//function/degradation/ahl
type: Collection
persistent identity: function degradation ahl
display id: function_degradation_ahl
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
owned by: james
owned by: myers
member: https://synbiohub.org/public/igem/BBa_I724000/1
member: https://synbiohub.org/public/igem/BBa_C0060/1
member: https://synbiohub.org/public/igem/BBa_C0160/1
member: https://synbiohub.org/public/igem/BBa_J22071/1
member: https://synbiohub.org/public/igem/BBa_C0260/1

//function/degradation/cellulose
type: Collection
persistent identity: function degradation cellulose
display id: function_degradation_cellulose
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
owned by: james
owned by: myers
member: https://synbiohub.org/public/igem/BBa_K1044000/1
member: https://synbiohub.org/public/igem/BBa_J15507/1
member: https://synbiohub.org/public/igem/BBa_J15508/1
member: https://synbiohub.org/public/igem/BBa_K633002/1
member: https://synbiohub.org/public/igem/BBa_K805011/1
member: https://synbiohub.org/public/igem/BBa_J15510/1
member: https://synbiohub.org/public/igem/BBa_J15509/1
member: https://synbiohub.org/public/igem/BBa_K118028/1
member: https://synbiohub.org/public/igem/BBa_K633000/1
member: https://synbiohub.org/public/igem/BBa_K118022/1
member: https://synbiohub.org/public/igem/BBa_K118023/1
member: https://synbiohub.org/public/igem/BBa_K633014/1

//function/degradation
type: Collection
persistent identity: function degradation
display id: function_degradation
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
owned by: james
owned by: myers
member: BBa_K078003
member: Nuc-T7
member: BBa_K863010
member: BBa_K137014
member: BBa_K1031620
member: tphB
member: BBa_K078008
member: BBa_K2010000
member: BBa_K863015
member: BBa_K863001
member: BBa_K2010005
member: BBa_K137000
member: BBa_K1031625
member: BBa_K1165002
member: //function/degradation/cellulose
member: BBa_K1031622
member: BBa_K1031923
member: BBa_K137067
member: BBa_K863012
member: BBa_K808025
member: Bt-ALDH
member: //function/degradation/ahl
member: Mdaa
member: BBa_K863005
member: BBa_K1031623
member: BBa_K2010007
member: BBa_K174000
member: BBa_K1031624
member: BBa_K2010008
member: BBa_K1398001
member: BBa_K144000
member: BBa_K863011
member: BBa_K863006
member: XenB
member: BBa_K078102
member: BBa_K1355004
member: BBa_K174002
member: BBa_K2088010
member: BBa_K2010004
member: TetX
member: alkJ
member: BBa_K1031931
member: BBa_K1031621
member: ladA
member: Bioplastic
member: BBa_K1031922
member: BBa_K078009
member: BBa_K808030
member: BBa_K2010001
member: Bt-ADH
member: BBa_K808026
member: BBa_K863000
member: BBa_K1149006
member: BBa_K338022
member: BBa_K863021
member: BBa_K1165003
member: BBa_K2010002
member: BBa_K078005
member: BBa_K078006
member: BBa_K863020
member: BBa_K1165004
member: BBa_K2010003
member: BBa_K078007
member: BBa_I716253
member: //function/degradation/bisphenol
member: BBa_K338003

//function/degradation/bisphenol
type: Collection
persistent identity: function degradation bisphenol
display id: function_degradation_bisphenol
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
owned by: james
owned by: myers
member: https://synbiohub.org/public/igem/BBa_K525517/1
member: https://synbiohub.org/public/igem/BBa_K525582/1
member: https://synbiohub.org/public/igem/BBa_K123005/1
member: https://synbiohub.org/public/igem/BBa_K525001/1
member: https://synbiohub.org/public/igem/BBa_K525560/1
member: https://synbiohub.org/public/igem/BBa_K123007/1
member: https://synbiohub.org/public/igem/BBa_K525007/1
member: https://synbiohub.org/public/igem/BBa_K525515/1
member: https://synbiohub.org/public/igem/BBa_K123001/1
member: https://synbiohub.org/public/igem/BBa_K525562/1
member: https://synbiohub.org/public/igem/BBa_K123009/1
member: https://synbiohub.org/public/igem/BBa_K525008/1
member: https://synbiohub.org/public/igem/BBa_K525551/1
member: https://synbiohub.org/public/igem/BBa_K123008/1
member: https://synbiohub.org/public/igem/BBa_K123000/1
member: https://synbiohub.org/public/igem/BBa_K525512/1
member: https://synbiohub.org/public/igem/BBa_K525002/1

BBa_K144000
type: ComponentDefinition
persistent identity: BBa K144000
display id: BBa_K144000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Lignin Peroxidase A
creator: Caleb Dulaney
created: 2008-10-27T12:00:00Z
modified: 2015-05-08T01:10:26Z
discontinued: false
dominant: false
group u list: _207_
m user id: 0
owner id: 2074
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part codes for the enzyme Lignin Peroxidase which initiates the breakdown of lignin, a complex biopolymer in biomass. The part is currently in testing, but should produce the active enzyme. It can be used in bioenergy projects to begin the process of making biofuels. Also, there has been some discussion about the possibility of using peroxidases to break down synthetic polymers.
mutable notes: This part is one of a family of highly homologous genes in P.C. It was nearly impossible to isolate the gene using primers specific for the coding sequence. It was necessary to use a sequential set of PCR primers specific for the upstream and downstream regions and then the specific coding sequence, respectively.
mutable provenance: cDNA of Phanerochaete Chrysosporium grown on Aspen Wood Culture at University of Wisconsin/USDA Forest Products Lab.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K144000_sequence/1

BBa_K1165004
type: ComponentDefinition
persistent identity: BBa K1165004
display id: BBa_K1165004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PenI-cleaving Ribozyme 1 of XOR Gate 2
creator: iGEM Concordia 2013
created: 2013-09-26T11:00:00Z
modified: 2015-05-08T01:09:33Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1477_
m user id: 0
owner id: 18237
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: -
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000834
role: partType/RNA
sequence annotation: TAA Bump
sequence annotation: Target Binding Arms
sequence annotation: Target-Binding Arms
sequence annotation: Synthetic Extended Arms
sequence annotation: Catalytic Core
sequence: https://synbiohub.org/public/igem/BBa_K1165004_sequence/1

TAA Bump
type: SequenceAnnotation
persistent identity: annotation2365273
display id: annotation2365273
version: 1
top level: BBa_K1165004
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1165004/annotation2365273/range2365273/1

https://synbiohub.org/public/igem/BBa_K1165004/annotation2365273/range2365273/1
type: Range
persistent identity: range2365273
display id: range2365273
version: 1
direction: direction/forward
top level: BBa_K1165004
start: 36
end: 38
orientation: inline

Target Binding Arms
type: SequenceAnnotation
persistent identity: annotation2365277
display id: annotation2365277
version: 1
top level: BBa_K1165004
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1165004/annotation2365277/range2365277/1

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type: Range
persistent identity: range2365277
display id: range2365277
version: 1
direction: direction/forward
top level: BBa_K1165004
start: 39
end: 43
orientation: inline

Target-Binding Arms
type: SequenceAnnotation
persistent identity: annotation2365272
display id: annotation2365272
version: 1
top level: BBa_K1165004
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1165004/annotation2365272/range2365272/1

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type: Range
persistent identity: range2365272
display id: range2365272
version: 1
direction: direction/forward
top level: BBa_K1165004
start: 33
end: 35
orientation: inline

Synthetic Extended Arms
type: SequenceAnnotation
persistent identity: annotation2365271
display id: annotation2365271
version: 1
top level: BBa_K1165004
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1165004/annotation2365271/range2365271/1

https://synbiohub.org/public/igem/BBa_K1165004/annotation2365271/range2365271/1
type: Range
persistent identity: range2365271
display id: range2365271
version: 1
direction: direction/forward
top level: BBa_K1165004
start: 1
end: 30
orientation: inline

Synthetic Extended Arms
type: SequenceAnnotation
persistent identity: annotation2365280
display id: annotation2365280
version: 1
top level: BBa_K1165004
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1165004/annotation2365280/range2365280/1

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type: Range
persistent identity: range2365280
display id: range2365280
version: 1
direction: direction/forward
top level: BBa_K1165004
start: 82
end: 111
orientation: inline

Catalytic Core
type: SequenceAnnotation
persistent identity: annotation2365278
display id: annotation2365278
version: 1
top level: BBa_K1165004
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1165004/annotation2365278/range2365278/1

https://synbiohub.org/public/igem/BBa_K1165004/annotation2365278/range2365278/1
type: Range
persistent identity: range2365278
display id: range2365278
version: 1
direction: direction/forward
top level: BBa_K1165004
start: 44
end: 69
orientation: inline

Target-Binding Arms
type: SequenceAnnotation
persistent identity: annotation2365279
display id: annotation2365279
version: 1
top level: BBa_K1165004
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1165004/annotation2365279/range2365279/1

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type: Range
persistent identity: range2365279
display id: range2365279
version: 1
direction: direction/forward
top level: BBa_K1165004
start: 70
end: 81
orientation: inline

BBa_K1031624
type: ComponentDefinition
persistent identity: BBa K1031624
display id: BBa_K1031624
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: J23114-NahF-Terminator
creator: He Shuaixin
created: 2013-09-08T11:00:00Z
modified: 2015-05-08T01:08:47Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1338_
m user id: 0
owner id: 15843
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: 114-NahF-TT
mutable notes: molecular cloning
mutable provenance: synthesis Pseudomonas
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: NahF
sequence annotation: B0015
sequence annotation: J23114
sequence: https://synbiohub.org/public/igem/BBa_K1031624_sequence/1

NahF
type: SequenceAnnotation
persistent identity: annotation2361904
display id: annotation2361904
version: 1
top level: BBa_K1031624
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1031624/annotation2361904/range2361904/1

https://synbiohub.org/public/igem/BBa_K1031624/annotation2361904/range2361904/1
type: Range
persistent identity: range2361904
display id: range2361904
version: 1
direction: direction/forward
top level: BBa_K1031624
start: 62
end: 1513
orientation: inline

B0015
type: SequenceAnnotation
persistent identity: annotation2361905
display id: annotation2361905
version: 1
top level: BBa_K1031624
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1031624/annotation2361905/range2361905/1

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type: Range
persistent identity: range2361905
display id: range2361905
version: 1
direction: direction/forward
top level: BBa_K1031624
start: 1520
end: 1648
orientation: inline

J23114
type: SequenceAnnotation
persistent identity: annotation2361903
display id: annotation2361903
version: 1
top level: BBa_K1031624
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1031624/annotation2361903/range2361903/1

https://synbiohub.org/public/igem/BBa_K1031624/annotation2361903/range2361903/1
type: Range
persistent identity: range2361903
display id: range2361903
version: 1
direction: direction/forward
top level: BBa_K1031624
start: 1
end: 35
orientation: inline

BBa_K338003
type: ComponentDefinition
persistent identity: BBa K338003
display id: BBa_K338003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PHA Synthase Composite, Part 1/2
creator: Caltech iGEM 2010
created: 2010-10-25T11:00:00Z
modified: 2015-05-08T01:12:07Z
discontinued: false
dominant: false
experience: experience/None
group u list: _454_
m user id: 0
owner id: 6013
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This is half of a planned part which would contain all three PHA synthase genes required to produce [http://en.wikipedia.org/wiki/Polyhydroxybutyrate polyhydroxybutyrate] (PHB) in cells: ''phaA'', ''phaB1'', ''phaC1''. This half contains only ''phaA'': <partinfo>BBa_K156012</partinfo>. It was designed to be ligated upstream of part BBa_K338004.
mutable notes: When ligated upstream of BBa_K338004, the completed construct was designed to express all three PHA synthase genes required to make PHB oligomers from soybean oil. The three genes would be transcribed polycistronically on a single mRNA transcript under the IPTG-inducible control of the <partinfo>BBa_K215000</partinfo> promoter. Naturally, each gene is preceded by a standard RBS (<partinfo>B0034</partinfo>) and the transcript finishes with a strong terminator (<partinfo>B0015</partinfo>), for a total size of about 3500bp. Note that these three genes should only cause the production of PHB ''oligomers'' in cells, not hardened plastic. A crosslinking agent is required to link the oligomers and form the final plastic product. Over-expression of the ''phaC1'' gene could cause some crosslinking, but this has not been experimentally verified.
mutable provenance: Registry of Standard Biological Parts
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K156012
component: BBa_B0034
component: BBa_K215000
sequence annotation: BBa_K156012
sequence annotation: BBa_B0034
sequence annotation: BBa_K215000
sequence: https://synbiohub.org/public/igem/BBa_K338003_sequence/1

BBa_K156012
type: Component
persistent identity: component2101446
display id: component2101446
version: 1
top level: BBa_K338003
access: public
definition: Bioplastic

BBa_B0034
type: Component
persistent identity: component2101445
display id: component2101445
version: 1
top level: BBa_K338003
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0034
type: Component
persistent identity: component2101448
display id: component2101448
version: 1
top level: BBa_K338003
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K215000
type: Component
persistent identity: component2101443
display id: component2101443
version: 1
top level: BBa_K338003
access: public
definition: https://synbiohub.org/public/igem/BBa_K215000/1

BBa_K156012
type: SequenceAnnotation
persistent identity: annotation2101446
display id: annotation2101446
version: 1
top level: BBa_K338003
location: https://synbiohub.org/public/igem/BBa_K338003/annotation2101446/range2101446/1
component: BBa_K156012

https://synbiohub.org/public/igem/BBa_K338003/annotation2101446/range2101446/1
type: Range
persistent identity: range2101446
display id: range2101446
version: 1
direction: direction/reverse
top level: BBa_K338003
start: 102
end: 1283
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2101445
display id: annotation2101445
version: 1
top level: BBa_K338003
location: https://synbiohub.org/public/igem/BBa_K338003/annotation2101445/range2101445/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K338003/annotation2101445/range2101445/1
type: Range
persistent identity: range2101445
display id: range2101445
version: 1
direction: direction/reverse
top level: BBa_K338003
start: 84
end: 95
orientation: inline

BBa_K215000
type: SequenceAnnotation
persistent identity: annotation2101443
display id: annotation2101443
version: 1
top level: BBa_K338003
location: https://synbiohub.org/public/igem/BBa_K338003/annotation2101443/range2101443/1
component: BBa_K215000

https://synbiohub.org/public/igem/BBa_K338003/annotation2101443/range2101443/1
type: Range
persistent identity: range2101443
display id: range2101443
version: 1
direction: direction/reverse
top level: BBa_K338003
start: 1
end: 75
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2101448
display id: annotation2101448
version: 1
top level: BBa_K338003
location: https://synbiohub.org/public/igem/BBa_K338003/annotation2101448/range2101448/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K338003/annotation2101448/range2101448/1
type: Range
persistent identity: range2101448
display id: range2101448
version: 1
direction: direction/reverse
top level: BBa_K338003
start: 1292
end: 1303
orientation: inline

BBa_K1165003
type: ComponentDefinition
persistent identity: BBa K1165003
display id: BBa_K1165003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PenI-cleaving Ribozyme 2 of XOR Gate 1
creator: iGEM Concordia 2013
created: 2013-09-26T11:00:00Z
modified: 2015-05-08T01:09:33Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1477_
m user id: 0
owner id: 18237
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: -
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000834
role: partType/RNA
sequence annotation: Catalytic Core
sequence annotation: Target Binding Arms
sequence annotation: Synthetic Extended Arms
sequence annotation: Target-Binding Arms
sequence annotation: TAA Bump
sequence: https://synbiohub.org/public/igem/BBa_K1165003_sequence/1

Catalytic Core
type: SequenceAnnotation
persistent identity: annotation2365213
display id: annotation2365213
version: 1
top level: BBa_K1165003
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1165003/annotation2365213/range2365213/1

https://synbiohub.org/public/igem/BBa_K1165003/annotation2365213/range2365213/1
type: Range
persistent identity: range2365213
display id: range2365213
version: 1
direction: direction/forward
top level: BBa_K1165003
start: 44
end: 69
orientation: inline

Target Binding Arms
type: SequenceAnnotation
persistent identity: annotation2365214
display id: annotation2365214
version: 1
top level: BBa_K1165003
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1165003/annotation2365214/range2365214/1

https://synbiohub.org/public/igem/BBa_K1165003/annotation2365214/range2365214/1
type: Range
persistent identity: range2365214
display id: range2365214
version: 1
direction: direction/forward
top level: BBa_K1165003
start: 70
end: 81
orientation: inline

Synthetic Extended Arms
type: SequenceAnnotation
persistent identity: annotation2365209
display id: annotation2365209
version: 1
top level: BBa_K1165003
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1165003/annotation2365209/range2365209/1

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type: Range
persistent identity: range2365209
display id: range2365209
version: 1
direction: direction/forward
top level: BBa_K1165003
start: 1
end: 30
orientation: inline

Target-Binding Arms
type: SequenceAnnotation
persistent identity: annotation2365210
display id: annotation2365210
version: 1
top level: BBa_K1165003
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1165003/annotation2365210/range2365210/1

https://synbiohub.org/public/igem/BBa_K1165003/annotation2365210/range2365210/1
type: Range
persistent identity: range2365210
display id: range2365210
version: 1
direction: direction/forward
top level: BBa_K1165003
start: 32
end: 35
orientation: inline

Target-Binding Arms
type: SequenceAnnotation
persistent identity: annotation2365212
display id: annotation2365212
version: 1
top level: BBa_K1165003
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1165003/annotation2365212/range2365212/1

https://synbiohub.org/public/igem/BBa_K1165003/annotation2365212/range2365212/1
type: Range
persistent identity: range2365212
display id: range2365212
version: 1
direction: direction/forward
top level: BBa_K1165003
start: 39
end: 43
orientation: inline

TAA Bump
type: SequenceAnnotation
persistent identity: annotation2365211
display id: annotation2365211
version: 1
top level: BBa_K1165003
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1165003/annotation2365211/range2365211/1

https://synbiohub.org/public/igem/BBa_K1165003/annotation2365211/range2365211/1
type: Range
persistent identity: range2365211
display id: range2365211
version: 1
direction: direction/forward
top level: BBa_K1165003
start: 36
end: 38
orientation: inline

Synthetic Extended Arms
type: SequenceAnnotation
persistent identity: annotation2365215
display id: annotation2365215
version: 1
top level: BBa_K1165003
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1165003/annotation2365215/range2365215/1

https://synbiohub.org/public/igem/BBa_K1165003/annotation2365215/range2365215/1
type: Range
persistent identity: range2365215
display id: range2365215
version: 1
direction: direction/forward
top level: BBa_K1165003
start: 82
end: 111
orientation: inline

BBa_K2088010
type: ComponentDefinition
persistent identity: BBa K2088010
display id: BBa_K2088010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: A device that expresses the XylE- catechol 2,3-dioxygenase to degrade the catechol .
creator: Shi Kejian
created: 2016-10-07T11:00:00Z
modified: 2016-10-18T11:21:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2556_
m user id: 33751
owner id: 29893
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Before the XylE - catechol 2,3-dioxygenase, the promoter J23100 and the RBS B0034 are connected. The device can be used to degrade the catechol in some degradation system to decrease the content of the catechol for the catechol can restrain the growth of the bacteria, also, the catechol is a kind of common degradation intermediate product.
mutable notes: We linked the K316003 with the promoter J23100 and the RBS B0034 ahead.
mutable provenance: We use the parts in kit2016 to complete the device.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_J23100
component: BBa_B0034
component: BBa_K316003
sequence annotation: BBa_B0034
sequence annotation: BBa_K316003
sequence annotation: BBa_J23100
sequence: https://synbiohub.org/public/igem/BBa_K2088010_sequence/1

BBa_J23100
type: Component
persistent identity: component2517353
display id: component2517353
version: 1
top level: BBa_K2088010
access: public
definition: https://synbiohub.org/public/igem/BBa_J23100/1

BBa_B0034
type: Component
persistent identity: component2517355
display id: component2517355
version: 1
top level: BBa_K2088010
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K316003
type: Component
persistent identity: component2517366
display id: component2517366
version: 1
top level: BBa_K2088010
access: public
definition: https://synbiohub.org/public/igem/BBa_K316003/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2517355
display id: annotation2517355
version: 1
top level: BBa_K2088010
location: https://synbiohub.org/public/igem/BBa_K2088010/annotation2517355/range2517355/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K2088010/annotation2517355/range2517355/1
type: Range
persistent identity: range2517355
display id: range2517355
version: 1
direction: direction/reverse
top level: BBa_K2088010
start: 44
end: 55
orientation: inline

BBa_K316003
type: SequenceAnnotation
persistent identity: annotation2517366
display id: annotation2517366
version: 1
top level: BBa_K2088010
location: https://synbiohub.org/public/igem/BBa_K2088010/annotation2517366/range2517366/1
component: BBa_K316003

https://synbiohub.org/public/igem/BBa_K2088010/annotation2517366/range2517366/1
type: Range
persistent identity: range2517366
display id: range2517366
version: 1
direction: direction/reverse
top level: BBa_K2088010
start: 64
end: 1124
orientation: inline

BBa_J23100
type: SequenceAnnotation
persistent identity: annotation2517353
display id: annotation2517353
version: 1
top level: BBa_K2088010
location: https://synbiohub.org/public/igem/BBa_K2088010/annotation2517353/range2517353/1
component: BBa_J23100

https://synbiohub.org/public/igem/BBa_K2088010/annotation2517353/range2517353/1
type: Range
persistent identity: range2517353
display id: range2517353
version: 1
direction: direction/reverse
top level: BBa_K2088010
start: 1
end: 35
orientation: inline

BBa_K2010004
type: ComponentDefinition
persistent identity: BBa K2010004
display id: BBa_K2010004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: sfGFP + PETase (PET-degrading enzyme, origin I. sakaiensis)
creator: William Cho
created: 2016-06-20T11:00:00Z
modified: 2016-10-08T01:16:39Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2477_
m user id: 30331
owner id: 30331
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This His-tag is a sequence that allows a conjunct protein to be purified. The tag is composed of repetitions of histidines.
mutable notes: The DNA sequence has been optimized for E. coli K12 and modified to facilitate gBlock synthesis, by avoiding repeated sequences.
mutable provenance: This sequence is publicly known and was privately modified.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: T7 Promoter
sequence annotation: sfGFP
sequence annotation: His-Tag
sequence annotation: Start Codon
sequence annotation: Stop Codon
sequence annotation: PETase
sequence annotation: Linker
sequence annotation: GGS Linker
sequence annotation: RBS 34
sequence: https://synbiohub.org/public/igem/BBa_K2010004_sequence/1

T7 Promoter
type: SequenceAnnotation
persistent identity: annotation2480263
display id: annotation2480263
version: 1
top level: BBa_K2010004
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K2010004/annotation2480263/range2480263/1

https://synbiohub.org/public/igem/BBa_K2010004/annotation2480263/range2480263/1
type: Range
persistent identity: range2480263
display id: range2480263
version: 1
direction: direction/forward
top level: BBa_K2010004
start: 1
end: 46
orientation: inline

sfGFP
type: SequenceAnnotation
persistent identity: annotation2483576
display id: annotation2483576
version: 1
top level: BBa_K2010004
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K2010004/annotation2483576/range2483576/1

https://synbiohub.org/public/igem/BBa_K2010004/annotation2483576/range2483576/1
type: Range
persistent identity: range2483576
display id: range2483576
version: 1
direction: direction/forward
top level: BBa_K2010004
start: 65
end: 778
orientation: inline

His-Tag
type: SequenceAnnotation
persistent identity: annotation2483580
display id: annotation2483580
version: 1
top level: BBa_K2010004
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K2010004/annotation2483580/range2483580/1

https://synbiohub.org/public/igem/BBa_K2010004/annotation2483580/range2483580/1
type: Range
persistent identity: range2483580
display id: range2483580
version: 1
direction: direction/forward
top level: BBa_K2010004
start: 1700
end: 1717
orientation: inline

Start Codon
type: SequenceAnnotation
persistent identity: annotation2483581
display id: annotation2483581
version: 1
top level: BBa_K2010004
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K2010004/annotation2483581/range2483581/1

https://synbiohub.org/public/igem/BBa_K2010004/annotation2483581/range2483581/1
type: Range
persistent identity: range2483581
display id: range2483581
version: 1
direction: direction/forward
top level: BBa_K2010004
start: 65
end: 67
orientation: inline

Stop Codon
type: SequenceAnnotation
persistent identity: annotation2483582
display id: annotation2483582
version: 1
top level: BBa_K2010004
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K2010004/annotation2483582/range2483582/1

https://synbiohub.org/public/igem/BBa_K2010004/annotation2483582/range2483582/1
type: Range
persistent identity: range2483582
display id: range2483582
version: 1
direction: direction/forward
top level: BBa_K2010004
start: 1718
end: 1723
orientation: inline

PETase
type: SequenceAnnotation
persistent identity: annotation2483578
display id: annotation2483578
version: 1
top level: BBa_K2010004
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K2010004/annotation2483578/range2483578/1

https://synbiohub.org/public/igem/BBa_K2010004/annotation2483578/range2483578/1
type: Range
persistent identity: range2483578
display id: range2483578
version: 1
direction: direction/forward
top level: BBa_K2010004
start: 794
end: 1663
orientation: inline

Linker
type: SequenceAnnotation
persistent identity: annotation2483577
display id: annotation2483577
version: 1
top level: BBa_K2010004
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K2010004/annotation2483577/range2483577/1

https://synbiohub.org/public/igem/BBa_K2010004/annotation2483577/range2483577/1
type: Range
persistent identity: range2483577
display id: range2483577
version: 1
direction: direction/forward
top level: BBa_K2010004
start: 779
end: 793
orientation: inline

GGS Linker
type: SequenceAnnotation
persistent identity: annotation2483579
display id: annotation2483579
version: 1
top level: BBa_K2010004
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K2010004/annotation2483579/range2483579/1

https://synbiohub.org/public/igem/BBa_K2010004/annotation2483579/range2483579/1
type: Range
persistent identity: range2483579
display id: range2483579
version: 1
direction: direction/forward
top level: BBa_K2010004
start: 1664
end: 1699
orientation: inline

RBS 34
type: SequenceAnnotation
persistent identity: annotation2483575
display id: annotation2483575
version: 1
top level: BBa_K2010004
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K2010004/annotation2483575/range2483575/1

https://synbiohub.org/public/igem/BBa_K2010004/annotation2483575/range2483575/1
type: Range
persistent identity: range2483575
display id: range2483575
version: 1
direction: direction/forward
top level: BBa_K2010004
start: 47
end: 58
orientation: inline

BBa_K2010000
type: ComponentDefinition
persistent identity: BBa K2010000
display id: BBa_K2010000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PETase (PET-degrading enzyme, origin I. sakaiensis)
creator: William Cho
created: 2016-06-16T11:00:00Z
modified: 2016-10-02T01:25:32Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2477_
m user id: 30331
owner id: 30331
owning group id: 9
status: status/Planning
bookmark: false
mutable description: PETase is the PET-degrading enzyme first identified in Ideonella sakaiensis. This sequence is the E. coli K12 optimized DNA sequence for PETase.
mutable notes: This sequence was optimized for E. coli K12 using the Codon Optimization Tool from the IDT website.
mutable provenance: This part comes from Ideonella sakaiensis, identified in "A bacterium that degrades and assimilates poly(ethylene terephthalate)" (Yoshida et al. 2016).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: His-Tag
sequence annotation: T7 Promoter
sequence annotation: Stop Codon
sequence annotation: GGS Linker
sequence annotation: RBS 34
sequence annotation: Start Codon
sequence annotation: PETase
sequence: https://synbiohub.org/public/igem/BBa_K2010000_sequence/1

His-Tag
type: SequenceAnnotation
persistent identity: annotation2483552
display id: annotation2483552
version: 1
top level: BBa_K2010000
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K2010000/annotation2483552/range2483552/1

https://synbiohub.org/public/igem/BBa_K2010000/annotation2483552/range2483552/1
type: Range
persistent identity: range2483552
display id: range2483552
version: 1
direction: direction/forward
top level: BBa_K2010000
start: 965
end: 982
orientation: inline

T7 Promoter
type: SequenceAnnotation
persistent identity: annotation2480240
display id: annotation2480240
version: 1
top level: BBa_K2010000
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K2010000/annotation2480240/range2480240/1

https://synbiohub.org/public/igem/BBa_K2010000/annotation2480240/range2480240/1
type: Range
persistent identity: range2480240
display id: range2480240
version: 1
direction: direction/forward
top level: BBa_K2010000
start: 1
end: 46
orientation: inline

Stop Codon
type: SequenceAnnotation
persistent identity: annotation2483553
display id: annotation2483553
version: 1
top level: BBa_K2010000
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K2010000/annotation2483553/range2483553/1

https://synbiohub.org/public/igem/BBa_K2010000/annotation2483553/range2483553/1
type: Range
persistent identity: range2483553
display id: range2483553
version: 1
direction: direction/forward
top level: BBa_K2010000
start: 983
end: 988
orientation: inline

GGS Linker
type: SequenceAnnotation
persistent identity: annotation2483551
display id: annotation2483551
version: 1
top level: BBa_K2010000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K2010000/annotation2483551/range2483551/1

https://synbiohub.org/public/igem/BBa_K2010000/annotation2483551/range2483551/1
type: Range
persistent identity: range2483551
display id: range2483551
version: 1
direction: direction/forward
top level: BBa_K2010000
start: 929
end: 964
orientation: inline

RBS 34
type: SequenceAnnotation
persistent identity: annotation2480241
display id: annotation2480241
version: 1
top level: BBa_K2010000
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K2010000/annotation2480241/range2480241/1

https://synbiohub.org/public/igem/BBa_K2010000/annotation2480241/range2480241/1
type: Range
persistent identity: range2480241
display id: range2480241
version: 1
direction: direction/forward
top level: BBa_K2010000
start: 47
end: 58
orientation: inline

Start Codon
type: SequenceAnnotation
persistent identity: annotation2483554
display id: annotation2483554
version: 1
top level: BBa_K2010000
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K2010000/annotation2483554/range2483554/1

https://synbiohub.org/public/igem/BBa_K2010000/annotation2483554/range2483554/1
type: Range
persistent identity: range2483554
display id: range2483554
version: 1
direction: direction/forward
top level: BBa_K2010000
start: 59
end: 61
orientation: inline

PETase
type: SequenceAnnotation
persistent identity: annotation2480239
display id: annotation2480239
version: 1
top level: BBa_K2010000
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K2010000/annotation2480239/range2480239/1

https://synbiohub.org/public/igem/BBa_K2010000/annotation2480239/range2480239/1
type: Range
persistent identity: range2480239
display id: range2480239
version: 1
direction: direction/forward
top level: BBa_K2010000
start: 59
end: 928
orientation: inline

BBa_K174000
type: ComponentDefinition
persistent identity: BBa K174000
display id: BBa_K174000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: SspB proteolysis chaperone
creator: The Newcastle 2009 iGEM team
created: 2009-09-25T11:00:00Z
modified: 2015-05-08T01:10:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _277_
m user id: 0
owner id: 3942
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: SspB protein targets proteins tagged with modified degradation tag, ssrA, and deliver them to ClpXP for proteolysis. Modified ssrA tags are fused onto the 3??? end of a gene.
mutable notes: Forward primer used: GATCTG-GAATTCGCGGCCGCTTCTAG-ATGGATTTGTCACAGCTAAC (Clamp sequence - Standard Biobrick prefix - first 20 base from the Biobrick) Reverse primer used: TGTGAC-ACTAGTA-TTACTTCACAACGCGTAATGC (Clamp sequence - SpeI site - last 21 base from the Biobrick)
mutable provenance: 498 bp long sspB CDS is amplified by PCR with dangling end primers with EcoRI-NotI-XbaI restriction site at 5??? and SpeI at 3??? and inserted into a Biobrick compatible vector. The sequence is taken from E. coli strain DH5 alpha. Genbank accession number for E. coli MG1655 strain is NC_000913.2
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: sspB
sequence: https://synbiohub.org/public/igem/BBa_K174000_sequence/1

sspB
type: SequenceAnnotation
persistent identity: annotation2027215
display id: annotation2027215
version: 1
top level: BBa_K174000
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K174000/annotation2027215/range2027215/1

https://synbiohub.org/public/igem/BBa_K174000/annotation2027215/range2027215/1
type: Range
persistent identity: range2027215
display id: range2027215
version: 1
direction: direction/forward
top level: BBa_K174000
start: 1
end: 498
orientation: inline

Bt-ADH
type: ComponentDefinition
persistent identity: BBa K398005
display id: BBa_K398005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Medium-Chain Alcohol Dehydrogenase
creator: Mathias Voges and Hugo F. Cueto Rojas
created: 2010-06-12T11:00:00Z
modified: 2015-05-08T01:12:22Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _506_
m user id: 0
owner id: 6320
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: Alcohol dehydrogenase from Bacillus thermoleovorans B23.
mutable notes: Nucleotide sequence optimized for expression in E.coli.
mutable provenance: Genbank
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: stop
sequence annotation: Bt-ADH
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_K398005_sequence/1

stop
type: SequenceAnnotation
persistent identity: annotation2070754
display id: annotation2070754
version: 1
top level: Bt-ADH
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K398005/annotation2070754/range2070754/1

https://synbiohub.org/public/igem/BBa_K398005/annotation2070754/range2070754/1
type: Range
persistent identity: range2070754
display id: range2070754
version: 1
direction: direction/forward
top level: Bt-ADH
start: 748
end: 750
orientation: inline

Bt-ADH
type: SequenceAnnotation
persistent identity: annotation2070752
display id: annotation2070752
version: 1
top level: Bt-ADH
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K398005/annotation2070752/range2070752/1

https://synbiohub.org/public/igem/BBa_K398005/annotation2070752/range2070752/1
type: Range
persistent identity: range2070752
display id: range2070752
version: 1
direction: direction/forward
top level: Bt-ADH
start: 1
end: 750
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2070753
display id: annotation2070753
version: 1
top level: Bt-ADH
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K398005/annotation2070753/range2070753/1

https://synbiohub.org/public/igem/BBa_K398005/annotation2070753/range2070753/1
type: Range
persistent identity: range2070753
display id: range2070753
version: 1
direction: direction/forward
top level: Bt-ADH
start: 1
end: 3
orientation: inline

BBa_K1398001
type: ComponentDefinition
persistent identity: BBa K1398001
display id: BBa_K1398001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: XenB (Inducible Construct)
creator: Exeter iGEM 2014
created: 2014-08-10T11:00:00Z
modified: 2015-05-08T01:10:15Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1776_
m user id: 0
owner id: 19951
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: A construct created to degraded TNT and nitroglycerine. The construct contains the coding sequence for XenB (BBa_K1398000), an enzyme with the capability to degrade nitro- groups in chemicals. The construct also contains an inducible promoter (BBa_R0010), a strong RBS (BBa_B0034) and a double terminator (BBa_B0010). The protein has been codon-optimised for expression in E. coli.
mutable notes: This construct was created to allow for the over-expression of XenB, so it contains high expression regulatory sequences. A lactose inducible promoter has been used to give control over expression.
mutable provenance: The original source of XenB was Pseudomonas putida. The regulatory sequences were acquired from the iGEM repository.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0015
component: BBa_R0010
component: BBa_K1398000
component: BBa_B0034
sequence annotation: BBa_B0015
sequence annotation: BBa_R0010
sequence annotation: BBa_K1398000
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K1398001_sequence/1

BBa_B0015
type: Component
persistent identity: component2380401
display id: component2380401
version: 1
top level: BBa_K1398001
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_R0010
type: Component
persistent identity: component2380385
display id: component2380385
version: 1
top level: BBa_K1398001
access: public
definition: https://synbiohub.org/public/igem/BBa_R0010/1

BBa_K1398000
type: Component
persistent identity: component2380394
display id: component2380394
version: 1
top level: BBa_K1398001
access: public
definition: XenB

BBa_B0034
type: Component
persistent identity: component2380393
display id: component2380393
version: 1
top level: BBa_K1398001
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2380401
display id: annotation2380401
version: 1
top level: BBa_K1398001
location: https://synbiohub.org/public/igem/BBa_K1398001/annotation2380401/range2380401/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K1398001/annotation2380401/range2380401/1
type: Range
persistent identity: range2380401
display id: range2380401
version: 1
direction: direction/reverse
top level: BBa_K1398001
start: 1300
end: 1428
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation2380385
display id: annotation2380385
version: 1
top level: BBa_K1398001
location: https://synbiohub.org/public/igem/BBa_K1398001/annotation2380385/range2380385/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_K1398001/annotation2380385/range2380385/1
type: Range
persistent identity: range2380385
display id: range2380385
version: 1
direction: direction/forward
top level: BBa_K1398001
start: 1
end: 200
orientation: inline

BBa_K1398000
type: SequenceAnnotation
persistent identity: annotation2380394
display id: annotation2380394
version: 1
top level: BBa_K1398001
location: https://synbiohub.org/public/igem/BBa_K1398001/annotation2380394/range2380394/1
component: BBa_K1398000

https://synbiohub.org/public/igem/BBa_K1398001/annotation2380394/range2380394/1
type: Range
persistent identity: range2380394
display id: range2380394
version: 1
direction: direction/reverse
top level: BBa_K1398001
start: 227
end: 1291
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2380393
display id: annotation2380393
version: 1
top level: BBa_K1398001
location: https://synbiohub.org/public/igem/BBa_K1398001/annotation2380393/range2380393/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1398001/annotation2380393/range2380393/1
type: Range
persistent identity: range2380393
display id: range2380393
version: 1
direction: direction/reverse
top level: BBa_K1398001
start: 209
end: 220
orientation: inline

BBa_K078005
type: ComponentDefinition
persistent identity: BBa K078005
display id: BBa_K078005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: 2，2???，3-Trihydroxybiphenyl 1,2-dioxygenase. The second step enzyme in dixon degradati
creator: Sally.Wang (Jingyi Wang)
created: 2008-10-14T11:00:00Z
modified: 2015-05-08T01:08:33Z
discontinued: false
dominant: false
group u list: _184_
m user id: 0
owner id: 2670
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: 2，2???，3-Trihydroxybiphenyl 1,2-dioxygenase.The second step meta cleavage enzyme in dioxin degradation pathway. According to the standard, we add EcoR1,Not1 and Xba1 upstream; and spe1, Not1, Pst1 and stop condon downstream. Anyone of the five sites could be used except Not1. The vector is the standard vector provided by biobrick08. We recommend double digestion of EcoRI+SpeI.Digest in NEBuffer EcoRI + BSA at 37??C for about 3h.
mutable notes: We use it to construct the degradation pathway of dioxin. According to the standard, we add EcoR1,Not1 and Xba1 upstream; and spe1, Not1, Pst1 and stop condon downstream. Anyone of the five sites could be used except Not1. The vector is the standard vector provided by biobrick08. We recommend double digestion of EcoRI+SpeI.Digest in NEBuffer EcoRI + BSA at 37??C for about 3h.
mutable provenance: from the dibenzofuran- and dibenzo-p-dioxin-degrading bacterium Sphingomonas sp. strain RW1. Type: genomic DNA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: dbfB
sequence: https://synbiohub.org/public/igem/BBa_K078005_sequence/1

dbfB
type: SequenceAnnotation
persistent identity: annotation1983863
display id: annotation1983863
version: 1
top level: BBa_K078005
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K078005/annotation1983863/range1983863/1

https://synbiohub.org/public/igem/BBa_K078005/annotation1983863/range1983863/1
type: Range
persistent identity: range1983863
display id: range1983863
version: 1
direction: direction/forward
top level: BBa_K078005
start: 1
end: 885
orientation: inline

BBa_K078102
type: ComponentDefinition
persistent identity: BBa K078102
display id: BBa_K078102
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: dxnB
creator: Sally.Wang (Jingyi Wang)
created: 2008-10-23T11:00:00Z
modified: 2015-05-08T01:08:33Z
discontinued: false
dominant: false
group u list: _184_
m user id: 0
owner id: 2670
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: We use this part to construct the degradation pathway of dioxin. This is a standard parts. Acoording to the standard, we add EcoR1,Not1 and Xba1 upstream; and spe1, Not1, Pst1 and stop condon downstream. Anyone of the five sites could be used . The vector is the standard vector,pSB1AC3, provided by biobrick08. We recommend double digestion of EcoRI+PstI.Digest in NEBuffer EcoRI + BSA at 37??C for about 3h.
mutable notes: We use this part to construct the degradation pathway of dioxin. This is a standard parts. Acoording to the standard, we add EcoR1,Not1 and Xba1 upstream; and spe1, Not1, Pst1 and stop condon downstream. Anyone of the five sites could be used . The vector is the standard vector,pSB1AC3, provided by biobrick08. We recommend double digestion of EcoRI+PstI.Digest in NEBuffer EcoRI + BSA at 37??C for about 3h.
mutable provenance: an extradiol dioxygenase from the dibenzofuran- and dibenzo-p-dioxin-degrading bacterium Sphingomonas sp. strain RW1. Type: genomic DNA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K078102_sequence/1

BBa_K863000
type: ComponentDefinition
persistent identity: BBa K863000
display id: BBa_K863000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: bpul (laccase from Bacillus pumilus) with T7 promoter, RBS and HIS tag
creator: Isabel Huber
created: 2012-09-17T11:00:00Z
modified: 2015-05-08T01:13:36Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1123_
m user id: 0
owner id: 12844
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: bpul (Laccase from Bacillus pumilus) with T7, RBS and HIS tag
mutable notes: test
mutable provenance: Bacillus pumilus DSM 27 (ATCC7061)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: A (Original) T (Mutation)
sequence annotation: TAA
sequence annotation: RBS
sequence annotation: 6 x HIS
sequence annotation: bpul
sequence annotation: T7- Promotor BBa_I719005
sequence annotation: ATG
sequence: https://synbiohub.org/public/igem/BBa_K863000_sequence/1

A (Original) T (Mutation)
type: SequenceAnnotation
persistent identity: annotation2184912
display id: annotation2184912
version: 1
top level: BBa_K863000
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_K863000/annotation2184912/range2184912/1

https://synbiohub.org/public/igem/BBa_K863000/annotation2184912/range2184912/1
type: Range
persistent identity: range2184912
display id: range2184912
version: 1
direction: direction/forward
top level: BBa_K863000
start: 834
end: 834
orientation: inline

TAA
type: SequenceAnnotation
persistent identity: annotation2184915
display id: annotation2184915
version: 1
top level: BBa_K863000
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K863000/annotation2184915/range2184915/1

https://synbiohub.org/public/igem/BBa_K863000/annotation2184915/range2184915/1
type: Range
persistent identity: range2184915
display id: range2184915
version: 1
direction: direction/forward
top level: BBa_K863000
start: 1585
end: 1587
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2184909
display id: annotation2184909
version: 1
top level: BBa_K863000
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K863000/annotation2184909/range2184909/1

https://synbiohub.org/public/igem/BBa_K863000/annotation2184909/range2184909/1
type: Range
persistent identity: range2184909
display id: range2184909
version: 1
direction: direction/forward
top level: BBa_K863000
start: 25
end: 30
orientation: inline

6 x HIS
type: SequenceAnnotation
persistent identity: annotation2184913
display id: annotation2184913
version: 1
top level: BBa_K863000
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K863000/annotation2184913/range2184913/1

https://synbiohub.org/public/igem/BBa_K863000/annotation2184913/range2184913/1
type: Range
persistent identity: range2184913
display id: range2184913
version: 1
direction: direction/forward
top level: BBa_K863000
start: 1557
end: 1581
orientation: inline

bpul
type: SequenceAnnotation
persistent identity: annotation2184911
display id: annotation2184911
version: 1
top level: BBa_K863000
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K863000/annotation2184911/range2184911/1

https://synbiohub.org/public/igem/BBa_K863000/annotation2184911/range2184911/1
type: Range
persistent identity: range2184911
display id: range2184911
version: 1
direction: direction/forward
top level: BBa_K863000
start: 34
end: 1556
orientation: inline

T7- Promotor BBa_I719005
type: SequenceAnnotation
persistent identity: annotation2184908
display id: annotation2184908
version: 1
top level: BBa_K863000
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K863000/annotation2184908/range2184908/1

https://synbiohub.org/public/igem/BBa_K863000/annotation2184908/range2184908/1
type: Range
persistent identity: range2184908
display id: range2184908
version: 1
direction: direction/forward
top level: BBa_K863000
start: 1
end: 23
orientation: inline

ATG
type: SequenceAnnotation
persistent identity: annotation2184910
display id: annotation2184910
version: 1
top level: BBa_K863000
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K863000/annotation2184910/range2184910/1

https://synbiohub.org/public/igem/BBa_K863000/annotation2184910/range2184910/1
type: Range
persistent identity: range2184910
display id: range2184910
version: 1
direction: direction/forward
top level: BBa_K863000
start: 34
end: 36
orientation: inline

TAA
type: SequenceAnnotation
persistent identity: annotation2184914
display id: annotation2184914
version: 1
top level: BBa_K863000
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K863000/annotation2184914/range2184914/1

https://synbiohub.org/public/igem/BBa_K863000/annotation2184914/range2184914/1
type: Range
persistent identity: range2184914
display id: range2184914
version: 1
direction: direction/forward
top level: BBa_K863000
start: 1582
end: 1584
orientation: inline

BBa_K078003
type: ComponentDefinition
persistent identity: BBa K078003
display id: BBa_K078003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: one part of the initial dioxygenase of the dioxin degradation pathway
creator: Sally.Wang (Jingyi Wang)
created: 2008-10-13T11:00:00Z
modified: 2015-05-08T01:08:33Z
discontinued: false
dominant: false
experience: experience/None
group u list: _184_
m user id: 0
owner id: 2670
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: The initial dioxygenase of the dioxin degradation pathway is a three-component enzyme with an atypical electron supply system.dxnA is one part of this muticomponent complex which acts as ring hydroxylase.dxnA harbors two subunits,dxnA1 and dxnA2. This is a standard parts. Acoording to the standard, we add EcoR1,Not1 and Xba1 upstream; and spe1, Not1, Pst1 and stop condon downstream. Anyone of the five sites could be used except pst1. The vector is the standard vector provided by biobrick08. We recommend double digestion of EcoRI+SpeI.Digest in NEBuffer EcoRI + BSA at 37??C for about 3h.
mutable notes: We use this part to construct the degradation pathway of dioxin.
mutable provenance: an extradiol dioxygenase from the dibenzofuran- and dibenzo-p-dioxin-degrading bacterium Sphingomonas sp. strain RW1. Type: genomic DNA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K078003_sequence/1

BBa_K174002
type: ComponentDefinition
persistent identity: BBa K174002
display id: BBa_K174002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Arabinose controlled protein degradation device
creator: The Newcastle 2009 iGEM team
created: 2009-09-25T11:00:00Z
modified: 2015-05-08T01:10:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _277_
m user id: 0
owner id: 3942
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: The purpose of this system is to control the expression of sspB, the degradation controller. The system is inducible by arabinose, therefore we can alter the arabinose levels to regulate the degradation of proteins with sspB degradation tag
mutable notes: sspB Biobrick is inserted after araR promoter+rbs system
mutable provenance: Comprised of other Biobricks
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K174000
component: BBa_K174001
sequence annotation: BBa_K174001
sequence annotation: BBa_K174000
sequence: https://synbiohub.org/public/igem/BBa_K174002_sequence/1

BBa_K174000
type: Component
persistent identity: component2027246
display id: component2027246
version: 1
top level: BBa_K174002
access: public
definition: BBa_K174000

BBa_K174001
type: Component
persistent identity: component2027244
display id: component2027244
version: 1
top level: BBa_K174002
access: public
definition: https://synbiohub.org/public/igem/BBa_K174001/1

BBa_K174001
type: SequenceAnnotation
persistent identity: annotation2027244
display id: annotation2027244
version: 1
top level: BBa_K174002
location: https://synbiohub.org/public/igem/BBa_K174002/annotation2027244/range2027244/1
component: BBa_K174001

https://synbiohub.org/public/igem/BBa_K174002/annotation2027244/range2027244/1
type: Range
persistent identity: range2027244
display id: range2027244
version: 1
direction: direction/reverse
top level: BBa_K174002
start: 1
end: 215
orientation: inline

BBa_K174000
type: SequenceAnnotation
persistent identity: annotation2027246
display id: annotation2027246
version: 1
top level: BBa_K174002
location: https://synbiohub.org/public/igem/BBa_K174002/annotation2027246/range2027246/1
component: BBa_K174000

https://synbiohub.org/public/igem/BBa_K174002/annotation2027246/range2027246/1
type: Range
persistent identity: range2027246
display id: range2027246
version: 1
direction: direction/reverse
top level: BBa_K174002
start: 222
end: 719
orientation: inline

BBa_K2010008
type: ComponentDefinition
persistent identity: BBa K2010008
display id: BBa_K2010008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: YebF + sfGFP + PETase
creator: William Cho
created: 2016-09-15T11:00:00Z
modified: 2016-10-08T01:33:49Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2477_
m user id: 30331
owner id: 30331
owning group id: 9
status: status/Planning
bookmark: false
mutable description: YebF + sfGFP + PETase
mutable notes: YebF + sfGFP + PETase
mutable provenance: YebF + sfGFP + PETase
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: YebF
sequence annotation: PETase
sequence annotation: His-Tag
sequence annotation: RBS 34
sequence annotation: Linker
sequence annotation: sfGFP
sequence annotation: Start Codon
sequence annotation: GGS Linker
sequence annotation: T7 Promoter
sequence annotation: Stop Codon
sequence: https://synbiohub.org/public/igem/BBa_K2010008_sequence/1

YebF
type: SequenceAnnotation
persistent identity: annotation2483619
display id: annotation2483619
version: 1
top level: BBa_K2010008
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K2010008/annotation2483619/range2483619/1

https://synbiohub.org/public/igem/BBa_K2010008/annotation2483619/range2483619/1
type: Range
persistent identity: range2483619
display id: range2483619
version: 1
direction: direction/forward
top level: BBa_K2010008
start: 785
end: 1138
orientation: inline

PETase
type: SequenceAnnotation
persistent identity: annotation2483621
display id: annotation2483621
version: 1
top level: BBa_K2010008
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K2010008/annotation2483621/range2483621/1

https://synbiohub.org/public/igem/BBa_K2010008/annotation2483621/range2483621/1
type: Range
persistent identity: range2483621
display id: range2483621
version: 1
direction: direction/forward
top level: BBa_K2010008
start: 1154
end: 2023
orientation: inline

His-Tag
type: SequenceAnnotation
persistent identity: annotation2483623
display id: annotation2483623
version: 1
top level: BBa_K2010008
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K2010008/annotation2483623/range2483623/1

https://synbiohub.org/public/igem/BBa_K2010008/annotation2483623/range2483623/1
type: Range
persistent identity: range2483623
display id: range2483623
version: 1
direction: direction/forward
top level: BBa_K2010008
start: 2060
end: 2077
orientation: inline

RBS 34
type: SequenceAnnotation
persistent identity: annotation2483615
display id: annotation2483615
version: 1
top level: BBa_K2010008
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K2010008/annotation2483615/range2483615/1

https://synbiohub.org/public/igem/BBa_K2010008/annotation2483615/range2483615/1
type: Range
persistent identity: range2483615
display id: range2483615
version: 1
direction: direction/forward
top level: BBa_K2010008
start: 47
end: 58
orientation: inline

Linker
type: SequenceAnnotation
persistent identity: annotation2483618
display id: annotation2483618
version: 1
top level: BBa_K2010008
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K2010008/annotation2483618/range2483618/1

https://synbiohub.org/public/igem/BBa_K2010008/annotation2483618/range2483618/1
type: Range
persistent identity: range2483618
display id: range2483618
version: 1
direction: direction/forward
top level: BBa_K2010008
start: 779
end: 784
orientation: inline

sfGFP
type: SequenceAnnotation
persistent identity: annotation2483617
display id: annotation2483617
version: 1
top level: BBa_K2010008
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K2010008/annotation2483617/range2483617/1

https://synbiohub.org/public/igem/BBa_K2010008/annotation2483617/range2483617/1
type: Range
persistent identity: range2483617
display id: range2483617
version: 1
direction: direction/forward
top level: BBa_K2010008
start: 65
end: 778
orientation: inline

Start Codon
type: SequenceAnnotation
persistent identity: annotation2483625
display id: annotation2483625
version: 1
top level: BBa_K2010008
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K2010008/annotation2483625/range2483625/1

https://synbiohub.org/public/igem/BBa_K2010008/annotation2483625/range2483625/1
type: Range
persistent identity: range2483625
display id: range2483625
version: 1
direction: direction/forward
top level: BBa_K2010008
start: 65
end: 67
orientation: inline

GGS Linker
type: SequenceAnnotation
persistent identity: annotation2483622
display id: annotation2483622
version: 1
top level: BBa_K2010008
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K2010008/annotation2483622/range2483622/1

https://synbiohub.org/public/igem/BBa_K2010008/annotation2483622/range2483622/1
type: Range
persistent identity: range2483622
display id: range2483622
version: 1
direction: direction/forward
top level: BBa_K2010008
start: 2024
end: 2059
orientation: inline

Linker
type: SequenceAnnotation
persistent identity: annotation2483620
display id: annotation2483620
version: 1
top level: BBa_K2010008
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K2010008/annotation2483620/range2483620/1

https://synbiohub.org/public/igem/BBa_K2010008/annotation2483620/range2483620/1
type: Range
persistent identity: range2483620
display id: range2483620
version: 1
direction: direction/forward
top level: BBa_K2010008
start: 1139
end: 1153
orientation: inline

T7 Promoter
type: SequenceAnnotation
persistent identity: annotation2483616
display id: annotation2483616
version: 1
top level: BBa_K2010008
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K2010008/annotation2483616/range2483616/1

https://synbiohub.org/public/igem/BBa_K2010008/annotation2483616/range2483616/1
type: Range
persistent identity: range2483616
display id: range2483616
version: 1
direction: direction/forward
top level: BBa_K2010008
start: 1
end: 46
orientation: inline

Stop Codon
type: SequenceAnnotation
persistent identity: annotation2483624
display id: annotation2483624
version: 1
top level: BBa_K2010008
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K2010008/annotation2483624/range2483624/1

https://synbiohub.org/public/igem/BBa_K2010008/annotation2483624/range2483624/1
type: Range
persistent identity: range2483624
display id: range2483624
version: 1
direction: direction/forward
top level: BBa_K2010008
start: 2078
end: 2083
orientation: inline

BBa_K078009
type: ComponentDefinition
persistent identity: BBa K078009
display id: BBa_K078009
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: bphD codes 2-hydroxy-6-oxo-6-phenylhexa-2,4- dienoate hydrolase
creator: Sally.Wang (Jingyi Wang)
created: 2008-10-21T11:00:00Z
modified: 2015-05-08T01:08:33Z
discontinued: false
dominant: false
group u list: _184_
m user id: 0
owner id: 2670
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: bphD codes 2-hydroxy-6-oxo-6-phenylhexa-2,4- dienoate hydrolase, which is the forth step enzyme of PCBs degradation pathway. This is a standard parts. Acoording to the standard, we add EcoR1,Not1 and Xba1 upstream; and spe1, Not1, Pst1 and stop condon downstream. Anyone of the five sites could be used except EcoR1. The vector is the standard vector, pSB1AC3, provided by biobrick08. We recommend double digestion of xbaI+speI.Digest in NEB Buffer 2 + BSA at 37??C for about 3h.
mutable notes: We use this part to construct the degradation pathway of PCBs This is a standard parts. Acoording to the standard, we add EcoR1,Not1 and Xba1 upstream; and spe1, Not1, Pst1 and stop condon downstream. Anyone of the five sites could be used except EcoR1. The vector is the standard vector, pSB1AC3, provided by biobrick08. We recommend double digestion of xbaI+speI.Digest in NEB Buffer 2 + BSA at 37??C for about 3h.
mutable provenance: From the PCBs-degrading bacterium Burkholderia xenovorans LB400 chromosome 3. Type: genomic DNA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: bphD
sequence: https://synbiohub.org/public/igem/BBa_K078009_sequence/1

bphD
type: SequenceAnnotation
persistent identity: annotation1983860
display id: annotation1983860
version: 1
top level: BBa_K078009
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K078009/annotation1983860/range1983860/1

https://synbiohub.org/public/igem/BBa_K078009/annotation1983860/range1983860/1
type: Range
persistent identity: range1983860
display id: range1983860
version: 1
direction: direction/forward
top level: BBa_K078009
start: 1
end: 860
orientation: inline

BBa_K1031923
type: ComponentDefinition
persistent identity: BBa K1031923
display id: BBa_K1031923
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: J23109-XylB-Terminator
creator: He Shuaixin
created: 2013-10-21T11:00:00Z
modified: 2015-05-08T01:08:48Z
discontinued: false
dominant: false
group u list: _1338_
m user id: 0
owner id: 15843
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: J23109-XylB-Terminator
mutable notes: molecular cloning
mutable provenance: Pseudomonas.putida
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: B0015
sequence annotation: J23109
sequence annotation: XylB
sequence: https://synbiohub.org/public/igem/BBa_K1031923_sequence/1

B0015
type: SequenceAnnotation
persistent identity: annotation2370041
display id: annotation2370041
version: 1
top level: BBa_K1031923
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1031923/annotation2370041/range2370041/1

https://synbiohub.org/public/igem/BBa_K1031923/annotation2370041/range2370041/1
type: Range
persistent identity: range2370041
display id: range2370041
version: 1
direction: direction/forward
top level: BBa_K1031923
start: 1176
end: 1304
orientation: inline

J23109
type: SequenceAnnotation
persistent identity: annotation2370039
display id: annotation2370039
version: 1
top level: BBa_K1031923
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1031923/annotation2370039/range2370039/1

https://synbiohub.org/public/igem/BBa_K1031923/annotation2370039/range2370039/1
type: Range
persistent identity: range2370039
display id: range2370039
version: 1
direction: direction/forward
top level: BBa_K1031923
start: 1
end: 35
orientation: inline

XylB
type: SequenceAnnotation
persistent identity: annotation2370040
display id: annotation2370040
version: 1
top level: BBa_K1031923
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1031923/annotation2370040/range2370040/1

https://synbiohub.org/public/igem/BBa_K1031923/annotation2370040/range2370040/1
type: Range
persistent identity: range2370040
display id: range2370040
version: 1
direction: direction/forward
top level: BBa_K1031923
start: 63
end: 1169
orientation: inline

BBa_K137000
type: ComponentDefinition
persistent identity: BBa K137000
display id: BBa_K137000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: katE
creator: Doug Tischer
created: 2008-06-19T11:00:00Z
modified: 2015-05-08T01:10:08Z
discontinued: false
dominant: false
experience: experience/None
group u list: _187_
m user id: 0
owner id: 3049
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: E. coli catalase
mutable notes: Site directed mutagenesis to remove internal EcoRI site
mutable provenance: E. coli genomic PCR
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: C-->T
sequence: https://synbiohub.org/public/igem/BBa_K137000_sequence/1

C-->T
type: SequenceAnnotation
persistent identity: annotation1963755
display id: annotation1963755
version: 1
top level: BBa_K137000
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K137000/annotation1963755/range1963755/1

https://synbiohub.org/public/igem/BBa_K137000/annotation1963755/range1963755/1
type: Range
persistent identity: range1963755
display id: range1963755
version: 1
direction: direction/forward
top level: BBa_K137000
start: 1041
end: 1041
orientation: inline

BBa_K863010
type: ComponentDefinition
persistent identity: BBa K863010
display id: BBa_K863010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: tthl laccase from Thermus thermophilus with T7 promoter, RBS and His-tag
creator: Isabel Huber
created: 2012-09-17T11:00:00Z
modified: 2015-05-08T01:13:36Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1123_
m user id: 0
owner id: 12844
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: tthl (Laccase from Thermus thermophilus) with T7, RBS and HIS tag
mutable notes: test
mutable provenance: Thermus thermophilus HB27
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: tthl
sequence annotation: T7 promoter
sequence annotation: 6x HIS
sequence annotation: ATG
sequence annotation: TAA
sequence: https://synbiohub.org/public/igem/BBa_K863010_sequence/1

tthl
type: SequenceAnnotation
persistent identity: annotation2184732
display id: annotation2184732
version: 1
top level: BBa_K863010
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K863010/annotation2184732/range2184732/1

https://synbiohub.org/public/igem/BBa_K863010/annotation2184732/range2184732/1
type: Range
persistent identity: range2184732
display id: range2184732
version: 1
direction: direction/forward
top level: BBa_K863010
start: 34
end: 1419
orientation: inline

T7 promoter
type: SequenceAnnotation
persistent identity: annotation2184708
display id: annotation2184708
version: 1
top level: BBa_K863010
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K863010/annotation2184708/range2184708/1

https://synbiohub.org/public/igem/BBa_K863010/annotation2184708/range2184708/1
type: Range
persistent identity: range2184708
display id: range2184708
version: 1
direction: direction/forward
top level: BBa_K863010
start: 1
end: 23
orientation: inline

6x HIS
type: SequenceAnnotation
persistent identity: annotation2184733
display id: annotation2184733
version: 1
top level: BBa_K863010
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K863010/annotation2184733/range2184733/1

https://synbiohub.org/public/igem/BBa_K863010/annotation2184733/range2184733/1
type: Range
persistent identity: range2184733
display id: range2184733
version: 1
direction: direction/forward
top level: BBa_K863010
start: 1420
end: 1437
orientation: inline

ATG
type: SequenceAnnotation
persistent identity: annotation2184671
display id: annotation2184671
version: 1
top level: BBa_K863010
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K863010/annotation2184671/range2184671/1

https://synbiohub.org/public/igem/BBa_K863010/annotation2184671/range2184671/1
type: Range
persistent identity: range2184671
display id: range2184671
version: 1
direction: direction/forward
top level: BBa_K863010
start: 34
end: 36
orientation: inline

TAA
type: SequenceAnnotation
persistent identity: annotation2184783
display id: annotation2184783
version: 1
top level: BBa_K863010
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K863010/annotation2184783/range2184783/1

https://synbiohub.org/public/igem/BBa_K863010/annotation2184783/range2184783/1
type: Range
persistent identity: range2184783
display id: range2184783
version: 1
direction: direction/forward
top level: BBa_K863010
start: 1441
end: 1443
orientation: inline

TAA
type: SequenceAnnotation
persistent identity: annotation2184782
display id: annotation2184782
version: 1
top level: BBa_K863010
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K863010/annotation2184782/range2184782/1

https://synbiohub.org/public/igem/BBa_K863010/annotation2184782/range2184782/1
type: Range
persistent identity: range2184782
display id: range2184782
version: 1
direction: direction/forward
top level: BBa_K863010
start: 1438
end: 1440
orientation: inline

BBa_K1031625
type: ComponentDefinition
persistent identity: BBa K1031625
display id: BBa_K1031625
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: J23117-NahF-Terminator
creator: He Shuaixin
created: 2013-09-08T11:00:00Z
modified: 2015-05-08T01:08:47Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1338_
m user id: 0
owner id: 15843
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: 117-NahF-TT
mutable notes: molecular cloning
mutable provenance: synthesis Pseudomonas
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: J23117
sequence annotation: NahF
sequence annotation: B0015
sequence: https://synbiohub.org/public/igem/BBa_K1031625_sequence/1

J23117
type: SequenceAnnotation
persistent identity: annotation2361913
display id: annotation2361913
version: 1
top level: BBa_K1031625
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1031625/annotation2361913/range2361913/1

https://synbiohub.org/public/igem/BBa_K1031625/annotation2361913/range2361913/1
type: Range
persistent identity: range2361913
display id: range2361913
version: 1
direction: direction/forward
top level: BBa_K1031625
start: 1
end: 35
orientation: inline

NahF
type: SequenceAnnotation
persistent identity: annotation2361915
display id: annotation2361915
version: 1
top level: BBa_K1031625
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1031625/annotation2361915/range2361915/1

https://synbiohub.org/public/igem/BBa_K1031625/annotation2361915/range2361915/1
type: Range
persistent identity: range2361915
display id: range2361915
version: 1
direction: direction/forward
top level: BBa_K1031625
start: 62
end: 1513
orientation: inline

B0015
type: SequenceAnnotation
persistent identity: annotation2361916
display id: annotation2361916
version: 1
top level: BBa_K1031625
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1031625/annotation2361916/range2361916/1

https://synbiohub.org/public/igem/BBa_K1031625/annotation2361916/range2361916/1
type: Range
persistent identity: range2361916
display id: range2361916
version: 1
direction: direction/forward
top level: BBa_K1031625
start: 1520
end: 1648
orientation: inline

tphB
type: ComponentDefinition
persistent identity: BBa K808010
display id: BBa_K808010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: tphB: reaction from DCD to protocatechuate
creator: Sascha Hein, Sven Rumpf, Daniel Sachs
created: 2012-09-01T11:00:00Z
modified: 2015-05-08T01:13:25Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1065_
m user id: 0
owner id: 11792
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: -
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: protein
sequence: https://synbiohub.org/public/igem/BBa_K808010_sequence/1

protein
type: SequenceAnnotation
persistent identity: annotation2181625
display id: annotation2181625
version: 1
top level: tphB
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K808010/annotation2181625/range2181625/1

https://synbiohub.org/public/igem/BBa_K808010/annotation2181625/range2181625/1
type: Range
persistent identity: range2181625
display id: range2181625
version: 1
direction: direction/forward
top level: tphB
start: 1
end: 948
orientation: inline

XenB
type: ComponentDefinition
persistent identity: BBa K1398000
display id: BBa_K1398000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: XenB (Xenobiotic Reductase B)
creator: Exeter iGEM 2014
created: 2014-08-06T11:00:00Z
modified: 2015-05-08T01:10:15Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1776_
m user id: 0
owner id: 19951
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Xenobiotic Reductase B, created for use as a Trinitrotoluene and Nitroglycerine degrading protein. A monomeric flavin that can reduce certain nitro- groups to nitrate-. Increases the resistance of organisms to the toxic effects of nitrocompounds. This sequence encodes for a protein (with an attached His (x6) Tag to allow for purification) and nothing else. It therefore requires regulatory sequences (promoter/RBS/terminator) to be added for expression.
mutable notes: The original sequence of this part was found using the NCBI database and taken from Pseudomonas putida BIRD-1. The sequence was then codon-optimised for increased expression in E. coli. This has resulted in a novel synthetic sequence.
mutable provenance: Source of original sequence: Pseudomonas putida BIRD-1.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1398000_sequence/1

BBa_K1031922
type: ComponentDefinition
persistent identity: BBa K1031922
display id: BBa_K1031922
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: J23114-XylB-Terminator
creator: He Shuaixin
created: 2013-10-21T11:00:00Z
modified: 2015-05-08T01:08:48Z
discontinued: false
dominant: false
group u list: _1338_
m user id: 0
owner id: 15843
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: J23114-XylB-Terminator
mutable notes: molecular cloning
mutable provenance: Pseudomonas.putida
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: J23114
sequence annotation: B0015
sequence annotation: XylB
sequence: https://synbiohub.org/public/igem/BBa_K1031922_sequence/1

J23114
type: SequenceAnnotation
persistent identity: annotation2370036
display id: annotation2370036
version: 1
top level: BBa_K1031922
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1031922/annotation2370036/range2370036/1

https://synbiohub.org/public/igem/BBa_K1031922/annotation2370036/range2370036/1
type: Range
persistent identity: range2370036
display id: range2370036
version: 1
direction: direction/forward
top level: BBa_K1031922
start: 1
end: 35
orientation: inline

B0015
type: SequenceAnnotation
persistent identity: annotation2370038
display id: annotation2370038
version: 1
top level: BBa_K1031922
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1031922/annotation2370038/range2370038/1

https://synbiohub.org/public/igem/BBa_K1031922/annotation2370038/range2370038/1
type: Range
persistent identity: range2370038
display id: range2370038
version: 1
direction: direction/forward
top level: BBa_K1031922
start: 1176
end: 1304
orientation: inline

XylB
type: SequenceAnnotation
persistent identity: annotation2370037
display id: annotation2370037
version: 1
top level: BBa_K1031922
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1031922/annotation2370037/range2370037/1

https://synbiohub.org/public/igem/BBa_K1031922/annotation2370037/range2370037/1
type: Range
persistent identity: range2370037
display id: range2370037
version: 1
direction: direction/forward
top level: BBa_K1031922
start: 63
end: 1169
orientation: inline

BBa_K863001
type: ComponentDefinition
persistent identity: BBa K863001
display id: BBa_K863001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: bpul laccase from Bacillus pumilus
creator: Isabel Huber
created: 2012-09-17T11:00:00Z
modified: 2015-05-08T01:13:36Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1123_
m user id: 0
owner id: 12844
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: bpul (Laccase from Bacillus pumilus)
mutable notes: test
mutable provenance: Bacillus pumilus DSM 27 (ATCC7061)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: TAA
sequence annotation: A (Original) - T (Mutation)
sequence annotation: ATG
sequence annotation: bpul
sequence: https://synbiohub.org/public/igem/BBa_K863001_sequence/1

TAA
type: SequenceAnnotation
persistent identity: annotation2184641
display id: annotation2184641
version: 1
top level: BBa_K863001
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K863001/annotation2184641/range2184641/1

https://synbiohub.org/public/igem/BBa_K863001/annotation2184641/range2184641/1
type: Range
persistent identity: range2184641
display id: range2184641
version: 1
direction: direction/forward
top level: BBa_K863001
start: 1531
end: 1533
orientation: inline

A (Original) - T (Mutation)
type: SequenceAnnotation
persistent identity: annotation2184905
display id: annotation2184905
version: 1
top level: BBa_K863001
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_K863001/annotation2184905/range2184905/1

https://synbiohub.org/public/igem/BBa_K863001/annotation2184905/range2184905/1
type: Range
persistent identity: range2184905
display id: range2184905
version: 1
direction: direction/forward
top level: BBa_K863001
start: 801
end: 801
orientation: inline

ATG
type: SequenceAnnotation
persistent identity: annotation2184622
display id: annotation2184622
version: 1
top level: BBa_K863001
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K863001/annotation2184622/range2184622/1

https://synbiohub.org/public/igem/BBa_K863001/annotation2184622/range2184622/1
type: Range
persistent identity: range2184622
display id: range2184622
version: 1
direction: direction/forward
top level: BBa_K863001
start: 1
end: 3
orientation: inline

bpul
type: SequenceAnnotation
persistent identity: annotation2184621
display id: annotation2184621
version: 1
top level: BBa_K863001
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K863001/annotation2184621/range2184621/1

https://synbiohub.org/public/igem/BBa_K863001/annotation2184621/range2184621/1
type: Range
persistent identity: range2184621
display id: range2184621
version: 1
direction: direction/forward
top level: BBa_K863001
start: 1
end: 1533
orientation: inline

Bioplastic
type: ComponentDefinition
persistent identity: BBa K156012
display id: BBa_K156012
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: phaA (acetyl-CoA acetyltransferase)
creator: George McArthur IV
created: 2008-10-28T12:00:00Z
modified: 2015-05-08T01:10:54Z
discontinued: false
dominant: false
experience: experience/None
group u list: _246_
m user id: 0
owner id: 1475
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part codes for an enzyme that catalyzes the synthesis of acetoacetyl-coenzyme A from two molecules of acetyl-coenzyme A. The enzyme can also act as a thiolase, catalyzing the reverse reaction and generating two-carbon units from the four-carbon product of fatty acid oxidation.
mutable notes: This part was codon-optimized for expression in E. coli. The following restriction enzyme sites were also removed: EcoRI, NotI, XbaI, SpeI, PstI, NheI, AvrII, ApoI, MfeI, NsiI, SbfI.
mutable provenance: This coding region is native to ''Ralstonia eutropha'' H16. The original sequence was obtained from GenBank (VERSION NC_008313.1, GI:113866031).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K156012_sequence/1

BBa_K2010001
type: ComponentDefinition
persistent identity: BBa K2010001
display id: BBa_K2010001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: ompT + PETase (PET-degrading enzyme, origin I. sakaiensis)
creator: William Cho
created: 2016-06-16T11:00:00Z
modified: 2016-10-17T01:23:21Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2477_
m user id: 30331
owner id: 30331
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: MHETase is the MHET(Mono(2-hydroxyethyl) terephthalate)-degrading enzyme first identified in Ideonella sakaiensis. This sequence is the E. coli K12 optimized DNA sequence for MHETase.
mutable notes: This sequence was optimized for E. coli K12 using the Codon Optimization Tool from the IDT website.
mutable provenance: This part comes from Ideonella sakaiensis, identified in "A bacterium that degrades and assimilates poly(ethylene terephthalate)" (Yoshida et al. 2016).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Start Codon
sequence annotation: T7 Promoter
sequence annotation: PETase
sequence annotation: Stop Codon
sequence annotation: GGS Linker
sequence annotation: RBS 34
sequence annotation: ompT Tag
sequence annotation: His-Tag
sequence: https://synbiohub.org/public/igem/BBa_K2010001_sequence/1

Start Codon
type: SequenceAnnotation
persistent identity: annotation2483566
display id: annotation2483566
version: 1
top level: BBa_K2010001
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K2010001/annotation2483566/range2483566/1

https://synbiohub.org/public/igem/BBa_K2010001/annotation2483566/range2483566/1
type: Range
persistent identity: range2483566
display id: range2483566
version: 1
direction: direction/forward
top level: BBa_K2010001
start: 59
end: 61
orientation: inline

T7 Promoter
type: SequenceAnnotation
persistent identity: annotation2480252
display id: annotation2480252
version: 1
top level: BBa_K2010001
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K2010001/annotation2480252/range2480252/1

https://synbiohub.org/public/igem/BBa_K2010001/annotation2480252/range2480252/1
type: Range
persistent identity: range2480252
display id: range2480252
version: 1
direction: direction/forward
top level: BBa_K2010001
start: 1
end: 46
orientation: inline

PETase
type: SequenceAnnotation
persistent identity: annotation2483555
display id: annotation2483555
version: 1
top level: BBa_K2010001
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K2010001/annotation2483555/range2483555/1

https://synbiohub.org/public/igem/BBa_K2010001/annotation2483555/range2483555/1
type: Range
persistent identity: range2483555
display id: range2483555
version: 1
direction: direction/forward
top level: BBa_K2010001
start: 155
end: 1024
orientation: inline

Stop Codon
type: SequenceAnnotation
persistent identity: annotation2483558
display id: annotation2483558
version: 1
top level: BBa_K2010001
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K2010001/annotation2483558/range2483558/1

https://synbiohub.org/public/igem/BBa_K2010001/annotation2483558/range2483558/1
type: Range
persistent identity: range2483558
display id: range2483558
version: 1
direction: direction/forward
top level: BBa_K2010001
start: 1079
end: 1084
orientation: inline

GGS Linker
type: SequenceAnnotation
persistent identity: annotation2514862
display id: annotation2514862
version: 1
top level: BBa_K2010001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K2010001/annotation2514862/range2514862/1

https://synbiohub.org/public/igem/BBa_K2010001/annotation2514862/range2514862/1
type: Range
persistent identity: range2514862
display id: range2514862
version: 1
direction: direction/forward
top level: BBa_K2010001
start: 119
end: 154
orientation: inline

RBS 34
type: SequenceAnnotation
persistent identity: annotation2480255
display id: annotation2480255
version: 1
top level: BBa_K2010001
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K2010001/annotation2480255/range2480255/1

https://synbiohub.org/public/igem/BBa_K2010001/annotation2480255/range2480255/1
type: Range
persistent identity: range2480255
display id: range2480255
version: 1
direction: direction/forward
top level: BBa_K2010001
start: 47
end: 58
orientation: inline

GGS Linker
type: SequenceAnnotation
persistent identity: annotation2483556
display id: annotation2483556
version: 1
top level: BBa_K2010001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K2010001/annotation2483556/range2483556/1

https://synbiohub.org/public/igem/BBa_K2010001/annotation2483556/range2483556/1
type: Range
persistent identity: range2483556
display id: range2483556
version: 1
direction: direction/forward
top level: BBa_K2010001
start: 1025
end: 1060
orientation: inline

ompT Tag
type: SequenceAnnotation
persistent identity: annotation2480254
display id: annotation2480254
version: 1
top level: BBa_K2010001
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K2010001/annotation2480254/range2480254/1

https://synbiohub.org/public/igem/BBa_K2010001/annotation2480254/range2480254/1
type: Range
persistent identity: range2480254
display id: range2480254
version: 1
direction: direction/forward
top level: BBa_K2010001
start: 59
end: 118
orientation: inline

His-Tag
type: SequenceAnnotation
persistent identity: annotation2483557
display id: annotation2483557
version: 1
top level: BBa_K2010001
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K2010001/annotation2483557/range2483557/1

https://synbiohub.org/public/igem/BBa_K2010001/annotation2483557/range2483557/1
type: Range
persistent identity: range2483557
display id: range2483557
version: 1
direction: direction/forward
top level: BBa_K2010001
start: 1061
end: 1078
orientation: inline

BBa_K338022
type: ComponentDefinition
persistent identity: BBa K338022
display id: BBa_K338022
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Plastic Intermediate: phaA + RBS
creator: Lucas Hartsough
created: 2010-10-26T11:00:00Z
modified: 2015-05-08T01:12:07Z
discontinued: false
dominant: false
group u list: _454_
m user id: 0
owner id: 6013
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: A construction intermediate used to produce <partinfo>BBa_K338003</partinfo>. Contains the first of three genes required to synthesize polyhydroxybutyrate (PHB).
mutable notes: Designed to be ligated upstream of <partinfo>BBa_K338023</partinfo> and <partinfo>BBa_K338024</partinfo>.
mutable provenance: The Registry of Standard Biological Parts
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K156012
component: BBa_B0034
sequence annotation: BBa_B0034
sequence annotation: BBa_K156012
sequence: https://synbiohub.org/public/igem/BBa_K338022_sequence/1

BBa_K156012
type: Component
persistent identity: component2104851
display id: component2104851
version: 1
top level: BBa_K338022
access: public
definition: Bioplastic

BBa_B0034
type: Component
persistent identity: component2104853
display id: component2104853
version: 1
top level: BBa_K338022
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2104853
display id: annotation2104853
version: 1
top level: BBa_K338022
location: https://synbiohub.org/public/igem/BBa_K338022/annotation2104853/range2104853/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K338022/annotation2104853/range2104853/1
type: Range
persistent identity: range2104853
display id: range2104853
version: 1
direction: direction/reverse
top level: BBa_K338022
start: 1191
end: 1202
orientation: inline

BBa_K156012
type: SequenceAnnotation
persistent identity: annotation2104851
display id: annotation2104851
version: 1
top level: BBa_K338022
location: https://synbiohub.org/public/igem/BBa_K338022/annotation2104851/range2104851/1
component: BBa_K156012

https://synbiohub.org/public/igem/BBa_K338022/annotation2104851/range2104851/1
type: Range
persistent identity: range2104851
display id: range2104851
version: 1
direction: direction/reverse
top level: BBa_K338022
start: 1
end: 1182
orientation: inline

BBa_K1165002
type: ComponentDefinition
persistent identity: BBa K1165002
display id: BBa_K1165002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PenI-cleaving Ribozyme 1 of XOR Gate 1
creator: iGEM Concordia 2013
created: 2013-09-26T11:00:00Z
modified: 2015-05-08T01:09:33Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1477_
m user id: 0
owner id: 18237
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is the DNA sequence of a Y-type hammerhead ribozyme that targets and cleaves the PenI repressor (Part BBa_C0074). This ribozyme has target-binding flanking arms adjacent to the Y-type hammerhead catalytic core and synthetic extended arms located towards the 5' and 3' ends. The catalytic core allows for the sequence-specific cleavage of sites within the coding sequence of PenI. Hammerhead ribozymes are able to cleave at any NUH within an RNA sequence, where H represent any nucleotide except guanine (Mir 2001), although with GUC as the hammerhead consensus cleavage site (Lieber, 1995). The binding of the flanking arms adjacent to the hammerhead catalytic core favours the recognition and cleavage of only one target site. Additional secondary structure within the flanking arms was detected to a small degree by using IDT OligoAnalyzer's Hairpin tool (Owczarzy 2008) as is depicted below. [image] The synthetic extended arms are designed to bind to the complementary extended arms of another similar PenI-cleaving ribozyme (Part _____), but do not contribute to any additional secondary structure as confirmed via the Hairpin tool in IDT's OligoAnalyzer (Owczarzy 2008), as both arms of a particular ribozyme only contain a purine and pyrimidine pair that do not bind to each other (in this case, A and G). The two-nucleotide extended arms are also staggered in number to avoid slippage in binding and bind to its complement pair at significantly higher melting temperatures than the adjacent flanking arms. This feature allows for the sequestering of the two ribozymes when both are present and significantly decreasing any ribozyme activity. Regions of complementarity between the two target-binding regions of both ribozymes are also present to ensure that the functional catalytic cores are not able to cleave their mRNA targets, even when bound. The characteristics of this expressed pair of ribozymes were considered to create a functional RNA-based XOR logic gate. References Mir, A…
mutable notes: Although this particular part is likely to only cleave the PenI repressor mRNA due to its sequence specificity, the simple in silico design of this ribozyme allows for the cleavage of virtually any RNA sequence containing a cleave site (NUH). This particular sequence was obtained by first scanning our target PenI mRNA for the optimal cut site GUC. The 10 nucleotides surrounding this cut site were obtained and the complement of this area was generated for the ribozyme flanking arms. The length of this region was decided by ideal melting temperature (51.4 degrees Celsius). Additional synthetic extended arms were subsequently added to the 5' and 3' ends of the sequence for binding to the complement ribozyme in an XOR gate. This region of non-pairing purines and pyrimidines was staggered in base repeats to avoid slippage of binding and was defined in length by significantly higher melting temperatures (approximately 68 degrees Celsius). Finally, the catalytic core of the Y-type hammerhead ribozyme was introduced at the appropriate region. Throughout this entire process, secondary was continually checked and minimized using IDT OligoAnalyzer's Hairpin tool (Owczarzy 2008). The complete ribozyme sequences was also BLASTed again the E.coli genome, which was our chassis and yielded insignificant results. Other factors such as differential length or target binding regions due to varying melting temperature and GC content were also considered during the in silico design of this ribozyme and its pair in the XOR logic gate. References Owczarzy, R. et al. IDT SciTools: a suite for analysis and design of nucleic acid oligomers. Nucleic Acids Res 36, W163???W169 (2008).
mutable provenance: The target-binding flanking arms of the ribozyme were obtained from the complement of the PenI Repressor sequence.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000834
role: partType/RNA
sequence annotation: Target-Binding Arms
sequence annotation: Catalytic Core
sequence annotation: TAA Bump
sequence annotation: Synthetic Extended Arms
sequence: https://synbiohub.org/public/igem/BBa_K1165002_sequence/1

Target-Binding Arms
type: SequenceAnnotation
persistent identity: annotation2364288
display id: annotation2364288
version: 1
top level: BBa_K1165002
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1165002/annotation2364288/range2364288/1

https://synbiohub.org/public/igem/BBa_K1165002/annotation2364288/range2364288/1
type: Range
persistent identity: range2364288
display id: range2364288
version: 1
direction: direction/forward
top level: BBa_K1165002
start: 70
end: 81
orientation: inline

Catalytic Core
type: SequenceAnnotation
persistent identity: annotation2364287
display id: annotation2364287
version: 1
top level: BBa_K1165002
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1165002/annotation2364287/range2364287/1

https://synbiohub.org/public/igem/BBa_K1165002/annotation2364287/range2364287/1
type: Range
persistent identity: range2364287
display id: range2364287
version: 1
direction: direction/forward
top level: BBa_K1165002
start: 44
end: 69
orientation: inline

TAA Bump
type: SequenceAnnotation
persistent identity: annotation2364286
display id: annotation2364286
version: 1
top level: BBa_K1165002
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1165002/annotation2364286/range2364286/1

https://synbiohub.org/public/igem/BBa_K1165002/annotation2364286/range2364286/1
type: Range
persistent identity: range2364286
display id: range2364286
version: 1
direction: direction/forward
top level: BBa_K1165002
start: 36
end: 38
orientation: inline

Target-Binding Arms
type: SequenceAnnotation
persistent identity: annotation2364285
display id: annotation2364285
version: 1
top level: BBa_K1165002
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1165002/annotation2364285/range2364285/1

https://synbiohub.org/public/igem/BBa_K1165002/annotation2364285/range2364285/1
type: Range
persistent identity: range2364285
display id: range2364285
version: 1
direction: direction/forward
top level: BBa_K1165002
start: 39
end: 43
orientation: inline

Synthetic Extended Arms
type: SequenceAnnotation
persistent identity: annotation2364283
display id: annotation2364283
version: 1
top level: BBa_K1165002
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1165002/annotation2364283/range2364283/1

https://synbiohub.org/public/igem/BBa_K1165002/annotation2364283/range2364283/1
type: Range
persistent identity: range2364283
display id: range2364283
version: 1
direction: direction/forward
top level: BBa_K1165002
start: 82
end: 111
orientation: inline

Synthetic Extended Arms
type: SequenceAnnotation
persistent identity: annotation2364282
display id: annotation2364282
version: 1
top level: BBa_K1165002
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1165002/annotation2364282/range2364282/1

https://synbiohub.org/public/igem/BBa_K1165002/annotation2364282/range2364282/1
type: Range
persistent identity: range2364282
display id: range2364282
version: 1
direction: direction/forward
top level: BBa_K1165002
start: 1
end: 30
orientation: inline

Target-Binding Arms
type: SequenceAnnotation
persistent identity: annotation2364284
display id: annotation2364284
version: 1
top level: BBa_K1165002
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1165002/annotation2364284/range2364284/1

https://synbiohub.org/public/igem/BBa_K1165002/annotation2364284/range2364284/1
type: Range
persistent identity: range2364284
display id: range2364284
version: 1
direction: direction/forward
top level: BBa_K1165002
start: 31
end: 35
orientation: inline

BBa_K863020
type: ComponentDefinition
persistent identity: BBa K863020
display id: BBa_K863020
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: bhal laccase from Bacillus halodurans with T7 promoter, RBS and His-tag
creator: Isabel Huber
created: 2012-09-18T11:00:00Z
modified: 2015-05-08T01:13:36Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1123_
m user id: 0
owner id: 12844
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: bhal laccase from Bacillus halodurans with T7 and HIS
mutable notes: test
mutable provenance: Bacillus halodurans C-125
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: 6 x HIS
sequence annotation: T7
sequence annotation: RBS
sequence annotation: TAA
sequence annotation: bhal
sequence annotation: ATG
sequence: https://synbiohub.org/public/igem/BBa_K863020_sequence/1

6 x HIS
type: SequenceAnnotation
persistent identity: annotation2202255
display id: annotation2202255
version: 1
top level: BBa_K863020
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K863020/annotation2202255/range2202255/1

https://synbiohub.org/public/igem/BBa_K863020/annotation2202255/range2202255/1
type: Range
persistent identity: range2202255
display id: range2202255
version: 1
direction: direction/forward
top level: BBa_K863020
start: 1534
end: 1551
orientation: inline

T7
type: SequenceAnnotation
persistent identity: annotation2202251
display id: annotation2202251
version: 1
top level: BBa_K863020
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K863020/annotation2202251/range2202251/1

https://synbiohub.org/public/igem/BBa_K863020/annotation2202251/range2202251/1
type: Range
persistent identity: range2202251
display id: range2202251
version: 1
direction: direction/forward
top level: BBa_K863020
start: 1
end: 23
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2202252
display id: annotation2202252
version: 1
top level: BBa_K863020
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K863020/annotation2202252/range2202252/1

https://synbiohub.org/public/igem/BBa_K863020/annotation2202252/range2202252/1
type: Range
persistent identity: range2202252
display id: range2202252
version: 1
direction: direction/forward
top level: BBa_K863020
start: 25
end: 30
orientation: inline

TAA
type: SequenceAnnotation
persistent identity: annotation2202256
display id: annotation2202256
version: 1
top level: BBa_K863020
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K863020/annotation2202256/range2202256/1

https://synbiohub.org/public/igem/BBa_K863020/annotation2202256/range2202256/1
type: Range
persistent identity: range2202256
display id: range2202256
version: 1
direction: direction/forward
top level: BBa_K863020
start: 1552
end: 1554
orientation: inline

TAA
type: SequenceAnnotation
persistent identity: annotation2202257
display id: annotation2202257
version: 1
top level: BBa_K863020
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K863020/annotation2202257/range2202257/1

https://synbiohub.org/public/igem/BBa_K863020/annotation2202257/range2202257/1
type: Range
persistent identity: range2202257
display id: range2202257
version: 1
direction: direction/forward
top level: BBa_K863020
start: 1555
end: 1557
orientation: inline

bhal
type: SequenceAnnotation
persistent identity: annotation2202254
display id: annotation2202254
version: 1
top level: BBa_K863020
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K863020/annotation2202254/range2202254/1

https://synbiohub.org/public/igem/BBa_K863020/annotation2202254/range2202254/1
type: Range
persistent identity: range2202254
display id: range2202254
version: 1
direction: direction/forward
top level: BBa_K863020
start: 34
end: 1533
orientation: inline

ATG
type: SequenceAnnotation
persistent identity: annotation2202253
display id: annotation2202253
version: 1
top level: BBa_K863020
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K863020/annotation2202253/range2202253/1

https://synbiohub.org/public/igem/BBa_K863020/annotation2202253/range2202253/1
type: Range
persistent identity: range2202253
display id: range2202253
version: 1
direction: direction/forward
top level: BBa_K863020
start: 34
end: 36
orientation: inline

BBa_K1031620
type: ComponentDefinition
persistent identity: BBa K1031620
display id: BBa_K1031620
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: NahF-Terminator
creator: He Shuaixin
created: 2013-09-08T11:00:00Z
modified: 2015-05-08T01:08:47Z
discontinued: false
dominant: false
group u list: _1338_
m user id: 0
owner id: 15843
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: NahF-TT
mutable notes: molecular cloning
mutable provenance: synthesis Pseudomonas
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: NahF
sequence annotation: B0015
sequence: https://synbiohub.org/public/igem/BBa_K1031620_sequence/1

NahF
type: SequenceAnnotation
persistent identity: annotation2360134
display id: annotation2360134
version: 1
top level: BBa_K1031620
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1031620/annotation2360134/range2360134/1

https://synbiohub.org/public/igem/BBa_K1031620/annotation2360134/range2360134/1
type: Range
persistent identity: range2360134
display id: range2360134
version: 1
direction: direction/forward
top level: BBa_K1031620
start: 1
end: 1452
orientation: inline

B0015
type: SequenceAnnotation
persistent identity: annotation2360136
display id: annotation2360136
version: 1
top level: BBa_K1031620
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1031620/annotation2360136/range2360136/1

https://synbiohub.org/public/igem/BBa_K1031620/annotation2360136/range2360136/1
type: Range
persistent identity: range2360136
display id: range2360136
version: 1
direction: direction/forward
top level: BBa_K1031620
start: 1459
end: 1587
orientation: inline

BBa_K1031622
type: ComponentDefinition
persistent identity: BBa K1031622
display id: BBa_K1031622
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: J23106-NahF-Terminator
creator: He Shuaixin
created: 2013-09-08T11:00:00Z
modified: 2015-05-08T01:08:47Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1338_
m user id: 0
owner id: 15843
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: 106-NahF-TT
mutable notes: molecular cloning
mutable provenance: synthesis Pseudomonas
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: NahF
sequence annotation: J23106
sequence annotation: B0015
sequence: https://synbiohub.org/public/igem/BBa_K1031622_sequence/1

NahF
type: SequenceAnnotation
persistent identity: annotation2361879
display id: annotation2361879
version: 1
top level: BBa_K1031622
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1031622/annotation2361879/range2361879/1

https://synbiohub.org/public/igem/BBa_K1031622/annotation2361879/range2361879/1
type: Range
persistent identity: range2361879
display id: range2361879
version: 1
direction: direction/forward
top level: BBa_K1031622
start: 62
end: 1513
orientation: inline

J23106
type: SequenceAnnotation
persistent identity: annotation2361878
display id: annotation2361878
version: 1
top level: BBa_K1031622
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1031622/annotation2361878/range2361878/1

https://synbiohub.org/public/igem/BBa_K1031622/annotation2361878/range2361878/1
type: Range
persistent identity: range2361878
display id: range2361878
version: 1
direction: direction/forward
top level: BBa_K1031622
start: 1
end: 35
orientation: inline

B0015
type: SequenceAnnotation
persistent identity: annotation2361881
display id: annotation2361881
version: 1
top level: BBa_K1031622
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1031622/annotation2361881/range2361881/1

https://synbiohub.org/public/igem/BBa_K1031622/annotation2361881/range2361881/1
type: Range
persistent identity: range2361881
display id: range2361881
version: 1
direction: direction/forward
top level: BBa_K1031622
start: 1519
end: 1648
orientation: inline

BBa_K808025
type: ComponentDefinition
persistent identity: BBa K808025
display id: BBa_K808025
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: FsC: Cutinase PET cleaving enzyme
creator: Marie Burghard, Henrik Cordes, Jascha Diemer, Adrian Eilingsfeld, Sven Jager, Rene Sahm, Arne Wehling
created: 2012-09-01T11:00:00Z
modified: 2015-05-08T01:13:26Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1065_
m user id: 0
owner id: 11792
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: A cutinase is a cuticula degrading hydrolase from the fungus Fusarium solani pisi. It shows activity towards PET.
mutable notes: Surface Expression is very hard to do. FsC shows lipolytic activity and attacks the outer membrane of gramm negativ bacteria. This activity leads to a certain toxicity for expression hosts. In addition FsC needs to be expressed in the periplasm due to folding issues.
mutable provenance: Cloned from vector pEST100 from Becker et all 2005: (Becker, S., Theile, S., Heppeler, N., Michalczyk, A., Wentzel, A., Wilhelm, S., Jaeger, K.-E., et al. (2005). A generic system for the Escherichia coli cell-surface display of lipolytic enzymes. FEBS letters, 579(5), 1177???82. doi:10.1016/j.febslet.2004.12.087)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K808025_sequence/1

BBa_I716253
type: ComponentDefinition
persistent identity: BBa I716253
display id: BBa_I716253
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: HPI, katG
creator: Vaibhavi Umesh
created: 2007-06-05T11:00:00Z
modified: 2015-08-31T04:07:51Z
discontinued: false
dominant: false
experience: experience/None
group u list: _110_
m user id: 0
owner id: 1696
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: A basic part required for the chaperone/toxicity device
mutable notes: {{BerkiGEM2007-Biobrick2.0Intro}} Construction of a Biobrick 2.0 basic part in plasmid pBca9145-Bca1144 (Clone # 5) for the HPI, katG open reading frame from Salmonella typhimurium LT2 PCR VU03F.katG /VU06R.katG on HPI, katG (1316 bp, gp = A) PCR VU05F.katG/VU04R.katG on HPI, katG (924 bp, gp = B) --------------------------------------------------- PCR VU03F/VU04R on A+B (2212 bp, BglII/XhoI) Digest pBca9145-Bca1144 (Clone # 5) (BglII/XhoI, 2063+910, L) Product is pBca9145-VU2 --------------------------------------------------- VU03F.katG (External oligo) ccaaaAGATCTatgagcacgaccgacgataccc VU04R.katG (External oligo) gctagCTCGAGttaGGATCCttattgcagatcgaaacggtcc VU05F.katG (Internal oligo) gccgaaagaagaCctgatctggcag VU06R.katG (Internal oligo) ctgccagatcagGtcttctttcggc
mutable provenance: Salmonella typhimurium LT2
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_I716253_sequence/1

BBa_K078007
type: ComponentDefinition
persistent identity: BBa K078007
display id: BBa_K078007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: 2,3-dihydroxy-2,3-dihydrophenylpropionate dehydrogenase, the second step enzyme in PCBs degradation
creator: Sally.Wang (Jingyi Wang)
created: 2008-10-19T11:00:00Z
modified: 2015-05-08T01:08:33Z
discontinued: false
dominant: false
group u list: _184_
m user id: 0
owner id: 2670
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: bphB codes 2,3-dihydroxy-2,3-dihydrophenylpropionate dehydrogenase, the second step enzyme in PCBs degradation pathway. Acoording to the standard, we add EcoR1,Not1 and Xba1 upstream; and spe1 Not1, Pst1 and stop condon downstream. The initial dioxygenase of the dioxin degradation pathway is a three-component enzyme with an atypical electron supply system.dxnA is one part of this muticomponent complex which acts as ring hydroxylase.dxnA harbors two subunits,dxnA1 and dxnA2. This is a standard parts. Acoording to the standard, we add EcoR1,Not1 and Xba1 upstream; and spe1, Not1, Pst1 and stop condon downstream. Anyone of the five sites could be used. The vector is the standard vector pSB1AC3 provided by biobrick08. We recommend double digestion of EcoRI+PstI.Digest in NEBuffer EcoRI + BSA at 37??C for about 3h.
mutable notes: We use it to construct the degradation pathway of PCBs. This is a standard parts. Acoording to the standard, we add EcoR1,Not1 and Xba1 upstream; and spe1, Not1, Pst1 and stop condon downstream. Anyone of the five sites could be used. The vector is the standard vector pSB1AC3 provided by biobrick08. We recommend double digestion of EcoRI+PstI.Digest in NEBuffer EcoRI + BSA at 37??C for about 3h.
mutable provenance: From the plasmids pAIA73 provided by Professor Bernd Holfer from Department of Microbiology, Gesellschaftfiir Biotechnologische Forschung, D-381 24 Braunschweig, Germany. Type: genomic DNA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: bphB
sequence: https://synbiohub.org/public/igem/BBa_K078007_sequence/1

bphB
type: SequenceAnnotation
persistent identity: annotation1983861
display id: annotation1983861
version: 1
top level: BBa_K078007
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K078007/annotation1983861/range1983861/1

https://synbiohub.org/public/igem/BBa_K078007/annotation1983861/range1983861/1
type: Range
persistent identity: range1983861
display id: range1983861
version: 1
direction: direction/forward
top level: BBa_K078007
start: 1
end: 830
orientation: inline

BBa_K863015
type: ComponentDefinition
persistent identity: BBa K863015
display id: BBa_K863015
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: xccl laccase from Xanthomonas campestris with T7 promoter, RBS and His-tag
creator: Isabel Huber
created: 2012-09-19T11:00:00Z
modified: 2015-05-08T01:13:36Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1123_
m user id: 0
owner id: 12844
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: xccl laccase from Xanthomonas campestris with pT7 and HIS tag
mutable notes: test
mutable provenance: Xanthomonas campestris pv. campestris B100
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: T7
sequence annotation: ATG
sequence annotation: 6x HIS
sequence annotation: TAA
sequence annotation: xccl
sequence: https://synbiohub.org/public/igem/BBa_K863015_sequence/1

T7
type: SequenceAnnotation
persistent identity: annotation2198670
display id: annotation2198670
version: 1
top level: BBa_K863015
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K863015/annotation2198670/range2198670/1

https://synbiohub.org/public/igem/BBa_K863015/annotation2198670/range2198670/1
type: Range
persistent identity: range2198670
display id: range2198670
version: 1
direction: direction/forward
top level: BBa_K863015
start: 1
end: 23
orientation: inline

ATG
type: SequenceAnnotation
persistent identity: annotation2198671
display id: annotation2198671
version: 1
top level: BBa_K863015
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K863015/annotation2198671/range2198671/1

https://synbiohub.org/public/igem/BBa_K863015/annotation2198671/range2198671/1
type: Range
persistent identity: range2198671
display id: range2198671
version: 1
direction: direction/forward
top level: BBa_K863015
start: 34
end: 36
orientation: inline

6x HIS
type: SequenceAnnotation
persistent identity: annotation2198699
display id: annotation2198699
version: 1
top level: BBa_K863015
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K863015/annotation2198699/range2198699/1

https://synbiohub.org/public/igem/BBa_K863015/annotation2198699/range2198699/1
type: Range
persistent identity: range2198699
display id: range2198699
version: 1
direction: direction/forward
top level: BBa_K863015
start: 1838
end: 1857
orientation: inline

TAA
type: SequenceAnnotation
persistent identity: annotation2198674
display id: annotation2198674
version: 1
top level: BBa_K863015
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K863015/annotation2198674/range2198674/1

https://synbiohub.org/public/igem/BBa_K863015/annotation2198674/range2198674/1
type: Range
persistent identity: range2198674
display id: range2198674
version: 1
direction: direction/forward
top level: BBa_K863015
start: 1861
end: 1863
orientation: inline

xccl
type: SequenceAnnotation
persistent identity: annotation2198673
display id: annotation2198673
version: 1
top level: BBa_K863015
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K863015/annotation2198673/range2198673/1

https://synbiohub.org/public/igem/BBa_K863015/annotation2198673/range2198673/1
type: Range
persistent identity: range2198673
display id: range2198673
version: 1
direction: direction/forward
top level: BBa_K863015
start: 34
end: 1837
orientation: inline

TAA
type: SequenceAnnotation
persistent identity: annotation2198697
display id: annotation2198697
version: 1
top level: BBa_K863015
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K863015/annotation2198697/range2198697/1

https://synbiohub.org/public/igem/BBa_K863015/annotation2198697/range2198697/1
type: Range
persistent identity: range2198697
display id: range2198697
version: 1
direction: direction/forward
top level: BBa_K863015
start: 1858
end: 1860
orientation: inline

ladA
type: ComponentDefinition
persistent identity: BBa K398000
display id: BBa_K398000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Long-Chain Alkane Monooxygenase
creator: Mathias Voges
created: 2010-06-11T11:00:00Z
modified: 2015-05-08T01:12:22Z
discontinued: false
dominant: false
experience: experience/None
group u list: _506_
m user id: 0
owner id: 6320
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Long-Chain Alkane Monooxygenase of Geobacillus thermodinitrificans.
mutable notes: None
mutable provenance: Genbank
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K398000_sequence/1

TetX
type: ComponentDefinition
persistent identity: BBa K2150101
display id: BBa_K2150101
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Tetracycline resistance protein from Bacteroides fragilis
creator: Zou Zhuoning
created: 2016-10-11T11:00:00Z
modified: 2016-10-16T11:38:42Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2620_
m user id: 29734
owner id: 29745
owning group id: 9
status: status/Planning
bookmark: true
mutable description: Tet X monooxygenase: Characterization: In vivo qualitative experiements：
mutable notes: protein sequence--gene sequence(coodon preferance) 3 synonynous mutaions
mutable provenance: Tetracycline resistance protein from transposon Tn4351/Tn4400 found in Bacteroides fragilis
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: TAA
sequence annotation: tetX
sequence: https://synbiohub.org/public/igem/BBa_K2150101_sequence/1

TAA
type: SequenceAnnotation
persistent identity: annotation2496159
display id: annotation2496159
version: 1
top level: TetX
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K2150101/annotation2496159/range2496159/1

https://synbiohub.org/public/igem/BBa_K2150101/annotation2496159/range2496159/1
type: Range
persistent identity: range2496159
display id: range2496159
version: 1
direction: direction/forward
top level: TetX
start: 1171
end: 1173
orientation: inline

tetX
type: SequenceAnnotation
persistent identity: annotation2496011
display id: annotation2496011
version: 1
top level: TetX
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K2150101/annotation2496011/range2496011/1

https://synbiohub.org/public/igem/BBa_K2150101/annotation2496011/range2496011/1
type: Range
persistent identity: range2496011
display id: range2496011
version: 1
direction: direction/forward
top level: TetX
start: 1
end: 1170
orientation: inline

BBa_K137014
type: ComponentDefinition
persistent identity: BBa K137014
display id: BBa_K137014
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: katE +LAA
creator: Doug Tischer
created: 2008-06-19T11:00:00Z
modified: 2015-05-08T01:10:08Z
discontinued: false
dominant: false
experience: experience/None
group u list: _187_
m user id: 0
owner id: 3049
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: katE (catalase) from E. coli. Site directed mutagensis to remove an EcoRI site, and an added C-term ssrA (LAA) tag for fast cell clearance.
mutable notes: Removal of an EcoRI site by site directed mutagenesis and addition of an ssrA tag with PCR primers
mutable provenance: E. coli genomic PCR and addition of ssrA tag with primers during PCR
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: LAA
sequence annotation: C->T
sequence annotation: katE
sequence: https://synbiohub.org/public/igem/BBa_K137014_sequence/1

LAA
type: SequenceAnnotation
persistent identity: annotation1969502
display id: annotation1969502
version: 1
top level: BBa_K137014
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K137014/annotation1969502/range1969502/1

https://synbiohub.org/public/igem/BBa_K137014/annotation1969502/range1969502/1
type: Range
persistent identity: range1969502
display id: range1969502
version: 1
direction: direction/forward
top level: BBa_K137014
start: 2260
end: 2292
orientation: inline

C->T
type: SequenceAnnotation
persistent identity: annotation1969500
display id: annotation1969500
version: 1
top level: BBa_K137014
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K137014/annotation1969500/range1969500/1

https://synbiohub.org/public/igem/BBa_K137014/annotation1969500/range1969500/1
type: Range
persistent identity: range1969500
display id: range1969500
version: 1
direction: direction/forward
top level: BBa_K137014
start: 1041
end: 1041
orientation: inline

katE
type: SequenceAnnotation
persistent identity: annotation1969501
display id: annotation1969501
version: 1
top level: BBa_K137014
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K137014/annotation1969501/range1969501/1

https://synbiohub.org/public/igem/BBa_K137014/annotation1969501/range1969501/1
type: Range
persistent identity: range1969501
display id: range1969501
version: 1
direction: direction/forward
top level: BBa_K137014
start: 1
end: 2298
orientation: inline

BBa_K1031931
type: ComponentDefinition
persistent identity: BBa K1031931
display id: BBa_K1031931
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: J23114-XylC-Terminator
creator: He Shuaixin
created: 2013-10-13T11:00:00Z
modified: 2015-05-08T01:08:48Z
discontinued: false
dominant: false
group u list: _1338_
m user id: 0
owner id: 15843
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: 114-XylC-TT
mutable notes: PCR & molecular cloning
mutable provenance: Pseudomonas.putida pWW0
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: XylC
sequence annotation: B0015
sequence annotation: J23114
sequence: https://synbiohub.org/public/igem/BBa_K1031931_sequence/1

XylC
type: SequenceAnnotation
persistent identity: annotation2369604
display id: annotation2369604
version: 1
top level: BBa_K1031931
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1031931/annotation2369604/range2369604/1

https://synbiohub.org/public/igem/BBa_K1031931/annotation2369604/range2369604/1
type: Range
persistent identity: range2369604
display id: range2369604
version: 1
direction: direction/forward
top level: BBa_K1031931
start: 54
end: 1517
orientation: inline

B0015
type: SequenceAnnotation
persistent identity: annotation2369605
display id: annotation2369605
version: 1
top level: BBa_K1031931
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1031931/annotation2369605/range2369605/1

https://synbiohub.org/public/igem/BBa_K1031931/annotation2369605/range2369605/1
type: Range
persistent identity: range2369605
display id: range2369605
version: 1
direction: direction/forward
top level: BBa_K1031931
start: 1518
end: 1646
orientation: inline

J23114
type: SequenceAnnotation
persistent identity: annotation2369603
display id: annotation2369603
version: 1
top level: BBa_K1031931
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1031931/annotation2369603/range2369603/1

https://synbiohub.org/public/igem/BBa_K1031931/annotation2369603/range2369603/1
type: Range
persistent identity: range2369603
display id: range2369603
version: 1
direction: direction/forward
top level: BBa_K1031931
start: 1
end: 35
orientation: inline

BBa_K808026
type: ComponentDefinition
persistent identity: BBa K808026
display id: BBa_K808026
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pNB-Est 13: Esterase PET cleaving enzyme
creator: Marie Burghard, Henrik Cordes, Jascha Diemer, Adrian Eilingsfeld, Sven Jager, Philipp Rottmann, Rene Sahm, Arne Wehling
created: 2012-09-01T11:00:00Z
modified: 2015-05-08T01:13:26Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1065_
m user id: 0
owner id: 11792
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: The pNB-ESterase 13 is a mutant derived from pNB-Esterase from Bacillus licheniformis. It showed the highest affinity and activity towards PET.
mutable notes: -
mutable provenance: Our team obtained it from Henkel GmbH on an pET26b(+) vector: Fakult, M., & Enzymtechnologie, M. (2006). Isolierung, Identifizierung und biochemische Charakterisierung Dialkylphthalat spaltender Esterasen.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K808026_sequence/1

BBa_K1355004
type: ComponentDefinition
persistent identity: BBa K1355004
display id: BBa_K1355004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Mercury ions bioremediating device
creator: Luna Barroco de Lacerda, Maria Clara Tavares Astolfi
created: 2014-10-05T11:00:00Z
modified: 2015-05-08T01:10:02Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1731_
m user id: 0
owner id: 15499
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: The biorremediator device is composed by our ???essential biobrick??? (BBa_K1355001) + a MerA gene (BBa_K1355000). In the presence of mercury, The MerR protein will bind to it and MerT, MerP and MerA protein will be expressed and bioremediation will start! The MerP and MerT proteins are responsible for the transport of mercury from the periplasm to the cytoplasm, leading him to the mercury ion reductase (MerA)! The beautiful MerA be responsible for the reduction from Hg2+ in Hg0, which is volatile at room temperature and able to passively leave out the bacteria.
mutable notes: For this construct, we followed these steps: Extraction of plasmid DNA and DNA quantification of bacteria transformed with the Essential Biobrick (BBa_K1355001) and the bacteria transformed with the MerA Biobrick (BBa_E0840); Verifying the electrophoretic profile of the extracted plasmid DNA; Restriction enzyme digestion for Essential Biobrick with SpeI and PstI and for merA Biobrick with PstI and XbaI; Checking the electrophoretic profile of digested samples; Fragment of interest purification from the gel; Ligation the gel purification of merA gene and digested Essential Biobrick; Transformation of ligation in DH5-alpha; Plasmid DNA extraction from bacteria transformed; Check the electrophoretic profile to see results of samples linked (no fragments); Digestion of Essential Biobrick + merA Biobrick with EcoRI and PstI, aiming to analyze the fragment size to be isolated; Checking the electrophoretic profile of the digested sample to obtain results showing that the isolated fragment is the junction of Essential Biobrick +merA Biobrick ;
mutable provenance: BBa_K1355001, BBa_K1355000
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_K1355000
component: BBa_K1355001
sequence annotation: BBa_K1355000
sequence annotation: BBa_K1355001
sequence: https://synbiohub.org/public/igem/BBa_K1355004_sequence/1

BBa_K1355000
type: Component
persistent identity: component2415200
display id: component2415200
version: 1
top level: BBa_K1355004
access: public
definition: https://synbiohub.org/public/igem/BBa_K1355000/1

BBa_K1355001
type: Component
persistent identity: component2415196
display id: component2415196
version: 1
top level: BBa_K1355004
access: public
definition: https://synbiohub.org/public/igem/BBa_K1355001/1

BBa_K1355000
type: SequenceAnnotation
persistent identity: annotation2415200
display id: annotation2415200
version: 1
top level: BBa_K1355004
location: https://synbiohub.org/public/igem/BBa_K1355004/annotation2415200/range2415200/1
component: BBa_K1355000

https://synbiohub.org/public/igem/BBa_K1355004/annotation2415200/range2415200/1
type: Range
persistent identity: range2415200
display id: range2415200
version: 1
direction: direction/reverse
top level: BBa_K1355004
start: 1198
end: 2975
orientation: inline

BBa_K1355001
type: SequenceAnnotation
persistent identity: annotation2415196
display id: annotation2415196
version: 1
top level: BBa_K1355004
location: https://synbiohub.org/public/igem/BBa_K1355004/annotation2415196/range2415196/1
component: BBa_K1355001

https://synbiohub.org/public/igem/BBa_K1355004/annotation2415196/range2415196/1
type: Range
persistent identity: range2415196
display id: range2415196
version: 1
direction: direction/reverse
top level: BBa_K1355004
start: 1
end: 1189
orientation: inline

BBa_K2010003
type: ComponentDefinition
persistent identity: BBa K2010003
display id: BBa_K2010003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: YebF + PETase (PET-degrading enzyme, origin I. sakaiensis)
creator: William Cho
created: 2016-06-20T11:00:00Z
modified: 2016-10-08T01:03:50Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2477_
m user id: 30331
owner id: 30331
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This GGS linker is a linker sequence that neutrally connects two protein domains together. The linker is composed of repetitions of glycine-glycine-serine, which are known to be neutral and not interfere with the rest of the protein. This is an alternate version of BBa_K2010002, but serves the same function.
mutable notes: The DNA sequence has been optimized for E. coli K12 and modified to facilitate gBlock synthesis, by avoiding repeated sequences.
mutable provenance: This sequence is publicly known and was privately modified.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: T7 Promoter
sequence annotation: GGS Linker
sequence annotation: Stop Codon
sequence annotation: PETase
sequence annotation: RBS 34
sequence annotation: His-Tag
sequence annotation: Start Codon
sequence annotation: YebF
sequence: https://synbiohub.org/public/igem/BBa_K2010003_sequence/1

T7 Promoter
type: SequenceAnnotation
persistent identity: annotation2480262
display id: annotation2480262
version: 1
top level: BBa_K2010003
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K2010003/annotation2480262/range2480262/1

https://synbiohub.org/public/igem/BBa_K2010003/annotation2480262/range2480262/1
type: Range
persistent identity: range2480262
display id: range2480262
version: 1
direction: direction/forward
top level: BBa_K2010003
start: 1
end: 46
orientation: inline

GGS Linker
type: SequenceAnnotation
persistent identity: annotation2483572
display id: annotation2483572
version: 1
top level: BBa_K2010003
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K2010003/annotation2483572/range2483572/1

https://synbiohub.org/public/igem/BBa_K2010003/annotation2483572/range2483572/1
type: Range
persistent identity: range2483572
display id: range2483572
version: 1
direction: direction/forward
top level: BBa_K2010003
start: 1319
end: 1354
orientation: inline

Stop Codon
type: SequenceAnnotation
persistent identity: annotation2483574
display id: annotation2483574
version: 1
top level: BBa_K2010003
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K2010003/annotation2483574/range2483574/1

https://synbiohub.org/public/igem/BBa_K2010003/annotation2483574/range2483574/1
type: Range
persistent identity: range2483574
display id: range2483574
version: 1
direction: direction/forward
top level: BBa_K2010003
start: 1373
end: 1378
orientation: inline

PETase
type: SequenceAnnotation
persistent identity: annotation2483570
display id: annotation2483570
version: 1
top level: BBa_K2010003
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K2010003/annotation2483570/range2483570/1

https://synbiohub.org/public/igem/BBa_K2010003/annotation2483570/range2483570/1
type: Range
persistent identity: range2483570
display id: range2483570
version: 1
direction: direction/forward
top level: BBa_K2010003
start: 449
end: 1318
orientation: inline

RBS 34
type: SequenceAnnotation
persistent identity: annotation2483567
display id: annotation2483567
version: 1
top level: BBa_K2010003
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K2010003/annotation2483567/range2483567/1

https://synbiohub.org/public/igem/BBa_K2010003/annotation2483567/range2483567/1
type: Range
persistent identity: range2483567
display id: range2483567
version: 1
direction: direction/forward
top level: BBa_K2010003
start: 47
end: 58
orientation: inline

His-Tag
type: SequenceAnnotation
persistent identity: annotation2483571
display id: annotation2483571
version: 1
top level: BBa_K2010003
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K2010003/annotation2483571/range2483571/1

https://synbiohub.org/public/igem/BBa_K2010003/annotation2483571/range2483571/1
type: Range
persistent identity: range2483571
display id: range2483571
version: 1
direction: direction/forward
top level: BBa_K2010003
start: 1355
end: 1372
orientation: inline

Start Codon
type: SequenceAnnotation
persistent identity: annotation2483573
display id: annotation2483573
version: 1
top level: BBa_K2010003
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K2010003/annotation2483573/range2483573/1

https://synbiohub.org/public/igem/BBa_K2010003/annotation2483573/range2483573/1
type: Range
persistent identity: range2483573
display id: range2483573
version: 1
direction: direction/forward
top level: BBa_K2010003
start: 59
end: 61
orientation: inline

YebF
type: SequenceAnnotation
persistent identity: annotation2483568
display id: annotation2483568
version: 1
top level: BBa_K2010003
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K2010003/annotation2483568/range2483568/1

https://synbiohub.org/public/igem/BBa_K2010003/annotation2483568/range2483568/1
type: Range
persistent identity: range2483568
display id: range2483568
version: 1
direction: direction/forward
top level: BBa_K2010003
start: 59
end: 412
orientation: inline

GGS Linker
type: SequenceAnnotation
persistent identity: annotation2483569
display id: annotation2483569
version: 1
top level: BBa_K2010003
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K2010003/annotation2483569/range2483569/1

https://synbiohub.org/public/igem/BBa_K2010003/annotation2483569/range2483569/1
type: Range
persistent identity: range2483569
display id: range2483569
version: 1
direction: direction/forward
top level: BBa_K2010003
start: 413
end: 448
orientation: inline

BBa_K863006
type: ComponentDefinition
persistent identity: BBa K863006
display id: BBa_K863006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: ecol laccase from E. coli
creator: Isabel Huber
created: 2012-09-17T11:00:00Z
modified: 2015-05-08T01:13:36Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1123_
m user id: 0
owner id: 12844
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: E.coli laccase ORF
mutable notes: test
mutable provenance: E. coli BL21 (DE3)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: ATG
sequence annotation: TAA
sequence: https://synbiohub.org/public/igem/BBa_K863006_sequence/1

ATG
type: SequenceAnnotation
persistent identity: annotation2184435
display id: annotation2184435
version: 1
top level: BBa_K863006
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K863006/annotation2184435/range2184435/1

https://synbiohub.org/public/igem/BBa_K863006/annotation2184435/range2184435/1
type: Range
persistent identity: range2184435
display id: range2184435
version: 1
direction: direction/forward
top level: BBa_K863006
start: 1
end: 3
orientation: inline

TAA
type: SequenceAnnotation
persistent identity: annotation2184436
display id: annotation2184436
version: 1
top level: BBa_K863006
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K863006/annotation2184436/range2184436/1

https://synbiohub.org/public/igem/BBa_K863006/annotation2184436/range2184436/1
type: Range
persistent identity: range2184436
display id: range2184436
version: 1
direction: direction/forward
top level: BBa_K863006
start: 1549
end: 1551
orientation: inline

BBa_K2010002
type: ComponentDefinition
persistent identity: BBa K2010002
display id: BBa_K2010002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pelB + PETase (PET-degrading enzyme, origin I. sakaiensis)
creator: William Cho
created: 2016-06-20T11:00:00Z
modified: 2016-10-17T01:26:20Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2477_
m user id: 30331
owner id: 30331
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This GGS linker is a linker sequence that neutrally connects two protein domains together. The linker is composed of repetitions of glycine-glycine-serine, which are known to be neutral and not interfere with the rest of the protein.
mutable notes: The DNA sequence has been optimized for E. coli K12 and modified to facilitate gBlock synthesis, by avoiding repeated sequences.
mutable provenance: This sequence is publicly known and was privately modified.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Start Codon
sequence annotation: GGS Linker
sequence annotation: Stop Codon
sequence annotation: PETase
sequence annotation: His-Tag
sequence annotation: RBS 34
sequence annotation: T7 Promoter
sequence annotation: pelB Tag
sequence: https://synbiohub.org/public/igem/BBa_K2010002_sequence/1

Start Codon
type: SequenceAnnotation
persistent identity: annotation2483565
display id: annotation2483565
version: 1
top level: BBa_K2010002
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K2010002/annotation2483565/range2483565/1

https://synbiohub.org/public/igem/BBa_K2010002/annotation2483565/range2483565/1
type: Range
persistent identity: range2483565
display id: range2483565
version: 1
direction: direction/forward
top level: BBa_K2010002
start: 59
end: 61
orientation: inline

GGS Linker
type: SequenceAnnotation
persistent identity: annotation2514866
display id: annotation2514866
version: 1
top level: BBa_K2010002
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K2010002/annotation2514866/range2514866/1

https://synbiohub.org/public/igem/BBa_K2010002/annotation2514866/range2514866/1
type: Range
persistent identity: range2514866
display id: range2514866
version: 1
direction: direction/forward
top level: BBa_K2010002
start: 125
end: 160
orientation: inline

Stop Codon
type: SequenceAnnotation
persistent identity: annotation2483564
display id: annotation2483564
version: 1
top level: BBa_K2010002
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K2010002/annotation2483564/range2483564/1

https://synbiohub.org/public/igem/BBa_K2010002/annotation2483564/range2483564/1
type: Range
persistent identity: range2483564
display id: range2483564
version: 1
direction: direction/forward
top level: BBa_K2010002
start: 1085
end: 1090
orientation: inline

PETase
type: SequenceAnnotation
persistent identity: annotation2483561
display id: annotation2483561
version: 1
top level: BBa_K2010002
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K2010002/annotation2483561/range2483561/1

https://synbiohub.org/public/igem/BBa_K2010002/annotation2483561/range2483561/1
type: Range
persistent identity: range2483561
display id: range2483561
version: 1
direction: direction/forward
top level: BBa_K2010002
start: 161
end: 1030
orientation: inline

GGS Linker
type: SequenceAnnotation
persistent identity: annotation2483562
display id: annotation2483562
version: 1
top level: BBa_K2010002
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K2010002/annotation2483562/range2483562/1

https://synbiohub.org/public/igem/BBa_K2010002/annotation2483562/range2483562/1
type: Range
persistent identity: range2483562
display id: range2483562
version: 1
direction: direction/forward
top level: BBa_K2010002
start: 1031
end: 1066
orientation: inline

His-Tag
type: SequenceAnnotation
persistent identity: annotation2483563
display id: annotation2483563
version: 1
top level: BBa_K2010002
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K2010002/annotation2483563/range2483563/1

https://synbiohub.org/public/igem/BBa_K2010002/annotation2483563/range2483563/1
type: Range
persistent identity: range2483563
display id: range2483563
version: 1
direction: direction/forward
top level: BBa_K2010002
start: 1067
end: 1084
orientation: inline

RBS 34
type: SequenceAnnotation
persistent identity: annotation2483559
display id: annotation2483559
version: 1
top level: BBa_K2010002
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K2010002/annotation2483559/range2483559/1

https://synbiohub.org/public/igem/BBa_K2010002/annotation2483559/range2483559/1
type: Range
persistent identity: range2483559
display id: range2483559
version: 1
direction: direction/forward
top level: BBa_K2010002
start: 47
end: 58
orientation: inline

T7 Promoter
type: SequenceAnnotation
persistent identity: annotation2480261
display id: annotation2480261
version: 1
top level: BBa_K2010002
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K2010002/annotation2480261/range2480261/1

https://synbiohub.org/public/igem/BBa_K2010002/annotation2480261/range2480261/1
type: Range
persistent identity: range2480261
display id: range2480261
version: 1
direction: direction/forward
top level: BBa_K2010002
start: 1
end: 46
orientation: inline

pelB Tag
type: SequenceAnnotation
persistent identity: annotation2483560
display id: annotation2483560
version: 1
top level: BBa_K2010002
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K2010002/annotation2483560/range2483560/1

https://synbiohub.org/public/igem/BBa_K2010002/annotation2483560/range2483560/1
type: Range
persistent identity: range2483560
display id: range2483560
version: 1
direction: direction/forward
top level: BBa_K2010002
start: 59
end: 124
orientation: inline

BBa_K078008
type: ComponentDefinition
persistent identity: BBa K078008
display id: BBa_K078008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: bphC codes 2,3-Dihydroxybiphenyl-1,2-dioxygenase
creator: Sally.Wang (Jingyi Wang)
created: 2008-10-21T11:00:00Z
modified: 2015-05-08T01:08:33Z
discontinued: false
dominant: false
experience: experience/None
group u list: _184_
m user id: 0
owner id: 2670
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: bphC codes 2,3-Dihydroxybiphenyl-1,2-dioxygenase that is the third step enzyme in PCBs degradation pathway. This is a standard parts. Acoording to the standard, we add EcoR1,Not1 and Xba1 upstream; and spe1, Not1, Pst1 and stop condon downstream. Anyone of the five sites could be used. The vector is the standard vector, pSB1AC3, provided by biobrick08. We recommend double digestion of EcoRI+pstI.Digest in NEBBuffer EcoRI + BSA at 37??C for about 3h.
mutable notes: We use this part to construct the degradation pathway of PCBs.
mutable provenance: From the PCBs-degrading bacterium Burkholderia xenovorans LB400 chromosome 3. Type: genomic DNA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: bphC
sequence: https://synbiohub.org/public/igem/BBa_K078008_sequence/1

bphC
type: SequenceAnnotation
persistent identity: annotation1983851
display id: annotation1983851
version: 1
top level: BBa_K078008
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K078008/annotation1983851/range1983851/1

https://synbiohub.org/public/igem/BBa_K078008/annotation1983851/range1983851/1
type: Range
persistent identity: range1983851
display id: range1983851
version: 1
direction: direction/forward
top level: BBa_K078008
start: 1
end: 898
orientation: inline

Nuc-T7
type: ComponentDefinition
persistent identity: BBa K2144000
display id: BBa_K2144000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Coding sequence for Nuclease regulated by T7-promoter
creator: Oskar ??hman
created: 2016-09-21T11:00:00Z
modified: 2016-10-19T01:52:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2614_
m user id: 31341
owner id: 31517
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Usage: Nuclease is an enzyme with the capability of cleaving phosphodiester bonds between nucleotides in DNA. This enzyme can be used in applications concerning degradation of DNA. In our project the aim was to evaluate and use Nuclease degrading abilities to inhibit the biofilm formation produced by Staphyloccus Auerus. The encoding part of the BioBrick is derived from BBa_K729004, made by iGEM London 2012. The T7 promoter (BBa_k525998) has been inserted to control protein expession under IPTG induction. To regulate the protein expression under T7 promoter control, E.coli containing a T7 polymerase must be used, for instance BL21(DE3). Biology: Nuclease is an extracellular enzyme found in Staphyloccus Aureus. [3] S.Aureus encodes for two Nucleases, Nuc1 and Nuc2, [3] whereof the last-mentioned is a part of this BioBrick. Nuc2 is connected to the membrane with a N-terminal anchor. [3] There are several bacterial pathogens using Nuclease to combat the immune response in the host by for instance avoiding neutrophil extracellular traps. (NETs). [1] Furthermore, since eDNA is an important component of biofilm [2] Nuc is able to work as a regulator of biofilm formation. [2] Studies have suggested that the expression of Nuc contributes to the inability to produce biofilm [2] and during biofilm formation-conditions the expression of Nuc is repressed. [3]
mutable notes: The subparts was aligned by 3A Assembly.
mutable provenance: Nuclease from Staphylococcus Aureus.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_K729004
component: BBa_K525998
sequence annotation: BBa_K729004
sequence annotation: BBa_K525998
sequence: https://synbiohub.org/public/igem/BBa_K2144000_sequence/1

BBa_K729004
type: Component
persistent identity: component2484440
display id: component2484440
version: 1
top level: Nuc-T7
access: public
definition: https://synbiohub.org/public/igem/BBa_K729004/1

BBa_K525998
type: Component
persistent identity: component2484439
display id: component2484439
version: 1
top level: Nuc-T7
access: public
definition: https://synbiohub.org/public/igem/BBa_K525998/1

BBa_K729004
type: SequenceAnnotation
persistent identity: annotation2484440
display id: annotation2484440
version: 1
top level: Nuc-T7
location: https://synbiohub.org/public/igem/BBa_K2144000/annotation2484440/range2484440/1
component: BBa_K729004

https://synbiohub.org/public/igem/BBa_K2144000/annotation2484440/range2484440/1
type: Range
persistent identity: range2484440
display id: range2484440
version: 1
direction: direction/reverse
top level: Nuc-T7
start: 39
end: 599
orientation: inline

BBa_K525998
type: SequenceAnnotation
persistent identity: annotation2484439
display id: annotation2484439
version: 1
top level: Nuc-T7
location: https://synbiohub.org/public/igem/BBa_K2144000/annotation2484439/range2484439/1
component: BBa_K525998

https://synbiohub.org/public/igem/BBa_K2144000/annotation2484439/range2484439/1
type: Range
persistent identity: range2484439
display id: range2484439
version: 1
direction: direction/reverse
top level: Nuc-T7
start: 1
end: 32
orientation: inline

Mdaa
type: ComponentDefinition
persistent identity: BBa M36782
display id: BBa_M36782
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PoPS -> Melamine deaminase
creator: Ray Paul Laureano-Mercado & Mark Kirollos
created: 2013-06-09T11:00:00Z
modified: 2015-05-08T01:14:06Z
discontinued: false
dominant: false
experience: experience/None
group u list: _848_
m user id: 0
owner id: 17944
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This actuator's purpose is to code for the output of the enzyme melamine deaminase. This enzyme eliminates an amine group from melamine to produce ammonia and ammeline and is able to remove another amine group at a rate 10 times slower than the first reaction to bring the compound to ammelide. The enzyme does not react to a measurable extent with this last compound, so it metabolizes melamine by consecutive and predictable deaminations. Its design comprises three components, which include a bicistronic ribosome binding site, the MDA gene of interest which codes from melamine deaminase, and a transcription terminator. The part needs a PoPS signal as an input in order to produce the desired enzyme and was designed to work in E. coli.
mutable notes: The TriA gene???s codons were modified from the original sequence and optimized for E. coli to a Codon Adaptation Index (CAI) of .76 (Puigb??, P., Guzm??n, E., Romeu, A. & Garcia-Vallv??, S. OPTIMIZER: A web server for optimizing the codon usage of DNA sequences. Nucleic Acids Research. 2007;35:W126???31.). After that, some palindromic and mRNA destabilizing sequences were removed to increase the stability of our DNA during synthesis. The sequence shown maintains a CAI of .76 and the same amino acid sequence as the original TriA gene.
mutable provenance: The original gene coding for the protein of interest was isolated from a species of Pseudomonas (http://www.ncbi.nlm.nih.gov/nuccore/11890744). The sequences for the RBS and Terminator were sourced from BIOFAB (http://biofab.org/)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_M36761
component: BBa_M36236
component: BBa_B1002
sequence annotation: BBa_M36761
sequence annotation: BBa_B1002
sequence annotation: BBa_M36236
sequence: https://synbiohub.org/public/igem/BBa_M36782_sequence/1

BBa_M36761
type: Component
persistent identity: component2329370
display id: component2329370
version: 1
top level: Mdaa
access: public
definition: https://synbiohub.org/public/igem/BBa_M36761/1

BBa_M36236
type: Component
persistent identity: component2329369
display id: component2329369
version: 1
top level: Mdaa
access: public
definition: https://synbiohub.org/public/igem/BBa_M36236/1

BBa_B1002
type: Component
persistent identity: component2329375
display id: component2329375
version: 1
top level: Mdaa
access: public
definition: https://synbiohub.org/public/igem/BBa_B1002/1

BBa_M36761
type: SequenceAnnotation
persistent identity: annotation2329370
display id: annotation2329370
version: 1
top level: Mdaa
location: https://synbiohub.org/public/igem/BBa_M36782/annotation2329370/range2329370/1
component: BBa_M36761

https://synbiohub.org/public/igem/BBa_M36782/annotation2329370/range2329370/1
type: Range
persistent identity: range2329370
display id: range2329370
version: 1
direction: direction/reverse
top level: Mdaa
start: 86
end: 1510
orientation: inline

BBa_B1002
type: SequenceAnnotation
persistent identity: annotation2329375
display id: annotation2329375
version: 1
top level: Mdaa
location: https://synbiohub.org/public/igem/BBa_M36782/annotation2329375/range2329375/1
component: BBa_B1002

https://synbiohub.org/public/igem/BBa_M36782/annotation2329375/range2329375/1
type: Range
persistent identity: range2329375
display id: range2329375
version: 1
direction: direction/reverse
top level: Mdaa
start: 1511
end: 1544
orientation: inline

BBa_M36236
type: SequenceAnnotation
persistent identity: annotation2329369
display id: annotation2329369
version: 1
top level: Mdaa
location: https://synbiohub.org/public/igem/BBa_M36782/annotation2329369/range2329369/1
component: BBa_M36236

https://synbiohub.org/public/igem/BBa_M36782/annotation2329369/range2329369/1
type: Range
persistent identity: range2329369
display id: range2329369
version: 1
direction: direction/reverse
top level: Mdaa
start: 1
end: 85
orientation: inline

BBa_K2010005
type: ComponentDefinition
persistent identity: BBa K2010005
display id: BBa_K2010005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: ompT + sfGFP + PETase (PET-degrading enzyme, origin I. sakaiensis)
creator: William Cho
created: 2016-06-20T11:00:00Z
modified: 2016-10-08T01:21:43Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2477_
m user id: 30331
owner id: 30331
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This His-tag is a sequence that allows a conjunct protein to be purified. The tag is composed of repetitions of histidines. This is an alternate version of BBa_K2010004, but serves the same function.
mutable notes: The DNA sequence has been optimized for E. coli K12 and modified to facilitate gBlock synthesis, by avoiding repeated sequences.
mutable provenance: This sequence is publicly known and was privately modified.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Stop Codon
sequence annotation: PETase
sequence annotation: Linker
sequence annotation: T7 Promoter
sequence annotation: ompT Tag
sequence annotation: RBS 34
sequence annotation: His-Tag
sequence annotation: sfGFP
sequence: https://synbiohub.org/public/igem/BBa_K2010005_sequence/1

Stop Codon
type: SequenceAnnotation
persistent identity: annotation2483592
display id: annotation2483592
version: 1
top level: BBa_K2010005
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K2010005/annotation2483592/range2483592/1

https://synbiohub.org/public/igem/BBa_K2010005/annotation2483592/range2483592/1
type: Range
persistent identity: range2483592
display id: range2483592
version: 1
direction: direction/forward
top level: BBa_K2010005
start: 1784
end: 1789
orientation: inline

PETase
type: SequenceAnnotation
persistent identity: annotation2483588
display id: annotation2483588
version: 1
top level: BBa_K2010005
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K2010005/annotation2483588/range2483588/1

https://synbiohub.org/public/igem/BBa_K2010005/annotation2483588/range2483588/1
type: Range
persistent identity: range2483588
display id: range2483588
version: 1
direction: direction/forward
top level: BBa_K2010005
start: 860
end: 1729
orientation: inline

Linker
type: SequenceAnnotation
persistent identity: annotation2483589
display id: annotation2483589
version: 1
top level: BBa_K2010005
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K2010005/annotation2483589/range2483589/1

https://synbiohub.org/public/igem/BBa_K2010005/annotation2483589/range2483589/1
type: Range
persistent identity: range2483589
display id: range2483589
version: 1
direction: direction/forward
top level: BBa_K2010005
start: 1730
end: 1765
orientation: inline

T7 Promoter
type: SequenceAnnotation
persistent identity: annotation2480264
display id: annotation2480264
version: 1
top level: BBa_K2010005
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K2010005/annotation2480264/range2480264/1

https://synbiohub.org/public/igem/BBa_K2010005/annotation2480264/range2480264/1
type: Range
persistent identity: range2480264
display id: range2480264
version: 1
direction: direction/forward
top level: BBa_K2010005
start: 1
end: 46
orientation: inline

Linker
type: SequenceAnnotation
persistent identity: annotation2483587
display id: annotation2483587
version: 1
top level: BBa_K2010005
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K2010005/annotation2483587/range2483587/1

https://synbiohub.org/public/igem/BBa_K2010005/annotation2483587/range2483587/1
type: Range
persistent identity: range2483587
display id: range2483587
version: 1
direction: direction/forward
top level: BBa_K2010005
start: 845
end: 859
orientation: inline

ompT Tag
type: SequenceAnnotation
persistent identity: annotation2483584
display id: annotation2483584
version: 1
top level: BBa_K2010005
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K2010005/annotation2483584/range2483584/1

https://synbiohub.org/public/igem/BBa_K2010005/annotation2483584/range2483584/1
type: Range
persistent identity: range2483584
display id: range2483584
version: 1
direction: direction/forward
top level: BBa_K2010005
start: 65
end: 124
orientation: inline

RBS 34
type: SequenceAnnotation
persistent identity: annotation2483583
display id: annotation2483583
version: 1
top level: BBa_K2010005
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K2010005/annotation2483583/range2483583/1

https://synbiohub.org/public/igem/BBa_K2010005/annotation2483583/range2483583/1
type: Range
persistent identity: range2483583
display id: range2483583
version: 1
direction: direction/forward
top level: BBa_K2010005
start: 47
end: 58
orientation: inline

His-Tag
type: SequenceAnnotation
persistent identity: annotation2483591
display id: annotation2483591
version: 1
top level: BBa_K2010005
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K2010005/annotation2483591/range2483591/1

https://synbiohub.org/public/igem/BBa_K2010005/annotation2483591/range2483591/1
type: Range
persistent identity: range2483591
display id: range2483591
version: 1
direction: direction/forward
top level: BBa_K2010005
start: 1766
end: 1783
orientation: inline

Linker
type: SequenceAnnotation
persistent identity: annotation2483585
display id: annotation2483585
version: 1
top level: BBa_K2010005
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K2010005/annotation2483585/range2483585/1

https://synbiohub.org/public/igem/BBa_K2010005/annotation2483585/range2483585/1
type: Range
persistent identity: range2483585
display id: range2483585
version: 1
direction: direction/forward
top level: BBa_K2010005
start: 125
end: 130
orientation: inline

sfGFP
type: SequenceAnnotation
persistent identity: annotation2483586
display id: annotation2483586
version: 1
top level: BBa_K2010005
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K2010005/annotation2483586/range2483586/1

https://synbiohub.org/public/igem/BBa_K2010005/annotation2483586/range2483586/1
type: Range
persistent identity: range2483586
display id: range2483586
version: 1
direction: direction/forward
top level: BBa_K2010005
start: 131
end: 844
orientation: inline

BBa_K078006
type: ComponentDefinition
persistent identity: BBa K078006
display id: BBa_K078006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: One part of the initial dioxygenase in dioxin degradation pathway
creator: Sally.Wang (Jingyi Wang)
created: 2008-10-15T11:00:00Z
modified: 2015-05-08T01:08:33Z
discontinued: false
dominant: false
group u list: _184_
m user id: 0
owner id: 2670
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: The initial dioxygenase of the dioxin degradation pathway is a three-component enzyme with an atypical electron supply system. RedA2 is one part of this muticomponent complex which acts as reductase. This is a standard parts. Acoording to the standard, we add EcoR1,Not1 and Xba1 upstream; and spe1, Not1, Pst1 and stop condon downstream. Anyone of the five sites could be used except pst1. The vector is the standard vector provided by biobrick08. We recommend double digestion of EcoRI+SpeI.Digest in NEBuffer EcoRI + BSA at 37??C for about 3h.
mutable notes: We use this part to construct the degradation pathway of dioxin. The initial dioxygenase of the dioxin degradation pathway is a three-component enzyme with an atypical electron supply system. RedA2 is one part of this muticomponent complex which acts as reductase. This is a standard parts. Acoording to the standard, we add EcoR1,Not1 and Xba1 upstream; and spe1, Not1, Pst1 and stop condon downstream. Anyone of the five sites could be used except pst1. The vector is the standard vector provided by biobrick08. We recommend double digestion of EcoRI+SpeI.Digest in NEBuffer EcoRI + BSA at 37??C for about 3h.
mutable provenance: RedA2 is a reductase from the dibenzofuran- and dibenzo-p-dioxin-degrading bacterium Sphingomonas sp. strain RW1. Type: genomic DNA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: redA2
sequence: https://synbiohub.org/public/igem/BBa_K078006_sequence/1

redA2
type: SequenceAnnotation
persistent identity: annotation1983862
display id: annotation1983862
version: 1
top level: BBa_K078006
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K078006/annotation1983862/range1983862/1

https://synbiohub.org/public/igem/BBa_K078006/annotation1983862/range1983862/1
type: Range
persistent identity: range1983862
display id: range1983862
version: 1
direction: direction/forward
top level: BBa_K078006
start: 1
end: 1233
orientation: inline

BBa_K863021
type: ComponentDefinition
persistent identity: BBa K863021
display id: BBa_K863021
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: bhal laccase from Bacillus halodurans
creator: Isabel Huber
created: 2012-09-22T11:00:00Z
modified: 2015-05-08T01:13:36Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1123_
m user id: 0
owner id: 12844
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: bhal laccase from Bacillus halodurans
mutable notes: test
mutable provenance: Bacillus halodurans C-125
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: TAA
sequence annotation: ATG
sequence annotation: CDS
sequence: https://synbiohub.org/public/igem/BBa_K863021_sequence/1

TAA
type: SequenceAnnotation
persistent identity: annotation2213231
display id: annotation2213231
version: 1
top level: BBa_K863021
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K863021/annotation2213231/range2213231/1

https://synbiohub.org/public/igem/BBa_K863021/annotation2213231/range2213231/1
type: Range
persistent identity: range2213231
display id: range2213231
version: 1
direction: direction/forward
top level: BBa_K863021
start: 1501
end: 1503
orientation: inline

ATG
type: SequenceAnnotation
persistent identity: annotation2213230
display id: annotation2213230
version: 1
top level: BBa_K863021
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K863021/annotation2213230/range2213230/1

https://synbiohub.org/public/igem/BBa_K863021/annotation2213230/range2213230/1
type: Range
persistent identity: range2213230
display id: range2213230
version: 1
direction: direction/forward
top level: BBa_K863021
start: 1
end: 3
orientation: inline

CDS
type: SequenceAnnotation
persistent identity: annotation2213229
display id: annotation2213229
version: 1
top level: BBa_K863021
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K863021/annotation2213229/range2213229/1

https://synbiohub.org/public/igem/BBa_K863021/annotation2213229/range2213229/1
type: Range
persistent identity: range2213229
display id: range2213229
version: 1
direction: direction/forward
top level: BBa_K863021
start: 1
end: 1503
orientation: inline

alkJ
type: ComponentDefinition
persistent identity: BBa K826001
display id: BBa_K826001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: converts aliphatic medium-chain-length alcohols into aldehydes
creator: Kaige Zhang
created: 2012-09-26T11:00:00Z
modified: 2015-05-08T01:13:30Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1084_
m user id: 0
owner id: 12311
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: this gene is the next step of hydrocarbon degradation, specially for the medium-chain alcohols, wchih derived from P450(BBa_826000).
mutable notes: it can coupled with P450(BBa_826000) to work.
mutable provenance: Alcanivorax borkumensis SK2
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K826001_sequence/1

BBa_K2010007
type: ComponentDefinition
persistent identity: BBa K2010007
display id: BBa_K2010007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pelB + sfGFP + PETase (PET-degrading enzyme, origin I. sakaiensis)
creator: William Cho
created: 2016-09-15T11:00:00Z
modified: 2016-10-08T01:30:40Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2477_
m user id: 30331
owner id: 30331
owning group id: 9
status: status/Planning
bookmark: false
mutable description: PETase + pelB + sfGFP
mutable notes: PETase + pelB + sfGFP
mutable provenance: PETase + pelB + sfGFP
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Linker
sequence annotation: His-Tag
sequence annotation: pelB Tag
sequence annotation: Stop Codon
sequence annotation: PETase
sequence annotation: GGS Linker
sequence annotation: T7 Promoter
sequence annotation: sfGFP
sequence annotation: RBS 34
sequence: https://synbiohub.org/public/igem/BBa_K2010007_sequence/1

Linker
type: SequenceAnnotation
persistent identity: annotation2483610
display id: annotation2483610
version: 1
top level: BBa_K2010007
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K2010007/annotation2483610/range2483610/1

https://synbiohub.org/public/igem/BBa_K2010007/annotation2483610/range2483610/1
type: Range
persistent identity: range2483610
display id: range2483610
version: 1
direction: direction/forward
top level: BBa_K2010007
start: 851
end: 865
orientation: inline

Linker
type: SequenceAnnotation
persistent identity: annotation2483608
display id: annotation2483608
version: 1
top level: BBa_K2010007
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K2010007/annotation2483608/range2483608/1

https://synbiohub.org/public/igem/BBa_K2010007/annotation2483608/range2483608/1
type: Range
persistent identity: range2483608
display id: range2483608
version: 1
direction: direction/forward
top level: BBa_K2010007
start: 131
end: 136
orientation: inline

His-Tag
type: SequenceAnnotation
persistent identity: annotation2483613
display id: annotation2483613
version: 1
top level: BBa_K2010007
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K2010007/annotation2483613/range2483613/1

https://synbiohub.org/public/igem/BBa_K2010007/annotation2483613/range2483613/1
type: Range
persistent identity: range2483613
display id: range2483613
version: 1
direction: direction/forward
top level: BBa_K2010007
start: 1772
end: 1789
orientation: inline

pelB Tag
type: SequenceAnnotation
persistent identity: annotation2483607
display id: annotation2483607
version: 1
top level: BBa_K2010007
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K2010007/annotation2483607/range2483607/1

https://synbiohub.org/public/igem/BBa_K2010007/annotation2483607/range2483607/1
type: Range
persistent identity: range2483607
display id: range2483607
version: 1
direction: direction/forward
top level: BBa_K2010007
start: 65
end: 130
orientation: inline

Stop Codon
type: SequenceAnnotation
persistent identity: annotation2483614
display id: annotation2483614
version: 1
top level: BBa_K2010007
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K2010007/annotation2483614/range2483614/1

https://synbiohub.org/public/igem/BBa_K2010007/annotation2483614/range2483614/1
type: Range
persistent identity: range2483614
display id: range2483614
version: 1
direction: direction/forward
top level: BBa_K2010007
start: 1790
end: 1795
orientation: inline

PETase
type: SequenceAnnotation
persistent identity: annotation2483611
display id: annotation2483611
version: 1
top level: BBa_K2010007
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K2010007/annotation2483611/range2483611/1

https://synbiohub.org/public/igem/BBa_K2010007/annotation2483611/range2483611/1
type: Range
persistent identity: range2483611
display id: range2483611
version: 1
direction: direction/forward
top level: BBa_K2010007
start: 866
end: 1735
orientation: inline

GGS Linker
type: SequenceAnnotation
persistent identity: annotation2483612
display id: annotation2483612
version: 1
top level: BBa_K2010007
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K2010007/annotation2483612/range2483612/1

https://synbiohub.org/public/igem/BBa_K2010007/annotation2483612/range2483612/1
type: Range
persistent identity: range2483612
display id: range2483612
version: 1
direction: direction/forward
top level: BBa_K2010007
start: 1736
end: 1771
orientation: inline

T7 Promoter
type: SequenceAnnotation
persistent identity: annotation2483605
display id: annotation2483605
version: 1
top level: BBa_K2010007
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K2010007/annotation2483605/range2483605/1

https://synbiohub.org/public/igem/BBa_K2010007/annotation2483605/range2483605/1
type: Range
persistent identity: range2483605
display id: range2483605
version: 1
direction: direction/forward
top level: BBa_K2010007
start: 1
end: 46
orientation: inline

sfGFP
type: SequenceAnnotation
persistent identity: annotation2483609
display id: annotation2483609
version: 1
top level: BBa_K2010007
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K2010007/annotation2483609/range2483609/1

https://synbiohub.org/public/igem/BBa_K2010007/annotation2483609/range2483609/1
type: Range
persistent identity: range2483609
display id: range2483609
version: 1
direction: direction/forward
top level: BBa_K2010007
start: 137
end: 850
orientation: inline

RBS 34
type: SequenceAnnotation
persistent identity: annotation2483606
display id: annotation2483606
version: 1
top level: BBa_K2010007
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K2010007/annotation2483606/range2483606/1

https://synbiohub.org/public/igem/BBa_K2010007/annotation2483606/range2483606/1
type: Range
persistent identity: range2483606
display id: range2483606
version: 1
direction: direction/forward
top level: BBa_K2010007
start: 47
end: 58
orientation: inline

BBa_K1031623
type: ComponentDefinition
persistent identity: BBa K1031623
display id: BBa_K1031623
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: J23113-NahF-Terminator
creator: He Shuaixin
created: 2013-09-08T11:00:00Z
modified: 2015-05-08T01:08:47Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1338_
m user id: 0
owner id: 15843
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: 113-NahF-TT
mutable notes: molecular cloning
mutable provenance: synthesis Pseudomonas
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: B0015
sequence annotation: J23113
sequence annotation: NahF
sequence: https://synbiohub.org/public/igem/BBa_K1031623_sequence/1

B0015
type: SequenceAnnotation
persistent identity: annotation2361894
display id: annotation2361894
version: 1
top level: BBa_K1031623
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1031623/annotation2361894/range2361894/1

https://synbiohub.org/public/igem/BBa_K1031623/annotation2361894/range2361894/1
type: Range
persistent identity: range2361894
display id: range2361894
version: 1
direction: direction/forward
top level: BBa_K1031623
start: 1520
end: 1648
orientation: inline

J23113
type: SequenceAnnotation
persistent identity: annotation2361890
display id: annotation2361890
version: 1
top level: BBa_K1031623
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1031623/annotation2361890/range2361890/1

https://synbiohub.org/public/igem/BBa_K1031623/annotation2361890/range2361890/1
type: Range
persistent identity: range2361890
display id: range2361890
version: 1
direction: direction/forward
top level: BBa_K1031623
start: 1
end: 35
orientation: inline

NahF
type: SequenceAnnotation
persistent identity: annotation2361891
display id: annotation2361891
version: 1
top level: BBa_K1031623
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1031623/annotation2361891/range2361891/1

https://synbiohub.org/public/igem/BBa_K1031623/annotation2361891/range2361891/1
type: Range
persistent identity: range2361891
display id: range2361891
version: 1
direction: direction/forward
top level: BBa_K1031623
start: 62
end: 1513
orientation: inline

BBa_K863005
type: ComponentDefinition
persistent identity: BBa K863005
display id: BBa_K863005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: ecol laccase from E. coli with T7 promoter, RBS and His-tag
creator: Isabel Huber
created: 2012-09-17T11:00:00Z
modified: 2015-05-08T01:13:36Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1123_
m user id: 0
owner id: 12844
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: E.coli laccase ORF with T7, RBS and HIS tag
mutable notes: TEST
mutable provenance: E. coli BL21 (DE3)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: TAA
sequence annotation: ATG
sequence annotation: ecol
sequence annotation: RBS
sequence annotation: 6x HIS
sequence annotation: T7 promoter
sequence: https://synbiohub.org/public/igem/BBa_K863005_sequence/1

TAA
type: SequenceAnnotation
persistent identity: annotation2184563
display id: annotation2184563
version: 1
top level: BBa_K863005
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K863005/annotation2184563/range2184563/1

https://synbiohub.org/public/igem/BBa_K863005/annotation2184563/range2184563/1
type: Range
persistent identity: range2184563
display id: range2184563
version: 1
direction: direction/forward
top level: BBa_K863005
start: 1603
end: 1605
orientation: inline

TAA
type: SequenceAnnotation
persistent identity: annotation2184564
display id: annotation2184564
version: 1
top level: BBa_K863005
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K863005/annotation2184564/range2184564/1

https://synbiohub.org/public/igem/BBa_K863005/annotation2184564/range2184564/1
type: Range
persistent identity: range2184564
display id: range2184564
version: 1
direction: direction/forward
top level: BBa_K863005
start: 1600
end: 1602
orientation: inline

ATG
type: SequenceAnnotation
persistent identity: annotation2184562
display id: annotation2184562
version: 1
top level: BBa_K863005
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K863005/annotation2184562/range2184562/1

https://synbiohub.org/public/igem/BBa_K863005/annotation2184562/range2184562/1
type: Range
persistent identity: range2184562
display id: range2184562
version: 1
direction: direction/forward
top level: BBa_K863005
start: 34
end: 36
orientation: inline

ecol
type: SequenceAnnotation
persistent identity: annotation2184566
display id: annotation2184566
version: 1
top level: BBa_K863005
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K863005/annotation2184566/range2184566/1

https://synbiohub.org/public/igem/BBa_K863005/annotation2184566/range2184566/1
type: Range
persistent identity: range2184566
display id: range2184566
version: 1
direction: direction/forward
top level: BBa_K863005
start: 34
end: 1581
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2187301
display id: annotation2187301
version: 1
top level: BBa_K863005
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K863005/annotation2187301/range2187301/1

https://synbiohub.org/public/igem/BBa_K863005/annotation2187301/range2187301/1
type: Range
persistent identity: range2187301
display id: range2187301
version: 1
direction: direction/forward
top level: BBa_K863005
start: 25
end: 30
orientation: inline

6x HIS
type: SequenceAnnotation
persistent identity: annotation2184565
display id: annotation2184565
version: 1
top level: BBa_K863005
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K863005/annotation2184565/range2184565/1

https://synbiohub.org/public/igem/BBa_K863005/annotation2184565/range2184565/1
type: Range
persistent identity: range2184565
display id: range2184565
version: 1
direction: direction/forward
top level: BBa_K863005
start: 1582
end: 1599
orientation: inline

T7 promoter
type: SequenceAnnotation
persistent identity: annotation2184561
display id: annotation2184561
version: 1
top level: BBa_K863005
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K863005/annotation2184561/range2184561/1

https://synbiohub.org/public/igem/BBa_K863005/annotation2184561/range2184561/1
type: Range
persistent identity: range2184561
display id: range2184561
version: 1
direction: direction/forward
top level: BBa_K863005
start: 1
end: 23
orientation: inline

BBa_K863011
type: ComponentDefinition
persistent identity: BBa K863011
display id: BBa_K863011
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: tthl laccase from Thermus thermophilus
creator: Isabel Huber
created: 2012-09-17T11:00:00Z
modified: 2015-05-08T01:13:36Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1123_
m user id: 0
owner id: 12844
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: tthl (Laccase from Thermus thermophilus) coding sequence
mutable notes: test
mutable provenance: Thermus thermophilus HB27
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: tthl
sequence annotation: TAG
sequence annotation: ATG
sequence: https://synbiohub.org/public/igem/BBa_K863011_sequence/1

tthl
type: SequenceAnnotation
persistent identity: annotation2184888
display id: annotation2184888
version: 1
top level: BBa_K863011
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K863011/annotation2184888/range2184888/1

https://synbiohub.org/public/igem/BBa_K863011/annotation2184888/range2184888/1
type: Range
persistent identity: range2184888
display id: range2184888
version: 1
direction: direction/forward
top level: BBa_K863011
start: 1
end: 1389
orientation: inline

TAG
type: SequenceAnnotation
persistent identity: annotation2184889
display id: annotation2184889
version: 1
top level: BBa_K863011
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K863011/annotation2184889/range2184889/1

https://synbiohub.org/public/igem/BBa_K863011/annotation2184889/range2184889/1
type: Range
persistent identity: range2184889
display id: range2184889
version: 1
direction: direction/forward
top level: BBa_K863011
start: 1387
end: 1389
orientation: inline

ATG
type: SequenceAnnotation
persistent identity: annotation2184806
display id: annotation2184806
version: 1
top level: BBa_K863011
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K863011/annotation2184806/range2184806/1

https://synbiohub.org/public/igem/BBa_K863011/annotation2184806/range2184806/1
type: Range
persistent identity: range2184806
display id: range2184806
version: 1
direction: direction/forward
top level: BBa_K863011
start: 1
end: 3
orientation: inline

BBa_K808030
type: ComponentDefinition
persistent identity: BBa K808030
display id: BBa_K808030
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS-PhoA-His6tag-pNBEst13-Myctag-EstA
creator: Marie Burghard, Henrik Cordes, Jascha Diemer, Sven Jager, Philipp Rottmann, Rene Sahm, Arne Wehling
created: 2012-09-01T11:00:00Z
modified: 2015-05-08T01:13:26Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1065_
m user id: 0
owner id: 11792
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: RBS-PhoA-His6tag-pNBEst13-Myctag-EstA is a long name for a long protein construct. It is a fusion protein which is secreted into the peri plasma in order to be integrated into the outer membran. When the phoA signal sequence is cut off, the N-terminus is formed by our pNB-Est13. This esterase shows actitivty towards PET and is anchored onto the cell by its inactive EstA membrane anchor.
mutable notes: We got it synthesized by GENEART but added the RBS via PCR.
mutable provenance: This is no natural or even native construct. We got it synthesized by GENEART. but added the RBS via PCR.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: BBa_K808026
sequence annotation: myctag
sequence annotation: BBa_B0029
sequence annotation: BBa_K808027
sequence annotation: BBa_K808028
sequence annotation: start
sequence annotation: stop
sequence annotation: 6 histag
sequence: https://synbiohub.org/public/igem/BBa_K808030_sequence/1

BBa_K808026
type: SequenceAnnotation
persistent identity: annotation2182935
display id: annotation2182935
version: 1
top level: BBa_K808030
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K808030/annotation2182935/range2182935/1

https://synbiohub.org/public/igem/BBa_K808030/annotation2182935/range2182935/1
type: Range
persistent identity: range2182935
display id: range2182935
version: 1
direction: direction/forward
top level: BBa_K808030
start: 104
end: 1573
orientation: inline

myctag
type: SequenceAnnotation
persistent identity: annotation2182936
display id: annotation2182936
version: 1
top level: BBa_K808030
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K808030/annotation2182936/range2182936/1

https://synbiohub.org/public/igem/BBa_K808030/annotation2182936/range2182936/1
type: Range
persistent identity: range2182936
display id: range2182936
version: 1
direction: direction/forward
top level: BBa_K808030
start: 1574
end: 1603
orientation: inline

BBa_B0029
type: SequenceAnnotation
persistent identity: annotation2182926
display id: annotation2182926
version: 1
top level: BBa_K808030
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K808030/annotation2182926/range2182926/1

https://synbiohub.org/public/igem/BBa_K808030/annotation2182926/range2182926/1
type: Range
persistent identity: range2182926
display id: range2182926
version: 1
direction: direction/forward
top level: BBa_K808030
start: 2
end: 16
orientation: inline

BBa_K808027
type: SequenceAnnotation
persistent identity: annotation2182937
display id: annotation2182937
version: 1
top level: BBa_K808030
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K808030/annotation2182937/range2182937/1

https://synbiohub.org/public/igem/BBa_K808030/annotation2182937/range2182937/1
type: Range
persistent identity: range2182937
display id: range2182937
version: 1
direction: direction/forward
top level: BBa_K808030
start: 1604
end: 3037
orientation: inline

BBa_K808028
type: SequenceAnnotation
persistent identity: annotation2182934
display id: annotation2182934
version: 1
top level: BBa_K808030
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K808030/annotation2182934/range2182934/1

https://synbiohub.org/public/igem/BBa_K808030/annotation2182934/range2182934/1
type: Range
persistent identity: range2182934
display id: range2182934
version: 1
direction: direction/forward
top level: BBa_K808030
start: 23
end: 85
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2182940
display id: annotation2182940
version: 1
top level: BBa_K808030
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K808030/annotation2182940/range2182940/1

https://synbiohub.org/public/igem/BBa_K808030/annotation2182940/range2182940/1
type: Range
persistent identity: range2182940
display id: range2182940
version: 1
direction: direction/forward
top level: BBa_K808030
start: 23
end: 25
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2182939
display id: annotation2182939
version: 1
top level: BBa_K808030
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K808030/annotation2182939/range2182939/1

https://synbiohub.org/public/igem/BBa_K808030/annotation2182939/range2182939/1
type: Range
persistent identity: range2182939
display id: range2182939
version: 1
direction: direction/forward
top level: BBa_K808030
start: 3041
end: 3043
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2182938
display id: annotation2182938
version: 1
top level: BBa_K808030
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K808030/annotation2182938/range2182938/1

https://synbiohub.org/public/igem/BBa_K808030/annotation2182938/range2182938/1
type: Range
persistent identity: range2182938
display id: range2182938
version: 1
direction: direction/forward
top level: BBa_K808030
start: 3038
end: 3040
orientation: inline

6 histag
type: SequenceAnnotation
persistent identity: annotation2182933
display id: annotation2182933
version: 1
top level: BBa_K808030
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K808030/annotation2182933/range2182933/1

https://synbiohub.org/public/igem/BBa_K808030/annotation2182933/range2182933/1
type: Range
persistent identity: range2182933
display id: range2182933
version: 1
direction: direction/forward
top level: BBa_K808030
start: 86
end: 103
orientation: inline

Bt-ALDH
type: ComponentDefinition
persistent identity: BBa K398006
display id: BBa_K398006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Medium-Chain Aldehyde Dehydrogenase
creator: Mathias Voges
created: 2010-06-12T11:00:00Z
modified: 2015-05-08T01:12:22Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _506_
m user id: 0
owner id: 6320
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Aldehyde dehydrogenase from Geobacillus thermoleovorans B23.
mutable notes: Nucleotide sequence optimized for expression in E.coli.
mutable provenance: Genbank
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Bt-ALDH
sequence annotation: stop
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_K398006_sequence/1

Bt-ALDH
type: SequenceAnnotation
persistent identity: annotation2070757
display id: annotation2070757
version: 1
top level: Bt-ALDH
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K398006/annotation2070757/range2070757/1

https://synbiohub.org/public/igem/BBa_K398006/annotation2070757/range2070757/1
type: Range
persistent identity: range2070757
display id: range2070757
version: 1
direction: direction/forward
top level: Bt-ALDH
start: 1
end: 1494
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2070756
display id: annotation2070756
version: 1
top level: Bt-ALDH
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K398006/annotation2070756/range2070756/1

https://synbiohub.org/public/igem/BBa_K398006/annotation2070756/range2070756/1
type: Range
persistent identity: range2070756
display id: range2070756
version: 1
direction: direction/forward
top level: Bt-ALDH
start: 1492
end: 1494
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2070755
display id: annotation2070755
version: 1
top level: Bt-ALDH
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K398006/annotation2070755/range2070755/1

https://synbiohub.org/public/igem/BBa_K398006/annotation2070755/range2070755/1
type: Range
persistent identity: range2070755
display id: range2070755
version: 1
direction: direction/forward
top level: Bt-ALDH
start: 1
end: 3
orientation: inline

BBa_K1149006
type: ComponentDefinition
persistent identity: BBa K1149006
display id: BBa_K1149006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pelB-pulA-flag expression
creator: Margarita Kopniczky
created: 2013-09-12T11:00:00Z
modified: 2015-05-08T01:09:26Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1461_
m user id: 0
owner id: 17968
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: PUR degradation
mutable notes: E.coli optimized
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0034
component: BBa_K1149034
component: BBa_K206000
sequence annotation: BBa_K206000
sequence annotation: BBa_K1149034
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K1149006_sequence/1

BBa_B0034
type: Component
persistent identity: component2340827
display id: component2340827
version: 1
top level: BBa_K1149006
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K1149034
type: Component
persistent identity: component2340830
display id: component2340830
version: 1
top level: BBa_K1149006
access: public
definition: https://synbiohub.org/public/igem/BBa_K1149034/1

BBa_K206000
type: Component
persistent identity: component2340825
display id: component2340825
version: 1
top level: BBa_K1149006
access: public
definition: https://synbiohub.org/public/igem/BBa_K206000/1

BBa_K206000
type: SequenceAnnotation
persistent identity: annotation2340825
display id: annotation2340825
version: 1
top level: BBa_K1149006
location: https://synbiohub.org/public/igem/BBa_K1149006/annotation2340825/range2340825/1
component: BBa_K206000

https://synbiohub.org/public/igem/BBa_K1149006/annotation2340825/range2340825/1
type: Range
persistent identity: range2340825
display id: range2340825
version: 1
direction: direction/reverse
top level: BBa_K1149006
start: 1
end: 130
orientation: inline

BBa_K1149034
type: SequenceAnnotation
persistent identity: annotation2340830
display id: annotation2340830
version: 1
top level: BBa_K1149006
location: https://synbiohub.org/public/igem/BBa_K1149006/annotation2340830/range2340830/1
component: BBa_K1149034

https://synbiohub.org/public/igem/BBa_K1149006/annotation2340830/range2340830/1
type: Range
persistent identity: range2340830
display id: range2340830
version: 1
direction: direction/reverse
top level: BBa_K1149006
start: 157
end: 1605
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2340827
display id: annotation2340827
version: 1
top level: BBa_K1149006
location: https://synbiohub.org/public/igem/BBa_K1149006/annotation2340827/range2340827/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1149006/annotation2340827/range2340827/1
type: Range
persistent identity: range2340827
display id: range2340827
version: 1
direction: direction/reverse
top level: BBa_K1149006
start: 139
end: 150
orientation: inline

BBa_K137067
type: ComponentDefinition
persistent identity: BBa K137067
display id: BBa_K137067
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: katG
creator: Doug Tischer
created: 2008-07-30T11:00:00Z
modified: 2015-05-08T01:10:09Z
discontinued: false
dominant: false
experience: experience/None
group u list: _187_
m user id: 0
owner id: 3049
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This is one of the two catalases from E. coli.
mutable notes: Site directed mutagensis to remove an EcoRI site.
mutable provenance: Genomic PCR off of DH10B.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: C->T
sequence: https://synbiohub.org/public/igem/BBa_K137067_sequence/1

C->T
type: SequenceAnnotation
persistent identity: annotation1969498
display id: annotation1969498
version: 1
top level: BBa_K137067
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K137067/annotation1969498/range1969498/1

https://synbiohub.org/public/igem/BBa_K137067/annotation1969498/range1969498/1
type: Range
persistent identity: range1969498
display id: range1969498
version: 1
direction: direction/forward
top level: BBa_K137067
start: 198
end: 198
orientation: inline

BBa_K863012
type: ComponentDefinition
persistent identity: BBa K863012
display id: BBa_K863012
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: tthl laccase from T. thermophilus with constitutive promoter J23100, RBS and His-tag
creator: Isabel Huber
created: 2012-09-17T11:00:00Z
modified: 2015-05-08T01:13:36Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1123_
m user id: 0
owner id: 12844
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: tthl laccase (T. thermophilus) with constitutive promoter and HIS tag
mutable notes: test
mutable provenance: T. thermophilus HB27
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: BBa_J61101
sequence annotation: ATG
sequence annotation: J23100
sequence annotation: g (original) --> a (mutation)
sequence annotation: TAA
sequence annotation: 6 x HIS
sequence annotation: tthl
sequence: https://synbiohub.org/public/igem/BBa_K863012_sequence/1

BBa_J61101
type: SequenceAnnotation
persistent identity: annotation2185216
display id: annotation2185216
version: 1
top level: BBa_K863012
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K863012/annotation2185216/range2185216/1

https://synbiohub.org/public/igem/BBa_K863012/annotation2185216/range2185216/1
type: Range
persistent identity: range2185216
display id: range2185216
version: 1
direction: direction/forward
top level: BBa_K863012
start: 44
end: 55
orientation: inline

ATG
type: SequenceAnnotation
persistent identity: annotation2185217
display id: annotation2185217
version: 1
top level: BBa_K863012
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K863012/annotation2185217/range2185217/1

https://synbiohub.org/public/igem/BBa_K863012/annotation2185217/range2185217/1
type: Range
persistent identity: range2185217
display id: range2185217
version: 1
direction: direction/forward
top level: BBa_K863012
start: 62
end: 64
orientation: inline

J23100
type: SequenceAnnotation
persistent identity: annotation2185215
display id: annotation2185215
version: 1
top level: BBa_K863012
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K863012/annotation2185215/range2185215/1

https://synbiohub.org/public/igem/BBa_K863012/annotation2185215/range2185215/1
type: Range
persistent identity: range2185215
display id: range2185215
version: 1
direction: direction/forward
top level: BBa_K863012
start: 1
end: 35
orientation: inline

g (original) --> a (mutation)
type: SequenceAnnotation
persistent identity: annotation2185245
display id: annotation2185245
version: 1
top level: BBa_K863012
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_K863012/annotation2185245/range2185245/1

https://synbiohub.org/public/igem/BBa_K863012/annotation2185245/range2185245/1
type: Range
persistent identity: range2185245
display id: range2185245
version: 1
direction: direction/forward
top level: BBa_K863012
start: 775
end: 775
orientation: inline

TAA
type: SequenceAnnotation
persistent identity: annotation2185224
display id: annotation2185224
version: 1
top level: BBa_K863012
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K863012/annotation2185224/range2185224/1

https://synbiohub.org/public/igem/BBa_K863012/annotation2185224/range2185224/1
type: Range
persistent identity: range2185224
display id: range2185224
version: 1
direction: direction/forward
top level: BBa_K863012
start: 1469
end: 1471
orientation: inline

6 x HIS
type: SequenceAnnotation
persistent identity: annotation2185218
display id: annotation2185218
version: 1
top level: BBa_K863012
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K863012/annotation2185218/range2185218/1

https://synbiohub.org/public/igem/BBa_K863012/annotation2185218/range2185218/1
type: Range
persistent identity: range2185218
display id: range2185218
version: 1
direction: direction/forward
top level: BBa_K863012
start: 1448
end: 1465
orientation: inline

TAA
type: SequenceAnnotation
persistent identity: annotation2185223
display id: annotation2185223
version: 1
top level: BBa_K863012
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K863012/annotation2185223/range2185223/1

https://synbiohub.org/public/igem/BBa_K863012/annotation2185223/range2185223/1
type: Range
persistent identity: range2185223
display id: range2185223
version: 1
direction: direction/forward
top level: BBa_K863012
start: 1466
end: 1468
orientation: inline

tthl
type: SequenceAnnotation
persistent identity: annotation2185228
display id: annotation2185228
version: 1
top level: BBa_K863012
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K863012/annotation2185228/range2185228/1

https://synbiohub.org/public/igem/BBa_K863012/annotation2185228/range2185228/1
type: Range
persistent identity: range2185228
display id: range2185228
version: 1
direction: direction/forward
top level: BBa_K863012
start: 62
end: 1447
orientation: inline

BBa_K1031621
type: ComponentDefinition
persistent identity: BBa K1031621
display id: BBa_K1031621
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: J23105-NahF-Terminator
creator: He Shuaixin
created: 2013-09-08T11:00:00Z
modified: 2015-05-08T01:08:47Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1338_
m user id: 0
owner id: 15843
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: 105-NahF-TT
mutable notes: molecular cloning
mutable provenance: synthesis Pseudomonas
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: B0015
sequence annotation: NahF
sequence annotation: J23105
sequence: https://synbiohub.org/public/igem/BBa_K1031621_sequence/1

B0015
type: SequenceAnnotation
persistent identity: annotation2361696
display id: annotation2361696
version: 1
top level: BBa_K1031621
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1031621/annotation2361696/range2361696/1

https://synbiohub.org/public/igem/BBa_K1031621/annotation2361696/range2361696/1
type: Range
persistent identity: range2361696
display id: range2361696
version: 1
direction: direction/forward
top level: BBa_K1031621
start: 1520
end: 1648
orientation: inline

NahF
type: SequenceAnnotation
persistent identity: annotation2361695
display id: annotation2361695
version: 1
top level: BBa_K1031621
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1031621/annotation2361695/range2361695/1

https://synbiohub.org/public/igem/BBa_K1031621/annotation2361695/range2361695/1
type: Range
persistent identity: range2361695
display id: range2361695
version: 1
direction: direction/forward
top level: BBa_K1031621
start: 62
end: 1513
orientation: inline

J23105
type: SequenceAnnotation
persistent identity: annotation2361694
display id: annotation2361694
version: 1
top level: BBa_K1031621
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1031621/annotation2361694/range2361694/1

https://synbiohub.org/public/igem/BBa_K1031621/annotation2361694/range2361694/1
type: Range
persistent identity: range2361694
display id: range2361694
version: 1
direction: direction/forward
top level: BBa_K1031621
start: 1
end: 35
orientation: inline