//function/conjugation
type: Collection
persistent identity: function conjugation
display id: function_conjugation
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
owned by: james
owned by: myers
member: BBa_K109550
member: OriT-RP4
member: BBa_K808028
member: oriT/LacZ
member: BBa_K175000
member: BBa_I714031
member: BBa_K175001
member: TraJR
member: BBa_J01078
member: OriTR
member: BBa_K109551
member: BBa_J01077
member: BBa_I714030
member: TraJF
member: OriTF
member: LT
member: BBa_I714032
member: oriTr/LacZ
member: BBa_J61030

OriTR
type: ComponentDefinition
persistent identity: BBa J01003
display id: BBa_J01003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: OriT-R (Origin of transfer for the R-plasmid nic region)
creator: Golden Bear
created: 2005-10-17T11:00:00Z
modified: 2015-08-31T04:08:11Z
discontinued: false
dominant: false
experience: experience/None
group u list: _13_
m user id: 0
owner id: 395
owning group id: 13
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: OriTR, the R plasmid nic region, is where the relaxosome nicks the plasmid and conjugative transfer by R plasmid machinery begins.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Conjugation
role: SO:0000724
sequence: https://synbiohub.org/public/igem/BBa_J01003_sequence/1

OriT-RP4
type: ComponentDefinition
persistent identity: BBa K125320
display id: BBa_K125320
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: OriT-RP4 (Origin of transfer for the RP4 plasmid)
creator: Margaret Ruzicka
created: 2008-08-28T11:00:00Z
modified: 2015-05-08T01:09:45Z
discontinued: false
dominant: false
experience: experience/None
group u list: _179_
m user id: 0
owner id: 3138
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Relaxation region of self-transmissible plasmid RP4. When this part is added to a plasmid, it is transferred by RP4 through conjugation.
mutable notes: This part is similar to J01003, which has no experience and is much longer. In the process of designing and testing this part, it will be compared to J01003.
mutable provenance: RP4 NCBI accession number NC_001621
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Conjugation
role: SO:0000724
sequence: https://synbiohub.org/public/igem/BBa_K125320_sequence/1

BBa_J01077
type: ComponentDefinition
persistent identity: BBa J01077
display id: BBa_J01077
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: F plasmid (x-OriT, x-TraJ)
creator: Golden Bear
created: 2005-11-10T12:00:00Z
modified: 2015-08-31T04:08:12Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _13_
m user id: 0
owner id: 395
owning group id: 13
sample status: No part sequence
status: status/Planning
bookmark: false
mutable description: F plasmid with OriT and TraJ knocked out by lambda-red
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000155
role: partType/Plasmid

BBa_K109551
type: ComponentDefinition
persistent identity: BBa K109551
display id: BBa_K109551
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TrbA transcription factor for the Tra2 operon in incP alpha conjugation
creator: Ingrid Swanson
created: 2008-10-22T11:00:00Z
modified: 2015-05-08T01:09:08Z
discontinued: false
dominant: false
experience: experience/None
group u list: _250_
m user id: 0
owner id: 2631
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Scott to enter
mutable notes: Scott to enter
mutable provenance: Scott to enter
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K109551_sequence/1

LT
type: ComponentDefinition
persistent identity: BBa K2144101
display id: BBa_K2144101
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Vector containing Hisx6 and Sortase tag LPETGG
creator: Sigrun Stulz
created: 2016-10-13T11:00:00Z
modified: 2016-10-14T01:15:18Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2614_
m user id: 31510
owner id: 31510
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This vector is constructed to simplify the usage of the transpeptidase Sortase A for future iGEM teams. Following a BamHI restriction site we constructed a synthetic sequence consisting of three main elements: 1) A linker (GSSS) to allow for limited mobility of the attached peptides and facilitate correct folding 2) A Sortase A tag (LPETGG) that is used to conjugate the proteins to poly-glycines 3) A standard Hisx6 tag commonly used for Immobilized Metal Affinity Chromatography (IMAC) This sequence follows a BamHI restriction site which can be used by teams to clone in whichever peptide they would like to conjugate using Sortase A.
mutable notes: BamHI is a very convenient restriction enzyme for our application as its restriction site will code for a glycine and a serine, both of which are often used in linkers and tend to be unproblematic in such sequences.
mutable provenance: The plasmid was constructed using the iGEM-supplied pSB1C3 and a synthetically produced sequence.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: stop
sequence annotation: stem_loop
sequence annotation: Stop
sequence annotation: Sortase A Tag
sequence annotation: Hisx6
sequence annotation: BBa_R0010
sequence annotation: -10
sequence annotation: BBa_B0034
sequence annotation: BBa_B0010
sequence annotation: CmR
sequence annotation: polya
sequence annotation: -35
sequence annotation: conserved
sequence annotation: T7 TE
sequence annotation: CAP
sequence annotation: ori
sequence annotation: barcode
sequence annotation: start
sequence annotation: BBa_B0012
sequence annotation: LacI
sequence: https://synbiohub.org/public/igem/BBa_K2144101_sequence/1

stop
type: SequenceAnnotation
persistent identity: annotation2509050
display id: annotation2509050
version: 1
top level: LT
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K2144101/annotation2509050/range2509050/1

https://synbiohub.org/public/igem/BBa_K2144101/annotation2509050/range2509050/1
type: Range
persistent identity: range2509050
display id: range2509050
version: 1
direction: direction/forward
top level: LT
start: 1062
end: 1062
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation2509046
display id: annotation2509046
version: 1
top level: LT
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K2144101/annotation2509046/range2509046/1

https://synbiohub.org/public/igem/BBa_K2144101/annotation2509046/range2509046/1
type: Range
persistent identity: range2509046
display id: range2509046
version: 1
direction: direction/forward
top level: LT
start: 952
end: 995
orientation: inline

Stop
type: SequenceAnnotation
persistent identity: annotation2509054
display id: annotation2509054
version: 1
top level: LT
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K2144101/annotation2509054/range2509054/1

https://synbiohub.org/public/igem/BBa_K2144101/annotation2509054/range2509054/1
type: Range
persistent identity: range2509054
display id: range2509054
version: 1
direction: direction/forward
top level: LT
start: 2095
end: 2097
orientation: inline

Sortase A Tag
type: SequenceAnnotation
persistent identity: annotation2509053
display id: annotation2509053
version: 1
top level: LT
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K2144101/annotation2509053/range2509053/1

https://synbiohub.org/public/igem/BBa_K2144101/annotation2509053/range2509053/1
type: Range
persistent identity: range2509053
display id: range2509053
version: 1
direction: direction/forward
top level: LT
start: 2059
end: 2076
orientation: inline

Hisx6
type: SequenceAnnotation
persistent identity: annotation2509052
display id: annotation2509052
version: 1
top level: LT
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K2144101/annotation2509052/range2509052/1

https://synbiohub.org/public/igem/BBa_K2144101/annotation2509052/range2509052/1
type: Range
persistent identity: range2509052
display id: range2509052
version: 1
direction: direction/forward
top level: LT
start: 2077
end: 2094
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation2509035
display id: annotation2509035
version: 1
top level: LT
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K2144101/annotation2509035/range2509035/1

https://synbiohub.org/public/igem/BBa_K2144101/annotation2509035/range2509035/1
type: Range
persistent identity: range2509035
display id: range2509035
version: 1
direction: direction/forward
top level: LT
start: 1
end: 200
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2509038
display id: annotation2509038
version: 1
top level: LT
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K2144101/annotation2509038/range2509038/1

https://synbiohub.org/public/igem/BBa_K2144101/annotation2509038/range2509038/1
type: Range
persistent identity: range2509038
display id: range2509038
version: 1
direction: direction/forward
top level: LT
start: 161
end: 166
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2509041
display id: annotation2509041
version: 1
top level: LT
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K2144101/annotation2509041/range2509041/1

https://synbiohub.org/public/igem/BBa_K2144101/annotation2509041/range2509041/1
type: Range
persistent identity: range2509041
display id: range2509041
version: 1
direction: direction/forward
top level: LT
start: 209
end: 220
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2509045
display id: annotation2509045
version: 1
top level: LT
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K2144101/annotation2509045/range2509045/1

https://synbiohub.org/public/igem/BBa_K2144101/annotation2509045/range2509045/1
type: Range
persistent identity: range2509045
display id: range2509045
version: 1
direction: direction/forward
top level: LT
start: 941
end: 1020
orientation: inline

CmR
type: SequenceAnnotation
persistent identity: annotation2509051
display id: annotation2509051
version: 1
top level: LT
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K2144101/annotation2509051/range2509051/1

https://synbiohub.org/public/igem/BBa_K2144101/annotation2509051/range2509051/1
type: Range
persistent identity: range2509051
display id: range2509051
version: 1
direction: direction/forward
top level: LT
start: 1140
end: 1799
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation2509049
display id: annotation2509049
version: 1
top level: LT
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_K2144101/annotation2509049/range2509049/1

https://synbiohub.org/public/igem/BBa_K2144101/annotation2509049/range2509049/1
type: Range
persistent identity: range2509049
display id: range2509049
version: 1
direction: direction/forward
top level: LT
start: 1056
end: 1069
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2509037
display id: annotation2509037
version: 1
top level: LT
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K2144101/annotation2509037/range2509037/1

https://synbiohub.org/public/igem/BBa_K2144101/annotation2509037/range2509037/1
type: Range
persistent identity: range2509037
display id: range2509037
version: 1
direction: direction/forward
top level: LT
start: 137
end: 142
orientation: inline

conserved
type: SequenceAnnotation
persistent identity: annotation2509042
display id: annotation2509042
version: 1
top level: LT
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K2144101/annotation2509042/range2509042/1

https://synbiohub.org/public/igem/BBa_K2144101/annotation2509042/range2509042/1
type: Range
persistent identity: range2509042
display id: range2509042
version: 1
direction: direction/forward
top level: LT
start: 213
end: 216
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation2509048
display id: annotation2509048
version: 1
top level: LT
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K2144101/annotation2509048/range2509048/1

https://synbiohub.org/public/igem/BBa_K2144101/annotation2509048/range2509048/1
type: Range
persistent identity: range2509048
display id: range2509048
version: 1
direction: direction/forward
top level: LT
start: 1036
end: 1055
orientation: inline

CAP
type: SequenceAnnotation
persistent identity: annotation2509036
display id: annotation2509036
version: 1
top level: LT
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K2144101/annotation2509036/range2509036/1

https://synbiohub.org/public/igem/BBa_K2144101/annotation2509036/range2509036/1
type: Range
persistent identity: range2509036
display id: range2509036
version: 1
direction: direction/forward
top level: LT
start: 89
end: 126
orientation: inline

ori
type: SequenceAnnotation
persistent identity: annotation2509043
display id: annotation2509043
version: 1
top level: LT
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K2144101/annotation2509043/range2509043/1

https://synbiohub.org/public/igem/BBa_K2144101/annotation2509043/range2509043/1
type: Range
persistent identity: range2509043
display id: range2509043
version: 1
direction: direction/forward
top level: LT
start: 254
end: 842
orientation: inline

barcode
type: SequenceAnnotation
persistent identity: annotation2509044
display id: annotation2509044
version: 1
top level: LT
role: SO:0000807
role: feature/barcode
location: https://synbiohub.org/public/igem/BBa_K2144101/annotation2509044/range2509044/1

https://synbiohub.org/public/igem/BBa_K2144101/annotation2509044/range2509044/1
type: Range
persistent identity: range2509044
display id: range2509044
version: 1
direction: direction/forward
top level: LT
start: 908
end: 932
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2509040
display id: annotation2509040
version: 1
top level: LT
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K2144101/annotation2509040/range2509040/1

https://synbiohub.org/public/igem/BBa_K2144101/annotation2509040/range2509040/1
type: Range
persistent identity: range2509040
display id: range2509040
version: 1
direction: direction/forward
top level: LT
start: 173
end: 173
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2509047
display id: annotation2509047
version: 1
top level: LT
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K2144101/annotation2509047/range2509047/1

https://synbiohub.org/public/igem/BBa_K2144101/annotation2509047/range2509047/1
type: Range
persistent identity: range2509047
display id: range2509047
version: 1
direction: direction/forward
top level: LT
start: 1029
end: 1069
orientation: inline

LacI
type: SequenceAnnotation
persistent identity: annotation2509039
display id: annotation2509039
version: 1
top level: LT
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K2144101/annotation2509039/range2509039/1

https://synbiohub.org/public/igem/BBa_K2144101/annotation2509039/range2509039/1
type: Range
persistent identity: range2509039
display id: range2509039
version: 1
direction: direction/forward
top level: LT
start: 166
end: 200
orientation: inline

BBa_K109550
type: ComponentDefinition
persistent identity: BBa K109550
display id: BBa_K109550
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Bacterial conjugative regulator KorA
creator: Ingrid Swanson
created: 2008-10-22T11:00:00Z
modified: 2015-05-08T01:09:08Z
discontinued: false
dominant: false
group u list: _250_
m user id: 0
owner id: 2631
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Detailed description to be entered by Scott
mutable notes: Scott to enter
mutable provenance: IncPa E. coli conjugative plasmid
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K109550_sequence/1

BBa_I714031
type: ComponentDefinition
persistent identity: BBa I714031
display id: BBa_I714031
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: OriT-R (Origin of Transfer for the R751-plasmid nic region)
creator: Mingzhi Qu
created: 2007-10-20T11:00:00Z
modified: 2015-08-31T04:07:47Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _142_
m user id: 0
owner id: 2248
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: OriT-R751 (Origin of Transfer for the R751-type conjugative plasmid) is the nic region, where the relaxosome nicks the plasmid and conjugative transfer by R751 plasmid machinery begins via rolling circle replication.
mutable provenance: Enterobacter aerogenes plasmid R751 NCBI:NC_001735
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Conjugation
role: SO:0000724
sequence: https://synbiohub.org/public/igem/BBa_I714031_sequence/1

oriT/LacZ
type: ComponentDefinition
persistent identity: BBa K125360
display id: BBa_K125360
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: oriT with lac promoter and lacZ
creator: Margaret Ruzicka
created: 2008-10-27T12:00:00Z
modified: 2015-05-08T01:09:45Z
discontinued: false
dominant: false
experience: experience/None
group u list: _179_
m user id: 0
owner id: 3138
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part was constructed to compare the oriTr, which is currently available in the registry to oriT which was recently designed to perform the same function. The oriTr was added to LacZ part so that conjugation could be monitored by blue/white selection.
mutable notes: The oriT was synthesized and J33207 was extracted from the registry.
mutable provenance: The oriT sequence was taken from the self-transmissible plasmid RP4. LacZ is available as a part in the registry.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Conjugation
role: SO:0000724
component: BBa_J33207
component: BBa_K125320
sequence annotation: BBa_J33207
sequence annotation: BBa_K125320
sequence: https://synbiohub.org/public/igem/BBa_K125360_sequence/1

BBa_J33207
type: Component
persistent identity: component1992571
display id: component1992571
version: 1
top level: oriT/LacZ
access: public
definition: https://synbiohub.org/public/igem/BBa_J33207/1

BBa_K125320
type: Component
persistent identity: component1992572
display id: component1992572
version: 1
top level: oriT/LacZ
access: public
definition: OriT-RP4

BBa_J33207
type: SequenceAnnotation
persistent identity: annotation1992571
display id: annotation1992571
version: 1
top level: oriT/LacZ
location: https://synbiohub.org/public/igem/BBa_K125360/annotation1992571/range1992571/1
component: BBa_J33207

https://synbiohub.org/public/igem/BBa_K125360/annotation1992571/range1992571/1
type: Range
persistent identity: range1992571
display id: range1992571
version: 1
direction: direction/reverse
top level: oriT/LacZ
start: 1
end: 600
orientation: inline

BBa_K125320
type: SequenceAnnotation
persistent identity: annotation1992572
display id: annotation1992572
version: 1
top level: oriT/LacZ
location: https://synbiohub.org/public/igem/BBa_K125360/annotation1992572/range1992572/1
component: BBa_K125320

https://synbiohub.org/public/igem/BBa_K125360/annotation1992572/range1992572/1
type: Range
persistent identity: range1992572
display id: range1992572
version: 1
direction: direction/reverse
top level: oriT/LacZ
start: 609
end: 707
orientation: inline

oriTr/LacZ
type: ComponentDefinition
persistent identity: BBa K125350
display id: BBa_K125350
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: oriTr with lac promoter and lacZ
creator: Margaret Ruzicka
created: 2008-10-27T12:00:00Z
modified: 2015-05-08T01:09:45Z
discontinued: false
dominant: false
experience: experience/None
group u list: _179_
m user id: 0
owner id: 3138
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part was constructed to compare the oriTr, which is currently available in the registry to oriT which was recently designed to perform the same function. The oriTr was added to LacZ part so that conjugation could be monitored by blue/white selection.
mutable notes: This part will be tested by blue/white screening.
mutable provenance: This part was built from oriTr and LacZ.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Conjugation
role: SO:0000724
component: BBa_J33207
component: BBa_J01003
sequence annotation: BBa_J01003
sequence annotation: BBa_J33207
sequence: https://synbiohub.org/public/igem/BBa_K125350_sequence/1

BBa_J33207
type: Component
persistent identity: component1992562
display id: component1992562
version: 1
top level: oriTr/LacZ
access: public
definition: https://synbiohub.org/public/igem/BBa_J33207/1

BBa_J01003
type: Component
persistent identity: component1992563
display id: component1992563
version: 1
top level: oriTr/LacZ
access: public
definition: OriTR

BBa_J01003
type: SequenceAnnotation
persistent identity: annotation1992563
display id: annotation1992563
version: 1
top level: oriTr/LacZ
location: https://synbiohub.org/public/igem/BBa_K125350/annotation1992563/range1992563/1
component: BBa_J01003

https://synbiohub.org/public/igem/BBa_K125350/annotation1992563/range1992563/1
type: Range
persistent identity: range1992563
display id: range1992563
version: 1
direction: direction/reverse
top level: oriTr/LacZ
start: 609
end: 979
orientation: inline

BBa_J33207
type: SequenceAnnotation
persistent identity: annotation1992562
display id: annotation1992562
version: 1
top level: oriTr/LacZ
location: https://synbiohub.org/public/igem/BBa_K125350/annotation1992562/range1992562/1
component: BBa_J33207

https://synbiohub.org/public/igem/BBa_K125350/annotation1992562/range1992562/1
type: Range
persistent identity: range1992562
display id: range1992562
version: 1
direction: direction/reverse
top level: oriTr/LacZ
start: 1
end: 600
orientation: inline

TraJR
type: ComponentDefinition
persistent identity: BBa J01001
display id: BBa_J01001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TraJr protein (controls conjugative transfer in RP4 plasmid)
creator: Berkeley iGEM 2005
created: 2005-10-17T11:00:00Z
modified: 2015-08-31T04:08:11Z
discontinued: false
dominant: false
experience: experience/None
group u list: _13_
m user id: 0
owner id: 395
owning group id: 13
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: TraJR controls conjugative transfer in R plasmid
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: TraJr
sequence: https://synbiohub.org/public/igem/BBa_J01001_sequence/1

TraJr
type: SequenceAnnotation
persistent identity: annotation1891611
display id: annotation1891611
version: 1
top level: TraJR
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J01001/annotation1891611/range1891611/1

https://synbiohub.org/public/igem/BBa_J01001/annotation1891611/range1891611/1
type: Range
persistent identity: range1891611
display id: range1891611
version: 1
direction: direction/forward
top level: TraJR
start: 1
end: 378
orientation: inline

BBa_J01078
type: ComponentDefinition
persistent identity: BBa J01078
display id: BBa_J01078
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: R plasmid (x-OriT, x-TraJ)
creator: Golden Bear
created: 2005-11-10T12:00:00Z
modified: 2015-08-31T04:08:12Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _13_
m user id: 0
owner id: 395
owning group id: 13
sample status: No part sequence
status: status/Planning
bookmark: false
mutable description: R plasmid with OriT and TraJ knocked out by lambda-red
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000155
role: partType/Plasmid

BBa_K175001
type: ComponentDefinition
persistent identity: BBa K175001
display id: BBa_K175001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: trbK (entry exclusion) of IncP beta R751 plasmid
creator: Calin Plesa
created: 2009-07-05T11:00:00Z
modified: 2015-05-08T01:10:59Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _280_
m user id: 0
owner id: 4286
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This part encodes for trbK, a small 75 amino acid cytoplasmic membrane lipoprotein which handles the entry exclusion in the R751 plasmid. Expression of this part in a cell without R751 will significantly reduce the transfer frequency from R751 donors. This is designed to prevent redundant transfers from occurring. Note that the presence of entry exclusion has been linked to the prevention of lethal zygosis. If the entry exclusion (Eex) index is defined as the transfer frequency for receiver cells not containing the trbK protein divided by the transfer frequency for receiver cells containing the trbK protein than for R751 Eex index = 44,000 (See Table 5 of Haase, J., Kalkum, M. & Lanka, E. TrbK, a small cytoplasmic membrane lipoprotein, functions in entry exclusion of the IncP alpha plasmid RP4. J. Bacteriol. 178, 6720???6729 (1996).[http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=178567])
mutable notes: None
mutable provenance: This sequence can be found at http://www.ncbi.nlm.nih.gov/protein/10955220
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: trbK
sequence: https://synbiohub.org/public/igem/BBa_K175001_sequence/1

trbK
type: SequenceAnnotation
persistent identity: annotation2009448
display id: annotation2009448
version: 1
top level: BBa_K175001
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K175001/annotation2009448/range2009448/1

https://synbiohub.org/public/igem/BBa_K175001/annotation2009448/range2009448/1
type: Range
persistent identity: range2009448
display id: range2009448
version: 1
direction: direction/forward
top level: BBa_K175001
start: 1
end: 231
orientation: inline

OriTF
type: ComponentDefinition
persistent identity: BBa J01002
display id: BBa_J01002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: OriT-F (Origin of Transfer for the F-plasmid nic region)
creator: Golden Bear
created: 2005-10-17T11:00:00Z
modified: 2015-08-31T04:08:11Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _13_
m user id: 0
owner id: 395
owning group id: 13
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: OriTf (Origin of Transfer for the F-type conjugative plasmid) is the nic region, is where the relaxosome nicks the plasmid and conjugative transfer by F plasmid machinery begins via rolling circle replication. This particular plasmid has been derived from a F-type plasmid donated by Professor Laura Frost University of Alberta. NOTE: OriTf is NOT the same as the host cell's native Origin of Transfer (this is a separate site within the bacterial host's main genome).
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Conjugation
role: SO:0000724
sequence: https://synbiohub.org/public/igem/BBa_J01002_sequence/1

BBa_I714032
type: ComponentDefinition
persistent identity: BBa I714032
display id: BBa_I714032
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: OriT-S (Origin of Transfer for the pSC101-plasmid nic region)
creator: Mingzhi Qu
created: 2007-10-20T11:00:00Z
modified: 2015-08-31T04:07:47Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _142_
m user id: 0
owner id: 2248
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: OriT-S (Origin of Transfer for the pSC101-type conjugative plasmid) is the nic region, where the relaxosome nicks the plasmid and conjugative transfer by pSC101 plasmid machinery begins via rolling circle replication.
mutable provenance: pSC101 NCBI:NC_002056
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Conjugation
role: SO:0000724
sequence: https://synbiohub.org/public/igem/BBa_I714032_sequence/1

BBa_K175000
type: ComponentDefinition
persistent identity: BBa K175000
display id: BBa_K175000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: trbC (pilin) of IncP beta R751 plasmid
creator: Calin Plesa
created: 2009-07-04T11:00:00Z
modified: 2015-05-08T01:10:58Z
discontinued: false
dominant: false
group u list: _280_
m user id: 0
owner id: 4286
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: The relaxase / helicase TraI of the IncP beta plasmid R751. Essential of conjugative transfer. This part nicks oriT. It should be placed on a message plasmid along with oriT, and will enable transfer of the message plasmid in the presence of a modified R751 helper plasmid such as BBa_I714037.
mutable notes: None
mutable provenance: This part comes from http://www.ncbi.nlm.nih.gov/nuccore/70907607?from=48797&to=51040&report=gbwithparts and corresponds to range 48797..51040 of the R751 sequence.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: trbC
sequence: https://synbiohub.org/public/igem/BBa_K175000_sequence/1

trbC
type: SequenceAnnotation
persistent identity: annotation2008011
display id: annotation2008011
version: 1
top level: BBa_K175000
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K175000/annotation2008011/range2008011/1

https://synbiohub.org/public/igem/BBa_K175000/annotation2008011/range2008011/1
type: Range
persistent identity: range2008011
display id: range2008011
version: 1
direction: direction/forward
top level: BBa_K175000
start: 1
end: 468
orientation: inline

BBa_J61030
type: ComponentDefinition
persistent identity: BBa J61030
display id: BBa_J61030
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: [OriTr][R6K]
creator: John Anderson
created: 2006-09-20T11:00:00Z
modified: 2015-08-31T02:02:59Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Intermediate of the oriTr (RP4 origin of transfer) and R6K (conditional origin of replication) pSB1A2-Bca9009
mutable notes: N/A
mutable provenance: standard biobrick assembly
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_J61001
component: BBa_J01003
sequence annotation: BBa_J01003
sequence annotation: BBa_J61001
sequence: https://synbiohub.org/public/igem/BBa_J61030_sequence/1

BBa_J61001
type: Component
persistent identity: component1902259
display id: component1902259
version: 1
top level: BBa_J61030
access: public
definition: https://synbiohub.org/public/igem/BBa_J61001/1

BBa_J01003
type: Component
persistent identity: component1902257
display id: component1902257
version: 1
top level: BBa_J61030
access: public
definition: OriTR

BBa_J01003
type: SequenceAnnotation
persistent identity: annotation1902257
display id: annotation1902257
version: 1
top level: BBa_J61030
location: https://synbiohub.org/public/igem/BBa_J61030/annotation1902257/range1902257/1
component: BBa_J01003

https://synbiohub.org/public/igem/BBa_J61030/annotation1902257/range1902257/1
type: Range
persistent identity: range1902257
display id: range1902257
version: 1
direction: direction/reverse
top level: BBa_J61030
start: 1
end: 371
orientation: inline

BBa_J61001
type: SequenceAnnotation
persistent identity: annotation1902259
display id: annotation1902259
version: 1
top level: BBa_J61030
location: https://synbiohub.org/public/igem/BBa_J61030/annotation1902259/range1902259/1
component: BBa_J61001

https://synbiohub.org/public/igem/BBa_J61030/annotation1902259/range1902259/1
type: Range
persistent identity: range1902259
display id: range1902259
version: 1
direction: direction/reverse
top level: BBa_J61030
start: 380
end: 785
orientation: inline

BBa_K808028
type: ComponentDefinition
persistent identity: BBa K808028
display id: BBa_K808028
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PhoA: signal sequence for peri plasmic expression
creator: Marie Burghard, Jascha Diemer, Adrian Eilingsfeld, Philipp Rottmann, Rene Sahm, Arne Wehling
created: 2012-09-01T11:00:00Z
modified: 2015-05-08T01:13:26Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1065_
m user id: 0
owner id: 11792
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: PhoA is an N-terminal signal sequence for expression into the peri plasma. It derives from the native phosphatase A of E.coli and is similar to pelB. We used it to express our fusion protein (BBa_K808030)
mutable notes: As an signal peptide, our phoA has no stop codons and needs to be fused at the N-Terminus.
mutable provenance: Our phoA BioBrick is derived from the pEST100 vector which was used in the following publication: Becker, S., Theile, S., Heppeler, N., Michalczyk, A., Wentzel, A., Wilhelm, S., Jaeger, K.-E., et al. (2005). A generic system for the Escherichia coli cell-surface display of lipolytic enzymes. FEBS letters, 579(5), 1177???82. doi:10.1016/j.febslet.2004.12.087
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Signalling
role: SO:0000804
sequence: https://synbiohub.org/public/igem/BBa_K808028_sequence/1

TraJF
type: ComponentDefinition
persistent identity: BBa J01000
display id: BBa_J01000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TraJF controls conjugative transfer in F plasmid
creator: Berkeley iGEM 2005
created: 2005-10-17T11:00:00Z
modified: 2015-08-31T04:08:11Z
discontinued: false
dominant: false
experience: experience/None
group u list: _13_
m user id: 0
owner id: 395
owning group id: 13
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: TraJF controls conjugative transfer in F plasmid
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_J01000_sequence/1

BBa_I714030
type: ComponentDefinition
persistent identity: BBa I714030
display id: BBa_I714030
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: OriT-F (Origin of Transfer for the F-plasmid nic region)
creator: Mingzhi Qu
created: 2007-10-19T11:00:00Z
modified: 2015-08-31T04:07:47Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _142_
m user id: 0
owner id: 2248
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: OriT-F (Origin of Transfer for the F-type conjugative plasmid) is the nic region, where the relaxosome nicks the plasmid and conjugative transfer by F plasmid machinery begins via rolling circle replication.
mutable notes: This OriT-F is the same as the host cell's native Origin of Transfer, Please compare the sequence with J01002.
mutable provenance: Origin-of-transfer nic region excised from Plasmid F genomic DNA.(NCBI:AP001918)
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Conjugation
role: SO:0000724
sequence: https://synbiohub.org/public/igem/BBa_I714030_sequence/1