//function/odor
type: Collection
persistent identity: function odor
display id: function_odor
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
owned by: james
owned by: myers
member: JAMT
member: BAT2
member: BBa_K1919002
member: SWOG
member: BBa_K118003
member: BBa_K1919003
member: ATF1
member: BAMT
member: pchBA
member: Banana
member: rbs+COMT
member: BSMT1
member: BBa_J45219
member: THI3
member: rbs+LIMS1
member: Wintergree
member: SG
member: BOBS
member: ATF1 gener
member: WOG
member: YYC912
member: SAMT
member: BOG
member: EWOBS
member: BBa_J45099
member: BBa_J45180
member: BBa_J45396
member: BBa_J45220
member: COMT
member: SBOBS
member: VanBP
member: BBa_K1919001
member: BBa_J45270
member: BSMT2
member: WOBS

pchBA
type: ComponentDefinition
persistent identity: BBa J45017
display id: BBa_J45017
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: isochorismate pyruvate-lyase and isochorismate synthase (pchBA); converts chorismate to salicylate
creator: MIT IGEM 2006
created: 2006-08-20T11:00:00Z
modified: 2015-08-31T04:08:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _84_
m user id: 0
owner id: 1147
owning group id: 84
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: pchBA codes for proteins used to generate salicylic acid. It doesn't make a fusion protein, even though the coding regions overlap; the RBS for pchA is located at the end of the coding region of pchB.
mutable notes: n/a
mutable provenance: pchBA is found in pseudomonas. The plasmid was kindly sent to the MIT iGEM team by Dr. Cornelia Riemmann from the Universite de Lausanne.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: pchB start codon
sequence annotation: PchB
sequence annotation: PchA
sequence annotation: pchA double TAA stop codon
sequence annotation: g -> a to eliminate PstI site
sequence annotation: pchB stop codon
sequence annotation: pchA start codon
sequence: https://synbiohub.org/public/igem/BBa_J45017_sequence/1

pchB start codon
type: SequenceAnnotation
persistent identity: annotation1897455
display id: annotation1897455
version: 1
top level: pchBA
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_J45017/annotation1897455/range1897455/1

https://synbiohub.org/public/igem/BBa_J45017/annotation1897455/range1897455/1
type: Range
persistent identity: range1897455
display id: range1897455
version: 1
direction: direction/forward
top level: pchBA
start: 1
end: 3
orientation: inline

PchB
type: SequenceAnnotation
persistent identity: annotation1961296
display id: annotation1961296
version: 1
top level: pchBA
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J45017/annotation1961296/range1961296/1

https://synbiohub.org/public/igem/BBa_J45017/annotation1961296/range1961296/1
type: Range
persistent identity: range1961296
display id: range1961296
version: 1
direction: direction/forward
top level: pchBA
start: 1
end: 306
orientation: inline

PchA
type: SequenceAnnotation
persistent identity: annotation1961297
display id: annotation1961297
version: 1
top level: pchBA
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J45017/annotation1961297/range1961297/1

https://synbiohub.org/public/igem/BBa_J45017/annotation1961297/range1961297/1
type: Range
persistent identity: range1961297
display id: range1961297
version: 1
direction: direction/forward
top level: pchBA
start: 303
end: 1736
orientation: inline

pchA double TAA stop codon
type: SequenceAnnotation
persistent identity: annotation1897457
display id: annotation1897457
version: 1
top level: pchBA
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_J45017/annotation1897457/range1897457/1

https://synbiohub.org/public/igem/BBa_J45017/annotation1897457/range1897457/1
type: Range
persistent identity: range1897457
display id: range1897457
version: 1
direction: direction/forward
top level: pchBA
start: 1731
end: 1736
orientation: inline

g -> a to eliminate PstI site
type: SequenceAnnotation
persistent identity: annotation1897453
display id: annotation1897453
version: 1
top level: pchBA
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_J45017/annotation1897453/range1897453/1

https://synbiohub.org/public/igem/BBa_J45017/annotation1897453/range1897453/1
type: Range
persistent identity: range1897453
display id: range1897453
version: 1
direction: direction/forward
top level: pchBA
start: 440
end: 440
orientation: inline

pchB stop codon
type: SequenceAnnotation
persistent identity: annotation1897456
display id: annotation1897456
version: 1
top level: pchBA
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_J45017/annotation1897456/range1897456/1

https://synbiohub.org/public/igem/BBa_J45017/annotation1897456/range1897456/1
type: Range
persistent identity: range1897456
display id: range1897456
version: 1
direction: direction/forward
top level: pchBA
start: 304
end: 306
orientation: inline

pchA start codon
type: SequenceAnnotation
persistent identity: annotation1897454
display id: annotation1897454
version: 1
top level: pchBA
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_J45017/annotation1897454/range1897454/1

https://synbiohub.org/public/igem/BBa_J45017/annotation1897454/range1897454/1
type: Range
persistent identity: range1897454
display id: range1897454
version: 1
direction: direction/forward
top level: pchBA
start: 303
end: 305
orientation: inline

BBa_J45219
type: ComponentDefinition
persistent identity: BBa J45219
display id: BBa_J45219
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Banana odor enzyme (ATF1) generator
creator: Andr?? Green II
created: 2006-10-27T11:00:00Z
modified: 2015-08-31T04:08:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _84_
m user id: 0
owner id: 977
owning group id: 84
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This part is an intermediate to the protein generator BBa_45220. This part is almost identical to BBa_J45199 except that it uses B0032 as its RBS (instead of B0030).
mutable notes: coming
mutable provenance: coming
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0032
component: BBa_B0012
component: BBa_J45014
component: BBa_B0010
sequence annotation: BBa_B0032
sequence annotation: BBa_B0010
sequence annotation: BBa_J45014
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_J45219_sequence/1

BBa_B0032
type: Component
persistent identity: component1908023
display id: component1908023
version: 1
top level: BBa_J45219
access: public
definition: https://synbiohub.org/public/igem/BBa_B0032/1

BBa_B0012
type: Component
persistent identity: component1908032
display id: component1908032
version: 1
top level: BBa_J45219
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_J45014
type: Component
persistent identity: component1908029
display id: component1908029
version: 1
top level: BBa_J45219
access: public
definition: ATF1

BBa_B0010
type: Component
persistent identity: component1908030
display id: component1908030
version: 1
top level: BBa_J45219
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation1908023
display id: annotation1908023
version: 1
top level: BBa_J45219
location: https://synbiohub.org/public/igem/BBa_J45219/annotation1908023/range1908023/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_J45219/annotation1908023/range1908023/1
type: Range
persistent identity: range1908023
display id: range1908023
version: 1
direction: direction/forward
top level: BBa_J45219
start: 1
end: 13
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1908030
display id: annotation1908030
version: 1
top level: BBa_J45219
location: https://synbiohub.org/public/igem/BBa_J45219/annotation1908030/range1908030/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_J45219/annotation1908030/range1908030/1
type: Range
persistent identity: range1908030
display id: range1908030
version: 1
direction: direction/forward
top level: BBa_J45219
start: 1609
end: 1688
orientation: inline

BBa_J45014
type: SequenceAnnotation
persistent identity: annotation1908029
display id: annotation1908029
version: 1
top level: BBa_J45219
location: https://synbiohub.org/public/igem/BBa_J45219/annotation1908029/range1908029/1
component: BBa_J45014

https://synbiohub.org/public/igem/BBa_J45219/annotation1908029/range1908029/1
type: Range
persistent identity: range1908029
display id: range1908029
version: 1
direction: direction/reverse
top level: BBa_J45219
start: 20
end: 1600
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1908032
display id: annotation1908032
version: 1
top level: BBa_J45219
location: https://synbiohub.org/public/igem/BBa_J45219/annotation1908032/range1908032/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_J45219/annotation1908032/range1908032/1
type: Range
persistent identity: range1908032
display id: range1908032
version: 1
direction: direction/forward
top level: BBa_J45219
start: 1697
end: 1737
orientation: inline

BBa_J45220
type: ComponentDefinition
persistent identity: BBa J45220
display id: BBa_J45220
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Banana odor generator
creator: Andr?? Green II
created: 2006-10-19T11:00:00Z
modified: 2015-08-31T04:08:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _84_
m user id: 0
owner id: 977
owning group id: 84
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Protein generator converting PoPS to the protein ATF1. ATFI encodes the enzyme alcohol acetyltransferase I (AATase I), which produces acetate esters from acetyl CoA and alcohols. This part is similar to BBa_J45200, except that it uses the B0032 RBS instead of B0030 (which is in BBa_J45200).
mutable notes: This part is the ATF sequence BBa_45J006 with a single point mutation at base pair 1148 (T -> C). Despite the mutation, the enzyme is still functional (kinetic studies have not been done to determine realitive functionality).
mutable provenance: None.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_J45014
component: BBa_B0032
component: BBa_B0010
component: BBa_B0012
component: BBa_R0011
sequence annotation: BBa_B0012
sequence annotation: BBa_B0010
sequence annotation: BBa_J45014
sequence annotation: BBa_B0032
sequence annotation: BBa_R0011
sequence: https://synbiohub.org/public/igem/BBa_J45220_sequence/1

BBa_J45014
type: Component
persistent identity: component1903718
display id: component1903718
version: 1
top level: BBa_J45220
access: public
definition: ATF1

BBa_B0032
type: Component
persistent identity: component1903712
display id: component1903712
version: 1
top level: BBa_J45220
access: public
definition: https://synbiohub.org/public/igem/BBa_B0032/1

BBa_B0010
type: Component
persistent identity: component1903719
display id: component1903719
version: 1
top level: BBa_J45220
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
type: Component
persistent identity: component1903721
display id: component1903721
version: 1
top level: BBa_J45220
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_R0011
type: Component
persistent identity: component1903706
display id: component1903706
version: 1
top level: BBa_J45220
access: public
definition: https://synbiohub.org/public/igem/BBa_R0011/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1903721
display id: annotation1903721
version: 1
top level: BBa_J45220
location: https://synbiohub.org/public/igem/BBa_J45220/annotation1903721/range1903721/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_J45220/annotation1903721/range1903721/1
type: Range
persistent identity: range1903721
display id: range1903721
version: 1
direction: direction/forward
top level: BBa_J45220
start: 1760
end: 1800
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1903719
display id: annotation1903719
version: 1
top level: BBa_J45220
location: https://synbiohub.org/public/igem/BBa_J45220/annotation1903719/range1903719/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_J45220/annotation1903719/range1903719/1
type: Range
persistent identity: range1903719
display id: range1903719
version: 1
direction: direction/forward
top level: BBa_J45220
start: 1672
end: 1751
orientation: inline

BBa_J45014
type: SequenceAnnotation
persistent identity: annotation1903718
display id: annotation1903718
version: 1
top level: BBa_J45220
location: https://synbiohub.org/public/igem/BBa_J45220/annotation1903718/range1903718/1
component: BBa_J45014

https://synbiohub.org/public/igem/BBa_J45220/annotation1903718/range1903718/1
type: Range
persistent identity: range1903718
display id: range1903718
version: 1
direction: direction/reverse
top level: BBa_J45220
start: 83
end: 1663
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation1903712
display id: annotation1903712
version: 1
top level: BBa_J45220
location: https://synbiohub.org/public/igem/BBa_J45220/annotation1903712/range1903712/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_J45220/annotation1903712/range1903712/1
type: Range
persistent identity: range1903712
display id: range1903712
version: 1
direction: direction/forward
top level: BBa_J45220
start: 64
end: 76
orientation: inline

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation1903706
display id: annotation1903706
version: 1
top level: BBa_J45220
location: https://synbiohub.org/public/igem/BBa_J45220/annotation1903706/range1903706/1
component: BBa_R0011

https://synbiohub.org/public/igem/BBa_J45220/annotation1903706/range1903706/1
type: Range
persistent identity: range1903706
display id: range1903706
version: 1
direction: direction/forward
top level: BBa_J45220
start: 1
end: 54
orientation: inline

BSMT1
type: ComponentDefinition
persistent identity: BBa J45004
display id: BBa_J45004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: SAM:benzoic acid/salicylic acid carboxyl methyltransferase I; converts salicylic acid to methyl sali
creator: Boyuan Zhu
created: 2006-06-06T11:00:00Z
modified: 2015-08-31T04:08:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _84_
m user id: 0
owner id: 974
owning group id: 84
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: Codes for BSMT enzyme to convert Benzoic acid/salicylic acid to methyl benzoate and methyl salicylate. This part is phBSMT1 from Petunia x hybrida.
mutable notes: Transport issues are being considered.
mutable provenance: Species: Petunia x hybrida from Genbank; (also available: A. thaliana and N. suaveolens)
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: start codon
sequence annotation: BMST1
sequence annotation: stop codon
sequence: https://synbiohub.org/public/igem/BBa_J45004_sequence/1

start codon
type: SequenceAnnotation
persistent identity: annotation1880519
display id: annotation1880519
version: 1
top level: BSMT1
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_J45004/annotation1880519/range1880519/1

https://synbiohub.org/public/igem/BBa_J45004/annotation1880519/range1880519/1
type: Range
persistent identity: range1880519
display id: range1880519
version: 1
direction: direction/forward
top level: BSMT1
start: 1
end: 3
orientation: inline

BMST1
type: SequenceAnnotation
persistent identity: annotation1880517
display id: annotation1880517
version: 1
top level: BSMT1
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J45004/annotation1880517/range1880517/1

https://synbiohub.org/public/igem/BBa_J45004/annotation1880517/range1880517/1
type: Range
persistent identity: range1880517
display id: range1880517
version: 1
direction: direction/forward
top level: BSMT1
start: 1
end: 1074
orientation: inline

stop codon
type: SequenceAnnotation
persistent identity: annotation1880520
display id: annotation1880520
version: 1
top level: BSMT1
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_J45004/annotation1880520/range1880520/1

https://synbiohub.org/public/igem/BBa_J45004/annotation1880520/range1880520/1
type: Range
persistent identity: range1880520
display id: range1880520
version: 1
direction: direction/forward
top level: BSMT1
start: 1072
end: 1074
orientation: inline

WOG
type: ComponentDefinition
persistent identity: BBa J45100
display id: BBa_J45100
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Wintergreen odor enzyme (BSMT1) generator
creator: Boyuan Zhu
created: 2006-08-08T11:00:00Z
modified: 2015-08-31T04:08:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _84_
m user id: 0
owner id: 974
owning group id: 84
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This is the wintergreen generating device hooked up to a constitutive promoter.
mutable notes: See J45099
mutable provenance: This is made from a biobrick promoter and our biobrick wintergreen generating device.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0012
component: BBa_R0040
component: BBa_B0030
component: BBa_J45004
component: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_J45004
sequence annotation: BBa_B0010
sequence annotation: BBa_B0030
sequence annotation: BBa_R0040
sequence: https://synbiohub.org/public/igem/BBa_J45100_sequence/1

BBa_B0012
type: Component
persistent identity: component1894594
display id: component1894594
version: 1
top level: WOG
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_R0040
type: Component
persistent identity: component1894581
display id: component1894581
version: 1
top level: WOG
access: public
definition: https://synbiohub.org/public/igem/BBa_R0040/1

BBa_B0030
type: Component
persistent identity: component1894586
display id: component1894586
version: 1
top level: WOG
access: public
definition: https://synbiohub.org/public/igem/BBa_B0030/1

BBa_J45004
type: Component
persistent identity: component1894591
display id: component1894591
version: 1
top level: WOG
access: public
definition: BSMT1

BBa_B0010
type: Component
persistent identity: component1894592
display id: component1894592
version: 1
top level: WOG
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1894594
display id: annotation1894594
version: 1
top level: WOG
location: https://synbiohub.org/public/igem/BBa_J45100/annotation1894594/range1894594/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_J45100/annotation1894594/range1894594/1
type: Range
persistent identity: range1894594
display id: range1894594
version: 1
direction: direction/forward
top level: WOG
start: 1254
end: 1294
orientation: inline

BBa_J45004
type: SequenceAnnotation
persistent identity: annotation1894591
display id: annotation1894591
version: 1
top level: WOG
location: https://synbiohub.org/public/igem/BBa_J45100/annotation1894591/range1894591/1
component: BBa_J45004

https://synbiohub.org/public/igem/BBa_J45100/annotation1894591/range1894591/1
type: Range
persistent identity: range1894591
display id: range1894591
version: 1
direction: direction/reverse
top level: WOG
start: 84
end: 1157
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1894592
display id: annotation1894592
version: 1
top level: WOG
location: https://synbiohub.org/public/igem/BBa_J45100/annotation1894592/range1894592/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_J45100/annotation1894592/range1894592/1
type: Range
persistent identity: range1894592
display id: range1894592
version: 1
direction: direction/forward
top level: WOG
start: 1166
end: 1245
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation1894586
display id: annotation1894586
version: 1
top level: WOG
location: https://synbiohub.org/public/igem/BBa_J45100/annotation1894586/range1894586/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_J45100/annotation1894586/range1894586/1
type: Range
persistent identity: range1894586
display id: range1894586
version: 1
direction: direction/forward
top level: WOG
start: 63
end: 77
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1894581
display id: annotation1894581
version: 1
top level: WOG
location: https://synbiohub.org/public/igem/BBa_J45100/annotation1894581/range1894581/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_J45100/annotation1894581/range1894581/1
type: Range
persistent identity: range1894581
display id: range1894581
version: 1
direction: direction/forward
top level: WOG
start: 1
end: 54
orientation: inline

BBa_J45099
type: ComponentDefinition
persistent identity: BBa J45099
display id: BBa_J45099
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Wintergreen odor enzyme (BSMT1) generator
creator: Boyuan Zhu
created: 2006-08-08T11:00:00Z
modified: 2015-08-31T04:08:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _84_
m user id: 0
owner id: 974
owning group id: 84
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This composite part include a ribosome binding site, the BSMT coding region, and a terminator. This part is attached to different terminators.
mutable notes: We made and used a B0030 backbone.
mutable provenance: We used the earlier composite biobrick part of the BSMT+Terminator and attached a RBS from the registry.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_J45004
component: BBa_B0030
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_J45004
sequence annotation: BBa_B0030
sequence: https://synbiohub.org/public/igem/BBa_J45099_sequence/1

BBa_J45004
type: Component
persistent identity: component1894570
display id: component1894570
version: 1
top level: BBa_J45099
access: public
definition: BSMT1

BBa_B0030
type: Component
persistent identity: component1894565
display id: component1894565
version: 1
top level: BBa_J45099
access: public
definition: https://synbiohub.org/public/igem/BBa_B0030/1

BBa_B0012
type: Component
persistent identity: component1894573
display id: component1894573
version: 1
top level: BBa_J45099
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0010
type: Component
persistent identity: component1894571
display id: component1894571
version: 1
top level: BBa_J45099
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1894571
display id: annotation1894571
version: 1
top level: BBa_J45099
location: https://synbiohub.org/public/igem/BBa_J45099/annotation1894571/range1894571/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_J45099/annotation1894571/range1894571/1
type: Range
persistent identity: range1894571
display id: range1894571
version: 1
direction: direction/forward
top level: BBa_J45099
start: 1104
end: 1183
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1894573
display id: annotation1894573
version: 1
top level: BBa_J45099
location: https://synbiohub.org/public/igem/BBa_J45099/annotation1894573/range1894573/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_J45099/annotation1894573/range1894573/1
type: Range
persistent identity: range1894573
display id: range1894573
version: 1
direction: direction/forward
top level: BBa_J45099
start: 1192
end: 1232
orientation: inline

BBa_J45004
type: SequenceAnnotation
persistent identity: annotation1894570
display id: annotation1894570
version: 1
top level: BBa_J45099
location: https://synbiohub.org/public/igem/BBa_J45099/annotation1894570/range1894570/1
component: BBa_J45004

https://synbiohub.org/public/igem/BBa_J45099/annotation1894570/range1894570/1
type: Range
persistent identity: range1894570
display id: range1894570
version: 1
direction: direction/reverse
top level: BBa_J45099
start: 22
end: 1095
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation1894565
display id: annotation1894565
version: 1
top level: BBa_J45099
location: https://synbiohub.org/public/igem/BBa_J45099/annotation1894565/range1894565/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_J45099/annotation1894565/range1894565/1
type: Range
persistent identity: range1894565
display id: range1894565
version: 1
direction: direction/forward
top level: BBa_J45099
start: 1
end: 15
orientation: inline

BBa_K1919003
type: ComponentDefinition
persistent identity: BBa K1919003
display id: BBa_K1919003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: T7 promotor (under the control of lacI) and downstream CecropinXJ
creator: Shiji Zhao
created: 2016-09-06T11:00:00Z
modified: 2016-10-14T10:12:45Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2386_
m user id: 26103
owner id: 26103
owning group id: 9
sample status: No part sequence
status: status/Planning
bookmark: false
mutable description: Antimicrobial peptide CecropinXJ belongs to AMP family Cecropin, a group of small basic polypeptides mainly found in the hemolymph of insects, consist of 31-39 amino acid residues and have a broad spectrum, high heat stability and potent bacteriostatic activity.
mutable notes: The coding sequence was optimized for E.coli expression using JCat http://www.jcat.de/. The single stop codon was replaced by double TAA stop codon.
mutable provenance: The original sequence of CecropinXJ comes from GenBank NM_001043995.1. The sample used for experiments was artificially synthesized by Tsingke.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
sequence annotation: TrxA
sequence annotation: lacI
sequence annotation: T7 promotor
sequence annotation: lac operator
sequence annotation: S tag
sequence annotation: T7 terminator
sequence annotation: enterokinase site
sequence annotation: lacI promotor
sequence annotation: cecropinXJ
sequence annotation: rrnB T1 terminator
sequence annotation: 6xHis Tag
sequence annotation: Thrombin site
sequence: https://synbiohub.org/public/igem/BBa_K1919003_sequence/1

TrxA
type: SequenceAnnotation
persistent identity: annotation2506817
display id: annotation2506817
version: 1
top level: BBa_K1919003
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1919003/annotation2506817/range2506817/1

https://synbiohub.org/public/igem/BBa_K1919003/annotation2506817/range2506817/1
type: Range
persistent identity: range2506817
display id: range2506817
version: 1
direction: direction/forward
top level: BBa_K1919003
start: 1562
end: 1888
orientation: inline

lacI
type: SequenceAnnotation
persistent identity: annotation2506825
display id: annotation2506825
version: 1
top level: BBa_K1919003
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1919003/annotation2506825/range2506825/1

https://synbiohub.org/public/igem/BBa_K1919003/annotation2506825/range2506825/1
type: Range
persistent identity: range2506825
display id: range2506825
version: 1
direction: direction/reverse
top level: BBa_K1919003
start: 1
end: 1083
orientation: inline

T7 promotor
type: SequenceAnnotation
persistent identity: annotation2506815
display id: annotation2506815
version: 1
top level: BBa_K1919003
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1919003/annotation2506815/range2506815/1

https://synbiohub.org/public/igem/BBa_K1919003/annotation2506815/range2506815/1
type: Range
persistent identity: range2506815
display id: range2506815
version: 1
direction: direction/forward
top level: BBa_K1919003
start: 1474
end: 1492
orientation: inline

lac operator
type: SequenceAnnotation
persistent identity: annotation2506816
display id: annotation2506816
version: 1
top level: BBa_K1919003
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K1919003/annotation2506816/range2506816/1

https://synbiohub.org/public/igem/BBa_K1919003/annotation2506816/range2506816/1
type: Range
persistent identity: range2506816
display id: range2506816
version: 1
direction: direction/forward
top level: BBa_K1919003
start: 1493
end: 1517
orientation: inline

S tag
type: SequenceAnnotation
persistent identity: annotation2506820
display id: annotation2506820
version: 1
top level: BBa_K1919003
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1919003/annotation2506820/range2506820/1

https://synbiohub.org/public/igem/BBa_K1919003/annotation2506820/range2506820/1
type: Range
persistent identity: range2506820
display id: range2506820
version: 1
direction: direction/forward
top level: BBa_K1919003
start: 1961
end: 2005
orientation: inline

T7 terminator
type: SequenceAnnotation
persistent identity: annotation2506823
display id: annotation2506823
version: 1
top level: BBa_K1919003
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1919003/annotation2506823/range2506823/1

https://synbiohub.org/public/igem/BBa_K1919003/annotation2506823/range2506823/1
type: Range
persistent identity: range2506823
display id: range2506823
version: 1
direction: direction/forward
top level: BBa_K1919003
start: 2342
end: 2389
orientation: inline

enterokinase site
type: SequenceAnnotation
persistent identity: annotation2506821
display id: annotation2506821
version: 1
top level: BBa_K1919003
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1919003/annotation2506821/range2506821/1

https://synbiohub.org/public/igem/BBa_K1919003/annotation2506821/range2506821/1
type: Range
persistent identity: range2506821
display id: range2506821
version: 1
direction: direction/forward
top level: BBa_K1919003
start: 2021
end: 2035
orientation: inline

lacI promotor
type: SequenceAnnotation
persistent identity: annotation2506814
display id: annotation2506814
version: 1
top level: BBa_K1919003
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1919003/annotation2506814/range2506814/1

https://synbiohub.org/public/igem/BBa_K1919003/annotation2506814/range2506814/1
type: Range
persistent identity: range2506814
display id: range2506814
version: 1
direction: direction/reverse
top level: BBa_K1919003
start: 1084
end: 1161
orientation: inline

cecropinXJ
type: SequenceAnnotation
persistent identity: annotation2506822
display id: annotation2506822
version: 1
top level: BBa_K1919003
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1919003/annotation2506822/range2506822/1

https://synbiohub.org/public/igem/BBa_K1919003/annotation2506822/range2506822/1
type: Range
persistent identity: range2506822
display id: range2506822
version: 1
direction: direction/forward
top level: BBa_K1919003
start: 2057
end: 2245
orientation: inline

rrnB T1 terminator
type: SequenceAnnotation
persistent identity: annotation2506824
display id: annotation2506824
version: 1
top level: BBa_K1919003
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1919003/annotation2506824/range2506824/1

https://synbiohub.org/public/igem/BBa_K1919003/annotation2506824/range2506824/1
type: Range
persistent identity: range2506824
display id: range2506824
version: 1
direction: direction/forward
top level: BBa_K1919003
start: 2399
end: 2444
orientation: inline

6xHis Tag
type: SequenceAnnotation
persistent identity: annotation2506818
display id: annotation2506818
version: 1
top level: BBa_K1919003
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1919003/annotation2506818/range2506818/1

https://synbiohub.org/public/igem/BBa_K1919003/annotation2506818/range2506818/1
type: Range
persistent identity: range2506818
display id: range2506818
version: 1
direction: direction/forward
top level: BBa_K1919003
start: 1910
end: 1927
orientation: inline

Thrombin site
type: SequenceAnnotation
persistent identity: annotation2506819
display id: annotation2506819
version: 1
top level: BBa_K1919003
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1919003/annotation2506819/range2506819/1

https://synbiohub.org/public/igem/BBa_K1919003/annotation2506819/range2506819/1
type: Range
persistent identity: range2506819
display id: range2506819
version: 1
direction: direction/forward
top level: BBa_K1919003
start: 1937
end: 1954
orientation: inline

SWOG
type: ComponentDefinition
persistent identity: BBa J45170
display id: BBa_J45170
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Stationary-phase-dependent wintergreen odor generator
creator: MIT IGEM 2006
created: 2006-08-09T11:00:00Z
modified: 2015-08-31T04:08:49Z
discontinued: false
dominant: false
experience: experience/None
group u list: _84_
m user id: 0
owner id: 1147
owning group id: 84
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This is the wintergreen generating device hooked up to a stationary phase promoter with RBS B0032.
mutable notes: Biobricks of osmY promoter and intermediate 45119.
mutable provenance: Still in development as SPP is not confirmed to work.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_J45993
component: BBa_B0032
component: BBa_B0012
component: BBa_J45004
component: BBa_B0010
sequence annotation: BBa_J45004
sequence annotation: BBa_B0010
sequence annotation: BBa_B0032
sequence annotation: BBa_B0012
sequence annotation: BBa_J45993
sequence: https://synbiohub.org/public/igem/BBa_J45170_sequence/1

BBa_J45993
type: Component
persistent identity: component1894801
display id: component1894801
version: 1
top level: SWOG
access: public
definition: https://synbiohub.org/public/igem/BBa_J45993/1

BBa_B0032
type: Component
persistent identity: component1894803
display id: component1894803
version: 1
top level: SWOG
access: public
definition: https://synbiohub.org/public/igem/BBa_B0032/1

BBa_B0012
type: Component
persistent identity: component1894811
display id: component1894811
version: 1
top level: SWOG
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_J45004
type: Component
persistent identity: component1894808
display id: component1894808
version: 1
top level: SWOG
access: public
definition: BSMT1

BBa_B0010
type: Component
persistent identity: component1894809
display id: component1894809
version: 1
top level: SWOG
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_J45004
type: SequenceAnnotation
persistent identity: annotation1894808
display id: annotation1894808
version: 1
top level: SWOG
location: https://synbiohub.org/public/igem/BBa_J45170/annotation1894808/range1894808/1
component: BBa_J45004

https://synbiohub.org/public/igem/BBa_J45170/annotation1894808/range1894808/1
type: Range
persistent identity: range1894808
display id: range1894808
version: 1
direction: direction/reverse
top level: SWOG
start: 85
end: 1158
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1894809
display id: annotation1894809
version: 1
top level: SWOG
location: https://synbiohub.org/public/igem/BBa_J45170/annotation1894809/range1894809/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_J45170/annotation1894809/range1894809/1
type: Range
persistent identity: range1894809
display id: range1894809
version: 1
direction: direction/forward
top level: SWOG
start: 1167
end: 1246
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation1894803
display id: annotation1894803
version: 1
top level: SWOG
location: https://synbiohub.org/public/igem/BBa_J45170/annotation1894803/range1894803/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_J45170/annotation1894803/range1894803/1
type: Range
persistent identity: range1894803
display id: range1894803
version: 1
direction: direction/forward
top level: SWOG
start: 66
end: 78
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1894811
display id: annotation1894811
version: 1
top level: SWOG
location: https://synbiohub.org/public/igem/BBa_J45170/annotation1894811/range1894811/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_J45170/annotation1894811/range1894811/1
type: Range
persistent identity: range1894811
display id: range1894811
version: 1
direction: direction/forward
top level: SWOG
start: 1255
end: 1295
orientation: inline

BBa_J45993
type: SequenceAnnotation
persistent identity: annotation1894801
display id: annotation1894801
version: 1
top level: SWOG
location: https://synbiohub.org/public/igem/BBa_J45170/annotation1894801/range1894801/1
component: BBa_J45993

https://synbiohub.org/public/igem/BBa_J45170/annotation1894801/range1894801/1
type: Range
persistent identity: range1894801
display id: range1894801
version: 1
direction: direction/reverse
top level: SWOG
start: 1
end: 57
orientation: inline

BBa_J45180
type: ComponentDefinition
persistent identity: BBa J45180
display id: BBa_J45180
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Exponential phase dependent wintergreen odor generator
creator: Andr?? Green II
created: 2006-10-30T12:00:00Z
modified: 2015-08-31T04:08:49Z
discontinued: false
dominant: false
experience: experience/Fails
group u list: _84_
m user id: 0
owner id: 977
owning group id: 84
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is a protein generator that outputs BSMT (benzoic acid/salicylic acid methyl transferase) under control of the osmY stationary phase promoter and Q04400 inverter. Ideally, when in stationary phase, BSMT output is "low" and vice versa.
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0032
component: BBa_J45993
component: BBa_Q04401
component: BBa_B0012
component: BBa_B0010
component: BBa_J45004
sequence annotation: BBa_B0012
sequence annotation: BBa_J45004
sequence annotation: BBa_B0010
sequence annotation: BBa_J45993
sequence annotation: BBa_Q04401
sequence annotation: BBa_B0032
sequence: https://synbiohub.org/public/igem/BBa_J45180_sequence/1

BBa_B0032
type: Component
persistent identity: component1909155
display id: component1909155
version: 1
top level: BBa_J45180
access: public
definition: https://synbiohub.org/public/igem/BBa_B0032/1

BBa_J45993
type: Component
persistent identity: component1909152
display id: component1909152
version: 1
top level: BBa_J45180
access: public
definition: https://synbiohub.org/public/igem/BBa_J45993/1

BBa_Q04401
type: Component
persistent identity: component1909153
display id: component1909153
version: 1
top level: BBa_J45180
access: public
definition: https://synbiohub.org/public/igem/BBa_Q04401/1

BBa_B0012
type: Component
persistent identity: component1909163
display id: component1909163
version: 1
top level: BBa_J45180
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0010
type: Component
persistent identity: component1909161
display id: component1909161
version: 1
top level: BBa_J45180
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_J45004
type: Component
persistent identity: component1909160
display id: component1909160
version: 1
top level: BBa_J45180
access: public
definition: BSMT1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1909163
display id: annotation1909163
version: 1
top level: BBa_J45180
location: https://synbiohub.org/public/igem/BBa_J45180/annotation1909163/range1909163/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_J45180/annotation1909163/range1909163/1
type: Range
persistent identity: range1909163
display id: range1909163
version: 1
direction: direction/forward
top level: BBa_J45180
start: 2165
end: 2205
orientation: inline

BBa_J45004
type: SequenceAnnotation
persistent identity: annotation1909160
display id: annotation1909160
version: 1
top level: BBa_J45180
location: https://synbiohub.org/public/igem/BBa_J45180/annotation1909160/range1909160/1
component: BBa_J45004

https://synbiohub.org/public/igem/BBa_J45180/annotation1909160/range1909160/1
type: Range
persistent identity: range1909160
display id: range1909160
version: 1
direction: direction/reverse
top level: BBa_J45180
start: 995
end: 2068
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1909161
display id: annotation1909161
version: 1
top level: BBa_J45180
location: https://synbiohub.org/public/igem/BBa_J45180/annotation1909161/range1909161/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_J45180/annotation1909161/range1909161/1
type: Range
persistent identity: range1909161
display id: range1909161
version: 1
direction: direction/forward
top level: BBa_J45180
start: 2077
end: 2156
orientation: inline

BBa_J45993
type: SequenceAnnotation
persistent identity: annotation1909152
display id: annotation1909152
version: 1
top level: BBa_J45180
location: https://synbiohub.org/public/igem/BBa_J45180/annotation1909152/range1909152/1
component: BBa_J45993

https://synbiohub.org/public/igem/BBa_J45180/annotation1909152/range1909152/1
type: Range
persistent identity: range1909152
display id: range1909152
version: 1
direction: direction/reverse
top level: BBa_J45180
start: 1
end: 57
orientation: inline

BBa_Q04401
type: SequenceAnnotation
persistent identity: annotation1909153
display id: annotation1909153
version: 1
top level: BBa_J45180
location: https://synbiohub.org/public/igem/BBa_J45180/annotation1909153/range1909153/1
component: BBa_Q04401

https://synbiohub.org/public/igem/BBa_J45180/annotation1909153/range1909153/1
type: Range
persistent identity: range1909153
display id: range1909153
version: 1
direction: direction/reverse
top level: BBa_J45180
start: 66
end: 967
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation1909155
display id: annotation1909155
version: 1
top level: BBa_J45180
location: https://synbiohub.org/public/igem/BBa_J45180/annotation1909155/range1909155/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_J45180/annotation1909155/range1909155/1
type: Range
persistent identity: range1909155
display id: range1909155
version: 1
direction: direction/forward
top level: BBa_J45180
start: 976
end: 988
orientation: inline

EWOBS
type: ComponentDefinition
persistent identity: BBa J45500
display id: BBa_J45500
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Exponential phase wintergreen odor biosynthetic system
creator: MIT iGEM 2006
created: 2006-06-19T11:00:00Z
modified: 2015-08-31T04:08:49Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_84_
m user id: 0
owner id: 2
owning group id: 84
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: prkA from Synechococcus PCC 7942
mutable notes: ...
mutable provenance: NCBI
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
component: BBa_J45181
component: BBa_J45320
sequence annotation: BBa_J45320
sequence annotation: BBa_J45181
sequence: https://synbiohub.org/public/igem/BBa_J45500_sequence/1

BBa_J45181
type: Component
persistent identity: component2291026
display id: component2291026
version: 1
top level: EWOBS
access: public
definition: Wintergree

BBa_J45320
type: Component
persistent identity: component2291006
display id: component2291006
version: 1
top level: EWOBS
access: public
definition: SG

BBa_J45320
type: SequenceAnnotation
persistent identity: annotation2291006
display id: annotation2291006
version: 1
top level: EWOBS
location: https://synbiohub.org/public/igem/BBa_J45500/annotation2291006/range2291006/1
component: BBa_J45320

https://synbiohub.org/public/igem/BBa_J45500/annotation2291006/range2291006/1
type: Range
persistent identity: range2291006
display id: range2291006
version: 1
direction: direction/reverse
top level: EWOBS
start: 1
end: 1955
orientation: inline

BBa_J45181
type: SequenceAnnotation
persistent identity: annotation2291026
display id: annotation2291026
version: 1
top level: EWOBS
location: https://synbiohub.org/public/igem/BBa_J45500/annotation2291026/range2291026/1
component: BBa_J45181

https://synbiohub.org/public/igem/BBa_J45500/annotation2291026/range2291026/1
type: Range
persistent identity: range2291026
display id: range2291026
version: 1
direction: direction/reverse
top level: EWOBS
start: 1964
end: 4310
orientation: inline

BAT2
type: ComponentDefinition
persistent identity: BBa J45008
display id: BBa_J45008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: branched-chain amino acid transaminase (BAT2); converts leucine to alpha-ketoisocaproate
creator: Veena Venkatachalam
created: 2006-06-06T11:00:00Z
modified: 2015-08-31T04:08:49Z
discontinued: false
dominant: false
experience: experience/Fails
group u list: _84_
m user id: 0
owner id: 642
owning group id: 84
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: fd;lsfas
mutable notes: dakfdas;
mutable provenance: dkfkas
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: BAT2
sequence annotation: double TAA stop codon
sequence annotation: start codon
sequence annotation: a->g to eliminate SpeI
sequence: https://synbiohub.org/public/igem/BBa_J45008_sequence/1

BAT2
type: SequenceAnnotation
persistent identity: annotation1961299
display id: annotation1961299
version: 1
top level: BAT2
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J45008/annotation1961299/range1961299/1

https://synbiohub.org/public/igem/BBa_J45008/annotation1961299/range1961299/1
type: Range
persistent identity: range1961299
display id: range1961299
version: 1
direction: direction/forward
top level: BAT2
start: 1
end: 1134
orientation: inline

double TAA stop codon
type: SequenceAnnotation
persistent identity: annotation1883625
display id: annotation1883625
version: 1
top level: BAT2
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_J45008/annotation1883625/range1883625/1

https://synbiohub.org/public/igem/BBa_J45008/annotation1883625/range1883625/1
type: Range
persistent identity: range1883625
display id: range1883625
version: 1
direction: direction/forward
top level: BAT2
start: 1129
end: 1134
orientation: inline

start codon
type: SequenceAnnotation
persistent identity: annotation1883624
display id: annotation1883624
version: 1
top level: BAT2
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_J45008/annotation1883624/range1883624/1

https://synbiohub.org/public/igem/BBa_J45008/annotation1883624/range1883624/1
type: Range
persistent identity: range1883624
display id: range1883624
version: 1
direction: direction/forward
top level: BAT2
start: 1
end: 3
orientation: inline

a->g to eliminate SpeI
type: SequenceAnnotation
persistent identity: annotation1894553
display id: annotation1894553
version: 1
top level: BAT2
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_J45008/annotation1894553/range1894553/1

https://synbiohub.org/public/igem/BBa_J45008/annotation1894553/range1894553/1
type: Range
persistent identity: range1894553
display id: range1894553
version: 1
direction: direction/forward
top level: BAT2
start: 771
end: 771
orientation: inline

SBOBS
type: ComponentDefinition
persistent identity: BBa J45600
display id: BBa_J45600
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Stationary phase banana odor biosynthetic system
creator: MIT iGEM 2006
created: 2006-10-30T12:00:00Z
modified: 2015-08-31T04:08:50Z
discontinued: false
dominant: false
experience: experience/None
group u list: _84_
m user id: 0
owner id: 2
owning group id: 84
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This system designed by the MIT 2006 iGEM team generates mint smell by biosynthesizing salicylic acid from chassis-produced metabolites (chorismate) and then converts salicylic acid into methyl salicylate (wintergreen scent).
mutable notes: We recommend use of strain <bbpart>BBa_J45999</bbpart> as it does not produce indole. Indole is responsible for the foul smell of wt e.coli, and masks the wintergreen scent produced by J45600.
mutable provenance: This system is composed of devices <bbpart>J45120</bbpart> and <bbpart>J45320</bbpart>.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
component: BBa_B0010
component: BBa_R0011
component: BBa_B0030
component: BBa_J45014
component: BBa_B0012
component: BBa_J45008
component: BBa_J45992
component: BBa_J45009
sequence annotation: BBa_B0010
sequence annotation: BBa_J45014
sequence annotation: BBa_J45008
sequence annotation: BBa_B0012
sequence annotation: BBa_J45992
sequence annotation: BBa_B0030
sequence annotation: BBa_J45009
sequence annotation: BBa_R0011
sequence: https://synbiohub.org/public/igem/BBa_J45600_sequence/1

BBa_B0010
type: Component
persistent identity: component1961071
display id: component1961071
version: 1
top level: SBOBS
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_R0011
type: Component
persistent identity: component1961077
display id: component1961077
version: 1
top level: SBOBS
access: public
definition: https://synbiohub.org/public/igem/BBa_R0011/1

BBa_B0030
type: Component
persistent identity: component1961064
display id: component1961064
version: 1
top level: SBOBS
access: public
definition: https://synbiohub.org/public/igem/BBa_B0030/1

BBa_J45014
type: Component
persistent identity: component1961070
display id: component1961070
version: 1
top level: SBOBS
access: public
definition: ATF1

BBa_B0012
type: Component
persistent identity: component1961073
display id: component1961073
version: 1
top level: SBOBS
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0030
type: Component
persistent identity: component1961090
display id: component1961090
version: 1
top level: SBOBS
access: public
definition: https://synbiohub.org/public/igem/BBa_B0030/1

BBa_J45008
type: Component
persistent identity: component1961088
display id: component1961088
version: 1
top level: SBOBS
access: public
definition: BAT2

BBa_J45992
type: Component
persistent identity: component1961062
display id: component1961062
version: 1
top level: SBOBS
access: public
definition: https://synbiohub.org/public/igem/BBa_J45992/1

BBa_B0030
type: Component
persistent identity: component1961083
display id: component1961083
version: 1
top level: SBOBS
access: public
definition: https://synbiohub.org/public/igem/BBa_B0030/1

BBa_J45009
type: Component
persistent identity: component1961097
display id: component1961097
version: 1
top level: SBOBS
access: public
definition: THI3

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1961071
display id: annotation1961071
version: 1
top level: SBOBS
location: https://synbiohub.org/public/igem/BBa_J45600/annotation1961071/range1961071/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_J45600/annotation1961071/range1961071/1
type: Range
persistent identity: range1961071
display id: range1961071
version: 1
direction: direction/forward
top level: SBOBS
start: 1818
end: 1897
orientation: inline

BBa_J45014
type: SequenceAnnotation
persistent identity: annotation1961070
display id: annotation1961070
version: 1
top level: SBOBS
location: https://synbiohub.org/public/igem/BBa_J45600/annotation1961070/range1961070/1
component: BBa_J45014

https://synbiohub.org/public/igem/BBa_J45600/annotation1961070/range1961070/1
type: Range
persistent identity: range1961070
display id: range1961070
version: 1
direction: direction/reverse
top level: SBOBS
start: 229
end: 1809
orientation: inline

BBa_J45008
type: SequenceAnnotation
persistent identity: annotation1961088
display id: annotation1961088
version: 1
top level: SBOBS
location: https://synbiohub.org/public/igem/BBa_J45600/annotation1961088/range1961088/1
component: BBa_J45008

https://synbiohub.org/public/igem/BBa_J45600/annotation1961088/range1961088/1
type: Range
persistent identity: range1961088
display id: range1961088
version: 1
direction: direction/reverse
top level: SBOBS
start: 2039
end: 3172
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1961073
display id: annotation1961073
version: 1
top level: SBOBS
location: https://synbiohub.org/public/igem/BBa_J45600/annotation1961073/range1961073/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_J45600/annotation1961073/range1961073/1
type: Range
persistent identity: range1961073
display id: range1961073
version: 1
direction: direction/forward
top level: SBOBS
start: 1906
end: 1946
orientation: inline

BBa_J45992
type: SequenceAnnotation
persistent identity: annotation1961062
display id: annotation1961062
version: 1
top level: SBOBS
location: https://synbiohub.org/public/igem/BBa_J45600/annotation1961062/range1961062/1
component: BBa_J45992

https://synbiohub.org/public/igem/BBa_J45600/annotation1961062/range1961062/1
type: Range
persistent identity: range1961062
display id: range1961062
version: 1
direction: direction/reverse
top level: SBOBS
start: 1
end: 199
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation1961064
display id: annotation1961064
version: 1
top level: SBOBS
location: https://synbiohub.org/public/igem/BBa_J45600/annotation1961064/range1961064/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_J45600/annotation1961064/range1961064/1
type: Range
persistent identity: range1961064
display id: range1961064
version: 1
direction: direction/forward
top level: SBOBS
start: 208
end: 222
orientation: inline

BBa_J45009
type: SequenceAnnotation
persistent identity: annotation1961097
display id: annotation1961097
version: 1
top level: SBOBS
location: https://synbiohub.org/public/igem/BBa_J45600/annotation1961097/range1961097/1
component: BBa_J45009

https://synbiohub.org/public/igem/BBa_J45600/annotation1961097/range1961097/1
type: Range
persistent identity: range1961097
display id: range1961097
version: 1
direction: direction/reverse
top level: SBOBS
start: 3202
end: 5031
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation1961083
display id: annotation1961083
version: 1
top level: SBOBS
location: https://synbiohub.org/public/igem/BBa_J45600/annotation1961083/range1961083/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_J45600/annotation1961083/range1961083/1
type: Range
persistent identity: range1961083
display id: range1961083
version: 1
direction: direction/forward
top level: SBOBS
start: 2018
end: 2032
orientation: inline

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation1961077
display id: annotation1961077
version: 1
top level: SBOBS
location: https://synbiohub.org/public/igem/BBa_J45600/annotation1961077/range1961077/1
component: BBa_R0011

https://synbiohub.org/public/igem/BBa_J45600/annotation1961077/range1961077/1
type: Range
persistent identity: range1961077
display id: range1961077
version: 1
direction: direction/forward
top level: SBOBS
start: 1955
end: 2008
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation1961090
display id: annotation1961090
version: 1
top level: SBOBS
location: https://synbiohub.org/public/igem/BBa_J45600/annotation1961090/range1961090/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_J45600/annotation1961090/range1961090/1
type: Range
persistent identity: range1961090
display id: range1961090
version: 1
direction: direction/forward
top level: SBOBS
start: 3181
end: 3195
orientation: inline

BOBS
type: ComponentDefinition
persistent identity: BBa J45900
display id: BBa_J45900
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Banana odor biosynthetic system
creator: Stephen Payne
created: 2008-02-04T12:00:00Z
modified: 2015-08-31T04:08:50Z
discontinued: false
dominant: false
experience: experience/Fails
group u list: _84_
m user id: 0
owner id: 642
owning group id: 84
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This part does not work.
mutable notes: This part has EcoRI and SpeI sites in it. Use caution when conducting standard assembly with the part.
mutable provenance: This part was made via standard assembly of J45200 and J45400.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
component: BBa_B0030
component: BBa_J45008
component: BBa_B0010
component: BBa_J45014
component: BBa_B0012
component: BBa_J45009
component: BBa_R0040
component: BBa_R0011
sequence annotation: BBa_B0030
sequence annotation: BBa_J45014
sequence annotation: BBa_R0040
sequence annotation: BBa_J45009
sequence annotation: BBa_B0010
sequence annotation: BBa_R0011
sequence annotation: BBa_J45008
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_J45900_sequence/1

BBa_B0030
type: Component
persistent identity: component1960848
display id: component1960848
version: 1
top level: BOBS
access: public
definition: https://synbiohub.org/public/igem/BBa_B0030/1

BBa_J45008
type: Component
persistent identity: component1960853
display id: component1960853
version: 1
top level: BOBS
access: public
definition: BAT2

BBa_B0030
type: Component
persistent identity: component1960869
display id: component1960869
version: 1
top level: BOBS
access: public
definition: https://synbiohub.org/public/igem/BBa_B0030/1

BBa_B0010
type: Component
persistent identity: component1960876
display id: component1960876
version: 1
top level: BOBS
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_J45014
type: Component
persistent identity: component1960875
display id: component1960875
version: 1
top level: BOBS
access: public
definition: ATF1

BBa_B0012
type: Component
persistent identity: component1960878
display id: component1960878
version: 1
top level: BOBS
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0030
type: Component
persistent identity: component1960855
display id: component1960855
version: 1
top level: BOBS
access: public
definition: https://synbiohub.org/public/igem/BBa_B0030/1

BBa_J45009
type: Component
persistent identity: component1960862
display id: component1960862
version: 1
top level: BOBS
access: public
definition: THI3

BBa_R0040
type: Component
persistent identity: component1960864
display id: component1960864
version: 1
top level: BOBS
access: public
definition: https://synbiohub.org/public/igem/BBa_R0040/1

BBa_R0011
type: Component
persistent identity: component1960842
display id: component1960842
version: 1
top level: BOBS
access: public
definition: https://synbiohub.org/public/igem/BBa_R0011/1

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation1960848
display id: annotation1960848
version: 1
top level: BOBS
location: https://synbiohub.org/public/igem/BBa_J45900/annotation1960848/range1960848/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_J45900/annotation1960848/range1960848/1
type: Range
persistent identity: range1960848
display id: range1960848
version: 1
direction: direction/forward
top level: BOBS
start: 64
end: 78
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation1960869
display id: annotation1960869
version: 1
top level: BOBS
location: https://synbiohub.org/public/igem/BBa_J45900/annotation1960869/range1960869/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_J45900/annotation1960869/range1960869/1
type: Range
persistent identity: range1960869
display id: range1960869
version: 1
direction: direction/forward
top level: BOBS
start: 3148
end: 3162
orientation: inline

BBa_J45014
type: SequenceAnnotation
persistent identity: annotation1960875
display id: annotation1960875
version: 1
top level: BOBS
location: https://synbiohub.org/public/igem/BBa_J45900/annotation1960875/range1960875/1
component: BBa_J45014

https://synbiohub.org/public/igem/BBa_J45900/annotation1960875/range1960875/1
type: Range
persistent identity: range1960875
display id: range1960875
version: 1
direction: direction/reverse
top level: BOBS
start: 3169
end: 4749
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1960864
display id: annotation1960864
version: 1
top level: BOBS
location: https://synbiohub.org/public/igem/BBa_J45900/annotation1960864/range1960864/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_J45900/annotation1960864/range1960864/1
type: Range
persistent identity: range1960864
display id: range1960864
version: 1
direction: direction/forward
top level: BOBS
start: 3086
end: 3139
orientation: inline

BBa_J45009
type: SequenceAnnotation
persistent identity: annotation1960862
display id: annotation1960862
version: 1
top level: BOBS
location: https://synbiohub.org/public/igem/BBa_J45900/annotation1960862/range1960862/1
component: BBa_J45009

https://synbiohub.org/public/igem/BBa_J45900/annotation1960862/range1960862/1
type: Range
persistent identity: range1960862
display id: range1960862
version: 1
direction: direction/reverse
top level: BOBS
start: 1248
end: 3077
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1960876
display id: annotation1960876
version: 1
top level: BOBS
location: https://synbiohub.org/public/igem/BBa_J45900/annotation1960876/range1960876/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_J45900/annotation1960876/range1960876/1
type: Range
persistent identity: range1960876
display id: range1960876
version: 1
direction: direction/forward
top level: BOBS
start: 4758
end: 4837
orientation: inline

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation1960842
display id: annotation1960842
version: 1
top level: BOBS
location: https://synbiohub.org/public/igem/BBa_J45900/annotation1960842/range1960842/1
component: BBa_R0011

https://synbiohub.org/public/igem/BBa_J45900/annotation1960842/range1960842/1
type: Range
persistent identity: range1960842
display id: range1960842
version: 1
direction: direction/forward
top level: BOBS
start: 1
end: 54
orientation: inline

BBa_J45008
type: SequenceAnnotation
persistent identity: annotation1960853
display id: annotation1960853
version: 1
top level: BOBS
location: https://synbiohub.org/public/igem/BBa_J45900/annotation1960853/range1960853/1
component: BBa_J45008

https://synbiohub.org/public/igem/BBa_J45900/annotation1960853/range1960853/1
type: Range
persistent identity: range1960853
display id: range1960853
version: 1
direction: direction/reverse
top level: BOBS
start: 85
end: 1218
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1960878
display id: annotation1960878
version: 1
top level: BOBS
location: https://synbiohub.org/public/igem/BBa_J45900/annotation1960878/range1960878/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_J45900/annotation1960878/range1960878/1
type: Range
persistent identity: range1960878
display id: range1960878
version: 1
direction: direction/forward
top level: BOBS
start: 4846
end: 4886
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation1960855
display id: annotation1960855
version: 1
top level: BOBS
location: https://synbiohub.org/public/igem/BBa_J45900/annotation1960855/range1960855/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_J45900/annotation1960855/range1960855/1
type: Range
persistent identity: range1960855
display id: range1960855
version: 1
direction: direction/forward
top level: BOBS
start: 1227
end: 1241
orientation: inline

SAMT
type: ComponentDefinition
persistent identity: BBa J45001
display id: BBa_J45001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: SAM:salicylic acid carboxyl methyltransferase; converts salicylic acid to methyl salicylate (winter
creator: Kate Broadbent
created: 2006-06-06T11:00:00Z
modified: 2015-08-31T04:08:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _84_
m user id: 0
owner id: 976
owning group id: 84
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: From snapdragon (A. Majus)
mutable notes: lala
mutable provenance: Dutareva
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: SAMT
sequence annotation: start codon
sequence annotation: double TAA stop codon
sequence: https://synbiohub.org/public/igem/BBa_J45001_sequence/1

SAMT
type: SequenceAnnotation
persistent identity: annotation1880595
display id: annotation1880595
version: 1
top level: SAMT
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J45001/annotation1880595/range1880595/1

https://synbiohub.org/public/igem/BBa_J45001/annotation1880595/range1880595/1
type: Range
persistent identity: range1880595
display id: range1880595
version: 1
direction: direction/forward
top level: SAMT
start: 1
end: 1151
orientation: inline

start codon
type: SequenceAnnotation
persistent identity: annotation1880596
display id: annotation1880596
version: 1
top level: SAMT
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_J45001/annotation1880596/range1880596/1

https://synbiohub.org/public/igem/BBa_J45001/annotation1880596/range1880596/1
type: Range
persistent identity: range1880596
display id: range1880596
version: 1
direction: direction/forward
top level: SAMT
start: 1
end: 3
orientation: inline

double TAA stop codon
type: SequenceAnnotation
persistent identity: annotation1880597
display id: annotation1880597
version: 1
top level: SAMT
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_J45001/annotation1880597/range1880597/1

https://synbiohub.org/public/igem/BBa_J45001/annotation1880597/range1880597/1
type: Range
persistent identity: range1880597
display id: range1880597
version: 1
direction: direction/forward
top level: SAMT
start: 1150
end: 1155
orientation: inline

SAMT
type: ComponentDefinition
persistent identity: BBa J45005
display id: BBa_J45005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: SAM:salicylic acid carboxyl methyltransferase; converts salicylic acid to methyl salicylate (winterg
creator: Kate Broadbent
created: 2006-06-06T11:00:00Z
modified: 2015-08-31T04:08:49Z
discontinued: false
dominant: false
experience: experience/None
group u list: _84_
m user id: 0
owner id: 976
owning group id: 84
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: This is the SAMT enzyme from C. Breweri
mutable notes: we need an MTA or to sequence the coding region for this enzyme
mutable provenance: Genbank: AF133053, Eran Pichersky
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: SAMT
sequence annotation: double TAA stop codon
sequence annotation: start codon
sequence: https://synbiohub.org/public/igem/BBa_J45005_sequence/1

SAMT
type: SequenceAnnotation
persistent identity: annotation1880525
display id: annotation1880525
version: 1
top level: SAMT
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J45005/annotation1880525/range1880525/1

https://synbiohub.org/public/igem/BBa_J45005/annotation1880525/range1880525/1
type: Range
persistent identity: range1880525
display id: range1880525
version: 1
direction: direction/forward
top level: SAMT
start: 1
end: 1083
orientation: inline

double TAA stop codon
type: SequenceAnnotation
persistent identity: annotation1880522
display id: annotation1880522
version: 1
top level: SAMT
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_J45005/annotation1880522/range1880522/1

https://synbiohub.org/public/igem/BBa_J45005/annotation1880522/range1880522/1
type: Range
persistent identity: range1880522
display id: range1880522
version: 1
direction: direction/forward
top level: SAMT
start: 1078
end: 1083
orientation: inline

start codon
type: SequenceAnnotation
persistent identity: annotation1880479
display id: annotation1880479
version: 1
top level: SAMT
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_J45005/annotation1880479/range1880479/1

https://synbiohub.org/public/igem/BBa_J45005/annotation1880479/range1880479/1
type: Range
persistent identity: range1880479
display id: range1880479
version: 1
direction: direction/forward
top level: SAMT
start: 1
end: 3
orientation: inline

BBa_K1919001
type: ComponentDefinition
persistent identity: BBa K1919001
display id: BBa_K1919001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Constitutive promotor J23105 and downstream CecropinXJ
creator: Shiji Zhao
created: 2016-09-04T11:00:00Z
modified: 2016-10-14T08:28:15Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2386_
m user id: 26103
owner id: 26103
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Biobrick BBa_K1919001 is a composite part, consisting of constitutive promotor J23105, RBS, TrxA tag, 6xHis tag, thrombin site, S tag, enterkinase site and the coding sequence of antimicrobial peptide CecropinXJ.
mutable notes: Two steps were needed in order to obtain BBa_K1919001. Step 1, BBa_K1919000 was inserted into pET32a(+). The primers used for PCR are as following: F: 5'-CCGGGATCCATGAACTTCGCTAAAATCCTGTCTT-3' (BamH I site was added) R: 5'-CCGCTCGAGTTATTATTTACCGATAGCTTTA-3' (Xho I site was added) BamH I/Xho I Double enzymatic cutting was used for both pET32a(+) and BBa_K1919000. Step 2, RBS and 5 downstream tags as well as original CecropinXJ was inserted at the downstream of promotor J23105 with the same procedure as step 1. The primers used for PCR are as following: F: 5'-GCTCTAGAGCGAAGGAGATATACATATGAGCG-3' (Xba I site was added) R: 5'-TGCACTGCAGTGCAGACTAGTCGTTATTGCTCAGCGGTGG-3'(Pst I site was added) Xba I/Pst I double enzymatic cutting was used for PCR product and Spe I/Pst I double enzymatic cutting was used for BBa_J23105. After enzymatic linkage, the construction of BBa_K1919001 was finished. Then this part was added to pSB1C3 backbone.
mutable provenance: The sequence of constitutive promotor J23105 comes from biobrick BBa_J23105. RBS and 5 downstream tags come from commercial plasmid pET32a(+). The sequence of CecropinXJ comes from biobrick BBa_K1919000.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
sequence annotation: ATG start codon
sequence annotation: 6xHis Tag
sequence annotation: BBa_K1919000
sequence annotation: CecropinXJ coding sequence
sequence annotation: thrombin site
sequence annotation: enterokinase site
sequence annotation: double TAA stop codon
sequence annotation: BBa_J23105
sequence annotation: TrxA
sequence annotation: S-Tag
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_K1919001_sequence/1

ATG start codon
type: SequenceAnnotation
persistent identity: annotation2482469
display id: annotation2482469
version: 1
top level: BBa_K1919001
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1919001/annotation2482469/range2482469/1

https://synbiohub.org/public/igem/BBa_K1919001/annotation2482469/range2482469/1
type: Range
persistent identity: range2482469
display id: range2482469
version: 1
direction: direction/forward
top level: BBa_K1919001
start: 60
end: 62
orientation: inline

6xHis Tag
type: SequenceAnnotation
persistent identity: annotation2482475
display id: annotation2482475
version: 1
top level: BBa_K1919001
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1919001/annotation2482475/range2482475/1

https://synbiohub.org/public/igem/BBa_K1919001/annotation2482475/range2482475/1
type: Range
persistent identity: range2482475
display id: range2482475
version: 1
direction: direction/forward
top level: BBa_K1919001
start: 408
end: 425
orientation: inline

BBa_K1919000
type: SequenceAnnotation
persistent identity: annotation2482472
display id: annotation2482472
version: 1
top level: BBa_K1919001
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1919001/annotation2482472/range2482472/1

https://synbiohub.org/public/igem/BBa_K1919001/annotation2482472/range2482472/1
type: Range
persistent identity: range2482472
display id: range2482472
version: 1
direction: direction/forward
top level: BBa_K1919001
start: 555
end: 749
orientation: inline

CecropinXJ coding sequence
type: SequenceAnnotation
persistent identity: annotation2482470
display id: annotation2482470
version: 1
top level: BBa_K1919001
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1919001/annotation2482470/range2482470/1

https://synbiohub.org/public/igem/BBa_K1919001/annotation2482470/range2482470/1
type: Range
persistent identity: range2482470
display id: range2482470
version: 1
direction: direction/forward
top level: BBa_K1919001
start: 555
end: 749
orientation: inline

thrombin site
type: SequenceAnnotation
persistent identity: annotation2482476
display id: annotation2482476
version: 1
top level: BBa_K1919001
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1919001/annotation2482476/range2482476/1

https://synbiohub.org/public/igem/BBa_K1919001/annotation2482476/range2482476/1
type: Range
persistent identity: range2482476
display id: range2482476
version: 1
direction: direction/forward
top level: BBa_K1919001
start: 435
end: 452
orientation: inline

enterokinase site
type: SequenceAnnotation
persistent identity: annotation2482478
display id: annotation2482478
version: 1
top level: BBa_K1919001
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1919001/annotation2482478/range2482478/1

https://synbiohub.org/public/igem/BBa_K1919001/annotation2482478/range2482478/1
type: Range
persistent identity: range2482478
display id: range2482478
version: 1
direction: direction/forward
top level: BBa_K1919001
start: 519
end: 533
orientation: inline

double TAA stop codon
type: SequenceAnnotation
persistent identity: annotation2482471
display id: annotation2482471
version: 1
top level: BBa_K1919001
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1919001/annotation2482471/range2482471/1

https://synbiohub.org/public/igem/BBa_K1919001/annotation2482471/range2482471/1
type: Range
persistent identity: range2482471
display id: range2482471
version: 1
direction: direction/forward
top level: BBa_K1919001
start: 744
end: 749
orientation: inline

BBa_J23105
type: SequenceAnnotation
persistent identity: annotation2482468
display id: annotation2482468
version: 1
top level: BBa_K1919001
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1919001/annotation2482468/range2482468/1

https://synbiohub.org/public/igem/BBa_K1919001/annotation2482468/range2482468/1
type: Range
persistent identity: range2482468
display id: range2482468
version: 1
direction: direction/forward
top level: BBa_K1919001
start: 1
end: 35
orientation: inline

TrxA
type: SequenceAnnotation
persistent identity: annotation2482474
display id: annotation2482474
version: 1
top level: BBa_K1919001
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1919001/annotation2482474/range2482474/1

https://synbiohub.org/public/igem/BBa_K1919001/annotation2482474/range2482474/1
type: Range
persistent identity: range2482474
display id: range2482474
version: 1
direction: direction/forward
top level: BBa_K1919001
start: 60
end: 386
orientation: inline

S-Tag
type: SequenceAnnotation
persistent identity: annotation2482477
display id: annotation2482477
version: 1
top level: BBa_K1919001
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1919001/annotation2482477/range2482477/1

https://synbiohub.org/public/igem/BBa_K1919001/annotation2482477/range2482477/1
type: Range
persistent identity: range2482477
display id: range2482477
version: 1
direction: direction/forward
top level: BBa_K1919001
start: 459
end: 503
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2482473
display id: annotation2482473
version: 1
top level: BBa_K1919001
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1919001/annotation2482473/range2482473/1

https://synbiohub.org/public/igem/BBa_K1919001/annotation2482473/range2482473/1
type: Range
persistent identity: range2482473
display id: range2482473
version: 1
direction: direction/forward
top level: BBa_K1919001
start: 46
end: 51
orientation: inline

WOBS
type: ComponentDefinition
persistent identity: BBa J45700
display id: BBa_J45700
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Wintergreen odor biosynthetic system
creator: MIT iGEM 2006
created: 2006-10-30T12:00:00Z
modified: 2015-08-31T04:08:50Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _84_
m user id: 0
owner id: 2
owning group id: 84
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: This system designed by the MIT 2006 iGEM team and generates mint smell by biosynthesizing salicylic acid from chassis-produced metabolites (chorismate) and then converts salicylic acid into methyl salicylate (wintergreen scent).
mutable notes: We recommend use of strain <bbpart>BBa_J45999</bbpart> as it does not produce indole. Indole is responsible for the foul smell of wt e.coli, and masks the wintergreen scent produced by J45600.
mutable provenance: This system is composed of devices <bbpart>J45120</bbpart> and <bbpart>J45320</bbpart>.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000804
role: partType/Project
component: BBa_B0032
component: BBa_J45004
component: BBa_R0011
component: BBa_B0012
component: BBa_R0040
component: BBa_J45017
component: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_J45017
sequence annotation: BBa_R0040
sequence annotation: BBa_B0010
sequence annotation: BBa_B0032
sequence annotation: BBa_J45004
sequence annotation: BBa_R0011
sequence: https://synbiohub.org/public/igem/BBa_J45700_sequence/1

BBa_B0032
type: Component
persistent identity: component1960989
display id: component1960989
version: 1
top level: WOBS
access: public
definition: https://synbiohub.org/public/igem/BBa_B0032/1

BBa_J45004
type: Component
persistent identity: component1960976
display id: component1960976
version: 1
top level: WOBS
access: public
definition: BSMT1

BBa_R0011
type: Component
persistent identity: component1960983
display id: component1960983
version: 1
top level: WOBS
access: public
definition: https://synbiohub.org/public/igem/BBa_R0011/1

BBa_B0012
type: Component
persistent identity: component1960979
display id: component1960979
version: 1
top level: WOBS
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0032
type: Component
persistent identity: component1960971
display id: component1960971
version: 1
top level: WOBS
access: public
definition: https://synbiohub.org/public/igem/BBa_B0032/1

BBa_R0040
type: Component
persistent identity: component1960966
display id: component1960966
version: 1
top level: WOBS
access: public
definition: https://synbiohub.org/public/igem/BBa_R0040/1

BBa_B0012
type: Component
persistent identity: component1960999
display id: component1960999
version: 1
top level: WOBS
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_J45017
type: Component
persistent identity: component1960996
display id: component1960996
version: 1
top level: WOBS
access: public
definition: pchBA

BBa_B0010
type: Component
persistent identity: component1960997
display id: component1960997
version: 1
top level: WOBS
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0010
type: Component
persistent identity: component1960977
display id: component1960977
version: 1
top level: WOBS
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1960999
display id: annotation1960999
version: 1
top level: WOBS
location: https://synbiohub.org/public/igem/BBa_J45700/annotation1960999/range1960999/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_J45700/annotation1960999/range1960999/1
type: Range
persistent identity: range1960999
display id: range1960999
version: 1
direction: direction/forward
top level: WOBS
start: 3215
end: 3255
orientation: inline

BBa_J45017
type: SequenceAnnotation
persistent identity: annotation1960996
display id: annotation1960996
version: 1
top level: WOBS
location: https://synbiohub.org/public/igem/BBa_J45700/annotation1960996/range1960996/1
component: BBa_J45017

https://synbiohub.org/public/igem/BBa_J45700/annotation1960996/range1960996/1
type: Range
persistent identity: range1960996
display id: range1960996
version: 1
direction: direction/reverse
top level: WOBS
start: 1383
end: 3118
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1960966
display id: annotation1960966
version: 1
top level: WOBS
location: https://synbiohub.org/public/igem/BBa_J45700/annotation1960966/range1960966/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_J45700/annotation1960966/range1960966/1
type: Range
persistent identity: range1960966
display id: range1960966
version: 1
direction: direction/forward
top level: WOBS
start: 1
end: 54
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1960979
display id: annotation1960979
version: 1
top level: WOBS
location: https://synbiohub.org/public/igem/BBa_J45700/annotation1960979/range1960979/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_J45700/annotation1960979/range1960979/1
type: Range
persistent identity: range1960979
display id: range1960979
version: 1
direction: direction/forward
top level: WOBS
start: 1252
end: 1292
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1960977
display id: annotation1960977
version: 1
top level: WOBS
location: https://synbiohub.org/public/igem/BBa_J45700/annotation1960977/range1960977/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_J45700/annotation1960977/range1960977/1
type: Range
persistent identity: range1960977
display id: range1960977
version: 1
direction: direction/forward
top level: WOBS
start: 1164
end: 1243
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation1960989
display id: annotation1960989
version: 1
top level: WOBS
location: https://synbiohub.org/public/igem/BBa_J45700/annotation1960989/range1960989/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_J45700/annotation1960989/range1960989/1
type: Range
persistent identity: range1960989
display id: range1960989
version: 1
direction: direction/forward
top level: WOBS
start: 1364
end: 1376
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation1960971
display id: annotation1960971
version: 1
top level: WOBS
location: https://synbiohub.org/public/igem/BBa_J45700/annotation1960971/range1960971/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_J45700/annotation1960971/range1960971/1
type: Range
persistent identity: range1960971
display id: range1960971
version: 1
direction: direction/forward
top level: WOBS
start: 63
end: 75
orientation: inline

BBa_J45004
type: SequenceAnnotation
persistent identity: annotation1960976
display id: annotation1960976
version: 1
top level: WOBS
location: https://synbiohub.org/public/igem/BBa_J45700/annotation1960976/range1960976/1
component: BBa_J45004

https://synbiohub.org/public/igem/BBa_J45700/annotation1960976/range1960976/1
type: Range
persistent identity: range1960976
display id: range1960976
version: 1
direction: direction/reverse
top level: WOBS
start: 82
end: 1155
orientation: inline

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation1960983
display id: annotation1960983
version: 1
top level: WOBS
location: https://synbiohub.org/public/igem/BBa_J45700/annotation1960983/range1960983/1
component: BBa_R0011

https://synbiohub.org/public/igem/BBa_J45700/annotation1960983/range1960983/1
type: Range
persistent identity: range1960983
display id: range1960983
version: 1
direction: direction/forward
top level: WOBS
start: 1301
end: 1354
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1960997
display id: annotation1960997
version: 1
top level: WOBS
location: https://synbiohub.org/public/igem/BBa_J45700/annotation1960997/range1960997/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_J45700/annotation1960997/range1960997/1
type: Range
persistent identity: range1960997
display id: range1960997
version: 1
direction: direction/forward
top level: WOBS
start: 3127
end: 3206
orientation: inline

ATF1
type: ComponentDefinition
persistent identity: BBa J45014
display id: BBa_J45014
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: alcohol acetyltransferase I; converts isoamyl alcohol to isoamyl acetate (banana odor)
creator: Andr?? Green II
created: 2006-07-30T11:00:00Z
modified: 2015-08-31T04:08:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _84_
m user id: 0
owner id: 977
owning group id: 84
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: This part is the ATF sequence (BBa_45J006) with a single point mutation at base pair 1176 (T -> C).
mutable notes: It has a single point mutation at base pair 1176 (T -> C).
mutable provenance: This part comes from BBa_45J006.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: ATF1
sequence annotation: a->g to eliminate EcoRI site
sequence annotation: t -> c
sequence annotation: double TAA stop codon
sequence: https://synbiohub.org/public/igem/BBa_J45014_sequence/1

ATF1
type: SequenceAnnotation
persistent identity: annotation1961300
display id: annotation1961300
version: 1
top level: ATF1
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J45014/annotation1961300/range1961300/1

https://synbiohub.org/public/igem/BBa_J45014/annotation1961300/range1961300/1
type: Range
persistent identity: range1961300
display id: range1961300
version: 1
direction: direction/forward
top level: ATF1
start: 1
end: 1581
orientation: inline

a->g to eliminate EcoRI site
type: SequenceAnnotation
persistent identity: annotation1961305
display id: annotation1961305
version: 1
top level: ATF1
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_J45014/annotation1961305/range1961305/1

https://synbiohub.org/public/igem/BBa_J45014/annotation1961305/range1961305/1
type: Range
persistent identity: range1961305
display id: range1961305
version: 1
direction: direction/forward
top level: ATF1
start: 414
end: 414
orientation: inline

t -> c
type: SequenceAnnotation
persistent identity: annotation1961301
display id: annotation1961301
version: 1
top level: ATF1
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_J45014/annotation1961301/range1961301/1

https://synbiohub.org/public/igem/BBa_J45014/annotation1961301/range1961301/1
type: Range
persistent identity: range1961301
display id: range1961301
version: 1
direction: direction/forward
top level: ATF1
start: 1148
end: 1148
orientation: inline

double TAA stop codon
type: SequenceAnnotation
persistent identity: annotation1961302
display id: annotation1961302
version: 1
top level: ATF1
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_J45014/annotation1961302/range1961302/1

https://synbiohub.org/public/igem/BBa_J45014/annotation1961302/range1961302/1
type: Range
persistent identity: range1961302
display id: range1961302
version: 1
direction: direction/forward
top level: ATF1
start: 1576
end: 1581
orientation: inline

JAMT
type: ComponentDefinition
persistent identity: BBa J45003
display id: BBa_J45003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: SAM:jasmonic acid carboxyl methyltransferase; converts jasmonic acid to jasmine odor
creator: Stephen Payne
created: 2006-06-06T11:00:00Z
modified: 2015-08-31T04:08:49Z
discontinued: false
dominant: true
experience: experience/None
group u list: _84_
m user id: 0
owner id: 642
owning group id: 84
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: This ORF encodes the enzyme responsible for catalyzing the following reaction: Jasmonic Acid--->Methyl Jasmonate It is initially found in ''Arabidopsis thaliana'' but has been transferred successfully to the E. coli genome by Seo, et al. The product, methyl jasmonate has a jasmine smell to it.
mutable notes: Km: 38.5 ??M; Kcat:25 S−1 for Jasmonic Acid--->Methyl Jasmonate with the aid of JAMT: Jasmonic acid carboxyl methyltransferase
mutable provenance: If the part becomes physically available, it would probably be donated by Seo, et al.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: JAMT
sequence annotation: start codon
sequence annotation: stop codon
sequence: https://synbiohub.org/public/igem/BBa_J45003_sequence/1

JAMT
type: SequenceAnnotation
persistent identity: annotation1961372
display id: annotation1961372
version: 1
top level: JAMT
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J45003/annotation1961372/range1961372/1

https://synbiohub.org/public/igem/BBa_J45003/annotation1961372/range1961372/1
type: Range
persistent identity: range1961372
display id: range1961372
version: 1
direction: direction/forward
top level: JAMT
start: 1
end: 1168
orientation: inline

start codon
type: SequenceAnnotation
persistent identity: annotation1961373
display id: annotation1961373
version: 1
top level: JAMT
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_J45003/annotation1961373/range1961373/1

https://synbiohub.org/public/igem/BBa_J45003/annotation1961373/range1961373/1
type: Range
persistent identity: range1961373
display id: range1961373
version: 1
direction: direction/forward
top level: JAMT
start: 1
end: 3
orientation: inline

stop codon
type: SequenceAnnotation
persistent identity: annotation1961374
display id: annotation1961374
version: 1
top level: JAMT
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_J45003/annotation1961374/range1961374/1

https://synbiohub.org/public/igem/BBa_J45003/annotation1961374/range1961374/1
type: Range
persistent identity: range1961374
display id: range1961374
version: 1
direction: direction/forward
top level: JAMT
start: 1168
end: 1170
orientation: inline

YYC912
type: ComponentDefinition
persistent identity: BBa J45999
display id: BBa_J45999
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: E. coli strain YYC912; Odor-free chassis
creator: MIT iGEM 2006
created: 2006-10-30T12:00:00Z
modified: 2015-08-31T04:08:50Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _84_
m user id: 0
owner id: 126
owning group id: 70
sample status: No part sequence
status: status/Unavailable
bookmark: true
mutable description: Escherichia coli strain YYC912. Biosafety level 1 organism.
mutable notes: None.
mutable provenance: This strain was obtained from the E.coli Genetic Resources: CGSC, The Coli Genetic Stock Center http://cgsc.biology.yale.edu/. Strain YYC912.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Cell
role: SO:0000340

BSMT1
type: ComponentDefinition
persistent identity: BBa J45119
display id: BBa_J45119
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Wintergreen odor enzyme (BMST1) generator
creator: Boyuan Zhu
created: 2006-08-09T11:00:00Z
modified: 2015-08-31T04:08:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _84_
m user id: 0
owner id: 974
owning group id: 84
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This composite part include a ribosome binding site (B0032 different from J45099), the BSMT coding region, and a terminator. This part is attached to different promoters.
mutable notes: Used different RBS.
mutable provenance: Derived from the early part J45098 and sister to J45099.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0010
component: BBa_J45004
component: BBa_B0012
component: BBa_B0032
sequence annotation: BBa_B0012
sequence annotation: BBa_J45004
sequence annotation: BBa_B0010
sequence annotation: BBa_B0032
sequence: https://synbiohub.org/public/igem/BBa_J45119_sequence/1

BBa_B0010
type: Component
persistent identity: component1894759
display id: component1894759
version: 1
top level: BSMT1
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_J45004
type: Component
persistent identity: component1894758
display id: component1894758
version: 1
top level: BSMT1
access: public
definition: BSMT1

BBa_B0012
type: Component
persistent identity: component1894761
display id: component1894761
version: 1
top level: BSMT1
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0032
type: Component
persistent identity: component1894753
display id: component1894753
version: 1
top level: BSMT1
access: public
definition: https://synbiohub.org/public/igem/BBa_B0032/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1894761
display id: annotation1894761
version: 1
top level: BSMT1
location: https://synbiohub.org/public/igem/BBa_J45119/annotation1894761/range1894761/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_J45119/annotation1894761/range1894761/1
type: Range
persistent identity: range1894761
display id: range1894761
version: 1
direction: direction/forward
top level: BSMT1
start: 1190
end: 1230
orientation: inline

BBa_J45004
type: SequenceAnnotation
persistent identity: annotation1894758
display id: annotation1894758
version: 1
top level: BSMT1
location: https://synbiohub.org/public/igem/BBa_J45119/annotation1894758/range1894758/1
component: BBa_J45004

https://synbiohub.org/public/igem/BBa_J45119/annotation1894758/range1894758/1
type: Range
persistent identity: range1894758
display id: range1894758
version: 1
direction: direction/reverse
top level: BSMT1
start: 20
end: 1093
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1894759
display id: annotation1894759
version: 1
top level: BSMT1
location: https://synbiohub.org/public/igem/BBa_J45119/annotation1894759/range1894759/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_J45119/annotation1894759/range1894759/1
type: Range
persistent identity: range1894759
display id: range1894759
version: 1
direction: direction/forward
top level: BSMT1
start: 1102
end: 1181
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation1894753
display id: annotation1894753
version: 1
top level: BSMT1
location: https://synbiohub.org/public/igem/BBa_J45119/annotation1894753/range1894753/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_J45119/annotation1894753/range1894753/1
type: Range
persistent identity: range1894753
display id: range1894753
version: 1
direction: direction/forward
top level: BSMT1
start: 1
end: 13
orientation: inline

BAMT
type: ComponentDefinition
persistent identity: BBa J45002
display id: BBa_J45002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: SAM:benzoic acid carboxyl methyltransferase; converts benzoic acid to methyl benzoate (floral odor)
creator: Andr?? Green II
created: 2006-06-06T11:00:00Z
modified: 2015-08-31T04:08:49Z
discontinued: false
dominant: false
experience: experience/Fails
group u list: _84_
m user id: 0
owner id: 977
owning group id: 84
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: BAMT catalyzes the transfer of a methyl group from SAM (S-adenosyl methyltransferase) to benzoic acid, creating methyl benzoate. Methyl benzoate produces a "pleasant" smell.
mutable notes: none as of yet
mutable provenance: Genbank accession number(s): AF198492 This part is from A. majus.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: BAMT
sequence annotation: putative start codon 2
sequence annotation: putative start codon 1
sequence annotation: A -> G to eliminate SpeI site
sequence annotation: double TAA stop codon
sequence: https://synbiohub.org/public/igem/BBa_J45002_sequence/1

BAMT
type: SequenceAnnotation
persistent identity: annotation1880494
display id: annotation1880494
version: 1
top level: BAMT
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J45002/annotation1880494/range1880494/1

https://synbiohub.org/public/igem/BBa_J45002/annotation1880494/range1880494/1
type: Range
persistent identity: range1880494
display id: range1880494
version: 1
direction: direction/forward
top level: BAMT
start: 1
end: 1095
orientation: inline

putative start codon 2
type: SequenceAnnotation
persistent identity: annotation1880476
display id: annotation1880476
version: 1
top level: BAMT
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_J45002/annotation1880476/range1880476/1

https://synbiohub.org/public/igem/BBa_J45002/annotation1880476/range1880476/1
type: Range
persistent identity: range1880476
display id: range1880476
version: 1
direction: direction/forward
top level: BAMT
start: 10
end: 12
orientation: inline

putative start codon 1
type: SequenceAnnotation
persistent identity: annotation1880475
display id: annotation1880475
version: 1
top level: BAMT
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_J45002/annotation1880475/range1880475/1

https://synbiohub.org/public/igem/BBa_J45002/annotation1880475/range1880475/1
type: Range
persistent identity: range1880475
display id: range1880475
version: 1
direction: direction/forward
top level: BAMT
start: 1
end: 3
orientation: inline

A -> G to eliminate SpeI site
type: SequenceAnnotation
persistent identity: annotation1880506
display id: annotation1880506
version: 1
top level: BAMT
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_J45002/annotation1880506/range1880506/1

https://synbiohub.org/public/igem/BBa_J45002/annotation1880506/range1880506/1
type: Range
persistent identity: range1880506
display id: range1880506
version: 1
direction: direction/forward
top level: BAMT
start: 702
end: 702
orientation: inline

double TAA stop codon
type: SequenceAnnotation
persistent identity: annotation1880495
display id: annotation1880495
version: 1
top level: BAMT
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_J45002/annotation1880495/range1880495/1

https://synbiohub.org/public/igem/BBa_J45002/annotation1880495/range1880495/1
type: Range
persistent identity: range1880495
display id: range1880495
version: 1
direction: direction/forward
top level: BAMT
start: 1093
end: 1098
orientation: inline

SG
type: ComponentDefinition
persistent identity: BBa J45320
display id: BBa_J45320
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Salicylate generator
creator: Andr?? Green II
created: 2006-10-19T11:00:00Z
modified: 2015-08-31T04:08:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _84_
m user id: 0
owner id: 977
owning group id: 84
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: Protein generator converting PoPS to the prot BSMT. The BSMT enzyme converts benzoic acid/salicylic acid to methyl benzoate and methyl salicylate.
mutable notes: None
mutable provenance: This part is phBSMT1 from Petunia x hybrida (GenBank). See part design and discussion for more information.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0012
component: BBa_R0011
component: BBa_B0032
component: BBa_J45017
component: BBa_B0010
sequence annotation: BBa_J45017
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_R0011
sequence annotation: BBa_B0032
sequence: https://synbiohub.org/public/igem/BBa_J45320_sequence/1

BBa_B0012
type: Component
persistent identity: component1960961
display id: component1960961
version: 1
top level: SG
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_R0011
type: Component
persistent identity: component1960945
display id: component1960945
version: 1
top level: SG
access: public
definition: https://synbiohub.org/public/igem/BBa_R0011/1

BBa_B0032
type: Component
persistent identity: component1960951
display id: component1960951
version: 1
top level: SG
access: public
definition: https://synbiohub.org/public/igem/BBa_B0032/1

BBa_J45017
type: Component
persistent identity: component1960958
display id: component1960958
version: 1
top level: SG
access: public
definition: pchBA

BBa_B0010
type: Component
persistent identity: component1960959
display id: component1960959
version: 1
top level: SG
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_J45017
type: SequenceAnnotation
persistent identity: annotation1960958
display id: annotation1960958
version: 1
top level: SG
location: https://synbiohub.org/public/igem/BBa_J45320/annotation1960958/range1960958/1
component: BBa_J45017

https://synbiohub.org/public/igem/BBa_J45320/annotation1960958/range1960958/1
type: Range
persistent identity: range1960958
display id: range1960958
version: 1
direction: direction/reverse
top level: SG
start: 83
end: 1818
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1960959
display id: annotation1960959
version: 1
top level: SG
location: https://synbiohub.org/public/igem/BBa_J45320/annotation1960959/range1960959/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_J45320/annotation1960959/range1960959/1
type: Range
persistent identity: range1960959
display id: range1960959
version: 1
direction: direction/forward
top level: SG
start: 1827
end: 1906
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1960961
display id: annotation1960961
version: 1
top level: SG
location: https://synbiohub.org/public/igem/BBa_J45320/annotation1960961/range1960961/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_J45320/annotation1960961/range1960961/1
type: Range
persistent identity: range1960961
display id: range1960961
version: 1
direction: direction/forward
top level: SG
start: 1915
end: 1955
orientation: inline

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation1960945
display id: annotation1960945
version: 1
top level: SG
location: https://synbiohub.org/public/igem/BBa_J45320/annotation1960945/range1960945/1
component: BBa_R0011

https://synbiohub.org/public/igem/BBa_J45320/annotation1960945/range1960945/1
type: Range
persistent identity: range1960945
display id: range1960945
version: 1
direction: direction/forward
top level: SG
start: 1
end: 54
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation1960951
display id: annotation1960951
version: 1
top level: SG
location: https://synbiohub.org/public/igem/BBa_J45320/annotation1960951/range1960951/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_J45320/annotation1960951/range1960951/1
type: Range
persistent identity: range1960951
display id: range1960951
version: 1
direction: direction/forward
top level: SG
start: 64
end: 76
orientation: inline

WOG
type: ComponentDefinition
persistent identity: BBa J45120
display id: BBa_J45120
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Wintergreen odor generator
creator: MIT IGEM 2006
created: 2006-08-09T11:00:00Z
modified: 2015-08-31T04:08:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _84_
m user id: 0
owner id: 1147
owning group id: 84
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This is the wintergreen generating device hooked up to a constitutive promoter with a different RBS (B0032, different from 45100).
mutable notes: N/A
mutable provenance: Built off biobricks R0040 and 45119.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_J45004
component: BBa_B0012
component: BBa_B0032
component: BBa_R0040
component: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_B0032
sequence annotation: BBa_R0040
sequence annotation: BBa_J45004
sequence annotation: BBa_B0010
sequence: https://synbiohub.org/public/igem/BBa_J45120_sequence/1

BBa_J45004
type: Component
persistent identity: component1894776
display id: component1894776
version: 1
top level: WOG
access: public
definition: BSMT1

BBa_B0012
type: Component
persistent identity: component1894779
display id: component1894779
version: 1
top level: WOG
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0032
type: Component
persistent identity: component1894771
display id: component1894771
version: 1
top level: WOG
access: public
definition: https://synbiohub.org/public/igem/BBa_B0032/1

BBa_R0040
type: Component
persistent identity: component1894766
display id: component1894766
version: 1
top level: WOG
access: public
definition: https://synbiohub.org/public/igem/BBa_R0040/1

BBa_B0010
type: Component
persistent identity: component1894777
display id: component1894777
version: 1
top level: WOG
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1894779
display id: annotation1894779
version: 1
top level: WOG
location: https://synbiohub.org/public/igem/BBa_J45120/annotation1894779/range1894779/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_J45120/annotation1894779/range1894779/1
type: Range
persistent identity: range1894779
display id: range1894779
version: 1
direction: direction/forward
top level: WOG
start: 1252
end: 1292
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation1894771
display id: annotation1894771
version: 1
top level: WOG
location: https://synbiohub.org/public/igem/BBa_J45120/annotation1894771/range1894771/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_J45120/annotation1894771/range1894771/1
type: Range
persistent identity: range1894771
display id: range1894771
version: 1
direction: direction/forward
top level: WOG
start: 63
end: 75
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1894766
display id: annotation1894766
version: 1
top level: WOG
location: https://synbiohub.org/public/igem/BBa_J45120/annotation1894766/range1894766/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_J45120/annotation1894766/range1894766/1
type: Range
persistent identity: range1894766
display id: range1894766
version: 1
direction: direction/forward
top level: WOG
start: 1
end: 54
orientation: inline

BBa_J45004
type: SequenceAnnotation
persistent identity: annotation1894776
display id: annotation1894776
version: 1
top level: WOG
location: https://synbiohub.org/public/igem/BBa_J45120/annotation1894776/range1894776/1
component: BBa_J45004

https://synbiohub.org/public/igem/BBa_J45120/annotation1894776/range1894776/1
type: Range
persistent identity: range1894776
display id: range1894776
version: 1
direction: direction/reverse
top level: WOG
start: 82
end: 1155
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1894777
display id: annotation1894777
version: 1
top level: WOG
location: https://synbiohub.org/public/igem/BBa_J45120/annotation1894777/range1894777/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_J45120/annotation1894777/range1894777/1
type: Range
persistent identity: range1894777
display id: range1894777
version: 1
direction: direction/forward
top level: WOG
start: 1164
end: 1243
orientation: inline

BBa_K118003
type: ComponentDefinition
persistent identity: BBa K118003
display id: BBa_K118003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: crtI coding sequence encoding phytoene dehydrogenase
creator: Andrew Hall
created: 2008-07-23T11:00:00Z
modified: 2015-05-08T01:09:36Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _192_
m user id: 0
owner id: 3282
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: This is the coding sequence of crtI from Pantoea ananatis (formerly Erwinia uredovora) (Accession number D90087). It encodes phytoene dehydrogenase, part of the carotenoid biosynthesis pathway, which converts Phytoene to Lycopene.
mutable notes: The native crtI sequence contains two internal PstI sites. Primers crtImutf1 and crtImutf2 were ordered so that the MABEL protocol (invented for this purpose) could be used to remove these sites. The mutagenic primers were as follows: * crtImutf1: gtt tac agt aag gtt gcc * crtImutf2: agt aac tct ctg ttt gtg c In both cases, the PstI site CTGCAG was converted to CTGCAA, a silent mutation.
mutable provenance: Pantoea ananatis genomic DNA
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: PstI site removed
sequence annotation: crtI coding sequence
sequence: https://synbiohub.org/public/igem/BBa_K118003_sequence/1

PstI site removed
type: SequenceAnnotation
persistent identity: annotation1968244
display id: annotation1968244
version: 1
top level: BBa_K118003
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_K118003/annotation1968244/range1968244/1

https://synbiohub.org/public/igem/BBa_K118003/annotation1968244/range1968244/1
type: Range
persistent identity: range1968244
display id: range1968244
version: 1
direction: direction/forward
top level: BBa_K118003
start: 478
end: 483
orientation: inline

crtI coding sequence
type: SequenceAnnotation
persistent identity: annotation1995702
display id: annotation1995702
version: 1
top level: BBa_K118003
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K118003/annotation1995702/range1995702/1

https://synbiohub.org/public/igem/BBa_K118003/annotation1995702/range1995702/1
type: Range
persistent identity: range1995702
display id: range1995702
version: 1
direction: direction/forward
top level: BBa_K118003
start: 1
end: 1479
orientation: inline

PstI site removed
type: SequenceAnnotation
persistent identity: annotation1968245
display id: annotation1968245
version: 1
top level: BBa_K118003
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_K118003/annotation1968245/range1968245/1

https://synbiohub.org/public/igem/BBa_K118003/annotation1968245/range1968245/1
type: Range
persistent identity: range1968245
display id: range1968245
version: 1
direction: direction/forward
top level: BBa_K118003
start: 889
end: 894
orientation: inline

Wintergree
type: ComponentDefinition
persistent identity: BBa J45181
display id: BBa_J45181
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Exponential phase dependent wintergreen odor generator
creator: Stephen Payne
created: 2007-02-10T12:00:00Z
modified: 2015-08-31T04:08:49Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _84_
m user id: 0
owner id: 642
owning group id: 84
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This part converts salicylic acid into methyl salicylate (a mint-smelling compound) in the exponential phase of cell growth. The part consists of a stationary phase promoter (J45992), connected to an inverter (Q04401) as well as the methyl salicylate protein generator (J45119). This part replaces the defective part J45180, which used the truncated version of the stationary phase promoter (J45993). This part did not function as it was designed due to an imbalance between the strength of the stationary phase promoter and the strength of the promoter in the inverter (R0040).
mutable notes: We needed the full osmY promoter strength to be relatively comparable to the R0040 promoter strength.
mutable provenance: Yes. It comes from many subparts.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_J45994
component: BBa_J45119
sequence annotation: BBa_J45119
sequence annotation: BBa_J45994
sequence: https://synbiohub.org/public/igem/BBa_J45181_sequence/1

BBa_J45994
type: Component
persistent identity: component2260476
display id: component2260476
version: 1
top level: Wintergree
access: public
definition: https://synbiohub.org/public/igem/BBa_J45994/1

BBa_J45119
type: Component
persistent identity: component2260490
display id: component2260490
version: 1
top level: Wintergree
access: public
definition: BSMT1

BBa_J45119
type: SequenceAnnotation
persistent identity: annotation2260490
display id: annotation2260490
version: 1
top level: Wintergree
location: https://synbiohub.org/public/igem/BBa_J45181/annotation2260490/range2260490/1
component: BBa_J45119

https://synbiohub.org/public/igem/BBa_J45181/annotation2260490/range2260490/1
type: Range
persistent identity: range2260490
display id: range2260490
version: 1
direction: direction/reverse
top level: Wintergree
start: 1118
end: 2347
orientation: inline

BBa_J45994
type: SequenceAnnotation
persistent identity: annotation2260476
display id: annotation2260476
version: 1
top level: Wintergree
location: https://synbiohub.org/public/igem/BBa_J45181/annotation2260476/range2260476/1
component: BBa_J45994

https://synbiohub.org/public/igem/BBa_J45181/annotation2260476/range2260476/1
type: Range
persistent identity: range2260476
display id: range2260476
version: 1
direction: direction/reverse
top level: Wintergree
start: 1
end: 1109
orientation: inline

BBa_J45270
type: ComponentDefinition
persistent identity: BBa J45270
display id: BBa_J45270
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Stationary-phase-dependent banana odor generator
creator: Andr?? Green II
created: 2006-10-27T11:00:00Z
modified: 2015-08-31T04:08:49Z
discontinued: false
dominant: false
experience: experience/None
group u list: _84_
m user id: 0
owner id: 977
owning group id: 84
sample status: In stock
status: status/Available
bookmark: false
mutable description: This part is an ATF1-1148 protein generator under control by the osmY stationary phase promoter (BBa_J45993). It is almost identical to J45250, except that: 1. The promoter is the truncated version of osmY and 2. it uses the B0030 RBS instead of B0032 (which is in J45250).
mutable notes: none
mutable provenance: coming
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0012
component: BBa_J45014
component: BBa_B0010
component: BBa_J45993
component: BBa_B0032
sequence annotation: BBa_J45993
sequence annotation: BBa_B0012
sequence annotation: BBa_B0010
sequence annotation: BBa_B0032
sequence annotation: BBa_J45014
sequence: https://synbiohub.org/public/igem/BBa_J45270_sequence/1

BBa_B0012
type: Component
persistent identity: component1908100
display id: component1908100
version: 1
top level: BBa_J45270
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_J45014
type: Component
persistent identity: component1908097
display id: component1908097
version: 1
top level: BBa_J45270
access: public
definition: ATF1

BBa_B0010
type: Component
persistent identity: component1908098
display id: component1908098
version: 1
top level: BBa_J45270
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_J45993
type: Component
persistent identity: component1908089
display id: component1908089
version: 1
top level: BBa_J45270
access: public
definition: https://synbiohub.org/public/igem/BBa_J45993/1

BBa_B0032
type: Component
persistent identity: component1908091
display id: component1908091
version: 1
top level: BBa_J45270
access: public
definition: https://synbiohub.org/public/igem/BBa_B0032/1

BBa_J45993
type: SequenceAnnotation
persistent identity: annotation1908089
display id: annotation1908089
version: 1
top level: BBa_J45270
location: https://synbiohub.org/public/igem/BBa_J45270/annotation1908089/range1908089/1
component: BBa_J45993

https://synbiohub.org/public/igem/BBa_J45270/annotation1908089/range1908089/1
type: Range
persistent identity: range1908089
display id: range1908089
version: 1
direction: direction/reverse
top level: BBa_J45270
start: 1
end: 57
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1908100
display id: annotation1908100
version: 1
top level: BBa_J45270
location: https://synbiohub.org/public/igem/BBa_J45270/annotation1908100/range1908100/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_J45270/annotation1908100/range1908100/1
type: Range
persistent identity: range1908100
display id: range1908100
version: 1
direction: direction/forward
top level: BBa_J45270
start: 1762
end: 1802
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1908098
display id: annotation1908098
version: 1
top level: BBa_J45270
location: https://synbiohub.org/public/igem/BBa_J45270/annotation1908098/range1908098/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_J45270/annotation1908098/range1908098/1
type: Range
persistent identity: range1908098
display id: range1908098
version: 1
direction: direction/forward
top level: BBa_J45270
start: 1674
end: 1753
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation1908091
display id: annotation1908091
version: 1
top level: BBa_J45270
location: https://synbiohub.org/public/igem/BBa_J45270/annotation1908091/range1908091/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_J45270/annotation1908091/range1908091/1
type: Range
persistent identity: range1908091
display id: range1908091
version: 1
direction: direction/forward
top level: BBa_J45270
start: 66
end: 78
orientation: inline

BBa_J45014
type: SequenceAnnotation
persistent identity: annotation1908097
display id: annotation1908097
version: 1
top level: BBa_J45270
location: https://synbiohub.org/public/igem/BBa_J45270/annotation1908097/range1908097/1
component: BBa_J45014

https://synbiohub.org/public/igem/BBa_J45270/annotation1908097/range1908097/1
type: Range
persistent identity: range1908097
display id: range1908097
version: 1
direction: direction/reverse
top level: BBa_J45270
start: 85
end: 1665
orientation: inline

ATF1
type: ComponentDefinition
persistent identity: BBa J45006
display id: BBa_J45006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: alcohol acetyltransferase I (ATF1)
creator: Veena Venkatachalam
created: 2006-06-06T11:00:00Z
modified: 2015-08-31T04:08:49Z
discontinued: false
dominant: false
experience: experience/None
group u list: _84_
m user id: 0
owner id: 975
owning group id: 84
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This enzyme catalyzes the reaction of isoamyl alcohol -> isoamyl acetate
mutable notes: n/a
mutable provenance: Sequence obtained from Genbank; #Z75285
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: silent mutation to eliminate EcoRI site
sequence annotation: coding region
sequence annotation: start
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_J45006_sequence/1

silent mutation to eliminate EcoRI site
type: SequenceAnnotation
persistent identity: annotation1880547
display id: annotation1880547
version: 1
top level: ATF1
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_J45006/annotation1880547/range1880547/1

https://synbiohub.org/public/igem/BBa_J45006/annotation1880547/range1880547/1
type: Range
persistent identity: range1880547
display id: range1880547
version: 1
direction: direction/forward
top level: ATF1
start: 414
end: 414
orientation: inline

coding region
type: SequenceAnnotation
persistent identity: annotation1880545
display id: annotation1880545
version: 1
top level: ATF1
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J45006/annotation1880545/range1880545/1

https://synbiohub.org/public/igem/BBa_J45006/annotation1880545/range1880545/1
type: Range
persistent identity: range1880545
display id: range1880545
version: 1
direction: direction/forward
top level: ATF1
start: 1
end: 1581
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation1880546
display id: annotation1880546
version: 1
top level: ATF1
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_J45006/annotation1880546/range1880546/1

https://synbiohub.org/public/igem/BBa_J45006/annotation1880546/range1880546/1
type: Range
persistent identity: range1880546
display id: range1880546
version: 1
direction: direction/forward
top level: ATF1
start: 1
end: 3
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1880548
display id: annotation1880548
version: 1
top level: ATF1
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_J45006/annotation1880548/range1880548/1

https://synbiohub.org/public/igem/BBa_J45006/annotation1880548/range1880548/1
type: Range
persistent identity: range1880548
display id: range1880548
version: 1
direction: direction/forward
top level: ATF1
start: 1576
end: 1581
orientation: inline

ATF1 gener
type: ComponentDefinition
persistent identity: BBa J45199
display id: BBa_J45199
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Banana odor enzyme (ATF1) generator
creator: Andr?? Green II
created: 2006-10-27T11:00:00Z
modified: 2015-08-31T04:08:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _84_
m user id: 0
owner id: 977
owning group id: 84
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: This part is an intermediate to the protein generator BBa_45200.
mutable notes: coming
mutable provenance: coming
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0012
component: BBa_B0030
component: BBa_B0010
component: BBa_J45014
sequence annotation: BBa_B0030
sequence annotation: BBa_B0012
sequence annotation: BBa_J45014
sequence annotation: BBa_B0010
sequence: https://synbiohub.org/public/igem/BBa_J45199_sequence/1

BBa_B0012
type: Component
persistent identity: component1908018
display id: component1908018
version: 1
top level: ATF1 gener
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0030
type: Component
persistent identity: component1908009
display id: component1908009
version: 1
top level: ATF1 gener
access: public
definition: https://synbiohub.org/public/igem/BBa_B0030/1

BBa_B0010
type: Component
persistent identity: component1908016
display id: component1908016
version: 1
top level: ATF1 gener
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_J45014
type: Component
persistent identity: component1908015
display id: component1908015
version: 1
top level: ATF1 gener
access: public
definition: ATF1

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation1908009
display id: annotation1908009
version: 1
top level: ATF1 gener
location: https://synbiohub.org/public/igem/BBa_J45199/annotation1908009/range1908009/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_J45199/annotation1908009/range1908009/1
type: Range
persistent identity: range1908009
display id: range1908009
version: 1
direction: direction/forward
top level: ATF1 gener
start: 1
end: 15
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1908018
display id: annotation1908018
version: 1
top level: ATF1 gener
location: https://synbiohub.org/public/igem/BBa_J45199/annotation1908018/range1908018/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_J45199/annotation1908018/range1908018/1
type: Range
persistent identity: range1908018
display id: range1908018
version: 1
direction: direction/forward
top level: ATF1 gener
start: 1699
end: 1739
orientation: inline

BBa_J45014
type: SequenceAnnotation
persistent identity: annotation1908015
display id: annotation1908015
version: 1
top level: ATF1 gener
location: https://synbiohub.org/public/igem/BBa_J45199/annotation1908015/range1908015/1
component: BBa_J45014

https://synbiohub.org/public/igem/BBa_J45199/annotation1908015/range1908015/1
type: Range
persistent identity: range1908015
display id: range1908015
version: 1
direction: direction/reverse
top level: ATF1 gener
start: 22
end: 1602
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1908016
display id: annotation1908016
version: 1
top level: ATF1 gener
location: https://synbiohub.org/public/igem/BBa_J45199/annotation1908016/range1908016/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_J45199/annotation1908016/range1908016/1
type: Range
persistent identity: range1908016
display id: range1908016
version: 1
direction: direction/forward
top level: ATF1 gener
start: 1611
end: 1690
orientation: inline

COMT
type: ComponentDefinition
persistent identity: BBa I742107
display id: BBa_I742107
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: COMT
creator: sarah hollingshead
created: 2007-10-18T11:00:00Z
modified: 2015-08-31T04:08:02Z
discontinued: false
dominant: false
experience: experience/None
group u list: _123_
m user id: 0
owner id: 1964
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: COMT coding sequence Encodes caffeic acid-O-methyl transferase (methylates -OH on C4 of the aromatic ring) from Medicago sativa (alfalfa), synthesised by Geneart
mutable notes: Used cDNA as coding gene in Alfalfa genome contains introns.
mutable provenance: Alfalfa cDNA Genes and Enzymes Involved in Caffeic Acid Biosynthesis in the Actinomycete Saccharothrix esanaensis, Berner M, et al, Journal of Bacteriology 188(7): 2666, (2006) Gowri G, et al, Molecular cloning and expression of alfalfa S-adenosyl-L-methionine: caffeic acid 3-0-methyltransferase, a key enzyme of lignin biosynthesis, Plant Physiology 97(1):7 (1991) Downregulation of Caffeic Acid 3-O-Methyltransferase and Caffeoyl CoA 3-O-Methyltransferase in Transgenic Alfalfa Impacts on Lignin Structure and Implications for the Biosynthesis of G and S Lignin, Guo D, et al, Plant Cell. January; 13(1): 73 (2001)
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: COMT coding region
sequence: https://synbiohub.org/public/igem/BBa_I742107_sequence/1

COMT coding region
type: SequenceAnnotation
persistent identity: annotation1949817
display id: annotation1949817
version: 1
top level: COMT
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_I742107/annotation1949817/range1949817/1

https://synbiohub.org/public/igem/BBa_I742107/annotation1949817/range1949817/1
type: Range
persistent identity: range1949817
display id: range1949817
version: 1
direction: direction/forward
top level: COMT
start: 1
end: 1101
orientation: inline

BOG
type: ComponentDefinition
persistent identity: BBa J45250
display id: BBa_J45250
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Stationary-phase-dependent banana odor generator
creator: Andr?? Green II
created: 2006-10-27T11:00:00Z
modified: 2015-08-31T04:08:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _84_
m user id: 0
owner id: 977
owning group id: 84
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This part is an ATF1-1148 protein generator under control by the osmY stationary phase promoter. It is almost identical to J45270, except that: 1) The promoter is the truncated version of osmY and 2) it uses the B0030 RBS instead of B0032 (which is in J45270).
mutable notes: coming
mutable provenance: coming
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_J45992
component: BBa_B0012
component: BBa_B0030
component: BBa_B0010
component: BBa_J45014
sequence annotation: BBa_B0030
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_J45014
sequence annotation: BBa_J45992
sequence: https://synbiohub.org/public/igem/BBa_J45250_sequence/1

BBa_J45992
type: Component
persistent identity: component1908072
display id: component1908072
version: 1
top level: BOG
access: public
definition: https://synbiohub.org/public/igem/BBa_J45992/1

BBa_B0012
type: Component
persistent identity: component1908083
display id: component1908083
version: 1
top level: BOG
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0030
type: Component
persistent identity: component1908074
display id: component1908074
version: 1
top level: BOG
access: public
definition: https://synbiohub.org/public/igem/BBa_B0030/1

BBa_B0010
type: Component
persistent identity: component1908081
display id: component1908081
version: 1
top level: BOG
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_J45014
type: Component
persistent identity: component1908080
display id: component1908080
version: 1
top level: BOG
access: public
definition: ATF1

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation1908074
display id: annotation1908074
version: 1
top level: BOG
location: https://synbiohub.org/public/igem/BBa_J45250/annotation1908074/range1908074/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_J45250/annotation1908074/range1908074/1
type: Range
persistent identity: range1908074
display id: range1908074
version: 1
direction: direction/forward
top level: BOG
start: 208
end: 222
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1908081
display id: annotation1908081
version: 1
top level: BOG
location: https://synbiohub.org/public/igem/BBa_J45250/annotation1908081/range1908081/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_J45250/annotation1908081/range1908081/1
type: Range
persistent identity: range1908081
display id: range1908081
version: 1
direction: direction/forward
top level: BOG
start: 1818
end: 1897
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1908083
display id: annotation1908083
version: 1
top level: BOG
location: https://synbiohub.org/public/igem/BBa_J45250/annotation1908083/range1908083/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_J45250/annotation1908083/range1908083/1
type: Range
persistent identity: range1908083
display id: range1908083
version: 1
direction: direction/forward
top level: BOG
start: 1906
end: 1946
orientation: inline

BBa_J45014
type: SequenceAnnotation
persistent identity: annotation1908080
display id: annotation1908080
version: 1
top level: BOG
location: https://synbiohub.org/public/igem/BBa_J45250/annotation1908080/range1908080/1
component: BBa_J45014

https://synbiohub.org/public/igem/BBa_J45250/annotation1908080/range1908080/1
type: Range
persistent identity: range1908080
display id: range1908080
version: 1
direction: direction/reverse
top level: BOG
start: 229
end: 1809
orientation: inline

BBa_J45992
type: SequenceAnnotation
persistent identity: annotation1908072
display id: annotation1908072
version: 1
top level: BOG
location: https://synbiohub.org/public/igem/BBa_J45250/annotation1908072/range1908072/1
component: BBa_J45992

https://synbiohub.org/public/igem/BBa_J45250/annotation1908072/range1908072/1
type: Range
persistent identity: range1908072
display id: range1908072
version: 1
direction: direction/reverse
top level: BOG
start: 1
end: 199
orientation: inline

rbs+LIMS1
type: ComponentDefinition
persistent identity: BBa I742111
display id: BBa_I742111
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Limonene synthase gene with ribosome binding site
creator: sarah hollingshead
created: 2007-10-18T11:00:00Z
modified: 2015-08-31T04:08:02Z
discontinued: false
dominant: false
experience: experience/None
group u list: _123_
m user id: 0
owner id: 1964
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: Ribosome binding site attached to Limonene synthase gene. Limonene synthase enzyme produces (+)-limonene from farnesyl diphosphate (intermediate in mevlonate pathway). (+) Limonene is a component of lemon scent.
mutable notes: several codons were altered silently to remove several forbidden restriction sites. The N-terminal region (plastid targeting sequence) was removed, removing the first 51 amino acids and replacing codon 52 with the start codon ATG. LIMS cDNA was used as genomic DNA contains introns.
mutable provenance: Monoterpene biosynthesis in lemon (Citrus limon) cDNA isolation and functional analysis of four monoterpene synthases, J Lucker, et al, Eur. J. Biochem. 269: 3160???3171 (2002) Analysis of Lemon and Bergamot Essential Oils by HPLC with Microbore Columns, M Benincasa, et al, Chromatographia 30(5/6):271 (1990) Citrus fruit flavour and aroma biosynthesis, L Sharon-Asa, The Plant Journal, 36:664 (2003)
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_I742110
component: BBa_J15001
sequence annotation: BBa_I742110
sequence annotation: BBa_J15001
sequence: https://synbiohub.org/public/igem/BBa_I742111_sequence/1

BBa_I742110
type: Component
persistent identity: component1955374
display id: component1955374
version: 1
top level: rbs+LIMS1
access: public
definition: https://synbiohub.org/public/igem/BBa_I742110/1

BBa_J15001
type: Component
persistent identity: component1955370
display id: component1955370
version: 1
top level: rbs+LIMS1
access: public
definition: https://synbiohub.org/public/igem/BBa_J15001/1

BBa_I742110
type: SequenceAnnotation
persistent identity: annotation1955374
display id: annotation1955374
version: 1
top level: rbs+LIMS1
location: https://synbiohub.org/public/igem/BBa_I742111/annotation1955374/range1955374/1
component: BBa_I742110

https://synbiohub.org/public/igem/BBa_I742111/annotation1955374/range1955374/1
type: Range
persistent identity: range1955374
display id: range1955374
version: 1
direction: direction/reverse
top level: rbs+LIMS1
start: 17
end: 1687
orientation: inline

BBa_J15001
type: SequenceAnnotation
persistent identity: annotation1955370
display id: annotation1955370
version: 1
top level: rbs+LIMS1
location: https://synbiohub.org/public/igem/BBa_I742111/annotation1955370/range1955370/1
component: BBa_J15001

https://synbiohub.org/public/igem/BBa_I742111/annotation1955370/range1955370/1
type: Range
persistent identity: range1955370
display id: range1955370
version: 1
direction: direction/reverse
top level: rbs+LIMS1
start: 1
end: 10
orientation: inline

BBa_K1919002
type: ComponentDefinition
persistent identity: BBa K1919002
display id: BBa_K1919002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Constitutive promotor J23106 and downstream CecropinXJ
creator: Shiji Zhao
created: 2016-09-04T11:00:00Z
modified: 2016-10-14T08:27:47Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2386_
m user id: 26103
owner id: 26103
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Biobrick BBa_K1919002 is a composite part, consisting of constitutive promotor J23106, RBS, TrxA tag, 6xHis tag, thrombin site, S tag, enterkinase site and the coding sequence of antimicrobial peptide CecropinXJ.
mutable notes: Two steps were needed in order to obtain BBa_K1919006. Step 1, BBa_K1919000 was inserted into pET32a(+). The primers used for PCR are as following: F: 5'-CCGGGATCCATGAACTTCGCTAAAATCCTGTCTT-3' (BamH I site was added) R: 5'-CCGCTCGAGTTATTATTTACCGATAGCTTTA-3' (Xho I site was added) BamH I/Xho I Double enzymatic cutting was applied for both pET32a(+) and BBa_K1919000. Step 2, RBS and 5 downstream tags as well as original CecropinXJ was inserted at the downstream of promotor J23106 with the same procedure as step 1. The primers used for PCR are as following: F: 5'-GCTCTAGAGCGAAGGAGATATACATATGAGCG-3' (Xba I site was added) R: 5'-TGCACTGCAGTGCAGACTAGTCGTTATTGCTCAGCGGTGG-3'(Pst I site was added) Xba I/Pst I double enzymatic cutting was applied for PCR product and Spe I/Pst I double enzymatic cutting was used for BBa_J23106. After enzymatic linkage, the construction of BBa_K1919002 was finished. Then this part was transfered into pSB1C3 backbone.
mutable provenance: The sequence of constitutive promotor J23106 comes from biobrick BBa_J23106. RBS and 5 downstream tags come from commercial plasmid pET32a(+). The sequence of CecropinXJ comes from biobrick BBa_K1919000
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
sequence annotation: BBa_K1919000
sequence annotation: double TAA stop codon
sequence annotation: enterokinase site
sequence annotation: Thrombin site
sequence annotation: TrxA
sequence annotation: ATG start codon
sequence annotation: BBa_J23106
sequence annotation: CecropinXJ coding sequence
sequence annotation: RBS
sequence annotation: S-Tag
sequence annotation: 6xHis Tag
sequence: https://synbiohub.org/public/igem/BBa_K1919002_sequence/1

BBa_K1919000
type: SequenceAnnotation
persistent identity: annotation2482488
display id: annotation2482488
version: 1
top level: BBa_K1919002
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1919002/annotation2482488/range2482488/1

https://synbiohub.org/public/igem/BBa_K1919002/annotation2482488/range2482488/1
type: Range
persistent identity: range2482488
display id: range2482488
version: 1
direction: direction/forward
top level: BBa_K1919002
start: 555
end: 749
orientation: inline

double TAA stop codon
type: SequenceAnnotation
persistent identity: annotation2482489
display id: annotation2482489
version: 1
top level: BBa_K1919002
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1919002/annotation2482489/range2482489/1

https://synbiohub.org/public/igem/BBa_K1919002/annotation2482489/range2482489/1
type: Range
persistent identity: range2482489
display id: range2482489
version: 1
direction: direction/forward
top level: BBa_K1919002
start: 744
end: 749
orientation: inline

enterokinase site
type: SequenceAnnotation
persistent identity: annotation2482486
display id: annotation2482486
version: 1
top level: BBa_K1919002
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1919002/annotation2482486/range2482486/1

https://synbiohub.org/public/igem/BBa_K1919002/annotation2482486/range2482486/1
type: Range
persistent identity: range2482486
display id: range2482486
version: 1
direction: direction/forward
top level: BBa_K1919002
start: 519
end: 533
orientation: inline

Thrombin site
type: SequenceAnnotation
persistent identity: annotation2482484
display id: annotation2482484
version: 1
top level: BBa_K1919002
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1919002/annotation2482484/range2482484/1

https://synbiohub.org/public/igem/BBa_K1919002/annotation2482484/range2482484/1
type: Range
persistent identity: range2482484
display id: range2482484
version: 1
direction: direction/forward
top level: BBa_K1919002
start: 435
end: 452
orientation: inline

TrxA
type: SequenceAnnotation
persistent identity: annotation2482482
display id: annotation2482482
version: 1
top level: BBa_K1919002
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1919002/annotation2482482/range2482482/1

https://synbiohub.org/public/igem/BBa_K1919002/annotation2482482/range2482482/1
type: Range
persistent identity: range2482482
display id: range2482482
version: 1
direction: direction/forward
top level: BBa_K1919002
start: 60
end: 386
orientation: inline

ATG start codon
type: SequenceAnnotation
persistent identity: annotation2482481
display id: annotation2482481
version: 1
top level: BBa_K1919002
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1919002/annotation2482481/range2482481/1

https://synbiohub.org/public/igem/BBa_K1919002/annotation2482481/range2482481/1
type: Range
persistent identity: range2482481
display id: range2482481
version: 1
direction: direction/forward
top level: BBa_K1919002
start: 60
end: 62
orientation: inline

BBa_J23106
type: SequenceAnnotation
persistent identity: annotation2482479
display id: annotation2482479
version: 1
top level: BBa_K1919002
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1919002/annotation2482479/range2482479/1

https://synbiohub.org/public/igem/BBa_K1919002/annotation2482479/range2482479/1
type: Range
persistent identity: range2482479
display id: range2482479
version: 1
direction: direction/forward
top level: BBa_K1919002
start: 1
end: 35
orientation: inline

CecropinXJ coding sequence
type: SequenceAnnotation
persistent identity: annotation2482487
display id: annotation2482487
version: 1
top level: BBa_K1919002
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1919002/annotation2482487/range2482487/1

https://synbiohub.org/public/igem/BBa_K1919002/annotation2482487/range2482487/1
type: Range
persistent identity: range2482487
display id: range2482487
version: 1
direction: direction/forward
top level: BBa_K1919002
start: 555
end: 749
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2482480
display id: annotation2482480
version: 1
top level: BBa_K1919002
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1919002/annotation2482480/range2482480/1

https://synbiohub.org/public/igem/BBa_K1919002/annotation2482480/range2482480/1
type: Range
persistent identity: range2482480
display id: range2482480
version: 1
direction: direction/forward
top level: BBa_K1919002
start: 46
end: 51
orientation: inline

S-Tag
type: SequenceAnnotation
persistent identity: annotation2482485
display id: annotation2482485
version: 1
top level: BBa_K1919002
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1919002/annotation2482485/range2482485/1

https://synbiohub.org/public/igem/BBa_K1919002/annotation2482485/range2482485/1
type: Range
persistent identity: range2482485
display id: range2482485
version: 1
direction: direction/forward
top level: BBa_K1919002
start: 459
end: 503
orientation: inline

6xHis Tag
type: SequenceAnnotation
persistent identity: annotation2482483
display id: annotation2482483
version: 1
top level: BBa_K1919002
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1919002/annotation2482483/range2482483/1

https://synbiohub.org/public/igem/BBa_K1919002/annotation2482483/range2482483/1
type: Range
persistent identity: range2482483
display id: range2482483
version: 1
direction: direction/forward
top level: BBa_K1919002
start: 408
end: 425
orientation: inline

BSMT2
type: ComponentDefinition
persistent identity: BBa J45007
display id: BBa_J45007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: SAM:benzoic acid/salicylic acid carboxyl methyltransferase II; converts salicylic acid to methyl sal
creator: Boyuan Zhu
created: 2006-06-06T11:00:00Z
modified: 2015-08-31T04:08:49Z
discontinued: false
dominant: false
experience: experience/None
group u list: _84_
m user id: 0
owner id: 974
owning group id: 84
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Codes for BSMT enzyme to convert Benzoic acid/salicylic acid to methyl benzoate and methyl salicylate. This part is phBSMT2 from Petunia x hybrida.
mutable notes: Transport issues.
mutable provenance: GenBank Petunia x hybrida.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: BSMT2
sequence annotation: stop codon
sequence annotation: start codon
sequence: https://synbiohub.org/public/igem/BBa_J45007_sequence/1

BSMT2
type: SequenceAnnotation
persistent identity: annotation1880521
display id: annotation1880521
version: 1
top level: BSMT2
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J45007/annotation1880521/range1880521/1

https://synbiohub.org/public/igem/BBa_J45007/annotation1880521/range1880521/1
type: Range
persistent identity: range1880521
display id: range1880521
version: 1
direction: direction/forward
top level: BSMT2
start: 1
end: 1074
orientation: inline

stop codon
type: SequenceAnnotation
persistent identity: annotation1880524
display id: annotation1880524
version: 1
top level: BSMT2
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_J45007/annotation1880524/range1880524/1

https://synbiohub.org/public/igem/BBa_J45007/annotation1880524/range1880524/1
type: Range
persistent identity: range1880524
display id: range1880524
version: 1
direction: direction/forward
top level: BSMT2
start: 1071
end: 1074
orientation: inline

start codon
type: SequenceAnnotation
persistent identity: annotation1880523
display id: annotation1880523
version: 1
top level: BSMT2
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_J45007/annotation1880523/range1880523/1

https://synbiohub.org/public/igem/BBa_J45007/annotation1880523/range1880523/1
type: Range
persistent identity: range1880523
display id: range1880523
version: 1
direction: direction/forward
top level: BSMT2
start: 1
end: 3
orientation: inline

VanBP
type: ComponentDefinition
persistent identity: BBa J58105
display id: BBa_J58105
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Synthetic periplasmic binding protein that docks a vanillin molecule
creator: Valencia iGEM 2006
created: 2006-10-25T11:00:00Z
modified: 2015-08-31T03:14:48Z
discontinued: false
dominant: false
experience: experience/None
group u list: _83_
m user id: 0
owner id: 1100
owning group id: 83
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: none
mutable notes: none
mutable provenance: none
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_J58105_sequence/1

SWOG
type: ComponentDefinition
persistent identity: BBa J45150
display id: BBa_J45150
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Stationary phase dependent wintergreen odor generator
creator: MIT IGEM 2006
created: 2006-08-09T11:00:00Z
modified: 2015-08-31T04:08:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _84_
m user id: 0
owner id: 1147
owning group id: 84
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is the wintergreen generating device hooked up to a stationary phase promoter with RBS B0030.
mutable notes: Still in development as SPP is not confirmed to work.
mutable provenance: Biobricks of osmY promoter and intermediate 45099.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0012
component: BBa_J45993
component: BBa_B0010
component: BBa_J45004
component: BBa_B0030
sequence annotation: BBa_B0012
sequence annotation: BBa_B0030
sequence annotation: BBa_J45993
sequence annotation: BBa_B0010
sequence annotation: BBa_J45004
sequence: https://synbiohub.org/public/igem/BBa_J45150_sequence/1

BBa_B0012
type: Component
persistent identity: component1894795
display id: component1894795
version: 1
top level: SWOG
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_J45993
type: Component
persistent identity: component1894785
display id: component1894785
version: 1
top level: SWOG
access: public
definition: https://synbiohub.org/public/igem/BBa_J45993/1

BBa_B0010
type: Component
persistent identity: component1894793
display id: component1894793
version: 1
top level: SWOG
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_J45004
type: Component
persistent identity: component1894792
display id: component1894792
version: 1
top level: SWOG
access: public
definition: BSMT1

BBa_B0030
type: Component
persistent identity: component1894787
display id: component1894787
version: 1
top level: SWOG
access: public
definition: https://synbiohub.org/public/igem/BBa_B0030/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1894795
display id: annotation1894795
version: 1
top level: SWOG
location: https://synbiohub.org/public/igem/BBa_J45150/annotation1894795/range1894795/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_J45150/annotation1894795/range1894795/1
type: Range
persistent identity: range1894795
display id: range1894795
version: 1
direction: direction/forward
top level: SWOG
start: 1257
end: 1297
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation1894787
display id: annotation1894787
version: 1
top level: SWOG
location: https://synbiohub.org/public/igem/BBa_J45150/annotation1894787/range1894787/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_J45150/annotation1894787/range1894787/1
type: Range
persistent identity: range1894787
display id: range1894787
version: 1
direction: direction/forward
top level: SWOG
start: 66
end: 80
orientation: inline

BBa_J45993
type: SequenceAnnotation
persistent identity: annotation1894785
display id: annotation1894785
version: 1
top level: SWOG
location: https://synbiohub.org/public/igem/BBa_J45150/annotation1894785/range1894785/1
component: BBa_J45993

https://synbiohub.org/public/igem/BBa_J45150/annotation1894785/range1894785/1
type: Range
persistent identity: range1894785
display id: range1894785
version: 1
direction: direction/reverse
top level: SWOG
start: 1
end: 57
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1894793
display id: annotation1894793
version: 1
top level: SWOG
location: https://synbiohub.org/public/igem/BBa_J45150/annotation1894793/range1894793/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_J45150/annotation1894793/range1894793/1
type: Range
persistent identity: range1894793
display id: range1894793
version: 1
direction: direction/forward
top level: SWOG
start: 1169
end: 1248
orientation: inline

BBa_J45004
type: SequenceAnnotation
persistent identity: annotation1894792
display id: annotation1894792
version: 1
top level: SWOG
location: https://synbiohub.org/public/igem/BBa_J45150/annotation1894792/range1894792/1
component: BBa_J45004

https://synbiohub.org/public/igem/BBa_J45150/annotation1894792/range1894792/1
type: Range
persistent identity: range1894792
display id: range1894792
version: 1
direction: direction/reverse
top level: SWOG
start: 87
end: 1160
orientation: inline

THI3
type: ComponentDefinition
persistent identity: BBa J45009
display id: BBa_J45009
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: ketoisocaproate decarboxylase (THI3); converts alpha-ketoisocaproate to 3-methylbutanal
creator: Veena Venkatachalam
created: 2006-06-06T11:00:00Z
modified: 2015-08-31T04:08:49Z
discontinued: false
dominant: false
experience: experience/Fails
group u list: _84_
m user id: 0
owner id: 642
owning group id: 84
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: dfsd
mutable notes: dfd
mutable provenance: dfd
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: a->g to eliminate EcoRI, to do
sequence annotation: start codon
sequence annotation: t->c to eliminate SpeI, to do
sequence annotation: THI3
sequence annotation: stop codon
sequence annotation: t->c to eliminate EcoRI, to do
sequence: https://synbiohub.org/public/igem/BBa_J45009_sequence/1

a->g to eliminate EcoRI, to do
type: SequenceAnnotation
persistent identity: annotation1891630
display id: annotation1891630
version: 1
top level: THI3
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_J45009/annotation1891630/range1891630/1

https://synbiohub.org/public/igem/BBa_J45009/annotation1891630/range1891630/1
type: Range
persistent identity: range1891630
display id: range1891630
version: 1
direction: direction/forward
top level: THI3
start: 1605
end: 1605
orientation: inline

start codon
type: SequenceAnnotation
persistent identity: annotation1961303
display id: annotation1961303
version: 1
top level: THI3
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_J45009/annotation1961303/range1961303/1

https://synbiohub.org/public/igem/BBa_J45009/annotation1961303/range1961303/1
type: Range
persistent identity: range1961303
display id: range1961303
version: 1
direction: direction/forward
top level: THI3
start: 1
end: 3
orientation: inline

t->c to eliminate SpeI, to do
type: SequenceAnnotation
persistent identity: annotation1891632
display id: annotation1891632
version: 1
top level: THI3
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_J45009/annotation1891632/range1891632/1

https://synbiohub.org/public/igem/BBa_J45009/annotation1891632/range1891632/1
type: Range
persistent identity: range1891632
display id: range1891632
version: 1
direction: direction/forward
top level: THI3
start: 699
end: 699
orientation: inline

THI3
type: SequenceAnnotation
persistent identity: annotation1961298
display id: annotation1961298
version: 1
top level: THI3
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J45009/annotation1961298/range1961298/1

https://synbiohub.org/public/igem/BBa_J45009/annotation1961298/range1961298/1
type: Range
persistent identity: range1961298
display id: range1961298
version: 1
direction: direction/forward
top level: THI3
start: 1
end: 1830
orientation: inline

a->g to eliminate EcoRI, to do
type: SequenceAnnotation
persistent identity: annotation1891631
display id: annotation1891631
version: 1
top level: THI3
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_J45009/annotation1891631/range1891631/1

https://synbiohub.org/public/igem/BBa_J45009/annotation1891631/range1891631/1
type: Range
persistent identity: range1891631
display id: range1891631
version: 1
direction: direction/forward
top level: THI3
start: 1791
end: 1791
orientation: inline

stop codon
type: SequenceAnnotation
persistent identity: annotation1961304
display id: annotation1961304
version: 1
top level: THI3
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_J45009/annotation1961304/range1961304/1

https://synbiohub.org/public/igem/BBa_J45009/annotation1961304/range1961304/1
type: Range
persistent identity: range1961304
display id: range1961304
version: 1
direction: direction/forward
top level: THI3
start: 1828
end: 1830
orientation: inline

a->g to eliminate EcoRI, to do
type: SequenceAnnotation
persistent identity: annotation1891629
display id: annotation1891629
version: 1
top level: THI3
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_J45009/annotation1891629/range1891629/1

https://synbiohub.org/public/igem/BBa_J45009/annotation1891629/range1891629/1
type: Range
persistent identity: range1891629
display id: range1891629
version: 1
direction: direction/forward
top level: THI3
start: 906
end: 906
orientation: inline

t->c to eliminate EcoRI, to do
type: SequenceAnnotation
persistent identity: annotation1891628
display id: annotation1891628
version: 1
top level: THI3
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_J45009/annotation1891628/range1891628/1

https://synbiohub.org/public/igem/BBa_J45009/annotation1891628/range1891628/1
type: Range
persistent identity: range1891628
display id: range1891628
version: 1
direction: direction/forward
top level: THI3
start: 6
end: 6
orientation: inline

Banana
type: ComponentDefinition
persistent identity: BBa J45200
display id: BBa_J45200
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Banana odor generator
creator: Andr???? Green II
created: 2006-10-19T11:00:00Z
modified: 2015-08-31T04:08:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _84_
m user id: 0
owner id: 977
owning group id: 84
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: Protein generator converting PoPS to the protein ATF1. ATFI encodes the enzyme alcohol acetyltransferase I (AATase I), which produces acetate esters from acetyl CoA and alcohols.
mutable notes: This part is the ATF sequence BBa_45J006 with a single point mutation at base pair 1148 (T -> C). Despite the mutation, the enzyme is still functional (kinetic studies have not been done to determine realitive functionality).
mutable provenance: This part was cloned from S. cerevisiae.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0030
component: BBa_R0040
component: BBa_B0012
component: BBa_B0010
component: BBa_J45014
sequence annotation: BBa_B0010
sequence annotation: BBa_B0030
sequence annotation: BBa_B0012
sequence annotation: BBa_J45014
sequence annotation: BBa_R0040
sequence: https://synbiohub.org/public/igem/BBa_J45200_sequence/1

BBa_B0030
type: Component
persistent identity: component1960592
display id: component1960592
version: 1
top level: Banana
access: public
definition: https://synbiohub.org/public/igem/BBa_B0030/1

BBa_R0040
type: Component
persistent identity: component1960587
display id: component1960587
version: 1
top level: Banana
access: public
definition: https://synbiohub.org/public/igem/BBa_R0040/1

BBa_B0012
type: Component
persistent identity: component1960601
display id: component1960601
version: 1
top level: Banana
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0010
type: Component
persistent identity: component1960599
display id: component1960599
version: 1
top level: Banana
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_J45014
type: Component
persistent identity: component1960598
display id: component1960598
version: 1
top level: Banana
access: public
definition: ATF1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1960599
display id: annotation1960599
version: 1
top level: Banana
location: https://synbiohub.org/public/igem/BBa_J45200/annotation1960599/range1960599/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_J45200/annotation1960599/range1960599/1
type: Range
persistent identity: range1960599
display id: range1960599
version: 1
direction: direction/forward
top level: Banana
start: 1673
end: 1752
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation1960592
display id: annotation1960592
version: 1
top level: Banana
location: https://synbiohub.org/public/igem/BBa_J45200/annotation1960592/range1960592/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_J45200/annotation1960592/range1960592/1
type: Range
persistent identity: range1960592
display id: range1960592
version: 1
direction: direction/forward
top level: Banana
start: 63
end: 77
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1960601
display id: annotation1960601
version: 1
top level: Banana
location: https://synbiohub.org/public/igem/BBa_J45200/annotation1960601/range1960601/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_J45200/annotation1960601/range1960601/1
type: Range
persistent identity: range1960601
display id: range1960601
version: 1
direction: direction/forward
top level: Banana
start: 1761
end: 1801
orientation: inline

BBa_J45014
type: SequenceAnnotation
persistent identity: annotation1960598
display id: annotation1960598
version: 1
top level: Banana
location: https://synbiohub.org/public/igem/BBa_J45200/annotation1960598/range1960598/1
component: BBa_J45014

https://synbiohub.org/public/igem/BBa_J45200/annotation1960598/range1960598/1
type: Range
persistent identity: range1960598
display id: range1960598
version: 1
direction: direction/reverse
top level: Banana
start: 84
end: 1664
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1960587
display id: annotation1960587
version: 1
top level: Banana
location: https://synbiohub.org/public/igem/BBa_J45200/annotation1960587/range1960587/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_J45200/annotation1960587/range1960587/1
type: Range
persistent identity: range1960587
display id: range1960587
version: 1
direction: direction/forward
top level: Banana
start: 1
end: 54
orientation: inline

BBa_J45396
type: ComponentDefinition
persistent identity: BBa J45396
display id: BBa_J45396
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: BAT2 enzyme translational unit
creator: Andr?? Green II
created: 2006-10-27T11:00:00Z
modified: 2015-08-31T04:08:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _84_
m user id: 0
owner id: 977
owning group id: 84
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This part is an intermediate to the protein generator BBa_45400.
mutable notes: coming
mutable provenance: coming
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_B0030
component: BBa_J45008
sequence annotation: BBa_J45008
sequence annotation: BBa_B0030
sequence: https://synbiohub.org/public/igem/BBa_J45396_sequence/1

BBa_B0030
type: Component
persistent identity: component1907892
display id: component1907892
version: 1
top level: BBa_J45396
access: public
definition: https://synbiohub.org/public/igem/BBa_B0030/1

BBa_J45008
type: Component
persistent identity: component1907897
display id: component1907897
version: 1
top level: BBa_J45396
access: public
definition: BAT2

BBa_J45008
type: SequenceAnnotation
persistent identity: annotation1907897
display id: annotation1907897
version: 1
top level: BBa_J45396
location: https://synbiohub.org/public/igem/BBa_J45396/annotation1907897/range1907897/1
component: BBa_J45008

https://synbiohub.org/public/igem/BBa_J45396/annotation1907897/range1907897/1
type: Range
persistent identity: range1907897
display id: range1907897
version: 1
direction: direction/reverse
top level: BBa_J45396
start: 22
end: 1155
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation1907892
display id: annotation1907892
version: 1
top level: BBa_J45396
location: https://synbiohub.org/public/igem/BBa_J45396/annotation1907892/range1907892/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_J45396/annotation1907892/range1907892/1
type: Range
persistent identity: range1907892
display id: range1907892
version: 1
direction: direction/forward
top level: BBa_J45396
start: 1
end: 15
orientation: inline

ATF1
type: ComponentDefinition
persistent identity: BBa K1379008
display id: BBa_K1379008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Constitutive banana odor enzyme (ATF1) generator
creator: Nadia Benedicta Bongso
created: 2014-09-22T11:00:00Z
modified: 2015-05-08T01:10:12Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1756_
m user id: 0
owner id: 16893
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Cis-Repressor MCR
mutable notes: intermediate part
mutable provenance: Synthetic
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_J45219
component: BBa_J23102
sequence annotation: BBa_J45219
sequence annotation: BBa_J23102
sequence: https://synbiohub.org/public/igem/BBa_K1379008_sequence/1

BBa_J45219
type: Component
persistent identity: component2430245
display id: component2430245
version: 1
top level: ATF1
access: public
definition: BBa_J45219

BBa_J23102
type: Component
persistent identity: component2430230
display id: component2430230
version: 1
top level: ATF1
access: public
definition: https://synbiohub.org/public/igem/BBa_J23102/1

BBa_J45219
type: SequenceAnnotation
persistent identity: annotation2430245
display id: annotation2430245
version: 1
top level: ATF1
location: https://synbiohub.org/public/igem/BBa_K1379008/annotation2430245/range2430245/1
component: BBa_J45219

https://synbiohub.org/public/igem/BBa_K1379008/annotation2430245/range2430245/1
type: Range
persistent identity: range2430245
display id: range2430245
version: 1
direction: direction/reverse
top level: ATF1
start: 44
end: 1780
orientation: inline

BBa_J23102
type: SequenceAnnotation
persistent identity: annotation2430230
display id: annotation2430230
version: 1
top level: ATF1
location: https://synbiohub.org/public/igem/BBa_K1379008/annotation2430230/range2430230/1
component: BBa_J23102

https://synbiohub.org/public/igem/BBa_K1379008/annotation2430230/range2430230/1
type: Range
persistent identity: range2430230
display id: range2430230
version: 1
direction: direction/reverse
top level: ATF1
start: 1
end: 35
orientation: inline

rbs+COMT
type: ComponentDefinition
persistent identity: BBa I742109
display id: BBa_I742109
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: COMT gene with ribosome binding site
creator: sarah hollingshead
created: 2007-10-18T11:00:00Z
modified: 2015-08-31T04:08:02Z
discontinued: false
dominant: false
experience: experience/None
group u list: _123_
m user id: 0
owner id: 1964
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: COMT (caffeic acid-O-methyl transferase) with ribosome binding site. Enzyme methylates -OH on C4 of the caffeic acid aromatic ring to produce ferulic acid. COMT is the third gene in the vanillin biosynthesis pathway,
mutable notes: Used COMT cDNA as genomic DNA contains introns
mutable provenance: Genes and Enzymes Involved in Caffeic Acid Biosynthesis in the Actinomycete Saccharothrix esanaensis, Berner M, et al, Journal of Bacteriology 188(7): 2666, (2006) Gowri G, et al, Molecular cloning and expression of alfalfa S-adenosyl-L-methionine: caffeic acid 3-0-methyltransferase, a key enzyme of lignin biosynthesis, Plant Physiology 97(1):7 (1991) Downregulation of Caffeic Acid 3-O-Methyltransferase and Caffeoyl CoA 3-O-Methyltransferase in Transgenic Alfalfa Impacts on Lignin Structure and Implications for the Biosynthesis of G and S Lignin, Guo D, et al, Plant Cell. January; 13(1): 73 (2001)
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_I742107
component: BBa_J15001
sequence annotation: BBa_J15001
sequence annotation: BBa_I742107
sequence: https://synbiohub.org/public/igem/BBa_I742109_sequence/1

BBa_I742107
type: Component
persistent identity: component1949771
display id: component1949771
version: 1
top level: rbs+COMT
access: public
definition: COMT

BBa_J15001
type: Component
persistent identity: component1949770
display id: component1949770
version: 1
top level: rbs+COMT
access: public
definition: https://synbiohub.org/public/igem/BBa_J15001/1

BBa_J15001
type: SequenceAnnotation
persistent identity: annotation1949770
display id: annotation1949770
version: 1
top level: rbs+COMT
location: https://synbiohub.org/public/igem/BBa_I742109/annotation1949770/range1949770/1
component: BBa_J15001

https://synbiohub.org/public/igem/BBa_I742109/annotation1949770/range1949770/1
type: Range
persistent identity: range1949770
display id: range1949770
version: 1
direction: direction/reverse
top level: rbs+COMT
start: 1
end: 10
orientation: inline

BBa_I742107
type: SequenceAnnotation
persistent identity: annotation1949771
display id: annotation1949771
version: 1
top level: rbs+COMT
location: https://synbiohub.org/public/igem/BBa_I742109/annotation1949771/range1949771/1
component: BBa_I742107

https://synbiohub.org/public/igem/BBa_I742109/annotation1949771/range1949771/1
type: Range
persistent identity: range1949771
display id: range1949771
version: 1
direction: direction/reverse
top level: rbs+COMT
start: 17
end: 1117
orientation: inline