127776/range2127776/1

https://synbiohub.org/public/igem/BBa_K678010/annotation2127776/range2127776/1
type: Range
persistent identity: range2127776
display id: range2127776
version: 1
direction: direction/forward
top level: eYFP-GOI
start: 9
end: 19
orientation: inline

Linker 2
type: SequenceAnnotation
persistent identity: annotation2127764
display id: annotation2127764
version: 1
top level: eYFP-GOI
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678010/annotation2127764/range2127764/1

https://synbiohub.org/public/igem/BBa_K678010/annotation2127764/range2127764/1
type: Range
persistent identity: range2127764
display id: range2127764
version: 1
direction: direction/forward
top level: eYFP-GOI
start: 1
end: 8
orientation: inline

GFP1
type: ComponentDefinition
persistent identity: BBa I715019
display id: BBa_I715019
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Amino Half of GFP (aka GFP1)
creator: Will DeLoache
created: 2007-06-21T11:00:00Z
modified: 2015-08-31T04:07:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _120_
m user id: 0
owner id: 1491
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: A
mutable notes: A
mutable provenance: A
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: Coding Amino Portion GFP (aka GFP1)
sequence annotation: Start Codon
sequence: https://synbiohub.org/public/igem/BBa_I715019_sequence/1

Coding Amino Portion GFP (aka GFP1)
type: SequenceAnnotation
persistent identity: annotation1935357
display id: annotation1935357
version: 1
top level: GFP1
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_I715019/annotation1935357/range1935357/1

https://synbiohub.org/public/igem/BBa_I715019/annotation1935357/range1935357/1
type: Range
persistent identity: range1935357
display id: range1935357
version: 1
direction: direction/forward
top level: GFP1
start: 1
end: 471
orientation: inline

Start Codon
type: SequenceAnnotation
persistent identity: annotation1935356
display id: annotation1935356
version: 1
top level: GFP1
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_I715019/annotation1935356/range1935356/1

https://synbiohub.org/public/igem/BBa_I715019/annotation1935356/range1935356/1
type: Range
persistent identity: range1935356
display id: range1935356
version: 1
direction: direction/forward
top level: GFP1
start: 1
end: 3
orientation: inline

BBa_K1673410
type: ComponentDefinition
persistent identity: BBa K1673410
display id: BBa_K1673410
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: GFP (Mutation Optimized)
creator: Jarrod Shilts
created: 2015-09-17T11:00:00Z
modified: 2016-01-21T02:30:04Z
discontinued: false
dominant: false
group u list: _2091_
m user id: 4206
owner id: 19415
owning group id: 9
status: status/Planning
bookmark: false
mutable description: GFP, based on E0040, with overall mutation minimized
mutable notes: Sequence produced by the Vanderbilt iGEM 2015 mutation optimization software
mutable provenance: DNA Synthesis
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1673410_sequence/1

BBa_K389013
type: ComponentDefinition
persistent identity: BBa K389013
display id: BBa_K389013
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: VirA reporter system mRFP
creator: Jonas Aretz
created: 2010-09-10T11:00:00Z
modified: 2015-05-08T01:12:19Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _517_
m user id: 0
owner id: 6670
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: With this system it is possible to measure the activity of a virA receptor and the vir promoter, respectively. The reporter is the mRFP from the partsregistry.
mutable notes: medium strong constitutive promotor, terminator to keep basal expression of mRFP low
mutable provenance: partsregistry, synthetic gene by Mr. Gene, TI-plasmid from ''Agrobacterium tumefaciens'' C58
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_E1010
component: BBa_J23110
component: BBa_K389002
component: BBa_K389003
component: BBa_B0017
sequence annotation: BBa_E1010
sequence annotation: BBa_B0017
sequence annotation: BBa_K389002
sequence annotation: BBa_J23110
sequence annotation: BBa_K389003
sequence: https://synbiohub.org/public/igem/BBa_K389013_sequence/1

BBa_E1010
type: Component
persistent identity: component2263596
display id: component2263596
version: 1
top level: BBa_K389013
access: public
definition: mRFP1

BBa_J23110
type: Component
persistent identity: component2263579
display id: component2263579
version: 1
top level: BBa_K389013
access: public
definition: https://synbiohub.org/public/igem/BBa_J23110/1

BBa_K389002
type: Component
persistent identity: component2263583
display id: component2263583
version: 1
top level: BBa_K389013
access: public
definition: https://synbiohub.org/public/igem/BBa_K389002/1

BBa_K389003
type: Component
persistent identity: component2263593
display id: component2263593
version: 1
top level: BBa_K389013
access: public
definition: https://synbiohub.org/public/igem/BBa_K389003/1

BBa_B0017
type: Component
persistent identity: component2263588
display id: component2263588
version: 1
top level: BBa_K389013
access: public
definition: https://synbiohub.org/public/igem/BBa_B0017/1

BBa_E1010
type: SequenceAnnotation
persistent identity: annotation2263596
display id: annotation2263596
version: 1
top level: BBa_K389013
location: https://synbiohub.org/public/igem/BBa_K389013/annotation2263596/range2263596/1
component: BBa_E1010

https://synbiohub.org/public/igem/BBa_K389013/annotation2263596/range2263596/1
type: Range
persistent identity: range2263596
display id: range2263596
version: 1
direction: direction/reverse
top level: BBa_K389013
start: 1132
end: 1837
orientation: inline

BBa_B0017
type: SequenceAnnotation
persistent identity: annotation2263588
display id: annotation2263588
version: 1
top level: BBa_K389013
location: https://synbiohub.org/public/igem/BBa_K389013/annotation2263588/range2263588/1
component: BBa_B0017

https://synbiohub.org/public/igem/BBa_K389013/annotation2263588/range2263588/1
type: Range
persistent identity: range2263588
display id: range2263588
version: 1
direction: direction/reverse
top level: BBa_K389013
start: 792
end: 959
orientation: inline

BBa_K389002
type: SequenceAnnotation
persistent identity: annotation2263583
display id: annotation2263583
version: 1
top level: BBa_K389013
location: https://synbiohub.org/public/igem/BBa_K389013/annotation2263583/range2263583/1
component: BBa_K389002

https://synbiohub.org/public/igem/BBa_K389013/annotation2263583/range2263583/1
type: Range
persistent identity: range2263583
display id: range2263583
version: 1
direction: direction/reverse
top level: BBa_K389013
start: 44
end: 783
orientation: inline

BBa_J23110
type: SequenceAnnotation
persistent identity: annotation2263579
display id: annotation2263579
version: 1
top level: BBa_K389013
location: https://synbiohub.org/public/igem/BBa_K389013/annotation2263579/range2263579/1
component: BBa_J23110

https://synbiohub.org/public/igem/BBa_K389013/annotation2263579/range2263579/1
type: Range
persistent identity: range2263579
display id: range2263579
version: 1
direction: direction/reverse
top level: BBa_K389013
start: 1
end: 35
orientation: inline

BBa_K389003
type: SequenceAnnotation
persistent identity: annotation2263593
display id: annotation2263593
version: 1
top level: BBa_K389013
location: https://synbiohub.org/public/igem/BBa_K389013/annotation2263593/range2263593/1
component: BBa_K389003

https://synbiohub.org/public/igem/BBa_K389013/annotation2263593/range2263593/1
type: Range
persistent identity: range2263593
display id: range2263593
version: 1
direction: direction/reverse
top level: BBa_K389013
start: 968
end: 1125
orientation: inline

BBa_K1072000
type: ComponentDefinition
persistent identity: BBa K1072000
display id: BBa_K1072000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Lux pL controlled LuxR + lux pR autoinducing LuxI (lva tag) + lux pR controlled GFP.
creator: Yichong wang, Rui chen, Xihao liao and Junjie zhang
created: 2013-08-30T11:00:00Z
modified: 2015-05-08T01:09:01Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1381_
m user id: 0
owner id: 16002
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: BBa_K1072000 three protein generators: lux pl-RBS-C0062-B0015 produces LuxR. LuxR forms a complex with the quorum sensing molecule AHL and this complex subsequently increases the transcriptional rate of the lux pR promoter while also decreasing the transcriptional rate of the lux pL promoter. Both of the other generators are under control of this lux pR promoter. The second generator ???luxpR-RBS-J04031-B0015???has a low basal expression of the GFP, which can - of course - be monitored. The third generator produces luxI, which produces the quorum sensing molecule AHL. In combination with LuxR, AHL thus increases the production of AHL and GFP. This part is an improvement of BBa_K546000 and K546005 made by 2011 iGEM11_Wageningen_UR team. A LuxpR promoter, RBS, GFP and double terminator has been added.
mutable notes: constructing device compositing oscillator, coupling, reporter and productor.This part just is the oscillator and reporter.
mutable provenance: get from kit plate
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0010
component: BBa_R0062
component: BBa_B0012
component: BBa_E0040
component: BBa_K546000
component: BBa_B0034
sequence annotation: BBa_K546000
sequence annotation: BBa_E0040
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_R0062
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K1072000_sequence/1

BBa_B0010
type: Component
persistent identity: component2334214
display id: component2334214
version: 1
top level: BBa_K1072000
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_R0062
type: Component
persistent identity: component2334206
display id: component2334206
version: 1
top level: BBa_K1072000
access: public
definition: https://synbiohub.org/public/igem/BBa_R0062/1

BBa_B0012
type: Component
persistent identity: component2334216
display id: component2334216
version: 1
top level: BBa_K1072000
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_E0040
type: Component
persistent identity: component2334213
display id: component2334213
version: 1
top level: BBa_K1072000
access: public
definition: GFP

BBa_K546000
type: Component
persistent identity: component2334204
display id: component2334204
version: 1
top level: BBa_K1072000
access: public
definition: https://synbiohub.org/public/igem/BBa_K546000/1

BBa_B0034
type: Component
persistent identity: component2334211
display id: component2334211
version: 1
top level: BBa_K1072000
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K546000
type: SequenceAnnotation
persistent identity: annotation2334204
display id: annotation2334204
version: 1
top level: BBa_K1072000
location: https://synbiohub.org/public/igem/BBa_K1072000/annotation2334204/range2334204/1
component: BBa_K546000

https://synbiohub.org/public/igem/BBa_K1072000/annotation2334204/range2334204/1
type: Range
persistent identity: range2334204
display id: range2334204
version: 1
direction: direction/reverse
top level: BBa_K1072000
start: 1
end: 1964
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation2334213
display id: annotation2334213
version: 1
top level: BBa_K1072000
location: https://synbiohub.org/public/igem/BBa_K1072000/annotation2334213/range2334213/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_K1072000/annotation2334213/range2334213/1
type: Range
persistent identity: range2334213
display id: range2334213
version: 1
direction: direction/reverse
top level: BBa_K1072000
start: 2054
end: 2773
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2334214
display id: annotation2334214
version: 1
top level: BBa_K1072000
location: https://synbiohub.org/public/igem/BBa_K1072000/annotation2334214/range2334214/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K1072000/annotation2334214/range2334214/1
type: Range
persistent identity: range2334214
display id: range2334214
version: 1
direction: direction/forward
top level: BBa_K1072000
start: 2782
end: 2861
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2334216
display id: annotation2334216
version: 1
top level: BBa_K1072000
location: https://synbiohub.org/public/igem/BBa_K1072000/annotation2334216/range2334216/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K1072000/annotation2334216/range2334216/1
type: Range
persistent identity: range2334216
display id: range2334216
version: 1
direction: direction/forward
top level: BBa_K1072000
start: 2870
end: 2910
orientation: inline

BBa_R0062
type: SequenceAnnotation
persistent identity: annotation2334206
display id: annotation2334206
version: 1
top level: BBa_K1072000
location: https://synbiohub.org/public/igem/BBa_K1072000/annotation2334206/range2334206/1
component: BBa_R0062

https://synbiohub.org/public/igem/BBa_K1072000/annotation2334206/range2334206/1
type: Range
persistent identity: range2334206
display id: range2334206
version: 1
direction: direction/forward
top level: BBa_K1072000
start: 1973
end: 2027
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2334211
display id: annotation2334211
version: 1
top level: BBa_K1072000
location: https://synbiohub.org/public/igem/BBa_K1072000/annotation2334211/range2334211/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1072000/annotation2334211/range2334211/1
type: Range
persistent identity: range2334211
display id: range2334211
version: 1
direction: direction/reverse
top level: BBa_K1072000
start: 2036
end: 2047
orientation: inline

BBa_K157008
type: ComponentDefinition
persistent identity: BBa K157008
display id: BBa_K157008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Split-Venus-nYFP
creator: iGEM Team Freiburg 2008
created: 2008-10-25T11:00:00Z
modified: 2015-05-08T01:10:54Z
discontinued: false
dominant: false
experience: experience/None
group u list: _232_
m user id: 0
owner id: 1673
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: N-terminal fragment (Amino acids1-156) of the monomeric[5] yellow fluorescent protein variant ???Venus??? [4], designed for fusion/BiFC[1,3]. Reassembly with Part Bba_K157007 (Split-Venus-cYFP) generates the complete, working YFP ???Venus???, whereas combination with the C-terminal fragment (Part Bba_K157005) of the monomeric cyan fluorescent Protein ???CeruleanR206K??? (Part Bba_I757009) results in green fluorescence[2].See "Part Design" for references.
mutable notes: Between BioBrick 1.0 sites this part is flanked 5' by NgoMI(=NgoMIV) and 3' AgeI(=PinAI) to facilitate in frame cloning for protein fusions.
mutable provenance: Gene synthesis by GeneArt, optimized for expression in homo sapiens.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: N-terminal split venus YFP
sequence: https://synbiohub.org/public/igem/BBa_K157008_sequence/1

N-terminal split venus YFP
type: SequenceAnnotation
persistent identity: annotation2064750
display id: annotation2064750
version: 1
top level: BBa_K157008
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157008/annotation2064750/range2064750/1

https://synbiohub.org/public/igem/BBa_K157008/annotation2064750/range2064750/1
type: Range
persistent identity: range2064750
display id: range2064750
version: 1
direction: direction/forward
top level: BBa_K157008
start: 1
end: 468
orientation: inline

BBa_K533004
type: ComponentDefinition
persistent identity: BBa K533004
display id: BBa_K533004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: OmpA-mCherry
creator: ZHANG Yunxiao
created: 2011-09-28T11:00:00Z
modified: 2015-05-08T01:12:36Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _699_
m user id: 0
owner id: 6884
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This is designed as the positive control for protein presentation on the outer membrane.
mutable notes: His tag is inserted between OmpA and mCherry to facilitate detection after expression.
mutable provenance: OmpA is cloned from genomic DNA of BL21(DE3) E. Coli strain. mCherry is from the Clontech plasmid pmCherry-C1.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
sequence annotation: rbs
sequence annotation: T7
sequence annotation: OmpA
sequence annotation: stop
sequence annotation: mCherry
sequence annotation: lacI
sequence annotation: ATG
sequence annotation: His6Tag
sequence: https://synbiohub.org/public/igem/BBa_K533004_sequence/1

rbs
type: SequenceAnnotation
persistent identity: annotation2150607
display id: annotation2150607
version: 1
top level: BBa_K533004
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K533004/annotation2150607/range2150607/1

https://synbiohub.org/public/igem/BBa_K533004/annotation2150607/range2150607/1
type: Range
persistent identity: range2150607
display id: range2150607
version: 1
direction: direction/forward
top level: BBa_K533004
start: 75
end: 79
orientation: inline

T7
type: SequenceAnnotation
persistent identity: annotation2150605
display id: annotation2150605
version: 1
top level: BBa_K533004
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K533004/annotation2150605/range2150605/1

https://synbiohub.org/public/igem/BBa_K533004/annotation2150605/range2150605/1
type: Range
persistent identity: range2150605
display id: range2150605
version: 1
direction: direction/forward
top level: BBa_K533004
start: 1
end: 18
orientation: inline

OmpA
type: SequenceAnnotation
persistent identity: annotation2150609
display id: annotation2150609
version: 1
top level: BBa_K533004
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K533004/annotation2150609/range2150609/1

https://synbiohub.org/public/igem/BBa_K533004/annotation2150609/range2150609/1
type: Range
persistent identity: range2150609
display id: range2150609
version: 1
direction: direction/forward
top level: BBa_K533004
start: 87
end: 633
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2150612
display id: annotation2150612
version: 1
top level: BBa_K533004
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K533004/annotation2150612/range2150612/1

https://synbiohub.org/public/igem/BBa_K533004/annotation2150612/range2150612/1
type: Range
persistent identity: range2150612
display id: range2150612
version: 1
direction: direction/forward
top level: BBa_K533004
start: 1407
end: 1409
orientation: inline

mCherry
type: SequenceAnnotation
persistent identity: annotation2150611
display id: annotation2150611
version: 1
top level: BBa_K533004
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K533004/annotation2150611/range2150611/1

https://synbiohub.org/public/igem/BBa_K533004/annotation2150611/range2150611/1
type: Range
persistent identity: range2150611
display id: range2150611
version: 1
direction: direction/forward
top level: BBa_K533004
start: 699
end: 1409
orientation: inline

lacI
type: SequenceAnnotation
persistent identity: annotation2150606
display id: annotation2150606
version: 1
top level: BBa_K533004
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K533004/annotation2150606/range2150606/1

https://synbiohub.org/public/igem/BBa_K533004/annotation2150606/range2150606/1
type: Range
persistent identity: range2150606
display id: range2150606
version: 1
direction: direction/forward
top level: BBa_K533004
start: 20
end: 44
orientation: inline

ATG
type: SequenceAnnotation
persistent identity: annotation2150608
display id: annotation2150608
version: 1
top level: BBa_K533004
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K533004/annotation2150608/range2150608/1

https://synbiohub.org/public/igem/BBa_K533004/annotation2150608/range2150608/1
type: Range
persistent identity: range2150608
display id: range2150608
version: 1
direction: direction/forward
top level: BBa_K533004
start: 87
end: 89
orientation: inline

His6Tag
type: SequenceAnnotation
persistent identity: annotation2150610
display id: annotation2150610
version: 1
top level: BBa_K533004
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K533004/annotation2150610/range2150610/1

https://synbiohub.org/public/igem/BBa_K533004/annotation2150610/range2150610/1
type: Range
persistent identity: range2150610
display id: range2150610
version: 1
direction: direction/forward
top level: BBa_K533004
start: 648
end: 665
orientation: inline

BBa_K823039
type: ComponentDefinition
persistent identity: BBa K823039
display id: BBa_K823039
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: gfpmut1 (B. subtilis optimized + Freiburg standard)
creator: Tamara Cikovic
created: 2012-09-07T11:00:00Z
modified: 2015-05-08T01:13:30Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1081_
m user id: 0
owner id: 11555
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This gfpmut3 gene was amplified from pGFPamy B. subtilis vector. It is constructed in the Freiburg standard with RBS. prefix: GAATTCCGCGGCCGCTTCTAGATAAGGAGGAACTACTATGGCCGGC suffix: ACCGGTTAATACTAGTAGCGGCCGCTGCAGT
mutable notes: We designed this part in the Freiburg standard for N- and C-terminal fusions.
mutable provenance: Plasmid pGFPamy
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: gfpmut1
sequence: https://synbiohub.org/public/igem/BBa_K823039_sequence/1

gfpmut1
type: SequenceAnnotation
persistent identity: annotation2182335
display id: annotation2182335
version: 1
top level: BBa_K823039
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K823039/annotation2182335/range2182335/1

https://synbiohub.org/public/igem/BBa_K823039/annotation2182335/range2182335/1
type: Range
persistent identity: range2182335
display id: range2182335
version: 1
direction: direction/forward
top level: BBa_K823039
start: 1
end: 845
orientation: inline

BBa_K206003
type: ComponentDefinition
persistent identity: BBa K206003
display id: BBa_K206003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RFP generator for testing pBAD weak
creator: Amelia Hardjasa
created: 2009-10-17T11:00:00Z
modified: 2015-05-08T01:11:23Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _307_
m user id: 0
owner id: 4172
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This is a construct for testing promoter strength by measuring RFP fluorescence.
mutable notes: No special design considerations.
mutable provenance: Assembled from Registry BioBricks.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_I13507
component: BBa_K206001
sequence annotation: BBa_I13507
sequence annotation: BBa_K206001
sequence: https://synbiohub.org/public/igem/BBa_K206003_sequence/1

BBa_I13507
type: Component
persistent identity: component2256289
display id: component2256289
version: 1
top level: BBa_K206003
access: public
definition: https://synbiohub.org/public/igem/BBa_I13507/1

BBa_K206001
type: Component
persistent identity: component2256277
display id: component2256277
version: 1
top level: BBa_K206003
access: public
definition: https://synbiohub.org/public/igem/BBa_K206001/1

BBa_I13507
type: SequenceAnnotation
persistent identity: annotation2256289
display id: annotation2256289
version: 1
top level: BBa_K206003
location: https://synbiohub.org/public/igem/BBa_K206003/annotation2256289/range2256289/1
component: BBa_I13507

https://synbiohub.org/public/igem/BBa_K206003/annotation2256289/range2256289/1
type: Range
persistent identity: range2256289
display id: range2256289
version: 1
direction: direction/reverse
top level: BBa_K206003
start: 139
end: 999
orientation: inline

BBa_K206001
type: SequenceAnnotation
persistent identity: annotation2256277
display id: annotation2256277
version: 1
top level: BBa_K206003
location: https://synbiohub.org/public/igem/BBa_K206003/annotation2256277/range2256277/1
component: BBa_K206001

https://synbiohub.org/public/igem/BBa_K206003/annotation2256277/range2256277/1
type: Range
persistent identity: range2256277
display id: range2256277
version: 1
direction: direction/reverse
top level: BBa_K206003
start: 1
end: 130
orientation: inline

BFP
type: ComponentDefinition
persistent identity: BBa K592100
display id: BBa_K592100
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Blue Fluorescent Protein (mTagBFP)
creator: Erik Gullberg
created: 2011-09-19T11:00:00Z
modified: 2016-01-25T04:45:27Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _763_
m user id: 4206
owner id: 10137
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: This part codes for the bright blue fluorescent protein mTagBFP. mTagBFP is a monomeric protein with a narrow fluorescence emission spectrum with a maximum at 456 nm. It has a tyrosine-based chromophore, giving it substantially higher brightness, faster chromophore maturation and higher pH stability than blue fluorescent proteins with a histidine in the chromophore. Subach et. al. started with a red fluorescent protein (TagRed) and converted it to a bright blue monomeric protein. See the article listed in source. The sequence has been codon optimized for expression in E coli by DNA 2.0.
mutable notes: The sequence has been codon optimized for expression in E coli by DNA 2.0.
mutable provenance: Subach, O. M., I. S. Gundorov, et al. (2008). "Conversion of red fluorescent protein into a bright blue probe." Chem Biol 15(10): 1116-24.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: mTagBFP
sequence: https://synbiohub.org/public/igem/BBa_K592100_sequence/1

mTagBFP
type: SequenceAnnotation
persistent identity: annotation2135490
display id: annotation2135490
version: 1
top level: BFP
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K592100/annotation2135490/range2135490/1

https://synbiohub.org/public/igem/BBa_K592100/annotation2135490/range2135490/1
type: Range
persistent identity: range2135490
display id: range2135490
version: 1
direction: direction/forward
top level: BFP
start: 1
end: 699
orientation: inline

BBa_K1399006
type: ComponentDefinition
persistent identity: BBa K1399006
display id: BBa_K1399006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: GFP (mut3b) with LAA-ssrA degradation tag
creator: Anna Stikane
created: 2014-09-18T11:00:00Z
modified: 2015-05-08T01:10:16Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1777_
m user id: 0
owner id: 22477
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: GFP (mut3b) (see part BBa_E0040) with added LAA-ssrA degradation tag (see part BBa_M0050). The tag increases GFP turn-over rate, thus providing better temporal resolution of green fluorescence. In the same time, maximal fluorescence amplitudes will be lower as newly formed protein is degraded as soon as it is formed. The tag encodes E.coli wild type ssrA peptide sequence AANDENYALAA and is recognized by ClpA and ClpX unfoldases and ClpX mediator SspB.[1] ClpA and ClpX then form a proteosome-like complex with ClpP protease and the protein is degraded.[1] The final three residues of the tag determines the strength of interaction with ClpX and thus the final protein degradation rate.[2] The LAA tag is reported to lead to fast protein degradation, degrading GFP with rate -0.018 per minute.[2] However, be aware that exact protein degradation rate depends on multiple factors: ClpXP and ClpAP protease and SspB mediator concentrations, protein stability, Km of binding to the protease, temperature [3]. ===References=== [1] Flynn, J. M. et al. Overlapping recognition determinants within the ssrA degradation tag allow modulation of proteolysis. Proc. Natl. Acad. Sci. U. S. A. 98, 10584???9 (2001). [2] Andersen, J. B. et al. New unstable variants of green fluorescent protein for studies of transient gene expression in bacteria. Appl. Environ. Microbiol. 64, 2240???6 (1998). [3] Purcell, O., Grierson, C. S., Bernardo, M. Di & Savery, N. J. Temperature dependence of ssrA-tag mediated protein degradation. J. Biol. Eng. 6, 10 (2012).
mutable notes: The tag was attached to GFP using PCR and MABEL (mutagenesis with blunt-end ligation), thus avoiding introduction of additional residues and restriction site. Different parts of the tag are recognized by different proteins, for example, the final 3 residues (LAA in this case) are recognized by ClpX, whereas first 4 residues of the tag are required for efficient SspB binding.[1] Thus modifications of these critical residues alter the efficacy with what different proteases bind to it.
mutable provenance: GFP comes from part BBa_E1010, the tag sequence was obtained from part BBa_M0050.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: stop
sequence annotation: start
sequence annotation: LAA-ssrA
sequence annotation: GFP (mut3b)
sequence: https://synbiohub.org/public/igem/BBa_K1399006_sequence/1

stop
type: SequenceAnnotation
persistent identity: annotation2383921
display id: annotation2383921
version: 1
top level: BBa_K1399006
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1399006/annotation2383921/range2383921/1

https://synbiohub.org/public/igem/BBa_K1399006/annotation2383921/range2383921/1
type: Range
persistent identity: range2383921
display id: range2383921
version: 1
direction: direction/forward
top level: BBa_K1399006
start: 748
end: 750
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2383918
display id: annotation2383918
version: 1
top level: BBa_K1399006
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1399006/annotation2383918/range2383918/1

https://synbiohub.org/public/igem/BBa_K1399006/annotation2383918/range2383918/1
type: Range
persistent identity: range2383918
display id: range2383918
version: 1
direction: direction/forward
top level: BBa_K1399006
start: 1
end: 3
orientation: inline

LAA-ssrA
type: SequenceAnnotation
persistent identity: annotation2383920
display id: annotation2383920
version: 1
top level: BBa_K1399006
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1399006/annotation2383920/range2383920/1

https://synbiohub.org/public/igem/BBa_K1399006/annotation2383920/range2383920/1
type: Range
persistent identity: range2383920
display id: range2383920
version: 1
direction: direction/forward
top level: BBa_K1399006
start: 715
end: 747
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2383922
display id: annotation2383922
version: 1
top level: BBa_K1399006
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1399006/annotation2383922/range2383922/1

https://synbiohub.org/public/igem/BBa_K1399006/annotation2383922/range2383922/1
type: Range
persistent identity: range2383922
display id: range2383922
version: 1
direction: direction/forward
top level: BBa_K1399006
start: 751
end: 753
orientation: inline

GFP (mut3b)
type: SequenceAnnotation
persistent identity: annotation2383919
display id: annotation2383919
version: 1
top level: BBa_K1399006
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1399006/annotation2383919/range2383919/1

https://synbiohub.org/public/igem/BBa_K1399006/annotation2383919/range2383919/1
type: Range
persistent identity: range2383919
display id: range2383919
version: 1
direction: direction/forward
top level: BBa_K1399006
start: 4
end: 714
orientation: inline

sfBFPN
type: ComponentDefinition
persistent identity: BBa K1093003
display id: BBa_K1093003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: sfBFP N-terminal
creator: Anna Miścicka
created: 2013-09-19T11:00:00Z
modified: 2016-01-26T03:06:25Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1403_
m user id: 4206
owner id: 18431
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: Superfolder Blue Fluorescent Protein is a derivative of superfolder Green Fluorescent protein. Compared to eBFP it displays improved stability and strong blue fluorescence. Excitation peak: 380 nm Emission peak: 440 nm. This is N-terminal of sfBFP, that can be used in Bimolecular Fluorescence Complementation system (BiFC).
mutable notes: Created by PCR on sfBFP.
mutable provenance: PartsRegistry BBa_I746909, originally from Aequorea victoria.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: start
sequence annotation: sfBFP N-terminal
sequence: https://synbiohub.org/public/igem/BBa_K1093003_sequence/1

start
type: SequenceAnnotation
persistent identity: annotation2357234
display id: annotation2357234
version: 1
top level: sfBFPN
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1093003/annotation2357234/range2357234/1

https://synbiohub.org/public/igem/BBa_K1093003/annotation2357234/range2357234/1
type: Range
persistent identity: range2357234
display id: range2357234
version: 1
direction: direction/forward
top level: sfBFPN
start: 1
end: 3
orientation: inline

sfBFP N-terminal
type: SequenceAnnotation
persistent identity: annotation2357235
display id: annotation2357235
version: 1
top level: sfBFPN
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1093003/annotation2357235/range2357235/1

https://synbiohub.org/public/igem/BBa_K1093003/annotation2357235/range2357235/1
type: Range
persistent identity: range2357235
display id: range2357235
version: 1
direction: direction/forward
top level: sfBFPN
start: 1
end: 645
orientation: inline

BBa_K863122
type: ComponentDefinition
persistent identity: BBa K863122
display id: BBa_K863122
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: BBa_I13522 with His-tag
creator: Moritz M??ller
created: 2012-09-19T11:00:00Z
modified: 2015-05-08T01:13:36Z
discontinued: false
dominant: false
group u list: _1123_
m user id: 0
owner id: 13080
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: [http://partsregistry.org/Part:BBa_I13522 BBa_I13522] with His-tag and without the terminators
mutable notes: [http://partsregistry.org/Part:BBa_I13522 BBa_I13522] with His-tag and without the terminators
mutable provenance: [http://partsregistry.org/Part:BBa_I13522 BBa_I13522] with His-tag and without the terminators
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: BBa_I13522
sequence annotation: -35
sequence annotation: AgeI
sequence annotation: TetR 2
sequence annotation: conserved
sequence annotation: BBa_E0040
sequence annotation: BBa_B0034
sequence annotation: -10
sequence annotation: TetR 1
sequence annotation: stop
sequence annotation: His-tag
sequence annotation: BBa_R0040
sequence: https://synbiohub.org/public/igem/BBa_K863122_sequence/1

BBa_I13522
type: SequenceAnnotation
persistent identity: annotation2188231
display id: annotation2188231
version: 1
top level: BBa_K863122
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K863122/annotation2188231/range2188231/1

https://synbiohub.org/public/igem/BBa_K863122/annotation2188231/range2188231/1
type: Range
persistent identity: range2188231
display id: range2188231
version: 1
direction: direction/reverse
top level: BBa_K863122
start: 1
end: 800
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2188232
display id: annotation2188232
version: 1
top level: BBa_K863122
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K863122/annotation2188232/range2188232/1

https://synbiohub.org/public/igem/BBa_K863122/annotation2188232/range2188232/1
type: Range
persistent identity: range2188232
display id: range2188232
version: 1
direction: direction/forward
top level: BBa_K863122
start: 20
end: 25
orientation: inline

AgeI
type: SequenceAnnotation
persistent identity: annotation2188257
display id: annotation2188257
version: 1
top level: BBa_K863122
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K863122/annotation2188257/range2188257/1

https://synbiohub.org/public/igem/BBa_K863122/annotation2188257/range2188257/1
type: Range
persistent identity: range2188257
display id: range2188257
version: 1
direction: direction/forward
top level: BBa_K863122
start: 813
end: 818
orientation: inline

TetR 2
type: SequenceAnnotation
persistent identity: annotation2188233
display id: annotation2188233
version: 1
top level: BBa_K863122
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K863122/annotation2188233/range2188233/1

https://synbiohub.org/public/igem/BBa_K863122/annotation2188233/range2188233/1
type: Range
persistent identity: range2188233
display id: range2188233
version: 1
direction: direction/forward
top level: BBa_K863122
start: 26
end: 44
orientation: inline

conserved
type: SequenceAnnotation
persistent identity: annotation2188236
display id: annotation2188236
version: 1
top level: BBa_K863122
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K863122/annotation2188236/range2188236/1

https://synbiohub.org/public/igem/BBa_K863122/annotation2188236/range2188236/1
type: Range
persistent identity: range2188236
display id: range2188236
version: 1
direction: direction/forward
top level: BBa_K863122
start: 67
end: 70
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation2188237
display id: annotation2188237
version: 1
top level: BBa_K863122
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K863122/annotation2188237/range2188237/1

https://synbiohub.org/public/igem/BBa_K863122/annotation2188237/range2188237/1
type: Range
persistent identity: range2188237
display id: range2188237
version: 1
direction: direction/forward
top level: BBa_K863122
start: 81
end: 800
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2188235
display id: annotation2188235
version: 1
top level: BBa_K863122
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K863122/annotation2188235/range2188235/1

https://synbiohub.org/public/igem/BBa_K863122/annotation2188235/range2188235/1
type: Range
persistent identity: range2188235
display id: range2188235
version: 1
direction: direction/forward
top level: BBa_K863122
start: 63
end: 74
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2188234
display id: annotation2188234
version: 1
top level: BBa_K863122
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K863122/annotation2188234/range2188234/1

https://synbiohub.org/public/igem/BBa_K863122/annotation2188234/range2188234/1
type: Range
persistent identity: range2188234
display id: range2188234
version: 1
direction: direction/forward
top level: BBa_K863122
start: 43
end: 48
orientation: inline

TetR 1
type: SequenceAnnotation
persistent identity: annotation2188230
display id: annotation2188230
version: 1
top level: BBa_K863122
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K863122/annotation2188230/range2188230/1

https://synbiohub.org/public/igem/BBa_K863122/annotation2188230/range2188230/1
type: Range
persistent identity: range2188230
display id: range2188230
version: 1
direction: direction/forward
top level: BBa_K863122
start: 1
end: 19
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2188238
display id: annotation2188238
version: 1
top level: BBa_K863122
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K863122/annotation2188238/range2188238/1

https://synbiohub.org/public/igem/BBa_K863122/annotation2188238/range2188238/1
type: Range
persistent identity: range2188238
display id: range2188238
version: 1
direction: direction/forward
top level: BBa_K863122
start: 819
end: 821
orientation: inline

His-tag
type: SequenceAnnotation
persistent identity: annotation2188253
display id: annotation2188253
version: 1
top level: BBa_K863122
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K863122/annotation2188253/range2188253/1

https://synbiohub.org/public/igem/BBa_K863122/annotation2188253/range2188253/1
type: Range
persistent identity: range2188253
display id: range2188253
version: 1
direction: direction/forward
top level: BBa_K863122
start: 801
end: 812
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2188229
display id: annotation2188229
version: 1
top level: BBa_K863122
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K863122/annotation2188229/range2188229/1

https://synbiohub.org/public/igem/BBa_K863122/annotation2188229/range2188229/1
type: Range
persistent identity: range2188229
display id: range2188229
version: 1
direction: direction/forward
top level: BBa_K863122
start: 1
end: 54
orientation: inline

BBa_K1899003
type: ComponentDefinition
persistent identity: BBa K1899003
display id: BBa_K1899003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Superfolder GFP
creator: iGEM2016_HKUST
created: 2016-08-27T11:00:00Z
modified: 2016-10-15T04:03:09Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2365_
m user id: 26069
owner id: 26069
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Variant of GFP that folds robustly even when fused to poorly folded proteins. Waldo and other research groups has reported the engineering of a superfolder GFP (sfGFP) that showed increased resistance to denaturation, improved folding kinetics, and increased resistance to aggregation during refolding (Pedelacq et al., 2006; Andrews et al., 2007; Fisher and DeLisa, 2008). sfGFP has proven to be very useful as a scaffold for improved protein detection and tagging both in vivo and in vitro using self-assembled sfGFP fragments (Cabantous et al., 2005b; Cabantous and Waldo, 2006). Furthermore, sfGFP fusions are more soluble than conventional GFP fusions (Wu et al., 2009).
mutable notes: nil
mutable provenance: Waldo lab
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: scar
sequence annotation: BBa_J61048
sequence annotation: sfGFP
sequence annotation: BBa_B0032
sequence: https://synbiohub.org/public/igem/BBa_K1899003_sequence/1

scar
type: SequenceAnnotation
persistent identity: annotation2481804
display id: annotation2481804
version: 1
top level: BBa_K1899003
role: feature/scar
location: https://synbiohub.org/public/igem/BBa_K1899003/annotation2481804/range2481804/1

https://synbiohub.org/public/igem/BBa_K1899003/annotation2481804/range2481804/1
type: Range
persistent identity: range2481804
display id: range2481804
version: 1
direction: direction/forward
top level: BBa_K1899003
start: 13
end: 19
orientation: inline

BBa_J61048
type: SequenceAnnotation
persistent identity: annotation2481805
display id: annotation2481805
version: 1
top level: BBa_K1899003
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1899003/annotation2481805/range2481805/1

https://synbiohub.org/public/igem/BBa_K1899003/annotation2481805/range2481805/1
type: Range
persistent identity: range2481805
display id: range2481805
version: 1
direction: direction/forward
top level: BBa_K1899003
start: 742
end: 854
orientation: inline

sfGFP
type: SequenceAnnotation
persistent identity: annotation2481803
display id: annotation2481803
version: 1
top level: BBa_K1899003
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1899003/annotation2481803/range2481803/1

https://synbiohub.org/public/igem/BBa_K1899003/annotation2481803/range2481803/1
type: Range
persistent identity: range2481803
display id: range2481803
version: 1
direction: direction/forward
top level: BBa_K1899003
start: 20
end: 733
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation2481802
display id: annotation2481802
version: 1
top level: BBa_K1899003
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1899003/annotation2481802/range2481802/1

https://synbiohub.org/public/igem/BBa_K1899003/annotation2481802/range2481802/1
type: Range
persistent identity: range2481802
display id: range2481802
version: 1
direction: direction/forward
top level: BBa_K1899003
start: 1
end: 12
orientation: inline

scar
type: SequenceAnnotation
persistent identity: annotation2481806
display id: annotation2481806
version: 1
top level: BBa_K1899003
role: feature/scar
location: https://synbiohub.org/public/igem/BBa_K1899003/annotation2481806/range2481806/1

https://synbiohub.org/public/igem/BBa_K1899003/annotation2481806/range2481806/1
type: Range
persistent identity: range2481806
display id: range2481806
version: 1
direction: direction/forward
top level: BBa_K1899003
start: 734
end: 741
orientation: inline

Tm-Fluolin
type: ComponentDefinition
persistent identity: BBa K157016
display id: BBa_K157016
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Tm-Fluolinker-cCFP
creator: iGEM Team Freiburg 2008
created: 2008-10-25T11:00:00Z
modified: 2015-05-08T01:10:54Z
discontinued: false
dominant: false
experience: experience/None
group u list: _232_
m user id: 0
owner id: 1675
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: C-terminal part of the Split-fluorophore CFP, fused to the trans-membrane region of the Erbb1/EGF-receptor with the fluo-linker in between.
mutable notes: none
mutable provenance: Derived by cloning of the parts BBa_K157002, BBa_K157009 and BBa_K157005. For further information see the description of the parts mentioned above.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: Fluolinker
sequence annotation: C-Term_CFP
sequence annotation: Tm
sequence annotation: AgeI
sequence annotation: NgoMI
sequence annotation: Scar (Thr, Gly)
sequence annotation: Tm-Fluolinker-cCFP
sequence annotation: Sca (Thr, Gly)
sequence: https://synbiohub.org/public/igem/BBa_K157016_sequence/1

Fluolinker
type: SequenceAnnotation
persistent identity: annotation1991282
display id: annotation1991282
version: 1
top level: Tm-Fluolin
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157016/annotation1991282/range1991282/1

https://synbiohub.org/public/igem/BBa_K157016/annotation1991282/range1991282/1
type: Range
persistent identity: range1991282
display id: range1991282
version: 1
direction: direction/forward
top level: Tm-Fluolin
start: 85
end: 135
orientation: inline

C-Term_CFP
type: SequenceAnnotation
persistent identity: annotation1991284
display id: annotation1991284
version: 1
top level: Tm-Fluolin
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157016/annotation1991284/range1991284/1

https://synbiohub.org/public/igem/BBa_K157016/annotation1991284/range1991284/1
type: Range
persistent identity: range1991284
display id: range1991284
version: 1
direction: direction/forward
top level: Tm-Fluolin
start: 142
end: 384
orientation: inline

Tm
type: SequenceAnnotation
persistent identity: annotation1991280
display id: annotation1991280
version: 1
top level: Tm-Fluolin
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157016/annotation1991280/range1991280/1

https://synbiohub.org/public/igem/BBa_K157016/annotation1991280/range1991280/1
type: Range
persistent identity: range1991280
display id: range1991280
version: 1
direction: direction/forward
top level: Tm-Fluolin
start: 10
end: 78
orientation: inline

AgeI
type: SequenceAnnotation
persistent identity: annotation1989970
display id: annotation1989970
version: 1
top level: Tm-Fluolin
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157016/annotation1989970/range1989970/1

https://synbiohub.org/public/igem/BBa_K157016/annotation1989970/range1989970/1
type: Range
persistent identity: range1989970
display id: range1989970
version: 1
direction: direction/forward
top level: Tm-Fluolin
start: 385
end: 390
orientation: inline

NgoMI
type: SequenceAnnotation
persistent identity: annotation1989969
display id: annotation1989969
version: 1
top level: Tm-Fluolin
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157016/annotation1989969/range1989969/1

https://synbiohub.org/public/igem/BBa_K157016/annotation1989969/range1989969/1
type: Range
persistent identity: range1989969
display id: range1989969
version: 1
direction: direction/forward
top level: Tm-Fluolin
start: 4
end: 9
orientation: inline

Scar (Thr, Gly)
type: SequenceAnnotation
persistent identity: annotation1991281
display id: annotation1991281
version: 1
top level: Tm-Fluolin
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157016/annotation1991281/range1991281/1

https://synbiohub.org/public/igem/BBa_K157016/annotation1991281/range1991281/1
type: Range
persistent identity: range1991281
display id: range1991281
version: 1
direction: direction/forward
top level: Tm-Fluolin
start: 79
end: 84
orientation: inline

Tm-Fluolinker-cCFP
type: SequenceAnnotation
persistent identity: annotation1989971
display id: annotation1989971
version: 1
top level: Tm-Fluolin
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K157016/annotation1989971/range1989971/1

https://synbiohub.org/public/igem/BBa_K157016/annotation1989971/range1989971/1
type: Range
persistent identity: range1989971
display id: range1989971
version: 1
direction: direction/forward
top level: Tm-Fluolin
start: 1
end: 390
orientation: inline

Sca (Thr, Gly)
type: SequenceAnnotation
persistent identity: annotation1991283
display id: annotation1991283
version: 1
top level: Tm-Fluolin
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157016/annotation1991283/range1991283/1

https://synbiohub.org/public/igem/BBa_K157016/annotation1991283/range1991283/1
type: Range
persistent identity: range1991283
display id: range1991283
version: 1
direction: direction/forward
top level: Tm-Fluolin
start: 136
end: 141
orientation: inline

eyfp
type: ComponentDefinition
persistent identity: BBa E0030
display id: BBa_E0030
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: enhanced yellow fluorescent protein derived from A. victoria GFP
creator: Caitlin Conboy and Jennifer Braff
created: 2004-03-02T12:00:00Z
modified: 2015-08-31T04:07:25Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: -- No description --
mutable provenance: Modificaitons to Clontech EYFP by Reshma Shetty
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_E0030_sequence/1

BBa_K1399012
type: ComponentDefinition
persistent identity: BBa K1399012
display id: BBa_K1399012
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Plac-RFP(LAA)
creator: Anna Stikane
created: 2014-09-18T11:00:00Z
modified: 2015-05-08T01:10:16Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1777_
m user id: 0
owner id: 22477
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: Lactose/IPTG inducible promoter with RBS, RFP reporter tagged with LAA-ssrA degradation tag and terminator. The tagged RFP is actively degraded within cell, thus provides better temporal resolution of red fluorescence and promoter activity.
mutable notes: Part was assembled using BrickClip assembly (BBF RFC 104) using other biobrick parts as templates. BrickClip assembly is a special case of more general Paperclip assembly method.[1]
mutable provenance: other biobricks
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: stop
sequence annotation: BBa_R0010
sequence annotation: BBa_B0034
sequence annotation: end of LacI coding region (inactive)
sequence annotation: BBa_B0012
sequence annotation: RFP
sequence annotation: -10
sequence annotation: BBa_B0015
sequence annotation: barcode from BBa_E1010
sequence annotation: CAP binding site
sequence annotation: start
sequence annotation: -35
sequence annotation: T7 TE
sequence annotation: stem_loop
sequence annotation: BBa_B0010
sequence annotation: BBa_K1399002
sequence annotation: polya
sequence annotation: conserved
sequence annotation: tag
sequence annotation: LacI binding site
sequence: https://synbiohub.org/public/igem/BBa_K1399012_sequence/1

stop
type: SequenceAnnotation
persistent identity: annotation2383998
display id: annotation2383998
version: 1
top level: BBa_K1399012
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1399012/annotation2383998/range2383998/1

https://synbiohub.org/public/igem/BBa_K1399012/annotation2383998/range2383998/1
type: Range
persistent identity: range2383998
display id: range2383998
version: 1
direction: direction/forward
top level: BBa_K1399012
start: 935
end: 937
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation2383986
display id: annotation2383986
version: 1
top level: BBa_K1399012
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1399012/annotation2383986/range2383986/1

https://synbiohub.org/public/igem/BBa_K1399012/annotation2383986/range2383986/1
type: Range
persistent identity: range2383986
display id: range2383986
version: 1
direction: direction/forward
top level: BBa_K1399012
start: 1
end: 200
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2383994
display id: annotation2383994
version: 1
top level: BBa_K1399012
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1399012/annotation2383994/range2383994/1

https://synbiohub.org/public/igem/BBa_K1399012/annotation2383994/range2383994/1
type: Range
persistent identity: range2383994
display id: range2383994
version: 1
direction: direction/reverse
top level: BBa_K1399012
start: 209
end: 220
orientation: inline

end of LacI coding region (inactive)
type: SequenceAnnotation
persistent identity: annotation2383987
display id: annotation2383987
version: 1
top level: BBa_K1399012
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1399012/annotation2383987/range2383987/1

https://synbiohub.org/public/igem/BBa_K1399012/annotation2383987/range2383987/1
type: Range
persistent identity: range2383987
display id: range2383987
version: 1
direction: direction/forward
top level: BBa_K1399012
start: 1
end: 88
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2384003
display id: annotation2384003
version: 1
top level: BBa_K1399012
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1399012/annotation2384003/range2384003/1

https://synbiohub.org/public/igem/BBa_K1399012/annotation2384003/range2384003/1
type: Range
persistent identity: range2384003
display id: range2384003
version: 1
direction: direction/forward
top level: BBa_K1399012
start: 1062
end: 1102
orientation: inline

RFP
type: SequenceAnnotation
persistent identity: annotation2383996
display id: annotation2383996
version: 1
top level: BBa_K1399012
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1399012/annotation2383996/range2383996/1

https://synbiohub.org/public/igem/BBa_K1399012/annotation2383996/range2383996/1
type: Range
persistent identity: range2383996
display id: range2383996
version: 1
direction: direction/forward
top level: BBa_K1399012
start: 230
end: 901
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2383990
display id: annotation2383990
version: 1
top level: BBa_K1399012
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1399012/annotation2383990/range2383990/1

https://synbiohub.org/public/igem/BBa_K1399012/annotation2383990/range2383990/1
type: Range
persistent identity: range2383990
display id: range2383990
version: 1
direction: direction/forward
top level: BBa_K1399012
start: 161
end: 166
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2384006
display id: annotation2384006
version: 1
top level: BBa_K1399012
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1399012/annotation2384006/range2384006/1

https://synbiohub.org/public/igem/BBa_K1399012/annotation2384006/range2384006/1
type: Range
persistent identity: range2384006
display id: range2384006
version: 1
direction: direction/forward
top level: BBa_K1399012
start: 1095
end: 1095
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2384007
display id: annotation2384007
version: 1
top level: BBa_K1399012
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1399012/annotation2384007/range2384007/1

https://synbiohub.org/public/igem/BBa_K1399012/annotation2384007/range2384007/1
type: Range
persistent identity: range2384007
display id: range2384007
version: 1
direction: direction/reverse
top level: BBa_K1399012
start: 974
end: 1102
orientation: inline

barcode from BBa_E1010
type: SequenceAnnotation
persistent identity: annotation2386312
display id: annotation2386312
version: 1
top level: BBa_K1399012
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1399012/annotation2386312/range2386312/1

https://synbiohub.org/public/igem/BBa_K1399012/annotation2386312/range2386312/1
type: Range
persistent identity: range2386312
display id: range2386312
version: 1
direction: direction/forward
top level: BBa_K1399012
start: 941
end: 965
orientation: inline

CAP binding site
type: SequenceAnnotation
persistent identity: annotation2383988
display id: annotation2383988
version: 1
top level: BBa_K1399012
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1399012/annotation2383988/range2383988/1

https://synbiohub.org/public/igem/BBa_K1399012/annotation2383988/range2383988/1
type: Range
persistent identity: range2383988
display id: range2383988
version: 1
direction: direction/forward
top level: BBa_K1399012
start: 89
end: 126
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2383995
display id: annotation2383995
version: 1
top level: BBa_K1399012
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1399012/annotation2383995/range2383995/1

https://synbiohub.org/public/igem/BBa_K1399012/annotation2383995/range2383995/1
type: Range
persistent identity: range2383995
display id: range2383995
version: 1
direction: direction/forward
top level: BBa_K1399012
start: 227
end: 229
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2383992
display id: annotation2383992
version: 1
top level: BBa_K1399012
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1399012/annotation2383992/range2383992/1

https://synbiohub.org/public/igem/BBa_K1399012/annotation2383992/range2383992/1
type: Range
persistent identity: range2383992
display id: range2383992
version: 1
direction: direction/forward
top level: BBa_K1399012
start: 173
end: 173
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2383989
display id: annotation2383989
version: 1
top level: BBa_K1399012
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1399012/annotation2383989/range2383989/1

https://synbiohub.org/public/igem/BBa_K1399012/annotation2383989/range2383989/1
type: Range
persistent identity: range2383989
display id: range2383989
version: 1
direction: direction/forward
top level: BBa_K1399012
start: 137
end: 142
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation2384004
display id: annotation2384004
version: 1
top level: BBa_K1399012
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1399012/annotation2384004/range2384004/1

https://synbiohub.org/public/igem/BBa_K1399012/annotation2384004/range2384004/1
type: Range
persistent identity: range2384004
display id: range2384004
version: 1
direction: direction/forward
top level: BBa_K1399012
start: 1069
end: 1088
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation2384002
display id: annotation2384002
version: 1
top level: BBa_K1399012
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1399012/annotation2384002/range2384002/1

https://synbiohub.org/public/igem/BBa_K1399012/annotation2384002/range2384002/1
type: Range
persistent identity: range2384002
display id: range2384002
version: 1
direction: direction/forward
top level: BBa_K1399012
start: 985
end: 1028
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2384001
display id: annotation2384001
version: 1
top level: BBa_K1399012
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1399012/annotation2384001/range2384001/1

https://synbiohub.org/public/igem/BBa_K1399012/annotation2384001/range2384001/1
type: Range
persistent identity: range2384001
display id: range2384001
version: 1
direction: direction/forward
top level: BBa_K1399012
start: 974
end: 1053
orientation: inline

BBa_K1399002
type: SequenceAnnotation
persistent identity: annotation2384000
display id: annotation2384000
version: 1
top level: BBa_K1399012
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1399012/annotation2384000/range2384000/1

https://synbiohub.org/public/igem/BBa_K1399012/annotation2384000/range2384000/1
type: Range
persistent identity: range2384000
display id: range2384000
version: 1
direction: direction/reverse
top level: BBa_K1399012
start: 227
end: 940
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation2384005
display id: annotation2384005
version: 1
top level: BBa_K1399012
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_K1399012/annotation2384005/range2384005/1

https://synbiohub.org/public/igem/BBa_K1399012/annotation2384005/range2384005/1
type: Range
persistent identity: range2384005
display id: range2384005
version: 1
direction: direction/forward
top level: BBa_K1399012
start: 1089
end: 1102
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2383999
display id: annotation2383999
version: 1
top level: BBa_K1399012
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1399012/annotation2383999/range2383999/1

https://synbiohub.org/public/igem/BBa_K1399012/annotation2383999/range2383999/1
type: Range
persistent identity: range2383999
display id: range2383999
version: 1
direction: direction/forward
top level: BBa_K1399012
start: 938
end: 940
orientation: inline

conserved
type: SequenceAnnotation
persistent identity: annotation2383993
display id: annotation2383993
version: 1
top level: BBa_K1399012
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K1399012/annotation2383993/range2383993/1

https://synbiohub.org/public/igem/BBa_K1399012/annotation2383993/range2383993/1
type: Range
persistent identity: range2383993
display id: range2383993
version: 1
direction: direction/forward
top level: BBa_K1399012
start: 213
end: 216
orientation: inline

tag
type: SequenceAnnotation
persistent identity: annotation2383997
display id: annotation2383997
version: 1
top level: BBa_K1399012
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1399012/annotation2383997/range2383997/1

https://synbiohub.org/public/igem/BBa_K1399012/annotation2383997/range2383997/1
type: Range
persistent identity: range2383997
display id: range2383997
version: 1
direction: direction/forward
top level: BBa_K1399012
start: 902
end: 934
orientation: inline

LacI binding site
type: SequenceAnnotation
persistent identity: annotation2383991
display id: annotation2383991
version: 1
top level: BBa_K1399012
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1399012/annotation2383991/range2383991/1

https://synbiohub.org/public/igem/BBa_K1399012/annotation2383991/range2383991/1
type: Range
persistent identity: range2383991
display id: range2383991
version: 1
direction: direction/forward
top level: BBa_K1399012
start: 166
end: 200
orientation: inline

BBa_I15014
type: ComponentDefinition
persistent identity: BBa I15014
display id: BBa_I15014
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: polycistronic EYFP+ECFP
creator: Jeff Tabor
created: 2005-05-02T11:00:00Z
modified: 2015-08-31T04:07:38Z
discontinued: false
dominant: true
experience: experience/None
group u list: _5_
m user id: 0
owner id: 88
owning group id: 5
sample status: In stock
status: status/Available
bookmark: false
mutable description: This part accepts POPS to produce a single mRNA containing EYFP and ECFP under the strong clonetech RBS
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_E0031
component: BBa_E0021
sequence annotation: BBa_E0021
sequence annotation: BBa_E0031
sequence: https://synbiohub.org/public/igem/BBa_I15014_sequence/1

BBa_E0031
type: Component
persistent identity: component1482868
display id: component1482868
version: 1
top level: BBa_I15014
access: public
definition: eyfp

BBa_E0021
type: Component
persistent identity: component1482879
display id: component1482879
version: 1
top level: BBa_I15014
access: public
definition: ecfp

BBa_E0021
type: SequenceAnnotation
persistent identity: annotation1482879
display id: annotation1482879
version: 1
top level: BBa_I15014
location: https://synbiohub.org/public/igem/BBa_I15014/annotation1482879/range1482879/1
component: BBa_E0021

https://synbiohub.org/public/igem/BBa_I15014/annotation1482879/range1482879/1
type: Range
persistent identity: range1482879
display id: range1482879
version: 1
direction: direction/forward
top level: BBa_I15014
start: 749
end: 1488
orientation: inline

BBa_E0031
type: SequenceAnnotation
persistent identity: annotation1482868
display id: annotation1482868
version: 1
top level: BBa_I15014
location: https://synbiohub.org/public/igem/BBa_I15014/annotation1482868/range1482868/1
component: BBa_E0031

https://synbiohub.org/public/igem/BBa_I15014/annotation1482868/range1482868/1
type: Range
persistent identity: range1482868
display id: range1482868
version: 1
direction: direction/forward
top level: BBa_I15014
start: 1
end: 740
orientation: inline

ECFP
type: ComponentDefinition
persistent identity: BBa K105009
display id: BBa_K105009
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: ECFP, yeast optimized for fusion proteins
creator: Katja Karstens
created: 2008-10-11T11:00:00Z
modified: 2015-05-08T01:08:52Z
discontinued: false
dominant: false
experience: experience/None
group u list: _253_
m user id: 0
owner id: 3313
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Our BioBrick is cyan fluorescent protein which can be used to label proteins in yeast. Therefor it has specific characteristics of BioBrick part for fusion proteins (see design details).
mutable notes: To enable the fusion with other domains in frame the vector of this BioBrick has no base pair in between the restriction side and the BioBrick. Furthermore, this coding sequence does not include a start codon.<br\n> For more information about this issus, see:<br\n> Phillips, I.E. and Silver, P.A. "A new Biobrick Assembly Strategy Designed for Facile Protein Engineering." <br\n> DSpace http://hdl.handle.net/1721.1/32535 (2006).
mutable provenance: We changed the format of the ECFP coding part of BBa_E2020 into Phillips/Silver standard.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence: https://synbiohub.org/public/igem/BBa_K105009_sequence/1

BBa_K1899001
type: ComponentDefinition
persistent identity: BBa K1899001
display id: BBa_K1899001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Blue Fluorescent Protein mTagBFP generator (Medium RBS)
creator: iGEM2016_HKUST
created: 2016-09-19T11:00:00Z
modified: 2016-09-21T09:47:51Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2365_
m user id: 26069
owner id: 26069
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Improved version of BBa_K592023 by adding a terminator BBa_B1006 3???to the part.
mutable notes: NIL
mutable provenance: NIL
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_B1006
component: BBa_K592023
sequence annotation: BBa_B1006
sequence annotation: BBa_K592023
sequence: https://synbiohub.org/public/igem/BBa_K1899001_sequence/1

BBa_B1006
type: Component
persistent identity: component2484336
display id: component2484336
version: 1
top level: BBa_K1899001
access: public
definition: https://synbiohub.org/public/igem/BBa_B1006/1

BBa_K592023
type: Component
persistent identity: component2484331
display id: component2484331
version: 1
top level: BBa_K1899001
access: public
definition: https://synbiohub.org/public/igem/BBa_K592023/1

BBa_B1006
type: SequenceAnnotation
persistent identity: annotation2484336
display id: annotation2484336
version: 1
top level: BBa_K1899001
location: https://synbiohub.org/public/igem/BBa_K1899001/annotation2484336/range2484336/1
component: BBa_B1006

https://synbiohub.org/public/igem/BBa_K1899001/annotation2484336/range2484336/1
type: Range
persistent identity: range2484336
display id: range2484336
version: 1
direction: direction/reverse
top level: BBa_K1899001
start: 733
end: 771
orientation: inline

BBa_K592023
type: SequenceAnnotation
persistent identity: annotation2484331
display id: annotation2484331
version: 1
top level: BBa_K1899001
location: https://synbiohub.org/public/igem/BBa_K1899001/annotation2484331/range2484331/1
component: BBa_K592023

https://synbiohub.org/public/igem/BBa_K1899001/annotation2484331/range2484331/1
type: Range
persistent identity: range2484331
display id: range2484331
version: 1
direction: direction/reverse
top level: BBa_K1899001
start: 1
end: 724
orientation: inline

Zwf-Device
type: ComponentDefinition
persistent identity: BBa K1840007
display id: BBa_K1840007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Device: TT-zwf-mCherry
creator: Julia Anna Adrian
created: 2015-09-17T11:00:00Z
modified: 2015-09-18T02:16:57Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2266_
m user id: 26543
owner id: 26543
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This device acts as a detector for glucose (and certain derivatives of it). The double transcription terminator ensures, that only the gene downstream of the promotor zwf is influenced by it. The zwf promotor (BBa_K1840002) is contained in the genome of Pseudomonas putida and is situated in front of the enzyme zwf (glucose 6-phosphate dehydrogenase). The repressor HexR can bind the zwf promotor sequence and thus prevent the transcription of the zwf gene. In case the glucose derivative 2-Keto-3-deoxy-6-phosphogluconate is present, it binds to the promotor and leads to a subsequent conformational change. Hence, the repressor can no longer bind and the gene(s) downstream can be transcribed. In our device, the downstream gene is mCherry, codon optimized for Pseudomonas (BBa_K1840004). Therefore, in the presence of glucose, mCherry is expressed. In various experiments, we could see that this device is in deed working.
mutable notes: As promotor sequence, we assumed all DNA in front of the zwf gene up to the next gene, coding for the repressor HexR. Since it is encoded on the opposite strand, there is the possibility, that zwf functions as a promotor for this gene as well. Therefore the double terminator was needed.
mutable provenance: The double terminator was already registered in the iGEM catalogue. The zwf promotor comes from the genome of Pseudomonas putida. The mCherry is codon optimized for expression in Pseudomonas.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1840002
component: BBa_K1840004
component: BBa_B0014
sequence annotation: BBa_B0014
sequence annotation: BBa_K1840002
sequence annotation: BBa_K1840004
sequence: https://synbiohub.org/public/igem/BBa_K1840007_sequence/1

BBa_K1840002
type: Component
persistent identity: component2473635
display id: component2473635
version: 1
top level: Zwf-Device
access: public
definition: https://synbiohub.org/public/igem/BBa_K1840002/1

BBa_K1840004
type: Component
persistent identity: component2473636
display id: component2473636
version: 1
top level: Zwf-Device
access: public
definition: https://synbiohub.org/public/igem/BBa_K1840004/1

BBa_B0014
type: Component
persistent identity: component2473634
display id: component2473634
version: 1
top level: Zwf-Device
access: public
definition: https://synbiohub.org/public/igem/BBa_B0014/1

BBa_B0014
type: SequenceAnnotation
persistent identity: annotation2473634
display id: annotation2473634
version: 1
top level: Zwf-Device
location: https://synbiohub.org/public/igem/BBa_K1840007/annotation2473634/range2473634/1
component: BBa_B0014

https://synbiohub.org/public/igem/BBa_K1840007/annotation2473634/range2473634/1
type: Range
persistent identity: range2473634
display id: range2473634
version: 1
direction: direction/reverse
top level: Zwf-Device
start: 1
end: 95
orientation: inline

BBa_K1840002
type: SequenceAnnotation
persistent identity: annotation2473635
display id: annotation2473635
version: 1
top level: Zwf-Device
location: https://synbiohub.org/public/igem/BBa_K1840007/annotation2473635/range2473635/1
component: BBa_K1840002

https://synbiohub.org/public/igem/BBa_K1840007/annotation2473635/range2473635/1
type: Range
persistent identity: range2473635
display id: range2473635
version: 1
direction: direction/reverse
top level: Zwf-Device
start: 104
end: 305
orientation: inline

BBa_K1840004
type: SequenceAnnotation
persistent identity: annotation2473636
display id: annotation2473636
version: 1
top level: Zwf-Device
location: https://synbiohub.org/public/igem/BBa_K1840007/annotation2473636/range2473636/1
component: BBa_K1840004

https://synbiohub.org/public/igem/BBa_K1840007/annotation2473636/range2473636/1
type: Range
persistent identity: range2473636
display id: range2473636
version: 1
direction: direction/reverse
top level: Zwf-Device
start: 312
end: 1025
orientation: inline

BBa_K180014
type: ComponentDefinition
persistent identity: BBa K180014
display id: BBa_K180014
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Rock-paper-scissors - Paper primary plasmid
creator: Richard Wilson, Stephanie Chambers
created: 2009-10-20T11:00:00Z
modified: 2015-05-08T01:11:06Z
discontinued: false
dominant: false
experience: experience/None
group u list: _278_
m user id: 0
owner id: 4082
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: Composite part, consisting of BBa_K180005 and BBa_K180015, but with BBa_K180005 reading along the reverse strand, and BBa_K180015 reading along the forward strand. Forms the 'paper' primary plasmid in Team:Southampton's rock-paper-scissors project design.
mutable notes: BBa_K180005 reading along reverse strand, and BBa_K180015 reading along the forward strand to ensure the constitutive promoter BBa_J23119 (present in BBa_K180005) has no effect over GFP or RhlI expression in BBa_K180015.
mutable provenance: See relevant biobrick component parts (BBa_K180011 and BBa_K180012).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: conserved
sequence annotation: luxR
sequence annotation: K180015
sequence annotation: C0170
sequence annotation: B0034
sequence annotation: Ptac
sequence annotation: E0040
sequence annotation: B0015
sequence annotation: rhlI
sequence annotation: C0062
sequence annotation: GFPmut3b
sequence annotation: -10
sequence annotation: -35
sequence annotation: lacIq
sequence annotation: J23119
sequence annotation: K180005
sequence annotation: K180000
sequence: https://synbiohub.org/public/igem/BBa_K180014_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation2058332
display id: annotation2058332
version: 1
top level: BBa_K180014
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K180014/annotation2058332/range2058332/1

https://synbiohub.org/public/igem/BBa_K180014/annotation2058332/range2058332/1
type: Range
persistent identity: range2058332
display id: range2058332
version: 1
direction: direction/reverse
top level: BBa_K180014
start: 929
end: 932
orientation: inline

luxR
type: SequenceAnnotation
persistent identity: annotation2058326
display id: annotation2058326
version: 1
top level: BBa_K180014
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K180014/annotation2058326/range2058326/1

https://synbiohub.org/public/igem/BBa_K180014/annotation2058326/range2058326/1
type: Range
persistent identity: range2058326
display id: range2058326
version: 1
direction: direction/reverse
top level: BBa_K180014
start: 169
end: 918
orientation: inline

K180015
type: SequenceAnnotation
persistent identity: annotation2058321
display id: annotation2058321
version: 1
top level: BBa_K180014
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K180014/annotation2058321/range2058321/1

https://synbiohub.org/public/igem/BBa_K180014/annotation2058321/range2058321/1
type: Range
persistent identity: range2058321
display id: range2058321
version: 1
direction: direction/forward
top level: BBa_K180014
start: 988
end: 3952
orientation: inline

C0170
type: SequenceAnnotation
persistent identity: annotation2058341
display id: annotation2058341
version: 1
top level: BBa_K180014
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K180014/annotation2058341/range2058341/1

https://synbiohub.org/public/igem/BBa_K180014/annotation2058341/range2058341/1
type: Range
persistent identity: range2058341
display id: range2058341
version: 1
direction: direction/forward
top level: BBa_K180014
start: 3207
end: 3815
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation2058327
display id: annotation2058327
version: 1
top level: BBa_K180014
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K180014/annotation2058327/range2058327/1

https://synbiohub.org/public/igem/BBa_K180014/annotation2058327/range2058327/1
type: Range
persistent identity: range2058327
display id: range2058327
version: 1
direction: direction/forward
top level: BBa_K180014
start: 2443
end: 2454
orientation: inline

Ptac
type: SequenceAnnotation
persistent identity: annotation2058338
display id: annotation2058338
version: 1
top level: BBa_K180014
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K180014/annotation2058338/range2058338/1

https://synbiohub.org/public/igem/BBa_K180014/annotation2058338/range2058338/1
type: Range
persistent identity: range2058338
display id: range2058338
version: 1
direction: direction/forward
top level: BBa_K180014
start: 2312
end: 2339
orientation: inline

E0040
type: SequenceAnnotation
persistent identity: annotation2058339
display id: annotation2058339
version: 1
top level: BBa_K180014
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K180014/annotation2058339/range2058339/1

https://synbiohub.org/public/igem/BBa_K180014/annotation2058339/range2058339/1
type: Range
persistent identity: range2058339
display id: range2058339
version: 1
direction: direction/forward
top level: BBa_K180014
start: 2461
end: 3180
orientation: inline

B0015
type: SequenceAnnotation
persistent identity: annotation2058343
display id: annotation2058343
version: 1
top level: BBa_K180014
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K180014/annotation2058343/range2058343/1

https://synbiohub.org/public/igem/BBa_K180014/annotation2058343/range2058343/1
type: Range
persistent identity: range2058343
display id: range2058343
version: 1
direction: direction/forward
top level: BBa_K180014
start: 3824
end: 3952
orientation: inline

rhlI
type: SequenceAnnotation
persistent identity: annotation2058342
display id: annotation2058342
version: 1
top level: BBa_K180014
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K180014/annotation2058342/range2058342/1

https://synbiohub.org/public/igem/BBa_K180014/annotation2058342/range2058342/1
type: Range
persistent identity: range2058342
display id: range2058342
version: 1
direction: direction/forward
top level: BBa_K180014
start: 3207
end: 3815
orientation: inline

conserved
type: SequenceAnnotation
persistent identity: annotation2058330
display id: annotation2058330
version: 1
top level: BBa_K180014
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K180014/annotation2058330/range2058330/1

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type: Range
persistent identity: range2058330
display id: range2058330
version: 1
direction: direction/forward
top level: BBa_K180014
start: 3193
end: 3196
orientation: inline

C0062
type: SequenceAnnotation
persistent identity: annotation2058325
display id: annotation2058325
version: 1
top level: BBa_K180014
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K180014/annotation2058325/range2058325/1

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type: Range
persistent identity: range2058325
display id: range2058325
version: 1
direction: direction/reverse
top level: BBa_K180014
start: 163
end: 918
orientation: inline

GFPmut3b
type: SequenceAnnotation
persistent identity: annotation2058340
display id: annotation2058340
version: 1
top level: BBa_K180014
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K180014/annotation2058340/range2058340/1

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type: Range
persistent identity: range2058340
display id: range2058340
version: 1
direction: direction/forward
top level: BBa_K180014
start: 2461
end: 3180
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2058335
display id: annotation2058335
version: 1
top level: BBa_K180014
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K180014/annotation2058335/range2058335/1

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type: Range
persistent identity: range2058335
display id: range2058335
version: 1
direction: direction/reverse
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start: 951
end: 956
orientation: inline

conserved
type: SequenceAnnotation
persistent identity: annotation2058328
display id: annotation2058328
version: 1
top level: BBa_K180014
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K180014/annotation2058328/range2058328/1

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type: Range
persistent identity: range2058328
display id: range2058328
version: 1
direction: direction/forward
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start: 2447
end: 2450
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation2058329
display id: annotation2058329
version: 1
top level: BBa_K180014
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K180014/annotation2058329/range2058329/1

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type: Range
persistent identity: range2058329
display id: range2058329
version: 1
direction: direction/forward
top level: BBa_K180014
start: 3189
end: 3200
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2058334
display id: annotation2058334
version: 1
top level: BBa_K180014
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K180014/annotation2058334/range2058334/1

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type: Range
persistent identity: range2058334
display id: range2058334
version: 1
direction: direction/reverse
top level: BBa_K180014
start: 975
end: 979
orientation: inline

lacIq
type: SequenceAnnotation
persistent identity: annotation2058337
display id: annotation2058337
version: 1
top level: BBa_K180014
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K180014/annotation2058337/range2058337/1

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type: Range
persistent identity: range2058337
display id: range2058337
version: 1
direction: direction/forward
top level: BBa_K180014
start: 988
end: 2069
orientation: inline

J23119
type: SequenceAnnotation
persistent identity: annotation2058333
display id: annotation2058333
version: 1
top level: BBa_K180014
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K180014/annotation2058333/range2058333/1

https://synbiohub.org/public/igem/BBa_K180014/annotation2058333/range2058333/1
type: Range
persistent identity: range2058333
display id: range2058333
version: 1
direction: direction/reverse
top level: BBa_K180014
start: 945
end: 979
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation2058331
display id: annotation2058331
version: 1
top level: BBa_K180014
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K180014/annotation2058331/range2058331/1

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type: Range
persistent identity: range2058331
display id: range2058331
version: 1
direction: direction/reverse
top level: BBa_K180014
start: 925
end: 936
orientation: inline

K180005
type: SequenceAnnotation
persistent identity: annotation2058320
display id: annotation2058320
version: 1
top level: BBa_K180014
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K180014/annotation2058320/range2058320/1

https://synbiohub.org/public/igem/BBa_K180014/annotation2058320/range2058320/1
type: Range
persistent identity: range2058320
display id: range2058320
version: 1
direction: direction/reverse
top level: BBa_K180014
start: 1
end: 979
orientation: inline

K180000
type: SequenceAnnotation
persistent identity: annotation2058336
display id: annotation2058336
version: 1
top level: BBa_K180014
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K180014/annotation2058336/range2058336/1

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type: Range
persistent identity: range2058336
display id: range2058336
version: 1
direction: direction/forward
top level: BBa_K180014
start: 988
end: 2434
orientation: inline

B0015
type: SequenceAnnotation
persistent identity: annotation2058324
display id: annotation2058324
version: 1
top level: BBa_K180014
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K180014/annotation2058324/range2058324/1

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type: Range
persistent identity: range2058324
display id: range2058324
version: 1
direction: direction/reverse
top level: BBa_K180014
start: 1
end: 129
orientation: inline

BBa_K1031610
type: ComponentDefinition
persistent identity: BBa K1031610
display id: BBa_K1031610
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: NahR-pSal-sfGFP-Terminator
creator: He Shuaixin
created: 2013-09-08T11:00:00Z
modified: 2015-05-08T01:08:47Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1338_
m user id: 0
owner id: 15843
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: NahR-sfGFP-TT
mutable notes: molecular cloning
mutable provenance: part, lab made
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
sequence annotation: pSal
sequence annotation: NahR
sequence annotation: B0015
sequence annotation: sfGFP
sequence: https://synbiohub.org/public/igem/BBa_K1031610_sequence/1

pSal
type: SequenceAnnotation
persistent identity: annotation2360410
display id: annotation2360410
version: 1
top level: BBa_K1031610
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1031610/annotation2360410/range2360410/1

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type: Range
persistent identity: range2360410
display id: range2360410
version: 1
direction: direction/forward
top level: BBa_K1031610
start: 1192
end: 1262
orientation: inline

NahR
type: SequenceAnnotation
persistent identity: annotation2360409
display id: annotation2360409
version: 1
top level: BBa_K1031610
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1031610/annotation2360409/range2360409/1

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type: Range
persistent identity: range2360409
display id: range2360409
version: 1
direction: direction/reverse
top level: BBa_K1031610
start: 211
end: 1113
orientation: inline

B0015
type: SequenceAnnotation
persistent identity: annotation2360412
display id: annotation2360412
version: 1
top level: BBa_K1031610
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1031610/annotation2360412/range2360412/1

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type: Range
persistent identity: range2360412
display id: range2360412
version: 1
direction: direction/forward
top level: BBa_K1031610
start: 2004
end: 2132
orientation: inline

sfGFP
type: SequenceAnnotation
persistent identity: annotation2360411
display id: annotation2360411
version: 1
top level: BBa_K1031610
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1031610/annotation2360411/range2360411/1

https://synbiohub.org/public/igem/BBa_K1031610/annotation2360411/range2360411/1
type: Range
persistent identity: range2360411
display id: range2360411
version: 1
direction: direction/forward
top level: BBa_K1031610
start: 1275
end: 1997
orientation: inline

BBa_K1418041
type: ComponentDefinition
persistent identity: BBa K1418041
display id: BBa_K1418041
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CFP(ns)
creator: Cody Maxfield
created: 2014-10-07T11:00:00Z
modified: 2016-01-26T02:12:01Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1796_
m user id: 4206
owner id: 13965
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: CFP
mutable notes: RFC 23
mutable provenance: CFP
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: atg
sequence annotation: CFP
sequence: https://synbiohub.org/public/igem/BBa_K1418041_sequence/1

atg
type: SequenceAnnotation
persistent identity: annotation2421390
display id: annotation2421390
version: 1
top level: BBa_K1418041
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1418041/annotation2421390/range2421390/1

https://synbiohub.org/public/igem/BBa_K1418041/annotation2421390/range2421390/1
type: Range
persistent identity: range2421390
display id: range2421390
version: 1
direction: direction/forward
top level: BBa_K1418041
start: 1
end: 3
orientation: inline

CFP
type: SequenceAnnotation
persistent identity: annotation2421402
display id: annotation2421402
version: 1
top level: BBa_K1418041
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1418041/annotation2421402/range2421402/1

https://synbiohub.org/public/igem/BBa_K1418041/annotation2421402/range2421402/1
type: Range
persistent identity: range2421402
display id: range2421402
version: 1
direction: direction/forward
top level: BBa_K1418041
start: 4
end: 717
orientation: inline

BBa_K283035
type: ComponentDefinition
persistent identity: BBa K283035
display id: BBa_K283035
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RFP-double terminators
creator: Shi Lei, Nelson Chu
created: 2009-10-15T11:00:00Z
modified: 2015-05-08T01:11:48Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _383_
m user id: 0
owner id: 5494
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: cI coding protein on plasmid backbone J61002
mutable notes: BBa_K283035
mutable provenance: From the registry
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0010
component: BBa_B0012
component: BBa_E1010
sequence annotation: BBa_B0012
sequence annotation: BBa_B0010
sequence annotation: BBa_E1010
sequence: https://synbiohub.org/public/igem/BBa_K283035_sequence/1

BBa_B0010
type: Component
persistent identity: component2246777
display id: component2246777
version: 1
top level: BBa_K283035
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
type: Component
persistent identity: component2246779
display id: component2246779
version: 1
top level: BBa_K283035
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_E1010
type: Component
persistent identity: component2246776
display id: component2246776
version: 1
top level: BBa_K283035
access: public
definition: mRFP1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2246779
display id: annotation2246779
version: 1
top level: BBa_K283035
location: https://synbiohub.org/public/igem/BBa_K283035/annotation2246779/range2246779/1
component: BBa_B0012

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type: Range
persistent identity: range2246779
display id: range2246779
version: 1
direction: direction/forward
top level: BBa_K283035
start: 803
end: 843
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2246777
display id: annotation2246777
version: 1
top level: BBa_K283035
location: https://synbiohub.org/public/igem/BBa_K283035/annotation2246777/range2246777/1
component: BBa_B0010

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type: Range
persistent identity: range2246777
display id: range2246777
version: 1
direction: direction/forward
top level: BBa_K283035
start: 715
end: 794
orientation: inline

BBa_E1010
type: SequenceAnnotation
persistent identity: annotation2246776
display id: annotation2246776
version: 1
top level: BBa_K283035
location: https://synbiohub.org/public/igem/BBa_K283035/annotation2246776/range2246776/1
component: BBa_E1010

https://synbiohub.org/public/igem/BBa_K283035/annotation2246776/range2246776/1
type: Range
persistent identity: range2246776
display id: range2246776
version: 1
direction: direction/reverse
top level: BBa_K283035
start: 1
end: 706
orientation: inline

BBa_K1486010
type: ComponentDefinition
persistent identity: BBa K1486010
display id: BBa_K1486010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CxpR & Split IFP1.4 [Nterm + Cterm]
creator: iGem EPFL 2014
created: 2014-08-26T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part has two fused proteins downstream of an arabinose-inducible promoter: CpxR-IFP[1] and CpxR-IFP[2]. This construct was made in all orientations: - CpxR-IFP[1] + CpxR-IFP[2] (BBa_K1286008) - IFP[1]-CpxR + CpxR-IFP[2] (BBa_K1486010) - CpxR-IFP[1] + IFP[2]-CpxR (BBa_K1486011)
mutable notes: All orientations were made, so all dimerizations could be tested (see main page for biobrick numbers). Flexible linkers were used for each of the fused proteins, and an RBS was put before each one of them.
mutable provenance: From Michnick Article. To Edit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_I0500
component: BBa_K1486007
component: BBa_K1486000
component: BBa_B0034
component: BBa_K1486004
component: BBa_K1486006
sequence annotation: BBa_K1486000
sequence annotation: BBa_B0034
sequence annotation: BBa_K1486004
sequence annotation: BBa_K1486006
sequence annotation: BBa_I0500
sequence annotation: BBa_K1486007
sequence: https://synbiohub.org/public/igem/BBa_K1486010_sequence/1

BBa_I0500
type: Component
persistent identity: component2424984
display id: component2424984
version: 1
top level: BBa_K1486010
access: public
definition: https://synbiohub.org/public/igem/BBa_I0500/1

BBa_K1486007
type: Component
persistent identity: component2425006
display id: component2425006
version: 1
top level: BBa_K1486010
access: public
definition: BBa_K1486007

BBa_K1486000
type: Component
persistent identity: component2425001
display id: component2425001
version: 1
top level: BBa_K1486010
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486000/1

BBa_K1486000
type: Component
persistent identity: component2424995
display id: component2424995
version: 1
top level: BBa_K1486010
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486000/1

BBa_B0034
type: Component
persistent identity: component2424986
display id: component2424986
version: 1
top level: BBa_K1486010
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K1486004
type: Component
persistent identity: component2425002
display id: component2425002
version: 1
top level: BBa_K1486010
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486004/1

BBa_K1486004
type: Component
persistent identity: component2424991
display id: component2424991
version: 1
top level: BBa_K1486010
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486004/1

BBa_B0034
type: Component
persistent identity: component2424997
display id: component2424997
version: 1
top level: BBa_K1486010
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K1486006
type: Component
persistent identity: component2424990
display id: component2424990
version: 1
top level: BBa_K1486010
access: public
definition: BBa_K1486006

BBa_K1486000
type: SequenceAnnotation
persistent identity: annotation2425001
display id: annotation2425001
version: 1
top level: BBa_K1486010
location: https://synbiohub.org/public/igem/BBa_K1486010/annotation2425001/range2425001/1
component: BBa_K1486000

https://synbiohub.org/public/igem/BBa_K1486010/annotation2425001/range2425001/1
type: Range
persistent identity: range2425001
display id: range2425001
version: 1
direction: direction/reverse
top level: BBa_K1486010
start: 2372
end: 3073
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2424986
display id: annotation2424986
version: 1
top level: BBa_K1486010
location: https://synbiohub.org/public/igem/BBa_K1486010/annotation2424986/range2424986/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1486010/annotation2424986/range2424986/1
type: Range
persistent identity: range2424986
display id: range2424986
version: 1
direction: direction/reverse
top level: BBa_K1486010
start: 1211
end: 1222
orientation: inline

BBa_K1486004
type: SequenceAnnotation
persistent identity: annotation2424991
display id: annotation2424991
version: 1
top level: BBa_K1486010
location: https://synbiohub.org/public/igem/BBa_K1486010/annotation2424991/range2424991/1
component: BBa_K1486004

https://synbiohub.org/public/igem/BBa_K1486010/annotation2424991/range2424991/1
type: Range
persistent identity: range2424991
display id: range2424991
version: 1
direction: direction/reverse
top level: BBa_K1486010
start: 1628
end: 1657
orientation: inline

BBa_K1486006
type: SequenceAnnotation
persistent identity: annotation2424990
display id: annotation2424990
version: 1
top level: BBa_K1486010
location: https://synbiohub.org/public/igem/BBa_K1486010/annotation2424990/range2424990/1
component: BBa_K1486006

https://synbiohub.org/public/igem/BBa_K1486010/annotation2424990/range2424990/1
type: Range
persistent identity: range2424990
display id: range2424990
version: 1
direction: direction/reverse
top level: BBa_K1486010
start: 1223
end: 1627
orientation: inline

BBa_I0500
type: SequenceAnnotation
persistent identity: annotation2424984
display id: annotation2424984
version: 1
top level: BBa_K1486010
location: https://synbiohub.org/public/igem/BBa_K1486010/annotation2424984/range2424984/1
component: BBa_I0500

https://synbiohub.org/public/igem/BBa_K1486010/annotation2424984/range2424984/1
type: Range
persistent identity: range2424984
display id: range2424984
version: 1
direction: direction/reverse
top level: BBa_K1486010
start: 1
end: 1210
orientation: inline

BBa_K1486007
type: SequenceAnnotation
persistent identity: annotation2425006
display id: annotation2425006
version: 1
top level: BBa_K1486010
location: https://synbiohub.org/public/igem/BBa_K1486010/annotation2425006/range2425006/1
component: BBa_K1486007

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type: Range
persistent identity: range2425006
display id: range2425006
version: 1
direction: direction/reverse
top level: BBa_K1486010
start: 3104
end: 3688
orientation: inline

BBa_K1486000
type: SequenceAnnotation
persistent identity: annotation2424995
display id: annotation2424995
version: 1
top level: BBa_K1486010
location: https://synbiohub.org/public/igem/BBa_K1486010/annotation2424995/range2424995/1
component: BBa_K1486000

https://synbiohub.org/public/igem/BBa_K1486010/annotation2424995/range2424995/1
type: Range
persistent identity: range2424995
display id: range2424995
version: 1
direction: direction/reverse
top level: BBa_K1486010
start: 1658
end: 2359
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2424997
display id: annotation2424997
version: 1
top level: BBa_K1486010
location: https://synbiohub.org/public/igem/BBa_K1486010/annotation2424997/range2424997/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1486010/annotation2424997/range2424997/1
type: Range
persistent identity: range2424997
display id: range2424997
version: 1
direction: direction/reverse
top level: BBa_K1486010
start: 2360
end: 2371
orientation: inline

BBa_K1486004
type: SequenceAnnotation
persistent identity: annotation2425002
display id: annotation2425002
version: 1
top level: BBa_K1486010
location: https://synbiohub.org/public/igem/BBa_K1486010/annotation2425002/range2425002/1
component: BBa_K1486004

https://synbiohub.org/public/igem/BBa_K1486010/annotation2425002/range2425002/1
type: Range
persistent identity: range2425002
display id: range2425002
version: 1
direction: direction/reverse
top level: BBa_K1486010
start: 3074
end: 3103
orientation: inline

eGFP-PTS1
type: ComponentDefinition
persistent identity: BBa K678002
display id: BBa_K678002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: eGFP-PTS1 for mammalian cells
creator: DTU-Denmark-2
created: 2011-09-01T11:00:00Z
modified: 2015-05-08T01:13:04Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _882_
m user id: 0
owner id: 8341
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: Vector expressing eGFP from the CMV promoter with the BGH polyA tail and with a hygromycin resistance cassette
mutable notes: Compatible with the Plug'n'Play with DNA assembly system.
mutable provenance: Source to be announced
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
sequence annotation: Linker 1
sequence annotation: Kozak
sequence annotation: Linker 4
sequence annotation: CMV
sequence annotation: Linker 2
sequence annotation: Linker 6
sequence annotation: eGFP
sequence annotation: BGH pA
sequence annotation: Linker 5
sequence annotation: Hygromycin cassette
sequence annotation: PTS1
sequence: https://synbiohub.org/public/igem/BBa_K678002_sequence/1

Linker 1
type: SequenceAnnotation
persistent identity: annotation2126912
display id: annotation2126912
version: 1
top level: eGFP-PTS1
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678002/annotation2126912/range2126912/1

https://synbiohub.org/public/igem/BBa_K678002/annotation2126912/range2126912/1
type: Range
persistent identity: range2126912
display id: range2126912
version: 1
direction: direction/forward
top level: eGFP-PTS1
start: 1
end: 8
orientation: inline

Kozak
type: SequenceAnnotation
persistent identity: annotation2126935
display id: annotation2126935
version: 1
top level: eGFP-PTS1
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678002/annotation2126935/range2126935/1

https://synbiohub.org/public/igem/BBa_K678002/annotation2126935/range2126935/1
type: Range
persistent identity: range2126935
display id: range2126935
version: 1
direction: direction/forward
top level: eGFP-PTS1
start: 672
end: 682
orientation: inline

Linker 4
type: SequenceAnnotation
persistent identity: annotation2126937
display id: annotation2126937
version: 1
top level: eGFP-PTS1
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678002/annotation2126937/range2126937/1

https://synbiohub.org/public/igem/BBa_K678002/annotation2126937/range2126937/1
type: Range
persistent identity: range2126937
display id: range2126937
version: 1
direction: direction/forward
top level: eGFP-PTS1
start: 1407
end: 1414
orientation: inline

CMV
type: SequenceAnnotation
persistent identity: annotation2126913
display id: annotation2126913
version: 1
top level: eGFP-PTS1
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K678002/annotation2126913/range2126913/1

https://synbiohub.org/public/igem/BBa_K678002/annotation2126913/range2126913/1
type: Range
persistent identity: range2126913
display id: range2126913
version: 1
direction: direction/forward
top level: eGFP-PTS1
start: 9
end: 662
orientation: inline

Linker 2
type: SequenceAnnotation
persistent identity: annotation2126914
display id: annotation2126914
version: 1
top level: eGFP-PTS1
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678002/annotation2126914/range2126914/1

https://synbiohub.org/public/igem/BBa_K678002/annotation2126914/range2126914/1
type: Range
persistent identity: range2126914
display id: range2126914
version: 1
direction: direction/forward
top level: eGFP-PTS1
start: 663
end: 671
orientation: inline

Linker 6
type: SequenceAnnotation
persistent identity: annotation2126941
display id: annotation2126941
version: 1
top level: eGFP-PTS1
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678002/annotation2126941/range2126941/1

https://synbiohub.org/public/igem/BBa_K678002/annotation2126941/range2126941/1
type: Range
persistent identity: range2126941
display id: range2126941
version: 1
direction: direction/forward
top level: eGFP-PTS1
start: 3399
end: 3406
orientation: inline

eGFP
type: SequenceAnnotation
persistent identity: annotation2126934
display id: annotation2126934
version: 1
top level: eGFP-PTS1
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K678002/annotation2126934/range2126934/1

https://synbiohub.org/public/igem/BBa_K678002/annotation2126934/range2126934/1
type: Range
persistent identity: range2126934
display id: range2126934
version: 1
direction: direction/forward
top level: eGFP-PTS1
start: 679
end: 1394
orientation: inline

BGH pA
type: SequenceAnnotation
persistent identity: annotation2126938
display id: annotation2126938
version: 1
top level: eGFP-PTS1
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_K678002/annotation2126938/range2126938/1

https://synbiohub.org/public/igem/BBa_K678002/annotation2126938/range2126938/1
type: Range
persistent identity: range2126938
display id: range2126938
version: 1
direction: direction/forward
top level: eGFP-PTS1
start: 1415
end: 1639
orientation: inline

Linker 5
type: SequenceAnnotation
persistent identity: annotation2126939
display id: annotation2126939
version: 1
top level: eGFP-PTS1
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678002/annotation2126939/range2126939/1

https://synbiohub.org/public/igem/BBa_K678002/annotation2126939/range2126939/1
type: Range
persistent identity: range2126939
display id: range2126939
version: 1
direction: direction/forward
top level: eGFP-PTS1
start: 1640
end: 1647
orientation: inline

Hygromycin cassette
type: SequenceAnnotation
persistent identity: annotation2126940
display id: annotation2126940
version: 1
top level: eGFP-PTS1
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678002/annotation2126940/range2126940/1

https://synbiohub.org/public/igem/BBa_K678002/annotation2126940/range2126940/1
type: Range
persistent identity: range2126940
display id: range2126940
version: 1
direction: direction/forward
top level: eGFP-PTS1
start: 1648
end: 3398
orientation: inline

PTS1
type: SequenceAnnotation
persistent identity: annotation2126936
display id: annotation2126936
version: 1
top level: eGFP-PTS1
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K678002/annotation2126936/range2126936/1

https://synbiohub.org/public/igem/BBa_K678002/annotation2126936/range2126936/1
type: Range
persistent identity: range2126936
display id: range2126936
version: 1
direction: direction/forward
top level: eGFP-PTS1
start: 1395
end: 1406
orientation: inline

HisTag-GFP
type: ComponentDefinition
persistent identity: BBa K1602021
display id: BBa_K1602021
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Green fluorescent protein (GFP) with N-terminal His-Tag
creator: Jasmin Weber, Michelle Zoeller, Marcus Geissler, Stefan Bohn, Simon Linder, Sebastian Barthel
created: 2015-09-15T11:00:00Z
modified: 2016-01-25T01:10:25Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2019_
m user id: 0
m user id: 4206
owner id: 22022
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1602021_sequence/1

BBa_K1641224
type: ComponentDefinition
persistent identity: BBa K1641224
display id: BBa_K1641224
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: mCherry-ssrA
creator: Jianheng Liu
created: 2015-09-13T11:00:00Z
modified: 2016-01-25T01:06:32Z
discontinued: false
dominant: false
group u list: _2058_
m user id: 4206
owner id: 23346
owning group id: 9
status: status/Planning
bookmark: false
mutable description: mCherry (BBa_J06504) with ssrA tag（BBa_M0052）
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1641224_sequence/1

BBa_K1468002
type: ComponentDefinition
persistent identity: BBa K1468002
display id: BBa_K1468002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pJ23110 + gene encoding ZsGreen
creator: Pedro Luis Dorado Morales
created: 2014-10-02T11:00:00Z
modified: 2015-05-08T01:10:38Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1847_
m user id: 0
owner id: 11756
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: -
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence: https://synbiohub.org/public/igem/BBa_K1468002_sequence/1

BBa_J52028
type: ComponentDefinition
persistent identity: BBa J52028
display id: BBa_J52028
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: green fluorescent protein derived from A. victoria GFP (SwissProt: P42212) with PEST191 tag
creator: Jelka Pohar
created: 2006-10-14T11:00:00Z
modified: 2015-08-31T03:54:04Z
discontinued: false
dominant: false
group u list: _80_
m user id: 0
owner id: 855
owning group id: 80
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: GFP-linker-PEST191 is a part composed of GFP (as a reporter) linked with PEST191 through six aminoacid long protein linker. PEST191 is a sequence that leads towards degradation of protein linked with this sequence. It's estimated half-time is 2h.
mutable notes: GFP-linker-PEST191 is made by PCR overlap extension technique.
mutable provenance: GFP- PEST191-
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: linker
sequence annotation: PEST191 (degradation flag)
sequence annotation: start codon
sequence annotation: stop codon
sequence annotation: GFP-linker-PEST191
sequence annotation: GFP
sequence: https://synbiohub.org/public/igem/BBa_J52028_sequence/1

linker
type: SequenceAnnotation
persistent identity: annotation1903015
display id: annotation1903015
version: 1
top level: BBa_J52028
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_J52028/annotation1903015/range1903015/1

https://synbiohub.org/public/igem/BBa_J52028/annotation1903015/range1903015/1
type: Range
persistent identity: range1903015
display id: range1903015
version: 1
direction: direction/forward
top level: BBa_J52028
start: 718
end: 735
orientation: inline

PEST191 (degradation flag)
type: SequenceAnnotation
persistent identity: annotation1903016
display id: annotation1903016
version: 1
top level: BBa_J52028
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_J52028/annotation1903016/range1903016/1

https://synbiohub.org/public/igem/BBa_J52028/annotation1903016/range1903016/1
type: Range
persistent identity: range1903016
display id: range1903016
version: 1
direction: direction/forward
top level: BBa_J52028
start: 736
end: 1221
orientation: inline

start codon
type: SequenceAnnotation
persistent identity: annotation1908104
display id: annotation1908104
version: 1
top level: BBa_J52028
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_J52028/annotation1908104/range1908104/1

https://synbiohub.org/public/igem/BBa_J52028/annotation1908104/range1908104/1
type: Range
persistent identity: range1908104
display id: range1908104
version: 1
direction: direction/forward
top level: BBa_J52028
start: 1
end: 3
orientation: inline

stop codon
type: SequenceAnnotation
persistent identity: annotation1908105
display id: annotation1908105
version: 1
top level: BBa_J52028
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_J52028/annotation1908105/range1908105/1

https://synbiohub.org/public/igem/BBa_J52028/annotation1908105/range1908105/1
type: Range
persistent identity: range1908105
display id: range1908105
version: 1
direction: direction/forward
top level: BBa_J52028
start: 1219
end: 1221
orientation: inline

GFP-linker-PEST191
type: SequenceAnnotation
persistent identity: annotation1903017
display id: annotation1903017
version: 1
top level: BBa_J52028
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_J52028/annotation1903017/range1903017/1

https://synbiohub.org/public/igem/BBa_J52028/annotation1903017/range1903017/1
type: Range
persistent identity: range1903017
display id: range1903017
version: 1
direction: direction/forward
top level: BBa_J52028
start: 1
end: 1221
orientation: inline

GFP
type: SequenceAnnotation
persistent identity: annotation1903014
display id: annotation1903014
version: 1
top level: BBa_J52028
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J52028/annotation1903014/range1903014/1

https://synbiohub.org/public/igem/BBa_J52028/annotation1903014/range1903014/1
type: Range
persistent identity: range1903014
display id: range1903014
version: 1
direction: direction/forward
top level: BBa_J52028
start: 1
end: 717
orientation: inline

BBa_K1468000
type: ComponentDefinition
persistent identity: BBa K1468000
display id: BBa_K1468000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pJ23104 + gene encoding ZsGreen
creator: Pedro Luis Dorado Morales
created: 2014-10-02T11:00:00Z
modified: 2015-05-08T01:10:38Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1847_
m user id: 0
owner id: 11756
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: -
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: ZsGreen protein
sequence annotation: BBa_K1468000
sequence annotation: BBa_J23104
sequence annotation: STOP
sequence annotation: J23104p
sequence annotation: ZsGreeen
sequence annotation: START
sequence annotation: BBa_E0040
sequence: https://synbiohub.org/public/igem/BBa_K1468000_sequence/1

ZsGreen protein
type: SequenceAnnotation
persistent identity: annotation2418059
display id: annotation2418059
version: 1
top level: BBa_K1468000
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1468000/annotation2418059/range2418059/1

https://synbiohub.org/public/igem/BBa_K1468000/annotation2418059/range2418059/1
type: Range
persistent identity: range2418059
display id: range2418059
version: 1
direction: direction/forward
top level: BBa_K1468000
start: 34
end: 725
orientation: inline

BBa_K1468000
type: SequenceAnnotation
persistent identity: annotation2418810
display id: annotation2418810
version: 1
top level: BBa_K1468000
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1468000/annotation2418810/range2418810/1

https://synbiohub.org/public/igem/BBa_K1468000/annotation2418810/range2418810/1
type: Range
persistent identity: range2418810
display id: range2418810
version: 1
direction: direction/forward
top level: BBa_K1468000
start: 1
end: 725
orientation: inline

BBa_J23104
type: SequenceAnnotation
persistent identity: annotation2418058
display id: annotation2418058
version: 1
top level: BBa_K1468000
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1468000/annotation2418058/range2418058/1

https://synbiohub.org/public/igem/BBa_K1468000/annotation2418058/range2418058/1
type: Range
persistent identity: range2418058
display id: range2418058
version: 1
direction: direction/forward
top level: BBa_K1468000
start: 1
end: 33
orientation: inline

STOP
type: SequenceAnnotation
persistent identity: annotation2418064
display id: annotation2418064
version: 1
top level: BBa_K1468000
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1468000/annotation2418064/range2418064/1

https://synbiohub.org/public/igem/BBa_K1468000/annotation2418064/range2418064/1
type: Range
persistent identity: range2418064
display id: range2418064
version: 1
direction: direction/forward
top level: BBa_K1468000
start: 725
end: 725
orientation: inline

J23104p
type: SequenceAnnotation
persistent identity: annotation2418030
display id: annotation2418030
version: 1
top level: BBa_K1468000
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1468000/annotation2418030/range2418030/1

https://synbiohub.org/public/igem/BBa_K1468000/annotation2418030/range2418030/1
type: Range
persistent identity: range2418030
display id: range2418030
version: 1
direction: direction/forward
top level: BBa_K1468000
start: 1
end: 33
orientation: inline

ZsGreeen
type: SequenceAnnotation
persistent identity: annotation2418062
display id: annotation2418062
version: 1
top level: BBa_K1468000
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1468000/annotation2418062/range2418062/1

https://synbiohub.org/public/igem/BBa_K1468000/annotation2418062/range2418062/1
type: Range
persistent identity: range2418062
display id: range2418062
version: 1
direction: direction/forward
top level: BBa_K1468000
start: 34
end: 725
orientation: inline

START
type: SequenceAnnotation
persistent identity: annotation2418061
display id: annotation2418061
version: 1
top level: BBa_K1468000
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1468000/annotation2418061/range2418061/1

https://synbiohub.org/public/igem/BBa_K1468000/annotation2418061/range2418061/1
type: Range
persistent identity: range2418061
display id: range2418061
version: 1
direction: direction/forward
top level: BBa_K1468000
start: 34
end: 36
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation2418060
display id: annotation2418060
version: 1
top level: BBa_K1468000
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1468000/annotation2418060/range2418060/1

https://synbiohub.org/public/igem/BBa_K1468000/annotation2418060/range2418060/1
type: Range
persistent identity: range2418060
display id: range2418060
version: 1
direction: direction/forward
top level: BBa_K1468000
start: 34
end: 725
orientation: inline

BBa_K125810
type: ComponentDefinition
persistent identity: BBa K125810
display id: BBa_K125810
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: slr2016 signal sequence + GFP fusion for secretion of GFP
creator: Grace Kwan
created: 2008-10-27T12:00:00Z
modified: 2015-05-08T01:09:45Z
discontinued: false
dominant: false
group u list: _179_
m user id: 0
owner id: 3229
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Ligation of the slr2016 and GFP fusion biobricks.
mutable notes: GFP had to be a fusion brick so that translation would continue in frame.
mutable provenance: ''slr2016'' signal sequence was derived from ''Synechocystis'' sp. PCC 6803 while GFP fusion was modified from the BioBrick part BBa_E0040.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_K125310
component: BBa_K125500
sequence annotation: BBa_K125500
sequence annotation: BBa_K125310
sequence: https://synbiohub.org/public/igem/BBa_K125810_sequence/1

BBa_K125310
type: Component
persistent identity: component1992520
display id: component1992520
version: 1
top level: BBa_K125810
access: public
definition: https://synbiohub.org/public/igem/BBa_K125310/1

BBa_K125500
type: Component
persistent identity: component1992522
display id: component1992522
version: 1
top level: BBa_K125810
access: public
definition: BBa_K125500

BBa_K125500
type: SequenceAnnotation
persistent identity: annotation1992522
display id: annotation1992522
version: 1
top level: BBa_K125810
location: https://synbiohub.org/public/igem/BBa_K125810/annotation1992522/range1992522/1
component: BBa_K125500

https://synbiohub.org/public/igem/BBa_K125810/annotation1992522/range1992522/1
type: Range
persistent identity: range1992522
display id: range1992522
version: 1
direction: direction/reverse
top level: BBa_K125810
start: 62
end: 779
orientation: inline

BBa_K125310
type: SequenceAnnotation
persistent identity: annotation1992520
display id: annotation1992520
version: 1
top level: BBa_K125810
location: https://synbiohub.org/public/igem/BBa_K125810/annotation1992520/range1992520/1
component: BBa_K125310

https://synbiohub.org/public/igem/BBa_K125810/annotation1992520/range1992520/1
type: Range
persistent identity: range1992520
display id: range1992520
version: 1
direction: direction/reverse
top level: BBa_K125810
start: 1
end: 53
orientation: inline

BBa_K879313
type: ComponentDefinition
persistent identity: BBa K879313
display id: BBa_K879313
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Yellow with promoter 3
creator: Nien-Tsu Tseng
created: 2012-09-30T11:00:00Z
modified: 2015-05-08T01:13:39Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1141_
m user id: 0
owner id: 13537
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: Yellow with promoter 3
mutable notes: .
mutable provenance: .
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K879021
component: BBa_K879092
component: BBa_K879091
component: BBa_K879013
component: BBa_K879033
sequence annotation: BBa_K879033
sequence annotation: BBa_K879013
sequence annotation: BBa_K879021
sequence annotation: BBa_K879092
sequence annotation: BBa_K879091
sequence: https://synbiohub.org/public/igem/BBa_K879313_sequence/1

BBa_K879021
type: Component
persistent identity: component2205042
display id: component2205042
version: 1
top level: BBa_K879313
access: public
definition: https://synbiohub.org/public/igem/BBa_K879021/1

BBa_K879092
type: Component
persistent identity: component2205041
display id: component2205041
version: 1
top level: BBa_K879313
access: public
definition: https://synbiohub.org/public/igem/BBa_K879092/1

BBa_K879091
type: Component
persistent identity: component2205039
display id: component2205039
version: 1
top level: BBa_K879313
access: public
definition: https://synbiohub.org/public/igem/BBa_K879091/1

BBa_K879013
type: Component
persistent identity: component2205043
display id: component2205043
version: 1
top level: BBa_K879313
access: public
definition: https://synbiohub.org/public/igem/BBa_K879013/1

BBa_K879033
type: Component
persistent identity: component2205040
display id: component2205040
version: 1
top level: BBa_K879313
access: public
definition: https://synbiohub.org/public/igem/BBa_K879033/1

BBa_K879033
type: SequenceAnnotation
persistent identity: annotation2205040
display id: annotation2205040
version: 1
top level: BBa_K879313
location: https://synbiohub.org/public/igem/BBa_K879313/annotation2205040/range2205040/1
component: BBa_K879033

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type: Range
persistent identity: range2205040
display id: range2205040
version: 1
direction: direction/reverse
top level: BBa_K879313
start: 37
end: 65
orientation: inline

BBa_K879013
type: SequenceAnnotation
persistent identity: annotation2205043
display id: annotation2205043
version: 1
top level: BBa_K879313
location: https://synbiohub.org/public/igem/BBa_K879313/annotation2205043/range2205043/1
component: BBa_K879013

https://synbiohub.org/public/igem/BBa_K879313/annotation2205043/range2205043/1
type: Range
persistent identity: range2205043
display id: range2205043
version: 1
direction: direction/reverse
top level: BBa_K879313
start: 101
end: 799
orientation: inline

BBa_K879021
type: SequenceAnnotation
persistent identity: annotation2205042
display id: annotation2205042
version: 1
top level: BBa_K879313
location: https://synbiohub.org/public/igem/BBa_K879313/annotation2205042/range2205042/1
component: BBa_K879021

https://synbiohub.org/public/igem/BBa_K879313/annotation2205042/range2205042/1
type: Range
persistent identity: range2205042
display id: range2205042
version: 1
direction: direction/reverse
top level: BBa_K879313
start: 75
end: 100
orientation: inline

BBa_K879092
type: SequenceAnnotation
persistent identity: annotation2205041
display id: annotation2205041
version: 1
top level: BBa_K879313
location: https://synbiohub.org/public/igem/BBa_K879313/annotation2205041/range2205041/1
component: BBa_K879092

https://synbiohub.org/public/igem/BBa_K879313/annotation2205041/range2205041/1
type: Range
persistent identity: range2205041
display id: range2205041
version: 1
direction: direction/reverse
top level: BBa_K879313
start: 66
end: 74
orientation: inline

BBa_K879091
type: SequenceAnnotation
persistent identity: annotation2205039
display id: annotation2205039
version: 1
top level: BBa_K879313
location: https://synbiohub.org/public/igem/BBa_K879313/annotation2205039/range2205039/1
component: BBa_K879091

https://synbiohub.org/public/igem/BBa_K879313/annotation2205039/range2205039/1
type: Range
persistent identity: range2205039
display id: range2205039
version: 1
direction: direction/reverse
top level: BBa_K879313
start: 1
end: 36
orientation: inline

eCFP-GOI
type: ComponentDefinition
persistent identity: BBa K678048
display id: BBa_K678048
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: eCFP-GOI
creator: DTU-Denmark-2
created: 2011-09-12T11:00:00Z
modified: 2015-05-08T01:13:04Z
discontinued: false
dominant: false
experience: experience/None
group u list: _882_
m user id: 0
owner id: 10556
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part was designed for the Plug'n'Play with DNA assembly standard (RFC 80), therefore this part contains tails at the 5' and 3' ends as shown on the figure below.
mutable notes: No
mutable provenance: The Danish Cancer Society
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: Linker 2
sequence annotation: eCFP-GOI
sequence annotation: Kosaz
sequence: https://synbiohub.org/public/igem/BBa_K678048_sequence/1

Linker 2
type: SequenceAnnotation
persistent identity: annotation2127668
display id: annotation2127668
version: 1
top level: eCFP-GOI
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678048/annotation2127668/range2127668/1

https://synbiohub.org/public/igem/BBa_K678048/annotation2127668/range2127668/1
type: Range
persistent identity: range2127668
display id: range2127668
version: 1
direction: direction/forward
top level: eCFP-GOI
start: 1
end: 8
orientation: inline

eCFP-GOI
type: SequenceAnnotation
persistent identity: annotation2127669
display id: annotation2127669
version: 1
top level: eCFP-GOI
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K678048/annotation2127669/range2127669/1

https://synbiohub.org/public/igem/BBa_K678048/annotation2127669/range2127669/1
type: Range
persistent identity: range2127669
display id: range2127669
version: 1
direction: direction/forward
top level: eCFP-GOI
start: 16
end: 802
orientation: inline

Kosaz
type: SequenceAnnotation
persistent identity: annotation2127793
display id: annotation2127793
version: 1
top level: eCFP-GOI
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678048/annotation2127793/range2127793/1

https://synbiohub.org/public/igem/BBa_K678048/annotation2127793/range2127793/1
type: Range
persistent identity: range2127793
display id: range2127793
version: 1
direction: direction/forward
top level: eCFP-GOI
start: 9
end: 19
orientation: inline

BBa_K1680025
type: ComponentDefinition
persistent identity: BBa K1680025
display id: BBa_K1680025
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RFP-luciferase Cre reporter
creator: Nicolai von Kuegelgen
created: 2015-09-16T11:00:00Z
modified: 2015-10-28T03:54:52Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2098_
m user id: 15693
owner id: 26366
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This part encodes a reporter cassette for the Cre Recombinase (e.g parts XYZ) in Yeast. An active Cre protein will switch expression from RFP to NanoLuc luciferase.
mutable notes: RFC10 assembly
mutable provenance: designed by Team Tuebingen
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_E1010
component: BBa_J63005
component: BBa_K1680005
component: BBa_K1680009
component: BBa_K392003
sequence annotation: BBa_K1680005
sequence annotation: BBa_K1680009
sequence annotation: BBa_K392003
sequence annotation: BBa_J63005
sequence annotation: BBa_E1010
sequence: https://synbiohub.org/public/igem/BBa_K1680025_sequence/1

BBa_E1010
type: Component
persistent identity: component2466050
display id: component2466050
version: 1
top level: BBa_K1680025
access: public
definition: mRFP1

BBa_J63005
type: Component
persistent identity: component2466046
display id: component2466046
version: 1
top level: BBa_K1680025
access: public
definition: https://synbiohub.org/public/igem/BBa_J63005/1

BBa_K1680005
type: Component
persistent identity: component2466052
display id: component2466052
version: 1
top level: BBa_K1680025
access: public
definition: https://synbiohub.org/public/igem/BBa_K1680005/1

BBa_K1680009
type: Component
persistent identity: component2466053
display id: component2466053
version: 1
top level: BBa_K1680025
access: public
definition: https://synbiohub.org/public/igem/BBa_K1680009/1

BBa_K1680005
type: Component
persistent identity: component2466047
display id: component2466047
version: 1
top level: BBa_K1680025
access: public
definition: https://synbiohub.org/public/igem/BBa_K1680005/1

BBa_K392003
type: Component
persistent identity: component2466051
display id: component2466051
version: 1
top level: BBa_K1680025
access: public
definition: https://synbiohub.org/public/igem/BBa_K392003/1

BBa_K1680005
type: SequenceAnnotation
persistent identity: annotation2466047
display id: annotation2466047
version: 1
top level: BBa_K1680025
location: https://synbiohub.org/public/igem/BBa_K1680025/annotation2466047/range2466047/1
component: BBa_K1680005

https://synbiohub.org/public/igem/BBa_K1680025/annotation2466047/range2466047/1
type: Range
persistent identity: range2466047
display id: range2466047
version: 1
direction: direction/reverse
top level: BBa_K1680025
start: 1454
end: 1487
orientation: inline

BBa_K1680005
type: SequenceAnnotation
persistent identity: annotation2466052
display id: annotation2466052
version: 1
top level: BBa_K1680025
location: https://synbiohub.org/public/igem/BBa_K1680025/annotation2466052/range2466052/1
component: BBa_K1680005

https://synbiohub.org/public/igem/BBa_K1680025/annotation2466052/range2466052/1
type: Range
persistent identity: range2466052
display id: range2466052
version: 1
direction: direction/reverse
top level: BBa_K1680025
start: 2345
end: 2378
orientation: inline

BBa_K1680009
type: SequenceAnnotation
persistent identity: annotation2466053
display id: annotation2466053
version: 1
top level: BBa_K1680025
location: https://synbiohub.org/public/igem/BBa_K1680025/annotation2466053/range2466053/1
component: BBa_K1680009

https://synbiohub.org/public/igem/BBa_K1680025/annotation2466053/range2466053/1
type: Range
persistent identity: range2466053
display id: range2466053
version: 1
direction: direction/reverse
top level: BBa_K1680025
start: 2385
end: 2900
orientation: inline

BBa_K392003
type: SequenceAnnotation
persistent identity: annotation2466051
display id: annotation2466051
version: 1
top level: BBa_K1680025
location: https://synbiohub.org/public/igem/BBa_K1680025/annotation2466051/range2466051/1
component: BBa_K392003

https://synbiohub.org/public/igem/BBa_K1680025/annotation2466051/range2466051/1
type: Range
persistent identity: range2466051
display id: range2466051
version: 1
direction: direction/reverse
top level: BBa_K1680025
start: 2208
end: 2336
orientation: inline

BBa_J63005
type: SequenceAnnotation
persistent identity: annotation2466046
display id: annotation2466046
version: 1
top level: BBa_K1680025
location: https://synbiohub.org/public/igem/BBa_K1680025/annotation2466046/range2466046/1
component: BBa_J63005

https://synbiohub.org/public/igem/BBa_K1680025/annotation2466046/range2466046/1
type: Range
persistent identity: range2466046
display id: range2466046
version: 1
direction: direction/reverse
top level: BBa_K1680025
start: 1
end: 1445
orientation: inline

BBa_E1010
type: SequenceAnnotation
persistent identity: annotation2466050
display id: annotation2466050
version: 1
top level: BBa_K1680025
location: https://synbiohub.org/public/igem/BBa_K1680025/annotation2466050/range2466050/1
component: BBa_E1010

https://synbiohub.org/public/igem/BBa_K1680025/annotation2466050/range2466050/1
type: Range
persistent identity: range2466050
display id: range2466050
version: 1
direction: direction/reverse
top level: BBa_K1680025
start: 1494
end: 2199
orientation: inline

BBa_K1031301
type: ComponentDefinition
persistent identity: BBa K1031301
display id: BBa_K1031301
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Pc-B0031-sfGFP-Terminator (HbpR)
creator: He Shuaixin
created: 2013-09-08T11:00:00Z
modified: 2015-05-08T01:08:47Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1338_
m user id: 0
owner id: 15843
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Ph-31-sfGFP-TT (HbpR)
mutable notes: molecular cloning
mutable provenance: synthesis, lab made
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: Ph
sequence annotation: BBa_B0031
sequence annotation: BBa_B0015
sequence annotation: sfGFP
sequence: https://synbiohub.org/public/igem/BBa_K1031301_sequence/1

Ph
type: SequenceAnnotation
persistent identity: annotation2360426
display id: annotation2360426
version: 1
top level: BBa_K1031301
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1031301/annotation2360426/range2360426/1

https://synbiohub.org/public/igem/BBa_K1031301/annotation2360426/range2360426/1
type: Range
persistent identity: range2360426
display id: range2360426
version: 1
direction: direction/forward
top level: BBa_K1031301
start: 1
end: 229
orientation: inline

BBa_B0031
type: SequenceAnnotation
persistent identity: annotation2360427
display id: annotation2360427
version: 1
top level: BBa_K1031301
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1031301/annotation2360427/range2360427/1

https://synbiohub.org/public/igem/BBa_K1031301/annotation2360427/range2360427/1
type: Range
persistent identity: range2360427
display id: range2360427
version: 1
direction: direction/forward
top level: BBa_K1031301
start: 236
end: 248
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2360429
display id: annotation2360429
version: 1
top level: BBa_K1031301
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1031301/annotation2360429/range2360429/1

https://synbiohub.org/public/igem/BBa_K1031301/annotation2360429/range2360429/1
type: Range
persistent identity: range2360429
display id: range2360429
version: 1
direction: direction/forward
top level: BBa_K1031301
start: 984
end: 1112
orientation: inline

sfGFP
type: SequenceAnnotation
persistent identity: annotation2360428
display id: annotation2360428
version: 1
top level: BBa_K1031301
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1031301/annotation2360428/range2360428/1

https://synbiohub.org/public/igem/BBa_K1031301/annotation2360428/range2360428/1
type: Range
persistent identity: range2360428
display id: range2360428
version: 1
direction: direction/forward
top level: BBa_K1031301
start: 255
end: 977
orientation: inline

BBa_K283026
type: ComponentDefinition
persistent identity: BBa K283026
display id: BBa_K283026
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: rbs-GFP(LVA)-terminators (Backbone: pSB1A2)
creator: W Ling , Nelson Chu, Shi Lei
created: 2009-10-12T11:00:00Z
modified: 2015-05-08T01:11:47Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _383_
m user id: 0
owner id: 5494
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: This part is a generator that lacks a promoter. The GFP has a LVA degradation tail. Upon expression, the GFP will give green fluorescence color.
mutable notes: BBa_K283026
mutable provenance: They are from the registry
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0012
component: BBa_B0034
component: BBa_J04031
component: BBa_B0010
sequence annotation: BBa_J04031
sequence annotation: BBa_B0012
sequence annotation: BBa_B0010
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K283026_sequence/1

BBa_B0012
type: Component
persistent identity: component2056834
display id: component2056834
version: 1
top level: BBa_K283026
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0034
type: Component
persistent identity: component2056826
display id: component2056826
version: 1
top level: BBa_K283026
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_J04031
type: Component
persistent identity: component2056831
display id: component2056831
version: 1
top level: BBa_K283026
access: public
definition: https://synbiohub.org/public/igem/BBa_J04031/1

BBa_B0010
type: Component
persistent identity: component2056832
display id: component2056832
version: 1
top level: BBa_K283026
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_J04031
type: SequenceAnnotation
persistent identity: annotation2056831
display id: annotation2056831
version: 1
top level: BBa_K283026
location: https://synbiohub.org/public/igem/BBa_K283026/annotation2056831/range2056831/1
component: BBa_J04031

https://synbiohub.org/public/igem/BBa_K283026/annotation2056831/range2056831/1
type: Range
persistent identity: range2056831
display id: range2056831
version: 1
direction: direction/reverse
top level: BBa_K283026
start: 19
end: 777
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2056834
display id: annotation2056834
version: 1
top level: BBa_K283026
location: https://synbiohub.org/public/igem/BBa_K283026/annotation2056834/range2056834/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K283026/annotation2056834/range2056834/1
type: Range
persistent identity: range2056834
display id: range2056834
version: 1
direction: direction/forward
top level: BBa_K283026
start: 874
end: 914
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2056832
display id: annotation2056832
version: 1
top level: BBa_K283026
location: https://synbiohub.org/public/igem/BBa_K283026/annotation2056832/range2056832/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K283026/annotation2056832/range2056832/1
type: Range
persistent identity: range2056832
display id: range2056832
version: 1
direction: direction/forward
top level: BBa_K283026
start: 786
end: 865
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2056826
display id: annotation2056826
version: 1
top level: BBa_K283026
location: https://synbiohub.org/public/igem/BBa_K283026/annotation2056826/range2056826/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K283026/annotation2056826/range2056826/1
type: Range
persistent identity: range2056826
display id: range2056826
version: 1
direction: direction/reverse
top level: BBa_K283026
start: 1
end: 12
orientation: inline

BBa_K206004
type: ComponentDefinition
persistent identity: BBa K206004
display id: BBa_K206004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RFP generator for testing pBAD wt
creator: Amelia Hardjasa
created: 2009-10-17T11:00:00Z
modified: 2015-05-08T01:11:24Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _307_
m user id: 0
owner id: 4172
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: This is a construct for testing promoter strength by measuring RFP fluorescence.
mutable notes: No special design considerations.
mutable provenance: Assembled from Registry BioBricks.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_I13453
component: BBa_I13507
sequence annotation: BBa_I13453
sequence annotation: BBa_I13507
sequence: https://synbiohub.org/public/igem/BBa_K206004_sequence/1

BBa_I13453
type: Component
persistent identity: component2256290
display id: component2256290
version: 1
top level: BBa_K206004
access: public
definition: https://synbiohub.org/public/igem/BBa_I13453/1

BBa_I13507
type: Component
persistent identity: component2256302
display id: component2256302
version: 1
top level: BBa_K206004
access: public
definition: https://synbiohub.org/public/igem/BBa_I13507/1

BBa_I13453
type: SequenceAnnotation
persistent identity: annotation2256290
display id: annotation2256290
version: 1
top level: BBa_K206004
location: https://synbiohub.org/public/igem/BBa_K206004/annotation2256290/range2256290/1
component: BBa_I13453

https://synbiohub.org/public/igem/BBa_K206004/annotation2256290/range2256290/1
type: Range
persistent identity: range2256290
display id: range2256290
version: 1
direction: direction/reverse
top level: BBa_K206004
start: 1
end: 130
orientation: inline

BBa_I13507
type: SequenceAnnotation
persistent identity: annotation2256302
display id: annotation2256302
version: 1
top level: BBa_K206004
location: https://synbiohub.org/public/igem/BBa_K206004/annotation2256302/range2256302/1
component: BBa_I13507

https://synbiohub.org/public/igem/BBa_K206004/annotation2256302/range2256302/1
type: Range
persistent identity: range2256302
display id: range2256302
version: 1
direction: direction/reverse
top level: BBa_K206004
start: 139
end: 999
orientation: inline

SbpA | CFP
type: ComponentDefinition
persistent identity: BBa K525406
display id: BBa_K525406
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Fusion Protein of S-Layer SbpA and mCerulean
creator: Timo Wolf
created: 2011-09-09T11:00:00Z
modified: 2016-01-26T09:45:16Z
discontinued: false
dominant: false
experience: experience/None
group u list: _690_
m user id: 4206
owner id: 6668
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: Fusion Protein of S-Layer SgsE and mCerulean
mutable notes: Fusion Protein of BBa_K525403 and BBa_J18930
mutable provenance: Fusion Protein of S-Layer SgsE and mCerulean
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: NgoMIV
sequence annotation: mCerulean (CFP)
sequence annotation: ATG
sequence annotation: RBS
sequence annotation: TAA
sequence annotation: Freiburg scar
sequence annotation: AgeI
sequence annotation: J18930
sequence annotation: B0034
sequence annotation: K525401
sequence annotation: SbpA domain
sequence annotation: T7 promotoer
sequence annotation: lac operator
sequence: https://synbiohub.org/public/igem/BBa_K525406_sequence/1

NgoMIV
type: SequenceAnnotation
persistent identity: annotation2129923
display id: annotation2129923
version: 1
top level: SbpA | CFP
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K525406/annotation2129923/range2129923/1

https://synbiohub.org/public/igem/BBa_K525406/annotation2129923/range2129923/1
type: Range
persistent identity: range2129923
display id: range2129923
version: 1
direction: direction/forward
top level: SbpA | CFP
start: 76
end: 81
orientation: inline

mCerulean (CFP)
type: SequenceAnnotation
persistent identity: annotation2129927
display id: annotation2129927
version: 1
top level: SbpA | CFP
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K525406/annotation2129927/range2129927/1

https://synbiohub.org/public/igem/BBa_K525406/annotation2129927/range2129927/1
type: Range
persistent identity: range2129927
display id: range2129927
version: 1
direction: direction/forward
top level: SbpA | CFP
start: 3199
end: 3921
orientation: inline

ATG
type: SequenceAnnotation
persistent identity: annotation2129922
display id: annotation2129922
version: 1
top level: SbpA | CFP
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K525406/annotation2129922/range2129922/1

https://synbiohub.org/public/igem/BBa_K525406/annotation2129922/range2129922/1
type: Range
persistent identity: range2129922
display id: range2129922
version: 1
direction: direction/forward
top level: SbpA | CFP
start: 73
end: 75
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2129919
display id: annotation2129919
version: 1
top level: SbpA | CFP
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K525406/annotation2129919/range2129919/1

https://synbiohub.org/public/igem/BBa_K525406/annotation2129919/range2129919/1
type: Range
persistent identity: range2129919
display id: range2129919
version: 1
direction: direction/forward
top level: SbpA | CFP
start: 56
end: 59
orientation: inline

TAA
type: SequenceAnnotation
persistent identity: annotation2129926
display id: annotation2129926
version: 1
top level: SbpA | CFP
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K525406/annotation2129926/range2129926/1

https://synbiohub.org/public/igem/BBa_K525406/annotation2129926/range2129926/1
type: Range
persistent identity: range2129926
display id: range2129926
version: 1
direction: direction/forward
top level: SbpA | CFP
start: 3919
end: 3921
orientation: inline

Freiburg scar
type: SequenceAnnotation
persistent identity: annotation2129924
display id: annotation2129924
version: 1
top level: SbpA | CFP
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K525406/annotation2129924/range2129924/1

https://synbiohub.org/public/igem/BBa_K525406/annotation2129924/range2129924/1
type: Range
persistent identity: range2129924
display id: range2129924
version: 1
direction: direction/forward
top level: SbpA | CFP
start: 3193
end: 3198
orientation: inline

AgeI
type: SequenceAnnotation
persistent identity: annotation2129928
display id: annotation2129928
version: 1
top level: SbpA | CFP
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K525406/annotation2129928/range2129928/1

https://synbiohub.org/public/igem/BBa_K525406/annotation2129928/range2129928/1
type: Range
persistent identity: range2129928
display id: range2129928
version: 1
direction: direction/forward
top level: SbpA | CFP
start: 3913
end: 3918
orientation: inline

J18930
type: SequenceAnnotation
persistent identity: annotation2129925
display id: annotation2129925
version: 1
top level: SbpA | CFP
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K525406/annotation2129925/range2129925/1

https://synbiohub.org/public/igem/BBa_K525406/annotation2129925/range2129925/1
type: Range
persistent identity: range2129925
display id: range2129925
version: 1
direction: direction/forward
top level: SbpA | CFP
start: 3199
end: 3921
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation2129918
display id: annotation2129918
version: 1
top level: SbpA | CFP
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K525406/annotation2129918/range2129918/1

https://synbiohub.org/public/igem/BBa_K525406/annotation2129918/range2129918/1
type: Range
persistent identity: range2129918
display id: range2129918
version: 1
direction: direction/forward
top level: SbpA | CFP
start: 53
end: 64
orientation: inline

K525401
type: SequenceAnnotation
persistent identity: annotation2129920
display id: annotation2129920
version: 1
top level: SbpA | CFP
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K525406/annotation2129920/range2129920/1

https://synbiohub.org/public/igem/BBa_K525406/annotation2129920/range2129920/1
type: Range
persistent identity: range2129920
display id: range2129920
version: 1
direction: direction/forward
top level: SbpA | CFP
start: 73
end: 3192
orientation: inline

SbpA domain
type: SequenceAnnotation
persistent identity: annotation2129921
display id: annotation2129921
version: 1
top level: SbpA | CFP
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K525406/annotation2129921/range2129921/1

https://synbiohub.org/public/igem/BBa_K525406/annotation2129921/range2129921/1
type: Range
persistent identity: range2129921
display id: range2129921
version: 1
direction: direction/forward
top level: SbpA | CFP
start: 73
end: 3192
orientation: inline

T7 promotoer
type: SequenceAnnotation
persistent identity: annotation2129916
display id: annotation2129916
version: 1
top level: SbpA | CFP
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K525406/annotation2129916/range2129916/1

https://synbiohub.org/public/igem/BBa_K525406/annotation2129916/range2129916/1
type: Range
persistent identity: range2129916
display id: range2129916
version: 1
direction: direction/forward
top level: SbpA | CFP
start: 1
end: 20
orientation: inline

lac operator
type: SequenceAnnotation
persistent identity: annotation2129917
display id: annotation2129917
version: 1
top level: SbpA | CFP
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K525406/annotation2129917/range2129917/1

https://synbiohub.org/public/igem/BBa_K525406/annotation2129917/range2129917/1
type: Range
persistent identity: range2129917
display id: range2129917
version: 1
direction: direction/forward
top level: SbpA | CFP
start: 20
end: 44
orientation: inline

BBa_K1399003
type: ComponentDefinition
persistent identity: BBa K1399003
display id: BBa_K1399003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RFP from Discosoma striata (coral) with DAS-ssrA degradation tag
creator: Anna Stikane
created: 2014-09-18T11:00:00Z
modified: 2015-05-08T01:10:16Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1777_
m user id: 0
owner id: 22477
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: Mutant RFP from Discosoma striata (coral) (see part BBa_E1010) with added DAS-ssrA degradation tag (part BBa_M0052). The tag increases RFP turn-over rate, thus providing better temporal resolution of red fluorescence. In the same time, maximal fluorescence amplitudes will be lower as newly formed protein is degraded as soon as it is formed. The tag encodes peptide sequence AANDENYADAS and is recognized by ClpA and ClpX unfoldases and ClpX mediator SspB.[1] ClpA and ClpX then form a proteosome-like complex with ClpP protease and the protein is degraded.[1] The final three residues of the tag determines the strength of interaction with ClpX and thus the final protein degradation rate.[2] The DAS tag is reported to have low affinity to ClpX thus its mediated degradation very much depends on the concentration of SspB (ClpX mediator).[1] However, be aware that exact protein degradation rate is influenced by multiple other factors: ClpXP and ClpAP protease concentrations, protein stability, Km of binding to the protease, temperature [3]. References: [1] Flynn, J. M. et al. Overlapping recognition determinants within the ssrA degradation tag allow modulation of proteolysis. Proc. Natl. Acad. Sci. U. S. A. 98, 10584???9 (2001). [2] Andersen, J. B. et al. New unstable variants of green fluorescent protein for studies of transient gene expression in bacteria. Appl. Environ. Microbiol. 64, 2240???6 (1998). [3] Purcell, O., Grierson, C. S., Bernardo, M. Di & Savery, N. J. Temperature dependence of ssrA-tag mediated protein degradation. J. Biol. Eng. 6, 10 (2012).
mutable notes: The tag was attached to RFP using PCR and MABEL (mutagenesis with blunt-end ligation), thus avoiding introduction of additonal residues and restriction site. Different parts of the tag are recognized by different proteins, for example, the final 3 residues (AAV in this case) are recognised by ClpX, whereas first 4 residues of the tag are required for efficient SspB binding.[1] Thus modifications of these critical residues alter the efficacy with what different proteases bind to it.
mutable provenance: RFP comes from part BBa_E1010, tag sequence was obtained from part BBa_M0052, but same tag was also used in paper by Andersen et al., (1998).[2]
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: start
sequence annotation: RFP
sequence annotation: stop
sequence annotation: DAS-ssrA tag
sequence: https://synbiohub.org/public/igem/BBa_K1399003_sequence/1

start
type: SequenceAnnotation
persistent identity: annotation2383903
display id: annotation2383903
version: 1
top level: BBa_K1399003
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1399003/annotation2383903/range2383903/1

https://synbiohub.org/public/igem/BBa_K1399003/annotation2383903/range2383903/1
type: Range
persistent identity: range2383903
display id: range2383903
version: 1
direction: direction/forward
top level: BBa_K1399003
start: 1
end: 3
orientation: inline

RFP
type: SequenceAnnotation
persistent identity: annotation2383904
display id: annotation2383904
version: 1
top level: BBa_K1399003
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1399003/annotation2383904/range2383904/1

https://synbiohub.org/public/igem/BBa_K1399003/annotation2383904/range2383904/1
type: Range
persistent identity: range2383904
display id: range2383904
version: 1
direction: direction/forward
top level: BBa_K1399003
start: 4
end: 675
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2383906
display id: annotation2383906
version: 1
top level: BBa_K1399003
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1399003/annotation2383906/range2383906/1

https://synbiohub.org/public/igem/BBa_K1399003/annotation2383906/range2383906/1
type: Range
persistent identity: range2383906
display id: range2383906
version: 1
direction: direction/forward
top level: BBa_K1399003
start: 709
end: 711
orientation: inline

DAS-ssrA tag
type: SequenceAnnotation
persistent identity: annotation2383905
display id: annotation2383905
version: 1
top level: BBa_K1399003
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1399003/annotation2383905/range2383905/1

https://synbiohub.org/public/igem/BBa_K1399003/annotation2383905/range2383905/1
type: Range
persistent identity: range2383905
display id: range2383905
version: 1
direction: direction/forward
top level: BBa_K1399003
start: 676
end: 708
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2383907
display id: annotation2383907
version: 1
top level: BBa_K1399003
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1399003/annotation2383907/range2383907/1

https://synbiohub.org/public/igem/BBa_K1399003/annotation2383907/range2383907/1
type: Range
persistent identity: range2383907
display id: range2383907
version: 1
direction: direction/forward
top level: BBa_K1399003
start: 712
end: 714
orientation: inline

BBa_K1033916
type: ComponentDefinition
persistent identity: BBa K1033916
display id: BBa_K1033916
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: amajLime, yellow-green chromoprotein
creator: Sabri Jamal
created: 2013-08-24T11:00:00Z
modified: 2015-05-08T01:08:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1340_
m user id: 0
owner id: 10137
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This chromoprotein from the coral Anemonia majano, amajLime, naturally exhibits strong color when expressed.
mutable notes: Synthesized and codon optimized for E coli by GenScript.
mutable provenance: The anemone Anemonia majano.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: amajLime
sequence: https://synbiohub.org/public/igem/BBa_K1033916_sequence/1

amajLime
type: SequenceAnnotation
persistent identity: annotation2332103
display id: annotation2332103
version: 1
top level: BBa_K1033916
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1033916/annotation2332103/range2332103/1

https://synbiohub.org/public/igem/BBa_K1033916/annotation2332103/range2332103/1
type: Range
persistent identity: range2332103
display id: range2332103
version: 1
direction: direction/forward
top level: BBa_K1033916
start: 1
end: 690
orientation: inline

mRFP1
type: ComponentDefinition
persistent identity: BBa E1010
display id: BBa_E1010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: **highly** engineered mutant of red fluorescent protein from Discosoma striata (coral)
creator: Drew Endy
created: 2004-07-27T11:00:00Z
modified: 2015-08-31T04:07:26Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _11_1_
m user id: 0
owner id: 52
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: monomeric RFP: Red Fluorescent Protein. Excitation peak: 584 nm Emission peak: 607 nm
mutable notes: TAATAA double stop codon added (DE). Four silent mutations made to remove three EcoRI sites and one PstI site: A28G, A76G, A349G, G337A.
mutable provenance: Campbell et al., PNAS v99 p7877 URL
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: mrfp1
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_E1010_sequence/1

mrfp1
type: SequenceAnnotation
persistent identity: annotation1014044
display id: annotation1014044
version: 1
top level: mRFP1
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_E1010/annotation1014044/range1014044/1

https://synbiohub.org/public/igem/BBa_E1010/annotation1014044/range1014044/1
type: Range
persistent identity: range1014044
display id: range1014044
version: 1
direction: direction/forward
top level: mRFP1
start: 1
end: 675
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2214014
display id: annotation2214014
version: 1
top level: mRFP1
role: SO:0000807
role: feature/barcode
location: https://synbiohub.org/public/igem/BBa_E1010/annotation2214014/range2214014/1

https://synbiohub.org/public/igem/BBa_E1010/annotation2214014/range2214014/1
type: Range
persistent identity: range2214014
display id: range2214014
version: 1
direction: direction/forward
top level: mRFP1
start: 682
end: 706
orientation: inline

BBa_K1486013
type: ComponentDefinition
persistent identity: BBa K1486013
display id: BBa_K1486013
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CpxR-IFP[2] under pBAD
creator: iGem EPFL 2014
created: 2014-08-31T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part has a fused protein downstream of an arabinose-inducible promoter: CpxR-IFP[2]. Biobrick BBa_K1486012 has CpxR-IFP[1] with an arabinose promoter. This construct was made in both orientations: BBa_K1486015 is IFP[2]-CpxR (with an arabinose promoter).
mutable notes: Both orientations were made, as well as for IFP[1] so all dimerizations could be tested (see main page for biobrick numbers). Flexible linkers were used to fuse both proteins, and an RBS was put before each one of them.
mutable provenance: to edit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K1486000
component: BBa_K1486004
component: BBa_K1486007
component: BBa_B0034
component: BBa_I0500
sequence annotation: BBa_B0034
sequence annotation: BBa_K1486000
sequence annotation: BBa_I0500
sequence annotation: BBa_K1486007
sequence annotation: BBa_K1486004
sequence: https://synbiohub.org/public/igem/BBa_K1486013_sequence/1

BBa_K1486000
type: Component
persistent identity: component2382303
display id: component2382303
version: 1
top level: BBa_K1486013
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486000/1

BBa_K1486004
type: Component
persistent identity: component2382304
display id: component2382304
version: 1
top level: BBa_K1486013
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486004/1

BBa_K1486007
type: Component
persistent identity: component2382308
display id: component2382308
version: 1
top level: BBa_K1486013
access: public
definition: BBa_K1486007

BBa_B0034
type: Component
persistent identity: component2382299
display id: component2382299
version: 1
top level: BBa_K1486013
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_I0500
type: Component
persistent identity: component2382297
display id: component2382297
version: 1
top level: BBa_K1486013
access: public
definition: https://synbiohub.org/public/igem/BBa_I0500/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2382299
display id: annotation2382299
version: 1
top level: BBa_K1486013
location: https://synbiohub.org/public/igem/BBa_K1486013/annotation2382299/range2382299/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1486013/annotation2382299/range2382299/1
type: Range
persistent identity: range2382299
display id: range2382299
version: 1
direction: direction/reverse
top level: BBa_K1486013
start: 1211
end: 1222
orientation: inline

BBa_K1486000
type: SequenceAnnotation
persistent identity: annotation2382303
display id: annotation2382303
version: 1
top level: BBa_K1486013
location: https://synbiohub.org/public/igem/BBa_K1486013/annotation2382303/range2382303/1
component: BBa_K1486000

https://synbiohub.org/public/igem/BBa_K1486013/annotation2382303/range2382303/1
type: Range
persistent identity: range2382303
display id: range2382303
version: 1
direction: direction/reverse
top level: BBa_K1486013
start: 1223
end: 1924
orientation: inline

BBa_I0500
type: SequenceAnnotation
persistent identity: annotation2382297
display id: annotation2382297
version: 1
top level: BBa_K1486013
location: https://synbiohub.org/public/igem/BBa_K1486013/annotation2382297/range2382297/1
component: BBa_I0500

https://synbiohub.org/public/igem/BBa_K1486013/annotation2382297/range2382297/1
type: Range
persistent identity: range2382297
display id: range2382297
version: 1
direction: direction/reverse
top level: BBa_K1486013
start: 1
end: 1210
orientation: inline

BBa_K1486007
type: SequenceAnnotation
persistent identity: annotation2382308
display id: annotation2382308
version: 1
top level: BBa_K1486013
location: https://synbiohub.org/public/igem/BBa_K1486013/annotation2382308/range2382308/1
component: BBa_K1486007

https://synbiohub.org/public/igem/BBa_K1486013/annotation2382308/range2382308/1
type: Range
persistent identity: range2382308
display id: range2382308
version: 1
direction: direction/reverse
top level: BBa_K1486013
start: 1955
end: 2533
orientation: inline

BBa_K1486004
type: SequenceAnnotation
persistent identity: annotation2382304
display id: annotation2382304
version: 1
top level: BBa_K1486013
location: https://synbiohub.org/public/igem/BBa_K1486013/annotation2382304/range2382304/1
component: BBa_K1486004

https://synbiohub.org/public/igem/BBa_K1486013/annotation2382304/range2382304/1
type: Range
persistent identity: range2382304
display id: range2382304
version: 1
direction: direction/reverse
top level: BBa_K1486013
start: 1925
end: 1954
orientation: inline

BBa_K1680024
type: ComponentDefinition
persistent identity: BBa K1680024
display id: BBa_K1680024
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Dronpa caged Cre with NLS
creator: Nicolai von Kuegelgen
created: 2015-09-16T11:00:00Z
modified: 2016-01-26T10:22:01Z
discontinued: false
dominant: false
group u list: _2098_
m user id: 4206
owner id: 26366
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Protein coding region for a fusion protein of Cre recombinase (BBa_K1680007) that is C- & N-terminal flanked with 145N Dronpa (BBa_K1680006) using a 15 AA linker as spacer (BBa_K1680003). The construct has a N-terminal NLS sequence (BBa_K1680004). In the expressed construct the two dronpa domains should form multimerize leading to an inactive Cre recombinase that is reversibly activatable through dronpa illumination (see Zhou et al 2012).
mutable notes: 3A assembly with RFC25
mutable provenance: gBlock design by Team Tuebingen
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: K1680003
sequence annotation: K1680006
sequence annotation: K1680007
sequence annotation: K1680004
sequence: https://synbiohub.org/public/igem/BBa_K1680024_sequence/1

K1680003
type: SequenceAnnotation
persistent identity: annotation2477396
display id: annotation2477396
version: 1
top level: BBa_K1680024
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1680024/annotation2477396/range2477396/1

https://synbiohub.org/public/igem/BBa_K1680024/annotation2477396/range2477396/1
type: Range
persistent identity: range2477396
display id: range2477396
version: 1
direction: direction/forward
top level: BBa_K1680024
start: 1774
end: 1817
orientation: inline

K1680003
type: SequenceAnnotation
persistent identity: annotation2477395
display id: annotation2477395
version: 1
top level: BBa_K1680024
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1680024/annotation2477395/range2477395/1

https://synbiohub.org/public/igem/BBa_K1680024/annotation2477395/range2477395/1
type: Range
persistent identity: range2477395
display id: range2477395
version: 1
direction: direction/forward
top level: BBa_K1680024
start: 700
end: 744
orientation: inline

K1680006
type: SequenceAnnotation
persistent identity: annotation2477394
display id: annotation2477394
version: 1
top level: BBa_K1680024
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1680024/annotation2477394/range2477394/1

https://synbiohub.org/public/igem/BBa_K1680024/annotation2477394/range2477394/1
type: Range
persistent identity: range2477394
display id: range2477394
version: 1
direction: direction/forward
top level: BBa_K1680024
start: 1818
end: 2487
orientation: inline

K1680007
type: SequenceAnnotation
persistent identity: annotation2477392
display id: annotation2477392
version: 1
top level: BBa_K1680024
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1680024/annotation2477392/range2477392/1

https://synbiohub.org/public/igem/BBa_K1680024/annotation2477392/range2477392/1
type: Range
persistent identity: range2477392
display id: range2477392
version: 1
direction: direction/forward
top level: BBa_K1680024
start: 745
end: 1773
orientation: inline

K1680006
type: SequenceAnnotation
persistent identity: annotation2477393
display id: annotation2477393
version: 1
top level: BBa_K1680024
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1680024/annotation2477393/range2477393/1

https://synbiohub.org/public/igem/BBa_K1680024/annotation2477393/range2477393/1
type: Range
persistent identity: range2477393
display id: range2477393
version: 1
direction: direction/forward
top level: BBa_K1680024
start: 31
end: 699
orientation: inline

K1680004
type: SequenceAnnotation
persistent identity: annotation2477390
display id: annotation2477390
version: 1
top level: BBa_K1680024
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1680024/annotation2477390/range2477390/1

https://synbiohub.org/public/igem/BBa_K1680024/annotation2477390/range2477390/1
type: Range
persistent identity: range2477390
display id: range2477390
version: 1
direction: direction/forward
top level: BBa_K1680024
start: 1
end: 24
orientation: inline

BBa_K1031224
type: ComponentDefinition
persistent identity: BBa K1031224
display id: BBa_K1031224
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Po-B0034-sfGFP-Terminator (DmpR)
creator: He Shuaixin
created: 2013-09-08T11:00:00Z
modified: 2015-05-08T01:08:47Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1338_
m user id: 0
owner id: 15843
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Po-34-sfGFP-TT (DmpR)
mutable notes: molecular cloning
mutable provenance: synthesis, lab made
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: po promoter
sequence annotation: B0015
sequence annotation: B0034
sequence annotation: sfGFP
sequence: https://synbiohub.org/public/igem/BBa_K1031224_sequence/1

po promoter
type: SequenceAnnotation
persistent identity: annotation2354390
display id: annotation2354390
version: 1
top level: BBa_K1031224
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1031224/annotation2354390/range2354390/1

https://synbiohub.org/public/igem/BBa_K1031224/annotation2354390/range2354390/1
type: Range
persistent identity: range2354390
display id: range2354390
version: 1
direction: direction/forward
top level: BBa_K1031224
start: 1
end: 171
orientation: inline

B0015
type: SequenceAnnotation
persistent identity: annotation2354393
display id: annotation2354393
version: 1
top level: BBa_K1031224
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1031224/annotation2354393/range2354393/1

https://synbiohub.org/public/igem/BBa_K1031224/annotation2354393/range2354393/1
type: Range
persistent identity: range2354393
display id: range2354393
version: 1
direction: direction/forward
top level: BBa_K1031224
start: 929
end: 1057
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation2354391
display id: annotation2354391
version: 1
top level: BBa_K1031224
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1031224/annotation2354391/range2354391/1

https://synbiohub.org/public/igem/BBa_K1031224/annotation2354391/range2354391/1
type: Range
persistent identity: range2354391
display id: range2354391
version: 1
direction: direction/forward
top level: BBa_K1031224
start: 180
end: 191
orientation: inline

sfGFP
type: SequenceAnnotation
persistent identity: annotation2354392
display id: annotation2354392
version: 1
top level: BBa_K1031224
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1031224/annotation2354392/range2354392/1

https://synbiohub.org/public/igem/BBa_K1031224/annotation2354392/range2354392/1
type: Range
persistent identity: range2354392
display id: range2354392
version: 1
direction: direction/forward
top level: BBa_K1031224
start: 198
end: 920
orientation: inline

eGFP
type: ComponentDefinition
persistent identity: BBa K678006
display id: BBa_K678006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: eGFP for mammalian cells
creator: DTU-Denmark-2
created: 2011-09-03T11:00:00Z
modified: 2015-05-08T01:13:04Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _882_
m user id: 0
owner id: 8341
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: enhanced green fluorescent protein for use in mammalian cells
mutable notes: Compatible with the Plug'n'Play assembly standard
mutable provenance: plasmid pEGFP-1 from Clontech
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: eGFP
sequence annotation: Kozak
sequence annotation: Linker 2
sequence: https://synbiohub.org/public/igem/BBa_K678006_sequence/1

eGFP
type: SequenceAnnotation
persistent identity: annotation2127349
display id: annotation2127349
version: 1
top level: eGFP
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K678006/annotation2127349/range2127349/1

https://synbiohub.org/public/igem/BBa_K678006/annotation2127349/range2127349/1
type: Range
persistent identity: range2127349
display id: range2127349
version: 1
direction: direction/forward
top level: eGFP
start: 16
end: 735
orientation: inline

Kozak
type: SequenceAnnotation
persistent identity: annotation2127774
display id: annotation2127774
version: 1
top level: eGFP
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678006/annotation2127774/range2127774/1

https://synbiohub.org/public/igem/BBa_K678006/annotation2127774/range2127774/1
type: Range
persistent identity: range2127774
display id: range2127774
version: 1
direction: direction/forward
top level: eGFP
start: 9
end: 19
orientation: inline

Linker 2
type: SequenceAnnotation
persistent identity: annotation2127348
display id: annotation2127348
version: 1
top level: eGFP
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678006/annotation2127348/range2127348/1

https://synbiohub.org/public/igem/BBa_K678006/annotation2127348/range2127348/1
type: Range
persistent identity: range2127348
display id: range2127348
version: 1
direction: direction/forward
top level: eGFP
start: 1
end: 8
orientation: inline

BBa_K1399018
type: ComponentDefinition
persistent identity: BBa K1399018
display id: BBa_K1399018
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Plac-GFP(DAS)
creator: Anna Stikane
created: 2014-09-18T11:00:00Z
modified: 2015-05-08T01:10:16Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1777_
m user id: 0
owner id: 22477
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: Lactose/IPTG inducible promoter with GFP reporter tagged with DAS-ssrA degradation tag followed by terminator. The tagged GFP is actively degraded within cell, thus provides better temporal resolution of green fluorescence and promoter activity. Presence of SspB is crucial for effective protein degradation.
mutable notes: Part was assembled using BrickClip assembly (BBF RFC104) using other biobrick parts as templates. BrickClip assembly is a special case of more general Paperclip assembly method.[1]
mutable provenance: other biobricks
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_R0010
component: BBa_K1399008
component: BBa_B0034
component: BBa_B0015
sequence annotation: BBa_B0034
sequence annotation: BBa_R0010
sequence annotation: BBa_K1399008
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_K1399018_sequence/1

BBa_R0010
type: Component
persistent identity: component2384197
display id: component2384197
version: 1
top level: BBa_K1399018
access: public
definition: https://synbiohub.org/public/igem/BBa_R0010/1

BBa_K1399008
type: Component
persistent identity: component2384211
display id: component2384211
version: 1
top level: BBa_K1399018
access: public
definition: BBa_K1399008

BBa_B0034
type: Component
persistent identity: component2384205
display id: component2384205
version: 1
top level: BBa_K1399018
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0015
type: Component
persistent identity: component2384218
display id: component2384218
version: 1
top level: BBa_K1399018
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2384205
display id: annotation2384205
version: 1
top level: BBa_K1399018
location: https://synbiohub.org/public/igem/BBa_K1399018/annotation2384205/range2384205/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1399018/annotation2384205/range2384205/1
type: Range
persistent identity: range2384205
display id: range2384205
version: 1
direction: direction/reverse
top level: BBa_K1399018
start: 209
end: 220
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation2384197
display id: annotation2384197
version: 1
top level: BBa_K1399018
location: https://synbiohub.org/public/igem/BBa_K1399018/annotation2384197/range2384197/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_K1399018/annotation2384197/range2384197/1
type: Range
persistent identity: range2384197
display id: range2384197
version: 1
direction: direction/forward
top level: BBa_K1399018
start: 1
end: 200
orientation: inline

BBa_K1399008
type: SequenceAnnotation
persistent identity: annotation2384211
display id: annotation2384211
version: 1
top level: BBa_K1399018
location: https://synbiohub.org/public/igem/BBa_K1399018/annotation2384211/range2384211/1
component: BBa_K1399008

https://synbiohub.org/public/igem/BBa_K1399018/annotation2384211/range2384211/1
type: Range
persistent identity: range2384211
display id: range2384211
version: 1
direction: direction/reverse
top level: BBa_K1399018
start: 227
end: 979
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2384218
display id: annotation2384218
version: 1
top level: BBa_K1399018
location: https://synbiohub.org/public/igem/BBa_K1399018/annotation2384218/range2384218/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K1399018/annotation2384218/range2384218/1
type: Range
persistent identity: range2384218
display id: range2384218
version: 1
direction: direction/reverse
top level: BBa_K1399018
start: 988
end: 1116
orientation: inline

BBa_K1742002
type: ComponentDefinition
persistent identity: BBa K1742002
display id: BBa_K1742002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Dronpa 145N
creator: Iv??n Casas Rodrigo
created: 2015-09-03T11:00:00Z
modified: 2016-01-25T11:27:00Z
discontinued: false
dominant: false
group u list: _2164_
m user id: 4206
owner id: 26598
owning group id: 9
status: status/Planning
bookmark: false
mutable description: A CDS of Dronpa protein with a mutation in the betha-strand 7 in Lys145 for Asn.
mutable notes: It is codon optimized for plants
mutable provenance: Pectinidae sp. sequence found in the following article Lummer, M., Humpert, F., Steuwe, C., Caesar, K., Sch??ttpelz, M., Sauer, M. and Staiger, D. (2011), Reversible Photoswitchable DRONPA-s Monitors Nucleocytoplasmic Transport of an RNA-Binding Protein in Transgenic Plants. Traffic, 12: 693???702. doi: 10.1111/j.1600-0854.2011.01180.x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1742002_sequence/1

BBa_K678016
type: ComponentDefinition
persistent identity: BBa K678016
display id: BBa_K678016
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: eCFP-TS
creator: DTU-Denmark-2
created: 2011-09-12T11:00:00Z
modified: 2015-05-08T01:13:04Z
discontinued: false
dominant: false
experience: experience/None
group u list: _882_
m user id: 0
owner id: 10556
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part was designed for the Plug'n'Play with DNA assembly standard (RFC 80), therefore this part contains tails at the 5' and 3' ends as shown on the figure below.
mutable notes: No
mutable provenance: The Danish Cancer Society
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: eCFP-TS
sequence annotation: Kozak
sequence annotation: Linker 3
sequence: https://synbiohub.org/public/igem/BBa_K678016_sequence/1

eCFP-TS
type: SequenceAnnotation
persistent identity: annotation2127659
display id: annotation2127659
version: 1
top level: BBa_K678016
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K678016/annotation2127659/range2127659/1

https://synbiohub.org/public/igem/BBa_K678016/annotation2127659/range2127659/1
type: Range
persistent identity: range2127659
display id: range2127659
version: 1
direction: direction/forward
top level: BBa_K678016
start: 17
end: 814
orientation: inline

Kozak
type: SequenceAnnotation
persistent identity: annotation2127794
display id: annotation2127794
version: 1
top level: BBa_K678016
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678016/annotation2127794/range2127794/1

https://synbiohub.org/public/igem/BBa_K678016/annotation2127794/range2127794/1
type: Range
persistent identity: range2127794
display id: range2127794
version: 1
direction: direction/forward
top level: BBa_K678016
start: 10
end: 20
orientation: inline

Linker 3
type: SequenceAnnotation
persistent identity: annotation2127653
display id: annotation2127653
version: 1
top level: BBa_K678016
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678016/annotation2127653/range2127653/1

https://synbiohub.org/public/igem/BBa_K678016/annotation2127653/range2127653/1
type: Range
persistent identity: range2127653
display id: range2127653
version: 1
direction: direction/forward
top level: BBa_K678016
start: 1
end: 9
orientation: inline

pBAD-GFP-c
type: ComponentDefinition
persistent identity: BBa K845009
display id: BBa_K845009
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: paraBAD_gfp_cyaA
creator: Gr??gory Hansen
created: 2012-09-25T11:00:00Z
modified: 2015-05-08T01:13:33Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1105_
m user id: 0
owner id: 12465
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: ...
mutable notes: ...
mutable provenance: ...
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K845000
component: BBa_K845008
sequence annotation: BBa_K845008
sequence annotation: BBa_K845000
sequence: https://synbiohub.org/public/igem/BBa_K845009_sequence/1

BBa_K845000
type: Component
persistent identity: component2204289
display id: component2204289
version: 1
top level: pBAD-GFP-c
access: public
definition: pBAD-GFP

BBa_K845008
type: Component
persistent identity: component2204292
display id: component2204292
version: 1
top level: pBAD-GFP-c
access: public
definition: https://synbiohub.org/public/igem/BBa_K845008/1

BBa_K845008
type: SequenceAnnotation
persistent identity: annotation2204292
display id: annotation2204292
version: 1
top level: pBAD-GFP-c
location: https://synbiohub.org/public/igem/BBa_K845009/annotation2204292/range2204292/1
component: BBa_K845008

https://synbiohub.org/public/igem/BBa_K845009/annotation2204292/range2204292/1
type: Range
persistent identity: range2204292
display id: range2204292
version: 1
direction: direction/reverse
top level: pBAD-GFP-c
start: 1251
end: 3827
orientation: inline

BBa_K845000
type: SequenceAnnotation
persistent identity: annotation2204289
display id: annotation2204289
version: 1
top level: pBAD-GFP-c
location: https://synbiohub.org/public/igem/BBa_K845009/annotation2204289/range2204289/1
component: BBa_K845000

https://synbiohub.org/public/igem/BBa_K845009/annotation2204289/range2204289/1
type: Range
persistent identity: range2204289
display id: range2204289
version: 1
direction: direction/reverse
top level: pBAD-GFP-c
start: 1
end: 1242
orientation: inline

BBa_K1031113
type: ComponentDefinition
persistent identity: BBa K1031113
display id: BBa_K1031113
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Po-B0031-sfGFP-Terminator (CapR)
creator: He Shuaixin
created: 2013-09-08T11:00:00Z
modified: 2015-05-08T01:08:47Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1338_
m user id: 0
owner id: 15843
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Po-31-sfGFP-TT (CapR)
mutable notes: molecular cloning
mutable provenance: synthesis
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: Po (CapR)
sequence annotation: B0031
sequence annotation: B0015
sequence annotation: sfGFP
sequence: https://synbiohub.org/public/igem/BBa_K1031113_sequence/1

Po (CapR)
type: SequenceAnnotation
persistent identity: annotation2362064
display id: annotation2362064
version: 1
top level: BBa_K1031113
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1031113/annotation2362064/range2362064/1

https://synbiohub.org/public/igem/BBa_K1031113/annotation2362064/range2362064/1
type: Range
persistent identity: range2362064
display id: range2362064
version: 1
direction: direction/forward
top level: BBa_K1031113
start: 1
end: 170
orientation: inline

B0031
type: SequenceAnnotation
persistent identity: annotation2362065
display id: annotation2362065
version: 1
top level: BBa_K1031113
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1031113/annotation2362065/range2362065/1

https://synbiohub.org/public/igem/BBa_K1031113/annotation2362065/range2362065/1
type: Range
persistent identity: range2362065
display id: range2362065
version: 1
direction: direction/forward
top level: BBa_K1031113
start: 177
end: 189
orientation: inline

B0015
type: SequenceAnnotation
persistent identity: annotation2362067
display id: annotation2362067
version: 1
top level: BBa_K1031113
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1031113/annotation2362067/range2362067/1

https://synbiohub.org/public/igem/BBa_K1031113/annotation2362067/range2362067/1
type: Range
persistent identity: range2362067
display id: range2362067
version: 1
direction: direction/forward
top level: BBa_K1031113
start: 925
end: 1054
orientation: inline

sfGFP
type: SequenceAnnotation
persistent identity: annotation2362066
display id: annotation2362066
version: 1
top level: BBa_K1031113
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1031113/annotation2362066/range2362066/1

https://synbiohub.org/public/igem/BBa_K1031113/annotation2362066/range2362066/1
type: Range
persistent identity: range2362066
display id: range2362066
version: 1
direction: direction/forward
top level: BBa_K1031113
start: 197
end: 919
orientation: inline

BBa_K1399008
type: ComponentDefinition
persistent identity: BBa K1399008
display id: BBa_K1399008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: GFP (mut3b) with DAS-ssrA degradation tag
creator: Anna Stikane
created: 2014-09-18T11:00:00Z
modified: 2015-05-08T01:10:16Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1777_
m user id: 0
owner id: 22477
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: GFP (mut3b) (see part BBa_E0040) with added DAS-ssrA degradation tag (see part BBa_M0052). The tag increases GFP turn-over rate, thus providing better temporal resolution of green fluorescence. In the same time, maximal fluorescence amplitudes will be lower as newly formed protein is degraded as soon as it is formed. SsrA tags encode peptide sequence that is recognized by ClpA and ClpX unfoldases and ClpX mediator SspB.[1] ClpA and ClpX then form a proteosome-like complex with ClpP protease and the protein is degraded.[1] The final three residues of the tag determines the strength of interaction with ClpX and thus the final protein degradation rate.[2] The DAS tag encodes peptide sequence AANDENYADAS is reported to have low affinity to ClpX thus its mediated degradation very much depends on the concentration of SspB (ClpX mediator).[1] However, be aware that exact protein degradation rate is influenced by multiple other factors: ClpXP and ClpAP protease concentrations, protein stability, Km of binding to the protease, temperature [3]. ===References=== [1] Flynn, J. M. et al. Overlapping recognition determinants within the ssrA degradation tag allow modulation of proteolysis. Proc. Natl. Acad. Sci. U. S. A. 98, 10584???9 (2001). [2] Andersen, J. B. et al. New unstable variants of green fluorescent protein for studies of transient gene expression in bacteria. Appl. Environ. Microbiol. 64, 2240???6 (1998). [3] Purcell, O., Grierson, C. S., Bernardo, M. Di & Savery, N. J. Temperature dependence of ssrA-tag mediated protein degradation. J. Biol. Eng. 6, 10 (2012).
mutable notes: The tag was attached to GFP using PCR and MABEL (mutagenesis with blunt-end ligation), thus avoiding introduction of additional residues and restriction site. Different parts of the tag are recognized by different proteins, for example, the final 3 residues (DAS in this case) are recognized by ClpX, whereas first 4 residues of the tag are required for efficient SspB binding.[1] Thus modifications of these critical residues alter the efficacy with what different proteases bind to it.
mutable provenance: GFP comes from part BBa_E0040, the tag sequence was obtained from part BBa_M0052.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: start
sequence annotation: stop
sequence annotation: DAS-ssrA tag
sequence annotation: GFP (mut3b)
sequence: https://synbiohub.org/public/igem/BBa_K1399008_sequence/1

start
type: SequenceAnnotation
persistent identity: annotation2383928
display id: annotation2383928
version: 1
top level: BBa_K1399008
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1399008/annotation2383928/range2383928/1

https://synbiohub.org/public/igem/BBa_K1399008/annotation2383928/range2383928/1
type: Range
persistent identity: range2383928
display id: range2383928
version: 1
direction: direction/forward
top level: BBa_K1399008
start: 1
end: 3
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2383932
display id: annotation2383932
version: 1
top level: BBa_K1399008
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1399008/annotation2383932/range2383932/1

https://synbiohub.org/public/igem/BBa_K1399008/annotation2383932/range2383932/1
type: Range
persistent identity: range2383932
display id: range2383932
version: 1
direction: direction/forward
top level: BBa_K1399008
start: 751
end: 753
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2383931
display id: annotation2383931
version: 1
top level: BBa_K1399008
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1399008/annotation2383931/range2383931/1

https://synbiohub.org/public/igem/BBa_K1399008/annotation2383931/range2383931/1
type: Range
persistent identity: range2383931
display id: range2383931
version: 1
direction: direction/forward
top level: BBa_K1399008
start: 748
end: 750
orientation: inline

DAS-ssrA tag
type: SequenceAnnotation
persistent identity: annotation2383930
display id: annotation2383930
version: 1
top level: BBa_K1399008
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1399008/annotation2383930/range2383930/1

https://synbiohub.org/public/igem/BBa_K1399008/annotation2383930/range2383930/1
type: Range
persistent identity: range2383930
display id: range2383930
version: 1
direction: direction/forward
top level: BBa_K1399008
start: 715
end: 747
orientation: inline

GFP (mut3b)
type: SequenceAnnotation
persistent identity: annotation2383929
display id: annotation2383929
version: 1
top level: BBa_K1399008
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1399008/annotation2383929/range2383929/1

https://synbiohub.org/public/igem/BBa_K1399008/annotation2383929/range2383929/1
type: Range
persistent identity: range2383929
display id: range2383929
version: 1
direction: direction/forward
top level: BBa_K1399008
start: 3
end: 714
orientation: inline

BBa_K1899002
type: ComponentDefinition
persistent identity: BBa K1899002
display id: BBa_K1899002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Blue Fluorescent Protein mTagBFP generator (Strong RBS)
creator: iGEM2016_HKUST
created: 2016-09-20T11:00:00Z
modified: 2016-09-21T09:47:40Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2365_
m user id: 26069
owner id: 26069
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Improved version of BBa_K592024 by adding a terminator BBa_B1006 3??? to this part.
mutable notes: NIL
mutable provenance: NIL
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_B1006
component: BBa_K592024
sequence annotation: BBa_B1006
sequence annotation: BBa_K592024
sequence: https://synbiohub.org/public/igem/BBa_K1899002_sequence/1

BBa_B1006
type: Component
persistent identity: component2484361
display id: component2484361
version: 1
top level: BBa_K1899002
access: public
definition: https://synbiohub.org/public/igem/BBa_B1006/1

BBa_K592024
type: Component
persistent identity: component2484356
display id: component2484356
version: 1
top level: BBa_K1899002
access: public
definition: https://synbiohub.org/public/igem/BBa_K592024/1

BBa_B1006
type: SequenceAnnotation
persistent identity: annotation2484361
display id: annotation2484361
version: 1
top level: BBa_K1899002
location: https://synbiohub.org/public/igem/BBa_K1899002/annotation2484361/range2484361/1
component: BBa_B1006

https://synbiohub.org/public/igem/BBa_K1899002/annotation2484361/range2484361/1
type: Range
persistent identity: range2484361
display id: range2484361
version: 1
direction: direction/reverse
top level: BBa_K1899002
start: 732
end: 770
orientation: inline

BBa_K592024
type: SequenceAnnotation
persistent identity: annotation2484356
display id: annotation2484356
version: 1
top level: BBa_K1899002
location: https://synbiohub.org/public/igem/BBa_K1899002/annotation2484356/range2484356/1
component: BBa_K592024

https://synbiohub.org/public/igem/BBa_K1899002/annotation2484356/range2484356/1
type: Range
persistent identity: range2484356
display id: range2484356
version: 1
direction: direction/reverse
top level: BBa_K1899002
start: 1
end: 723
orientation: inline

sfGFP(Sp)
type: ComponentDefinition
persistent identity: BBa K1930006
display id: BBa_K1930006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: sfGFP(Sp)
creator: Barbora Waclawikova
created: 2016-10-15T11:00:00Z
modified: 2016-10-16T05:16:06Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2397_
m user id: 29748
owner id: 29748
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This part is a reporter sfGFP(Sp) originally optimized for Streptococcus pneumoniae.
mutable notes: sfGFP originally optimized for streptococcus pneumonia because it could be shown that the signal in Bacillus is even brighter than the one from sfGFP(Bs).
mutable provenance: Plasmid pNW
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: sfGFP(Sp)
sequence: https://synbiohub.org/public/igem/BBa_K1930006_sequence/1

sfGFP(Sp)
type: SequenceAnnotation
persistent identity: annotation2513522
display id: annotation2513522
version: 1
top level: sfGFP(Sp)
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1930006/annotation2513522/range2513522/1

https://synbiohub.org/public/igem/BBa_K1930006/annotation2513522/range2513522/1
type: Range
persistent identity: range2513522
display id: range2513522
version: 1
direction: direction/forward
top level: sfGFP(Sp)
start: 1
end: 722
orientation: inline

BBa_K180009
type: ComponentDefinition
persistent identity: BBa K180009
display id: BBa_K180009
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: mBanana (rights owned by Clontech)
creator: Richard Wilson, Stephanie Chambers
created: 2009-10-15T11:00:00Z
modified: 2016-01-25T01:37:03Z
discontinued: false
dominant: false
experience: experience/None
group u list: _278_
m user id: 4206
owner id: 4082
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: mBanana fluorescent protein - Rights owned by Clontech. Sequence is commercially available from Clontech. Sequence obtained through PCR amplification, with BioBrick prefix and suffix added through in-house designed primers.
mutable notes: PstI site within the mBanana sequence - has to be assembled using BioBrick assembly in such an order that any successive digestions will not require PstI, otherwise the mCherry sequence will be cut as well as the site of interest. Alternatively, the assembly can be performed using non-standard BioBrick assembly. Part cannot be submitted to registry as the rights are owned by Clontech. The mCherry sequence is however commercially available from Clontech in the pmBanana vector.
mutable provenance: pmBanana vector, Clontech
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: mBanana
sequence: https://synbiohub.org/public/igem/BBa_K180009_sequence/1

mBanana
type: SequenceAnnotation
persistent identity: annotation2046843
display id: annotation2046843
version: 1
top level: BBa_K180009
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K180009/annotation2046843/range2046843/1

https://synbiohub.org/public/igem/BBa_K180009/annotation2046843/range2046843/1
type: Range
persistent identity: range2046843
display id: range2046843
version: 1
direction: direction/forward
top level: BBa_K180009
start: 1
end: 708
orientation: inline

ecfp
type: ComponentDefinition
persistent identity: BBa I15012
display id: BBa_I15012
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: B0030.ECFP
creator: Jeff Tabor
created: 2005-05-11T11:00:00Z
modified: 2015-08-31T04:07:38Z
discontinued: false
dominant: false
experience: experience/None
group u list: _5_
m user id: 0
owner id: 88
owning group id: 37
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part accepts POPS to produce ECFP under the strong RBS B0030
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_E0020
component: BBa_B0030
sequence annotation: BBa_E0020
sequence annotation: BBa_B0030
sequence: https://synbiohub.org/public/igem/BBa_I15012_sequence/1

BBa_E0020
type: Component
persistent identity: component1486919
display id: component1486919
version: 1
top level: ecfp
access: public
definition: ecfp

BBa_B0030
type: Component
persistent identity: component1486916
display id: component1486916
version: 1
top level: ecfp
access: public
definition: https://synbiohub.org/public/igem/BBa_B0030/1

BBa_E0020
type: SequenceAnnotation
persistent identity: annotation1486919
display id: annotation1486919
version: 1
top level: ecfp
location: https://synbiohub.org/public/igem/BBa_I15012/annotation1486919/range1486919/1
component: BBa_E0020

https://synbiohub.org/public/igem/BBa_I15012/annotation1486919/range1486919/1
type: Range
persistent identity: range1486919
display id: range1486919
version: 1
direction: direction/forward
top level: ecfp
start: 22
end: 744
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation1486916
display id: annotation1486916
version: 1
top level: ecfp
location: https://synbiohub.org/public/igem/BBa_I15012/annotation1486916/range1486916/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_I15012/annotation1486916/range1486916/1
type: Range
persistent identity: range1486916
display id: range1486916
version: 1
direction: direction/forward
top level: ecfp
start: 1
end: 15
orientation: inline

BBa_K1740001
type: ComponentDefinition
persistent identity: BBa K1740001
display id: BBa_K1740001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Dronpa145K ORF (E. coli optimized)
creator: Keisuke Sugahara, Momoko Saegusa
created: 2015-08-19T11:00:00Z
modified: 2016-01-25T11:16:53Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2162_
m user id: 4206
owner id: 25441
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This is test
mutable notes: none
mutable provenance: .
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Dronpa 145K
sequence annotation: 145Asn to 145Lys
sequence annotation: start codon
sequence: https://synbiohub.org/public/igem/BBa_K1740001_sequence/1

Dronpa 145K
type: SequenceAnnotation
persistent identity: annotation2469980
display id: annotation2469980
version: 1
top level: BBa_K1740001
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1740001/annotation2469980/range2469980/1

https://synbiohub.org/public/igem/BBa_K1740001/annotation2469980/range2469980/1
type: Range
persistent identity: range2469980
display id: range2469980
version: 1
direction: direction/forward
top level: BBa_K1740001
start: 1
end: 672
orientation: inline

145Asn to 145Lys
type: SequenceAnnotation
persistent identity: annotation2469984
display id: annotation2469984
version: 1
top level: BBa_K1740001
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K1740001/annotation2469984/range2469984/1

https://synbiohub.org/public/igem/BBa_K1740001/annotation2469984/range2469984/1
type: Range
persistent identity: range2469984
display id: range2469984
version: 1
direction: direction/forward
top level: BBa_K1740001
start: 433
end: 435
orientation: inline

start codon
type: SequenceAnnotation
persistent identity: annotation2469983
display id: annotation2469983
version: 1
top level: BBa_K1740001
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1740001/annotation2469983/range2469983/1

https://synbiohub.org/public/igem/BBa_K1740001/annotation2469983/range2469983/1
type: Range
persistent identity: range2469983
display id: range2469983
version: 1
direction: direction/forward
top level: BBa_K1740001
start: 1
end: 3
orientation: inline

SYFP2
type: ComponentDefinition
persistent identity: BBa K864100
display id: BBa_K864100
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Super Yellow Fluorescent Protein 2 (SYFP2)
creator: Erik Gullberg
created: 2012-09-23T11:00:00Z
modified: 2016-01-21T02:29:24Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1124_
m user id: 4206
owner id: 10137
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This part codes for the bright yellow fluorescent protein SYFP2. SYFP2 is a GFP based monomeric protein with a narrow fluorescence emission spectrum with a maximum at 515 nm. Bacteria expressing SYFP2 are reported to be 12 times brighter than those expressing EYFP(Q69K) and almost 2-fold brighter than bacteria expressing Venus (Ref. 10.1021/bi0516273). Codon optimized for expression in E coli by DNA 2.0. Mutations compared to wtGFP amino acid sequence (GenBank Accession number M62653) are F46L F64L S65G S72A M153T V163A S175G T203Y A206K.
mutable notes: The sequence has been codon optimized for expression in E coli by DNA 2.0.
mutable provenance: Amino acid sequence taken from the article by Kremers et al 2006.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: SYFP2
sequence: https://synbiohub.org/public/igem/BBa_K864100_sequence/1

SYFP2
type: SequenceAnnotation
persistent identity: annotation2196221
display id: annotation2196221
version: 1
top level: SYFP2
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K864100/annotation2196221/range2196221/1

https://synbiohub.org/public/igem/BBa_K864100/annotation2196221/range2196221/1
type: Range
persistent identity: range2196221
display id: range2196221
version: 1
direction: direction/forward
top level: SYFP2
start: 1
end: 717
orientation: inline

SP-Lipocal
type: ComponentDefinition
persistent identity: BBa K157036
display id: BBa_K157036
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: SP-Lipocalin-GGGSlinker-Tm-Fluolinker-cCFP
creator: iGEM Team Freiburg 2008
created: 2008-10-25T11:00:00Z
modified: 2015-05-08T01:10:55Z
discontinued: false
dominant: false
experience: experience/None
group u list: _232_
m user id: 0
owner id: 1673
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: A composite part consisting of the signal peptide of Erbb1/EGF-receptor, scFV-antiNIP, GGGSflexlinker (extracellular part), the trans-membrane region of the Erbb1/EGF-receptor and the C-terminal part of the split fluorophor YFP with a fluolinker joined to its N-Terminus (intracellular part).
mutable notes: none
mutable provenance: Derived by cloning of the parts BBa_K157001, BBa_K157004, BBa_K157013, BBa_K157002, BBa_K157009 and BBa_K157005. For further information see the description of the parts mentioned above.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: SP-Lipocalin-GGGSlinker-Tm-Fluolinker-cCFP
sequence annotation: NgoMI
sequence annotation: Scar (Thr, Gly)
sequence annotation: GGGS-Linker
sequence annotation: Tm
sequence annotation: Fluolinker
sequence annotation: SP
sequence annotation: AgeI
sequence annotation: Lipocalin
sequence annotation: C-Term_CFP
sequence: https://synbiohub.org/public/igem/BBa_K157036_sequence/1

SP-Lipocalin-GGGSlinker-Tm-Fluolinker-cCFP
type: SequenceAnnotation
persistent identity: annotation1993396
display id: annotation1993396
version: 1
top level: SP-Lipocal
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K157036/annotation1993396/range1993396/1

https://synbiohub.org/public/igem/BBa_K157036/annotation1993396/range1993396/1
type: Range
persistent identity: range1993396
display id: range1993396
version: 1
direction: direction/forward
top level: SP-Lipocal
start: 1
end: 1047
orientation: inline

NgoMI
type: SequenceAnnotation
persistent identity: annotation1993397
display id: annotation1993397
version: 1
top level: SP-Lipocal
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157036/annotation1993397/range1993397/1

https://synbiohub.org/public/igem/BBa_K157036/annotation1993397/range1993397/1
type: Range
persistent identity: range1993397
display id: range1993397
version: 1
direction: direction/forward
top level: SP-Lipocal
start: 4
end: 9
orientation: inline

Scar (Thr, Gly)
type: SequenceAnnotation
persistent identity: annotation1993401
display id: annotation1993401
version: 1
top level: SP-Lipocal
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157036/annotation1993401/range1993401/1

https://synbiohub.org/public/igem/BBa_K157036/annotation1993401/range1993401/1
type: Range
persistent identity: range1993401
display id: range1993401
version: 1
direction: direction/forward
top level: SP-Lipocal
start: 610
end: 615
orientation: inline

GGGS-Linker
type: SequenceAnnotation
persistent identity: annotation1993402
display id: annotation1993402
version: 1
top level: SP-Lipocal
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157036/annotation1993402/range1993402/1

https://synbiohub.org/public/igem/BBa_K157036/annotation1993402/range1993402/1
type: Range
persistent identity: range1993402
display id: range1993402
version: 1
direction: direction/forward
top level: SP-Lipocal
start: 616
end: 660
orientation: inline

Tm
type: SequenceAnnotation
persistent identity: annotation1993404
display id: annotation1993404
version: 1
top level: SP-Lipocal
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157036/annotation1993404/range1993404/1

https://synbiohub.org/public/igem/BBa_K157036/annotation1993404/range1993404/1
type: Range
persistent identity: range1993404
display id: range1993404
version: 1
direction: direction/forward
top level: SP-Lipocal
start: 667
end: 735
orientation: inline

Scar (Thr, Gly)
type: SequenceAnnotation
persistent identity: annotation1993407
display id: annotation1993407
version: 1
top level: SP-Lipocal
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157036/annotation1993407/range1993407/1

https://synbiohub.org/public/igem/BBa_K157036/annotation1993407/range1993407/1
type: Range
persistent identity: range1993407
display id: range1993407
version: 1
direction: direction/forward
top level: SP-Lipocal
start: 793
end: 798
orientation: inline

Fluolinker
type: SequenceAnnotation
persistent identity: annotation1993406
display id: annotation1993406
version: 1
top level: SP-Lipocal
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157036/annotation1993406/range1993406/1

https://synbiohub.org/public/igem/BBa_K157036/annotation1993406/range1993406/1
type: Range
persistent identity: range1993406
display id: range1993406
version: 1
direction: direction/forward
top level: SP-Lipocal
start: 742
end: 792
orientation: inline

SP
type: SequenceAnnotation
persistent identity: annotation1993398
display id: annotation1993398
version: 1
top level: SP-Lipocal
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157036/annotation1993398/range1993398/1

https://synbiohub.org/public/igem/BBa_K157036/annotation1993398/range1993398/1
type: Range
persistent identity: range1993398
display id: range1993398
version: 1
direction: direction/forward
top level: SP-Lipocal
start: 10
end: 81
orientation: inline

AgeI
type: SequenceAnnotation
persistent identity: annotation1993408
display id: annotation1993408
version: 1
top level: SP-Lipocal
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157036/annotation1993408/range1993408/1

https://synbiohub.org/public/igem/BBa_K157036/annotation1993408/range1993408/1
type: Range
persistent identity: range1993408
display id: range1993408
version: 1
direction: direction/forward
top level: SP-Lipocal
start: 1042
end: 1047
orientation: inline

Scar (Thr, Gly)
type: SequenceAnnotation
persistent identity: annotation1993399
display id: annotation1993399
version: 1
top level: SP-Lipocal
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157036/annotation1993399/range1993399/1

https://synbiohub.org/public/igem/BBa_K157036/annotation1993399/range1993399/1
type: Range
persistent identity: range1993399
display id: range1993399
version: 1
direction: direction/forward
top level: SP-Lipocal
start: 82
end: 87
orientation: inline

Lipocalin
type: SequenceAnnotation
persistent identity: annotation1993400
display id: annotation1993400
version: 1
top level: SP-Lipocal
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157036/annotation1993400/range1993400/1

https://synbiohub.org/public/igem/BBa_K157036/annotation1993400/range1993400/1
type: Range
persistent identity: range1993400
display id: range1993400
version: 1
direction: direction/forward
top level: SP-Lipocal
start: 88
end: 609
orientation: inline

Scar (Thr, Gly)
type: SequenceAnnotation
persistent identity: annotation1993405
display id: annotation1993405
version: 1
top level: SP-Lipocal
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157036/annotation1993405/range1993405/1

https://synbiohub.org/public/igem/BBa_K157036/annotation1993405/range1993405/1
type: Range
persistent identity: range1993405
display id: range1993405
version: 1
direction: direction/forward
top level: SP-Lipocal
start: 736
end: 741
orientation: inline

Scar (Thr, Gly)
type: SequenceAnnotation
persistent identity: annotation1993403
display id: annotation1993403
version: 1
top level: SP-Lipocal
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157036/annotation1993403/range1993403/1

https://synbiohub.org/public/igem/BBa_K157036/annotation1993403/range1993403/1
type: Range
persistent identity: range1993403
display id: range1993403
version: 1
direction: direction/forward
top level: SP-Lipocal
start: 661
end: 666
orientation: inline

C-Term_CFP
type: SequenceAnnotation
persistent identity: annotation1993395
display id: annotation1993395
version: 1
top level: SP-Lipocal
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157036/annotation1993395/range1993395/1

https://synbiohub.org/public/igem/BBa_K157036/annotation1993395/range1993395/1
type: Range
persistent identity: range1993395
display id: range1993395
version: 1
direction: direction/forward
top level: SP-Lipocal
start: 799
end: 1041
orientation: inline

BBa_K1399001
type: ComponentDefinition
persistent identity: BBa K1399001
display id: BBa_K1399001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RFP from Discosoma striata (coral) with LVA-ssrA degradation tag
creator: Anna Stikane
created: 2014-09-17T11:00:00Z
modified: 2015-05-08T01:10:15Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1777_
m user id: 0
owner id: 22477
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: Mutant RFP from Discosoma striata (coral) (see part BBa_E1010) with added LVA-ssrA degradation tag. The tag increases RFP turn-over rate, thus providing better temporal resolution of red fluorescence. In the same time, maximal fluorescence amplitudes will be lower as newly formed protein is degraded as soon as it is formed. This tag is commonly attached to repressor proteins for use in various gene networks (e.g., oscillators). The tag encodes peptide sequence AANDENYALVA and is recognized by ClpA and ClpX unfoldases and ClpX mediator SspB.[1] ClpA and ClpX then form a proteosome-like complex with ClpP protease and the protein is degraded.[1] The final three residues of the tag determines the strength of interaction with ClpX and thus the final protein degradation rate.[2] The LVA tag is reported to lead to fast protein degradation, degrading GFP with rate -0.018 per minute.[2] However, be aware that exact protein degradation rate depends on multiple factors: ClpXP and ClpAP protease and SspB mediator concentrations; protein stability; Km of binding to the protease; temperature [3]. References: [1] Flynn, J. M. et al. Overlapping recognition determinants within the ssrA degradation tag allow modulation of proteolysis. Proc. Natl. Acad. Sci. U. S. A. 98, 10584???9 (2001). [2] Andersen, J. B. et al. New unstable variants of green fluorescent protein for studies of transient gene expression in bacteria. Appl. Environ. Microbiol. 64, 2240???6 (1998). [3] Purcell, O., Grierson, C. S., Bernardo, M. Di & Savery, N. J. Temperature dependence of ssrA-tag mediated protein degradation. J. Biol. Eng. 6, 10 (2012).
mutable notes: The tag was attached to RFP using PCR and MABEL (mutagenesis with blunt-end ligation), thus avoiding introduction of additonal residues and restriction sites. Different parts of the tag are recognized by different proteins, for example, the final 3 residues (LVA in this case) are recognised by ClpX, whereas first 4 residues of the tag are required for efficient SspB binding.[1] Thus modifications of these critical residues alter the efficacy with what different proteases bind to it.
mutable provenance: RFP comes from part BBa_E1010, tag sequence was obtained from paper by Andersen et al., (1998).[2]
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: start
sequence annotation: stop
sequence annotation: cds
sequence annotation: LVA-ssrA tag
sequence: https://synbiohub.org/public/igem/BBa_K1399001_sequence/1

start
type: SequenceAnnotation
persistent identity: annotation2383872
display id: annotation2383872
version: 1
top level: BBa_K1399001
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1399001/annotation2383872/range2383872/1

https://synbiohub.org/public/igem/BBa_K1399001/annotation2383872/range2383872/1
type: Range
persistent identity: range2383872
display id: range2383872
version: 1
direction: direction/forward
top level: BBa_K1399001
start: 1
end: 3
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2383875
display id: annotation2383875
version: 1
top level: BBa_K1399001
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1399001/annotation2383875/range2383875/1

https://synbiohub.org/public/igem/BBa_K1399001/annotation2383875/range2383875/1
type: Range
persistent identity: range2383875
display id: range2383875
version: 1
direction: direction/forward
top level: BBa_K1399001
start: 709
end: 711
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2383876
display id: annotation2383876
version: 1
top level: BBa_K1399001
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1399001/annotation2383876/range2383876/1

https://synbiohub.org/public/igem/BBa_K1399001/annotation2383876/range2383876/1
type: Range
persistent identity: range2383876
display id: range2383876
version: 1
direction: direction/forward
top level: BBa_K1399001
start: 712
end: 714
orientation: inline

cds
type: SequenceAnnotation
persistent identity: annotation2383873
display id: annotation2383873
version: 1
top level: BBa_K1399001
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1399001/annotation2383873/range2383873/1

https://synbiohub.org/public/igem/BBa_K1399001/annotation2383873/range2383873/1
type: Range
persistent identity: range2383873
display id: range2383873
version: 1
direction: direction/forward
top level: BBa_K1399001
start: 4
end: 675
orientation: inline

LVA-ssrA tag
type: SequenceAnnotation
persistent identity: annotation2383874
display id: annotation2383874
version: 1
top level: BBa_K1399001
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1399001/annotation2383874/range2383874/1

https://synbiohub.org/public/igem/BBa_K1399001/annotation2383874/range2383874/1
type: Range
persistent identity: range2383874
display id: range2383874
version: 1
direction: direction/forward
top level: BBa_K1399001
start: 676
end: 708
orientation: inline

BBa_K1789003
type: ComponentDefinition
persistent identity: BBa K1789003
display id: BBa_K1789003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: GFP1(with termination codon)
creator: Xinyuan Qiu
created: 2015-09-13T11:00:00Z
modified: 2016-01-25T01:04:29Z
discontinued: false
dominant: false
group u list: _2214_
m user id: 4206
owner id: 21134
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This part is the Amino Half of GFP with termination codon
mutable notes: Because of the need of our project, we added a termination codon when designing the PCR.
mutable provenance: This part is gained from part BBa_I715019. we added the termination codon when designing the PCR.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: GFP1
sequence annotation: termination codon
sequence: https://synbiohub.org/public/igem/BBa_K1789003_sequence/1

GFP1
type: SequenceAnnotation
persistent identity: annotation2454065
display id: annotation2454065
version: 1
top level: BBa_K1789003
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1789003/annotation2454065/range2454065/1

https://synbiohub.org/public/igem/BBa_K1789003/annotation2454065/range2454065/1
type: Range
persistent identity: range2454065
display id: range2454065
version: 1
direction: direction/forward
top level: BBa_K1789003
start: 1
end: 471
orientation: inline

termination codon
type: SequenceAnnotation
persistent identity: annotation2454066
display id: annotation2454066
version: 1
top level: BBa_K1789003
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1789003/annotation2454066/range2454066/1

https://synbiohub.org/public/igem/BBa_K1789003/annotation2454066/range2454066/1
type: Range
persistent identity: range2454066
display id: range2454066
version: 1
direction: direction/forward
top level: BBa_K1789003
start: 472
end: 474
orientation: inline

mCherry
type: ComponentDefinition
persistent identity: BBa E2060
display id: BBa_E2060
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: derivative of mRFP1, yeast-optimized
creator: ryu
created: 2005-01-24T12:00:00Z
modified: 2015-08-31T04:07:27Z
discontinued: false
dominant: false
experience: experience/None
group u list: _8_
m user id: 0
owner id: 230
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: mRFP (DsRed) derived, Ex587/Em610, yeast codon optimized
mutable notes: Codon optimized for yeast. Contains N- and C-terminal linker sequences (with homology to biobrick parts BBa_E2050 (mOrange), BBa_E2020 (Cerulean CFP), and BBa_E2030 (Venus YFP) to facilitate color swapping in yeast. Adds N-"MATSG" and "GSGTA"-C. to published amino acid sequence. Double TAATAA stop codon. Missing EcoRI, HindIII, NotI, NdeI, XhoI, RsrII, BamHI, NcoI, BglI, SpeI, XbaI, and PstI. except for 5' and 3' ends no significant sequence identity runs to mOrange (BBa_E2050).
mutable provenance: Shaner et al, 2004. Nat Biotech (22):1567-1571
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: mCherry
sequence annotation: GSGTA linker
sequence annotation: Help:Barcodes
sequence annotation: MATSG linker
sequence: https://synbiohub.org/public/igem/BBa_E2060_sequence/1

mCherry
type: SequenceAnnotation
persistent identity: annotation1431932
display id: annotation1431932
version: 1
top level: mCherry
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_E2060/annotation1431932/range1431932/1

https://synbiohub.org/public/igem/BBa_E2060/annotation1431932/range1431932/1
type: Range
persistent identity: range1431932
display id: range1431932
version: 1
direction: direction/forward
top level: mCherry
start: 1
end: 738
orientation: inline

GSGTA linker
type: SequenceAnnotation
persistent identity: annotation1431931
display id: annotation1431931
version: 1
top level: mCherry
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_E2060/annotation1431931/range1431931/1

https://synbiohub.org/public/igem/BBa_E2060/annotation1431931/range1431931/1
type: Range
persistent identity: range1431931
display id: range1431931
version: 1
direction: direction/forward
top level: mCherry
start: 724
end: 738
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2214037
display id: annotation2214037
version: 1
top level: mCherry
role: SO:0000807
role: feature/barcode
location: https://synbiohub.org/public/igem/BBa_E2060/annotation2214037/range2214037/1

https://synbiohub.org/public/igem/BBa_E2060/annotation2214037/range2214037/1
type: Range
persistent identity: range2214037
display id: range2214037
version: 1
direction: direction/forward
top level: mCherry
start: 745
end: 769
orientation: inline

MATSG linker
type: SequenceAnnotation
persistent identity: annotation1431927
display id: annotation1431927
version: 1
top level: mCherry
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_E2060/annotation1431927/range1431927/1

https://synbiohub.org/public/igem/BBa_E2060/annotation1431927/range1431927/1
type: Range
persistent identity: range1431927
display id: range1431927
version: 1
direction: direction/forward
top level: mCherry
start: 1
end: 15
orientation: inline

BBa_K1031302
type: ComponentDefinition
persistent identity: BBa K1031302
display id: BBa_K1031302
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Pc-B0032-sfGFP-Terminator (HbpR)
creator: He Shuaixin
created: 2013-09-08T11:00:00Z
modified: 2015-05-08T01:08:47Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1338_
m user id: 0
owner id: 15843
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Ph-32-sfGFP-TT (HbpR)
mutable notes: molecular cloning
mutable provenance: synthesis, lab made
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: Ph
sequence annotation: sfGFP
sequence annotation: BBa_B0015
sequence annotation: BBa_B0032
sequence: https://synbiohub.org/public/igem/BBa_K1031302_sequence/1

Ph
type: SequenceAnnotation
persistent identity: annotation2360436
display id: annotation2360436
version: 1
top level: BBa_K1031302
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1031302/annotation2360436/range2360436/1

https://synbiohub.org/public/igem/BBa_K1031302/annotation2360436/range2360436/1
type: Range
persistent identity: range2360436
display id: range2360436
version: 1
direction: direction/forward
top level: BBa_K1031302
start: 1
end: 229
orientation: inline

sfGFP
type: SequenceAnnotation
persistent identity: annotation2360438
display id: annotation2360438
version: 1
top level: BBa_K1031302
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1031302/annotation2360438/range2360438/1

https://synbiohub.org/public/igem/BBa_K1031302/annotation2360438/range2360438/1
type: Range
persistent identity: range2360438
display id: range2360438
version: 1
direction: direction/forward
top level: BBa_K1031302
start: 254
end: 976
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2360439
display id: annotation2360439
version: 1
top level: BBa_K1031302
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1031302/annotation2360439/range2360439/1

https://synbiohub.org/public/igem/BBa_K1031302/annotation2360439/range2360439/1
type: Range
persistent identity: range2360439
display id: range2360439
version: 1
direction: direction/forward
top level: BBa_K1031302
start: 983
end: 1111
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation2360437
display id: annotation2360437
version: 1
top level: BBa_K1031302
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1031302/annotation2360437/range2360437/1

https://synbiohub.org/public/igem/BBa_K1031302/annotation2360437/range2360437/1
type: Range
persistent identity: range2360437
display id: range2360437
version: 1
direction: direction/forward
top level: BBa_K1031302
start: 236
end: 247
orientation: inline

BBa_K1486023
type: ComponentDefinition
persistent identity: BBa K1486023
display id: BBa_K1486023
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Yeast optimized superfolder GFP
creator: EPFL iGem team 2014
created: 2014-09-25T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: To edit
mutable notes: To edit
mutable provenance: To edit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: Yo sfGFP
sequence: https://synbiohub.org/public/igem/BBa_K1486023_sequence/1

Yo sfGFP
type: SequenceAnnotation
persistent identity: annotation2418775
display id: annotation2418775
version: 1
top level: BBa_K1486023
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1486023/annotation2418775/range2418775/1

https://synbiohub.org/public/igem/BBa_K1486023/annotation2418775/range2418775/1
type: Range
persistent identity: range2418775
display id: range2418775
version: 1
direction: direction/forward
top level: BBa_K1486023
start: 1
end: 717
orientation: inline

BBa_K777133
type: ComponentDefinition
persistent identity: BBa K777133
display id: BBa_K777133
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Similar to pSB1C3, with RFP reporter
creator: Team Goettingen
created: 2012-09-27T11:00:00Z
modified: 2015-05-08T01:13:17Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1029_
m user id: 0
owner id: 12106
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This part is similar to [http://partsregistry.org/Part:BBa_J61002 J61002] but the backbone is based on pSB1C3 and carries a chloramphenicol resistance.
mutable notes: * No specific design considerations.
mutable provenance: * This part is a compination of E1010, B0034 and pSB1C3 from the registry.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: Chloramphenicol resistance
sequence annotation: mRFP1
sequence annotation: VF2 primer binding site
sequence annotation: rep (pMB1)
sequence annotation: XbaI
sequence annotation: ORI
sequence annotation: EcoRI
sequence annotation: SpeI
sequence annotation: VR primer binding site
sequence annotation: RBS
sequence annotation: BB0034
sequence annotation: E1010
sequence annotation: PstI
sequence: https://synbiohub.org/public/igem/BBa_K777133_sequence/1

Chloramphenicol resistance
type: SequenceAnnotation
persistent identity: annotation2203657
display id: annotation2203657
version: 1
top level: BBa_K777133
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K777133/annotation2203657/range2203657/1

https://synbiohub.org/public/igem/BBa_K777133/annotation2203657/range2203657/1
type: Range
persistent identity: range2203657
display id: range2203657
version: 1
direction: direction/reverse
top level: BBa_K777133
start: 2028
end: 2687
orientation: inline

mRFP1
type: SequenceAnnotation
persistent identity: annotation2203653
display id: annotation2203653
version: 1
top level: BBa_K777133
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K777133/annotation2203653/range2203653/1

https://synbiohub.org/public/igem/BBa_K777133/annotation2203653/range2203653/1
type: Range
persistent identity: range2203653
display id: range2203653
version: 1
direction: direction/forward
top level: BBa_K777133
start: 26
end: 703
orientation: inline

VF2 primer binding site
type: SequenceAnnotation
persistent identity: annotation2203664
display id: annotation2203664
version: 1
top level: BBa_K777133
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K777133/annotation2203664/range2203664/1

https://synbiohub.org/public/igem/BBa_K777133/annotation2203664/range2203664/1
type: Range
persistent identity: range2203664
display id: range2203664
version: 1
direction: direction/forward
top level: BBa_K777133
start: 2799
end: 2818
orientation: inline

rep (pMB1)
type: SequenceAnnotation
persistent identity: annotation2203655
display id: annotation2203655
version: 1
top level: BBa_K777133
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K777133/annotation2203655/range2203655/1

https://synbiohub.org/public/igem/BBa_K777133/annotation2203655/range2203655/1
type: Range
persistent identity: range2203655
display id: range2203655
version: 1
direction: direction/forward
top level: BBa_K777133
start: 1129
end: 1735
orientation: inline

XbaI
type: SequenceAnnotation
persistent identity: annotation2203668
display id: annotation2203668
version: 1
top level: BBa_K777133
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K777133/annotation2203668/range2203668/1

https://synbiohub.org/public/igem/BBa_K777133/annotation2203668/range2203668/1
type: Range
persistent identity: range2203668
display id: range2203668
version: 1
direction: direction/forward
top level: BBa_K777133
start: 2932
end: 2937
orientation: inline

ORI
type: SequenceAnnotation
persistent identity: annotation2203662
display id: annotation2203662
version: 1
top level: BBa_K777133
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K777133/annotation2203662/range2203662/1

https://synbiohub.org/public/igem/BBa_K777133/annotation2203662/range2203662/1
type: Range
persistent identity: range2203662
display id: range2203662
version: 1
direction: direction/forward
top level: BBa_K777133
start: 1144
end: 1144
orientation: inline

EcoRI
type: SequenceAnnotation
persistent identity: annotation2203667
display id: annotation2203667
version: 1
top level: BBa_K777133
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K777133/annotation2203667/range2203667/1

https://synbiohub.org/public/igem/BBa_K777133/annotation2203667/range2203667/1
type: Range
persistent identity: range2203667
display id: range2203667
version: 1
direction: direction/forward
top level: BBa_K777133
start: 2917
end: 2922
orientation: inline

SpeI
type: SequenceAnnotation
persistent identity: annotation2203665
display id: annotation2203665
version: 1
top level: BBa_K777133
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K777133/annotation2203665/range2203665/1

https://synbiohub.org/public/igem/BBa_K777133/annotation2203665/range2203665/1
type: Range
persistent identity: range2203665
display id: range2203665
version: 1
direction: direction/forward
top level: BBa_K777133
start: 1
end: 6
orientation: inline

VR primer binding site
type: SequenceAnnotation
persistent identity: annotation2203663
display id: annotation2203663
version: 1
top level: BBa_K777133
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K777133/annotation2203663/range2203663/1

https://synbiohub.org/public/igem/BBa_K777133/annotation2203663/range2203663/1
type: Range
persistent identity: range2203663
display id: range2203663
version: 1
direction: direction/forward
top level: BBa_K777133
start: 1023
end: 1042
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2203651
display id: annotation2203651
version: 1
top level: BBa_K777133
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K777133/annotation2203651/range2203651/1

https://synbiohub.org/public/igem/BBa_K777133/annotation2203651/range2203651/1
type: Range
persistent identity: range2203651
display id: range2203651
version: 1
direction: direction/forward
top level: BBa_K777133
start: 8
end: 19
orientation: inline

BB0034
type: SequenceAnnotation
persistent identity: annotation2203652
display id: annotation2203652
version: 1
top level: BBa_K777133
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K777133/annotation2203652/range2203652/1

https://synbiohub.org/public/igem/BBa_K777133/annotation2203652/range2203652/1
type: Range
persistent identity: range2203652
display id: range2203652
version: 1
direction: direction/forward
top level: BBa_K777133
start: 8
end: 19
orientation: inline

E1010
type: SequenceAnnotation
persistent identity: annotation2203654
display id: annotation2203654
version: 1
top level: BBa_K777133
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K777133/annotation2203654/range2203654/1

https://synbiohub.org/public/igem/BBa_K777133/annotation2203654/range2203654/1
type: Range
persistent identity: range2203654
display id: range2203654
version: 1
direction: direction/forward
top level: BBa_K777133
start: 26
end: 703
orientation: inline

PstI
type: SequenceAnnotation
persistent identity: annotation2203666
display id: annotation2203666
version: 1
top level: BBa_K777133
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K777133/annotation2203666/range2203666/1

https://synbiohub.org/public/igem/BBa_K777133/annotation2203666/range2203666/1
type: Range
persistent identity: range2203666
display id: range2203666
version: 1
direction: direction/forward
top level: BBa_K777133
start: 884
end: 889
orientation: inline

Tm-nYFP
type: ComponentDefinition
persistent identity: BBa K157015
display id: BBa_K157015
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Tm-nYFP
creator: iGEM Team Freiburg 2008
created: 2008-10-25T11:00:00Z
modified: 2015-05-08T01:10:54Z
discontinued: false
dominant: false
experience: experience/None
group u list: _232_
m user id: 0
owner id: 1675
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: N-terminal part of the Split-flourophore YFP, fused to the trans-membrane region of the Erbb1/EGF-receptor.
mutable notes: none
mutable provenance: Derived by cloning the parts BBa_K157002, BBa_K157008. For further information see the description of the parts mentioned above.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: Scar (Thr, Gly)
sequence annotation: NgoMI
sequence annotation: N-Term_YFP
sequence annotation: Tm-nYFP
sequence annotation: Tm
sequence annotation: AgeI
sequence: https://synbiohub.org/public/igem/BBa_K157015_sequence/1

Scar (Thr, Gly)
type: SequenceAnnotation
persistent identity: annotation1991278
display id: annotation1991278
version: 1
top level: Tm-nYFP
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157015/annotation1991278/range1991278/1

https://synbiohub.org/public/igem/BBa_K157015/annotation1991278/range1991278/1
type: Range
persistent identity: range1991278
display id: range1991278
version: 1
direction: direction/forward
top level: Tm-nYFP
start: 79
end: 84
orientation: inline

NgoMI
type: SequenceAnnotation
persistent identity: annotation1989841
display id: annotation1989841
version: 1
top level: Tm-nYFP
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157015/annotation1989841/range1989841/1

https://synbiohub.org/public/igem/BBa_K157015/annotation1989841/range1989841/1
type: Range
persistent identity: range1989841
display id: range1989841
version: 1
direction: direction/forward
top level: Tm-nYFP
start: 4
end: 9
orientation: inline

N-Term_YFP
type: SequenceAnnotation
persistent identity: annotation1991279
display id: annotation1991279
version: 1
top level: Tm-nYFP
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157015/annotation1991279/range1991279/1

https://synbiohub.org/public/igem/BBa_K157015/annotation1991279/range1991279/1
type: Range
persistent identity: range1991279
display id: range1991279
version: 1
direction: direction/forward
top level: Tm-nYFP
start: 84
end: 552
orientation: inline

Tm-nYFP
type: SequenceAnnotation
persistent identity: annotation1989844
display id: annotation1989844
version: 1
top level: Tm-nYFP
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K157015/annotation1989844/range1989844/1

https://synbiohub.org/public/igem/BBa_K157015/annotation1989844/range1989844/1
type: Range
persistent identity: range1989844
display id: range1989844
version: 1
direction: direction/forward
top level: Tm-nYFP
start: 1
end: 558
orientation: inline

Tm
type: SequenceAnnotation
persistent identity: annotation1991277
display id: annotation1991277
version: 1
top level: Tm-nYFP
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157015/annotation1991277/range1991277/1

https://synbiohub.org/public/igem/BBa_K157015/annotation1991277/range1991277/1
type: Range
persistent identity: range1991277
display id: range1991277
version: 1
direction: direction/forward
top level: Tm-nYFP
start: 10
end: 78
orientation: inline

AgeI
type: SequenceAnnotation
persistent identity: annotation1989842
display id: annotation1989842
version: 1
top level: Tm-nYFP
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157015/annotation1989842/range1989842/1

https://synbiohub.org/public/igem/BBa_K157015/annotation1989842/range1989842/1
type: Range
persistent identity: range1989842
display id: range1989842
version: 1
direction: direction/forward
top level: Tm-nYFP
start: 553
end: 558
orientation: inline

BBa_K1680019
type: ComponentDefinition
persistent identity: BBa K1680019
display id: BBa_K1680019
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Cre-dronpa fusion
creator: Nicolai von Kuegelgen
created: 2015-09-16T11:00:00Z
modified: 2016-01-26T10:15:55Z
discontinued: false
dominant: false
group u list: _2098_
m user id: 4206
owner id: 26366
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Protein coding region for a fusion construct of Cre Recombinase and 145N Dronpa with 12 amino acid linker. Oligomerisation of Dronpa should stop Cre from accessing DNA in the nucleus thereby inhibiting it until light deactivation of dronpa.
mutable notes: 3A assembly using RFC25
mutable provenance: gBlocks designed by Team Tuebingen
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: K1680002
sequence annotation: K1680007
sequence annotation: K1680006
sequence: https://synbiohub.org/public/igem/BBa_K1680019_sequence/1

K1680002
type: SequenceAnnotation
persistent identity: annotation2477415
display id: annotation2477415
version: 1
top level: BBa_K1680019
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1680019/annotation2477415/range2477415/1

https://synbiohub.org/public/igem/BBa_K1680019/annotation2477415/range2477415/1
type: Range
persistent identity: range2477415
display id: range2477415
version: 1
direction: direction/forward
top level: BBa_K1680019
start: 1
end: 30
orientation: inline

K1680007
type: SequenceAnnotation
persistent identity: annotation2477416
display id: annotation2477416
version: 1
top level: BBa_K1680019
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1680019/annotation2477416/range2477416/1

https://synbiohub.org/public/igem/BBa_K1680019/annotation2477416/range2477416/1
type: Range
persistent identity: range2477416
display id: range2477416
version: 1
direction: direction/forward
top level: BBa_K1680019
start: 31
end: 1059
orientation: inline

K1680002
type: SequenceAnnotation
persistent identity: annotation2477417
display id: annotation2477417
version: 1
top level: BBa_K1680019
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1680019/annotation2477417/range2477417/1

https://synbiohub.org/public/igem/BBa_K1680019/annotation2477417/range2477417/1
type: Range
persistent identity: range2477417
display id: range2477417
version: 1
direction: direction/forward
top level: BBa_K1680019
start: 1060
end: 1095
orientation: inline

K1680006
type: SequenceAnnotation
persistent identity: annotation2477418
display id: annotation2477418
version: 1
top level: BBa_K1680019
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1680019/annotation2477418/range2477418/1

https://synbiohub.org/public/igem/BBa_K1680019/annotation2477418/range2477418/1
type: Range
persistent identity: range2477418
display id: range2477418
version: 1
direction: direction/forward
top level: BBa_K1680019
start: 1096
end: 1764
orientation: inline

BBa_K1197010
type: ComponentDefinition
persistent identity: BBa K1197010
display id: BBa_K1197010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Pveg (K143012) + RBS (SpoVG) + RFP + DT
creator: Emre İlpars
created: 2013-09-21T11:00:00Z
modified: 2015-06-08T11:41:51Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1511_
m user id: 4206
owner id: 12422
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: .
mutable notes: .
mutable provenance: .
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K143012
component: BBa_K606013
sequence annotation: BBa_K606013
sequence annotation: BBa_K143012
sequence: https://synbiohub.org/public/igem/BBa_K1197010_sequence/1

BBa_K143012
type: Component
persistent identity: component2358047
display id: component2358047
version: 1
top level: BBa_K1197010
access: public
definition: https://synbiohub.org/public/igem/BBa_K143012/1

BBa_K606013
type: Component
persistent identity: component2358061
display id: component2358061
version: 1
top level: BBa_K1197010
access: public
definition: https://synbiohub.org/public/igem/BBa_K606013/1

BBa_K606013
type: SequenceAnnotation
persistent identity: annotation2358061
display id: annotation2358061
version: 1
top level: BBa_K1197010
location: https://synbiohub.org/public/igem/BBa_K1197010/annotation2358061/range2358061/1
component: BBa_K606013

https://synbiohub.org/public/igem/BBa_K1197010/annotation2358061/range2358061/1
type: Range
persistent identity: range2358061
display id: range2358061
version: 1
direction: direction/reverse
top level: BBa_K1197010
start: 106
end: 966
orientation: inline

BBa_K143012
type: SequenceAnnotation
persistent identity: annotation2358047
display id: annotation2358047
version: 1
top level: BBa_K1197010
location: https://synbiohub.org/public/igem/BBa_K1197010/annotation2358047/range2358047/1
component: BBa_K143012

https://synbiohub.org/public/igem/BBa_K1197010/annotation2358047/range2358047/1
type: Range
persistent identity: range2358047
display id: range2358047
version: 1
direction: direction/reverse
top level: BBa_K1197010
start: 1
end: 97
orientation: inline

BBa_K1031223
type: ComponentDefinition
persistent identity: BBa K1031223
display id: BBa_K1031223
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Po-B0033-sfGFP-Terminator (DmpR)
creator: He Shuaixin
created: 2013-09-08T11:00:00Z
modified: 2015-05-08T01:08:47Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1338_
m user id: 0
owner id: 15843
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Po-33-sfGFP-TT (DmpR)
mutable notes: molecular cloning
mutable provenance: synthesis, lab made
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: b0033
sequence annotation: B0015
sequence annotation: sfGFP
sequence annotation: po promoter
sequence: https://synbiohub.org/public/igem/BBa_K1031223_sequence/1

b0033
type: SequenceAnnotation
persistent identity: annotation2354359
display id: annotation2354359
version: 1
top level: BBa_K1031223
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1031223/annotation2354359/range2354359/1

https://synbiohub.org/public/igem/BBa_K1031223/annotation2354359/range2354359/1
type: Range
persistent identity: range2354359
display id: range2354359
version: 1
direction: direction/forward
top level: BBa_K1031223
start: 180
end: 190
orientation: inline

B0015
type: SequenceAnnotation
persistent identity: annotation2354361
display id: annotation2354361
version: 1
top level: BBa_K1031223
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1031223/annotation2354361/range2354361/1

https://synbiohub.org/public/igem/BBa_K1031223/annotation2354361/range2354361/1
type: Range
persistent identity: range2354361
display id: range2354361
version: 1
direction: direction/forward
top level: BBa_K1031223
start: 928
end: 1056
orientation: inline

sfGFP
type: SequenceAnnotation
persistent identity: annotation2354360
display id: annotation2354360
version: 1
top level: BBa_K1031223
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1031223/annotation2354360/range2354360/1

https://synbiohub.org/public/igem/BBa_K1031223/annotation2354360/range2354360/1
type: Range
persistent identity: range2354360
display id: range2354360
version: 1
direction: direction/forward
top level: BBa_K1031223
start: 197
end: 919
orientation: inline

po promoter
type: SequenceAnnotation
persistent identity: annotation2354358
display id: annotation2354358
version: 1
top level: BBa_K1031223
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1031223/annotation2354358/range2354358/1

https://synbiohub.org/public/igem/BBa_K1031223/annotation2354358/range2354358/1
type: Range
persistent identity: range2354358
display id: range2354358
version: 1
direction: direction/forward
top level: BBa_K1031223
start: 1
end: 171
orientation: inline

no ATG GFP
type: ComponentDefinition
persistent identity: BBa K579000
display id: BBa_K579000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: no ATG GFP
creator: Reo Yoshimura,Toshihiro Matsunami,Takahiro Nakagawa,Jyunpei Yokoigawa
created: 2011-10-04T11:00:00Z
modified: 2015-05-08T01:12:46Z
discontinued: false
dominant: true
experience: experience/None
group u list: _750_
m user id: 0
owner id: 7778
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: This plasmid vector was designed to fuse GFP to C-terminal regions of any proteins.(We use BBa_E0240.) In our projects, we use this plasmid to fuse GFP to dMLF, DIAP2, API2-MALT1 and others to monitor expression of these proteins in E. coli and Drosophila.
mutable notes: Primer sequence F:TTGAATTCTTTTCTAGATTTCGTAAAGGAGAAGAA R:AAACTGCAGAAAACTAGTTTTTTATTATTTGTATAG
mutable provenance: 820base sequence AAACCCATAATATCATGACATACCCTATAAAAATAGGCGTATCACGAGGCAGAATTCAGATAAAAAAAATCCCTAGCTTTCGCTAAGGATGATTCTGGAATTCTTTCTAGATTCGTAAAGGAGAAGAACTTTTCACTGGAGTTGTCCCAATTCTTGTTGAATTAGATGGTGATGTTAATGGGCACAAATTTTCTGTCAGTGGAGAGGGTGAAGGTGATGCAACATACGGAAAACTTACCCTTAAATTTATTTGCACTACTGGAAAACTACCTGTTCCATGGCCAACACTTGTCACTACTTTCGGTTATGGTGTTCAATGCTTTGCGAGATACCCAGATCATATGAAACAGCATGACTTTTTCAAGAGTGCCATGCCCGAAGGTTATGTACAGGAAAGAACTATATTTTTCAAAGATGACGGGAACTACAAGACACGTGCTGAAGTCAAGTTTGAAGGTGATACCCTTGTTAATAGAATCGAGTTAAAAGGTATTGATTTTAAAGAAGATGGAAACATTCTTGGACACAAATTGGAATACAACTATAACTCACACAATGTATACATCATGGCAGACAAACAAAAGAATGGAATCAAAGTTAACTTCAAAATTAGACACAACATTGAAGATGGAAGCGTTCAACTAGCAGACCATTATCAACAAAATACTCCAATTGGCGATGGCCCTGTCCTTTTACCAGACAACCATTACCTGTCCACACAATCTGCCCTTTCGAAAGATCCCAACGAAAAGAGAGACCACATGGTCCTTCTTGAGTTTGTAACAGCTGCTGGGATTACACATGGCATGGATTTTAATCT
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: BBa_K579000
sequence annotation: no ATG GFP
sequence: https://synbiohub.org/public/igem/BBa_K579000_sequence/1

BBa_K579000
type: SequenceAnnotation
persistent identity: annotation2155666
display id: annotation2155666
version: 1
top level: no ATG GFP
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K579000/annotation2155666/range2155666/1

https://synbiohub.org/public/igem/BBa_K579000/annotation2155666/range2155666/1
type: Range
persistent identity: range2155666
display id: range2155666
version: 1
direction: direction/forward
top level: no ATG GFP
start: 1
end: 717
orientation: inline

no ATG GFP
type: SequenceAnnotation
persistent identity: annotation2155667
display id: annotation2155667
version: 1
top level: no ATG GFP
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K579000/annotation2155667/range2155667/1

https://synbiohub.org/public/igem/BBa_K579000/annotation2155667/range2155667/1
type: Range
persistent identity: range2155667
display id: range2155667
version: 1
direction: direction/forward
top level: no ATG GFP
start: 1
end: 717
orientation: inline

BBa_E2030
type: ComponentDefinition
persistent identity: BBa E2030
display id: BBa_E2030
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: enhanced version of EYFP, yeast-optimized
creator: ryu
created: 2005-01-24T12:00:00Z
modified: 2015-08-31T04:07:26Z
discontinued: false
dominant: false
experience: experience/None
group u list: _8_
m user id: 0
owner id: 230
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Venus YFP, yeast codon optimized. EX515/EM528
mutable notes: Contains N- and C-terminal linker sequences (with homology to biobrick parts BBa_E2060 (mCherry), BBa_E2050 (mOrange), and BBa_E2020 (Cerulean CFP) to facilitate color swapping in yeast. Adds N-"MATSG" and "GSGTA"-C. to published amino acid sequence. Inserts a "V" after normal start M. Double TAATAA stop codon. Missing EcoRI, HindIII, NotI, NdeI, XhoI, RsrII, BamHI, NcoI, BglI, SpeI, XbaI, and PstI. Except for 5' and 3' ends no significant sequence identity runs with Cerulean CFP (BBa_E2020) or to GFP S65T as found in O'Shea deletion strain collection (originally from plasmid pFA6-GFP(S65T)-His3MX6).
mutable provenance: nagai T. et al. (2002) Nat Biot (20):87-90
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: Venus YFP
sequence annotation: GSGTA linker
sequence annotation: Help:Barcodes
sequence annotation: MATSG linker
sequence: https://synbiohub.org/public/igem/BBa_E2030_sequence/1

Venus YFP
type: SequenceAnnotation
persistent identity: annotation1431978
display id: annotation1431978
version: 1
top level: BBa_E2030
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_E2030/annotation1431978/range1431978/1

https://synbiohub.org/public/igem/BBa_E2030/annotation1431978/range1431978/1
type: Range
persistent identity: range1431978
display id: range1431978
version: 1
direction: direction/forward
top level: BBa_E2030
start: 1
end: 747
orientation: inline

GSGTA linker
type: SequenceAnnotation
persistent identity: annotation1431980
display id: annotation1431980
version: 1
top level: BBa_E2030
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_E2030/annotation1431980/range1431980/1

https://synbiohub.org/public/igem/BBa_E2030/annotation1431980/range1431980/1
type: Range
persistent identity: range1431980
display id: range1431980
version: 1
direction: direction/forward
top level: BBa_E2030
start: 733
end: 747
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2214039
display id: annotation2214039
version: 1
top level: BBa_E2030
role: SO:0000807
role: feature/barcode
location: https://synbiohub.org/public/igem/BBa_E2030/annotation2214039/range2214039/1

https://synbiohub.org/public/igem/BBa_E2030/annotation2214039/range2214039/1
type: Range
persistent identity: range2214039
display id: range2214039
version: 1
direction: direction/forward
top level: BBa_E2030
start: 754
end: 778
orientation: inline

MATSG linker
type: SequenceAnnotation
persistent identity: annotation1431979
display id: annotation1431979
version: 1
top level: BBa_E2030
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_E2030/annotation1431979/range1431979/1

https://synbiohub.org/public/igem/BBa_E2030/annotation1431979/range1431979/1
type: Range
persistent identity: range1431979
display id: range1431979
version: 1
direction: direction/forward
top level: BBa_E2030
start: 1
end: 15
orientation: inline

BBa_K1020012
type: ComponentDefinition
persistent identity: BBa K1020012
display id: BBa_K1020012
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Alkane Selector for intracellular alkane production
creator: Yue Liu
created: 2013-09-09T11:00:00Z
modified: 2015-05-08T01:08:43Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1327_
m user id: 0
owner id: 16881
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1020010
component: BBa_K1020003
sequence annotation: BBa_K1020003
sequence annotation: BBa_K1020010
sequence: https://synbiohub.org/public/igem/BBa_K1020012_sequence/1

BBa_K1020010
type: Component
persistent identity: component2373789
display id: component2373789
version: 1
top level: BBa_K1020012
access: public
definition: https://synbiohub.org/public/igem/BBa_K1020010/1

BBa_K1020003
type: Component
persistent identity: component2373802
display id: component2373802
version: 1
top level: BBa_K1020012
access: public
definition: https://synbiohub.org/public/igem/BBa_K1020003/1

BBa_K1020003
type: SequenceAnnotation
persistent identity: annotation2373802
display id: annotation2373802
version: 1
top level: BBa_K1020012
location: https://synbiohub.org/public/igem/BBa_K1020012/annotation2373802/range2373802/1
component: BBa_K1020003

https://synbiohub.org/public/igem/BBa_K1020012/annotation2373802/range2373802/1
type: Range
persistent identity: range2373802
display id: range2373802
version: 1
direction: direction/reverse
top level: BBa_K1020012
start: 1538
end: 2662
orientation: inline

BBa_K1020010
type: SequenceAnnotation
persistent identity: annotation2373789
display id: annotation2373789
version: 1
top level: BBa_K1020012
location: https://synbiohub.org/public/igem/BBa_K1020012/annotation2373789/range2373789/1
component: BBa_K1020010

https://synbiohub.org/public/igem/BBa_K1020012/annotation2373789/range2373789/1
type: Range
persistent identity: range2373789
display id: range2373789
version: 1
direction: direction/reverse
top level: BBa_K1020012
start: 1
end: 1529
orientation: inline

BBa_K389052
type: ComponentDefinition
persistent identity: BBa K389052
display id: BBa_K389052
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Tightly regulated lacO + mRFP
creator: Jonas Aretz
created: 2010-10-07T11:00:00Z
modified: 2015-05-08T01:12:19Z
discontinued: false
dominant: false
experience: experience/Fails
group u list: _517_
m user id: 0
owner id: 6670
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is the tightly regulated lac operon BBa_K389050 with a mRFP gene as readout.
mutable notes: lacIq integrated in promoter so good regulation, mRFP as readout
mutable provenance: partsregistry
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0034
component: BBa_K389050
component: BBa_E1010
sequence annotation: BBa_K389050
sequence annotation: BBa_E1010
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K389052_sequence/1

BBa_B0034
type: Component
persistent identity: component2273042
display id: component2273042
version: 1
top level: BBa_K389052
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K389050
type: Component
persistent identity: component2273040
display id: component2273040
version: 1
top level: BBa_K389052
access: public
definition: https://synbiohub.org/public/igem/BBa_K389050/1

BBa_E1010
type: Component
persistent identity: component2273045
display id: component2273045
version: 1
top level: BBa_K389052
access: public
definition: mRFP1

BBa_K389050
type: SequenceAnnotation
persistent identity: annotation2273040
display id: annotation2273040
version: 1
top level: BBa_K389052
location: https://synbiohub.org/public/igem/BBa_K389052/annotation2273040/range2273040/1
component: BBa_K389050

https://synbiohub.org/public/igem/BBa_K389052/annotation2273040/range2273040/1
type: Range
persistent identity: range2273040
display id: range2273040
version: 1
direction: direction/reverse
top level: BBa_K389052
start: 1
end: 1600
orientation: inline

BBa_E1010
type: SequenceAnnotation
persistent identity: annotation2273045
display id: annotation2273045
version: 1
top level: BBa_K389052
location: https://synbiohub.org/public/igem/BBa_K389052/annotation2273045/range2273045/1
component: BBa_E1010

https://synbiohub.org/public/igem/BBa_K389052/annotation2273045/range2273045/1
type: Range
persistent identity: range2273045
display id: range2273045
version: 1
direction: direction/reverse
top level: BBa_K389052
start: 1627
end: 2332
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2273042
display id: annotation2273042
version: 1
top level: BBa_K389052
location: https://synbiohub.org/public/igem/BBa_K389052/annotation2273042/range2273042/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K389052/annotation2273042/range2273042/1
type: Range
persistent identity: range2273042
display id: range2273042
version: 1
direction: direction/reverse
top level: BBa_K389052
start: 1609
end: 1620
orientation: inline

BBa_K1486015
type: ComponentDefinition
persistent identity: BBa K1486015
display id: BBa_K1486015
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: IFP[2]-CpxR under pBAD
creator: EPFL iGem team 2014
created: 2014-09-09T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part has a fused protein downstream of an arabinose-inducible promoter: IFP[2]-CpxR. Biobrick BBa_K1486014 has IFP[1]-CpxR with an arabinose promoter. This construct was made in both orientations: BBa_K1486013 is CpxR-IFP[2] (with an arabinose promoter).
mutable notes: Both orientations were made, as well as for IFP[1] so all dimerizations could be tested (see main page for biobrick numbers). Flexible linkers were used to fuse both proteins, and an RBS was put before each one of them.
mutable provenance: To edit.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0034
component: BBa_K1486000
component: BBa_I0500
component: BBa_K1486004
component: BBa_K1486007
sequence annotation: BBa_I0500
sequence annotation: BBa_K1486004
sequence annotation: BBa_B0034
sequence annotation: BBa_K1486000
sequence annotation: BBa_K1486007
sequence: https://synbiohub.org/public/igem/BBa_K1486015_sequence/1

BBa_B0034
type: Component
persistent identity: component2382850
display id: component2382850
version: 1
top level: BBa_K1486015
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K1486000
type: Component
persistent identity: component2382859
display id: component2382859
version: 1
top level: BBa_K1486015
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486000/1

BBa_I0500
type: Component
persistent identity: component2382848
display id: component2382848
version: 1
top level: BBa_K1486015
access: public
definition: https://synbiohub.org/public/igem/BBa_I0500/1

BBa_K1486004
type: Component
persistent identity: component2382855
display id: component2382855
version: 1
top level: BBa_K1486015
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486004/1

BBa_K1486007
type: Component
persistent identity: component2382854
display id: component2382854
version: 1
top level: BBa_K1486015
access: public
definition: BBa_K1486007

BBa_I0500
type: SequenceAnnotation
persistent identity: annotation2382848
display id: annotation2382848
version: 1
top level: BBa_K1486015
location: https://synbiohub.org/public/igem/BBa_K1486015/annotation2382848/range2382848/1
component: BBa_I0500

https://synbiohub.org/public/igem/BBa_K1486015/annotation2382848/range2382848/1
type: Range
persistent identity: range2382848
display id: range2382848
version: 1
direction: direction/reverse
top level: BBa_K1486015
start: 1
end: 1210
orientation: inline

BBa_K1486004
type: SequenceAnnotation
persistent identity: annotation2382855
display id: annotation2382855
version: 1
top level: BBa_K1486015
location: https://synbiohub.org/public/igem/BBa_K1486015/annotation2382855/range2382855/1
component: BBa_K1486004

https://synbiohub.org/public/igem/BBa_K1486015/annotation2382855/range2382855/1
type: Range
persistent identity: range2382855
display id: range2382855
version: 1
direction: direction/reverse
top level: BBa_K1486015
start: 1824
end: 1853
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2382850
display id: annotation2382850
version: 1
top level: BBa_K1486015
location: https://synbiohub.org/public/igem/BBa_K1486015/annotation2382850/range2382850/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1486015/annotation2382850/range2382850/1
type: Range
persistent identity: range2382850
display id: range2382850
version: 1
direction: direction/reverse
top level: BBa_K1486015
start: 1219
end: 1230
orientation: inline

BBa_K1486000
type: SequenceAnnotation
persistent identity: annotation2382859
display id: annotation2382859
version: 1
top level: BBa_K1486015
location: https://synbiohub.org/public/igem/BBa_K1486015/annotation2382859/range2382859/1
component: BBa_K1486000

https://synbiohub.org/public/igem/BBa_K1486015/annotation2382859/range2382859/1
type: Range
persistent identity: range2382859
display id: range2382859
version: 1
direction: direction/reverse
top level: BBa_K1486015
start: 1860
end: 2561
orientation: inline

BBa_K1486007
type: SequenceAnnotation
persistent identity: annotation2382854
display id: annotation2382854
version: 1
top level: BBa_K1486015
location: https://synbiohub.org/public/igem/BBa_K1486015/annotation2382854/range2382854/1
component: BBa_K1486007

https://synbiohub.org/public/igem/BBa_K1486015/annotation2382854/range2382854/1
type: Range
persistent identity: range2382854
display id: range2382854
version: 1
direction: direction/reverse
top level: BBa_K1486015
start: 1237
end: 1815
orientation: inline

CBDclos(J6
type: ComponentDefinition
persistent identity: BBa K863114
display id: BBa_K863114
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CBD of C. cellulovorans cellulose binding protein gene with const. Promoter (J23100+J61101) and GFP
creator: Moritz M??ller
created: 2012-09-22T11:00:00Z
modified: 2015-06-17T01:20:44Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1123_
m user id: 4206
owner id: 13080
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Cellulose binding Domain of Clostridium cellulovorans [http://www.ncbi.nlm.nih.gov/nuccore/M73817 cellulose binding protein gene (cbp A)] with a constitutive promoter and RBS ( J61101 ) and a fused-GFP protein with His-tag.
mutable notes: Was made to switch from a T7-promoter to a constitutive promoter
mutable provenance: Cellulose binding domain form a cloning-vector. J61101 and [http://partsregistry.org/Part:BBa_I13522 GFP] from the partsregistry.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: BBa_K863121
sequence annotation: Freiburg-Scar
sequence annotation: TAA
sequence annotation: NgoMIV
sequence annotation: Cellulose binding domain (CBDclos)
sequence annotation: AgeI
sequence annotation: ATG
sequence annotation: Cellulose Binding Protein
sequence annotation: BBa_J61101
sequence annotation: BBa_J23100
sequence annotation: His-Tag
sequence annotation: BBa_K863111
sequence annotation: BBa_E0040
sequence annotation: GS-Linker
sequence: https://synbiohub.org/public/igem/BBa_K863114_sequence/1

BBa_K863121
type: SequenceAnnotation
persistent identity: annotation2194139
display id: annotation2194139
version: 1
top level: CBDclos(J6
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K863114/annotation2194139/range2194139/1

https://synbiohub.org/public/igem/BBa_K863114/annotation2194139/range2194139/1
type: Range
persistent identity: range2194139
display id: range2194139
version: 1
direction: direction/reverse
top level: CBDclos(J6
start: 377
end: 1117
orientation: inline

Freiburg-Scar
type: SequenceAnnotation
persistent identity: annotation2194138
display id: annotation2194138
version: 1
top level: CBDclos(J6
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K863114/annotation2194138/range2194138/1

https://synbiohub.org/public/igem/BBa_K863114/annotation2194138/range2194138/1
type: Range
persistent identity: range2194138
display id: range2194138
version: 1
direction: direction/forward
top level: CBDclos(J6
start: 371
end: 376
orientation: inline

TAA
type: SequenceAnnotation
persistent identity: annotation2194143
display id: annotation2194143
version: 1
top level: CBDclos(J6
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K863114/annotation2194143/range2194143/1

https://synbiohub.org/public/igem/BBa_K863114/annotation2194143/range2194143/1
type: Range
persistent identity: range2194143
display id: range2194143
version: 1
direction: direction/forward
top level: CBDclos(J6
start: 1115
end: 1117
orientation: inline

NgoMIV
type: SequenceAnnotation
persistent identity: annotation2194133
display id: annotation2194133
version: 1
top level: CBDclos(J6
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K863114/annotation2194133/range2194133/1

https://synbiohub.org/public/igem/BBa_K863114/annotation2194133/range2194133/1
type: Range
persistent identity: range2194133
display id: range2194133
version: 1
direction: direction/forward
top level: CBDclos(J6
start: 65
end: 70
orientation: inline

Cellulose binding domain (CBDclos)
type: SequenceAnnotation
persistent identity: annotation2194135
display id: annotation2194135
version: 1
top level: CBDclos(J6
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K863114/annotation2194135/range2194135/1

https://synbiohub.org/public/igem/BBa_K863114/annotation2194135/range2194135/1
type: Range
persistent identity: range2194135
display id: range2194135
version: 1
direction: direction/forward
top level: CBDclos(J6
start: 83
end: 358
orientation: inline

AgeI
type: SequenceAnnotation
persistent identity: annotation2194142
display id: annotation2194142
version: 1
top level: CBDclos(J6
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K863114/annotation2194142/range2194142/1

https://synbiohub.org/public/igem/BBa_K863114/annotation2194142/range2194142/1
type: Range
persistent identity: range2194142
display id: range2194142
version: 1
direction: direction/forward
top level: CBDclos(J6
start: 1109
end: 1114
orientation: inline

ATG
type: SequenceAnnotation
persistent identity: annotation2194132
display id: annotation2194132
version: 1
top level: CBDclos(J6
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K863114/annotation2194132/range2194132/1

https://synbiohub.org/public/igem/BBa_K863114/annotation2194132/range2194132/1
type: Range
persistent identity: range2194132
display id: range2194132
version: 1
direction: direction/forward
top level: CBDclos(J6
start: 62
end: 64
orientation: inline

Cellulose Binding Protein
type: SequenceAnnotation
persistent identity: annotation2194136
display id: annotation2194136
version: 1
top level: CBDclos(J6
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K863114/annotation2194136/range2194136/1

https://synbiohub.org/public/igem/BBa_K863114/annotation2194136/range2194136/1
type: Range
persistent identity: range2194136
display id: range2194136
version: 1
direction: direction/forward
top level: CBDclos(J6
start: 359
end: 364
orientation: inline

BBa_J61101
type: SequenceAnnotation
persistent identity: annotation2194130
display id: annotation2194130
version: 1
top level: CBDclos(J6
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K863114/annotation2194130/range2194130/1

https://synbiohub.org/public/igem/BBa_K863114/annotation2194130/range2194130/1
type: Range
persistent identity: range2194130
display id: range2194130
version: 1
direction: direction/reverse
top level: CBDclos(J6
start: 44
end: 55
orientation: inline

BBa_J23100
type: SequenceAnnotation
persistent identity: annotation2194129
display id: annotation2194129
version: 1
top level: CBDclos(J6
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K863114/annotation2194129/range2194129/1

https://synbiohub.org/public/igem/BBa_K863114/annotation2194129/range2194129/1
type: Range
persistent identity: range2194129
display id: range2194129
version: 1
direction: direction/reverse
top level: CBDclos(J6
start: 1
end: 35
orientation: inline

His-Tag
type: SequenceAnnotation
persistent identity: annotation2194141
display id: annotation2194141
version: 1
top level: CBDclos(J6
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K863114/annotation2194141/range2194141/1

https://synbiohub.org/public/igem/BBa_K863114/annotation2194141/range2194141/1
type: Range
persistent identity: range2194141
display id: range2194141
version: 1
direction: direction/forward
top level: CBDclos(J6
start: 1091
end: 1108
orientation: inline

BBa_K863111
type: SequenceAnnotation
persistent identity: annotation2194131
display id: annotation2194131
version: 1
top level: CBDclos(J6
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K863114/annotation2194131/range2194131/1

https://synbiohub.org/public/igem/BBa_K863114/annotation2194131/range2194131/1
type: Range
persistent identity: range2194131
display id: range2194131
version: 1
direction: direction/reverse
top level: CBDclos(J6
start: 62
end: 370
orientation: inline

Cellulose Binding Protein
type: SequenceAnnotation
persistent identity: annotation2194134
display id: annotation2194134
version: 1
top level: CBDclos(J6
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K863114/annotation2194134/range2194134/1

https://synbiohub.org/public/igem/BBa_K863114/annotation2194134/range2194134/1
type: Range
persistent identity: range2194134
display id: range2194134
version: 1
direction: direction/forward
top level: CBDclos(J6
start: 71
end: 82
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation2194140
display id: annotation2194140
version: 1
top level: CBDclos(J6
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K863114/annotation2194140/range2194140/1

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type: Range
persistent identity: range2194140
display id: range2194140
version: 1
direction: direction/forward
top level: CBDclos(J6
start: 377
end: 1090
orientation: inline

GS-Linker
type: SequenceAnnotation
persistent identity: annotation2194137
display id: annotation2194137
version: 1
top level: CBDclos(J6
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K863114/annotation2194137/range2194137/1

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type: Range
persistent identity: range2194137
display id: range2194137
version: 1
direction: direction/forward
top level: CBDclos(J6
start: 365
end: 370
orientation: inline

BBa_K1031803
type: ComponentDefinition
persistent identity: BBa K1031803
display id: BBa_K1031803
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Pu-B0031-sfGFP-Terminator (XylR)
creator: He Shuaixin
created: 2013-09-08T11:00:00Z
modified: 2015-05-08T01:08:48Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1338_
m user id: 0
owner id: 15843
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Pu-31-sfGFP-TT (XylR)
mutable notes: molecular cloning
mutable provenance: synthesis, lab made
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: B0031
sequence annotation: Pu (XylR)
sequence annotation: sfGFP
sequence annotation: B0015
sequence: https://synbiohub.org/public/igem/BBa_K1031803_sequence/1

B0031
type: SequenceAnnotation
persistent identity: annotation2362526
display id: annotation2362526
version: 1
top level: BBa_K1031803
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1031803/annotation2362526/range2362526/1

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type: Range
persistent identity: range2362526
display id: range2362526
version: 1
direction: direction/forward
top level: BBa_K1031803
start: 187
end: 200
orientation: inline

Pu (XylR)
type: SequenceAnnotation
persistent identity: annotation2362525
display id: annotation2362525
version: 1
top level: BBa_K1031803
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1031803/annotation2362525/range2362525/1

https://synbiohub.org/public/igem/BBa_K1031803/annotation2362525/range2362525/1
type: Range
persistent identity: range2362525
display id: range2362525
version: 1
direction: direction/forward
top level: BBa_K1031803
start: 1
end: 180
orientation: inline

sfGFP
type: SequenceAnnotation
persistent identity: annotation2362527
display id: annotation2362527
version: 1
top level: BBa_K1031803
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1031803/annotation2362527/range2362527/1

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type: Range
persistent identity: range2362527
display id: range2362527
version: 1
direction: direction/forward
top level: BBa_K1031803
start: 221
end: 929
orientation: inline

B0015
type: SequenceAnnotation
persistent identity: annotation2362528
display id: annotation2362528
version: 1
top level: BBa_K1031803
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1031803/annotation2362528/range2362528/1

https://synbiohub.org/public/igem/BBa_K1031803/annotation2362528/range2362528/1
type: Range
persistent identity: range2362528
display id: range2362528
version: 1
direction: direction/forward
top level: BBa_K1031803
start: 936
end: 1064
orientation: inline

GFP
type: ComponentDefinition
persistent identity: BBa K678027
display id: BBa_K678027
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: GFP for Aspergillus nidulans
creator: DTU-Denmark-2
created: 2011-09-10T11:00:00Z
modified: 2015-05-08T01:13:04Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _882_
m user id: 0
owner id: 8341
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: GFP for Aspergillus nidulans
mutable notes: Design considerations This part was designed for the Plug'n'Play with DNA assembly standard (RFC 80), therefore this part contains linkers at the 5' and 3' ends as shown on the figure below.
mutable provenance: to be announced
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: Linker 2
sequence annotation: GFP
sequence: https://synbiohub.org/public/igem/BBa_K678027_sequence/1

Linker 2
type: SequenceAnnotation
persistent identity: annotation2127382
display id: annotation2127382
version: 1
top level: GFP
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678027/annotation2127382/range2127382/1

https://synbiohub.org/public/igem/BBa_K678027/annotation2127382/range2127382/1
type: Range
persistent identity: range2127382
display id: range2127382
version: 1
direction: direction/forward
top level: GFP
start: 1
end: 8
orientation: inline

GFP
type: SequenceAnnotation
persistent identity: annotation2127383
display id: annotation2127383
version: 1
top level: GFP
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K678027/annotation2127383/range2127383/1

https://synbiohub.org/public/igem/BBa_K678027/annotation2127383/range2127383/1
type: Range
persistent identity: range2127383
display id: range2127383
version: 1
direction: direction/forward
top level: GFP
start: 9
end: 728
orientation: inline

BBa_K772103
type: ComponentDefinition
persistent identity: BBa K772103
display id: BBa_K772103
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: GFP - LacZ protein complex generator
creator: Mustafa Elitok
created: 2012-09-25T11:00:00Z
modified: 2015-05-08T01:13:15Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1024_
m user id: 0
owner id: 9573
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: GFP - LacZ protein generator is generated with the aim of inducing a reporting reaction aftermath of the activation of EpCAM detection system. (BBa_K772100) For more information: BBa_K772006
mutable notes: To achieve a constitutive production, pTetR promoter is added in the upstream region of the original part.
mutable provenance: Cleavage site, LacZ and GFP sequences are gathered from Registry.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_R0040
component: BBa_K772006
sequence annotation: BBa_R0040
sequence annotation: BBa_K772006
sequence: https://synbiohub.org/public/igem/BBa_K772103_sequence/1

BBa_R0040
type: Component
persistent identity: component2211349
display id: component2211349
version: 1
top level: BBa_K772103
access: public
definition: https://synbiohub.org/public/igem/BBa_R0040/1

BBa_K772006
type: Component
persistent identity: component2211360
display id: component2211360
version: 1
top level: BBa_K772103
access: public
definition: AnRe

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2211349
display id: annotation2211349
version: 1
top level: BBa_K772103
location: https://synbiohub.org/public/igem/BBa_K772103/annotation2211349/range2211349/1
component: BBa_R0040

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type: Range
persistent identity: range2211349
display id: range2211349
version: 1
direction: direction/forward
top level: BBa_K772103
start: 1
end: 54
orientation: inline

BBa_K772006
type: SequenceAnnotation
persistent identity: annotation2211360
display id: annotation2211360
version: 1
top level: BBa_K772103
location: https://synbiohub.org/public/igem/BBa_K772103/annotation2211360/range2211360/1
component: BBa_K772006

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type: Range
persistent identity: range2211360
display id: range2211360
version: 1
direction: direction/reverse
top level: BBa_K772103
start: 63
end: 1269
orientation: inline

BBa_K1861030
type: ComponentDefinition
persistent identity: BBa K1861030
display id: BBa_K1861030
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: eYFP gene for expression in yeast
creator: Vladyslav Vyshnevskyi
created: 2015-09-17T11:00:00Z
modified: 2015-09-23T04:49:52Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2291_
m user id: 25348
owner id: 25348
owning group id: 9
status: status/Planning
bookmark: true
mutable description: Coming soon.
mutable notes: Coming soon.
mutable provenance: Coming soon.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_J63002
component: BBa_K1861301
component: BBa_K1861303
component: BBa_K1861304
component: BBa_K792001
component: BBa_K1861302
sequence annotation: BBa_K792001
sequence annotation: BBa_K1861304
sequence annotation: BBa_K1861301
sequence annotation: BBa_K1861303
sequence annotation: BBa_K1861302
sequence annotation: BBa_J63002
sequence: https://synbiohub.org/public/igem/BBa_K1861030_sequence/1

BBa_J63002
type: Component
persistent identity: component2474915
display id: component2474915
version: 1
top level: BBa_K1861030
access: public
definition: https://synbiohub.org/public/igem/BBa_J63002/1

BBa_K1861301
type: Component
persistent identity: component2474907
display id: component2474907
version: 1
top level: BBa_K1861030
access: public
definition: https://synbiohub.org/public/igem/BBa_K1861301/1

BBa_K1861303
type: Component
persistent identity: component2474908
display id: component2474908
version: 1
top level: BBa_K1861030
access: public
definition: https://synbiohub.org/public/igem/BBa_K1861303/1

BBa_K1861304
type: Component
persistent identity: component2474916
display id: component2474916
version: 1
top level: BBa_K1861030
access: public
definition: https://synbiohub.org/public/igem/BBa_K1861304/1

BBa_K792001
type: Component
persistent identity: component2474912
display id: component2474912
version: 1
top level: BBa_K1861030
access: public
definition: https://synbiohub.org/public/igem/BBa_K792001/1

BBa_K1861302
type: Component
persistent identity: component2474913
display id: component2474913
version: 1
top level: BBa_K1861030
access: public
definition: https://synbiohub.org/public/igem/BBa_K1861302/1

BBa_K792001
type: SequenceAnnotation
persistent identity: annotation2474912
display id: annotation2474912
version: 1
top level: BBa_K1861030
location: https://synbiohub.org/public/igem/BBa_K1861030/annotation2474912/range2474912/1
component: BBa_K792001

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type: Range
persistent identity: range2474912
display id: range2474912
version: 1
direction: direction/reverse
top level: BBa_K1861030
start: 276
end: 296
orientation: inline

BBa_K1861304
type: SequenceAnnotation
persistent identity: annotation2474916
display id: annotation2474916
version: 1
top level: BBa_K1861030
location: https://synbiohub.org/public/igem/BBa_K1861030/annotation2474916/range2474916/1
component: BBa_K1861304

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type: Range
persistent identity: range2474916
display id: range2474916
version: 1
direction: direction/reverse
top level: BBa_K1861030
start: 1287
end: 1349
orientation: inline

BBa_K1861301
type: SequenceAnnotation
persistent identity: annotation2474907
display id: annotation2474907
version: 1
top level: BBa_K1861030
location: https://synbiohub.org/public/igem/BBa_K1861030/annotation2474907/range2474907/1
component: BBa_K1861301

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type: Range
persistent identity: range2474907
display id: range2474907
version: 1
direction: direction/reverse
top level: BBa_K1861030
start: 1
end: 71
orientation: inline

BBa_K1861303
type: SequenceAnnotation
persistent identity: annotation2474908
display id: annotation2474908
version: 1
top level: BBa_K1861030
location: https://synbiohub.org/public/igem/BBa_K1861030/annotation2474908/range2474908/1
component: BBa_K1861303

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type: Range
persistent identity: range2474908
display id: range2474908
version: 1
direction: direction/reverse
top level: BBa_K1861030
start: 72
end: 275
orientation: inline

BBa_K1861302
type: SequenceAnnotation
persistent identity: annotation2474913
display id: annotation2474913
version: 1
top level: BBa_K1861030
location: https://synbiohub.org/public/igem/BBa_K1861030/annotation2474913/range2474913/1
component: BBa_K1861302

https://synbiohub.org/public/igem/BBa_K1861030/annotation2474913/range2474913/1
type: Range
persistent identity: range2474913
display id: range2474913
version: 1
direction: direction/reverse
top level: BBa_K1861030
start: 297
end: 1061
orientation: inline

BBa_J63002
type: SequenceAnnotation
persistent identity: annotation2474915
display id: annotation2474915
version: 1
top level: BBa_K1861030
location: https://synbiohub.org/public/igem/BBa_K1861030/annotation2474915/range2474915/1
component: BBa_J63002

https://synbiohub.org/public/igem/BBa_K1861030/annotation2474915/range2474915/1
type: Range
persistent identity: range2474915
display id: range2474915
version: 1
direction: direction/reverse
top level: BBa_K1861030
start: 1062
end: 1286
orientation: inline

mOrange
type: ComponentDefinition
persistent identity: BBa K105011
display id: BBa_K105011
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: mOrange, Yeast optimized for fusion proteins
creator: Marius M??ller, Paul Bourgine
created: 2008-10-11T11:00:00Z
modified: 2015-05-08T01:08:52Z
discontinued: false
dominant: false
experience: experience/None
group u list: _253_
m user id: 0
owner id: 3313
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Our BioBrick is red-orange fluorescent protein which can be used to label proteins in yeast. Therefor it has specific characteristics of a BioBrick part for fusion proteins (see design details). <br\n>
mutable notes: To enable the fusion with other domains in frame the vector of this BioBrick has no base pair in between the restriction side and the BioBrick. Furthermore, this coding sequence does not include a start codon.<br\n> For more information about this issus, see:<br\n> Phillips, I.E. and Silver, P.A. "A new Biobrick Assembly Strategy Designed for Facile Protein Engineering." <br\n> DSpace http://hdl.handle.net/1721.1/32535 (2006).
mutable provenance: We changed the format of BBa_E2050 into Phillips/Silver standard.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence: https://synbiohub.org/public/igem/BBa_K105011_sequence/1

BBa_K1486008
type: ComponentDefinition
persistent identity: BBa K1486008
display id: BBa_K1486008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CxpR & Split IFP1.4 [Cterm + Cterm][1]
creator: iGem EPFL 2014
created: 2014-08-25T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part has two fused proteins downstream of an arabinose-inducible promoter: CpxR-IFP[1] and CpxR-IFP[2]. This construct was made in all orientations: - IFP[1]-CpxR + IFP[2]-CpxR (BBa_K1286009) - IFP[1]-CpxR + CpxR-IFP[2] (BBa_K1486010) - CpxR-IFP[1] + IFP[2]-CpxR (BBa_K1486011)
mutable notes: All orientations were made, so all dimerizations could be tested (see main page for biobrick numbers). Flexible linkers were used for each of the fused proteins, and an RBS was put before each one of them.
mutable provenance: From Michnick Article. To Edit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0034
component: BBa_K1486004
component: BBa_K1486000
component: BBa_I0500
component: BBa_K1486006
component: BBa_K1486052
component: BBa_K1486007
sequence annotation: BBa_K1486004
sequence annotation: BBa_B0034
sequence annotation: BBa_I0500
sequence annotation: BBa_K1486007
sequence annotation: BBa_K1486006
sequence annotation: BBa_K1486052
sequence annotation: BBa_K1486000
sequence: https://synbiohub.org/public/igem/BBa_K1486008_sequence/1

BBa_B0034
type: Component
persistent identity: component2418036
display id: component2418036
version: 1
top level: BBa_K1486008
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K1486004
type: Component
persistent identity: component2418053
display id: component2418053
version: 1
top level: BBa_K1486008
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486004/1

BBa_K1486000
type: Component
persistent identity: component2418040
display id: component2418040
version: 1
top level: BBa_K1486008
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486000/1

BBa_I0500
type: Component
persistent identity: component2418034
display id: component2418034
version: 1
top level: BBa_K1486008
access: public
definition: https://synbiohub.org/public/igem/BBa_I0500/1

BBa_B0034
type: Component
persistent identity: component2418048
display id: component2418048
version: 1
top level: BBa_K1486008
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K1486000
type: Component
persistent identity: component2418052
display id: component2418052
version: 1
top level: BBa_K1486008
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486000/1

BBa_K1486006
type: Component
persistent identity: component2418045
display id: component2418045
version: 1
top level: BBa_K1486008
access: public
definition: BBa_K1486006

BBa_K1486004
type: Component
persistent identity: component2418041
display id: component2418041
version: 1
top level: BBa_K1486008
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486004/1

BBa_K1486052
type: Component
persistent identity: component2418046
display id: component2418046
version: 1
top level: BBa_K1486008
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486052/1

BBa_K1486007
type: Component
persistent identity: component2418057
display id: component2418057
version: 1
top level: BBa_K1486008
access: public
definition: BBa_K1486007

BBa_K1486004
type: SequenceAnnotation
persistent identity: annotation2418053
display id: annotation2418053
version: 1
top level: BBa_K1486008
location: https://synbiohub.org/public/igem/BBa_K1486008/annotation2418053/range2418053/1
component: BBa_K1486004

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type: Range
persistent identity: range2418053
display id: range2418053
version: 1
direction: direction/reverse
top level: BBa_K1486008
start: 3112
end: 3141
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2418036
display id: annotation2418036
version: 1
top level: BBa_K1486008
location: https://synbiohub.org/public/igem/BBa_K1486008/annotation2418036/range2418036/1
component: BBa_B0034

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type: Range
persistent identity: range2418036
display id: range2418036
version: 1
direction: direction/reverse
top level: BBa_K1486008
start: 1211
end: 1222
orientation: inline

BBa_K1486004
type: SequenceAnnotation
persistent identity: annotation2418041
display id: annotation2418041
version: 1
top level: BBa_K1486008
location: https://synbiohub.org/public/igem/BBa_K1486008/annotation2418041/range2418041/1
component: BBa_K1486004

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type: Range
persistent identity: range2418041
display id: range2418041
version: 1
direction: direction/reverse
top level: BBa_K1486008
start: 1925
end: 1954
orientation: inline

BBa_I0500
type: SequenceAnnotation
persistent identity: annotation2418034
display id: annotation2418034
version: 1
top level: BBa_K1486008
location: https://synbiohub.org/public/igem/BBa_K1486008/annotation2418034/range2418034/1
component: BBa_I0500

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type: Range
persistent identity: range2418034
display id: range2418034
version: 1
direction: direction/reverse
top level: BBa_K1486008
start: 1
end: 1210
orientation: inline

BBa_K1486007
type: SequenceAnnotation
persistent identity: annotation2418057
display id: annotation2418057
version: 1
top level: BBa_K1486008
location: https://synbiohub.org/public/igem/BBa_K1486008/annotation2418057/range2418057/1
component: BBa_K1486007

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type: Range
persistent identity: range2418057
display id: range2418057
version: 1
direction: direction/reverse
top level: BBa_K1486008
start: 3142
end: 3726
orientation: inline

BBa_K1486006
type: SequenceAnnotation
persistent identity: annotation2418045
display id: annotation2418045
version: 1
top level: BBa_K1486008
location: https://synbiohub.org/public/igem/BBa_K1486008/annotation2418045/range2418045/1
component: BBa_K1486006

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type: Range
persistent identity: range2418045
display id: range2418045
version: 1
direction: direction/reverse
top level: BBa_K1486008
start: 1955
end: 2359
orientation: inline

BBa_K1486052
type: SequenceAnnotation
persistent identity: annotation2418046
display id: annotation2418046
version: 1
top level: BBa_K1486008
location: https://synbiohub.org/public/igem/BBa_K1486008/annotation2418046/range2418046/1
component: BBa_K1486052

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type: Range
persistent identity: range2418046
display id: range2418046
version: 1
direction: direction/reverse
top level: BBa_K1486008
start: 2360
end: 2397
orientation: inline

BBa_K1486000
type: SequenceAnnotation
persistent identity: annotation2418052
display id: annotation2418052
version: 1
top level: BBa_K1486008
location: https://synbiohub.org/public/igem/BBa_K1486008/annotation2418052/range2418052/1
component: BBa_K1486000

https://synbiohub.org/public/igem/BBa_K1486008/annotation2418052/range2418052/1
type: Range
persistent identity: range2418052
display id: range2418052
version: 1
direction: direction/reverse
top level: BBa_K1486008
start: 2410
end: 3111
orientation: inline

BBa_K1486000
type: SequenceAnnotation
persistent identity: annotation2418040
display id: annotation2418040
version: 1
top level: BBa_K1486008
location: https://synbiohub.org/public/igem/BBa_K1486008/annotation2418040/range2418040/1
component: BBa_K1486000

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type: Range
persistent identity: range2418040
display id: range2418040
version: 1
direction: direction/reverse
top level: BBa_K1486008
start: 1223
end: 1924
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2418048
display id: annotation2418048
version: 1
top level: BBa_K1486008
location: https://synbiohub.org/public/igem/BBa_K1486008/annotation2418048/range2418048/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1486008/annotation2418048/range2418048/1
type: Range
persistent identity: range2418048
display id: range2418048
version: 1
direction: direction/reverse
top level: BBa_K1486008
start: 2398
end: 2409
orientation: inline

GFP
type: ComponentDefinition
persistent identity: BBa K145015
display id: BBa_K145015
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: GFP with LVA tag
creator: Benjamien Moeyaert
created: 2008-07-31T11:00:00Z
modified: 2015-05-08T01:10:28Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _257_
m user id: 0
owner id: 2939
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: Green Fluorescent Protein with LVA tag for rapid degradation
mutable notes: Designed out of pJBA111 (Molin, 1998) with PCR to add appropriote prefix and suffix.
mutable provenance: pJBA111, carrying GFP-LVA, was generously provided by Dr. S. Molin, The Technical University of Denmark, Lyngby Appl Environ Microbiol. 1998 Jun;64(6):2240-6. New unstable variants of green fluorescent protein for studies of transient gene expression in bacteria. Andersen JB, Sternberg C, Poulsen LK, Bjorn SP, Givskov M, Molin S. PMID: 9603842
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: start
sequence annotation: LVA tag
sequence annotation: cds
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_K145015_sequence/1

start
type: SequenceAnnotation
persistent identity: annotation1969616
display id: annotation1969616
version: 1
top level: GFP
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K145015/annotation1969616/range1969616/1

https://synbiohub.org/public/igem/BBa_K145015/annotation1969616/range1969616/1
type: Range
persistent identity: range1969616
display id: range1969616
version: 1
direction: direction/forward
top level: GFP
start: 1
end: 3
orientation: inline

LVA tag
type: SequenceAnnotation
persistent identity: annotation1969619
display id: annotation1969619
version: 1
top level: GFP
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K145015/annotation1969619/range1969619/1

https://synbiohub.org/public/igem/BBa_K145015/annotation1969619/range1969619/1
type: Range
persistent identity: range1969619
display id: range1969619
version: 1
direction: direction/forward
top level: GFP
start: 717
end: 753
orientation: inline

cds
type: SequenceAnnotation
persistent identity: annotation1969620
display id: annotation1969620
version: 1
top level: GFP
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K145015/annotation1969620/range1969620/1

https://synbiohub.org/public/igem/BBa_K145015/annotation1969620/range1969620/1
type: Range
persistent identity: range1969620
display id: range1969620
version: 1
direction: direction/forward
top level: GFP
start: 4
end: 716
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1969617
display id: annotation1969617
version: 1
top level: GFP
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K145015/annotation1969617/range1969617/1

https://synbiohub.org/public/igem/BBa_K145015/annotation1969617/range1969617/1
type: Range
persistent identity: range1969617
display id: range1969617
version: 1
direction: direction/forward
top level: GFP
start: 754
end: 756
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1969618
display id: annotation1969618
version: 1
top level: GFP
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K145015/annotation1969618/range1969618/1

https://synbiohub.org/public/igem/BBa_K145015/annotation1969618/range1969618/1
type: Range
persistent identity: range1969618
display id: range1969618
version: 1
direction: direction/forward
top level: GFP
start: 757
end: 759
orientation: inline

BBa_K1486041
type: ComponentDefinition
persistent identity: BBa K1486041
display id: BBa_K1486041
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: sfGFPC + CpxR
creator: Ione Pla Puigsubir??
created: 2014-09-26T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: To edit
mutable notes: To edit
mutable provenance: To edit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_I0500
component: BBa_K1486000
component: BBa_K1486037
component: BBa_B0034
component: BBa_K1486039
sequence annotation: BBa_K1486037
sequence annotation: BBa_K1486000
sequence annotation: BBa_B0034
sequence annotation: BBa_I0500
sequence annotation: BBa_K1486039
sequence: https://synbiohub.org/public/igem/BBa_K1486041_sequence/1

BBa_I0500
type: Component
persistent identity: component2387209
display id: component2387209
version: 1
top level: BBa_K1486041
access: public
definition: https://synbiohub.org/public/igem/BBa_I0500/1

BBa_K1486000
type: Component
persistent identity: component2387217
display id: component2387217
version: 1
top level: BBa_K1486041
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486000/1

BBa_K1486037
type: Component
persistent identity: component2387213
display id: component2387213
version: 1
top level: BBa_K1486041
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486037/1

BBa_B0034
type: Component
persistent identity: component2387211
display id: component2387211
version: 1
top level: BBa_K1486041
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K1486039
type: Component
persistent identity: component2387212
display id: component2387212
version: 1
top level: BBa_K1486041
access: public
definition: BBa_K1486039

BBa_K1486037
type: SequenceAnnotation
persistent identity: annotation2387213
display id: annotation2387213
version: 1
top level: BBa_K1486041
location: https://synbiohub.org/public/igem/BBa_K1486041/annotation2387213/range2387213/1
component: BBa_K1486037

https://synbiohub.org/public/igem/BBa_K1486041/annotation2387213/range2387213/1
type: Range
persistent identity: range2387213
display id: range2387213
version: 1
direction: direction/reverse
top level: BBa_K1486041
start: 1295
end: 1333
orientation: inline

BBa_K1486000
type: SequenceAnnotation
persistent identity: annotation2387217
display id: annotation2387217
version: 1
top level: BBa_K1486041
location: https://synbiohub.org/public/igem/BBa_K1486041/annotation2387217/range2387217/1
component: BBa_K1486000

https://synbiohub.org/public/igem/BBa_K1486041/annotation2387217/range2387217/1
type: Range
persistent identity: range2387217
display id: range2387217
version: 1
direction: direction/reverse
top level: BBa_K1486041
start: 1334
end: 2035
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2387211
display id: annotation2387211
version: 1
top level: BBa_K1486041
location: https://synbiohub.org/public/igem/BBa_K1486041/annotation2387211/range2387211/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1486041/annotation2387211/range2387211/1
type: Range
persistent identity: range2387211
display id: range2387211
version: 1
direction: direction/reverse
top level: BBa_K1486041
start: 1211
end: 1222
orientation: inline

BBa_I0500
type: SequenceAnnotation
persistent identity: annotation2387209
display id: annotation2387209
version: 1
top level: BBa_K1486041
location: https://synbiohub.org/public/igem/BBa_K1486041/annotation2387209/range2387209/1
component: BBa_I0500

https://synbiohub.org/public/igem/BBa_K1486041/annotation2387209/range2387209/1
type: Range
persistent identity: range2387209
display id: range2387209
version: 1
direction: direction/reverse
top level: BBa_K1486041
start: 1
end: 1210
orientation: inline

BBa_K1486039
type: SequenceAnnotation
persistent identity: annotation2387212
display id: annotation2387212
version: 1
top level: BBa_K1486041
location: https://synbiohub.org/public/igem/BBa_K1486041/annotation2387212/range2387212/1
component: BBa_K1486039

https://synbiohub.org/public/igem/BBa_K1486041/annotation2387212/range2387212/1
type: Range
persistent identity: range2387212
display id: range2387212
version: 1
direction: direction/reverse
top level: BBa_K1486041
start: 1223
end: 1294
orientation: inline

BBa_K1680017
type: ComponentDefinition
persistent identity: BBa K1680017
display id: BBa_K1680017
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Cre-Dronpa Fusion
creator: Nicolai von K??gelgen
created: 2015-09-16T11:00:00Z
modified: 2016-01-26T10:15:17Z
discontinued: false
dominant: false
group u list: _2098_
m user id: 4206
owner id: 26366
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Protein coding region for a fusion construct of Cre Recombinase and 145N Dronpa with intermediate 6 amino acid linker. Oligomerisation of Dronpa should stop Cre from accessing DNA in the nucleus thereby inhibiting it until light deactivation of dronpa.
mutable notes: Assembled using 3A assembly.
mutable provenance: gBlocks designed by Team Tuebingen.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: BBa_K1680000
sequence annotation: BBa_K1680007
sequence annotation: BBa_K1680006
sequence: https://synbiohub.org/public/igem/BBa_K1680017_sequence/1

BBa_K1680000
type: SequenceAnnotation
persistent identity: annotation2465976
display id: annotation2465976
version: 1
top level: BBa_K1680017
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1680017/annotation2465976/range2465976/1

https://synbiohub.org/public/igem/BBa_K1680017/annotation2465976/range2465976/1
type: Range
persistent identity: range2465976
display id: range2465976
version: 1
direction: direction/reverse
top level: BBa_K1680017
start: 1
end: 6
orientation: inline

BBa_K1680007
type: SequenceAnnotation
persistent identity: annotation2465977
display id: annotation2465977
version: 1
top level: BBa_K1680017
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1680017/annotation2465977/range2465977/1

https://synbiohub.org/public/igem/BBa_K1680017/annotation2465977/range2465977/1
type: Range
persistent identity: range2465977
display id: range2465977
version: 1
direction: direction/reverse
top level: BBa_K1680017
start: 13
end: 1041
orientation: inline

BBa_K1680000
type: SequenceAnnotation
persistent identity: annotation2465978
display id: annotation2465978
version: 1
top level: BBa_K1680017
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1680017/annotation2465978/range2465978/1

https://synbiohub.org/public/igem/BBa_K1680017/annotation2465978/range2465978/1
type: Range
persistent identity: range2465978
display id: range2465978
version: 1
direction: direction/reverse
top level: BBa_K1680017
start: 1050
end: 1055
orientation: inline

BBa_K1680006
type: SequenceAnnotation
persistent identity: annotation2465979
display id: annotation2465979
version: 1
top level: BBa_K1680017
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1680017/annotation2465979/range2465979/1

https://synbiohub.org/public/igem/BBa_K1680017/annotation2465979/range2465979/1
type: Range
persistent identity: range2465979
display id: range2465979
version: 1
direction: direction/reverse
top level: BBa_K1680017
start: 1064
end: 1732
orientation: inline

ECFP
type: ComponentDefinition
persistent identity: BBa E0026
display id: BBa_E0026
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: enhanced cyan fluorescent protein derived from A. victoria GFP
creator: jcbraff
created: 2004-06-06T11:00:00Z
modified: 2015-08-31T04:07:25Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_1_
m user id: 0
owner id: 61
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence: https://synbiohub.org/public/igem/BBa_E0026_sequence/1

BBa_K1364015
type: ComponentDefinition
persistent identity: BBa K1364015
display id: BBa_K1364015
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Pveg + RFP
creator: Jourdan Camille
created: 2014-10-02T11:00:00Z
modified: 2015-06-08T09:04:37Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1740_
m user id: 4206
owner id: 21465
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: bla
mutable notes: bla
mutable provenance: bla
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_K606013
component: BBa_K823003
sequence annotation: BBa_K606013
sequence annotation: BBa_K823003
sequence: https://synbiohub.org/public/igem/BBa_K1364015_sequence/1

BBa_K606013
type: Component
persistent identity: component2392968
display id: component2392968
version: 1
top level: BBa_K1364015
access: public
definition: https://synbiohub.org/public/igem/BBa_K606013/1

BBa_K823003
type: Component
persistent identity: component2392954
display id: component2392954
version: 1
top level: BBa_K1364015
access: public
definition: https://synbiohub.org/public/igem/BBa_K823003/1

BBa_K606013
type: SequenceAnnotation
persistent identity: annotation2392968
display id: annotation2392968
version: 1
top level: BBa_K1364015
location: https://synbiohub.org/public/igem/BBa_K1364015/annotation2392968/range2392968/1
component: BBa_K606013

https://synbiohub.org/public/igem/BBa_K1364015/annotation2392968/range2392968/1
type: Range
persistent identity: range2392968
display id: range2392968
version: 1
direction: direction/reverse
top level: BBa_K1364015
start: 246
end: 1106
orientation: inline

BBa_K823003
type: SequenceAnnotation
persistent identity: annotation2392954
display id: annotation2392954
version: 1
top level: BBa_K1364015
location: https://synbiohub.org/public/igem/BBa_K1364015/annotation2392954/range2392954/1
component: BBa_K823003

https://synbiohub.org/public/igem/BBa_K1364015/annotation2392954/range2392954/1
type: Range
persistent identity: range2392954
display id: range2392954
version: 1
direction: direction/reverse
top level: BBa_K1364015
start: 1
end: 237
orientation: inline

YFP-N
type: ComponentDefinition
persistent identity: BBa K1616017
display id: BBa_K1616017
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: YN155 - N terminal YFP split
creator: Johanna Chesnel
created: 2015-09-17T11:00:00Z
modified: 2016-01-21T02:25:38Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2033_
m user id: 4206
owner id: 22805
owning group id: 9
status: status/Planning
bookmark: false
mutable description: A split protein is a protein whose sequence has been divided into two (or more) different parts. Often used to study protein-protein interactions, the protein can not perform its function until the parts are put back together. For instance, YFP, the yellow-fluorescent protein, will only express fluorescence when its two parts will be reunited. In normal condition, the production of a protein in response to a stimulus can easily reach several hours due to the many steps required for the protein synthesis. By using split-proteins, we are taking advantage of the absence of fluorescence when the two parts are apart. Indeed, the two parts of our split-YFP, when remaining separated, can be produced without being effective. Therefore, the overall process is far less time-consuming. However, to implement a light control on the fluorescence activation, a genetic construction gathering the VVD photoreceptor and our split-YFP has to be engineered. The new alternative approach for the visualization of protein interactiosn has been developed; the biomolecular fluorescence complementation (BiFC) techniques based on the complementation between fragments of fluorescent proteins; fragments of the yellow fluorescent protein (YFP) brought together by the association of two interaction partners fused to the fragments. They noticed that the spectral characteristics of BiFC of YFP were virtually identical to those of intact YFP.(Chang-Deng Hu, 2003)
mutable notes: Illegal sites have been checked.
mutable provenance: This part works with BBa_K16160016
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: YN155
sequence: https://synbiohub.org/public/igem/BBa_K1616017_sequence/1

YN155
type: SequenceAnnotation
persistent identity: annotation2470148
display id: annotation2470148
version: 1
top level: YFP-N
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1616017/annotation2470148/range2470148/1

https://synbiohub.org/public/igem/BBa_K1616017/annotation2470148/range2470148/1
type: Range
persistent identity: range2470148
display id: range2470148
version: 1
direction: direction/forward
top level: YFP-N
start: 1
end: 468
orientation: inline

Venus YFP
type: ComponentDefinition
persistent identity: BBa J63001
display id: BBa_J63001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: enhanced version of EYFP, yeast-optimized YFP
creator: Caroline Ajo-Franklin
created: 2006-10-10T11:00:00Z
modified: 2015-08-31T01:56:26Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _97_
m user id: 0
owner id: 545
owning group id: 97
sample status: In stock
status: status/Available
bookmark: false
mutable description: yeast codon-optimized fast folding variant of YFP
mutable notes: as published
mutable provenance: Thorn et al
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: Venus YFP
sequence: https://synbiohub.org/public/igem/BBa_J63001_sequence/1

Venus YFP
type: SequenceAnnotation
persistent identity: annotation1902850
display id: annotation1902850
version: 1
top level: Venus YFP
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J63001/annotation1902850/range1902850/1

https://synbiohub.org/public/igem/BBa_J63001/annotation1902850/range1902850/1
type: Range
persistent identity: range1902850
display id: range1902850
version: 1
direction: direction/forward
top level: Venus YFP
start: 1
end: 744
orientation: inline

BBa_K1072007
type: ComponentDefinition
persistent identity: BBa K1072007
display id: BBa_K1072007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Lux pI+RBS+LuxR+2TM+Lux pR+RBS+LuxI+2TM+Lux pR+RBS+GFP+2TM+Lux pR+RBS+α-ALS+2TM
creator: Junjie zhang, Yichong wang, Zewei yan, Rui chen and Xuenian chen
created: 2013-09-06T11:00:00Z
modified: 2015-05-08T01:09:01Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1381_
m user id: 0
owner id: 16002
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: BBa_K1072007 is a composite divece, including LuxR, LuxI, GFP and α-ALS genes. LuxR is under control of a constitutive promotor, Lux pI. Produce, LuxR, forms a complex with the quorum sensing molecule AHL and this complex subsequently increases the transcriptional rate of the lux pR promoter while also decreas the transcriptional rate of the lux pL promoter. The other genes are under control of this lux pR promoter. The generator, Lux pR+RBS+LuxI+B0015, express AHL which will combine with LuxR. GFP also is under control of Lux pR, presenting as reporter. α-ALS，as our desire produce, will transcrip activated by LuxR and AHL complex. If co-transforming with B0015 , this system will transcript α-ALS periodically. Finally, this part just is the combination of BBa_K1072000 and BBa_K1072006 .
mutable notes: -
mutable provenance: Some part get from registry and α-ALS gene originate from Lactococcus lactis, respectively.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1072000
component: BBa_K1072006
sequence annotation: BBa_K1072000
sequence annotation: BBa_K1072006
sequence: https://synbiohub.org/public/igem/BBa_K1072007_sequence/1

BBa_K1072000
type: Component
persistent identity: component2377998
display id: component2377998
version: 1
top level: BBa_K1072007
access: public
definition: BBa_K1072000

BBa_K1072006
type: Component
persistent identity: component2378014
display id: component2378014
version: 1
top level: BBa_K1072007
access: public
definition: https://synbiohub.org/public/igem/BBa_K1072006/1

BBa_K1072000
type: SequenceAnnotation
persistent identity: annotation2377998
display id: annotation2377998
version: 1
top level: BBa_K1072007
location: https://synbiohub.org/public/igem/BBa_K1072007/annotation2377998/range2377998/1
component: BBa_K1072000

https://synbiohub.org/public/igem/BBa_K1072007/annotation2377998/range2377998/1
type: Range
persistent identity: range2377998
display id: range2377998
version: 1
direction: direction/reverse
top level: BBa_K1072007
start: 1
end: 2910
orientation: inline

BBa_K1072006
type: SequenceAnnotation
persistent identity: annotation2378014
display id: annotation2378014
version: 1
top level: BBa_K1072007
location: https://synbiohub.org/public/igem/BBa_K1072007/annotation2378014/range2378014/1
component: BBa_K1072006

https://synbiohub.org/public/igem/BBa_K1072007/annotation2378014/range2378014/1
type: Range
persistent identity: range2378014
display id: range2378014
version: 1
direction: direction/reverse
top level: BBa_K1072007
start: 2919
end: 4801
orientation: inline

BBa_K1907007
type: ComponentDefinition
persistent identity: BBa K1907007
display id: BBa_K1907007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TSA1 promoter + Venus YFP
creator: Hele Haapaniemi
created: 2016-10-18T11:00:00Z
modified: 2016-10-19T02:12:34Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2373_
m user id: 29675
owner id: 29693
owning group id: 9
status: status/Planning
bookmark: false
mutable description: --
mutable notes: --
mutable provenance: --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1907004
component: BBa_K1907000
sequence annotation: BBa_K1907000
sequence annotation: BBa_K1907004
sequence: https://synbiohub.org/public/igem/BBa_K1907007_sequence/1

BBa_K1907004
type: Component
persistent identity: component2522324
display id: component2522324
version: 1
top level: BBa_K1907007
access: public
definition: https://synbiohub.org/public/igem/BBa_K1907004/1

BBa_K1907000
type: Component
persistent identity: component2522325
display id: component2522325
version: 1
top level: BBa_K1907007
access: public
definition: https://synbiohub.org/public/igem/BBa_K1907000/1

BBa_K1907000
type: SequenceAnnotation
persistent identity: annotation2522325
display id: annotation2522325
version: 1
top level: BBa_K1907007
location: https://synbiohub.org/public/igem/BBa_K1907007/annotation2522325/range2522325/1
component: BBa_K1907000

https://synbiohub.org/public/igem/BBa_K1907007/annotation2522325/range2522325/1
type: Range
persistent identity: range2522325
display id: range2522325
version: 1
direction: direction/reverse
top level: BBa_K1907007
start: 267
end: 983
orientation: inline

BBa_K1907004
type: SequenceAnnotation
persistent identity: annotation2522324
display id: annotation2522324
version: 1
top level: BBa_K1907007
location: https://synbiohub.org/public/igem/BBa_K1907007/annotation2522324/range2522324/1
component: BBa_K1907004

https://synbiohub.org/public/igem/BBa_K1907007/annotation2522324/range2522324/1
type: Range
persistent identity: range2522324
display id: range2522324
version: 1
direction: direction/reverse
top level: BBa_K1907007
start: 1
end: 260
orientation: inline

BBa_K294055
type: ComponentDefinition
persistent identity: BBa K294055
display id: BBa_K294055
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: GFP RFP Hybrid
creator: Ryoji Sekine
created: 2009-06-26T11:00:00Z
modified: 2016-01-25T02:34:10Z
discontinued: false
dominant: false
group u list: _397_
m user id: 4206
owner id: 5313
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This is a test. It may not bright.
mutable notes: E0040
mutable provenance: ATGCATATATATAATTT
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Hoge
sequence annotation: hogehoge
sequence: https://synbiohub.org/public/igem/BBa_K294055_sequence/1

Hoge
type: SequenceAnnotation
persistent identity: annotation2006753
display id: annotation2006753
version: 1
top level: BBa_K294055
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K294055/annotation2006753/range2006753/1

https://synbiohub.org/public/igem/BBa_K294055/annotation2006753/range2006753/1
type: Range
persistent identity: range2006753
display id: range2006753
version: 1
direction: direction/forward
top level: BBa_K294055
start: 200
end: 500
orientation: inline

hogehoge
type: SequenceAnnotation
persistent identity: annotation2006752
display id: annotation2006752
version: 1
top level: BBa_K294055
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K294055/annotation2006752/range2006752/1

https://synbiohub.org/public/igem/BBa_K294055/annotation2006752/range2006752/1
type: Range
persistent identity: range2006752
display id: range2006752
version: 1
direction: direction/forward
top level: BBa_K294055
start: 1
end: 100
orientation: inline

mRFP1
type: ComponentDefinition
persistent identity: BBa I13502
display id: BBa_I13502
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Screening plasmid intermediate
creator: jkm
created: 2005-05-30T11:00:00Z
modified: 2015-08-31T04:07:34Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_
m user id: 0
owner id: 253
owning group id: 6
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Built by Josh as an intermediate in screening plasmid construction.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_B0034
component: BBa_E1010
sequence annotation: BBa_B0034
sequence annotation: BBa_E1010
sequence: https://synbiohub.org/public/igem/BBa_I13502_sequence/1

BBa_B0034
type: Component
persistent identity: component1505038
display id: component1505038
version: 1
top level: mRFP1
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_E1010
type: Component
persistent identity: component1505045
display id: component1505045
version: 1
top level: mRFP1
access: public
definition: mRFP1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1505038
display id: annotation1505038
version: 1
top level: mRFP1
location: https://synbiohub.org/public/igem/BBa_I13502/annotation1505038/range1505038/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I13502/annotation1505038/range1505038/1
type: Range
persistent identity: range1505038
display id: range1505038
version: 1
direction: direction/forward
top level: mRFP1
start: 1
end: 12
orientation: inline

BBa_E1010
type: SequenceAnnotation
persistent identity: annotation1505045
display id: annotation1505045
version: 1
top level: mRFP1
location: https://synbiohub.org/public/igem/BBa_I13502/annotation1505045/range1505045/1
component: BBa_E1010

https://synbiohub.org/public/igem/BBa_I13502/annotation1505045/range1505045/1
type: Range
persistent identity: range1505045
display id: range1505045
version: 1
direction: direction/forward
top level: mRFP1
start: 19
end: 699
orientation: inline

BBa_K1033931
type: ComponentDefinition
persistent identity: BBa K1033931
display id: BBa_K1033931
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: amilGFP, yellow chromoprotein (incl RBS)
creator: Erik Lundin
created: 2013-09-16T11:00:00Z
modified: 2015-05-08T01:08:50Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1340_
m user id: 0
owner id: 13997
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This chromoprotein from the coral Acropora millepora, amilGFP, naturally exhibits strong yellow color when expressed. The color is readily visible to naked eye both in LB-culture and on agar plates. Color development can be seen in less than 24 hours of incubation.
mutable notes: Two illegal internal restriction sites (EcoRI and PstI) were removed.
mutable provenance: Acropora millepora. The DNA was provided by Jeffrey Miller at UCLA, and was made BioBrick-compatible by removing two illegal internal restriction sites (EcoRI and PstI).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_B0034
component: BBa_K592010
sequence annotation: BBa_B0034
sequence annotation: BBa_K592010
sequence: https://synbiohub.org/public/igem/BBa_K1033931_sequence/1

BBa_B0034
type: Component
persistent identity: component2351896
display id: component2351896
version: 1
top level: BBa_K1033931
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K592010
type: Component
persistent identity: component2351898
display id: component2351898
version: 1
top level: BBa_K1033931
access: public
definition: https://synbiohub.org/public/igem/BBa_K592010/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2351896
display id: annotation2351896
version: 1
top level: BBa_K1033931
location: https://synbiohub.org/public/igem/BBa_K1033931/annotation2351896/range2351896/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1033931/annotation2351896/range2351896/1
type: Range
persistent identity: range2351896
display id: range2351896
version: 1
direction: direction/reverse
top level: BBa_K1033931
start: 1
end: 12
orientation: inline

BBa_K592010
type: SequenceAnnotation
persistent identity: annotation2351898
display id: annotation2351898
version: 1
top level: BBa_K1033931
location: https://synbiohub.org/public/igem/BBa_K1033931/annotation2351898/range2351898/1
component: BBa_K592010

https://synbiohub.org/public/igem/BBa_K1033931/annotation2351898/range2351898/1
type: Range
persistent identity: range2351898
display id: range2351898
version: 1
direction: direction/reverse
top level: BBa_K1033931
start: 19
end: 717
orientation: inline

BBa_K993003
type: ComponentDefinition
persistent identity: BBa K993003
display id: BBa_K993003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Urease generator with ROSE thermometer
creator: FURKAN SACİT CEYLAN
created: 2013-06-13T11:00:00Z
modified: 2015-05-08T01:13:51Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _1290_
m user id: 0
owner id: 11155
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Repression of heat shock gene expression (ROSE) element The repression of heat shock gene expression (ROSE) element is an RNA element found in the 5' UTR of some heat shock protein's mRNAs. The ROSE element is an RNA thermometer that negatively regulates heat shock gene expression. The secondary structure is thought to be altered by temperature, thus it is an RNA thermometer. This structure blocks access to the ribosome binding site at normal temperatures. During heat shock however, the structure changes freeing the ribosome binding site and allowing expression to occur.[2][3] A partial structure of this RNA element has been determined using NMR.[1] ROSE1 and ROSEAT2 are specific examples of ROSE elements.[4] ROSE1 is found in Bradyrhizobium japonicum whereas ROSEAT2 is a closely related element from Agrobacterium tumefaciens. The two RNA elements have similar secondary structures with ROSE1 having an extra hairpin.[4] All ROSE elements contain a characteristic 'bulged G' opposite the Shine-Dalgarno sequence binding site, without this nucleotide the RNA thermometer loses its temperature-sensitivity.[5]
mutable notes: Note that RNA thermometer actually works as an RBS sequence. Therefore, this part does not include an additional ribosome binding site. Also, this part does not contain an terminator sequence, but a simple stop codon. The RFP should be translated continuously.
mutable provenance: AATTTTGTCAAAATAATTTTATTGACAACGTCTTATTAACGTTGATATAATTTAAATTTTTTTTGACAAAAATGGGCTCGTGTTGTACAATAAATGTGCCGCGACAAGCGGTCCGGGCGCCCTAGGGGCCCGGCGGAGACGGGCGCCGGAGGTGTCCGACGCCTGCTCGTCAAGTTCTTGCTCCTTGGAGGATATGAAGATAAACAGGCAGCAGTACGCGGAGAGCTACGGGCCGACGGTGGGGGACAGGGTGAGGCTGGCGGACACGGACCTGGGGGAGGTGGAGAAGGACTACTACTACCTGGGGGACGAGGTGAACTTCGGGGGGGGGAAGGTGCTGAGGGAGGGGATGGGGGAGAACGGGACGTACACGAGGACGGAGAACGTGTTGGACCTGCTGCTGACGAACGCGCTGATATTGGACTACACGGGGATATACAAGGCGGACATAGGGGTGAAGGACGGGTACATAGTGGGGATAGGGAAGGGGGGGAACCCGGACATAATGGACGGGGTGACGCCGAACATGATAGTGGGGACGGCGACGGAGGTGATAGCGGCGGAGGGGAAGATAGTGACGGCGGGGGGGATAGACACGCACGTGCACTTCATAAACCCGGACCAGGTGGACGTGGCGCTGGCGAACGGGATAACGACGCTGTTCGGGGGGGGGACGGGGCCGGCGGAGGGGAGCAAGGCGACGACGGTGACGCCGGGGCCGTGGAACATAGAGAAGATGCTGAAGAGCACGGAGGGGCTGCCGATAAACGTGGGGATACTGGGGAAGGGGCACGGGAGCAGCATAGCGCCGATAATGGAGCAGATAGACGCGGGGGCGGCGGGGCTGAAGATACACGAGGACTGGGGGGCGACGCCGGCGAGCATAGACAGGAGCCTGACGGTGGCGGACGAGGCGGACGTGCAGGTGGCGATACACAGCGACACGCTGAACGAGGCGGGGTTCTTGGAGGACACGGTGAGGGCGATAAACGGGAGGGTGATACACAGCTTCCACGTGGAGGGGGCGGGGGGGGGGCACGCGCCGGACATAATGGCGATGGCGGGGCACCCGAACGTGCTGCCGAGCAGCACGAACCCGACGAGGCCGTTCACGGTGAACACGATAGACGAGCACTTGGACATGCTGATGGTGTGCCACCACCTGAAGCAGAACATACCGGAGGACGTGGCGTTCGCGGACAGCAGGATAAGGCCGGAGACGATAGCGGCGGAGGACATACTGCACGACCTGGGGATAATAAGCATGATGAGCACGGACGCGCTGGCGATGGGGAGGGCGGGGGAGATGGTGCTGAGGACGTGGCAGACGGCGGACAAGATGAAGAAGCAGAGGGGGCCGCTGGCGGAGGAGAAGAACGGGAGCGACAACTTCAGGCTGAAGAGGTACGTGAGCAAGTACACGATAAACCCGGCGATAGCGCAGGGGATGGCGCACGAGGTGGGGAGCATAGAGGAGGGGAAGTTCGCGGACCTGGTGCTGTGGGAGCCGAAGTTCTTCGGGGTGAAGGCGGACAGGGTGATAAAGGGGGGGATAATAGCGTACGCGCAGATAGGGGACCCGAGCGCGAGCATACCGACGCCGCAGCCGGTGATGGGGAGGAGGATGTACGGGACGGTGGGGGACCTGATACACGACACGAACATAACGTTCATGAGCAAGAGCAGCATACAGCAGGGGGTGCCGGCGAAGCTGGGGCTGAAGAGGAGGATAGGGACGGTGAAGAATTGTCGAAACATAGGGAAGAAGGACATGAAGTGGAACGACGTGACGACGGACATAGACATAAACCCGGAGACGTACGAGGTGAAGGTGGACGGGGAGGTGCTGACGTGCGAGCCGGTGAAGGAGCTGCCGATGGCGCAGAGGTACTTCCTGTTCTAATCGCTGGGACGCCCGCCCCTAGAAAAGGAGGACTAAAAATGAGCAACAACAACTACATAGTGCCGGGGGAGTACAGGGTGGCGGAGGGGGAGATAGA…
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
sequence annotation: RBS
sequence annotation: Stop Codon
sequence annotation: Urease B
sequence annotation: ROSE Thermometer
sequence annotation: Insulator
sequence annotation: Start Codon
sequence annotation: Urease A
sequence annotation: Urease C
sequence annotation: pVeg2
sequence: https://synbiohub.org/public/igem/BBa_K993003_sequence/1

RBS
type: SequenceAnnotation
persistent identity: annotation2329461
display id: annotation2329461
version: 1
top level: BBa_K993003
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K993003/annotation2329461/range2329461/1

https://synbiohub.org/public/igem/BBa_K993003/annotation2329461/range2329461/1
type: Range
persistent identity: range2329461
display id: range2329461
version: 1
direction: direction/forward
top level: BBa_K993003
start: 1919
end: 1941
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2329465
display id: annotation2329465
version: 1
top level: BBa_K993003
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K993003/annotation2329465/range2329465/1

https://synbiohub.org/public/igem/BBa_K993003/annotation2329465/range2329465/1
type: Range
persistent identity: range2329465
display id: range2329465
version: 1
direction: direction/forward
top level: BBa_K993003
start: 2338
end: 2360
orientation: inline

Stop Codon
type: SequenceAnnotation
persistent identity: annotation2329471
display id: annotation2329471
version: 1
top level: BBa_K993003
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K993003/annotation2329471/range2329471/1

https://synbiohub.org/public/igem/BBa_K993003/annotation2329471/range2329471/1
type: Range
persistent identity: range2329471
display id: range2329471
version: 1
direction: direction/forward
top level: BBa_K993003
start: 2661
end: 2663
orientation: inline

Urease B
type: SequenceAnnotation
persistent identity: annotation2329463
display id: annotation2329463
version: 1
top level: BBa_K993003
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K993003/annotation2329463/range2329463/1

https://synbiohub.org/public/igem/BBa_K993003/annotation2329463/range2329463/1
type: Range
persistent identity: range2329463
display id: range2329463
version: 1
direction: direction/forward
top level: BBa_K993003
start: 1942
end: 2322
orientation: inline

ROSE Thermometer
type: SequenceAnnotation
persistent identity: annotation2329458
display id: annotation2329458
version: 1
top level: BBa_K993003
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K993003/annotation2329458/range2329458/1

https://synbiohub.org/public/igem/BBa_K993003/annotation2329458/range2329458/1
type: Range
persistent identity: range2329458
display id: range2329458
version: 1
direction: direction/forward
top level: BBa_K993003
start: 98
end: 193
orientation: inline

Insulator
type: SequenceAnnotation
persistent identity: annotation2329460
display id: annotation2329460
version: 1
top level: BBa_K993003
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K993003/annotation2329460/range2329460/1

https://synbiohub.org/public/igem/BBa_K993003/annotation2329460/range2329460/1
type: Range
persistent identity: range2329460
display id: range2329460
version: 1
direction: direction/forward
top level: BBa_K993003
start: 1904
end: 1918
orientation: inline

Stop Codon
type: SequenceAnnotation
persistent identity: annotation2329469
display id: annotation2329469
version: 1
top level: BBa_K993003
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K993003/annotation2329469/range2329469/1

https://synbiohub.org/public/igem/BBa_K993003/annotation2329469/range2329469/1
type: Range
persistent identity: range2329469
display id: range2329469
version: 1
direction: direction/forward
top level: BBa_K993003
start: 1901
end: 1903
orientation: inline

Insulator
type: SequenceAnnotation
persistent identity: annotation2329464
display id: annotation2329464
version: 1
top level: BBa_K993003
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K993003/annotation2329464/range2329464/1

https://synbiohub.org/public/igem/BBa_K993003/annotation2329464/range2329464/1
type: Range
persistent identity: range2329464
display id: range2329464
version: 1
direction: direction/forward
top level: BBa_K993003
start: 2323
end: 2337
orientation: inline

Start Codon
type: SequenceAnnotation
persistent identity: annotation2329468
display id: annotation2329468
version: 1
top level: BBa_K993003
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K993003/annotation2329468/range2329468/1

https://synbiohub.org/public/igem/BBa_K993003/annotation2329468/range2329468/1
type: Range
persistent identity: range2329468
display id: range2329468
version: 1
direction: direction/forward
top level: BBa_K993003
start: 2361
end: 2363
orientation: inline

Start Codon
type: SequenceAnnotation
persistent identity: annotation2329462
display id: annotation2329462
version: 1
top level: BBa_K993003
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K993003/annotation2329462/range2329462/1

https://synbiohub.org/public/igem/BBa_K993003/annotation2329462/range2329462/1
type: Range
persistent identity: range2329462
display id: range2329462
version: 1
direction: direction/forward
top level: BBa_K993003
start: 194
end: 196
orientation: inline

Urease A
type: SequenceAnnotation
persistent identity: annotation2329459
display id: annotation2329459
version: 1
top level: BBa_K993003
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K993003/annotation2329459/range2329459/1

https://synbiohub.org/public/igem/BBa_K993003/annotation2329459/range2329459/1
type: Range
persistent identity: range2329459
display id: range2329459
version: 1
direction: direction/forward
top level: BBa_K993003
start: 194
end: 1903
orientation: inline

Urease C
type: SequenceAnnotation
persistent identity: annotation2329466
display id: annotation2329466
version: 1
top level: BBa_K993003
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K993003/annotation2329466/range2329466/1

https://synbiohub.org/public/igem/BBa_K993003/annotation2329466/range2329466/1
type: Range
persistent identity: range2329466
display id: range2329466
version: 1
direction: direction/forward
top level: BBa_K993003
start: 2361
end: 2663
orientation: inline

Start Codon
type: SequenceAnnotation
persistent identity: annotation2329467
display id: annotation2329467
version: 1
top level: BBa_K993003
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K993003/annotation2329467/range2329467/1

https://synbiohub.org/public/igem/BBa_K993003/annotation2329467/range2329467/1
type: Range
persistent identity: range2329467
display id: range2329467
version: 1
direction: direction/forward
top level: BBa_K993003
start: 1942
end: 1944
orientation: inline

Stop Codon
type: SequenceAnnotation
persistent identity: annotation2329470
display id: annotation2329470
version: 1
top level: BBa_K993003
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K993003/annotation2329470/range2329470/1

https://synbiohub.org/public/igem/BBa_K993003/annotation2329470/range2329470/1
type: Range
persistent identity: range2329470
display id: range2329470
version: 1
direction: direction/forward
top level: BBa_K993003
start: 2320
end: 2322
orientation: inline

pVeg2
type: SequenceAnnotation
persistent identity: annotation2329447
display id: annotation2329447
version: 1
top level: BBa_K993003
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K993003/annotation2329447/range2329447/1

https://synbiohub.org/public/igem/BBa_K993003/annotation2329447/range2329447/1
type: Range
persistent identity: range2329447
display id: range2329447
version: 1
direction: direction/forward
top level: BBa_K993003
start: 1
end: 97
orientation: inline

dTomato
type: ComponentDefinition
persistent identity: BBa K1688010
display id: BBa_K1688010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: dTomato (inc RBS, J23101 promoter)
creator: Dianna Zeleskov
created: 2015-09-14T11:00:00Z
modified: 2015-09-18T11:41:03Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2106_
m user id: 27796
owner id: 27121
owning group id: 9
status: status/Planning
bookmark: false
mutable description: The dTomato protein is a fluorescent dimer protein that emits orange-red light when it is excited by green-yellow light. It is preferable to use ??? especially in self-made fluorometry tests ??? because the excitation wavelengths and the emission wavelengths do not overlap as much as in other fluorescent proteins. The dTomato excitation peak is at 554 nm and 50% of it is at 510 nm. The emission peak is at 581 nm and the 50% emission peak is at 629 nm. Characterization has been made with the Anderson promoters BBa_J23101 (this construct) and BBa_J23110 (construct BBa_K1688011)
mutable provenance: dTomato was derived from a monomeric mutant of the anthozoan coral RFP, DsRed.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_K1688004
component: BBa_J23101
sequence annotation: BBa_J23101
sequence annotation: BBa_K1688004
sequence: https://synbiohub.org/public/igem/BBa_K1688010_sequence/1

BBa_K1688004
type: Component
persistent identity: component2457522
display id: component2457522
version: 1
top level: dTomato
access: public
definition: dTomato

BBa_J23101
type: Component
persistent identity: component2457518
display id: component2457518
version: 1
top level: dTomato
access: public
definition: https://synbiohub.org/public/igem/BBa_J23101/1

BBa_J23101
type: SequenceAnnotation
persistent identity: annotation2457518
display id: annotation2457518
version: 1
top level: dTomato
location: https://synbiohub.org/public/igem/BBa_K1688010/annotation2457518/range2457518/1
component: BBa_J23101

https://synbiohub.org/public/igem/BBa_K1688010/annotation2457518/range2457518/1
type: Range
persistent identity: range2457518
display id: range2457518
version: 1
direction: direction/reverse
top level: dTomato
start: 1
end: 35
orientation: inline

BBa_K1688004
type: SequenceAnnotation
persistent identity: annotation2457522
display id: annotation2457522
version: 1
top level: dTomato
location: https://synbiohub.org/public/igem/BBa_K1688010/annotation2457522/range2457522/1
component: BBa_K1688004

https://synbiohub.org/public/igem/BBa_K1688010/annotation2457522/range2457522/1
type: Range
persistent identity: range2457522
display id: range2457522
version: 1
direction: direction/reverse
top level: dTomato
start: 44
end: 769
orientation: inline

BBa_K389016
type: ComponentDefinition
persistent identity: BBa K389016
display id: BBa_K389016
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: VirA/G reporter device mRFP
creator: Jonas Aretz
created: 2010-09-27T11:00:00Z
modified: 2015-05-08T01:12:19Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _517_
m user id: 0
owner id: 6670
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This BioBrick is for testing the virA/G signaling system. The read-out is the mRFP E1010 . This system contains an unmutated ''virA'', so it is possible to measure, how the natural virA/G system works and reacts.
mutable notes: this is only for measuring the natural virA/G system
mutable provenance: ''A. tumefaciens'' C58 TI-plasmid, partsregistry, Mr. Gene
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K389010
component: BBa_K389013
sequence annotation: BBa_K389010
sequence annotation: BBa_K389013
sequence: https://synbiohub.org/public/igem/BBa_K389016_sequence/1

BBa_K389010
type: Component
persistent identity: component2277648
display id: component2277648
version: 1
top level: BBa_K389016
access: public
definition: https://synbiohub.org/public/igem/BBa_K389010/1

BBa_K389013
type: Component
persistent identity: component2277667
display id: component2277667
version: 1
top level: BBa_K389016
access: public
definition: BBa_K389013

BBa_K389010
type: SequenceAnnotation
persistent identity: annotation2277648
display id: annotation2277648
version: 1
top level: BBa_K389016
location: https://synbiohub.org/public/igem/BBa_K389016/annotation2277648/range2277648/1
component: BBa_K389010

https://synbiohub.org/public/igem/BBa_K389016/annotation2277648/range2277648/1
type: Range
persistent identity: range2277648
display id: range2277648
version: 1
direction: direction/reverse
top level: BBa_K389016
start: 1
end: 2566
orientation: inline

BBa_K389013
type: SequenceAnnotation
persistent identity: annotation2277667
display id: annotation2277667
version: 1
top level: BBa_K389016
location: https://synbiohub.org/public/igem/BBa_K389016/annotation2277667/range2277667/1
component: BBa_K389013

https://synbiohub.org/public/igem/BBa_K389016/annotation2277667/range2277667/1
type: Range
persistent identity: range2277667
display id: range2277667
version: 1
direction: direction/reverse
top level: BBa_K389016
start: 2575
end: 4411
orientation: inline

SBFP2
type: ComponentDefinition
persistent identity: BBa K156010
display id: BBa_K156010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: SBFP2 (strongly enhanced blue fluorescent protein)
creator: George McArthur IV
created: 2008-10-28T12:00:00Z
modified: 2016-01-26T09:51:52Z
discontinued: false
dominant: false
experience: experience/None
group u list: _246_
m user id: 4206
owner id: 1475
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This part codes for orange fluorescent protein (OFP). Fluorescent proteins are useful tools for visualizing and quantifying protein expression in cells. OFP is yet another fluorescent protein, like GFP, that can be utilized as a reporter.
mutable notes: This part was codon-optimized for expression in ''E. coli''. The following restriction enzyme sites were also removed: EcoRI, NotI, XbaI, SpeI, PstI, NheI, AvrII, ApoI, MfeI, NsiI, SbfI.
mutable provenance: OFP was cloned from the tentacles of ''Cerianthus'' sp., a tube anemone. The original sequence was obtained from GenBank (VERSION AY296063.1, GI:31616578).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K156010_sequence/1

GFP
type: ComponentDefinition
persistent identity: BBa K845002
display id: BBa_K845002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Strong RBS GFPmut2
creator: Gr??gory Hansen
created: 2012-09-25T11:00:00Z
modified: 2015-05-08T01:13:33Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1105_
m user id: 0
owner id: 12465
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: RBS shine d'algarno GFP mut2
mutable notes: http://www.weizmann.ac.il/mcb/UriAlon/Papers/Zaslaver_Ecoli_library.pdf
mutable provenance: The protein comes from Aequorea victoria. This jellyfish uses GFP (Green Fluorescent Protein) in order to convert the blue luminescence emitted by the aequorine into a green luminescence
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: Strong RBS
sequence annotation: GFPmut2
sequence: https://synbiohub.org/public/igem/BBa_K845002_sequence/1

Strong RBS
type: SequenceAnnotation
persistent identity: annotation2203924
display id: annotation2203924
version: 1
top level: GFP
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K845002/annotation2203924/range2203924/1

https://synbiohub.org/public/igem/BBa_K845002/annotation2203924/range2203924/1
type: Range
persistent identity: range2203924
display id: range2203924
version: 1
direction: direction/forward
top level: GFP
start: 1
end: 26
orientation: inline

GFPmut2
type: SequenceAnnotation
persistent identity: annotation2203925
display id: annotation2203925
version: 1
top level: GFP
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K845002/annotation2203925/range2203925/1

https://synbiohub.org/public/igem/BBa_K845002/annotation2203925/range2203925/1
type: Range
persistent identity: range2203925
display id: range2203925
version: 1
direction: direction/forward
top level: GFP
start: 26
end: 743
orientation: inline

BBa_K1740000
type: ComponentDefinition
persistent identity: BBa K1740000
display id: BBa_K1740000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Dronpa 145N ORF (E. coli optimized)
creator: Yukina Tanimura,Keisuke Sugahara,
created: 2015-08-19T11:00:00Z
modified: 2016-01-25T11:17:08Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2162_
m user id: 4206
owner id: 25441
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This is test
mutable notes: Non
mutable provenance: synthesis DNA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: strat codon
sequence annotation: Dronpa 145N
sequence annotation: 145Lys to 145Asn
sequence: https://synbiohub.org/public/igem/BBa_K1740000_sequence/1

strat codon
type: SequenceAnnotation
persistent identity: annotation2444415
display id: annotation2444415
version: 1
top level: BBa_K1740000
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1740000/annotation2444415/range2444415/1

https://synbiohub.org/public/igem/BBa_K1740000/annotation2444415/range2444415/1
type: Range
persistent identity: range2444415
display id: range2444415
version: 1
direction: direction/forward
top level: BBa_K1740000
start: 1
end: 3
orientation: inline

Dronpa 145N
type: SequenceAnnotation
persistent identity: annotation2444414
display id: annotation2444414
version: 1
top level: BBa_K1740000
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1740000/annotation2444414/range2444414/1

https://synbiohub.org/public/igem/BBa_K1740000/annotation2444414/range2444414/1
type: Range
persistent identity: range2444414
display id: range2444414
version: 1
direction: direction/forward
top level: BBa_K1740000
start: 1
end: 672
orientation: inline

145Lys to 145Asn
type: SequenceAnnotation
persistent identity: annotation2444413
display id: annotation2444413
version: 1
top level: BBa_K1740000
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K1740000/annotation2444413/range2444413/1

https://synbiohub.org/public/igem/BBa_K1740000/annotation2444413/range2444413/1
type: Range
persistent identity: range2444413
display id: range2444413
version: 1
direction: direction/forward
top level: BBa_K1740000
start: 433
end: 435
orientation: inline

SgsE | RFP
type: ComponentDefinition
persistent identity: BBa K525304
display id: BBa_K525304
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Fusion Protein of S-Layer SgsE and mCherry RFP
creator: Timo Wolf
created: 2011-09-09T11:00:00Z
modified: 2016-01-26T10:06:30Z
discontinued: false
dominant: false
experience: experience/None
group u list: _690_
m user id: 4206
owner id: 6668
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Fusion Protein of S-Layer SgsE and mCherry RFP
mutable notes: Fusion Protein of BBa_K525303 and BBa_J18932
mutable provenance: Fusion Protein of S-Layer SgsE and mCherry RFP
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: RBS
sequence annotation: B0034
sequence annotation: Freiburg Scar
sequence annotation: T7 promoter
sequence annotation: Age I
sequence annotation: mCherry (RFP)
sequence annotation: lac operator
sequence annotation: ATG
sequence annotation: K525301
sequence annotation: NgoMIV
sequence annotation: SgsE domain
sequence annotation: J18932
sequence annotation: TAA
sequence: https://synbiohub.org/public/igem/BBa_K525304_sequence/1

RBS
type: SequenceAnnotation
persistent identity: annotation2128569
display id: annotation2128569
version: 1
top level: SgsE | RFP
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K525304/annotation2128569/range2128569/1

https://synbiohub.org/public/igem/BBa_K525304/annotation2128569/range2128569/1
type: Range
persistent identity: range2128569
display id: range2128569
version: 1
direction: direction/forward
top level: SgsE | RFP
start: 56
end: 59
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation2128562
display id: annotation2128562
version: 1
top level: SgsE | RFP
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K525304/annotation2128562/range2128562/1

https://synbiohub.org/public/igem/BBa_K525304/annotation2128562/range2128562/1
type: Range
persistent identity: range2128562
display id: range2128562
version: 1
direction: direction/forward
top level: SgsE | RFP
start: 53
end: 64
orientation: inline

Freiburg Scar
type: SequenceAnnotation
persistent identity: annotation2128586
display id: annotation2128586
version: 1
top level: SgsE | RFP
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K525304/annotation2128586/range2128586/1

https://synbiohub.org/public/igem/BBa_K525304/annotation2128586/range2128586/1
type: Range
persistent identity: range2128586
display id: range2128586
version: 1
direction: direction/forward
top level: SgsE | RFP
start: 2401
end: 2406
orientation: inline

T7 promoter
type: SequenceAnnotation
persistent identity: annotation2128563
display id: annotation2128563
version: 1
top level: SgsE | RFP
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K525304/annotation2128563/range2128563/1

https://synbiohub.org/public/igem/BBa_K525304/annotation2128563/range2128563/1
type: Range
persistent identity: range2128563
display id: range2128563
version: 1
direction: direction/forward
top level: SgsE | RFP
start: 1
end: 20
orientation: inline

Age I
type: SequenceAnnotation
persistent identity: annotation2128571
display id: annotation2128571
version: 1
top level: SgsE | RFP
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K525304/annotation2128571/range2128571/1

https://synbiohub.org/public/igem/BBa_K525304/annotation2128571/range2128571/1
type: Range
persistent identity: range2128571
display id: range2128571
version: 1
direction: direction/forward
top level: SgsE | RFP
start: 3112
end: 3117
orientation: inline

mCherry (RFP)
type: SequenceAnnotation
persistent identity: annotation2128565
display id: annotation2128565
version: 1
top level: SgsE | RFP
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K525304/annotation2128565/range2128565/1

https://synbiohub.org/public/igem/BBa_K525304/annotation2128565/range2128565/1
type: Range
persistent identity: range2128565
display id: range2128565
version: 1
direction: direction/forward
top level: SgsE | RFP
start: 2407
end: 3120
orientation: inline

lac operator
type: SequenceAnnotation
persistent identity: annotation2128568
display id: annotation2128568
version: 1
top level: SgsE | RFP
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K525304/annotation2128568/range2128568/1

https://synbiohub.org/public/igem/BBa_K525304/annotation2128568/range2128568/1
type: Range
persistent identity: range2128568
display id: range2128568
version: 1
direction: direction/forward
top level: SgsE | RFP
start: 20
end: 44
orientation: inline

ATG
type: SequenceAnnotation
persistent identity: annotation2128570
display id: annotation2128570
version: 1
top level: SgsE | RFP
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K525304/annotation2128570/range2128570/1

https://synbiohub.org/public/igem/BBa_K525304/annotation2128570/range2128570/1
type: Range
persistent identity: range2128570
display id: range2128570
version: 1
direction: direction/forward
top level: SgsE | RFP
start: 71
end: 73
orientation: inline

K525301
type: SequenceAnnotation
persistent identity: annotation2128564
display id: annotation2128564
version: 1
top level: SgsE | RFP
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K525304/annotation2128564/range2128564/1

https://synbiohub.org/public/igem/BBa_K525304/annotation2128564/range2128564/1
type: Range
persistent identity: range2128564
display id: range2128564
version: 1
direction: direction/forward
top level: SgsE | RFP
start: 71
end: 2400
orientation: inline

NgoMIV
type: SequenceAnnotation
persistent identity: annotation2128572
display id: annotation2128572
version: 1
top level: SgsE | RFP
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K525304/annotation2128572/range2128572/1

https://synbiohub.org/public/igem/BBa_K525304/annotation2128572/range2128572/1
type: Range
persistent identity: range2128572
display id: range2128572
version: 1
direction: direction/forward
top level: SgsE | RFP
start: 76
end: 81
orientation: inline

SgsE domain
type: SequenceAnnotation
persistent identity: annotation2128566
display id: annotation2128566
version: 1
top level: SgsE | RFP
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K525304/annotation2128566/range2128566/1

https://synbiohub.org/public/igem/BBa_K525304/annotation2128566/range2128566/1
type: Range
persistent identity: range2128566
display id: range2128566
version: 1
direction: direction/forward
top level: SgsE | RFP
start: 71
end: 2400
orientation: inline

J18932
type: SequenceAnnotation
persistent identity: annotation2128561
display id: annotation2128561
version: 1
top level: SgsE | RFP
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K525304/annotation2128561/range2128561/1

https://synbiohub.org/public/igem/BBa_K525304/annotation2128561/range2128561/1
type: Range
persistent identity: range2128561
display id: range2128561
version: 1
direction: direction/forward
top level: SgsE | RFP
start: 2407
end: 3120
orientation: inline

TAA
type: SequenceAnnotation
persistent identity: annotation2128567
display id: annotation2128567
version: 1
top level: SgsE | RFP
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K525304/annotation2128567/range2128567/1

https://synbiohub.org/public/igem/BBa_K525304/annotation2128567/range2128567/1
type: Range
persistent identity: range2128567
display id: range2128567
version: 1
direction: direction/forward
top level: SgsE | RFP
start: 3118
end: 3120
orientation: inline

mCherry
type: ComponentDefinition
persistent identity: BBa K105010
display id: BBa_K105010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: mCherry, Yeast optimized for fusion proteins
creator: Marius M??ller, Paul Bourgine
created: 2008-10-11T11:00:00Z
modified: 2015-05-08T01:08:52Z
discontinued: false
dominant: false
experience: experience/None
group u list: _253_
m user id: 0
owner id: 3313
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Our BioBrick is red fluorescent protein which can be used to label proteins in yeast. Therefor it has specific characteristics of a BioBrick part for fusion proteins (see design details). <br\n>
mutable notes: To enable the fusion with other domains in frame the vector of this BioBrick has no base pair in between the restriction side and the BioBrick. Furthermore, this coding sequence does not include a start codon.<br\n> For more information about this issus, see:<br\n> Phillips, I.E. and Silver, P.A. "A new Biobrick Assembly Strategy Designed for Facile Protein Engineering." <br\n> DSpace http://hdl.handle.net/1721.1/32535 (2006).
mutable provenance: We changed the format of BBa_E2060 into Phillips/Silver standard.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence: https://synbiohub.org/public/igem/BBa_K105010_sequence/1

BBa_K1031422
type: ComponentDefinition
persistent identity: BBa K1031422
display id: BBa_K1031422
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ph(HcaR)-B0034-sfGFP
creator: He Shuaixin
created: 2013-10-17T11:00:00Z
modified: 2015-05-08T01:08:47Z
discontinued: false
dominant: false
group u list: _1338_
m user id: 0
owner id: 15843
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Ph(HcaR)-B0034-sfGFP
mutable notes: molecular cloning
mutable provenance: E.coli
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: B0034
sequence annotation: sfGFP
sequence annotation: B0015
sequence annotation: Ph (HcaR)
sequence: https://synbiohub.org/public/igem/BBa_K1031422_sequence/1

B0034
type: SequenceAnnotation
persistent identity: annotation2369615
display id: annotation2369615
version: 1
top level: BBa_K1031422
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1031422/annotation2369615/range2369615/1

https://synbiohub.org/public/igem/BBa_K1031422/annotation2369615/range2369615/1
type: Range
persistent identity: range2369615
display id: range2369615
version: 1
direction: direction/forward
top level: BBa_K1031422
start: 84
end: 95
orientation: inline

sfGFP
type: SequenceAnnotation
persistent identity: annotation2369614
display id: annotation2369614
version: 1
top level: BBa_K1031422
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1031422/annotation2369614/range2369614/1

https://synbiohub.org/public/igem/BBa_K1031422/annotation2369614/range2369614/1
type: Range
persistent identity: range2369614
display id: range2369614
version: 1
direction: direction/forward
top level: BBa_K1031422
start: 101
end: 823
orientation: inline

B0015
type: SequenceAnnotation
persistent identity: annotation2369616
display id: annotation2369616
version: 1
top level: BBa_K1031422
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1031422/annotation2369616/range2369616/1

https://synbiohub.org/public/igem/BBa_K1031422/annotation2369616/range2369616/1
type: Range
persistent identity: range2369616
display id: range2369616
version: 1
direction: direction/forward
top level: BBa_K1031422
start: 830
end: 958
orientation: inline

Ph (HcaR)
type: SequenceAnnotation
persistent identity: annotation2369613
display id: annotation2369613
version: 1
top level: BBa_K1031422
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1031422/annotation2369613/range2369613/1

https://synbiohub.org/public/igem/BBa_K1031422/annotation2369613/range2369613/1
type: Range
persistent identity: range2369613
display id: range2369613
version: 1
direction: direction/forward
top level: BBa_K1031422
start: 1
end: 74
orientation: inline

BBa_K1399020
type: ComponentDefinition
persistent identity: BBa K1399020
display id: BBa_K1399020
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ptet-GFP(LVA)
creator: Anna Stikane
created: 2014-09-18T11:00:00Z
modified: 2015-05-08T01:10:16Z
discontinued: false
dominant: false
group u list: _1777_
m user id: 0
owner id: 22477
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Tetracycline/aTc inducible promoter with GFP reporter tagged with LVA-ssrA degradation tag followed by terminator. The tagged GFP is actively degraded within cell, thus provides better temporal resolution of green fluorescence and promoter activity.
mutable notes: Part was assembled using BrickClip assembly (BBF RFC104) using other biobrick parts as templates. BrickClip assembly is a special case of more general Paperclip assembly method.[1]
mutable provenance: other biobricks
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_R0040
component: BBa_B0015
component: BBa_K1399004
component: BBa_B0034
sequence annotation: BBa_K1399004
sequence annotation: BBa_R0040
sequence annotation: BBa_B0015
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K1399020_sequence/1

BBa_R0040
type: Component
persistent identity: component2384239
display id: component2384239
version: 1
top level: BBa_K1399020
access: public
definition: https://synbiohub.org/public/igem/BBa_R0040/1

BBa_B0015
type: Component
persistent identity: component2384258
display id: component2384258
version: 1
top level: BBa_K1399020
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_K1399004
type: Component
persistent identity: component2384251
display id: component2384251
version: 1
top level: BBa_K1399020
access: public
definition: BBa_K1399004

BBa_B0034
type: Component
persistent identity: component2384245
display id: component2384245
version: 1
top level: BBa_K1399020
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K1399004
type: SequenceAnnotation
persistent identity: annotation2384251
display id: annotation2384251
version: 1
top level: BBa_K1399020
location: https://synbiohub.org/public/igem/BBa_K1399020/annotation2384251/range2384251/1
component: BBa_K1399004

https://synbiohub.org/public/igem/BBa_K1399020/annotation2384251/range2384251/1
type: Range
persistent identity: range2384251
display id: range2384251
version: 1
direction: direction/reverse
top level: BBa_K1399020
start: 81
end: 833
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2384239
display id: annotation2384239
version: 1
top level: BBa_K1399020
location: https://synbiohub.org/public/igem/BBa_K1399020/annotation2384239/range2384239/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_K1399020/annotation2384239/range2384239/1
type: Range
persistent identity: range2384239
display id: range2384239
version: 1
direction: direction/forward
top level: BBa_K1399020
start: 1
end: 54
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2384258
display id: annotation2384258
version: 1
top level: BBa_K1399020
location: https://synbiohub.org/public/igem/BBa_K1399020/annotation2384258/range2384258/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K1399020/annotation2384258/range2384258/1
type: Range
persistent identity: range2384258
display id: range2384258
version: 1
direction: direction/reverse
top level: BBa_K1399020
start: 842
end: 970
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2384245
display id: annotation2384245
version: 1
top level: BBa_K1399020
location: https://synbiohub.org/public/igem/BBa_K1399020/annotation2384245/range2384245/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1399020/annotation2384245/range2384245/1
type: Range
persistent identity: range2384245
display id: range2384245
version: 1
direction: direction/reverse
top level: BBa_K1399020
start: 63
end: 74
orientation: inline

mCherry
type: ComponentDefinition
persistent identity: BBa J06505
display id: BBa_J06505
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: monomeric RFP optimized for bacteria
creator: ytwang
created: 2005-07-17T11:00:00Z
modified: 2015-08-31T04:08:18Z
discontinued: false
dominant: false
experience: experience/None
group u list: _20_
m user id: 0
owner id: 340
owning group id: 20
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: mRFP (DsRed) derived, altered to be a biobrick by removing a PstI site and adding in the ends.
mutable notes: Made a point mutation to eliminate a PstI site in the middle and then added BioBrick ends with the LVA tag using PCR. Sequenced using primers that bind to the pSB1A2 plasmid on either side of the brick.
mutable provenance: mCherry from Roger Y. Tsien's lab, altered to be BioBrick compatible and with an LVA tag added.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: LVA
sequence annotation: mCherry
sequence annotation: C->T (removing PstI site)
sequence: https://synbiohub.org/public/igem/BBa_J06505_sequence/1

LVA
type: SequenceAnnotation
persistent identity: annotation1585887
display id: annotation1585887
version: 1
top level: mCherry
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_J06505/annotation1585887/range1585887/1

https://synbiohub.org/public/igem/BBa_J06505/annotation1585887/range1585887/1
type: Range
persistent identity: range1585887
display id: range1585887
version: 1
direction: direction/forward
top level: mCherry
start: 709
end: 717
orientation: inline

mCherry
type: SequenceAnnotation
persistent identity: annotation1585879
display id: annotation1585879
version: 1
top level: mCherry
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J06505/annotation1585879/range1585879/1

https://synbiohub.org/public/igem/BBa_J06505/annotation1585879/range1585879/1
type: Range
persistent identity: range1585879
display id: range1585879
version: 1
direction: direction/forward
top level: mCherry
start: 1
end: 708
orientation: inline

C->T (removing PstI site)
type: SequenceAnnotation
persistent identity: annotation1585880
display id: annotation1585880
version: 1
top level: mCherry
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_J06505/annotation1585880/range1585880/1

https://synbiohub.org/public/igem/BBa_J06505/annotation1585880/range1585880/1
type: Range
persistent identity: range1585880
display id: range1585880
version: 1
direction: direction/forward
top level: mCherry
start: 352
end: 352
orientation: inline

BBa_K819006
type: ComponentDefinition
persistent identity: BBa K819006
display id: BBa_K819006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Testing device for Luminesensor
creator: YU Zhou
created: 2012-09-01T11:00:00Z
modified: 2015-05-08T01:13:29Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1077_
m user id: 0
owner id: 13785
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: a fast degrading GFP ligated to the rear of a sulA promoter in 408 form (only recognizable by our LexA-VVD fusing protein, not by E.coli endogenous LexA). The sulA promoter promotes a gene which express SulA protein, a differentiation inhibitor, and was a member of the SOS regulon family. When co-transformed with our luminesensor plasmid into E.coli cell illuminated by blue light, the light will triger the dimerizaiton of the LexA408-VVD fusion protein and the dimerized LexA408 domain will bind the SOS box in 408 form in the SulA408 promoter to inhibit the transcription of the downstream gene so that no GFP will be expressed; if the environment is dark, the luminesensor will not dimerize and no supression of the promoter will occour, and GFP will be expressed.
mutable notes: GFP ligated to the rear of a sulA promoter in 408 form (only recognizable by our LexA-VVD fusing protein, not by E.coli endogenous LexA).
mutable provenance: GFP with ssrA tag was from fromer Peking iGEM team members.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: RBS-GFP-ssrA:BBa_K581007
sequence annotation: SulA408 Promoter
sequence: https://synbiohub.org/public/igem/BBa_K819006_sequence/1

RBS-GFP-ssrA:BBa_K581007
type: SequenceAnnotation
persistent identity: annotation2197257
display id: annotation2197257
version: 1
top level: BBa_K819006
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K819006/annotation2197257/range2197257/1

https://synbiohub.org/public/igem/BBa_K819006/annotation2197257/range2197257/1
type: Range
persistent identity: range2197257
display id: range2197257
version: 1
direction: direction/forward
top level: BBa_K819006
start: 70
end: 846
orientation: inline

SulA408 Promoter
type: SequenceAnnotation
persistent identity: annotation2197256
display id: annotation2197256
version: 1
top level: BBa_K819006
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K819006/annotation2197256/range2197256/1

https://synbiohub.org/public/igem/BBa_K819006/annotation2197256/range2197256/1
type: Range
persistent identity: range2197256
display id: range2197256
version: 1
direction: direction/forward
top level: BBa_K819006
start: 1
end: 66
orientation: inline

BBa_K125500
type: ComponentDefinition
persistent identity: BBa K125500
display id: BBa_K125500
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: GFP fusion brick
creator: Grace Kwan
created: 2008-07-24T11:00:00Z
modified: 2016-01-25T02:34:56Z
discontinued: false
dominant: false
experience: experience/None
group u list: _179_
m user id: 4206
owner id: 3229
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: The first two nucleotides of the GFP start codon were removed to create this fusion brick.
mutable provenance: The GFP fusion brick was derived from the GFP BioBrick, [http://partsregistry.org/Part:BBa_E0040 BBa_E0040].
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: GFP fusion brick
sequence annotation: deletion of TG
sequence: https://synbiohub.org/public/igem/BBa_K125500_sequence/1

GFP fusion brick
type: SequenceAnnotation
persistent identity: annotation1968260
display id: annotation1968260
version: 1
top level: BBa_K125500
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K125500/annotation1968260/range1968260/1

https://synbiohub.org/public/igem/BBa_K125500/annotation1968260/range1968260/1
type: Range
persistent identity: range1968260
display id: range1968260
version: 1
direction: direction/forward
top level: BBa_K125500
start: 1
end: 718
orientation: inline

deletion of TG
type: SequenceAnnotation
persistent identity: annotation1969908
display id: annotation1969908
version: 1
top level: BBa_K125500
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_K125500/annotation1969908/range1969908/1

https://synbiohub.org/public/igem/BBa_K125500/annotation1969908/range1969908/1
type: Range
persistent identity: range1969908
display id: range1969908
version: 1
direction: direction/forward
top level: BBa_K125500
start: 1
end: 1
orientation: inline

BBa_K1980010
type: ComponentDefinition
persistent identity: BBa K1980010
display id: BBa_K1980010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pCopA TAT Csp1 sfGFP with divergent CueR
creator: Sam Garforth
created: 2016-10-10T11:00:00Z
modified: 2016-10-24T08:12:51Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _2447_
m user id: 29607
owner id: 29607
owning group id: 9
status: status/Planning
bookmark: false
mutable description: pCopA TAT Csp1 sfGFP with plasmid constitutively expressed CueR
mutable notes: aahahahah
mutable provenance: E. coli, methanoba trcol OB3b
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Other
role: SO:0000110
component: BBa_K1980006
component: BBa_K1980001
sequence annotation: BBa_K1980001
sequence annotation: BBa_K1980006
sequence: https://synbiohub.org/public/igem/BBa_K1980010_sequence/1

BBa_K1980006
type: Component
persistent identity: component2493041
display id: component2493041
version: 1
top level: BBa_K1980010
access: public
definition: https://synbiohub.org/public/igem/BBa_K1980006/1

BBa_K1980001
type: Component
persistent identity: component2493042
display id: component2493042
version: 1
top level: BBa_K1980010
access: public
definition: https://synbiohub.org/public/igem/BBa_K1980001/1

BBa_K1980001
type: SequenceAnnotation
persistent identity: annotation2493042
display id: annotation2493042
version: 1
top level: BBa_K1980010
location: https://synbiohub.org/public/igem/BBa_K1980010/annotation2493042/range2493042/1
component: BBa_K1980001

https://synbiohub.org/public/igem/BBa_K1980010/annotation2493042/range2493042/1
type: Range
persistent identity: range2493042
display id: range2493042
version: 1
direction: direction/reverse
top level: BBa_K1980010
start: 581
end: 1783
orientation: inline

BBa_K1980006
type: SequenceAnnotation
persistent identity: annotation2493041
display id: annotation2493041
version: 1
top level: BBa_K1980010
location: https://synbiohub.org/public/igem/BBa_K1980010/annotation2493041/range2493041/1
component: BBa_K1980006

https://synbiohub.org/public/igem/BBa_K1980010/annotation2493041/range2493041/1
type: Range
persistent identity: range2493041
display id: range2493041
version: 1
direction: direction/reverse
top level: BBa_K1980010
start: 1
end: 574
orientation: inline

BBa_K1122006
type: ComponentDefinition
persistent identity: BBa K1122006
display id: BBa_K1122006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: mCherry (BBa_J06504) compatible with Genabler assembly
creator: Lina Gasiūnaitė
created: 2013-08-12T11:00:00Z
modified: 2016-01-26T02:16:52Z
discontinued: false
dominant: false
group u list: _1433_
m user id: 4206
owner id: 18510
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: mCherry (BBa_J0654) part with inward facing EarI recognition sites at the ends compatible with Genabler assembly.
mutable notes: To be compatible with Genabler assembly, the part has inward-facing EarI sites at the ends. When assembled by BioBrick assembly, five N-terminal residues MetAlaSerSerSer- and one C-terminal Ala residue will be added to the original sequence of BBa_E0040.
mutable provenance: mCherry is based on the BioBrick BBa_J06504.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: start
sequence annotation: EarI site
sequence annotation: mCherry
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_K1122006_sequence/1

start
type: SequenceAnnotation
persistent identity: annotation2334604
display id: annotation2334604
version: 1
top level: BBa_K1122006
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1122006/annotation2334604/range2334604/1

https://synbiohub.org/public/igem/BBa_K1122006/annotation2334604/range2334604/1
type: Range
persistent identity: range2334604
display id: range2334604
version: 1
direction: direction/forward
top level: BBa_K1122006
start: 1
end: 3
orientation: inline

EarI site
type: SequenceAnnotation
persistent identity: annotation2334605
display id: annotation2334605
version: 1
top level: BBa_K1122006
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1122006/annotation2334605/range2334605/1

https://synbiohub.org/public/igem/BBa_K1122006/annotation2334605/range2334605/1
type: Range
persistent identity: range2334605
display id: range2334605
version: 1
direction: direction/forward
top level: BBa_K1122006
start: 6
end: 11
orientation: inline

mCherry
type: SequenceAnnotation
persistent identity: annotation2346320
display id: annotation2346320
version: 1
top level: BBa_K1122006
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1122006/annotation2346320/range2346320/1

https://synbiohub.org/public/igem/BBa_K1122006/annotation2346320/range2346320/1
type: Range
persistent identity: range2346320
display id: range2346320
version: 1
direction: direction/forward
top level: BBa_K1122006
start: 1
end: 727
orientation: inline

EarI site
type: SequenceAnnotation
persistent identity: annotation2334607
display id: annotation2334607
version: 1
top level: BBa_K1122006
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1122006/annotation2334607/range2334607/1

https://synbiohub.org/public/igem/BBa_K1122006/annotation2334607/range2334607/1
type: Range
persistent identity: range2334607
display id: range2334607
version: 1
direction: direction/reverse
top level: BBa_K1122006
start: 725
end: 730
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2334606
display id: annotation2334606
version: 1
top level: BBa_K1122006
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1122006/annotation2334606/range2334606/1

https://synbiohub.org/public/igem/BBa_K1122006/annotation2334606/range2334606/1
type: Range
persistent identity: range2334606
display id: range2334606
version: 1
direction: direction/forward
top level: BBa_K1122006
start: 724
end: 726
orientation: inline

BBa_K180010
type: ComponentDefinition
persistent identity: BBa K180010
display id: BBa_K180010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Rock-paper-scissors - Rock primary plasmid
creator: Richard Wilson, Stephanie Chambers
created: 2009-10-20T11:00:00Z
modified: 2015-05-08T01:11:06Z
discontinued: false
dominant: false
experience: experience/None
group u list: _278_
m user id: 0
owner id: 4082
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: Composite part, consisting of BBa_K180011 and BBa_K180012, but with BBa_K180011 reading along reverse strand, and BBa_K180012 reading along the forward strand. Forms the 'rock' primary plasmid in Team:Southampton's rock-paper-scissors project design.
mutable notes: This part has been specified as a basic BioBrick, due to some of the component parts reading in reverse (unable to specify using the component BioBrick format).
mutable provenance: See relevant biobrick component parts (BBa_K180011 and BBa_K180012).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: B0015
sequence annotation: lacIq
sequence annotation: C0171
sequence annotation: -10
sequence annotation: K180000
sequence annotation: Ptac
sequence annotation: K180008
sequence annotation: B0034
sequence annotation: K180011
sequence annotation: conserved
sequence annotation: lasI
sequence annotation: -35
sequence annotation: rhlR
sequence annotation: mCherry
sequence annotation: K180012
sequence annotation: C0178
sequence annotation: J23119
sequence: https://synbiohub.org/public/igem/BBa_K180010_sequence/1

B0015
type: SequenceAnnotation
persistent identity: annotation2058288
display id: annotation2058288
version: 1
top level: BBa_K180010
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K180010/annotation2058288/range2058288/1

https://synbiohub.org/public/igem/BBa_K180010/annotation2058288/range2058288/1
type: Range
persistent identity: range2058288
display id: range2058288
version: 1
direction: direction/reverse
top level: BBa_K180010
start: 1
end: 129
orientation: inline

lacIq
type: SequenceAnnotation
persistent identity: annotation2058308
display id: annotation2058308
version: 1
top level: BBa_K180010
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K180010/annotation2058308/range2058308/1

https://synbiohub.org/public/igem/BBa_K180010/annotation2058308/range2058308/1
type: Range
persistent identity: range2058308
display id: range2058308
version: 1
direction: direction/forward
top level: BBa_K180010
start: 936
end: 2017
orientation: inline

C0171
type: SequenceAnnotation
persistent identity: annotation2058289
display id: annotation2058289
version: 1
top level: BBa_K180010
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K180010/annotation2058289/range2058289/1

https://synbiohub.org/public/igem/BBa_K180010/annotation2058289/range2058289/1
type: Range
persistent identity: range2058289
display id: range2058289
version: 1
direction: direction/reverse
top level: BBa_K180010
start: 138
end: 866
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2058306
display id: annotation2058306
version: 1
top level: BBa_K180010
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K180010/annotation2058306/range2058306/1

https://synbiohub.org/public/igem/BBa_K180010/annotation2058306/range2058306/1
type: Range
persistent identity: range2058306
display id: range2058306
version: 1
direction: direction/reverse
top level: BBa_K180010
start: 899
end: 904
orientation: inline

K180000
type: SequenceAnnotation
persistent identity: annotation2058307
display id: annotation2058307
version: 1
top level: BBa_K180010
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K180010/annotation2058307/range2058307/1

https://synbiohub.org/public/igem/BBa_K180010/annotation2058307/range2058307/1
type: Range
persistent identity: range2058307
display id: range2058307
version: 1
direction: direction/forward
top level: BBa_K180010
start: 936
end: 2382
orientation: inline

Ptac
type: SequenceAnnotation
persistent identity: annotation2058309
display id: annotation2058309
version: 1
top level: BBa_K180010
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K180010/annotation2058309/range2058309/1

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type: Range
persistent identity: range2058309
display id: range2058309
version: 1
direction: direction/forward
top level: BBa_K180010
start: 2260
end: 2287
orientation: inline

K180008
type: SequenceAnnotation
persistent identity: annotation2058310
display id: annotation2058310
version: 1
top level: BBa_K180010
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K180010/annotation2058310/range2058310/1

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type: Range
persistent identity: range2058310
display id: range2058310
version: 1
direction: direction/forward
top level: BBa_K180010
start: 2409
end: 3116
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation2058292
display id: annotation2058292
version: 1
top level: BBa_K180010
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K180010/annotation2058292/range2058292/1

https://synbiohub.org/public/igem/BBa_K180010/annotation2058292/range2058292/1
type: Range
persistent identity: range2058292
display id: range2058292
version: 1
direction: direction/forward
top level: BBa_K180010
start: 3125
end: 3136
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation2058302
display id: annotation2058302
version: 1
top level: BBa_K180010
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K180010/annotation2058302/range2058302/1

https://synbiohub.org/public/igem/BBa_K180010/annotation2058302/range2058302/1
type: Range
persistent identity: range2058302
display id: range2058302
version: 1
direction: direction/reverse
top level: BBa_K180010
start: 873
end: 884
orientation: inline

K180011
type: SequenceAnnotation
persistent identity: annotation2058286
display id: annotation2058286
version: 1
top level: BBa_K180010
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K180010/annotation2058286/range2058286/1

https://synbiohub.org/public/igem/BBa_K180010/annotation2058286/range2058286/1
type: Range
persistent identity: range2058286
display id: range2058286
version: 1
direction: direction/reverse
top level: BBa_K180010
start: 1
end: 927
orientation: inline

conserved
type: SequenceAnnotation
persistent identity: annotation2058301
display id: annotation2058301
version: 1
top level: BBa_K180010
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K180010/annotation2058301/range2058301/1

https://synbiohub.org/public/igem/BBa_K180010/annotation2058301/range2058301/1
type: Range
persistent identity: range2058301
display id: range2058301
version: 1
direction: direction/forward
top level: BBa_K180010
start: 3129
end: 3132
orientation: inline

lasI
type: SequenceAnnotation
persistent identity: annotation2058314
display id: annotation2058314
version: 1
top level: BBa_K180010
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K180010/annotation2058314/range2058314/1

https://synbiohub.org/public/igem/BBa_K180010/annotation2058314/range2058314/1
type: Range
persistent identity: range2058314
display id: range2058314
version: 1
direction: direction/forward
top level: BBa_K180010
start: 3143
end: 3751
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2058305
display id: annotation2058305
version: 1
top level: BBa_K180010
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K180010/annotation2058305/range2058305/1

https://synbiohub.org/public/igem/BBa_K180010/annotation2058305/range2058305/1
type: Range
persistent identity: range2058305
display id: range2058305
version: 1
direction: direction/reverse
top level: BBa_K180010
start: 922
end: 927
orientation: inline

conserved
type: SequenceAnnotation
persistent identity: annotation2058303
display id: annotation2058303
version: 1
top level: BBa_K180010
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K180010/annotation2058303/range2058303/1

https://synbiohub.org/public/igem/BBa_K180010/annotation2058303/range2058303/1
type: Range
persistent identity: range2058303
display id: range2058303
version: 1
direction: direction/reverse
top level: BBa_K180010
start: 877
end: 880
orientation: inline

rhlR
type: SequenceAnnotation
persistent identity: annotation2058312
display id: annotation2058312
version: 1
top level: BBa_K180010
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K180010/annotation2058312/range2058312/1

https://synbiohub.org/public/igem/BBa_K180010/annotation2058312/range2058312/1
type: Range
persistent identity: range2058312
display id: range2058312
version: 1
direction: direction/reverse
top level: BBa_K180010
start: 138
end: 866
orientation: inline

conserved
type: SequenceAnnotation
persistent identity: annotation2058300
display id: annotation2058300
version: 1
top level: BBa_K180010
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K180010/annotation2058300/range2058300/1

https://synbiohub.org/public/igem/BBa_K180010/annotation2058300/range2058300/1
type: Range
persistent identity: range2058300
display id: range2058300
version: 1
direction: direction/forward
top level: BBa_K180010
start: 2395
end: 2398
orientation: inline

mCherry
type: SequenceAnnotation
persistent identity: annotation2058311
display id: annotation2058311
version: 1
top level: BBa_K180010
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K180010/annotation2058311/range2058311/1

https://synbiohub.org/public/igem/BBa_K180010/annotation2058311/range2058311/1
type: Range
persistent identity: range2058311
display id: range2058311
version: 1
direction: direction/forward
top level: BBa_K180010
start: 2409
end: 3116
orientation: inline

K180012
type: SequenceAnnotation
persistent identity: annotation2058287
display id: annotation2058287
version: 1
top level: BBa_K180010
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K180010/annotation2058287/range2058287/1

https://synbiohub.org/public/igem/BBa_K180010/annotation2058287/range2058287/1
type: Range
persistent identity: range2058287
display id: range2058287
version: 1
direction: direction/forward
top level: BBa_K180010
start: 936
end: 3888
orientation: inline

C0178
type: SequenceAnnotation
persistent identity: annotation2058313
display id: annotation2058313
version: 1
top level: BBa_K180010
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K180010/annotation2058313/range2058313/1

https://synbiohub.org/public/igem/BBa_K180010/annotation2058313/range2058313/1
type: Range
persistent identity: range2058313
display id: range2058313
version: 1
direction: direction/forward
top level: BBa_K180010
start: 3143
end: 3751
orientation: inline

B0015
type: SequenceAnnotation
persistent identity: annotation2058315
display id: annotation2058315
version: 1
top level: BBa_K180010
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K180010/annotation2058315/range2058315/1

https://synbiohub.org/public/igem/BBa_K180010/annotation2058315/range2058315/1
type: Range
persistent identity: range2058315
display id: range2058315
version: 1
direction: direction/forward
top level: BBa_K180010
start: 3760
end: 3888
orientation: inline

J23119
type: SequenceAnnotation
persistent identity: annotation2058304
display id: annotation2058304
version: 1
top level: BBa_K180010
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K180010/annotation2058304/range2058304/1

https://synbiohub.org/public/igem/BBa_K180010/annotation2058304/range2058304/1
type: Range
persistent identity: range2058304
display id: range2058304
version: 1
direction: direction/reverse
top level: BBa_K180010
start: 893
end: 927
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation2058291
display id: annotation2058291
version: 1
top level: BBa_K180010
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K180010/annotation2058291/range2058291/1

https://synbiohub.org/public/igem/BBa_K180010/annotation2058291/range2058291/1
type: Range
persistent identity: range2058291
display id: range2058291
version: 1
direction: direction/forward
top level: BBa_K180010
start: 2391
end: 2402
orientation: inline

BBa_K786010
type: ComponentDefinition
persistent identity: BBa K786010
display id: BBa_K786010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Gene expression for sensory rhodopsin I & II (SRI & SRII)
creator: Leung Wai Tak
created: 2012-09-20T11:00:00Z
modified: 2015-05-08T01:13:22Z
discontinued: false
dominant: false
group u list: _1041_
m user id: 0
owner id: 14285
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: The part contains OmpR, which is the promoter to sense activated sensory rhodopsin I & II (SRI & SRII) after light triggering.
mutable notes: Strong RBS was used to allow high expression of visible fluorescence protein once the sensory rhodopsin I & II (SRI & SRII) detect the correct light spectrum.
mutable provenance: All are constructed from previous biobricks
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_R0083
component: BBa_B0034
component: BBa_B0010
component: BBa_B0012
component: BBa_E1010
sequence annotation: BBa_B0034
sequence annotation: BBa_B0012
sequence annotation: BBa_R0083
sequence annotation: BBa_E1010
sequence annotation: BBa_B0010
sequence: https://synbiohub.org/public/igem/BBa_K786010_sequence/1

BBa_R0083
type: Component
persistent identity: component2259512
display id: component2259512
version: 1
top level: BBa_K786010
access: public
definition: https://synbiohub.org/public/igem/BBa_R0083/1

BBa_B0034
type: Component
persistent identity: component2259514
display id: component2259514
version: 1
top level: BBa_K786010
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0010
type: Component
persistent identity: component2259518
display id: component2259518
version: 1
top level: BBa_K786010
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
type: Component
persistent identity: component2259520
display id: component2259520
version: 1
top level: BBa_K786010
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_E1010
type: Component
persistent identity: component2259517
display id: component2259517
version: 1
top level: BBa_K786010
access: public
definition: mRFP1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2259514
display id: annotation2259514
version: 1
top level: BBa_K786010
location: https://synbiohub.org/public/igem/BBa_K786010/annotation2259514/range2259514/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K786010/annotation2259514/range2259514/1
type: Range
persistent identity: range2259514
display id: range2259514
version: 1
direction: direction/reverse
top level: BBa_K786010
start: 87
end: 98
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2259520
display id: annotation2259520
version: 1
top level: BBa_K786010
location: https://synbiohub.org/public/igem/BBa_K786010/annotation2259520/range2259520/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K786010/annotation2259520/range2259520/1
type: Range
persistent identity: range2259520
display id: range2259520
version: 1
direction: direction/forward
top level: BBa_K786010
start: 907
end: 947
orientation: inline

BBa_R0083
type: SequenceAnnotation
persistent identity: annotation2259512
display id: annotation2259512
version: 1
top level: BBa_K786010
location: https://synbiohub.org/public/igem/BBa_K786010/annotation2259512/range2259512/1
component: BBa_R0083

https://synbiohub.org/public/igem/BBa_K786010/annotation2259512/range2259512/1
type: Range
persistent identity: range2259512
display id: range2259512
version: 1
direction: direction/reverse
top level: BBa_K786010
start: 1
end: 78
orientation: inline

BBa_E1010
type: SequenceAnnotation
persistent identity: annotation2259517
display id: annotation2259517
version: 1
top level: BBa_K786010
location: https://synbiohub.org/public/igem/BBa_K786010/annotation2259517/range2259517/1
component: BBa_E1010

https://synbiohub.org/public/igem/BBa_K786010/annotation2259517/range2259517/1
type: Range
persistent identity: range2259517
display id: range2259517
version: 1
direction: direction/reverse
top level: BBa_K786010
start: 105
end: 810
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2259518
display id: annotation2259518
version: 1
top level: BBa_K786010
location: https://synbiohub.org/public/igem/BBa_K786010/annotation2259518/range2259518/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K786010/annotation2259518/range2259518/1
type: Range
persistent identity: range2259518
display id: range2259518
version: 1
direction: direction/forward
top level: BBa_K786010
start: 819
end: 898
orientation: inline

BBa_K1486005
type: ComponentDefinition
persistent identity: BBa K1486005
display id: BBa_K1486005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Arabinose promoter + sfGFP-CpxR [Cterm]
creator: iGem EPFL 2014
created: 2014-08-12T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part is composed of an arabinose-inducible promoter for superfolded GFP attached by a linker to CpxR (on the C terminus of CpxR).
mutable notes: We had to test both attachments, N and C terminus of the CpxR.
mutable provenance: The arabinose promoter and the sfGFP were obtained from the registry, while the CpxR was obtained from the E coli genome.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K1486000
component: BBa_B0034
component: BBa_K1486003
component: BBa_I0500
component: BBa_I746916
sequence annotation: BBa_K1486003
sequence annotation: BBa_I0500
sequence annotation: BBa_I746916
sequence annotation: BBa_K1486000
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K1486005_sequence/1

BBa_K1486000
type: Component
persistent identity: component2382088
display id: component2382088
version: 1
top level: BBa_K1486005
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486000/1

BBa_B0034
type: Component
persistent identity: component2382084
display id: component2382084
version: 1
top level: BBa_K1486005
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K1486003
type: Component
persistent identity: component2382089
display id: component2382089
version: 1
top level: BBa_K1486005
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486003/1

BBa_I0500
type: Component
persistent identity: component2382082
display id: component2382082
version: 1
top level: BBa_K1486005
access: public
definition: https://synbiohub.org/public/igem/BBa_I0500/1

BBa_I746916
type: Component
persistent identity: component2382093
display id: component2382093
version: 1
top level: BBa_K1486005
access: public
definition: https://synbiohub.org/public/igem/BBa_I746916/1

BBa_K1486003
type: SequenceAnnotation
persistent identity: annotation2382089
display id: annotation2382089
version: 1
top level: BBa_K1486005
location: https://synbiohub.org/public/igem/BBa_K1486005/annotation2382089/range2382089/1
component: BBa_K1486003

https://synbiohub.org/public/igem/BBa_K1486005/annotation2382089/range2382089/1
type: Range
persistent identity: range2382089
display id: range2382089
version: 1
direction: direction/reverse
top level: BBa_K1486005
start: 1925
end: 1948
orientation: inline

BBa_I0500
type: SequenceAnnotation
persistent identity: annotation2382082
display id: annotation2382082
version: 1
top level: BBa_K1486005
location: https://synbiohub.org/public/igem/BBa_K1486005/annotation2382082/range2382082/1
component: BBa_I0500

https://synbiohub.org/public/igem/BBa_K1486005/annotation2382082/range2382082/1
type: Range
persistent identity: range2382082
display id: range2382082
version: 1
direction: direction/reverse
top level: BBa_K1486005
start: 1
end: 1210
orientation: inline

BBa_I746916
type: SequenceAnnotation
persistent identity: annotation2382093
display id: annotation2382093
version: 1
top level: BBa_K1486005
location: https://synbiohub.org/public/igem/BBa_K1486005/annotation2382093/range2382093/1
component: BBa_I746916

https://synbiohub.org/public/igem/BBa_K1486005/annotation2382093/range2382093/1
type: Range
persistent identity: range2382093
display id: range2382093
version: 1
direction: direction/reverse
top level: BBa_K1486005
start: 1949
end: 2668
orientation: inline

BBa_K1486000
type: SequenceAnnotation
persistent identity: annotation2382088
display id: annotation2382088
version: 1
top level: BBa_K1486005
location: https://synbiohub.org/public/igem/BBa_K1486005/annotation2382088/range2382088/1
component: BBa_K1486000

https://synbiohub.org/public/igem/BBa_K1486005/annotation2382088/range2382088/1
type: Range
persistent identity: range2382088
display id: range2382088
version: 1
direction: direction/reverse
top level: BBa_K1486005
start: 1223
end: 1924
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2382084
display id: annotation2382084
version: 1
top level: BBa_K1486005
location: https://synbiohub.org/public/igem/BBa_K1486005/annotation2382084/range2382084/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1486005/annotation2382084/range2382084/1
type: Range
persistent identity: range2382084
display id: range2382084
version: 1
direction: direction/reverse
top level: BBa_K1486005
start: 1211
end: 1222
orientation: inline

BBa_K082003
type: ComponentDefinition
persistent identity: BBa K082003
display id: BBa_K082003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: GFP(+LVA)
creator: Yanting Xue , He Shen
created: 2008-10-27T12:00:00Z
modified: 2016-01-25T04:43:13Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _228_
m user id: 4206
owner id: 1560
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: noen
mutable notes: none
mutable provenance: none
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: GFP
sequence annotation: LVA
sequence: https://synbiohub.org/public/igem/BBa_K082003_sequence/1

GFP
type: SequenceAnnotation
persistent identity: annotation1992959
display id: annotation1992959
version: 1
top level: BBa_K082003
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K082003/annotation1992959/range1992959/1

https://synbiohub.org/public/igem/BBa_K082003/annotation1992959/range1992959/1
type: Range
persistent identity: range1992959
display id: range1992959
version: 1
direction: direction/forward
top level: BBa_K082003
start: 1
end: 720
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation1993327
display id: annotation1993327
version: 1
top level: BBa_K082003
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K082003/annotation1993327/range1993327/1

https://synbiohub.org/public/igem/BBa_K082003/annotation1993327/range1993327/1
type: Range
persistent identity: range1993327
display id: range1993327
version: 1
direction: direction/forward
top level: BBa_K082003
start: 721
end: 753
orientation: inline

mRFP1
type: ComponentDefinition
persistent identity: BBa K678032
display id: BBa_K678032
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: mRFP1-TS
creator: DTU-Denmark-2
created: 2011-09-08T11:00:00Z
modified: 2015-05-08T01:13:04Z
discontinued: false
dominant: false
experience: experience/None
group u list: _882_
m user id: 0
owner id: 8341
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Monomeric red fluorescent protein (mRFP1) is a derivative of the red fluorescent protein cloned from Discosoma coral (DsRed) (1). The successful use of mRFP1 in Aspergillus nidulans has previously been described (2).
mutable notes: This part was designed for the Plug'n'Play with DNA assembly standard (RFC 80), therefore this part contains tails at the 5' and 3' ends as shown on the figure below. Please notice that mRFP1-TS is not the same as BBa_K678030 or BBa_K678031.
mutable provenance: From p71-mRFP1 of the Center for Microbial Biotechnology plasmid collection at the Technical University of Denmark
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: Linker 3
sequence annotation: RFP
sequence: https://synbiohub.org/public/igem/BBa_K678032_sequence/1

Linker 3
type: SequenceAnnotation
persistent identity: annotation2127393
display id: annotation2127393
version: 1
top level: mRFP1
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678032/annotation2127393/range2127393/1

https://synbiohub.org/public/igem/BBa_K678032/annotation2127393/range2127393/1
type: Range
persistent identity: range2127393
display id: range2127393
version: 1
direction: direction/forward
top level: mRFP1
start: 1
end: 9
orientation: inline

RFP
type: SequenceAnnotation
persistent identity: annotation2127394
display id: annotation2127394
version: 1
top level: mRFP1
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K678032/annotation2127394/range2127394/1

https://synbiohub.org/public/igem/BBa_K678032/annotation2127394/range2127394/1
type: Range
persistent identity: range2127394
display id: range2127394
version: 1
direction: direction/forward
top level: mRFP1
start: 10
end: 687
orientation: inline

BBa_K1399021
type: ComponentDefinition
persistent identity: BBa K1399021
display id: BBa_K1399021
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ptet-GFP(LAA)
creator: Anna Stikane
created: 2014-09-18T11:00:00Z
modified: 2015-05-08T01:10:16Z
discontinued: false
dominant: false
group u list: _1777_
m user id: 0
owner id: 22477
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Tetracycline/aTc inducible promoter with GFP reporter tagged with LAA-ssrA degradation tag followed by terminator. The tagged GFP is actively degraded within cell, thus provides better temporal resolution of green fluorescence and promoter activity.
mutable notes: Part was assembled using BrickClip assembly (BBF RFC104) using other biobrick parts as templates. BrickClip assembly is a special case of more general Paperclip assembly method.[1]
mutable provenance: other parts
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_B0015
component: BBa_K1399006
component: BBa_B0034
component: BBa_R0040
sequence annotation: BBa_K1399006
sequence annotation: BBa_B0034
sequence annotation: BBa_B0015
sequence annotation: BBa_R0040
sequence: https://synbiohub.org/public/igem/BBa_K1399021_sequence/1

BBa_B0015
type: Component
persistent identity: component2384278
display id: component2384278
version: 1
top level: BBa_K1399021
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_K1399006
type: Component
persistent identity: component2384271
display id: component2384271
version: 1
top level: BBa_K1399021
access: public
definition: BBa_K1399006

BBa_B0034
type: Component
persistent identity: component2384265
display id: component2384265
version: 1
top level: BBa_K1399021
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_R0040
type: Component
persistent identity: component2384259
display id: component2384259
version: 1
top level: BBa_K1399021
access: public
definition: https://synbiohub.org/public/igem/BBa_R0040/1

BBa_K1399006
type: SequenceAnnotation
persistent identity: annotation2384271
display id: annotation2384271
version: 1
top level: BBa_K1399021
location: https://synbiohub.org/public/igem/BBa_K1399021/annotation2384271/range2384271/1
component: BBa_K1399006

https://synbiohub.org/public/igem/BBa_K1399021/annotation2384271/range2384271/1
type: Range
persistent identity: range2384271
display id: range2384271
version: 1
direction: direction/reverse
top level: BBa_K1399021
start: 81
end: 833
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2384265
display id: annotation2384265
version: 1
top level: BBa_K1399021
location: https://synbiohub.org/public/igem/BBa_K1399021/annotation2384265/range2384265/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1399021/annotation2384265/range2384265/1
type: Range
persistent identity: range2384265
display id: range2384265
version: 1
direction: direction/reverse
top level: BBa_K1399021
start: 63
end: 74
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2384278
display id: annotation2384278
version: 1
top level: BBa_K1399021
location: https://synbiohub.org/public/igem/BBa_K1399021/annotation2384278/range2384278/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K1399021/annotation2384278/range2384278/1
type: Range
persistent identity: range2384278
display id: range2384278
version: 1
direction: direction/reverse
top level: BBa_K1399021
start: 842
end: 970
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2384259
display id: annotation2384259
version: 1
top level: BBa_K1399021
location: https://synbiohub.org/public/igem/BBa_K1399021/annotation2384259/range2384259/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_K1399021/annotation2384259/range2384259/1
type: Range
persistent identity: range2384259
display id: range2384259
version: 1
direction: direction/forward
top level: BBa_K1399021
start: 1
end: 54
orientation: inline

BBa_K1184000
type: ComponentDefinition
persistent identity: BBa K1184000
display id: BBa_K1184000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: KillerRed
creator: Eric Pederson
created: 2013-09-03T11:00:00Z
modified: 2016-01-25T02:40:37Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1497_
m user id: 4206
owner id: 12713
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: KillerRed is a red fluorescent protein that produces reactive oxygen species (ROS) in the presence of yellow-orange light (540-585 nm). KillerRed is engineered from Aequorea victoria GFP (avGFP) to be phototoxic. It has been shown that KillerRed produces superoxide radical anions by reacting with water. Superoxide reacts with the chromophore of KillerRed, causing it to become dark, which ultimately gives rise to a bleaching effect. KillerRed is spectrally similar to mRFP1 with a similar brightness. KillerRed is oligomeric and may form large aggregates. This sequence is codon optimized for mammalian cells and has 14 rare proline codons for E. coli (CCC) and one rare arginine codon (AGA). Codon optimization should be taken into consideration if large amounts of the protein are required. Expression on a high-copy plasmid has produced detectable fluorescent signal, however.
mutable notes: This sequence is not codon optimized for E. coli.
mutable provenance: Engineered from avGFP. Provided by the Bruchez lab at Carnegie Mellon
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Double Stop
sequence annotation: cds
sequence annotation: Phototoxic Mutation Ala(163)
sequence annotation: Start
sequence annotation: Phototoxic Mutation Asn(147)
sequence annotation: Phototoxic Mutation Ser(121)
sequence annotation: Phototoxic Mutation Glu(70)
sequence annotation: Chromophore
sequence: https://synbiohub.org/public/igem/BBa_K1184000_sequence/1

Double Stop
type: SequenceAnnotation
persistent identity: annotation2334953
display id: annotation2334953
version: 1
top level: BBa_K1184000
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1184000/annotation2334953/range2334953/1

https://synbiohub.org/public/igem/BBa_K1184000/annotation2334953/range2334953/1
type: Range
persistent identity: range2334953
display id: range2334953
version: 1
direction: direction/forward
top level: BBa_K1184000
start: 718
end: 723
orientation: inline

cds
type: SequenceAnnotation
persistent identity: annotation2334955
display id: annotation2334955
version: 1
top level: BBa_K1184000
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1184000/annotation2334955/range2334955/1

https://synbiohub.org/public/igem/BBa_K1184000/annotation2334955/range2334955/1
type: Range
persistent identity: range2334955
display id: range2334955
version: 1
direction: direction/forward
top level: BBa_K1184000
start: 1
end: 717
orientation: inline

Phototoxic Mutation Ala(163)
type: SequenceAnnotation
persistent identity: annotation2334965
display id: annotation2334965
version: 1
top level: BBa_K1184000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1184000/annotation2334965/range2334965/1

https://synbiohub.org/public/igem/BBa_K1184000/annotation2334965/range2334965/1
type: Range
persistent identity: range2334965
display id: range2334965
version: 1
direction: direction/forward
top level: BBa_K1184000
start: 487
end: 489
orientation: inline

Start
type: SequenceAnnotation
persistent identity: annotation2334954
display id: annotation2334954
version: 1
top level: BBa_K1184000
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1184000/annotation2334954/range2334954/1

https://synbiohub.org/public/igem/BBa_K1184000/annotation2334954/range2334954/1
type: Range
persistent identity: range2334954
display id: range2334954
version: 1
direction: direction/forward
top level: BBa_K1184000
start: 1
end: 3
orientation: inline

Phototoxic Mutation Asn(147)
type: SequenceAnnotation
persistent identity: annotation2334960
display id: annotation2334960
version: 1
top level: BBa_K1184000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1184000/annotation2334960/range2334960/1

https://synbiohub.org/public/igem/BBa_K1184000/annotation2334960/range2334960/1
type: Range
persistent identity: range2334960
display id: range2334960
version: 1
direction: direction/forward
top level: BBa_K1184000
start: 439
end: 441
orientation: inline

Phototoxic Mutation Ser(121)
type: SequenceAnnotation
persistent identity: annotation2334957
display id: annotation2334957
version: 1
top level: BBa_K1184000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1184000/annotation2334957/range2334957/1

https://synbiohub.org/public/igem/BBa_K1184000/annotation2334957/range2334957/1
type: Range
persistent identity: range2334957
display id: range2334957
version: 1
direction: direction/forward
top level: BBa_K1184000
start: 367
end: 369
orientation: inline

Phototoxic Mutation Glu(70)
type: SequenceAnnotation
persistent identity: annotation2334956
display id: annotation2334956
version: 1
top level: BBa_K1184000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1184000/annotation2334956/range2334956/1

https://synbiohub.org/public/igem/BBa_K1184000/annotation2334956/range2334956/1
type: Range
persistent identity: range2334956
display id: range2334956
version: 1
direction: direction/forward
top level: BBa_K1184000
start: 208
end: 210
orientation: inline

Chromophore
type: SequenceAnnotation
persistent identity: annotation2334958
display id: annotation2334958
version: 1
top level: BBa_K1184000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1184000/annotation2334958/range2334958/1

https://synbiohub.org/public/igem/BBa_K1184000/annotation2334958/range2334958/1
type: Range
persistent identity: range2334958
display id: range2334958
version: 1
direction: direction/forward
top level: BBa_K1184000
start: 199
end: 207
orientation: inline

BBa_K1399005
type: ComponentDefinition
persistent identity: BBa K1399005
display id: BBa_K1399005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: GFP (mut3b) with AAV-ssrA degradation tag
creator: Anna Stikane
created: 2014-09-18T11:00:00Z
modified: 2015-05-08T01:10:16Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1777_
m user id: 0
owner id: 22477
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: GFP (mut3b) (see part BBa_E0040) with added AAV-ssrA degradation tag (part BBa_I11012). The tag increases RFP turn-over rate, thus providing better temporal resolution of red fluorescence. In the same time, maximal fluorescence amplitudes will be lower as newly formed protein is degraded as soon as it is formed. The tag encodes peptide sequence AANDENYAAAV and is recognized by ClpA and ClpX unfoldases and ClpX mediator SspB.[1] ClpA and ClpX then form a proteosome-like complex with ClpP protease and the protein is degraded.[1] The final three residues of the tag determines the strength of interaction with ClpX and thus the final protein degradation rate.[2] The AAV tag is reported to lead to reasonably fast protein degradation, degrading GFP with rate -0.012 per minute.[2] However, be aware that exact protein degradation rate depends on multiple factors: ClpXP and ClpAP protease and SspB mediator concentrations, protein stability, Km of binding to the protease, temperature [3]. References: [1] Flynn, J. M. et al. Overlapping recognition determinants within the ssrA degradation tag allow modulation of proteolysis. Proc. Natl. Acad. Sci. U. S. A. 98, 10584???9 (2001). [2] Andersen, J. B. et al. New unstable variants of green fluorescent protein for studies of transient gene expression in bacteria. Appl. Environ. Microbiol. 64, 2240???6 (1998). [3] Purcell, O., Grierson, C. S., Bernardo, M. Di & Savery, N. J. Temperature dependence of ssrA-tag mediated protein degradation. J. Biol. Eng. 6, 10 (2012).
mutable notes: The tag was attached to RFP using PCR and MABEL (mutagenesis with blunt-end ligation), thus avoiding introduction of additonal residues and restriction site. Different parts of the tag are recognized by different proteins, for example, the final 3 residues (AAV in this case) are recognised by ClpX, whereas first 4 residues of the tag are required for efficient SspB binding.[1] Thus modifications of these critical residues alter the efficacy with what different proteases bind to it.
mutable provenance: GFP comes from part BBa_E0040, tag sequence was obtained from part I11012, but same tag was also used in paper by Andersen et al., (1998).[2]
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: GFP (mut3b)
sequence annotation: stop
sequence annotation: start
sequence annotation: AAV-ssrA tag
sequence: https://synbiohub.org/public/igem/BBa_K1399005_sequence/1

GFP (mut3b)
type: SequenceAnnotation
persistent identity: annotation2383909
display id: annotation2383909
version: 1
top level: BBa_K1399005
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1399005/annotation2383909/range2383909/1

https://synbiohub.org/public/igem/BBa_K1399005/annotation2383909/range2383909/1
type: Range
persistent identity: range2383909
display id: range2383909
version: 1
direction: direction/forward
top level: BBa_K1399005
start: 4
end: 714
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2383911
display id: annotation2383911
version: 1
top level: BBa_K1399005
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1399005/annotation2383911/range2383911/1

https://synbiohub.org/public/igem/BBa_K1399005/annotation2383911/range2383911/1
type: Range
persistent identity: range2383911
display id: range2383911
version: 1
direction: direction/forward
top level: BBa_K1399005
start: 748
end: 750
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2383912
display id: annotation2383912
version: 1
top level: BBa_K1399005
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1399005/annotation2383912/range2383912/1

https://synbiohub.org/public/igem/BBa_K1399005/annotation2383912/range2383912/1
type: Range
persistent identity: range2383912
display id: range2383912
version: 1
direction: direction/forward
top level: BBa_K1399005
start: 751
end: 753
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2383908
display id: annotation2383908
version: 1
top level: BBa_K1399005
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1399005/annotation2383908/range2383908/1

https://synbiohub.org/public/igem/BBa_K1399005/annotation2383908/range2383908/1
type: Range
persistent identity: range2383908
display id: range2383908
version: 1
direction: direction/forward
top level: BBa_K1399005
start: 1
end: 3
orientation: inline

AAV-ssrA tag
type: SequenceAnnotation
persistent identity: annotation2383910
display id: annotation2383910
version: 1
top level: BBa_K1399005
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1399005/annotation2383910/range2383910/1

https://synbiohub.org/public/igem/BBa_K1399005/annotation2383910/range2383910/1
type: Range
persistent identity: range2383910
display id: range2383910
version: 1
direction: direction/forward
top level: BBa_K1399005
start: 715
end: 747
orientation: inline

BBa_K993000
type: ComponentDefinition
persistent identity: BBa K993000
display id: BBa_K993000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RFP Generator with ROSE thermometer
creator: ABD??LKADİR KARADAĞ
created: 2013-06-13T11:00:00Z
modified: 2015-05-08T01:13:51Z
discontinued: false
dominant: false
group u list: _1290_
m user id: 0
owner id: 11156
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: The repression of heat shock gene expression (ROSE) element is an RNA element found in the 5' UTR of some heat shock protein's mRNAs. The ROSE element is an RNA thermometer that negatively regulates heat shock gene expression. The secondary structure is thought to be altered by temperature, thus it is an RNA thermometer. This structure blocks access to the ribosome binding site at normal temperatures. During heat shock however, the structure changes freeing the ribosome binding site and allowing expression to occur. RNA Thermometers are the only known single-component regulators of gene expression.(1) RNA Thermometers are thermosensors that regulate gene expression by temperature-induced changes in RNA conformation. Naturally occurring RNA Thermometers exhibit complex secondary structures which are believed to undergo a series of gradual structural changes in response to temperature shifts.(2) RNA Thermometers often regulate genes required during either a heat shock or cold shock response, but have been implicated in other regulatory roles such as in pathogenecity and starvation.(3)
mutable notes: Note that RNA thermometer actually works as an RBS sequence. Therefore, this part does not include an additional ribosome binding site. Also, this part does not contain an terminator sequence, but a simple stop codon. The RFP should be translated continuously.
mutable provenance: Bradyrhizobium japonicum USDA 110 and Registry
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
sequence annotation: pVeg2
sequence annotation: RFP Generator
sequence annotation: Start Codon
sequence annotation: Finish Codon
sequence annotation: ROSE Thermometer
sequence: https://synbiohub.org/public/igem/BBa_K993000_sequence/1

pVeg2
type: SequenceAnnotation
persistent identity: annotation2329453
display id: annotation2329453
version: 1
top level: BBa_K993000
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K993000/annotation2329453/range2329453/1

https://synbiohub.org/public/igem/BBa_K993000/annotation2329453/range2329453/1
type: Range
persistent identity: range2329453
display id: range2329453
version: 1
direction: direction/forward
top level: BBa_K993000
start: 1
end: 97
orientation: inline

RFP Generator
type: SequenceAnnotation
persistent identity: annotation2329455
display id: annotation2329455
version: 1
top level: BBa_K993000
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K993000/annotation2329455/range2329455/1

https://synbiohub.org/public/igem/BBa_K993000/annotation2329455/range2329455/1
type: Range
persistent identity: range2329455
display id: range2329455
version: 1
direction: direction/forward
top level: BBa_K993000
start: 194
end: 902
orientation: inline

Start Codon
type: SequenceAnnotation
persistent identity: annotation2329457
display id: annotation2329457
version: 1
top level: BBa_K993000
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K993000/annotation2329457/range2329457/1

https://synbiohub.org/public/igem/BBa_K993000/annotation2329457/range2329457/1
type: Range
persistent identity: range2329457
display id: range2329457
version: 1
direction: direction/forward
top level: BBa_K993000
start: 194
end: 196
orientation: inline

Finish Codon
type: SequenceAnnotation
persistent identity: annotation2329456
display id: annotation2329456
version: 1
top level: BBa_K993000
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K993000/annotation2329456/range2329456/1

https://synbiohub.org/public/igem/BBa_K993000/annotation2329456/range2329456/1
type: Range
persistent identity: range2329456
display id: range2329456
version: 1
direction: direction/forward
top level: BBa_K993000
start: 900
end: 902
orientation: inline

ROSE Thermometer
type: SequenceAnnotation
persistent identity: annotation2329454
display id: annotation2329454
version: 1
top level: BBa_K993000
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K993000/annotation2329454/range2329454/1

https://synbiohub.org/public/igem/BBa_K993000/annotation2329454/range2329454/1
type: Range
persistent identity: range2329454
display id: range2329454
version: 1
direction: direction/forward
top level: BBa_K993000
start: 98
end: 193
orientation: inline

BBa_K748005
type: ComponentDefinition
persistent identity: BBa K748005
display id: BBa_K748005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: P2+RBS+GFP+T. P2 can be activated by phosphorylated AgrA.
creator: Lei Qiao
created: 2012-09-14T11:00:00Z
modified: 2015-05-08T01:13:12Z
discontinued: false
dominant: false
experience: experience/None
group u list: _999_
m user id: 0
owner id: 8564
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: P2 can be activated by phosphorylated AgrA, leading to the production of GFP and 3OC6HSL. Thus we can detect the presence of S.aureus expediently by observing the expression of GFP.
mutable notes: nothing special
mutable provenance: de novo synthesis
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_B0034
component: BBa_E0040
component: BBa_K748008
component: BBa_B0015
sequence annotation: BBa_B0015
sequence annotation: BBa_E0040
sequence annotation: BBa_B0034
sequence annotation: BBa_K748008
sequence: https://synbiohub.org/public/igem/BBa_K748005_sequence/1

BBa_B0034
type: Component
persistent identity: component2183471
display id: component2183471
version: 1
top level: BBa_K748005
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_E0040
type: Component
persistent identity: component2183473
display id: component2183473
version: 1
top level: BBa_K748005
access: public
definition: GFP

BBa_K748008
type: Component
persistent identity: component2183469
display id: component2183469
version: 1
top level: BBa_K748005
access: public
definition: https://synbiohub.org/public/igem/BBa_K748008/1

BBa_B0015
type: Component
persistent identity: component2183480
display id: component2183480
version: 1
top level: BBa_K748005
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2183480
display id: annotation2183480
version: 1
top level: BBa_K748005
location: https://synbiohub.org/public/igem/BBa_K748005/annotation2183480/range2183480/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K748005/annotation2183480/range2183480/1
type: Range
persistent identity: range2183480
display id: range2183480
version: 1
direction: direction/reverse
top level: BBa_K748005
start: 850
end: 978
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation2183473
display id: annotation2183473
version: 1
top level: BBa_K748005
location: https://synbiohub.org/public/igem/BBa_K748005/annotation2183473/range2183473/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_K748005/annotation2183473/range2183473/1
type: Range
persistent identity: range2183473
display id: range2183473
version: 1
direction: direction/reverse
top level: BBa_K748005
start: 122
end: 841
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2183471
display id: annotation2183471
version: 1
top level: BBa_K748005
location: https://synbiohub.org/public/igem/BBa_K748005/annotation2183471/range2183471/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K748005/annotation2183471/range2183471/1
type: Range
persistent identity: range2183471
display id: range2183471
version: 1
direction: direction/reverse
top level: BBa_K748005
start: 104
end: 115
orientation: inline

BBa_K748008
type: SequenceAnnotation
persistent identity: annotation2183469
display id: annotation2183469
version: 1
top level: BBa_K748005
location: https://synbiohub.org/public/igem/BBa_K748005/annotation2183469/range2183469/1
component: BBa_K748008

https://synbiohub.org/public/igem/BBa_K748005/annotation2183469/range2183469/1
type: Range
persistent identity: range2183469
display id: range2183469
version: 1
direction: direction/reverse
top level: BBa_K748005
start: 1
end: 95
orientation: inline

BBa_K1122005
type: ComponentDefinition
persistent identity: BBa K1122005
display id: BBa_K1122005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: dsRED (BBa_E1010) compatible with Genabler assembly
creator: Lina Gasiūnaitė
created: 2013-08-12T11:00:00Z
modified: 2016-01-26T02:16:37Z
discontinued: false
dominant: false
group u list: _1433_
m user id: 4206
owner id: 18510
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: dsRED (BBa_E1010) part with inward facing EarI recognition sites at the ends
mutable notes: To be compatible with Genabler assembly, the part has inward-facing EarI sites at the ends. When assembled by BioBrick assembly, five N-terminal residues MetAlaSerSerSer- and one C-terminal Ala residue will be added to the original sequence of BBa_E1010.
mutable provenance: dsRED is based on BBa_E1010
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: start
sequence annotation: EarI site
sequence annotation: stop
sequence annotation: dsRED
sequence: https://synbiohub.org/public/igem/BBa_K1122005_sequence/1

start
type: SequenceAnnotation
persistent identity: annotation2334600
display id: annotation2334600
version: 1
top level: BBa_K1122005
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1122005/annotation2334600/range2334600/1

https://synbiohub.org/public/igem/BBa_K1122005/annotation2334600/range2334600/1
type: Range
persistent identity: range2334600
display id: range2334600
version: 1
direction: direction/forward
top level: BBa_K1122005
start: 1
end: 3
orientation: inline

EarI site
type: SequenceAnnotation
persistent identity: annotation2334602
display id: annotation2334602
version: 1
top level: BBa_K1122005
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1122005/annotation2334602/range2334602/1

https://synbiohub.org/public/igem/BBa_K1122005/annotation2334602/range2334602/1
type: Range
persistent identity: range2334602
display id: range2334602
version: 1
direction: direction/reverse
top level: BBa_K1122005
start: 692
end: 697
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2334603
display id: annotation2334603
version: 1
top level: BBa_K1122005
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1122005/annotation2334603/range2334603/1

https://synbiohub.org/public/igem/BBa_K1122005/annotation2334603/range2334603/1
type: Range
persistent identity: range2334603
display id: range2334603
version: 1
direction: direction/forward
top level: BBa_K1122005
start: 691
end: 693
orientation: inline

dsRED
type: SequenceAnnotation
persistent identity: annotation2346297
display id: annotation2346297
version: 1
top level: BBa_K1122005
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1122005/annotation2346297/range2346297/1

https://synbiohub.org/public/igem/BBa_K1122005/annotation2346297/range2346297/1
type: Range
persistent identity: range2346297
display id: range2346297
version: 1
direction: direction/forward
top level: BBa_K1122005
start: 1
end: 697
orientation: inline

EarI site
type: SequenceAnnotation
persistent identity: annotation2334601
display id: annotation2334601
version: 1
top level: BBa_K1122005
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1122005/annotation2334601/range2334601/1

https://synbiohub.org/public/igem/BBa_K1122005/annotation2334601/range2334601/1
type: Range
persistent identity: range2334601
display id: range2334601
version: 1
direction: direction/forward
top level: BBa_K1122005
start: 6
end: 11
orientation: inline

BBa_K1679005
type: ComponentDefinition
persistent identity: BBa K1679005
display id: BBa_K1679005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: GFP Reporter
creator: Qikai Qin
created: 2015-09-15T11:00:00Z
modified: 2015-09-18T08:49:20Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2097_
m user id: 21726
owner id: 21726
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This is a device that contain promoter BBa_J23117, RiboJ and BBa_I13504.
mutable notes: We want to test the strength of the J23117 without disturbance from genetic context.
mutable provenance: BBa_J23101 RiboJ BBa_I13504
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_J23117
component: BBa_K1679038
component: BBa_I13504
sequence annotation: BBa_I13504
sequence annotation: BBa_K1679038
sequence annotation: BBa_J23117
sequence: https://synbiohub.org/public/igem/BBa_K1679005_sequence/1

BBa_J23117
type: Component
persistent identity: component2474712
display id: component2474712
version: 1
top level: BBa_K1679005
access: public
definition: https://synbiohub.org/public/igem/BBa_J23117/1

BBa_K1679038
type: Component
persistent identity: component2474713
display id: component2474713
version: 1
top level: BBa_K1679005
access: public
definition: https://synbiohub.org/public/igem/BBa_K1679038/1

BBa_I13504
type: Component
persistent identity: component2474723
display id: component2474723
version: 1
top level: BBa_K1679005
access: public
definition: https://synbiohub.org/public/igem/BBa_I13504/1

BBa_I13504
type: SequenceAnnotation
persistent identity: annotation2474723
display id: annotation2474723
version: 1
top level: BBa_K1679005
location: https://synbiohub.org/public/igem/BBa_K1679005/annotation2474723/range2474723/1
component: BBa_I13504

https://synbiohub.org/public/igem/BBa_K1679005/annotation2474723/range2474723/1
type: Range
persistent identity: range2474723
display id: range2474723
version: 1
direction: direction/reverse
top level: BBa_K1679005
start: 127
end: 1001
orientation: inline

BBa_K1679038
type: SequenceAnnotation
persistent identity: annotation2474713
display id: annotation2474713
version: 1
top level: BBa_K1679005
location: https://synbiohub.org/public/igem/BBa_K1679005/annotation2474713/range2474713/1
component: BBa_K1679038

https://synbiohub.org/public/igem/BBa_K1679005/annotation2474713/range2474713/1
type: Range
persistent identity: range2474713
display id: range2474713
version: 1
direction: direction/reverse
top level: BBa_K1679005
start: 44
end: 118
orientation: inline

BBa_J23117
type: SequenceAnnotation
persistent identity: annotation2474712
display id: annotation2474712
version: 1
top level: BBa_K1679005
location: https://synbiohub.org/public/igem/BBa_K1679005/annotation2474712/range2474712/1
component: BBa_J23117

https://synbiohub.org/public/igem/BBa_K1679005/annotation2474712/range2474712/1
type: Range
persistent identity: range2474712
display id: range2474712
version: 1
direction: direction/reverse
top level: BBa_K1679005
start: 1
end: 35
orientation: inline

GFP_Freibu
type: ComponentDefinition
persistent identity: BBa K863120
display id: BBa_K863120
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (Freiburg-Standard)
creator: Moritz M??ller
created: 2012-09-19T11:00:00Z
modified: 2016-01-25T01:32:59Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1123_
m user id: 4206
owner id: 13080
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Untagged version of gfp from Repressilator reporter. See the [http://partsregistry.org/wiki/index.php?title=Part:BBa_E0040 untagged version] for source information.
mutable notes: Added Freiburg-restriction-sites for in frame assembly.
mutable provenance: [http://partsregistry.org/Part:BBa_I13522 BBa_I13522]
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: NgoMIV
sequence annotation: BBa_E0040
sequence annotation: ATG
sequence annotation: TAA
sequence annotation: AgeI
sequence: https://synbiohub.org/public/igem/BBa_K863120_sequence/1

NgoMIV
type: SequenceAnnotation
persistent identity: annotation2187770
display id: annotation2187770
version: 1
top level: GFP_Freibu
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K863120/annotation2187770/range2187770/1

https://synbiohub.org/public/igem/BBa_K863120/annotation2187770/range2187770/1
type: Range
persistent identity: range2187770
display id: range2187770
version: 1
direction: direction/forward
top level: GFP_Freibu
start: 4
end: 11
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation2187773
display id: annotation2187773
version: 1
top level: GFP_Freibu
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K863120/annotation2187773/range2187773/1

https://synbiohub.org/public/igem/BBa_K863120/annotation2187773/range2187773/1
type: Range
persistent identity: range2187773
display id: range2187773
version: 1
direction: direction/forward
top level: GFP_Freibu
start: 12
end: 720
orientation: inline

ATG
type: SequenceAnnotation
persistent identity: annotation2187769
display id: annotation2187769
version: 1
top level: GFP_Freibu
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K863120/annotation2187769/range2187769/1

https://synbiohub.org/public/igem/BBa_K863120/annotation2187769/range2187769/1
type: Range
persistent identity: range2187769
display id: range2187769
version: 1
direction: direction/forward
top level: GFP_Freibu
start: 1
end: 3
orientation: inline

TAA
type: SequenceAnnotation
persistent identity: annotation2187771
display id: annotation2187771
version: 1
top level: GFP_Freibu
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K863120/annotation2187771/range2187771/1

https://synbiohub.org/public/igem/BBa_K863120/annotation2187771/range2187771/1
type: Range
persistent identity: range2187771
display id: range2187771
version: 1
direction: direction/forward
top level: GFP_Freibu
start: 727
end: 729
orientation: inline

AgeI
type: SequenceAnnotation
persistent identity: annotation2187772
display id: annotation2187772
version: 1
top level: GFP_Freibu
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K863120/annotation2187772/range2187772/1

https://synbiohub.org/public/igem/BBa_K863120/annotation2187772/range2187772/1
type: Range
persistent identity: range2187772
display id: range2187772
version: 1
direction: direction/forward
top level: GFP_Freibu
start: 721
end: 726
orientation: inline

BBa_K1033908
type: ComponentDefinition
persistent identity: BBa K1033908
display id: BBa_K1033908
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: fwYellow, yellow chromoprotein (incl RBS, J23106)
creator: Sabri Jamal
created: 2013-09-16T11:00:00Z
modified: 2015-05-08T01:08:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1340_
m user id: 0
owner id: 13997
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This chromoprotein naturally exhibits strong color when expressed.
mutable notes: Cloned from FezziwigYFP vector, DNA 2.0.
mutable provenance: Synthetic chromoprotein from DNA 2.0.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_K1033909
component: BBa_J23106
sequence annotation: BBa_K1033909
sequence annotation: BBa_J23106
sequence: https://synbiohub.org/public/igem/BBa_K1033908_sequence/1

BBa_K1033909
type: Component
persistent identity: component2371223
display id: component2371223
version: 1
top level: BBa_K1033908
access: public
definition: fwYellow

BBa_J23106
type: Component
persistent identity: component2371219
display id: component2371219
version: 1
top level: BBa_K1033908
access: public
definition: https://synbiohub.org/public/igem/BBa_J23106/1

BBa_K1033909
type: SequenceAnnotation
persistent identity: annotation2371223
display id: annotation2371223
version: 1
top level: BBa_K1033908
location: https://synbiohub.org/public/igem/BBa_K1033908/annotation2371223/range2371223/1
component: BBa_K1033909

https://synbiohub.org/public/igem/BBa_K1033908/annotation2371223/range2371223/1
type: Range
persistent identity: range2371223
display id: range2371223
version: 1
direction: direction/reverse
top level: BBa_K1033908
start: 44
end: 775
orientation: inline

BBa_J23106
type: SequenceAnnotation
persistent identity: annotation2371219
display id: annotation2371219
version: 1
top level: BBa_K1033908
location: https://synbiohub.org/public/igem/BBa_K1033908/annotation2371219/range2371219/1
component: BBa_J23106

https://synbiohub.org/public/igem/BBa_K1033908/annotation2371219/range2371219/1
type: Range
persistent identity: range2371219
display id: range2371219
version: 1
direction: direction/reverse
top level: BBa_K1033908
start: 1
end: 35
orientation: inline

Tm-nCFP
type: ComponentDefinition
persistent identity: BBa K157017
display id: BBa_K157017
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Tm-nCFP
creator: Freiburg iGEM Team 08
created: 2008-10-25T11:00:00Z
modified: 2015-05-08T01:10:54Z
discontinued: false
dominant: false
experience: experience/None
group u list: _232_
m user id: 0
owner id: 1673
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: N-terminal part of the Split-fluorophore CFP, fused to the trans-membrane region of the Erbb1/EGF-receptor.
mutable notes: none
mutable provenance: Derived by cloning parts BBa_K157002, BBa_K157006. For further information see the description of the parts mentioned above.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: Tm
sequence annotation: scar (Thr, Gly)
sequence annotation: N-Term_CFP
sequence annotation: AgeI
sequence annotation: NgoMI
sequence annotation: Tm-nCFP
sequence: https://synbiohub.org/public/igem/BBa_K157017_sequence/1

Tm
type: SequenceAnnotation
persistent identity: annotation1991285
display id: annotation1991285
version: 1
top level: Tm-nCFP
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157017/annotation1991285/range1991285/1

https://synbiohub.org/public/igem/BBa_K157017/annotation1991285/range1991285/1
type: Range
persistent identity: range1991285
display id: range1991285
version: 1
direction: direction/forward
top level: Tm-nCFP
start: 10
end: 78
orientation: inline

scar (Thr, Gly)
type: SequenceAnnotation
persistent identity: annotation1991286
display id: annotation1991286
version: 1
top level: Tm-nCFP
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157017/annotation1991286/range1991286/1

https://synbiohub.org/public/igem/BBa_K157017/annotation1991286/range1991286/1
type: Range
persistent identity: range1991286
display id: range1991286
version: 1
direction: direction/forward
top level: Tm-nCFP
start: 79
end: 84
orientation: inline

N-Term_CFP
type: SequenceAnnotation
persistent identity: annotation1991287
display id: annotation1991287
version: 1
top level: Tm-nCFP
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157017/annotation1991287/range1991287/1

https://synbiohub.org/public/igem/BBa_K157017/annotation1991287/range1991287/1
type: Range
persistent identity: range1991287
display id: range1991287
version: 1
direction: direction/forward
top level: Tm-nCFP
start: 85
end: 549
orientation: inline

AgeI
type: SequenceAnnotation
persistent identity: annotation1989978
display id: annotation1989978
version: 1
top level: Tm-nCFP
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157017/annotation1989978/range1989978/1

https://synbiohub.org/public/igem/BBa_K157017/annotation1989978/range1989978/1
type: Range
persistent identity: range1989978
display id: range1989978
version: 1
direction: direction/forward
top level: Tm-nCFP
start: 550
end: 555
orientation: inline

NgoMI
type: SequenceAnnotation
persistent identity: annotation1989977
display id: annotation1989977
version: 1
top level: Tm-nCFP
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157017/annotation1989977/range1989977/1

https://synbiohub.org/public/igem/BBa_K157017/annotation1989977/range1989977/1
type: Range
persistent identity: range1989977
display id: range1989977
version: 1
direction: direction/forward
top level: Tm-nCFP
start: 4
end: 9
orientation: inline

Tm-nCFP
type: SequenceAnnotation
persistent identity: annotation1989979
display id: annotation1989979
version: 1
top level: Tm-nCFP
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K157017/annotation1989979/range1989979/1

https://synbiohub.org/public/igem/BBa_K157017/annotation1989979/range1989979/1
type: Range
persistent identity: range1989979
display id: range1989979
version: 1
direction: direction/forward
top level: Tm-nCFP
start: 1
end: 555
orientation: inline

CBDcex(J61
type: ComponentDefinition
persistent identity: BBa K863104
display id: BBa_K863104
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Cellulose binding Domain from C. Fimi with const. Promotor and GFP
creator: Moritz M??ller
created: 2012-09-20T11:00:00Z
modified: 2015-06-17T01:19:39Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1123_
m user id: 4206
owner id: 13080
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Cellulose binding Domain from Cellulomonas Fimi with const. Promotor J61101 and Reporter GFP
mutable notes: a switch to a const. Promoter
mutable provenance: different
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: BBa_E0040
sequence annotation: BBa_J23100
sequence annotation: Freiburg-Scar
sequence annotation: ATG
sequence annotation: AgeI
sequence annotation: BBa_J61101
sequence annotation: Exoglucanase
sequence annotation: BBa_K863121
sequence annotation: BBa_K863101
sequence annotation: His-Tag
sequence annotation: NgoMIV
sequence annotation: GS-Linker
sequence annotation: Cellulose binding domain (CBDcex)
sequence annotation: TAA
sequence: https://synbiohub.org/public/igem/BBa_K863104_sequence/1

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation2189494
display id: annotation2189494
version: 1
top level: CBDcex(J61
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K863104/annotation2189494/range2189494/1

https://synbiohub.org/public/igem/BBa_K863104/annotation2189494/range2189494/1
type: Range
persistent identity: range2189494
display id: range2189494
version: 1
direction: direction/forward
top level: CBDcex(J61
start: 404
end: 1117
orientation: inline

BBa_J23100
type: SequenceAnnotation
persistent identity: annotation2189483
display id: annotation2189483
version: 1
top level: CBDcex(J61
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K863104/annotation2189483/range2189483/1

https://synbiohub.org/public/igem/BBa_K863104/annotation2189483/range2189483/1
type: Range
persistent identity: range2189483
display id: range2189483
version: 1
direction: direction/forward
top level: CBDcex(J61
start: 1
end: 35
orientation: inline

Freiburg-Scar
type: SequenceAnnotation
persistent identity: annotation2189492
display id: annotation2189492
version: 1
top level: CBDcex(J61
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K863104/annotation2189492/range2189492/1

https://synbiohub.org/public/igem/BBa_K863104/annotation2189492/range2189492/1
type: Range
persistent identity: range2189492
display id: range2189492
version: 1
direction: direction/forward
top level: CBDcex(J61
start: 398
end: 403
orientation: inline

ATG
type: SequenceAnnotation
persistent identity: annotation2189485
display id: annotation2189485
version: 1
top level: CBDcex(J61
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K863104/annotation2189485/range2189485/1

https://synbiohub.org/public/igem/BBa_K863104/annotation2189485/range2189485/1
type: Range
persistent identity: range2189485
display id: range2189485
version: 1
direction: direction/forward
top level: CBDcex(J61
start: 62
end: 64
orientation: inline

AgeI
type: SequenceAnnotation
persistent identity: annotation2189496
display id: annotation2189496
version: 1
top level: CBDcex(J61
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K863104/annotation2189496/range2189496/1

https://synbiohub.org/public/igem/BBa_K863104/annotation2189496/range2189496/1
type: Range
persistent identity: range2189496
display id: range2189496
version: 1
direction: direction/forward
top level: CBDcex(J61
start: 1136
end: 1141
orientation: inline

BBa_J61101
type: SequenceAnnotation
persistent identity: annotation2189484
display id: annotation2189484
version: 1
top level: CBDcex(J61
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K863104/annotation2189484/range2189484/1

https://synbiohub.org/public/igem/BBa_K863104/annotation2189484/range2189484/1
type: Range
persistent identity: range2189484
display id: range2189484
version: 1
direction: direction/forward
top level: CBDcex(J61
start: 44
end: 55
orientation: inline

Exoglucanase
type: SequenceAnnotation
persistent identity: annotation2189488
display id: annotation2189488
version: 1
top level: CBDcex(J61
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K863104/annotation2189488/range2189488/1

https://synbiohub.org/public/igem/BBa_K863104/annotation2189488/range2189488/1
type: Range
persistent identity: range2189488
display id: range2189488
version: 1
direction: direction/forward
top level: CBDcex(J61
start: 71
end: 82
orientation: inline

BBa_K863121
type: SequenceAnnotation
persistent identity: annotation2189493
display id: annotation2189493
version: 1
top level: CBDcex(J61
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K863104/annotation2189493/range2189493/1

https://synbiohub.org/public/igem/BBa_K863104/annotation2189493/range2189493/1
type: Range
persistent identity: range2189493
display id: range2189493
version: 1
direction: direction/forward
top level: CBDcex(J61
start: 404
end: 1144
orientation: inline

Exoglucanase
type: SequenceAnnotation
persistent identity: annotation2189490
display id: annotation2189490
version: 1
top level: CBDcex(J61
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K863104/annotation2189490/range2189490/1

https://synbiohub.org/public/igem/BBa_K863104/annotation2189490/range2189490/1
type: Range
persistent identity: range2189490
display id: range2189490
version: 1
direction: direction/forward
top level: CBDcex(J61
start: 383
end: 391
orientation: inline

BBa_K863101
type: SequenceAnnotation
persistent identity: annotation2189486
display id: annotation2189486
version: 1
top level: CBDcex(J61
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K863104/annotation2189486/range2189486/1

https://synbiohub.org/public/igem/BBa_K863104/annotation2189486/range2189486/1
type: Range
persistent identity: range2189486
display id: range2189486
version: 1
direction: direction/forward
top level: CBDcex(J61
start: 62
end: 397
orientation: inline

His-Tag
type: SequenceAnnotation
persistent identity: annotation2189495
display id: annotation2189495
version: 1
top level: CBDcex(J61
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K863104/annotation2189495/range2189495/1

https://synbiohub.org/public/igem/BBa_K863104/annotation2189495/range2189495/1
type: Range
persistent identity: range2189495
display id: range2189495
version: 1
direction: direction/forward
top level: CBDcex(J61
start: 1118
end: 1135
orientation: inline

NgoMIV
type: SequenceAnnotation
persistent identity: annotation2189487
display id: annotation2189487
version: 1
top level: CBDcex(J61
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K863104/annotation2189487/range2189487/1

https://synbiohub.org/public/igem/BBa_K863104/annotation2189487/range2189487/1
type: Range
persistent identity: range2189487
display id: range2189487
version: 1
direction: direction/forward
top level: CBDcex(J61
start: 65
end: 70
orientation: inline

GS-Linker
type: SequenceAnnotation
persistent identity: annotation2189491
display id: annotation2189491
version: 1
top level: CBDcex(J61
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K863104/annotation2189491/range2189491/1

https://synbiohub.org/public/igem/BBa_K863104/annotation2189491/range2189491/1
type: Range
persistent identity: range2189491
display id: range2189491
version: 1
direction: direction/forward
top level: CBDcex(J61
start: 392
end: 397
orientation: inline

Cellulose binding domain (CBDcex)
type: SequenceAnnotation
persistent identity: annotation2189489
display id: annotation2189489
version: 1
top level: CBDcex(J61
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K863104/annotation2189489/range2189489/1

https://synbiohub.org/public/igem/BBa_K863104/annotation2189489/range2189489/1
type: Range
persistent identity: range2189489
display id: range2189489
version: 1
direction: direction/forward
top level: CBDcex(J61
start: 83
end: 382
orientation: inline

TAA
type: SequenceAnnotation
persistent identity: annotation2189497
display id: annotation2189497
version: 1
top level: CBDcex(J61
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K863104/annotation2189497/range2189497/1

https://synbiohub.org/public/igem/BBa_K863104/annotation2189497/range2189497/1
type: Range
persistent identity: range2189497
display id: range2189497
version: 1
direction: direction/forward
top level: CBDcex(J61
start: 1142
end: 1144
orientation: inline

GFP2
type: ComponentDefinition
persistent identity: BBa I715020
display id: BBa_I715020
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Carboxyl Half of GFP (aka GFP2)
creator: Will DeLoache
created: 2007-06-21T11:00:00Z
modified: 2015-08-31T04:07:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _120_
m user id: 0
owner id: 1491
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: A
mutable notes: A
mutable provenance: A
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: Stop Codons (two in tandem)
sequence annotation: extra base to maintain reading frame
sequence annotation: Coding Carboxyl half of GFP (aka GFP2)
sequence: https://synbiohub.org/public/igem/BBa_I715020_sequence/1

Stop Codons (two in tandem)
type: SequenceAnnotation
persistent identity: annotation1935362
display id: annotation1935362
version: 1
top level: GFP2
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_I715020/annotation1935362/range1935362/1

https://synbiohub.org/public/igem/BBa_I715020/annotation1935362/range1935362/1
type: Range
persistent identity: range1935362
display id: range1935362
version: 1
direction: direction/forward
top level: GFP2
start: 245
end: 250
orientation: inline

extra base to maintain reading frame
type: SequenceAnnotation
persistent identity: annotation1935360
display id: annotation1935360
version: 1
top level: GFP2
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I715020/annotation1935360/range1935360/1

https://synbiohub.org/public/igem/BBa_I715020/annotation1935360/range1935360/1
type: Range
persistent identity: range1935360
display id: range1935360
version: 1
direction: direction/forward
top level: GFP2
start: 1
end: 1
orientation: inline

Coding Carboxyl half of GFP (aka GFP2)
type: SequenceAnnotation
persistent identity: annotation1935361
display id: annotation1935361
version: 1
top level: GFP2
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_I715020/annotation1935361/range1935361/1

https://synbiohub.org/public/igem/BBa_I715020/annotation1935361/range1935361/1
type: Range
persistent identity: range1935361
display id: range1935361
version: 1
direction: direction/forward
top level: GFP2
start: 1
end: 244
orientation: inline

BBa_K1679006
type: ComponentDefinition
persistent identity: BBa K1679006
display id: BBa_K1679006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RiboJ and GFP
creator: Qikai Qin
created: 2015-09-15T11:00:00Z
modified: 2015-09-18T08:44:26Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2097_
m user id: 21726
owner id: 21726
owning group id: 9
status: status/Planning
bookmark: true
mutable description: This part is a composite of RiboJ and I13504 which can be used to express GFP if added a promoter.
mutable notes: In order to make other team easily use a GFP part to test the strength of a promoter, we constructed this part contain RiboJ before the BBa_I13504. Any team can use this part to measure the GFP expression if added a promoter.
mutable provenance: RiboJ BBa_I13504
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_I13504
component: BBa_K1679038
sequence annotation: BBa_I13504
sequence annotation: BBa_K1679038
sequence: https://synbiohub.org/public/igem/BBa_K1679006_sequence/1

BBa_I13504
type: Component
persistent identity: component2474737
display id: component2474737
version: 1
top level: BBa_K1679006
access: public
definition: https://synbiohub.org/public/igem/BBa_I13504/1

BBa_K1679038
type: Component
persistent identity: component2474727
display id: component2474727
version: 1
top level: BBa_K1679006
access: public
definition: https://synbiohub.org/public/igem/BBa_K1679038/1

BBa_I13504
type: SequenceAnnotation
persistent identity: annotation2474737
display id: annotation2474737
version: 1
top level: BBa_K1679006
location: https://synbiohub.org/public/igem/BBa_K1679006/annotation2474737/range2474737/1
component: BBa_I13504

https://synbiohub.org/public/igem/BBa_K1679006/annotation2474737/range2474737/1
type: Range
persistent identity: range2474737
display id: range2474737
version: 1
direction: direction/reverse
top level: BBa_K1679006
start: 84
end: 958
orientation: inline

BBa_K1679038
type: SequenceAnnotation
persistent identity: annotation2474727
display id: annotation2474727
version: 1
top level: BBa_K1679006
location: https://synbiohub.org/public/igem/BBa_K1679006/annotation2474727/range2474727/1
component: BBa_K1679038

https://synbiohub.org/public/igem/BBa_K1679006/annotation2474727/range2474727/1
type: Range
persistent identity: range2474727
display id: range2474727
version: 1
direction: direction/reverse
top level: BBa_K1679006
start: 1
end: 75
orientation: inline

ECFP
type: ComponentDefinition
persistent identity: BBa E0022
display id: BBa_E0022
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: enhanced cyan fluorescent protein derived from A. victoria GFP
creator: June Rhee, Connie Tao, Ty Thomson, Louis Waldman
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:25Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Cyan fluorescent protein (ECFP) reporter coding sequence without the Ribosome Binding Site. Modified with an LVA tail for rapid degradation of the protein and faster fall time for the emission.
mutable notes: BBa_E0022 cyan fluorescent protein is based on BioBrick part BBa_E0021. It has been modified to include a rapid degradation LVA tail, and includes the BioBrick standard assembly head and tail restriction sites. The RBS has been removed. The stop codon has been changed from TAA to a double stop codon TAATAA.
mutable provenance: Modified from <bb_part>BBa_E0021</bb_part>.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: A
sequence annotation: 2
sequence annotation: CFP (LVA)
sequence annotation: BBa_E0022
sequence annotation: SsrA
sequence: https://synbiohub.org/public/igem/BBa_E0022_sequence/1

A
type: SequenceAnnotation
persistent identity: annotation2155
display id: annotation2155
version: 1
top level: ECFP
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_E0022/annotation2155/range2155/1

https://synbiohub.org/public/igem/BBa_E0022/annotation2155/range2155/1
type: Range
persistent identity: range2155
display id: range2155
version: 1
direction: direction/forward
top level: ECFP
start: 69
end: 69
orientation: inline

2
type: SequenceAnnotation
persistent identity: annotation2154
display id: annotation2154
version: 1
top level: ECFP
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_E0022/annotation2154/range2154/1

https://synbiohub.org/public/igem/BBa_E0022/annotation2154/range2154/1
type: Range
persistent identity: range2154
display id: range2154
version: 1
direction: direction/forward
top level: ECFP
start: 757
end: 762
orientation: inline

CFP (LVA)
type: SequenceAnnotation
persistent identity: annotation2150
display id: annotation2150
version: 1
top level: ECFP
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_E0022/annotation2150/range2150/1

https://synbiohub.org/public/igem/BBa_E0022/annotation2150/range2150/1
type: Range
persistent identity: range2150
display id: range2150
version: 1
direction: direction/forward
top level: ECFP
start: 1
end: 762
orientation: inline

BBa_E0022
type: SequenceAnnotation
persistent identity: annotation7040
display id: annotation7040
version: 1
top level: ECFP
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_E0022/annotation7040/range7040/1

https://synbiohub.org/public/igem/BBa_E0022/annotation7040/range7040/1
type: Range
persistent identity: range7040
display id: range7040
version: 1
direction: direction/forward
top level: ECFP
start: 1
end: 762
orientation: inline

SsrA
type: SequenceAnnotation
persistent identity: annotation2153
display id: annotation2153
version: 1
top level: ECFP
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_E0022/annotation2153/range2153/1

https://synbiohub.org/public/igem/BBa_E0022/annotation2153/range2153/1
type: Range
persistent identity: range2153
display id: range2153
version: 1
direction: direction/forward
top level: ECFP
start: 719
end: 756
orientation: inline

mRFP1
type: ComponentDefinition
persistent identity: BBa K678030
display id: BBa_K678030
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: mRFP1-module
creator: DTU-Denmark-2
created: 2011-09-08T11:00:00Z
modified: 2015-05-08T01:13:04Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _882_
m user id: 0
owner id: 8341
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Monomeric red fluorescent protein (mRFP1) is a derivative of the red fluorescent protein cloned from Discosoma coral (DsRed) (1). The successful use of mRFP1 in Aspergillus nidulans has previously been described (2).
mutable notes: This part was designed for the Plug'n'Play with DNA assembly standard (RFC 80), therefore this part contains tails at the 5' and 3' ends as shown on the figure below. Please notice that mRFP1-module is not the same as BBa_K678031 or BBa_K678032.
mutable provenance: From p71-mRFP1 of the Center for Microbial Biotechnology plasmid collection at the Technical University of Denmark
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: RFP
sequence annotation: Linker 2
sequence: https://synbiohub.org/public/igem/BBa_K678030_sequence/1

RFP
type: SequenceAnnotation
persistent identity: annotation2127389
display id: annotation2127389
version: 1
top level: mRFP1
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K678030/annotation2127389/range2127389/1

https://synbiohub.org/public/igem/BBa_K678030/annotation2127389/range2127389/1
type: Range
persistent identity: range2127389
display id: range2127389
version: 1
direction: direction/forward
top level: mRFP1
start: 9
end: 686
orientation: inline

Linker 2
type: SequenceAnnotation
persistent identity: annotation2127388
display id: annotation2127388
version: 1
top level: mRFP1
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678030/annotation2127388/range2127388/1

https://synbiohub.org/public/igem/BBa_K678030/annotation2127388/range2127388/1
type: Range
persistent identity: range2127388
display id: range2127388
version: 1
direction: direction/forward
top level: mRFP1
start: 1
end: 8
orientation: inline

BBa_K1486038
type: ComponentDefinition
persistent identity: BBa K1486038
display id: BBa_K1486038
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: sfGFPN
creator: EPFL iGem team 2014
created: 2014-09-26T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: To edit
mutable notes: To edit
mutable provenance: To edit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence: https://synbiohub.org/public/igem/BBa_K1486038_sequence/1

mRFP
type: ComponentDefinition
persistent identity: BBa K1615089
display id: BBa_K1615089
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: mRFP RFC25
creator: Brooke Rothschild-Mancinelli
created: 2015-08-30T11:00:00Z
modified: 2016-01-25T11:25:49Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2032_
m user id: 4206
owner id: 25547
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Highly engineered monomeric mutant of RFP. Improved version of part BBa_E1010 to remove illegal AgeI sites and add RFC25 prefix and suffix.
mutable notes: Codon optimised for E. coli. RFC 25 compatible.
mutable provenance: mRFP highly engineered variant originally from Discosoma striata.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: mRFP
sequence: https://synbiohub.org/public/igem/BBa_K1615089_sequence/1

mRFP
type: SequenceAnnotation
persistent identity: annotation2440569
display id: annotation2440569
version: 1
top level: mRFP
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1615089/annotation2440569/range2440569/1

https://synbiohub.org/public/igem/BBa_K1615089/annotation2440569/range2440569/1
type: Range
persistent identity: range2440569
display id: range2440569
version: 1
direction: direction/forward
top level: mRFP
start: 1
end: 675
orientation: inline

BBa_K533005
type: ComponentDefinition
persistent identity: BBa K533005
display id: BBa_K533005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: OmpA-GFP
creator: ZHANG Yunxiao
created: 2011-09-28T11:00:00Z
modified: 2015-05-08T01:12:36Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _699_
m user id: 0
owner id: 6884
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: GFP is presented outside E. Coli as a positive control for protein presentation
mutable notes: His tag is inserted between OmpA and GFP for easier detection in Western Blotting.
mutable provenance: OmpA is cloned from BL21(DE3) E. Coli strain. GFP is from Clontech plasmid pGFP-N1
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
sequence annotation: start
sequence annotation: lacI
sequence annotation: stop
sequence annotation: GFP
sequence annotation: His Tag
sequence annotation: OmpA
sequence annotation: rbs
sequence annotation: T7
sequence: https://synbiohub.org/public/igem/BBa_K533005_sequence/1

start
type: SequenceAnnotation
persistent identity: annotation2150604
display id: annotation2150604
version: 1
top level: BBa_K533005
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K533005/annotation2150604/range2150604/1

https://synbiohub.org/public/igem/BBa_K533005/annotation2150604/range2150604/1
type: Range
persistent identity: range2150604
display id: range2150604
version: 1
direction: direction/forward
top level: BBa_K533005
start: 87
end: 89
orientation: inline

lacI
type: SequenceAnnotation
persistent identity: annotation2150601
display id: annotation2150601
version: 1
top level: BBa_K533005
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K533005/annotation2150601/range2150601/1

https://synbiohub.org/public/igem/BBa_K533005/annotation2150601/range2150601/1
type: Range
persistent identity: range2150601
display id: range2150601
version: 1
direction: direction/forward
top level: BBa_K533005
start: 20
end: 44
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2150603
display id: annotation2150603
version: 1
top level: BBa_K533005
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K533005/annotation2150603/range2150603/1

https://synbiohub.org/public/igem/BBa_K533005/annotation2150603/range2150603/1
type: Range
persistent identity: range2150603
display id: range2150603
version: 1
direction: direction/forward
top level: BBa_K533005
start: 1416
end: 1418
orientation: inline

GFP
type: SequenceAnnotation
persistent identity: annotation2150598
display id: annotation2150598
version: 1
top level: BBa_K533005
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K533005/annotation2150598/range2150598/1

https://synbiohub.org/public/igem/BBa_K533005/annotation2150598/range2150598/1
type: Range
persistent identity: range2150598
display id: range2150598
version: 1
direction: direction/forward
top level: BBa_K533005
start: 696
end: 1418
orientation: inline

His Tag
type: SequenceAnnotation
persistent identity: annotation2150600
display id: annotation2150600
version: 1
top level: BBa_K533005
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K533005/annotation2150600/range2150600/1

https://synbiohub.org/public/igem/BBa_K533005/annotation2150600/range2150600/1
type: Range
persistent identity: range2150600
display id: range2150600
version: 1
direction: direction/forward
top level: BBa_K533005
start: 648
end: 665
orientation: inline

OmpA
type: SequenceAnnotation
persistent identity: annotation2150597
display id: annotation2150597
version: 1
top level: BBa_K533005
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K533005/annotation2150597/range2150597/1

https://synbiohub.org/public/igem/BBa_K533005/annotation2150597/range2150597/1
type: Range
persistent identity: range2150597
display id: range2150597
version: 1
direction: direction/forward
top level: BBa_K533005
start: 87
end: 633
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation2150599
display id: annotation2150599
version: 1
top level: BBa_K533005
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K533005/annotation2150599/range2150599/1

https://synbiohub.org/public/igem/BBa_K533005/annotation2150599/range2150599/1
type: Range
persistent identity: range2150599
display id: range2150599
version: 1
direction: direction/forward
top level: BBa_K533005
start: 75
end: 79
orientation: inline

T7
type: SequenceAnnotation
persistent identity: annotation2150602
display id: annotation2150602
version: 1
top level: BBa_K533005
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K533005/annotation2150602/range2150602/1

https://synbiohub.org/public/igem/BBa_K533005/annotation2150602/range2150602/1
type: Range
persistent identity: range2150602
display id: range2150602
version: 1
direction: direction/forward
top level: BBa_K533005
start: 1
end: 18
orientation: inline

BBa_K249014
type: ComponentDefinition
persistent identity: BBa K249014
display id: BBa_K249014
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: C-terminal YFP --> Tet inverter
creator: Roxanne Shank
created: 2009-08-25T11:00:00Z
modified: 2015-05-08T01:11:40Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _342_
m user id: 0
owner id: 3171
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Arabinose repressible Tetracycline inversion of yFP with N-terminal Fusion Arganine Tag for targetting into Lumazine Microcompartment. Has a medium RBS and a double terminator.
mutable notes: Standard
mutable provenance: BioBrick Registry and synthesized Arganine Tag
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_K249006
component: BBa_K249001
component: BBa_B0034
component: BBa_B0015
sequence annotation: BBa_K249006
sequence annotation: BBa_B0034
sequence annotation: BBa_K249001
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_K249014_sequence/1

BBa_K249006
type: Component
persistent identity: component2284690
display id: component2284690
version: 1
top level: BBa_K249014
access: public
definition: https://synbiohub.org/public/igem/BBa_K249006/1

BBa_K249001
type: Component
persistent identity: component2284687
display id: component2284687
version: 1
top level: BBa_K249014
access: public
definition: https://synbiohub.org/public/igem/BBa_K249001/1

BBa_B0034
type: Component
persistent identity: component2284689
display id: component2284689
version: 1
top level: BBa_K249014
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0015
type: Component
persistent identity: component2284697
display id: component2284697
version: 1
top level: BBa_K249014
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_K249006
type: SequenceAnnotation
persistent identity: annotation2284690
display id: annotation2284690
version: 1
top level: BBa_K249014
location: https://synbiohub.org/public/igem/BBa_K249014/annotation2284690/range2284690/1
component: BBa_K249006

https://synbiohub.org/public/igem/BBa_K249014/annotation2284690/range2284690/1
type: Range
persistent identity: range2284690
display id: range2284690
version: 1
direction: direction/reverse
top level: BBa_K249014
start: 1067
end: 1822
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2284689
display id: annotation2284689
version: 1
top level: BBa_K249014
location: https://synbiohub.org/public/igem/BBa_K249014/annotation2284689/range2284689/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K249014/annotation2284689/range2284689/1
type: Range
persistent identity: range2284689
display id: range2284689
version: 1
direction: direction/reverse
top level: BBa_K249014
start: 1049
end: 1060
orientation: inline

BBa_K249001
type: SequenceAnnotation
persistent identity: annotation2284687
display id: annotation2284687
version: 1
top level: BBa_K249014
location: https://synbiohub.org/public/igem/BBa_K249014/annotation2284687/range2284687/1
component: BBa_K249001

https://synbiohub.org/public/igem/BBa_K249014/annotation2284687/range2284687/1
type: Range
persistent identity: range2284687
display id: range2284687
version: 1
direction: direction/reverse
top level: BBa_K249014
start: 1
end: 1040
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2284697
display id: annotation2284697
version: 1
top level: BBa_K249014
location: https://synbiohub.org/public/igem/BBa_K249014/annotation2284697/range2284697/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K249014/annotation2284697/range2284697/1
type: Range
persistent identity: range2284697
display id: range2284697
version: 1
direction: direction/reverse
top level: BBa_K249014
start: 1831
end: 1959
orientation: inline

BBa_K1491004
type: ComponentDefinition
persistent identity: BBa K1491004
display id: BBa_K1491004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: SuperYFP2-His
creator: Danielle Peters
created: 2014-07-14T11:00:00Z
modified: 2016-01-21T02:28:40Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1871_
m user id: 4206
owner id: 22521
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: SuperYFP2 (BBa_K864100) with 6x His tag for protein purification.
mutable notes: YFP+His
mutable provenance: SuperYFP2 is BBa_K864100
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: 6x His
sequence annotation: BBa_K864100
sequence: https://synbiohub.org/public/igem/BBa_K1491004_sequence/1

6x His
type: SequenceAnnotation
persistent identity: annotation2380095
display id: annotation2380095
version: 1
top level: BBa_K1491004
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1491004/annotation2380095/range2380095/1

https://synbiohub.org/public/igem/BBa_K1491004/annotation2380095/range2380095/1
type: Range
persistent identity: range2380095
display id: range2380095
version: 1
direction: direction/forward
top level: BBa_K1491004
start: 718
end: 735
orientation: inline

BBa_K864100
type: SequenceAnnotation
persistent identity: annotation2380094
display id: annotation2380094
version: 1
top level: BBa_K1491004
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1491004/annotation2380094/range2380094/1

https://synbiohub.org/public/igem/BBa_K1491004/annotation2380094/range2380094/1
type: Range
persistent identity: range2380094
display id: range2380094
version: 1
direction: direction/forward
top level: BBa_K1491004
start: 1
end: 717
orientation: inline

mRFP1
type: ComponentDefinition
persistent identity: BBa K678031
display id: BBa_K678031
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: mRFP1-GOI
creator: DTU-Denmark-2
created: 2011-09-08T11:00:00Z
modified: 2015-05-08T01:13:04Z
discontinued: false
dominant: false
experience: experience/None
group u list: _882_
m user id: 0
owner id: 8341
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Monomeric red fluorescent protein (mRFP1) is a derivative of the red fluorescent protein cloned from Discosoma coral (DsRed) (1). The successful use of mRFP1 in Aspergillus nidulans has previously been described (2). This part does not contain a stop codon. {| border="1" !colspan="8"|Spectroscopic data (1) |- |Excitation maximum |Emission maximum |Extinction coefficient per chain |Fluorescence quantum yield |pKa |t0.5 for maturation at 37??C |colspan="2"|Rate of photobleach relative to EGFP |- |584 nm |607 nm |44,000 M-1cm-1 |0.25 |4.5 |<1h |fast 7.2 |slow 0.16 |}
mutable notes: This part was designed for the Plug'n'Play with DNA assembly standard (RFC 80), therefore this part contains tails at the 5' and 3' ends as shown on the figure below. Please notice that mRFP1-module is not the same as BBa_K678030 or BBa_K678032.
mutable provenance: From p71-mRFP1 of the Center for Microbial Biotechnology plasmid collection at the Technical University of Denmark
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: RFP
sequence annotation: Linker 2
sequence: https://synbiohub.org/public/igem/BBa_K678031_sequence/1

RFP
type: SequenceAnnotation
persistent identity: annotation2127391
display id: annotation2127391
version: 1
top level: mRFP1
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K678031/annotation2127391/range2127391/1

https://synbiohub.org/public/igem/BBa_K678031/annotation2127391/range2127391/1
type: Range
persistent identity: range2127391
display id: range2127391
version: 1
direction: direction/forward
top level: mRFP1
start: 9
end: 683
orientation: inline

Linker 2
type: SequenceAnnotation
persistent identity: annotation2127390
display id: annotation2127390
version: 1
top level: mRFP1
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678031/annotation2127390/range2127390/1

https://synbiohub.org/public/igem/BBa_K678031/annotation2127390/range2127390/1
type: Range
persistent identity: range2127390
display id: range2127390
version: 1
direction: direction/forward
top level: mRFP1
start: 1
end: 8
orientation: inline

BBa_J97000
type: ComponentDefinition
persistent identity: BBa J97000
display id: BBa_J97000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: IP-Free EiraCFP (Cyan Fluorescent Protein)
creator: Drew Endy
created: 2014-02-04T12:00:00Z
modified: 2015-05-08T01:08:32Z
discontinued: false
dominant: false
experience: experience/None
group u list: _41_1_
m user id: 0
owner id: 747
owning group id: 63
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This part was created by DNA 2.0 as part of their IP-Free series of fluorescent and chromogenic proteins. It is available to use under the BioBrick Public Agreement. Excitation 398nm, Emission 498nm Molecular Weight: 26.0kDA, Length: 233 amino acids
mutable notes: DNA 2.0 have created a large set of new colored and fluorescent proteins by back-translating a set of sequences from Genbank, using their proprietary GeneDesigner software. To create these proteins, they simultaneously minimized sequence differences and and derived an E. coli codon bias for high protein expression. Gene fragments were designed to create chimeric genes when pooled. A subset of the resultant chimeras were selected as base genes from which multiple iterations of libraries with targeted substitutions were made. (from DNA 2.0)
mutable provenance: Non-Aequorea based sequences.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence: https://synbiohub.org/public/igem/BBa_J97000_sequence/1

BBa_K1399000
type: ComponentDefinition
persistent identity: BBa K1399000
display id: BBa_K1399000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RFP from Discosoma striata (coral) with AAV-ssrA degradation tag
creator: Anna Stikane
created: 2014-09-16T11:00:00Z
modified: 2015-05-08T01:10:15Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1777_
m user id: 0
owner id: 22477
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: Mutant RFP from Discosoma striata (coral) (see part E1010) with added AAV-ssrA degradation tag (part I11012). The tag increases RFP turn-over rate, thus providing better temporal resolution of red fluorescence. In the same time, maximal fluorescence amplitudes will be lower as newly formed protein is degraded as soon as it is formed. The tag encodes peptide sequence AANDENYAAAV and is recognized by ClpA and ClpX unfoldases and ClpX mediator SspB.[1] ClpA and ClpX then form a proteosome-like complex with ClpP protease and the protein is degraded.[1] The final three residues of the tag determines the strength of interaction with ClpX and thus the final protein degradation rate.[2] The AAV tag is reported to lead to reasonably fast protein degradation, degrading GFP with rate -0.012 per minute.[2] However, be aware that exact protein degradation rate depends on multiple factors: 1) ClpXP and ClpAP protease and SspB mediator concentrations; 2) Protein stability; 3) Km of binding to the protease; 4) Temperature [3]. [1] Flynn, J. M. et al. Overlapping recognition determinants within the ssrA degradation tag allow modulation of proteolysis. Proc. Natl. Acad. Sci. U. S. A. 98, 10584???9 (2001). [2] Andersen, J. B. et al. New unstable variants of green fluorescent protein for studies of transient gene expression in bacteria. Appl. Environ. Microbiol. 64, 2240???6 (1998). [3]Purcell, O., Grierson, C. S., Bernardo, M. Di & Savery, N. J. Temperature dependence of ssrA-tag mediated protein degradation. J. Biol. Eng. 6, 10 (2012).
mutable notes: The tag was attached to RFP using PCR and MABEL (mutagenesis with blunt-end ligation), thus avoiding introduction of additonal residues and restriction site. Different parts of the tag are recognized by different proteins, for example, the final 3 residues (AAV in this case) are recognised by ClpX, whereas first 4 residues of the tag are required for efficient SspB binding.[1] Thus modifications of these critical residues alter the efficacy with what different proteases bind to it.
mutable provenance: RFP comes from part E1010, tag sequence was obtained from part I11012, but same tag was also usead in paper by Andersen et al., (1998).[2]
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: stop
sequence annotation: AAV-ssrA tag
sequence annotation: cds
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_K1399000_sequence/1

stop
type: SequenceAnnotation
persistent identity: annotation2383858
display id: annotation2383858
version: 1
top level: BBa_K1399000
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1399000/annotation2383858/range2383858/1

https://synbiohub.org/public/igem/BBa_K1399000/annotation2383858/range2383858/1
type: Range
persistent identity: range2383858
display id: range2383858
version: 1
direction: direction/forward
top level: BBa_K1399000
start: 712
end: 714
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2383857
display id: annotation2383857
version: 1
top level: BBa_K1399000
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1399000/annotation2383857/range2383857/1

https://synbiohub.org/public/igem/BBa_K1399000/annotation2383857/range2383857/1
type: Range
persistent identity: range2383857
display id: range2383857
version: 1
direction: direction/forward
top level: BBa_K1399000
start: 709
end: 711
orientation: inline

AAV-ssrA tag
type: SequenceAnnotation
persistent identity: annotation2383856
display id: annotation2383856
version: 1
top level: BBa_K1399000
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1399000/annotation2383856/range2383856/1

https://synbiohub.org/public/igem/BBa_K1399000/annotation2383856/range2383856/1
type: Range
persistent identity: range2383856
display id: range2383856
version: 1
direction: direction/forward
top level: BBa_K1399000
start: 676
end: 708
orientation: inline

cds
type: SequenceAnnotation
persistent identity: annotation2383855
display id: annotation2383855
version: 1
top level: BBa_K1399000
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1399000/annotation2383855/range2383855/1

https://synbiohub.org/public/igem/BBa_K1399000/annotation2383855/range2383855/1
type: Range
persistent identity: range2383855
display id: range2383855
version: 1
direction: direction/forward
top level: BBa_K1399000
start: 4
end: 675
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2383854
display id: annotation2383854
version: 1
top level: BBa_K1399000
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1399000/annotation2383854/range2383854/1

https://synbiohub.org/public/igem/BBa_K1399000/annotation2383854/range2383854/1
type: Range
persistent identity: range2383854
display id: range2383854
version: 1
direction: direction/forward
top level: BBa_K1399000
start: 1
end: 3
orientation: inline

BBa_K1833001
type: ComponentDefinition
persistent identity: BBa K1833001
display id: BBa_K1833001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pProt-GFP
creator: Konstantin Borisov
created: 2015-09-15T11:00:00Z
modified: 2015-09-16T08:02:07Z
discontinued: false
dominant: false
group u list: _2259_
m user id: 27388
owner id: 27388
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This part produces GFP in the presence of sigma-70 E. coli RNA polymerase; however, the promoter contains two sites for a synthetic repressor to bind. Without the presence of this repressor, the part can serve as a strong constitutive promoter, as shown in the pictures below. For more information on the repressor, visit http://2015.igem.org/Team:Pitt/Protease/Project. The GFP produced is a mutant known as GFPmut3b, and the original citation is as follows: Cormack, B.P., Valdivia, R.H., and S. Falkow. FACS-optimized mutants of green fluorescent protein (GFP). Gene 173: 33-38 (1996). (http://www.sciencedirect.com/science/article/pii/0378111995006850) It has a maximum excitation at 501 nm and maximum emission at 511 nm. For more information, see part BBa_E0040.
mutable notes: The part containing GFP was cut with EcoRI and XbaI, and ligated with the annealed oligos to form the completed part. Note that due to the choice of technique, a Biobrick scar site remains between the promoter and RBS.
mutable provenance: The RBS-GFP-terminator was obtained from as part BBa_E0840 from the 2015 Distribution (Plate 2, Well 24D). The pProt promoter was obtained as two short oligonucleotides annealed to form sticky ends. The part containing GFP was then cut with EcoRI and XbaI, and ligated with the annealed oligos to form the completed part.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_E0840
component: BBa_K1833998
sequence annotation: BBa_E0840
sequence annotation: BBa_K1833998
sequence: https://synbiohub.org/public/igem/BBa_K1833001_sequence/1

BBa_E0840
type: Component
persistent identity: component2460170
display id: component2460170
version: 1
top level: BBa_K1833001
access: public
definition: https://synbiohub.org/public/igem/BBa_E0840/1

BBa_K1833998
type: Component
persistent identity: component2460159
display id: component2460159
version: 1
top level: BBa_K1833001
access: public
definition: https://synbiohub.org/public/igem/BBa_K1833998/1

BBa_E0840
type: SequenceAnnotation
persistent identity: annotation2460170
display id: annotation2460170
version: 1
top level: BBa_K1833001
location: https://synbiohub.org/public/igem/BBa_K1833001/annotation2460170/range2460170/1
component: BBa_E0840

https://synbiohub.org/public/igem/BBa_K1833001/annotation2460170/range2460170/1
type: Range
persistent identity: range2460170
display id: range2460170
version: 1
direction: direction/reverse
top level: BBa_K1833001
start: 54
end: 931
orientation: inline

BBa_K1833998
type: SequenceAnnotation
persistent identity: annotation2460159
display id: annotation2460159
version: 1
top level: BBa_K1833001
location: https://synbiohub.org/public/igem/BBa_K1833001/annotation2460159/range2460159/1
component: BBa_K1833998

https://synbiohub.org/public/igem/BBa_K1833001/annotation2460159/range2460159/1
type: Range
persistent identity: range2460159
display id: range2460159
version: 1
direction: direction/reverse
top level: BBa_K1833001
start: 1
end: 53
orientation: inline

BBa_K621000
type: ComponentDefinition
persistent identity: BBa K621000
display id: BBa_K621000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS-3 -> EYFP
creator: David Pohlman, Blythe Ferriere, Aile Able, Erica McFarland
created: 2011-09-26T11:00:00Z
modified: 2015-05-08T01:12:55Z
discontinued: false
dominant: false
experience: experience/None
group u list: _793_
m user id: 0
owner id: 10647
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: The RBS-3, BBa_B0032, is a medium-strength ribosome bonding site. EYFP, BBa_E0030, is a constituent of GFP, green fluorescence protien. With these two pieces together, this part allows for translation of the EYFP gene.
mutable notes: N/A
mutable provenance: The two individual parts, BBa_B0032 (RBS-3) and BBa_E0030 (EYFP), came from the iGEM Registry and were subcloned together by our team.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_E0030
component: BBa_B0032
sequence annotation: BBa_E0030
sequence annotation: BBa_B0032
sequence: https://synbiohub.org/public/igem/BBa_K621000_sequence/1

BBa_E0030
type: Component
persistent identity: component2144391
display id: component2144391
version: 1
top level: BBa_K621000
access: public
definition: eyfp

BBa_B0032
type: Component
persistent identity: component2144389
display id: component2144389
version: 1
top level: BBa_K621000
access: public
definition: https://synbiohub.org/public/igem/BBa_B0032/1

BBa_E0030
type: SequenceAnnotation
persistent identity: annotation2144391
display id: annotation2144391
version: 1
top level: BBa_K621000
location: https://synbiohub.org/public/igem/BBa_K621000/annotation2144391/range2144391/1
component: BBa_E0030

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type: Range
persistent identity: range2144391
display id: range2144391
version: 1
direction: direction/reverse
top level: BBa_K621000
start: 20
end: 742
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation2144389
display id: annotation2144389
version: 1
top level: BBa_K621000
location: https://synbiohub.org/public/igem/BBa_K621000/annotation2144389/range2144389/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_K621000/annotation2144389/range2144389/1
type: Range
persistent identity: range2144389
display id: range2144389
version: 1
direction: direction/forward
top level: BBa_K621000
start: 1
end: 13
orientation: inline

BBa_K180019
type: ComponentDefinition
persistent identity: BBa K180019
display id: BBa_K180019
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Scissors - Primary plasmid (part 2) [tac pR, mBanana and LuxI generator]
creator: Richard Wilson, Stephanie Chambers
created: 2009-10-18T11:00:00Z
modified: 2015-05-08T01:11:06Z
discontinued: false
dominant: false
experience: experience/None
group u list: _278_
m user id: 0
owner id: 4082
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Part of the iGEM2009 Team:Southampton rock-paper-scissors 'scissors' primary plasmid.
mutable notes: Regulated expression of LasI (C0178) and mBanana (K180009) by tac pR (K180000), controlled by presence of lac/IPTG or trp in the cell.
mutable provenance: See relevant basic biobrick parts (K180000, B0034, K180009, B0015).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K180000
component: BBa_K180009
component: BBa_B0034
component: BBa_B0010
component: BBa_B0012
component: BBa_C0161
sequence annotation: BBa_B0010
sequence annotation: BBa_K180000
sequence annotation: BBa_B0012
sequence annotation: BBa_C0161
sequence annotation: BBa_K180009
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K180019_sequence/1

BBa_K180000
type: Component
persistent identity: component2058401
display id: component2058401
version: 1
top level: BBa_K180019
access: public
definition: https://synbiohub.org/public/igem/BBa_K180000/1

BBa_K180009
type: Component
persistent identity: component2058405
display id: component2058405
version: 1
top level: BBa_K180019
access: public
definition: BBa_K180009

BBa_B0034
type: Component
persistent identity: component2058403
display id: component2058403
version: 1
top level: BBa_K180019
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0010
type: Component
persistent identity: component2058410
display id: component2058410
version: 1
top level: BBa_K180019
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
type: Component
persistent identity: component2058412
display id: component2058412
version: 1
top level: BBa_K180019
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_C0161
type: Component
persistent identity: component2058409
display id: component2058409
version: 1
top level: BBa_K180019
access: public
definition: https://synbiohub.org/public/igem/BBa_C0161/1

BBa_B0034
type: Component
persistent identity: component2058407
display id: component2058407
version: 1
top level: BBa_K180019
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2058410
display id: annotation2058410
version: 1
top level: BBa_K180019
location: https://synbiohub.org/public/igem/BBa_K180019/annotation2058410/range2058410/1
component: BBa_B0010

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type: Range
persistent identity: range2058410
display id: range2058410
version: 1
direction: direction/forward
top level: BBa_K180019
start: 2801
end: 2880
orientation: inline

BBa_K180000
type: SequenceAnnotation
persistent identity: annotation2058401
display id: annotation2058401
version: 1
top level: BBa_K180019
location: https://synbiohub.org/public/igem/BBa_K180019/annotation2058401/range2058401/1
component: BBa_K180000

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type: Range
persistent identity: range2058401
display id: range2058401
version: 1
direction: direction/reverse
top level: BBa_K180019
start: 1
end: 1447
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2058412
display id: annotation2058412
version: 1
top level: BBa_K180019
location: https://synbiohub.org/public/igem/BBa_K180019/annotation2058412/range2058412/1
component: BBa_B0012

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type: Range
persistent identity: range2058412
display id: range2058412
version: 1
direction: direction/forward
top level: BBa_K180019
start: 2889
end: 2929
orientation: inline

BBa_C0161
type: SequenceAnnotation
persistent identity: annotation2058409
display id: annotation2058409
version: 1
top level: BBa_K180019
location: https://synbiohub.org/public/igem/BBa_K180019/annotation2058409/range2058409/1
component: BBa_C0161

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type: Range
persistent identity: range2058409
display id: range2058409
version: 1
direction: direction/reverse
top level: BBa_K180019
start: 2208
end: 2792
orientation: inline

BBa_K180009
type: SequenceAnnotation
persistent identity: annotation2058405
display id: annotation2058405
version: 1
top level: BBa_K180019
location: https://synbiohub.org/public/igem/BBa_K180019/annotation2058405/range2058405/1
component: BBa_K180009

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type: Range
persistent identity: range2058405
display id: range2058405
version: 1
direction: direction/reverse
top level: BBa_K180019
start: 1474
end: 2181
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2058407
display id: annotation2058407
version: 1
top level: BBa_K180019
location: https://synbiohub.org/public/igem/BBa_K180019/annotation2058407/range2058407/1
component: BBa_B0034

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type: Range
persistent identity: range2058407
display id: range2058407
version: 1
direction: direction/reverse
top level: BBa_K180019
start: 2190
end: 2201
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2058403
display id: annotation2058403
version: 1
top level: BBa_K180019
location: https://synbiohub.org/public/igem/BBa_K180019/annotation2058403/range2058403/1
component: BBa_B0034

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type: Range
persistent identity: range2058403
display id: range2058403
version: 1
direction: direction/reverse
top level: BBa_K180019
start: 1456
end: 1467
orientation: inline

BBa_K1742003
type: ComponentDefinition
persistent identity: BBa K1742003
display id: BBa_K1742003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Dronpa 145K
creator: Iv??n Casas Rodrigo
created: 2015-09-03T11:00:00Z
modified: 2016-01-25T11:27:33Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2164_
m user id: 4206
owner id: 26598
owning group id: 9
status: status/Planning
bookmark: false
mutable description: From Pectinidae sp. It is a CDS of green fluorescent protein Dronpa.
mutable notes: It is codon optimized for plants.
mutable provenance: Pectinidae sp. sequence found in the following article Lummer, M., Humpert, F., Steuwe, C., Caesar, K., Sch??ttpelz, M., Sauer, M. and Staiger, D. (2011), Reversible Photoswitchable DRONPA-s Monitors Nucleocytoplasmic Transport of an RNA-Binding Protein in Transgenic Plants. Traffic, 12: 693???702. doi: 10.1111/j.1600-0854.2011.01180.x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1742003_sequence/1

BBa_K1486009
type: ComponentDefinition
persistent identity: BBa K1486009
display id: BBa_K1486009
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CxpR & Split IFP1.4 [Nterm + Nterm]
creator: iGem EPFL 2014
created: 2014-08-26T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part has two fused proteins downstream of an arabinose-inducible promoter: CpxR-IFP[1] and CpxR-IFP[2]. This construct was made in all orientations: - CpxR-IFP[1] + CpxR-IFP[2] (BBa_K1286008) - IFP[1]-CpxR + CpxR-IFP[2] (BBa_K1486010) - CpxR-IFP[1] + IFP[2]-CpxR (BBa_K1486011)
mutable notes: All orientations were made, so all dimerizations could be tested (see main page for biobrick numbers). Flexible linkers were used for each of the fused proteins, and an RBS was put before each one of them.
mutable provenance: From Michnick Article. To Edit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K1486007
component: BBa_I0500
component: BBa_K1486004
component: BBa_B0034
component: BBa_K1486000
component: BBa_K1486052
component: BBa_K1486006
sequence annotation: BBa_K1486000
sequence annotation: BBa_K1486007
sequence annotation: BBa_B0034
sequence annotation: BBa_K1486006
sequence annotation: BBa_I0500
sequence annotation: BBa_K1486004
sequence annotation: BBa_K1486052
sequence: https://synbiohub.org/public/igem/BBa_K1486009_sequence/1

BBa_K1486007
type: Component
persistent identity: component2420783
display id: component2420783
version: 1
top level: BBa_K1486009
access: public
definition: BBa_K1486007

BBa_I0500
type: Component
persistent identity: component2420763
display id: component2420763
version: 1
top level: BBa_K1486009
access: public
definition: https://synbiohub.org/public/igem/BBa_I0500/1

BBa_K1486004
type: Component
persistent identity: component2420758
display id: component2420758
version: 1
top level: BBa_K1486009
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486004/1

BBa_B0034
type: Component
persistent identity: component2420767
display id: component2420767
version: 1
top level: BBa_K1486009
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K1486000
type: Component
persistent identity: component2420766
display id: component2420766
version: 1
top level: BBa_K1486009
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486000/1

BBa_K1486007
type: Component
persistent identity: component2420756
display id: component2420756
version: 1
top level: BBa_K1486009
access: public
definition: BBa_K1486007

BBa_K1486052
type: Component
persistent identity: component2420777
display id: component2420777
version: 1
top level: BBa_K1486009
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486052/1

BBa_B0034
type: Component
persistent identity: component2420779
display id: component2420779
version: 1
top level: BBa_K1486009
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K1486000
type: Component
persistent identity: component2420776
display id: component2420776
version: 1
top level: BBa_K1486009
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486000/1

BBa_K1486006
type: Component
persistent identity: component2420771
display id: component2420771
version: 1
top level: BBa_K1486009
access: public
definition: BBa_K1486006

BBa_K1486000
type: Component
persistent identity: component2420788
display id: component2420788
version: 1
top level: BBa_K1486009
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486000/1

BBa_K1486004
type: Component
persistent identity: component2420784
display id: component2420784
version: 1
top level: BBa_K1486009
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486004/1

BBa_K1486004
type: Component
persistent identity: component2420772
display id: component2420772
version: 1
top level: BBa_K1486009
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486004/1

BBa_K1486000
type: SequenceAnnotation
persistent identity: annotation2420766
display id: annotation2420766
version: 1
top level: BBa_K1486009
location: https://synbiohub.org/public/igem/BBa_K1486009/annotation2420766/range2420766/1
component: BBa_K1486000

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type: Range
persistent identity: range2420766
display id: range2420766
version: 1
direction: direction/reverse
top level: BBa_K1486009
start: 3025
end: 3726
orientation: inline

BBa_K1486007
type: SequenceAnnotation
persistent identity: annotation2420783
display id: annotation2420783
version: 1
top level: BBa_K1486009
location: https://synbiohub.org/public/igem/BBa_K1486009/annotation2420783/range2420783/1
component: BBa_K1486007

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type: Range
persistent identity: range2420783
display id: range2420783
version: 1
direction: direction/reverse
top level: BBa_K1486009
start: 2410
end: 2994
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2420767
display id: annotation2420767
version: 1
top level: BBa_K1486009
location: https://synbiohub.org/public/igem/BBa_K1486009/annotation2420767/range2420767/1
component: BBa_B0034

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type: Range
persistent identity: range2420767
display id: range2420767
version: 1
direction: direction/reverse
top level: BBa_K1486009
start: 1211
end: 1222
orientation: inline

BBa_K1486006
type: SequenceAnnotation
persistent identity: annotation2420771
display id: annotation2420771
version: 1
top level: BBa_K1486009
location: https://synbiohub.org/public/igem/BBa_K1486009/annotation2420771/range2420771/1
component: BBa_K1486006

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persistent identity: range2420771
display id: range2420771
version: 1
direction: direction/reverse
top level: BBa_K1486009
start: 1223
end: 1627
orientation: inline

BBa_K1486007
type: SequenceAnnotation
persistent identity: annotation2420756
display id: annotation2420756
version: 1
top level: BBa_K1486009
location: https://synbiohub.org/public/igem/BBa_K1486009/annotation2420756/range2420756/1
component: BBa_K1486007

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type: Range
persistent identity: range2420756
display id: range2420756
version: 1
direction: direction/reverse
top level: BBa_K1486009
start: 2410
end: 2994
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BBa_I0500
type: SequenceAnnotation
persistent identity: annotation2420763
display id: annotation2420763
version: 1
top level: BBa_K1486009
location: https://synbiohub.org/public/igem/BBa_K1486009/annotation2420763/range2420763/1
component: BBa_I0500

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type: Range
persistent identity: range2420763
display id: range2420763
version: 1
direction: direction/reverse
top level: BBa_K1486009
start: 1
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orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2420779
display id: annotation2420779
version: 1
top level: BBa_K1486009
location: https://synbiohub.org/public/igem/BBa_K1486009/annotation2420779/range2420779/1
component: BBa_B0034

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persistent identity: range2420779
display id: range2420779
version: 1
direction: direction/reverse
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BBa_K1486004
type: SequenceAnnotation
persistent identity: annotation2420758
display id: annotation2420758
version: 1
top level: BBa_K1486009
location: https://synbiohub.org/public/igem/BBa_K1486009/annotation2420758/range2420758/1
component: BBa_K1486004

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persistent identity: range2420758
display id: range2420758
version: 1
direction: direction/reverse
top level: BBa_K1486009
start: 2995
end: 3024
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BBa_K1486004
type: SequenceAnnotation
persistent identity: annotation2420772
display id: annotation2420772
version: 1
top level: BBa_K1486009
location: https://synbiohub.org/public/igem/BBa_K1486009/annotation2420772/range2420772/1
component: BBa_K1486004

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type: Range
persistent identity: range2420772
display id: range2420772
version: 1
direction: direction/reverse
top level: BBa_K1486009
start: 1628
end: 1657
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BBa_K1486000
type: SequenceAnnotation
persistent identity: annotation2420776
display id: annotation2420776
version: 1
top level: BBa_K1486009
location: https://synbiohub.org/public/igem/BBa_K1486009/annotation2420776/range2420776/1
component: BBa_K1486000

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type: Range
persistent identity: range2420776
display id: range2420776
version: 1
direction: direction/reverse
top level: BBa_K1486009
start: 1658
end: 2359
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BBa_K1486052
type: SequenceAnnotation
persistent identity: annotation2420777
display id: annotation2420777
version: 1
top level: BBa_K1486009
location: https://synbiohub.org/public/igem/BBa_K1486009/annotation2420777/range2420777/1
component: BBa_K1486052

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type: Range
persistent identity: range2420777
display id: range2420777
version: 1
direction: direction/reverse
top level: BBa_K1486009
start: 2360
end: 2397
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BBa_K1486000
type: SequenceAnnotation
persistent identity: annotation2420788
display id: annotation2420788
version: 1
top level: BBa_K1486009
location: https://synbiohub.org/public/igem/BBa_K1486009/annotation2420788/range2420788/1
component: BBa_K1486000

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type: Range
persistent identity: range2420788
display id: range2420788
version: 1
direction: direction/reverse
top level: BBa_K1486009
start: 3025
end: 3726
orientation: inline

BBa_K1486004
type: SequenceAnnotation
persistent identity: annotation2420784
display id: annotation2420784
version: 1
top level: BBa_K1486009
location: https://synbiohub.org/public/igem/BBa_K1486009/annotation2420784/range2420784/1
component: BBa_K1486004

https://synbiohub.org/public/igem/BBa_K1486009/annotation2420784/range2420784/1
type: Range
persistent identity: range2420784
display id: range2420784
version: 1
direction: direction/reverse
top level: BBa_K1486009
start: 2995
end: 3024
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BBa_K1680018
type: ComponentDefinition
persistent identity: BBa K1680018
display id: BBa_K1680018
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Cre-Dronpa fusion
creator: Nicolai von K??gelgen
created: 2015-09-16T11:00:00Z
modified: 2016-01-26T10:15:36Z
discontinued: false
dominant: false
group u list: _2098_
m user id: 4206
owner id: 26366
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Protein coding region for a fusion construct of Cre Recombinase and 145N Dronpa with 9 amino acid linker. Oligomerisation of Dronpa should stop Cre from accessing DNA in the nucleus thereby inhibiting it until light deactivation of dronpa.
mutable notes: Assembled using 3A assembly and RFC25.
mutable provenance: gBlocks designed by Team Tuebingen
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: K1680006
sequence annotation: K1680001
sequence annotation: K1680007
sequence: https://synbiohub.org/public/igem/BBa_K1680018_sequence/1

K1680006
type: SequenceAnnotation
persistent identity: annotation2477354
display id: annotation2477354
version: 1
top level: BBa_K1680018
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1680018/annotation2477354/range2477354/1

https://synbiohub.org/public/igem/BBa_K1680018/annotation2477354/range2477354/1
type: Range
persistent identity: range2477354
display id: range2477354
version: 1
direction: direction/forward
top level: BBa_K1680018
start: 1078
end: 1746
orientation: inline

K1680001
type: SequenceAnnotation
persistent identity: annotation2477357
display id: annotation2477357
version: 1
top level: BBa_K1680018
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1680018/annotation2477357/range2477357/1

https://synbiohub.org/public/igem/BBa_K1680018/annotation2477357/range2477357/1
type: Range
persistent identity: range2477357
display id: range2477357
version: 1
direction: direction/forward
top level: BBa_K1680018
start: 1
end: 21
orientation: inline

K1680001
type: SequenceAnnotation
persistent identity: annotation2477358
display id: annotation2477358
version: 1
top level: BBa_K1680018
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1680018/annotation2477358/range2477358/1

https://synbiohub.org/public/igem/BBa_K1680018/annotation2477358/range2477358/1
type: Range
persistent identity: range2477358
display id: range2477358
version: 1
direction: direction/forward
top level: BBa_K1680018
start: 1051
end: 1077
orientation: inline

K1680007
type: SequenceAnnotation
persistent identity: annotation2477355
display id: annotation2477355
version: 1
top level: BBa_K1680018
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1680018/annotation2477355/range2477355/1

https://synbiohub.org/public/igem/BBa_K1680018/annotation2477355/range2477355/1
type: Range
persistent identity: range2477355
display id: range2477355
version: 1
direction: direction/forward
top level: BBa_K1680018
start: 22
end: 1050
orientation: inline

BBa_K1321337
type: ComponentDefinition
persistent identity: BBa K1321337
display id: BBa_K1321337
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: sfGFP in Freiburg format (RFC 25)
creator: Chris N Micklem
created: 2014-10-07T11:00:00Z
modified: 2016-01-26T02:10:16Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1696_
m user id: 4206
owner id: 20830
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: sfGFP in Freiburg format (RFC 25) to allow for easy use in fusion proteins.
mutable notes: OEPCR
mutable provenance: Made from existing part.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: sfGFP
sequence: https://synbiohub.org/public/igem/BBa_K1321337_sequence/1

sfGFP
type: SequenceAnnotation
persistent identity: annotation2417826
display id: annotation2417826
version: 1
top level: BBa_K1321337
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1321337/annotation2417826/range2417826/1

https://synbiohub.org/public/igem/BBa_K1321337/annotation2417826/range2417826/1
type: Range
persistent identity: range2417826
display id: range2417826
version: 1
direction: direction/forward
top level: BBa_K1321337
start: 1
end: 711
orientation: inline

BBa_K1418040
type: ComponentDefinition
persistent identity: BBa K1418040
display id: BBa_K1418040
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CFP
creator: Cody Maxfield
created: 2014-10-07T11:00:00Z
modified: 2016-01-26T02:10:52Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1796_
m user id: 4206
owner id: 13965
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: CFP
mutable notes: RFC 23
mutable provenance: CFP
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: atg
sequence annotation: CFP
sequence annotation: taataa
sequence: https://synbiohub.org/public/igem/BBa_K1418040_sequence/1

atg
type: SequenceAnnotation
persistent identity: annotation2421328
display id: annotation2421328
version: 1
top level: BBa_K1418040
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1418040/annotation2421328/range2421328/1

https://synbiohub.org/public/igem/BBa_K1418040/annotation2421328/range2421328/1
type: Range
persistent identity: range2421328
display id: range2421328
version: 1
direction: direction/forward
top level: BBa_K1418040
start: 1
end: 3
orientation: inline

CFP
type: SequenceAnnotation
persistent identity: annotation2421329
display id: annotation2421329
version: 1
top level: BBa_K1418040
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1418040/annotation2421329/range2421329/1

https://synbiohub.org/public/igem/BBa_K1418040/annotation2421329/range2421329/1
type: Range
persistent identity: range2421329
display id: range2421329
version: 1
direction: direction/forward
top level: BBa_K1418040
start: 4
end: 717
orientation: inline

taataa
type: SequenceAnnotation
persistent identity: annotation2421352
display id: annotation2421352
version: 1
top level: BBa_K1418040
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1418040/annotation2421352/range2421352/1

https://synbiohub.org/public/igem/BBa_K1418040/annotation2421352/range2421352/1
type: Range
persistent identity: range2421352
display id: range2421352
version: 1
direction: direction/forward
top level: BBa_K1418040
start: 718
end: 723
orientation: inline

BBa_K332022
type: ComponentDefinition
persistent identity: BBa K332022
display id: BBa_K332022
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Plux + RBS + mRFP + double terminator
creator: Chin-Han Huang
created: 2010-10-23T11:00:00Z
modified: 2015-05-08T01:12:05Z
discontinued: false
dominant: false
experience: experience/None
group u list: _452_
m user id: 0
owner id: 7483
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: With Plux gene,it can detect the concentration of AHL-LuxR protein complex and initiate the following gene Sequence and Features
mutable notes: to detect cell's population
mutable provenance: all from IGEM's gene bank
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_E1010
component: BBa_R0062
component: BBa_J61048
component: BBa_B0034
sequence annotation: BBa_R0062
sequence annotation: BBa_B0034
sequence annotation: BBa_J61048
sequence annotation: BBa_E1010
sequence: https://synbiohub.org/public/igem/BBa_K332022_sequence/1

BBa_E1010
type: Component
persistent identity: component2250418
display id: component2250418
version: 1
top level: BBa_K332022
access: public
definition: mRFP1

BBa_R0062
type: Component
persistent identity: component2250409
display id: component2250409
version: 1
top level: BBa_K332022
access: public
definition: https://synbiohub.org/public/igem/BBa_R0062/1

BBa_J61048
type: Component
persistent identity: component2250419
display id: component2250419
version: 1
top level: BBa_K332022
access: public
definition: https://synbiohub.org/public/igem/BBa_J61048/1

BBa_B0034
type: Component
persistent identity: component2250415
display id: component2250415
version: 1
top level: BBa_K332022
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_R0062
type: SequenceAnnotation
persistent identity: annotation2250409
display id: annotation2250409
version: 1
top level: BBa_K332022
location: https://synbiohub.org/public/igem/BBa_K332022/annotation2250409/range2250409/1
component: BBa_R0062

https://synbiohub.org/public/igem/BBa_K332022/annotation2250409/range2250409/1
type: Range
persistent identity: range2250409
display id: range2250409
version: 1
direction: direction/forward
top level: BBa_K332022
start: 1
end: 55
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2250415
display id: annotation2250415
version: 1
top level: BBa_K332022
location: https://synbiohub.org/public/igem/BBa_K332022/annotation2250415/range2250415/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K332022/annotation2250415/range2250415/1
type: Range
persistent identity: range2250415
display id: range2250415
version: 1
direction: direction/reverse
top level: BBa_K332022
start: 64
end: 75
orientation: inline

BBa_J61048
type: SequenceAnnotation
persistent identity: annotation2250419
display id: annotation2250419
version: 1
top level: BBa_K332022
location: https://synbiohub.org/public/igem/BBa_K332022/annotation2250419/range2250419/1
component: BBa_J61048

https://synbiohub.org/public/igem/BBa_K332022/annotation2250419/range2250419/1
type: Range
persistent identity: range2250419
display id: range2250419
version: 1
direction: direction/reverse
top level: BBa_K332022
start: 796
end: 908
orientation: inline

BBa_E1010
type: SequenceAnnotation
persistent identity: annotation2250418
display id: annotation2250418
version: 1
top level: BBa_K332022
location: https://synbiohub.org/public/igem/BBa_K332022/annotation2250418/range2250418/1
component: BBa_E1010

https://synbiohub.org/public/igem/BBa_K332022/annotation2250418/range2250418/1
type: Range
persistent identity: range2250418
display id: range2250418
version: 1
direction: direction/reverse
top level: BBa_K332022
start: 82
end: 787
orientation: inline

ecfp
type: ComponentDefinition
persistent identity: BBa I15019
display id: BBa_I15019
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: B0031.ECFP
creator: Jeff Tabor
created: 2005-04-14T11:00:00Z
modified: 2015-08-31T04:07:39Z
discontinued: false
dominant: false
experience: experience/None
group u list: _5_
m user id: 0
owner id: 88
owning group id: 5
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part accepts POPS to produce ECFP under the weak RBS B0031
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_B0031
component: BBa_E0020
sequence annotation: BBa_E0020
sequence annotation: BBa_B0031
sequence: https://synbiohub.org/public/igem/BBa_I15019_sequence/1

BBa_B0031
type: Component
persistent identity: component1478455
display id: component1478455
version: 1
top level: ecfp
access: public
definition: https://synbiohub.org/public/igem/BBa_B0031/1

BBa_E0020
type: Component
persistent identity: component1478457
display id: component1478457
version: 1
top level: ecfp
access: public
definition: ecfp

BBa_E0020
type: SequenceAnnotation
persistent identity: annotation1478457
display id: annotation1478457
version: 1
top level: ecfp
location: https://synbiohub.org/public/igem/BBa_I15019/annotation1478457/range1478457/1
component: BBa_E0020

https://synbiohub.org/public/igem/BBa_I15019/annotation1478457/range1478457/1
type: Range
persistent identity: range1478457
display id: range1478457
version: 1
direction: direction/forward
top level: ecfp
start: 21
end: 743
orientation: inline

BBa_B0031
type: SequenceAnnotation
persistent identity: annotation1478455
display id: annotation1478455
version: 1
top level: ecfp
location: https://synbiohub.org/public/igem/BBa_I15019/annotation1478455/range1478455/1
component: BBa_B0031

https://synbiohub.org/public/igem/BBa_I15019/annotation1478455/range1478455/1
type: Range
persistent identity: range1478455
display id: range1478455
version: 1
direction: direction/forward
top level: ecfp
start: 1
end: 14
orientation: inline

BBa_K1467204
type: ComponentDefinition
persistent identity: BBa K1467204
display id: BBa_K1467204
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: GoldenGate compatible Green Flourescent Protein (GFP)
creator: Cara Deal
created: 2014-09-23T11:00:00Z
modified: 2015-06-01T07:05:42Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1846_
m user id: 4206
owner id: 21550
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: A variation of the part BBa_E0040 that has been made compatible for Golden Gate by removal of internal BsaI and BpiI recognition sequences. Fluoresces under UV light when the gene is expressed.
mutable notes: Domestication for GoldenGate by removal of internal BsaI and BpiI recognition sequences.
mutable provenance: Aequorea victoria
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence: https://synbiohub.org/public/igem/BBa_K1467204_sequence/1

BBa_K180006
type: ComponentDefinition
persistent identity: BBa K180006
display id: BBa_K180006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Game of Life - Primary plasmid (part 2) [lux pR, GFP and LacI generator]
creator: Richard Wilson, Stephanie Chambers
created: 2009-10-15T11:00:00Z
modified: 2015-05-08T01:11:06Z
discontinued: false
dominant: false
experience: experience/None
group u list: _278_
m user id: 0
owner id: 4082
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Part of the iGEM2009 Team:Southampton Game of Life primary plasmid.
mutable notes: Regulated expression of LacI (C0012) and GFP (E0040) by PLUX promoter (R0062), controlled by presence/absence of AHL-LuxR.
mutable provenance: See relevant basic biobrick parts (R0062, C0012, B0034, K180001, B0015)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0034
component: BBa_K180001
component: BBa_R0062
component: BBa_B0015
component: BBa_C0012
sequence annotation: BBa_B0034
sequence annotation: BBa_R0062
sequence annotation: BBa_B0015
sequence annotation: BBa_C0012
sequence annotation: BBa_K180001
sequence: https://synbiohub.org/public/igem/BBa_K180006_sequence/1

BBa_B0034
type: Component
persistent identity: component2267834
display id: component2267834
version: 1
top level: BBa_K180006
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K180001
type: Component
persistent identity: component2267838
display id: component2267838
version: 1
top level: BBa_K180006
access: public
definition: BBa_K180001

BBa_R0062
type: Component
persistent identity: component2267822
display id: component2267822
version: 1
top level: BBa_K180006
access: public
definition: https://synbiohub.org/public/igem/BBa_R0062/1

BBa_B0015
type: Component
persistent identity: component2267845
display id: component2267845
version: 1
top level: BBa_K180006
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_B0034
type: Component
persistent identity: component2267828
display id: component2267828
version: 1
top level: BBa_K180006
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_C0012
type: Component
persistent identity: component2267830
display id: component2267830
version: 1
top level: BBa_K180006
access: public
definition: https://synbiohub.org/public/igem/BBa_C0012/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2267834
display id: annotation2267834
version: 1
top level: BBa_K180006
location: https://synbiohub.org/public/igem/BBa_K180006/annotation2267834/range2267834/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K180006/annotation2267834/range2267834/1
type: Range
persistent identity: range2267834
display id: range2267834
version: 1
direction: direction/reverse
top level: BBa_K180006
start: 1243
end: 1254
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2267828
display id: annotation2267828
version: 1
top level: BBa_K180006
location: https://synbiohub.org/public/igem/BBa_K180006/annotation2267828/range2267828/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K180006/annotation2267828/range2267828/1
type: Range
persistent identity: range2267828
display id: range2267828
version: 1
direction: direction/reverse
top level: BBa_K180006
start: 64
end: 75
orientation: inline

BBa_R0062
type: SequenceAnnotation
persistent identity: annotation2267822
display id: annotation2267822
version: 1
top level: BBa_K180006
location: https://synbiohub.org/public/igem/BBa_K180006/annotation2267822/range2267822/1
component: BBa_R0062

https://synbiohub.org/public/igem/BBa_K180006/annotation2267822/range2267822/1
type: Range
persistent identity: range2267822
display id: range2267822
version: 1
direction: direction/forward
top level: BBa_K180006
start: 1
end: 55
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2267845
display id: annotation2267845
version: 1
top level: BBa_K180006
location: https://synbiohub.org/public/igem/BBa_K180006/annotation2267845/range2267845/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K180006/annotation2267845/range2267845/1
type: Range
persistent identity: range2267845
display id: range2267845
version: 1
direction: direction/reverse
top level: BBa_K180006
start: 2023
end: 2151
orientation: inline

BBa_C0012
type: SequenceAnnotation
persistent identity: annotation2267830
display id: annotation2267830
version: 1
top level: BBa_K180006
location: https://synbiohub.org/public/igem/BBa_K180006/annotation2267830/range2267830/1
component: BBa_C0012

https://synbiohub.org/public/igem/BBa_K180006/annotation2267830/range2267830/1
type: Range
persistent identity: range2267830
display id: range2267830
version: 1
direction: direction/forward
top level: BBa_K180006
start: 82
end: 1209
orientation: inline

BBa_K180001
type: SequenceAnnotation
persistent identity: annotation2267838
display id: annotation2267838
version: 1
top level: BBa_K180006
location: https://synbiohub.org/public/igem/BBa_K180006/annotation2267838/range2267838/1
component: BBa_K180001

https://synbiohub.org/public/igem/BBa_K180006/annotation2267838/range2267838/1
type: Range
persistent identity: range2267838
display id: range2267838
version: 1
direction: direction/reverse
top level: BBa_K180006
start: 1261
end: 2014
orientation: inline

BBa_K1323009
type: ComponentDefinition
persistent identity: BBa K1323009
display id: BBa_K1323009
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: DsRed (RFP) Coding Sequence
creator: Julia Manalil
created: 2014-10-03T11:00:00Z
modified: 2016-01-26T02:07:51Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1698_
m user id: 4206
owner id: 18368
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: TBA
mutable notes: TBA
mutable provenance: TBA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: DsRed
sequence: https://synbiohub.org/public/igem/BBa_K1323009_sequence/1

DsRed
type: SequenceAnnotation
persistent identity: annotation2428765
display id: annotation2428765
version: 1
top level: BBa_K1323009
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1323009/annotation2428765/range2428765/1

https://synbiohub.org/public/igem/BBa_K1323009/annotation2428765/range2428765/1
type: Range
persistent identity: range2428765
display id: range2428765
version: 1
direction: direction/forward
top level: BBa_K1323009
start: 1
end: 678
orientation: inline

sfCFPN
type: ComponentDefinition
persistent identity: BBa K1093004
display id: BBa_K1093004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: sfCFP N-terminal
creator: Anna Miścicka
created: 2013-09-19T11:00:00Z
modified: 2016-01-26T03:07:04Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1403_
m user id: 4206
owner id: 18431
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: Superfolder Cyan Fluorescent Protein is a derivative of superfolder Green Fluorescent protein. Compared to CFP it displays improved stability and strong cyan fluorescence. Excitation peak: 425 nm Emission peak: 475 nm. This is N-terminal of sfCFP, that can be used in Bimolecular Fluorescence Complementation system (BiFC).
mutable notes: Created by PCR on sfCFP.
mutable provenance: PartsRegistry BBa_I746909, originally from Aequorea victoria.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: sfCFP N-terminal
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_K1093004_sequence/1

sfCFP N-terminal
type: SequenceAnnotation
persistent identity: annotation2357236
display id: annotation2357236
version: 1
top level: sfCFPN
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1093004/annotation2357236/range2357236/1

https://synbiohub.org/public/igem/BBa_K1093004/annotation2357236/range2357236/1
type: Range
persistent identity: range2357236
display id: range2357236
version: 1
direction: direction/forward
top level: sfCFPN
start: 1
end: 645
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2357237
display id: annotation2357237
version: 1
top level: sfCFPN
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1093004/annotation2357237/range2357237/1

https://synbiohub.org/public/igem/BBa_K1093004/annotation2357237/range2357237/1
type: Range
persistent identity: range2357237
display id: range2357237
version: 1
direction: direction/forward
top level: sfCFPN
start: 1
end: 3
orientation: inline

BBa_K1680006
type: ComponentDefinition
persistent identity: BBa K1680006
display id: BBa_K1680006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Fluorescent protein dronpa
creator: Katharina Sporbeck
created: 2015-09-16T11:00:00Z
modified: 2016-01-26T10:15:05Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2098_
m user id: 4206
owner id: 26460
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Protein coding sequence for a mutant (K145N) of the fluorescent protein Dronpa, codon optimized for Yeast. The mutation leads to photoswitchable monomerisation/multimerisation behaviour (as described by Zhou et al. 2012).
mutable notes: Part is shipped in RFC25.
mutable provenance: gBlock designed by Team Tuebingen
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Dronpa
sequence: https://synbiohub.org/public/igem/BBa_K1680006_sequence/1

Dronpa
type: SequenceAnnotation
persistent identity: annotation2475196
display id: annotation2475196
version: 1
top level: BBa_K1680006
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1680006/annotation2475196/range2475196/1

https://synbiohub.org/public/igem/BBa_K1680006/annotation2475196/range2475196/1
type: Range
persistent identity: range2475196
display id: range2475196
version: 1
direction: direction/forward
top level: BBa_K1680006
start: 1
end: 669
orientation: inline

BBa_J52026
type: ComponentDefinition
persistent identity: BBa J52026
display id: BBa_J52026
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: dnMyD88-linker-GFP
creator: Jelka Pohar
created: 2006-10-14T11:00:00Z
modified: 2016-01-26T09:39:38Z
discontinued: false
dominant: false
group u list: _80_
m user id: 4206
owner id: 855
owning group id: 80
sample status: In stock
status: status/Available
bookmark: false
mutable description: dnMyD88 is a dominant negative form of protein MyD88 (an important protein in signaling cascade through TLR4 receptor). dnMyD88 is linked to GFP (acts as a reporter) through six aminoacid long protein linker.
mutable notes: dnMyD88-linker-GFP was made by using PCR overlap extension technique. This part is cloned in BioBrick vector pSB1AK3. Original sequence has PstI restriction site so we have to introduce a mutation without affect on amino acid sequence.
mutable provenance: dnMyD88- GFP- more to come
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: dnMyD88
sequence annotation: GFP
sequence annotation: linker
sequence annotation: dnMyD88-linker-GFP
sequence: https://synbiohub.org/public/igem/BBa_J52026_sequence/1

dnMyD88
type: SequenceAnnotation
persistent identity: annotation1903008
display id: annotation1903008
version: 1
top level: BBa_J52026
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J52026/annotation1903008/range1903008/1

https://synbiohub.org/public/igem/BBa_J52026/annotation1903008/range1903008/1
type: Range
persistent identity: range1903008
display id: range1903008
version: 1
direction: direction/forward
top level: BBa_J52026
start: 1
end: 417
orientation: inline

GFP
type: SequenceAnnotation
persistent identity: annotation1903010
display id: annotation1903010
version: 1
top level: BBa_J52026
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J52026/annotation1903010/range1903010/1

https://synbiohub.org/public/igem/BBa_J52026/annotation1903010/range1903010/1
type: Range
persistent identity: range1903010
display id: range1903010
version: 1
direction: direction/forward
top level: BBa_J52026
start: 436
end: 1155
orientation: inline

linker
type: SequenceAnnotation
persistent identity: annotation1903009
display id: annotation1903009
version: 1
top level: BBa_J52026
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_J52026/annotation1903009/range1903009/1

https://synbiohub.org/public/igem/BBa_J52026/annotation1903009/range1903009/1
type: Range
persistent identity: range1903009
display id: range1903009
version: 1
direction: direction/forward
top level: BBa_J52026
start: 418
end: 435
orientation: inline

dnMyD88-linker-GFP
type: SequenceAnnotation
persistent identity: annotation1903011
display id: annotation1903011
version: 1
top level: BBa_J52026
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_J52026/annotation1903011/range1903011/1

https://synbiohub.org/public/igem/BBa_J52026/annotation1903011/range1903011/1
type: Range
persistent identity: range1903011
display id: range1903011
version: 1
direction: direction/forward
top level: BBa_J52026
start: 1
end: 1155
orientation: inline

BBa_K900000
type: ComponentDefinition
persistent identity: BBa K900000
display id: BBa_K900000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PAmCherry (photoactivatable red fluorescent protein)
creator: Austin Jones
created: 2012-09-26T11:00:00Z
modified: 2015-05-08T01:13:42Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1165_
m user id: 0
owner id: 13597
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: PAmCHERRY is a photoactivatable red fluorescent protein. PAmCherry fluoresces when exposed to 350???400 nm light while does not fluoresce at other wavelengths. It is used a reporter tag: by expressing in cells or localizing to sub-cellular locations, one can gain spatial information within the cell by track organelles, proteins, and the whole cell itself. One can visualize the photoactivated PAmCherry with the same filter sets used for other red fluorescent proteins.
mutable notes: Codon optimization for yeast and internal restriction site removal to be compatible with our Golden Gate cloning scheme.
mutable provenance: Synthesized in house. Non-genomic DNA sequence. Mutated version of RFP.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence: https://synbiohub.org/public/igem/BBa_K900000_sequence/1

BBa_E0044
type: ComponentDefinition
persistent identity: BBa E0044
display id: BBa_E0044
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212)
creator: jcbraff
created: 2004-09-29T11:00:00Z
modified: 2015-08-31T04:07:25Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_1_
m user id: 0
owner id: 61
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: GFP-AAV [Pst1 site in earlier version knocked out]
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence: https://synbiohub.org/public/igem/BBa_E0044_sequence/1

mRFP1-NLS
type: ComponentDefinition
persistent identity: BBa K678034
display id: BBa_K678034
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: mRFP1-NLS, targeted to the nucleus
creator: DTU-Denmark-2
created: 2011-09-09T11:00:00Z
modified: 2015-05-08T01:13:04Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _882_
m user id: 0
owner id: 8341
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: mRFP
mutable notes: This part was designed for the Plug'n'Play with DNA assembly standard (RFC 80), therefore this part contains tails at the 5' and 3' ends as shown on the figure below. Please notice that mRFP1-NLS is not the same as BBa_K678030, BBa_K678031, or BBa_K678032.
mutable provenance: From p71-mRFP1 of the Center for Microbial Biotechnology plasmid collection at the Technical University of Denmark. The NLS sequence was synthesized.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: Linker 2
sequence annotation: stop
sequence annotation: NLS
sequence annotation: RFP
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_K678034_sequence/1

Linker 2
type: SequenceAnnotation
persistent identity: annotation2126904
display id: annotation2126904
version: 1
top level: mRFP1-NLS
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678034/annotation2126904/range2126904/1

https://synbiohub.org/public/igem/BBa_K678034/annotation2126904/range2126904/1
type: Range
persistent identity: range2126904
display id: range2126904
version: 1
direction: direction/forward
top level: mRFP1-NLS
start: 1
end: 8
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2126905
display id: annotation2126905
version: 1
top level: mRFP1-NLS
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K678034/annotation2126905/range2126905/1

https://synbiohub.org/public/igem/BBa_K678034/annotation2126905/range2126905/1
type: Range
persistent identity: range2126905
display id: range2126905
version: 1
direction: direction/forward
top level: mRFP1-NLS
start: 705
end: 707
orientation: inline

NLS
type: SequenceAnnotation
persistent identity: annotation2126903
display id: annotation2126903
version: 1
top level: mRFP1-NLS
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678034/annotation2126903/range2126903/1

https://synbiohub.org/public/igem/BBa_K678034/annotation2126903/range2126903/1
type: Range
persistent identity: range2126903
display id: range2126903
version: 1
direction: direction/forward
top level: mRFP1-NLS
start: 684
end: 704
orientation: inline

RFP
type: SequenceAnnotation
persistent identity: annotation2127400
display id: annotation2127400
version: 1
top level: mRFP1-NLS
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K678034/annotation2127400/range2127400/1

https://synbiohub.org/public/igem/BBa_K678034/annotation2127400/range2127400/1
type: Range
persistent identity: range2127400
display id: range2127400
version: 1
direction: direction/forward
top level: mRFP1-NLS
start: 9
end: 683
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2126906
display id: annotation2126906
version: 1
top level: mRFP1-NLS
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K678034/annotation2126906/range2126906/1

https://synbiohub.org/public/igem/BBa_K678034/annotation2126906/range2126906/1
type: Range
persistent identity: range2126906
display id: range2126906
version: 1
direction: direction/forward
top level: mRFP1-NLS
start: 9
end: 11
orientation: inline

BBa_K106004
type: ComponentDefinition
persistent identity: BBa K106004
display id: BBa_K106004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: mCherry, Aar1 AB part
creator: Ryan Quan
created: 2008-10-21T11:00:00Z
modified: 2016-01-26T09:49:59Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _226_
m user id: 4206
owner id: 3421
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This is the coding sequence for mCherry with AB ends for Aar1 cloning.
mutable notes: Since this is an AB part, there is an ATG but no stop codon. For more information on AarI cloning, please refer to the UCSF 2008 igem page.
mutable provenance: Yeast genome
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K106004_sequence/1

BBa_K1399016
type: ComponentDefinition
persistent identity: BBa K1399016
display id: BBa_K1399016
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Plac-GFP(LAA)
creator: Anna Stikane
created: 2014-09-18T11:00:00Z
modified: 2015-05-08T01:10:16Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1777_
m user id: 0
owner id: 22477
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: Lactose/IPTG inducible promoter with GFP reporter tagged with AAV-ssrA degradation tag followed by terminator. The tagged GFP is actively degraded within cell, thus provides better temporal resolution of green fluorescence and promoter activity.
mutable notes: Part was assembled using BrickClip assembly (BBF RFC104) using other biobrick parts as templates. BrickClip assembly is a special case of more general Paperclip assembly method.[1]
mutable provenance: other biobricks
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_R0010
component: BBa_B0034
component: BBa_B0015
component: BBa_K1399006
sequence annotation: BBa_B0034
sequence annotation: BBa_B0015
sequence annotation: BBa_R0010
sequence annotation: BBa_K1399006
sequence: https://synbiohub.org/public/igem/BBa_K1399016_sequence/1

BBa_R0010
type: Component
persistent identity: component2384145
display id: component2384145
version: 1
top level: BBa_K1399016
access: public
definition: https://synbiohub.org/public/igem/BBa_R0010/1

BBa_B0034
type: Component
persistent identity: component2384153
display id: component2384153
version: 1
top level: BBa_K1399016
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0015
type: Component
persistent identity: component2384166
display id: component2384166
version: 1
top level: BBa_K1399016
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_K1399006
type: Component
persistent identity: component2384159
display id: component2384159
version: 1
top level: BBa_K1399016
access: public
definition: BBa_K1399006

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2384153
display id: annotation2384153
version: 1
top level: BBa_K1399016
location: https://synbiohub.org/public/igem/BBa_K1399016/annotation2384153/range2384153/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1399016/annotation2384153/range2384153/1
type: Range
persistent identity: range2384153
display id: range2384153
version: 1
direction: direction/reverse
top level: BBa_K1399016
start: 209
end: 220
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2384166
display id: annotation2384166
version: 1
top level: BBa_K1399016
location: https://synbiohub.org/public/igem/BBa_K1399016/annotation2384166/range2384166/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K1399016/annotation2384166/range2384166/1
type: Range
persistent identity: range2384166
display id: range2384166
version: 1
direction: direction/reverse
top level: BBa_K1399016
start: 988
end: 1116
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation2384145
display id: annotation2384145
version: 1
top level: BBa_K1399016
location: https://synbiohub.org/public/igem/BBa_K1399016/annotation2384145/range2384145/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_K1399016/annotation2384145/range2384145/1
type: Range
persistent identity: range2384145
display id: range2384145
version: 1
direction: direction/forward
top level: BBa_K1399016
start: 1
end: 200
orientation: inline

BBa_K1399006
type: SequenceAnnotation
persistent identity: annotation2384159
display id: annotation2384159
version: 1
top level: BBa_K1399016
location: https://synbiohub.org/public/igem/BBa_K1399016/annotation2384159/range2384159/1
component: BBa_K1399006

https://synbiohub.org/public/igem/BBa_K1399016/annotation2384159/range2384159/1
type: Range
persistent identity: range2384159
display id: range2384159
version: 1
direction: direction/reverse
top level: BBa_K1399016
start: 227
end: 979
orientation: inline

BBa_K1486029
type: ComponentDefinition
persistent identity: BBa K1486029
display id: BBa_K1486029
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Yeast Optimized sfGFP-N + ADH1 terminator + Kanamycin resistance
creator: EPFL iGem team 2014
created: 2014-09-26T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: To edit
mutable notes: To edit
mutable provenance: To edit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K1486037
component: BBa_K1486024
component: BBa_K1486025
component: BBa_K1486028
sequence annotation: BBa_K1486024
sequence annotation: BBa_K1486025
sequence annotation: BBa_K1486037
sequence annotation: BBa_K1486028
sequence: https://synbiohub.org/public/igem/BBa_K1486029_sequence/1

BBa_K1486037
type: Component
persistent identity: component2417697
display id: component2417697
version: 1
top level: BBa_K1486029
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486037/1

BBa_K1486024
type: Component
persistent identity: component2417704
display id: component2417704
version: 1
top level: BBa_K1486029
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486024/1

BBa_K1486025
type: Component
persistent identity: component2417700
display id: component2417700
version: 1
top level: BBa_K1486029
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486025/1

BBa_K1486028
type: Component
persistent identity: component2417699
display id: component2417699
version: 1
top level: BBa_K1486029
access: public
definition: BBa_K1486028

BBa_K1486024
type: SequenceAnnotation
persistent identity: annotation2417704
display id: annotation2417704
version: 1
top level: BBa_K1486029
location: https://synbiohub.org/public/igem/BBa_K1486029/annotation2417704/range2417704/1
component: BBa_K1486024

https://synbiohub.org/public/igem/BBa_K1486029/annotation2417704/range2417704/1
type: Range
persistent identity: range2417704
display id: range2417704
version: 1
direction: direction/reverse
top level: BBa_K1486029
start: 870
end: 2226
orientation: inline

BBa_K1486025
type: SequenceAnnotation
persistent identity: annotation2417700
display id: annotation2417700
version: 1
top level: BBa_K1486029
location: https://synbiohub.org/public/igem/BBa_K1486029/annotation2417700/range2417700/1
component: BBa_K1486025

https://synbiohub.org/public/igem/BBa_K1486029/annotation2417700/range2417700/1
type: Range
persistent identity: range2417700
display id: range2417700
version: 1
direction: direction/reverse
top level: BBa_K1486029
start: 682
end: 869
orientation: inline

BBa_K1486037
type: SequenceAnnotation
persistent identity: annotation2417697
display id: annotation2417697
version: 1
top level: BBa_K1486029
location: https://synbiohub.org/public/igem/BBa_K1486029/annotation2417697/range2417697/1
component: BBa_K1486037

https://synbiohub.org/public/igem/BBa_K1486029/annotation2417697/range2417697/1
type: Range
persistent identity: range2417697
display id: range2417697
version: 1
direction: direction/reverse
top level: BBa_K1486029
start: 1
end: 39
orientation: inline

BBa_K1486028
type: SequenceAnnotation
persistent identity: annotation2417699
display id: annotation2417699
version: 1
top level: BBa_K1486029
location: https://synbiohub.org/public/igem/BBa_K1486029/annotation2417699/range2417699/1
component: BBa_K1486028

https://synbiohub.org/public/igem/BBa_K1486029/annotation2417699/range2417699/1
type: Range
persistent identity: range2417699
display id: range2417699
version: 1
direction: direction/reverse
top level: BBa_K1486029
start: 40
end: 681
orientation: inline

YFP-C
type: ComponentDefinition
persistent identity: BBa K1616016
display id: BBa_K1616016
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: YC155 - C terminal YFP split
creator: Johanna Chesnel
created: 2015-09-17T11:00:00Z
modified: 2016-01-21T02:25:51Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2033_
m user id: 4206
owner id: 22805
owning group id: 9
status: status/Planning
bookmark: false
mutable description: A split protein is a protein whose sequence has been divided into two (or more) different parts. Often used to study protein-protein interactions, the protein can not perform its function until the parts are put back together. For instance, YFP, the yellow-fluorescent protein, will only express fluorescence when its two parts will be reunited. In normal condition, the production of a protein in response to a stimulus can easily reach several hours due to the many steps required for the protein synthesis. By using split-proteins, we are taking advantage of the absence of fluorescence when the two parts are apart. Indeed, the two parts of our split-YFP, when remaining separated, can be produced without being effective. Therefore, the overall process is far less time-consuming. However, to implement a light control on the fluorescence activation, a genetic construction gathering the VVD photoreceptor and our split-YFP has to be engineered. The new alternative approach for the visualization of protein interactiosn has been developed; the biomolecular fluorescence complementation (BiFC) techniques based on the complementation between fragments of fluorescent proteins; fragments of the yellow fluorescent protein (YFP) brought together by the association of two interaction partners fused to the fragments. They noticed that the spectral characteristics of BiFC of YFP were virtually identical to those of intact YFP.(Chang-Deng Hu, 2003)
mutable notes: Illegal sites have been checked.
mutable provenance: This part works with BBa_K16160017.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: YC155
sequence: https://synbiohub.org/public/igem/BBa_K1616016_sequence/1

YC155
type: SequenceAnnotation
persistent identity: annotation2470135
display id: annotation2470135
version: 1
top level: YFP-C
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1616016/annotation2470135/range2470135/1

https://synbiohub.org/public/igem/BBa_K1616016/annotation2470135/range2470135/1
type: Range
persistent identity: range2470135
display id: range2470135
version: 1
direction: direction/forward
top level: YFP-C
start: 1
end: 255
orientation: inline

BBa_K911009
type: ComponentDefinition
persistent identity: BBa K911009
display id: BBa_K911009
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Fluorescent ratiometric construct for standardizing promoter output
creator: Fai Tsang
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:45Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _1176_
m user id: 0
owner id: 13194
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: Biosensors may give unreliable outputs. This is due to differences in the number and state of the cells from test to test. By including an internal control signal, to which another inducible signal may be normalised, the reliability and reproducibility of a sensor may be significantly improved. The construct that uses an inducible eCFP (E0020) and a constitutively expressed eYFP (E0030) under the control of the constitutive promoter Pveg (K143012). The construct is optimized in both E. coli and B. subtilis, through the use of B. subtilis ribosome binding sites GsiB (K143020) and SpoVG (K143021).
mutable notes: This biobrick is best used with a shuttle vector that can function in both E. coli and B. subtilis, which should also have preferably different antibiotic markers in E. coli and B. subtilis.
mutable provenance: This biobrick is composed entirely of existing biobricks: GsiB (K143020), eCFP (E0020), terminator (B0015), Pveg + SpoVG (K143053), eYFP (E0030) and a second terminator (B0010). It was constructed first on the E. coli- B. subitilis shuttle vector pJS130, then moved onto pSB1C3 for submission.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_B0010
component: BBa_K143020
component: BBa_B0015
component: BBa_E0030
component: BBa_K143053
component: BBa_E0020
sequence annotation: BBa_K143053
sequence annotation: BBa_B0010
sequence annotation: BBa_B0015
sequence annotation: BBa_K143020
sequence annotation: BBa_E0020
sequence annotation: BBa_E0030
sequence: https://synbiohub.org/public/igem/BBa_K911009_sequence/1

BBa_B0010
type: Component
persistent identity: component2196320
display id: component2196320
version: 1
top level: BBa_K911009
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_K143020
type: Component
persistent identity: component2196304
display id: component2196304
version: 1
top level: BBa_K911009
access: public
definition: https://synbiohub.org/public/igem/BBa_K143020/1

BBa_B0015
type: Component
persistent identity: component2196312
display id: component2196312
version: 1
top level: BBa_K911009
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_E0030
type: Component
persistent identity: component2196319
display id: component2196319
version: 1
top level: BBa_K911009
access: public
definition: eyfp

BBa_K143053
type: Component
persistent identity: component2196318
display id: component2196318
version: 1
top level: BBa_K911009
access: public
definition: https://synbiohub.org/public/igem/BBa_K143053/1

BBa_E0020
type: Component
persistent identity: component2196305
display id: component2196305
version: 1
top level: BBa_K911009
access: public
definition: ecfp

BBa_K143053
type: SequenceAnnotation
persistent identity: annotation2196318
display id: annotation2196318
version: 1
top level: BBa_K911009
location: https://synbiohub.org/public/igem/BBa_K911009/annotation2196318/range2196318/1
component: BBa_K143053

https://synbiohub.org/public/igem/BBa_K911009/annotation2196318/range2196318/1
type: Range
persistent identity: range2196318
display id: range2196318
version: 1
direction: direction/reverse
top level: BBa_K911009
start: 886
end: 1002
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2196320
display id: annotation2196320
version: 1
top level: BBa_K911009
location: https://synbiohub.org/public/igem/BBa_K911009/annotation2196320/range2196320/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K911009/annotation2196320/range2196320/1
type: Range
persistent identity: range2196320
display id: range2196320
version: 1
direction: direction/forward
top level: BBa_K911009
start: 1740
end: 1819
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2196312
display id: annotation2196312
version: 1
top level: BBa_K911009
location: https://synbiohub.org/public/igem/BBa_K911009/annotation2196312/range2196312/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K911009/annotation2196312/range2196312/1
type: Range
persistent identity: range2196312
display id: range2196312
version: 1
direction: direction/reverse
top level: BBa_K911009
start: 749
end: 877
orientation: inline

BBa_K143020
type: SequenceAnnotation
persistent identity: annotation2196304
display id: annotation2196304
version: 1
top level: BBa_K911009
location: https://synbiohub.org/public/igem/BBa_K911009/annotation2196304/range2196304/1
component: BBa_K143020

https://synbiohub.org/public/igem/BBa_K911009/annotation2196304/range2196304/1
type: Range
persistent identity: range2196304
display id: range2196304
version: 1
direction: direction/reverse
top level: BBa_K911009
start: 1
end: 11
orientation: inline

BBa_E0020
type: SequenceAnnotation
persistent identity: annotation2196305
display id: annotation2196305
version: 1
top level: BBa_K911009
location: https://synbiohub.org/public/igem/BBa_K911009/annotation2196305/range2196305/1
component: BBa_E0020

https://synbiohub.org/public/igem/BBa_K911009/annotation2196305/range2196305/1
type: Range
persistent identity: range2196305
display id: range2196305
version: 1
direction: direction/reverse
top level: BBa_K911009
start: 18
end: 740
orientation: inline

BBa_E0030
type: SequenceAnnotation
persistent identity: annotation2196319
display id: annotation2196319
version: 1
top level: BBa_K911009
location: https://synbiohub.org/public/igem/BBa_K911009/annotation2196319/range2196319/1
component: BBa_E0030

https://synbiohub.org/public/igem/BBa_K911009/annotation2196319/range2196319/1
type: Range
persistent identity: range2196319
display id: range2196319
version: 1
direction: direction/reverse
top level: BBa_K911009
start: 1009
end: 1731
orientation: inline

CFP
type: ComponentDefinition
persistent identity: BBa K157043
display id: BBa_K157043
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CFP
creator: iGEM Team Freiburg 2008
created: 2009-12-08T12:00:00Z
modified: 2015-05-08T01:10:55Z
discontinued: false
dominant: false
experience: experience/None
group u list: _41_
m user id: 0
owner id: 126
owning group id: 162
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: CFP under the control of a CMV promoter.
mutable notes: This part was split from its plasmid backbone BBa_K157040.
mutable provenance: This is a synthetic sequence.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: stop codon
sequence annotation: start codon
sequence annotation: CFP
sequence: https://synbiohub.org/public/igem/BBa_K157043_sequence/1

stop codon
type: SequenceAnnotation
persistent identity: annotation2064648
display id: annotation2064648
version: 1
top level: CFP
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K157043/annotation2064648/range2064648/1

https://synbiohub.org/public/igem/BBa_K157043/annotation2064648/range2064648/1
type: Range
persistent identity: range2064648
display id: range2064648
version: 1
direction: direction/forward
top level: CFP
start: 730
end: 732
orientation: inline

start codon
type: SequenceAnnotation
persistent identity: annotation2064647
display id: annotation2064647
version: 1
top level: CFP
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K157043/annotation2064647/range2064647/1

https://synbiohub.org/public/igem/BBa_K157043/annotation2064647/range2064647/1
type: Range
persistent identity: range2064647
display id: range2064647
version: 1
direction: direction/forward
top level: CFP
start: 1
end: 3
orientation: inline

CFP
type: SequenceAnnotation
persistent identity: annotation2064646
display id: annotation2064646
version: 1
top level: CFP
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K157043/annotation2064646/range2064646/1

https://synbiohub.org/public/igem/BBa_K157043/annotation2064646/range2064646/1
type: Range
persistent identity: range2064646
display id: range2064646
version: 1
direction: direction/forward
top level: CFP
start: 1
end: 732
orientation: inline

mCherry-GO
type: ComponentDefinition
persistent identity: BBa K678013
display id: BBa_K678013
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: mCherry-GOI
creator: DTU-Danmark-2
created: 2011-09-12T11:00:00Z
modified: 2015-05-08T01:13:04Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _882_
m user id: 0
owner id: 10556
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part was designed for the Plug'n'Play with DNA assembly standard (RFC 80), therefore this part contains tails at the 5' and 3' ends as shown on the figure below.
mutable notes: No
mutable provenance: The Danish Cancer Society
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: Linker 2
sequence annotation: mCherry-GOI
sequence annotation: Kozak
sequence: https://synbiohub.org/public/igem/BBa_K678013_sequence/1

Linker 2
type: SequenceAnnotation
persistent identity: annotation2127758
display id: annotation2127758
version: 1
top level: mCherry-GO
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678013/annotation2127758/range2127758/1

https://synbiohub.org/public/igem/BBa_K678013/annotation2127758/range2127758/1
type: Range
persistent identity: range2127758
display id: range2127758
version: 1
direction: direction/forward
top level: mCherry-GO
start: 1
end: 8
orientation: inline

mCherry-GOI
type: SequenceAnnotation
persistent identity: annotation2127759
display id: annotation2127759
version: 1
top level: mCherry-GO
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K678013/annotation2127759/range2127759/1

https://synbiohub.org/public/igem/BBa_K678013/annotation2127759/range2127759/1
type: Range
persistent identity: range2127759
display id: range2127759
version: 1
direction: direction/forward
top level: mCherry-GO
start: 16
end: 723
orientation: inline

Kozak
type: SequenceAnnotation
persistent identity: annotation2127779
display id: annotation2127779
version: 1
top level: mCherry-GO
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678013/annotation2127779/range2127779/1

https://synbiohub.org/public/igem/BBa_K678013/annotation2127779/range2127779/1
type: Range
persistent identity: range2127779
display id: range2127779
version: 1
direction: direction/forward
top level: mCherry-GO
start: 9
end: 19
orientation: inline

eYFP-TS
type: ComponentDefinition
persistent identity: BBa K678011
display id: BBa_K678011
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: eYFP-TS
creator: DTU-Denmark-2
created: 2011-09-12T11:00:00Z
modified: 2015-05-08T01:13:04Z
discontinued: false
dominant: false
experience: experience/None
group u list: _882_
m user id: 0
owner id: 10556
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part was designed for the Plug'n'Play with DNA assembly standard (RFC 80), therefore this part contains tails at the 5' and 3' ends as shown on the figure below.
mutable notes: No
mutable provenance: The Danish Cancer Society
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: eYFP-TS
sequence annotation: Kozak
sequence annotation: Linker 3
sequence: https://synbiohub.org/public/igem/BBa_K678011_sequence/1

eYFP-TS
type: SequenceAnnotation
persistent identity: annotation2127763
display id: annotation2127763
version: 1
top level: eYFP-TS
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K678011/annotation2127763/range2127763/1

https://synbiohub.org/public/igem/BBa_K678011/annotation2127763/range2127763/1
type: Range
persistent identity: range2127763
display id: range2127763
version: 1
direction: direction/forward
top level: eYFP-TS
start: 17
end: 814
orientation: inline

Kozak
type: SequenceAnnotation
persistent identity: annotation2127777
display id: annotation2127777
version: 1
top level: eYFP-TS
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678011/annotation2127777/range2127777/1

https://synbiohub.org/public/igem/BBa_K678011/annotation2127777/range2127777/1
type: Range
persistent identity: range2127777
display id: range2127777
version: 1
direction: direction/forward
top level: eYFP-TS
start: 10
end: 20
orientation: inline

Linker 3
type: SequenceAnnotation
persistent identity: annotation2127762
display id: annotation2127762
version: 1
top level: eYFP-TS
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678011/annotation2127762/range2127762/1

https://synbiohub.org/public/igem/BBa_K678011/annotation2127762/range2127762/1
type: Range
persistent identity: range2127762
display id: range2127762
version: 1
direction: direction/forward
top level: eYFP-TS
start: 1
end: 9
orientation: inline

BBa_K1033914
type: ComponentDefinition
persistent identity: BBa K1033914
display id: BBa_K1033914
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: amajLime, yellow-green chromoprotein (incl RBS & J23110)
creator: Sabri Jamal
created: 2013-09-16T11:00:00Z
modified: 2015-05-08T01:08:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1340_
m user id: 0
owner id: 13997
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This chromoprotein from the coral Anemonia majano, amajLime, naturally exhibits strong color when expressed.
mutable notes: Synthesized and codon optimized for E coli by GenScript.
mutable provenance: Anemonia majano
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_K1033916
component: BBa_J23110
component: BBa_B0034
sequence annotation: BBa_J23110
sequence annotation: BBa_K1033916
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K1033914_sequence/1

BBa_K1033916
type: Component
persistent identity: component2350107
display id: component2350107
version: 1
top level: BBa_K1033914
access: public
definition: BBa_K1033916

BBa_J23110
type: Component
persistent identity: component2350103
display id: component2350103
version: 1
top level: BBa_K1033914
access: public
definition: https://synbiohub.org/public/igem/BBa_J23110/1

BBa_B0034
type: Component
persistent identity: component2350105
display id: component2350105
version: 1
top level: BBa_K1033914
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_J23110
type: SequenceAnnotation
persistent identity: annotation2350103
display id: annotation2350103
version: 1
top level: BBa_K1033914
location: https://synbiohub.org/public/igem/BBa_K1033914/annotation2350103/range2350103/1
component: BBa_J23110

https://synbiohub.org/public/igem/BBa_K1033914/annotation2350103/range2350103/1
type: Range
persistent identity: range2350103
display id: range2350103
version: 1
direction: direction/reverse
top level: BBa_K1033914
start: 1
end: 35
orientation: inline

BBa_K1033916
type: SequenceAnnotation
persistent identity: annotation2350107
display id: annotation2350107
version: 1
top level: BBa_K1033914
location: https://synbiohub.org/public/igem/BBa_K1033914/annotation2350107/range2350107/1
component: BBa_K1033916

https://synbiohub.org/public/igem/BBa_K1033914/annotation2350107/range2350107/1
type: Range
persistent identity: range2350107
display id: range2350107
version: 1
direction: direction/reverse
top level: BBa_K1033914
start: 62
end: 754
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2350105
display id: annotation2350105
version: 1
top level: BBa_K1033914
location: https://synbiohub.org/public/igem/BBa_K1033914/annotation2350105/range2350105/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1033914/annotation2350105/range2350105/1
type: Range
persistent identity: range2350105
display id: range2350105
version: 1
direction: direction/reverse
top level: BBa_K1033914
start: 44
end: 55
orientation: inline

BBa_K1980012
type: ComponentDefinition
persistent identity: BBa K1980012
display id: BBa_K1980012
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pCopA MymT sfGFP with divergent CueR
creator: Sam Garforth
created: 2016-10-10T11:00:00Z
modified: 2016-10-23T07:47:17Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2447_
m user id: 0
m user id: 29607
owner id: 29607
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: pCopA MymT sfGFP with plasmid constitutively expressed CueR
mutable notes: dsjsdgydahjah
mutable provenance: E. coli TB
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_K1980006
component: BBa_K1980003
sequence annotation: BBa_K1980003
sequence annotation: BBa_K1980006
sequence: https://synbiohub.org/public/igem/BBa_K1980012_sequence/1

BBa_K1980006
type: Component
persistent identity: component2493045
display id: component2493045
version: 1
top level: BBa_K1980012
access: public
definition: https://synbiohub.org/public/igem/BBa_K1980006/1

BBa_K1980003
type: Component
persistent identity: component2493046
display id: component2493046
version: 1
top level: BBa_K1980012
access: public
definition: https://synbiohub.org/public/igem/BBa_K1980003/1

BBa_K1980003
type: SequenceAnnotation
persistent identity: annotation2493046
display id: annotation2493046
version: 1
top level: BBa_K1980012
location: https://synbiohub.org/public/igem/BBa_K1980012/annotation2493046/range2493046/1
component: BBa_K1980003

https://synbiohub.org/public/igem/BBa_K1980012/annotation2493046/range2493046/1
type: Range
persistent identity: range2493046
display id: range2493046
version: 1
direction: direction/reverse
top level: BBa_K1980012
start: 581
end: 1492
orientation: inline

BBa_K1980006
type: SequenceAnnotation
persistent identity: annotation2493045
display id: annotation2493045
version: 1
top level: BBa_K1980012
location: https://synbiohub.org/public/igem/BBa_K1980012/annotation2493045/range2493045/1
component: BBa_K1980006

https://synbiohub.org/public/igem/BBa_K1980012/annotation2493045/range2493045/1
type: Range
persistent identity: range2493045
display id: range2493045
version: 1
direction: direction/reverse
top level: BBa_K1980012
start: 1
end: 574
orientation: inline

BBa_K1031941
type: ComponentDefinition
persistent identity: BBa K1031941
display id: BBa_K1031941
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Pm-B0034-eGFP (XylS)
creator: He Shuaixin
created: 2013-10-20T11:00:00Z
modified: 2015-05-08T01:08:48Z
discontinued: false
dominant: false
group u list: _1338_
m user id: 0
owner id: 15843
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Pm-B0034-eGFP (XylS)
mutable notes: molecular cloning
mutable provenance: Pseudomonas.putida
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: B0034
sequence annotation: eGFP
sequence annotation: B0015
sequence annotation: Pm
sequence: https://synbiohub.org/public/igem/BBa_K1031941_sequence/1

B0034
type: SequenceAnnotation
persistent identity: annotation2370033
display id: annotation2370033
version: 1
top level: BBa_K1031941
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1031941/annotation2370033/range2370033/1

https://synbiohub.org/public/igem/BBa_K1031941/annotation2370033/range2370033/1
type: Range
persistent identity: range2370033
display id: range2370033
version: 1
direction: direction/forward
top level: BBa_K1031941
start: 89
end: 101
orientation: inline

eGFP
type: SequenceAnnotation
persistent identity: annotation2370034
display id: annotation2370034
version: 1
top level: BBa_K1031941
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1031941/annotation2370034/range2370034/1

https://synbiohub.org/public/igem/BBa_K1031941/annotation2370034/range2370034/1
type: Range
persistent identity: range2370034
display id: range2370034
version: 1
direction: direction/forward
top level: BBa_K1031941
start: 107
end: 977
orientation: inline

B0015
type: SequenceAnnotation
persistent identity: annotation2370035
display id: annotation2370035
version: 1
top level: BBa_K1031941
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1031941/annotation2370035/range2370035/1

https://synbiohub.org/public/igem/BBa_K1031941/annotation2370035/range2370035/1
type: Range
persistent identity: range2370035
display id: range2370035
version: 1
direction: direction/forward
top level: BBa_K1031941
start: 984
end: 1112
orientation: inline

Pm
type: SequenceAnnotation
persistent identity: annotation2370032
display id: annotation2370032
version: 1
top level: BBa_K1031941
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1031941/annotation2370032/range2370032/1

https://synbiohub.org/public/igem/BBa_K1031941/annotation2370032/range2370032/1
type: Range
persistent identity: range2370032
display id: range2370032
version: 1
direction: direction/forward
top level: BBa_K1031941
start: 1
end: 88
orientation: inline