//function/recombination/p22
type: Collection
persistent identity: function recombination p22
display id: function_recombination_p22
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
owned by: james
owned by: myers
member: BBa_I11031
member: https://synbiohub.org/public/igem/BBa_I11032/1
member: https://synbiohub.org/public/igem/BBa_I11033/1
member: BBa_I11030

//function/recombination
type: Collection
persistent identity: function recombination
display id: function_recombination
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
owned by: james
owned by: myers
member: Hin
member: BBa_K112245
member: Bxb1_Int
member: BBa_K112215
member: BBa_J70007
member: ihfB
member: //function/recombination/xer
member: //function/recombination/fim
member: BBa_I716213
member: BBa_I11031
member: BBa_K863201
member: BBa_K112208
member: BBa_K361011
member: BBa_K112100
member: BBa_K1015999
member: BBa_K108023
member: BBa_K112212
member: BBa_K112122
member: //function/recombination/hin
member: //function/recombination/flp
member: BBa_K112209
member: BBa_K108022
member: BBa_K112214
member: BBa_K1926021
member: //function/recombination/cre
member: BBa_J61030
member: Rci
member: BBa_K112207
member: BBa_K112211
member: Bxb1_Xis
member: BBa_K1926012
member: //function/recombination/lambda
member: BBa_K108024
member: Hin LVA
member: BBa_K112220
member: BBa_K112216
member: BBa_I11030
member: BBa_I716212
member: BBa_K112221
member: BBa_K112101
member: ihfA
member: BBa_I11021
member: BBa_K112217
member: BBa_S04524
member: BBa_K112115
member: BBa_K112222
member: BBa_I11020
member: BBa_K112219
member: BBa_K112223
member: //function/recombination/p22
member: Cre
member: BBa_K112210

//function/recombination/cre
type: Collection
persistent identity: function recombination cre
display id: function_recombination_cre
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
owned by: james
owned by: myers
member: https://synbiohub.org/public/igem/BBa_K1926011/1
member: https://synbiohub.org/public/igem/BBa_I718017/1
member: https://synbiohub.org/public/igem/BBa_K1680017/1
member: https://synbiohub.org/public/igem/BBa_I718008/1
member: https://synbiohub.org/public/igem/BBa_K1680020/1
member: https://synbiohub.org/public/igem/BBa_K886001/1
member: BBa_I716213
member: https://synbiohub.org/public/igem/BBa_K416002/1
member: https://synbiohub.org/public/igem/BBa_K886003/1
member: https://synbiohub.org/public/igem/BBa_K1680022/1
member: https://synbiohub.org/public/igem/BBa_K1680005/1
member: https://synbiohub.org/public/igem/BBa_I718016/1
member: https://synbiohub.org/public/igem/BBa_K1680018/1
member: https://synbiohub.org/public/igem/BBa_K1680019/1
member: https://synbiohub.org/public/igem/BBa_I716208/1
member: https://synbiohub.org/public/igem/BBa_I716436/1
member: BBa_K112100
member: https://synbiohub.org/public/igem/BBa_K886000/1
member: BBa_I716212
member: https://synbiohub.org/public/igem/BBa_K132027/1
member: https://synbiohub.org/public/igem/BBa_I718007/1
member: BBa_K112122
member: https://synbiohub.org/public/igem/BBa_K1680023/1
member: BBa_K112101
member: https://synbiohub.org/public/igem/BBa_K1680007/1
member: https://synbiohub.org/public/igem/BBa_K132028/1
member: https://synbiohub.org/public/igem/BBa_K1680024/1
member: https://synbiohub.org/public/igem/BBa_J61046/1
member: BBa_K112115
member: https://synbiohub.org/public/igem/BBa_K112102/1
member: https://synbiohub.org/public/igem/BBa_K082011/1
member: https://synbiohub.org/public/igem/BBa_K1680008/1
member: https://synbiohub.org/public/igem/BBa_K1680021/1
member: https://synbiohub.org/public/igem/BBa_I716438/1
member: https://synbiohub.org/public/igem/BBa_K886002/1
member: Cre
member: https://synbiohub.org/public/igem/BBa_K1680025/1

//function/recombination/hin
type: Collection
persistent identity: function recombination hin
display id: function_recombination_hin
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
owned by: james
owned by: myers
member: https://synbiohub.org/public/igem/BBa_J44013/1
member: https://synbiohub.org/public/igem/BBa_I715042/1
member: https://synbiohub.org/public/igem/BBa_I715049/1
member: Hin
member: https://synbiohub.org/public/igem/BBa_J3104/1
member: https://synbiohub.org/public/igem/BBa_I715047/1
member: https://synbiohub.org/public/igem/BBa_J3106/1
member: https://synbiohub.org/public/igem/BBa_I715044/1
member: https://synbiohub.org/public/igem/BBa_J44009/1
member: https://synbiohub.org/public/igem/BBa_J44003/1
member: https://synbiohub.org/public/igem/BBa_J44010/1
member: https://synbiohub.org/public/igem/BBa_I715051/1
member: https://synbiohub.org/public/igem/BBa_J44012/1
member: https://synbiohub.org/public/igem/BBa_I715043/1
member: Hin LVA
member: https://synbiohub.org/public/igem/BBa_J3105/1
member: https://synbiohub.org/public/igem/BBa_S03639/1
member: https://synbiohub.org/public/igem/BBa_J3107/1
member: https://synbiohub.org/public/igem/BBa_J3108/1
member: https://synbiohub.org/public/igem/BBa_J3103/1
member: https://synbiohub.org/public/igem/BBa_J44011/1
member: https://synbiohub.org/public/igem/BBa_I715037/1
member: https://synbiohub.org/public/igem/BBa_J3101/1
member: https://synbiohub.org/public/igem/BBa_J44000/1

//function/recombination/fim
type: Collection
persistent identity: function recombination fim
display id: function_recombination_fim
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
owned by: james
owned by: myers
member: https://synbiohub.org/public/igem/BBa_K137060/1
member: https://synbiohub.org/public/igem/BBa_K1632007/1
member: https://synbiohub.org/public/igem/BBa_K137008/1
member: https://synbiohub.org/public/igem/BBa_K137058/1
member: https://synbiohub.org/public/igem/BBa_K137007/1
member: https://synbiohub.org/public/igem/BBa_K137059/1
member: https://synbiohub.org/public/igem/BBa_K137057/1
member: https://synbiohub.org/public/igem/BBa_K1632000/1
member: https://synbiohub.org/public/igem/BBa_K137010/1
member: https://synbiohub.org/public/igem/BBa_K137062/1
member: https://synbiohub.org/public/igem/BBa_K137061/1

//function/recombination/lambda
type: Collection
persistent identity: function recombination lambda
display id: function_recombination_lambda
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
owned by: james
owned by: myers
member: https://synbiohub.org/public/igem/BBa_K112133/1
member: https://synbiohub.org/public/igem/BBa_K112200/1
member: https://synbiohub.org/public/igem/BBa_K112247/1
member: https://synbiohub.org/public/igem/BBa_K112202/1
member: https://synbiohub.org/public/igem/BBa_K112205/1
member: https://synbiohub.org/public/igem/BBa_K112234/1
member: BBa_I11021
member: https://synbiohub.org/public/igem/BBa_K112204/1
member: https://synbiohub.org/public/igem/BBa_K112237/1
member: https://synbiohub.org/public/igem/BBa_K112327/1
member: https://synbiohub.org/public/igem/BBa_K617000/1
member: https://synbiohub.org/public/igem/BBa_K112142/1
member: https://synbiohub.org/public/igem/BBa_K112246/1
member: https://synbiohub.org/public/igem/BBa_K112203/1
member: https://synbiohub.org/public/igem/BBa_I11023/1
member: BBa_I11020
member: https://synbiohub.org/public/igem/BBa_K112248/1
member: https://synbiohub.org/public/igem/BBa_I11022/1
member: https://synbiohub.org/public/igem/BBa_K112137/1
member: https://synbiohub.org/public/igem/BBa_K112132/1
member: https://synbiohub.org/public/igem/BBa_K112001/1
member: https://synbiohub.org/public/igem/BBa_K112141/1

//function/recombination/flp
type: Collection
persistent identity: function recombination flp
display id: function_recombination_flp
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
owned by: james
owned by: myers
member: https://synbiohub.org/public/igem/BBa_J61027/1
member: https://synbiohub.org/public/igem/BBa_J61024/1
member: https://synbiohub.org/public/igem/BBa_K551000/1
member: https://synbiohub.org/public/igem/BBa_J61020/1
member: https://synbiohub.org/public/igem/BBa_J61025/1
member: https://synbiohub.org/public/igem/BBa_J61026/1
member: https://synbiohub.org/public/igem/BBa_J72001/1
member: https://synbiohub.org/public/igem/BBa_K112138/1
member: https://synbiohub.org/public/igem/BBa_K313002/1
member: https://synbiohub.org/public/igem/BBa_K551001/1
member: https://synbiohub.org/public/igem/BBa_K1015001/1

//function/recombination/xer
type: Collection
persistent identity: function recombination xer
display id: function_recombination_xer
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
owned by: james
owned by: myers
member: https://synbiohub.org/public/igem/BBa_I742101/1
member: https://synbiohub.org/public/igem/BBa_I742102/1

BBa_K361011
type: ComponentDefinition
persistent identity: BBa K361011
display id: BBa_K361011
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Rci recombinase system
creator: Jacky, Fong Chuen, Loo
created: 2010-10-23T11:00:00Z
modified: 2015-05-08T01:12:12Z
discontinued: false
dominant: false
experience: experience/None
group u list: _476_
m user id: 0
owner id: 6123
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This is the protein genertors of Rci site-specific recombinase BBa_K361000, which is being linked with a promotor and a ribosome binding site in front of it, a double terminator after it.
mutable notes: This is the optimal system for Rci site-specific recombinase production.
mutable provenance: The promotor is come from lacI regulated one: BBa_R0010 The ribosome binding site is come from the defines RBS efficiency equal to one : BBa_B0034 The terminator is come from a doble terminator with bidirectional termination: BBa_B0014
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_S04509
component: BBa_S04524
sequence annotation: BBa_S04509
sequence annotation: BBa_S04524
sequence: https://synbiohub.org/public/igem/BBa_K361011_sequence/1

BBa_S04509
type: Component
persistent identity: component2095262
display id: component2095262
version: 1
top level: BBa_K361011
access: public
definition: https://synbiohub.org/public/igem/BBa_S04509/1

BBa_S04524
type: Component
persistent identity: component2095272
display id: component2095272
version: 1
top level: BBa_K361011
access: public
definition: BBa_S04524

BBa_S04509
type: SequenceAnnotation
persistent identity: annotation2095262
display id: annotation2095262
version: 1
top level: BBa_K361011
location: https://synbiohub.org/public/igem/BBa_K361011/annotation2095262/range2095262/1
component: BBa_S04509

https://synbiohub.org/public/igem/BBa_K361011/annotation2095262/range2095262/1
type: Range
persistent identity: range2095262
display id: range2095262
version: 1
direction: direction/reverse
top level: BBa_K361011
start: 1
end: 220
orientation: inline

BBa_S04524
type: SequenceAnnotation
persistent identity: annotation2095272
display id: annotation2095272
version: 1
top level: BBa_K361011
location: https://synbiohub.org/public/igem/BBa_K361011/annotation2095272/range2095272/1
component: BBa_S04524

https://synbiohub.org/public/igem/BBa_K361011/annotation2095272/range2095272/1
type: Range
persistent identity: range2095272
display id: range2095272
version: 1
direction: direction/reverse
top level: BBa_K361011
start: 227
end: 1484
orientation: inline

Cre
type: ComponentDefinition
persistent identity: BBa J61047
display id: BBa_J61047
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Cre DNA recombinase
creator: John Anderson
created: 2007-02-20T12:00:00Z
modified: 2015-08-31T02:03:00Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable description: bob
mutable notes: bob
mutable provenance: bob
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_J61047_sequence/1

Hin LVA
type: ComponentDefinition
persistent identity: BBa J31001
display id: BBa_J31001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: DNA invertase Hin tagged with LVA
creator: Erin Zwack, Sabriya Rosemond
created: 2006-07-11T11:00:00Z
modified: 2015-08-31T04:08:45Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _61_
m user id: 0
owner id: 918
owning group id: 61
sample status: In stock
status: status/Available
bookmark: true
mutable description: This part produces the Hin protein with an LVA degredation tag. We are currently looking into this as a way to ensure no Hin is floating around once we turn off the Hin promoter.
mutable notes: None really
mutable provenance: Salmonella typhimurium and the the Hin part without LVA.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: LVA
sequence annotation: Stop codon
sequence annotation: Hin
sequence annotation: Fwd primer J31001
sequence annotation: Rev primer J31001
sequence: https://synbiohub.org/public/igem/BBa_J31001_sequence/1

LVA
type: SequenceAnnotation
persistent identity: annotation1910566
display id: annotation1910566
version: 1
top level: Hin LVA
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_J31001/annotation1910566/range1910566/1

https://synbiohub.org/public/igem/BBa_J31001/annotation1910566/range1910566/1
type: Range
persistent identity: range1910566
display id: range1910566
version: 1
direction: direction/forward
top level: Hin LVA
start: 571
end: 609
orientation: inline

Stop codon
type: SequenceAnnotation
persistent identity: annotation1910567
display id: annotation1910567
version: 1
top level: Hin LVA
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_J31001/annotation1910567/range1910567/1

https://synbiohub.org/public/igem/BBa_J31001/annotation1910567/range1910567/1
type: Range
persistent identity: range1910567
display id: range1910567
version: 1
direction: direction/forward
top level: Hin LVA
start: 610
end: 612
orientation: inline

Hin
type: SequenceAnnotation
persistent identity: annotation1910564
display id: annotation1910564
version: 1
top level: Hin LVA
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J31001/annotation1910564/range1910564/1

https://synbiohub.org/public/igem/BBa_J31001/annotation1910564/range1910564/1
type: Range
persistent identity: range1910564
display id: range1910564
version: 1
direction: direction/forward
top level: Hin LVA
start: 1
end: 570
orientation: inline

Fwd primer J31001
type: SequenceAnnotation
persistent identity: annotation1910565
display id: annotation1910565
version: 1
top level: Hin LVA
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_J31001/annotation1910565/range1910565/1

https://synbiohub.org/public/igem/BBa_J31001/annotation1910565/range1910565/1
type: Range
persistent identity: range1910565
display id: range1910565
version: 1
direction: direction/forward
top level: Hin LVA
start: 1
end: 3
orientation: inline

Rev primer J31001
type: SequenceAnnotation
persistent identity: annotation1910570
display id: annotation1910570
version: 1
top level: Hin LVA
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_J31001/annotation1910570/range1910570/1

https://synbiohub.org/public/igem/BBa_J31001/annotation1910570/range1910570/1
type: Range
persistent identity: range1910570
display id: range1910570
version: 1
direction: direction/reverse
top level: Hin LVA
start: 551
end: 612
orientation: inline

BBa_K112115
type: ComponentDefinition
persistent identity: BBa K112115
display id: BBa_K112115
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: a~Cre
creator: Bing Xia
created: 2008-10-20T11:00:00Z
modified: 2015-05-08T01:09:14Z
discontinued: false
dominant: false
experience: experience/None
group u list: _224_
m user id: 0
owner id: 2998
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Cre recombinase gene, with a spacer for the rbs prepended. ---- This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at: [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
mutable provenance: PCR mv025/mv002 on pBca9145-Bca1010 (1067bp, EcoRI/BamHI/DpnI) Sub into pBca1256 (EcoRI/BamHI, 2472+910, L) Product is pBca1256-K112115 ----------------------------------------------- mv025 Forward Biobricking of a~Cre! ccataGAATTCatgAGATCTttaaatgtccaatttactgaccgtacacc mv002 Reverse Biobricking of <Cre! cgttaGGATCCttaatcgccatcttccagc
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K112115_sequence/1

BBa_I11031
type: ComponentDefinition
persistent identity: BBa I11031
display id: BBa_I11031
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: excisionase from E. coli phage P22 (removes prophage from host genome)
creator: mschomp
created: 2004-09-02T11:00:00Z
modified: 2015-08-31T04:07:30Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2_
m user id: 0
owner id: 102
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: P22 Excisionase with AAV tag
mutable provenance: Nuc Acid Res, 2001; 29(11):2205-2216
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: stop
sequence annotation: Help:Barcodes
sequence annotation: Xis p22
sequence annotation: AAV
sequence: https://synbiohub.org/public/igem/BBa_I11031_sequence/1

stop
type: SequenceAnnotation
persistent identity: annotation1198221
display id: annotation1198221
version: 1
top level: BBa_I11031
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_I11031/annotation1198221/range1198221/1

https://synbiohub.org/public/igem/BBa_I11031/annotation1198221/range1198221/1
type: Range
persistent identity: range1198221
display id: range1198221
version: 1
direction: direction/forward
top level: BBa_I11031
start: 382
end: 387
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2214030
display id: annotation2214030
version: 1
top level: BBa_I11031
role: SO:0000807
role: feature/barcode
location: https://synbiohub.org/public/igem/BBa_I11031/annotation2214030/range2214030/1

https://synbiohub.org/public/igem/BBa_I11031/annotation2214030/range2214030/1
type: Range
persistent identity: range2214030
display id: range2214030
version: 1
direction: direction/forward
top level: BBa_I11031
start: 388
end: 412
orientation: inline

Xis p22
type: SequenceAnnotation
persistent identity: annotation1198186
display id: annotation1198186
version: 1
top level: BBa_I11031
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_I11031/annotation1198186/range1198186/1

https://synbiohub.org/public/igem/BBa_I11031/annotation1198186/range1198186/1
type: Range
persistent identity: range1198186
display id: range1198186
version: 1
direction: direction/forward
top level: BBa_I11031
start: 1
end: 348
orientation: inline

AAV
type: SequenceAnnotation
persistent identity: annotation1064115
display id: annotation1064115
version: 1
top level: BBa_I11031
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_I11031/annotation1064115/range1064115/1

https://synbiohub.org/public/igem/BBa_I11031/annotation1064115/range1064115/1
type: Range
persistent identity: range1064115
display id: range1064115
version: 1
direction: direction/forward
top level: BBa_I11031
start: 349
end: 381
orientation: inline

BBa_K112212
type: ComponentDefinition
persistent identity: BBa K112212
display id: BBa_K112212
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: {rbs.int!} The integrase gene with native rbs site and stop codon, BBb format
creator: Molly Allen
created: 2008-10-20T11:00:00Z
modified: 2015-05-08T01:09:15Z
discontinued: false
dominant: false
experience: experience/None
group u list: _224_
m user id: 0
owner id: 2048
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at: [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
mutable notes: ?
mutable provenance: pAH57 plasmid
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
sequence: https://synbiohub.org/public/igem/BBa_K112212_sequence/1

BBa_I11020
type: ComponentDefinition
persistent identity: BBa I11020
display id: BBa_I11020
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: integrase from E. coli phage lambda
creator: bu
created: 2004-09-02T11:00:00Z
modified: 2015-08-31T04:07:30Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2_
m user id: 0
owner id: 102
owning group id: 7
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Wild type lambda integrase
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: stop
sequence annotation: LVA
sequence annotation: Integrase
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_I11020_sequence/1

stop
type: SequenceAnnotation
persistent identity: annotation1064102
display id: annotation1064102
version: 1
top level: BBa_I11020
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_I11020/annotation1064102/range1064102/1

https://synbiohub.org/public/igem/BBa_I11020/annotation1064102/range1064102/1
type: Range
persistent identity: range1064102
display id: range1064102
version: 1
direction: direction/forward
top level: BBa_I11020
start: 1102
end: 1107
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation1064100
display id: annotation1064100
version: 1
top level: BBa_I11020
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_I11020/annotation1064100/range1064100/1

https://synbiohub.org/public/igem/BBa_I11020/annotation1064100/range1064100/1
type: Range
persistent identity: range1064100
display id: range1064100
version: 1
direction: direction/forward
top level: BBa_I11020
start: 1069
end: 1101
orientation: inline

Integrase
type: SequenceAnnotation
persistent identity: annotation1064101
display id: annotation1064101
version: 1
top level: BBa_I11020
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_I11020/annotation1064101/range1064101/1

https://synbiohub.org/public/igem/BBa_I11020/annotation1064101/range1064101/1
type: Range
persistent identity: range1064101
display id: range1064101
version: 1
direction: direction/forward
top level: BBa_I11020
start: 1
end: 1068
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2214027
display id: annotation2214027
version: 1
top level: BBa_I11020
role: SO:0000807
role: feature/barcode
location: https://synbiohub.org/public/igem/BBa_I11020/annotation2214027/range2214027/1

https://synbiohub.org/public/igem/BBa_I11020/annotation2214027/range2214027/1
type: Range
persistent identity: range2214027
display id: range2214027
version: 1
direction: direction/forward
top level: BBa_I11020
start: 1108
end: 1132
orientation: inline

BBa_J70007
type: ComponentDefinition
persistent identity: BBa J70007
display id: BBa_J70007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: recT recombination gene from Spiroplasma citri
creator: Tom Knight
created: 2008-04-07T11:00:00Z
modified: 2015-05-08T01:08:20Z
discontinued: false
dominant: false
group u list: _41_48_1_
m user id: 0
owner id: 6
owning group id: 48
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: recET recombination gene from Spiroplasma citri, probably catalyzing the homologous recombination of DNA fragments similar to the Lambda Bet gene (Lambda RED recombination). This gene is from S. citri, a close relative of Me. florum, and has very similar codon usage.
mutable notes: Eliminate GATC site by silent mutation to GACC (asp -> asp). Note very low GC content at the 5' end. The primers are: * gtttcttcgaattcgcggccgcttctagatgaataataaaattgaattaatattaaataattc,recT-F * gtttcttctactagtagcggccgctgcagttattaatcagaaaacatttcattaacagc,recT-R * gaaatgcaagaagcctatcaacatgaccaagcaataattattaataacagc,recT-mut-F * gctgttattaataattattgcttggtcatgttgataggcttcttgcatttc,recT-mut-R
mutable provenance: Genbank entry CAK99381 protein, nucleotide coding region. Mutations of the single GATC site to avoid restriction by the Sau3AI restriction system in Me. florum. PCR from S. citri DNA, kind gift of Prof. J. Renaudin.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: recT
sequence: https://synbiohub.org/public/igem/BBa_J70007_sequence/1

recT
type: SequenceAnnotation
persistent identity: annotation1983023
display id: annotation1983023
version: 1
top level: BBa_J70007
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J70007/annotation1983023/range1983023/1

https://synbiohub.org/public/igem/BBa_J70007/annotation1983023/range1983023/1
type: Range
persistent identity: range1983023
display id: range1983023
version: 1
direction: direction/forward
top level: BBa_J70007
start: 1
end: 858
orientation: inline

BBa_I716213
type: ComponentDefinition
persistent identity: BBa I716213
display id: BBa_I716213
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Cre (TTG start)
creator: Samantha Liang
created: 2007-06-04T11:00:00Z
modified: 2015-08-31T04:07:51Z
discontinued: false
dominant: true
experience: experience/None
group u list: _110_
m user id: 0
owner id: 933
owning group id: 52
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Cre (TTG start) related to I716212
mutable notes: PCR sil06 and G00101 on pBca9145-Bca1117 (PCR product is 1147bp, BglII/XhoI 1047 bp) Sub into pBca9145-Bca1089 (BglII/XhoI, 2063+696, L) Product is pBca9145-I716213 (TTG start) sil06 Forward BglII Cre with TTG start ctagcAGATCTttgtccaatttactgaccgtac G00101 Reverse Sequencing of pSB1A* plasmids attaccgcctttgagtgagc
mutable provenance: PCR sil06 and G00101 on pBca9145-Bca1117 (PCR product is 1147bp, BglII/XhoI 1047 bp) Sub into pBca9145-Bca1089 (BglII/XhoI, 2063+696, L) Product is pBca9145-I716213 (TTG start)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_I716213_sequence/1

Bxb1_Xis
type: ComponentDefinition
persistent identity: BBa K907001
display id: BBa_K907001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Mycobacterium Phage Bxb1 gp47, DNA excisionase
creator: Dong-hui Choe, Soo-in Lee
created: 2012-09-19T11:00:00Z
modified: 2015-05-08T01:13:44Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1172_
m user id: 0
owner id: 12628
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This part is the protein coding sequence which encodes DNA integrase of Mycobacterium phage Bxb1. The Bxb1 integrase is a DNA recombinase, more precisely a member of serine integrase family. It recognizes specific sequences, called attB and attP, and then integrate, invert, or excise depend on orientations of recognition sequences. We used this integrase to invert specific sequence contained in plasmid. This protein is well expressed in E.coli(strain MG1655). When it inverts DNA, the attB and attP sequences are changed into attL and attR, as common DNA recombinases do. Another protein called Bxb1 excisionase interacts with integrase and that complex flips back inverted DNA into original sequence regenerating attB and attP sequences.
mutable notes: Internal restriction endonuclease site is changed(two PstI sites, one XbaI sites) without changing amino acid sequence.
mutable provenance: This part is derived by Mycobacterium phage Bxb1. And synthesized by Bioneer. http://www.ncbi.nlm.nih.gov/protein/NP_075302.1
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Bacteriophage Bxb1 excisionase CDS
sequence: https://synbiohub.org/public/igem/BBa_K907001_sequence/1

Bacteriophage Bxb1 excisionase CDS
type: SequenceAnnotation
persistent identity: annotation2187532
display id: annotation2187532
version: 1
top level: Bxb1_Xis
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K907001/annotation2187532/range2187532/1

https://synbiohub.org/public/igem/BBa_K907001/annotation2187532/range2187532/1
type: Range
persistent identity: range2187532
display id: range2187532
version: 1
direction: direction/forward
top level: Bxb1_Xis
start: 1
end: 768
orientation: inline

BBa_K1926012
type: ComponentDefinition
persistent identity: BBa K1926012
display id: BBa_K1926012
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: The VIKA UNIT: recombinase VIKA flanked by Vox
creator: Xinyi Liu
created: 2016-10-08T11:00:00Z
modified: 2016-10-11T03:47:23Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2393_
m user id: 28998
owner id: 28998
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This part is the VIKA UNIT of cyclebow system including an sv40 nuclear location sequence (NLS), a recombinase gene named VIKA and an sv40 terminator flanked by homodromous recognition site of recombinase VIKA named Vox. The whole part can be cut off by VIKA. This part is the improvement of the BBa_K1641003，which is a plain VIKA. You may use this part to: 1) Test the cut-off efficiency of recombinase VIKA by qRT-PCR; 2) Fast cut off this part by transient transfecting recombinase gene into nucleus; 3) Do lineage tracking.
mutable notes: None
mutable provenance: The recombinase gene VIKA and its RTS Vox were got through denovo synthesis. NLS and sv40 terminator were got from commercial plasmid: pentry nls GFP/pcdn3.1.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: Vox
sequence annotation: TAA
sequence annotation: VIKA
sequence annotation: sv40 terminator
sequence: https://synbiohub.org/public/igem/BBa_K1926012_sequence/1

Vox
type: SequenceAnnotation
persistent identity: annotation2491854
display id: annotation2491854
version: 1
top level: BBa_K1926012
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1926012/annotation2491854/range2491854/1

https://synbiohub.org/public/igem/BBa_K1926012/annotation2491854/range2491854/1
type: Range
persistent identity: range2491854
display id: range2491854
version: 1
direction: direction/forward
top level: BBa_K1926012
start: 1
end: 34
orientation: inline

Vox
type: SequenceAnnotation
persistent identity: annotation2491856
display id: annotation2491856
version: 1
top level: BBa_K1926012
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1926012/annotation2491856/range2491856/1

https://synbiohub.org/public/igem/BBa_K1926012/annotation2491856/range2491856/1
type: Range
persistent identity: range2491856
display id: range2491856
version: 1
direction: direction/forward
top level: BBa_K1926012
start: 1261
end: 1294
orientation: inline

TAA
type: SequenceAnnotation
persistent identity: annotation2491858
display id: annotation2491858
version: 1
top level: BBa_K1926012
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1926012/annotation2491858/range2491858/1

https://synbiohub.org/public/igem/BBa_K1926012/annotation2491858/range2491858/1
type: Range
persistent identity: range2491858
display id: range2491858
version: 1
direction: direction/forward
top level: BBa_K1926012
start: 1139
end: 1141
orientation: inline

VIKA
type: SequenceAnnotation
persistent identity: annotation2491855
display id: annotation2491855
version: 1
top level: BBa_K1926012
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1926012/annotation2491855/range2491855/1

https://synbiohub.org/public/igem/BBa_K1926012/annotation2491855/range2491855/1
type: Range
persistent identity: range2491855
display id: range2491855
version: 1
direction: direction/forward
top level: BBa_K1926012
start: 35
end: 1117
orientation: inline

sv40 terminator
type: SequenceAnnotation
persistent identity: annotation2491857
display id: annotation2491857
version: 1
top level: BBa_K1926012
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1926012/annotation2491857/range2491857/1

https://synbiohub.org/public/igem/BBa_K1926012/annotation2491857/range2491857/1
type: Range
persistent identity: range2491857
display id: range2491857
version: 1
direction: direction/forward
top level: BBa_K1926012
start: 1118
end: 1260
orientation: inline

BBa_I11021
type: ComponentDefinition
persistent identity: BBa I11021
display id: BBa_I11021
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: excisionase from E. coli phage lambda (removes prophage from host genome)
creator: mschomp
created: 2004-09-02T11:00:00Z
modified: 2015-08-31T04:07:30Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2_
m user id: 0
owner id: 102
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Lambda excisionase coding region with AAV degradation tag and two stop codons
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: stop
sequence annotation: cI lambda
sequence annotation: AAV
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_I11021_sequence/1

stop
type: SequenceAnnotation
persistent identity: annotation1064104
display id: annotation1064104
version: 1
top level: BBa_I11021
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_I11021/annotation1064104/range1064104/1

https://synbiohub.org/public/igem/BBa_I11021/annotation1064104/range1064104/1
type: Range
persistent identity: range1064104
display id: range1064104
version: 1
direction: direction/forward
top level: BBa_I11021
start: 250
end: 256
orientation: inline

cI lambda
type: SequenceAnnotation
persistent identity: annotation1064105
display id: annotation1064105
version: 1
top level: BBa_I11021
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_I11021/annotation1064105/range1064105/1

https://synbiohub.org/public/igem/BBa_I11021/annotation1064105/range1064105/1
type: Range
persistent identity: range1064105
display id: range1064105
version: 1
direction: direction/forward
top level: BBa_I11021
start: 1
end: 212
orientation: inline

AAV
type: SequenceAnnotation
persistent identity: annotation1064103
display id: annotation1064103
version: 1
top level: BBa_I11021
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_I11021/annotation1064103/range1064103/1

https://synbiohub.org/public/igem/BBa_I11021/annotation1064103/range1064103/1
type: Range
persistent identity: range1064103
display id: range1064103
version: 1
direction: direction/forward
top level: BBa_I11021
start: 213
end: 249
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2214029
display id: annotation2214029
version: 1
top level: BBa_I11021
role: SO:0000807
role: feature/barcode
location: https://synbiohub.org/public/igem/BBa_I11021/annotation2214029/range2214029/1

https://synbiohub.org/public/igem/BBa_I11021/annotation2214029/range2214029/1
type: Range
persistent identity: range2214029
display id: range2214029
version: 1
direction: direction/forward
top level: BBa_I11021
start: 256
end: 280
orientation: inline

BBa_K112207
type: ComponentDefinition
persistent identity: BBa K112207
display id: BBa_K112207
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: {int!} The integrase gene with start and stop codon, BBb format
creator: Molly Allen
created: 2008-10-20T11:00:00Z
modified: 2015-05-08T01:09:14Z
discontinued: false
dominant: false
experience: experience/None
group u list: _224_
m user id: 0
owner id: 2048
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at: [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
mutable notes: ?
mutable provenance: pAH57 plasmid
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K112207_sequence/1

ihfB
type: ComponentDefinition
persistent identity: BBa K112224
display id: BBa_K112224
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: {rbs.ihfB!} The ihf beta gene with native rbs and stop codon, BBb format
creator: Molly Allen
created: 2008-10-20T11:00:00Z
modified: 2015-05-08T01:09:15Z
discontinued: false
dominant: false
experience: experience/None
group u list: _224_
m user id: 0
owner id: 2048
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at: [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
mutable notes: ?
mutable provenance: MG1655
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
sequence: https://synbiohub.org/public/igem/BBa_K112224_sequence/1

ihfA
type: ComponentDefinition
persistent identity: BBa K112218
display id: BBa_K112218
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: {rbs.ihfA!} The ihf alpha gene with native rbs and stop codon, BBb format
creator: Molly Allen
created: 2008-10-20T11:00:00Z
modified: 2015-05-08T01:09:15Z
discontinued: false
dominant: false
experience: experience/None
group u list: _224_
m user id: 0
owner id: 2048
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at: [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
mutable notes: ?
mutable provenance: MG1655
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
sequence: https://synbiohub.org/public/igem/BBa_K112218_sequence/1

BBa_K112209
type: ComponentDefinition
persistent identity: BBa K112209
display id: BBa_K112209
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: {int>} The integrase gene without a stop codon, BBb format
creator: Molly Allen
created: 2008-10-20T11:00:00Z
modified: 2015-05-08T01:09:14Z
discontinued: false
dominant: false
experience: experience/None
group u list: _224_
m user id: 0
owner id: 2048
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at: [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
mutable notes: ?
mutable provenance: pAH57 plasmid
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence: https://synbiohub.org/public/igem/BBa_K112209_sequence/1

Bxb1_Int
type: ComponentDefinition
persistent identity: BBa K907000
display id: BBa_K907000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Mycobacterium Phage Bxb1 gp35, DNA integrase
creator: Dong-hui Choe, Soo-in Lee
created: 2012-09-19T11:00:00Z
modified: 2015-05-08T01:13:44Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1172_
m user id: 0
owner id: 12628
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: This part is the protein coding sequence which encodes DNA integrase of Mycobacterium phage Bxb1. The Bxb1 integrase is a DNA recombinase, more precisely a member of serine integrase family. It recognizes specific sequences, called attB and attP, and then integrate, invert, or excise depend on orientations of recognition sequences. We used this integrase to invert specific sequence contained in plasmid. This protein is well expressed in E.coli(strain MG1655). When it inverts DNA, the attB and attP sequences are changed into attL and attR, as common DNA recombinases do. Another protein called Bxb1 excisionase interacts with integrase and that complex flips back inverted DNA into original sequence regenerating attB and attP sequences.
mutable notes: Internal restriction endonuclease site is changed(two PstI sites, one XbaI sites) without changing amino acid sequence.
mutable provenance: This part is derived by Mycobacterium phage Bxb1. And synthesized by Bioneer. http://www.ncbi.nlm.nih.gov/protein/NP_075302.1
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Mycobacterium phage Bxb1 integrase CDS
sequence: https://synbiohub.org/public/igem/BBa_K907000_sequence/1

Mycobacterium phage Bxb1 integrase CDS
type: SequenceAnnotation
persistent identity: annotation2187533
display id: annotation2187533
version: 1
top level: Bxb1_Int
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K907000/annotation2187533/range2187533/1

https://synbiohub.org/public/igem/BBa_K907000/annotation2187533/range2187533/1
type: Range
persistent identity: range2187533
display id: range2187533
version: 1
direction: direction/forward
top level: Bxb1_Int
start: 1
end: 1503
orientation: inline

BBa_K1015999
type: ComponentDefinition
persistent identity: BBa K1015999
display id: BBa_K1015999
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: lacA-homology sequence(for RED recombination system)
creator: Kento Tominaga
created: 2013-08-27T11:00:00Z
modified: 2015-05-08T01:08:43Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1321_
m user id: 0
owner id: 15618
owning group id: 9
sample status: No part sequence
status: status/Deleted
bookmark: false
mutable description: Flippase
mutable notes: ATGCCACAATTTGGTATATTATGTAAAACACCACCTAAGGTGCTTGTTCGTCAGTTTGTGGAAAGGTTTGAAAGACCTTCAGGTGAGAAAATAGCATTATGTGCTGCTGAACTAACCTATTTATGTTGGATGATTACACATAACGGAACAGCAATCAAGAGAGCCACATTCATGAGCTATAATACTATCATAAGCAATTCGCTGAGTTTCGATATTGTCAATAAATCACTCCAGTTTAAATACAAGACGCAAAAAGCAACAATTCTGGAAGCCTCATTAAAGAAATTGATTCCTGCTTGGGAATTTACAATTATTCCTTACTATGGACAAAAACATCAATCTGATATCACTGATATTGTAAGTAGTTTGCAATTACAGTTCGAATCATCGGAAGAAGCAGATAAGGGAAATAGCCACAGTAAAAAAATGCTTAAAGCACTTCTAAGTGAGGGTGAAAGCATCTGGGAGATCACTGAGAAAATACTAAATTCGTTTGAGTATACTTCGAGATTTACAAAAACAAAAACTTTATACCAATTCCTCTTCCTAGCTACTTTCATCAATTGTGGAAGATTCAGCGATATTAAGAACGTTGATCCGAAATCATTTAAATTAGTCCAAAATAAGTATCTGGGAGTAATAATCCAGTGTTTAGTGACAGAGACAAAGACAAGCGTTAGTAGGCACATATACTTCTTTAGCGCAAGGGGTAGGATCGATCCACTTGTATATTTGGATGAATTTTTGAGGAATTCTGAACCAGTCCTAAAACGAGTAAATAGGACCGGCAATTCTTCAAGCAATAAACAGGAATACCAATTATTAAAAGATAACTTAGTCAGATCGTACAATAAAGCTTTGAAGAAAAATGCGCCTTATTCAATCTTTGCTATAAAAAATGGCCCAAAATCTCACATTGGAAGACATTTGATGACCTCATTTCTTTCAATGAAGGGCCTAACGGAGTTGACTAATGTTGTGGGAAATTGGAGCGATAAGCGTGCTTCTGCCGTGGCCAGGACAACGTATACTCATCAGATAACAGCAATACCTGATCACTACTTCGCACTAGTTTCTCGGTACTATGCATATGATCCAATATCAAAGGAAATGATAGCATTGAAGGATGAGACTAATCCAATTGAGGAGTGGCAGCATATAGAACAGCTAAAGGGTAGTGCTGAAGGAAGCATACGATACCCCGCATGGAATGGGATAATATCACAGGAGGTACTAGACTACCTTTCATCCTACATAAATAGACGCATATAA
mutable provenance: Saccharomyces cerevisiae 2 micron circle plasmid
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: BBa_K1015000
sequence annotation: lacA homologous sequence

BBa_K1015000
type: SequenceAnnotation
persistent identity: annotation2342546
display id: annotation2342546
version: 1
top level: BBa_K1015999
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1015999/annotation2342546/range2342546/1

https://synbiohub.org/public/igem/BBa_K1015999/annotation2342546/range2342546/1
type: Range
persistent identity: range2342546
display id: range2342546
version: 1
direction: direction/forward
top level: BBa_K1015999
start: 1
end: 36
orientation: inline

lacA homologous sequence
type: SequenceAnnotation
persistent identity: annotation2342545
display id: annotation2342545
version: 1
top level: BBa_K1015999
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1015999/annotation2342545/range2342545/1

https://synbiohub.org/public/igem/BBa_K1015999/annotation2342545/range2342545/1
type: Range
persistent identity: range2342545
display id: range2342545
version: 1
direction: direction/forward
top level: BBa_K1015999
start: 1
end: 36
orientation: inline

BBa_K112245
type: ComponentDefinition
persistent identity: BBa K112245
display id: BBa_K112245
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: {pAH57 promoter}{rbs.xis.int!} inserted into K112995 plasmid
creator: Molly Allen
created: 2008-10-28T12:00:00Z
modified: 2015-06-15T01:11:26Z
discontinued: true
dominant: false
experience: experience/Works
group u list: _224_
m user id: 4206
owner id: 2048
owning group id: 9
sample status: It's complicated
status: status/Deleted
bookmark: true
mutable description: This part was inserted into the K112995 plasmid between ccdB and the attR2 site. This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at: [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
mutable notes: ?
mutable provenance: pAH57 Plasmid
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000155
role: partType/Plasmid
sequence annotation: rbs.xis.int!
sequence annotation: ccdB
sequence annotation: attR1
sequence annotation: attR2
sequence annotation: pAH57 promoter
sequence: https://synbiohub.org/public/igem/BBa_K112245_sequence/1

rbs.xis.int!
type: SequenceAnnotation
persistent identity: annotation1993905
display id: annotation1993905
version: 1
top level: BBa_K112245
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K112245/annotation1993905/range1993905/1

https://synbiohub.org/public/igem/BBa_K112245/annotation1993905/range1993905/1
type: Range
persistent identity: range1993905
display id: range1993905
version: 1
direction: direction/forward
top level: BBa_K112245
start: 1776
end: 3061
orientation: inline

ccdB
type: SequenceAnnotation
persistent identity: annotation1993903
display id: annotation1993903
version: 1
top level: BBa_K112245
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K112245/annotation1993903/range1993903/1

https://synbiohub.org/public/igem/BBa_K112245/annotation1993903/range1993903/1
type: Range
persistent identity: range1993903
display id: range1993903
version: 1
direction: direction/forward
top level: BBa_K112245
start: 640
end: 849
orientation: inline

attR1
type: SequenceAnnotation
persistent identity: annotation1993902
display id: annotation1993902
version: 1
top level: BBa_K112245
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K112245/annotation1993902/range1993902/1

https://synbiohub.org/public/igem/BBa_K112245/annotation1993902/range1993902/1
type: Range
persistent identity: range1993902
display id: range1993902
version: 1
direction: direction/forward
top level: BBa_K112245
start: 16
end: 140
orientation: inline

attR2
type: SequenceAnnotation
persistent identity: annotation1993929
display id: annotation1993929
version: 1
top level: BBa_K112245
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K112245/annotation1993929/range1993929/1

https://synbiohub.org/public/igem/BBa_K112245/annotation1993929/range1993929/1
type: Range
persistent identity: range1993929
display id: range1993929
version: 1
direction: direction/forward
top level: BBa_K112245
start: 3074
end: 3198
orientation: inline

pAH57 promoter
type: SequenceAnnotation
persistent identity: annotation1993904
display id: annotation1993904
version: 1
top level: BBa_K112245
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K112245/annotation1993904/range1993904/1

https://synbiohub.org/public/igem/BBa_K112245/annotation1993904/range1993904/1
type: Range
persistent identity: range1993904
display id: range1993904
version: 1
direction: direction/forward
top level: BBa_K112245
start: 893
end: 1769
orientation: inline

BBa_K112215
type: ComponentDefinition
persistent identity: BBa K112215
display id: BBa_K112215
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: {ihfA>} The ihf alpha gene without a stop codon, BBb format
creator: Molly Allen
created: 2008-10-20T11:00:00Z
modified: 2015-05-08T01:09:15Z
discontinued: false
dominant: false
experience: experience/None
group u list: _224_
m user id: 0
owner id: 2048
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at: [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
mutable notes: ?
mutable provenance: MG1655
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence: https://synbiohub.org/public/igem/BBa_K112215_sequence/1

BBa_K112211
type: ComponentDefinition
persistent identity: BBa K112211
display id: BBa_K112211
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: {a~int!} The integrase gene with rbs ready to be attached with stop codon, BBb format
creator: Molly Allen
created: 2008-10-20T11:00:00Z
modified: 2015-05-08T01:09:15Z
discontinued: false
dominant: false
experience: experience/None
group u list: _224_
m user id: 0
owner id: 2048
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at: [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
mutable notes: ?
mutable provenance: pAH57 plasmid
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K112211_sequence/1

BBa_K112217
type: ComponentDefinition
persistent identity: BBa K112217
display id: BBa_K112217
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: {a~ihfA!} The ihf alpha gene ready to have rbs attached with stop codon, BBb format
creator: Molly Allen
created: 2008-10-20T11:00:00Z
modified: 2015-05-08T01:09:15Z
discontinued: false
dominant: false
experience: experience/None
group u list: _224_
m user id: 0
owner id: 2048
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at: [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
mutable notes: ?
mutable provenance: MG1655
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K112217_sequence/1

BBa_K108022
type: ComponentDefinition
persistent identity: BBa K108022
display id: BBa_K108022
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RuvA
creator: Qi Liu
created: 2008-10-22T11:00:00Z
modified: 2015-05-08T01:09:04Z
discontinued: false
dominant: false
experience: experience/None
group u list: _222_
m user id: 0
owner id: 2671
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: component of RuvABC resolvasome, regulatory subunit
mutable notes: PCR using primers with biobrick prefix and surfix, and then cloned into pSB1AC3.
mutable provenance: E.coli K12 DH10B genome
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: RuvA
sequence: https://synbiohub.org/public/igem/BBa_K108022_sequence/1

RuvA
type: SequenceAnnotation
persistent identity: annotation1986977
display id: annotation1986977
version: 1
top level: BBa_K108022
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K108022/annotation1986977/range1986977/1

https://synbiohub.org/public/igem/BBa_K108022/annotation1986977/range1986977/1
type: Range
persistent identity: range1986977
display id: range1986977
version: 1
direction: direction/forward
top level: BBa_K108022
start: 1
end: 609
orientation: inline

BBa_K112101
type: ComponentDefinition
persistent identity: BBa K112101
display id: BBa_K112101
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Cre enzyme without stop codon
creator: Madhvi Venkatesh
created: 2008-10-20T11:00:00Z
modified: 2015-05-08T01:09:13Z
discontinued: false
dominant: false
experience: experience/None
group u list: _224_
m user id: 0
owner id: 3023
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: Cre enzyme without stop codon
mutable notes: This part is built in Biobrick version 2.0. In this format, parts are flanked by BglII and BamHI restriction sites. --------------------------------------------- PCR mv003/mv004 on pBca9145-Bca1010 (1060bp, EcoRI/BamHI/DpnI) Sub into pBca1256 (EcoRI/BamHI, 2472+910, L) Product is pBca1256-K112101 ----------------------------------------------- mv003 Forward Biobricking of Cre> ccataGAATTCatgAGATCTatgtccaatttactgaccgtac mv004 Reverse Biobricking of Cre> cgttaGGATCCatcgccatcttccagcaggcg <//pre>
mutable provenance: PCR mv003/mv004 on pBca9145-Bca1010 (1060bp, EcoRI/BamHI/DpnI) Sub into pBca1256 (EcoRI/BamHI, 2472+910, L) Product is pBca1256-K112101
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence: https://synbiohub.org/public/igem/BBa_K112101_sequence/1

BBa_K112100
type: ComponentDefinition
persistent identity: BBa K112100
display id: BBa_K112100
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Cre enzyme without start codon
creator: Madhvi Venkatesh
created: 2008-08-13T11:00:00Z
modified: 2015-05-08T01:09:13Z
discontinued: false
dominant: false
experience: experience/None
group u list: _224_
m user id: 0
owner id: 3023
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: Cre enzyme without start codon
mutable notes: This part is built in Biobrick version 2.0. In this format, parts are flanked by BglII and BamHI restriction sites. PCR mv001/mv002 on pBca9145-Bca1010 (1060bp, EcoRI/BamHI/DpnI) Sub into pBca1256 (EcoRI/BamHI, 2472+910, L) Product is pBca1256-K112100 ----------------------------------------------- mv001 Forward Biobricking of <Cre! ccataGAATTCatgAGATCTtccaatttactgaccgtacacc mv002 Reverse Biobricking of <Cre! cgttaGGATCCttaatcgccatcttccagc
mutable provenance: PCR mv001/mv002 on pBca9145-Bca1010 (1060bp, EcoRI/BamHI) Sub into pBca1256 (EcoRI/BamHI, 2472+910, L) Product is pBca1256-K112100
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence: https://synbiohub.org/public/igem/BBa_K112100_sequence/1

BBa_K112208
type: ComponentDefinition
persistent identity: BBa K112208
display id: BBa_K112208
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: {< int!} The integrase gene without a start codon, BBb format
creator: Molly Allen
created: 2008-10-20T11:00:00Z
modified: 2015-05-08T01:09:14Z
discontinued: false
dominant: false
experience: experience/None
group u list: _224_
m user id: 0
owner id: 2048
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at: [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
mutable notes: ?
mutable provenance: pAH57 plasmid
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence: https://synbiohub.org/public/igem/BBa_K112208_sequence/1

BBa_K112216
type: ComponentDefinition
persistent identity: BBa K112216
display id: BBa_K112216
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: {< ihfA >} The ihf alpha gene without start and stop codons, BBb format
creator: Molly Allen
created: 2008-10-20T11:00:00Z
modified: 2015-05-08T01:09:15Z
discontinued: false
dominant: false
experience: experience/None
group u list: _224_
m user id: 0
owner id: 2048
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at: [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
mutable notes: ?
mutable provenance: MG1655
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence: https://synbiohub.org/public/igem/BBa_K112216_sequence/1

BBa_J61030
type: ComponentDefinition
persistent identity: BBa J61030
display id: BBa_J61030
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: [OriTr][R6K]
creator: John Anderson
created: 2006-09-20T11:00:00Z
modified: 2015-08-31T02:02:59Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Intermediate of the oriTr (RP4 origin of transfer) and R6K (conditional origin of replication) pSB1A2-Bca9009
mutable notes: N/A
mutable provenance: standard biobrick assembly
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_J01003
component: BBa_J61001
sequence annotation: BBa_J61001
sequence annotation: BBa_J01003
sequence: https://synbiohub.org/public/igem/BBa_J61030_sequence/1

BBa_J01003
type: Component
persistent identity: component1902257
display id: component1902257
version: 1
top level: BBa_J61030
access: public
definition: https://synbiohub.org/public/igem/BBa_J01003/1

BBa_J61001
type: Component
persistent identity: component1902259
display id: component1902259
version: 1
top level: BBa_J61030
access: public
definition: https://synbiohub.org/public/igem/BBa_J61001/1

BBa_J61001
type: SequenceAnnotation
persistent identity: annotation1902259
display id: annotation1902259
version: 1
top level: BBa_J61030
location: https://synbiohub.org/public/igem/BBa_J61030/annotation1902259/range1902259/1
component: BBa_J61001

https://synbiohub.org/public/igem/BBa_J61030/annotation1902259/range1902259/1
type: Range
persistent identity: range1902259
display id: range1902259
version: 1
direction: direction/reverse
top level: BBa_J61030
start: 380
end: 785
orientation: inline

BBa_J01003
type: SequenceAnnotation
persistent identity: annotation1902257
display id: annotation1902257
version: 1
top level: BBa_J61030
location: https://synbiohub.org/public/igem/BBa_J61030/annotation1902257/range1902257/1
component: BBa_J01003

https://synbiohub.org/public/igem/BBa_J61030/annotation1902257/range1902257/1
type: Range
persistent identity: range1902257
display id: range1902257
version: 1
direction: direction/reverse
top level: BBa_J61030
start: 1
end: 371
orientation: inline

BBa_K112222
type: ComponentDefinition
persistent identity: BBa K112222
display id: BBa_K112222
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: {< ihfB >} The ihf beta gene without start or stop codons, BBb format
creator: Molly Allen
created: 2008-10-20T11:00:00Z
modified: 2015-05-08T01:09:15Z
discontinued: false
dominant: false
experience: experience/None
group u list: _224_
m user id: 0
owner id: 2048
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at: [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
mutable notes: ?
mutable provenance: MG1655
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence: https://synbiohub.org/public/igem/BBa_K112222_sequence/1

BBa_K112214
type: ComponentDefinition
persistent identity: BBa K112214
display id: BBa_K112214
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: {< ihfA!} The ihf alpha gene without a start codon, BBb format
creator: Molly Allen
created: 2008-10-20T11:00:00Z
modified: 2015-05-08T01:09:15Z
discontinued: false
dominant: false
experience: experience/None
group u list: _224_
m user id: 0
owner id: 2048
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at: [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
mutable notes: ?
mutable provenance: MG1655
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence: https://synbiohub.org/public/igem/BBa_K112214_sequence/1

BBa_K1926021
type: ComponentDefinition
persistent identity: BBa K1926021
display id: BBa_K1926021
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: The SNAP-VIKA UNIT
creator: Xinyi Liu
created: 2016-10-09T11:00:00Z
modified: 2016-10-11T06:21:46Z
discontinued: false
dominant: false
group u list: _2393_
m user id: 28998
owner id: 28998
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This part is the SNAP-VIKA UNIT of cyclebow including a SNAP UNIT and a VIKA UNIT. The SNAP UNIT, which can be cut off by CRE, includes an sv40 nuclear location sequence (NLS), a covalent labeling of fusion proteins named SNAP-tag?? and an sv40 terminator flanked by homodromous recognition site of recombinase CRE named loxP. The VIKA UNIT, which can be cut off by VIKA, includes an sv40 nuclear location sequence (NLS), a recombinase gene named VIKA and an sv40 terminator flanked by homodromous recognition site of recombinase VIKA named Vox. You may use this part to: 1) Test the cut-off efficiency of recombinase CRE and VIKA by qRT-PCR; 2) Estimate the time needed for genome repair because VIKA can only be expressed after the cut off of SNAP UNIT and genome repair; 3) Fast cut off this part by transient transfecting recombinase gene into nucleus; 4) When there is no CRE, you can use this part as the SNAP UNIT, which can help localize proteins in vivo with BLOCK and dye from NEB company.
mutable notes: None
mutable provenance: The SNAP-tag?? was brought from NEB company. NLS and sv40 terminator were got from commercial plasmid: pentry nls GFP/pcdna3.1. The recombinase gene VIKA and its RTS Vox were got through denovo synthesis.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1926012
component: BBa_K1926011
sequence annotation: BBa_K1926011
sequence annotation: BBa_K1926012
sequence: https://synbiohub.org/public/igem/BBa_K1926021_sequence/1

BBa_K1926012
type: Component
persistent identity: component2491919
display id: component2491919
version: 1
top level: BBa_K1926021
access: public
definition: BBa_K1926012

BBa_K1926011
type: Component
persistent identity: component2491913
display id: component2491913
version: 1
top level: BBa_K1926021
access: public
definition: https://synbiohub.org/public/igem/BBa_K1926011/1

BBa_K1926011
type: SequenceAnnotation
persistent identity: annotation2491913
display id: annotation2491913
version: 1
top level: BBa_K1926021
location: https://synbiohub.org/public/igem/BBa_K1926021/annotation2491913/range2491913/1
component: BBa_K1926011

https://synbiohub.org/public/igem/BBa_K1926021/annotation2491913/range2491913/1
type: Range
persistent identity: range2491913
display id: range2491913
version: 1
direction: direction/reverse
top level: BBa_K1926021
start: 1
end: 788
orientation: inline

BBa_K1926012
type: SequenceAnnotation
persistent identity: annotation2491919
display id: annotation2491919
version: 1
top level: BBa_K1926021
location: https://synbiohub.org/public/igem/BBa_K1926021/annotation2491919/range2491919/1
component: BBa_K1926012

https://synbiohub.org/public/igem/BBa_K1926021/annotation2491919/range2491919/1
type: Range
persistent identity: range2491919
display id: range2491919
version: 1
direction: direction/reverse
top level: BBa_K1926021
start: 789
end: 2082
orientation: inline

BBa_K112221
type: ComponentDefinition
persistent identity: BBa K112221
display id: BBa_K112221
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: {ihfB>} The ihf beta gene without a stop codon, BBb format
creator: Molly Allen
created: 2008-10-20T11:00:00Z
modified: 2015-05-08T01:09:15Z
discontinued: false
dominant: false
experience: experience/None
group u list: _224_
m user id: 0
owner id: 2048
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at: [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
mutable notes: ?
mutable provenance: MG1655
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence: https://synbiohub.org/public/igem/BBa_K112221_sequence/1

BBa_K108023
type: ComponentDefinition
persistent identity: BBa K108023
display id: BBa_K108023
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RuvB
creator: Qi Liu
created: 2008-10-22T11:00:00Z
modified: 2015-05-08T01:09:04Z
discontinued: false
dominant: true
experience: experience/None
group u list: _222_
m user id: 0
owner id: 2671
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: ATP-dependent DNA helicase, component of RuvABC resolvasome
mutable notes: PCR using primers with biobrick prefix and surfix, and then cloned into pSB1AC3.
mutable provenance: E.coli K12 DH10B genome
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: RuvB
sequence: https://synbiohub.org/public/igem/BBa_K108023_sequence/1

RuvB
type: SequenceAnnotation
persistent identity: annotation1986978
display id: annotation1986978
version: 1
top level: BBa_K108023
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K108023/annotation1986978/range1986978/1

https://synbiohub.org/public/igem/BBa_K108023/annotation1986978/range1986978/1
type: Range
persistent identity: range1986978
display id: range1986978
version: 1
direction: direction/forward
top level: BBa_K108023
start: 1
end: 1008
orientation: inline

Rci
type: ComponentDefinition
persistent identity: BBa K361000
display id: BBa_K361000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Rci site-specific recombinase
creator: Jacky, Fong Chuen, Loo
created: 2010-10-23T11:00:00Z
modified: 2015-05-08T01:12:12Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _476_
m user id: 0
owner id: 6123
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This part will prouce a 384-amino acid Rci site-specific recombinase used in shufflon system, which is a site-specific DNA recombination between two specific repeat sites as recognition. After this recombinase is being expressed, it can alter the DNA sequence being inserted randomly with the specific repeat sites originally to become an unknown sequence.
mutable notes: The gene is come from muliple inversion systems in R64 plasmid and there is no further modification. Reports have shown that some modification of the gene will lead to deletion some parts of DNA sequence during recombination process.
mutable provenance: It belongs to integrase family of site-specific recombinase, and it is extracted from muliple inversion systems in R64 plasmid, found in genomic sequence code:AP005147.1
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Rci recombinase
sequence: https://synbiohub.org/public/igem/BBa_K361000_sequence/1

Rci recombinase
type: SequenceAnnotation
persistent identity: annotation2095181
display id: annotation2095181
version: 1
top level: Rci
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K361000/annotation2095181/range2095181/1

https://synbiohub.org/public/igem/BBa_K361000/annotation2095181/range2095181/1
type: Range
persistent identity: range2095181
display id: range2095181
version: 1
direction: direction/forward
top level: Rci
start: 1
end: 1155
orientation: inline

BBa_I716212
type: ComponentDefinition
persistent identity: BBa I716212
display id: BBa_I716212
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Cre (GTG start)
creator: Samantha Liang
created: 2007-06-04T11:00:00Z
modified: 2015-08-31T04:07:51Z
discontinued: false
dominant: true
experience: experience/None
group u list: _110_
m user id: 0
owner id: 933
owning group id: 52
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: weakened version of Cre (has a GTG start codon) because we want a lower copy
mutable notes: PCR sil05 and G00101 on pBca9145-Bca1117 (PCR product is 1147bp, BglII/XhoI 1047 bp) Sub into pBca9145-Bca1089 (BglII/XhoI, 2063+696, L) Product is pBca9145-I716212 (GTG start) sil05 Forward BglII Cre with GTG start ctagcAGATCTgtgtccaatttactgaccgtac G00101 Reverse Sequencing of pSB1A* plasmids attaccgcctttgagtgagc
mutable provenance: PCR sil05 and G00101 on pBca9145-Bca1117 (PCR product is 1147bp, BglII/XhoI 1047 bp) Sub into pBca9145-Bca1089 (BglII/XhoI, 2063+696, L) Product is pBca9145-I716212 (GTG start) sil05 Forward BglII Cre with GTG start ctagcAGATCTgtgtccaatttactgaccgtac G00101 Reverse Sequencing of pSB1A* plasmids attaccgcctttgagtgagc
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_I716212_sequence/1

BBa_K112122
type: ComponentDefinition
persistent identity: BBa K112122
display id: BBa_K112122
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Cre with stop codon
creator: Christina Brown
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:09:14Z
discontinued: false
dominant: false
group u list: _224_
m user id: 0
owner id: 3241
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at: [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page] <!-- Add more about the biology of this part here
mutable notes: none
mutable provenance: cloned from E. coli MG1655 genome
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K112122_sequence/1

BBa_K863201
type: ComponentDefinition
persistent identity: BBa K863201
display id: BBa_K863201
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: 3' UTR site of alcohol oxidase 1 gene (aox1)
creator: Julia Schirmacher
created: 2012-09-22T11:00:00Z
modified: 2015-05-08T01:13:36Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1123_
m user id: 0
owner id: 13076
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: 3' UTR site of alcohol oxidase 1 gene (AOX1) from K. pastoris used as a recombination site.
mutable notes: primer sequences are coming soon
mutable provenance: The DNA sequence from plasmid pPIC9K of Invitrogen (http://products.invitrogen.com/ivgn/product/V17520?ICID==%3Dsearch-product) was used for primer design. But the genomic DNA of Pichia pastoris wildtype X33 was used for amplification via PCR.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_K863201_sequence/1

BBa_S04524
type: ComponentDefinition
persistent identity: BBa S04524
display id: BBa_S04524
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: K361000:B0014
creator: Jacky, Fong Chuen, Loo
created: 2010-10-23T11:00:00Z
modified: 2015-05-08T01:14:41Z
discontinued: false
dominant: false
experience: experience/None
group u list: _9_
m user id: 0
owner id: 6123
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_K361000
component: BBa_B0014
sequence annotation: BBa_K361000
sequence annotation: BBa_B0014
sequence: https://synbiohub.org/public/igem/BBa_S04524_sequence/1

BBa_K361000
type: Component
persistent identity: component2095183
display id: component2095183
version: 1
top level: BBa_S04524
access: public
definition: Rci

BBa_B0014
type: Component
persistent identity: component2095190
display id: component2095190
version: 1
top level: BBa_S04524
access: public
definition: https://synbiohub.org/public/igem/BBa_B0014/1

BBa_K361000
type: SequenceAnnotation
persistent identity: annotation2095183
display id: annotation2095183
version: 1
top level: BBa_S04524
location: https://synbiohub.org/public/igem/BBa_S04524/annotation2095183/range2095183/1
component: BBa_K361000

https://synbiohub.org/public/igem/BBa_S04524/annotation2095183/range2095183/1
type: Range
persistent identity: range2095183
display id: range2095183
version: 1
direction: direction/reverse
top level: BBa_S04524
start: 1
end: 1155
orientation: inline

BBa_B0014
type: SequenceAnnotation
persistent identity: annotation2095190
display id: annotation2095190
version: 1
top level: BBa_S04524
location: https://synbiohub.org/public/igem/BBa_S04524/annotation2095190/range2095190/1
component: BBa_B0014

https://synbiohub.org/public/igem/BBa_S04524/annotation2095190/range2095190/1
type: Range
persistent identity: range2095190
display id: range2095190
version: 1
direction: direction/reverse
top level: BBa_S04524
start: 1164
end: 1258
orientation: inline

BBa_K112210
type: ComponentDefinition
persistent identity: BBa K112210
display id: BBa_K112210
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: {< int >} The integrase gene without start and stop codons, BBb format
creator: Molly Allen
created: 2008-10-20T11:00:00Z
modified: 2015-05-08T01:09:15Z
discontinued: false
dominant: false
experience: experience/None
group u list: _224_
m user id: 0
owner id: 2048
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at: [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
mutable notes: ?
mutable provenance: pAH57 plasmid
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence: https://synbiohub.org/public/igem/BBa_K112210_sequence/1

BBa_I11030
type: ComponentDefinition
persistent identity: BBa I11030
display id: BBa_I11030
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: integrase from E. coli phage P22
creator: mschomp
created: 2004-09-02T11:00:00Z
modified: 2015-08-31T04:07:30Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2_
m user id: 0
owner id: 102
owning group id: 7
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: P22 Integrase with LVA tag
mutable provenance: Nuc Acid Res, 2001; 29(11):2205-2216
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: LVA
sequence annotation: Help:Barcodes
sequence annotation: Integrase p22
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_I11030_sequence/1

LVA
type: SequenceAnnotation
persistent identity: annotation1126751
display id: annotation1126751
version: 1
top level: BBa_I11030
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_I11030/annotation1126751/range1126751/1

https://synbiohub.org/public/igem/BBa_I11030/annotation1126751/range1126751/1
type: Range
persistent identity: range1126751
display id: range1126751
version: 1
direction: direction/forward
top level: BBa_I11030
start: 1162
end: 1194
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2214028
display id: annotation2214028
version: 1
top level: BBa_I11030
role: SO:0000807
role: feature/barcode
location: https://synbiohub.org/public/igem/BBa_I11030/annotation2214028/range2214028/1

https://synbiohub.org/public/igem/BBa_I11030/annotation2214028/range2214028/1
type: Range
persistent identity: range2214028
display id: range2214028
version: 1
direction: direction/forward
top level: BBa_I11030
start: 1201
end: 1225
orientation: inline

Integrase p22
type: SequenceAnnotation
persistent identity: annotation1126718
display id: annotation1126718
version: 1
top level: BBa_I11030
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_I11030/annotation1126718/range1126718/1

https://synbiohub.org/public/igem/BBa_I11030/annotation1126718/range1126718/1
type: Range
persistent identity: range1126718
display id: range1126718
version: 1
direction: direction/forward
top level: BBa_I11030
start: 1
end: 1161
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1126806
display id: annotation1126806
version: 1
top level: BBa_I11030
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_I11030/annotation1126806/range1126806/1

https://synbiohub.org/public/igem/BBa_I11030/annotation1126806/range1126806/1
type: Range
persistent identity: range1126806
display id: range1126806
version: 1
direction: direction/forward
top level: BBa_I11030
start: 1195
end: 1200
orientation: inline

BBa_K108024
type: ComponentDefinition
persistent identity: BBa K108024
display id: BBa_K108024
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RuvC
creator: Qi Liu
created: 2008-10-22T11:00:00Z
modified: 2015-05-08T01:09:04Z
discontinued: false
dominant: false
experience: experience/None
group u list: _222_
m user id: 0
owner id: 2671
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: component of RuvABC resolvasome, endonuclease
mutable notes: PCR using primers with biobrick prefix and surfix, and then cloned into pSB1AC3.
mutable provenance: E.coli K12 DH10B genome
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: RuvC
sequence: https://synbiohub.org/public/igem/BBa_K108024_sequence/1

RuvC
type: SequenceAnnotation
persistent identity: annotation1986979
display id: annotation1986979
version: 1
top level: BBa_K108024
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K108024/annotation1986979/range1986979/1

https://synbiohub.org/public/igem/BBa_K108024/annotation1986979/range1986979/1
type: Range
persistent identity: range1986979
display id: range1986979
version: 1
direction: direction/forward
top level: BBa_K108024
start: 1
end: 519
orientation: inline

Hin
type: ComponentDefinition
persistent identity: BBa J31000
display id: BBa_J31000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: DNA-invertase Hin from Salmonella typhimurium
creator: Erin Zwack, Sabriya Rosemond
created: 2006-06-04T11:00:00Z
modified: 2015-08-31T04:08:45Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _61_
m user id: 0
owner id: 918
owning group id: 61
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Generates the Salmonella typhimurium Hin protein. This protein is used to invert DNA that is flanked by Hix siteds.
mutable notes: none really
mutable provenance: Salmonella typhimurium
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Hin sequence
sequence annotation: Rev primer J31001
sequence annotation: Mutation to delete the SpeI site
sequence annotation: Fwd primer J31001
sequence annotation: Former SpeI site
sequence: https://synbiohub.org/public/igem/BBa_J31000_sequence/1

Hin sequence
type: SequenceAnnotation
persistent identity: annotation1884987
display id: annotation1884987
version: 1
top level: Hin
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J31000/annotation1884987/range1884987/1

https://synbiohub.org/public/igem/BBa_J31000/annotation1884987/range1884987/1
type: Range
persistent identity: range1884987
display id: range1884987
version: 1
direction: direction/forward
top level: Hin
start: 1
end: 573
orientation: inline

Rev primer J31001
type: SequenceAnnotation
persistent identity: annotation1910569
display id: annotation1910569
version: 1
top level: Hin
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_J31000/annotation1910569/range1910569/1

https://synbiohub.org/public/igem/BBa_J31000/annotation1910569/range1910569/1
type: Range
persistent identity: range1910569
display id: range1910569
version: 1
direction: direction/reverse
top level: Hin
start: 551
end: 570
orientation: inline

Mutation to delete the SpeI site
type: SequenceAnnotation
persistent identity: annotation1880460
display id: annotation1880460
version: 1
top level: Hin
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_J31000/annotation1880460/range1880460/1

https://synbiohub.org/public/igem/BBa_J31000/annotation1880460/range1880460/1
type: Range
persistent identity: range1880460
display id: range1880460
version: 1
direction: direction/forward
top level: Hin
start: 72
end: 72
orientation: inline

Fwd primer J31001
type: SequenceAnnotation
persistent identity: annotation1910568
display id: annotation1910568
version: 1
top level: Hin
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_J31000/annotation1910568/range1910568/1

https://synbiohub.org/public/igem/BBa_J31000/annotation1910568/range1910568/1
type: Range
persistent identity: range1910568
display id: range1910568
version: 1
direction: direction/forward
top level: Hin
start: 1
end: 3
orientation: inline

Former SpeI site
type: SequenceAnnotation
persistent identity: annotation1880459
display id: annotation1880459
version: 1
top level: Hin
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_J31000/annotation1880459/range1880459/1

https://synbiohub.org/public/igem/BBa_J31000/annotation1880459/range1880459/1
type: Range
persistent identity: range1880459
display id: range1880459
version: 1
direction: direction/forward
top level: Hin
start: 70
end: 75
orientation: inline

BBa_K112223
type: ComponentDefinition
persistent identity: BBa K112223
display id: BBa_K112223
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: {a~ihfB!} The ihf beta gene ready to have rbs attached and stop codon, BBb format
creator: Molly Allen
created: 2008-10-20T11:00:00Z
modified: 2015-05-08T01:09:15Z
discontinued: false
dominant: false
experience: experience/None
group u list: _224_
m user id: 0
owner id: 2048
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at: [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
mutable notes: ?
mutable provenance: MG1655
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K112223_sequence/1

BBa_K112219
type: ComponentDefinition
persistent identity: BBa K112219
display id: BBa_K112219
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: {ihfB!} The ihf beta gene with start and stop codons, BBb format
creator: Molly Allen
created: 2008-10-20T11:00:00Z
modified: 2015-05-08T01:09:15Z
discontinued: false
dominant: false
experience: experience/None
group u list: _224_
m user id: 0
owner id: 2048
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at: [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
mutable notes: ?
mutable provenance: MG1655
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K112219_sequence/1

BBa_K112220
type: ComponentDefinition
persistent identity: BBa K112220
display id: BBa_K112220
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: {< ihfB!} The ihf beta gene without a start codon, BBb format
creator: Molly Allen
created: 2008-10-20T11:00:00Z
modified: 2015-05-08T01:09:15Z
discontinued: false
dominant: false
experience: experience/None
group u list: _224_
m user id: 0
owner id: 2048
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at: [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
mutable notes: ?
mutable provenance: MG1655
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence: https://synbiohub.org/public/igem/BBa_K112220_sequence/1