//function/degradation/bisphenol
type: Collection
persistent identity: function degradation bisphenol
display id: function_degradation_bisphenol
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
owned by: james
owned by: myers
member: BisdAB
member: BBa_K525582
member: BBa_K123005
member: BBa_K525001
member: BBa_K525560
member: BBa_K123007
member: BBa_K525007
member: BisdB
member: BBa_K525562
member: BBa_K123009
member: BBa_K525008
member: BBa_K525551
member: BBa_K123008
member: BisdA
member: BBa_K525512
member: BBa_K525002

BBa_K525007
type: ComponentDefinition
persistent identity: BBa K525007
display id: BBa_K525007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Fusion protein of S-Layer SgsE with T7/lac and BisdA and BisdB
creator: Anna Drong
created: 2011-09-11T11:00:00Z
modified: 2015-05-08T01:12:34Z
discontinued: false
dominant: false
experience: experience/None
group u list: _690_
m user id: 0
owner id: 8543
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: PT7 + RBS + SgsE || BisdA || BisdB
mutable notes: Assembled
mutable provenance: BBa_K525303, BBa_K123000, BBa_K123001
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: K525515
sequence annotation: TAA
sequence annotation: T7
sequence annotation: BisdA
sequence annotation: K525301
sequence annotation: AgeI
sequence annotation: BisdB
sequence annotation: SgsE domain
sequence annotation: lac operator
sequence annotation: Freiburg Scar
sequence annotation: RBS
sequence annotation: ATG
sequence annotation: B0034
sequence annotation: NgoMIV
sequence: https://synbiohub.org/public/igem/BBa_K525007_sequence/1

K525515
type: SequenceAnnotation
persistent identity: annotation2161171
display id: annotation2161171
version: 1
top level: BBa_K525007
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K525007/annotation2161171/range2161171/1

https://synbiohub.org/public/igem/BBa_K525007/annotation2161171/range2161171/1
type: Range
persistent identity: range2161171
display id: range2161171
version: 1
direction: direction/forward
top level: BBa_K525007
start: 2407
end: 3999
orientation: inline

TAA
type: SequenceAnnotation
persistent identity: annotation2161176
display id: annotation2161176
version: 1
top level: BBa_K525007
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K525007/annotation2161176/range2161176/1

https://synbiohub.org/public/igem/BBa_K525007/annotation2161176/range2161176/1
type: Range
persistent identity: range2161176
display id: range2161176
version: 1
direction: direction/forward
top level: BBa_K525007
start: 3997
end: 3999
orientation: inline

T7
type: SequenceAnnotation
persistent identity: annotation2161162
display id: annotation2161162
version: 1
top level: BBa_K525007
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K525007/annotation2161162/range2161162/1

https://synbiohub.org/public/igem/BBa_K525007/annotation2161162/range2161162/1
type: Range
persistent identity: range2161162
display id: range2161162
version: 1
direction: direction/forward
top level: BBa_K525007
start: 1
end: 20
orientation: inline

BisdA
type: SequenceAnnotation
persistent identity: annotation2161172
display id: annotation2161172
version: 1
top level: BBa_K525007
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K525007/annotation2161172/range2161172/1

https://synbiohub.org/public/igem/BBa_K525007/annotation2161172/range2161172/1
type: Range
persistent identity: range2161172
display id: range2161172
version: 1
direction: direction/forward
top level: BBa_K525007
start: 2407
end: 2718
orientation: inline

K525301
type: SequenceAnnotation
persistent identity: annotation2161168
display id: annotation2161168
version: 1
top level: BBa_K525007
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K525007/annotation2161168/range2161168/1

https://synbiohub.org/public/igem/BBa_K525007/annotation2161168/range2161168/1
type: Range
persistent identity: range2161168
display id: range2161168
version: 1
direction: direction/forward
top level: BBa_K525007
start: 73
end: 2400
orientation: inline

AgeI
type: SequenceAnnotation
persistent identity: annotation2161175
display id: annotation2161175
version: 1
top level: BBa_K525007
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K525007/annotation2161175/range2161175/1

https://synbiohub.org/public/igem/BBa_K525007/annotation2161175/range2161175/1
type: Range
persistent identity: range2161175
display id: range2161175
version: 1
direction: direction/forward
top level: BBa_K525007
start: 3991
end: 3996
orientation: inline

BisdB
type: SequenceAnnotation
persistent identity: annotation2161174
display id: annotation2161174
version: 1
top level: BBa_K525007
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K525007/annotation2161174/range2161174/1

https://synbiohub.org/public/igem/BBa_K525007/annotation2161174/range2161174/1
type: Range
persistent identity: range2161174
display id: range2161174
version: 1
direction: direction/forward
top level: BBa_K525007
start: 2725
end: 3999
orientation: inline

SgsE domain
type: SequenceAnnotation
persistent identity: annotation2161167
display id: annotation2161167
version: 1
top level: BBa_K525007
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K525007/annotation2161167/range2161167/1

https://synbiohub.org/public/igem/BBa_K525007/annotation2161167/range2161167/1
type: Range
persistent identity: range2161167
display id: range2161167
version: 1
direction: direction/forward
top level: BBa_K525007
start: 73
end: 2400
orientation: inline

lac operator
type: SequenceAnnotation
persistent identity: annotation2161163
display id: annotation2161163
version: 1
top level: BBa_K525007
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K525007/annotation2161163/range2161163/1

https://synbiohub.org/public/igem/BBa_K525007/annotation2161163/range2161163/1
type: Range
persistent identity: range2161163
display id: range2161163
version: 1
direction: direction/forward
top level: BBa_K525007
start: 20
end: 44
orientation: inline

Freiburg Scar
type: SequenceAnnotation
persistent identity: annotation2161173
display id: annotation2161173
version: 1
top level: BBa_K525007
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K525007/annotation2161173/range2161173/1

https://synbiohub.org/public/igem/BBa_K525007/annotation2161173/range2161173/1
type: Range
persistent identity: range2161173
display id: range2161173
version: 1
direction: direction/forward
top level: BBa_K525007
start: 2719
end: 2724
orientation: inline

Freiburg Scar
type: SequenceAnnotation
persistent identity: annotation2161170
display id: annotation2161170
version: 1
top level: BBa_K525007
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K525007/annotation2161170/range2161170/1

https://synbiohub.org/public/igem/BBa_K525007/annotation2161170/range2161170/1
type: Range
persistent identity: range2161170
display id: range2161170
version: 1
direction: direction/forward
top level: BBa_K525007
start: 2401
end: 2406
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2161165
display id: annotation2161165
version: 1
top level: BBa_K525007
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K525007/annotation2161165/range2161165/1

https://synbiohub.org/public/igem/BBa_K525007/annotation2161165/range2161165/1
type: Range
persistent identity: range2161165
display id: range2161165
version: 1
direction: direction/forward
top level: BBa_K525007
start: 56
end: 59
orientation: inline

ATG
type: SequenceAnnotation
persistent identity: annotation2161166
display id: annotation2161166
version: 1
top level: BBa_K525007
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K525007/annotation2161166/range2161166/1

https://synbiohub.org/public/igem/BBa_K525007/annotation2161166/range2161166/1
type: Range
persistent identity: range2161166
display id: range2161166
version: 1
direction: direction/forward
top level: BBa_K525007
start: 73
end: 75
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation2161164
display id: annotation2161164
version: 1
top level: BBa_K525007
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K525007/annotation2161164/range2161164/1

https://synbiohub.org/public/igem/BBa_K525007/annotation2161164/range2161164/1
type: Range
persistent identity: range2161164
display id: range2161164
version: 1
direction: direction/forward
top level: BBa_K525007
start: 53
end: 64
orientation: inline

NgoMIV
type: SequenceAnnotation
persistent identity: annotation2161169
display id: annotation2161169
version: 1
top level: BBa_K525007
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K525007/annotation2161169/range2161169/1

https://synbiohub.org/public/igem/BBa_K525007/annotation2161169/range2161169/1
type: Range
persistent identity: range2161169
display id: range2161169
version: 1
direction: direction/forward
top level: BBa_K525007
start: 76
end: 82
orientation: inline

BBa_K525008
type: ComponentDefinition
persistent identity: BBa K525008
display id: BBa_K525008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Fusion protein of S-Layer SbpA with T7/lac and BisdA and BisdB
creator: Anna Drong
created: 2011-09-11T11:00:00Z
modified: 2015-05-08T01:12:34Z
discontinued: false
dominant: false
experience: experience/None
group u list: _690_
m user id: 0
owner id: 8543
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: PT7 + RBS + SbpA || BisdA || BisdB
mutable notes: Assembled
mutable provenance: BBa_K525403 , BBa_K123000 , BBa_K123001
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: TAA
sequence annotation: ATG
sequence annotation: lac operator
sequence annotation: NgoMIV
sequence annotation: BisdB
sequence annotation: Freiburg Scar
sequence annotation: SbpA domain
sequence annotation: K525515
sequence annotation: BisdA
sequence annotation: K525401
sequence annotation: RBS
sequence annotation: AgeI
sequence annotation: B0034
sequence annotation: T7
sequence: https://synbiohub.org/public/igem/BBa_K525008_sequence/1

TAA
type: SequenceAnnotation
persistent identity: annotation2161206
display id: annotation2161206
version: 1
top level: BBa_K525008
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K525008/annotation2161206/range2161206/1

https://synbiohub.org/public/igem/BBa_K525008/annotation2161206/range2161206/1
type: Range
persistent identity: range2161206
display id: range2161206
version: 1
direction: direction/forward
top level: BBa_K525008
start: 4789
end: 4791
orientation: inline

ATG
type: SequenceAnnotation
persistent identity: annotation2161199
display id: annotation2161199
version: 1
top level: BBa_K525008
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K525008/annotation2161199/range2161199/1

https://synbiohub.org/public/igem/BBa_K525008/annotation2161199/range2161199/1
type: Range
persistent identity: range2161199
display id: range2161199
version: 1
direction: direction/forward
top level: BBa_K525008
start: 73
end: 75
orientation: inline

lac operator
type: SequenceAnnotation
persistent identity: annotation2161195
display id: annotation2161195
version: 1
top level: BBa_K525008
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K525008/annotation2161195/range2161195/1

https://synbiohub.org/public/igem/BBa_K525008/annotation2161195/range2161195/1
type: Range
persistent identity: range2161195
display id: range2161195
version: 1
direction: direction/forward
top level: BBa_K525008
start: 20
end: 44
orientation: inline

NgoMIV
type: SequenceAnnotation
persistent identity: annotation2161201
display id: annotation2161201
version: 1
top level: BBa_K525008
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K525008/annotation2161201/range2161201/1

https://synbiohub.org/public/igem/BBa_K525008/annotation2161201/range2161201/1
type: Range
persistent identity: range2161201
display id: range2161201
version: 1
direction: direction/forward
top level: BBa_K525008
start: 76
end: 82
orientation: inline

BisdB
type: SequenceAnnotation
persistent identity: annotation2161205
display id: annotation2161205
version: 1
top level: BBa_K525008
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K525008/annotation2161205/range2161205/1

https://synbiohub.org/public/igem/BBa_K525008/annotation2161205/range2161205/1
type: Range
persistent identity: range2161205
display id: range2161205
version: 1
direction: direction/forward
top level: BBa_K525008
start: 3517
end: 4791
orientation: inline

Freiburg Scar
type: SequenceAnnotation
persistent identity: annotation2161202
display id: annotation2161202
version: 1
top level: BBa_K525008
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K525008/annotation2161202/range2161202/1

https://synbiohub.org/public/igem/BBa_K525008/annotation2161202/range2161202/1
type: Range
persistent identity: range2161202
display id: range2161202
version: 1
direction: direction/forward
top level: BBa_K525008
start: 3193
end: 3198
orientation: inline

SbpA domain
type: SequenceAnnotation
persistent identity: annotation2161200
display id: annotation2161200
version: 1
top level: BBa_K525008
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K525008/annotation2161200/range2161200/1

https://synbiohub.org/public/igem/BBa_K525008/annotation2161200/range2161200/1
type: Range
persistent identity: range2161200
display id: range2161200
version: 1
direction: direction/forward
top level: BBa_K525008
start: 73
end: 3192
orientation: inline

K525515
type: SequenceAnnotation
persistent identity: annotation2161207
display id: annotation2161207
version: 1
top level: BBa_K525008
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K525008/annotation2161207/range2161207/1

https://synbiohub.org/public/igem/BBa_K525008/annotation2161207/range2161207/1
type: Range
persistent identity: range2161207
display id: range2161207
version: 1
direction: direction/forward
top level: BBa_K525008
start: 3199
end: 4791
orientation: inline

BisdA
type: SequenceAnnotation
persistent identity: annotation2161203
display id: annotation2161203
version: 1
top level: BBa_K525008
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K525008/annotation2161203/range2161203/1

https://synbiohub.org/public/igem/BBa_K525008/annotation2161203/range2161203/1
type: Range
persistent identity: range2161203
display id: range2161203
version: 1
direction: direction/forward
top level: BBa_K525008
start: 3199
end: 3510
orientation: inline

K525401
type: SequenceAnnotation
persistent identity: annotation2161198
display id: annotation2161198
version: 1
top level: BBa_K525008
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K525008/annotation2161198/range2161198/1

https://synbiohub.org/public/igem/BBa_K525008/annotation2161198/range2161198/1
type: Range
persistent identity: range2161198
display id: range2161198
version: 1
direction: direction/forward
top level: BBa_K525008
start: 73
end: 3192
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2161197
display id: annotation2161197
version: 1
top level: BBa_K525008
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K525008/annotation2161197/range2161197/1

https://synbiohub.org/public/igem/BBa_K525008/annotation2161197/range2161197/1
type: Range
persistent identity: range2161197
display id: range2161197
version: 1
direction: direction/forward
top level: BBa_K525008
start: 56
end: 59
orientation: inline

Freiburg Scar
type: SequenceAnnotation
persistent identity: annotation2161204
display id: annotation2161204
version: 1
top level: BBa_K525008
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K525008/annotation2161204/range2161204/1

https://synbiohub.org/public/igem/BBa_K525008/annotation2161204/range2161204/1
type: Range
persistent identity: range2161204
display id: range2161204
version: 1
direction: direction/forward
top level: BBa_K525008
start: 3511
end: 3516
orientation: inline

AgeI
type: SequenceAnnotation
persistent identity: annotation2161208
display id: annotation2161208
version: 1
top level: BBa_K525008
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K525008/annotation2161208/range2161208/1

https://synbiohub.org/public/igem/BBa_K525008/annotation2161208/range2161208/1
type: Range
persistent identity: range2161208
display id: range2161208
version: 1
direction: direction/forward
top level: BBa_K525008
start: 4783
end: 4788
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation2161196
display id: annotation2161196
version: 1
top level: BBa_K525008
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K525008/annotation2161196/range2161196/1

https://synbiohub.org/public/igem/BBa_K525008/annotation2161196/range2161196/1
type: Range
persistent identity: range2161196
display id: range2161196
version: 1
direction: direction/forward
top level: BBa_K525008
start: 53
end: 64
orientation: inline

T7
type: SequenceAnnotation
persistent identity: annotation2161194
display id: annotation2161194
version: 1
top level: BBa_K525008
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K525008/annotation2161194/range2161194/1

https://synbiohub.org/public/igem/BBa_K525008/annotation2161194/range2161194/1
type: Range
persistent identity: range2161194
display id: range2161194
version: 1
direction: direction/forward
top level: BBa_K525008
start: 1
end: 20
orientation: inline

BisdAB
type: ComponentDefinition
persistent identity: BBa K525517
display id: BBa_K525517
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Fusion protein of BisdA and BisdB behind constitutive promoter
creator: Jonas Aretz
created: 2011-09-13T11:00:00Z
modified: 2015-05-08T01:12:34Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _690_
m user id: 0
owner id: 6670
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Fusion protein of BisdA and BisdB behind constitutive promoter
mutable notes: Fusion protein of BisdA and BisdB behind constitutive promoter
mutable provenance: Assembled
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_K525515
component: BBa_J23110
component: BBa_B0034
sequence annotation: BBa_B0034
sequence annotation: BBa_J23110
sequence annotation: BBa_K525515
sequence: https://synbiohub.org/public/igem/BBa_K525517_sequence/1

BBa_K525515
type: Component
persistent identity: component2128151
display id: component2128151
version: 1
top level: BisdAB
access: public
definition: BisdAB

BBa_J23110
type: Component
persistent identity: component2128146
display id: component2128146
version: 1
top level: BisdAB
access: public
definition: https://synbiohub.org/public/igem/BBa_J23110/1

BBa_B0034
type: Component
persistent identity: component2128148
display id: component2128148
version: 1
top level: BisdAB
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2128148
display id: annotation2128148
version: 1
top level: BisdAB
location: https://synbiohub.org/public/igem/BBa_K525517/annotation2128148/range2128148/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K525517/annotation2128148/range2128148/1
type: Range
persistent identity: range2128148
display id: range2128148
version: 1
direction: direction/reverse
top level: BisdAB
start: 44
end: 55
orientation: inline

BBa_J23110
type: SequenceAnnotation
persistent identity: annotation2128146
display id: annotation2128146
version: 1
top level: BisdAB
location: https://synbiohub.org/public/igem/BBa_K525517/annotation2128146/range2128146/1
component: BBa_J23110

https://synbiohub.org/public/igem/BBa_K525517/annotation2128146/range2128146/1
type: Range
persistent identity: range2128146
display id: range2128146
version: 1
direction: direction/reverse
top level: BisdAB
start: 1
end: 35
orientation: inline

BBa_K525515
type: SequenceAnnotation
persistent identity: annotation2128151
display id: annotation2128151
version: 1
top level: BisdAB
location: https://synbiohub.org/public/igem/BBa_K525517/annotation2128151/range2128151/1
component: BBa_K525515

https://synbiohub.org/public/igem/BBa_K525517/annotation2128151/range2128151/1
type: Range
persistent identity: range2128151
display id: range2128151
version: 1
direction: direction/reverse
top level: BisdAB
start: 62
end: 1663
orientation: inline

BBa_K525002
type: ComponentDefinition
persistent identity: BBa K525002
display id: BBa_K525002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Fusion protein of SbpA, NADP+ Oxidoreductase, BisdA and BisdB with PT7/lac
creator: Anna Drong
created: 2011-09-10T11:00:00Z
modified: 2015-05-08T01:12:34Z
discontinued: false
dominant: false
experience: experience/None
group u list: _690_
m user id: 0
owner id: 8543
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: PT7 + RBS + SbpA || Red || BisdA || BisdB
mutable notes: Assembled
mutable provenance: BBa_K525403; BBa_K525499; BBa_K123000 ; BBa_K123001 ; RCU 25
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: BisdB
sequence annotation: lac operator
sequence annotation: BisdA
sequence annotation: K525401
sequence annotation: SbpA domain
sequence annotation: RBS
sequence annotation: NgoMIV
sequence annotation: Freiburg sacr
sequence annotation: Reductase
sequence annotation: T7 promoter
sequence annotation: K525560
sequence annotation: Freiburg scar
sequence annotation: TAA
sequence annotation: B0034
sequence annotation: ATG
sequence annotation: AgeI
sequence: https://synbiohub.org/public/igem/BBa_K525002_sequence/1

BisdB
type: SequenceAnnotation
persistent identity: annotation2160933
display id: annotation2160933
version: 1
top level: BBa_K525002
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K525002/annotation2160933/range2160933/1

https://synbiohub.org/public/igem/BBa_K525002/annotation2160933/range2160933/1
type: Range
persistent identity: range2160933
display id: range2160933
version: 1
direction: direction/forward
top level: BBa_K525002
start: 4263
end: 5538
orientation: inline

lac operator
type: SequenceAnnotation
persistent identity: annotation2160922
display id: annotation2160922
version: 1
top level: BBa_K525002
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K525002/annotation2160922/range2160922/1

https://synbiohub.org/public/igem/BBa_K525002/annotation2160922/range2160922/1
type: Range
persistent identity: range2160922
display id: range2160922
version: 1
direction: direction/forward
top level: BBa_K525002
start: 20
end: 40
orientation: inline

BisdA
type: SequenceAnnotation
persistent identity: annotation2160931
display id: annotation2160931
version: 1
top level: BBa_K525002
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K525002/annotation2160931/range2160931/1

https://synbiohub.org/public/igem/BBa_K525002/annotation2160931/range2160931/1
type: Range
persistent identity: range2160931
display id: range2160931
version: 1
direction: direction/forward
top level: BBa_K525002
start: 3946
end: 4258
orientation: inline

K525401
type: SequenceAnnotation
persistent identity: annotation2160921
display id: annotation2160921
version: 1
top level: BBa_K525002
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K525002/annotation2160921/range2160921/1

https://synbiohub.org/public/igem/BBa_K525002/annotation2160921/range2160921/1
type: Range
persistent identity: range2160921
display id: range2160921
version: 1
direction: direction/forward
top level: BBa_K525002
start: 73
end: 3192
orientation: inline

SbpA domain
type: SequenceAnnotation
persistent identity: annotation2160925
display id: annotation2160925
version: 1
top level: BBa_K525002
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K525002/annotation2160925/range2160925/1

https://synbiohub.org/public/igem/BBa_K525002/annotation2160925/range2160925/1
type: Range
persistent identity: range2160925
display id: range2160925
version: 1
direction: direction/forward
top level: BBa_K525002
start: 73
end: 3192
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2160923
display id: annotation2160923
version: 1
top level: BBa_K525002
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K525002/annotation2160923/range2160923/1

https://synbiohub.org/public/igem/BBa_K525002/annotation2160923/range2160923/1
type: Range
persistent identity: range2160923
display id: range2160923
version: 1
direction: direction/forward
top level: BBa_K525002
start: 56
end: 59
orientation: inline

NgoMIV
type: SequenceAnnotation
persistent identity: annotation2160926
display id: annotation2160926
version: 1
top level: BBa_K525002
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K525002/annotation2160926/range2160926/1

https://synbiohub.org/public/igem/BBa_K525002/annotation2160926/range2160926/1
type: Range
persistent identity: range2160926
display id: range2160926
version: 1
direction: direction/forward
top level: BBa_K525002
start: 76
end: 81
orientation: inline

Freiburg sacr
type: SequenceAnnotation
persistent identity: annotation2160932
display id: annotation2160932
version: 1
top level: BBa_K525002
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K525002/annotation2160932/range2160932/1

https://synbiohub.org/public/igem/BBa_K525002/annotation2160932/range2160932/1
type: Range
persistent identity: range2160932
display id: range2160932
version: 1
direction: direction/forward
top level: BBa_K525002
start: 4258
end: 4262
orientation: inline

Reductase
type: SequenceAnnotation
persistent identity: annotation2160928
display id: annotation2160928
version: 1
top level: BBa_K525002
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K525002/annotation2160928/range2160928/1

https://synbiohub.org/public/igem/BBa_K525002/annotation2160928/range2160928/1
type: Range
persistent identity: range2160928
display id: range2160928
version: 1
direction: direction/forward
top level: BBa_K525002
start: 3199
end: 3939
orientation: inline

T7 promoter
type: SequenceAnnotation
persistent identity: annotation2160920
display id: annotation2160920
version: 1
top level: BBa_K525002
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K525002/annotation2160920/range2160920/1

https://synbiohub.org/public/igem/BBa_K525002/annotation2160920/range2160920/1
type: Range
persistent identity: range2160920
display id: range2160920
version: 1
direction: direction/forward
top level: BBa_K525002
start: 1
end: 20
orientation: inline

K525560
type: SequenceAnnotation
persistent identity: annotation2160929
display id: annotation2160929
version: 1
top level: BBa_K525002
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K525002/annotation2160929/range2160929/1

https://synbiohub.org/public/igem/BBa_K525002/annotation2160929/range2160929/1
type: Range
persistent identity: range2160929
display id: range2160929
version: 1
direction: direction/forward
top level: BBa_K525002
start: 3199
end: 5538
orientation: inline

Freiburg scar
type: SequenceAnnotation
persistent identity: annotation2160927
display id: annotation2160927
version: 1
top level: BBa_K525002
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K525002/annotation2160927/range2160927/1

https://synbiohub.org/public/igem/BBa_K525002/annotation2160927/range2160927/1
type: Range
persistent identity: range2160927
display id: range2160927
version: 1
direction: direction/forward
top level: BBa_K525002
start: 3193
end: 3198
orientation: inline

TAA
type: SequenceAnnotation
persistent identity: annotation2160935
display id: annotation2160935
version: 1
top level: BBa_K525002
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K525002/annotation2160935/range2160935/1

https://synbiohub.org/public/igem/BBa_K525002/annotation2160935/range2160935/1
type: Range
persistent identity: range2160935
display id: range2160935
version: 1
direction: direction/forward
top level: BBa_K525002
start: 5536
end: 5538
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation2161005
display id: annotation2161005
version: 1
top level: BBa_K525002
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K525002/annotation2161005/range2161005/1

https://synbiohub.org/public/igem/BBa_K525002/annotation2161005/range2161005/1
type: Range
persistent identity: range2161005
display id: range2161005
version: 1
direction: direction/forward
top level: BBa_K525002
start: 53
end: 64
orientation: inline

ATG
type: SequenceAnnotation
persistent identity: annotation2160924
display id: annotation2160924
version: 1
top level: BBa_K525002
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K525002/annotation2160924/range2160924/1

https://synbiohub.org/public/igem/BBa_K525002/annotation2160924/range2160924/1
type: Range
persistent identity: range2160924
display id: range2160924
version: 1
direction: direction/forward
top level: BBa_K525002
start: 73
end: 75
orientation: inline

AgeI
type: SequenceAnnotation
persistent identity: annotation2160934
display id: annotation2160934
version: 1
top level: BBa_K525002
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K525002/annotation2160934/range2160934/1

https://synbiohub.org/public/igem/BBa_K525002/annotation2160934/range2160934/1
type: Range
persistent identity: range2160934
display id: range2160934
version: 1
direction: direction/forward
top level: BBa_K525002
start: 5530
end: 5535
orientation: inline

Freiburg scar
type: SequenceAnnotation
persistent identity: annotation2160930
display id: annotation2160930
version: 1
top level: BBa_K525002
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K525002/annotation2160930/range2160930/1

https://synbiohub.org/public/igem/BBa_K525002/annotation2160930/range2160930/1
type: Range
persistent identity: range2160930
display id: range2160930
version: 1
direction: direction/forward
top level: BBa_K525002
start: 3940
end: 3945
orientation: inline

BBa_K123007
type: ComponentDefinition
persistent identity: BBa K123007
display id: BBa_K123007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pTetBisdB
creator: Jason Gardiner
created: 2008-10-27T12:00:00Z
modified: 2015-05-08T01:09:44Z
discontinued: false
dominant: false
group u list: _241_
m user id: 0
owner id: 2742
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part has been designed to allow the presence/absence of TetR to control the production of BisdB
mutable notes: N/A
mutable provenance: Bisphenolics
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_R0040
component: BBa_K123001
sequence annotation: BBa_R0040
sequence annotation: BBa_K123001
sequence: https://synbiohub.org/public/igem/BBa_K123007_sequence/1

BBa_R0040
type: Component
persistent identity: component1992750
display id: component1992750
version: 1
top level: BBa_K123007
access: public
definition: https://synbiohub.org/public/igem/BBa_R0040/1

BBa_K123001
type: Component
persistent identity: component1992755
display id: component1992755
version: 1
top level: BBa_K123007
access: public
definition: BisdB

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1992750
display id: annotation1992750
version: 1
top level: BBa_K123007
location: https://synbiohub.org/public/igem/BBa_K123007/annotation1992750/range1992750/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_K123007/annotation1992750/range1992750/1
type: Range
persistent identity: range1992750
display id: range1992750
version: 1
direction: direction/forward
top level: BBa_K123007
start: 1
end: 54
orientation: inline

BBa_K123001
type: SequenceAnnotation
persistent identity: annotation1992755
display id: annotation1992755
version: 1
top level: BBa_K123007
location: https://synbiohub.org/public/igem/BBa_K123007/annotation1992755/range1992755/1
component: BBa_K123001

https://synbiohub.org/public/igem/BBa_K123007/annotation1992755/range1992755/1
type: Range
persistent identity: range1992755
display id: range1992755
version: 1
direction: direction/reverse
top level: BBa_K123007
start: 61
end: 1344
orientation: inline

BBa_K525562
type: ComponentDefinition
persistent identity: BBa K525562
display id: BBa_K525562
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Fusion protein of NADP+ Oxidoreductase and BisdA and BisdB with middle strong promoter,RBS
creator: Anna Drong
created: 2011-10-27T11:00:00Z
modified: 2015-05-08T01:12:34Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _690_
m user id: 0
owner id: 8543
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Fusion protein of ferredoxin-NADP+ oxidoreductase, BisdA and BisdB; RFC 25 (Freiburg BioBrick assembly standard) for characterization of intra- and extracellular BPA degradation.
mutable notes: Assembled
mutable provenance: K525499; K12300; K123001
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: NgoMIV
sequence annotation: Freiburg Scar
sequence annotation: ATG
sequence annotation: K123000
sequence annotation: B0034
sequence annotation: AgeI
sequence annotation: BisdB
sequence annotation: K123001
sequence annotation: Reductase
sequence annotation: TAA
sequence annotation: K525499
sequence annotation: BisdA
sequence annotation: RBS
sequence annotation: promoter
sequence: https://synbiohub.org/public/igem/BBa_K525562_sequence/1

NgoMIV
type: SequenceAnnotation
persistent identity: annotation2160995
display id: annotation2160995
version: 1
top level: BBa_K525562
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K525562/annotation2160995/range2160995/1

https://synbiohub.org/public/igem/BBa_K525562/annotation2160995/range2160995/1
type: Range
persistent identity: range2160995
display id: range2160995
version: 1
direction: direction/forward
top level: BBa_K525562
start: 65
end: 70
orientation: inline

Freiburg Scar
type: SequenceAnnotation
persistent identity: annotation2161138
display id: annotation2161138
version: 1
top level: BBa_K525562
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K525562/annotation2161138/range2161138/1

https://synbiohub.org/public/igem/BBa_K525562/annotation2161138/range2161138/1
type: Range
persistent identity: range2161138
display id: range2161138
version: 1
direction: direction/forward
top level: BBa_K525562
start: 1129
end: 1134
orientation: inline

Freiburg Scar
type: SequenceAnnotation
persistent identity: annotation2161292
display id: annotation2161292
version: 1
top level: BBa_K525562
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K525562/annotation2161292/range2161292/1

https://synbiohub.org/public/igem/BBa_K525562/annotation2161292/range2161292/1
type: Range
persistent identity: range2161292
display id: range2161292
version: 1
direction: direction/forward
top level: BBa_K525562
start: 812
end: 817
orientation: inline

ATG
type: SequenceAnnotation
persistent identity: annotation2160996
display id: annotation2160996
version: 1
top level: BBa_K525562
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K525562/annotation2160996/range2160996/1

https://synbiohub.org/public/igem/BBa_K525562/annotation2160996/range2160996/1
type: Range
persistent identity: range2160996
display id: range2160996
version: 1
direction: direction/forward
top level: BBa_K525562
start: 62
end: 64
orientation: inline

K123000
type: SequenceAnnotation
persistent identity: annotation2161000
display id: annotation2161000
version: 1
top level: BBa_K525562
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K525562/annotation2161000/range2161000/1

https://synbiohub.org/public/igem/BBa_K525562/annotation2161000/range2161000/1
type: Range
persistent identity: range2161000
display id: range2161000
version: 1
direction: direction/forward
top level: BBa_K525562
start: 818
end: 1128
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation2160998
display id: annotation2160998
version: 1
top level: BBa_K525562
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K525562/annotation2160998/range2160998/1

https://synbiohub.org/public/igem/BBa_K525562/annotation2160998/range2160998/1
type: Range
persistent identity: range2160998
display id: range2160998
version: 1
direction: direction/forward
top level: BBa_K525562
start: 44
end: 55
orientation: inline

AgeI
type: SequenceAnnotation
persistent identity: annotation2160993
display id: annotation2160993
version: 1
top level: BBa_K525562
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K525562/annotation2160993/range2160993/1

https://synbiohub.org/public/igem/BBa_K525562/annotation2160993/range2160993/1
type: Range
persistent identity: range2160993
display id: range2160993
version: 1
direction: direction/forward
top level: BBa_K525562
start: 2402
end: 2407
orientation: inline

BisdB
type: SequenceAnnotation
persistent identity: annotation2161294
display id: annotation2161294
version: 1
top level: BBa_K525562
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K525562/annotation2161294/range2161294/1

https://synbiohub.org/public/igem/BBa_K525562/annotation2161294/range2161294/1
type: Range
persistent identity: range2161294
display id: range2161294
version: 1
direction: direction/forward
top level: BBa_K525562
start: 1135
end: 2410
orientation: inline

K123001
type: SequenceAnnotation
persistent identity: annotation2161001
display id: annotation2161001
version: 1
top level: BBa_K525562
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K525562/annotation2161001/range2161001/1

https://synbiohub.org/public/igem/BBa_K525562/annotation2161001/range2161001/1
type: Range
persistent identity: range2161001
display id: range2161001
version: 1
direction: direction/forward
top level: BBa_K525562
start: 1135
end: 2410
orientation: inline

Reductase
type: SequenceAnnotation
persistent identity: annotation2161295
display id: annotation2161295
version: 1
top level: BBa_K525562
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K525562/annotation2161295/range2161295/1

https://synbiohub.org/public/igem/BBa_K525562/annotation2161295/range2161295/1
type: Range
persistent identity: range2161295
display id: range2161295
version: 1
direction: direction/forward
top level: BBa_K525562
start: 162
end: 811
orientation: inline

TAA
type: SequenceAnnotation
persistent identity: annotation2160994
display id: annotation2160994
version: 1
top level: BBa_K525562
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K525562/annotation2160994/range2160994/1

https://synbiohub.org/public/igem/BBa_K525562/annotation2160994/range2160994/1
type: Range
persistent identity: range2160994
display id: range2160994
version: 1
direction: direction/forward
top level: BBa_K525562
start: 2408
end: 2410
orientation: inline

K525499
type: SequenceAnnotation
persistent identity: annotation2160999
display id: annotation2160999
version: 1
top level: BBa_K525562
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K525562/annotation2160999/range2160999/1

https://synbiohub.org/public/igem/BBa_K525562/annotation2160999/range2160999/1
type: Range
persistent identity: range2160999
display id: range2160999
version: 1
direction: direction/forward
top level: BBa_K525562
start: 62
end: 811
orientation: inline

BisdA
type: SequenceAnnotation
persistent identity: annotation2161293
display id: annotation2161293
version: 1
top level: BBa_K525562
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K525562/annotation2161293/range2161293/1

https://synbiohub.org/public/igem/BBa_K525562/annotation2161293/range2161293/1
type: Range
persistent identity: range2161293
display id: range2161293
version: 1
direction: direction/forward
top level: BBa_K525562
start: 818
end: 1128
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2161296
display id: annotation2161296
version: 1
top level: BBa_K525562
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K525562/annotation2161296/range2161296/1

https://synbiohub.org/public/igem/BBa_K525562/annotation2161296/range2161296/1
type: Range
persistent identity: range2161296
display id: range2161296
version: 1
direction: direction/forward
top level: BBa_K525562
start: 47
end: 50
orientation: inline

promoter
type: SequenceAnnotation
persistent identity: annotation2160997
display id: annotation2160997
version: 1
top level: BBa_K525562
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K525562/annotation2160997/range2160997/1

https://synbiohub.org/public/igem/BBa_K525562/annotation2160997/range2160997/1
type: Range
persistent identity: range2160997
display id: range2160997
version: 1
direction: direction/forward
top level: BBa_K525562
start: 1
end: 35
orientation: inline

BBa_K123009
type: ComponentDefinition
persistent identity: BBa K123009
display id: BBa_K123009
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: lacIq->BisdB
creator: Jason Gardiner
created: 2008-10-28T12:00:00Z
modified: 2015-05-08T01:09:44Z
discontinued: false
dominant: false
experience: experience/None
group u list: _241_
m user id: 0
owner id: 2742
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This Construct will allow for the detection and degredation of BPA in cells already containing the ER (K123003) biobrick. It works in two systems first. Together K123003 and K123009 produce a NOT gate where when Bisphenol A (BPA), Estrogen or any other estrogen mimic is in the system the product of K123003 dimerizes and attaches to the ERE site of K123009. This stops the production of TetR (R0040) by phsically stoping the Polymerase from transcribing it. This allows the pTet(C0040)promoter to turn on the production of RFP (E1010) for detection as well as BisdA(K123000) and BisdB(K123001)which allow for the degredation of BPA.
mutable notes: While BisdA and BisdB have only been shown to degrade BPA we have put in RFP to allow for the detection of other estrogen mimics as well.
mutable provenance: Bisphenolics
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_K123002
component: BBa_R0040
component: BBa_E1010
component: BBa_K123008
sequence annotation: BBa_E1010
sequence annotation: BBa_K123002
sequence annotation: BBa_K123008
sequence annotation: BBa_R0040
sequence: https://synbiohub.org/public/igem/BBa_K123009_sequence/1

BBa_K123002
type: Component
persistent identity: component2267219
display id: component2267219
version: 1
top level: BBa_K123009
access: public
definition: https://synbiohub.org/public/igem/BBa_K123002/1

BBa_R0040
type: Component
persistent identity: component2267220
display id: component2267220
version: 1
top level: BBa_K123009
access: public
definition: https://synbiohub.org/public/igem/BBa_R0040/1

BBa_E1010
type: Component
persistent identity: component2267227
display id: component2267227
version: 1
top level: BBa_K123009
access: public
definition: https://synbiohub.org/public/igem/BBa_E1010/1

BBa_K123008
type: Component
persistent identity: component2267240
display id: component2267240
version: 1
top level: BBa_K123009
access: public
definition: BBa_K123008

BBa_E1010
type: SequenceAnnotation
persistent identity: annotation2267227
display id: annotation2267227
version: 1
top level: BBa_K123009
location: https://synbiohub.org/public/igem/BBa_K123009/annotation2267227/range2267227/1
component: BBa_E1010

https://synbiohub.org/public/igem/BBa_K123009/annotation2267227/range2267227/1
type: Range
persistent identity: range2267227
display id: range2267227
version: 1
direction: direction/reverse
top level: BBa_K123009
start: 811
end: 1516
orientation: inline

BBa_K123002
type: SequenceAnnotation
persistent identity: annotation2267219
display id: annotation2267219
version: 1
top level: BBa_K123009
location: https://synbiohub.org/public/igem/BBa_K123009/annotation2267219/range2267219/1
component: BBa_K123002

https://synbiohub.org/public/igem/BBa_K123009/annotation2267219/range2267219/1
type: Range
persistent identity: range2267219
display id: range2267219
version: 1
direction: direction/reverse
top level: BBa_K123009
start: 1
end: 742
orientation: inline

BBa_K123008
type: SequenceAnnotation
persistent identity: annotation2267240
display id: annotation2267240
version: 1
top level: BBa_K123009
location: https://synbiohub.org/public/igem/BBa_K123009/annotation2267240/range2267240/1
component: BBa_K123008

https://synbiohub.org/public/igem/BBa_K123009/annotation2267240/range2267240/1
type: Range
persistent identity: range2267240
display id: range2267240
version: 1
direction: direction/reverse
top level: BBa_K123009
start: 1525
end: 3266
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2267220
display id: annotation2267220
version: 1
top level: BBa_K123009
location: https://synbiohub.org/public/igem/BBa_K123009/annotation2267220/range2267220/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_K123009/annotation2267220/range2267220/1
type: Range
persistent identity: range2267220
display id: range2267220
version: 1
direction: direction/forward
top level: BBa_K123009
start: 751
end: 804
orientation: inline

BBa_K525560
type: ComponentDefinition
persistent identity: BBa K525560
display id: BBa_K525560
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Fusion protein of NADP+ Oxidoreductase and BisdA and BisdB
creator: Anna Drong
created: 2011-09-10T11:00:00Z
modified: 2015-05-08T01:12:34Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _690_
m user id: 0
owner id: 8543
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Fusion protein of NADP+ Oxidoreduktase and BisdA and BisdB; RFC 25
mutable notes: Assembled
mutable provenance: BBa_K525499 and BBa_K123000 and BBa_K123001
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: Scar
sequence annotation: TAA
sequence annotation: ATG
sequence annotation: K525499
sequence annotation: Freiburg Scar
sequence annotation: BisdB
sequence annotation: AgeI
sequence annotation: K123001
sequence annotation: Reductase
sequence annotation: NgoMIV
sequence annotation: K123000
sequence annotation: BisdA
sequence: https://synbiohub.org/public/igem/BBa_K525560_sequence/1

Scar
type: SequenceAnnotation
persistent identity: annotation2161130
display id: annotation2161130
version: 1
top level: BBa_K525560
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K525560/annotation2161130/range2161130/1

https://synbiohub.org/public/igem/BBa_K525560/annotation2161130/range2161130/1
type: Range
persistent identity: range2161130
display id: range2161130
version: 1
direction: direction/forward
top level: BBa_K525560
start: 751
end: 756
orientation: inline

TAA
type: SequenceAnnotation
persistent identity: annotation2161137
display id: annotation2161137
version: 1
top level: BBa_K525560
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K525560/annotation2161137/range2161137/1

https://synbiohub.org/public/igem/BBa_K525560/annotation2161137/range2161137/1
type: Range
persistent identity: range2161137
display id: range2161137
version: 1
direction: direction/forward
top level: BBa_K525560
start: 2347
end: 2349
orientation: inline

ATG
type: SequenceAnnotation
persistent identity: annotation2161128
display id: annotation2161128
version: 1
top level: BBa_K525560
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K525560/annotation2161128/range2161128/1

https://synbiohub.org/public/igem/BBa_K525560/annotation2161128/range2161128/1
type: Range
persistent identity: range2161128
display id: range2161128
version: 1
direction: direction/forward
top level: BBa_K525560
start: 1
end: 3
orientation: inline

K525499
type: SequenceAnnotation
persistent identity: annotation2161127
display id: annotation2161127
version: 1
top level: BBa_K525560
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K525560/annotation2161127/range2161127/1

https://synbiohub.org/public/igem/BBa_K525560/annotation2161127/range2161127/1
type: Range
persistent identity: range2161127
display id: range2161127
version: 1
direction: direction/forward
top level: BBa_K525560
start: 1
end: 750
orientation: inline

Freiburg Scar
type: SequenceAnnotation
persistent identity: annotation2161133
display id: annotation2161133
version: 1
top level: BBa_K525560
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K525560/annotation2161133/range2161133/1

https://synbiohub.org/public/igem/BBa_K525560/annotation2161133/range2161133/1
type: Range
persistent identity: range2161133
display id: range2161133
version: 1
direction: direction/forward
top level: BBa_K525560
start: 1069
end: 1074
orientation: inline

BisdB
type: SequenceAnnotation
persistent identity: annotation2161134
display id: annotation2161134
version: 1
top level: BBa_K525560
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K525560/annotation2161134/range2161134/1

https://synbiohub.org/public/igem/BBa_K525560/annotation2161134/range2161134/1
type: Range
persistent identity: range2161134
display id: range2161134
version: 1
direction: direction/forward
top level: BBa_K525560
start: 1075
end: 2349
orientation: inline

AgeI
type: SequenceAnnotation
persistent identity: annotation2161136
display id: annotation2161136
version: 1
top level: BBa_K525560
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K525560/annotation2161136/range2161136/1

https://synbiohub.org/public/igem/BBa_K525560/annotation2161136/range2161136/1
type: Range
persistent identity: range2161136
display id: range2161136
version: 1
direction: direction/forward
top level: BBa_K525560
start: 2341
end: 2346
orientation: inline

K123001
type: SequenceAnnotation
persistent identity: annotation2161135
display id: annotation2161135
version: 1
top level: BBa_K525560
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K525560/annotation2161135/range2161135/1

https://synbiohub.org/public/igem/BBa_K525560/annotation2161135/range2161135/1
type: Range
persistent identity: range2161135
display id: range2161135
version: 1
direction: direction/forward
top level: BBa_K525560
start: 1075
end: 2349
orientation: inline

Reductase
type: SequenceAnnotation
persistent identity: annotation2161126
display id: annotation2161126
version: 1
top level: BBa_K525560
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K525560/annotation2161126/range2161126/1

https://synbiohub.org/public/igem/BBa_K525560/annotation2161126/range2161126/1
type: Range
persistent identity: range2161126
display id: range2161126
version: 1
direction: direction/forward
top level: BBa_K525560
start: 1
end: 750
orientation: inline

NgoMIV
type: SequenceAnnotation
persistent identity: annotation2161129
display id: annotation2161129
version: 1
top level: BBa_K525560
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K525560/annotation2161129/range2161129/1

https://synbiohub.org/public/igem/BBa_K525560/annotation2161129/range2161129/1
type: Range
persistent identity: range2161129
display id: range2161129
version: 1
direction: direction/forward
top level: BBa_K525560
start: 4
end: 9
orientation: inline

K123000
type: SequenceAnnotation
persistent identity: annotation2161131
display id: annotation2161131
version: 1
top level: BBa_K525560
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K525560/annotation2161131/range2161131/1

https://synbiohub.org/public/igem/BBa_K525560/annotation2161131/range2161131/1
type: Range
persistent identity: range2161131
display id: range2161131
version: 1
direction: direction/forward
top level: BBa_K525560
start: 757
end: 1068
orientation: inline

BisdA
type: SequenceAnnotation
persistent identity: annotation2161132
display id: annotation2161132
version: 1
top level: BBa_K525560
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K525560/annotation2161132/range2161132/1

https://synbiohub.org/public/igem/BBa_K525560/annotation2161132/range2161132/1
type: Range
persistent identity: range2161132
display id: range2161132
version: 1
direction: direction/forward
top level: BBa_K525560
start: 757
end: 1068
orientation: inline

BBa_K123008
type: ComponentDefinition
persistent identity: BBa K123008
display id: BBa_K123008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: BisdA+BisdB
creator: Jason Gardiner
created: 2008-10-27T12:00:00Z
modified: 2015-05-08T01:09:44Z
discontinued: false
dominant: false
group u list: _241_
m user id: 0
owner id: 2742
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part when used will allow the production of TetR to increase the production of BisdA and BisdB. When produced BisdA and BisdB will cause the degredation of Bisphenol A.
mutable notes: N/A
mutable provenance: Bisphenolics
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_K123000
component: BBa_R0040
component: BBa_K123001
sequence annotation: BBa_R0040
sequence annotation: BBa_K123001
sequence annotation: BBa_K123000
sequence: https://synbiohub.org/public/igem/BBa_K123008_sequence/1

BBa_K123000
type: Component
persistent identity: component1992761
display id: component1992761
version: 1
top level: BBa_K123008
access: public
definition: BisdA

BBa_R0040
type: Component
persistent identity: component1992762
display id: component1992762
version: 1
top level: BBa_K123008
access: public
definition: https://synbiohub.org/public/igem/BBa_R0040/1

BBa_R0040
type: Component
persistent identity: component1992756
display id: component1992756
version: 1
top level: BBa_K123008
access: public
definition: https://synbiohub.org/public/igem/BBa_R0040/1

BBa_K123001
type: Component
persistent identity: component1992767
display id: component1992767
version: 1
top level: BBa_K123008
access: public
definition: BisdB

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1992762
display id: annotation1992762
version: 1
top level: BBa_K123008
location: https://synbiohub.org/public/igem/BBa_K123008/annotation1992762/range1992762/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_K123008/annotation1992762/range1992762/1
type: Range
persistent identity: range1992762
display id: range1992762
version: 1
direction: direction/forward
top level: BBa_K123008
start: 399
end: 452
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1992756
display id: annotation1992756
version: 1
top level: BBa_K123008
location: https://synbiohub.org/public/igem/BBa_K123008/annotation1992756/range1992756/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_K123008/annotation1992756/range1992756/1
type: Range
persistent identity: range1992756
display id: range1992756
version: 1
direction: direction/forward
top level: BBa_K123008
start: 1
end: 54
orientation: inline

BBa_K123001
type: SequenceAnnotation
persistent identity: annotation1992767
display id: annotation1992767
version: 1
top level: BBa_K123008
location: https://synbiohub.org/public/igem/BBa_K123008/annotation1992767/range1992767/1
component: BBa_K123001

https://synbiohub.org/public/igem/BBa_K123008/annotation1992767/range1992767/1
type: Range
persistent identity: range1992767
display id: range1992767
version: 1
direction: direction/reverse
top level: BBa_K123008
start: 459
end: 1742
orientation: inline

BBa_K123000
type: SequenceAnnotation
persistent identity: annotation1992761
display id: annotation1992761
version: 1
top level: BBa_K123008
location: https://synbiohub.org/public/igem/BBa_K123008/annotation1992761/range1992761/1
component: BBa_K123000

https://synbiohub.org/public/igem/BBa_K123008/annotation1992761/range1992761/1
type: Range
persistent identity: range1992761
display id: range1992761
version: 1
direction: direction/reverse
top level: BBa_K123008
start: 61
end: 390
orientation: inline

BBa_K525582
type: ComponentDefinition
persistent identity: BBa K525582
display id: BBa_K525582
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Fusion protein of NADP+ Oxidoreductase and BisdA and BisdB, polycistronic
creator: Anna Drong
created: 2011-10-27T11:00:00Z
modified: 2015-05-08T01:12:35Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _690_
m user id: 0
owner id: 8543
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Fusion protein of NADP+ Oxidoreduktase and BisdA and BisdB, policistronic
mutable notes: assembled
mutable provenance: K525499, K12300, K123001
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: J23110
sequence annotation: Freiburg scar
sequence annotation: RBS
sequence annotation: NgoMIV
sequence annotation: B0034
sequence annotation: Ferredoxin
sequence annotation: Ferredoxin-NADP+ oxidoreductase
sequence annotation: ATG
sequence annotation: TAA
sequence annotation: Cytochrome P450
sequence annotation: AgeI
sequence annotation: promoter
sequence annotation: K525515
sequence annotation: K525499
sequence: https://synbiohub.org/public/igem/BBa_K525582_sequence/1

J23110
type: SequenceAnnotation
persistent identity: annotation2161108
display id: annotation2161108
version: 1
top level: BBa_K525582
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K525582/annotation2161108/range2161108/1

https://synbiohub.org/public/igem/BBa_K525582/annotation2161108/range2161108/1
type: Range
persistent identity: range2161108
display id: range2161108
version: 1
direction: direction/forward
top level: BBa_K525582
start: 1
end: 35
orientation: inline

Freiburg scar
type: SequenceAnnotation
persistent identity: annotation2161124
display id: annotation2161124
version: 1
top level: BBa_K525582
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K525582/annotation2161124/range2161124/1

https://synbiohub.org/public/igem/BBa_K525582/annotation2161124/range2161124/1
type: Range
persistent identity: range2161124
display id: range2161124
version: 1
direction: direction/forward
top level: BBa_K525582
start: 1168
end: 1173
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2161107
display id: annotation2161107
version: 1
top level: BBa_K525582
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K525582/annotation2161107/range2161107/1

https://synbiohub.org/public/igem/BBa_K525582/annotation2161107/range2161107/1
type: Range
persistent identity: range2161107
display id: range2161107
version: 1
direction: direction/forward
top level: BBa_K525582
start: 48
end: 51
orientation: inline

NgoMIV
type: SequenceAnnotation
persistent identity: annotation2161112
display id: annotation2161112
version: 1
top level: BBa_K525582
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K525582/annotation2161112/range2161112/1

https://synbiohub.org/public/igem/BBa_K525582/annotation2161112/range2161112/1
type: Range
persistent identity: range2161112
display id: range2161112
version: 1
direction: direction/forward
top level: BBa_K525582
start: 65
end: 70
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation2161115
display id: annotation2161115
version: 1
top level: BBa_K525582
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K525582/annotation2161115/range2161115/1

https://synbiohub.org/public/igem/BBa_K525582/annotation2161115/range2161115/1
type: Range
persistent identity: range2161115
display id: range2161115
version: 1
direction: direction/forward
top level: BBa_K525582
start: 829
end: 840
orientation: inline

Ferredoxin
type: SequenceAnnotation
persistent identity: annotation2161123
display id: annotation2161123
version: 1
top level: BBa_K525582
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K525582/annotation2161123/range2161123/1

https://synbiohub.org/public/igem/BBa_K525582/annotation2161123/range2161123/1
type: Range
persistent identity: range2161123
display id: range2161123
version: 1
direction: direction/forward
top level: BBa_K525582
start: 847
end: 1167
orientation: inline

NgoMIV
type: SequenceAnnotation
persistent identity: annotation2161119
display id: annotation2161119
version: 1
top level: BBa_K525582
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K525582/annotation2161119/range2161119/1

https://synbiohub.org/public/igem/BBa_K525582/annotation2161119/range2161119/1
type: Range
persistent identity: range2161119
display id: range2161119
version: 1
direction: direction/forward
top level: BBa_K525582
start: 852
end: 857
orientation: inline

Ferredoxin-NADP+ oxidoreductase
type: SequenceAnnotation
persistent identity: annotation2161122
display id: annotation2161122
version: 1
top level: BBa_K525582
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K525582/annotation2161122/range2161122/1

https://synbiohub.org/public/igem/BBa_K525582/annotation2161122/range2161122/1
type: Range
persistent identity: range2161122
display id: range2161122
version: 1
direction: direction/forward
top level: BBa_K525582
start: 62
end: 820
orientation: inline

ATG
type: SequenceAnnotation
persistent identity: annotation2161118
display id: annotation2161118
version: 1
top level: BBa_K525582
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K525582/annotation2161118/range2161118/1

https://synbiohub.org/public/igem/BBa_K525582/annotation2161118/range2161118/1
type: Range
persistent identity: range2161118
display id: range2161118
version: 1
direction: direction/forward
top level: BBa_K525582
start: 847
end: 849
orientation: inline

TAA
type: SequenceAnnotation
persistent identity: annotation2161120
display id: annotation2161120
version: 1
top level: BBa_K525582
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K525582/annotation2161120/range2161120/1

https://synbiohub.org/public/igem/BBa_K525582/annotation2161120/range2161120/1
type: Range
persistent identity: range2161120
display id: range2161120
version: 1
direction: direction/forward
top level: BBa_K525582
start: 2446
end: 2448
orientation: inline

ATG
type: SequenceAnnotation
persistent identity: annotation2161111
display id: annotation2161111
version: 1
top level: BBa_K525582
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K525582/annotation2161111/range2161111/1

https://synbiohub.org/public/igem/BBa_K525582/annotation2161111/range2161111/1
type: Range
persistent identity: range2161111
display id: range2161111
version: 1
direction: direction/forward
top level: BBa_K525582
start: 62
end: 64
orientation: inline

Cytochrome P450
type: SequenceAnnotation
persistent identity: annotation2161140
display id: annotation2161140
version: 1
top level: BBa_K525582
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K525582/annotation2161140/range2161140/1

https://synbiohub.org/public/igem/BBa_K525582/annotation2161140/range2161140/1
type: Range
persistent identity: range2161140
display id: range2161140
version: 1
direction: direction/forward
top level: BBa_K525582
start: 1174
end: 2448
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2161116
display id: annotation2161116
version: 1
top level: BBa_K525582
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K525582/annotation2161116/range2161116/1

https://synbiohub.org/public/igem/BBa_K525582/annotation2161116/range2161116/1
type: Range
persistent identity: range2161116
display id: range2161116
version: 1
direction: direction/forward
top level: BBa_K525582
start: 834
end: 837
orientation: inline

AgeI
type: SequenceAnnotation
persistent identity: annotation2161114
display id: annotation2161114
version: 1
top level: BBa_K525582
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K525582/annotation2161114/range2161114/1

https://synbiohub.org/public/igem/BBa_K525582/annotation2161114/range2161114/1
type: Range
persistent identity: range2161114
display id: range2161114
version: 1
direction: direction/forward
top level: BBa_K525582
start: 812
end: 817
orientation: inline

AgeI
type: SequenceAnnotation
persistent identity: annotation2161121
display id: annotation2161121
version: 1
top level: BBa_K525582
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K525582/annotation2161121/range2161121/1

https://synbiohub.org/public/igem/BBa_K525582/annotation2161121/range2161121/1
type: Range
persistent identity: range2161121
display id: range2161121
version: 1
direction: direction/forward
top level: BBa_K525582
start: 2440
end: 2445
orientation: inline

TAA
type: SequenceAnnotation
persistent identity: annotation2161113
display id: annotation2161113
version: 1
top level: BBa_K525582
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K525582/annotation2161113/range2161113/1

https://synbiohub.org/public/igem/BBa_K525582/annotation2161113/range2161113/1
type: Range
persistent identity: range2161113
display id: range2161113
version: 1
direction: direction/forward
top level: BBa_K525582
start: 818
end: 820
orientation: inline

promoter
type: SequenceAnnotation
persistent identity: annotation2161106
display id: annotation2161106
version: 1
top level: BBa_K525582
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K525582/annotation2161106/range2161106/1

https://synbiohub.org/public/igem/BBa_K525582/annotation2161106/range2161106/1
type: Range
persistent identity: range2161106
display id: range2161106
version: 1
direction: direction/forward
top level: BBa_K525582
start: 1
end: 35
orientation: inline

K525515
type: SequenceAnnotation
persistent identity: annotation2161117
display id: annotation2161117
version: 1
top level: BBa_K525582
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K525582/annotation2161117/range2161117/1

https://synbiohub.org/public/igem/BBa_K525582/annotation2161117/range2161117/1
type: Range
persistent identity: range2161117
display id: range2161117
version: 1
direction: direction/forward
top level: BBa_K525582
start: 847
end: 2448
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation2161109
display id: annotation2161109
version: 1
top level: BBa_K525582
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K525582/annotation2161109/range2161109/1

https://synbiohub.org/public/igem/BBa_K525582/annotation2161109/range2161109/1
type: Range
persistent identity: range2161109
display id: range2161109
version: 1
direction: direction/forward
top level: BBa_K525582
start: 44
end: 55
orientation: inline

K525499
type: SequenceAnnotation
persistent identity: annotation2161110
display id: annotation2161110
version: 1
top level: BBa_K525582
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K525582/annotation2161110/range2161110/1

https://synbiohub.org/public/igem/BBa_K525582/annotation2161110/range2161110/1
type: Range
persistent identity: range2161110
display id: range2161110
version: 1
direction: direction/forward
top level: BBa_K525582
start: 62
end: 820
orientation: inline

BBa_K525512
type: ComponentDefinition
persistent identity: BBa K525512
display id: BBa_K525512
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Polycistronic expression of BisdA and BisdB
creator: Jonas Aretz
created: 2011-09-13T11:00:00Z
modified: 2015-05-08T01:12:34Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _690_
m user id: 0
owner id: 6670
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Polycistronic expression of BisdA and BisdB
mutable notes: Polycistronic expression of BisdA and BisdB
mutable provenance: Assembly
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K123000
component: BBa_K123001
component: BBa_B0034
component: BBa_J23110
sequence annotation: BBa_B0034
sequence annotation: BBa_J23110
sequence annotation: BBa_K123001
sequence annotation: BBa_K123000
sequence: https://synbiohub.org/public/igem/BBa_K525512_sequence/1

BBa_K123000
type: Component
persistent identity: component2128165
display id: component2128165
version: 1
top level: BBa_K525512
access: public
definition: BisdA

BBa_K123001
type: Component
persistent identity: component2128168
display id: component2128168
version: 1
top level: BBa_K525512
access: public
definition: BisdB

BBa_B0034
type: Component
persistent identity: component2128164
display id: component2128164
version: 1
top level: BBa_K525512
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0034
type: Component
persistent identity: component2128167
display id: component2128167
version: 1
top level: BBa_K525512
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_J23110
type: Component
persistent identity: component2128162
display id: component2128162
version: 1
top level: BBa_K525512
access: public
definition: https://synbiohub.org/public/igem/BBa_J23110/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2128167
display id: annotation2128167
version: 1
top level: BBa_K525512
location: https://synbiohub.org/public/igem/BBa_K525512/annotation2128167/range2128167/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K525512/annotation2128167/range2128167/1
type: Range
persistent identity: range2128167
display id: range2128167
version: 1
direction: direction/reverse
top level: BBa_K525512
start: 400
end: 411
orientation: inline

BBa_J23110
type: SequenceAnnotation
persistent identity: annotation2128162
display id: annotation2128162
version: 1
top level: BBa_K525512
location: https://synbiohub.org/public/igem/BBa_K525512/annotation2128162/range2128162/1
component: BBa_J23110

https://synbiohub.org/public/igem/BBa_K525512/annotation2128162/range2128162/1
type: Range
persistent identity: range2128162
display id: range2128162
version: 1
direction: direction/reverse
top level: BBa_K525512
start: 1
end: 35
orientation: inline

BBa_K123001
type: SequenceAnnotation
persistent identity: annotation2128168
display id: annotation2128168
version: 1
top level: BBa_K525512
location: https://synbiohub.org/public/igem/BBa_K525512/annotation2128168/range2128168/1
component: BBa_K123001

https://synbiohub.org/public/igem/BBa_K525512/annotation2128168/range2128168/1
type: Range
persistent identity: range2128168
display id: range2128168
version: 1
direction: direction/reverse
top level: BBa_K525512
start: 418
end: 1701
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2128164
display id: annotation2128164
version: 1
top level: BBa_K525512
location: https://synbiohub.org/public/igem/BBa_K525512/annotation2128164/range2128164/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K525512/annotation2128164/range2128164/1
type: Range
persistent identity: range2128164
display id: range2128164
version: 1
direction: direction/reverse
top level: BBa_K525512
start: 44
end: 55
orientation: inline

BBa_K123000
type: SequenceAnnotation
persistent identity: annotation2128165
display id: annotation2128165
version: 1
top level: BBa_K525512
location: https://synbiohub.org/public/igem/BBa_K525512/annotation2128165/range2128165/1
component: BBa_K123000

https://synbiohub.org/public/igem/BBa_K525512/annotation2128165/range2128165/1
type: Range
persistent identity: range2128165
display id: range2128165
version: 1
direction: direction/reverse
top level: BBa_K525512
start: 62
end: 391
orientation: inline

BBa_K525001
type: ComponentDefinition
persistent identity: BBa K525001
display id: BBa_K525001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Fusion protein of SgsE, NADP+ Oxidoreductase, BisdA and BisdB with PT7/lac
creator: Anna Drong
created: 2011-09-10T11:00:00Z
modified: 2015-05-08T01:12:34Z
discontinued: false
dominant: false
experience: experience/None
group u list: _690_
m user id: 0
owner id: 8543
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: PT7 + RBS + SgsE || Red || BisdA || BisdB ; RCU 25
mutable notes: Assembled RCU 25
mutable provenance: BBa_K525303, BBa_K525499, BBa_K123000, BBa_K123001
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: RBS
sequence annotation: Freiburg Scar
sequence annotation: Reductase
sequence annotation: lac operator
sequence annotation: ATG
sequence annotation: K525560
sequence annotation: BisdA
sequence annotation: T7 promoter
sequence annotation: SgsE domain
sequence annotation: AgeI
sequence annotation: K525301
sequence annotation: BisdB
sequence annotation: B0034
sequence annotation: TAA
sequence: https://synbiohub.org/public/igem/BBa_K525001_sequence/1

RBS
type: SequenceAnnotation
persistent identity: annotation2160960
display id: annotation2160960
version: 1
top level: BBa_K525001
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K525001/annotation2160960/range2160960/1

https://synbiohub.org/public/igem/BBa_K525001/annotation2160960/range2160960/1
type: Range
persistent identity: range2160960
display id: range2160960
version: 1
direction: direction/forward
top level: BBa_K525001
start: 56
end: 59
orientation: inline

Freiburg Scar
type: SequenceAnnotation
persistent identity: annotation2160989
display id: annotation2160989
version: 1
top level: BBa_K525001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K525001/annotation2160989/range2160989/1

https://synbiohub.org/public/igem/BBa_K525001/annotation2160989/range2160989/1
type: Range
persistent identity: range2160989
display id: range2160989
version: 1
direction: direction/forward
top level: BBa_K525001
start: 3466
end: 3471
orientation: inline

Reductase
type: SequenceAnnotation
persistent identity: annotation2161003
display id: annotation2161003
version: 1
top level: BBa_K525001
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K525001/annotation2161003/range2161003/1

https://synbiohub.org/public/igem/BBa_K525001/annotation2161003/range2161003/1
type: Range
persistent identity: range2161003
display id: range2161003
version: 1
direction: direction/forward
top level: BBa_K525001
start: 2407
end: 3147
orientation: inline

lac operator
type: SequenceAnnotation
persistent identity: annotation2160958
display id: annotation2160958
version: 1
top level: BBa_K525001
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K525001/annotation2160958/range2160958/1

https://synbiohub.org/public/igem/BBa_K525001/annotation2160958/range2160958/1
type: Range
persistent identity: range2160958
display id: range2160958
version: 1
direction: direction/forward
top level: BBa_K525001
start: 20
end: 44
orientation: inline

ATG
type: SequenceAnnotation
persistent identity: annotation2160961
display id: annotation2160961
version: 1
top level: BBa_K525001
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K525001/annotation2160961/range2160961/1

https://synbiohub.org/public/igem/BBa_K525001/annotation2160961/range2160961/1
type: Range
persistent identity: range2160961
display id: range2160961
version: 1
direction: direction/forward
top level: BBa_K525001
start: 73
end: 75
orientation: inline

Freiburg Scar
type: SequenceAnnotation
persistent identity: annotation2160965
display id: annotation2160965
version: 1
top level: BBa_K525001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K525001/annotation2160965/range2160965/1

https://synbiohub.org/public/igem/BBa_K525001/annotation2160965/range2160965/1
type: Range
persistent identity: range2160965
display id: range2160965
version: 1
direction: direction/forward
top level: BBa_K525001
start: 3148
end: 3153
orientation: inline

K525560
type: SequenceAnnotation
persistent identity: annotation2160964
display id: annotation2160964
version: 1
top level: BBa_K525001
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K525001/annotation2160964/range2160964/1

https://synbiohub.org/public/igem/BBa_K525001/annotation2160964/range2160964/1
type: Range
persistent identity: range2160964
display id: range2160964
version: 1
direction: direction/forward
top level: BBa_K525001
start: 2407
end: 4746
orientation: inline

BisdA
type: SequenceAnnotation
persistent identity: annotation2161002
display id: annotation2161002
version: 1
top level: BBa_K525001
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K525001/annotation2161002/range2161002/1

https://synbiohub.org/public/igem/BBa_K525001/annotation2161002/range2161002/1
type: Range
persistent identity: range2161002
display id: range2161002
version: 1
direction: direction/forward
top level: BBa_K525001
start: 3154
end: 3465
orientation: inline

T7 promoter
type: SequenceAnnotation
persistent identity: annotation2160957
display id: annotation2160957
version: 1
top level: BBa_K525001
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K525001/annotation2160957/range2160957/1

https://synbiohub.org/public/igem/BBa_K525001/annotation2160957/range2160957/1
type: Range
persistent identity: range2160957
display id: range2160957
version: 1
direction: direction/forward
top level: BBa_K525001
start: 1
end: 20
orientation: inline

Freiburg Scar
type: SequenceAnnotation
persistent identity: annotation2160963
display id: annotation2160963
version: 1
top level: BBa_K525001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K525001/annotation2160963/range2160963/1

https://synbiohub.org/public/igem/BBa_K525001/annotation2160963/range2160963/1
type: Range
persistent identity: range2160963
display id: range2160963
version: 1
direction: direction/forward
top level: BBa_K525001
start: 2401
end: 2406
orientation: inline

SgsE domain
type: SequenceAnnotation
persistent identity: annotation2161004
display id: annotation2161004
version: 1
top level: BBa_K525001
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K525001/annotation2161004/range2161004/1

https://synbiohub.org/public/igem/BBa_K525001/annotation2161004/range2161004/1
type: Range
persistent identity: range2161004
display id: range2161004
version: 1
direction: direction/forward
top level: BBa_K525001
start: 76
end: 2400
orientation: inline

AgeI
type: SequenceAnnotation
persistent identity: annotation2160991
display id: annotation2160991
version: 1
top level: BBa_K525001
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K525001/annotation2160991/range2160991/1

https://synbiohub.org/public/igem/BBa_K525001/annotation2160991/range2160991/1
type: Range
persistent identity: range2160991
display id: range2160991
version: 1
direction: direction/forward
top level: BBa_K525001
start: 4738
end: 4743
orientation: inline

K525301
type: SequenceAnnotation
persistent identity: annotation2160962
display id: annotation2160962
version: 1
top level: BBa_K525001
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K525001/annotation2160962/range2160962/1

https://synbiohub.org/public/igem/BBa_K525001/annotation2160962/range2160962/1
type: Range
persistent identity: range2160962
display id: range2160962
version: 1
direction: direction/forward
top level: BBa_K525001
start: 73
end: 2400
orientation: inline

BisdB
type: SequenceAnnotation
persistent identity: annotation2160992
display id: annotation2160992
version: 1
top level: BBa_K525001
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K525001/annotation2160992/range2160992/1

https://synbiohub.org/public/igem/BBa_K525001/annotation2160992/range2160992/1
type: Range
persistent identity: range2160992
display id: range2160992
version: 1
direction: direction/forward
top level: BBa_K525001
start: 3472
end: 4746
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation2160959
display id: annotation2160959
version: 1
top level: BBa_K525001
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K525001/annotation2160959/range2160959/1

https://synbiohub.org/public/igem/BBa_K525001/annotation2160959/range2160959/1
type: Range
persistent identity: range2160959
display id: range2160959
version: 1
direction: direction/forward
top level: BBa_K525001
start: 53
end: 64
orientation: inline

TAA
type: SequenceAnnotation
persistent identity: annotation2160990
display id: annotation2160990
version: 1
top level: BBa_K525001
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K525001/annotation2160990/range2160990/1

https://synbiohub.org/public/igem/BBa_K525001/annotation2160990/range2160990/1
type: Range
persistent identity: range2160990
display id: range2160990
version: 1
direction: direction/forward
top level: BBa_K525001
start: 4744
end: 4746
orientation: inline

BisdB
type: ComponentDefinition
persistent identity: BBa K123001
display id: BBa_K123001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: BisdB
creator: Jason Gardiner
created: 2008-10-27T12:00:00Z
modified: 2015-05-08T01:09:44Z
discontinued: false
dominant: false
experience: experience/None
group u list: _241_
m user id: 0
owner id: 2742
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: When used in conjunction with BisdA this enzyme can be used to degrade Bisphenol A. Activity has previously been recorded in E.coli by Miho Sasaki1 et al. 2005. Miho Sasaki1, Jun-ichi Maki, Ko-ichi Oshiman, Yoshinobu Matsumura1, and Tetsuaki Tsuchido.2005, Biodegradation of bisphenol A by cells and cell lysate from Sphingomonas sp. strain AO1. Biodegredation 16: 449-459
mutable notes: When used with BisdA the degredation of Bisphenol A sharply increases
mutable provenance: Sphingomonas sp. strain AO1
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K123001_sequence/1

BBa_K123005
type: ComponentDefinition
persistent identity: BBa K123005
display id: BBa_K123005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pTetBisdA
creator: Jason Gardiner
created: 2008-10-27T12:00:00Z
modified: 2015-05-08T01:09:44Z
discontinued: false
dominant: false
group u list: _241_
m user id: 0
owner id: 2742
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part has been designed to allow the presence/absence of TetR to control the production of BisdA
mutable notes: N/A
mutable provenance: Bisphenolics
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_R0040
component: BBa_K123000
sequence annotation: BBa_K123000
sequence annotation: BBa_R0040
sequence: https://synbiohub.org/public/igem/BBa_K123005_sequence/1

BBa_R0040
type: Component
persistent identity: component1992744
display id: component1992744
version: 1
top level: BBa_K123005
access: public
definition: https://synbiohub.org/public/igem/BBa_R0040/1

BBa_K123000
type: Component
persistent identity: component1992749
display id: component1992749
version: 1
top level: BBa_K123005
access: public
definition: BisdA

BBa_K123000
type: SequenceAnnotation
persistent identity: annotation1992749
display id: annotation1992749
version: 1
top level: BBa_K123005
location: https://synbiohub.org/public/igem/BBa_K123005/annotation1992749/range1992749/1
component: BBa_K123000

https://synbiohub.org/public/igem/BBa_K123005/annotation1992749/range1992749/1
type: Range
persistent identity: range1992749
display id: range1992749
version: 1
direction: direction/reverse
top level: BBa_K123005
start: 61
end: 390
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1992744
display id: annotation1992744
version: 1
top level: BBa_K123005
location: https://synbiohub.org/public/igem/BBa_K123005/annotation1992744/range1992744/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_K123005/annotation1992744/range1992744/1
type: Range
persistent identity: range1992744
display id: range1992744
version: 1
direction: direction/forward
top level: BBa_K123005
start: 1
end: 54
orientation: inline

BisdA
type: ComponentDefinition
persistent identity: BBa K123000
display id: BBa_K123000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: BisdA
creator: Jason Gardiner
created: 2008-10-27T12:00:00Z
modified: 2015-05-08T01:09:44Z
discontinued: false
dominant: false
experience: experience/None
group u list: _241_
m user id: 0
owner id: 2742
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: When used in conjunction with BisdB this enzyme can be used to degrade Bisphenol A. Activity has previously been recorded in E.coli by Miho Sasaki1 et al. 2005. Miho Sasaki1, Jun-ichi Maki, Ko-ichi Oshiman, Yoshinobu Matsumura1, and Tetsuaki Tsuchido.2005, Biodegradation of bisphenol A by cells and cell lysate from Sphingomonas sp. strain AO1. Biodegredation 16: 449-459
mutable notes: These parts where designed to include a his tag to make it easier to view enzyme presence
mutable provenance: Sphingomonas sp. strain AO1
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K123000_sequence/1

BBa_K525551
type: ComponentDefinition
persistent identity: BBa K525551
display id: BBa_K525551
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: NADP+ Oxidoreductase and polycistronic BisdA and BisdB
creator: Anna Drong
created: 2011-09-10T11:00:00Z
modified: 2015-05-08T01:12:34Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _690_
m user id: 0
owner id: 8543
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: NADP+ Oxidoreduktase and polycistronic BisdA and BisdB
mutable notes: Assembled
mutable provenance: BBa_K52499, BBa_K123000, BBa_123001, B0034
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: TAA
sequence annotation: BisdB
sequence annotation: ATG
sequence annotation: RBS
sequence annotation: NgoMIV
sequence annotation: AgeI
sequence annotation: K123000
sequence annotation: K123001
sequence annotation: B0034
sequence annotation: BisdA
sequence annotation: K525499
sequence annotation: Reductase
sequence: https://synbiohub.org/public/igem/BBa_K525551_sequence/1

TAA
type: SequenceAnnotation
persistent identity: annotation2129887
display id: annotation2129887
version: 1
top level: BBa_K525551
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K525551/annotation2129887/range2129887/1

https://synbiohub.org/public/igem/BBa_K525551/annotation2129887/range2129887/1
type: Range
persistent identity: range2129887
display id: range2129887
version: 1
direction: direction/forward
top level: BBa_K525551
start: 1113
end: 1115
orientation: inline

BisdB
type: SequenceAnnotation
persistent identity: annotation2129892
display id: annotation2129892
version: 1
top level: BBa_K525551
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K525551/annotation2129892/range2129892/1

https://synbiohub.org/public/igem/BBa_K525551/annotation2129892/range2129892/1
type: Range
persistent identity: range2129892
display id: range2129892
version: 1
direction: direction/forward
top level: BBa_K525551
start: 1142
end: 2425
orientation: inline

ATG
type: SequenceAnnotation
persistent identity: annotation2129890
display id: annotation2129890
version: 1
top level: BBa_K525551
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K525551/annotation2129890/range2129890/1

https://synbiohub.org/public/igem/BBa_K525551/annotation2129890/range2129890/1
type: Range
persistent identity: range2129890
display id: range2129890
version: 1
direction: direction/forward
top level: BBa_K525551
start: 1142
end: 1144
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2129881
display id: annotation2129881
version: 1
top level: BBa_K525551
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K525551/annotation2129881/range2129881/1

https://synbiohub.org/public/igem/BBa_K525551/annotation2129881/range2129881/1
type: Range
persistent identity: range2129881
display id: range2129881
version: 1
direction: direction/forward
top level: BBa_K525551
start: 771
end: 774
orientation: inline

NgoMIV
type: SequenceAnnotation
persistent identity: annotation2129877
display id: annotation2129877
version: 1
top level: BBa_K525551
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K525551/annotation2129877/range2129877/1

https://synbiohub.org/public/igem/BBa_K525551/annotation2129877/range2129877/1
type: Range
persistent identity: range2129877
display id: range2129877
version: 1
direction: direction/forward
top level: BBa_K525551
start: 4
end: 9
orientation: inline

ATG
type: SequenceAnnotation
persistent identity: annotation2129875
display id: annotation2129875
version: 1
top level: BBa_K525551
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K525551/annotation2129875/range2129875/1

https://synbiohub.org/public/igem/BBa_K525551/annotation2129875/range2129875/1
type: Range
persistent identity: range2129875
display id: range2129875
version: 1
direction: direction/forward
top level: BBa_K525551
start: 1
end: 3
orientation: inline

AgeI
type: SequenceAnnotation
persistent identity: annotation2129878
display id: annotation2129878
version: 1
top level: BBa_K525551
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K525551/annotation2129878/range2129878/1

https://synbiohub.org/public/igem/BBa_K525551/annotation2129878/range2129878/1
type: Range
persistent identity: range2129878
display id: range2129878
version: 1
direction: direction/forward
top level: BBa_K525551
start: 751
end: 756
orientation: inline

TAA
type: SequenceAnnotation
persistent identity: annotation2129895
display id: annotation2129895
version: 1
top level: BBa_K525551
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K525551/annotation2129895/range2129895/1

https://synbiohub.org/public/igem/BBa_K525551/annotation2129895/range2129895/1
type: Range
persistent identity: range2129895
display id: range2129895
version: 1
direction: direction/forward
top level: BBa_K525551
start: 2423
end: 2425
orientation: inline

TAA
type: SequenceAnnotation
persistent identity: annotation2129879
display id: annotation2129879
version: 1
top level: BBa_K525551
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K525551/annotation2129879/range2129879/1

https://synbiohub.org/public/igem/BBa_K525551/annotation2129879/range2129879/1
type: Range
persistent identity: range2129879
display id: range2129879
version: 1
direction: direction/forward
top level: BBa_K525551
start: 757
end: 759
orientation: inline

K123000
type: SequenceAnnotation
persistent identity: annotation2129882
display id: annotation2129882
version: 1
top level: BBa_K525551
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K525551/annotation2129882/range2129882/1

https://synbiohub.org/public/igem/BBa_K525551/annotation2129882/range2129882/1
type: Range
persistent identity: range2129882
display id: range2129882
version: 1
direction: direction/forward
top level: BBa_K525551
start: 786
end: 1115
orientation: inline

K123001
type: SequenceAnnotation
persistent identity: annotation2129891
display id: annotation2129891
version: 1
top level: BBa_K525551
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K525551/annotation2129891/range2129891/1

https://synbiohub.org/public/igem/BBa_K525551/annotation2129891/range2129891/1
type: Range
persistent identity: range2129891
display id: range2129891
version: 1
direction: direction/forward
top level: BBa_K525551
start: 1142
end: 2425
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2129889
display id: annotation2129889
version: 1
top level: BBa_K525551
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K525551/annotation2129889/range2129889/1

https://synbiohub.org/public/igem/BBa_K525551/annotation2129889/range2129889/1
type: Range
persistent identity: range2129889
display id: range2129889
version: 1
direction: direction/forward
top level: BBa_K525551
start: 1127
end: 1130
orientation: inline

NgoMIV
type: SequenceAnnotation
persistent identity: annotation2129885
display id: annotation2129885
version: 1
top level: BBa_K525551
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K525551/annotation2129885/range2129885/1

https://synbiohub.org/public/igem/BBa_K525551/annotation2129885/range2129885/1
type: Range
persistent identity: range2129885
display id: range2129885
version: 1
direction: direction/forward
top level: BBa_K525551
start: 789
end: 794
orientation: inline

AgeI
type: SequenceAnnotation
persistent identity: annotation2129894
display id: annotation2129894
version: 1
top level: BBa_K525551
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K525551/annotation2129894/range2129894/1

https://synbiohub.org/public/igem/BBa_K525551/annotation2129894/range2129894/1
type: Range
persistent identity: range2129894
display id: range2129894
version: 1
direction: direction/forward
top level: BBa_K525551
start: 2417
end: 2422
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation2129888
display id: annotation2129888
version: 1
top level: BBa_K525551
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K525551/annotation2129888/range2129888/1

https://synbiohub.org/public/igem/BBa_K525551/annotation2129888/range2129888/1
type: Range
persistent identity: range2129888
display id: range2129888
version: 1
direction: direction/forward
top level: BBa_K525551
start: 1124
end: 1135
orientation: inline

AgeI
type: SequenceAnnotation
persistent identity: annotation2129886
display id: annotation2129886
version: 1
top level: BBa_K525551
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K525551/annotation2129886/range2129886/1

https://synbiohub.org/public/igem/BBa_K525551/annotation2129886/range2129886/1
type: Range
persistent identity: range2129886
display id: range2129886
version: 1
direction: direction/forward
top level: BBa_K525551
start: 1107
end: 1112
orientation: inline

BisdA
type: SequenceAnnotation
persistent identity: annotation2129884
display id: annotation2129884
version: 1
top level: BBa_K525551
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K525551/annotation2129884/range2129884/1

https://synbiohub.org/public/igem/BBa_K525551/annotation2129884/range2129884/1
type: Range
persistent identity: range2129884
display id: range2129884
version: 1
direction: direction/forward
top level: BBa_K525551
start: 786
end: 1115
orientation: inline

ATG
type: SequenceAnnotation
persistent identity: annotation2129883
display id: annotation2129883
version: 1
top level: BBa_K525551
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K525551/annotation2129883/range2129883/1

https://synbiohub.org/public/igem/BBa_K525551/annotation2129883/range2129883/1
type: Range
persistent identity: range2129883
display id: range2129883
version: 1
direction: direction/forward
top level: BBa_K525551
start: 786
end: 788
orientation: inline

K525499
type: SequenceAnnotation
persistent identity: annotation2129874
display id: annotation2129874
version: 1
top level: BBa_K525551
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K525551/annotation2129874/range2129874/1

https://synbiohub.org/public/igem/BBa_K525551/annotation2129874/range2129874/1
type: Range
persistent identity: range2129874
display id: range2129874
version: 1
direction: direction/forward
top level: BBa_K525551
start: 1
end: 759
orientation: inline

NgoMIV
type: SequenceAnnotation
persistent identity: annotation2129893
display id: annotation2129893
version: 1
top level: BBa_K525551
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K525551/annotation2129893/range2129893/1

https://synbiohub.org/public/igem/BBa_K525551/annotation2129893/range2129893/1
type: Range
persistent identity: range2129893
display id: range2129893
version: 1
direction: direction/forward
top level: BBa_K525551
start: 1145
end: 1150
orientation: inline

Reductase
type: SequenceAnnotation
persistent identity: annotation2129876
display id: annotation2129876
version: 1
top level: BBa_K525551
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K525551/annotation2129876/range2129876/1

https://synbiohub.org/public/igem/BBa_K525551/annotation2129876/range2129876/1
type: Range
persistent identity: range2129876
display id: range2129876
version: 1
direction: direction/forward
top level: BBa_K525551
start: 1
end: 759
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation2129880
display id: annotation2129880
version: 1
top level: BBa_K525551
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K525551/annotation2129880/range2129880/1

https://synbiohub.org/public/igem/BBa_K525551/annotation2129880/range2129880/1
type: Range
persistent identity: range2129880
display id: range2129880
version: 1
direction: direction/forward
top level: BBa_K525551
start: 768
end: 779
orientation: inline

BisdAB
type: ComponentDefinition
persistent identity: BBa K525515
display id: BBa_K525515
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Fusion protein of BisdA and BisdB
creator: Anna Drong
created: 2011-09-09T11:00:00Z
modified: 2015-05-08T01:12:34Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _690_
m user id: 0
owner id: 8543
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: Fusion protein of BisdA and BisdB
mutable notes: Assembled
mutable provenance: BBa_K123000 and BBa_K123001
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Freiburg scar
sequence annotation: NgoMIV
sequence annotation: ATG
sequence annotation: K123001
sequence annotation: TAA
sequence annotation: AgeI
sequence annotation: K123000
sequence: https://synbiohub.org/public/igem/BBa_K525515_sequence/1

Freiburg scar
type: SequenceAnnotation
persistent identity: annotation2128161
display id: annotation2128161
version: 1
top level: BisdAB
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K525515/annotation2128161/range2128161/1

https://synbiohub.org/public/igem/BBa_K525515/annotation2128161/range2128161/1
type: Range
persistent identity: range2128161
display id: range2128161
version: 1
direction: direction/forward
top level: BisdAB
start: 322
end: 327
orientation: inline

NgoMIV
type: SequenceAnnotation
persistent identity: annotation2128156
display id: annotation2128156
version: 1
top level: BisdAB
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K525515/annotation2128156/range2128156/1

https://synbiohub.org/public/igem/BBa_K525515/annotation2128156/range2128156/1
type: Range
persistent identity: range2128156
display id: range2128156
version: 1
direction: direction/forward
top level: BisdAB
start: 4
end: 9
orientation: inline

ATG
type: SequenceAnnotation
persistent identity: annotation2128158
display id: annotation2128158
version: 1
top level: BisdAB
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K525515/annotation2128158/range2128158/1

https://synbiohub.org/public/igem/BBa_K525515/annotation2128158/range2128158/1
type: Range
persistent identity: range2128158
display id: range2128158
version: 1
direction: direction/forward
top level: BisdAB
start: 1
end: 3
orientation: inline

K123001
type: SequenceAnnotation
persistent identity: annotation2126910
display id: annotation2126910
version: 1
top level: BisdAB
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K525515/annotation2126910/range2126910/1

https://synbiohub.org/public/igem/BBa_K525515/annotation2126910/range2126910/1
type: Range
persistent identity: range2126910
display id: range2126910
version: 1
direction: direction/forward
top level: BisdAB
start: 328
end: 1602
orientation: inline

TAA
type: SequenceAnnotation
persistent identity: annotation2128159
display id: annotation2128159
version: 1
top level: BisdAB
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K525515/annotation2128159/range2128159/1

https://synbiohub.org/public/igem/BBa_K525515/annotation2128159/range2128159/1
type: Range
persistent identity: range2128159
display id: range2128159
version: 1
direction: direction/forward
top level: BisdAB
start: 1599
end: 1602
orientation: inline

AgeI
type: SequenceAnnotation
persistent identity: annotation2128157
display id: annotation2128157
version: 1
top level: BisdAB
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K525515/annotation2128157/range2128157/1

https://synbiohub.org/public/igem/BBa_K525515/annotation2128157/range2128157/1
type: Range
persistent identity: range2128157
display id: range2128157
version: 1
direction: direction/forward
top level: BisdAB
start: 1594
end: 1599
orientation: inline

K123000
type: SequenceAnnotation
persistent identity: annotation2126909
display id: annotation2126909
version: 1
top level: BisdAB
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K525515/annotation2126909/range2126909/1

https://synbiohub.org/public/igem/BBa_K525515/annotation2126909/range2126909/1
type: Range
persistent identity: range2126909
display id: range2126909
version: 1
direction: direction/forward
top level: BisdAB
start: 1
end: 321
orientation: inline