//function/reporter/color
type: Collection
persistent identity: function reporter color
display id: function_reporter_color
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
owned by: james
owned by: myers
member: LacZ
member: lacZ a
member: BBa_K1033915
member: Tm-betaLac
member: BBa_K1033933
member: lacZ-alpha
member: BBa_K950005
member: BBa_K1033908
member: BBa_J04450
member: BY-Toggle
member: BBa_K648011
member: BBa_K1033903
member: BBa_K1033929
member: BBa_K1943001
member: sfGFP(Sp)
member: MelA
member: BBa_K274002
member: BBa_K1033904
member: BBa_K879313
member: BBa_K1943000
member: BBa_K879222
member: AnRe
member: fwYellow
member: gfasPurple
member: BBa_K592025
member: BBa_K648012
member: BBa_K1188000
member: BBa_K879311
member: meffBlue
member: BBa_K147002
member: BBa_K772008
member: SP-AntiNIP
member: BBa_J61032
member: BBa_K1468004
member: SP-Lipocal
member: aeBlue
member: BBa_K772103
member: BBa_K2056003
member: BBa_K1033900
member: BBa_K1033930
member: BBa_K1033914
member: asPink
member: BBa_K2056004
member: BBa_K1033931

BBa_K648012
type: ComponentDefinition
persistent identity: BBa K648012
display id: BBa_K648012
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LacZa wtih Standard 25 Prefix/Suffix
creator: Jim Rose
created: 2011-07-03T11:00:00Z
modified: 2015-05-08T01:12:59Z
discontinued: false
dominant: false
experience: experience/None
group u list: _825_
m user id: 0
owner id: 9871
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is the lacZa subunit used to complement cells for blue/white screening. It has been cloned into a vector including the prefix/suffix required for standard 25 assembly for fusion proteins.
mutable notes: This part contains the AgeI and NgoMIV sites as part of it's prefix/suffix that allows it to be used in standard 25 assembly. It was synthesized through PCR oligo synthesis methods using the following primers: Forward TATTGAATTCGCGGCCGCTTCTAGATGGCCGGCACCATGATTACGGATTCAC (57.74 oC) Reverse ATAACTGCAGCGGCCGCTACTAGTATTAACCGGTTCACTCCAGCCAGC (55.05 oC)
mutable provenance: This part was amplified out of registry stock of LacZa in order to include the standard 25 prefix/suffix
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: protein
sequence: https://synbiohub.org/public/igem/BBa_K648012_sequence/1

protein
type: SequenceAnnotation
persistent identity: annotation2122826
display id: annotation2122826
version: 1
top level: BBa_K648012
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K648012/annotation2122826/range2122826/1

https://synbiohub.org/public/igem/BBa_K648012/annotation2122826/range2122826/1
type: Range
persistent identity: range2122826
display id: range2122826
version: 1
direction: direction/forward
top level: BBa_K648012
start: 1
end: 228
orientation: inline

fwYellow
type: ComponentDefinition
persistent identity: BBa K1033910
display id: BBa_K1033910
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: fwYellow, yellow chromoprotein
creator: Sabri Jamal
created: 2013-09-16T11:00:00Z
modified: 2016-01-25T02:41:53Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1340_
m user id: 4206
owner id: 13997
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This chromoprotein naturally exhibits strong color when expressed.
mutable notes: This construct is synthetic. It was synthesized from a template where the consensus sequence for the "color dependancy" in order to obtain a chromoprotein with a unique yellow color.
mutable provenance: Synthetic chromoprotein from DNA 2.0.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: fwYellow
sequence: https://synbiohub.org/public/igem/BBa_K1033910_sequence/1

fwYellow
type: SequenceAnnotation
persistent identity: annotation2371218
display id: annotation2371218
version: 1
top level: fwYellow
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1033910/annotation2371218/range2371218/1

https://synbiohub.org/public/igem/BBa_K1033910/annotation2371218/range2371218/1
type: Range
persistent identity: range2371218
display id: range2371218
version: 1
direction: direction/forward
top level: fwYellow
start: 1
end: 711
orientation: inline

BBa_K648011
type: ComponentDefinition
persistent identity: BBa K648011
display id: BBa_K648011
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Standard 25-Ready Xyle Reporter
creator: Jim Rose
created: 2011-07-03T11:00:00Z
modified: 2015-05-08T01:12:59Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _825_
m user id: 0
owner id: 9871
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: This is a mutated version of the Xyle reporter gene which encodes for the enzyme catechol-2,3-dioxygenase (metapyrocatechase), which converts catechol to the bright yellow product 2-hydroxy-cis,cis-muconic semialdehyde. This version of Xyle has been made compatible with standard 25 assembly methods by removing three restriction sites (two NgoMIV sites: at bp 315 and 486, as well as one AgeI site: at bp 837). These mutations were made synonymous with the original sequence and codon optimized for E. Coli. Because of the synonymous mutations, this gene can be easily used to create fusion protein parts. For more information on the Xyle gene and its uses as a reporter see part BBa_J33204.
mutable notes: The mutations in this gene were made so that they were synonymous and codon optimized for E. coli cells. The part also contains the prefix/suffix for standard 25 assembly with addition restriction sites NgoMIV and AgeI.
mutable provenance: The original genetic material for this part came from part BBa_K316007 which was mutated via PCR based site-directed mutagensis. The xyle portion of this large part was then amplified via PCR reaction and cloned into a new vector along with a prefix/suffix for standard 25 assembly.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: misc
sequence: https://synbiohub.org/public/igem/BBa_K648011_sequence/1

misc
type: SequenceAnnotation
persistent identity: annotation2122825
display id: annotation2122825
version: 1
top level: BBa_K648011
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K648011/annotation2122825/range2122825/1

https://synbiohub.org/public/igem/BBa_K648011/annotation2122825/range2122825/1
type: Range
persistent identity: range2122825
display id: range2122825
version: 1
direction: direction/forward
top level: BBa_K648011
start: 1
end: 918
orientation: inline

Tm-betaLac
type: ComponentDefinition
persistent identity: BBa K157018
display id: BBa_K157018
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Tm-betaLactamaseBla1
creator: iGEM Team Freiburg 2008
created: 2008-10-25T11:00:00Z
modified: 2015-05-08T01:10:54Z
discontinued: false
dominant: false
experience: experience/None
group u list: _232_
m user id: 0
owner id: 1673
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: First fragment of beta-Lactamase fused to the trans-membrane region of the Erbb1/EGF-receptor.
mutable notes: none
mutable provenance: Derived by cloning of the parts BBa_K157002, BBa_I757011. For further information see the description of the parts mentioned above.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: Scar (Thr, Gly)
sequence annotation: Tm-betaLactamase(Bla1)
sequence annotation: NgoMI
sequence annotation: AgeI
sequence annotation: Tm
sequence annotation: Bla1
sequence: https://synbiohub.org/public/igem/BBa_K157018_sequence/1

Scar (Thr, Gly)
type: SequenceAnnotation
persistent identity: annotation1991372
display id: annotation1991372
version: 1
top level: Tm-betaLac
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157018/annotation1991372/range1991372/1

https://synbiohub.org/public/igem/BBa_K157018/annotation1991372/range1991372/1
type: Range
persistent identity: range1991372
display id: range1991372
version: 1
direction: direction/forward
top level: Tm-betaLac
start: 79
end: 84
orientation: inline

Tm-betaLactamase(Bla1)
type: SequenceAnnotation
persistent identity: annotation1989985
display id: annotation1989985
version: 1
top level: Tm-betaLac
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K157018/annotation1989985/range1989985/1

https://synbiohub.org/public/igem/BBa_K157018/annotation1989985/range1989985/1
type: Range
persistent identity: range1989985
display id: range1989985
version: 1
direction: direction/forward
top level: Tm-betaLac
start: 1
end: 615
orientation: inline

NgoMI
type: SequenceAnnotation
persistent identity: annotation1989983
display id: annotation1989983
version: 1
top level: Tm-betaLac
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157018/annotation1989983/range1989983/1

https://synbiohub.org/public/igem/BBa_K157018/annotation1989983/range1989983/1
type: Range
persistent identity: range1989983
display id: range1989983
version: 1
direction: direction/forward
top level: Tm-betaLac
start: 4
end: 9
orientation: inline

AgeI
type: SequenceAnnotation
persistent identity: annotation1989984
display id: annotation1989984
version: 1
top level: Tm-betaLac
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157018/annotation1989984/range1989984/1

https://synbiohub.org/public/igem/BBa_K157018/annotation1989984/range1989984/1
type: Range
persistent identity: range1989984
display id: range1989984
version: 1
direction: direction/forward
top level: Tm-betaLac
start: 610
end: 615
orientation: inline

Tm
type: SequenceAnnotation
persistent identity: annotation1991288
display id: annotation1991288
version: 1
top level: Tm-betaLac
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157018/annotation1991288/range1991288/1

https://synbiohub.org/public/igem/BBa_K157018/annotation1991288/range1991288/1
type: Range
persistent identity: range1991288
display id: range1991288
version: 1
direction: direction/forward
top level: Tm-betaLac
start: 10
end: 78
orientation: inline

Bla1
type: SequenceAnnotation
persistent identity: annotation1991421
display id: annotation1991421
version: 1
top level: Tm-betaLac
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157018/annotation1991421/range1991421/1

https://synbiohub.org/public/igem/BBa_K157018/annotation1991421/range1991421/1
type: Range
persistent identity: range1991421
display id: range1991421
version: 1
direction: direction/forward
top level: Tm-betaLac
start: 85
end: 609
orientation: inline

SP-AntiNIP
type: ComponentDefinition
persistent identity: BBa K157024
display id: BBa_K157024
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: SP-AntiNIP-GGGSlinker-Tm-betaLactamase(Bla1)
creator: iGEM Team Freiburg 2008
created: 2008-10-25T11:00:00Z
modified: 2015-05-08T01:10:55Z
discontinued: false
dominant: false
experience: experience/None
group u list: _232_
m user id: 0
owner id: 1673
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: A composite part consisting of the signal-peptide of Erbb1/EGF-receptor, scFV-antiNIP, GGGSflexlinker (extracellular part), the trans-membrane region of the Erbb1/EGF-receptor and the N-terminal part of split-enzyme beta-Lactamase (intracellular part).
mutable notes: none
mutable provenance: Derived by cloning of the parts BBa_K157001, BBa_K157003, BBa_K157013, BBa_K157002 and BBa_I757011. For further information see the description of the parts mentioned above.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: SP
sequence annotation: Scar (Thr, Gly)
sequence annotation: Bla1
sequence annotation: GGGS-Linker
sequence annotation: SP-AntiNIP-GGGSlinker-Tm-betaLactamase(Bla1)
sequence annotation: NgoMI
sequence annotation: Sc-Fv_anti-NIP
sequence annotation: Tm
sequence annotation: AgeI
sequence: https://synbiohub.org/public/igem/BBa_K157024_sequence/1

SP
type: SequenceAnnotation
persistent identity: annotation1992602
display id: annotation1992602
version: 1
top level: SP-AntiNIP
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157024/annotation1992602/range1992602/1

https://synbiohub.org/public/igem/BBa_K157024/annotation1992602/range1992602/1
type: Range
persistent identity: range1992602
display id: range1992602
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 10
end: 81
orientation: inline

Scar (Thr, Gly)
type: SequenceAnnotation
persistent identity: annotation1992603
display id: annotation1992603
version: 1
top level: SP-AntiNIP
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157024/annotation1992603/range1992603/1

https://synbiohub.org/public/igem/BBa_K157024/annotation1992603/range1992603/1
type: Range
persistent identity: range1992603
display id: range1992603
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 82
end: 87
orientation: inline

Bla1
type: SequenceAnnotation
persistent identity: annotation1992613
display id: annotation1992613
version: 1
top level: SP-AntiNIP
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157024/annotation1992613/range1992613/1

https://synbiohub.org/public/igem/BBa_K157024/annotation1992613/range1992613/1
type: Range
persistent identity: range1992613
display id: range1992613
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 955
end: 1479
orientation: inline

Scar (Thr, Gly)
type: SequenceAnnotation
persistent identity: annotation1992610
display id: annotation1992610
version: 1
top level: SP-AntiNIP
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157024/annotation1992610/range1992610/1

https://synbiohub.org/public/igem/BBa_K157024/annotation1992610/range1992610/1
type: Range
persistent identity: range1992610
display id: range1992610
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 874
end: 879
orientation: inline

Scar (Thr, Gly)
type: SequenceAnnotation
persistent identity: annotation1992606
display id: annotation1992606
version: 1
top level: SP-AntiNIP
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157024/annotation1992606/range1992606/1

https://synbiohub.org/public/igem/BBa_K157024/annotation1992606/range1992606/1
type: Range
persistent identity: range1992606
display id: range1992606
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 823
end: 828
orientation: inline

GGGS-Linker
type: SequenceAnnotation
persistent identity: annotation1992608
display id: annotation1992608
version: 1
top level: SP-AntiNIP
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157024/annotation1992608/range1992608/1

https://synbiohub.org/public/igem/BBa_K157024/annotation1992608/range1992608/1
type: Range
persistent identity: range1992608
display id: range1992608
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 829
end: 873
orientation: inline

SP-AntiNIP-GGGSlinker-Tm-betaLactamase(Bla1)
type: SequenceAnnotation
persistent identity: annotation1990168
display id: annotation1990168
version: 1
top level: SP-AntiNIP
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K157024/annotation1990168/range1990168/1

https://synbiohub.org/public/igem/BBa_K157024/annotation1990168/range1990168/1
type: Range
persistent identity: range1990168
display id: range1990168
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 1
end: 1485
orientation: inline

NgoMI
type: SequenceAnnotation
persistent identity: annotation1990166
display id: annotation1990166
version: 1
top level: SP-AntiNIP
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157024/annotation1990166/range1990166/1

https://synbiohub.org/public/igem/BBa_K157024/annotation1990166/range1990166/1
type: Range
persistent identity: range1990166
display id: range1990166
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 4
end: 9
orientation: inline

Scar (Thr, Gly)
type: SequenceAnnotation
persistent identity: annotation1992612
display id: annotation1992612
version: 1
top level: SP-AntiNIP
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157024/annotation1992612/range1992612/1

https://synbiohub.org/public/igem/BBa_K157024/annotation1992612/range1992612/1
type: Range
persistent identity: range1992612
display id: range1992612
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 949
end: 954
orientation: inline

Sc-Fv_anti-NIP
type: SequenceAnnotation
persistent identity: annotation1992604
display id: annotation1992604
version: 1
top level: SP-AntiNIP
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157024/annotation1992604/range1992604/1

https://synbiohub.org/public/igem/BBa_K157024/annotation1992604/range1992604/1
type: Range
persistent identity: range1992604
display id: range1992604
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 88
end: 822
orientation: inline

Tm
type: SequenceAnnotation
persistent identity: annotation1992611
display id: annotation1992611
version: 1
top level: SP-AntiNIP
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157024/annotation1992611/range1992611/1

https://synbiohub.org/public/igem/BBa_K157024/annotation1992611/range1992611/1
type: Range
persistent identity: range1992611
display id: range1992611
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 880
end: 948
orientation: inline

AgeI
type: SequenceAnnotation
persistent identity: annotation1990167
display id: annotation1990167
version: 1
top level: SP-AntiNIP
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157024/annotation1990167/range1990167/1

https://synbiohub.org/public/igem/BBa_K157024/annotation1990167/range1990167/1
type: Range
persistent identity: range1990167
display id: range1990167
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 1480
end: 1485
orientation: inline

lacZ a
type: ComponentDefinition
persistent identity: BBa E0033
display id: BBa_E0033
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LacZ alpha fragment; complements matching N-terminal deletion mutant (lacZ-omega)
creator: Yong-Su Jin (Fighting Darwins)
created: 2004-01-27T12:00:00Z
modified: 2015-08-31T04:07:25Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable description: to greatly reduce the number of bases, this is only a portion of the LacZ gene
mutable notes: Restriction sites (modified) 76-79 (gcc to gca) 79-81 (gct to gca) 85-87 (gaa to gag) 106-108 (tgc to tgt) 109-111(agg to aga)
mutable provenance: www.ncbi.nlm.nih.gov
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: C
sequence annotation: G
sequence annotation: lacZ gene fragment
sequence annotation: A
sequence annotation: T7 promoter
sequence annotation: T3 promoter
sequence annotation: T
sequence annotation: lacZ alpha
sequence: https://synbiohub.org/public/igem/BBa_E0033_sequence/1

C
type: SequenceAnnotation
persistent identity: annotation308387
display id: annotation308387
version: 1
top level: lacZ a
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_E0033/annotation308387/range308387/1

https://synbiohub.org/public/igem/BBa_E0033/annotation308387/range308387/1
type: Range
persistent identity: range308387
display id: range308387
version: 1
direction: direction/forward
top level: lacZ a
start: 78
end: 78
orientation: inline

G
type: SequenceAnnotation
persistent identity: annotation308388
display id: annotation308388
version: 1
top level: lacZ a
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_E0033/annotation308388/range308388/1

https://synbiohub.org/public/igem/BBa_E0033/annotation308388/range308388/1
type: Range
persistent identity: range308388
display id: range308388
version: 1
direction: direction/forward
top level: lacZ a
start: 111
end: 111
orientation: inline

lacZ gene fragment
type: SequenceAnnotation
persistent identity: annotation1938941
display id: annotation1938941
version: 1
top level: lacZ a
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_E0033/annotation1938941/range1938941/1

https://synbiohub.org/public/igem/BBa_E0033/annotation1938941/range1938941/1
type: Range
persistent identity: range1938941
display id: range1938941
version: 1
direction: direction/forward
top level: lacZ a
start: 199
end: 348
orientation: inline

A
type: SequenceAnnotation
persistent identity: annotation308381
display id: annotation308381
version: 1
top level: lacZ a
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_E0033/annotation308381/range308381/1

https://synbiohub.org/public/igem/BBa_E0033/annotation308381/range308381/1
type: Range
persistent identity: range308381
display id: range308381
version: 1
direction: direction/forward
top level: lacZ a
start: 87
end: 87
orientation: inline

lacZ gene fragment
type: SequenceAnnotation
persistent identity: annotation1938942
display id: annotation1938942
version: 1
top level: lacZ a
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_E0033/annotation1938942/range1938942/1

https://synbiohub.org/public/igem/BBa_E0033/annotation1938942/range1938942/1
type: Range
persistent identity: range1938942
display id: range1938942
version: 1
direction: direction/forward
top level: lacZ a
start: 1
end: 15
orientation: inline

T7 promoter
type: SequenceAnnotation
persistent identity: annotation1938940
display id: annotation1938940
version: 1
top level: lacZ a
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_E0033/annotation1938940/range1938940/1

https://synbiohub.org/public/igem/BBa_E0033/annotation1938940/range1938940/1
type: Range
persistent identity: range1938940
display id: range1938940
version: 1
direction: direction/reverse
top level: lacZ a
start: 173
end: 191
orientation: inline

C
type: SequenceAnnotation
persistent identity: annotation308383
display id: annotation308383
version: 1
top level: lacZ a
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_E0033/annotation308383/range308383/1

https://synbiohub.org/public/igem/BBa_E0033/annotation308383/range308383/1
type: Range
persistent identity: range308383
display id: range308383
version: 1
direction: direction/forward
top level: lacZ a
start: 108
end: 108
orientation: inline

T3 promoter
type: SequenceAnnotation
persistent identity: annotation1938939
display id: annotation1938939
version: 1
top level: lacZ a
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_E0033/annotation1938939/range1938939/1

https://synbiohub.org/public/igem/BBa_E0033/annotation1938939/range1938939/1
type: Range
persistent identity: range1938939
display id: range1938939
version: 1
direction: direction/forward
top level: lacZ a
start: 26
end: 35
orientation: inline

T
type: SequenceAnnotation
persistent identity: annotation308375
display id: annotation308375
version: 1
top level: lacZ a
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_E0033/annotation308375/range308375/1

https://synbiohub.org/public/igem/BBa_E0033/annotation308375/range308375/1
type: Range
persistent identity: range308375
display id: range308375
version: 1
direction: direction/forward
top level: lacZ a
start: 81
end: 81
orientation: inline

lacZ alpha
type: SequenceAnnotation
persistent identity: annotation308320
display id: annotation308320
version: 1
top level: lacZ a
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_E0033/annotation308320/range308320/1

https://synbiohub.org/public/igem/BBa_E0033/annotation308320/range308320/1
type: Range
persistent identity: range308320
display id: range308320
version: 1
direction: direction/forward
top level: lacZ a
start: 1
end: 348
orientation: inline

BBa_K950005
type: ComponentDefinition
persistent identity: BBa K950005
display id: BBa_K950005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: lacZ
creator: Jan Rudolph
created: 2012-07-28T11:00:00Z
modified: 2015-05-08T01:13:49Z
discontinued: false
dominant: false
group u list: _1218_
m user id: 0
owner id: 13191
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: lacZ can be used as reporter gene
mutable notes: no Xba1 Spe1 sites found
mutable provenance: plasmid from Jansen lab Tuebingen University
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: lacZ
sequence: https://synbiohub.org/public/igem/BBa_K950005_sequence/1

lacZ
type: SequenceAnnotation
persistent identity: annotation2179274
display id: annotation2179274
version: 1
top level: BBa_K950005
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K950005/annotation2179274/range2179274/1

https://synbiohub.org/public/igem/BBa_K950005/annotation2179274/range2179274/1
type: Range
persistent identity: range2179274
display id: range2179274
version: 1
direction: direction/forward
top level: BBa_K950005
start: 1
end: 2514
orientation: inline

BBa_K2056004
type: ComponentDefinition
persistent identity: BBa K2056004
display id: BBa_K2056004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: NOx(Oxides of Nitrogen) sensor device (pNirK-Yellow chromoprotein)
creator: Muhammad Ismail
created: 2016-10-12T11:00:00Z
modified: 2016-10-13T01:10:17Z
discontinued: false
dominant: false
group u list: _2524_
m user id: 33019
owner id: 33019
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This part is the oxides of nitrogen (NOx) sensing module of the 'Inspector NOxCO' device. 'Inspector NOxCO' is a bacterial sensor for carbon monoxide (CO) and oxides of nitrogen (NOx) in vehicle emissions. This part consists of the Anderson constitutive promoter(BBa_J23110) and RBS(B0030) with the coding sequence for NsrR(K2056001) a NOx sensitive repressor protein and single terminator(B0010) followed by NsrR repressed promoter pNirk(k2056002) with RBS(B0030) and 'Yellow chromoprotein'(K1033910) with terminator(BBa_B0012). If NOx are present, they will bind to the constitutive expressed NsrR that will relieve repression on the pNirK promoter activating transcription of the yellow chromoprotein.
mutable notes: The selection of the RBS and Constitutive promoter. Because of limitations of repeats that could be synthesized, we had to give up on the double terminators in the original design and go with single different terminators. A 70bp spacer(B0040) has been added for transcriptional efficiency.
mutable provenance: All parts in this sequence come from the Registry and have been listed. However, the complete part has been synthesized from IDT using their free gBLocks offer.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
component: BBa_B0012
component: BBa_K2056002
component: BBa_K1033910
component: BBa_B0030
component: BBa_B0010
component: BBa_B0040
component: BBa_K2056001
component: BBa_J23110
sequence annotation: BBa_B0030
sequence annotation: BBa_B0012
sequence annotation: BBa_K1033910
sequence annotation: BBa_K2056002
sequence annotation: BBa_J23110
sequence annotation: BBa_K2056001
sequence annotation: BBa_B0040
sequence annotation: BBa_B0010
sequence: https://synbiohub.org/public/igem/BBa_K2056004_sequence/1

BBa_B0012
type: Component
persistent identity: component2499278
display id: component2499278
version: 1
top level: BBa_K2056004
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_K2056002
type: Component
persistent identity: component2499272
display id: component2499272
version: 1
top level: BBa_K2056004
access: public
definition: https://synbiohub.org/public/igem/BBa_K2056002/1

BBa_K1033910
type: Component
persistent identity: component2499277
display id: component2499277
version: 1
top level: BBa_K2056004
access: public
definition: fwYellow

BBa_B0030
type: Component
persistent identity: component2499274
display id: component2499274
version: 1
top level: BBa_K2056004
access: public
definition: https://synbiohub.org/public/igem/BBa_B0030/1

BBa_B0010
type: Component
persistent identity: component2499268
display id: component2499268
version: 1
top level: BBa_K2056004
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0040
type: Component
persistent identity: component2499271
display id: component2499271
version: 1
top level: BBa_K2056004
access: public
definition: https://synbiohub.org/public/igem/BBa_B0040/1

BBa_K2056001
type: Component
persistent identity: component2499267
display id: component2499267
version: 1
top level: BBa_K2056004
access: public
definition: https://synbiohub.org/public/igem/BBa_K2056001/1

BBa_B0030
type: Component
persistent identity: component2499265
display id: component2499265
version: 1
top level: BBa_K2056004
access: public
definition: https://synbiohub.org/public/igem/BBa_B0030/1

BBa_J23110
type: Component
persistent identity: component2499263
display id: component2499263
version: 1
top level: BBa_K2056004
access: public
definition: https://synbiohub.org/public/igem/BBa_J23110/1

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation2499274
display id: annotation2499274
version: 1
top level: BBa_K2056004
location: https://synbiohub.org/public/igem/BBa_K2056004/annotation2499274/range2499274/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_K2056004/annotation2499274/range2499274/1
type: Range
persistent identity: range2499274
display id: range2499274
version: 1
direction: direction/forward
top level: BBa_K2056004
start: 987
end: 1001
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2499278
display id: annotation2499278
version: 1
top level: BBa_K2056004
location: https://synbiohub.org/public/igem/BBa_K2056004/annotation2499278/range2499278/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K2056004/annotation2499278/range2499278/1
type: Range
persistent identity: range2499278
display id: range2499278
version: 1
direction: direction/forward
top level: BBa_K2056004
start: 1730
end: 1770
orientation: inline

BBa_K1033910
type: SequenceAnnotation
persistent identity: annotation2499277
display id: annotation2499277
version: 1
top level: BBa_K2056004
location: https://synbiohub.org/public/igem/BBa_K2056004/annotation2499277/range2499277/1
component: BBa_K1033910

https://synbiohub.org/public/igem/BBa_K2056004/annotation2499277/range2499277/1
type: Range
persistent identity: range2499277
display id: range2499277
version: 1
direction: direction/reverse
top level: BBa_K2056004
start: 1008
end: 1721
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation2499265
display id: annotation2499265
version: 1
top level: BBa_K2056004
location: https://synbiohub.org/public/igem/BBa_K2056004/annotation2499265/range2499265/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_K2056004/annotation2499265/range2499265/1
type: Range
persistent identity: range2499265
display id: range2499265
version: 1
direction: direction/forward
top level: BBa_K2056004
start: 44
end: 58
orientation: inline

BBa_K2056002
type: SequenceAnnotation
persistent identity: annotation2499272
display id: annotation2499272
version: 1
top level: BBa_K2056004
location: https://synbiohub.org/public/igem/BBa_K2056004/annotation2499272/range2499272/1
component: BBa_K2056002

https://synbiohub.org/public/igem/BBa_K2056004/annotation2499272/range2499272/1
type: Range
persistent identity: range2499272
display id: range2499272
version: 1
direction: direction/reverse
top level: BBa_K2056004
start: 667
end: 978
orientation: inline

BBa_J23110
type: SequenceAnnotation
persistent identity: annotation2499263
display id: annotation2499263
version: 1
top level: BBa_K2056004
location: https://synbiohub.org/public/igem/BBa_K2056004/annotation2499263/range2499263/1
component: BBa_J23110

https://synbiohub.org/public/igem/BBa_K2056004/annotation2499263/range2499263/1
type: Range
persistent identity: range2499263
display id: range2499263
version: 1
direction: direction/reverse
top level: BBa_K2056004
start: 1
end: 35
orientation: inline

BBa_K2056001
type: SequenceAnnotation
persistent identity: annotation2499267
display id: annotation2499267
version: 1
top level: BBa_K2056004
location: https://synbiohub.org/public/igem/BBa_K2056004/annotation2499267/range2499267/1
component: BBa_K2056001

https://synbiohub.org/public/igem/BBa_K2056004/annotation2499267/range2499267/1
type: Range
persistent identity: range2499267
display id: range2499267
version: 1
direction: direction/reverse
top level: BBa_K2056004
start: 67
end: 492
orientation: inline

BBa_B0040
type: SequenceAnnotation
persistent identity: annotation2499271
display id: annotation2499271
version: 1
top level: BBa_K2056004
location: https://synbiohub.org/public/igem/BBa_K2056004/annotation2499271/range2499271/1
component: BBa_B0040

https://synbiohub.org/public/igem/BBa_K2056004/annotation2499271/range2499271/1
type: Range
persistent identity: range2499271
display id: range2499271
version: 1
direction: direction/forward
top level: BBa_K2056004
start: 589
end: 658
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2499268
display id: annotation2499268
version: 1
top level: BBa_K2056004
location: https://synbiohub.org/public/igem/BBa_K2056004/annotation2499268/range2499268/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K2056004/annotation2499268/range2499268/1
type: Range
persistent identity: range2499268
display id: range2499268
version: 1
direction: direction/forward
top level: BBa_K2056004
start: 501
end: 580
orientation: inline

BBa_K1033914
type: ComponentDefinition
persistent identity: BBa K1033914
display id: BBa_K1033914
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: amajLime, yellow-green chromoprotein (incl RBS & J23110)
creator: Sabri Jamal
created: 2013-09-16T11:00:00Z
modified: 2015-05-08T01:08:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1340_
m user id: 0
owner id: 13997
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This chromoprotein from the coral Anemonia majano, amajLime, naturally exhibits strong color when expressed.
mutable notes: Synthesized and codon optimized for E coli by GenScript.
mutable provenance: Anemonia majano
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_K1033916
component: BBa_B0034
component: BBa_J23110
sequence annotation: BBa_J23110
sequence annotation: BBa_K1033916
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K1033914_sequence/1

BBa_K1033916
type: Component
persistent identity: component2350107
display id: component2350107
version: 1
top level: BBa_K1033914
access: public
definition: https://synbiohub.org/public/igem/BBa_K1033916/1

BBa_B0034
type: Component
persistent identity: component2350105
display id: component2350105
version: 1
top level: BBa_K1033914
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_J23110
type: Component
persistent identity: component2350103
display id: component2350103
version: 1
top level: BBa_K1033914
access: public
definition: https://synbiohub.org/public/igem/BBa_J23110/1

BBa_J23110
type: SequenceAnnotation
persistent identity: annotation2350103
display id: annotation2350103
version: 1
top level: BBa_K1033914
location: https://synbiohub.org/public/igem/BBa_K1033914/annotation2350103/range2350103/1
component: BBa_J23110

https://synbiohub.org/public/igem/BBa_K1033914/annotation2350103/range2350103/1
type: Range
persistent identity: range2350103
display id: range2350103
version: 1
direction: direction/reverse
top level: BBa_K1033914
start: 1
end: 35
orientation: inline

BBa_K1033916
type: SequenceAnnotation
persistent identity: annotation2350107
display id: annotation2350107
version: 1
top level: BBa_K1033914
location: https://synbiohub.org/public/igem/BBa_K1033914/annotation2350107/range2350107/1
component: BBa_K1033916

https://synbiohub.org/public/igem/BBa_K1033914/annotation2350107/range2350107/1
type: Range
persistent identity: range2350107
display id: range2350107
version: 1
direction: direction/reverse
top level: BBa_K1033914
start: 62
end: 754
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2350105
display id: annotation2350105
version: 1
top level: BBa_K1033914
location: https://synbiohub.org/public/igem/BBa_K1033914/annotation2350105/range2350105/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1033914/annotation2350105/range2350105/1
type: Range
persistent identity: range2350105
display id: range2350105
version: 1
direction: direction/reverse
top level: BBa_K1033914
start: 44
end: 55
orientation: inline

BBa_K1033931
type: ComponentDefinition
persistent identity: BBa K1033931
display id: BBa_K1033931
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: amilGFP, yellow chromoprotein (incl RBS)
creator: Erik Lundin
created: 2013-09-16T11:00:00Z
modified: 2015-05-08T01:08:50Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1340_
m user id: 0
owner id: 13997
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This chromoprotein from the coral Acropora millepora, amilGFP, naturally exhibits strong yellow color when expressed. The color is readily visible to naked eye both in LB-culture and on agar plates. Color development can be seen in less than 24 hours of incubation.
mutable notes: Two illegal internal restriction sites (EcoRI and PstI) were removed.
mutable provenance: Acropora millepora. The DNA was provided by Jeffrey Miller at UCLA, and was made BioBrick-compatible by removing two illegal internal restriction sites (EcoRI and PstI).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_K592010
component: BBa_B0034
sequence annotation: BBa_K592010
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K1033931_sequence/1

BBa_K592010
type: Component
persistent identity: component2351898
display id: component2351898
version: 1
top level: BBa_K1033931
access: public
definition: https://synbiohub.org/public/igem/BBa_K592010/1

BBa_B0034
type: Component
persistent identity: component2351896
display id: component2351896
version: 1
top level: BBa_K1033931
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K592010
type: SequenceAnnotation
persistent identity: annotation2351898
display id: annotation2351898
version: 1
top level: BBa_K1033931
location: https://synbiohub.org/public/igem/BBa_K1033931/annotation2351898/range2351898/1
component: BBa_K592010

https://synbiohub.org/public/igem/BBa_K1033931/annotation2351898/range2351898/1
type: Range
persistent identity: range2351898
display id: range2351898
version: 1
direction: direction/reverse
top level: BBa_K1033931
start: 19
end: 717
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2351896
display id: annotation2351896
version: 1
top level: BBa_K1033931
location: https://synbiohub.org/public/igem/BBa_K1033931/annotation2351896/range2351896/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1033931/annotation2351896/range2351896/1
type: Range
persistent identity: range2351896
display id: range2351896
version: 1
direction: direction/reverse
top level: BBa_K1033931
start: 1
end: 12
orientation: inline

BBa_K1943001
type: ComponentDefinition
persistent identity: BBa K1943001
display id: BBa_K1943001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: fwYellow, yellow chromoprotein reporter system (medium Promoter , weak RBS)
creator: Shixin Lu, Shiqiang Tang
created: 2016-08-28T11:00:00Z
modified: 2016-10-21T08:02:33Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2410_
m user id: 30006
owner id: 29845
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Team Uppsala 2012 chromoprotein attracts many interests because it's more convenient than fluorescent protein for it can be observed with naked eyes. However, characterized data of these proteins are few. Because a single coding region is on the plasmid, we constructed BBa_K1943001 with chromoprotein fwYellow(BBa_K1033910) this year and did characterization. We've constructed a series of plasmids which are all in the same pattern: a strong/weak promoter, a strong/weak RBS and a chromoprotein. We want to monitor the speed of expression of these plasmids in normal incubation conditions (like 37℃ overnight for LB agar plate and 37℃ 180rpm for LB broth). The expression speed and strength of the constructed biobricks will be carefully monitored and gave others a relative scale for using chromoprotein as a reporter gene.
mutable notes: no
mutable provenance: basic parts
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_B0015
component: BBa_K1033910
component: BBa_K608007
sequence annotation: BBa_K1033910
sequence annotation: BBa_B0015
sequence annotation: BBa_K608007
sequence: https://synbiohub.org/public/igem/BBa_K1943001_sequence/1

BBa_B0015
type: Component
persistent identity: component2481880
display id: component2481880
version: 1
top level: BBa_K1943001
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_K1033910
type: Component
persistent identity: component2481873
display id: component2481873
version: 1
top level: BBa_K1943001
access: public
definition: fwYellow

BBa_K608007
type: Component
persistent identity: component2481871
display id: component2481871
version: 1
top level: BBa_K1943001
access: public
definition: https://synbiohub.org/public/igem/BBa_K608007/1

BBa_K1033910
type: SequenceAnnotation
persistent identity: annotation2481873
display id: annotation2481873
version: 1
top level: BBa_K1943001
location: https://synbiohub.org/public/igem/BBa_K1943001/annotation2481873/range2481873/1
component: BBa_K1033910

https://synbiohub.org/public/igem/BBa_K1943001/annotation2481873/range2481873/1
type: Range
persistent identity: range2481873
display id: range2481873
version: 1
direction: direction/reverse
top level: BBa_K1943001
start: 64
end: 777
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2481880
display id: annotation2481880
version: 1
top level: BBa_K1943001
location: https://synbiohub.org/public/igem/BBa_K1943001/annotation2481880/range2481880/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K1943001/annotation2481880/range2481880/1
type: Range
persistent identity: range2481880
display id: range2481880
version: 1
direction: direction/reverse
top level: BBa_K1943001
start: 786
end: 914
orientation: inline

BBa_K608007
type: SequenceAnnotation
persistent identity: annotation2481871
display id: annotation2481871
version: 1
top level: BBa_K1943001
location: https://synbiohub.org/public/igem/BBa_K1943001/annotation2481871/range2481871/1
component: BBa_K608007

https://synbiohub.org/public/igem/BBa_K1943001/annotation2481871/range2481871/1
type: Range
persistent identity: range2481871
display id: range2481871
version: 1
direction: direction/reverse
top level: BBa_K1943001
start: 1
end: 57
orientation: inline

BBa_K772103
type: ComponentDefinition
persistent identity: BBa K772103
display id: BBa_K772103
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: GFP - LacZ protein complex generator
creator: Mustafa Elitok
created: 2012-09-25T11:00:00Z
modified: 2015-05-08T01:13:15Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1024_
m user id: 0
owner id: 9573
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: GFP - LacZ protein generator is generated with the aim of inducing a reporting reaction aftermath of the activation of EpCAM detection system. (BBa_K772100) For more information: BBa_K772006
mutable notes: To achieve a constitutive production, pTetR promoter is added in the upstream region of the original part.
mutable provenance: Cleavage site, LacZ and GFP sequences are gathered from Registry.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K772006
component: BBa_R0040
sequence annotation: BBa_K772006
sequence annotation: BBa_R0040
sequence: https://synbiohub.org/public/igem/BBa_K772103_sequence/1

BBa_K772006
type: Component
persistent identity: component2211360
display id: component2211360
version: 1
top level: BBa_K772103
access: public
definition: AnRe

BBa_R0040
type: Component
persistent identity: component2211349
display id: component2211349
version: 1
top level: BBa_K772103
access: public
definition: https://synbiohub.org/public/igem/BBa_R0040/1

BBa_K772006
type: SequenceAnnotation
persistent identity: annotation2211360
display id: annotation2211360
version: 1
top level: BBa_K772103
location: https://synbiohub.org/public/igem/BBa_K772103/annotation2211360/range2211360/1
component: BBa_K772006

https://synbiohub.org/public/igem/BBa_K772103/annotation2211360/range2211360/1
type: Range
persistent identity: range2211360
display id: range2211360
version: 1
direction: direction/reverse
top level: BBa_K772103
start: 63
end: 1269
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2211349
display id: annotation2211349
version: 1
top level: BBa_K772103
location: https://synbiohub.org/public/igem/BBa_K772103/annotation2211349/range2211349/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_K772103/annotation2211349/range2211349/1
type: Range
persistent identity: range2211349
display id: range2211349
version: 1
direction: direction/forward
top level: BBa_K772103
start: 1
end: 54
orientation: inline

Tm-betaLac
type: ComponentDefinition
persistent identity: BBa K157019
display id: BBa_K157019
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Tm-betaLactamase(Bla2)
creator: iGEM Team Freiburg 2008
created: 2008-10-25T11:00:00Z
modified: 2015-05-08T01:10:54Z
discontinued: false
dominant: false
experience: experience/None
group u list: _232_
m user id: 0
owner id: 1673
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Second fragment of beta-Lactamase fused to the trans-membrane region of the Erbb1/EGF-receptor.
mutable notes: none
mutable provenance: Derived by cloning of the parts BBa_K157002, BBa_I757012. For further information see the description of the parts mentioned above.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: AgeI
sequence annotation: Tm-betaLactamase(Bla2)
sequence annotation: Tm
sequence annotation: Scar (Thr, Gly)
sequence annotation: Bla2
sequence annotation: NgoMI
sequence: https://synbiohub.org/public/igem/BBa_K157019_sequence/1

AgeI
type: SequenceAnnotation
persistent identity: annotation1989992
display id: annotation1989992
version: 1
top level: Tm-betaLac
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157019/annotation1989992/range1989992/1

https://synbiohub.org/public/igem/BBa_K157019/annotation1989992/range1989992/1
type: Range
persistent identity: range1989992
display id: range1989992
version: 1
direction: direction/forward
top level: Tm-betaLac
start: 355
end: 360
orientation: inline

Tm-betaLactamase(Bla2)
type: SequenceAnnotation
persistent identity: annotation1989993
display id: annotation1989993
version: 1
top level: Tm-betaLac
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K157019/annotation1989993/range1989993/1

https://synbiohub.org/public/igem/BBa_K157019/annotation1989993/range1989993/1
type: Range
persistent identity: range1989993
display id: range1989993
version: 1
direction: direction/forward
top level: Tm-betaLac
start: 1
end: 360
orientation: inline

Tm
type: SequenceAnnotation
persistent identity: annotation1991465
display id: annotation1991465
version: 1
top level: Tm-betaLac
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157019/annotation1991465/range1991465/1

https://synbiohub.org/public/igem/BBa_K157019/annotation1991465/range1991465/1
type: Range
persistent identity: range1991465
display id: range1991465
version: 1
direction: direction/forward
top level: Tm-betaLac
start: 10
end: 78
orientation: inline

Scar (Thr, Gly)
type: SequenceAnnotation
persistent identity: annotation1991466
display id: annotation1991466
version: 1
top level: Tm-betaLac
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157019/annotation1991466/range1991466/1

https://synbiohub.org/public/igem/BBa_K157019/annotation1991466/range1991466/1
type: Range
persistent identity: range1991466
display id: range1991466
version: 1
direction: direction/forward
top level: Tm-betaLac
start: 79
end: 84
orientation: inline

Bla2
type: SequenceAnnotation
persistent identity: annotation1991503
display id: annotation1991503
version: 1
top level: Tm-betaLac
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157019/annotation1991503/range1991503/1

https://synbiohub.org/public/igem/BBa_K157019/annotation1991503/range1991503/1
type: Range
persistent identity: range1991503
display id: range1991503
version: 1
direction: direction/forward
top level: Tm-betaLac
start: 85
end: 354
orientation: inline

NgoMI
type: SequenceAnnotation
persistent identity: annotation1989991
display id: annotation1989991
version: 1
top level: Tm-betaLac
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157019/annotation1989991/range1989991/1

https://synbiohub.org/public/igem/BBa_K157019/annotation1989991/range1989991/1
type: Range
persistent identity: range1989991
display id: range1989991
version: 1
direction: direction/forward
top level: Tm-betaLac
start: 4
end: 9
orientation: inline

LacZ
type: ComponentDefinition
persistent identity: BBa K1444017
display id: BBa_K1444017
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Full length beta-galactosidase (LacZ)
creator: Dan Ziemianowicz
created: 2014-10-08T11:00:00Z
modified: 2015-05-08T01:10:27Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1822_
m user id: 0
owner id: 20978
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: Beta-galactosidase (EC 3.2.1.23) is a classic enzyme most commonly used in blue-white colony screening when used in specialized vectors. This part consists of the full length beta-galactosidase sequence which can be inserted downstream of a promoter to produce the tetrameric, 47.4kDa enzyme. Beta-galactosidase cleaves the beta-glycosidic bond of a galactose bound to an organic moiety; the physiological function is to break down beta-galactosides, such as lactose, into it's respective monnosaccharides. Beta-galactosidase can act as a reporter when provided substrates such as X-gal. The cleavage of X-gal releases galactose and the chromophoric, subtsituted indole moiety. The most common form of X-gal is 5-Bromo-3-indolyl β-D-galactopyranoside, which produces an intense blue pigment. Other versions of X-gal may be used to produce other color pigments. Typically 40-60uL of a 20ug/mL X-gal solution, dissolved in dimethyl sulfoxide or dimethyl formamide, is spread on a plate and allowed to dry before plating the bacteria expressing beta-galactosidase. X-gal may also be added to liquid cultures or incorporated into solid agar media.
mutable notes: The addition of BioBrick prefix and suffix.
mutable provenance: Amplified and isolated from an expression vector.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence: https://synbiohub.org/public/igem/BBa_K1444017_sequence/1

MelA
type: ComponentDefinition
persistent identity: BBa K274001
display id: BBa_K274001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: melanin pigment
creator: Shuna Gould
created: 2009-08-02T11:00:00Z
modified: 2015-05-08T01:11:45Z
discontinued: false
dominant: false
experience: experience/None
group u list: _374_
m user id: 0
owner id: 5412
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Produces dark brown pigment output. The gene (melA) codes for a tyrosinase which produces the pigment from L-tyrosine. Production of the pigment requires the addition of copper and L-tyrosine supplements (the copper acts as a cofactor for the gene product) but no other precursors. The BioBrick sequence includes the native ribosome binding site.
mutable notes: The only changes to the sequence from the plasmid kindly provided by The Massachusetts Institute of Technology and Gregory Stephanopoulos was the removal of two resitrction sites, and the addition of the iGEM prefix and suffix. The two PstI restriction sites were removed by converting CAG to CAA in the second codon of the restriction site.
mutable provenance: The melA gene comes from the Rhizobium etli CFN 42 symbiotic plasmid p42d (accession number U80928). The origonal plasmid was created by Christine Nicole S. Santos and Gregory Stephanopoulos (reference: http://aem.asm.org/cgi/content/abstract/74/4/1190)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: MelA
sequence annotation: rbs
sequence annotation: stop
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_K274001_sequence/1

MelA
type: SequenceAnnotation
persistent identity: annotation2014738
display id: annotation2014738
version: 1
top level: MelA
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K274001/annotation2014738/range2014738/1

https://synbiohub.org/public/igem/BBa_K274001/annotation2014738/range2014738/1
type: Range
persistent identity: range2014738
display id: range2014738
version: 1
direction: direction/forward
top level: MelA
start: 15
end: 1844
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation2014737
display id: annotation2014737
version: 1
top level: MelA
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K274001/annotation2014737/range2014737/1

https://synbiohub.org/public/igem/BBa_K274001/annotation2014737/range2014737/1
type: Range
persistent identity: range2014737
display id: range2014737
version: 1
direction: direction/forward
top level: MelA
start: 1
end: 8
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2014757
display id: annotation2014757
version: 1
top level: MelA
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K274001/annotation2014757/range2014757/1

https://synbiohub.org/public/igem/BBa_K274001/annotation2014757/range2014757/1
type: Range
persistent identity: range2014757
display id: range2014757
version: 1
direction: direction/forward
top level: MelA
start: 1841
end: 1844
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2014756
display id: annotation2014756
version: 1
top level: MelA
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K274001/annotation2014756/range2014756/1

https://synbiohub.org/public/igem/BBa_K274001/annotation2014756/range2014756/1
type: Range
persistent identity: range2014756
display id: range2014756
version: 1
direction: direction/forward
top level: MelA
start: 15
end: 17
orientation: inline

BBa_K274002
type: ComponentDefinition
persistent identity: BBa K274002
display id: BBa_K274002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Vio operon
creator: Shuna Gould
created: 2009-10-07T11:00:00Z
modified: 2015-05-08T01:11:45Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _374_
m user id: 0
owner id: 5412
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: Produces a purple pigment (violacein) from L-tyrosine. The operon contains five genes (VioA-E) each with their own ribosome binding sites.
mutable notes: We added complementary restriction sites on either side of vioC and vioD (BamHI, BglII, HindIII). This allows them to be seperately removed to produce more colours from the operon.
mutable provenance: The VioA-E genes were originally from from Chromobacterium voilaceum ATCC 12472 in the pPSX vio+ plasmid. This was kindly provided by John Pemberton; Department of Microbiology and parasitology, University of Queensland, Brisbane, Australia. (Sarovich & Pemberton (2007) Plasmid 57:306-313). The codons were optimised for E. coli by DNA2.0 (Welch, Villalobos, Gustafsson and Minshull (2009) J. R. Soc. Interface)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: rbs
sequence annotation: vioA
sequence annotation: vioD
sequence annotation: vioC
sequence annotation: vioB
sequence annotation: vioE
sequence: https://synbiohub.org/public/igem/BBa_K274002_sequence/1

rbs
type: SequenceAnnotation
persistent identity: annotation2032720
display id: annotation2032720
version: 1
top level: BBa_K274002
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K274002/annotation2032720/range2032720/1

https://synbiohub.org/public/igem/BBa_K274002/annotation2032720/range2032720/1
type: Range
persistent identity: range2032720
display id: range2032720
version: 1
direction: direction/forward
top level: BBa_K274002
start: 1275
end: 1291
orientation: inline

vioA
type: SequenceAnnotation
persistent identity: annotation2032668
display id: annotation2032668
version: 1
top level: BBa_K274002
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K274002/annotation2032668/range2032668/1

https://synbiohub.org/public/igem/BBa_K274002/annotation2032668/range2032668/1
type: Range
persistent identity: range2032668
display id: range2032668
version: 1
direction: direction/forward
top level: BBa_K274002
start: 18
end: 1274
orientation: inline

vioD
type: SequenceAnnotation
persistent identity: annotation2032824
display id: annotation2032824
version: 1
top level: BBa_K274002
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K274002/annotation2032824/range2032824/1

https://synbiohub.org/public/igem/BBa_K274002/annotation2032824/range2032824/1
type: Range
persistent identity: range2032824
display id: range2032824
version: 1
direction: direction/forward
top level: BBa_K274002
start: 5625
end: 6752
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation2032821
display id: annotation2032821
version: 1
top level: BBa_K274002
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K274002/annotation2032821/range2032821/1

https://synbiohub.org/public/igem/BBa_K274002/annotation2032821/range2032821/1
type: Range
persistent identity: range2032821
display id: range2032821
version: 1
direction: direction/forward
top level: BBa_K274002
start: 4295
end: 4311
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation2032552
display id: annotation2032552
version: 1
top level: BBa_K274002
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K274002/annotation2032552/range2032552/1

https://synbiohub.org/public/igem/BBa_K274002/annotation2032552/range2032552/1
type: Range
persistent identity: range2032552
display id: range2032552
version: 1
direction: direction/forward
top level: BBa_K274002
start: 1
end: 17
orientation: inline

vioC
type: SequenceAnnotation
persistent identity: annotation2032822
display id: annotation2032822
version: 1
top level: BBa_K274002
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K274002/annotation2032822/range2032822/1

https://synbiohub.org/public/igem/BBa_K274002/annotation2032822/range2032822/1
type: Range
persistent identity: range2032822
display id: range2032822
version: 1
direction: direction/forward
top level: BBa_K274002
start: 4312
end: 5607
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation2032825
display id: annotation2032825
version: 1
top level: BBa_K274002
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K274002/annotation2032825/range2032825/1

https://synbiohub.org/public/igem/BBa_K274002/annotation2032825/range2032825/1
type: Range
persistent identity: range2032825
display id: range2032825
version: 1
direction: direction/forward
top level: BBa_K274002
start: 6753
end: 6769
orientation: inline

vioB
type: SequenceAnnotation
persistent identity: annotation2032721
display id: annotation2032721
version: 1
top level: BBa_K274002
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K274002/annotation2032721/range2032721/1

https://synbiohub.org/public/igem/BBa_K274002/annotation2032721/range2032721/1
type: Range
persistent identity: range2032721
display id: range2032721
version: 1
direction: direction/forward
top level: BBa_K274002
start: 1292
end: 4294
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation2032823
display id: annotation2032823
version: 1
top level: BBa_K274002
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K274002/annotation2032823/range2032823/1

https://synbiohub.org/public/igem/BBa_K274002/annotation2032823/range2032823/1
type: Range
persistent identity: range2032823
display id: range2032823
version: 1
direction: direction/forward
top level: BBa_K274002
start: 5608
end: 5624
orientation: inline

vioE
type: SequenceAnnotation
persistent identity: annotation2032826
display id: annotation2032826
version: 1
top level: BBa_K274002
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K274002/annotation2032826/range2032826/1

https://synbiohub.org/public/igem/BBa_K274002/annotation2032826/range2032826/1
type: Range
persistent identity: range2032826
display id: range2032826
version: 1
direction: direction/forward
top level: BBa_K274002
start: 6770
end: 7345
orientation: inline

SP-AntiNIP
type: ComponentDefinition
persistent identity: BBa K157025
display id: BBa_K157025
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: SP-AntiNIP-GGGSlinker-Tm-betaLactamase(Bla2)
creator: iGEM Team Freiburg 2008
created: 2008-10-25T11:00:00Z
modified: 2015-05-08T01:10:55Z
discontinued: false
dominant: false
experience: experience/None
group u list: _232_
m user id: 0
owner id: 1673
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: A composite part consisting of the signal peptide of Erbb1/EGF-receptor, scFV-antiNIP, GGGSflexlinker (extracellular part), the trans-membrane region of the Erbb1/EGF-receptor and the C-terminal part of the split-enzyme beta-Lactamase (intracellular part).
mutable notes: none
mutable provenance: Derived by cloning of the parts BBa_K157001, BBa_K157003, BBa_K157013, BBa_K157002 and BBa_I757012. For further information see the description of the parts mentioned above.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: Tm
sequence annotation: AgeI
sequence annotation: Sc-Fv_anti-NIP
sequence annotation: SP-AntiNIP-GGGSlinker-Tm-betaLactamase(Bla2)
sequence annotation: SP
sequence annotation: Scar (Thr, Gly)
sequence annotation: Bla2
sequence annotation: GGGS-Linker
sequence annotation: NgoMI
sequence: https://synbiohub.org/public/igem/BBa_K157025_sequence/1

Tm
type: SequenceAnnotation
persistent identity: annotation1992634
display id: annotation1992634
version: 1
top level: SP-AntiNIP
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157025/annotation1992634/range1992634/1

https://synbiohub.org/public/igem/BBa_K157025/annotation1992634/range1992634/1
type: Range
persistent identity: range1992634
display id: range1992634
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 880
end: 948
orientation: inline

AgeI
type: SequenceAnnotation
persistent identity: annotation1992637
display id: annotation1992637
version: 1
top level: SP-AntiNIP
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157025/annotation1992637/range1992637/1

https://synbiohub.org/public/igem/BBa_K157025/annotation1992637/range1992637/1
type: Range
persistent identity: range1992637
display id: range1992637
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 1225
end: 1230
orientation: inline

Sc-Fv_anti-NIP
type: SequenceAnnotation
persistent identity: annotation1992630
display id: annotation1992630
version: 1
top level: SP-AntiNIP
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157025/annotation1992630/range1992630/1

https://synbiohub.org/public/igem/BBa_K157025/annotation1992630/range1992630/1
type: Range
persistent identity: range1992630
display id: range1992630
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 88
end: 822
orientation: inline

SP-AntiNIP-GGGSlinker-Tm-betaLactamase(Bla2)
type: SequenceAnnotation
persistent identity: annotation1992626
display id: annotation1992626
version: 1
top level: SP-AntiNIP
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K157025/annotation1992626/range1992626/1

https://synbiohub.org/public/igem/BBa_K157025/annotation1992626/range1992626/1
type: Range
persistent identity: range1992626
display id: range1992626
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 1
end: 1230
orientation: inline

SP
type: SequenceAnnotation
persistent identity: annotation1992628
display id: annotation1992628
version: 1
top level: SP-AntiNIP
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157025/annotation1992628/range1992628/1

https://synbiohub.org/public/igem/BBa_K157025/annotation1992628/range1992628/1
type: Range
persistent identity: range1992628
display id: range1992628
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 10
end: 81
orientation: inline

Scar (Thr, Gly)
type: SequenceAnnotation
persistent identity: annotation1992629
display id: annotation1992629
version: 1
top level: SP-AntiNIP
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157025/annotation1992629/range1992629/1

https://synbiohub.org/public/igem/BBa_K157025/annotation1992629/range1992629/1
type: Range
persistent identity: range1992629
display id: range1992629
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 82
end: 87
orientation: inline

Bla2
type: SequenceAnnotation
persistent identity: annotation1992636
display id: annotation1992636
version: 1
top level: SP-AntiNIP
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157025/annotation1992636/range1992636/1

https://synbiohub.org/public/igem/BBa_K157025/annotation1992636/range1992636/1
type: Range
persistent identity: range1992636
display id: range1992636
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 955
end: 1224
orientation: inline

Scar (Thr, Gly)
type: SequenceAnnotation
persistent identity: annotation1992631
display id: annotation1992631
version: 1
top level: SP-AntiNIP
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157025/annotation1992631/range1992631/1

https://synbiohub.org/public/igem/BBa_K157025/annotation1992631/range1992631/1
type: Range
persistent identity: range1992631
display id: range1992631
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 823
end: 828
orientation: inline

Scar (Thr, Gly)
type: SequenceAnnotation
persistent identity: annotation1992635
display id: annotation1992635
version: 1
top level: SP-AntiNIP
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157025/annotation1992635/range1992635/1

https://synbiohub.org/public/igem/BBa_K157025/annotation1992635/range1992635/1
type: Range
persistent identity: range1992635
display id: range1992635
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 949
end: 954
orientation: inline

GGGS-Linker
type: SequenceAnnotation
persistent identity: annotation1992632
display id: annotation1992632
version: 1
top level: SP-AntiNIP
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157025/annotation1992632/range1992632/1

https://synbiohub.org/public/igem/BBa_K157025/annotation1992632/range1992632/1
type: Range
persistent identity: range1992632
display id: range1992632
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 829
end: 873
orientation: inline

NgoMI
type: SequenceAnnotation
persistent identity: annotation1992627
display id: annotation1992627
version: 1
top level: SP-AntiNIP
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157025/annotation1992627/range1992627/1

https://synbiohub.org/public/igem/BBa_K157025/annotation1992627/range1992627/1
type: Range
persistent identity: range1992627
display id: range1992627
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 4
end: 9
orientation: inline

Scar (Thr, Gly)
type: SequenceAnnotation
persistent identity: annotation1992633
display id: annotation1992633
version: 1
top level: SP-AntiNIP
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157025/annotation1992633/range1992633/1

https://synbiohub.org/public/igem/BBa_K157025/annotation1992633/range1992633/1
type: Range
persistent identity: range1992633
display id: range1992633
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 874
end: 879
orientation: inline

SP-AntiNIP
type: ComponentDefinition
persistent identity: BBa K157027
display id: BBa_K157027
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: SP-AntiNIP-GGGSlinker-Tm-nYFP
creator: iGEM Team Freiburg 2008
created: 2008-10-25T11:00:00Z
modified: 2015-05-08T01:10:55Z
discontinued: false
dominant: false
experience: experience/None
group u list: _232_
m user id: 0
owner id: 1673
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Part consists of the signal peptide of Erbb1/EGF-receptor, which ensures the transport to the cell membrane scFV-antiNIP, GGGSflexlinker (extracellular parts), the trans-membrane region of the Erbb1/EGF-receptor and the N-terminal part of the split-fluorophor YFP(intracellular part).
mutable notes: none
mutable provenance: Derived by cloning of the parts BBa_K157001, BBa_K157003, BBa_K157013, BBa_K157002, BBa_K157008. For further information see the description of the parts mentioned above.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: Scar (Thr, Gly)
sequence annotation: SP
sequence annotation: N-Term_YFP
sequence annotation: GGGS-Linker
sequence annotation: NgoMI
sequence annotation: SP-AntiNIP-GGGSlinker-Tm-nYFP
sequence annotation: Tm
sequence annotation: AgeI
sequence annotation: Sc-Fv_anti-NIP
sequence: https://synbiohub.org/public/igem/BBa_K157027_sequence/1

Scar (Thr, Gly)
type: SequenceAnnotation
persistent identity: annotation1992650
display id: annotation1992650
version: 1
top level: SP-AntiNIP
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157027/annotation1992650/range1992650/1

https://synbiohub.org/public/igem/BBa_K157027/annotation1992650/range1992650/1
type: Range
persistent identity: range1992650
display id: range1992650
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 82
end: 87
orientation: inline

Scar (Thr, Gly)
type: SequenceAnnotation
persistent identity: annotation1992658
display id: annotation1992658
version: 1
top level: SP-AntiNIP
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157027/annotation1992658/range1992658/1

https://synbiohub.org/public/igem/BBa_K157027/annotation1992658/range1992658/1
type: Range
persistent identity: range1992658
display id: range1992658
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 874
end: 879
orientation: inline

Scar (Thr, Gly)
type: SequenceAnnotation
persistent identity: annotation1992659
display id: annotation1992659
version: 1
top level: SP-AntiNIP
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157027/annotation1992659/range1992659/1

https://synbiohub.org/public/igem/BBa_K157027/annotation1992659/range1992659/1
type: Range
persistent identity: range1992659
display id: range1992659
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 949
end: 954
orientation: inline

SP
type: SequenceAnnotation
persistent identity: annotation1992649
display id: annotation1992649
version: 1
top level: SP-AntiNIP
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157027/annotation1992649/range1992649/1

https://synbiohub.org/public/igem/BBa_K157027/annotation1992649/range1992649/1
type: Range
persistent identity: range1992649
display id: range1992649
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 10
end: 81
orientation: inline

N-Term_YFP
type: SequenceAnnotation
persistent identity: annotation1992660
display id: annotation1992660
version: 1
top level: SP-AntiNIP
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157027/annotation1992660/range1992660/1

https://synbiohub.org/public/igem/BBa_K157027/annotation1992660/range1992660/1
type: Range
persistent identity: range1992660
display id: range1992660
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 955
end: 1422
orientation: inline

GGGS-Linker
type: SequenceAnnotation
persistent identity: annotation1992653
display id: annotation1992653
version: 1
top level: SP-AntiNIP
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157027/annotation1992653/range1992653/1

https://synbiohub.org/public/igem/BBa_K157027/annotation1992653/range1992653/1
type: Range
persistent identity: range1992653
display id: range1992653
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 829
end: 873
orientation: inline

NgoMI
type: SequenceAnnotation
persistent identity: annotation1990177
display id: annotation1990177
version: 1
top level: SP-AntiNIP
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157027/annotation1990177/range1990177/1

https://synbiohub.org/public/igem/BBa_K157027/annotation1990177/range1990177/1
type: Range
persistent identity: range1990177
display id: range1990177
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 4
end: 9
orientation: inline

SP-AntiNIP-GGGSlinker-Tm-nYFP
type: SequenceAnnotation
persistent identity: annotation1990179
display id: annotation1990179
version: 1
top level: SP-AntiNIP
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K157027/annotation1990179/range1990179/1

https://synbiohub.org/public/igem/BBa_K157027/annotation1990179/range1990179/1
type: Range
persistent identity: range1990179
display id: range1990179
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 1
end: 1428
orientation: inline

Tm
type: SequenceAnnotation
persistent identity: annotation1992655
display id: annotation1992655
version: 1
top level: SP-AntiNIP
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157027/annotation1992655/range1992655/1

https://synbiohub.org/public/igem/BBa_K157027/annotation1992655/range1992655/1
type: Range
persistent identity: range1992655
display id: range1992655
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 880
end: 948
orientation: inline

AgeI
type: SequenceAnnotation
persistent identity: annotation1990178
display id: annotation1990178
version: 1
top level: SP-AntiNIP
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157027/annotation1990178/range1990178/1

https://synbiohub.org/public/igem/BBa_K157027/annotation1990178/range1990178/1
type: Range
persistent identity: range1990178
display id: range1990178
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 1423
end: 1428
orientation: inline

Scar (Thr, Gly)
type: SequenceAnnotation
persistent identity: annotation1992652
display id: annotation1992652
version: 1
top level: SP-AntiNIP
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157027/annotation1992652/range1992652/1

https://synbiohub.org/public/igem/BBa_K157027/annotation1992652/range1992652/1
type: Range
persistent identity: range1992652
display id: range1992652
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 823
end: 828
orientation: inline

Sc-Fv_anti-NIP
type: SequenceAnnotation
persistent identity: annotation1992651
display id: annotation1992651
version: 1
top level: SP-AntiNIP
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157027/annotation1992651/range1992651/1

https://synbiohub.org/public/igem/BBa_K157027/annotation1992651/range1992651/1
type: Range
persistent identity: range1992651
display id: range1992651
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 88
end: 822
orientation: inline

LacZ
type: ComponentDefinition
persistent identity: BBa I732005
display id: BBa_I732005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: lacZ (encoding beta-galactosidase, full-length)
creator: Zhan Jian
created: 2007-07-05T11:00:00Z
modified: 2015-08-31T04:07:56Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: LacZ encodes beta-galactosidase, an intracellular enzyme that cleaves the disaccharide lactose into glucose and galactose. Beta-galactosidase can also cleaves X-gal and ONPG into colorful products.
mutable notes: None
mutable provenance: PCR amplified and standardized from BL21
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: C->T
sequence annotation: lacZ
sequence annotation: STOP
sequence annotation: START
sequence: https://synbiohub.org/public/igem/BBa_I732005_sequence/1

C->T
type: SequenceAnnotation
persistent identity: annotation1936869
display id: annotation1936869
version: 1
top level: LacZ
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_I732005/annotation1936869/range1936869/1

https://synbiohub.org/public/igem/BBa_I732005/annotation1936869/range1936869/1
type: Range
persistent identity: range1936869
display id: range1936869
version: 1
direction: direction/forward
top level: LacZ
start: 3024
end: 3024
orientation: inline

lacZ
type: SequenceAnnotation
persistent identity: annotation1936870
display id: annotation1936870
version: 1
top level: LacZ
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_I732005/annotation1936870/range1936870/1

https://synbiohub.org/public/igem/BBa_I732005/annotation1936870/range1936870/1
type: Range
persistent identity: range1936870
display id: range1936870
version: 1
direction: direction/forward
top level: LacZ
start: 1
end: 3075
orientation: inline

STOP
type: SequenceAnnotation
persistent identity: annotation1936872
display id: annotation1936872
version: 1
top level: LacZ
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_I732005/annotation1936872/range1936872/1

https://synbiohub.org/public/igem/BBa_I732005/annotation1936872/range1936872/1
type: Range
persistent identity: range1936872
display id: range1936872
version: 1
direction: direction/forward
top level: LacZ
start: 3073
end: 3075
orientation: inline

START
type: SequenceAnnotation
persistent identity: annotation1936871
display id: annotation1936871
version: 1
top level: LacZ
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_I732005/annotation1936871/range1936871/1

https://synbiohub.org/public/igem/BBa_I732005/annotation1936871/range1936871/1
type: Range
persistent identity: range1936871
display id: range1936871
version: 1
direction: direction/forward
top level: LacZ
start: 1
end: 3
orientation: inline

BBa_K1033904
type: ComponentDefinition
persistent identity: BBa K1033904
display id: BBa_K1033904
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: tsPurple, purple chromoprotein (incl RBS, J23106)
creator: Sabri Jamal
created: 2013-09-16T11:00:00Z
modified: 2015-05-08T01:08:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1340_
m user id: 0
owner id: 13997
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This chromoprotein naturally exhibits strong color when expressed.
mutable notes: Cloned from TinselPurple vector, DNA 2.0.
mutable provenance: Synthetic chromoprotein from DNA 2.0.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_J23106
component: BBa_K1033905
sequence annotation: BBa_J23106
sequence annotation: BBa_K1033905
sequence: https://synbiohub.org/public/igem/BBa_K1033904_sequence/1

BBa_J23106
type: Component
persistent identity: component2371208
display id: component2371208
version: 1
top level: BBa_K1033904
access: public
definition: https://synbiohub.org/public/igem/BBa_J23106/1

BBa_K1033905
type: Component
persistent identity: component2371212
display id: component2371212
version: 1
top level: BBa_K1033904
access: public
definition: https://synbiohub.org/public/igem/BBa_K1033905/1

BBa_J23106
type: SequenceAnnotation
persistent identity: annotation2371208
display id: annotation2371208
version: 1
top level: BBa_K1033904
location: https://synbiohub.org/public/igem/BBa_K1033904/annotation2371208/range2371208/1
component: BBa_J23106

https://synbiohub.org/public/igem/BBa_K1033904/annotation2371208/range2371208/1
type: Range
persistent identity: range2371208
display id: range2371208
version: 1
direction: direction/reverse
top level: BBa_K1033904
start: 1
end: 35
orientation: inline

BBa_K1033905
type: SequenceAnnotation
persistent identity: annotation2371212
display id: annotation2371212
version: 1
top level: BBa_K1033904
location: https://synbiohub.org/public/igem/BBa_K1033904/annotation2371212/range2371212/1
component: BBa_K1033905

https://synbiohub.org/public/igem/BBa_K1033904/annotation2371212/range2371212/1
type: Range
persistent identity: range2371212
display id: range2371212
version: 1
direction: direction/reverse
top level: BBa_K1033904
start: 44
end: 751
orientation: inline

BBa_K879222
type: ComponentDefinition
persistent identity: BBa K879222
display id: BBa_K879222
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Blue with promoter 2
creator: Nien-Tsu Tseng
created: 2012-09-30T11:00:00Z
modified: 2015-05-08T01:13:39Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1141_
m user id: 0
owner id: 13537
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: Blue with promoter 2
mutable notes: .
mutable provenance: .
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K879022
component: BBa_K879032
component: BBa_K879091
component: BBa_K879012
component: BBa_K879092
sequence annotation: BBa_K879092
sequence annotation: BBa_K879091
sequence annotation: BBa_K879032
sequence annotation: BBa_K879012
sequence annotation: BBa_K879022
sequence: https://synbiohub.org/public/igem/BBa_K879222_sequence/1

BBa_K879022
type: Component
persistent identity: component2205049
display id: component2205049
version: 1
top level: BBa_K879222
access: public
definition: https://synbiohub.org/public/igem/BBa_K879022/1

BBa_K879032
type: Component
persistent identity: component2205047
display id: component2205047
version: 1
top level: BBa_K879222
access: public
definition: https://synbiohub.org/public/igem/BBa_K879032/1

BBa_K879091
type: Component
persistent identity: component2205046
display id: component2205046
version: 1
top level: BBa_K879222
access: public
definition: https://synbiohub.org/public/igem/BBa_K879091/1

BBa_K879012
type: Component
persistent identity: component2205050
display id: component2205050
version: 1
top level: BBa_K879222
access: public
definition: https://synbiohub.org/public/igem/BBa_K879012/1

BBa_K879092
type: Component
persistent identity: component2205048
display id: component2205048
version: 1
top level: BBa_K879222
access: public
definition: https://synbiohub.org/public/igem/BBa_K879092/1

BBa_K879092
type: SequenceAnnotation
persistent identity: annotation2205048
display id: annotation2205048
version: 1
top level: BBa_K879222
location: https://synbiohub.org/public/igem/BBa_K879222/annotation2205048/range2205048/1
component: BBa_K879092

https://synbiohub.org/public/igem/BBa_K879222/annotation2205048/range2205048/1
type: Range
persistent identity: range2205048
display id: range2205048
version: 1
direction: direction/reverse
top level: BBa_K879222
start: 66
end: 74
orientation: inline

BBa_K879091
type: SequenceAnnotation
persistent identity: annotation2205046
display id: annotation2205046
version: 1
top level: BBa_K879222
location: https://synbiohub.org/public/igem/BBa_K879222/annotation2205046/range2205046/1
component: BBa_K879091

https://synbiohub.org/public/igem/BBa_K879222/annotation2205046/range2205046/1
type: Range
persistent identity: range2205046
display id: range2205046
version: 1
direction: direction/reverse
top level: BBa_K879222
start: 1
end: 36
orientation: inline

BBa_K879032
type: SequenceAnnotation
persistent identity: annotation2205047
display id: annotation2205047
version: 1
top level: BBa_K879222
location: https://synbiohub.org/public/igem/BBa_K879222/annotation2205047/range2205047/1
component: BBa_K879032

https://synbiohub.org/public/igem/BBa_K879222/annotation2205047/range2205047/1
type: Range
persistent identity: range2205047
display id: range2205047
version: 1
direction: direction/reverse
top level: BBa_K879222
start: 37
end: 65
orientation: inline

BBa_K879012
type: SequenceAnnotation
persistent identity: annotation2205050
display id: annotation2205050
version: 1
top level: BBa_K879222
location: https://synbiohub.org/public/igem/BBa_K879222/annotation2205050/range2205050/1
component: BBa_K879012

https://synbiohub.org/public/igem/BBa_K879222/annotation2205050/range2205050/1
type: Range
persistent identity: range2205050
display id: range2205050
version: 1
direction: direction/reverse
top level: BBa_K879222
start: 104
end: 772
orientation: inline

BBa_K879022
type: SequenceAnnotation
persistent identity: annotation2205049
display id: annotation2205049
version: 1
top level: BBa_K879222
location: https://synbiohub.org/public/igem/BBa_K879222/annotation2205049/range2205049/1
component: BBa_K879022

https://synbiohub.org/public/igem/BBa_K879222/annotation2205049/range2205049/1
type: Range
persistent identity: range2205049
display id: range2205049
version: 1
direction: direction/reverse
top level: BBa_K879222
start: 75
end: 103
orientation: inline

meffBlue
type: ComponentDefinition
persistent identity: BBa K1033901
display id: BBa_K1033901
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: meffBlue, blue chromoprotein (incl RBS)
creator: Erik Gullberg
created: 2013-09-16T11:00:00Z
modified: 2015-05-08T01:08:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1340_
m user id: 0
owner id: 13997
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This chromoprotein from the coral Montipora efflorescens, meffBlue, naturally exhibits strong color when expressed.
mutable notes: Synthesized and codon optimized for E coli by GenScript.
mutable provenance: Montipora efflorescens
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: BBa_K1033902
sequence annotation: rbs
sequence annotation: meffBlue
sequence: https://synbiohub.org/public/igem/BBa_K1033901_sequence/1

BBa_K1033902
type: SequenceAnnotation
persistent identity: annotation2349886
display id: annotation2349886
version: 1
top level: meffBlue
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1033901/annotation2349886/range2349886/1

https://synbiohub.org/public/igem/BBa_K1033901/annotation2349886/range2349886/1
type: Range
persistent identity: range2349886
display id: range2349886
version: 1
direction: direction/forward
top level: meffBlue
start: 40
end: 708
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation2349939
display id: annotation2349939
version: 1
top level: meffBlue
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1033901/annotation2349939/range2349939/1

https://synbiohub.org/public/igem/BBa_K1033901/annotation2349939/range2349939/1
type: Range
persistent identity: range2349939
display id: range2349939
version: 1
direction: direction/forward
top level: meffBlue
start: 22
end: 33
orientation: inline

meffBlue
type: SequenceAnnotation
persistent identity: annotation2349885
display id: annotation2349885
version: 1
top level: meffBlue
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1033901/annotation2349885/range2349885/1

https://synbiohub.org/public/igem/BBa_K1033901/annotation2349885/range2349885/1
type: Range
persistent identity: range2349885
display id: range2349885
version: 1
direction: direction/forward
top level: meffBlue
start: 40
end: 705
orientation: inline

BBa_K1033933
type: ComponentDefinition
persistent identity: BBa K1033933
display id: BBa_K1033933
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: asPink, pink chromoprotein
creator: Erik Lundin
created: 2014-04-20T11:00:00Z
modified: 2015-05-08T01:08:50Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1340_
m user id: 0
owner id: 9827
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: a
mutable notes: a
mutable provenance: a
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: asPink
sequence: https://synbiohub.org/public/igem/BBa_K1033933_sequence/1

asPink
type: SequenceAnnotation
persistent identity: annotation2379047
display id: annotation2379047
version: 1
top level: BBa_K1033933
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1033933/annotation2379047/range2379047/1

https://synbiohub.org/public/igem/BBa_K1033933/annotation2379047/range2379047/1
type: Range
persistent identity: range2379047
display id: range2379047
version: 1
direction: direction/forward
top level: BBa_K1033933
start: 1
end: 702
orientation: inline

BBa_K879313
type: ComponentDefinition
persistent identity: BBa K879313
display id: BBa_K879313
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Yellow with promoter 3
creator: Nien-Tsu Tseng
created: 2012-09-30T11:00:00Z
modified: 2015-05-08T01:13:39Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1141_
m user id: 0
owner id: 13537
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: Yellow with promoter 3
mutable notes: .
mutable provenance: .
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K879033
component: BBa_K879013
component: BBa_K879021
component: BBa_K879091
component: BBa_K879092
sequence annotation: BBa_K879033
sequence annotation: BBa_K879021
sequence annotation: BBa_K879013
sequence annotation: BBa_K879091
sequence annotation: BBa_K879092
sequence: https://synbiohub.org/public/igem/BBa_K879313_sequence/1

BBa_K879033
type: Component
persistent identity: component2205040
display id: component2205040
version: 1
top level: BBa_K879313
access: public
definition: https://synbiohub.org/public/igem/BBa_K879033/1

BBa_K879013
type: Component
persistent identity: component2205043
display id: component2205043
version: 1
top level: BBa_K879313
access: public
definition: https://synbiohub.org/public/igem/BBa_K879013/1

BBa_K879021
type: Component
persistent identity: component2205042
display id: component2205042
version: 1
top level: BBa_K879313
access: public
definition: https://synbiohub.org/public/igem/BBa_K879021/1

BBa_K879091
type: Component
persistent identity: component2205039
display id: component2205039
version: 1
top level: BBa_K879313
access: public
definition: https://synbiohub.org/public/igem/BBa_K879091/1

BBa_K879092
type: Component
persistent identity: component2205041
display id: component2205041
version: 1
top level: BBa_K879313
access: public
definition: https://synbiohub.org/public/igem/BBa_K879092/1

BBa_K879033
type: SequenceAnnotation
persistent identity: annotation2205040
display id: annotation2205040
version: 1
top level: BBa_K879313
location: https://synbiohub.org/public/igem/BBa_K879313/annotation2205040/range2205040/1
component: BBa_K879033

https://synbiohub.org/public/igem/BBa_K879313/annotation2205040/range2205040/1
type: Range
persistent identity: range2205040
display id: range2205040
version: 1
direction: direction/reverse
top level: BBa_K879313
start: 37
end: 65
orientation: inline

BBa_K879021
type: SequenceAnnotation
persistent identity: annotation2205042
display id: annotation2205042
version: 1
top level: BBa_K879313
location: https://synbiohub.org/public/igem/BBa_K879313/annotation2205042/range2205042/1
component: BBa_K879021

https://synbiohub.org/public/igem/BBa_K879313/annotation2205042/range2205042/1
type: Range
persistent identity: range2205042
display id: range2205042
version: 1
direction: direction/reverse
top level: BBa_K879313
start: 75
end: 100
orientation: inline

BBa_K879013
type: SequenceAnnotation
persistent identity: annotation2205043
display id: annotation2205043
version: 1
top level: BBa_K879313
location: https://synbiohub.org/public/igem/BBa_K879313/annotation2205043/range2205043/1
component: BBa_K879013

https://synbiohub.org/public/igem/BBa_K879313/annotation2205043/range2205043/1
type: Range
persistent identity: range2205043
display id: range2205043
version: 1
direction: direction/reverse
top level: BBa_K879313
start: 101
end: 799
orientation: inline

BBa_K879091
type: SequenceAnnotation
persistent identity: annotation2205039
display id: annotation2205039
version: 1
top level: BBa_K879313
location: https://synbiohub.org/public/igem/BBa_K879313/annotation2205039/range2205039/1
component: BBa_K879091

https://synbiohub.org/public/igem/BBa_K879313/annotation2205039/range2205039/1
type: Range
persistent identity: range2205039
display id: range2205039
version: 1
direction: direction/reverse
top level: BBa_K879313
start: 1
end: 36
orientation: inline

BBa_K879092
type: SequenceAnnotation
persistent identity: annotation2205041
display id: annotation2205041
version: 1
top level: BBa_K879313
location: https://synbiohub.org/public/igem/BBa_K879313/annotation2205041/range2205041/1
component: BBa_K879092

https://synbiohub.org/public/igem/BBa_K879313/annotation2205041/range2205041/1
type: Range
persistent identity: range2205041
display id: range2205041
version: 1
direction: direction/reverse
top level: BBa_K879313
start: 66
end: 74
orientation: inline

meffBlue
type: ComponentDefinition
persistent identity: BBa K1033902
display id: BBa_K1033902
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: meffBlue, blue chromoprotein
creator: Erik Gullberg
created: 2013-08-24T11:00:00Z
modified: 2015-05-08T01:08:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1340_
m user id: 0
owner id: 10137
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This chromoprotein from the coral Montipora efflorescens, meffBlue, naturally exhibits strong color when expressed.
mutable notes: Synthesized and codon optimized for E coli by GenScript.
mutable provenance: The coral Montipora efflorescens.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: meffBlue
sequence: https://synbiohub.org/public/igem/BBa_K1033902_sequence/1

meffBlue
type: SequenceAnnotation
persistent identity: annotation2332099
display id: annotation2332099
version: 1
top level: meffBlue
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1033902/annotation2332099/range2332099/1

https://synbiohub.org/public/igem/BBa_K1033902/annotation2332099/range2332099/1
type: Range
persistent identity: range2332099
display id: range2332099
version: 1
direction: direction/forward
top level: meffBlue
start: 1
end: 669
orientation: inline

BBa_K879311
type: ComponentDefinition
persistent identity: BBa K879311
display id: BBa_K879311
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Red with promoter 3
creator: Nien-Tsu Tseng
created: 2012-09-30T11:00:00Z
modified: 2015-05-08T01:13:39Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1141_
m user id: 0
owner id: 13537
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: Red with promoter 3
mutable notes: .
mutable provenance: .
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K879092
component: BBa_K879011
component: BBa_K879021
component: BBa_K879033
component: BBa_K879091
sequence annotation: BBa_K879011
sequence annotation: BBa_K879033
sequence annotation: BBa_K879021
sequence annotation: BBa_K879091
sequence annotation: BBa_K879092
sequence: https://synbiohub.org/public/igem/BBa_K879311_sequence/1

BBa_K879092
type: Component
persistent identity: component2205032
display id: component2205032
version: 1
top level: BBa_K879311
access: public
definition: https://synbiohub.org/public/igem/BBa_K879092/1

BBa_K879011
type: Component
persistent identity: component2205034
display id: component2205034
version: 1
top level: BBa_K879311
access: public
definition: https://synbiohub.org/public/igem/BBa_K879011/1

BBa_K879021
type: Component
persistent identity: component2205033
display id: component2205033
version: 1
top level: BBa_K879311
access: public
definition: https://synbiohub.org/public/igem/BBa_K879021/1

BBa_K879033
type: Component
persistent identity: component2205031
display id: component2205031
version: 1
top level: BBa_K879311
access: public
definition: https://synbiohub.org/public/igem/BBa_K879033/1

BBa_K879091
type: Component
persistent identity: component2205030
display id: component2205030
version: 1
top level: BBa_K879311
access: public
definition: https://synbiohub.org/public/igem/BBa_K879091/1

BBa_K879011
type: SequenceAnnotation
persistent identity: annotation2205034
display id: annotation2205034
version: 1
top level: BBa_K879311
location: https://synbiohub.org/public/igem/BBa_K879311/annotation2205034/range2205034/1
component: BBa_K879011

https://synbiohub.org/public/igem/BBa_K879311/annotation2205034/range2205034/1
type: Range
persistent identity: range2205034
display id: range2205034
version: 1
direction: direction/reverse
top level: BBa_K879311
start: 101
end: 781
orientation: inline

BBa_K879033
type: SequenceAnnotation
persistent identity: annotation2205031
display id: annotation2205031
version: 1
top level: BBa_K879311
location: https://synbiohub.org/public/igem/BBa_K879311/annotation2205031/range2205031/1
component: BBa_K879033

https://synbiohub.org/public/igem/BBa_K879311/annotation2205031/range2205031/1
type: Range
persistent identity: range2205031
display id: range2205031
version: 1
direction: direction/reverse
top level: BBa_K879311
start: 37
end: 65
orientation: inline

BBa_K879021
type: SequenceAnnotation
persistent identity: annotation2205033
display id: annotation2205033
version: 1
top level: BBa_K879311
location: https://synbiohub.org/public/igem/BBa_K879311/annotation2205033/range2205033/1
component: BBa_K879021

https://synbiohub.org/public/igem/BBa_K879311/annotation2205033/range2205033/1
type: Range
persistent identity: range2205033
display id: range2205033
version: 1
direction: direction/reverse
top level: BBa_K879311
start: 75
end: 100
orientation: inline

BBa_K879091
type: SequenceAnnotation
persistent identity: annotation2205030
display id: annotation2205030
version: 1
top level: BBa_K879311
location: https://synbiohub.org/public/igem/BBa_K879311/annotation2205030/range2205030/1
component: BBa_K879091

https://synbiohub.org/public/igem/BBa_K879311/annotation2205030/range2205030/1
type: Range
persistent identity: range2205030
display id: range2205030
version: 1
direction: direction/reverse
top level: BBa_K879311
start: 1
end: 36
orientation: inline

BBa_K879092
type: SequenceAnnotation
persistent identity: annotation2205032
display id: annotation2205032
version: 1
top level: BBa_K879311
location: https://synbiohub.org/public/igem/BBa_K879311/annotation2205032/range2205032/1
component: BBa_K879092

https://synbiohub.org/public/igem/BBa_K879311/annotation2205032/range2205032/1
type: Range
persistent identity: range2205032
display id: range2205032
version: 1
direction: direction/reverse
top level: BBa_K879311
start: 66
end: 74
orientation: inline

fwYellow
type: ComponentDefinition
persistent identity: BBa K1033909
display id: BBa_K1033909
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: fwYellow, yellow chromoprotein (incl RBS)
creator: Erik Gullberg
created: 2013-09-16T11:00:00Z
modified: 2015-05-08T01:08:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1340_
m user id: 0
owner id: 13997
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This chromoprotein naturally exhibits strong color when expressed.
mutable notes: Cloned from FezziwigYFP vector, DNA 2.0.
mutable provenance: Synthetic chromoprotein from DNA 2.0.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: BBa_K1033910
sequence annotation: fwYellow
sequence annotation: rbs
sequence: https://synbiohub.org/public/igem/BBa_K1033909_sequence/1

BBa_K1033910
type: SequenceAnnotation
persistent identity: annotation2350071
display id: annotation2350071
version: 1
top level: fwYellow
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1033909/annotation2350071/range2350071/1

https://synbiohub.org/public/igem/BBa_K1033909/annotation2350071/range2350071/1
type: Range
persistent identity: range2350071
display id: range2350071
version: 1
direction: direction/forward
top level: fwYellow
start: 19
end: 732
orientation: inline

fwYellow
type: SequenceAnnotation
persistent identity: annotation2350072
display id: annotation2350072
version: 1
top level: fwYellow
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1033909/annotation2350072/range2350072/1

https://synbiohub.org/public/igem/BBa_K1033909/annotation2350072/range2350072/1
type: Range
persistent identity: range2350072
display id: range2350072
version: 1
direction: direction/forward
top level: fwYellow
start: 19
end: 732
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation2350059
display id: annotation2350059
version: 1
top level: fwYellow
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1033909/annotation2350059/range2350059/1

https://synbiohub.org/public/igem/BBa_K1033909/annotation2350059/range2350059/1
type: Range
persistent identity: range2350059
display id: range2350059
version: 1
direction: direction/forward
top level: fwYellow
start: 1
end: 12
orientation: inline

BBa_K1468004
type: ComponentDefinition
persistent identity: BBa K1468004
display id: BBa_K1468004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pTet + gene encoding LacZ
creator: Pedro Luis Dorado Morales
created: 2014-10-02T11:00:00Z
modified: 2015-05-08T01:10:38Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1847_
m user id: 0
owner id: 11756
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: -
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: LacZ protein
sequence annotation: BBa_K1468004
sequence annotation: TetR 1
sequence annotation: BBa_I732005
sequence annotation: TetR 2
sequence annotation: LacZ
sequence annotation: -35
sequence annotation: BBa_R0040
sequence annotation: STOP
sequence annotation: C --> T
sequence annotation: -10
sequence annotation: START
sequence: https://synbiohub.org/public/igem/BBa_K1468004_sequence/1

LacZ protein
type: SequenceAnnotation
persistent identity: annotation2418063
display id: annotation2418063
version: 1
top level: BBa_K1468004
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1468004/annotation2418063/range2418063/1

https://synbiohub.org/public/igem/BBa_K1468004/annotation2418063/range2418063/1
type: Range
persistent identity: range2418063
display id: range2418063
version: 1
direction: direction/forward
top level: BBa_K1468004
start: 61
end: 3129
orientation: inline

BBa_K1468004
type: SequenceAnnotation
persistent identity: annotation2400601
display id: annotation2400601
version: 1
top level: BBa_K1468004
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1468004/annotation2400601/range2400601/1

https://synbiohub.org/public/igem/BBa_K1468004/annotation2400601/range2400601/1
type: Range
persistent identity: range2400601
display id: range2400601
version: 1
direction: direction/forward
top level: BBa_K1468004
start: 1
end: 3129
orientation: inline

TetR 1
type: SequenceAnnotation
persistent identity: annotation2400591
display id: annotation2400591
version: 1
top level: BBa_K1468004
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1468004/annotation2400591/range2400591/1

https://synbiohub.org/public/igem/BBa_K1468004/annotation2400591/range2400591/1
type: Range
persistent identity: range2400591
display id: range2400591
version: 1
direction: direction/forward
top level: BBa_K1468004
start: 1
end: 19
orientation: inline

BBa_I732005
type: SequenceAnnotation
persistent identity: annotation2400600
display id: annotation2400600
version: 1
top level: BBa_K1468004
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1468004/annotation2400600/range2400600/1

https://synbiohub.org/public/igem/BBa_K1468004/annotation2400600/range2400600/1
type: Range
persistent identity: range2400600
display id: range2400600
version: 1
direction: direction/forward
top level: BBa_K1468004
start: 61
end: 3129
orientation: inline

TetR 2
type: SequenceAnnotation
persistent identity: annotation2400594
display id: annotation2400594
version: 1
top level: BBa_K1468004
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1468004/annotation2400594/range2400594/1

https://synbiohub.org/public/igem/BBa_K1468004/annotation2400594/range2400594/1
type: Range
persistent identity: range2400594
display id: range2400594
version: 1
direction: direction/forward
top level: BBa_K1468004
start: 26
end: 44
orientation: inline

LacZ
type: SequenceAnnotation
persistent identity: annotation2400596
display id: annotation2400596
version: 1
top level: BBa_K1468004
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1468004/annotation2400596/range2400596/1

https://synbiohub.org/public/igem/BBa_K1468004/annotation2400596/range2400596/1
type: Range
persistent identity: range2400596
display id: range2400596
version: 1
direction: direction/forward
top level: BBa_K1468004
start: 61
end: 3129
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2400592
display id: annotation2400592
version: 1
top level: BBa_K1468004
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1468004/annotation2400592/range2400592/1

https://synbiohub.org/public/igem/BBa_K1468004/annotation2400592/range2400592/1
type: Range
persistent identity: range2400592
display id: range2400592
version: 1
direction: direction/forward
top level: BBa_K1468004
start: 20
end: 25
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2400593
display id: annotation2400593
version: 1
top level: BBa_K1468004
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1468004/annotation2400593/range2400593/1

https://synbiohub.org/public/igem/BBa_K1468004/annotation2400593/range2400593/1
type: Range
persistent identity: range2400593
display id: range2400593
version: 1
direction: direction/forward
top level: BBa_K1468004
start: 1
end: 54
orientation: inline

STOP
type: SequenceAnnotation
persistent identity: annotation2400599
display id: annotation2400599
version: 1
top level: BBa_K1468004
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1468004/annotation2400599/range2400599/1

https://synbiohub.org/public/igem/BBa_K1468004/annotation2400599/range2400599/1
type: Range
persistent identity: range2400599
display id: range2400599
version: 1
direction: direction/forward
top level: BBa_K1468004
start: 3129
end: 3129
orientation: inline

C --> T
type: SequenceAnnotation
persistent identity: annotation2400598
display id: annotation2400598
version: 1
top level: BBa_K1468004
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_K1468004/annotation2400598/range2400598/1

https://synbiohub.org/public/igem/BBa_K1468004/annotation2400598/range2400598/1
type: Range
persistent identity: range2400598
display id: range2400598
version: 1
direction: direction/forward
top level: BBa_K1468004
start: 3085
end: 3085
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2400595
display id: annotation2400595
version: 1
top level: BBa_K1468004
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1468004/annotation2400595/range2400595/1

https://synbiohub.org/public/igem/BBa_K1468004/annotation2400595/range2400595/1
type: Range
persistent identity: range2400595
display id: range2400595
version: 1
direction: direction/forward
top level: BBa_K1468004
start: 43
end: 48
orientation: inline

START
type: SequenceAnnotation
persistent identity: annotation2400597
display id: annotation2400597
version: 1
top level: BBa_K1468004
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1468004/annotation2400597/range2400597/1

https://synbiohub.org/public/igem/BBa_K1468004/annotation2400597/range2400597/1
type: Range
persistent identity: range2400597
display id: range2400597
version: 1
direction: direction/forward
top level: BBa_K1468004
start: 61
end: 63
orientation: inline

lacZ-alpha
type: ComponentDefinition
persistent identity: BBa I732006
display id: BBa_I732006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: lacZ alpha fragment
creator: Zhan Jian
created: 2007-07-06T11:00:00Z
modified: 2015-08-31T04:07:56Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: In strains with lacZ-omega (lacZ N-terminal deletion mutant) like DH5alpha, DH10B and Top10, lacZ-alpha restores the beta-galactosidase activity.
mutable notes: None
mutable provenance: PCR amplified from BBa_J33202 without the RBS.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: lacZ
sequence annotation: STOP
sequence annotation: START
sequence: https://synbiohub.org/public/igem/BBa_I732006_sequence/1

lacZ
type: SequenceAnnotation
persistent identity: annotation1936904
display id: annotation1936904
version: 1
top level: lacZ-alpha
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_I732006/annotation1936904/range1936904/1

https://synbiohub.org/public/igem/BBa_I732006/annotation1936904/range1936904/1
type: Range
persistent identity: range1936904
display id: range1936904
version: 1
direction: direction/forward
top level: lacZ-alpha
start: 1
end: 234
orientation: inline

STOP
type: SequenceAnnotation
persistent identity: annotation1936906
display id: annotation1936906
version: 1
top level: lacZ-alpha
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_I732006/annotation1936906/range1936906/1

https://synbiohub.org/public/igem/BBa_I732006/annotation1936906/range1936906/1
type: Range
persistent identity: range1936906
display id: range1936906
version: 1
direction: direction/forward
top level: lacZ-alpha
start: 229
end: 234
orientation: inline

START
type: SequenceAnnotation
persistent identity: annotation1936905
display id: annotation1936905
version: 1
top level: lacZ-alpha
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_I732006/annotation1936905/range1936905/1

https://synbiohub.org/public/igem/BBa_I732006/annotation1936905/range1936905/1
type: Range
persistent identity: range1936905
display id: range1936905
version: 1
direction: direction/forward
top level: lacZ-alpha
start: 1
end: 3
orientation: inline

BBa_K1033903
type: ComponentDefinition
persistent identity: BBa K1033903
display id: BBa_K1033903
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: tsPurple, purple chromoprotein (incl RBS, J23110)
creator: Sabri Jamal
created: 2013-09-16T11:00:00Z
modified: 2015-05-08T01:08:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1340_
m user id: 0
owner id: 13997
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This chromoprotein naturally exhibits strong color when expressed.
mutable notes: Cloned from TinselPurple vector, DNA 2.0.
mutable provenance: Synthetic chromoprotein from DNA 2.0.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_K1033905
component: BBa_J23110
sequence annotation: BBa_J23110
sequence annotation: BBa_K1033905
sequence: https://synbiohub.org/public/igem/BBa_K1033903_sequence/1

BBa_K1033905
type: Component
persistent identity: component2371217
display id: component2371217
version: 1
top level: BBa_K1033903
access: public
definition: https://synbiohub.org/public/igem/BBa_K1033905/1

BBa_J23110
type: Component
persistent identity: component2371213
display id: component2371213
version: 1
top level: BBa_K1033903
access: public
definition: https://synbiohub.org/public/igem/BBa_J23110/1

BBa_J23110
type: SequenceAnnotation
persistent identity: annotation2371213
display id: annotation2371213
version: 1
top level: BBa_K1033903
location: https://synbiohub.org/public/igem/BBa_K1033903/annotation2371213/range2371213/1
component: BBa_J23110

https://synbiohub.org/public/igem/BBa_K1033903/annotation2371213/range2371213/1
type: Range
persistent identity: range2371213
display id: range2371213
version: 1
direction: direction/reverse
top level: BBa_K1033903
start: 1
end: 35
orientation: inline

BBa_K1033905
type: SequenceAnnotation
persistent identity: annotation2371217
display id: annotation2371217
version: 1
top level: BBa_K1033903
location: https://synbiohub.org/public/igem/BBa_K1033903/annotation2371217/range2371217/1
component: BBa_K1033905

https://synbiohub.org/public/igem/BBa_K1033903/annotation2371217/range2371217/1
type: Range
persistent identity: range2371217
display id: range2371217
version: 1
direction: direction/reverse
top level: BBa_K1033903
start: 44
end: 751
orientation: inline

BBa_K592025
type: ComponentDefinition
persistent identity: BBa K592025
display id: BBa_K592025
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: B0034-amilCP
creator: Lei Sun
created: 2011-09-20T11:00:00Z
modified: 2015-05-08T01:12:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _763_
m user id: 0
owner id: 7929
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Blue chromoprotein amilCP (<partinfo>BBa_K592009</partinfo>) with B0034 RBS. One can add any promoter upstream of this gene.
mutable notes: amilCP was made BioBrick-compatible after removal of two illegal restriction sites.
mutable provenance: amilCP is from Acropora millepora
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_B0034
component: BBa_K592009
sequence annotation: BBa_B0034
sequence annotation: BBa_K592009
sequence: https://synbiohub.org/public/igem/BBa_K592025_sequence/1

BBa_B0034
type: Component
persistent identity: component2219031
display id: component2219031
version: 1
top level: BBa_K592025
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K592009
type: Component
persistent identity: component2219033
display id: component2219033
version: 1
top level: BBa_K592025
access: public
definition: https://synbiohub.org/public/igem/BBa_K592009/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2219031
display id: annotation2219031
version: 1
top level: BBa_K592025
location: https://synbiohub.org/public/igem/BBa_K592025/annotation2219031/range2219031/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K592025/annotation2219031/range2219031/1
type: Range
persistent identity: range2219031
display id: range2219031
version: 1
direction: direction/reverse
top level: BBa_K592025
start: 1
end: 12
orientation: inline

BBa_K592009
type: SequenceAnnotation
persistent identity: annotation2219033
display id: annotation2219033
version: 1
top level: BBa_K592025
location: https://synbiohub.org/public/igem/BBa_K592025/annotation2219033/range2219033/1
component: BBa_K592009

https://synbiohub.org/public/igem/BBa_K592025/annotation2219033/range2219033/1
type: Range
persistent identity: range2219033
display id: range2219033
version: 1
direction: direction/reverse
top level: BBa_K592025
start: 19
end: 687
orientation: inline

BBa_J04450
type: ComponentDefinition
persistent identity: BBa J04450
display id: BBa_J04450
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RFP Coding Device
creator: Tamar Odle
created: 2005-06-08T11:00:00Z
modified: 2015-08-31T04:08:14Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _16_
m user id: 0
owner id: 328
owning group id: 16
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Contains an IPTG inducible promoter an RBS, RFP, and terminator.
mutable provenance: Davidson Synth-Aces
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_B0034
component: BBa_E1010
component: BBa_R0010
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_R0010
sequence annotation: BBa_E1010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_J04450_sequence/1

BBa_B0034
type: Component
persistent identity: component1509404
display id: component1509404
version: 1
top level: BBa_J04450
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_E1010
type: Component
persistent identity: component1509411
display id: component1509411
version: 1
top level: BBa_J04450
access: public
definition: https://synbiohub.org/public/igem/BBa_E1010/1

BBa_R0010
type: Component
persistent identity: component1509394
display id: component1509394
version: 1
top level: BBa_J04450
access: public
definition: https://synbiohub.org/public/igem/BBa_R0010/1

BBa_B0012
type: Component
persistent identity: component1509427
display id: component1509427
version: 1
top level: BBa_J04450
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0010
type: Component
persistent identity: component1509417
display id: component1509417
version: 1
top level: BBa_J04450
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1509427
display id: annotation1509427
version: 1
top level: BBa_J04450
location: https://synbiohub.org/public/igem/BBa_J04450/annotation1509427/range1509427/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_J04450/annotation1509427/range1509427/1
type: Range
persistent identity: range1509427
display id: range1509427
version: 1
direction: direction/forward
top level: BBa_J04450
start: 1029
end: 1069
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation1509394
display id: annotation1509394
version: 1
top level: BBa_J04450
location: https://synbiohub.org/public/igem/BBa_J04450/annotation1509394/range1509394/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_J04450/annotation1509394/range1509394/1
type: Range
persistent identity: range1509394
display id: range1509394
version: 1
direction: direction/forward
top level: BBa_J04450
start: 1
end: 200
orientation: inline

BBa_E1010
type: SequenceAnnotation
persistent identity: annotation1509411
display id: annotation1509411
version: 1
top level: BBa_J04450
location: https://synbiohub.org/public/igem/BBa_J04450/annotation1509411/range1509411/1
component: BBa_E1010

https://synbiohub.org/public/igem/BBa_J04450/annotation1509411/range1509411/1
type: Range
persistent identity: range1509411
display id: range1509411
version: 1
direction: direction/forward
top level: BBa_J04450
start: 227
end: 907
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1509417
display id: annotation1509417
version: 1
top level: BBa_J04450
location: https://synbiohub.org/public/igem/BBa_J04450/annotation1509417/range1509417/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_J04450/annotation1509417/range1509417/1
type: Range
persistent identity: range1509417
display id: range1509417
version: 1
direction: direction/forward
top level: BBa_J04450
start: 941
end: 1020
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1509404
display id: annotation1509404
version: 1
top level: BBa_J04450
location: https://synbiohub.org/public/igem/BBa_J04450/annotation1509404/range1509404/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_J04450/annotation1509404/range1509404/1
type: Range
persistent identity: range1509404
display id: range1509404
version: 1
direction: direction/forward
top level: BBa_J04450
start: 209
end: 220
orientation: inline

BBa_J61032
type: ComponentDefinition
persistent identity: BBa J61032
display id: BBa_J61032
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Alkaline phosphatase [phoA]
creator: John Anderson
created: 2006-11-10T12:00:00Z
modified: 2015-08-31T02:02:59Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Alkaline phosphatase coding sequence (no rbs, promoter, or terminator present) from Citrobacter. This bacterium's phoA was chosen due simply to the absense of Biobrick restriction sites.
mutable notes: N/A
mutable provenance: pre-pcr ca1033F/ca1032R on Citrobacter gen (1500 bp) PCR ca1032F/R on above PCR (1500 bp, XbaI/EcoRI) Sub into pSB1A2-Bca1008 (XbaI/MfeI, 2077+935, large) Product is pSB1A2-Bca1032 ---- ca1032F Forward oligo to biobrick phoA of Citrobacter GGTTAtctagaTGTTTTTTATTTTAACTGTATTTATACATGGAG ca1032R Reverse oligo to biobrick phoA of Citrobacter cagtaGAATTCccagaggcggcaTTACTTCAG ca1033F Forward constant oligo to clone phoA ttatcatcgctccgcatcctg
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence: https://synbiohub.org/public/igem/BBa_J61032_sequence/1

SP-AntiNIP
type: ComponentDefinition
persistent identity: BBa K157029
display id: BBa_K157029
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: SP-AntiNIP-GGGSlinker-Tm-nCFP
creator: iGEM Team Freiburg 2008
created: 2008-10-25T11:00:00Z
modified: 2015-05-08T01:10:55Z
discontinued: false
dominant: false
experience: experience/None
group u list: _232_
m user id: 0
owner id: 1673
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Part consists of the signal peptide of Erbb1/EGF-receptor, scFV-antiNIP, GGGSflexlinker (extracellular part), the trans-membrane region of the Erbb1/EGF-receptor and the C-terminal part of the split fluorophor YFP with a fluolinker joined to its N-Terminus (intracellular part).
mutable notes: none
mutable provenance: Derived by cloning of the parts BBa_K157001, BBa_K157003, BBa_K157013, BBa_K157002, BBa_K157006. For further information see the description of the parts mentioned above.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: Tm
sequence annotation: Scar (Thr, Gly)
sequence annotation: SP
sequence annotation: N-Term_CFP
sequence annotation: GGGS-Linker
sequence annotation: Sc-Fv_anti-NIP
sequence annotation: SP-AntiNIP-GGGSlinker-Tm-nCFP
sequence annotation: AgeI
sequence annotation: NgoMI
sequence: https://synbiohub.org/public/igem/BBa_K157029_sequence/1

Tm
type: SequenceAnnotation
persistent identity: annotation1993306
display id: annotation1993306
version: 1
top level: SP-AntiNIP
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157029/annotation1993306/range1993306/1

https://synbiohub.org/public/igem/BBa_K157029/annotation1993306/range1993306/1
type: Range
persistent identity: range1993306
display id: range1993306
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 880
end: 948
orientation: inline

Scar (Thr, Gly)
type: SequenceAnnotation
persistent identity: annotation1993301
display id: annotation1993301
version: 1
top level: SP-AntiNIP
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157029/annotation1993301/range1993301/1

https://synbiohub.org/public/igem/BBa_K157029/annotation1993301/range1993301/1
type: Range
persistent identity: range1993301
display id: range1993301
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 82
end: 87
orientation: inline

Scar (Thr, Gly)
type: SequenceAnnotation
persistent identity: annotation1993303
display id: annotation1993303
version: 1
top level: SP-AntiNIP
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157029/annotation1993303/range1993303/1

https://synbiohub.org/public/igem/BBa_K157029/annotation1993303/range1993303/1
type: Range
persistent identity: range1993303
display id: range1993303
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 823
end: 828
orientation: inline

Scar (Thr, Gly)
type: SequenceAnnotation
persistent identity: annotation1993307
display id: annotation1993307
version: 1
top level: SP-AntiNIP
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157029/annotation1993307/range1993307/1

https://synbiohub.org/public/igem/BBa_K157029/annotation1993307/range1993307/1
type: Range
persistent identity: range1993307
display id: range1993307
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 949
end: 954
orientation: inline

SP
type: SequenceAnnotation
persistent identity: annotation1993300
display id: annotation1993300
version: 1
top level: SP-AntiNIP
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157029/annotation1993300/range1993300/1

https://synbiohub.org/public/igem/BBa_K157029/annotation1993300/range1993300/1
type: Range
persistent identity: range1993300
display id: range1993300
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 10
end: 81
orientation: inline

N-Term_CFP
type: SequenceAnnotation
persistent identity: annotation1993308
display id: annotation1993308
version: 1
top level: SP-AntiNIP
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157029/annotation1993308/range1993308/1

https://synbiohub.org/public/igem/BBa_K157029/annotation1993308/range1993308/1
type: Range
persistent identity: range1993308
display id: range1993308
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 955
end: 1419
orientation: inline

GGGS-Linker
type: SequenceAnnotation
persistent identity: annotation1993304
display id: annotation1993304
version: 1
top level: SP-AntiNIP
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157029/annotation1993304/range1993304/1

https://synbiohub.org/public/igem/BBa_K157029/annotation1993304/range1993304/1
type: Range
persistent identity: range1993304
display id: range1993304
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 829
end: 873
orientation: inline

Sc-Fv_anti-NIP
type: SequenceAnnotation
persistent identity: annotation1993302
display id: annotation1993302
version: 1
top level: SP-AntiNIP
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157029/annotation1993302/range1993302/1

https://synbiohub.org/public/igem/BBa_K157029/annotation1993302/range1993302/1
type: Range
persistent identity: range1993302
display id: range1993302
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 88
end: 822
orientation: inline

SP-AntiNIP-GGGSlinker-Tm-nCFP
type: SequenceAnnotation
persistent identity: annotation1990253
display id: annotation1990253
version: 1
top level: SP-AntiNIP
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K157029/annotation1990253/range1990253/1

https://synbiohub.org/public/igem/BBa_K157029/annotation1990253/range1990253/1
type: Range
persistent identity: range1990253
display id: range1990253
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 1
end: 1425
orientation: inline

AgeI
type: SequenceAnnotation
persistent identity: annotation1990245
display id: annotation1990245
version: 1
top level: SP-AntiNIP
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157029/annotation1990245/range1990245/1

https://synbiohub.org/public/igem/BBa_K157029/annotation1990245/range1990245/1
type: Range
persistent identity: range1990245
display id: range1990245
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 1420
end: 1425
orientation: inline

NgoMI
type: SequenceAnnotation
persistent identity: annotation1990244
display id: annotation1990244
version: 1
top level: SP-AntiNIP
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157029/annotation1990244/range1990244/1

https://synbiohub.org/public/igem/BBa_K157029/annotation1990244/range1990244/1
type: Range
persistent identity: range1990244
display id: range1990244
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 4
end: 9
orientation: inline

Scar (Thr, Gly)
type: SequenceAnnotation
persistent identity: annotation1993305
display id: annotation1993305
version: 1
top level: SP-AntiNIP
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157029/annotation1993305/range1993305/1

https://synbiohub.org/public/igem/BBa_K157029/annotation1993305/range1993305/1
type: Range
persistent identity: range1993305
display id: range1993305
version: 1
direction: direction/forward
top level: SP-AntiNIP
start: 874
end: 879
orientation: inline

aeBlue
type: ComponentDefinition
persistent identity: BBa K864403
display id: BBa_K864403
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: J23110-B0034-aeBlue (chromoprotein) (E.coli optimized)
creator: Olle Nordesj??
created: 2012-09-24T11:00:00Z
modified: 2015-05-08T01:13:37Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1124_
m user id: 0
owner id: 14206
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: aeBlue is a blue chromoprotein extracted from the basal disk of a beadlet anemone Actinia equina. The protein has an absorption maximum at 597nm and a deep blue colour. The protein aeBlue is of the GFP family with significant sequence similarities. This part is a functionally active variant of BBa_K864401.
mutable notes: The promoter J23110 is sufficiently strong to yield expression levels making the blue protein clearly visible and eventually opaque.
mutable provenance: The protein was discovered, extracted and analyzed in (Shkrob, 2005). It was synthesized by Bioneer Corporation, codon optimized for E.coli.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_J23110
component: BBa_K864401
component: BBa_B0034
sequence annotation: BBa_J23110
sequence annotation: BBa_K864401
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K864403_sequence/1

BBa_J23110
type: Component
persistent identity: component2198623
display id: component2198623
version: 1
top level: aeBlue
access: public
definition: https://synbiohub.org/public/igem/BBa_J23110/1

BBa_K864401
type: Component
persistent identity: component2198627
display id: component2198627
version: 1
top level: aeBlue
access: public
definition: https://synbiohub.org/public/igem/BBa_K864401/1

BBa_B0034
type: Component
persistent identity: component2198625
display id: component2198625
version: 1
top level: aeBlue
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_J23110
type: SequenceAnnotation
persistent identity: annotation2198623
display id: annotation2198623
version: 1
top level: aeBlue
location: https://synbiohub.org/public/igem/BBa_K864403/annotation2198623/range2198623/1
component: BBa_J23110

https://synbiohub.org/public/igem/BBa_K864403/annotation2198623/range2198623/1
type: Range
persistent identity: range2198623
display id: range2198623
version: 1
direction: direction/reverse
top level: aeBlue
start: 1
end: 35
orientation: inline

BBa_K864401
type: SequenceAnnotation
persistent identity: annotation2198627
display id: annotation2198627
version: 1
top level: aeBlue
location: https://synbiohub.org/public/igem/BBa_K864403/annotation2198627/range2198627/1
component: BBa_K864401

https://synbiohub.org/public/igem/BBa_K864403/annotation2198627/range2198627/1
type: Range
persistent identity: range2198627
display id: range2198627
version: 1
direction: direction/reverse
top level: aeBlue
start: 62
end: 760
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2198625
display id: annotation2198625
version: 1
top level: aeBlue
location: https://synbiohub.org/public/igem/BBa_K864403/annotation2198625/range2198625/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K864403/annotation2198625/range2198625/1
type: Range
persistent identity: range2198625
display id: range2198625
version: 1
direction: direction/reverse
top level: aeBlue
start: 44
end: 55
orientation: inline

AnRe
type: ComponentDefinition
persistent identity: BBa K772006
display id: BBa_K772006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: GFP - LacZ anchor-inducer protein complex
creator: Mustafa Elitok
created: 2012-09-24T11:00:00Z
modified: 2015-05-08T01:13:15Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1024_
m user id: 0
owner id: 9573
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This part contains a green fluorescent protein (GFP) as a non-reactive chemical compound (anchor) in order to neutralize the active reactive fixed to it, a reporter/inducer device or protein (enzyme etc.) and a TEV protease cleavage site between them to assemble these two proteins. BBa_772001 and BBa_K772002 are assembled to be used as the trigger of detection in the whole system which ends with the intracellular transfection (or activation) of TEV protease. After the activation, it is planned that TEV protease will cleave this anchor-inducer protein complex to release free the inducer/reporter. In conclusion, the inducer would activate a transcription pathway on the targeted inducible gene or the reporter would indicate that there is a change in the whole system.
mutable notes: Note that there is an RBS sequence just before the protein sequence in order to eliminate the need of addition of it.
mutable provenance: Cleavage site, LacZ and GFP sequences are gathered from Registry.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
sequence annotation: GFP
sequence annotation: Double Terminator
sequence annotation: Anchor - Reporter
sequence annotation: LacZ
sequence annotation: RBS
sequence annotation: TEV cleavage site
sequence: https://synbiohub.org/public/igem/BBa_K772006_sequence/1

GFP
type: SequenceAnnotation
persistent identity: annotation2203904
display id: annotation2203904
version: 1
top level: AnRe
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K772006/annotation2203904/range2203904/1

https://synbiohub.org/public/igem/BBa_K772006/annotation2203904/range2203904/1
type: Range
persistent identity: range2203904
display id: range2203904
version: 1
direction: direction/forward
top level: AnRe
start: 21
end: 741
orientation: inline

Double Terminator
type: SequenceAnnotation
persistent identity: annotation2203907
display id: annotation2203907
version: 1
top level: AnRe
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K772006/annotation2203907/range2203907/1

https://synbiohub.org/public/igem/BBa_K772006/annotation2203907/range2203907/1
type: Range
persistent identity: range2203907
display id: range2203907
version: 1
direction: direction/forward
top level: AnRe
start: 1076
end: 1207
orientation: inline

Anchor - Reporter
type: SequenceAnnotation
persistent identity: annotation2203908
display id: annotation2203908
version: 1
top level: AnRe
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K772006/annotation2203908/range2203908/1

https://synbiohub.org/public/igem/BBa_K772006/annotation2203908/range2203908/1
type: Range
persistent identity: range2203908
display id: range2203908
version: 1
direction: direction/forward
top level: AnRe
start: 21
end: 1075
orientation: inline

LacZ
type: SequenceAnnotation
persistent identity: annotation2203906
display id: annotation2203906
version: 1
top level: AnRe
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K772006/annotation2203906/range2203906/1

https://synbiohub.org/public/igem/BBa_K772006/annotation2203906/range2203906/1
type: Range
persistent identity: range2203906
display id: range2203906
version: 1
direction: direction/forward
top level: AnRe
start: 832
end: 1075
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2203903
display id: annotation2203903
version: 1
top level: AnRe
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K772006/annotation2203903/range2203903/1

https://synbiohub.org/public/igem/BBa_K772006/annotation2203903/range2203903/1
type: Range
persistent identity: range2203903
display id: range2203903
version: 1
direction: direction/forward
top level: AnRe
start: 1
end: 20
orientation: inline

TEV cleavage site
type: SequenceAnnotation
persistent identity: annotation2203905
display id: annotation2203905
version: 1
top level: AnRe
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K772006/annotation2203905/range2203905/1

https://synbiohub.org/public/igem/BBa_K772006/annotation2203905/range2203905/1
type: Range
persistent identity: range2203905
display id: range2203905
version: 1
direction: direction/forward
top level: AnRe
start: 742
end: 831
orientation: inline

SP-Lipocal
type: ComponentDefinition
persistent identity: BBa K157033
display id: BBa_K157033
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: SP-Lipocalin-GGGSlinker-Tm-betaLactamase(Bla2)
creator: iGEM Team Freiburg 2008
created: 2008-10-25T11:00:00Z
modified: 2015-05-08T01:10:55Z
discontinued: false
dominant: false
experience: experience/None
group u list: _232_
m user id: 0
owner id: 1673
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Part consists of the signal peptide of Erbb1/EGF-receptor, scFV-antiNIP, GGGSflexlinker (extracellular part), the trans-membrane region of the Erbb1/EGF-receptor and the C-terminal part of the split enzyme beta-Lactamase (intracellular part).
mutable notes: none
mutable provenance: Derived by cloning of the parts BBa_K157001, BBa_K157004, BBa_K157013, BBa_K157002 and BBa_I757012. For further information see the description of the parts mentioned above.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: Scar (Thr, Gly)
sequence annotation: SP
sequence annotation: Lipocalin
sequence annotation: Tm
sequence annotation: NgoMI
sequence annotation: GGGS-Linker
sequence annotation: Bla2
sequence annotation: SP-Lipocalin-GGGSlinker-Tm-betaLactamase(Bla2)
sequence annotation: AgeI
sequence: https://synbiohub.org/public/igem/BBa_K157033_sequence/1

Scar (Thr, Gly)
type: SequenceAnnotation
persistent identity: annotation1993344
display id: annotation1993344
version: 1
top level: SP-Lipocal
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157033/annotation1993344/range1993344/1

https://synbiohub.org/public/igem/BBa_K157033/annotation1993344/range1993344/1
type: Range
persistent identity: range1993344
display id: range1993344
version: 1
direction: direction/forward
top level: SP-Lipocal
start: 610
end: 615
orientation: inline

SP
type: SequenceAnnotation
persistent identity: annotation1993341
display id: annotation1993341
version: 1
top level: SP-Lipocal
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157033/annotation1993341/range1993341/1

https://synbiohub.org/public/igem/BBa_K157033/annotation1993341/range1993341/1
type: Range
persistent identity: range1993341
display id: range1993341
version: 1
direction: direction/forward
top level: SP-Lipocal
start: 10
end: 81
orientation: inline

Lipocalin
type: SequenceAnnotation
persistent identity: annotation1993343
display id: annotation1993343
version: 1
top level: SP-Lipocal
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157033/annotation1993343/range1993343/1

https://synbiohub.org/public/igem/BBa_K157033/annotation1993343/range1993343/1
type: Range
persistent identity: range1993343
display id: range1993343
version: 1
direction: direction/forward
top level: SP-Lipocal
start: 88
end: 609
orientation: inline

Scar (Thr, Gly)
type: SequenceAnnotation
persistent identity: annotation1993342
display id: annotation1993342
version: 1
top level: SP-Lipocal
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157033/annotation1993342/range1993342/1

https://synbiohub.org/public/igem/BBa_K157033/annotation1993342/range1993342/1
type: Range
persistent identity: range1993342
display id: range1993342
version: 1
direction: direction/forward
top level: SP-Lipocal
start: 82
end: 87
orientation: inline

Tm
type: SequenceAnnotation
persistent identity: annotation1993347
display id: annotation1993347
version: 1
top level: SP-Lipocal
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157033/annotation1993347/range1993347/1

https://synbiohub.org/public/igem/BBa_K157033/annotation1993347/range1993347/1
type: Range
persistent identity: range1993347
display id: range1993347
version: 1
direction: direction/forward
top level: SP-Lipocal
start: 667
end: 735
orientation: inline

NgoMI
type: SequenceAnnotation
persistent identity: annotation1990272
display id: annotation1990272
version: 1
top level: SP-Lipocal
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157033/annotation1990272/range1990272/1

https://synbiohub.org/public/igem/BBa_K157033/annotation1990272/range1990272/1
type: Range
persistent identity: range1990272
display id: range1990272
version: 1
direction: direction/forward
top level: SP-Lipocal
start: 4
end: 9
orientation: inline

GGGS-Linker
type: SequenceAnnotation
persistent identity: annotation1993345
display id: annotation1993345
version: 1
top level: SP-Lipocal
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157033/annotation1993345/range1993345/1

https://synbiohub.org/public/igem/BBa_K157033/annotation1993345/range1993345/1
type: Range
persistent identity: range1993345
display id: range1993345
version: 1
direction: direction/forward
top level: SP-Lipocal
start: 616
end: 660
orientation: inline

Bla2
type: SequenceAnnotation
persistent identity: annotation1993349
display id: annotation1993349
version: 1
top level: SP-Lipocal
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157033/annotation1993349/range1993349/1

https://synbiohub.org/public/igem/BBa_K157033/annotation1993349/range1993349/1
type: Range
persistent identity: range1993349
display id: range1993349
version: 1
direction: direction/forward
top level: SP-Lipocal
start: 742
end: 1011
orientation: inline

SP-Lipocalin-GGGSlinker-Tm-betaLactamase(Bla2)
type: SequenceAnnotation
persistent identity: annotation1990274
display id: annotation1990274
version: 1
top level: SP-Lipocal
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K157033/annotation1990274/range1990274/1

https://synbiohub.org/public/igem/BBa_K157033/annotation1990274/range1990274/1
type: Range
persistent identity: range1990274
display id: range1990274
version: 1
direction: direction/forward
top level: SP-Lipocal
start: 1
end: 1017
orientation: inline

Scar (Thr, Gly)
type: SequenceAnnotation
persistent identity: annotation1993348
display id: annotation1993348
version: 1
top level: SP-Lipocal
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157033/annotation1993348/range1993348/1

https://synbiohub.org/public/igem/BBa_K157033/annotation1993348/range1993348/1
type: Range
persistent identity: range1993348
display id: range1993348
version: 1
direction: direction/forward
top level: SP-Lipocal
start: 736
end: 741
orientation: inline

AgeI
type: SequenceAnnotation
persistent identity: annotation1990273
display id: annotation1990273
version: 1
top level: SP-Lipocal
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157033/annotation1990273/range1990273/1

https://synbiohub.org/public/igem/BBa_K157033/annotation1990273/range1990273/1
type: Range
persistent identity: range1990273
display id: range1990273
version: 1
direction: direction/forward
top level: SP-Lipocal
start: 1012
end: 1017
orientation: inline

Scar (Thr, Gly)
type: SequenceAnnotation
persistent identity: annotation1993346
display id: annotation1993346
version: 1
top level: SP-Lipocal
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157033/annotation1993346/range1993346/1

https://synbiohub.org/public/igem/BBa_K157033/annotation1993346/range1993346/1
type: Range
persistent identity: range1993346
display id: range1993346
version: 1
direction: direction/forward
top level: SP-Lipocal
start: 661
end: 666
orientation: inline

LacZ
type: ComponentDefinition
persistent identity: BBa K678026
display id: BBa_K678026
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LacZ for Plug
creator: DTU-Denmark-2
created: 2011-09-06T11:00:00Z
modified: 2015-05-08T01:13:04Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _882_
m user id: 0
owner id: 8341
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: LacZ functional in Aspergillus nidulans
mutable notes: Compatible with the Plug'n'Play with DNA assembly standard
mutable provenance: p68
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: lacZ
sequence annotation: Linker 2
sequence: https://synbiohub.org/public/igem/BBa_K678026_sequence/1

lacZ
type: SequenceAnnotation
persistent identity: annotation2127381
display id: annotation2127381
version: 1
top level: LacZ
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K678026/annotation2127381/range2127381/1

https://synbiohub.org/public/igem/BBa_K678026/annotation2127381/range2127381/1
type: Range
persistent identity: range2127381
display id: range2127381
version: 1
direction: direction/forward
top level: LacZ
start: 9
end: 3083
orientation: inline

Linker 2
type: SequenceAnnotation
persistent identity: annotation2127380
display id: annotation2127380
version: 1
top level: LacZ
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K678026/annotation2127380/range2127380/1

https://synbiohub.org/public/igem/BBa_K678026/annotation2127380/range2127380/1
type: Range
persistent identity: range2127380
display id: range2127380
version: 1
direction: direction/forward
top level: LacZ
start: 1
end: 8
orientation: inline

BBa_K1033900
type: ComponentDefinition
persistent identity: BBa K1033900
display id: BBa_K1033900
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: meffBlue, blue chromoprotein (incl RBS, J23110)
creator: Sabri Jamal
created: 2013-09-16T11:00:00Z
modified: 2015-05-08T01:08:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1340_
m user id: 0
owner id: 13997
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This chromoprotein from the coral Montipora efflorescens, meffBlue, naturally exhibits strong color when expressed.
mutable notes: Synthesized and codon optimized for E coli by GenScript.
mutable provenance: Montipora efflorescens
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_K1033901
component: BBa_J23110
sequence annotation: BBa_K1033901
sequence annotation: BBa_J23110
sequence: https://synbiohub.org/public/igem/BBa_K1033900_sequence/1

BBa_K1033901
type: Component
persistent identity: component2371207
display id: component2371207
version: 1
top level: BBa_K1033900
access: public
definition: meffBlue

BBa_J23110
type: Component
persistent identity: component2371203
display id: component2371203
version: 1
top level: BBa_K1033900
access: public
definition: https://synbiohub.org/public/igem/BBa_J23110/1

BBa_K1033901
type: SequenceAnnotation
persistent identity: annotation2371207
display id: annotation2371207
version: 1
top level: BBa_K1033900
location: https://synbiohub.org/public/igem/BBa_K1033900/annotation2371207/range2371207/1
component: BBa_K1033901

https://synbiohub.org/public/igem/BBa_K1033900/annotation2371207/range2371207/1
type: Range
persistent identity: range2371207
display id: range2371207
version: 1
direction: direction/reverse
top level: BBa_K1033900
start: 44
end: 751
orientation: inline

BBa_J23110
type: SequenceAnnotation
persistent identity: annotation2371203
display id: annotation2371203
version: 1
top level: BBa_K1033900
location: https://synbiohub.org/public/igem/BBa_K1033900/annotation2371203/range2371203/1
component: BBa_J23110

https://synbiohub.org/public/igem/BBa_K1033900/annotation2371203/range2371203/1
type: Range
persistent identity: range2371203
display id: range2371203
version: 1
direction: direction/reverse
top level: BBa_K1033900
start: 1
end: 35
orientation: inline

sfGFP(Sp)
type: ComponentDefinition
persistent identity: BBa K1930006
display id: BBa_K1930006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: sfGFP(Sp)
creator: Barbora Waclawikova
created: 2016-10-15T11:00:00Z
modified: 2016-10-16T05:16:06Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2397_
m user id: 29748
owner id: 29748
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This part is a reporter sfGFP(Sp) originally optimized for Streptococcus pneumoniae.
mutable notes: sfGFP originally optimized for streptococcus pneumonia because it could be shown that the signal in Bacillus is even brighter than the one from sfGFP(Bs).
mutable provenance: Plasmid pNW
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: sfGFP(Sp)
sequence: https://synbiohub.org/public/igem/BBa_K1930006_sequence/1

sfGFP(Sp)
type: SequenceAnnotation
persistent identity: annotation2513522
display id: annotation2513522
version: 1
top level: sfGFP(Sp)
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1930006/annotation2513522/range2513522/1

https://synbiohub.org/public/igem/BBa_K1930006/annotation2513522/range2513522/1
type: Range
persistent identity: range2513522
display id: range2513522
version: 1
direction: direction/forward
top level: sfGFP(Sp)
start: 1
end: 722
orientation: inline

asPink
type: ComponentDefinition
persistent identity: BBa K1033927
display id: BBa_K1033927
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: asPink, pink chromoprotein (incl RBS)
creator: Erik Lundin
created: 2013-09-16T11:00:00Z
modified: 2015-05-08T01:08:50Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1340_
m user id: 0
owner id: 13997
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This chromoprotein from the coral Anemonia sulcata, asCP, naturally exhibits color when expressed.
mutable notes: Synthesized and codon optimized for E coli by GenScript.
mutable provenance: Anemonia sulcata
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_B0034
component: BBa_K1033933
sequence annotation: BBa_K1033933
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K1033927_sequence/1

BBa_B0034
type: Component
persistent identity: component2379049
display id: component2379049
version: 1
top level: asPink
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K1033933
type: Component
persistent identity: component2379051
display id: component2379051
version: 1
top level: asPink
access: public
definition: BBa_K1033933

BBa_K1033933
type: SequenceAnnotation
persistent identity: annotation2379051
display id: annotation2379051
version: 1
top level: asPink
location: https://synbiohub.org/public/igem/BBa_K1033927/annotation2379051/range2379051/1
component: BBa_K1033933

https://synbiohub.org/public/igem/BBa_K1033927/annotation2379051/range2379051/1
type: Range
persistent identity: range2379051
display id: range2379051
version: 1
direction: direction/reverse
top level: asPink
start: 19
end: 720
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2379049
display id: annotation2379049
version: 1
top level: asPink
location: https://synbiohub.org/public/igem/BBa_K1033927/annotation2379049/range2379049/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1033927/annotation2379049/range2379049/1
type: Range
persistent identity: range2379049
display id: range2379049
version: 1
direction: direction/reverse
top level: asPink
start: 1
end: 12
orientation: inline

BBa_K1943000
type: ComponentDefinition
persistent identity: BBa K1943000
display id: BBa_K1943000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: fwYellow, yellow chromoprotein reporter system (Strong Promoter, Strong RBS)
creator: Shixin Lu, Shiqiang Tang
created: 2016-08-28T11:00:00Z
modified: 2016-10-21T08:00:08Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2410_
m user id: 30006
owner id: 29845
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Team Uppsala 2012 chromoprotein attracts many interests because it's more convenient than fluorescent protein for it can be observed with naked eyes. However, characterized data of these proteins are few. Because a single coding region is on the plasmid, we constructed BBa_K1943000 with chromoprotein fwYellow(BBa_K1033910) this year and did characterization. We've constructed a series of plasmids which are all in the same pattern: a strong/weak promoter, a strong/weak RBS and a chromoprotein. We want to monitor the speed of expression of these plasmids in normal incubation conditions (like 37℃ overnight for LB agar plate and 37℃ 180rpm for LB broth). The expression speed and strength of the constructed biobricks will be carefully monitored and gave others a relative scale for using chromoprotein as a reporter gene.
mutable notes: 11
mutable provenance: basic parts
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_K1033910
component: BBa_K880005
component: BBa_B0015
sequence annotation: BBa_K880005
sequence annotation: BBa_B0015
sequence annotation: BBa_K1033910
sequence: https://synbiohub.org/public/igem/BBa_K1943000_sequence/1

BBa_K1033910
type: Component
persistent identity: component2512713
display id: component2512713
version: 1
top level: BBa_K1943000
access: public
definition: fwYellow

BBa_K880005
type: Component
persistent identity: component2512711
display id: component2512711
version: 1
top level: BBa_K1943000
access: public
definition: https://synbiohub.org/public/igem/BBa_K880005/1

BBa_B0015
type: Component
persistent identity: component2512720
display id: component2512720
version: 1
top level: BBa_K1943000
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_K880005
type: SequenceAnnotation
persistent identity: annotation2512711
display id: annotation2512711
version: 1
top level: BBa_K1943000
location: https://synbiohub.org/public/igem/BBa_K1943000/annotation2512711/range2512711/1
component: BBa_K880005

https://synbiohub.org/public/igem/BBa_K1943000/annotation2512711/range2512711/1
type: Range
persistent identity: range2512711
display id: range2512711
version: 1
direction: direction/reverse
top level: BBa_K1943000
start: 1
end: 55
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2512720
display id: annotation2512720
version: 1
top level: BBa_K1943000
location: https://synbiohub.org/public/igem/BBa_K1943000/annotation2512720/range2512720/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K1943000/annotation2512720/range2512720/1
type: Range
persistent identity: range2512720
display id: range2512720
version: 1
direction: direction/reverse
top level: BBa_K1943000
start: 784
end: 912
orientation: inline

BBa_K1033910
type: SequenceAnnotation
persistent identity: annotation2512713
display id: annotation2512713
version: 1
top level: BBa_K1943000
location: https://synbiohub.org/public/igem/BBa_K1943000/annotation2512713/range2512713/1
component: BBa_K1033910

https://synbiohub.org/public/igem/BBa_K1943000/annotation2512713/range2512713/1
type: Range
persistent identity: range2512713
display id: range2512713
version: 1
direction: direction/reverse
top level: BBa_K1943000
start: 62
end: 775
orientation: inline

BBa_K1033930
type: ComponentDefinition
persistent identity: BBa K1033930
display id: BBa_K1033930
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: amilCP, blue/purple chromoprotein (incl RBS)
creator: Erik Lundin
created: 2013-09-16T11:00:00Z
modified: 2015-05-08T01:08:50Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1340_
m user id: 0
owner id: 13997
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This chromoprotein from the coral Acropora millepora, amilCP, naturally exhibits strong color when expressed. The protein has an absorbance maximum at 588 nm giving it a blue/purple color visible to the naked eye, thereby requiring no instruments to observe. The strong color is readily observed in both LB or agar culture, in less than 24 hours of incubation.
mutable notes: Illegal internal restriction site had to be removed (EcoRI).
mutable provenance: Acropora millepora
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_K592009
component: BBa_B0034
sequence annotation: BBa_B0034
sequence annotation: BBa_K592009
sequence: https://synbiohub.org/public/igem/BBa_K1033930_sequence/1

BBa_K592009
type: Component
persistent identity: component2351894
display id: component2351894
version: 1
top level: BBa_K1033930
access: public
definition: https://synbiohub.org/public/igem/BBa_K592009/1

BBa_B0034
type: Component
persistent identity: component2351892
display id: component2351892
version: 1
top level: BBa_K1033930
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2351892
display id: annotation2351892
version: 1
top level: BBa_K1033930
location: https://synbiohub.org/public/igem/BBa_K1033930/annotation2351892/range2351892/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1033930/annotation2351892/range2351892/1
type: Range
persistent identity: range2351892
display id: range2351892
version: 1
direction: direction/reverse
top level: BBa_K1033930
start: 1
end: 12
orientation: inline

BBa_K592009
type: SequenceAnnotation
persistent identity: annotation2351894
display id: annotation2351894
version: 1
top level: BBa_K1033930
location: https://synbiohub.org/public/igem/BBa_K1033930/annotation2351894/range2351894/1
component: BBa_K592009

https://synbiohub.org/public/igem/BBa_K1033930/annotation2351894/range2351894/1
type: Range
persistent identity: range2351894
display id: range2351894
version: 1
direction: direction/reverse
top level: BBa_K1033930
start: 19
end: 687
orientation: inline

BBa_K1033929
type: ComponentDefinition
persistent identity: BBa K1033929
display id: BBa_K1033929
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: aeBlue, blue chromoprotein (incl RBS)
creator: Erik Lundin
created: 2013-09-16T11:00:00Z
modified: 2015-05-08T01:08:50Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1340_
m user id: 0
owner id: 13997
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: aeBlue is a blue chromoprotein extracted from the basal disk of a beadlet anemone Actinia equina. The protein has an absorption maximum at 597nm and a deep blue colour. The protein aeBlue has significant sequence homologies with proteins in the GFP family.
mutable notes: Synthesized and codon optimized for E. coli by Bioneer Corp.
mutable provenance: Actinia equina
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_K864401
component: BBa_B0034
sequence annotation: BBa_K864401
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K1033929_sequence/1

BBa_K864401
type: Component
persistent identity: component2351877
display id: component2351877
version: 1
top level: BBa_K1033929
access: public
definition: https://synbiohub.org/public/igem/BBa_K864401/1

BBa_B0034
type: Component
persistent identity: component2351875
display id: component2351875
version: 1
top level: BBa_K1033929
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K864401
type: SequenceAnnotation
persistent identity: annotation2351877
display id: annotation2351877
version: 1
top level: BBa_K1033929
location: https://synbiohub.org/public/igem/BBa_K1033929/annotation2351877/range2351877/1
component: BBa_K864401

https://synbiohub.org/public/igem/BBa_K1033929/annotation2351877/range2351877/1
type: Range
persistent identity: range2351877
display id: range2351877
version: 1
direction: direction/reverse
top level: BBa_K1033929
start: 19
end: 717
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2351875
display id: annotation2351875
version: 1
top level: BBa_K1033929
location: https://synbiohub.org/public/igem/BBa_K1033929/annotation2351875/range2351875/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1033929/annotation2351875/range2351875/1
type: Range
persistent identity: range2351875
display id: range2351875
version: 1
direction: direction/reverse
top level: BBa_K1033929
start: 1
end: 12
orientation: inline

BBa_K772008
type: ComponentDefinition
persistent identity: BBa K772008
display id: BBa_K772008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: GFP - LacZ protein complex with flag tags
creator: Mustafa Elitok
created: 2012-09-25T11:00:00Z
modified: 2015-05-08T01:13:15Z
discontinued: false
dominant: false
group u list: _1024_
m user id: 0
owner id: 9573
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This part contains a green fluorescent protein (GFP) as a non-reactive chemical compound (anchor) in order to neutralize the active reactive fixed to it, a reporter device (LacZ enzyme catalyses the reaction of the cleavage of X-Gal which results with blue color change) and a TEV protease cleavage site between them to assemble these two proteins. BBa_772001 and BBa_K772002 are assembled to be used as the trigger of detection in the whole system which ends with the intracellular transfection (or activation) of TEV protease. After the activation, it is planned that TEV protease will cleave this anchor-inducer protein complex to release free the reporter. In conclusion, in this system, the inducer would activate a transcription pathway on the targeted inducible gene or the reporter would indicate that there is a change in the whole system. This part contains flag tags extra from BBa_K772006 in the GFP region; because it is planned to be used in the Co-IP/SDS-PAGE assay to be isolated.
mutable notes: Cleavage site, LacZ and GFP sequences are gathered from Registry.
mutable provenance: Note that there is an RBS sequence just before the protein sequence in order to eliminate the need of addition of it.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
sequence annotation: Double Terminator
sequence annotation: GFP
sequence annotation: RBS
sequence annotation: LacZ
sequence annotation: TEV cleavage site
sequence annotation: Anchor-Reporter + Flag
sequence annotation: Flag Tag
sequence: https://synbiohub.org/public/igem/BBa_K772008_sequence/1

Double Terminator
type: SequenceAnnotation
persistent identity: annotation2203915
display id: annotation2203915
version: 1
top level: BBa_K772008
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K772008/annotation2203915/range2203915/1

https://synbiohub.org/public/igem/BBa_K772008/annotation2203915/range2203915/1
type: Range
persistent identity: range2203915
display id: range2203915
version: 1
direction: direction/forward
top level: BBa_K772008
start: 1102
end: 1232
orientation: inline

GFP
type: SequenceAnnotation
persistent identity: annotation2203912
display id: annotation2203912
version: 1
top level: BBa_K772008
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K772008/annotation2203912/range2203912/1

https://synbiohub.org/public/igem/BBa_K772008/annotation2203912/range2203912/1
type: Range
persistent identity: range2203912
display id: range2203912
version: 1
direction: direction/forward
top level: BBa_K772008
start: 47
end: 767
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2203910
display id: annotation2203910
version: 1
top level: BBa_K772008
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K772008/annotation2203910/range2203910/1

https://synbiohub.org/public/igem/BBa_K772008/annotation2203910/range2203910/1
type: Range
persistent identity: range2203910
display id: range2203910
version: 1
direction: direction/forward
top level: BBa_K772008
start: 1
end: 20
orientation: inline

LacZ
type: SequenceAnnotation
persistent identity: annotation2203914
display id: annotation2203914
version: 1
top level: BBa_K772008
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K772008/annotation2203914/range2203914/1

https://synbiohub.org/public/igem/BBa_K772008/annotation2203914/range2203914/1
type: Range
persistent identity: range2203914
display id: range2203914
version: 1
direction: direction/forward
top level: BBa_K772008
start: 858
end: 1101
orientation: inline

TEV cleavage site
type: SequenceAnnotation
persistent identity: annotation2203913
display id: annotation2203913
version: 1
top level: BBa_K772008
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K772008/annotation2203913/range2203913/1

https://synbiohub.org/public/igem/BBa_K772008/annotation2203913/range2203913/1
type: Range
persistent identity: range2203913
display id: range2203913
version: 1
direction: direction/forward
top level: BBa_K772008
start: 768
end: 857
orientation: inline

Anchor-Reporter + Flag
type: SequenceAnnotation
persistent identity: annotation2203916
display id: annotation2203916
version: 1
top level: BBa_K772008
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K772008/annotation2203916/range2203916/1

https://synbiohub.org/public/igem/BBa_K772008/annotation2203916/range2203916/1
type: Range
persistent identity: range2203916
display id: range2203916
version: 1
direction: direction/forward
top level: BBa_K772008
start: 21
end: 1101
orientation: inline

Flag Tag
type: SequenceAnnotation
persistent identity: annotation2203911
display id: annotation2203911
version: 1
top level: BBa_K772008
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K772008/annotation2203911/range2203911/1

https://synbiohub.org/public/igem/BBa_K772008/annotation2203911/range2203911/1
type: Range
persistent identity: range2203911
display id: range2203911
version: 1
direction: direction/forward
top level: BBa_K772008
start: 21
end: 46
orientation: inline

SP-Lipocal
type: ComponentDefinition
persistent identity: BBa K157032
display id: BBa_K157032
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: SP-Lipocalin-GGGSlinker-Tm-betaLactamase(Bla1)
creator: iGEM Team Freiburg 2008
created: 2008-10-25T11:00:00Z
modified: 2015-05-08T01:10:55Z
discontinued: false
dominant: false
experience: experience/None
group u list: _232_
m user id: 0
owner id: 1673
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Part consists of the signal peptide of Erbb1/EGF-receptor, scFV-antiNIP, GGGSflexlinker (extracellular part), the trans-membrane region of the Erbb1/EGF-receptor and the N-terminal part of the split enzyme beta-Lactamase (intracellular part).
mutable notes: none
mutable provenance: Derived by cloning of the parts BBa_K157001, BBa_K157004, BBa_K157013, BBa_K157002 and BBa_I757011. For further information see the description of the parts mentioned above.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: SP-Lipocalin-GGGSlinker-Tm-betaLactamase(Bla1)
sequence annotation: GGGS-Linker
sequence annotation: Scar (Thr, Gly)
sequence annotation: Bla1
sequence annotation: AgeI
sequence annotation: NgoMI
sequence annotation: Lipocalin
sequence annotation: Tm
sequence annotation: SP
sequence: https://synbiohub.org/public/igem/BBa_K157032_sequence/1

SP-Lipocalin-GGGSlinker-Tm-betaLactamase(Bla1)
type: SequenceAnnotation
persistent identity: annotation1990271
display id: annotation1990271
version: 1
top level: SP-Lipocal
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K157032/annotation1990271/range1990271/1

https://synbiohub.org/public/igem/BBa_K157032/annotation1990271/range1990271/1
type: Range
persistent identity: range1990271
display id: range1990271
version: 1
direction: direction/forward
top level: SP-Lipocal
start: 1
end: 1272
orientation: inline

GGGS-Linker
type: SequenceAnnotation
persistent identity: annotation1993336
display id: annotation1993336
version: 1
top level: SP-Lipocal
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157032/annotation1993336/range1993336/1

https://synbiohub.org/public/igem/BBa_K157032/annotation1993336/range1993336/1
type: Range
persistent identity: range1993336
display id: range1993336
version: 1
direction: direction/forward
top level: SP-Lipocal
start: 616
end: 660
orientation: inline

Scar (Thr, Gly)
type: SequenceAnnotation
persistent identity: annotation1993339
display id: annotation1993339
version: 1
top level: SP-Lipocal
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157032/annotation1993339/range1993339/1

https://synbiohub.org/public/igem/BBa_K157032/annotation1993339/range1993339/1
type: Range
persistent identity: range1993339
display id: range1993339
version: 1
direction: direction/forward
top level: SP-Lipocal
start: 736
end: 741
orientation: inline

Scar (Thr, Gly)
type: SequenceAnnotation
persistent identity: annotation1993335
display id: annotation1993335
version: 1
top level: SP-Lipocal
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157032/annotation1993335/range1993335/1

https://synbiohub.org/public/igem/BBa_K157032/annotation1993335/range1993335/1
type: Range
persistent identity: range1993335
display id: range1993335
version: 1
direction: direction/forward
top level: SP-Lipocal
start: 610
end: 615
orientation: inline

Scar (Thr, Gly)
type: SequenceAnnotation
persistent identity: annotation1993337
display id: annotation1993337
version: 1
top level: SP-Lipocal
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157032/annotation1993337/range1993337/1

https://synbiohub.org/public/igem/BBa_K157032/annotation1993337/range1993337/1
type: Range
persistent identity: range1993337
display id: range1993337
version: 1
direction: direction/forward
top level: SP-Lipocal
start: 661
end: 666
orientation: inline

Bla1
type: SequenceAnnotation
persistent identity: annotation1993340
display id: annotation1993340
version: 1
top level: SP-Lipocal
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157032/annotation1993340/range1993340/1

https://synbiohub.org/public/igem/BBa_K157032/annotation1993340/range1993340/1
type: Range
persistent identity: range1993340
display id: range1993340
version: 1
direction: direction/forward
top level: SP-Lipocal
start: 742
end: 1266
orientation: inline

AgeI
type: SequenceAnnotation
persistent identity: annotation1990270
display id: annotation1990270
version: 1
top level: SP-Lipocal
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157032/annotation1990270/range1990270/1

https://synbiohub.org/public/igem/BBa_K157032/annotation1990270/range1990270/1
type: Range
persistent identity: range1990270
display id: range1990270
version: 1
direction: direction/forward
top level: SP-Lipocal
start: 1267
end: 1272
orientation: inline

Scar (Thr, Gly)
type: SequenceAnnotation
persistent identity: annotation1993333
display id: annotation1993333
version: 1
top level: SP-Lipocal
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157032/annotation1993333/range1993333/1

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type: Range
persistent identity: range1993333
display id: range1993333
version: 1
direction: direction/forward
top level: SP-Lipocal
start: 82
end: 87
orientation: inline

NgoMI
type: SequenceAnnotation
persistent identity: annotation1990269
display id: annotation1990269
version: 1
top level: SP-Lipocal
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K157032/annotation1990269/range1990269/1

https://synbiohub.org/public/igem/BBa_K157032/annotation1990269/range1990269/1
type: Range
persistent identity: range1990269
display id: range1990269
version: 1
direction: direction/forward
top level: SP-Lipocal
start: 4
end: 9
orientation: inline

Lipocalin
type: SequenceAnnotation
persistent identity: annotation1993334
display id: annotation1993334
version: 1
top level: SP-Lipocal
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157032/annotation1993334/range1993334/1

https://synbiohub.org/public/igem/BBa_K157032/annotation1993334/range1993334/1
type: Range
persistent identity: range1993334
display id: range1993334
version: 1
direction: direction/forward
top level: SP-Lipocal
start: 88
end: 609
orientation: inline

Tm
type: SequenceAnnotation
persistent identity: annotation1993338
display id: annotation1993338
version: 1
top level: SP-Lipocal
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157032/annotation1993338/range1993338/1

https://synbiohub.org/public/igem/BBa_K157032/annotation1993338/range1993338/1
type: Range
persistent identity: range1993338
display id: range1993338
version: 1
direction: direction/forward
top level: SP-Lipocal
start: 667
end: 735
orientation: inline

SP
type: SequenceAnnotation
persistent identity: annotation1993332
display id: annotation1993332
version: 1
top level: SP-Lipocal
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K157032/annotation1993332/range1993332/1

https://synbiohub.org/public/igem/BBa_K157032/annotation1993332/range1993332/1
type: Range
persistent identity: range1993332
display id: range1993332
version: 1
direction: direction/forward
top level: SP-Lipocal
start: 10
end: 81
orientation: inline

gfasPurple
type: ComponentDefinition
persistent identity: BBa K1033918
display id: BBa_K1033918
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: gfasPurple, purple chromoprotein (incl RBS)
creator: Erik Gullberg
created: 2013-09-16T11:00:00Z
modified: 2015-05-08T01:08:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1340_
m user id: 0
owner id: 13997
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This chromoprotein from the coral Galaxea fascicularis, gfasPurple, naturally exhibits strong color when expressed.
mutable notes: Synthesized and codon optimized for E coli by GenScript.
mutable provenance: Galaxea fascicularis
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_K1033919
component: BBa_B0034
sequence annotation: BBa_B0034
sequence annotation: BBa_K1033919
sequence: https://synbiohub.org/public/igem/BBa_K1033918_sequence/1

BBa_K1033919
type: Component
persistent identity: component2351816
display id: component2351816
version: 1
top level: gfasPurple
access: public
definition: https://synbiohub.org/public/igem/BBa_K1033919/1

BBa_B0034
type: Component
persistent identity: component2351814
display id: component2351814
version: 1
top level: gfasPurple
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2351814
display id: annotation2351814
version: 1
top level: gfasPurple
location: https://synbiohub.org/public/igem/BBa_K1033918/annotation2351814/range2351814/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1033918/annotation2351814/range2351814/1
type: Range
persistent identity: range2351814
display id: range2351814
version: 1
direction: direction/reverse
top level: gfasPurple
start: 1
end: 12
orientation: inline

BBa_K1033919
type: SequenceAnnotation
persistent identity: annotation2351816
display id: annotation2351816
version: 1
top level: gfasPurple
location: https://synbiohub.org/public/igem/BBa_K1033918/annotation2351816/range2351816/1
component: BBa_K1033919

https://synbiohub.org/public/igem/BBa_K1033918/annotation2351816/range2351816/1
type: Range
persistent identity: range2351816
display id: range2351816
version: 1
direction: direction/reverse
top level: gfasPurple
start: 19
end: 687
orientation: inline

BY-Toggle
type: ComponentDefinition
persistent identity: BBa K1908000
display id: BBa_K1908000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Blue-Yellow fluorescent protein toggle switch using pLac and OmpC promoter.
creator: Ryan Kawalerski, Sarah Heacox, Jonathan Krog
created: 2016-10-13T11:00:00Z
modified: 2016-10-18T11:04:57Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2374_
m user id: 29538
owner id: 29544
owning group id: 9
status: status/Planning
bookmark: true
mutable description: .
mutable notes: .
mutable provenance: .
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K592009
component: BBa_R0082
component: BBa_K1033910
component: BBa_C0012
component: BBa_R0011
component: BBa_J61100
component: BBa_B0015
sequence annotation: BBa_J61100
sequence annotation: BBa_R0082
sequence annotation: BBa_C0012
sequence annotation: BBa_B0015
sequence annotation: BBa_K592009
sequence annotation: BBa_K1033910
sequence annotation: BBa_R0011
sequence: https://synbiohub.org/public/igem/BBa_K1908000_sequence/1

BBa_K592009
type: Component
persistent identity: component2520436
display id: component2520436
version: 1
top level: BY-Toggle
access: public
definition: https://synbiohub.org/public/igem/BBa_K592009/1

BBa_R0082
type: Component
persistent identity: component2520433
display id: component2520433
version: 1
top level: BY-Toggle
access: public
definition: https://synbiohub.org/public/igem/BBa_R0082/1

BBa_K1033910
type: Component
persistent identity: component2520420
display id: component2520420
version: 1
top level: BY-Toggle
access: public
definition: fwYellow

BBa_C0012
type: Component
persistent identity: component2520438
display id: component2520438
version: 1
top level: BY-Toggle
access: public
definition: https://synbiohub.org/public/igem/BBa_C0012/1

BBa_R0011
type: Component
persistent identity: component2520413
display id: component2520413
version: 1
top level: BY-Toggle
access: public
definition: https://synbiohub.org/public/igem/BBa_R0011/1

BBa_J61100
type: Component
persistent identity: component2520418
display id: component2520418
version: 1
top level: BY-Toggle
access: public
definition: https://synbiohub.org/public/igem/BBa_J61100/1

BBa_B0015
type: Component
persistent identity: component2520447
display id: component2520447
version: 1
top level: BY-Toggle
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_B0015
type: Component
persistent identity: component2520427
display id: component2520427
version: 1
top level: BY-Toggle
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_J61100
type: Component
persistent identity: component2520434
display id: component2520434
version: 1
top level: BY-Toggle
access: public
definition: https://synbiohub.org/public/igem/BBa_J61100/1

BBa_J61100
type: SequenceAnnotation
persistent identity: annotation2520434
display id: annotation2520434
version: 1
top level: BY-Toggle
location: https://synbiohub.org/public/igem/BBa_K1908000/annotation2520434/range2520434/1
component: BBa_J61100

https://synbiohub.org/public/igem/BBa_K1908000/annotation2520434/range2520434/1
type: Range
persistent identity: range2520434
display id: range2520434
version: 1
direction: direction/reverse
top level: BY-Toggle
start: 1057
end: 1068
orientation: inline

BBa_R0082
type: SequenceAnnotation
persistent identity: annotation2520433
display id: annotation2520433
version: 1
top level: BY-Toggle
location: https://synbiohub.org/public/igem/BBa_K1908000/annotation2520433/range2520433/1
component: BBa_R0082

https://synbiohub.org/public/igem/BBa_K1908000/annotation2520433/range2520433/1
type: Range
persistent identity: range2520433
display id: range2520433
version: 1
direction: direction/reverse
top level: BY-Toggle
start: 941
end: 1048
orientation: inline

BBa_J61100
type: SequenceAnnotation
persistent identity: annotation2520418
display id: annotation2520418
version: 1
top level: BY-Toggle
location: https://synbiohub.org/public/igem/BBa_K1908000/annotation2520418/range2520418/1
component: BBa_J61100

https://synbiohub.org/public/igem/BBa_K1908000/annotation2520418/range2520418/1
type: Range
persistent identity: range2520418
display id: range2520418
version: 1
direction: direction/reverse
top level: BY-Toggle
start: 64
end: 75
orientation: inline

BBa_C0012
type: SequenceAnnotation
persistent identity: annotation2520438
display id: annotation2520438
version: 1
top level: BY-Toggle
location: https://synbiohub.org/public/igem/BBa_K1908000/annotation2520438/range2520438/1
component: BBa_C0012

https://synbiohub.org/public/igem/BBa_K1908000/annotation2520438/range2520438/1
type: Range
persistent identity: range2520438
display id: range2520438
version: 1
direction: direction/forward
top level: BY-Toggle
start: 1750
end: 2877
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2520427
display id: annotation2520427
version: 1
top level: BY-Toggle
location: https://synbiohub.org/public/igem/BBa_K1908000/annotation2520427/range2520427/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K1908000/annotation2520427/range2520427/1
type: Range
persistent identity: range2520427
display id: range2520427
version: 1
direction: direction/reverse
top level: BY-Toggle
start: 804
end: 932
orientation: inline

BBa_K592009
type: SequenceAnnotation
persistent identity: annotation2520436
display id: annotation2520436
version: 1
top level: BY-Toggle
location: https://synbiohub.org/public/igem/BBa_K1908000/annotation2520436/range2520436/1
component: BBa_K592009

https://synbiohub.org/public/igem/BBa_K1908000/annotation2520436/range2520436/1
type: Range
persistent identity: range2520436
display id: range2520436
version: 1
direction: direction/reverse
top level: BY-Toggle
start: 1075
end: 1743
orientation: inline

BBa_K1033910
type: SequenceAnnotation
persistent identity: annotation2520420
display id: annotation2520420
version: 1
top level: BY-Toggle
location: https://synbiohub.org/public/igem/BBa_K1908000/annotation2520420/range2520420/1
component: BBa_K1033910

https://synbiohub.org/public/igem/BBa_K1908000/annotation2520420/range2520420/1
type: Range
persistent identity: range2520420
display id: range2520420
version: 1
direction: direction/reverse
top level: BY-Toggle
start: 82
end: 795
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2520447
display id: annotation2520447
version: 1
top level: BY-Toggle
location: https://synbiohub.org/public/igem/BBa_K1908000/annotation2520447/range2520447/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K1908000/annotation2520447/range2520447/1
type: Range
persistent identity: range2520447
display id: range2520447
version: 1
direction: direction/reverse
top level: BY-Toggle
start: 2911
end: 3039
orientation: inline

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation2520413
display id: annotation2520413
version: 1
top level: BY-Toggle
location: https://synbiohub.org/public/igem/BBa_K1908000/annotation2520413/range2520413/1
component: BBa_R0011

https://synbiohub.org/public/igem/BBa_K1908000/annotation2520413/range2520413/1
type: Range
persistent identity: range2520413
display id: range2520413
version: 1
direction: direction/forward
top level: BY-Toggle
start: 1
end: 54
orientation: inline

BBa_K1033915
type: ComponentDefinition
persistent identity: BBa K1033915
display id: BBa_K1033915
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: amajLime, yellow-green chromoprotein (incl RBS)
creator: Erik Gullberg
created: 2013-09-16T11:00:00Z
modified: 2015-05-08T01:08:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1340_
m user id: 0
owner id: 13997
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This chromoprotein from the coral Anemonia majano, amajLime, naturally exhibits strong color when expressed.
mutable notes: Synthesized and codon optimized for E coli by GenScript.
mutable provenance: Anemonia majano
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_K1033916
component: BBa_B0034
sequence annotation: BBa_B0034
sequence annotation: BBa_K1033916
sequence: https://synbiohub.org/public/igem/BBa_K1033915_sequence/1

BBa_K1033916
type: Component
persistent identity: component2350102
display id: component2350102
version: 1
top level: BBa_K1033915
access: public
definition: https://synbiohub.org/public/igem/BBa_K1033916/1

BBa_B0034
type: Component
persistent identity: component2350100
display id: component2350100
version: 1
top level: BBa_K1033915
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2350100
display id: annotation2350100
version: 1
top level: BBa_K1033915
location: https://synbiohub.org/public/igem/BBa_K1033915/annotation2350100/range2350100/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1033915/annotation2350100/range2350100/1
type: Range
persistent identity: range2350100
display id: range2350100
version: 1
direction: direction/reverse
top level: BBa_K1033915
start: 1
end: 12
orientation: inline

BBa_K1033916
type: SequenceAnnotation
persistent identity: annotation2350102
display id: annotation2350102
version: 1
top level: BBa_K1033915
location: https://synbiohub.org/public/igem/BBa_K1033915/annotation2350102/range2350102/1
component: BBa_K1033916

https://synbiohub.org/public/igem/BBa_K1033915/annotation2350102/range2350102/1
type: Range
persistent identity: range2350102
display id: range2350102
version: 1
direction: direction/reverse
top level: BBa_K1033915
start: 19
end: 711
orientation: inline

BBa_K1188000
type: ComponentDefinition
persistent identity: BBa K1188000
display id: BBa_K1188000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: AmilCP screening part
creator: Bill Heymann, Phil Jensen
created: 2013-08-13T11:00:00Z
modified: 2015-05-08T01:09:37Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1501_
m user id: 0
owner id: 17729
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This part has a strong constitutive promoter (J23100) the standard RBS (B0034) and AmilCP (K592009). When assembled into a plasmid it gives a strong blue color. The reason we created this part is the default backones all have essentially the same construct but using RFP instead. With an insert with a different color it made it much easier to debug problems with digests, ligations and transformations. We also used it to try to find a problem with some strange background issues we had. This was part also use to create a lot of AmilCP that was used in our protein purification experiments.
mutable notes: We needed a strong blue color to make it easy to distinguish this from a red carry through. We used a strong anderson promotor (J23100), standard RBS and hooked it to AmilCP.
mutable provenance: J23100, K592009 and B0034 came from the registry.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0034
component: BBa_J23100
component: BBa_K592009
sequence annotation: BBa_K592009
sequence annotation: BBa_B0034
sequence annotation: BBa_J23100
sequence: https://synbiohub.org/public/igem/BBa_K1188000_sequence/1

BBa_B0034
type: Component
persistent identity: component2331811
display id: component2331811
version: 1
top level: BBa_K1188000
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_J23100
type: Component
persistent identity: component2331809
display id: component2331809
version: 1
top level: BBa_K1188000
access: public
definition: https://synbiohub.org/public/igem/BBa_J23100/1

BBa_K592009
type: Component
persistent identity: component2331813
display id: component2331813
version: 1
top level: BBa_K1188000
access: public
definition: https://synbiohub.org/public/igem/BBa_K592009/1

BBa_K592009
type: SequenceAnnotation
persistent identity: annotation2331813
display id: annotation2331813
version: 1
top level: BBa_K1188000
location: https://synbiohub.org/public/igem/BBa_K1188000/annotation2331813/range2331813/1
component: BBa_K592009

https://synbiohub.org/public/igem/BBa_K1188000/annotation2331813/range2331813/1
type: Range
persistent identity: range2331813
display id: range2331813
version: 1
direction: direction/reverse
top level: BBa_K1188000
start: 62
end: 730
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2331811
display id: annotation2331811
version: 1
top level: BBa_K1188000
location: https://synbiohub.org/public/igem/BBa_K1188000/annotation2331811/range2331811/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1188000/annotation2331811/range2331811/1
type: Range
persistent identity: range2331811
display id: range2331811
version: 1
direction: direction/reverse
top level: BBa_K1188000
start: 44
end: 55
orientation: inline

BBa_J23100
type: SequenceAnnotation
persistent identity: annotation2331809
display id: annotation2331809
version: 1
top level: BBa_K1188000
location: https://synbiohub.org/public/igem/BBa_K1188000/annotation2331809/range2331809/1
component: BBa_J23100

https://synbiohub.org/public/igem/BBa_K1188000/annotation2331809/range2331809/1
type: Range
persistent identity: range2331809
display id: range2331809
version: 1
direction: direction/reverse
top level: BBa_K1188000
start: 1
end: 35
orientation: inline

BBa_K147002
type: ComponentDefinition
persistent identity: BBa K147002
display id: BBa_K147002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: xylE
creator: Nathan Puhl
created: 2008-10-27T12:00:00Z
modified: 2015-05-08T01:10:39Z
discontinued: false
dominant: true
group u list: _129_242_
m user id: 0
owner id: 1757
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Blah Blah Blah
mutable notes: Blah Blah Blah
mutable provenance: Blah Blah Blah
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K147002_sequence/1

BBa_K2056003
type: ComponentDefinition
persistent identity: BBa K2056003
display id: BBa_K2056003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Carbon Monoxide sensor device (pCooF-Blue Chromoprotein)
creator: Muhammad Ismail
created: 2016-10-12T11:00:00Z
modified: 2016-10-13T12:36:12Z
discontinued: false
dominant: false
group u list: _2524_
m user id: 33019
owner id: 33019
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This part is the carbon monoxide (CO) sensing module of the 'Inspector NOxCO' device. 'Inspector NOxCO' is a bacterial sensor for carbon monoxide (CO) and oxides of nitrogen (NOx) in vehicle emissions. This part consists of the Anderson constitutive promoter(BBa_J23110) and RBS(B0030) with the coding sequence for CooA(BBa_K352001) a CO dependent transcriptional activator and single terminator(B0010) followed by CooA activated strong promoter pCooF(K352002) with RBS(B0030) and 'amilCP, blue chromoprotein' with terminator(BBa_B0012). If CO is present, the constitutive expressed CooA will bind to it and then bind to the pCOoF promoter activating transcription of the blue chromoprotein.
mutable notes: The selection of the RBS and Constitutive promoter. Because of limitations of repeats that could be synthesized, we had to give up on the double terminators in the original design and go with single different terminators. A 70bp spacer(B0040) has been added for transcriptional efficiency.
mutable provenance: All parts in this sequence come from the Registry and have been listed. However, the complete part has been synthesized from IDT using their free gBLocks offer.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
component: BBa_B0010
component: BBa_K592009
component: BBa_B0030
component: BBa_J23110
component: BBa_B0040
component: BBa_K352001
component: BBa_K352002
component: BBa_B0012
sequence annotation: BBa_B0030
sequence annotation: BBa_J23110
sequence annotation: BBa_K592009
sequence annotation: BBa_B0010
sequence annotation: BBa_K352001
sequence annotation: BBa_B0040
sequence annotation: BBa_K352002
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_K2056003_sequence/1

BBa_B0010
type: Component
persistent identity: component2499197
display id: component2499197
version: 1
top level: BBa_K2056003
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_K592009
type: Component
persistent identity: component2499213
display id: component2499213
version: 1
top level: BBa_K2056003
access: public
definition: https://synbiohub.org/public/igem/BBa_K592009/1

BBa_B0030
type: Component
persistent identity: component2499210
display id: component2499210
version: 1
top level: BBa_K2056003
access: public
definition: https://synbiohub.org/public/igem/BBa_B0030/1

BBa_J23110
type: Component
persistent identity: component2499191
display id: component2499191
version: 1
top level: BBa_K2056003
access: public
definition: https://synbiohub.org/public/igem/BBa_J23110/1

BBa_B0040
type: Component
persistent identity: component2499200
display id: component2499200
version: 1
top level: BBa_K2056003
access: public
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BBa_K352001
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BBa_K352002
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BBa_B0030
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BBa_B0012
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BBa_B0030
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BBa_B0030
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BBa_J23110
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BBa_K592009
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BBa_B0010
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BBa_K352001
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BBa_B0040
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BBa_K352002
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BBa_B0012
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BBa_K1033908
type: ComponentDefinition
persistent identity: BBa K1033908
display id: BBa_K1033908
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: fwYellow, yellow chromoprotein (incl RBS, J23106)
creator: Sabri Jamal
created: 2013-09-16T11:00:00Z
modified: 2015-05-08T01:08:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1340_
m user id: 0
owner id: 13997
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This chromoprotein naturally exhibits strong color when expressed.
mutable notes: Cloned from FezziwigYFP vector, DNA 2.0.
mutable provenance: Synthetic chromoprotein from DNA 2.0.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_J23106
component: BBa_K1033909
sequence annotation: BBa_J23106
sequence annotation: BBa_K1033909
sequence: https://synbiohub.org/public/igem/BBa_K1033908_sequence/1

BBa_J23106
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BBa_K1033909
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definition: fwYellow

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