Fusion protein of S-Layer SgsE with T7/lac and BisdA and BisdB
Fusion protein of S-Layer SbpA with T7/lac and BisdA and BisdB
Fusion protein of BisdA and BisdB behind constitutive promoter
Fusion protein of SbpA, NADP+ Oxidoreductase, BisdA and BisdB with PT7/lac
PT7 + RBS + SbpA || Red || BisdA || BisdB
This part has been designed to allow the presence/absence of TetR to control the production of BisdB
Fusion protein of NADP+ Oxidoreductase and BisdA and BisdB with middle strong promoter,RBS
Fusion protein of ferredoxin-NADP+ oxidoreductase, BisdA and BisdB; RFC 25 (Freiburg BioBrick assembly standard) for characterization of intra- and extracellular BPA degradation.
This Construct will allow for the detection and degredation of BPA in cells already containing the ER (K123003) biobrick. It works in two systems first. Together K123003 and K123009 produce a NOT gate where when Bisphenol A (BPA), Estrogen or any other estrogen mimic is in the system the product of K123003 dimerizes and attaches to the ERE site of K123009. This stops the production of TetR (R0040) by phsically stoping the Polymerase from transcribing it. This allows the pTet(C0040)promoter to turn on the production of RFP (E1010) for detection as well as BisdA(K123000) and BisdB(K123001)which allow for the degredation of BPA.
While BisdA and BisdB have only been shown to degrade BPA we have put in RFP to allow for the detection of other estrogen mimics as well.
Fusion protein of NADP+ Oxidoreductase and BisdA and BisdB
Fusion protein of NADP+ Oxidoreduktase and BisdA and BisdB; RFC 25
This part when used will allow the production of TetR to increase the production of BisdA and BisdB. When produced BisdA and BisdB will cause the degredation of Bisphenol A.
Fusion protein of NADP+ Oxidoreductase and BisdA and BisdB, polycistronic
Fusion protein of NADP+ Oxidoreduktase and BisdA and BisdB, policistronic
Polycistronic expression of BisdA and BisdB
Fusion protein of SgsE, NADP+ Oxidoreductase, BisdA and BisdB with PT7/lac
PT7 + RBS + SgsE || Red || BisdA || BisdB ; RCU 25
When used in conjunction with BisdA this enzyme can be used to degrade Bisphenol A. Activity has previously been recorded in E.coli by Miho Sasaki1 et al. 2005. Miho Sasaki1, Jun-ichi Maki, Ko-ichi Oshiman, Yoshinobu Matsumura1, and Tetsuaki Tsuchido.2005, Biodegradation of bisphenol A by cells and cell lysate from Sphingomonas sp. strain AO1. Biodegredation 16: 449-459
When used with BisdA the degredation of Bisphenol A sharply increases
This part has been designed to allow the presence/absence of TetR to control the production of BisdA
When used in conjunction with BisdB this enzyme can be used to degrade Bisphenol A. Activity has previously been recorded in E.coli by Miho Sasaki1 et al. 2005. Miho Sasaki1, Jun-ichi Maki, Ko-ichi Oshiman, Yoshinobu Matsumura1, and Tetsuaki Tsuchido.2005, Biodegradation of bisphenol A by cells and cell lysate from Sphingomonas sp. strain AO1. Biodegredation 16: 449-459
These parts where designed to include a his tag to make it easier to view enzyme presence
NADP+ Oxidoreductase and polycistronic BisdA and BisdB
NADP+ Oxidoreduktase and polycistronic BisdA and BisdB