//function/degradation/cellulose
type: Collection
persistent identity: function degradation cellulose
display id: function_degradation_cellulose
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
owned by: james
owned by: myers
member: CelC
member: BBa_J15507
member: BBa_J15508
member: BBa_K633002
member: Cel5A
member: BBa_J15510
member: BBa_J15509
member: BBa_K118028
member: BBa_K633000
member: BBa_K118022
member: BBa_K118023
member: BBa_K633014

BBa_K633014
type: ComponentDefinition
persistent identity: BBa K633014
display id: BBa_K633014
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: AraC+Pbad promoter+RBS+signal peptide phoA+Cellulase+Linker+estA membrane protein
creator: Ricardo Camilo Chavez Martinez
created: 2011-09-26T11:00:00Z
modified: 2015-06-19T01:50:43Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _807_
m user id: 4206
owner id: 9146
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: The composite part includes a functional expression vector for autotransporter membrane protein estA, with a linker united to a cellulase, attached to a membrane signaling peptide. This was our final construct missing a terminator
mutable notes: asdasdadd
mutable provenance: asdasda
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K633001
component: BBa_K206000
component: BBa_I13458
component: BBa_K633002
sequence annotation: BBa_I13458
sequence annotation: BBa_K633002
sequence annotation: BBa_K633001
sequence annotation: BBa_K206000
sequence: https://synbiohub.org/public/igem/BBa_K633014_sequence/1

BBa_K633001
type: Component
persistent identity: component2159642
display id: component2159642
version: 1
top level: BBa_K633014
access: public
definition: https://synbiohub.org/public/igem/BBa_K633001/1

BBa_K206000
type: Component
persistent identity: component2159635
display id: component2159635
version: 1
top level: BBa_K633014
access: public
definition: https://synbiohub.org/public/igem/BBa_K206000/1

BBa_I13458
type: Component
persistent identity: component2159631
display id: component2159631
version: 1
top level: BBa_K633014
access: public
definition: https://synbiohub.org/public/igem/BBa_I13458/1

BBa_K633002
type: Component
persistent identity: component2159639
display id: component2159639
version: 1
top level: BBa_K633014
access: public
definition: BBa_K633002

BBa_I13458
type: SequenceAnnotation
persistent identity: annotation2159631
display id: annotation2159631
version: 1
top level: BBa_K633014
location: https://synbiohub.org/public/igem/BBa_K633014/annotation2159631/range2159631/1
component: BBa_I13458

https://synbiohub.org/public/igem/BBa_K633014/annotation2159631/range2159631/1
type: Range
persistent identity: range2159631
display id: range2159631
version: 1
direction: direction/reverse
top level: BBa_K633014
start: 1
end: 1114
orientation: inline

BBa_K633002
type: SequenceAnnotation
persistent identity: annotation2159639
display id: annotation2159639
version: 1
top level: BBa_K633014
location: https://synbiohub.org/public/igem/BBa_K633014/annotation2159639/range2159639/1
component: BBa_K633002

https://synbiohub.org/public/igem/BBa_K633014/annotation2159639/range2159639/1
type: Range
persistent identity: range2159639
display id: range2159639
version: 1
direction: direction/reverse
top level: BBa_K633014
start: 1261
end: 3173
orientation: inline

BBa_K633001
type: SequenceAnnotation
persistent identity: annotation2159642
display id: annotation2159642
version: 1
top level: BBa_K633014
location: https://synbiohub.org/public/igem/BBa_K633014/annotation2159642/range2159642/1
component: BBa_K633001

https://synbiohub.org/public/igem/BBa_K633014/annotation2159642/range2159642/1
type: Range
persistent identity: range2159642
display id: range2159642
version: 1
direction: direction/reverse
top level: BBa_K633014
start: 3182
end: 4057
orientation: inline

BBa_K206000
type: SequenceAnnotation
persistent identity: annotation2159635
display id: annotation2159635
version: 1
top level: BBa_K633014
location: https://synbiohub.org/public/igem/BBa_K633014/annotation2159635/range2159635/1
component: BBa_K206000

https://synbiohub.org/public/igem/BBa_K633014/annotation2159635/range2159635/1
type: Range
persistent identity: range2159635
display id: range2159635
version: 1
direction: direction/reverse
top level: BBa_K633014
start: 1123
end: 1252
orientation: inline

BBa_K633000
type: ComponentDefinition
persistent identity: BBa K633000
display id: BBa_K633000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CelD Mutated Cellulase
creator: Camilo Chavez & Thelma Bolon
created: 2011-09-25T11:00:00Z
modified: 2015-06-19T01:53:06Z
discontinued: false
dominant: false
experience: experience/None
group u list: _807_
m user id: 4206
owner id: 9146
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Cellulase with a mutation, giving it a smalle km
mutable notes: It also has a SacI site and a NheI site after prefix, and before suffix EcorI XbaI SacI Cellulase NheI SpeI PstI
mutable provenance: Source*
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Cellulase
sequence: https://synbiohub.org/public/igem/BBa_K633000_sequence/1

Cellulase
type: SequenceAnnotation
persistent identity: annotation2144959
display id: annotation2144959
version: 1
top level: BBa_K633000
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K633000/annotation2144959/range2144959/1

https://synbiohub.org/public/igem/BBa_K633000/annotation2144959/range2144959/1
type: Range
persistent identity: range2144959
display id: range2144959
version: 1
direction: direction/forward
top level: BBa_K633000
start: 1
end: 1836
orientation: inline

BBa_J15507
type: ComponentDefinition
persistent identity: BBa J15507
display id: BBa_J15507
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS+Cex (Exoglucanase)
creator: Kwabena O. Duedu
created: 2013-11-25T12:00:00Z
modified: 2015-08-31T04:08:33Z
discontinued: false
dominant: false
group u list: _163_
m user id: 0
owner id: 14847
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: RBS + Exoglucanase (cex, BBa_K118022) from the cellulolytic bacterium Cellulomonas fimi (accession M15824).
mutable notes: GC rich part. Extended denaturation stage in PCR with glycerol or commercial GC rich enhancers may be required
mutable provenance: Cellulomonas fimi exoglucanase cex (accession M15824) with RBS
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_J15001
component: BBa_K118022
sequence annotation: BBa_J15001
sequence annotation: BBa_K118022
sequence: https://synbiohub.org/public/igem/BBa_J15507_sequence/1

BBa_J15001
type: Component
persistent identity: component2371433
display id: component2371433
version: 1
top level: BBa_J15507
access: public
definition: https://synbiohub.org/public/igem/BBa_J15001/1

BBa_K118022
type: Component
persistent identity: component2371436
display id: component2371436
version: 1
top level: BBa_J15507
access: public
definition: BBa_K118022

BBa_J15001
type: SequenceAnnotation
persistent identity: annotation2371433
display id: annotation2371433
version: 1
top level: BBa_J15507
location: https://synbiohub.org/public/igem/BBa_J15507/annotation2371433/range2371433/1
component: BBa_J15001

https://synbiohub.org/public/igem/BBa_J15507/annotation2371433/range2371433/1
type: Range
persistent identity: range2371433
display id: range2371433
version: 1
direction: direction/reverse
top level: BBa_J15507
start: 1
end: 10
orientation: inline

BBa_K118022
type: SequenceAnnotation
persistent identity: annotation2371436
display id: annotation2371436
version: 1
top level: BBa_J15507
location: https://synbiohub.org/public/igem/BBa_J15507/annotation2371436/range2371436/1
component: BBa_K118022

https://synbiohub.org/public/igem/BBa_J15507/annotation2371436/range2371436/1
type: Range
persistent identity: range2371436
display id: range2371436
version: 1
direction: direction/reverse
top level: BBa_J15507
start: 11
end: 1471
orientation: inline

BBa_K118023
type: ComponentDefinition
persistent identity: BBa K118023
display id: BBa_K118023
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: cenA coding sequence encoding Cellulomonas fimi endoglucanase A
creator: Andrew Hall
created: 2008-10-06T11:00:00Z
modified: 2015-05-08T01:09:37Z
discontinued: false
dominant: false
experience: experience/None
group u list: _192_
m user id: 0
owner id: 3282
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: The cellulolytic bacterium ''Cellulomonas fimi'' uses 3 endoglucanase (including CenA) and an exoglucanase in the degradation of cellulose into cellobiose, before use B-glucosidase to catalyse the conversion of cellobiose to D-glucose.
mutable notes: No special considerations.
mutable provenance: Purified ''Cellulomonas fimi'' genomic DNA.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: cenA coding sequence
sequence: https://synbiohub.org/public/igem/BBa_K118023_sequence/1

cenA coding sequence
type: SequenceAnnotation
persistent identity: annotation1996991
display id: annotation1996991
version: 1
top level: BBa_K118023
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K118023/annotation1996991/range1996991/1

https://synbiohub.org/public/igem/BBa_K118023/annotation1996991/range1996991/1
type: Range
persistent identity: range1996991
display id: range1996991
version: 1
direction: direction/forward
top level: BBa_K118023
start: 1
end: 1350
orientation: inline

BBa_J15509
type: ComponentDefinition
persistent identity: BBa J15509
display id: BBa_J15509
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Plac+RBS+cex+RBS+cenA
creator: Kwabena O. Duedu, Chris French, Sahreena Lakhundi
created: 2013-11-25T12:00:00Z
modified: 2015-08-31T04:08:33Z
discontinued: false
dominant: false
experience: experience/None
group u list: _163_
m user id: 0
owner id: 14847
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Exo- and endo- glucanases from the cellulolytic bacterium Cellulomonas fimi under the E. coli lac promoter. Together with betaglucosidases, the bacterium degrades cellulose into D-glucose.
mutable notes: Cex is a GC rich part and may require extended denaturation in amplifying the gene.
mutable provenance: Cellulomonas fimi
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_J15507
component: BBa_J33207
component: BBa_J15508
sequence annotation: BBa_J33207
sequence annotation: BBa_J15507
sequence annotation: BBa_J15508
sequence: https://synbiohub.org/public/igem/BBa_J15509_sequence/1

BBa_J15507
type: Component
persistent identity: component2371456
display id: component2371456
version: 1
top level: BBa_J15509
access: public
definition: BBa_J15507

BBa_J33207
type: Component
persistent identity: component2371449
display id: component2371449
version: 1
top level: BBa_J15509
access: public
definition: https://synbiohub.org/public/igem/BBa_J33207/1

BBa_J15508
type: Component
persistent identity: component2371462
display id: component2371462
version: 1
top level: BBa_J15509
access: public
definition: BBa_J15508

BBa_J33207
type: SequenceAnnotation
persistent identity: annotation2371449
display id: annotation2371449
version: 1
top level: BBa_J15509
location: https://synbiohub.org/public/igem/BBa_J15509/annotation2371449/range2371449/1
component: BBa_J33207

https://synbiohub.org/public/igem/BBa_J15509/annotation2371449/range2371449/1
type: Range
persistent identity: range2371449
display id: range2371449
version: 1
direction: direction/reverse
top level: BBa_J15509
start: 1
end: 600
orientation: inline

BBa_J15507
type: SequenceAnnotation
persistent identity: annotation2371456
display id: annotation2371456
version: 1
top level: BBa_J15509
location: https://synbiohub.org/public/igem/BBa_J15509/annotation2371456/range2371456/1
component: BBa_J15507

https://synbiohub.org/public/igem/BBa_J15509/annotation2371456/range2371456/1
type: Range
persistent identity: range2371456
display id: range2371456
version: 1
direction: direction/reverse
top level: BBa_J15509
start: 601
end: 2071
orientation: inline

BBa_J15508
type: SequenceAnnotation
persistent identity: annotation2371462
display id: annotation2371462
version: 1
top level: BBa_J15509
location: https://synbiohub.org/public/igem/BBa_J15509/annotation2371462/range2371462/1
component: BBa_J15508

https://synbiohub.org/public/igem/BBa_J15509/annotation2371462/range2371462/1
type: Range
persistent identity: range2371462
display id: range2371462
version: 1
direction: direction/reverse
top level: BBa_J15509
start: 2072
end: 3434
orientation: inline

BBa_J15510
type: ComponentDefinition
persistent identity: BBa J15510
display id: BBa_J15510
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Pspac+RBS+cex+RBS+cenA
creator: Kwabena O. Duedu, Chris French, Sahreena Lakhundi
created: 2013-11-25T12:00:00Z
modified: 2015-08-31T04:08:33Z
discontinued: false
dominant: false
experience: experience/None
group u list: _163_
m user id: 0
owner id: 14847
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Exo- and endo- glucanases from the cellulolytic bacterium Cellulomonas fimi under the E. coli spac promoter (BBa_J15506). Together with betaglucosidases, the bacterium degrades cellulose into D-glucose.
mutable notes: Cex is a GC rich part and requires extended denaturation with glycerol or commercial GC enhancers
mutable provenance: Cellulomonas fimi
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_J15503
component: BBa_J15507
component: BBa_J15508
sequence annotation: BBa_J15503
sequence annotation: BBa_J15508
sequence annotation: BBa_J15507
sequence: https://synbiohub.org/public/igem/BBa_J15510_sequence/1

BBa_J15503
type: Component
persistent identity: component2371463
display id: component2371463
version: 1
top level: BBa_J15510
access: public
definition: https://synbiohub.org/public/igem/BBa_J15503/1

BBa_J15507
type: Component
persistent identity: component2371470
display id: component2371470
version: 1
top level: BBa_J15510
access: public
definition: BBa_J15507

BBa_J15508
type: Component
persistent identity: component2371476
display id: component2371476
version: 1
top level: BBa_J15510
access: public
definition: BBa_J15508

BBa_J15503
type: SequenceAnnotation
persistent identity: annotation2371463
display id: annotation2371463
version: 1
top level: BBa_J15510
location: https://synbiohub.org/public/igem/BBa_J15510/annotation2371463/range2371463/1
component: BBa_J15503

https://synbiohub.org/public/igem/BBa_J15510/annotation2371463/range2371463/1
type: Range
persistent identity: range2371463
display id: range2371463
version: 1
direction: direction/reverse
top level: BBa_J15510
start: 1
end: 284
orientation: inline

BBa_J15508
type: SequenceAnnotation
persistent identity: annotation2371476
display id: annotation2371476
version: 1
top level: BBa_J15510
location: https://synbiohub.org/public/igem/BBa_J15510/annotation2371476/range2371476/1
component: BBa_J15508

https://synbiohub.org/public/igem/BBa_J15510/annotation2371476/range2371476/1
type: Range
persistent identity: range2371476
display id: range2371476
version: 1
direction: direction/reverse
top level: BBa_J15510
start: 1756
end: 3118
orientation: inline

BBa_J15507
type: SequenceAnnotation
persistent identity: annotation2371470
display id: annotation2371470
version: 1
top level: BBa_J15510
location: https://synbiohub.org/public/igem/BBa_J15510/annotation2371470/range2371470/1
component: BBa_J15507

https://synbiohub.org/public/igem/BBa_J15510/annotation2371470/range2371470/1
type: Range
persistent identity: range2371470
display id: range2371470
version: 1
direction: direction/reverse
top level: BBa_J15510
start: 285
end: 1755
orientation: inline

BBa_K118022
type: ComponentDefinition
persistent identity: BBa K118022
display id: BBa_K118022
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: cex coding sequence encoding Cellulomonas fimi exoglucanase
creator: Andrew Hall
created: 2008-10-06T11:00:00Z
modified: 2015-05-08T01:09:37Z
discontinued: false
dominant: false
experience: experience/None
group u list: _192_
m user id: 0
owner id: 3282
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: The fungus ''Cellulomonas fimi'' uses an exoglucanase (from ''cex'') along with 3 endoglucanases in the degradation of cellulose into cellobiose, before use B-glucosidase to catalyse the conversion of cellobiose to D-glucose.
mutable notes: The original sequence had a PstI site for 781-786. We added the silent mutation CAG->CAA (Gln) by MABEL to remove this site.
mutable provenance: Purified ''Cellulomonas fimi'' genomic DNA.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: PstI site removed
sequence annotation: cex coding sequence
sequence: https://synbiohub.org/public/igem/BBa_K118022_sequence/1

PstI site removed
type: SequenceAnnotation
persistent identity: annotation1996994
display id: annotation1996994
version: 1
top level: BBa_K118022
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_K118022/annotation1996994/range1996994/1

https://synbiohub.org/public/igem/BBa_K118022/annotation1996994/range1996994/1
type: Range
persistent identity: range1996994
display id: range1996994
version: 1
direction: direction/forward
top level: BBa_K118022
start: 781
end: 786
orientation: inline

cex coding sequence
type: SequenceAnnotation
persistent identity: annotation1996993
display id: annotation1996993
version: 1
top level: BBa_K118022
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K118022/annotation1996993/range1996993/1

https://synbiohub.org/public/igem/BBa_K118022/annotation1996993/range1996993/1
type: Range
persistent identity: range1996993
display id: range1996993
version: 1
direction: direction/forward
top level: BBa_K118022
start: 1
end: 1458
orientation: inline

Cel5A
type: ComponentDefinition
persistent identity: BBa K805011
display id: BBa_K805011
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Cel5A, endoglucanases
creator: Liu Ping
created: 2012-09-19T11:00:00Z
modified: 2015-06-19T01:48:30Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1062_
m user id: 4206
owner id: 11895
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: test
mutable notes: test
mutable provenance: yes
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: start codon
sequence annotation: protein
sequence: https://synbiohub.org/public/igem/BBa_K805011_sequence/1

start codon
type: SequenceAnnotation
persistent identity: annotation2200607
display id: annotation2200607
version: 1
top level: Cel5A
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K805011/annotation2200607/range2200607/1

https://synbiohub.org/public/igem/BBa_K805011/annotation2200607/range2200607/1
type: Range
persistent identity: range2200607
display id: range2200607
version: 1
direction: direction/forward
top level: Cel5A
start: 1
end: 3
orientation: inline

protein
type: SequenceAnnotation
persistent identity: annotation2200608
display id: annotation2200608
version: 1
top level: Cel5A
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K805011/annotation2200608/range2200608/1

https://synbiohub.org/public/igem/BBa_K805011/annotation2200608/range2200608/1
type: Range
persistent identity: range2200608
display id: range2200608
version: 1
direction: direction/forward
top level: Cel5A
start: 4
end: 1233
orientation: inline

CelC
type: ComponentDefinition
persistent identity: BBa K1044000
display id: BBa_K1044000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Endoglucanase gene CelC from Agrobacterium tumefaciens C58.
creator: Koki Tsutsumi
created: 2013-09-04T11:00:00Z
modified: 2015-06-19T01:58:03Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1351_
m user id: 4206
owner id: 16207
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This is Endoglucanase gene (CelC) from the genome of Agrobacterium tumefaciens C58.
mutable notes: None
mutable provenance: The source ofEndoglucanase genes (CelC) is GenBank(NC_003063).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: cds
sequence: https://synbiohub.org/public/igem/BBa_K1044000_sequence/1

cds
type: SequenceAnnotation
persistent identity: annotation2334971
display id: annotation2334971
version: 1
top level: CelC
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1044000/annotation2334971/range2334971/1

https://synbiohub.org/public/igem/BBa_K1044000/annotation2334971/range2334971/1
type: Range
persistent identity: range2334971
display id: range2334971
version: 1
direction: direction/forward
top level: CelC
start: 1
end: 1056
orientation: inline

BBa_K633002
type: ComponentDefinition
persistent identity: BBa K633002
display id: BBa_K633002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS+signal peptide phoA+Cellulase
creator: Ricardo Camilo Chavez Martinez
created: 2011-09-26T11:00:00Z
modified: 2015-06-19T01:49:52Z
discontinued: false
dominant: false
group u list: _807_
m user id: 4206
owner id: 9146
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: We used Rybosome Binding Site BBa_B0034 designed by by Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr, evaluated by Warsaw's 2010 team. we added membrane protein signal peptide phoA and cellulase immediately next to it
mutable notes: Since this part is supposed to be attached to part BBa_K633001 in order to geerate part BBa_K633014, we had to begin with both the signal peptide and then the extracelular domain, which is located on the N terminal side of the polypeptide chain.
mutable provenance: Source
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: part BBa_B0034
sequence annotation: Cellulase
sequence annotation: Signal Peptide
sequence: https://synbiohub.org/public/igem/BBa_K633002_sequence/1

part BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2145137
display id: annotation2145137
version: 1
top level: BBa_K633002
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K633002/annotation2145137/range2145137/1

https://synbiohub.org/public/igem/BBa_K633002/annotation2145137/range2145137/1
type: Range
persistent identity: range2145137
display id: range2145137
version: 1
direction: direction/forward
top level: BBa_K633002
start: 1
end: 14
orientation: inline

Cellulase
type: SequenceAnnotation
persistent identity: annotation2145250
display id: annotation2145250
version: 1
top level: BBa_K633002
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K633002/annotation2145250/range2145250/1

https://synbiohub.org/public/igem/BBa_K633002/annotation2145250/range2145250/1
type: Range
persistent identity: range2145250
display id: range2145250
version: 1
direction: direction/forward
top level: BBa_K633002
start: 78
end: 1913
orientation: inline

Signal Peptide
type: SequenceAnnotation
persistent identity: annotation2145138
display id: annotation2145138
version: 1
top level: BBa_K633002
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K633002/annotation2145138/range2145138/1

https://synbiohub.org/public/igem/BBa_K633002/annotation2145138/range2145138/1
type: Range
persistent identity: range2145138
display id: range2145138
version: 1
direction: direction/forward
top level: BBa_K633002
start: 15
end: 77
orientation: inline

BBa_J15508
type: ComponentDefinition
persistent identity: BBa J15508
display id: BBa_J15508
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS+CenA (Endoglucanase)
creator: Kwabena O. Duedu
created: 2013-11-25T12:00:00Z
modified: 2015-08-31T04:08:33Z
discontinued: false
dominant: false
group u list: _163_
m user id: 0
owner id: 14847
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Endoglucanase A (CenA) from the cellulolytic bacterium Cellulomonas fimi (accession M15823) used in the degradation of cellulose into cellobiose, before using beta-glucosidase to catalyse the conversion of cellobiose to D-glucose.
mutable notes: No special conditions required
mutable provenance: Cellulomonas fimi (accession M15823)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_J15001
component: BBa_K118023
sequence annotation: BBa_K118023
sequence annotation: BBa_J15001
sequence: https://synbiohub.org/public/igem/BBa_J15508_sequence/1

BBa_J15001
type: Component
persistent identity: component2371439
display id: component2371439
version: 1
top level: BBa_J15508
access: public
definition: https://synbiohub.org/public/igem/BBa_J15001/1

BBa_K118023
type: Component
persistent identity: component2371441
display id: component2371441
version: 1
top level: BBa_J15508
access: public
definition: BBa_K118023

BBa_K118023
type: SequenceAnnotation
persistent identity: annotation2371441
display id: annotation2371441
version: 1
top level: BBa_J15508
location: https://synbiohub.org/public/igem/BBa_J15508/annotation2371441/range2371441/1
component: BBa_K118023

https://synbiohub.org/public/igem/BBa_J15508/annotation2371441/range2371441/1
type: Range
persistent identity: range2371441
display id: range2371441
version: 1
direction: direction/reverse
top level: BBa_J15508
start: 11
end: 1363
orientation: inline

BBa_J15001
type: SequenceAnnotation
persistent identity: annotation2371439
display id: annotation2371439
version: 1
top level: BBa_J15508
location: https://synbiohub.org/public/igem/BBa_J15508/annotation2371439/range2371439/1
component: BBa_J15001

https://synbiohub.org/public/igem/BBa_J15508/annotation2371439/range2371439/1
type: Range
persistent identity: range2371439
display id: range2371439
version: 1
direction: direction/reverse
top level: BBa_J15508
start: 1
end: 10
orientation: inline

BBa_K118028
type: ComponentDefinition
persistent identity: BBa K118028
display id: BBa_K118028
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: beta-glucosidase gene bglX (chu_2268) from Cytophaga hutchinsonii
creator: Chris French
created: 2008-10-22T11:00:00Z
modified: 2015-05-08T01:09:37Z
discontinued: false
dominant: false
experience: experience/None
group u list: _192_
m user id: 0
owner id: 837
owning group id: 163
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: This is a beta-glucosidase gene (bglX, locus chu_2268) from the genome sequence of the cellulose-degrading Gram negative bacterium Cytophaga hutchinsonii.
mutable notes: No special considerations.
mutable provenance: Genome sequence of the cellulose-degrading Gram negative bacterium Cytophaga hutchinsonii.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: bglX coding sequence
sequence: https://synbiohub.org/public/igem/BBa_K118028_sequence/1

bglX coding sequence
type: SequenceAnnotation
persistent identity: annotation1985223
display id: annotation1985223
version: 1
top level: BBa_K118028
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K118028/annotation1985223/range1985223/1

https://synbiohub.org/public/igem/BBa_K118028/annotation1985223/range1985223/1
type: Range
persistent identity: range1985223
display id: range1985223
version: 1
direction: direction/forward
top level: BBa_K118028
start: 1
end: 2277
orientation: inline