//function/cellsignalling/rhlr
type: Collection
persistent identity: function cellsignalling rhlr
display id: function_cellsignalling_rhlr
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
owned by: james
owned by: myers
member: https://synbiohub.org/public/igem/BBa_J06403/1
member: https://synbiohub.org/public/igem/BBa_I14017/1
member: https://synbiohub.org/public/igem/BBa_R0071/1

//function/cellsignalling/luxr
type: Collection
persistent identity: function cellsignalling luxr
display id: function_cellsignalling_luxr
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
owned by: james
owned by: myers
member: https://synbiohub.org/public/igem/BBa_K266005/1
member: https://synbiohub.org/public/igem/BBa_J102001/1
member: https://synbiohub.org/public/igem/BBa_I751502/1
member: https://synbiohub.org/public/igem/BBa_R0061/1
member: https://synbiohub.org/public/igem/BBa_K091157/1
member: https://synbiohub.org/public/igem/BBa_I739105/1
member: https://synbiohub.org/public/igem/BBa_K266006/1
member: https://synbiohub.org/public/igem/BBa_K1520515/1
member: https://synbiohub.org/public/igem/BBa_R0062/1
member: https://synbiohub.org/public/igem/BBa_R0063/1
member: https://synbiohub.org/public/igem/BBa_J64712/1
member: https://synbiohub.org/public/igem/BBa_K091156/1
member: https://synbiohub.org/public/igem/BBa_K658006/1
member: https://synbiohub.org/public/igem/BBa_K091107/1
member: https://synbiohub.org/public/igem/BBa_R1062/1
member: https://synbiohub.org/public/igem/BBa_J64067/1
member: https://synbiohub.org/public/igem/BBa_K1520516/1
member: https://synbiohub.org/public/igem/BBa_K266007/1
member: https://synbiohub.org/public/igem/BBa_K658007/1
member: https://synbiohub.org/public/igem/BBa_I1051/1
member: https://synbiohub.org/public/igem/BBa_K091146/1
member: https://synbiohub.org/public/igem/BBa_K658008/1
member: https://synbiohub.org/public/igem/BBa_I751501/1
member: https://synbiohub.org/public/igem/BBa_K145150/1
member: https://synbiohub.org/public/igem/BBa_K1520010/1

//function/cellsignalling/cinr
type: Collection
persistent identity: function cellsignalling cinr
display id: function_cellsignalling_cinr
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
owned by: james
owned by: myers
member: https://synbiohub.org/public/igem/BBa_R0078/1
member: https://synbiohub.org/public/igem/BBa_I761011/1

//function/cellsignalling/lasr
type: Collection
persistent identity: function cellsignalling lasr
display id: function_cellsignalling_lasr
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
owned by: james
owned by: myers
member: https://synbiohub.org/public/igem/BBa_K266005/1
member: https://synbiohub.org/public/igem/BBa_K091157/1
member: https://synbiohub.org/public/igem/BBa_K266006/1
member: https://synbiohub.org/public/igem/BBa_K091117/1
member: https://synbiohub.org/public/igem/BBa_K091146/1
member: https://synbiohub.org/public/igem/BBa_R0079/1
member: https://synbiohub.org/public/igem/BBa_J64712/1
member: https://synbiohub.org/public/igem/BBa_K091143/1
member: https://synbiohub.org/public/igem/BBa_K266007/1
member: https://synbiohub.org/public/igem/BBa_I14016/1
member: https://synbiohub.org/public/igem/BBa_I14015/1
member: https://synbiohub.org/public/igem/BBa_K266000/1

//function/cellsignalling/lasi
type: Collection
persistent identity: function cellsignalling lasi
display id: function_cellsignalling_lasi
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
owned by: james
owned by: myers
member: https://synbiohub.org/public/igem/BBa_J64010/1

//function/cellsignalling
type: Collection
persistent identity: function cellsignalling
display id: function_cellsignalling
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
owned by: james
owned by: myers
member: rhII
member: BBa_I13202
member: BBa_F1610
member: BBa_K082020
member: BBa_I13274
member: BBa_J37033
member: BBa_F2622
member: BBa_K1520022
member: LuxO D47A
member: BBa_K574009
member: BBa_K081015
member: BBa_K091138
member: BBa_K081009
member: LacIM
member: BBa_I13018
member: BBa_K091203
member: BBa_K1520511
member: BBa_K1897003
member: BBa_J06201
member: BBa_K081020
member: BBa_I13035
member: BBa_I13220
member: BBa_K1897002
member: BBa_K1520510
member: BBa_I13262
member: BBa_K082032
member: BBa_F2621
member: BBa_I0460
member: BBa_I1468
member: BBa_K1631022
member: BBa_I13273
member: BBa_J07045
member: BBa_I13203
member: BBa_I726031
member: BBa_K1520021
member: BBa_K990001
member: BBa_I726061
member: BBa_K082008
member: BBa_K082021
member: luxr
member: LuxR
member: BBa_K546003
member: Pgad
member: BBa_K1520512
member: BBa_I1466
member: BBa_I0426
member: rhIR
member: BBa_K211002
member: BBa_K546001
member: BBa_I751350
member: BBa_K819005
member: BBa_I13207
member: LuxOU
member: BBa_I0424
member: BBa_K1631025
member: BBa_K1520011
member: BBa_J06002
member: BBa_J13040
member: BBa_K1631024
member: //function/cellsignalling/lasi
member: BBa_K094103
member: rhlR
member: BBa_I13261
member: BBa_K546000
member: BBa_K081019
member: QS->RFP
member: lasR
member: BBa_I13208
member: BBa_K082029
member: //function/cellsignalling/cinr
member: BBa_I732923

//function/cellsignalling/rhll
type: Collection
persistent identity: function cellsignalling rhll
display id: function_cellsignalling_rhll
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
owned by: james
owned by: myers
member: https://synbiohub.org/public/igem/BBa_J64000/1

//function/cellsignalling/agra
type: Collection
persistent identity: function cellsignalling agra
display id: function_cellsignalling_agra
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
owned by: james
owned by: myers
member: https://synbiohub.org/public/igem/BBa_I746104/1

LuxPQ
type: ComponentDefinition
persistent identity: BBa K131015
display id: BBa_K131015
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LuxPQ from Vibrio harveyi
creator: Thane Kubik
created: 2008-09-27T11:00:00Z
modified: 2015-05-08T01:09:47Z
discontinued: false
dominant: true
experience: experience/None
group u list: _259_
m user id: 0
owner id: 3190
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: LuxPQ from Vibrio harveyi LuxP is a ABC-type sugar transport system periplasmic protein (Protein ID = YP_001447483.1). LuxQ is a histidine kinase (Protein ID = YP_001447484.1).
mutable notes: LuxP and LuxQ are found in the operon, thus we decided to keep them together. We kept natural RBS in front of LuxP, as well as the intrinsic terminator. Silent mutations were performed on a EcoRI site located within LuxP, EcorI site within LuxQ, and XbaI site within LuxQ.
mutable provenance: GenBank Accession: NC_009784 Vibrio harveyi ATCC BAA-1116 chromosome II, REGION: 579654..580751. Neiditch MB, Federle MJ, Pompeani AJ, Kelly RC, Swem DL, Jeffrey PD, Bassler BL, Hughson FM. Ligand-induced asymmetry in histidine sensor kinase complex regulates quorum sensing. Cell. 2006 Sep 22;126(6):1095-108. Neiditch MB, Federle MJ, Pompeani AJ, Kelly RC, Swem DL, Jeffrey PD, Bassler BL, Hughson FM.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
sequence annotation: Silent mutation to remove XbaI site
sequence annotation: LuxQ
sequence annotation: Start of LuxP
sequence annotation: Stop for LuxQ
sequence annotation: Silent mutation to remove EcoRI site
sequence annotation: Stop for LuxP
sequence annotation: LuxP
sequence annotation: Start of LuxQ
sequence: https://synbiohub.org/public/igem/BBa_K131015_sequence/1

Silent mutation to remove XbaI site
type: SequenceAnnotation
persistent identity: annotation1976854
display id: annotation1976854
version: 1
top level: LuxPQ
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_K131015/annotation1976854/range1976854/1

https://synbiohub.org/public/igem/BBa_K131015/annotation1976854/range1976854/1
type: Range
persistent identity: range1976854
display id: range1976854
version: 1
direction: direction/forward
top level: LuxPQ
start: 1618
end: 1618
orientation: inline

LuxQ
type: SequenceAnnotation
persistent identity: annotation1976851
display id: annotation1976851
version: 1
top level: LuxPQ
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K131015/annotation1976851/range1976851/1

https://synbiohub.org/public/igem/BBa_K131015/annotation1976851/range1976851/1
type: Range
persistent identity: range1976851
display id: range1976851
version: 1
direction: direction/forward
top level: LuxPQ
start: 1127
end: 3706
orientation: inline

Start of LuxP
type: SequenceAnnotation
persistent identity: annotation1976844
display id: annotation1976844
version: 1
top level: LuxPQ
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K131015/annotation1976844/range1976844/1

https://synbiohub.org/public/igem/BBa_K131015/annotation1976844/range1976844/1
type: Range
persistent identity: range1976844
display id: range1976844
version: 1
direction: direction/forward
top level: LuxPQ
start: 30
end: 32
orientation: inline

Stop for LuxQ
type: SequenceAnnotation
persistent identity: annotation1976847
display id: annotation1976847
version: 1
top level: LuxPQ
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K131015/annotation1976847/range1976847/1

https://synbiohub.org/public/igem/BBa_K131015/annotation1976847/range1976847/1
type: Range
persistent identity: range1976847
display id: range1976847
version: 1
direction: direction/forward
top level: LuxPQ
start: 3704
end: 3706
orientation: inline

Silent mutation to remove EcoRI site
type: SequenceAnnotation
persistent identity: annotation1976852
display id: annotation1976852
version: 1
top level: LuxPQ
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_K131015/annotation1976852/range1976852/1

https://synbiohub.org/public/igem/BBa_K131015/annotation1976852/range1976852/1
type: Range
persistent identity: range1976852
display id: range1976852
version: 1
direction: direction/forward
top level: LuxPQ
start: 599
end: 599
orientation: inline

Stop for LuxP
type: SequenceAnnotation
persistent identity: annotation1976845
display id: annotation1976845
version: 1
top level: LuxPQ
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K131015/annotation1976845/range1976845/1

https://synbiohub.org/public/igem/BBa_K131015/annotation1976845/range1976845/1
type: Range
persistent identity: range1976845
display id: range1976845
version: 1
direction: direction/forward
top level: LuxPQ
start: 1125
end: 1127
orientation: inline

Silent mutation to remove EcoRI site
type: SequenceAnnotation
persistent identity: annotation1976853
display id: annotation1976853
version: 1
top level: LuxPQ
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_K131015/annotation1976853/range1976853/1

https://synbiohub.org/public/igem/BBa_K131015/annotation1976853/range1976853/1
type: Range
persistent identity: range1976853
display id: range1976853
version: 1
direction: direction/forward
top level: LuxPQ
start: 1465
end: 1465
orientation: inline

LuxP
type: SequenceAnnotation
persistent identity: annotation1976850
display id: annotation1976850
version: 1
top level: LuxPQ
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K131015/annotation1976850/range1976850/1

https://synbiohub.org/public/igem/BBa_K131015/annotation1976850/range1976850/1
type: Range
persistent identity: range1976850
display id: range1976850
version: 1
direction: direction/forward
top level: LuxPQ
start: 30
end: 1127
orientation: inline

Start of LuxQ
type: SequenceAnnotation
persistent identity: annotation1976846
display id: annotation1976846
version: 1
top level: LuxPQ
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K131015/annotation1976846/range1976846/1

https://synbiohub.org/public/igem/BBa_K131015/annotation1976846/range1976846/1
type: Range
persistent identity: range1976846
display id: range1976846
version: 1
direction: direction/forward
top level: LuxPQ
start: 1127
end: 1129
orientation: inline

BBa_I13265
type: ComponentDefinition
persistent identity: BBa I13265
display id: BBa_I13265
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Lux based Sender/Receiver/Reporter system (LVA-)
creator: Barry Canton
created: 2004-08-08T11:00:00Z
modified: 2015-08-31T04:07:33Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_6_
m user id: 0
owner id: 135
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Analogue of the V. Fischeri quorum-sensing system. Built using Biobrick components. Includes LuxR and LuxI under the control of their natural promoters and includes a YFP output device on the same transcript as LuxI (Same as I13264 but LuxI has no LVA tag)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Signalling
role: SO:0000804
component: BBa_B0010
component: BBa_E0030
component: BBa_B0012
component: BBa_C0062
component: BBa_B0034
component: BBa_R0063
component: BBa_C0161
component: BBa_R0062
sequence annotation: BBa_B0034
sequence annotation: BBa_B0012
sequence annotation: BBa_B0010
sequence annotation: BBa_R0063
sequence annotation: BBa_C0161
sequence annotation: BBa_R0062
sequence annotation: BBa_E0030
sequence annotation: BBa_C0062
sequence: https://synbiohub.org/public/igem/BBa_I13265_sequence/1

BBa_B0010
type: Component
persistent identity: component1039890
display id: component1039890
version: 1
top level: BBa_I13265
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_E0030
type: Component
persistent identity: component1039932
display id: component1039932
version: 1
top level: BBa_I13265
access: public
definition: https://synbiohub.org/public/igem/BBa_E0030/1

BBa_B0012
type: Component
persistent identity: component1039947
display id: component1039947
version: 1
top level: BBa_I13265
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_C0062
type: Component
persistent identity: component1039882
display id: component1039882
version: 1
top level: BBa_I13265
access: public
definition: luxr

BBa_B0034
type: Component
persistent identity: component1039930
display id: component1039930
version: 1
top level: BBa_I13265
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_R0063
type: Component
persistent identity: component1039858
display id: component1039858
version: 1
top level: BBa_I13265
access: public
definition: https://synbiohub.org/public/igem/BBa_R0063/1

BBa_B0034
type: Component
persistent identity: component1039923
display id: component1039923
version: 1
top level: BBa_I13265
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0010
type: Component
persistent identity: component1039937
display id: component1039937
version: 1
top level: BBa_I13265
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_C0161
type: Component
persistent identity: component1039925
display id: component1039925
version: 1
top level: BBa_I13265
access: public
definition: luxI

BBa_B0012
type: Component
persistent identity: component1039900
display id: component1039900
version: 1
top level: BBa_I13265
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_R0062
type: Component
persistent identity: component1039915
display id: component1039915
version: 1
top level: BBa_I13265
access: public
definition: https://synbiohub.org/public/igem/BBa_R0062/1

BBa_B0034
type: Component
persistent identity: component1039867
display id: component1039867
version: 1
top level: BBa_I13265
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1039930
display id: annotation1039930
version: 1
top level: BBa_I13265
location: https://synbiohub.org/public/igem/BBa_I13265/annotation1039930/range1039930/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I13265/annotation1039930/range1039930/1
type: Range
persistent identity: range1039930
display id: range1039930
version: 1
direction: direction/forward
top level: BBa_I13265
start: 1778
end: 1789
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1039947
display id: annotation1039947
version: 1
top level: BBa_I13265
location: https://synbiohub.org/public/igem/BBa_I13265/annotation1039947/range1039947/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I13265/annotation1039947/range1039947/1
type: Range
persistent identity: range1039947
display id: range1039947
version: 1
direction: direction/forward
top level: BBa_I13265
start: 2615
end: 2655
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1039937
display id: annotation1039937
version: 1
top level: BBa_I13265
location: https://synbiohub.org/public/igem/BBa_I13265/annotation1039937/range1039937/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I13265/annotation1039937/range1039937/1
type: Range
persistent identity: range1039937
display id: range1039937
version: 1
direction: direction/forward
top level: BBa_I13265
start: 2527
end: 2606
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1039890
display id: annotation1039890
version: 1
top level: BBa_I13265
location: https://synbiohub.org/public/igem/BBa_I13265/annotation1039890/range1039890/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I13265/annotation1039890/range1039890/1
type: Range
persistent identity: range1039890
display id: range1039890
version: 1
direction: direction/forward
top level: BBa_I13265
start: 967
end: 1046
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1039867
display id: annotation1039867
version: 1
top level: BBa_I13265
location: https://synbiohub.org/public/igem/BBa_I13265/annotation1039867/range1039867/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I13265/annotation1039867/range1039867/1
type: Range
persistent identity: range1039867
display id: range1039867
version: 1
direction: direction/forward
top level: BBa_I13265
start: 160
end: 171
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1039923
display id: annotation1039923
version: 1
top level: BBa_I13265
location: https://synbiohub.org/public/igem/BBa_I13265/annotation1039923/range1039923/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I13265/annotation1039923/range1039923/1
type: Range
persistent identity: range1039923
display id: range1039923
version: 1
direction: direction/forward
top level: BBa_I13265
start: 1167
end: 1178
orientation: inline

BBa_R0063
type: SequenceAnnotation
persistent identity: annotation1039858
display id: annotation1039858
version: 1
top level: BBa_I13265
location: https://synbiohub.org/public/igem/BBa_I13265/annotation1039858/range1039858/1
component: BBa_R0063

https://synbiohub.org/public/igem/BBa_I13265/annotation1039858/range1039858/1
type: Range
persistent identity: range1039858
display id: range1039858
version: 1
direction: direction/forward
top level: BBa_I13265
start: 1
end: 151
orientation: inline

BBa_C0161
type: SequenceAnnotation
persistent identity: annotation1039925
display id: annotation1039925
version: 1
top level: BBa_I13265
location: https://synbiohub.org/public/igem/BBa_I13265/annotation1039925/range1039925/1
component: BBa_C0161

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type: Range
persistent identity: range1039925
display id: range1039925
version: 1
direction: direction/forward
top level: BBa_I13265
start: 1185
end: 1769
orientation: inline

BBa_R0062
type: SequenceAnnotation
persistent identity: annotation1039915
display id: annotation1039915
version: 1
top level: BBa_I13265
location: https://synbiohub.org/public/igem/BBa_I13265/annotation1039915/range1039915/1
component: BBa_R0062

https://synbiohub.org/public/igem/BBa_I13265/annotation1039915/range1039915/1
type: Range
persistent identity: range1039915
display id: range1039915
version: 1
direction: direction/forward
top level: BBa_I13265
start: 1104
end: 1158
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1039900
display id: annotation1039900
version: 1
top level: BBa_I13265
location: https://synbiohub.org/public/igem/BBa_I13265/annotation1039900/range1039900/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I13265/annotation1039900/range1039900/1
type: Range
persistent identity: range1039900
display id: range1039900
version: 1
direction: direction/forward
top level: BBa_I13265
start: 1055
end: 1095
orientation: inline

BBa_E0030
type: SequenceAnnotation
persistent identity: annotation1039932
display id: annotation1039932
version: 1
top level: BBa_I13265
location: https://synbiohub.org/public/igem/BBa_I13265/annotation1039932/range1039932/1
component: BBa_E0030

https://synbiohub.org/public/igem/BBa_I13265/annotation1039932/range1039932/1
type: Range
persistent identity: range1039932
display id: range1039932
version: 1
direction: direction/forward
top level: BBa_I13265
start: 1796
end: 2518
orientation: inline

BBa_C0062
type: SequenceAnnotation
persistent identity: annotation1039882
display id: annotation1039882
version: 1
top level: BBa_I13265
location: https://synbiohub.org/public/igem/BBa_I13265/annotation1039882/range1039882/1
component: BBa_C0062

https://synbiohub.org/public/igem/BBa_I13265/annotation1039882/range1039882/1
type: Range
persistent identity: range1039882
display id: range1039882
version: 1
direction: direction/forward
top level: BBa_I13265
start: 178
end: 933
orientation: inline

BBa_K1520024
type: ComponentDefinition
persistent identity: BBa K1520024
display id: BBa_K1520024
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Pcons2-rbs-lacI-Ter-Plac-rbs-luxI-Ter-Pcons2-rbs-luxR-Ter-Prlux-rbs-rfp-rbs-luxI-rbs-luxR-Ter
creator: yue wu
created: 2014-09-21T11:00:00Z
modified: 2015-05-08T01:10:49Z
discontinued: false
dominant: false
group u list: _1902_
m user id: 0
owner id: 21318
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Plasmid in positive feedback verification: Pcons2-rbs-lacI-Ter-Plac-rbs-luxI-Ter-Pcons2-rbs-luxR-Ter-Prlux-rbs-rfp-rbs-luxI-rbs-luxR-Ter It is a positive feedback plasmid using luxI and luxR as key to feedback. Introducing IPTG will produce rfp, luxI and luxR, and luxI together with luxR will work back to enhance production of rfp.
mutable notes: None
mutable provenance: Pcons2, lacI, Plac, luxI, luxR, Prlux, rfp, Ter
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1520020
component: BBa_K1520013
sequence annotation: BBa_K1520020
sequence annotation: BBa_K1520013
sequence: https://synbiohub.org/public/igem/BBa_K1520024_sequence/1

BBa_K1520020
type: Component
persistent identity: component2385250
display id: component2385250
version: 1
top level: BBa_K1520024
access: public
definition: BBa_K1520020

BBa_K1520013
type: Component
persistent identity: component2385264
display id: component2385264
version: 1
top level: BBa_K1520024
access: public
definition: BBa_K1520013

BBa_K1520020
type: SequenceAnnotation
persistent identity: annotation2385250
display id: annotation2385250
version: 1
top level: BBa_K1520024
location: https://synbiohub.org/public/igem/BBa_K1520024/annotation2385250/range2385250/1
component: BBa_K1520020

https://synbiohub.org/public/igem/BBa_K1520024/annotation2385250/range2385250/1
type: Range
persistent identity: range2385250
display id: range2385250
version: 1
direction: direction/reverse
top level: BBa_K1520024
start: 1
end: 3352
orientation: inline

BBa_K1520013
type: SequenceAnnotation
persistent identity: annotation2385264
display id: annotation2385264
version: 1
top level: BBa_K1520024
location: https://synbiohub.org/public/igem/BBa_K1520024/annotation2385264/range2385264/1
component: BBa_K1520013

https://synbiohub.org/public/igem/BBa_K1520024/annotation2385264/range2385264/1
type: Range
persistent identity: range2385264
display id: range2385264
version: 1
direction: direction/reverse
top level: BBa_K1520024
start: 3361
end: 5768
orientation: inline

BBa_I751250
type: ComponentDefinition
persistent identity: BBa I751250
display id: BBa_I751250
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pSB4 plac-luxI
creator: Kenichiro Iwasaki
created: 2007-10-22T11:00:00Z
modified: 2015-08-31T04:08:06Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _151_
m user id: 0
owner id: 2188
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0010
component: BBa_B0034
component: BBa_R0011
component: BBa_C0161
component: BBa_B0012
sequence annotation: BBa_B0012
sequence annotation: BBa_R0011
sequence annotation: BBa_B0010
sequence annotation: BBa_C0161
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_I751250_sequence/1

BBa_B0010
type: Component
persistent identity: component1953064
display id: component1953064
version: 1
top level: BBa_I751250
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0034
type: Component
persistent identity: component1953061
display id: component1953061
version: 1
top level: BBa_I751250
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_R0011
type: Component
persistent identity: component1953055
display id: component1953055
version: 1
top level: BBa_I751250
access: public
definition: https://synbiohub.org/public/igem/BBa_R0011/1

BBa_C0161
type: Component
persistent identity: component1953063
display id: component1953063
version: 1
top level: BBa_I751250
access: public
definition: luxI

BBa_B0012
type: Component
persistent identity: component1953066
display id: component1953066
version: 1
top level: BBa_I751250
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1953066
display id: annotation1953066
version: 1
top level: BBa_I751250
location: https://synbiohub.org/public/igem/BBa_I751250/annotation1953066/range1953066/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I751250/annotation1953066/range1953066/1
type: Range
persistent identity: range1953066
display id: range1953066
version: 1
direction: direction/forward
top level: BBa_I751250
start: 763
end: 803
orientation: inline

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation1953055
display id: annotation1953055
version: 1
top level: BBa_I751250
location: https://synbiohub.org/public/igem/BBa_I751250/annotation1953055/range1953055/1
component: BBa_R0011

https://synbiohub.org/public/igem/BBa_I751250/annotation1953055/range1953055/1
type: Range
persistent identity: range1953055
display id: range1953055
version: 1
direction: direction/forward
top level: BBa_I751250
start: 1
end: 54
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1953064
display id: annotation1953064
version: 1
top level: BBa_I751250
location: https://synbiohub.org/public/igem/BBa_I751250/annotation1953064/range1953064/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I751250/annotation1953064/range1953064/1
type: Range
persistent identity: range1953064
display id: range1953064
version: 1
direction: direction/forward
top level: BBa_I751250
start: 675
end: 754
orientation: inline

BBa_C0161
type: SequenceAnnotation
persistent identity: annotation1953063
display id: annotation1953063
version: 1
top level: BBa_I751250
location: https://synbiohub.org/public/igem/BBa_I751250/annotation1953063/range1953063/1
component: BBa_C0161

https://synbiohub.org/public/igem/BBa_I751250/annotation1953063/range1953063/1
type: Range
persistent identity: range1953063
display id: range1953063
version: 1
direction: direction/reverse
top level: BBa_I751250
start: 82
end: 666
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1953061
display id: annotation1953061
version: 1
top level: BBa_I751250
location: https://synbiohub.org/public/igem/BBa_I751250/annotation1953061/range1953061/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I751250/annotation1953061/range1953061/1
type: Range
persistent identity: range1953061
display id: range1953061
version: 1
direction: direction/reverse
top level: BBa_I751250
start: 64
end: 75
orientation: inline

BBa_K1520019
type: ComponentDefinition
persistent identity: BBa K1520019
display id: BBa_K1520019
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Pcons2-rbs-lacI-Ter-Plac-rbs-luxI-Ter
creator: yue wu
created: 2014-09-21T11:00:00Z
modified: 2015-05-08T01:10:49Z
discontinued: false
dominant: false
group u list: _1902_
m user id: 0
owner id: 21318
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Pcons2-rbs-lacI-Ter-Plac-rbs-luxI-Ter It produces luxI when there is enough IPTG.
mutable notes: None
mutable provenance: Pcons2, lacI, Plac, luxI, Ter
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1520009
component: BBa_K1520008
sequence annotation: BBa_K1520008
sequence annotation: BBa_K1520009
sequence: https://synbiohub.org/public/igem/BBa_K1520019_sequence/1

BBa_K1520009
type: Component
persistent identity: component2385091
display id: component2385091
version: 1
top level: BBa_K1520019
access: public
definition: BBa_K1520009

BBa_K1520008
type: Component
persistent identity: component2385079
display id: component2385079
version: 1
top level: BBa_K1520019
access: public
definition: https://synbiohub.org/public/igem/BBa_K1520008/1

BBa_K1520008
type: SequenceAnnotation
persistent identity: annotation2385079
display id: annotation2385079
version: 1
top level: BBa_K1520019
location: https://synbiohub.org/public/igem/BBa_K1520019/annotation2385079/range2385079/1
component: BBa_K1520008

https://synbiohub.org/public/igem/BBa_K1520019/annotation2385079/range2385079/1
type: Range
persistent identity: range2385079
display id: range2385079
version: 1
direction: direction/reverse
top level: BBa_K1520019
start: 1
end: 1351
orientation: inline

BBa_K1520009
type: SequenceAnnotation
persistent identity: annotation2385091
display id: annotation2385091
version: 1
top level: BBa_K1520019
location: https://synbiohub.org/public/igem/BBa_K1520019/annotation2385091/range2385091/1
component: BBa_K1520009

https://synbiohub.org/public/igem/BBa_K1520019/annotation2385091/range2385091/1
type: Range
persistent identity: range2385091
display id: range2385091
version: 1
direction: direction/reverse
top level: BBa_K1520019
start: 1360
end: 2365
orientation: inline

BBa_K081019
type: ComponentDefinition
persistent identity: BBa K081019
display id: BBa_K081019
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: luxR protein generator under Plambda regulation
creator: Lorenzo Pasotti, Paolo Magni
created: 2008-10-18T11:00:00Z
modified: 2015-05-08T01:08:35Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _227_
m user id: 0
owner id: 2583
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: This device produces luxR constitutively under the strong promoter Plambda, in absence of cI repressor. Plambda can be turned off by cI repressor and luxR production cannot continue. Strong RBS (efficiency=0.6). Artificial 39 bp terminator (efficiency=0.99).
mutable notes: We used BioBrick Standard Assembly.
mutable provenance: Plambda: RBS: luxR: T:
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0030
component: BBa_R0051
component: BBa_B1006
component: BBa_C0062
sequence annotation: BBa_B1006
sequence annotation: BBa_C0062
sequence annotation: BBa_B0030
sequence annotation: BBa_R0051
sequence: https://synbiohub.org/public/igem/BBa_K081019_sequence/1

BBa_B0030
type: Component
persistent identity: component2249293
display id: component2249293
version: 1
top level: BBa_K081019
access: public
definition: https://synbiohub.org/public/igem/BBa_B0030/1

BBa_R0051
type: Component
persistent identity: component2249288
display id: component2249288
version: 1
top level: BBa_K081019
access: public
definition: https://synbiohub.org/public/igem/BBa_R0051/1

BBa_B1006
type: Component
persistent identity: component2249305
display id: component2249305
version: 1
top level: BBa_K081019
access: public
definition: https://synbiohub.org/public/igem/BBa_B1006/1

BBa_C0062
type: Component
persistent identity: component2249297
display id: component2249297
version: 1
top level: BBa_K081019
access: public
definition: luxr

BBa_B1006
type: SequenceAnnotation
persistent identity: annotation2249305
display id: annotation2249305
version: 1
top level: BBa_K081019
location: https://synbiohub.org/public/igem/BBa_K081019/annotation2249305/range2249305/1
component: BBa_B1006

https://synbiohub.org/public/igem/BBa_K081019/annotation2249305/range2249305/1
type: Range
persistent identity: range2249305
display id: range2249305
version: 1
direction: direction/reverse
top level: BBa_K081019
start: 868
end: 906
orientation: inline

BBa_C0062
type: SequenceAnnotation
persistent identity: annotation2249297
display id: annotation2249297
version: 1
top level: BBa_K081019
location: https://synbiohub.org/public/igem/BBa_K081019/annotation2249297/range2249297/1
component: BBa_C0062

https://synbiohub.org/public/igem/BBa_K081019/annotation2249297/range2249297/1
type: Range
persistent identity: range2249297
display id: range2249297
version: 1
direction: direction/forward
top level: BBa_K081019
start: 79
end: 834
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation2249293
display id: annotation2249293
version: 1
top level: BBa_K081019
location: https://synbiohub.org/public/igem/BBa_K081019/annotation2249293/range2249293/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_K081019/annotation2249293/range2249293/1
type: Range
persistent identity: range2249293
display id: range2249293
version: 1
direction: direction/forward
top level: BBa_K081019
start: 58
end: 72
orientation: inline

BBa_R0051
type: SequenceAnnotation
persistent identity: annotation2249288
display id: annotation2249288
version: 1
top level: BBa_K081019
location: https://synbiohub.org/public/igem/BBa_K081019/annotation2249288/range2249288/1
component: BBa_R0051

https://synbiohub.org/public/igem/BBa_K081019/annotation2249288/range2249288/1
type: Range
persistent identity: range2249288
display id: range2249288
version: 1
direction: direction/forward
top level: BBa_K081019
start: 1
end: 49
orientation: inline

BBa_K1631003
type: ComponentDefinition
persistent identity: BBa K1631003
display id: BBa_K1631003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Tlanslational unit of Colicin Lysis Protein (for colicin-E3)
creator: Yuto Yamanaka
created: 2015-09-12T11:00:00Z
modified: 2015-09-18T06:28:24Z
discontinued: false
dominant: false
group u list: _2048_
m user id: 23567
owner id: 23567
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Colicin lysis protein allows colicins to be released. The mechanism how the colicin lysis protein allows colicin release has not been fully elucidated, but it is sure that this protein raise membrane permiability and cause quasilysis.[1] Colicins are a cytotoxins which are released to environment and kill other related strains.
mutable notes: none
mutable provenance: pColE3-CA38
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
sequence annotation: BBa_B0034
sequence annotation: Colicin Lysis Protein
sequence: https://synbiohub.org/public/igem/BBa_K1631003_sequence/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2453045
display id: annotation2453045
version: 1
top level: BBa_K1631003
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1631003/annotation2453045/range2453045/1

https://synbiohub.org/public/igem/BBa_K1631003/annotation2453045/range2453045/1
type: Range
persistent identity: range2453045
display id: range2453045
version: 1
direction: direction/forward
top level: BBa_K1631003
start: 1
end: 12
orientation: inline

Colicin Lysis Protein
type: SequenceAnnotation
persistent identity: annotation2453046
display id: annotation2453046
version: 1
top level: BBa_K1631003
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1631003/annotation2453046/range2453046/1

https://synbiohub.org/public/igem/BBa_K1631003/annotation2453046/range2453046/1
type: Range
persistent identity: range2453046
display id: range2453046
version: 1
direction: direction/forward
top level: BBa_K1631003
start: 13
end: 156
orientation: inline

BBa_K1631002
type: ComponentDefinition
persistent identity: BBa K1631002
display id: BBa_K1631002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Translational unit of Colicin-E3
creator: Yuto Yamanaka
created: 2015-09-11T11:00:00Z
modified: 2015-09-14T03:43:50Z
discontinued: false
dominant: false
group u list: _2048_
m user id: 23567
owner id: 23567
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Colicin-E3 cleave 16SrRNA at a specific site. This activity is inhibited by immunity protein for Colicin-E3(BBa_). When lysis protein for Colicin-E3(BBa_) is co-expressed, Colicin-E3 is emited to environment and kill other strain. Colicin is a type of bacteriocin produced by Escherichia coli.
mutable notes: none
mutable provenance: pColE3-CA38
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
sequence annotation: BBa_B0034
sequence annotation: Colicin-E3
sequence: https://synbiohub.org/public/igem/BBa_K1631002_sequence/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2452986
display id: annotation2452986
version: 1
top level: BBa_K1631002
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1631002/annotation2452986/range2452986/1

https://synbiohub.org/public/igem/BBa_K1631002/annotation2452986/range2452986/1
type: Range
persistent identity: range2452986
display id: range2452986
version: 1
direction: direction/forward
top level: BBa_K1631002
start: 1
end: 12
orientation: inline

Colicin-E3
type: SequenceAnnotation
persistent identity: annotation2452987
display id: annotation2452987
version: 1
top level: BBa_K1631002
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1631002/annotation2452987/range2452987/1

https://synbiohub.org/public/igem/BBa_K1631002/annotation2452987/range2452987/1
type: Range
persistent identity: range2452987
display id: range2452987
version: 1
direction: direction/forward
top level: BBa_K1631002
start: 13
end: 1668
orientation: inline

BBa_K772002
type: ComponentDefinition
persistent identity: BBa K772002
display id: BBa_K772002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: EpCAM Binding Dock: N terminus
creator: Mustafa Elitok
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:15Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1024_
m user id: 0
owner id: 9573
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: This gene sequence includes an EpCAM (Epithelial p? Cell Adhesion Molecule) antigen binding domain, OmpA transmembrane protein (dock) and TEV protease N terminus. It is planned to use this subpart with BBa_772, which contains only TEV protease C terminus differently instead of N terminus, in order to induce a signal reaction in the case of EpCAM presence. EpCAM is an natural antigen which is found high significantly in the cases of some breast and colorectal cancer subtypes. On the upstream region of protein sequence, there are a constitutive pLac promoter and appropiate RBS. To see how this part works: Experience.
mutable notes: There are two protein sequences between domains and the dock: One between the binding domain (antibody) and dock and one between the part of TEV protease.
mutable provenance: CD225 (EpCAM antibody) is commonly used for the test of presence of EpCAM in the laboratories. The sequences of OmpA and TEV protease terminuses are acquired from the Registry.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
sequence annotation: CD215 antibody
sequence annotation: OmpA (Dock)
sequence annotation: pCons Promoter
sequence annotation: RBS
sequence annotation: Binding Dock
sequence annotation: TEV Protease N terminus
sequence: https://synbiohub.org/public/igem/BBa_K772002_sequence/1

CD215 antibody
type: SequenceAnnotation
persistent identity: annotation2203686
display id: annotation2203686
version: 1
top level: BBa_K772002
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K772002/annotation2203686/range2203686/1

https://synbiohub.org/public/igem/BBa_K772002/annotation2203686/range2203686/1
type: Range
persistent identity: range2203686
display id: range2203686
version: 1
direction: direction/forward
top level: BBa_K772002
start: 837
end: 1138
orientation: inline

OmpA (Dock)
type: SequenceAnnotation
persistent identity: annotation2203682
display id: annotation2203682
version: 1
top level: BBa_K772002
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K772002/annotation2203682/range2203682/1

https://synbiohub.org/public/igem/BBa_K772002/annotation2203682/range2203682/1
type: Range
persistent identity: range2203682
display id: range2203682
version: 1
direction: direction/forward
top level: BBa_K772002
start: 399
end: 836
orientation: inline

pCons Promoter
type: SequenceAnnotation
persistent identity: annotation2203681
display id: annotation2203681
version: 1
top level: BBa_K772002
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K772002/annotation2203681/range2203681/1

https://synbiohub.org/public/igem/BBa_K772002/annotation2203681/range2203681/1
type: Range
persistent identity: range2203681
display id: range2203681
version: 1
direction: direction/forward
top level: BBa_K772002
start: 1
end: 72
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2203683
display id: annotation2203683
version: 1
top level: BBa_K772002
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K772002/annotation2203683/range2203683/1

https://synbiohub.org/public/igem/BBa_K772002/annotation2203683/range2203683/1
type: Range
persistent identity: range2203683
display id: range2203683
version: 1
direction: direction/forward
top level: BBa_K772002
start: 73
end: 93
orientation: inline

Binding Dock
type: SequenceAnnotation
persistent identity: annotation2203684
display id: annotation2203684
version: 1
top level: BBa_K772002
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K772002/annotation2203684/range2203684/1

https://synbiohub.org/public/igem/BBa_K772002/annotation2203684/range2203684/1
type: Range
persistent identity: range2203684
display id: range2203684
version: 1
direction: direction/forward
top level: BBa_K772002
start: 94
end: 1138
orientation: inline

TEV Protease N terminus
type: SequenceAnnotation
persistent identity: annotation2203685
display id: annotation2203685
version: 1
top level: BBa_K772002
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K772002/annotation2203685/range2203685/1

https://synbiohub.org/public/igem/BBa_K772002/annotation2203685/range2203685/1
type: Range
persistent identity: range2203685
display id: range2203685
version: 1
direction: direction/forward
top level: BBa_K772002
start: 94
end: 398
orientation: inline

aiiA
type: ComponentDefinition
persistent identity: BBa C0060
display id: BBa_C0060
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: autoinducer inactivation enzyme from Bacillus; hydrolyzes acetyl homoserine lactone
creator: Vinay S Mahajan, Brian Chow, Peter Carr, Voichita D. Marinescu and Alexander D. Wissner-Gross
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:23Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Coding region for the autoinducer inactivation enzyme A (aiiA) LVA tagged. The gene was originally isolated from Bacillus sp. 240B1 and it encodes an enzyme that catalyzes the degradation of N-acyl homoserine lactones (AHLs)--quorum sensing autoinducers.
mutable notes: References (unparsed) here: Dong,Y.H., Xu,J.L., Li,X.Z. and Zhang,L.H.: AiiA, an enzyme that inactivates the acylhomoserine lactone quorum-sensing signal and attenuates the virulence of Erwinia carotovora, Proc. Natl. Acad. Sci. U.S.A. 97 (7), 3526-3531 (2000). http://www.pnas.org/cgi/content/full/97/7/3526 Lee SJ, Park SY, Lee JJ, Yum DY, Koo BT, Lee JK.: Genes encoding the N-acyl homoserine lactone-degrading enzyme are widespread in many subspecies of Bacillus thuringiensis, Appl Environ Microbiol 2002 Aug;68(8):3919-24. http://aem.asm.org/cgi/content/full/68/8/3919?view=full&pmid=12147491 References (unparsed) here: Dong,Y.H., Xu,J.L., Li,X.Z. and Zhang,L.H.: AiiA, an enzyme that inactivates the acylhomoserine lactone quorum-sensing signal and attenuates the virulence of Erwinia carotovora, Proc. Natl. Acad. Sci. U.S.A. 97 (7), 3526-3531 (2000). http://www.pnas.org/cgi/content/full/97/7/3526 Lee SJ, Park SY, Lee JJ, Yum DY, Koo BT, Lee JK.: Genes encoding the N-acyl homoserine lactone-degrading enzyme are widespread in many subspecies of Bacillus thuringiensis, Appl Environ Microbiol 2002 Aug;68(8):3919-24. http://aem.asm.org/cgi/content/full/68/8/3919?view=full&pmid=12147491 BBa_C0060 insert contains open reading frame (nucleotides 49-801) of the GeneBank sequence AF196486 followed by the LVA tag and two double stop codons inserted in the BioBrick prefix and suffix flanking regions. The original stop codon was TAG and in the present sequence it was substituted by TAATAA.
mutable provenance: AF196486 from Bacillus sp. 240B1 putative metallohydrolase (aiiA) gene. Dong,Y.H., Xu,J.L., Li,X.Z. and Zhang,L.H.: AiiA, an enzyme that inactivates the acylhomoserine lactone quorum-sensing signal and attenuates the virulence of Erwinia carotovora, Proc. Natl. Acad. Sci. U.S.A. 97 (7), 3526-3531 (2000).
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: 2
sequence annotation: BBa_C0060
sequence annotation: Help:Barcodes
sequence annotation: aiiA
sequence annotation: start
sequence annotation: LVA
sequence: https://synbiohub.org/public/igem/BBa_C0060_sequence/1

2
type: SequenceAnnotation
persistent identity: annotation1755
display id: annotation1755
version: 1
top level: aiiA
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_C0060/annotation1755/range1755/1

https://synbiohub.org/public/igem/BBa_C0060/annotation1755/range1755/1
type: Range
persistent identity: range1755
display id: range1755
version: 1
direction: direction/forward
top level: aiiA
start: 784
end: 789
orientation: inline

BBa_C0060
type: SequenceAnnotation
persistent identity: annotation7037
display id: annotation7037
version: 1
top level: aiiA
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_C0060/annotation7037/range7037/1

https://synbiohub.org/public/igem/BBa_C0060/annotation7037/range7037/1
type: Range
persistent identity: range7037
display id: range7037
version: 1
direction: direction/forward
top level: aiiA
start: 1
end: 789
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213987
display id: annotation2213987
version: 1
top level: aiiA
role: SO:0000807
role: feature/barcode
location: https://synbiohub.org/public/igem/BBa_C0060/annotation2213987/range2213987/1

https://synbiohub.org/public/igem/BBa_C0060/annotation2213987/range2213987/1
type: Range
persistent identity: range2213987
display id: range2213987
version: 1
direction: direction/forward
top level: aiiA
start: 790
end: 814
orientation: inline

aiiA
type: SequenceAnnotation
persistent identity: annotation1757
display id: annotation1757
version: 1
top level: aiiA
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_C0060/annotation1757/range1757/1

https://synbiohub.org/public/igem/BBa_C0060/annotation1757/range1757/1
type: Range
persistent identity: range1757
display id: range1757
version: 1
direction: direction/forward
top level: aiiA
start: 1
end: 750
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation1754
display id: annotation1754
version: 1
top level: aiiA
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_C0060/annotation1754/range1754/1

https://synbiohub.org/public/igem/BBa_C0060/annotation1754/range1754/1
type: Range
persistent identity: range1754
display id: range1754
version: 1
direction: direction/forward
top level: aiiA
start: 1
end: 3
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation1756
display id: annotation1756
version: 1
top level: aiiA
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_C0060/annotation1756/range1756/1

https://synbiohub.org/public/igem/BBa_C0060/annotation1756/range1756/1
type: Range
persistent identity: range1756
display id: range1756
version: 1
direction: direction/forward
top level: aiiA
start: 751
end: 783
orientation: inline

BBa_F1610
type: ComponentDefinition
persistent identity: BBa F1610
display id: BBa_F1610
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: 3OC6HSL Sender Device
creator: Caitlin Conboy and Jennifer Braff
created: 2003-12-04T12:00:00Z
modified: 2015-08-31T04:07:27Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This device accepts PoPS as input and produces the LuxI enzyme. This enzyme produces 3OC6HSL.
mutable provenance: Formerly I0461
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Signalling
role: SO:0000804
component: BBa_C0061
component: BBa_B0012
component: BBa_B0010
component: BBa_B0034
sequence annotation: BBa_C0061
sequence annotation: BBa_B0034
sequence annotation: BBa_B0012
sequence annotation: BBa_B0010
sequence: https://synbiohub.org/public/igem/BBa_F1610_sequence/1

BBa_C0061
type: Component
persistent identity: component943143
display id: component943143
version: 1
top level: BBa_F1610
access: public
definition: luxI

BBa_B0012
type: Component
persistent identity: component943160
display id: component943160
version: 1
top level: BBa_F1610
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0010
type: Component
persistent identity: component943150
display id: component943150
version: 1
top level: BBa_F1610
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0034
type: Component
persistent identity: component943133
display id: component943133
version: 1
top level: BBa_F1610
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_C0061
type: SequenceAnnotation
persistent identity: annotation943143
display id: annotation943143
version: 1
top level: BBa_F1610
location: https://synbiohub.org/public/igem/BBa_F1610/annotation943143/range943143/1
component: BBa_C0061

https://synbiohub.org/public/igem/BBa_F1610/annotation943143/range943143/1
type: Range
persistent identity: range943143
display id: range943143
version: 1
direction: direction/forward
top level: BBa_F1610
start: 19
end: 636
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation943133
display id: annotation943133
version: 1
top level: BBa_F1610
location: https://synbiohub.org/public/igem/BBa_F1610/annotation943133/range943133/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_F1610/annotation943133/range943133/1
type: Range
persistent identity: range943133
display id: range943133
version: 1
direction: direction/forward
top level: BBa_F1610
start: 1
end: 12
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation943160
display id: annotation943160
version: 1
top level: BBa_F1610
location: https://synbiohub.org/public/igem/BBa_F1610/annotation943160/range943160/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_F1610/annotation943160/range943160/1
type: Range
persistent identity: range943160
display id: range943160
version: 1
direction: direction/forward
top level: BBa_F1610
start: 758
end: 798
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation943150
display id: annotation943150
version: 1
top level: BBa_F1610
location: https://synbiohub.org/public/igem/BBa_F1610/annotation943150/range943150/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_F1610/annotation943150/range943150/1
type: Range
persistent identity: range943150
display id: range943150
version: 1
direction: direction/forward
top level: BBa_F1610
start: 670
end: 749
orientation: inline

BBa_K094112
type: ComponentDefinition
persistent identity: BBa K094112
display id: BBa_K094112
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: luxR-luxI-terminator
creator: Shi Lei
created: 2008-10-13T11:00:00Z
modified: 2015-05-08T01:08:40Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _255_
m user id: 0
owner id: 2589
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: the cheY* gene is installed in front of mRFP operon. cheY is the gene coding for cheY protein which controls flagella movement. This sequence, however, is mutated to give a mutant cheY protein which will not respond to the presence of cheZ protein. This cheY* gene is without a promoter, rbs or terminator.
mutable notes: the cheY* gene is without an rbs nor a terminator
mutable provenance: composited from K094110 and J04450
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_K094013
component: BBa_C0061
component: BBa_B0034
component: BBa_C0062
sequence annotation: BBa_C0061
sequence annotation: BBa_B0034
sequence annotation: BBa_K094013
sequence annotation: BBa_C0062
sequence: https://synbiohub.org/public/igem/BBa_K094112_sequence/1

BBa_K094013
type: Component
persistent identity: component2249256
display id: component2249256
version: 1
top level: BBa_K094112
access: public
definition: https://synbiohub.org/public/igem/BBa_K094013/1

BBa_C0061
type: Component
persistent identity: component2249253
display id: component2249253
version: 1
top level: BBa_K094112
access: public
definition: luxI

BBa_B0034
type: Component
persistent identity: component2249251
display id: component2249251
version: 1
top level: BBa_K094112
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_C0062
type: Component
persistent identity: component2249246
display id: component2249246
version: 1
top level: BBa_K094112
access: public
definition: luxr

BBa_C0061
type: SequenceAnnotation
persistent identity: annotation2249253
display id: annotation2249253
version: 1
top level: BBa_K094112
location: https://synbiohub.org/public/igem/BBa_K094112/annotation2249253/range2249253/1
component: BBa_C0061

https://synbiohub.org/public/igem/BBa_K094112/annotation2249253/range2249253/1
type: Range
persistent identity: range2249253
display id: range2249253
version: 1
direction: direction/forward
top level: BBa_K094112
start: 808
end: 1425
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2249251
display id: annotation2249251
version: 1
top level: BBa_K094112
location: https://synbiohub.org/public/igem/BBa_K094112/annotation2249251/range2249251/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K094112/annotation2249251/range2249251/1
type: Range
persistent identity: range2249251
display id: range2249251
version: 1
direction: direction/reverse
top level: BBa_K094112
start: 790
end: 801
orientation: inline

BBa_K094013
type: SequenceAnnotation
persistent identity: annotation2249256
display id: annotation2249256
version: 1
top level: BBa_K094112
location: https://synbiohub.org/public/igem/BBa_K094112/annotation2249256/range2249256/1
component: BBa_K094013

https://synbiohub.org/public/igem/BBa_K094112/annotation2249256/range2249256/1
type: Range
persistent identity: range2249256
display id: range2249256
version: 1
direction: direction/reverse
top level: BBa_K094112
start: 1459
end: 1552
orientation: inline

BBa_C0062
type: SequenceAnnotation
persistent identity: annotation2249246
display id: annotation2249246
version: 1
top level: BBa_K094112
location: https://synbiohub.org/public/igem/BBa_K094112/annotation2249246/range2249246/1
component: BBa_C0062

https://synbiohub.org/public/igem/BBa_K094112/annotation2249246/range2249246/1
type: Range
persistent identity: range2249246
display id: range2249246
version: 1
direction: direction/forward
top level: BBa_K094112
start: 1
end: 756
orientation: inline

BBa_J06101
type: ComponentDefinition
persistent identity: BBa J06101
display id: BBa_J06101
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Tet repressible lasI generator ( LVA+ )
creator: kxjin
created: 2005-06-20T11:00:00Z
modified: 2015-08-31T04:08:16Z
discontinued: false
dominant: true
experience: experience/None
group u list: _20_
m user id: 0
owner id: 368
owning group id: 20
sample status: In stock
status: status/Available
bookmark: false
mutable description: Generates lasI in response to aTc input.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Signalling
role: SO:0000804
component: BBa_B0034
component: BBa_B0012
component: BBa_R0040
component: BBa_B0010
component: BBa_C0078
sequence annotation: BBa_B0012
sequence annotation: BBa_C0078
sequence annotation: BBa_B0034
sequence annotation: BBa_B0010
sequence annotation: BBa_R0040
sequence: https://synbiohub.org/public/igem/BBa_J06101_sequence/1

BBa_B0034
type: Component
persistent identity: component1528390
display id: component1528390
version: 1
top level: BBa_J06101
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0012
type: Component
persistent identity: component1528416
display id: component1528416
version: 1
top level: BBa_J06101
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_R0040
type: Component
persistent identity: component1528382
display id: component1528382
version: 1
top level: BBa_J06101
access: public
definition: https://synbiohub.org/public/igem/BBa_R0040/1

BBa_B0010
type: Component
persistent identity: component1528406
display id: component1528406
version: 1
top level: BBa_J06101
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_C0078
type: Component
persistent identity: component1528400
display id: component1528400
version: 1
top level: BBa_J06101
access: public
definition: lasI

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1528416
display id: annotation1528416
version: 1
top level: BBa_J06101
location: https://synbiohub.org/public/igem/BBa_J06101/annotation1528416/range1528416/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_J06101/annotation1528416/range1528416/1
type: Range
persistent identity: range1528416
display id: range1528416
version: 1
direction: direction/forward
top level: BBa_J06101
start: 844
end: 884
orientation: inline

BBa_C0078
type: SequenceAnnotation
persistent identity: annotation1528400
display id: annotation1528400
version: 1
top level: BBa_J06101
location: https://synbiohub.org/public/igem/BBa_J06101/annotation1528400/range1528400/1
component: BBa_C0078

https://synbiohub.org/public/igem/BBa_J06101/annotation1528400/range1528400/1
type: Range
persistent identity: range1528400
display id: range1528400
version: 1
direction: direction/forward
top level: BBa_J06101
start: 81
end: 722
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1528390
display id: annotation1528390
version: 1
top level: BBa_J06101
location: https://synbiohub.org/public/igem/BBa_J06101/annotation1528390/range1528390/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_J06101/annotation1528390/range1528390/1
type: Range
persistent identity: range1528390
display id: range1528390
version: 1
direction: direction/forward
top level: BBa_J06101
start: 63
end: 74
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1528406
display id: annotation1528406
version: 1
top level: BBa_J06101
location: https://synbiohub.org/public/igem/BBa_J06101/annotation1528406/range1528406/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_J06101/annotation1528406/range1528406/1
type: Range
persistent identity: range1528406
display id: range1528406
version: 1
direction: direction/forward
top level: BBa_J06101
start: 756
end: 835
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1528382
display id: annotation1528382
version: 1
top level: BBa_J06101
location: https://synbiohub.org/public/igem/BBa_J06101/annotation1528382/range1528382/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_J06101/annotation1528382/range1528382/1
type: Range
persistent identity: range1528382
display id: range1528382
version: 1
direction: direction/forward
top level: BBa_J06101
start: 1
end: 54
orientation: inline

BBa_K1072018
type: ComponentDefinition
persistent identity: BBa K1072018
display id: BBa_K1072018
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pGAL1 + Flag + Brho + odr-10 + GFP + ADH1Te + pFUS1 + BFP + ADH1Te
creator: Junqi kuang
created: 2013-09-21T11:00:00Z
modified: 2015-05-08T01:09:01Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1381_
m user id: 0
owner id: 16002
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: -
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1072020
component: BBa_K1072014
sequence annotation: BBa_K1072014
sequence annotation: BBa_K1072020
sequence: https://synbiohub.org/public/igem/BBa_K1072018_sequence/1

BBa_K1072020
type: Component
persistent identity: component2366679
display id: component2366679
version: 1
top level: BBa_K1072018
access: public
definition: https://synbiohub.org/public/igem/BBa_K1072020/1

BBa_K1072014
type: Component
persistent identity: component2366672
display id: component2366672
version: 1
top level: BBa_K1072018
access: public
definition: https://synbiohub.org/public/igem/BBa_K1072014/1

BBa_K1072014
type: SequenceAnnotation
persistent identity: annotation2366672
display id: annotation2366672
version: 1
top level: BBa_K1072018
location: https://synbiohub.org/public/igem/BBa_K1072018/annotation2366672/range2366672/1
component: BBa_K1072014

https://synbiohub.org/public/igem/BBa_K1072018/annotation2366672/range2366672/1
type: Range
persistent identity: range2366672
display id: range2366672
version: 1
direction: direction/reverse
top level: BBa_K1072018
start: 1
end: 2757
orientation: inline

BBa_K1072020
type: SequenceAnnotation
persistent identity: annotation2366679
display id: annotation2366679
version: 1
top level: BBa_K1072018
location: https://synbiohub.org/public/igem/BBa_K1072018/annotation2366679/range2366679/1
component: BBa_K1072020

https://synbiohub.org/public/igem/BBa_K1072018/annotation2366679/range2366679/1
type: Range
persistent identity: range2366679
display id: range2366679
version: 1
direction: direction/reverse
top level: BBa_K1072018
start: 2766
end: 4235
orientation: inline

BBa_I729005
type: ComponentDefinition
persistent identity: BBa I729005
display id: BBa_I729005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: AHL Reporter and Quencher
creator: Hiroki Fukutomi
created: 2007-10-22T11:00:00Z
modified: 2015-08-31T04:07:55Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _117_
m user id: 0
owner id: 1585
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: GFP and aiiA are repressed Lux promoter GFP and aiiA induced by AHL. GFP will express if the amount of AHL exeed the capability of aiiA.
mutable provenance: Biobrick -> ligation
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_F2620
component: BBa_C0260
component: BBa_E5500
sequence annotation: BBa_E5500
sequence annotation: BBa_C0260
sequence annotation: BBa_F2620
sequence: https://synbiohub.org/public/igem/BBa_I729005_sequence/1

BBa_F2620
type: Component
persistent identity: component2280694
display id: component2280694
version: 1
top level: BBa_I729005
access: public
definition: BBa_F2620

BBa_C0260
type: Component
persistent identity: component2280703
display id: component2280703
version: 1
top level: BBa_I729005
access: public
definition: BBa_C0260

BBa_E5500
type: Component
persistent identity: component2280708
display id: component2280708
version: 1
top level: BBa_I729005
access: public
definition: https://synbiohub.org/public/igem/BBa_E5500/1

BBa_E5500
type: SequenceAnnotation
persistent identity: annotation2280708
display id: annotation2280708
version: 1
top level: BBa_I729005
location: https://synbiohub.org/public/igem/BBa_I729005/annotation2280708/range2280708/1
component: BBa_E5500

https://synbiohub.org/public/igem/BBa_I729005/annotation2280708/range2280708/1
type: Range
persistent identity: range2280708
display id: range2280708
version: 1
direction: direction/reverse
top level: BBa_I729005
start: 1910
end: 2650
orientation: inline

BBa_C0260
type: SequenceAnnotation
persistent identity: annotation2280703
display id: annotation2280703
version: 1
top level: BBa_I729005
location: https://synbiohub.org/public/igem/BBa_I729005/annotation2280703/range2280703/1
component: BBa_C0260

https://synbiohub.org/public/igem/BBa_I729005/annotation2280703/range2280703/1
type: Range
persistent identity: range2280703
display id: range2280703
version: 1
direction: direction/reverse
top level: BBa_I729005
start: 1070
end: 1901
orientation: inline

BBa_F2620
type: SequenceAnnotation
persistent identity: annotation2280694
display id: annotation2280694
version: 1
top level: BBa_I729005
location: https://synbiohub.org/public/igem/BBa_I729005/annotation2280694/range2280694/1
component: BBa_F2620

https://synbiohub.org/public/igem/BBa_I729005/annotation2280694/range2280694/1
type: Range
persistent identity: range2280694
display id: range2280694
version: 1
direction: direction/reverse
top level: BBa_I729005
start: 1
end: 1061
orientation: inline

BBa_J06201
type: ComponentDefinition
persistent identity: BBa J06201
display id: BBa_J06201
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Tet repressible rhI generator (LVA+)
creator: sash31
created: 2005-06-23T11:00:00Z
modified: 2015-08-31T04:08:17Z
discontinued: false
dominant: true
experience: experience/None
group u list: _20_
m user id: 0
owner id: 339
owning group id: 20
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Generates rhI in response to aTc input.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Signalling
role: SO:0000804
component: BBa_B0012
component: BBa_C0070
component: BBa_B0010
component: BBa_B0034
component: BBa_R0040
sequence annotation: BBa_B0034
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_C0070
sequence annotation: BBa_R0040
sequence: https://synbiohub.org/public/igem/BBa_J06201_sequence/1

BBa_B0012
type: Component
persistent identity: component1538097
display id: component1538097
version: 1
top level: BBa_J06201
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_C0070
type: Component
persistent identity: component1538081
display id: component1538081
version: 1
top level: BBa_J06201
access: public
definition: rhII

BBa_B0010
type: Component
persistent identity: component1538087
display id: component1538087
version: 1
top level: BBa_J06201
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0034
type: Component
persistent identity: component1538071
display id: component1538071
version: 1
top level: BBa_J06201
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_R0040
type: Component
persistent identity: component1538063
display id: component1538063
version: 1
top level: BBa_J06201
access: public
definition: https://synbiohub.org/public/igem/BBa_R0040/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1538071
display id: annotation1538071
version: 1
top level: BBa_J06201
location: https://synbiohub.org/public/igem/BBa_J06201/annotation1538071/range1538071/1
component: BBa_B0034

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type: Range
persistent identity: range1538071
display id: range1538071
version: 1
direction: direction/forward
top level: BBa_J06201
start: 63
end: 74
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1538087
display id: annotation1538087
version: 1
top level: BBa_J06201
location: https://synbiohub.org/public/igem/BBa_J06201/annotation1538087/range1538087/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_J06201/annotation1538087/range1538087/1
type: Range
persistent identity: range1538087
display id: range1538087
version: 1
direction: direction/forward
top level: BBa_J06201
start: 756
end: 835
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1538097
display id: annotation1538097
version: 1
top level: BBa_J06201
location: https://synbiohub.org/public/igem/BBa_J06201/annotation1538097/range1538097/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_J06201/annotation1538097/range1538097/1
type: Range
persistent identity: range1538097
display id: range1538097
version: 1
direction: direction/forward
top level: BBa_J06201
start: 844
end: 884
orientation: inline

BBa_C0070
type: SequenceAnnotation
persistent identity: annotation1538081
display id: annotation1538081
version: 1
top level: BBa_J06201
location: https://synbiohub.org/public/igem/BBa_J06201/annotation1538081/range1538081/1
component: BBa_C0070

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type: Range
persistent identity: range1538081
display id: range1538081
version: 1
direction: direction/forward
top level: BBa_J06201
start: 81
end: 722
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1538063
display id: annotation1538063
version: 1
top level: BBa_J06201
location: https://synbiohub.org/public/igem/BBa_J06201/annotation1538063/range1538063/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_J06201/annotation1538063/range1538063/1
type: Range
persistent identity: range1538063
display id: range1538063
version: 1
direction: direction/forward
top level: BBa_J06201
start: 1
end: 54
orientation: inline

BBa_J06402
type: ComponentDefinition
persistent identity: BBa J06402
display id: BBa_J06402
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: C4HSL Receiver
creator: kxjin
created: 2005-06-22T11:00:00Z
modified: 2015-08-31T04:08:17Z
discontinued: false
dominant: false
experience: experience/None
group u list: _20_
m user id: 0
owner id: 368
owning group id: 20
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This device senses the C4HSL concentration in the media and produces output PoPS. RhlR production is controlled by constitutive P(BIa). This part is the C4HSL analog of F2621.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Signalling
role: SO:0000804
component: BBa_B0012
component: BBa_C0071
component: BBa_B0010
component: BBa_I14018
component: BBa_B0034
component: BBa_I14017
sequence annotation: BBa_B0012
sequence annotation: BBa_B0010
sequence annotation: BBa_C0071
sequence annotation: BBa_I14018
sequence annotation: BBa_I14017
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_J06402_sequence/1

BBa_B0012
type: Component
persistent identity: component1534549
display id: component1534549
version: 1
top level: BBa_J06402
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_C0071
type: Component
persistent identity: component1534533
display id: component1534533
version: 1
top level: BBa_J06402
access: public
definition: rhlR

BBa_B0010
type: Component
persistent identity: component1534539
display id: component1534539
version: 1
top level: BBa_J06402
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_I14018
type: Component
persistent identity: component1534518
display id: component1534518
version: 1
top level: BBa_J06402
access: public
definition: https://synbiohub.org/public/igem/BBa_I14018/1

BBa_B0034
type: Component
persistent identity: component1534523
display id: component1534523
version: 1
top level: BBa_J06402
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_I14017
type: Component
persistent identity: component1534563
display id: component1534563
version: 1
top level: BBa_J06402
access: public
definition: https://synbiohub.org/public/igem/BBa_I14017/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1534549
display id: annotation1534549
version: 1
top level: BBa_J06402
location: https://synbiohub.org/public/igem/BBa_J06402/annotation1534549/range1534549/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_J06402/annotation1534549/range1534549/1
type: Range
persistent identity: range1534549
display id: range1534549
version: 1
direction: direction/forward
top level: BBa_J06402
start: 945
end: 985
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1534539
display id: annotation1534539
version: 1
top level: BBa_J06402
location: https://synbiohub.org/public/igem/BBa_J06402/annotation1534539/range1534539/1
component: BBa_B0010

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type: Range
persistent identity: range1534539
display id: range1534539
version: 1
direction: direction/forward
top level: BBa_J06402
start: 857
end: 936
orientation: inline

BBa_C0071
type: SequenceAnnotation
persistent identity: annotation1534533
display id: annotation1534533
version: 1
top level: BBa_J06402
location: https://synbiohub.org/public/igem/BBa_J06402/annotation1534533/range1534533/1
component: BBa_C0071

https://synbiohub.org/public/igem/BBa_J06402/annotation1534533/range1534533/1
type: Range
persistent identity: range1534533
display id: range1534533
version: 1
direction: direction/forward
top level: BBa_J06402
start: 62
end: 823
orientation: inline

BBa_I14018
type: SequenceAnnotation
persistent identity: annotation1534518
display id: annotation1534518
version: 1
top level: BBa_J06402
location: https://synbiohub.org/public/igem/BBa_J06402/annotation1534518/range1534518/1
component: BBa_I14018

https://synbiohub.org/public/igem/BBa_J06402/annotation1534518/range1534518/1
type: Range
persistent identity: range1534518
display id: range1534518
version: 1
direction: direction/forward
top level: BBa_J06402
start: 1
end: 35
orientation: inline

BBa_I14017
type: SequenceAnnotation
persistent identity: annotation1534563
display id: annotation1534563
version: 1
top level: BBa_J06402
location: https://synbiohub.org/public/igem/BBa_J06402/annotation1534563/range1534563/1
component: BBa_I14017

https://synbiohub.org/public/igem/BBa_J06402/annotation1534563/range1534563/1
type: Range
persistent identity: range1534563
display id: range1534563
version: 1
direction: direction/forward
top level: BBa_J06402
start: 994
end: 1044
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1534523
display id: annotation1534523
version: 1
top level: BBa_J06402
location: https://synbiohub.org/public/igem/BBa_J06402/annotation1534523/range1534523/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_J06402/annotation1534523/range1534523/1
type: Range
persistent identity: range1534523
display id: range1534523
version: 1
direction: direction/forward
top level: BBa_J06402
start: 44
end: 55
orientation: inline

BBa_I9026
type: ComponentDefinition
persistent identity: BBa I9026
display id: BBa_I9026
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: rhlI translational unit
creator: crackdots
created: 2004-01-27T12:00:00Z
modified: 2015-08-31T04:08:11Z
discontinued: false
dominant: true
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_C0070
component: BBa_B0034
sequence annotation: BBa_C0070
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_I9026_sequence/1

BBa_C0070
type: Component
persistent identity: component939656
display id: component939656
version: 1
top level: BBa_I9026
access: public
definition: rhII

BBa_B0034
type: Component
persistent identity: component939646
display id: component939646
version: 1
top level: BBa_I9026
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_C0070
type: SequenceAnnotation
persistent identity: annotation939656
display id: annotation939656
version: 1
top level: BBa_I9026
location: https://synbiohub.org/public/igem/BBa_I9026/annotation939656/range939656/1
component: BBa_C0070

https://synbiohub.org/public/igem/BBa_I9026/annotation939656/range939656/1
type: Range
persistent identity: range939656
display id: range939656
version: 1
direction: direction/forward
top level: BBa_I9026
start: 19
end: 660
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation939646
display id: annotation939646
version: 1
top level: BBa_I9026
location: https://synbiohub.org/public/igem/BBa_I9026/annotation939646/range939646/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I9026/annotation939646/range939646/1
type: Range
persistent identity: range939646
display id: range939646
version: 1
direction: direction/forward
top level: BBa_I9026
start: 1
end: 12
orientation: inline

rhIR
type: ComponentDefinition
persistent identity: BBa C0171
display id: BBa_C0171
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: rhlR repressor/activator from P. aeruginosa PA3477 (no LVA)
creator: jcbraff
created: 2004-05-26T11:00:00Z
modified: 2015-08-31T04:07:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_1_
m user id: 0
owner id: 61
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: same as C0071 except no LVA tag
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_C0171_sequence/1

BBa_I13203
type: ComponentDefinition
persistent identity: BBa I13203
display id: BBa_I13203
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pBad.aiiA protein generator (LVA-)
creator: Barry Canton
created: 2004-07-29T11:00:00Z
modified: 2015-08-31T04:07:33Z
discontinued: false
dominant: true
experience: experience/None
group u list: _11_6_
m user id: 0
owner id: 135
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Outputs varying levels of aiiA as part of HSL/aiiA measurements. aiiA is untagged. Similar to I13204
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Signalling
role: SO:0000804
component: BBa_C0160
component: BBa_B0010
component: BBa_B0012
component: BBa_I0500
component: BBa_B0034
sequence annotation: BBa_B0034
sequence annotation: BBa_I0500
sequence annotation: BBa_B0012
sequence annotation: BBa_B0010
sequence annotation: BBa_C0160
sequence: https://synbiohub.org/public/igem/BBa_I13203_sequence/1

BBa_C0160
type: Component
persistent identity: component1251803
display id: component1251803
version: 1
top level: BBa_I13203
access: public
definition: aiiA

BBa_B0010
type: Component
persistent identity: component1251808
display id: component1251808
version: 1
top level: BBa_I13203
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
type: Component
persistent identity: component1251818
display id: component1251818
version: 1
top level: BBa_I13203
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_I0500
type: Component
persistent identity: component1251796
display id: component1251796
version: 1
top level: BBa_I13203
access: public
definition: https://synbiohub.org/public/igem/BBa_I0500/1

BBa_B0034
type: Component
persistent identity: component1251801
display id: component1251801
version: 1
top level: BBa_I13203
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1251801
display id: annotation1251801
version: 1
top level: BBa_I13203
location: https://synbiohub.org/public/igem/BBa_I13203/annotation1251801/range1251801/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I13203/annotation1251801/range1251801/1
type: Range
persistent identity: range1251801
display id: range1251801
version: 1
direction: direction/forward
top level: BBa_I13203
start: 1219
end: 1230
orientation: inline

BBa_I0500
type: SequenceAnnotation
persistent identity: annotation1251796
display id: annotation1251796
version: 1
top level: BBa_I13203
location: https://synbiohub.org/public/igem/BBa_I13203/annotation1251796/range1251796/1
component: BBa_I0500

https://synbiohub.org/public/igem/BBa_I13203/annotation1251796/range1251796/1
type: Range
persistent identity: range1251796
display id: range1251796
version: 1
direction: direction/forward
top level: BBa_I13203
start: 1
end: 1210
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1251818
display id: annotation1251818
version: 1
top level: BBa_I13203
location: https://synbiohub.org/public/igem/BBa_I13203/annotation1251818/range1251818/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I13203/annotation1251818/range1251818/1
type: Range
persistent identity: range1251818
display id: range1251818
version: 1
direction: direction/forward
top level: BBa_I13203
start: 2089
end: 2129
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1251808
display id: annotation1251808
version: 1
top level: BBa_I13203
location: https://synbiohub.org/public/igem/BBa_I13203/annotation1251808/range1251808/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I13203/annotation1251808/range1251808/1
type: Range
persistent identity: range1251808
display id: range1251808
version: 1
direction: direction/forward
top level: BBa_I13203
start: 2001
end: 2080
orientation: inline

BBa_C0160
type: SequenceAnnotation
persistent identity: annotation1251803
display id: annotation1251803
version: 1
top level: BBa_I13203
location: https://synbiohub.org/public/igem/BBa_I13203/annotation1251803/range1251803/1
component: BBa_C0160

https://synbiohub.org/public/igem/BBa_I13203/annotation1251803/range1251803/1
type: Range
persistent identity: range1251803
display id: range1251803
version: 1
direction: direction/forward
top level: BBa_I13203
start: 1237
end: 1992
orientation: inline

BBa_K1520013
type: ComponentDefinition
persistent identity: BBa K1520013
display id: BBa_K1520013
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Prlux-rbs-rfp-rbs-luxI-rbs-luxR-Ter
creator: yue wu
created: 2014-09-21T11:00:00Z
modified: 2015-05-08T01:10:49Z
discontinued: false
dominant: false
group u list: _1902_
m user id: 0
owner id: 21318
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Prlux-rbs-rfp-rbs-luxI-rbs-luxR-Ter. It produces rfp, luxI and luxR when there is enough luxR and AHL.
mutable notes: None
mutable provenance: Prlux, rfp, luxI, luxR, Ter
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1520029
component: BBa_K1520504
component: BBa_K1520500
component: BBa_K1520501
component: BBa_R0062
sequence annotation: BBa_K1520504
sequence annotation: BBa_K1520501
sequence annotation: BBa_K1520029
sequence annotation: BBa_R0062
sequence annotation: BBa_K1520500
sequence: https://synbiohub.org/public/igem/BBa_K1520013_sequence/1

BBa_K1520029
type: Component
persistent identity: component2384991
display id: component2384991
version: 1
top level: BBa_K1520013
access: public
definition: https://synbiohub.org/public/igem/BBa_K1520029/1

BBa_K1520504
type: Component
persistent identity: component2384987
display id: component2384987
version: 1
top level: BBa_K1520013
access: public
definition: BBa_K1520504

BBa_K1520500
type: Component
persistent identity: component2384984
display id: component2384984
version: 1
top level: BBa_K1520013
access: public
definition: https://synbiohub.org/public/igem/BBa_K1520500/1

BBa_K1520501
type: Component
persistent identity: component2384990
display id: component2384990
version: 1
top level: BBa_K1520013
access: public
definition: BBa_K1520501

BBa_R0062
type: Component
persistent identity: component2384979
display id: component2384979
version: 1
top level: BBa_K1520013
access: public
definition: https://synbiohub.org/public/igem/BBa_R0062/1

BBa_K1520504
type: SequenceAnnotation
persistent identity: annotation2384987
display id: annotation2384987
version: 1
top level: BBa_K1520013
location: https://synbiohub.org/public/igem/BBa_K1520013/annotation2384987/range2384987/1
component: BBa_K1520504

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type: Range
persistent identity: range2384987
display id: range2384987
version: 1
direction: direction/reverse
top level: BBa_K1520013
start: 804
end: 1464
orientation: inline

BBa_K1520501
type: SequenceAnnotation
persistent identity: annotation2384990
display id: annotation2384990
version: 1
top level: BBa_K1520013
location: https://synbiohub.org/public/igem/BBa_K1520013/annotation2384990/range2384990/1
component: BBa_K1520501

https://synbiohub.org/public/igem/BBa_K1520013/annotation2384990/range2384990/1
type: Range
persistent identity: range2384990
display id: range2384990
version: 1
direction: direction/reverse
top level: BBa_K1520013
start: 1473
end: 2271
orientation: inline

BBa_K1520029
type: SequenceAnnotation
persistent identity: annotation2384991
display id: annotation2384991
version: 1
top level: BBa_K1520013
location: https://synbiohub.org/public/igem/BBa_K1520013/annotation2384991/range2384991/1
component: BBa_K1520029

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type: Range
persistent identity: range2384991
display id: range2384991
version: 1
direction: direction/reverse
top level: BBa_K1520013
start: 2280
end: 2408
orientation: inline

BBa_R0062
type: SequenceAnnotation
persistent identity: annotation2384979
display id: annotation2384979
version: 1
top level: BBa_K1520013
location: https://synbiohub.org/public/igem/BBa_K1520013/annotation2384979/range2384979/1
component: BBa_R0062

https://synbiohub.org/public/igem/BBa_K1520013/annotation2384979/range2384979/1
type: Range
persistent identity: range2384979
display id: range2384979
version: 1
direction: direction/forward
top level: BBa_K1520013
start: 1
end: 55
orientation: inline

BBa_K1520500
type: SequenceAnnotation
persistent identity: annotation2384984
display id: annotation2384984
version: 1
top level: BBa_K1520013
location: https://synbiohub.org/public/igem/BBa_K1520013/annotation2384984/range2384984/1
component: BBa_K1520500

https://synbiohub.org/public/igem/BBa_K1520013/annotation2384984/range2384984/1
type: Range
persistent identity: range2384984
display id: range2384984
version: 1
direction: direction/reverse
top level: BBa_K1520013
start: 64
end: 795
orientation: inline

BBa_J06102
type: ComponentDefinition
persistent identity: BBa J06102
display id: BBa_J06102
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Tet repressible lasI generator ( LVA- )
creator: kxjin
created: 2005-06-20T11:00:00Z
modified: 2015-08-31T04:08:16Z
discontinued: false
dominant: false
experience: experience/None
group u list: _20_
m user id: 0
owner id: 368
owning group id: 20
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: LVA- version of J06101.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Signalling
role: SO:0000804
component: BBa_B0034
component: BBa_C0178
component: BBa_B0012
component: BBa_R0040
component: BBa_B0010
sequence annotation: BBa_B0034
sequence annotation: BBa_C0178
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_R0040
sequence: https://synbiohub.org/public/igem/BBa_J06102_sequence/1

BBa_B0034
type: Component
persistent identity: component1532380
display id: component1532380
version: 1
top level: BBa_J06102
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_C0178
type: Component
persistent identity: component1532382
display id: component1532382
version: 1
top level: BBa_J06102
access: public
definition: lasI

BBa_B0012
type: Component
persistent identity: component1532397
display id: component1532397
version: 1
top level: BBa_J06102
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_R0040
type: Component
persistent identity: component1532372
display id: component1532372
version: 1
top level: BBa_J06102
access: public
definition: https://synbiohub.org/public/igem/BBa_R0040/1

BBa_B0010
type: Component
persistent identity: component1532387
display id: component1532387
version: 1
top level: BBa_J06102
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1532380
display id: annotation1532380
version: 1
top level: BBa_J06102
location: https://synbiohub.org/public/igem/BBa_J06102/annotation1532380/range1532380/1
component: BBa_B0034

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type: Range
persistent identity: range1532380
display id: range1532380
version: 1
direction: direction/forward
top level: BBa_J06102
start: 63
end: 74
orientation: inline

BBa_C0178
type: SequenceAnnotation
persistent identity: annotation1532382
display id: annotation1532382
version: 1
top level: BBa_J06102
location: https://synbiohub.org/public/igem/BBa_J06102/annotation1532382/range1532382/1
component: BBa_C0178

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type: Range
persistent identity: range1532382
display id: range1532382
version: 1
direction: direction/forward
top level: BBa_J06102
start: 81
end: 689
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1532387
display id: annotation1532387
version: 1
top level: BBa_J06102
location: https://synbiohub.org/public/igem/BBa_J06102/annotation1532387/range1532387/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_J06102/annotation1532387/range1532387/1
type: Range
persistent identity: range1532387
display id: range1532387
version: 1
direction: direction/forward
top level: BBa_J06102
start: 698
end: 777
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1532397
display id: annotation1532397
version: 1
top level: BBa_J06102
location: https://synbiohub.org/public/igem/BBa_J06102/annotation1532397/range1532397/1
component: BBa_B0012

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type: Range
persistent identity: range1532397
display id: range1532397
version: 1
direction: direction/forward
top level: BBa_J06102
start: 786
end: 826
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1532372
display id: annotation1532372
version: 1
top level: BBa_J06102
location: https://synbiohub.org/public/igem/BBa_J06102/annotation1532372/range1532372/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_J06102/annotation1532372/range1532372/1
type: Range
persistent identity: range1532372
display id: range1532372
version: 1
direction: direction/forward
top level: BBa_J06102
start: 1
end: 54
orientation: inline

BBa_K772100
type: ComponentDefinition
persistent identity: BBa K772100
display id: BBa_K772100
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: EpCAM Detection Device
creator: Mustafa Elitok
created: 2012-09-25T11:00:00Z
modified: 2015-05-08T01:13:15Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1024_
m user id: 0
owner id: 9573
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This gene sequence includes both of EpCAM Binding Docks; BBa_K772001 and BBa_K772002. Thus, this composite part is the main component of CTC (Circulating Tumor Cell) detection system. Docks include an EpCAM (Epithelial Cell Adhesion Molecule) antigen binding domain, OmpA transmembrane protein (dock) and TEV protease C or N terminus. Hopefully, after binding to the EpCAM antigen, TEV protease domains will fix together to form the full structure in order to activate the signalling pathway. (For more information: BBa_K772003) EpCAM is an natural antigen which is found high significantly in the cases of some breast and colorectal cancer subtypes. On the upstream region of protein sequences, there are a constitutive pLac promoter and appropriate RBS. To see how this part works: Experience.
mutable notes: There are two protein sequences between domains and the dock: One between the binding domain (antibody) and dock and one between the domain of TEV protease.
mutable provenance: CD225 (EpCAM antibody) is commonly used for the test of presence of EpCAM in the laboratories. The sequences of OmpA and TEV protease terminuses are acquired from the Registry.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
component: BBa_K772001
component: BBa_K772002
sequence annotation: BBa_K772002
sequence annotation: BBa_K772001
sequence: https://synbiohub.org/public/igem/BBa_K772100_sequence/1

BBa_K772001
type: Component
persistent identity: component2211341
display id: component2211341
version: 1
top level: BBa_K772100
access: public
definition: BBa_K772001

BBa_K772002
type: Component
persistent identity: component2211348
display id: component2211348
version: 1
top level: BBa_K772100
access: public
definition: BBa_K772002

BBa_K772002
type: SequenceAnnotation
persistent identity: annotation2211348
display id: annotation2211348
version: 1
top level: BBa_K772100
location: https://synbiohub.org/public/igem/BBa_K772100/annotation2211348/range2211348/1
component: BBa_K772002

https://synbiohub.org/public/igem/BBa_K772100/annotation2211348/range2211348/1
type: Range
persistent identity: range2211348
display id: range2211348
version: 1
direction: direction/reverse
top level: BBa_K772100
start: 1168
end: 2305
orientation: inline

BBa_K772001
type: SequenceAnnotation
persistent identity: annotation2211341
display id: annotation2211341
version: 1
top level: BBa_K772100
location: https://synbiohub.org/public/igem/BBa_K772100/annotation2211341/range2211341/1
component: BBa_K772001

https://synbiohub.org/public/igem/BBa_K772100/annotation2211341/range2211341/1
type: Range
persistent identity: range2211341
display id: range2211341
version: 1
direction: direction/reverse
top level: BBa_K772100
start: 1
end: 1159
orientation: inline

BBa_K082030
type: ComponentDefinition
persistent identity: BBa K082030
display id: BBa_K082030
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: luxI generator on PSB3C5
creator: Yanting Xue , He Shen
created: 2008-10-28T12:00:00Z
modified: 2015-05-08T01:08:36Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _228_
m user id: 0
owner id: 1560
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: none
mutable notes: none
mutable provenance: none
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_R0040
component: BBa_C0261
component: BBa_B0015
sequence annotation: BBa_B0015
sequence annotation: BBa_C0261
sequence annotation: BBa_R0040
sequence: https://synbiohub.org/public/igem/BBa_K082030_sequence/1

BBa_R0040
type: Component
persistent identity: component2254800
display id: component2254800
version: 1
top level: BBa_K082030
access: public
definition: https://synbiohub.org/public/igem/BBa_R0040/1

BBa_C0261
type: Component
persistent identity: component2254811
display id: component2254811
version: 1
top level: BBa_K082030
access: public
definition: BBa_C0261

BBa_B0015
type: Component
persistent identity: component2254818
display id: component2254818
version: 1
top level: BBa_K082030
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2254818
display id: annotation2254818
version: 1
top level: BBa_K082030
location: https://synbiohub.org/public/igem/BBa_K082030/annotation2254818/range2254818/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K082030/annotation2254818/range2254818/1
type: Range
persistent identity: range2254818
display id: range2254818
version: 1
direction: direction/reverse
top level: BBa_K082030
start: 732
end: 860
orientation: inline

BBa_C0261
type: SequenceAnnotation
persistent identity: annotation2254811
display id: annotation2254811
version: 1
top level: BBa_K082030
location: https://synbiohub.org/public/igem/BBa_K082030/annotation2254811/range2254811/1
component: BBa_C0261

https://synbiohub.org/public/igem/BBa_K082030/annotation2254811/range2254811/1
type: Range
persistent identity: range2254811
display id: range2254811
version: 1
direction: direction/reverse
top level: BBa_K082030
start: 63
end: 723
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2254800
display id: annotation2254800
version: 1
top level: BBa_K082030
location: https://synbiohub.org/public/igem/BBa_K082030/annotation2254800/range2254800/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_K082030/annotation2254800/range2254800/1
type: Range
persistent identity: range2254800
display id: range2254800
version: 1
direction: direction/forward
top level: BBa_K082030
start: 1
end: 54
orientation: inline

BBa_K081020
type: ComponentDefinition
persistent identity: BBa K081020
display id: BBa_K081020
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: cI and lasR protein generator (TERM-)
creator: Lorenzo Pasotti, Paolo Magni
created: 2008-10-18T11:00:00Z
modified: 2015-05-08T01:08:35Z
discontinued: false
dominant: false
experience: experience/None
group u list: _227_
m user id: 0
owner id: 2583
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: This is a two-protein generator (cI and lasR) without transcriptional terminator. Strong RBS (efficiency=0.6).
mutable notes: We used BioBrick Standard Assembly.
mutable provenance: RBS: cI: lasR:
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_C0051
component: BBa_C0079
component: BBa_B0030
sequence annotation: BBa_B0030
sequence annotation: BBa_C0079
sequence annotation: BBa_C0051
sequence: https://synbiohub.org/public/igem/BBa_K081020_sequence/1

BBa_C0051
type: Component
persistent identity: component2249312
display id: component2249312
version: 1
top level: BBa_K081020
access: public
definition: https://synbiohub.org/public/igem/BBa_C0051/1

BBa_C0079
type: Component
persistent identity: component2249320
display id: component2249320
version: 1
top level: BBa_K081020
access: public
definition: lasr

BBa_B0030
type: Component
persistent identity: component2249314
display id: component2249314
version: 1
top level: BBa_K081020
access: public
definition: https://synbiohub.org/public/igem/BBa_B0030/1

BBa_B0030
type: Component
persistent identity: component2249307
display id: component2249307
version: 1
top level: BBa_K081020
access: public
definition: https://synbiohub.org/public/igem/BBa_B0030/1

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation2249314
display id: annotation2249314
version: 1
top level: BBa_K081020
location: https://synbiohub.org/public/igem/BBa_K081020/annotation2249314/range2249314/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_K081020/annotation2249314/range2249314/1
type: Range
persistent identity: range2249314
display id: range2249314
version: 1
direction: direction/forward
top level: BBa_K081020
start: 805
end: 819
orientation: inline

BBa_C0079
type: SequenceAnnotation
persistent identity: annotation2249320
display id: annotation2249320
version: 1
top level: BBa_K081020
location: https://synbiohub.org/public/igem/BBa_K081020/annotation2249320/range2249320/1
component: BBa_C0079

https://synbiohub.org/public/igem/BBa_K081020/annotation2249320/range2249320/1
type: Range
persistent identity: range2249320
display id: range2249320
version: 1
direction: direction/reverse
top level: BBa_K081020
start: 826
end: 1606
orientation: inline

BBa_C0051
type: SequenceAnnotation
persistent identity: annotation2249312
display id: annotation2249312
version: 1
top level: BBa_K081020
location: https://synbiohub.org/public/igem/BBa_K081020/annotation2249312/range2249312/1
component: BBa_C0051

https://synbiohub.org/public/igem/BBa_K081020/annotation2249312/range2249312/1
type: Range
persistent identity: range2249312
display id: range2249312
version: 1
direction: direction/reverse
top level: BBa_K081020
start: 22
end: 796
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation2249307
display id: annotation2249307
version: 1
top level: BBa_K081020
location: https://synbiohub.org/public/igem/BBa_K081020/annotation2249307/range2249307/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_K081020/annotation2249307/range2249307/1
type: Range
persistent identity: range2249307
display id: range2249307
version: 1
direction: direction/forward
top level: BBa_K081020
start: 1
end: 15
orientation: inline

BBa_K574006
type: ComponentDefinition
persistent identity: BBa K574006
display id: BBa_K574006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: 3OC12HSL regulated by pTet and pBad
creator: Lei Wei
created: 2011-09-28T11:00:00Z
modified: 2015-05-08T01:12:44Z
discontinued: false
dominant: false
experience: experience/None
group u list: _745_
m user id: 0
owner id: 8529
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: Part 2/3 of a Oscillator.
mutable notes: Empty.
mutable provenance: Existing Parts.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_K574004
component: BBa_K574005
sequence annotation: BBa_K574005
sequence annotation: BBa_K574004
sequence: https://synbiohub.org/public/igem/BBa_K574006_sequence/1

BBa_K574004
type: Component
persistent identity: component2295487
display id: component2295487
version: 1
top level: BBa_K574006
access: public
definition: BBa_K574004

BBa_K574005
type: Component
persistent identity: component2295507
display id: component2295507
version: 1
top level: BBa_K574006
access: public
definition: BBa_K574005

BBa_K574005
type: SequenceAnnotation
persistent identity: annotation2295507
display id: annotation2295507
version: 1
top level: BBa_K574006
location: https://synbiohub.org/public/igem/BBa_K574006/annotation2295507/range2295507/1
component: BBa_K574005

https://synbiohub.org/public/igem/BBa_K574006/annotation2295507/range2295507/1
type: Range
persistent identity: range2295507
display id: range2295507
version: 1
direction: direction/reverse
top level: BBa_K574006
start: 806
end: 2517
orientation: inline

BBa_K574004
type: SequenceAnnotation
persistent identity: annotation2295487
display id: annotation2295487
version: 1
top level: BBa_K574006
location: https://synbiohub.org/public/igem/BBa_K574006/annotation2295487/range2295487/1
component: BBa_K574004

https://synbiohub.org/public/igem/BBa_K574006/annotation2295487/range2295487/1
type: Range
persistent identity: range2295487
display id: range2295487
version: 1
direction: direction/reverse
top level: BBa_K574006
start: 1
end: 797
orientation: inline

BBa_K1520003
type: ComponentDefinition
persistent identity: BBa K1520003
display id: BBa_K1520003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Pcons2-rbs-luxR-Ter
creator: yue wu
created: 2014-09-21T11:00:00Z
modified: 2015-05-08T01:10:48Z
discontinued: false
dominant: false
group u list: _1902_
m user id: 0
owner id: 21318
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Pcons2-rbs-luxR-Ter. It achieves a constant expression of luxR, the AHL combining protein Pcons2 is BBa_J23100
mutable notes: NONE
mutable provenance: Pcons2, luxR, Ter
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K1520501
component: BBa_J23100
component: BBa_K1520029
sequence annotation: BBa_K1520501
sequence annotation: BBa_J23100
sequence annotation: BBa_K1520029
sequence: https://synbiohub.org/public/igem/BBa_K1520003_sequence/1

BBa_K1520501
type: Component
persistent identity: component2384887
display id: component2384887
version: 1
top level: BBa_K1520003
access: public
definition: BBa_K1520501

BBa_J23100
type: Component
persistent identity: component2384884
display id: component2384884
version: 1
top level: BBa_K1520003
access: public
definition: https://synbiohub.org/public/igem/BBa_J23100/1

BBa_K1520029
type: Component
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display id: component2384888
version: 1
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access: public
definition: https://synbiohub.org/public/igem/BBa_K1520029/1

BBa_K1520501
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persistent identity: annotation2384887
display id: annotation2384887
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BBa_J23100
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component: BBa_J23100

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BBa_K1520029
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BBa_I0460
type: ComponentDefinition
persistent identity: BBa I0460
display id: BBa_I0460
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: aiiA Device (B0034.C0060.B0015)
creator: Caitlin Conboy and Jennifer Braff
created: 2003-12-04T12:00:00Z
modified: 2015-08-31T04:07:29Z
discontinued: false
dominant: true
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: -- No description --
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0034
component: BBa_B0010
component: BBa_C0060
component: BBa_B0012
sequence annotation: BBa_B0010
sequence annotation: BBa_C0060
sequence annotation: BBa_B0012
sequence annotation: BBa_B0034
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access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

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display id: component943117
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access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_C0060
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access: public
definition: aiiA

BBa_B0012
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access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0010
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BBa_C0060
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BBa_B0034
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BBa_K211001
type: ComponentDefinition
persistent identity: BBa K211001
display id: BBa_K211001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RI7
creator: ZHONG Wanting
created: 2009-10-12T11:00:00Z
modified: 2015-05-08T01:11:29Z
discontinued: false
dominant: false
experience: experience/None
group u list: _313_
m user id: 0
owner id: 4277
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: Complete CDS of Rat olfactory protein mRNA, clone I7.
mutable provenance: Rattus norvegicus.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: stop
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_K211001_sequence/1

stop
type: SequenceAnnotation
persistent identity: annotation2035780
display id: annotation2035780
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role: SO:0000319
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start
type: SequenceAnnotation
persistent identity: annotation2035779
display id: annotation2035779
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top level: BBa_K211001
role: SO:0000318
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persistent identity: range2035779
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start: 1
end: 3
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BBa_K1520512
type: ComponentDefinition
persistent identity: BBa K1520512
display id: BBa_K1520512
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: MC31-rbs-luxI-Ter
creator: yue wu
created: 2014-09-22T11:00:00Z
modified: 2015-05-08T01:10:49Z
discontinued: false
dominant: false
group u list: _1902_
m user id: 0
owner id: 21318
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: MC31-rbs-luxI-Ter If there are microcystin, the luxI will be produced.
mutable notes: None
mutable provenance: MC31, luxI, Ter
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1520504
component: BBa_K1520029
component: BBa_K1520002
sequence annotation: BBa_K1520029
sequence annotation: BBa_K1520002
sequence annotation: BBa_K1520504
sequence: https://synbiohub.org/public/igem/BBa_K1520512_sequence/1

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persistent identity: component2385598
display id: component2385598
version: 1
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access: public
definition: BBa_K1520504

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access: public
definition: https://synbiohub.org/public/igem/BBa_K1520029/1

BBa_K1520002
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persistent identity: component2385595
display id: component2385595
version: 1
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access: public
definition: https://synbiohub.org/public/igem/BBa_K1520002/1

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persistent identity: annotation2385599
display id: annotation2385599
version: 1
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location: https://synbiohub.org/public/igem/BBa_K1520512/annotation2385599/range2385599/1
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direction: direction/reverse
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type: SequenceAnnotation
persistent identity: annotation2385595
display id: annotation2385595
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persistent identity: range2385595
display id: range2385595
version: 1
direction: direction/reverse
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BBa_K1520504
type: SequenceAnnotation
persistent identity: annotation2385598
display id: annotation2385598
version: 1
top level: BBa_K1520512
location: https://synbiohub.org/public/igem/BBa_K1520512/annotation2385598/range2385598/1
component: BBa_K1520504

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type: Range
persistent identity: range2385598
display id: range2385598
version: 1
direction: direction/reverse
top level: BBa_K1520512
start: 141
end: 801
orientation: inline

BBa_I13261
type: ComponentDefinition
persistent identity: BBa I13261
display id: BBa_I13261
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Lux Receiver (I13263 with reversed part order)
creator: Barry Canton
created: 2004-10-30T11:00:00Z
modified: 2015-08-31T04:07:33Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _11_6_
m user id: 0
owner id: 135
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Receiver circuit similar to I13263 but with the order of the reporter and luxR generator reversed
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Signalling
role: SO:0000804
component: BBa_R0062
component: BBa_R0063
component: BBa_C0062
component: BBa_B0034
component: BBa_B0010
component: BBa_E0030
component: BBa_B0012
sequence annotation: BBa_C0062
sequence annotation: BBa_R0063
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_R0062
sequence annotation: BBa_B0034
sequence annotation: BBa_E0030
sequence: https://synbiohub.org/public/igem/BBa_I13261_sequence/1

BBa_R0062
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persistent identity: component1250950
display id: component1250950
version: 1
top level: BBa_I13261
access: public
definition: https://synbiohub.org/public/igem/BBa_R0062/1

BBa_R0063
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persistent identity: component1250992
display id: component1250992
version: 1
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access: public
definition: https://synbiohub.org/public/igem/BBa_R0063/1

BBa_C0062
type: Component
persistent identity: component1251016
display id: component1251016
version: 1
top level: BBa_I13261
access: public
definition: luxr

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persistent identity: component1250958
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version: 1
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access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0010
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persistent identity: component1250965
display id: component1250965
version: 1
top level: BBa_I13261
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_E0030
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persistent identity: component1250960
display id: component1250960
version: 1
top level: BBa_I13261
access: public
definition: https://synbiohub.org/public/igem/BBa_E0030/1

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display id: component1250975
version: 1
top level: BBa_I13261
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0034
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persistent identity: component1251001
display id: component1251001
version: 1
top level: BBa_I13261
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0010
type: Component
persistent identity: component1251024
display id: component1251024
version: 1
top level: BBa_I13261
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
type: Component
persistent identity: component1251034
display id: component1251034
version: 1
top level: BBa_I13261
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_C0062
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persistent identity: annotation1251016
display id: annotation1251016
version: 1
top level: BBa_I13261
location: https://synbiohub.org/public/igem/BBa_I13261/annotation1251016/range1251016/1
component: BBa_C0062

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persistent identity: range1251016
display id: range1251016
version: 1
direction: direction/forward
top level: BBa_I13261
start: 1127
end: 1882
orientation: inline

BBa_R0063
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persistent identity: annotation1250992
display id: annotation1250992
version: 1
top level: BBa_I13261
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persistent identity: range1250992
display id: range1250992
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direction: direction/forward
top level: BBa_I13261
start: 950
end: 1100
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1251024
display id: annotation1251024
version: 1
top level: BBa_I13261
location: https://synbiohub.org/public/igem/BBa_I13261/annotation1251024/range1251024/1
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direction: direction/forward
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end: 1995
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1250975
display id: annotation1250975
version: 1
top level: BBa_I13261
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component: BBa_B0012

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persistent identity: range1250975
display id: range1250975
version: 1
direction: direction/forward
top level: BBa_I13261
start: 901
end: 941
orientation: inline

BBa_B0010
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persistent identity: annotation1250965
display id: annotation1250965
version: 1
top level: BBa_I13261
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component: BBa_B0010

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persistent identity: range1250965
display id: range1250965
version: 1
direction: direction/forward
top level: BBa_I13261
start: 813
end: 892
orientation: inline

BBa_R0062
type: SequenceAnnotation
persistent identity: annotation1250950
display id: annotation1250950
version: 1
top level: BBa_I13261
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component: BBa_R0062

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type: Range
persistent identity: range1250950
display id: range1250950
version: 1
direction: direction/forward
top level: BBa_I13261
start: 1
end: 55
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1251034
display id: annotation1251034
version: 1
top level: BBa_I13261
location: https://synbiohub.org/public/igem/BBa_I13261/annotation1251034/range1251034/1
component: BBa_B0012

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type: Range
persistent identity: range1251034
display id: range1251034
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direction: direction/forward
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end: 2044
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1251001
display id: annotation1251001
version: 1
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location: https://synbiohub.org/public/igem/BBa_I13261/annotation1251001/range1251001/1
component: BBa_B0034

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type: Range
persistent identity: range1251001
display id: range1251001
version: 1
direction: direction/forward
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start: 1109
end: 1120
orientation: inline

BBa_E0030
type: SequenceAnnotation
persistent identity: annotation1250960
display id: annotation1250960
version: 1
top level: BBa_I13261
location: https://synbiohub.org/public/igem/BBa_I13261/annotation1250960/range1250960/1
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persistent identity: range1250960
display id: range1250960
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direction: direction/forward
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start: 82
end: 804
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1250958
display id: annotation1250958
version: 1
top level: BBa_I13261
location: https://synbiohub.org/public/igem/BBa_I13261/annotation1250958/range1250958/1
component: BBa_B0034

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type: Range
persistent identity: range1250958
display id: range1250958
version: 1
direction: direction/forward
top level: BBa_I13261
start: 64
end: 75
orientation: inline

BBa_I13202
type: ComponentDefinition
persistent identity: BBa I13202
display id: BBa_I13202
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: 3OC6HSL Sender Controlled by Lac Repressible Promoter
creator: Barry Canton
created: 2004-07-29T11:00:00Z
modified: 2015-08-31T04:07:33Z
discontinued: false
dominant: true
experience: experience/None
group u list: _101_6_
m user id: 0
owner id: 135
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: A lac repressible promoter driving production of an untagged LuxI enzyme which produces an AHL (3OC6HSL).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Signalling
role: SO:0000804
component: BBa_B0012
component: BBa_B0034
component: BBa_B0010
component: BBa_C0161
component: BBa_R0011
sequence annotation: BBa_C0161
sequence annotation: BBa_B0012
sequence annotation: BBa_R0011
sequence annotation: BBa_B0034
sequence annotation: BBa_B0010
sequence: https://synbiohub.org/public/igem/BBa_I13202_sequence/1

BBa_B0012
type: Component
persistent identity: component1484952
display id: component1484952
version: 1
top level: BBa_I13202
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0034
type: Component
persistent identity: component1484935
display id: component1484935
version: 1
top level: BBa_I13202
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0010
type: Component
persistent identity: component1484942
display id: component1484942
version: 1
top level: BBa_I13202
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_C0161
type: Component
persistent identity: component1484937
display id: component1484937
version: 1
top level: BBa_I13202
access: public
definition: luxI

BBa_R0011
type: Component
persistent identity: component1484926
display id: component1484926
version: 1
top level: BBa_I13202
access: public
definition: https://synbiohub.org/public/igem/BBa_R0011/1

BBa_C0161
type: SequenceAnnotation
persistent identity: annotation1484937
display id: annotation1484937
version: 1
top level: BBa_I13202
location: https://synbiohub.org/public/igem/BBa_I13202/annotation1484937/range1484937/1
component: BBa_C0161

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persistent identity: range1484937
display id: range1484937
version: 1
direction: direction/forward
top level: BBa_I13202
start: 82
end: 666
orientation: inline

BBa_B0012
type: SequenceAnnotation
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display id: annotation1484952
version: 1
top level: BBa_I13202
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component: BBa_B0012

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type: Range
persistent identity: range1484952
display id: range1484952
version: 1
direction: direction/forward
top level: BBa_I13202
start: 763
end: 803
orientation: inline

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation1484926
display id: annotation1484926
version: 1
top level: BBa_I13202
location: https://synbiohub.org/public/igem/BBa_I13202/annotation1484926/range1484926/1
component: BBa_R0011

https://synbiohub.org/public/igem/BBa_I13202/annotation1484926/range1484926/1
type: Range
persistent identity: range1484926
display id: range1484926
version: 1
direction: direction/forward
top level: BBa_I13202
start: 1
end: 54
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1484935
display id: annotation1484935
version: 1
top level: BBa_I13202
location: https://synbiohub.org/public/igem/BBa_I13202/annotation1484935/range1484935/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I13202/annotation1484935/range1484935/1
type: Range
persistent identity: range1484935
display id: range1484935
version: 1
direction: direction/forward
top level: BBa_I13202
start: 64
end: 75
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1484942
display id: annotation1484942
version: 1
top level: BBa_I13202
location: https://synbiohub.org/public/igem/BBa_I13202/annotation1484942/range1484942/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I13202/annotation1484942/range1484942/1
type: Range
persistent identity: range1484942
display id: range1484942
version: 1
direction: direction/forward
top level: BBa_I13202
start: 675
end: 754
orientation: inline

BBa_K116626
type: ComponentDefinition
persistent identity: BBa K116626
display id: BBa_K116626
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: BBa_B0032 + LuxR + BBa_B0015
creator: Jesse Wu
created: 2008-10-30T12:00:00Z
modified: 2015-05-08T01:09:33Z
discontinued: false
dominant: false
experience: experience/None
group u list: _210_
m user id: 0
owner id: 2749
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: RBS + LuxR + T.
mutable notes: none
mutable provenance: ligation
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0015
component: BBa_K116616
sequence annotation: BBa_K116616
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_K116626_sequence/1

BBa_B0015
type: Component
persistent identity: component2249473
display id: component2249473
version: 1
top level: BBa_K116626
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_K116616
type: Component
persistent identity: component2249466
display id: component2249466
version: 1
top level: BBa_K116626
access: public
definition: https://synbiohub.org/public/igem/BBa_K116616/1

BBa_K116616
type: SequenceAnnotation
persistent identity: annotation2249466
display id: annotation2249466
version: 1
top level: BBa_K116626
location: https://synbiohub.org/public/igem/BBa_K116626/annotation2249466/range2249466/1
component: BBa_K116616

https://synbiohub.org/public/igem/BBa_K116626/annotation2249466/range2249466/1
type: Range
persistent identity: range2249466
display id: range2249466
version: 1
direction: direction/reverse
top level: BBa_K116626
start: 1
end: 800
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2249473
display id: annotation2249473
version: 1
top level: BBa_K116626
location: https://synbiohub.org/public/igem/BBa_K116626/annotation2249473/range2249473/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K116626/annotation2249473/range2249473/1
type: Range
persistent identity: range2249473
display id: range2249473
version: 1
direction: direction/reverse
top level: BBa_K116626
start: 809
end: 937
orientation: inline

QS->RFP
type: ComponentDefinition
persistent identity: BBa J85120
display id: BBa_J85120
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: High Population->mRFP (pLac->LuxI; pLacIQ->luxR; pLux->mRFP;)
creator: Cory Li
created: 2010-05-31T11:00:00Z
modified: 2015-05-08T01:08:29Z
discontinued: false
dominant: false
experience: experience/None
group u list: _406_
m user id: 0
owner id: 2073
owning group id: 387
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Quorum sensing circuit under a pLac promoter. Population turns red (mRFP) once quorum sensing kicks in.
mutable notes: Double terminator not used after LuxI because it is unnecessary and has the potential to make the plasmid more unstable.
mutable provenance: Built through assembly of BBa_K084014 and BBa_J10000.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K084014
component: BBa_J10000
sequence annotation: BBa_J10000
sequence annotation: BBa_K084014
sequence: https://synbiohub.org/public/igem/BBa_J85120_sequence/1

BBa_K084014
type: Component
persistent identity: component2294800
display id: component2294800
version: 1
top level: QS->RFP
access: public
definition: https://synbiohub.org/public/igem/BBa_K084014/1

BBa_J10000
type: Component
persistent identity: component2294832
display id: component2294832
version: 1
top level: QS->RFP
access: public
definition: https://synbiohub.org/public/igem/BBa_J10000/1

BBa_J10000
type: SequenceAnnotation
persistent identity: annotation2294832
display id: annotation2294832
version: 1
top level: QS->RFP
location: https://synbiohub.org/public/igem/BBa_J85120/annotation2294832/range2294832/1
component: BBa_J10000

https://synbiohub.org/public/igem/BBa_J85120/annotation2294832/range2294832/1
type: Range
persistent identity: range2294832
display id: range2294832
version: 1
direction: direction/reverse
top level: QS->RFP
start: 878
end: 2653
orientation: inline

BBa_K084014
type: SequenceAnnotation
persistent identity: annotation2294800
display id: annotation2294800
version: 1
top level: QS->RFP
location: https://synbiohub.org/public/igem/BBa_J85120/annotation2294800/range2294800/1
component: BBa_K084014

https://synbiohub.org/public/igem/BBa_J85120/annotation2294800/range2294800/1
type: Range
persistent identity: range2294800
display id: range2294800
version: 1
direction: direction/reverse
top level: QS->RFP
start: 1
end: 869
orientation: inline

BBa_K1520504
type: ComponentDefinition
persistent identity: BBa K1520504
display id: BBa_K1520504
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Rbs-luxI(scarless)
creator: yue wu
created: 2014-09-21T11:00:00Z
modified: 2015-05-08T01:10:49Z
discontinued: false
dominant: false
group u list: _1902_
m user id: 0
owner id: 21318
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Rbs-luxI(scarless) It is an improvement of luxI(BBa_C0061) by adding a rbs.
mutable notes: None
mutable provenance: It is an improvement of luxI(BBa_C0061) by adding a rbs.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
sequence annotation: rbs
sequence annotation: luxI
sequence: https://synbiohub.org/public/igem/BBa_K1520504_sequence/1

rbs
type: SequenceAnnotation
persistent identity: annotation2384869
display id: annotation2384869
version: 1
top level: BBa_K1520504
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1520504/annotation2384869/range2384869/1

https://synbiohub.org/public/igem/BBa_K1520504/annotation2384869/range2384869/1
type: Range
persistent identity: range2384869
display id: range2384869
version: 1
direction: direction/forward
top level: BBa_K1520504
start: 1
end: 18
orientation: inline

luxI
type: SequenceAnnotation
persistent identity: annotation2384870
display id: annotation2384870
version: 1
top level: BBa_K1520504
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1520504/annotation2384870/range2384870/1

https://synbiohub.org/public/igem/BBa_K1520504/annotation2384870/range2384870/1
type: Range
persistent identity: range2384870
display id: range2384870
version: 1
direction: direction/forward
top level: BBa_K1520504
start: 19
end: 661
orientation: inline

BBa_K094106
type: ComponentDefinition
persistent identity: BBa K094106
display id: BBa_K094106
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Plux-rbs-CI-terminator-plambda P(O-R12)-rbs-cheZ
creator: Liu Chenli
created: 2008-10-13T11:00:00Z
modified: 2015-05-08T01:08:40Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _255_
m user id: 0
owner id: 2589
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This is a part in which the expression of cheZ is repressed by increasing AHL concentration in the environment in the presence of luxR protein. In other circumstances the cheZ gene is constitutively expressed. The cheZ gene lacks a terminator.
mutable notes: N/A
mutable provenance: composited from K094101 and K094160
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_B0034
component: BBa_R0062
component: BBa_K094011
component: BBa_K094100
component: BBa_K094002
component: BBa_K094013
sequence annotation: BBa_K094002
sequence annotation: BBa_R0062
sequence annotation: BBa_K094100
sequence annotation: BBa_B0034
sequence annotation: BBa_K094011
sequence annotation: BBa_K094013
sequence: https://synbiohub.org/public/igem/BBa_K094106_sequence/1

BBa_B0034
type: Component
persistent identity: component1991531
display id: component1991531
version: 1
top level: BBa_K094106
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_R0062
type: Component
persistent identity: component1991520
display id: component1991520
version: 1
top level: BBa_K094106
access: public
definition: https://synbiohub.org/public/igem/BBa_R0062/1

BBa_K094011
type: Component
persistent identity: component1991526
display id: component1991526
version: 1
top level: BBa_K094106
access: public
definition: https://synbiohub.org/public/igem/BBa_K094011/1

BBa_K094100
type: Component
persistent identity: component1991533
display id: component1991533
version: 1
top level: BBa_K094106
access: public
definition: https://synbiohub.org/public/igem/BBa_K094100/1

BBa_K094002
type: Component
persistent identity: component1991529
display id: component1991529
version: 1
top level: BBa_K094106
access: public
definition: https://synbiohub.org/public/igem/BBa_K094002/1

BBa_K094013
type: Component
persistent identity: component1991527
display id: component1991527
version: 1
top level: BBa_K094106
access: public
definition: https://synbiohub.org/public/igem/BBa_K094013/1

BBa_B0034
type: Component
persistent identity: component1991525
display id: component1991525
version: 1
top level: BBa_K094106
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K094002
type: SequenceAnnotation
persistent identity: annotation1991529
display id: annotation1991529
version: 1
top level: BBa_K094106
location: https://synbiohub.org/public/igem/BBa_K094106/annotation1991529/range1991529/1
component: BBa_K094002

https://synbiohub.org/public/igem/BBa_K094106/annotation1991529/range1991529/1
type: Range
persistent identity: range1991529
display id: range1991529
version: 1
direction: direction/reverse
top level: BBa_K094106
start: 1701
end: 1800
orientation: inline

BBa_R0062
type: SequenceAnnotation
persistent identity: annotation1991520
display id: annotation1991520
version: 1
top level: BBa_K094106
location: https://synbiohub.org/public/igem/BBa_K094106/annotation1991520/range1991520/1
component: BBa_R0062

https://synbiohub.org/public/igem/BBa_K094106/annotation1991520/range1991520/1
type: Range
persistent identity: range1991520
display id: range1991520
version: 1
direction: direction/forward
top level: BBa_K094106
start: 1
end: 55
orientation: inline

BBa_K094100
type: SequenceAnnotation
persistent identity: annotation1991533
display id: annotation1991533
version: 1
top level: BBa_K094106
location: https://synbiohub.org/public/igem/BBa_K094106/annotation1991533/range1991533/1
component: BBa_K094100

https://synbiohub.org/public/igem/BBa_K094106/annotation1991533/range1991533/1
type: Range
persistent identity: range1991533
display id: range1991533
version: 1
direction: direction/reverse
top level: BBa_K094106
start: 1827
end: 2521
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1991531
display id: annotation1991531
version: 1
top level: BBa_K094106
location: https://synbiohub.org/public/igem/BBa_K094106/annotation1991531/range1991531/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K094106/annotation1991531/range1991531/1
type: Range
persistent identity: range1991531
display id: range1991531
version: 1
direction: direction/reverse
top level: BBa_K094106
start: 1809
end: 1820
orientation: inline

BBa_K094011
type: SequenceAnnotation
persistent identity: annotation1991526
display id: annotation1991526
version: 1
top level: BBa_K094106
location: https://synbiohub.org/public/igem/BBa_K094106/annotation1991526/range1991526/1
component: BBa_K094011

https://synbiohub.org/public/igem/BBa_K094106/annotation1991526/range1991526/1
type: Range
persistent identity: range1991526
display id: range1991526
version: 1
direction: direction/reverse
top level: BBa_K094106
start: 82
end: 1590
orientation: inline

BBa_K094013
type: SequenceAnnotation
persistent identity: annotation1991527
display id: annotation1991527
version: 1
top level: BBa_K094106
location: https://synbiohub.org/public/igem/BBa_K094106/annotation1991527/range1991527/1
component: BBa_K094013

https://synbiohub.org/public/igem/BBa_K094106/annotation1991527/range1991527/1
type: Range
persistent identity: range1991527
display id: range1991527
version: 1
direction: direction/reverse
top level: BBa_K094106
start: 1599
end: 1692
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1991525
display id: annotation1991525
version: 1
top level: BBa_K094106
location: https://synbiohub.org/public/igem/BBa_K094106/annotation1991525/range1991525/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K094106/annotation1991525/range1991525/1
type: Range
persistent identity: range1991525
display id: range1991525
version: 1
direction: direction/reverse
top level: BBa_K094106
start: 64
end: 75
orientation: inline

BBa_K574004
type: ComponentDefinition
persistent identity: BBa K574004
display id: BBa_K574004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: 3OC12HSL regulated by TetR
creator: Lei Wei
created: 2011-09-28T11:00:00Z
modified: 2015-05-08T01:12:44Z
discontinued: false
dominant: false
experience: experience/None
group u list: _745_
m user id: 0
owner id: 8529
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: p(tetR) + lasI
mutable notes: Empty.
mutable provenance: Existing Parts.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Signalling
role: SO:0000804
component: BBa_K081016
component: BBa_R0040
sequence annotation: BBa_K081016
sequence annotation: BBa_R0040
sequence: https://synbiohub.org/public/igem/BBa_K574004_sequence/1

BBa_K081016
type: Component
persistent identity: component2250774
display id: component2250774
version: 1
top level: BBa_K574004
access: public
definition: https://synbiohub.org/public/igem/BBa_K081016/1

BBa_R0040
type: Component
persistent identity: component2250757
display id: component2250757
version: 1
top level: BBa_K574004
access: public
definition: https://synbiohub.org/public/igem/BBa_R0040/1

BBa_K081016
type: SequenceAnnotation
persistent identity: annotation2250774
display id: annotation2250774
version: 1
top level: BBa_K574004
location: https://synbiohub.org/public/igem/BBa_K574004/annotation2250774/range2250774/1
component: BBa_K081016

https://synbiohub.org/public/igem/BBa_K574004/annotation2250774/range2250774/1
type: Range
persistent identity: range2250774
display id: range2250774
version: 1
direction: direction/reverse
top level: BBa_K574004
start: 63
end: 797
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2250757
display id: annotation2250757
version: 1
top level: BBa_K574004
location: https://synbiohub.org/public/igem/BBa_K574004/annotation2250757/range2250757/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_K574004/annotation2250757/range2250757/1
type: Range
persistent identity: range2250757
display id: range2250757
version: 1
direction: direction/forward
top level: BBa_K574004
start: 1
end: 54
orientation: inline

BBa_K081011
type: ComponentDefinition
persistent identity: BBa K081011
display id: BBa_K081011
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: luxR protein generator under Plambda regulation (TERM-)
creator: Lorenzo Pasotti, Paolo Magni
created: 2008-10-17T11:00:00Z
modified: 2015-05-08T01:08:35Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _227_
m user id: 0
owner id: 2583
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: luxR protein generator without transcriptional terminator. This generator is under Plambda repressible promoter. Strong RBS (efficiency=0.6).
mutable notes: We used BioBrick Standard Assembly.
mutable provenance: Plambda: RBS: luxR:
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_C0062
component: BBa_B0030
component: BBa_R0051
sequence annotation: BBa_R0051
sequence annotation: BBa_C0062
sequence annotation: BBa_B0030
sequence: https://synbiohub.org/public/igem/BBa_K081011_sequence/1

BBa_C0062
type: Component
persistent identity: component2246131
display id: component2246131
version: 1
top level: BBa_K081011
access: public
definition: luxr

BBa_B0030
type: Component
persistent identity: component2246127
display id: component2246127
version: 1
top level: BBa_K081011
access: public
definition: https://synbiohub.org/public/igem/BBa_B0030/1

BBa_R0051
type: Component
persistent identity: component2246122
display id: component2246122
version: 1
top level: BBa_K081011
access: public
definition: https://synbiohub.org/public/igem/BBa_R0051/1

BBa_R0051
type: SequenceAnnotation
persistent identity: annotation2246122
display id: annotation2246122
version: 1
top level: BBa_K081011
location: https://synbiohub.org/public/igem/BBa_K081011/annotation2246122/range2246122/1
component: BBa_R0051

https://synbiohub.org/public/igem/BBa_K081011/annotation2246122/range2246122/1
type: Range
persistent identity: range2246122
display id: range2246122
version: 1
direction: direction/forward
top level: BBa_K081011
start: 1
end: 49
orientation: inline

BBa_C0062
type: SequenceAnnotation
persistent identity: annotation2246131
display id: annotation2246131
version: 1
top level: BBa_K081011
location: https://synbiohub.org/public/igem/BBa_K081011/annotation2246131/range2246131/1
component: BBa_C0062

https://synbiohub.org/public/igem/BBa_K081011/annotation2246131/range2246131/1
type: Range
persistent identity: range2246131
display id: range2246131
version: 1
direction: direction/forward
top level: BBa_K081011
start: 79
end: 834
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation2246127
display id: annotation2246127
version: 1
top level: BBa_K081011
location: https://synbiohub.org/public/igem/BBa_K081011/annotation2246127/range2246127/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_K081011/annotation2246127/range2246127/1
type: Range
persistent identity: range2246127
display id: range2246127
version: 1
direction: direction/forward
top level: BBa_K081011
start: 58
end: 72
orientation: inline

BBa_J07044
type: ComponentDefinition
persistent identity: BBa J07044
display id: BBa_J07044
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: J07013.B0015 (scFv S101A.TT)
creator: Jenny Nguyen
created: 2005-08-02T11:00:00Z
modified: 2015-08-31T04:08:20Z
discontinued: false
dominant: false
group u list: _21_
m user id: 0
owner id: 350
owning group id: 21
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Anti-fluorescein scFv (one mutation, S101A) with transcriptional terminator.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Signalling
role: SO:0000804
component: BBa_B0012
component: BBa_J07013
component: BBa_B0010
sequence annotation: BBa_J07013
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_J07044_sequence/1

BBa_B0012
type: Component
persistent identity: component1651250
display id: component1651250
version: 1
top level: BBa_J07044
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_J07013
type: Component
persistent identity: component1651235
display id: component1651235
version: 1
top level: BBa_J07044
access: public
definition: BBa_J07013

BBa_B0010
type: Component
persistent identity: component1651240
display id: component1651240
version: 1
top level: BBa_J07044
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_J07013
type: SequenceAnnotation
persistent identity: annotation1651235
display id: annotation1651235
version: 1
top level: BBa_J07044
location: https://synbiohub.org/public/igem/BBa_J07044/annotation1651235/range1651235/1
component: BBa_J07013

https://synbiohub.org/public/igem/BBa_J07044/annotation1651235/range1651235/1
type: Range
persistent identity: range1651235
display id: range1651235
version: 1
direction: direction/forward
top level: BBa_J07044
start: 1
end: 762
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1651240
display id: annotation1651240
version: 1
top level: BBa_J07044
location: https://synbiohub.org/public/igem/BBa_J07044/annotation1651240/range1651240/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_J07044/annotation1651240/range1651240/1
type: Range
persistent identity: range1651240
display id: range1651240
version: 1
direction: direction/forward
top level: BBa_J07044
start: 771
end: 850
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1651250
display id: annotation1651250
version: 1
top level: BBa_J07044
location: https://synbiohub.org/public/igem/BBa_J07044/annotation1651250/range1651250/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_J07044/annotation1651250/range1651250/1
type: Range
persistent identity: range1651250
display id: range1651250
version: 1
direction: direction/forward
top level: BBa_J07044
start: 859
end: 899
orientation: inline

BBa_I13206
type: ComponentDefinition
persistent identity: BBa I13206
display id: BBa_I13206
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: aiiA (LVA-) protein generator driven by ptet
creator: Barry Canton
created: 2004-07-29T11:00:00Z
modified: 2015-08-31T04:07:33Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_6_
m user id: 0
owner id: 135
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Produces aiiA protein from the tet promoter. The aiiA is untagged and should be capable of degrading any intracellular AHL.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Signalling
role: SO:0000804
component: BBa_B0034
component: BBa_R0040
component: BBa_B0010
component: BBa_C0160
component: BBa_B0012
sequence annotation: BBa_C0160
sequence annotation: BBa_B0010
sequence annotation: BBa_R0040
sequence annotation: BBa_B0012
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_I13206_sequence/1

BBa_B0034
type: Component
persistent identity: component1251292
display id: component1251292
version: 1
top level: BBa_I13206
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_R0040
type: Component
persistent identity: component1251284
display id: component1251284
version: 1
top level: BBa_I13206
access: public
definition: https://synbiohub.org/public/igem/BBa_R0040/1

BBa_B0010
type: Component
persistent identity: component1251299
display id: component1251299
version: 1
top level: BBa_I13206
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_C0160
type: Component
persistent identity: component1251294
display id: component1251294
version: 1
top level: BBa_I13206
access: public
definition: aiiA

BBa_B0012
type: Component
persistent identity: component1251309
display id: component1251309
version: 1
top level: BBa_I13206
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_C0160
type: SequenceAnnotation
persistent identity: annotation1251294
display id: annotation1251294
version: 1
top level: BBa_I13206
location: https://synbiohub.org/public/igem/BBa_I13206/annotation1251294/range1251294/1
component: BBa_C0160

https://synbiohub.org/public/igem/BBa_I13206/annotation1251294/range1251294/1
type: Range
persistent identity: range1251294
display id: range1251294
version: 1
direction: direction/forward
top level: BBa_I13206
start: 81
end: 836
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1251299
display id: annotation1251299
version: 1
top level: BBa_I13206
location: https://synbiohub.org/public/igem/BBa_I13206/annotation1251299/range1251299/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I13206/annotation1251299/range1251299/1
type: Range
persistent identity: range1251299
display id: range1251299
version: 1
direction: direction/forward
top level: BBa_I13206
start: 845
end: 924
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1251284
display id: annotation1251284
version: 1
top level: BBa_I13206
location: https://synbiohub.org/public/igem/BBa_I13206/annotation1251284/range1251284/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_I13206/annotation1251284/range1251284/1
type: Range
persistent identity: range1251284
display id: range1251284
version: 1
direction: direction/forward
top level: BBa_I13206
start: 1
end: 54
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1251309
display id: annotation1251309
version: 1
top level: BBa_I13206
location: https://synbiohub.org/public/igem/BBa_I13206/annotation1251309/range1251309/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I13206/annotation1251309/range1251309/1
type: Range
persistent identity: range1251309
display id: range1251309
version: 1
direction: direction/forward
top level: BBa_I13206
start: 933
end: 973
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1251292
display id: annotation1251292
version: 1
top level: BBa_I13206
location: https://synbiohub.org/public/igem/BBa_I13206/annotation1251292/range1251292/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I13206/annotation1251292/range1251292/1
type: Range
persistent identity: range1251292
display id: range1251292
version: 1
direction: direction/forward
top level: BBa_I13206
start: 63
end: 74
orientation: inline

BBa_C0260
type: ComponentDefinition
persistent identity: BBa C0260
display id: BBa_C0260
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: autoinducer inactivation enzyme, AaiiA (+RBS)
creator: robertb
created: 2004-08-22T11:00:00Z
modified: 2015-08-31T04:07:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _6_
m user id: 0
owner id: 159
owning group id: 7
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: B0034.C0060
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_C0060
component: BBa_B0034
sequence annotation: BBa_C0060
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_C0260_sequence/1

BBa_C0060
type: Component
persistent identity: component1060397
display id: component1060397
version: 1
top level: BBa_C0260
access: public
definition: aiiA

BBa_B0034
type: Component
persistent identity: component1060382
display id: component1060382
version: 1
top level: BBa_C0260
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_C0060
type: SequenceAnnotation
persistent identity: annotation1060397
display id: annotation1060397
version: 1
top level: BBa_C0260
location: https://synbiohub.org/public/igem/BBa_C0260/annotation1060397/range1060397/1
component: BBa_C0060

https://synbiohub.org/public/igem/BBa_C0260/annotation1060397/range1060397/1
type: Range
persistent identity: range1060397
display id: range1060397
version: 1
direction: direction/forward
top level: BBa_C0260
start: 19
end: 807
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1060382
display id: annotation1060382
version: 1
top level: BBa_C0260
location: https://synbiohub.org/public/igem/BBa_C0260/annotation1060382/range1060382/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_C0260/annotation1060382/range1060382/1
type: Range
persistent identity: range1060382
display id: range1060382
version: 1
direction: direction/forward
top level: BBa_C0260
start: 1
end: 12
orientation: inline

BBa_J06401
type: ComponentDefinition
persistent identity: BBa J06401
display id: BBa_J06401
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: 30C12HSL Sender
creator: kxjin
created: 2005-06-21T11:00:00Z
modified: 2015-08-31T04:08:17Z
discontinued: false
dominant: true
experience: experience/None
group u list: _20_
m user id: 0
owner id: 368
owning group id: 20
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This protein generator produces the enzyme LasI. This enzyme produces 3OC12HSL. This is the 3OC12HSL analog to part F1611.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Signalling
role: SO:0000804
component: BBa_B0010
component: BBa_C0178
component: BBa_B0034
component: BBa_B0012
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_C0178
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_J06401_sequence/1

BBa_B0010
type: Component
persistent identity: component1532339
display id: component1532339
version: 1
top level: BBa_J06401
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_C0178
type: Component
persistent identity: component1532334
display id: component1532334
version: 1
top level: BBa_J06401
access: public
definition: lasI

BBa_B0034
type: Component
persistent identity: component1532332
display id: component1532332
version: 1
top level: BBa_J06401
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0012
type: Component
persistent identity: component1532349
display id: component1532349
version: 1
top level: BBa_J06401
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1532339
display id: annotation1532339
version: 1
top level: BBa_J06401
location: https://synbiohub.org/public/igem/BBa_J06401/annotation1532339/range1532339/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_J06401/annotation1532339/range1532339/1
type: Range
persistent identity: range1532339
display id: range1532339
version: 1
direction: direction/forward
top level: BBa_J06401
start: 636
end: 715
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1532349
display id: annotation1532349
version: 1
top level: BBa_J06401
location: https://synbiohub.org/public/igem/BBa_J06401/annotation1532349/range1532349/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_J06401/annotation1532349/range1532349/1
type: Range
persistent identity: range1532349
display id: range1532349
version: 1
direction: direction/forward
top level: BBa_J06401
start: 724
end: 764
orientation: inline

BBa_C0178
type: SequenceAnnotation
persistent identity: annotation1532334
display id: annotation1532334
version: 1
top level: BBa_J06401
location: https://synbiohub.org/public/igem/BBa_J06401/annotation1532334/range1532334/1
component: BBa_C0178

https://synbiohub.org/public/igem/BBa_J06401/annotation1532334/range1532334/1
type: Range
persistent identity: range1532334
display id: range1532334
version: 1
direction: direction/forward
top level: BBa_J06401
start: 19
end: 627
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1532332
display id: annotation1532332
version: 1
top level: BBa_J06401
location: https://synbiohub.org/public/igem/BBa_J06401/annotation1532332/range1532332/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_J06401/annotation1532332/range1532332/1
type: Range
persistent identity: range1532332
display id: range1532332
version: 1
direction: direction/forward
top level: BBa_J06401
start: 1
end: 12
orientation: inline

BBa_K1897003
type: ComponentDefinition
persistent identity: BBa K1897003
display id: BBa_K1897003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: HasS antisigma factor coding sequence
creator: Choi Yan Ru
created: 2016-10-08T11:00:00Z
modified: 2016-10-18T01:29:52Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2363_
m user id: 29428
owner id: 29428
owning group id: 9
status: status/Planning
bookmark: false
mutable description: HasR is the membrane-bound receptor in the Has system, a heme acquisition system originally from the Serratia marcescens. The HasR protein is involved in binding with a heme-loaded HasA hemophore to trigger downstream HasS and HasI. It has been shown that the N terminal of HasR is especially crucial for signal transduction. With the heme-loaded hasA bound to hasR, it induces a conformational change in HasR that causes the activation of HasS, an anti-sigma factor. HasS then releases HasI (sigma factor) that was initially bound to it and HasI ultimately is able to trigger expression of genes under control of pHas (part number).
mutable notes: NIL
mutable provenance: The HasS gene is originally found in the Serratia marcescens. We obtained the gene sequence from the National Center for Biotechnology Information (NCBI) database (http://www.ncbi.nlm.nih.gov/nuccore/X81195.2) and synthesised the gene.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1897003_sequence/1

BBa_K094103
type: ComponentDefinition
persistent identity: BBa K094103
display id: BBa_K094103
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: plux-rbs-cheZ-terminator
creator: Liu Chenli
created: 2008-10-13T11:00:00Z
modified: 2015-05-08T01:08:40Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _255_
m user id: 0
owner id: 2589
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This is a cheZ gene that can be induced by luxR protein under high AHL concentration. cheZ protein is responsible for the dephosphorylation of cheY protein in bacteria flagella movement.
mutable notes: N/A
mutable provenance: composited from E. Coli cheZ gene and lux promoter
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_R0062
component: BBa_K094100
component: BBa_K094013
component: BBa_B0034
sequence annotation: BBa_R0062
sequence annotation: BBa_K094013
sequence annotation: BBa_B0034
sequence annotation: BBa_K094100
sequence: https://synbiohub.org/public/igem/BBa_K094103_sequence/1

BBa_R0062
type: Component
persistent identity: component1990867
display id: component1990867
version: 1
top level: BBa_K094103
access: public
definition: https://synbiohub.org/public/igem/BBa_R0062/1

BBa_K094100
type: Component
persistent identity: component1990874
display id: component1990874
version: 1
top level: BBa_K094103
access: public
definition: https://synbiohub.org/public/igem/BBa_K094100/1

BBa_K094013
type: Component
persistent identity: component1990875
display id: component1990875
version: 1
top level: BBa_K094103
access: public
definition: https://synbiohub.org/public/igem/BBa_K094013/1

BBa_B0034
type: Component
persistent identity: component1990872
display id: component1990872
version: 1
top level: BBa_K094103
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_R0062
type: SequenceAnnotation
persistent identity: annotation1990867
display id: annotation1990867
version: 1
top level: BBa_K094103
location: https://synbiohub.org/public/igem/BBa_K094103/annotation1990867/range1990867/1
component: BBa_R0062

https://synbiohub.org/public/igem/BBa_K094103/annotation1990867/range1990867/1
type: Range
persistent identity: range1990867
display id: range1990867
version: 1
direction: direction/forward
top level: BBa_K094103
start: 1
end: 55
orientation: inline

BBa_K094013
type: SequenceAnnotation
persistent identity: annotation1990875
display id: annotation1990875
version: 1
top level: BBa_K094103
location: https://synbiohub.org/public/igem/BBa_K094103/annotation1990875/range1990875/1
component: BBa_K094013

https://synbiohub.org/public/igem/BBa_K094103/annotation1990875/range1990875/1
type: Range
persistent identity: range1990875
display id: range1990875
version: 1
direction: direction/reverse
top level: BBa_K094103
start: 785
end: 878
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1990872
display id: annotation1990872
version: 1
top level: BBa_K094103
location: https://synbiohub.org/public/igem/BBa_K094103/annotation1990872/range1990872/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K094103/annotation1990872/range1990872/1
type: Range
persistent identity: range1990872
display id: range1990872
version: 1
direction: direction/reverse
top level: BBa_K094103
start: 64
end: 75
orientation: inline

BBa_K094100
type: SequenceAnnotation
persistent identity: annotation1990874
display id: annotation1990874
version: 1
top level: BBa_K094103
location: https://synbiohub.org/public/igem/BBa_K094103/annotation1990874/range1990874/1
component: BBa_K094100

https://synbiohub.org/public/igem/BBa_K094103/annotation1990874/range1990874/1
type: Range
persistent identity: range1990874
display id: range1990874
version: 1
direction: direction/reverse
top level: BBa_K094103
start: 82
end: 776
orientation: inline

luxI
type: ComponentDefinition
persistent identity: BBa C0161
display id: BBa_C0161
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: autoinducer synthetase for AHL (no LVA)
creator: jcbraff
created: 2004-05-26T11:00:00Z
modified: 2015-08-31T04:07:24Z
discontinued: false
dominant: true
experience: experience/None
group u list: _11_1_
m user id: 0
owner id: 61
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: same as C0061 except no LVA tag
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: LuxI
sequence: https://synbiohub.org/public/igem/BBa_C0161_sequence/1

LuxI
type: SequenceAnnotation
persistent identity: annotation1891615
display id: annotation1891615
version: 1
top level: luxI
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_C0161/annotation1891615/range1891615/1

https://synbiohub.org/public/igem/BBa_C0161/annotation1891615/range1891615/1
type: Range
persistent identity: range1891615
display id: range1891615
version: 1
direction: direction/forward
top level: luxI
start: 1
end: 585
orientation: inline

BBa_K1631025
type: ComponentDefinition
persistent identity: BBa K1631025
display id: BBa_K1631025
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Colicin-E3 cassette (Medium)
creator: Yuto Yamanaka
created: 2015-09-13T11:00:00Z
modified: 2015-09-18T09:38:28Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2048_
m user id: 23567
owner id: 23567
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Colicin-E3, its immunity protein, and colisin lysis protein is constitutively expressed.(under BBa_J23110) When this device works, Colicin-E3 is released to media, and kill E. coli strains which does not have immunity protein.
mutable notes: none
mutable provenance: Assembly
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_J23110
component: BBa_K1631009
sequence annotation: BBa_J23110
sequence annotation: BBa_K1631009
sequence: https://synbiohub.org/public/igem/BBa_K1631025_sequence/1

BBa_J23110
type: Component
persistent identity: component2455808
display id: component2455808
version: 1
top level: BBa_K1631025
access: public
definition: https://synbiohub.org/public/igem/BBa_J23110/1

BBa_K1631009
type: Component
persistent identity: component2455825
display id: component2455825
version: 1
top level: BBa_K1631025
access: public
definition: https://synbiohub.org/public/igem/BBa_K1631009/1

BBa_J23110
type: SequenceAnnotation
persistent identity: annotation2455808
display id: annotation2455808
version: 1
top level: BBa_K1631025
location: https://synbiohub.org/public/igem/BBa_K1631025/annotation2455808/range2455808/1
component: BBa_J23110

https://synbiohub.org/public/igem/BBa_K1631025/annotation2455808/range2455808/1
type: Range
persistent identity: range2455808
display id: range2455808
version: 1
direction: direction/reverse
top level: BBa_K1631025
start: 1
end: 35
orientation: inline

BBa_K1631009
type: SequenceAnnotation
persistent identity: annotation2455825
display id: annotation2455825
version: 1
top level: BBa_K1631025
location: https://synbiohub.org/public/igem/BBa_K1631025/annotation2455825/range2455825/1
component: BBa_K1631009

https://synbiohub.org/public/igem/BBa_K1631025/annotation2455825/range2455825/1
type: Range
persistent identity: range2455825
display id: range2455825
version: 1
direction: direction/reverse
top level: BBa_K1631025
start: 44
end: 2290
orientation: inline

BBa_K1520023
type: ComponentDefinition
persistent identity: BBa K1520023
display id: BBa_K1520023
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Pcons2-rbs-lacI-Ter-Plac-rbs-luxI-Ter-Pcons2-rbs-luxR-Ter-Prlux-rbs-rfp-rbs-luxR-Ter
creator: yue wu
created: 2014-09-21T11:00:00Z
modified: 2015-05-08T01:10:49Z
discontinued: false
dominant: false
group u list: _1902_
m user id: 0
owner id: 21318
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Plasmid in positive feedback verification: Pcons2-rbs-lacI-Ter-Plac-rbs-luxI-Ter-Pcons2-rbs-luxR-Ter-Prlux-rbs-rfp-rbs-luxR-Ter. It is a positive feedback plasmid using luxR as key to feedback. Introducing IPTG will produce rfp and luxR, and luxR will work back to enhance production of rfp.
mutable notes: None
mutable provenance: Pcons2, lacI, Plac, luxI, luxR, Prlux, rfp, Ter
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1520020
component: BBa_K1520012
sequence annotation: BBa_K1520012
sequence annotation: BBa_K1520020
sequence: https://synbiohub.org/public/igem/BBa_K1520023_sequence/1

BBa_K1520020
type: Component
persistent identity: component2385213
display id: component2385213
version: 1
top level: BBa_K1520023
access: public
definition: BBa_K1520020

BBa_K1520012
type: Component
persistent identity: component2385224
display id: component2385224
version: 1
top level: BBa_K1520023
access: public
definition: BBa_K1520012

BBa_K1520012
type: SequenceAnnotation
persistent identity: annotation2385224
display id: annotation2385224
version: 1
top level: BBa_K1520023
location: https://synbiohub.org/public/igem/BBa_K1520023/annotation2385224/range2385224/1
component: BBa_K1520012

https://synbiohub.org/public/igem/BBa_K1520023/annotation2385224/range2385224/1
type: Range
persistent identity: range2385224
display id: range2385224
version: 1
direction: direction/reverse
top level: BBa_K1520023
start: 3361
end: 5099
orientation: inline

BBa_K1520020
type: SequenceAnnotation
persistent identity: annotation2385213
display id: annotation2385213
version: 1
top level: BBa_K1520023
location: https://synbiohub.org/public/igem/BBa_K1520023/annotation2385213/range2385213/1
component: BBa_K1520020

https://synbiohub.org/public/igem/BBa_K1520023/annotation2385213/range2385213/1
type: Range
persistent identity: range2385213
display id: range2385213
version: 1
direction: direction/reverse
top level: BBa_K1520023
start: 1
end: 3352
orientation: inline

BBa_K1897002
type: ComponentDefinition
persistent identity: BBa K1897002
display id: BBa_K1897002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: HasR Membrane receptor coding sequence
creator: Choi Yan Ru
created: 2016-10-08T11:00:00Z
modified: 2016-10-09T02:50:37Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2363_
m user id: 29428
owner id: 29428
owning group id: 9
status: status/Planning
bookmark: false
mutable description: HasR is the membrane-bound receptor in the Has system, a heme acquisition system originally from the Serratia marcescens. The HasR protein is involved in binding with a heme-loaded HasA hemophore to trigger downstream HasS and HasI. It has been shown that the N terminal of HasR is especially crucial for signal transduction. With the heme-loaded hasA bound to hasR, it induces a conformational change in HasR that causes the activation of HasS, an anti-sigma factor. HasS then releases HasI (sigma factor) that was initially bound to it and HasI ultimately is able to trigger expression of genes under control of pHas (part number).
mutable notes: NIL
mutable provenance: The HasR gene is originally found in the Serratia marcescens. We obtained the gene sequence from the National Center for Biotechnology Information (NCBI) database (http://www.ncbi.nlm.nih.gov/nuccore/X81195.2) and synthesised the gene.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: HasR
sequence annotation: Start codon
sequence annotation: Stop codon
sequence: https://synbiohub.org/public/igem/BBa_K1897002_sequence/1

HasR
type: SequenceAnnotation
persistent identity: annotation2489470
display id: annotation2489470
version: 1
top level: BBa_K1897002
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1897002/annotation2489470/range2489470/1

https://synbiohub.org/public/igem/BBa_K1897002/annotation2489470/range2489470/1
type: Range
persistent identity: range2489470
display id: range2489470
version: 1
direction: direction/forward
top level: BBa_K1897002
start: 1
end: 2700
orientation: inline

Start codon
type: SequenceAnnotation
persistent identity: annotation2489469
display id: annotation2489469
version: 1
top level: BBa_K1897002
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1897002/annotation2489469/range2489469/1

https://synbiohub.org/public/igem/BBa_K1897002/annotation2489469/range2489469/1
type: Range
persistent identity: range2489469
display id: range2489469
version: 1
direction: direction/forward
top level: BBa_K1897002
start: 1
end: 3
orientation: inline

Stop codon
type: SequenceAnnotation
persistent identity: annotation2489471
display id: annotation2489471
version: 1
top level: BBa_K1897002
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1897002/annotation2489471/range2489471/1

https://synbiohub.org/public/igem/BBa_K1897002/annotation2489471/range2489471/1
type: Range
persistent identity: range2489471
display id: range2489471
version: 1
direction: direction/reverse
top level: BBa_K1897002
start: 2698
end: 2700
orientation: inline

BBa_I0464
type: ComponentDefinition
persistent identity: BBa I0464
display id: BBa_I0464
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LasR Protein Generator
creator: Fred, Jay, Madeline, Debra
created: 2004-07-12T11:00:00Z
modified: 2015-08-31T04:07:29Z
discontinued: false
dominant: false
experience: experience/None
group u list: _6_
m user id: 0
owner id: 101
owning group id: 7
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Produces LasR protein which can sense AI-1 in the media and activate transcription from R0078, the right hand Lux promoter
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Signalling
role: SO:0000804
component: BBa_B0012
component: BBa_B0034
component: BBa_B0010
component: BBa_C0079
sequence annotation: BBa_B0034
sequence annotation: BBa_C0079
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_I0464_sequence/1

BBa_B0012
type: Component
persistent identity: component943266
display id: component943266
version: 1
top level: BBa_I0464
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0034
type: Component
persistent identity: component943237
display id: component943237
version: 1
top level: BBa_I0464
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0010
type: Component
persistent identity: component943256
display id: component943256
version: 1
top level: BBa_I0464
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_C0079
type: Component
persistent identity: component943250
display id: component943250
version: 1
top level: BBa_I0464
access: public
definition: lasr

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation943237
display id: annotation943237
version: 1
top level: BBa_I0464
location: https://synbiohub.org/public/igem/BBa_I0464/annotation943237/range943237/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I0464/annotation943237/range943237/1
type: Range
persistent identity: range943237
display id: range943237
version: 1
direction: direction/forward
top level: BBa_I0464
start: 1
end: 12
orientation: inline

BBa_C0079
type: SequenceAnnotation
persistent identity: annotation943250
display id: annotation943250
version: 1
top level: BBa_I0464
location: https://synbiohub.org/public/igem/BBa_I0464/annotation943250/range943250/1
component: BBa_C0079

https://synbiohub.org/public/igem/BBa_I0464/annotation943250/range943250/1
type: Range
persistent identity: range943250
display id: range943250
version: 1
direction: direction/forward
top level: BBa_I0464
start: 19
end: 774
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation943256
display id: annotation943256
version: 1
top level: BBa_I0464
location: https://synbiohub.org/public/igem/BBa_I0464/annotation943256/range943256/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I0464/annotation943256/range943256/1
type: Range
persistent identity: range943256
display id: range943256
version: 1
direction: direction/forward
top level: BBa_I0464
start: 808
end: 887
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation943266
display id: annotation943266
version: 1
top level: BBa_I0464
location: https://synbiohub.org/public/igem/BBa_I0464/annotation943266/range943266/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I0464/annotation943266/range943266/1
type: Range
persistent identity: range943266
display id: range943266
version: 1
direction: direction/forward
top level: BBa_I0464
start: 896
end: 936
orientation: inline

BBa_K574009
type: ComponentDefinition
persistent identity: BBa K574009
display id: BBa_K574009
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: 3OC12HSL -> PoPS Receiver
creator: Lei Wei
created: 2011-09-28T11:00:00Z
modified: 2015-05-08T01:12:44Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _745_
m user id: 0
owner id: 8529
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: PoPS -> LasI
mutable notes: Empty.
mutable provenance: Existing Part.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Signalling
role: SO:0000804
component: BBa_K574008
component: BBa_R0079
sequence annotation: BBa_R0079
sequence annotation: BBa_K574008
sequence: https://synbiohub.org/public/igem/BBa_K574009_sequence/1

BBa_K574008
type: Component
persistent identity: component2264352
display id: component2264352
version: 1
top level: BBa_K574009
access: public
definition: https://synbiohub.org/public/igem/BBa_K574008/1

BBa_R0079
type: Component
persistent identity: component2264357
display id: component2264357
version: 1
top level: BBa_K574009
access: public
definition: https://synbiohub.org/public/igem/BBa_R0079/1

BBa_R0079
type: SequenceAnnotation
persistent identity: annotation2264357
display id: annotation2264357
version: 1
top level: BBa_K574009
location: https://synbiohub.org/public/igem/BBa_K574009/annotation2264357/range2264357/1
component: BBa_R0079

https://synbiohub.org/public/igem/BBa_K574009/annotation2264357/range2264357/1
type: Range
persistent identity: range2264357
display id: range2264357
version: 1
direction: direction/reverse
top level: BBa_K574009
start: 991
end: 1147
orientation: inline

BBa_K574008
type: SequenceAnnotation
persistent identity: annotation2264352
display id: annotation2264352
version: 1
top level: BBa_K574009
location: https://synbiohub.org/public/igem/BBa_K574009/annotation2264352/range2264352/1
component: BBa_K574008

https://synbiohub.org/public/igem/BBa_K574009/annotation2264352/range2264352/1
type: Range
persistent identity: range2264352
display id: range2264352
version: 1
direction: direction/reverse
top level: BBa_K574009
start: 1
end: 982
orientation: inline

lasR
type: ComponentDefinition
persistent identity: BBa C0179
display id: BBa_C0179
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: lasR activator from P. aeruginosa PAO1(no LVA)
creator: jcbraff
created: 2004-05-26T11:00:00Z
modified: 2015-08-31T04:07:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_1_
m user id: 0
owner id: 61
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: same as C0079 except no LVA tag
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_C0179_sequence/1

BBa_K1631024
type: ComponentDefinition
persistent identity: BBa K1631024
display id: BBa_K1631024
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Colicin-E3 cassette (Strong)
creator: Yuto Yamanaka
created: 2015-09-13T11:00:00Z
modified: 2015-09-18T06:22:43Z
discontinued: false
dominant: false
group u list: _2048_
m user id: 23567
owner id: 23567
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Colicin-E3, its immunity protein, and colicin lysis protein is constitutively expressed strong.(under BBa_?J23101) When this device works, Colicin-E3 will be released to media, and E. coli strains which does not have immunity protein will be killed.
mutable notes: none
mutable provenance: Assembly
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_K1631009
component: BBa_J23101
sequence annotation: BBa_J23101
sequence annotation: BBa_K1631009
sequence: https://synbiohub.org/public/igem/BBa_K1631024_sequence/1

BBa_K1631009
type: Component
persistent identity: component2455792
display id: component2455792
version: 1
top level: BBa_K1631024
access: public
definition: https://synbiohub.org/public/igem/BBa_K1631009/1

BBa_J23101
type: Component
persistent identity: component2455775
display id: component2455775
version: 1
top level: BBa_K1631024
access: public
definition: https://synbiohub.org/public/igem/BBa_J23101/1

BBa_J23101
type: SequenceAnnotation
persistent identity: annotation2455775
display id: annotation2455775
version: 1
top level: BBa_K1631024
location: https://synbiohub.org/public/igem/BBa_K1631024/annotation2455775/range2455775/1
component: BBa_J23101

https://synbiohub.org/public/igem/BBa_K1631024/annotation2455775/range2455775/1
type: Range
persistent identity: range2455775
display id: range2455775
version: 1
direction: direction/reverse
top level: BBa_K1631024
start: 1
end: 35
orientation: inline

BBa_K1631009
type: SequenceAnnotation
persistent identity: annotation2455792
display id: annotation2455792
version: 1
top level: BBa_K1631024
location: https://synbiohub.org/public/igem/BBa_K1631024/annotation2455792/range2455792/1
component: BBa_K1631009

https://synbiohub.org/public/igem/BBa_K1631024/annotation2455792/range2455792/1
type: Range
persistent identity: range2455792
display id: range2455792
version: 1
direction: direction/reverse
top level: BBa_K1631024
start: 44
end: 2290
orientation: inline

BBa_K082020
type: ComponentDefinition
persistent identity: BBa K082020
display id: BBa_K082020
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LuxR generator
creator: Zhen Xu , Binjie Xu
created: 2008-10-28T12:00:00Z
modified: 2015-05-08T01:08:35Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _228_
m user id: 0
owner id: 1560
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: none
mutable notes: none
mutable provenance: none
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_I0462
component: BBa_R0040
sequence annotation: BBa_R0040
sequence annotation: BBa_I0462
sequence: https://synbiohub.org/public/igem/BBa_K082020_sequence/1

BBa_I0462
type: Component
persistent identity: component2254875
display id: component2254875
version: 1
top level: BBa_K082020
access: public
definition: LuxR

BBa_R0040
type: Component
persistent identity: component2254856
display id: component2254856
version: 1
top level: BBa_K082020
access: public
definition: https://synbiohub.org/public/igem/BBa_R0040/1

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2254856
display id: annotation2254856
version: 1
top level: BBa_K082020
location: https://synbiohub.org/public/igem/BBa_K082020/annotation2254856/range2254856/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_K082020/annotation2254856/range2254856/1
type: Range
persistent identity: range2254856
display id: range2254856
version: 1
direction: direction/forward
top level: BBa_K082020
start: 1
end: 54
orientation: inline

BBa_I0462
type: SequenceAnnotation
persistent identity: annotation2254875
display id: annotation2254875
version: 1
top level: BBa_K082020
location: https://synbiohub.org/public/igem/BBa_K082020/annotation2254875/range2254875/1
component: BBa_I0462

https://synbiohub.org/public/igem/BBa_K082020/annotation2254875/range2254875/1
type: Range
persistent identity: range2254875
display id: range2254875
version: 1
direction: direction/reverse
top level: BBa_K082020
start: 63
end: 998
orientation: inline

lasr
type: ComponentDefinition
persistent identity: BBa C0079
display id: BBa_C0079
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: lasR activator from P. aeruginosa PAO1(+LVA)
creator: Alvin Carter Powers (Fighting Darwins)
created: 2004-01-27T12:00:00Z
modified: 2015-08-31T04:07:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: coding region for lasR protein, which accepts chemical signal AI-1 (made by lasI protein)
mutable notes: Mutated 480 from g to a to remove PstI site
mutable provenance: Genbank: NC_002516 (www.ncbi.nlm.nih.gov)
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Help:Barcodes
sequence annotation: G
sequence annotation: lasR
sequence annotation: LVA
sequence: https://synbiohub.org/public/igem/BBa_C0079_sequence/1

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213999
display id: annotation2213999
version: 1
top level: lasr
role: SO:0000807
role: feature/barcode
location: https://synbiohub.org/public/igem/BBa_C0079/annotation2213999/range2213999/1

https://synbiohub.org/public/igem/BBa_C0079/annotation2213999/range2213999/1
type: Range
persistent identity: range2213999
display id: range2213999
version: 1
direction: direction/forward
top level: lasr
start: 757
end: 781
orientation: inline

G
type: SequenceAnnotation
persistent identity: annotation308006
display id: annotation308006
version: 1
top level: lasr
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_C0079/annotation308006/range308006/1

https://synbiohub.org/public/igem/BBa_C0079/annotation308006/range308006/1
type: Range
persistent identity: range308006
display id: range308006
version: 1
direction: direction/forward
top level: lasr
start: 480
end: 480
orientation: inline

lasR
type: SequenceAnnotation
persistent identity: annotation307935
display id: annotation307935
version: 1
top level: lasr
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_C0079/annotation307935/range307935/1

https://synbiohub.org/public/igem/BBa_C0079/annotation307935/range307935/1
type: Range
persistent identity: range307935
display id: range307935
version: 1
direction: direction/forward
top level: lasr
start: 1
end: 717
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation307948
display id: annotation307948
version: 1
top level: lasr
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_C0079/annotation307948/range307948/1

https://synbiohub.org/public/igem/BBa_C0079/annotation307948/range307948/1
type: Range
persistent identity: range307948
display id: range307948
version: 1
direction: direction/forward
top level: lasr
start: 718
end: 750
orientation: inline

BBa_K546002
type: ComponentDefinition
persistent identity: BBa K546002
display id: BBa_K546002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Lux pL controlled luxR with lux pR controlled GFP(lva tag).
creator: M. Hesselman
created: 2011-09-13T11:00:00Z
modified: 2015-05-08T01:12:39Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _713_
m user id: 0
owner id: 9055
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This BioBrick system is able to notify the downstream activation of the lux R promoter of the 3OC6HSL Receiver Device.
mutable notes: The original R0063 BioBrick part (promoter) of the F2621 BioBrick device turned out to be absent in our construct, as was shown by a sequence analysis. To increase the transcription activation there has been put an extra R0063 BioBrick part promoter in front of the F2621 BioBrick device.
mutable provenance: The lux p promoters originate from the quorum sensing ability of ''Vibrio fischeri''.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_F2621
component: BBa_I763020
sequence annotation: BBa_I763020
sequence annotation: BBa_F2621
sequence: https://synbiohub.org/public/igem/BBa_K546002_sequence/1

BBa_F2621
type: Component
persistent identity: component2128274
display id: component2128274
version: 1
top level: BBa_K546002
access: public
definition: BBa_F2621

BBa_I763020
type: Component
persistent identity: component2128288
display id: component2128288
version: 1
top level: BBa_K546002
access: public
definition: https://synbiohub.org/public/igem/BBa_I763020/1

BBa_I763020
type: SequenceAnnotation
persistent identity: annotation2128288
display id: annotation2128288
version: 1
top level: BBa_K546002
location: https://synbiohub.org/public/igem/BBa_K546002/annotation2128288/range2128288/1
component: BBa_I763020

https://synbiohub.org/public/igem/BBa_K546002/annotation2128288/range2128288/1
type: Range
persistent identity: range2128288
display id: range2128288
version: 1
direction: direction/reverse
top level: BBa_K546002
start: 1167
end: 2080
orientation: inline

BBa_F2621
type: SequenceAnnotation
persistent identity: annotation2128274
display id: annotation2128274
version: 1
top level: BBa_K546002
location: https://synbiohub.org/public/igem/BBa_K546002/annotation2128274/range2128274/1
component: BBa_F2621

https://synbiohub.org/public/igem/BBa_K546002/annotation2128274/range2128274/1
type: Range
persistent identity: range2128274
display id: range2128274
version: 1
direction: direction/reverse
top level: BBa_K546002
start: 1
end: 1158
orientation: inline

Rec-Closed
type: ComponentDefinition
persistent identity: BBa I726071
display id: BBa_I726071
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Rec-RRY
creator: Bangalore iGEM
created: 2007-07-03T11:00:00Z
modified: 2015-08-31T04:07:55Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _107_
m user id: 0
owner id: 2077
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: Abcdefghijkl..
mutable notes: none
mutable provenance: Registry
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_C0062
component: BBa_R0062
component: BBa_E0030
component: BBa_B0012
component: BBa_B0010
component: BBa_B0034
sequence annotation: BBa_C0062
sequence annotation: BBa_B0010
sequence annotation: BBa_B0034
sequence annotation: BBa_E0030
sequence annotation: BBa_R0062
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_I726071_sequence/1

BBa_C0062
type: Component
persistent identity: component2265620
display id: component2265620
version: 1
top level: Rec-Closed
access: public
definition: luxr

BBa_R0062
type: Component
persistent identity: component2265611
display id: component2265611
version: 1
top level: Rec-Closed
access: public
definition: https://synbiohub.org/public/igem/BBa_R0062/1

BBa_E0030
type: Component
persistent identity: component2265626
display id: component2265626
version: 1
top level: Rec-Closed
access: public
definition: https://synbiohub.org/public/igem/BBa_E0030/1

BBa_B0012
type: Component
persistent identity: component2265629
display id: component2265629
version: 1
top level: Rec-Closed
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0010
type: Component
persistent identity: component2265627
display id: component2265627
version: 1
top level: Rec-Closed
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0034
type: Component
persistent identity: component2265617
display id: component2265617
version: 1
top level: Rec-Closed
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0034
type: Component
persistent identity: component2265625
display id: component2265625
version: 1
top level: Rec-Closed
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_C0062
type: SequenceAnnotation
persistent identity: annotation2265620
display id: annotation2265620
version: 1
top level: Rec-Closed
location: https://synbiohub.org/public/igem/BBa_I726071/annotation2265620/range2265620/1
component: BBa_C0062

https://synbiohub.org/public/igem/BBa_I726071/annotation2265620/range2265620/1
type: Range
persistent identity: range2265620
display id: range2265620
version: 1
direction: direction/forward
top level: Rec-Closed
start: 82
end: 837
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2265627
display id: annotation2265627
version: 1
top level: Rec-Closed
location: https://synbiohub.org/public/igem/BBa_I726071/annotation2265627/range2265627/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I726071/annotation2265627/range2265627/1
type: Range
persistent identity: range2265627
display id: range2265627
version: 1
direction: direction/forward
top level: Rec-Closed
start: 1620
end: 1699
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2265625
display id: annotation2265625
version: 1
top level: Rec-Closed
location: https://synbiohub.org/public/igem/BBa_I726071/annotation2265625/range2265625/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I726071/annotation2265625/range2265625/1
type: Range
persistent identity: range2265625
display id: range2265625
version: 1
direction: direction/reverse
top level: Rec-Closed
start: 871
end: 882
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2265617
display id: annotation2265617
version: 1
top level: Rec-Closed
location: https://synbiohub.org/public/igem/BBa_I726071/annotation2265617/range2265617/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I726071/annotation2265617/range2265617/1
type: Range
persistent identity: range2265617
display id: range2265617
version: 1
direction: direction/reverse
top level: Rec-Closed
start: 64
end: 75
orientation: inline

BBa_E0030
type: SequenceAnnotation
persistent identity: annotation2265626
display id: annotation2265626
version: 1
top level: Rec-Closed
location: https://synbiohub.org/public/igem/BBa_I726071/annotation2265626/range2265626/1
component: BBa_E0030

https://synbiohub.org/public/igem/BBa_I726071/annotation2265626/range2265626/1
type: Range
persistent identity: range2265626
display id: range2265626
version: 1
direction: direction/reverse
top level: Rec-Closed
start: 889
end: 1611
orientation: inline

BBa_R0062
type: SequenceAnnotation
persistent identity: annotation2265611
display id: annotation2265611
version: 1
top level: Rec-Closed
location: https://synbiohub.org/public/igem/BBa_I726071/annotation2265611/range2265611/1
component: BBa_R0062

https://synbiohub.org/public/igem/BBa_I726071/annotation2265611/range2265611/1
type: Range
persistent identity: range2265611
display id: range2265611
version: 1
direction: direction/forward
top level: Rec-Closed
start: 1
end: 55
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2265629
display id: annotation2265629
version: 1
top level: Rec-Closed
location: https://synbiohub.org/public/igem/BBa_I726071/annotation2265629/range2265629/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I726071/annotation2265629/range2265629/1
type: Range
persistent identity: range2265629
display id: range2265629
version: 1
direction: direction/forward
top level: Rec-Closed
start: 1708
end: 1748
orientation: inline

BBa_J07073
type: ComponentDefinition
persistent identity: BBa J07073
display id: BBa_J07073
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: -- No description --
creator: Will Bosworth
created: 2005-08-22T11:00:00Z
modified: 2015-08-31T04:08:20Z
discontinued: false
dominant: false
group u list: _21_
m user id: 0
owner id: 363
owning group id: 21
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: ToxR' aa's 1-210 transmembrane (inner membrane) dimerizing protein. Used in conjunction with J07007-ctx_promotor for dimerization controlled gene expression. aa's 1-210 (abridged from 1-294) removes the w.t. periplasmic region of the protein with intent to replace region with specific binding molecules.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Signalling
role: SO:0000804
sequence: https://synbiohub.org/public/igem/BBa_J07073_sequence/1

BBa_K1072010
type: ComponentDefinition
persistent identity: BBa K1072010
display id: BBa_K1072010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Odr-10(diacetyl)GPCR with optimized codon
creator: Junqi kuang, Xihao liao and Jianhui gong
created: 2013-09-08T11:00:00Z
modified: 2015-05-08T01:09:01Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1381_
m user id: 0
owner id: 16002
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Odr-10(diacetyl)GPCR encodes a diacetyl-sensing G-protein coupled receptor, originating from the C. elegans. If BBa_K1072010 was transformed into the yeast, the pheromone receptors will be actived and coupled to a heterotrimeric G protein, Odr-10. pathway is composedof Gpa1p (Gα), Ste4p (Gβ) and Ste18p (Gγ) subunits.The Gβγ particle transmits the signal to a MAP kinase module and finally follow a inducible promotor FUS1, which will link a reproter, such as GFP, His3 or LacZ.
mutable notes: As discription of most paper, RNA of Odr-10 be used as plate and transform into receptor cells to express member protein successfully.
mutable provenance: Originate from the C. elegans.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000834
role: partType/RNA
sequence annotation: Odr-10 coding region
sequence: https://synbiohub.org/public/igem/BBa_K1072010_sequence/1

Odr-10 coding region
type: SequenceAnnotation
persistent identity: annotation2357697
display id: annotation2357697
version: 1
top level: BBa_K1072010
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1072010/annotation2357697/range2357697/1

https://synbiohub.org/public/igem/BBa_K1072010/annotation2357697/range2357697/1
type: Range
persistent identity: range2357697
display id: range2357697
version: 1
direction: direction/forward
top level: BBa_K1072010
start: 1
end: 1020
orientation: inline

BBa_K082010
type: ComponentDefinition
persistent identity: BBa K082010
display id: BBa_K082010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: B0034-C0070
creator: Zhen Xu , Binjie Xu
created: 2008-10-27T12:00:00Z
modified: 2015-05-08T01:08:35Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _228_
m user id: 0
owner id: 1560
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: none
mutable notes: none
mutable provenance: none
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_C0070
component: BBa_B0034
sequence annotation: BBa_B0034
sequence annotation: BBa_C0070
sequence: https://synbiohub.org/public/igem/BBa_K082010_sequence/1

BBa_C0070
type: Component
persistent identity: component2244063
display id: component2244063
version: 1
top level: BBa_K082010
access: public
definition: rhII

BBa_B0034
type: Component
persistent identity: component2244059
display id: component2244059
version: 1
top level: BBa_K082010
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2244059
display id: annotation2244059
version: 1
top level: BBa_K082010
location: https://synbiohub.org/public/igem/BBa_K082010/annotation2244059/range2244059/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K082010/annotation2244059/range2244059/1
type: Range
persistent identity: range2244059
display id: range2244059
version: 1
direction: direction/reverse
top level: BBa_K082010
start: 1
end: 12
orientation: inline

BBa_C0070
type: SequenceAnnotation
persistent identity: annotation2244063
display id: annotation2244063
version: 1
top level: BBa_K082010
location: https://synbiohub.org/public/igem/BBa_K082010/annotation2244063/range2244063/1
component: BBa_C0070

https://synbiohub.org/public/igem/BBa_K082010/annotation2244063/range2244063/1
type: Range
persistent identity: range2244063
display id: range2244063
version: 1
direction: direction/reverse
top level: BBa_K082010
start: 19
end: 685
orientation: inline

BBa_I0429
type: ComponentDefinition
persistent identity: BBa I0429
display id: BBa_I0429
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: I0408.I6116
creator: jcbraff
created: 2004-07-13T11:00:00Z
modified: 2015-08-31T04:07:29Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_1_
m user id: 0
owner id: 61
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: I0408.I6116 Cin test
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Signalling
role: SO:0000804
component: BBa_B0012
component: BBa_C0077
component: BBa_B0034
component: BBa_R0077
component: BBa_E0030
component: BBa_B0010
component: BBa_R0040
component: BBa_E0020
sequence annotation: BBa_B0010
sequence annotation: BBa_R0040
sequence annotation: BBa_R0077
sequence annotation: BBa_E0030
sequence annotation: BBa_B0012
sequence annotation: BBa_E0020
sequence annotation: BBa_B0034
sequence annotation: BBa_C0077
sequence: https://synbiohub.org/public/igem/BBa_I0429_sequence/1

BBa_B0012
type: Component
persistent identity: component971656
display id: component971656
version: 1
top level: BBa_I0429
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_C0077
type: Component
persistent identity: component971633
display id: component971633
version: 1
top level: BBa_I0429
access: public
definition: cinr

BBa_B0034
type: Component
persistent identity: component971623
display id: component971623
version: 1
top level: BBa_I0429
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_R0077
type: Component
persistent identity: component971673
display id: component971673
version: 1
top level: BBa_I0429
access: public
definition: https://synbiohub.org/public/igem/BBa_R0077/1

BBa_E0030
type: Component
persistent identity: component971675
display id: component971675
version: 1
top level: BBa_I0429
access: public
definition: https://synbiohub.org/public/igem/BBa_E0030/1

BBa_B0010
type: Component
persistent identity: component971646
display id: component971646
version: 1
top level: BBa_I0429
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_R0040
type: Component
persistent identity: component971615
display id: component971615
version: 1
top level: BBa_I0429
access: public
definition: https://synbiohub.org/public/igem/BBa_R0040/1

BBa_B0034
type: Component
persistent identity: component971639
display id: component971639
version: 1
top level: BBa_I0429
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_E0020
type: Component
persistent identity: component971641
display id: component971641
version: 1
top level: BBa_I0429
access: public
definition: https://synbiohub.org/public/igem/BBa_E0020/1

BBa_B0012
type: Component
persistent identity: component971690
display id: component971690
version: 1
top level: BBa_I0429
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0010
type: Component
persistent identity: component971680
display id: component971680
version: 1
top level: BBa_I0429
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation971646
display id: annotation971646
version: 1
top level: BBa_I0429
location: https://synbiohub.org/public/igem/BBa_I0429/annotation971646/range971646/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I0429/annotation971646/range971646/1
type: Range
persistent identity: range971646
display id: range971646
version: 1
direction: direction/forward
top level: BBa_I0429
start: 1625
end: 1704
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation971615
display id: annotation971615
version: 1
top level: BBa_I0429
location: https://synbiohub.org/public/igem/BBa_I0429/annotation971615/range971615/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_I0429/annotation971615/range971615/1
type: Range
persistent identity: range971615
display id: range971615
version: 1
direction: direction/forward
top level: BBa_I0429
start: 1
end: 54
orientation: inline

BBa_R0077
type: SequenceAnnotation
persistent identity: annotation971673
display id: annotation971673
version: 1
top level: BBa_I0429
location: https://synbiohub.org/public/igem/BBa_I0429/annotation971673/range971673/1
component: BBa_R0077

https://synbiohub.org/public/igem/BBa_I0429/annotation971673/range971673/1
type: Range
persistent identity: range971673
display id: range971673
version: 1
direction: direction/forward
top level: BBa_I0429
start: 1762
end: 1992
orientation: inline

BBa_E0030
type: SequenceAnnotation
persistent identity: annotation971675
display id: annotation971675
version: 1
top level: BBa_I0429
location: https://synbiohub.org/public/igem/BBa_I0429/annotation971675/range971675/1
component: BBa_E0030

https://synbiohub.org/public/igem/BBa_I0429/annotation971675/range971675/1
type: Range
persistent identity: range971675
display id: range971675
version: 1
direction: direction/forward
top level: BBa_I0429
start: 1999
end: 2721
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation971680
display id: annotation971680
version: 1
top level: BBa_I0429
location: https://synbiohub.org/public/igem/BBa_I0429/annotation971680/range971680/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I0429/annotation971680/range971680/1
type: Range
persistent identity: range971680
display id: range971680
version: 1
direction: direction/forward
top level: BBa_I0429
start: 2730
end: 2809
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation971690
display id: annotation971690
version: 1
top level: BBa_I0429
location: https://synbiohub.org/public/igem/BBa_I0429/annotation971690/range971690/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I0429/annotation971690/range971690/1
type: Range
persistent identity: range971690
display id: range971690
version: 1
direction: direction/forward
top level: BBa_I0429
start: 2818
end: 2858
orientation: inline

BBa_E0020
type: SequenceAnnotation
persistent identity: annotation971641
display id: annotation971641
version: 1
top level: BBa_I0429
location: https://synbiohub.org/public/igem/BBa_I0429/annotation971641/range971641/1
component: BBa_E0020

https://synbiohub.org/public/igem/BBa_I0429/annotation971641/range971641/1
type: Range
persistent identity: range971641
display id: range971641
version: 1
direction: direction/forward
top level: BBa_I0429
start: 894
end: 1616
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation971639
display id: annotation971639
version: 1
top level: BBa_I0429
location: https://synbiohub.org/public/igem/BBa_I0429/annotation971639/range971639/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I0429/annotation971639/range971639/1
type: Range
persistent identity: range971639
display id: range971639
version: 1
direction: direction/forward
top level: BBa_I0429
start: 876
end: 887
orientation: inline

BBa_C0077
type: SequenceAnnotation
persistent identity: annotation971633
display id: annotation971633
version: 1
top level: BBa_I0429
location: https://synbiohub.org/public/igem/BBa_I0429/annotation971633/range971633/1
component: BBa_C0077

https://synbiohub.org/public/igem/BBa_I0429/annotation971633/range971633/1
type: Range
persistent identity: range971633
display id: range971633
version: 1
direction: direction/forward
top level: BBa_I0429
start: 81
end: 842
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation971623
display id: annotation971623
version: 1
top level: BBa_I0429
location: https://synbiohub.org/public/igem/BBa_I0429/annotation971623/range971623/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I0429/annotation971623/range971623/1
type: Range
persistent identity: range971623
display id: range971623
version: 1
direction: direction/forward
top level: BBa_I0429
start: 63
end: 74
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation971656
display id: annotation971656
version: 1
top level: BBa_I0429
location: https://synbiohub.org/public/igem/BBa_I0429/annotation971656/range971656/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I0429/annotation971656/range971656/1
type: Range
persistent identity: range971656
display id: range971656
version: 1
direction: direction/forward
top level: BBa_I0429
start: 1713
end: 1753
orientation: inline

BBa_K082007
type: ComponentDefinition
persistent identity: BBa K082007
display id: BBa_K082007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: B0030-C0070
creator: Zhen Xu , Binjie Xu
created: 2008-10-27T12:00:00Z
modified: 2015-05-08T01:08:35Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _228_
m user id: 0
owner id: 1560
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: none
mutable notes: none
mutable provenance: none
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_B0030
component: BBa_C0070
sequence annotation: BBa_B0030
sequence annotation: BBa_C0070
sequence: https://synbiohub.org/public/igem/BBa_K082007_sequence/1

BBa_B0030
type: Component
persistent identity: component2244039
display id: component2244039
version: 1
top level: BBa_K082007
access: public
definition: https://synbiohub.org/public/igem/BBa_B0030/1

BBa_C0070
type: Component
persistent identity: component2244044
display id: component2244044
version: 1
top level: BBa_K082007
access: public
definition: rhII

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation2244039
display id: annotation2244039
version: 1
top level: BBa_K082007
location: https://synbiohub.org/public/igem/BBa_K082007/annotation2244039/range2244039/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_K082007/annotation2244039/range2244039/1
type: Range
persistent identity: range2244039
display id: range2244039
version: 1
direction: direction/forward
top level: BBa_K082007
start: 1
end: 15
orientation: inline

BBa_C0070
type: SequenceAnnotation
persistent identity: annotation2244044
display id: annotation2244044
version: 1
top level: BBa_K082007
location: https://synbiohub.org/public/igem/BBa_K082007/annotation2244044/range2244044/1
component: BBa_C0070

https://synbiohub.org/public/igem/BBa_K082007/annotation2244044/range2244044/1
type: Range
persistent identity: range2244044
display id: range2244044
version: 1
direction: direction/reverse
top level: BBa_K082007
start: 22
end: 688
orientation: inline

LuxOU
type: ComponentDefinition
persistent identity: BBa K131016
display id: BBa_K131016
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LuxOU from Vibrio harveyi
creator: Thane Kubik
created: 2008-09-27T11:00:00Z
modified: 2015-05-08T01:09:47Z
discontinued: false
dominant: true
experience: experience/None
group u list: _259_
m user id: 0
owner id: 3190
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: Vibrio harveyi repressor protein LuxO and phosphorelay protein LuxU genes. LuxO and LuxU are regulators of luminescence in Vibrio harveyi.
mutable notes: LuxO and LuxU are found within the operon, therefore we have decided to keep these genes together. The natural RBS was also kept.
mutable provenance: GenBank Accession# L26221 LuxO Protein ID# AAD12736.1 LuxU Protein ID# AAD12737.2 Jeremy A. Freeman and Bonnie L. Bassler. Sequence and function of LuxU: a two-component phosphorelay protein that regulates quorum sensing in Vibrio harveyi. 1999. Journal of Bacteriology. Feb 1999, p. 899-906.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
sequence annotation: Stop for LuxU
sequence annotation: Start for LuxU
sequence annotation: LuxU
sequence annotation: RBS
sequence annotation: LuxO
sequence annotation: Stop for LuxO
sequence annotation: Start for LuxO
sequence: https://synbiohub.org/public/igem/BBa_K131016_sequence/1

Stop for LuxU
type: SequenceAnnotation
persistent identity: annotation1976861
display id: annotation1976861
version: 1
top level: LuxOU
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K131016/annotation1976861/range1976861/1

https://synbiohub.org/public/igem/BBa_K131016/annotation1976861/range1976861/1
type: Range
persistent identity: range1976861
display id: range1976861
version: 1
direction: direction/forward
top level: LuxOU
start: 1731
end: 1733
orientation: inline

Start for LuxU
type: SequenceAnnotation
persistent identity: annotation1976860
display id: annotation1976860
version: 1
top level: LuxOU
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K131016/annotation1976860/range1976860/1

https://synbiohub.org/public/igem/BBa_K131016/annotation1976860/range1976860/1
type: Range
persistent identity: range1976860
display id: range1976860
version: 1
direction: direction/forward
top level: LuxOU
start: 1389
end: 1391
orientation: inline

LuxU
type: SequenceAnnotation
persistent identity: annotation1976859
display id: annotation1976859
version: 1
top level: LuxOU
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K131016/annotation1976859/range1976859/1

https://synbiohub.org/public/igem/BBa_K131016/annotation1976859/range1976859/1
type: Range
persistent identity: range1976859
display id: range1976859
version: 1
direction: direction/forward
top level: LuxOU
start: 1389
end: 1733
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1976855
display id: annotation1976855
version: 1
top level: LuxOU
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K131016/annotation1976855/range1976855/1

https://synbiohub.org/public/igem/BBa_K131016/annotation1976855/range1976855/1
type: Range
persistent identity: range1976855
display id: range1976855
version: 1
direction: direction/forward
top level: LuxOU
start: 5
end: 12
orientation: inline

LuxO
type: SequenceAnnotation
persistent identity: annotation1976856
display id: annotation1976856
version: 1
top level: LuxOU
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K131016/annotation1976856/range1976856/1

https://synbiohub.org/public/igem/BBa_K131016/annotation1976856/range1976856/1
type: Range
persistent identity: range1976856
display id: range1976856
version: 1
direction: direction/forward
top level: LuxOU
start: 31
end: 1392
orientation: inline

Stop for LuxO
type: SequenceAnnotation
persistent identity: annotation1976858
display id: annotation1976858
version: 1
top level: LuxOU
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K131016/annotation1976858/range1976858/1

https://synbiohub.org/public/igem/BBa_K131016/annotation1976858/range1976858/1
type: Range
persistent identity: range1976858
display id: range1976858
version: 1
direction: direction/forward
top level: LuxOU
start: 1390
end: 1392
orientation: inline

Start for LuxO
type: SequenceAnnotation
persistent identity: annotation1976857
display id: annotation1976857
version: 1
top level: LuxOU
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K131016/annotation1976857/range1976857/1

https://synbiohub.org/public/igem/BBa_K131016/annotation1976857/range1976857/1
type: Range
persistent identity: range1976857
display id: range1976857
version: 1
direction: direction/forward
top level: LuxOU
start: 31
end: 33
orientation: inline

LuxO D47A
type: ComponentDefinition
persistent identity: BBa K131023
display id: BBa_K131023
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LuxO D47A, Vibrio harveyi
creator: Thane Kubik
created: 2008-10-27T12:00:00Z
modified: 2015-05-08T01:09:47Z
discontinued: false
dominant: true
experience: experience/None
group u list: _259_
m user id: 0
owner id: 3190
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: LuxO D47A is a inactive mutant of LuxO.
mutable notes: Plasmid containing LuxO D47A (pJAF544) was obtained from Bassler lab (Freeman and Bassler, 1999).
mutable provenance: Jeremy A. Freeman and Bonnie L. Bassler. A genetic analysis of the function of LuxO, a two-component response regulator involved in quorum sensing in Vibrio harveyi. 1999a. Molecular Microbiology. 31(2), 665-677.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Stop
sequence annotation: Start
sequence annotation: D47A
sequence: https://synbiohub.org/public/igem/BBa_K131023_sequence/1

Stop
type: SequenceAnnotation
persistent identity: annotation1990659
display id: annotation1990659
version: 1
top level: LuxO D47A
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K131023/annotation1990659/range1990659/1

https://synbiohub.org/public/igem/BBa_K131023/annotation1990659/range1990659/1
type: Range
persistent identity: range1990659
display id: range1990659
version: 1
direction: direction/forward
top level: LuxO D47A
start: 1360
end: 1362
orientation: inline

Start
type: SequenceAnnotation
persistent identity: annotation1990658
display id: annotation1990658
version: 1
top level: LuxO D47A
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K131023/annotation1990658/range1990658/1

https://synbiohub.org/public/igem/BBa_K131023/annotation1990658/range1990658/1
type: Range
persistent identity: range1990658
display id: range1990658
version: 1
direction: direction/forward
top level: LuxO D47A
start: 1
end: 3
orientation: inline

D47A
type: SequenceAnnotation
persistent identity: annotation1990657
display id: annotation1990657
version: 1
top level: LuxO D47A
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K131023/annotation1990657/range1990657/1

https://synbiohub.org/public/igem/BBa_K131023/annotation1990657/range1990657/1
type: Range
persistent identity: range1990657
display id: range1990657
version: 1
direction: direction/forward
top level: LuxO D47A
start: 139
end: 141
orientation: inline

BBa_I0468
type: ComponentDefinition
persistent identity: BBa I0468
display id: BBa_I0468
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Cin R Protein Generator
creator: mit
created: 2004-07-12T11:00:00Z
modified: 2015-08-31T04:07:29Z
discontinued: false
dominant: true
experience: experience/None
group u list: _6_
m user id: 0
owner id: 101
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: The Cin R (LVA+) protein can be used to sense 3OC14HSL in the media as part of a receiver device
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Signalling
role: SO:0000804
component: BBa_B0010
component: BBa_B0012
component: BBa_B0034
component: BBa_C0077
sequence annotation: BBa_C0077
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_I0468_sequence/1

BBa_B0010
type: Component
persistent identity: component943384
display id: component943384
version: 1
top level: BBa_I0468
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
type: Component
persistent identity: component943394
display id: component943394
version: 1
top level: BBa_I0468
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0034
type: Component
persistent identity: component943368
display id: component943368
version: 1
top level: BBa_I0468
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_C0077
type: Component
persistent identity: component943378
display id: component943378
version: 1
top level: BBa_I0468
access: public
definition: cinr

BBa_C0077
type: SequenceAnnotation
persistent identity: annotation943378
display id: annotation943378
version: 1
top level: BBa_I0468
location: https://synbiohub.org/public/igem/BBa_I0468/annotation943378/range943378/1
component: BBa_C0077

https://synbiohub.org/public/igem/BBa_I0468/annotation943378/range943378/1
type: Range
persistent identity: range943378
display id: range943378
version: 1
direction: direction/forward
top level: BBa_I0468
start: 19
end: 780
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation943384
display id: annotation943384
version: 1
top level: BBa_I0468
location: https://synbiohub.org/public/igem/BBa_I0468/annotation943384/range943384/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I0468/annotation943384/range943384/1
type: Range
persistent identity: range943384
display id: range943384
version: 1
direction: direction/forward
top level: BBa_I0468
start: 814
end: 893
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation943394
display id: annotation943394
version: 1
top level: BBa_I0468
location: https://synbiohub.org/public/igem/BBa_I0468/annotation943394/range943394/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I0468/annotation943394/range943394/1
type: Range
persistent identity: range943394
display id: range943394
version: 1
direction: direction/forward
top level: BBa_I0468
start: 902
end: 942
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation943368
display id: annotation943368
version: 1
top level: BBa_I0468
location: https://synbiohub.org/public/igem/BBa_I0468/annotation943368/range943368/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I0468/annotation943368/range943368/1
type: Range
persistent identity: range943368
display id: range943368
version: 1
direction: direction/forward
top level: BBa_I0468
start: 1
end: 12
orientation: inline

BBa_K1520510
type: ComponentDefinition
persistent identity: BBa K1520510
display id: BBa_K1520510
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PgolTS-golS-PgolB-rbs-luxI-Ter-Pcons2-rbs-luxR-Ter-Prlux-rbs-OMPA-golB-rbs-luxI-Ter
creator: yue wu
created: 2014-09-22T11:00:00Z
modified: 2015-05-08T01:10:49Z
discontinued: false
dominant: false
group u list: _1902_
m user id: 0
owner id: 21318
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: PgolTS-golS-PgolB-rbs-luxI-Ter-Pcons2-rbs-luxR-Ter-Prlux-rbs-OMPA-golB-rbs-luxI-Ter It is a test of our positive feedback circuit for gold ions.
mutable notes: None
mutable provenance: PgolTS-golS-PgolB, luxI, Pcons2, luxR, Prlux, OMPA-golB, Ter
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1520507
component: BBa_K1520003
component: BBa_K1520506
component: BBa_K1520504
component: BBa_R0062
component: BBa_K1520029
sequence annotation: BBa_R0062
sequence annotation: BBa_K1520506
sequence annotation: BBa_K1520003
sequence annotation: BBa_K1520507
sequence annotation: BBa_K1520029
sequence annotation: BBa_K1520504
sequence: https://synbiohub.org/public/igem/BBa_K1520510_sequence/1

BBa_K1520507
type: Component
persistent identity: component2385585
display id: component2385585
version: 1
top level: BBa_K1520510
access: public
definition: https://synbiohub.org/public/igem/BBa_K1520507/1

BBa_K1520003
type: Component
persistent identity: component2385573
display id: component2385573
version: 1
top level: BBa_K1520510
access: public
definition: BBa_K1520003

BBa_K1520506
type: Component
persistent identity: component2385563
display id: component2385563
version: 1
top level: BBa_K1520510
access: public
definition: https://synbiohub.org/public/igem/BBa_K1520506/1

BBa_K1520504
type: Component
persistent identity: component2385566
display id: component2385566
version: 1
top level: BBa_K1520510
access: public
definition: BBa_K1520504

BBa_K1520504
type: Component
persistent identity: component2385588
display id: component2385588
version: 1
top level: BBa_K1520510
access: public
definition: BBa_K1520504

BBa_R0062
type: Component
persistent identity: component2385575
display id: component2385575
version: 1
top level: BBa_K1520510
access: public
definition: https://synbiohub.org/public/igem/BBa_R0062/1

BBa_K1520029
type: Component
persistent identity: component2385589
display id: component2385589
version: 1
top level: BBa_K1520510
access: public
definition: https://synbiohub.org/public/igem/BBa_K1520029/1

BBa_K1520029
type: Component
persistent identity: component2385567
display id: component2385567
version: 1
top level: BBa_K1520510
access: public
definition: https://synbiohub.org/public/igem/BBa_K1520029/1

BBa_R0062
type: SequenceAnnotation
persistent identity: annotation2385575
display id: annotation2385575
version: 1
top level: BBa_K1520510
location: https://synbiohub.org/public/igem/BBa_K1520510/annotation2385575/range2385575/1
component: BBa_R0062

https://synbiohub.org/public/igem/BBa_K1520510/annotation2385575/range2385575/1
type: Range
persistent identity: range2385575
display id: range2385575
version: 1
direction: direction/forward
top level: BBa_K1520510
start: 2555
end: 2609
orientation: inline

BBa_K1520506
type: SequenceAnnotation
persistent identity: annotation2385563
display id: annotation2385563
version: 1
top level: BBa_K1520510
location: https://synbiohub.org/public/igem/BBa_K1520510/annotation2385563/range2385563/1
component: BBa_K1520506

https://synbiohub.org/public/igem/BBa_K1520510/annotation2385563/range2385563/1
type: Range
persistent identity: range2385563
display id: range2385563
version: 1
direction: direction/reverse
top level: BBa_K1520510
start: 1
end: 753
orientation: inline

BBa_K1520003
type: SequenceAnnotation
persistent identity: annotation2385573
display id: annotation2385573
version: 1
top level: BBa_K1520510
location: https://synbiohub.org/public/igem/BBa_K1520510/annotation2385573/range2385573/1
component: BBa_K1520003

https://synbiohub.org/public/igem/BBa_K1520510/annotation2385573/range2385573/1
type: Range
persistent identity: range2385573
display id: range2385573
version: 1
direction: direction/reverse
top level: BBa_K1520510
start: 1568
end: 2546
orientation: inline

BBa_K1520507
type: SequenceAnnotation
persistent identity: annotation2385585
display id: annotation2385585
version: 1
top level: BBa_K1520510
location: https://synbiohub.org/public/igem/BBa_K1520510/annotation2385585/range2385585/1
component: BBa_K1520507

https://synbiohub.org/public/igem/BBa_K1520510/annotation2385585/range2385585/1
type: Range
persistent identity: range2385585
display id: range2385585
version: 1
direction: direction/reverse
top level: BBa_K1520510
start: 2616
end: 3425
orientation: inline

BBa_K1520029
type: SequenceAnnotation
persistent identity: annotation2385589
display id: annotation2385589
version: 1
top level: BBa_K1520510
location: https://synbiohub.org/public/igem/BBa_K1520510/annotation2385589/range2385589/1
component: BBa_K1520029

https://synbiohub.org/public/igem/BBa_K1520510/annotation2385589/range2385589/1
type: Range
persistent identity: range2385589
display id: range2385589
version: 1
direction: direction/reverse
top level: BBa_K1520510
start: 4103
end: 4231
orientation: inline

BBa_K1520504
type: SequenceAnnotation
persistent identity: annotation2385588
display id: annotation2385588
version: 1
top level: BBa_K1520510
location: https://synbiohub.org/public/igem/BBa_K1520510/annotation2385588/range2385588/1
component: BBa_K1520504

https://synbiohub.org/public/igem/BBa_K1520510/annotation2385588/range2385588/1
type: Range
persistent identity: range2385588
display id: range2385588
version: 1
direction: direction/reverse
top level: BBa_K1520510
start: 3434
end: 4094
orientation: inline

BBa_K1520504
type: SequenceAnnotation
persistent identity: annotation2385566
display id: annotation2385566
version: 1
top level: BBa_K1520510
location: https://synbiohub.org/public/igem/BBa_K1520510/annotation2385566/range2385566/1
component: BBa_K1520504

https://synbiohub.org/public/igem/BBa_K1520510/annotation2385566/range2385566/1
type: Range
persistent identity: range2385566
display id: range2385566
version: 1
direction: direction/reverse
top level: BBa_K1520510
start: 762
end: 1422
orientation: inline

BBa_K1520029
type: SequenceAnnotation
persistent identity: annotation2385567
display id: annotation2385567
version: 1
top level: BBa_K1520510
location: https://synbiohub.org/public/igem/BBa_K1520510/annotation2385567/range2385567/1
component: BBa_K1520029

https://synbiohub.org/public/igem/BBa_K1520510/annotation2385567/range2385567/1
type: Range
persistent identity: range2385567
display id: range2385567
version: 1
direction: direction/reverse
top level: BBa_K1520510
start: 1431
end: 1559
orientation: inline

BBa_I13212
type: ComponentDefinition
persistent identity: BBa I13212
display id: BBa_I13212
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LuxR Protein Generator Controlled by the Left Lux Promoter
creator: Barry Canton
created: 2004-08-04T11:00:00Z
modified: 2015-08-31T04:07:33Z
discontinued: false
dominant: true
experience: experience/None
group u list: _11_6_
m user id: 0
owner id: 135
owning group id: 7
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This is the BioBrick version of the natural LuxR expression device.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Signalling
role: SO:0000804
component: BBa_B0010
component: BBa_R0063
component: BBa_B0012
component: BBa_C0062
component: BBa_B0034
sequence annotation: BBa_C0062
sequence annotation: BBa_B0010
sequence annotation: BBa_R0063
sequence annotation: BBa_B0012
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_I13212_sequence/1

BBa_B0010
type: Component
persistent identity: component1030506
display id: component1030506
version: 1
top level: BBa_I13212
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_R0063
type: Component
persistent identity: component1030474
display id: component1030474
version: 1
top level: BBa_I13212
access: public
definition: https://synbiohub.org/public/igem/BBa_R0063/1

BBa_B0012
type: Component
persistent identity: component1030516
display id: component1030516
version: 1
top level: BBa_I13212
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_C0062
type: Component
persistent identity: component1030498
display id: component1030498
version: 1
top level: BBa_I13212
access: public
definition: luxr

BBa_B0034
type: Component
persistent identity: component1030483
display id: component1030483
version: 1
top level: BBa_I13212
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_C0062
type: SequenceAnnotation
persistent identity: annotation1030498
display id: annotation1030498
version: 1
top level: BBa_I13212
location: https://synbiohub.org/public/igem/BBa_I13212/annotation1030498/range1030498/1
component: BBa_C0062

https://synbiohub.org/public/igem/BBa_I13212/annotation1030498/range1030498/1
type: Range
persistent identity: range1030498
display id: range1030498
version: 1
direction: direction/forward
top level: BBa_I13212
start: 178
end: 933
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1030506
display id: annotation1030506
version: 1
top level: BBa_I13212
location: https://synbiohub.org/public/igem/BBa_I13212/annotation1030506/range1030506/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I13212/annotation1030506/range1030506/1
type: Range
persistent identity: range1030506
display id: range1030506
version: 1
direction: direction/forward
top level: BBa_I13212
start: 967
end: 1046
orientation: inline

BBa_R0063
type: SequenceAnnotation
persistent identity: annotation1030474
display id: annotation1030474
version: 1
top level: BBa_I13212
location: https://synbiohub.org/public/igem/BBa_I13212/annotation1030474/range1030474/1
component: BBa_R0063

https://synbiohub.org/public/igem/BBa_I13212/annotation1030474/range1030474/1
type: Range
persistent identity: range1030474
display id: range1030474
version: 1
direction: direction/forward
top level: BBa_I13212
start: 1
end: 151
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1030516
display id: annotation1030516
version: 1
top level: BBa_I13212
location: https://synbiohub.org/public/igem/BBa_I13212/annotation1030516/range1030516/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I13212/annotation1030516/range1030516/1
type: Range
persistent identity: range1030516
display id: range1030516
version: 1
direction: direction/forward
top level: BBa_I13212
start: 1055
end: 1095
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1030483
display id: annotation1030483
version: 1
top level: BBa_I13212
location: https://synbiohub.org/public/igem/BBa_I13212/annotation1030483/range1030483/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I13212/annotation1030483/range1030483/1
type: Range
persistent identity: range1030483
display id: range1030483
version: 1
direction: direction/forward
top level: BBa_I13212
start: 160
end: 171
orientation: inline

BBa_S04301
type: ComponentDefinition
persistent identity: BBa S04301
display id: BBa_S04301
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: C0079:B0015
creator: UNAM FCiencias-CINVESTAV IRA
created: 2009-10-11T11:00:00Z
modified: 2015-05-08T01:14:38Z
discontinued: false
dominant: false
experience: experience/None
group u list: _9_
m user id: 0
owner id: 1955
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_C0079
component: BBa_B0015
sequence annotation: BBa_B0015
sequence annotation: BBa_C0079
sequence: https://synbiohub.org/public/igem/BBa_S04301_sequence/1

BBa_C0079
type: Component
persistent identity: component2246626
display id: component2246626
version: 1
top level: BBa_S04301
access: public
definition: lasr

BBa_B0015
type: Component
persistent identity: component2246633
display id: component2246633
version: 1
top level: BBa_S04301
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2246633
display id: annotation2246633
version: 1
top level: BBa_S04301
location: https://synbiohub.org/public/igem/BBa_S04301/annotation2246633/range2246633/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_S04301/annotation2246633/range2246633/1
type: Range
persistent identity: range2246633
display id: range2246633
version: 1
direction: direction/reverse
top level: BBa_S04301
start: 790
end: 918
orientation: inline

BBa_C0079
type: SequenceAnnotation
persistent identity: annotation2246626
display id: annotation2246626
version: 1
top level: BBa_S04301
location: https://synbiohub.org/public/igem/BBa_S04301/annotation2246626/range2246626/1
component: BBa_C0079

https://synbiohub.org/public/igem/BBa_S04301/annotation2246626/range2246626/1
type: Range
persistent identity: range2246626
display id: range2246626
version: 1
direction: direction/reverse
top level: BBa_S04301
start: 1
end: 781
orientation: inline

cinr
type: ComponentDefinition
persistent identity: BBa C0077
display id: BBa_C0077
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: cinR activator from Rhizobium leguminosarum (+LVA)
creator: crackdots
created: 2004-01-27T12:00:00Z
modified: 2015-08-31T04:07:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: In complex with O3-C14:1-HSL (made by BBa_C0076), CinR binds to the Cin promoter (BBa_R0077) and activates transcription.
mutable notes: The regulatory locus cinRI in Rhizobium leguminosarum conrols a network of quorum-sensing loci Lithgow, JK; Wilkinson, A; Hardman, A; Rodelas, B; Wisniewski-Dye, F; Williams, P; Downie, AJ MOL. MICROBIOLOGY 37(1): 81-97, 2000 Change log: original STOP: tag -> taATAA
mutable provenance: Rhizobium leguminosarum
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: cinR
sequence annotation: LVA
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0077_sequence/1

cinR
type: SequenceAnnotation
persistent identity: annotation301112
display id: annotation301112
version: 1
top level: cinr
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_C0077/annotation301112/range301112/1

https://synbiohub.org/public/igem/BBa_C0077/annotation301112/range301112/1
type: Range
persistent identity: range301112
display id: range301112
version: 1
direction: direction/forward
top level: cinr
start: 1
end: 723
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation306600
display id: annotation306600
version: 1
top level: cinr
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_C0077/annotation306600/range306600/1

https://synbiohub.org/public/igem/BBa_C0077/annotation306600/range306600/1
type: Range
persistent identity: range306600
display id: range306600
version: 1
direction: direction/forward
top level: cinr
start: 724
end: 756
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2214000
display id: annotation2214000
version: 1
top level: cinr
role: SO:0000807
role: feature/barcode
location: https://synbiohub.org/public/igem/BBa_C0077/annotation2214000/range2214000/1

https://synbiohub.org/public/igem/BBa_C0077/annotation2214000/range2214000/1
type: Range
persistent identity: range2214000
display id: range2214000
version: 1
direction: direction/forward
top level: cinr
start: 763
end: 787
orientation: inline

BBa_K1520501
type: ComponentDefinition
persistent identity: BBa K1520501
display id: BBa_K1520501
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Rbs-luxR(scarless)
creator: yue wu
created: 2014-09-21T11:00:00Z
modified: 2015-05-08T01:10:49Z
discontinued: false
dominant: false
group u list: _1902_
m user id: 0
owner id: 21318
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Rbs-luxR(scarless)
mutable notes: None
mutable provenance: None
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
sequence annotation: rbs
sequence annotation: luxR
sequence: https://synbiohub.org/public/igem/BBa_K1520501_sequence/1

rbs
type: SequenceAnnotation
persistent identity: annotation2384861
display id: annotation2384861
version: 1
top level: BBa_K1520501
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1520501/annotation2384861/range2384861/1

https://synbiohub.org/public/igem/BBa_K1520501/annotation2384861/range2384861/1
type: Range
persistent identity: range2384861
display id: range2384861
version: 1
direction: direction/forward
top level: BBa_K1520501
start: 1
end: 18
orientation: inline

luxR
type: SequenceAnnotation
persistent identity: annotation2384862
display id: annotation2384862
version: 1
top level: BBa_K1520501
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1520501/annotation2384862/range2384862/1

https://synbiohub.org/public/igem/BBa_K1520501/annotation2384862/range2384862/1
type: Range
persistent identity: range2384862
display id: range2384862
version: 1
direction: direction/forward
top level: BBa_K1520501
start: 19
end: 799
orientation: inline

BBa_I724000
type: ComponentDefinition
persistent identity: BBa I724000
display id: BBa_I724000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Autoinducer inactivation enzyme regulated by LuxR/HSL promoter
creator: Hanmo Li
created: 2007-10-25T11:00:00Z
modified: 2015-08-31T04:07:54Z
discontinued: false
dominant: false
group u list: _131_
m user id: 0
owner id: 1540
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: We made this construct with LuxR/HSL promoter R0062, B0034 RBS, and the C0060 aiiA gene in order to give us more control with our repressilator system. The aiiA gene will be turned on by the LuxR/HSL promoter in the presence of the LuxR activator protein complexed with the autoinducer 3-oxo-hexanoyl-HSL.
mutable notes: Standard parts assembly via ligation
mutable provenance: Standard parts from MIT biobricks
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_B0034
component: BBa_C0060
component: BBa_R0062
sequence annotation: BBa_R0062
sequence annotation: BBa_B0034
sequence annotation: BBa_C0060
sequence: https://synbiohub.org/public/igem/BBa_I724000_sequence/1

BBa_B0034
type: Component
persistent identity: component2245755
display id: component2245755
version: 1
top level: BBa_I724000
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_C0060
type: Component
persistent identity: component2245758
display id: component2245758
version: 1
top level: BBa_I724000
access: public
definition: aiiA

BBa_R0062
type: Component
persistent identity: component2245749
display id: component2245749
version: 1
top level: BBa_I724000
access: public
definition: https://synbiohub.org/public/igem/BBa_R0062/1

BBa_R0062
type: SequenceAnnotation
persistent identity: annotation2245749
display id: annotation2245749
version: 1
top level: BBa_I724000
location: https://synbiohub.org/public/igem/BBa_I724000/annotation2245749/range2245749/1
component: BBa_R0062

https://synbiohub.org/public/igem/BBa_I724000/annotation2245749/range2245749/1
type: Range
persistent identity: range2245749
display id: range2245749
version: 1
direction: direction/forward
top level: BBa_I724000
start: 1
end: 55
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2245755
display id: annotation2245755
version: 1
top level: BBa_I724000
location: https://synbiohub.org/public/igem/BBa_I724000/annotation2245755/range2245755/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I724000/annotation2245755/range2245755/1
type: Range
persistent identity: range2245755
display id: range2245755
version: 1
direction: direction/reverse
top level: BBa_I724000
start: 64
end: 75
orientation: inline

BBa_C0060
type: SequenceAnnotation
persistent identity: annotation2245758
display id: annotation2245758
version: 1
top level: BBa_I724000
location: https://synbiohub.org/public/igem/BBa_I724000/annotation2245758/range2245758/1
component: BBa_C0060

https://synbiohub.org/public/igem/BBa_I724000/annotation2245758/range2245758/1
type: Range
persistent identity: range2245758
display id: range2245758
version: 1
direction: direction/forward
top level: BBa_I724000
start: 82
end: 870
orientation: inline

BBa_I0466
type: ComponentDefinition
persistent identity: BBa I0466
display id: BBa_I0466
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RhlR Protein Generator
creator: mit
created: 2004-07-12T11:00:00Z
modified: 2015-08-31T04:07:29Z
discontinued: false
dominant: false
experience: experience/None
group u list: _6_
m user id: 0
owner id: 101
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: RhlR can sense N-butyryl-HSL in the media and can activate transcription from R0071. It can be used as part of a receiver device.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Signalling
role: SO:0000804
component: BBa_B0010
component: BBa_C0071
component: BBa_B0012
component: BBa_B0034
sequence annotation: BBa_B0010
sequence annotation: BBa_B0034
sequence annotation: BBa_B0012
sequence annotation: BBa_C0071
sequence: https://synbiohub.org/public/igem/BBa_I0466_sequence/1

BBa_B0010
type: Component
persistent identity: component943320
display id: component943320
version: 1
top level: BBa_I0466
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_C0071
type: Component
persistent identity: component943314
display id: component943314
version: 1
top level: BBa_I0466
access: public
definition: rhlR

BBa_B0012
type: Component
persistent identity: component943330
display id: component943330
version: 1
top level: BBa_I0466
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0034
type: Component
persistent identity: component943304
display id: component943304
version: 1
top level: BBa_I0466
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation943320
display id: annotation943320
version: 1
top level: BBa_I0466
location: https://synbiohub.org/public/igem/BBa_I0466/annotation943320/range943320/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I0466/annotation943320/range943320/1
type: Range
persistent identity: range943320
display id: range943320
version: 1
direction: direction/forward
top level: BBa_I0466
start: 814
end: 893
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation943304
display id: annotation943304
version: 1
top level: BBa_I0466
location: https://synbiohub.org/public/igem/BBa_I0466/annotation943304/range943304/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I0466/annotation943304/range943304/1
type: Range
persistent identity: range943304
display id: range943304
version: 1
direction: direction/forward
top level: BBa_I0466
start: 1
end: 12
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation943330
display id: annotation943330
version: 1
top level: BBa_I0466
location: https://synbiohub.org/public/igem/BBa_I0466/annotation943330/range943330/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I0466/annotation943330/range943330/1
type: Range
persistent identity: range943330
display id: range943330
version: 1
direction: direction/forward
top level: BBa_I0466
start: 902
end: 942
orientation: inline

BBa_C0071
type: SequenceAnnotation
persistent identity: annotation943314
display id: annotation943314
version: 1
top level: BBa_I0466
location: https://synbiohub.org/public/igem/BBa_I0466/annotation943314/range943314/1
component: BBa_C0071

https://synbiohub.org/public/igem/BBa_I0466/annotation943314/range943314/1
type: Range
persistent identity: range943314
display id: range943314
version: 1
direction: direction/forward
top level: BBa_I0466
start: 19
end: 780
orientation: inline

BBa_K082032
type: ComponentDefinition
persistent identity: BBa K082032
display id: BBa_K082032
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: R0071-B0032-C0070
creator: Zhen Xu , Binjie Xu
created: 2008-10-27T12:00:00Z
modified: 2015-05-08T01:08:36Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _228_
m user id: 0
owner id: 1560
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: none
mutable notes: none
mutable provenance: none
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0032
component: BBa_C0070
component: BBa_R0071
sequence annotation: BBa_B0032
sequence annotation: BBa_R0071
sequence annotation: BBa_C0070
sequence: https://synbiohub.org/public/igem/BBa_K082032_sequence/1

BBa_B0032
type: Component
persistent identity: component2246226
display id: component2246226
version: 1
top level: BBa_K082032
access: public
definition: https://synbiohub.org/public/igem/BBa_B0032/1

BBa_C0070
type: Component
persistent identity: component2246231
display id: component2246231
version: 1
top level: BBa_K082032
access: public
definition: rhII

BBa_R0071
type: Component
persistent identity: component2246224
display id: component2246224
version: 1
top level: BBa_K082032
access: public
definition: https://synbiohub.org/public/igem/BBa_R0071/1

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation2246226
display id: annotation2246226
version: 1
top level: BBa_K082032
location: https://synbiohub.org/public/igem/BBa_K082032/annotation2246226/range2246226/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_K082032/annotation2246226/range2246226/1
type: Range
persistent identity: range2246226
display id: range2246226
version: 1
direction: direction/forward
top level: BBa_K082032
start: 62
end: 74
orientation: inline

BBa_R0071
type: SequenceAnnotation
persistent identity: annotation2246224
display id: annotation2246224
version: 1
top level: BBa_K082032
location: https://synbiohub.org/public/igem/BBa_K082032/annotation2246224/range2246224/1
component: BBa_R0071

https://synbiohub.org/public/igem/BBa_K082032/annotation2246224/range2246224/1
type: Range
persistent identity: range2246224
display id: range2246224
version: 1
direction: direction/reverse
top level: BBa_K082032
start: 1
end: 53
orientation: inline

BBa_C0070
type: SequenceAnnotation
persistent identity: annotation2246231
display id: annotation2246231
version: 1
top level: BBa_K082032
location: https://synbiohub.org/public/igem/BBa_K082032/annotation2246231/range2246231/1
component: BBa_C0070

https://synbiohub.org/public/igem/BBa_K082032/annotation2246231/range2246231/1
type: Range
persistent identity: range2246231
display id: range2246231
version: 1
direction: direction/reverse
top level: BBa_K082032
start: 81
end: 747
orientation: inline

BBa_I13208
type: ComponentDefinition
persistent identity: BBa I13208
display id: BBa_I13208
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: aiiA (LVA-) protein generator driven by plac
creator: Barry Canton
created: 2004-08-02T11:00:00Z
modified: 2015-08-31T04:07:33Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_6_
m user id: 0
owner id: 135
owning group id: 7
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Produces aiiA protein from the lac promoter. The aiiA is untagged and should be capable of degrading any intracellular AHL.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Signalling
role: SO:0000804
component: BBa_B0015
component: BBa_B0034
component: BBa_C0160
component: BBa_R0011
sequence annotation: BBa_B0034
sequence annotation: BBa_C0160
sequence annotation: BBa_R0011
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_I13208_sequence/1

BBa_B0015
type: Component
persistent identity: component2221601
display id: component2221601
version: 1
top level: BBa_I13208
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_B0034
type: Component
persistent identity: component2221592
display id: component2221592
version: 1
top level: BBa_I13208
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_C0160
type: Component
persistent identity: component2221594
display id: component2221594
version: 1
top level: BBa_I13208
access: public
definition: aiiA

BBa_R0011
type: Component
persistent identity: component2221586
display id: component2221586
version: 1
top level: BBa_I13208
access: public
definition: https://synbiohub.org/public/igem/BBa_R0011/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2221592
display id: annotation2221592
version: 1
top level: BBa_I13208
location: https://synbiohub.org/public/igem/BBa_I13208/annotation2221592/range2221592/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I13208/annotation2221592/range2221592/1
type: Range
persistent identity: range2221592
display id: range2221592
version: 1
direction: direction/reverse
top level: BBa_I13208
start: 64
end: 75
orientation: inline

BBa_C0160
type: SequenceAnnotation
persistent identity: annotation2221594
display id: annotation2221594
version: 1
top level: BBa_I13208
location: https://synbiohub.org/public/igem/BBa_I13208/annotation2221594/range2221594/1
component: BBa_C0160

https://synbiohub.org/public/igem/BBa_I13208/annotation2221594/range2221594/1
type: Range
persistent identity: range2221594
display id: range2221594
version: 1
direction: direction/reverse
top level: BBa_I13208
start: 82
end: 837
orientation: inline

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation2221586
display id: annotation2221586
version: 1
top level: BBa_I13208
location: https://synbiohub.org/public/igem/BBa_I13208/annotation2221586/range2221586/1
component: BBa_R0011

https://synbiohub.org/public/igem/BBa_I13208/annotation2221586/range2221586/1
type: Range
persistent identity: range2221586
display id: range2221586
version: 1
direction: direction/forward
top level: BBa_I13208
start: 1
end: 54
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2221601
display id: annotation2221601
version: 1
top level: BBa_I13208
location: https://synbiohub.org/public/igem/BBa_I13208/annotation2221601/range2221601/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_I13208/annotation2221601/range2221601/1
type: Range
persistent identity: range2221601
display id: range2221601
version: 1
direction: direction/reverse
top level: BBa_I13208
start: 846
end: 974
orientation: inline

BBa_K1520022
type: ComponentDefinition
persistent identity: BBa K1520022
display id: BBa_K1520022
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Pcons2-rbs-lacI-Ter-Plac-rbs-luxI-Ter-Pcons2-rbs-luxR-Ter-Prlux-rbs-rfp-rbs-luxI-Ter
creator: yue wu
created: 2014-09-21T11:00:00Z
modified: 2015-05-08T01:10:49Z
discontinued: false
dominant: false
group u list: _1902_
m user id: 0
owner id: 21318
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Plasmid in positive feedback verification: Pcons2-rbs-lacI-Ter-Plac-rbs-luxI-Ter-Pcons2-rbs-luxR-Ter-Prlux-rbs-rfp-rbs-luxI-Ter It is a positive feedback plasmid using luxI as key to feedback. Introducing IPTG will produce rfp and luxI, and luxI will work back to enhance production of rfp.
mutable notes: None
mutable provenance: Pcons2, lacI, Plac, luxI, luxR, Prlux, rfp, Ter
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1520020
component: BBa_K1520011
sequence annotation: BBa_K1520020
sequence annotation: BBa_K1520011
sequence: https://synbiohub.org/public/igem/BBa_K1520022_sequence/1

BBa_K1520020
type: Component
persistent identity: component2385176
display id: component2385176
version: 1
top level: BBa_K1520022
access: public
definition: BBa_K1520020

BBa_K1520011
type: Component
persistent identity: component2385187
display id: component2385187
version: 1
top level: BBa_K1520022
access: public
definition: BBa_K1520011

BBa_K1520020
type: SequenceAnnotation
persistent identity: annotation2385176
display id: annotation2385176
version: 1
top level: BBa_K1520022
location: https://synbiohub.org/public/igem/BBa_K1520022/annotation2385176/range2385176/1
component: BBa_K1520020

https://synbiohub.org/public/igem/BBa_K1520022/annotation2385176/range2385176/1
type: Range
persistent identity: range2385176
display id: range2385176
version: 1
direction: direction/reverse
top level: BBa_K1520022
start: 1
end: 3352
orientation: inline

BBa_K1520011
type: SequenceAnnotation
persistent identity: annotation2385187
display id: annotation2385187
version: 1
top level: BBa_K1520022
location: https://synbiohub.org/public/igem/BBa_K1520022/annotation2385187/range2385187/1
component: BBa_K1520011

https://synbiohub.org/public/igem/BBa_K1520022/annotation2385187/range2385187/1
type: Range
persistent identity: range2385187
display id: range2385187
version: 1
direction: direction/reverse
top level: BBa_K1520022
start: 3361
end: 4961
orientation: inline

BBa_K1520011
type: ComponentDefinition
persistent identity: BBa K1520011
display id: BBa_K1520011
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Prlux-rbs-rfp-rbs-luxI-Ter
creator: yue wu
created: 2014-09-21T11:00:00Z
modified: 2015-05-08T01:10:49Z
discontinued: false
dominant: false
group u list: _1902_
m user id: 0
owner id: 21318
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Prlux-rbs-rfp-rbs-luxI-Ter. It produces rfp and luxI when there is enough luxR an AHL.
mutable notes: None
mutable provenance: Prlux, rfp, luxI, Ter
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_R0062
component: BBa_K1520500
component: BBa_K1520504
component: BBa_K1520029
sequence annotation: BBa_K1520500
sequence annotation: BBa_K1520029
sequence annotation: BBa_R0062
sequence annotation: BBa_K1520504
sequence: https://synbiohub.org/public/igem/BBa_K1520011_sequence/1

BBa_R0062
type: Component
persistent identity: component2384957
display id: component2384957
version: 1
top level: BBa_K1520011
access: public
definition: https://synbiohub.org/public/igem/BBa_R0062/1

BBa_K1520500
type: Component
persistent identity: component2384962
display id: component2384962
version: 1
top level: BBa_K1520011
access: public
definition: https://synbiohub.org/public/igem/BBa_K1520500/1

BBa_K1520504
type: Component
persistent identity: component2384965
display id: component2384965
version: 1
top level: BBa_K1520011
access: public
definition: BBa_K1520504

BBa_K1520029
type: Component
persistent identity: component2384966
display id: component2384966
version: 1
top level: BBa_K1520011
access: public
definition: https://synbiohub.org/public/igem/BBa_K1520029/1

BBa_K1520500
type: SequenceAnnotation
persistent identity: annotation2384962
display id: annotation2384962
version: 1
top level: BBa_K1520011
location: https://synbiohub.org/public/igem/BBa_K1520011/annotation2384962/range2384962/1
component: BBa_K1520500

https://synbiohub.org/public/igem/BBa_K1520011/annotation2384962/range2384962/1
type: Range
persistent identity: range2384962
display id: range2384962
version: 1
direction: direction/reverse
top level: BBa_K1520011
start: 64
end: 795
orientation: inline

BBa_K1520029
type: SequenceAnnotation
persistent identity: annotation2384966
display id: annotation2384966
version: 1
top level: BBa_K1520011
location: https://synbiohub.org/public/igem/BBa_K1520011/annotation2384966/range2384966/1
component: BBa_K1520029

https://synbiohub.org/public/igem/BBa_K1520011/annotation2384966/range2384966/1
type: Range
persistent identity: range2384966
display id: range2384966
version: 1
direction: direction/reverse
top level: BBa_K1520011
start: 1473
end: 1601
orientation: inline

BBa_R0062
type: SequenceAnnotation
persistent identity: annotation2384957
display id: annotation2384957
version: 1
top level: BBa_K1520011
location: https://synbiohub.org/public/igem/BBa_K1520011/annotation2384957/range2384957/1
component: BBa_R0062

https://synbiohub.org/public/igem/BBa_K1520011/annotation2384957/range2384957/1
type: Range
persistent identity: range2384957
display id: range2384957
version: 1
direction: direction/forward
top level: BBa_K1520011
start: 1
end: 55
orientation: inline

BBa_K1520504
type: SequenceAnnotation
persistent identity: annotation2384965
display id: annotation2384965
version: 1
top level: BBa_K1520011
location: https://synbiohub.org/public/igem/BBa_K1520011/annotation2384965/range2384965/1
component: BBa_K1520504

https://synbiohub.org/public/igem/BBa_K1520011/annotation2384965/range2384965/1
type: Range
persistent identity: range2384965
display id: range2384965
version: 1
direction: direction/reverse
top level: BBa_K1520011
start: 804
end: 1464
orientation: inline

BBa_K091182
type: ComponentDefinition
persistent identity: BBa K091182
display id: BBa_K091182
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pLasR/cI-RBS-LuxI-RBS-Mnt-TT-pMnt/tetR-RBS-LuxI-RBS-cI-TT
creator: Robert Cool
created: 2008-08-04T11:00:00Z
modified: 2015-05-08T01:08:38Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _191_
m user id: 0
owner id: 3080
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This part is a combination of the two parts K091155 and K091144.
mutable notes: no spceial considerations
mutable provenance: These two parts contain parts that are in the registry and DNA built from oligos.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_K091155
component: BBa_K091144
sequence annotation: BBa_K091155
sequence annotation: BBa_K091144
sequence: https://synbiohub.org/public/igem/BBa_K091182_sequence/1

BBa_K091155
type: Component
persistent identity: component2301908
display id: component2301908
version: 1
top level: BBa_K091182
access: public
definition: https://synbiohub.org/public/igem/BBa_K091155/1

BBa_K091144
type: Component
persistent identity: component2301935
display id: component2301935
version: 1
top level: BBa_K091182
access: public
definition: https://synbiohub.org/public/igem/BBa_K091144/1

BBa_K091155
type: SequenceAnnotation
persistent identity: annotation2301908
display id: annotation2301908
version: 1
top level: BBa_K091182
location: https://synbiohub.org/public/igem/BBa_K091182/annotation2301908/range2301908/1
component: BBa_K091155

https://synbiohub.org/public/igem/BBa_K091182/annotation2301908/range2301908/1
type: Range
persistent identity: range2301908
display id: range2301908
version: 1
direction: direction/reverse
top level: BBa_K091182
start: 1
end: 1309
orientation: inline

BBa_K091144
type: SequenceAnnotation
persistent identity: annotation2301935
display id: annotation2301935
version: 1
top level: BBa_K091182
location: https://synbiohub.org/public/igem/BBa_K091182/annotation2301935/range2301935/1
component: BBa_K091144

https://synbiohub.org/public/igem/BBa_K091182/annotation2301935/range2301935/1
type: Range
persistent identity: range2301935
display id: range2301935
version: 1
direction: direction/reverse
top level: BBa_K091182
start: 1318
end: 2988
orientation: inline

BBa_K082031
type: ComponentDefinition
persistent identity: BBa K082031
display id: BBa_K082031
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: R0071-B0030-C0070
creator: Zhen Xu , Binjie Xu
created: 2008-10-27T12:00:00Z
modified: 2015-05-08T01:08:36Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _228_
m user id: 0
owner id: 1560
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: none
mutable notes: none
mutable provenance: none
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_C0070
component: BBa_R0071
component: BBa_B0030
sequence annotation: BBa_B0030
sequence annotation: BBa_R0071
sequence annotation: BBa_C0070
sequence: https://synbiohub.org/public/igem/BBa_K082031_sequence/1

BBa_C0070
type: Component
persistent identity: component2246243
display id: component2246243
version: 1
top level: BBa_K082031
access: public
definition: rhII

BBa_R0071
type: Component
persistent identity: component2246236
display id: component2246236
version: 1
top level: BBa_K082031
access: public
definition: https://synbiohub.org/public/igem/BBa_R0071/1

BBa_B0030
type: Component
persistent identity: component2246238
display id: component2246238
version: 1
top level: BBa_K082031
access: public
definition: https://synbiohub.org/public/igem/BBa_B0030/1

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation2246238
display id: annotation2246238
version: 1
top level: BBa_K082031
location: https://synbiohub.org/public/igem/BBa_K082031/annotation2246238/range2246238/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_K082031/annotation2246238/range2246238/1
type: Range
persistent identity: range2246238
display id: range2246238
version: 1
direction: direction/forward
top level: BBa_K082031
start: 62
end: 76
orientation: inline

BBa_R0071
type: SequenceAnnotation
persistent identity: annotation2246236
display id: annotation2246236
version: 1
top level: BBa_K082031
location: https://synbiohub.org/public/igem/BBa_K082031/annotation2246236/range2246236/1
component: BBa_R0071

https://synbiohub.org/public/igem/BBa_K082031/annotation2246236/range2246236/1
type: Range
persistent identity: range2246236
display id: range2246236
version: 1
direction: direction/reverse
top level: BBa_K082031
start: 1
end: 53
orientation: inline

BBa_C0070
type: SequenceAnnotation
persistent identity: annotation2246243
display id: annotation2246243
version: 1
top level: BBa_K082031
location: https://synbiohub.org/public/igem/BBa_K082031/annotation2246243/range2246243/1
component: BBa_C0070

https://synbiohub.org/public/igem/BBa_K082031/annotation2246243/range2246243/1
type: Range
persistent identity: range2246243
display id: range2246243
version: 1
direction: direction/reverse
top level: BBa_K082031
start: 83
end: 749
orientation: inline

BBa_K1315002
type: ComponentDefinition
persistent identity: BBa K1315002
display id: BBa_K1315002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: The rpfC gene from Xanthomonas campestris
creator: Dimitrios Michailidis
created: 2014-09-24T11:00:00Z
modified: 2015-05-08T01:09:48Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1690_
m user id: 0
owner id: 21812
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This is the coding sequence for the RpfC(regulation of pathogenicity factor C) protein. It is the sensor in the two-component signal transduction of Xcc that activates the regulator RpfG(Bba_K1315003) in the presence of DSF.
mutable notes: The sequence had 1 EcoRI site and 3 PstI sites that were all mutated out by site-directed mutagenesis.
mutable provenance: The source of this part is ''Xanthomonas campestris pv. campestris''(Xcc) genomic DNA which was provided by the Robert Ryan lab in the Molecular Microbiology department of the University of Dundee, UK.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: RpfC
sequence: https://synbiohub.org/public/igem/BBa_K1315002_sequence/1

RpfC
type: SequenceAnnotation
persistent identity: annotation2386328
display id: annotation2386328
version: 1
top level: BBa_K1315002
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1315002/annotation2386328/range2386328/1

https://synbiohub.org/public/igem/BBa_K1315002/annotation2386328/range2386328/1
type: Range
persistent identity: range2386328
display id: range2386328
version: 1
direction: direction/forward
top level: BBa_K1315002
start: 1
end: 2181
orientation: inline

BBa_K082037
type: ComponentDefinition
persistent identity: BBa K082037
display id: BBa_K082037
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RhlI & GFP generator
creator: Zhen Xu , Binjie Xu
created: 2008-10-27T12:00:00Z
modified: 2015-05-08T01:08:36Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _228_
m user id: 0
owner id: 1560
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: none
mutable notes: none
mutable provenance: none
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_E0840
component: BBa_R0040
component: BBa_B0034
component: BBa_C0070
sequence annotation: BBa_E0840
sequence annotation: BBa_R0040
sequence annotation: BBa_C0070
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K082037_sequence/1

BBa_E0840
type: Component
persistent identity: component2267209
display id: component2267209
version: 1
top level: BBa_K082037
access: public
definition: https://synbiohub.org/public/igem/BBa_E0840/1

BBa_R0040
type: Component
persistent identity: component2267188
display id: component2267188
version: 1
top level: BBa_K082037
access: public
definition: https://synbiohub.org/public/igem/BBa_R0040/1

BBa_B0034
type: Component
persistent identity: component2267194
display id: component2267194
version: 1
top level: BBa_K082037
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_C0070
type: Component
persistent identity: component2267198
display id: component2267198
version: 1
top level: BBa_K082037
access: public
definition: rhII

BBa_E0840
type: SequenceAnnotation
persistent identity: annotation2267209
display id: annotation2267209
version: 1
top level: BBa_K082037
location: https://synbiohub.org/public/igem/BBa_K082037/annotation2267209/range2267209/1
component: BBa_E0840

https://synbiohub.org/public/igem/BBa_K082037/annotation2267209/range2267209/1
type: Range
persistent identity: range2267209
display id: range2267209
version: 1
direction: direction/reverse
top level: BBa_K082037
start: 756
end: 1633
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2267188
display id: annotation2267188
version: 1
top level: BBa_K082037
location: https://synbiohub.org/public/igem/BBa_K082037/annotation2267188/range2267188/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_K082037/annotation2267188/range2267188/1
type: Range
persistent identity: range2267188
display id: range2267188
version: 1
direction: direction/forward
top level: BBa_K082037
start: 1
end: 54
orientation: inline

BBa_C0070
type: SequenceAnnotation
persistent identity: annotation2267198
display id: annotation2267198
version: 1
top level: BBa_K082037
location: https://synbiohub.org/public/igem/BBa_K082037/annotation2267198/range2267198/1
component: BBa_C0070

https://synbiohub.org/public/igem/BBa_K082037/annotation2267198/range2267198/1
type: Range
persistent identity: range2267198
display id: range2267198
version: 1
direction: direction/reverse
top level: BBa_K082037
start: 81
end: 747
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2267194
display id: annotation2267194
version: 1
top level: BBa_K082037
location: https://synbiohub.org/public/igem/BBa_K082037/annotation2267194/range2267194/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K082037/annotation2267194/range2267194/1
type: Range
persistent identity: range2267194
display id: range2267194
version: 1
direction: direction/reverse
top level: BBa_K082037
start: 63
end: 74
orientation: inline

BBa_K081009
type: ComponentDefinition
persistent identity: BBa K081009
display id: BBa_K081009
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: lasI protein generator (TERM-)
creator: Lorenzo Pasotti, Paolo Magni
created: 2008-10-17T11:00:00Z
modified: 2015-05-08T01:08:35Z
discontinued: false
dominant: false
experience: experience/None
group u list: _227_
m user id: 0
owner id: 2583
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: lasI: autoinducer synthetase for PAI from Pseudomonas aeruginosa. Strong RBS (efficiency=0.6).
mutable notes: We used BioBrick Standard Assembly.
mutable provenance: lasI: RBS:
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_C0078
component: BBa_B0030
sequence annotation: BBa_C0078
sequence annotation: BBa_B0030
sequence: https://synbiohub.org/public/igem/BBa_K081009_sequence/1

BBa_C0078
type: Component
persistent identity: component2244000
display id: component2244000
version: 1
top level: BBa_K081009
access: public
definition: lasI

BBa_B0030
type: Component
persistent identity: component2243995
display id: component2243995
version: 1
top level: BBa_K081009
access: public
definition: https://synbiohub.org/public/igem/BBa_B0030/1

BBa_C0078
type: SequenceAnnotation
persistent identity: annotation2244000
display id: annotation2244000
version: 1
top level: BBa_K081009
location: https://synbiohub.org/public/igem/BBa_K081009/annotation2244000/range2244000/1
component: BBa_C0078

https://synbiohub.org/public/igem/BBa_K081009/annotation2244000/range2244000/1
type: Range
persistent identity: range2244000
display id: range2244000
version: 1
direction: direction/reverse
top level: BBa_K081009
start: 22
end: 688
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation2243995
display id: annotation2243995
version: 1
top level: BBa_K081009
location: https://synbiohub.org/public/igem/BBa_K081009/annotation2243995/range2243995/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_K081009/annotation2243995/range2243995/1
type: Range
persistent identity: range2243995
display id: range2243995
version: 1
direction: direction/forward
top level: BBa_K081009
start: 1
end: 15
orientation: inline

BBa_F2620
type: ComponentDefinition
persistent identity: BBa F2620
display id: BBa_F2620
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: 3OC6HSL -> PoPS Receiver
creator: Barry Canton [bcanton@mit.edu] and Anna Labno [labnoa@mit.edu]
created: 2004-08-08T11:00:00Z
modified: 2015-08-31T04:07:27Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _101_6_
m user id: 0
owner id: 135
owning group id: 6
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This device senses the 3OC6HSL concentration in the media and produces output PoPS according to the transfer function given below. LuxR production is controlled by the tet repressible R0040. Initial characterization work has been done on this device using I13273 as a test device.
mutable notes: Example Data Sheets for F2620
mutable provenance: Formerly I13270
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Signalling
role: SO:0000804
component: BBa_R0040
component: BBa_B0034
component: BBa_B0015
component: BBa_C0062
component: BBa_R0062
sequence annotation: BBa_R0040
sequence annotation: BBa_B0015
sequence annotation: BBa_B0034
sequence annotation: BBa_R0062
sequence annotation: BBa_C0062
sequence: https://synbiohub.org/public/igem/BBa_F2620_sequence/1

BBa_R0040
type: Component
persistent identity: component2259889
display id: component2259889
version: 1
top level: BBa_F2620
access: public
definition: https://synbiohub.org/public/igem/BBa_R0040/1

BBa_B0034
type: Component
persistent identity: component2259895
display id: component2259895
version: 1
top level: BBa_F2620
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0015
type: Component
persistent identity: component2259908
display id: component2259908
version: 1
top level: BBa_F2620
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_C0062
type: Component
persistent identity: component2259898
display id: component2259898
version: 1
top level: BBa_F2620
access: public
definition: luxr

BBa_R0062
type: Component
persistent identity: component2259910
display id: component2259910
version: 1
top level: BBa_F2620
access: public
definition: https://synbiohub.org/public/igem/BBa_R0062/1

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2259889
display id: annotation2259889
version: 1
top level: BBa_F2620
location: https://synbiohub.org/public/igem/BBa_F2620/annotation2259889/range2259889/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_F2620/annotation2259889/range2259889/1
type: Range
persistent identity: range2259889
display id: range2259889
version: 1
direction: direction/forward
top level: BBa_F2620
start: 1
end: 54
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2259908
display id: annotation2259908
version: 1
top level: BBa_F2620
location: https://synbiohub.org/public/igem/BBa_F2620/annotation2259908/range2259908/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_F2620/annotation2259908/range2259908/1
type: Range
persistent identity: range2259908
display id: range2259908
version: 1
direction: direction/reverse
top level: BBa_F2620
start: 870
end: 998
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2259895
display id: annotation2259895
version: 1
top level: BBa_F2620
location: https://synbiohub.org/public/igem/BBa_F2620/annotation2259895/range2259895/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_F2620/annotation2259895/range2259895/1
type: Range
persistent identity: range2259895
display id: range2259895
version: 1
direction: direction/reverse
top level: BBa_F2620
start: 63
end: 74
orientation: inline

BBa_R0062
type: SequenceAnnotation
persistent identity: annotation2259910
display id: annotation2259910
version: 1
top level: BBa_F2620
location: https://synbiohub.org/public/igem/BBa_F2620/annotation2259910/range2259910/1
component: BBa_R0062

https://synbiohub.org/public/igem/BBa_F2620/annotation2259910/range2259910/1
type: Range
persistent identity: range2259910
display id: range2259910
version: 1
direction: direction/forward
top level: BBa_F2620
start: 1007
end: 1061
orientation: inline

BBa_C0062
type: SequenceAnnotation
persistent identity: annotation2259898
display id: annotation2259898
version: 1
top level: BBa_F2620
location: https://synbiohub.org/public/igem/BBa_F2620/annotation2259898/range2259898/1
component: BBa_C0062

https://synbiohub.org/public/igem/BBa_F2620/annotation2259898/range2259898/1
type: Range
persistent identity: range2259898
display id: range2259898
version: 1
direction: direction/forward
top level: BBa_F2620
start: 81
end: 836
orientation: inline

BBa_K1520009
type: ComponentDefinition
persistent identity: BBa K1520009
display id: BBa_K1520009
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Plac-rbs-luxI-Ter
creator: yue wu
created: 2014-09-21T11:00:00Z
modified: 2015-05-08T01:10:49Z
discontinued: false
dominant: false
group u list: _1902_
m user id: 0
owner id: 21318
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Plac-rbs-luxI-Ter. It produces luxI when there is enough IPTG or less lacI.
mutable notes: None
mutable provenance: Plac, luxI, Ter
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_R0010
component: BBa_K1520029
component: BBa_K1520504
sequence annotation: BBa_K1520029
sequence annotation: BBa_R0010
sequence annotation: BBa_K1520504
sequence: https://synbiohub.org/public/igem/BBa_K1520009_sequence/1

BBa_R0010
type: Component
persistent identity: component2384937
display id: component2384937
version: 1
top level: BBa_K1520009
access: public
definition: https://synbiohub.org/public/igem/BBa_R0010/1

BBa_K1520029
type: Component
persistent identity: component2384947
display id: component2384947
version: 1
top level: BBa_K1520009
access: public
definition: https://synbiohub.org/public/igem/BBa_K1520029/1

BBa_K1520504
type: Component
persistent identity: component2384946
display id: component2384946
version: 1
top level: BBa_K1520009
access: public
definition: BBa_K1520504

BBa_K1520029
type: SequenceAnnotation
persistent identity: annotation2384947
display id: annotation2384947
version: 1
top level: BBa_K1520009
location: https://synbiohub.org/public/igem/BBa_K1520009/annotation2384947/range2384947/1
component: BBa_K1520029

https://synbiohub.org/public/igem/BBa_K1520009/annotation2384947/range2384947/1
type: Range
persistent identity: range2384947
display id: range2384947
version: 1
direction: direction/reverse
top level: BBa_K1520009
start: 878
end: 1006
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation2384937
display id: annotation2384937
version: 1
top level: BBa_K1520009
location: https://synbiohub.org/public/igem/BBa_K1520009/annotation2384937/range2384937/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_K1520009/annotation2384937/range2384937/1
type: Range
persistent identity: range2384937
display id: range2384937
version: 1
direction: direction/forward
top level: BBa_K1520009
start: 1
end: 200
orientation: inline

BBa_K1520504
type: SequenceAnnotation
persistent identity: annotation2384946
display id: annotation2384946
version: 1
top level: BBa_K1520009
location: https://synbiohub.org/public/igem/BBa_K1520009/annotation2384946/range2384946/1
component: BBa_K1520504

https://synbiohub.org/public/igem/BBa_K1520009/annotation2384946/range2384946/1
type: Range
persistent identity: range2384946
display id: range2384946
version: 1
direction: direction/reverse
top level: BBa_K1520009
start: 209
end: 869
orientation: inline

BBa_K783054
type: ComponentDefinition
persistent identity: BBa K783054
display id: BBa_K783054
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: This is a MoClo converted version of BBa_C0079
creator: Traci Haddock
created: 2012-10-01T11:00:00Z
modified: 2015-05-08T01:13:21Z
discontinued: false
dominant: false
group u list: _1038_
m user id: 0
owner id: 8248
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This is a MoClo converted version of BBa_C0079, which is the coding region for the lasR protein, which accepts chemical signal AI-1 (made by lasI protein). It has two MoClo fusion sites flanking it.
mutable notes: We had to design the proper MoClo fusion sites to flank this part.
mutable provenance: BBa_C0079
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: G
sequence annotation: LVA
sequence annotation: lasR
sequence: https://synbiohub.org/public/igem/BBa_K783054_sequence/1

G
type: SequenceAnnotation
persistent identity: annotation2214300
display id: annotation2214300
version: 1
top level: BBa_K783054
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K783054/annotation2214300/range2214300/1

https://synbiohub.org/public/igem/BBa_K783054/annotation2214300/range2214300/1
type: Range
persistent identity: range2214300
display id: range2214300
version: 1
direction: direction/forward
top level: BBa_K783054
start: 480
end: 480
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation2214301
display id: annotation2214301
version: 1
top level: BBa_K783054
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K783054/annotation2214301/range2214301/1

https://synbiohub.org/public/igem/BBa_K783054/annotation2214301/range2214301/1
type: Range
persistent identity: range2214301
display id: range2214301
version: 1
direction: direction/forward
top level: BBa_K783054
start: 718
end: 750
orientation: inline

lasR
type: SequenceAnnotation
persistent identity: annotation2214299
display id: annotation2214299
version: 1
top level: BBa_K783054
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K783054/annotation2214299/range2214299/1

https://synbiohub.org/public/igem/BBa_K783054/annotation2214299/range2214299/1
type: Range
persistent identity: range2214299
display id: range2214299
version: 1
direction: direction/forward
top level: BBa_K783054
start: 1
end: 717
orientation: inline

BBa_I13272
type: ComponentDefinition
persistent identity: BBa I13272
display id: BBa_I13272
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: YFP Producer Controlled by 3OC6HSL Receiver Device
creator: Barry Canton
created: 2004-11-23T12:00:00Z
modified: 2015-08-31T04:07:33Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _101_6_
m user id: 0
owner id: 135
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This device is composed of a pLuxL promoter drivinga 3OC6HSL receiver device. The output PoPS is used to produce YFP The YFP should be lightly tagged. This is the exact receiver in the MIT SBC04 synchronization device. It is identical to the previously characterized I13263 but with a tagged output device
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Signalling
role: SO:0000804
component: BBa_E0034
component: BBa_B0010
component: BBa_R0062
component: BBa_B0012
component: BBa_R0063
component: BBa_C0062
component: BBa_B0034
sequence annotation: BBa_B0010
sequence annotation: BBa_E0034
sequence annotation: BBa_B0034
sequence annotation: BBa_R0063
sequence annotation: BBa_R0062
sequence annotation: BBa_C0062
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_I13272_sequence/1

BBa_E0034
type: Component
persistent identity: component1484881
display id: component1484881
version: 1
top level: BBa_I13272
access: public
definition: https://synbiohub.org/public/igem/BBa_E0034/1

BBa_B0010
type: Component
persistent identity: component1484886
display id: component1484886
version: 1
top level: BBa_I13272
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0010
type: Component
persistent identity: component1484840
display id: component1484840
version: 1
top level: BBa_I13272
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_R0062
type: Component
persistent identity: component1484865
display id: component1484865
version: 1
top level: BBa_I13272
access: public
definition: https://synbiohub.org/public/igem/BBa_R0062/1

BBa_B0012
type: Component
persistent identity: component1484850
display id: component1484850
version: 1
top level: BBa_I13272
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_R0063
type: Component
persistent identity: component1484808
display id: component1484808
version: 1
top level: BBa_I13272
access: public
definition: https://synbiohub.org/public/igem/BBa_R0063/1

BBa_C0062
type: Component
persistent identity: component1484832
display id: component1484832
version: 1
top level: BBa_I13272
access: public
definition: luxr

BBa_B0034
type: Component
persistent identity: component1484873
display id: component1484873
version: 1
top level: BBa_I13272
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0034
type: Component
persistent identity: component1484817
display id: component1484817
version: 1
top level: BBa_I13272
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0012
type: Component
persistent identity: component1484896
display id: component1484896
version: 1
top level: BBa_I13272
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1484886
display id: annotation1484886
version: 1
top level: BBa_I13272
location: https://synbiohub.org/public/igem/BBa_I13272/annotation1484886/range1484886/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I13272/annotation1484886/range1484886/1
type: Range
persistent identity: range1484886
display id: range1484886
version: 1
direction: direction/forward
top level: BBa_I13272
start: 1955
end: 2034
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1484840
display id: annotation1484840
version: 1
top level: BBa_I13272
location: https://synbiohub.org/public/igem/BBa_I13272/annotation1484840/range1484840/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I13272/annotation1484840/range1484840/1
type: Range
persistent identity: range1484840
display id: range1484840
version: 1
direction: direction/forward
top level: BBa_I13272
start: 967
end: 1046
orientation: inline

BBa_E0034
type: SequenceAnnotation
persistent identity: annotation1484881
display id: annotation1484881
version: 1
top level: BBa_I13272
location: https://synbiohub.org/public/igem/BBa_I13272/annotation1484881/range1484881/1
component: BBa_E0034

https://synbiohub.org/public/igem/BBa_I13272/annotation1484881/range1484881/1
type: Range
persistent identity: range1484881
display id: range1484881
version: 1
direction: direction/forward
top level: BBa_I13272
start: 1185
end: 1946
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1484873
display id: annotation1484873
version: 1
top level: BBa_I13272
location: https://synbiohub.org/public/igem/BBa_I13272/annotation1484873/range1484873/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I13272/annotation1484873/range1484873/1
type: Range
persistent identity: range1484873
display id: range1484873
version: 1
direction: direction/forward
top level: BBa_I13272
start: 1167
end: 1178
orientation: inline

BBa_R0063
type: SequenceAnnotation
persistent identity: annotation1484808
display id: annotation1484808
version: 1
top level: BBa_I13272
location: https://synbiohub.org/public/igem/BBa_I13272/annotation1484808/range1484808/1
component: BBa_R0063

https://synbiohub.org/public/igem/BBa_I13272/annotation1484808/range1484808/1
type: Range
persistent identity: range1484808
display id: range1484808
version: 1
direction: direction/forward
top level: BBa_I13272
start: 1
end: 151
orientation: inline

BBa_R0062
type: SequenceAnnotation
persistent identity: annotation1484865
display id: annotation1484865
version: 1
top level: BBa_I13272
location: https://synbiohub.org/public/igem/BBa_I13272/annotation1484865/range1484865/1
component: BBa_R0062

https://synbiohub.org/public/igem/BBa_I13272/annotation1484865/range1484865/1
type: Range
persistent identity: range1484865
display id: range1484865
version: 1
direction: direction/forward
top level: BBa_I13272
start: 1104
end: 1158
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1484817
display id: annotation1484817
version: 1
top level: BBa_I13272
location: https://synbiohub.org/public/igem/BBa_I13272/annotation1484817/range1484817/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I13272/annotation1484817/range1484817/1
type: Range
persistent identity: range1484817
display id: range1484817
version: 1
direction: direction/forward
top level: BBa_I13272
start: 160
end: 171
orientation: inline

BBa_C0062
type: SequenceAnnotation
persistent identity: annotation1484832
display id: annotation1484832
version: 1
top level: BBa_I13272
location: https://synbiohub.org/public/igem/BBa_I13272/annotation1484832/range1484832/1
component: BBa_C0062

https://synbiohub.org/public/igem/BBa_I13272/annotation1484832/range1484832/1
type: Range
persistent identity: range1484832
display id: range1484832
version: 1
direction: direction/forward
top level: BBa_I13272
start: 178
end: 933
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1484850
display id: annotation1484850
version: 1
top level: BBa_I13272
location: https://synbiohub.org/public/igem/BBa_I13272/annotation1484850/range1484850/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I13272/annotation1484850/range1484850/1
type: Range
persistent identity: range1484850
display id: range1484850
version: 1
direction: direction/forward
top level: BBa_I13272
start: 1055
end: 1095
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1484896
display id: annotation1484896
version: 1
top level: BBa_I13272
location: https://synbiohub.org/public/igem/BBa_I13272/annotation1484896/range1484896/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I13272/annotation1484896/range1484896/1
type: Range
persistent identity: range1484896
display id: range1484896
version: 1
direction: direction/forward
top level: BBa_I13272
start: 2043
end: 2083
orientation: inline

BBa_J07012
type: ComponentDefinition
persistent identity: BBa J07012
display id: BBa_J07012
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: scFv anti-fluorescein [4-4-20]
creator: mit_igem_2005
created: 2005-07-07T11:00:00Z
modified: 2015-08-31T04:08:19Z
discontinued: false
dominant: false
experience: experience/None
group u list: _21_
m user id: 0
owner id: 363
owning group id: 21
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: single chain Antibody fragment selected to bind to fluorescein molecules. This scFv is the weakest of the three specified sequences. DNA insert in the pCT-4-4-20 (also known as pcT-302) from NheI to XhoI
mutable provenance: Substantial Energetic Improvement with Minimal Structural Perturbation in a High Affinity Mutant Antibody
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Signalling
role: SO:0000804
sequence annotation: CDR1 VL
sequence annotation: CDR2 VH
sequence annotation: CDR1 VH
sequence annotation: CDR3 VH
sequence annotation: VL
sequence annotation: CDR3 VL
sequence annotation: VH
sequence annotation: Linker
sequence annotation: CDR2 VL
sequence: https://synbiohub.org/public/igem/BBa_J07012_sequence/1

CDR1 VL
type: SequenceAnnotation
persistent identity: annotation1581982
display id: annotation1581982
version: 1
top level: BBa_J07012
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J07012/annotation1581982/range1581982/1

https://synbiohub.org/public/igem/BBa_J07012/annotation1581982/range1581982/1
type: Range
persistent identity: range1581982
display id: range1581982
version: 1
direction: direction/forward
top level: BBa_J07012
start: 61
end: 120
orientation: inline

CDR2 VH
type: SequenceAnnotation
persistent identity: annotation1582062
display id: annotation1582062
version: 1
top level: BBa_J07012
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J07012/annotation1582062/range1582062/1

https://synbiohub.org/public/igem/BBa_J07012/annotation1582062/range1582062/1
type: Range
persistent identity: range1582062
display id: range1582062
version: 1
direction: direction/forward
top level: BBa_J07012
start: 559
end: 615
orientation: inline

CDR1 VH
type: SequenceAnnotation
persistent identity: annotation1582016
display id: annotation1582016
version: 1
top level: BBa_J07012
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J07012/annotation1582016/range1582016/1

https://synbiohub.org/public/igem/BBa_J07012/annotation1582016/range1582016/1
type: Range
persistent identity: range1582016
display id: range1582016
version: 1
direction: direction/forward
top level: BBa_J07012
start: 502
end: 513
orientation: inline

CDR3 VH
type: SequenceAnnotation
persistent identity: annotation1582123
display id: annotation1582123
version: 1
top level: BBa_J07012
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J07012/annotation1582123/range1582123/1

https://synbiohub.org/public/igem/BBa_J07012/annotation1582123/range1582123/1
type: Range
persistent identity: range1582123
display id: range1582123
version: 1
direction: direction/forward
top level: BBa_J07012
start: 711
end: 732
orientation: inline

VL
type: SequenceAnnotation
persistent identity: annotation1582130
display id: annotation1582130
version: 1
top level: BBa_J07012
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J07012/annotation1582130/range1582130/1

https://synbiohub.org/public/igem/BBa_J07012/annotation1582130/range1582130/1
type: Range
persistent identity: range1582130
display id: range1582130
version: 1
direction: direction/forward
top level: BBa_J07012
start: 1
end: 336
orientation: inline

CDR3 VL
type: SequenceAnnotation
persistent identity: annotation1581987
display id: annotation1581987
version: 1
top level: BBa_J07012
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J07012/annotation1581987/range1581987/1

https://synbiohub.org/public/igem/BBa_J07012/annotation1581987/range1581987/1
type: Range
persistent identity: range1581987
display id: range1581987
version: 1
direction: direction/forward
top level: BBa_J07012
start: 280
end: 306
orientation: inline

VH
type: SequenceAnnotation
persistent identity: annotation1582131
display id: annotation1582131
version: 1
top level: BBa_J07012
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J07012/annotation1582131/range1582131/1

https://synbiohub.org/public/igem/BBa_J07012/annotation1582131/range1582131/1
type: Range
persistent identity: range1582131
display id: range1582131
version: 1
direction: direction/forward
top level: BBa_J07012
start: 412
end: 762
orientation: inline

Linker
type: SequenceAnnotation
persistent identity: annotation1582003
display id: annotation1582003
version: 1
top level: BBa_J07012
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J07012/annotation1582003/range1582003/1

https://synbiohub.org/public/igem/BBa_J07012/annotation1582003/range1582003/1
type: Range
persistent identity: range1582003
display id: range1582003
version: 1
direction: direction/forward
top level: BBa_J07012
start: 337
end: 411
orientation: inline

CDR2 VL
type: SequenceAnnotation
persistent identity: annotation1581986
display id: annotation1581986
version: 1
top level: BBa_J07012
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J07012/annotation1581986/range1581986/1

https://synbiohub.org/public/igem/BBa_J07012/annotation1581986/range1581986/1
type: Range
persistent identity: range1581986
display id: range1581986
version: 1
direction: direction/forward
top level: BBa_J07012
start: 163
end: 183
orientation: inline

BBa_I13263
type: ComponentDefinition
persistent identity: BBa I13263
display id: BBa_I13263
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Lux Receiver (HSL & R0063 driven)
creator: Barry Canton
created: 2004-08-08T11:00:00Z
modified: 2015-08-31T04:07:33Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _101_6_
m user id: 0
owner id: 135
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Composite part based on the A340620 PoPS amplifier. pLuxL promoter driving luxR-based HSL receiver. pLux-R is driving a YFP output device.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Signalling
role: SO:0000804
component: BBa_R0063
component: BBa_B0034
component: BBa_C0062
component: BBa_R0062
component: BBa_B0010
component: BBa_B0012
component: BBa_E0030
sequence annotation: BBa_B0034
sequence annotation: BBa_B0012
sequence annotation: BBa_R0063
sequence annotation: BBa_R0062
sequence annotation: BBa_C0062
sequence annotation: BBa_E0030
sequence annotation: BBa_B0010
sequence: https://synbiohub.org/public/igem/BBa_I13263_sequence/1

BBa_R0063
type: Component
persistent identity: component1059056
display id: component1059056
version: 1
top level: BBa_I13263
access: public
definition: https://synbiohub.org/public/igem/BBa_R0063/1

BBa_B0034
type: Component
persistent identity: component1059065
display id: component1059065
version: 1
top level: BBa_I13263
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0034
type: Component
persistent identity: component1059121
display id: component1059121
version: 1
top level: BBa_I13263
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_C0062
type: Component
persistent identity: component1059080
display id: component1059080
version: 1
top level: BBa_I13263
access: public
definition: luxr

BBa_R0062
type: Component
persistent identity: component1059113
display id: component1059113
version: 1
top level: BBa_I13263
access: public
definition: https://synbiohub.org/public/igem/BBa_R0062/1

BBa_B0010
type: Component
persistent identity: component1059128
display id: component1059128
version: 1
top level: BBa_I13263
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
type: Component
persistent identity: component1059138
display id: component1059138
version: 1
top level: BBa_I13263
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_E0030
type: Component
persistent identity: component1059123
display id: component1059123
version: 1
top level: BBa_I13263
access: public
definition: https://synbiohub.org/public/igem/BBa_E0030/1

BBa_B0012
type: Component
persistent identity: component1059098
display id: component1059098
version: 1
top level: BBa_I13263
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0010
type: Component
persistent identity: component1059088
display id: component1059088
version: 1
top level: BBa_I13263
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1059121
display id: annotation1059121
version: 1
top level: BBa_I13263
location: https://synbiohub.org/public/igem/BBa_I13263/annotation1059121/range1059121/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I13263/annotation1059121/range1059121/1
type: Range
persistent identity: range1059121
display id: range1059121
version: 1
direction: direction/forward
top level: BBa_I13263
start: 1167
end: 1178
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1059065
display id: annotation1059065
version: 1
top level: BBa_I13263
location: https://synbiohub.org/public/igem/BBa_I13263/annotation1059065/range1059065/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I13263/annotation1059065/range1059065/1
type: Range
persistent identity: range1059065
display id: range1059065
version: 1
direction: direction/forward
top level: BBa_I13263
start: 160
end: 171
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1059138
display id: annotation1059138
version: 1
top level: BBa_I13263
location: https://synbiohub.org/public/igem/BBa_I13263/annotation1059138/range1059138/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I13263/annotation1059138/range1059138/1
type: Range
persistent identity: range1059138
display id: range1059138
version: 1
direction: direction/forward
top level: BBa_I13263
start: 2004
end: 2044
orientation: inline

BBa_R0063
type: SequenceAnnotation
persistent identity: annotation1059056
display id: annotation1059056
version: 1
top level: BBa_I13263
location: https://synbiohub.org/public/igem/BBa_I13263/annotation1059056/range1059056/1
component: BBa_R0063

https://synbiohub.org/public/igem/BBa_I13263/annotation1059056/range1059056/1
type: Range
persistent identity: range1059056
display id: range1059056
version: 1
direction: direction/forward
top level: BBa_I13263
start: 1
end: 151
orientation: inline

BBa_R0062
type: SequenceAnnotation
persistent identity: annotation1059113
display id: annotation1059113
version: 1
top level: BBa_I13263
location: https://synbiohub.org/public/igem/BBa_I13263/annotation1059113/range1059113/1
component: BBa_R0062

https://synbiohub.org/public/igem/BBa_I13263/annotation1059113/range1059113/1
type: Range
persistent identity: range1059113
display id: range1059113
version: 1
direction: direction/forward
top level: BBa_I13263
start: 1104
end: 1158
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1059098
display id: annotation1059098
version: 1
top level: BBa_I13263
location: https://synbiohub.org/public/igem/BBa_I13263/annotation1059098/range1059098/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I13263/annotation1059098/range1059098/1
type: Range
persistent identity: range1059098
display id: range1059098
version: 1
direction: direction/forward
top level: BBa_I13263
start: 1055
end: 1095
orientation: inline

BBa_C0062
type: SequenceAnnotation
persistent identity: annotation1059080
display id: annotation1059080
version: 1
top level: BBa_I13263
location: https://synbiohub.org/public/igem/BBa_I13263/annotation1059080/range1059080/1
component: BBa_C0062

https://synbiohub.org/public/igem/BBa_I13263/annotation1059080/range1059080/1
type: Range
persistent identity: range1059080
display id: range1059080
version: 1
direction: direction/forward
top level: BBa_I13263
start: 178
end: 933
orientation: inline

BBa_E0030
type: SequenceAnnotation
persistent identity: annotation1059123
display id: annotation1059123
version: 1
top level: BBa_I13263
location: https://synbiohub.org/public/igem/BBa_I13263/annotation1059123/range1059123/1
component: BBa_E0030

https://synbiohub.org/public/igem/BBa_I13263/annotation1059123/range1059123/1
type: Range
persistent identity: range1059123
display id: range1059123
version: 1
direction: direction/forward
top level: BBa_I13263
start: 1185
end: 1907
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1059088
display id: annotation1059088
version: 1
top level: BBa_I13263
location: https://synbiohub.org/public/igem/BBa_I13263/annotation1059088/range1059088/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I13263/annotation1059088/range1059088/1
type: Range
persistent identity: range1059088
display id: range1059088
version: 1
direction: direction/forward
top level: BBa_I13263
start: 967
end: 1046
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1059128
display id: annotation1059128
version: 1
top level: BBa_I13263
location: https://synbiohub.org/public/igem/BBa_I13263/annotation1059128/range1059128/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I13263/annotation1059128/range1059128/1
type: Range
persistent identity: range1059128
display id: range1059128
version: 1
direction: direction/forward
top level: BBa_I13263
start: 1916
end: 1995
orientation: inline

aiiA
type: ComponentDefinition
persistent identity: BBa C0160
display id: BBa_C0160
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: autoinducer inactivation enzyme aiiA (no LVA)
creator: jcbraff
created: 2004-05-26T11:00:00Z
modified: 2015-08-31T04:07:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_1_
m user id: 0
owner id: 61
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: same as C0060 except no LVA tag
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: aiiA
sequence: https://synbiohub.org/public/igem/BBa_C0160_sequence/1

aiiA
type: SequenceAnnotation
persistent identity: annotation1891585
display id: annotation1891585
version: 1
top level: aiiA
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_C0160/annotation1891585/range1891585/1

https://synbiohub.org/public/igem/BBa_C0160/annotation1891585/range1891585/1
type: Range
persistent identity: range1891585
display id: range1891585
version: 1
direction: direction/forward
top level: aiiA
start: 1
end: 756
orientation: inline

BBa_I729006
type: ComponentDefinition
persistent identity: BBa I729006
display id: BBa_I729006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: AHL reporter and aiia device
creator: Hiroki Fukutomi
created: 2007-10-24T11:00:00Z
modified: 2015-08-31T04:07:55Z
discontinued: false
dominant: false
experience: experience/None
group u list: _117_
m user id: 0
owner id: 1585
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: LuxR and aiiA express constantly. AiiA inactivate AHL as signaling molecule. If AHL concentration exceed the capacity of aiiA, GFP expresses.
mutable provenance: Biobrick -> Ligation
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_S01656
component: BBa_T9002
sequence annotation: BBa_S01656
sequence annotation: BBa_T9002
sequence: https://synbiohub.org/public/igem/BBa_I729006_sequence/1

BBa_S01656
type: Component
persistent identity: component2305117
display id: component2305117
version: 1
top level: BBa_I729006
access: public
definition: https://synbiohub.org/public/igem/BBa_S01656/1

BBa_T9002
type: Component
persistent identity: component2305096
display id: component2305096
version: 1
top level: BBa_I729006
access: public
definition: BBa_T9002

BBa_S01656
type: SequenceAnnotation
persistent identity: annotation2305117
display id: annotation2305117
version: 1
top level: BBa_I729006
location: https://synbiohub.org/public/igem/BBa_I729006/annotation2305117/range2305117/1
component: BBa_S01656

https://synbiohub.org/public/igem/BBa_I729006/annotation2305117/range2305117/1
type: Range
persistent identity: range2305117
display id: range2305117
version: 1
direction: direction/reverse
top level: BBa_I729006
start: 1954
end: 2985
orientation: inline

BBa_T9002
type: SequenceAnnotation
persistent identity: annotation2305096
display id: annotation2305096
version: 1
top level: BBa_I729006
location: https://synbiohub.org/public/igem/BBa_I729006/annotation2305096/range2305096/1
component: BBa_T9002

https://synbiohub.org/public/igem/BBa_I729006/annotation2305096/range2305096/1
type: Range
persistent identity: range2305096
display id: range2305096
version: 1
direction: direction/reverse
top level: BBa_I729006
start: 1
end: 1945
orientation: inline

BBa_I722011
type: ComponentDefinition
persistent identity: BBa I722011
display id: BBa_I722011
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: rhl producer followed by a AHL acceptor
creator: Bo Hu
created: 2007-10-20T11:00:00Z
modified: 2015-08-31T04:07:53Z
discontinued: false
dominant: false
experience: experience/None
group u list: _150_
m user id: 0
owner id: 1325
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: this part could accept AHLs and also could produce mRFP easily to detect
mutable notes: ligate the mRFP producer and AHL acceptor together
mutable provenance: Based on the parts provided by MIT
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_I0405
component: BBa_F2620
sequence annotation: BBa_F2620
sequence annotation: BBa_I0405
sequence: https://synbiohub.org/public/igem/BBa_I722011_sequence/1

BBa_I0405
type: Component
persistent identity: component2299180
display id: component2299180
version: 1
top level: BBa_I722011
access: public
definition: https://synbiohub.org/public/igem/BBa_I0405/1

BBa_F2620
type: Component
persistent identity: component2299206
display id: component2299206
version: 1
top level: BBa_I722011
access: public
definition: BBa_F2620

BBa_F2620
type: SequenceAnnotation
persistent identity: annotation2299206
display id: annotation2299206
version: 1
top level: BBa_I722011
location: https://synbiohub.org/public/igem/BBa_I722011/annotation2299206/range2299206/1
component: BBa_F2620

https://synbiohub.org/public/igem/BBa_I722011/annotation2299206/range2299206/1
type: Range
persistent identity: range2299206
display id: range2299206
version: 1
direction: direction/reverse
top level: BBa_I722011
start: 1642
end: 2702
orientation: inline

BBa_I0405
type: SequenceAnnotation
persistent identity: annotation2299180
display id: annotation2299180
version: 1
top level: BBa_I722011
location: https://synbiohub.org/public/igem/BBa_I722011/annotation2299180/range2299180/1
component: BBa_I0405

https://synbiohub.org/public/igem/BBa_I722011/annotation2299180/range2299180/1
type: Range
persistent identity: range2299180
display id: range2299180
version: 1
direction: direction/reverse
top level: BBa_I722011
start: 1
end: 1633
orientation: inline

BBa_K1520020
type: ComponentDefinition
persistent identity: BBa K1520020
display id: BBa_K1520020
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Pcons2-rbs-lacI-Ter-Plac-rbs-luxI-Ter-Pcons2-rbs-luxR-Ter
creator: yue wu
created: 2014-09-21T11:00:00Z
modified: 2015-05-08T01:10:49Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1902_
m user id: 0
owner id: 21318
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Pcons2-rbs-lacI-Ter-Plac-rbs-luxI-Ter It produces luxI when there is enough IPTG.
mutable notes: None
mutable provenance: Pcons2, lacI, Plac, luxI, Ter
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1520003
component: BBa_K1520019
sequence annotation: BBa_K1520019
sequence annotation: BBa_K1520003
sequence: https://synbiohub.org/public/igem/BBa_K1520020_sequence/1

BBa_K1520003
type: Component
persistent identity: component2385116
display id: component2385116
version: 1
top level: BBa_K1520020
access: public
definition: BBa_K1520003

BBa_K1520019
type: Component
persistent identity: component2385110
display id: component2385110
version: 1
top level: BBa_K1520020
access: public
definition: BBa_K1520019

BBa_K1520019
type: SequenceAnnotation
persistent identity: annotation2385110
display id: annotation2385110
version: 1
top level: BBa_K1520020
location: https://synbiohub.org/public/igem/BBa_K1520020/annotation2385110/range2385110/1
component: BBa_K1520019

https://synbiohub.org/public/igem/BBa_K1520020/annotation2385110/range2385110/1
type: Range
persistent identity: range2385110
display id: range2385110
version: 1
direction: direction/reverse
top level: BBa_K1520020
start: 1
end: 2365
orientation: inline

BBa_K1520003
type: SequenceAnnotation
persistent identity: annotation2385116
display id: annotation2385116
version: 1
top level: BBa_K1520020
location: https://synbiohub.org/public/igem/BBa_K1520020/annotation2385116/range2385116/1
component: BBa_K1520003

https://synbiohub.org/public/igem/BBa_K1520020/annotation2385116/range2385116/1
type: Range
persistent identity: range2385116
display id: range2385116
version: 1
direction: direction/reverse
top level: BBa_K1520020
start: 2374
end: 3352
orientation: inline

BBa_K091134
type: ComponentDefinition
persistent identity: BBa K091134
display id: BBa_K091134
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Las Receiver with GFP reporter
creator: James Barron
created: 2008-07-12T11:00:00Z
modified: 2015-05-08T01:08:38Z
discontinued: false
dominant: false
experience: experience/None
group u list: _191_
m user id: 0
owner id: 3026
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This device receives AI-1, produces LasR, and reports its production with GFP
mutable notes: none
mutable provenance: comes from existing parts in the Registry (S03966 + S03956)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_S03956
component: BBa_S03966
sequence annotation: BBa_S03966
sequence annotation: BBa_S03956
sequence: https://synbiohub.org/public/igem/BBa_K091134_sequence/1

BBa_S03956
type: Component
persistent identity: component2281266
display id: component2281266
version: 1
top level: BBa_K091134
access: public
definition: https://synbiohub.org/public/igem/BBa_S03956/1

BBa_S03966
type: Component
persistent identity: component2281249
display id: component2281249
version: 1
top level: BBa_K091134
access: public
definition: https://synbiohub.org/public/igem/BBa_S03966/1

BBa_S03966
type: SequenceAnnotation
persistent identity: annotation2281249
display id: annotation2281249
version: 1
top level: BBa_K091134
location: https://synbiohub.org/public/igem/BBa_K091134/annotation2281249/range2281249/1
component: BBa_S03966

https://synbiohub.org/public/igem/BBa_K091134/annotation2281249/range2281249/1
type: Range
persistent identity: range2281249
display id: range2281249
version: 1
direction: direction/reverse
top level: BBa_K091134
start: 1
end: 999
orientation: inline

BBa_S03956
type: SequenceAnnotation
persistent identity: annotation2281266
display id: annotation2281266
version: 1
top level: BBa_K091134
location: https://synbiohub.org/public/igem/BBa_K091134/annotation2281266/range2281266/1
component: BBa_S03956

https://synbiohub.org/public/igem/BBa_K091134/annotation2281266/range2281266/1
type: Range
persistent identity: range2281266
display id: range2281266
version: 1
direction: direction/reverse
top level: BBa_K091134
start: 1008
end: 2048
orientation: inline

BBa_I13018
type: ComponentDefinition
persistent identity: BBa I13018
display id: BBa_I13018
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LuxR Cassette under Ptet (Other)
creator: mit
created: 2004-07-12T11:00:00Z
modified: 2015-08-31T04:07:32Z
discontinued: false
dominant: false
experience: experience/None
group u list: _6_
m user id: 0
owner id: 101
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Ptet R0040. LuxR Cassette I0462
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Signalling
role: SO:0000804
component: BBa_B0012
component: BBa_C0062
component: BBa_B0010
component: BBa_B0034
component: BBa_R0040
sequence annotation: BBa_B0012
sequence annotation: BBa_B0010
sequence annotation: BBa_C0062
sequence annotation: BBa_B0034
sequence annotation: BBa_R0040
sequence: https://synbiohub.org/public/igem/BBa_I13018_sequence/1

BBa_B0012
type: Component
persistent identity: component947078
display id: component947078
version: 1
top level: BBa_I13018
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_C0062
type: Component
persistent identity: component947060
display id: component947060
version: 1
top level: BBa_I13018
access: public
definition: luxr

BBa_B0010
type: Component
persistent identity: component947068
display id: component947068
version: 1
top level: BBa_I13018
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0034
type: Component
persistent identity: component947045
display id: component947045
version: 1
top level: BBa_I13018
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_R0040
type: Component
persistent identity: component947037
display id: component947037
version: 1
top level: BBa_I13018
access: public
definition: https://synbiohub.org/public/igem/BBa_R0040/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation947078
display id: annotation947078
version: 1
top level: BBa_I13018
location: https://synbiohub.org/public/igem/BBa_I13018/annotation947078/range947078/1
component: BBa_B0012

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type: Range
persistent identity: range947078
display id: range947078
version: 1
direction: direction/forward
top level: BBa_I13018
start: 958
end: 998
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation947068
display id: annotation947068
version: 1
top level: BBa_I13018
location: https://synbiohub.org/public/igem/BBa_I13018/annotation947068/range947068/1
component: BBa_B0010

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type: Range
persistent identity: range947068
display id: range947068
version: 1
direction: direction/forward
top level: BBa_I13018
start: 870
end: 949
orientation: inline

BBa_C0062
type: SequenceAnnotation
persistent identity: annotation947060
display id: annotation947060
version: 1
top level: BBa_I13018
location: https://synbiohub.org/public/igem/BBa_I13018/annotation947060/range947060/1
component: BBa_C0062

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type: Range
persistent identity: range947060
display id: range947060
version: 1
direction: direction/forward
top level: BBa_I13018
start: 81
end: 836
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation947045
display id: annotation947045
version: 1
top level: BBa_I13018
location: https://synbiohub.org/public/igem/BBa_I13018/annotation947045/range947045/1
component: BBa_B0034

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type: Range
persistent identity: range947045
display id: range947045
version: 1
direction: direction/forward
top level: BBa_I13018
start: 63
end: 74
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation947037
display id: annotation947037
version: 1
top level: BBa_I13018
location: https://synbiohub.org/public/igem/BBa_I13018/annotation947037/range947037/1
component: BBa_R0040

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type: Range
persistent identity: range947037
display id: range947037
version: 1
direction: direction/forward
top level: BBa_I13018
start: 1
end: 54
orientation: inline

BBa_K091203
type: ComponentDefinition
persistent identity: BBa K091203
display id: BBa_K091203
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pBAD-RBS-LuxR-RBS-LacI(I12)-TT
creator: Todd Eckdahl
created: 2008-09-03T11:00:00Z
modified: 2015-05-08T01:08:39Z
discontinued: false
dominant: false
experience: experience/None
group u list: _191_
m user id: 0
owner id: 606
owning group id: 61
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This expression casette constitutively produces the LuxR regulatory protein that can respond to the quorum sensing molecule 3OC6. It also produces a LacI repressor with a mutation at position 12 that results in an isoleucine. The mutant repressor has been shown to have an increased affinity for its operator.
mutable notes: None.
mutable provenance: Intermediate parts.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_S04058
component: BBa_K091166
sequence annotation: BBa_S04058
sequence annotation: BBa_K091166
sequence: https://synbiohub.org/public/igem/BBa_K091203_sequence/1

BBa_S04058
type: Component
persistent identity: component2266907
display id: component2266907
version: 1
top level: BBa_K091203
access: public
definition: https://synbiohub.org/public/igem/BBa_S04058/1

BBa_K091166
type: Component
persistent identity: component2266896
display id: component2266896
version: 1
top level: BBa_K091203
access: public
definition: https://synbiohub.org/public/igem/BBa_K091166/1

BBa_S04058
type: SequenceAnnotation
persistent identity: annotation2266907
display id: annotation2266907
version: 1
top level: BBa_K091203
location: https://synbiohub.org/public/igem/BBa_K091203/annotation2266907/range2266907/1
component: BBa_S04058

https://synbiohub.org/public/igem/BBa_K091203/annotation2266907/range2266907/1
type: Range
persistent identity: range2266907
display id: range2266907
version: 1
direction: direction/reverse
top level: BBa_K091203
start: 946
end: 2183
orientation: inline

BBa_K091166
type: SequenceAnnotation
persistent identity: annotation2266896
display id: annotation2266896
version: 1
top level: BBa_K091203
location: https://synbiohub.org/public/igem/BBa_K091203/annotation2266896/range2266896/1
component: BBa_K091166

https://synbiohub.org/public/igem/BBa_K091203/annotation2266896/range2266896/1
type: Range
persistent identity: range2266896
display id: range2266896
version: 1
direction: direction/reverse
top level: BBa_K091203
start: 1
end: 937
orientation: inline

BBa_I13264
type: ComponentDefinition
persistent identity: BBa I13264
display id: BBa_I13264
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Lux based Sender/Receiver/Reporter system
creator: Barry Canton
created: 2004-08-08T11:00:00Z
modified: 2015-08-31T04:07:33Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_6_
m user id: 0
owner id: 135
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Analogue of the V. Fischeri quorum-sensing system. Built using Biobrick components. Includes LuxR and LuxI under the control of their natural promoters and includes a YFP output device on the same transcript as LuxI
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Signalling
role: SO:0000804
component: BBa_B0010
component: BBa_E0030
component: BBa_R0063
component: BBa_R0062
component: BBa_B0012
component: BBa_C0061
component: BBa_C0062
component: BBa_B0034
sequence annotation: BBa_R0062
sequence annotation: BBa_C0061
sequence annotation: BBa_B0034
sequence annotation: BBa_B0012
sequence annotation: BBa_E0030
sequence annotation: BBa_B0010
sequence annotation: BBa_C0062
sequence annotation: BBa_R0063
sequence: https://synbiohub.org/public/igem/BBa_I13264_sequence/1

BBa_B0010
type: Component
persistent identity: component1039774
display id: component1039774
version: 1
top level: BBa_I13264
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_E0030
type: Component
persistent identity: component1039826
display id: component1039826
version: 1
top level: BBa_I13264
access: public
definition: https://synbiohub.org/public/igem/BBa_E0030/1

BBa_R0063
type: Component
persistent identity: component1039742
display id: component1039742
version: 1
top level: BBa_I13264
access: public
definition: https://synbiohub.org/public/igem/BBa_R0063/1

BBa_R0062
type: Component
persistent identity: component1039799
display id: component1039799
version: 1
top level: BBa_I13264
access: public
definition: https://synbiohub.org/public/igem/BBa_R0062/1

BBa_B0012
type: Component
persistent identity: component1039784
display id: component1039784
version: 1
top level: BBa_I13264
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_C0061
type: Component
persistent identity: component1039817
display id: component1039817
version: 1
top level: BBa_I13264
access: public
definition: luxI

BBa_C0062
type: Component
persistent identity: component1039766
display id: component1039766
version: 1
top level: BBa_I13264
access: public
definition: luxr

BBa_B0010
type: Component
persistent identity: component1039831
display id: component1039831
version: 1
top level: BBa_I13264
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0034
type: Component
persistent identity: component1039751
display id: component1039751
version: 1
top level: BBa_I13264
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0012
type: Component
persistent identity: component1039841
display id: component1039841
version: 1
top level: BBa_I13264
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0034
type: Component
persistent identity: component1039824
display id: component1039824
version: 1
top level: BBa_I13264
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0034
type: Component
persistent identity: component1039807
display id: component1039807
version: 1
top level: BBa_I13264
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_R0062
type: SequenceAnnotation
persistent identity: annotation1039799
display id: annotation1039799
version: 1
top level: BBa_I13264
location: https://synbiohub.org/public/igem/BBa_I13264/annotation1039799/range1039799/1
component: BBa_R0062

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type: Range
persistent identity: range1039799
display id: range1039799
version: 1
direction: direction/forward
top level: BBa_I13264
start: 1104
end: 1158
orientation: inline

BBa_C0061
type: SequenceAnnotation
persistent identity: annotation1039817
display id: annotation1039817
version: 1
top level: BBa_I13264
location: https://synbiohub.org/public/igem/BBa_I13264/annotation1039817/range1039817/1
component: BBa_C0061

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type: Range
persistent identity: range1039817
display id: range1039817
version: 1
direction: direction/forward
top level: BBa_I13264
start: 1185
end: 1802
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1039807
display id: annotation1039807
version: 1
top level: BBa_I13264
location: https://synbiohub.org/public/igem/BBa_I13264/annotation1039807/range1039807/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I13264/annotation1039807/range1039807/1
type: Range
persistent identity: range1039807
display id: range1039807
version: 1
direction: direction/forward
top level: BBa_I13264
start: 1167
end: 1178
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1039824
display id: annotation1039824
version: 1
top level: BBa_I13264
location: https://synbiohub.org/public/igem/BBa_I13264/annotation1039824/range1039824/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I13264/annotation1039824/range1039824/1
type: Range
persistent identity: range1039824
display id: range1039824
version: 1
direction: direction/forward
top level: BBa_I13264
start: 1836
end: 1847
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1039784
display id: annotation1039784
version: 1
top level: BBa_I13264
location: https://synbiohub.org/public/igem/BBa_I13264/annotation1039784/range1039784/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I13264/annotation1039784/range1039784/1
type: Range
persistent identity: range1039784
display id: range1039784
version: 1
direction: direction/forward
top level: BBa_I13264
start: 1055
end: 1095
orientation: inline

BBa_E0030
type: SequenceAnnotation
persistent identity: annotation1039826
display id: annotation1039826
version: 1
top level: BBa_I13264
location: https://synbiohub.org/public/igem/BBa_I13264/annotation1039826/range1039826/1
component: BBa_E0030

https://synbiohub.org/public/igem/BBa_I13264/annotation1039826/range1039826/1
type: Range
persistent identity: range1039826
display id: range1039826
version: 1
direction: direction/forward
top level: BBa_I13264
start: 1854
end: 2576
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1039831
display id: annotation1039831
version: 1
top level: BBa_I13264
location: https://synbiohub.org/public/igem/BBa_I13264/annotation1039831/range1039831/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I13264/annotation1039831/range1039831/1
type: Range
persistent identity: range1039831
display id: range1039831
version: 1
direction: direction/forward
top level: BBa_I13264
start: 2585
end: 2664
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1039751
display id: annotation1039751
version: 1
top level: BBa_I13264
location: https://synbiohub.org/public/igem/BBa_I13264/annotation1039751/range1039751/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I13264/annotation1039751/range1039751/1
type: Range
persistent identity: range1039751
display id: range1039751
version: 1
direction: direction/forward
top level: BBa_I13264
start: 160
end: 171
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1039774
display id: annotation1039774
version: 1
top level: BBa_I13264
location: https://synbiohub.org/public/igem/BBa_I13264/annotation1039774/range1039774/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I13264/annotation1039774/range1039774/1
type: Range
persistent identity: range1039774
display id: range1039774
version: 1
direction: direction/forward
top level: BBa_I13264
start: 967
end: 1046
orientation: inline

BBa_C0062
type: SequenceAnnotation
persistent identity: annotation1039766
display id: annotation1039766
version: 1
top level: BBa_I13264
location: https://synbiohub.org/public/igem/BBa_I13264/annotation1039766/range1039766/1
component: BBa_C0062

https://synbiohub.org/public/igem/BBa_I13264/annotation1039766/range1039766/1
type: Range
persistent identity: range1039766
display id: range1039766
version: 1
direction: direction/forward
top level: BBa_I13264
start: 178
end: 933
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1039841
display id: annotation1039841
version: 1
top level: BBa_I13264
location: https://synbiohub.org/public/igem/BBa_I13264/annotation1039841/range1039841/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I13264/annotation1039841/range1039841/1
type: Range
persistent identity: range1039841
display id: range1039841
version: 1
direction: direction/forward
top level: BBa_I13264
start: 2673
end: 2713
orientation: inline

BBa_R0063
type: SequenceAnnotation
persistent identity: annotation1039742
display id: annotation1039742
version: 1
top level: BBa_I13264
location: https://synbiohub.org/public/igem/BBa_I13264/annotation1039742/range1039742/1
component: BBa_R0063

https://synbiohub.org/public/igem/BBa_I13264/annotation1039742/range1039742/1
type: Range
persistent identity: range1039742
display id: range1039742
version: 1
direction: direction/forward
top level: BBa_I13264
start: 1
end: 151
orientation: inline

pCI-mCherr
type: ComponentDefinition
persistent identity: BBa K1157013
display id: BBa_K1157013
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pCI-mCherry-pConst-RhlR-pRhl-RhlR-CI
creator: Lin Ang Chieh
created: 2013-09-14T11:00:00Z
modified: 2015-05-08T01:09:30Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1469_
m user id: 0
owner id: 16435
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This biosensor is constructed to sense C-4 AHL molecules through a method different from BBa_K1157012. This time when AHL molecules are sensed, it produces Rhl receptors which initiates production of CI. Therefore, CI inhibits pCI and the expression of reporter mCherry. When compared to control group, the results will be expected to express lower fluorescence level when there is existence of AHL molecules.
mutable notes: The parts are built by the original biobricks provided by iGEM official, and there are no restriction enzyme sites in the inner part of the construct.
mutable provenance: This is built by the biobricks provided by iGEM official
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_J23100
component: BBa_C0171
component: BBa_B0015
component: BBa_B0030
component: BBa_C0051
component: BBa_J06504
component: BBa_R0051
component: BBa_R0071
sequence annotation: BBa_C0051
sequence annotation: BBa_B0015
sequence annotation: BBa_B0030
sequence annotation: BBa_R0071
sequence annotation: BBa_J23100
sequence annotation: BBa_C0171
sequence annotation: BBa_R0051
sequence annotation: BBa_J06504
sequence: https://synbiohub.org/public/igem/BBa_K1157013_sequence/1

BBa_J23100
type: Component
persistent identity: component2357796
display id: component2357796
version: 1
top level: pCI-mCherr
access: public
definition: https://synbiohub.org/public/igem/BBa_J23100/1

BBa_C0171
type: Component
persistent identity: component2357815
display id: component2357815
version: 1
top level: pCI-mCherr
access: public
definition: rhIR

BBa_B0015
type: Component
persistent identity: component2357829
display id: component2357829
version: 1
top level: pCI-mCherr
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_C0171
type: Component
persistent identity: component2357800
display id: component2357800
version: 1
top level: pCI-mCherr
access: public
definition: rhIR

BBa_B0030
type: Component
persistent identity: component2357798
display id: component2357798
version: 1
top level: pCI-mCherr
access: public
definition: https://synbiohub.org/public/igem/BBa_B0030/1

BBa_B0015
type: Component
persistent identity: component2357795
display id: component2357795
version: 1
top level: pCI-mCherr
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_C0051
type: Component
persistent identity: component2357822
display id: component2357822
version: 1
top level: pCI-mCherr
access: public
definition: https://synbiohub.org/public/igem/BBa_C0051/1

BBa_J06504
type: Component
persistent identity: component2357788
display id: component2357788
version: 1
top level: pCI-mCherr
access: public
definition: https://synbiohub.org/public/igem/BBa_J06504/1

BBa_B0030
type: Component
persistent identity: component2357817
display id: component2357817
version: 1
top level: pCI-mCherr
access: public
definition: https://synbiohub.org/public/igem/BBa_B0030/1

BBa_R0051
type: Component
persistent identity: component2357779
display id: component2357779
version: 1
top level: pCI-mCherr
access: public
definition: https://synbiohub.org/public/igem/BBa_R0051/1

BBa_B0030
type: Component
persistent identity: component2357784
display id: component2357784
version: 1
top level: pCI-mCherr
access: public
definition: https://synbiohub.org/public/igem/BBa_B0030/1

BBa_R0071
type: Component
persistent identity: component2357811
display id: component2357811
version: 1
top level: pCI-mCherr
access: public
definition: https://synbiohub.org/public/igem/BBa_R0071/1

BBa_B0015
type: Component
persistent identity: component2357807
display id: component2357807
version: 1
top level: pCI-mCherr
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_B0030
type: Component
persistent identity: component2357813
display id: component2357813
version: 1
top level: pCI-mCherr
access: public
definition: https://synbiohub.org/public/igem/BBa_B0030/1

BBa_C0051
type: SequenceAnnotation
persistent identity: annotation2357822
display id: annotation2357822
version: 1
top level: pCI-mCherr
location: https://synbiohub.org/public/igem/BBa_K1157013/annotation2357822/range2357822/1
component: BBa_C0051

https://synbiohub.org/public/igem/BBa_K1157013/annotation2357822/range2357822/1
type: Range
persistent identity: range2357822
display id: range2357822
version: 1
direction: direction/reverse
top level: pCI-mCherr
start: 2716
end: 3490
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2357795
display id: annotation2357795
version: 1
top level: pCI-mCherr
location: https://synbiohub.org/public/igem/BBa_K1157013/annotation2357795/range2357795/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K1157013/annotation2357795/range2357795/1
type: Range
persistent identity: range2357795
display id: range2357795
version: 1
direction: direction/reverse
top level: pCI-mCherr
start: 801
end: 929
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation2357798
display id: annotation2357798
version: 1
top level: pCI-mCherr
location: https://synbiohub.org/public/igem/BBa_K1157013/annotation2357798/range2357798/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_K1157013/annotation2357798/range2357798/1
type: Range
persistent identity: range2357798
display id: range2357798
version: 1
direction: direction/forward
top level: pCI-mCherr
start: 981
end: 995
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation2357784
display id: annotation2357784
version: 1
top level: pCI-mCherr
location: https://synbiohub.org/public/igem/BBa_K1157013/annotation2357784/range2357784/1
component: BBa_B0030

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type: Range
persistent identity: range2357784
display id: range2357784
version: 1
direction: direction/forward
top level: pCI-mCherr
start: 58
end: 72
orientation: inline

BBa_R0071
type: SequenceAnnotation
persistent identity: annotation2357811
display id: annotation2357811
version: 1
top level: pCI-mCherr
location: https://synbiohub.org/public/igem/BBa_K1157013/annotation2357811/range2357811/1
component: BBa_R0071

https://synbiohub.org/public/igem/BBa_K1157013/annotation2357811/range2357811/1
type: Range
persistent identity: range2357811
display id: range2357811
version: 1
direction: direction/reverse
top level: pCI-mCherr
start: 1876
end: 1928
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2357829
display id: annotation2357829
version: 1
top level: pCI-mCherr
location: https://synbiohub.org/public/igem/BBa_K1157013/annotation2357829/range2357829/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K1157013/annotation2357829/range2357829/1
type: Range
persistent identity: range2357829
display id: range2357829
version: 1
direction: direction/reverse
top level: pCI-mCherr
start: 3499
end: 3627
orientation: inline

BBa_J23100
type: SequenceAnnotation
persistent identity: annotation2357796
display id: annotation2357796
version: 1
top level: pCI-mCherr
location: https://synbiohub.org/public/igem/BBa_K1157013/annotation2357796/range2357796/1
component: BBa_J23100

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type: Range
persistent identity: range2357796
display id: range2357796
version: 1
direction: direction/reverse
top level: pCI-mCherr
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orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2357807
display id: annotation2357807
version: 1
top level: pCI-mCherr
location: https://synbiohub.org/public/igem/BBa_K1157013/annotation2357807/range2357807/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K1157013/annotation2357807/range2357807/1
type: Range
persistent identity: range2357807
display id: range2357807
version: 1
direction: direction/reverse
top level: pCI-mCherr
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orientation: inline

BBa_C0171
type: SequenceAnnotation
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display id: annotation2357800
version: 1
top level: pCI-mCherr
location: https://synbiohub.org/public/igem/BBa_K1157013/annotation2357800/range2357800/1
component: BBa_C0171

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type: Range
persistent identity: range2357800
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direction: direction/reverse
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orientation: inline

BBa_B0030
type: SequenceAnnotation
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display id: annotation2357817
version: 1
top level: pCI-mCherr
location: https://synbiohub.org/public/igem/BBa_K1157013/annotation2357817/range2357817/1
component: BBa_B0030

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type: Range
persistent identity: range2357817
display id: range2357817
version: 1
direction: direction/forward
top level: pCI-mCherr
start: 2695
end: 2709
orientation: inline

BBa_B0030
type: SequenceAnnotation
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display id: annotation2357813
version: 1
top level: pCI-mCherr
location: https://synbiohub.org/public/igem/BBa_K1157013/annotation2357813/range2357813/1
component: BBa_B0030

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display id: range2357813
version: 1
direction: direction/forward
top level: pCI-mCherr
start: 1937
end: 1951
orientation: inline

BBa_R0051
type: SequenceAnnotation
persistent identity: annotation2357779
display id: annotation2357779
version: 1
top level: pCI-mCherr
location: https://synbiohub.org/public/igem/BBa_K1157013/annotation2357779/range2357779/1
component: BBa_R0051

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type: Range
persistent identity: range2357779
display id: range2357779
version: 1
direction: direction/forward
top level: pCI-mCherr
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orientation: inline

BBa_J06504
type: SequenceAnnotation
persistent identity: annotation2357788
display id: annotation2357788
version: 1
top level: pCI-mCherr
location: https://synbiohub.org/public/igem/BBa_K1157013/annotation2357788/range2357788/1
component: BBa_J06504

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type: Range
persistent identity: range2357788
display id: range2357788
version: 1
direction: direction/reverse
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start: 79
end: 792
orientation: inline

BBa_C0171
type: SequenceAnnotation
persistent identity: annotation2357815
display id: annotation2357815
version: 1
top level: pCI-mCherr
location: https://synbiohub.org/public/igem/BBa_K1157013/annotation2357815/range2357815/1
component: BBa_C0171

https://synbiohub.org/public/igem/BBa_K1157013/annotation2357815/range2357815/1
type: Range
persistent identity: range2357815
display id: range2357815
version: 1
direction: direction/reverse
top level: pCI-mCherr
start: 1958
end: 2686
orientation: inline

BBa_K574005
type: ComponentDefinition
persistent identity: BBa K574005
display id: BBa_K574005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: 3OC12HSL and YFP regulated by pBad
creator: Lei Wei
created: 2011-09-28T11:00:00Z
modified: 2015-05-08T01:12:44Z
discontinued: false
dominant: false
experience: experience/None
group u list: _745_
m user id: 0
owner id: 8529
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: pBAD + lasI + yfp.
mutable notes: Empty.
mutable provenance: Exsiting Parts.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Signalling
role: SO:0000804
component: BBa_K081009
component: BBa_I13453
component: BBa_E0430
sequence annotation: BBa_K081009
sequence annotation: BBa_I13453
sequence annotation: BBa_E0430
sequence: https://synbiohub.org/public/igem/BBa_K574005_sequence/1

BBa_K081009
type: Component
persistent identity: component2268879
display id: component2268879
version: 1
top level: BBa_K574005
access: public
definition: BBa_K081009

BBa_I13453
type: Component
persistent identity: component2268871
display id: component2268871
version: 1
top level: BBa_K574005
access: public
definition: https://synbiohub.org/public/igem/BBa_I13453/1

BBa_E0430
type: Component
persistent identity: component2268889
display id: component2268889
version: 1
top level: BBa_K574005
access: public
definition: https://synbiohub.org/public/igem/BBa_E0430/1

BBa_K081009
type: SequenceAnnotation
persistent identity: annotation2268879
display id: annotation2268879
version: 1
top level: BBa_K574005
location: https://synbiohub.org/public/igem/BBa_K574005/annotation2268879/range2268879/1
component: BBa_K081009

https://synbiohub.org/public/igem/BBa_K574005/annotation2268879/range2268879/1
type: Range
persistent identity: range2268879
display id: range2268879
version: 1
direction: direction/reverse
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start: 139
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orientation: inline

BBa_I13453
type: SequenceAnnotation
persistent identity: annotation2268871
display id: annotation2268871
version: 1
top level: BBa_K574005
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persistent identity: range2268871
display id: range2268871
version: 1
direction: direction/reverse
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orientation: inline

BBa_E0430
type: SequenceAnnotation
persistent identity: annotation2268889
display id: annotation2268889
version: 1
top level: BBa_K574005
location: https://synbiohub.org/public/igem/BBa_K574005/annotation2268889/range2268889/1
component: BBa_E0430

https://synbiohub.org/public/igem/BBa_K574005/annotation2268889/range2268889/1
type: Range
persistent identity: range2268889
display id: range2268889
version: 1
direction: direction/reverse
top level: BBa_K574005
start: 835
end: 1712
orientation: inline

BBa_I732923
type: ComponentDefinition
persistent identity: BBa I732923
display id: BBa_I732923
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: I732907 I732922
creator: Zhan Jian
created: 2007-10-19T11:00:00Z
modified: 2015-08-31T04:08:00Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_I732922
component: BBa_I732907
sequence annotation: BBa_I732922
sequence annotation: BBa_I732907
sequence: https://synbiohub.org/public/igem/BBa_I732923_sequence/1

BBa_I732922
type: Component
persistent identity: component2321440
display id: component2321440
version: 1
top level: BBa_I732923
access: public
definition: https://synbiohub.org/public/igem/BBa_I732922/1

BBa_I732907
type: Component
persistent identity: component2321387
display id: component2321387
version: 1
top level: BBa_I732923
access: public
definition: https://synbiohub.org/public/igem/BBa_I732907/1

BBa_I732922
type: SequenceAnnotation
persistent identity: annotation2321440
display id: annotation2321440
version: 1
top level: BBa_I732923
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component: BBa_I732922

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type: Range
persistent identity: range2321440
display id: range2321440
version: 1
direction: direction/reverse
top level: BBa_I732923
start: 1382
end: 5682
orientation: inline

BBa_I732907
type: SequenceAnnotation
persistent identity: annotation2321387
display id: annotation2321387
version: 1
top level: BBa_I732923
location: https://synbiohub.org/public/igem/BBa_I732923/annotation2321387/range2321387/1
component: BBa_I732907

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type: Range
persistent identity: range2321387
display id: range2321387
version: 1
direction: direction/reverse
top level: BBa_I732923
start: 1
end: 1373
orientation: inline

Rec-Open
type: ComponentDefinition
persistent identity: BBa I726081
display id: BBa_I726081
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Rec-LRY.RC
creator: Bangalore iGEM
created: 2007-07-03T11:00:00Z
modified: 2015-08-31T04:07:55Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _107_
m user id: 0
owner id: 2077
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: Abcdefghijkl...
mutable notes: none
mutable provenance: Registry
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_E0030
component: BBa_B0012
component: BBa_B0034
component: BBa_B0010
component: BBa_R0062
component: BBa_R0011
component: BBa_C0062
component: BBa_E0020
sequence annotation: BBa_R0011
sequence annotation: BBa_E0030
sequence annotation: BBa_B0012
sequence annotation: BBa_B0034
sequence annotation: BBa_E0020
sequence annotation: BBa_B0010
sequence annotation: BBa_R0062
sequence annotation: BBa_C0062
sequence: https://synbiohub.org/public/igem/BBa_I726081_sequence/1

BBa_E0030
type: Component
persistent identity: component2296176
display id: component2296176
version: 1
top level: Rec-Open
access: public
definition: https://synbiohub.org/public/igem/BBa_E0030/1

BBa_B0012
type: Component
persistent identity: component2296193
display id: component2296193
version: 1
top level: Rec-Open
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0034
type: Component
persistent identity: component2296167
display id: component2296167
version: 1
top level: Rec-Open
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0010
type: Component
persistent identity: component2296191
display id: component2296191
version: 1
top level: Rec-Open
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
type: Component
persistent identity: component2296179
display id: component2296179
version: 1
top level: Rec-Open
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0034
type: Component
persistent identity: component2296189
display id: component2296189
version: 1
top level: Rec-Open
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0010
type: Component
persistent identity: component2296177
display id: component2296177
version: 1
top level: Rec-Open
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_R0062
type: Component
persistent identity: component2296184
display id: component2296184
version: 1
top level: Rec-Open
access: public
definition: https://synbiohub.org/public/igem/BBa_R0062/1

BBa_R0011
type: Component
persistent identity: component2296161
display id: component2296161
version: 1
top level: Rec-Open
access: public
definition: https://synbiohub.org/public/igem/BBa_R0011/1

BBa_B0034
type: Component
persistent identity: component2296175
display id: component2296175
version: 1
top level: Rec-Open
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_C0062
type: Component
persistent identity: component2296170
display id: component2296170
version: 1
top level: Rec-Open
access: public
definition: luxr

BBa_E0020
type: Component
persistent identity: component2296190
display id: component2296190
version: 1
top level: Rec-Open
access: public
definition: https://synbiohub.org/public/igem/BBa_E0020/1

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation2296161
display id: annotation2296161
version: 1
top level: Rec-Open
location: https://synbiohub.org/public/igem/BBa_I726081/annotation2296161/range2296161/1
component: BBa_R0011

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type: Range
persistent identity: range2296161
display id: range2296161
version: 1
direction: direction/forward
top level: Rec-Open
start: 1
end: 54
orientation: inline

BBa_E0030
type: SequenceAnnotation
persistent identity: annotation2296176
display id: annotation2296176
version: 1
top level: Rec-Open
location: https://synbiohub.org/public/igem/BBa_I726081/annotation2296176/range2296176/1
component: BBa_E0030

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type: Range
persistent identity: range2296176
display id: range2296176
version: 1
direction: direction/reverse
top level: Rec-Open
start: 889
end: 1611
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2296179
display id: annotation2296179
version: 1
top level: Rec-Open
location: https://synbiohub.org/public/igem/BBa_I726081/annotation2296179/range2296179/1
component: BBa_B0012

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type: Range
persistent identity: range2296179
display id: range2296179
version: 1
direction: direction/forward
top level: Rec-Open
start: 1708
end: 1748
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2296189
display id: annotation2296189
version: 1
top level: Rec-Open
location: https://synbiohub.org/public/igem/BBa_I726081/annotation2296189/range2296189/1
component: BBa_B0034

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type: Range
persistent identity: range2296189
display id: range2296189
version: 1
direction: direction/reverse
top level: Rec-Open
start: 1820
end: 1831
orientation: inline

BBa_E0020
type: SequenceAnnotation
persistent identity: annotation2296190
display id: annotation2296190
version: 1
top level: Rec-Open
location: https://synbiohub.org/public/igem/BBa_I726081/annotation2296190/range2296190/1
component: BBa_E0020

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type: Range
persistent identity: range2296190
display id: range2296190
version: 1
direction: direction/reverse
top level: Rec-Open
start: 1838
end: 2560
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2296167
display id: annotation2296167
version: 1
top level: Rec-Open
location: https://synbiohub.org/public/igem/BBa_I726081/annotation2296167/range2296167/1
component: BBa_B0034

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type: Range
persistent identity: range2296167
display id: range2296167
version: 1
direction: direction/reverse
top level: Rec-Open
start: 64
end: 75
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2296177
display id: annotation2296177
version: 1
top level: Rec-Open
location: https://synbiohub.org/public/igem/BBa_I726081/annotation2296177/range2296177/1
component: BBa_B0010

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type: Range
persistent identity: range2296177
display id: range2296177
version: 1
direction: direction/forward
top level: Rec-Open
start: 1620
end: 1699
orientation: inline

BBa_R0062
type: SequenceAnnotation
persistent identity: annotation2296184
display id: annotation2296184
version: 1
top level: Rec-Open
location: https://synbiohub.org/public/igem/BBa_I726081/annotation2296184/range2296184/1
component: BBa_R0062

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type: Range
persistent identity: range2296184
display id: range2296184
version: 1
direction: direction/forward
top level: Rec-Open
start: 1757
end: 1811
orientation: inline

BBa_C0062
type: SequenceAnnotation
persistent identity: annotation2296170
display id: annotation2296170
version: 1
top level: Rec-Open
location: https://synbiohub.org/public/igem/BBa_I726081/annotation2296170/range2296170/1
component: BBa_C0062

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type: Range
persistent identity: range2296170
display id: range2296170
version: 1
direction: direction/forward
top level: Rec-Open
start: 82
end: 837
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2296191
display id: annotation2296191
version: 1
top level: Rec-Open
location: https://synbiohub.org/public/igem/BBa_I726081/annotation2296191/range2296191/1
component: BBa_B0010

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type: Range
persistent identity: range2296191
display id: range2296191
version: 1
direction: direction/forward
top level: Rec-Open
start: 2569
end: 2648
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2296175
display id: annotation2296175
version: 1
top level: Rec-Open
location: https://synbiohub.org/public/igem/BBa_I726081/annotation2296175/range2296175/1
component: BBa_B0034

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type: Range
persistent identity: range2296175
display id: range2296175
version: 1
direction: direction/reverse
top level: Rec-Open
start: 871
end: 882
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2296193
display id: annotation2296193
version: 1
top level: Rec-Open
location: https://synbiohub.org/public/igem/BBa_I726081/annotation2296193/range2296193/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I726081/annotation2296193/range2296193/1
type: Range
persistent identity: range2296193
display id: range2296193
version: 1
direction: direction/forward
top level: Rec-Open
start: 2657
end: 2697
orientation: inline

BBa_J37033
type: ComponentDefinition
persistent identity: BBa J37033
display id: BBa_J37033
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS + LuxR
creator: John Sy
created: 2006-08-22T11:00:00Z
modified: 2015-08-31T04:08:48Z
discontinued: false
dominant: false
experience: experience/None
group u list: _66_
m user id: 0
owner id: 1052
owning group id: 66
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: LuxR coding region with RBS
mutable notes: Designed for Imperial College iGEM 2006
mutable provenance: Ligation of B0034 + C0062
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_C0062
component: BBa_B0034
sequence annotation: BBa_C0062
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_J37033_sequence/1

BBa_C0062
type: Component
persistent identity: component2243719
display id: component2243719
version: 1
top level: BBa_J37033
access: public
definition: luxr

BBa_B0034
type: Component
persistent identity: component2243716
display id: component2243716
version: 1
top level: BBa_J37033
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_C0062
type: SequenceAnnotation
persistent identity: annotation2243719
display id: annotation2243719
version: 1
top level: BBa_J37033
location: https://synbiohub.org/public/igem/BBa_J37033/annotation2243719/range2243719/1
component: BBa_C0062

https://synbiohub.org/public/igem/BBa_J37033/annotation2243719/range2243719/1
type: Range
persistent identity: range2243719
display id: range2243719
version: 1
direction: direction/forward
top level: BBa_J37033
start: 19
end: 774
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2243716
display id: annotation2243716
version: 1
top level: BBa_J37033
location: https://synbiohub.org/public/igem/BBa_J37033/annotation2243716/range2243716/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_J37033/annotation2243716/range2243716/1
type: Range
persistent identity: range2243716
display id: range2243716
version: 1
direction: direction/reverse
top level: BBa_J37033
start: 1
end: 12
orientation: inline

BBa_I722012
type: ComponentDefinition
persistent identity: BBa I722012
display id: BBa_I722012
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: AHL->RoPs and mRFP producer
creator: Bo Hu
created: 2007-10-21T11:00:00Z
modified: 2015-08-31T04:07:53Z
discontinued: false
dominant: false
group u list: _150_
m user id: 0
owner id: 1325
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: accept AHL signal to produce RoPS and could also produce mRFP
mutable notes: could be easily connect to a protein coding region with a terminator
mutable provenance: ligation of original part
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0034
component: BBa_I722011
sequence annotation: BBa_B0034
sequence annotation: BBa_I722011
sequence: https://synbiohub.org/public/igem/BBa_I722012_sequence/1

BBa_B0034
type: Component
persistent identity: component2301823
display id: component2301823
version: 1
top level: BBa_I722012
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_I722011
type: Component
persistent identity: component2301821
display id: component2301821
version: 1
top level: BBa_I722012
access: public
definition: BBa_I722011

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2301823
display id: annotation2301823
version: 1
top level: BBa_I722012
location: https://synbiohub.org/public/igem/BBa_I722012/annotation2301823/range2301823/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I722012/annotation2301823/range2301823/1
type: Range
persistent identity: range2301823
display id: range2301823
version: 1
direction: direction/reverse
top level: BBa_I722012
start: 2711
end: 2722
orientation: inline

BBa_I722011
type: SequenceAnnotation
persistent identity: annotation2301821
display id: annotation2301821
version: 1
top level: BBa_I722012
location: https://synbiohub.org/public/igem/BBa_I722012/annotation2301821/range2301821/1
component: BBa_I722011

https://synbiohub.org/public/igem/BBa_I722012/annotation2301821/range2301821/1
type: Range
persistent identity: range2301821
display id: range2301821
version: 1
direction: direction/reverse
top level: BBa_I722012
start: 1
end: 2702
orientation: inline

BBa_C0261
type: ComponentDefinition
persistent identity: BBa C0261
display id: BBa_C0261
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: AHL-making Enzyme, luxI (+RBS)
creator: robertb
created: 2004-08-22T11:00:00Z
modified: 2015-08-31T04:07:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _6_
m user id: 0
owner id: 159
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: B0034.C0061
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_B0034
component: BBa_C0061
sequence annotation: BBa_C0061
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_C0261_sequence/1

BBa_B0034
type: Component
persistent identity: component1060403
display id: component1060403
version: 1
top level: BBa_C0261
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_C0061
type: Component
persistent identity: component1060413
display id: component1060413
version: 1
top level: BBa_C0261
access: public
definition: luxI

BBa_C0061
type: SequenceAnnotation
persistent identity: annotation1060413
display id: annotation1060413
version: 1
top level: BBa_C0261
location: https://synbiohub.org/public/igem/BBa_C0261/annotation1060413/range1060413/1
component: BBa_C0061

https://synbiohub.org/public/igem/BBa_C0261/annotation1060413/range1060413/1
type: Range
persistent identity: range1060413
display id: range1060413
version: 1
direction: direction/forward
top level: BBa_C0261
start: 19
end: 636
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1060403
display id: annotation1060403
version: 1
top level: BBa_C0261
location: https://synbiohub.org/public/igem/BBa_C0261/annotation1060403/range1060403/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_C0261/annotation1060403/range1060403/1
type: Range
persistent identity: range1060403
display id: range1060403
version: 1
direction: direction/forward
top level: BBa_C0261
start: 1
end: 12
orientation: inline

BBa_K1520511
type: ComponentDefinition
persistent identity: BBa K1520511
display id: BBa_K1520511
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: MC7-rbs-luxI-Ter
creator: yue wu
created: 2014-09-22T11:00:00Z
modified: 2015-05-08T01:10:49Z
discontinued: false
dominant: false
group u list: _1902_
m user id: 0
owner id: 21318
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: MC7-rbs-luxI-Ter If there are microcystin, the luxI will be produced.
mutable notes: None
mutable provenance: MC7, luxI, Ter
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1520029
component: BBa_K1520001
component: BBa_K1520504
sequence annotation: BBa_K1520504
sequence annotation: BBa_K1520001
sequence annotation: BBa_K1520029
sequence: https://synbiohub.org/public/igem/BBa_K1520511_sequence/1

BBa_K1520029
type: Component
persistent identity: component2385594
display id: component2385594
version: 1
top level: BBa_K1520511
access: public
definition: https://synbiohub.org/public/igem/BBa_K1520029/1

BBa_K1520001
type: Component
persistent identity: component2385590
display id: component2385590
version: 1
top level: BBa_K1520511
access: public
definition: https://synbiohub.org/public/igem/BBa_K1520001/1

BBa_K1520504
type: Component
persistent identity: component2385593
display id: component2385593
version: 1
top level: BBa_K1520511
access: public
definition: BBa_K1520504

BBa_K1520504
type: SequenceAnnotation
persistent identity: annotation2385593
display id: annotation2385593
version: 1
top level: BBa_K1520511
location: https://synbiohub.org/public/igem/BBa_K1520511/annotation2385593/range2385593/1
component: BBa_K1520504

https://synbiohub.org/public/igem/BBa_K1520511/annotation2385593/range2385593/1
type: Range
persistent identity: range2385593
display id: range2385593
version: 1
direction: direction/reverse
top level: BBa_K1520511
start: 142
end: 802
orientation: inline

BBa_K1520001
type: SequenceAnnotation
persistent identity: annotation2385590
display id: annotation2385590
version: 1
top level: BBa_K1520511
location: https://synbiohub.org/public/igem/BBa_K1520511/annotation2385590/range2385590/1
component: BBa_K1520001

https://synbiohub.org/public/igem/BBa_K1520511/annotation2385590/range2385590/1
type: Range
persistent identity: range2385590
display id: range2385590
version: 1
direction: direction/reverse
top level: BBa_K1520511
start: 1
end: 133
orientation: inline

BBa_K1520029
type: SequenceAnnotation
persistent identity: annotation2385594
display id: annotation2385594
version: 1
top level: BBa_K1520511
location: https://synbiohub.org/public/igem/BBa_K1520511/annotation2385594/range2385594/1
component: BBa_K1520029

https://synbiohub.org/public/igem/BBa_K1520511/annotation2385594/range2385594/1
type: Range
persistent identity: range2385594
display id: range2385594
version: 1
direction: direction/reverse
top level: BBa_K1520511
start: 811
end: 939
orientation: inline

luxr
type: ComponentDefinition
persistent identity: BBa C0062
display id: BBa_C0062
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: luxR repressor/activator, (no LVA?)
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:23Z
discontinued: false
dominant: true
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: In complex with HSL, LuxR binds to the Lux promoter, activating transcription from Pr <bb_part>BBa_R0062</bb_part>, and repressing transcription from Pl <bb_part>BBa_R0063</bb_part>. The lux cassette of V. fischeri contains a left and a right promoter. The right promoter gives weak constitutive expression of downstream genes.This expression is up-regulated by the action of the Lux activator, LuxR complexed to HSL. Two molecules of LuxR protein form a complex with two molecules the signalling compound homoserine lactone (HSL). This complex binds to a palindromic site on the promoter, increasing the rate of transcription.
mutable notes: 2 silent point mutants were introduced in the coding sequence to remove internal XbaI and PstI sites. Mutation sites were chosen to replace codons commonly used in E. coli with codons used at a similar frequency.
mutable provenance: V. fischeri AF170104
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: luxR
sequence annotation: prefix
sequence annotation: Help:Barcodes
sequence annotation: BBa_C0062
sequence annotation: T
sequence annotation: A
sequence: https://synbiohub.org/public/igem/BBa_C0062_sequence/1

luxR
type: SequenceAnnotation
persistent identity: annotation1766
display id: annotation1766
version: 1
top level: luxr
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_C0062/annotation1766/range1766/1

https://synbiohub.org/public/igem/BBa_C0062/annotation1766/range1766/1
type: Range
persistent identity: range1766
display id: range1766
version: 1
direction: direction/forward
top level: luxr
start: 1
end: 750
orientation: inline

prefix
type: SequenceAnnotation
persistent identity: annotation1762
display id: annotation1762
version: 1
top level: luxr
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_C0062/annotation1762/range1762/1

https://synbiohub.org/public/igem/BBa_C0062/annotation1762/range1762/1
type: Range
persistent identity: range1762
display id: range1762
version: 1
direction: direction/forward
top level: luxr
start: 1
end: 2
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213986
display id: annotation2213986
version: 1
top level: luxr
role: SO:0000807
role: feature/barcode
location: https://synbiohub.org/public/igem/BBa_C0062/annotation2213986/range2213986/1

https://synbiohub.org/public/igem/BBa_C0062/annotation2213986/range2213986/1
type: Range
persistent identity: range2213986
display id: range2213986
version: 1
direction: direction/forward
top level: luxr
start: 757
end: 781
orientation: inline

BBa_C0062
type: SequenceAnnotation
persistent identity: annotation7039
display id: annotation7039
version: 1
top level: luxr
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_C0062/annotation7039/range7039/1

https://synbiohub.org/public/igem/BBa_C0062/annotation7039/range7039/1
type: Range
persistent identity: range7039
display id: range7039
version: 1
direction: direction/forward
top level: luxr
start: 1
end: 756
orientation: inline

T
type: SequenceAnnotation
persistent identity: annotation1764
display id: annotation1764
version: 1
top level: luxr
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_C0062/annotation1764/range1764/1

https://synbiohub.org/public/igem/BBa_C0062/annotation1764/range1764/1
type: Range
persistent identity: range1764
display id: range1764
version: 1
direction: direction/forward
top level: luxr
start: 174
end: 174
orientation: inline

A
type: SequenceAnnotation
persistent identity: annotation1765
display id: annotation1765
version: 1
top level: luxr
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_C0062/annotation1765/range1765/1

https://synbiohub.org/public/igem/BBa_C0062/annotation1765/range1765/1
type: Range
persistent identity: range1765
display id: range1765
version: 1
direction: direction/forward
top level: luxr
start: 492
end: 492
orientation: inline

BBa_I751350
type: ComponentDefinition
persistent identity: BBa I751350
display id: BBa_I751350
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pBR322 plac-luxI
creator: Kenichiro Iwasaki
created: 2007-10-22T11:00:00Z
modified: 2015-08-31T04:08:06Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _151_
m user id: 0
owner id: 2188
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0034
component: BBa_B0010
component: BBa_C0161
component: BBa_B0012
component: BBa_R0011
sequence annotation: BBa_B0034
sequence annotation: BBa_B0010
sequence annotation: BBa_R0011
sequence annotation: BBa_C0161
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_I751350_sequence/1

BBa_B0034
type: Component
persistent identity: component1953076
display id: component1953076
version: 1
top level: BBa_I751350
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0010
type: Component
persistent identity: component1953079
display id: component1953079
version: 1
top level: BBa_I751350
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_C0161
type: Component
persistent identity: component1953078
display id: component1953078
version: 1
top level: BBa_I751350
access: public
definition: luxI

BBa_B0012
type: Component
persistent identity: component1953081
display id: component1953081
version: 1
top level: BBa_I751350
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_R0011
type: Component
persistent identity: component1953070
display id: component1953070
version: 1
top level: BBa_I751350
access: public
definition: https://synbiohub.org/public/igem/BBa_R0011/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1953076
display id: annotation1953076
version: 1
top level: BBa_I751350
location: https://synbiohub.org/public/igem/BBa_I751350/annotation1953076/range1953076/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I751350/annotation1953076/range1953076/1
type: Range
persistent identity: range1953076
display id: range1953076
version: 1
direction: direction/reverse
top level: BBa_I751350
start: 64
end: 75
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1953079
display id: annotation1953079
version: 1
top level: BBa_I751350
location: https://synbiohub.org/public/igem/BBa_I751350/annotation1953079/range1953079/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I751350/annotation1953079/range1953079/1
type: Range
persistent identity: range1953079
display id: range1953079
version: 1
direction: direction/forward
top level: BBa_I751350
start: 675
end: 754
orientation: inline

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation1953070
display id: annotation1953070
version: 1
top level: BBa_I751350
location: https://synbiohub.org/public/igem/BBa_I751350/annotation1953070/range1953070/1
component: BBa_R0011

https://synbiohub.org/public/igem/BBa_I751350/annotation1953070/range1953070/1
type: Range
persistent identity: range1953070
display id: range1953070
version: 1
direction: direction/forward
top level: BBa_I751350
start: 1
end: 54
orientation: inline

BBa_C0161
type: SequenceAnnotation
persistent identity: annotation1953078
display id: annotation1953078
version: 1
top level: BBa_I751350
location: https://synbiohub.org/public/igem/BBa_I751350/annotation1953078/range1953078/1
component: BBa_C0161

https://synbiohub.org/public/igem/BBa_I751350/annotation1953078/range1953078/1
type: Range
persistent identity: range1953078
display id: range1953078
version: 1
direction: direction/reverse
top level: BBa_I751350
start: 82
end: 666
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1953081
display id: annotation1953081
version: 1
top level: BBa_I751350
location: https://synbiohub.org/public/igem/BBa_I751350/annotation1953081/range1953081/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I751350/annotation1953081/range1953081/1
type: Range
persistent identity: range1953081
display id: range1953081
version: 1
direction: direction/forward
top level: BBa_I751350
start: 763
end: 803
orientation: inline

BBa_K819005
type: ComponentDefinition
persistent identity: BBa K819005
display id: BBa_K819005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Constitutive LuxBrick Generator
creator: ZHANG Zidong
created: 2012-09-01T11:00:00Z
modified: 2015-05-08T01:13:29Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _1077_
m user id: 0
owner id: 13785
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: a fusion protein consisting of E.coli endogenous SOS system repressor LexA and fungus N.crass photosensor protein VVD with LexA carrying mutation at position 40-42 and VVD carrying mutation N56K, C71V and I135M. This part serves as an ultra sensitive photoreceptor(which can sense light as weak as moonlight) and will induce a light-dependent repression of genes with their promoter containing mutated 408 form of SOS box.
mutable notes: LexA carrying mutation at position 40-42 and VVD carrying mutation N56K, C71V and I135M enable the sensor recognize 408 form of SOS box and with enhanced performance.
mutable provenance: Construct by Peking2012.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: LuxCDABEG from K325909
sequence annotation: BBa_K819008
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_K819005_sequence/1

LuxCDABEG from K325909
type: SequenceAnnotation
persistent identity: annotation2181624
display id: annotation2181624
version: 1
top level: BBa_K819005
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K819005/annotation2181624/range2181624/1

https://synbiohub.org/public/igem/BBa_K819005/annotation2181624/range2181624/1
type: Range
persistent identity: range2181624
display id: range2181624
version: 1
direction: direction/forward
top level: BBa_K819005
start: 53
end: 6477
orientation: inline

BBa_K819008
type: SequenceAnnotation
persistent identity: annotation2181623
display id: annotation2181623
version: 1
top level: BBa_K819005
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K819005/annotation2181623/range2181623/1

https://synbiohub.org/public/igem/BBa_K819005/annotation2181623/range2181623/1
type: Range
persistent identity: range2181623
display id: range2181623
version: 1
direction: direction/forward
top level: BBa_K819005
start: 1
end: 46
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2212111
display id: annotation2212111
version: 1
top level: BBa_K819005
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K819005/annotation2212111/range2212111/1

https://synbiohub.org/public/igem/BBa_K819005/annotation2212111/range2212111/1
type: Range
persistent identity: range2212111
display id: range2212111
version: 1
direction: direction/forward
top level: BBa_K819005
start: 6478
end: 6480
orientation: inline

BBa_I13266
type: ComponentDefinition
persistent identity: BBa I13266
display id: BBa_I13266
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Lux-based Sender/Receiver Device which outputs PoPS
creator: Barry Canton
created: 2004-08-08T11:00:00Z
modified: 2015-08-31T04:07:33Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_6_
m user id: 0
owner id: 135
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: BioBricked version of Natural Lux system but has no terminator - allows addition of cis elements
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Signalling
role: SO:0000804
component: BBa_B0010
component: BBa_R0063
component: BBa_C0062
component: BBa_R0062
component: BBa_B0012
component: BBa_C0061
component: BBa_B0034
sequence annotation: BBa_B0034
sequence annotation: BBa_R0063
sequence annotation: BBa_C0062
sequence annotation: BBa_C0061
sequence annotation: BBa_R0062
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_I13266_sequence/1

BBa_B0010
type: Component
persistent identity: component1038542
display id: component1038542
version: 1
top level: BBa_I13266
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_R0063
type: Component
persistent identity: component1038510
display id: component1038510
version: 1
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BBa_C0062
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BBa_R0062
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BBa_C0061
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BBa_B0034
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BBa_K091138
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display id: BBa_K091138
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS+lsrR
creator: Xiao Zhu
created: 2008-07-14T11:00:00Z
modified: 2015-05-08T01:08:38Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _191_
m user id: 0
owner id: 3130
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: lsrR coding gene with ribosome binding site B0034.
mutable notes: no.
mutable provenance: lsrR comes from E. coli MG1655
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owned by: myers
star: false
type: DnaRegion
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BBa_B0034
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BBa_K1072017
type: ComponentDefinition
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display id: BBa_K1072017
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pGAL1 + Flag + odr-10 + GFP + ADH1Te + pFUS1 + BFP + ADH1Te
creator: Junqi kuang
created: 2013-09-20T11:00:00Z
modified: 2015-05-08T01:09:01Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1381_
m user id: 0
owner id: 16002
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: -
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
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role: partType/Composite
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component: BBa_K1072013
sequence annotation: BBa_K1072013
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was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: 3OC6HSL Receiver Device with Inducible Control of LuxR and a YFP Output device
creator: CC Signalling
created: 2004-07-19T11:00:00Z
modified: 2015-08-31T04:07:32Z
discontinued: false
dominant: false
experience: experience/None
group u list: _6_
m user id: 0
owner id: 101
owning group id: 7
sample status: Not in stock
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bookmark: false
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star: false
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component: BBa_B0034
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BBa_C0062
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BBa_B0010
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BBa_B0034
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BBa_B0034
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BBa_R0010
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BBa_C0040
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BBa_B0034
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BBa_R0010
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end: 2155
orientation: inline

BBa_I534060
type: ComponentDefinition
persistent identity: BBa I534060
display id: BBa_I534060
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: aiiA Protein Generator (LVA+) Controlled by a tet Repressible Promoter
creator: Barry Canton
created: 2004-07-29T11:00:00Z
modified: 2015-08-31T04:07:42Z
discontinued: false
dominant: true
experience: experience/None
group u list: _11_6_
m user id: 0
owner id: 135
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Outputs varying levels of aiiA as part of HSL/aiiA measurements. Subpart of I13205 Formerly known as I13204
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Signalling
role: SO:0000804
component: BBa_B0010
component: BBa_B0034
component: BBa_I0500
component: BBa_B0012
component: BBa_C0060
sequence annotation: BBa_I0500
sequence annotation: BBa_B0012
sequence annotation: BBa_C0060
sequence annotation: BBa_B0034
sequence annotation: BBa_B0010
sequence: https://synbiohub.org/public/igem/BBa_I534060_sequence/1

BBa_B0010
type: Component
persistent identity: component1016227
display id: component1016227
version: 1
top level: BBa_I534060
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0034
type: Component
persistent identity: component1016204
display id: component1016204
version: 1
top level: BBa_I534060
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_I0500
type: Component
persistent identity: component1016199
display id: component1016199
version: 1
top level: BBa_I534060
access: public
definition: https://synbiohub.org/public/igem/BBa_I0500/1

BBa_B0012
type: Component
persistent identity: component1016237
display id: component1016237
version: 1
top level: BBa_I534060
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_C0060
type: Component
persistent identity: component1016219
display id: component1016219
version: 1
top level: BBa_I534060
access: public
definition: aiiA

BBa_I0500
type: SequenceAnnotation
persistent identity: annotation1016199
display id: annotation1016199
version: 1
top level: BBa_I534060
location: https://synbiohub.org/public/igem/BBa_I534060/annotation1016199/range1016199/1
component: BBa_I0500

https://synbiohub.org/public/igem/BBa_I534060/annotation1016199/range1016199/1
type: Range
persistent identity: range1016199
display id: range1016199
version: 1
direction: direction/forward
top level: BBa_I534060
start: 1
end: 1210
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1016237
display id: annotation1016237
version: 1
top level: BBa_I534060
location: https://synbiohub.org/public/igem/BBa_I534060/annotation1016237/range1016237/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I534060/annotation1016237/range1016237/1
type: Range
persistent identity: range1016237
display id: range1016237
version: 1
direction: direction/forward
top level: BBa_I534060
start: 2147
end: 2187
orientation: inline

BBa_C0060
type: SequenceAnnotation
persistent identity: annotation1016219
display id: annotation1016219
version: 1
top level: BBa_I534060
location: https://synbiohub.org/public/igem/BBa_I534060/annotation1016219/range1016219/1
component: BBa_C0060

https://synbiohub.org/public/igem/BBa_I534060/annotation1016219/range1016219/1
type: Range
persistent identity: range1016219
display id: range1016219
version: 1
direction: direction/forward
top level: BBa_I534060
start: 1237
end: 2025
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1016204
display id: annotation1016204
version: 1
top level: BBa_I534060
location: https://synbiohub.org/public/igem/BBa_I534060/annotation1016204/range1016204/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I534060/annotation1016204/range1016204/1
type: Range
persistent identity: range1016204
display id: range1016204
version: 1
direction: direction/forward
top level: BBa_I534060
start: 1219
end: 1230
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1016227
display id: annotation1016227
version: 1
top level: BBa_I534060
location: https://synbiohub.org/public/igem/BBa_I534060/annotation1016227/range1016227/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I534060/annotation1016227/range1016227/1
type: Range
persistent identity: range1016227
display id: range1016227
version: 1
direction: direction/forward
top level: BBa_I534060
start: 2059
end: 2138
orientation: inline

BBa_K091109
type: ComponentDefinition
persistent identity: BBa K091109
display id: BBa_K091109
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LuxS
creator: Andrew Gordon
created: 2008-06-18T11:00:00Z
modified: 2015-05-08T01:08:37Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _191_
m user id: 0
owner id: 3129
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: LuxS is a synthase for the AI-2 quorum sensing system. This protein is involved in the production of small molecules that are readily absorbed by multiple species of bacteria. LuxS can be used as part of a positive feedback loop in cell to cell signaling.
mutable notes: Nucleotide position 54 changed from A to T in order to destroy a PstI site; this is a sense mutation Also changed stop codon from TAG to TAA (recommended in Registry Help)
mutable provenance: The LuxS sequence was pulled from E. coli MG1655 by predesigned primers.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K091109_sequence/1

rhII
type: ComponentDefinition
persistent identity: BBa C0070
display id: BBa_C0070
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: autoinducer synthetase for N-butyryl-HSL (BHL) and HHL
creator: Debra Lin, Srini Devadas, David Gray, Ronny Krashinsky, and Chris Zheng Liu, Boopers
created: 2004-01-26T12:00:00Z
modified: 2015-08-31T04:07:23Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Autoinducer synthesis protein that produces N-butyryl-HSL which binds to RhlR, obtained from Pseudomonas aeruginosa.
mutable notes: Editing Check (1/28/04) (0) BB sites cleaned (1) ATG (2) TAATAA (3) Blast checks out (i.e., it's RhlI) (4) Codon changes OK (AA and tRNA usage) (5) Chassis might have conflict (w/ native autoinducer system?) (6) ssrA tag added (7) barcode added (8) Codon optimized for expression in enteric bacteria 2 silent point mutations have been included into the sequence at base 138 from A to G and at base 576 from G to C to remove internal EcoRI and PstI sites. Mutations were chosen to yield the same amino acid and codons of similar frequency in E. coli.
mutable provenance: P. aeruginosa PA3476
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: rhlL
sequence annotation: LVA
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0070_sequence/1

rhlL
type: SequenceAnnotation
persistent identity: annotation301203
display id: annotation301203
version: 1
top level: rhII
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_C0070/annotation301203/range301203/1

https://synbiohub.org/public/igem/BBa_C0070/annotation301203/range301203/1
type: Range
persistent identity: range301203
display id: range301203
version: 1
direction: direction/forward
top level: rhII
start: 1
end: 603
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation306820
display id: annotation306820
version: 1
top level: rhII
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_C0070/annotation306820/range306820/1

https://synbiohub.org/public/igem/BBa_C0070/annotation306820/range306820/1
type: Range
persistent identity: range306820
display id: range306820
version: 1
direction: direction/forward
top level: rhII
start: 604
end: 636
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213996
display id: annotation2213996
version: 1
top level: rhII
role: SO:0000807
role: feature/barcode
location: https://synbiohub.org/public/igem/BBa_C0070/annotation2213996/range2213996/1

https://synbiohub.org/public/igem/BBa_C0070/annotation2213996/range2213996/1
type: Range
persistent identity: range2213996
display id: range2213996
version: 1
direction: direction/forward
top level: rhII
start: 643
end: 667
orientation: inline

BBa_K082021
type: ComponentDefinition
persistent identity: BBa K082021
display id: BBa_K082021
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RhlI generator
creator: Zhen Xu , Binjie Xu
created: 2008-10-28T12:00:00Z
modified: 2015-05-08T01:08:35Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _228_
m user id: 0
owner id: 1560
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: none
mutable notes: none
mutable provenance: none
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_I0466
component: BBa_R0040
sequence annotation: BBa_I0466
sequence annotation: BBa_R0040
sequence: https://synbiohub.org/public/igem/BBa_K082021_sequence/1

BBa_I0466
type: Component
persistent identity: component2254855
display id: component2254855
version: 1
top level: BBa_K082021
access: public
definition: BBa_I0466

BBa_R0040
type: Component
persistent identity: component2254838
display id: component2254838
version: 1
top level: BBa_K082021
access: public
definition: https://synbiohub.org/public/igem/BBa_R0040/1

BBa_I0466
type: SequenceAnnotation
persistent identity: annotation2254855
display id: annotation2254855
version: 1
top level: BBa_K082021
location: https://synbiohub.org/public/igem/BBa_K082021/annotation2254855/range2254855/1
component: BBa_I0466

https://synbiohub.org/public/igem/BBa_K082021/annotation2254855/range2254855/1
type: Range
persistent identity: range2254855
display id: range2254855
version: 1
direction: direction/reverse
top level: BBa_K082021
start: 63
end: 1004
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2254838
display id: annotation2254838
version: 1
top level: BBa_K082021
location: https://synbiohub.org/public/igem/BBa_K082021/annotation2254838/range2254838/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_K082021/annotation2254838/range2254838/1
type: Range
persistent identity: range2254838
display id: range2254838
version: 1
direction: direction/forward
top level: BBa_K082021
start: 1
end: 54
orientation: inline

BBa_K082029
type: ComponentDefinition
persistent identity: BBa K082029
display id: BBa_K082029
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LuxI generator
creator: Yanting Xue , He Shen
created: 2008-10-28T12:00:00Z
modified: 2015-05-08T01:08:36Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _228_
m user id: 0
owner id: 1560
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: none
mutable notes: none
mutable provenance: none
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_R0040
component: BBa_C0261
component: BBa_B0015
sequence annotation: BBa_C0261
sequence annotation: BBa_R0040
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_K082029_sequence/1

BBa_R0040
type: Component
persistent identity: component2254819
display id: component2254819
version: 1
top level: BBa_K082029
access: public
definition: https://synbiohub.org/public/igem/BBa_R0040/1

BBa_C0261
type: Component
persistent identity: component2254830
display id: component2254830
version: 1
top level: BBa_K082029
access: public
definition: BBa_C0261

BBa_B0015
type: Component
persistent identity: component2254837
display id: component2254837
version: 1
top level: BBa_K082029
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_C0261
type: SequenceAnnotation
persistent identity: annotation2254830
display id: annotation2254830
version: 1
top level: BBa_K082029
location: https://synbiohub.org/public/igem/BBa_K082029/annotation2254830/range2254830/1
component: BBa_C0261

https://synbiohub.org/public/igem/BBa_K082029/annotation2254830/range2254830/1
type: Range
persistent identity: range2254830
display id: range2254830
version: 1
direction: direction/reverse
top level: BBa_K082029
start: 63
end: 723
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2254819
display id: annotation2254819
version: 1
top level: BBa_K082029
location: https://synbiohub.org/public/igem/BBa_K082029/annotation2254819/range2254819/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_K082029/annotation2254819/range2254819/1
type: Range
persistent identity: range2254819
display id: range2254819
version: 1
direction: direction/forward
top level: BBa_K082029
start: 1
end: 54
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2254837
display id: annotation2254837
version: 1
top level: BBa_K082029
location: https://synbiohub.org/public/igem/BBa_K082029/annotation2254837/range2254837/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K082029/annotation2254837/range2254837/1
type: Range
persistent identity: range2254837
display id: range2254837
version: 1
direction: direction/reverse
top level: BBa_K082029
start: 732
end: 860
orientation: inline

BBa_I13210
type: ComponentDefinition
persistent identity: BBa I13210
display id: BBa_I13210
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Lux/Cin Relaxation Oscillator
creator: SMUG Cell-Cell Signalling: Jay
created: 2004-07-29T11:00:00Z
modified: 2015-08-31T04:07:33Z
discontinued: false
dominant: false
experience: experience/None
group u list: _6_
m user id: 0
owner id: 101
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Reporter tripped by CinR-HSL Threshold, tripped by Lux-HSL Threshold. CinR-HSL also promotes production of aiiA. This should break the thresholds and reset the system.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Signalling
role: SO:0000804
component: BBa_C0077
component: BBa_I13201
component: BBa_F1760
component: BBa_E0420
component: BBa_R0078
component: BBa_C0061
component: BBa_B0034
component: BBa_I0460
sequence annotation: BBa_E0420
sequence annotation: BBa_C0077
sequence annotation: BBa_B0034
sequence annotation: BBa_I13201
sequence annotation: BBa_I0460
sequence annotation: BBa_R0078
sequence annotation: BBa_C0061
sequence annotation: BBa_F1760
sequence: https://synbiohub.org/public/igem/BBa_I13210_sequence/1

BBa_C0077
type: Component
persistent identity: component2237186
display id: component2237186
version: 1
top level: BBa_I13210
access: public
definition: cinr

BBa_I13201
type: Component
persistent identity: component2237174
display id: component2237174
version: 1
top level: BBa_I13210
access: public
definition: https://synbiohub.org/public/igem/BBa_I13201/1

BBa_F1760
type: Component
persistent identity: component2237199
display id: component2237199
version: 1
top level: BBa_I13210
access: public
definition: https://synbiohub.org/public/igem/BBa_F1760/1

BBa_E0420
type: Component
persistent identity: component2237173
display id: component2237173
version: 1
top level: BBa_I13210
access: public
definition: https://synbiohub.org/public/igem/BBa_E0420/1

BBa_R0078
type: Component
persistent identity: component2237163
display id: component2237163
version: 1
top level: BBa_I13210
access: public
definition: https://synbiohub.org/public/igem/BBa_R0078/1

BBa_C0061
type: Component
persistent identity: component2237178
display id: component2237178
version: 1
top level: BBa_I13210
access: public
definition: luxI

BBa_B0034
type: Component
persistent identity: component2237176
display id: component2237176
version: 1
top level: BBa_I13210
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_R0078
type: Component
persistent identity: component2237144
display id: component2237144
version: 1
top level: BBa_I13210
access: public
definition: https://synbiohub.org/public/igem/BBa_R0078/1

BBa_B0034
type: Component
persistent identity: component2237182
display id: component2237182
version: 1
top level: BBa_I13210
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_I0460
type: Component
persistent identity: component2237159
display id: component2237159
version: 1
top level: BBa_I13210
access: public
definition: BBa_I0460

BBa_E0420
type: SequenceAnnotation
persistent identity: annotation2237173
display id: annotation2237173
version: 1
top level: BBa_I13210
location: https://synbiohub.org/public/igem/BBa_I13210/annotation2237173/range2237173/1
component: BBa_E0420

https://synbiohub.org/public/igem/BBa_I13210/annotation2237173/range2237173/1
type: Range
persistent identity: range2237173
display id: range2237173
version: 1
direction: direction/reverse
top level: BBa_I13210
start: 1444
end: 2321
orientation: inline

BBa_C0077
type: SequenceAnnotation
persistent identity: annotation2237186
display id: annotation2237186
version: 1
top level: BBa_I13210
location: https://synbiohub.org/public/igem/BBa_I13210/annotation2237186/range2237186/1
component: BBa_C0077

https://synbiohub.org/public/igem/BBa_I13210/annotation2237186/range2237186/1
type: Range
persistent identity: range2237186
display id: range2237186
version: 1
direction: direction/reverse
top level: BBa_I13210
start: 4048
end: 4834
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2237182
display id: annotation2237182
version: 1
top level: BBa_I13210
location: https://synbiohub.org/public/igem/BBa_I13210/annotation2237182/range2237182/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I13210/annotation2237182/range2237182/1
type: Range
persistent identity: range2237182
display id: range2237182
version: 1
direction: direction/reverse
top level: BBa_I13210
start: 4030
end: 4041
orientation: inline

BBa_I13201
type: SequenceAnnotation
persistent identity: annotation2237174
display id: annotation2237174
version: 1
top level: BBa_I13210
location: https://synbiohub.org/public/igem/BBa_I13210/annotation2237174/range2237174/1
component: BBa_I13201

https://synbiohub.org/public/igem/BBa_I13210/annotation2237174/range2237174/1
type: Range
persistent identity: range2237174
display id: range2237174
version: 1
direction: direction/reverse
top level: BBa_I13210
start: 2330
end: 3352
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2237176
display id: annotation2237176
version: 1
top level: BBa_I13210
location: https://synbiohub.org/public/igem/BBa_I13210/annotation2237176/range2237176/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I13210/annotation2237176/range2237176/1
type: Range
persistent identity: range2237176
display id: range2237176
version: 1
direction: direction/reverse
top level: BBa_I13210
start: 3361
end: 3372
orientation: inline

BBa_I0460
type: SequenceAnnotation
persistent identity: annotation2237159
display id: annotation2237159
version: 1
top level: BBa_I13210
location: https://synbiohub.org/public/igem/BBa_I13210/annotation2237159/range2237159/1
component: BBa_I0460

https://synbiohub.org/public/igem/BBa_I13210/annotation2237159/range2237159/1
type: Range
persistent identity: range2237159
display id: range2237159
version: 1
direction: direction/reverse
top level: BBa_I13210
start: 234
end: 1202
orientation: inline

BBa_R0078
type: SequenceAnnotation
persistent identity: annotation2237144
display id: annotation2237144
version: 1
top level: BBa_I13210
location: https://synbiohub.org/public/igem/BBa_I13210/annotation2237144/range2237144/1
component: BBa_R0078

https://synbiohub.org/public/igem/BBa_I13210/annotation2237144/range2237144/1
type: Range
persistent identity: range2237144
display id: range2237144
version: 1
direction: direction/reverse
top level: BBa_I13210
start: 1
end: 225
orientation: inline

BBa_C0061
type: SequenceAnnotation
persistent identity: annotation2237178
display id: annotation2237178
version: 1
top level: BBa_I13210
location: https://synbiohub.org/public/igem/BBa_I13210/annotation2237178/range2237178/1
component: BBa_C0061

https://synbiohub.org/public/igem/BBa_I13210/annotation2237178/range2237178/1
type: Range
persistent identity: range2237178
display id: range2237178
version: 1
direction: direction/forward
top level: BBa_I13210
start: 3379
end: 3996
orientation: inline

BBa_R0078
type: SequenceAnnotation
persistent identity: annotation2237163
display id: annotation2237163
version: 1
top level: BBa_I13210
location: https://synbiohub.org/public/igem/BBa_I13210/annotation2237163/range2237163/1
component: BBa_R0078

https://synbiohub.org/public/igem/BBa_I13210/annotation2237163/range2237163/1
type: Range
persistent identity: range2237163
display id: range2237163
version: 1
direction: direction/reverse
top level: BBa_I13210
start: 1211
end: 1435
orientation: inline

BBa_F1760
type: SequenceAnnotation
persistent identity: annotation2237199
display id: annotation2237199
version: 1
top level: BBa_I13210
location: https://synbiohub.org/public/igem/BBa_I13210/annotation2237199/range2237199/1
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display id: BBa_I1466
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RhlR protein generator (LVA-)
creator: Barry Canton
created: 2004-08-09T11:00:00Z
modified: 2015-08-31T04:07:38Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_6_
m user id: 0
owner id: 135
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Elowitz RBS. RhlR CDS (no LVA tag) and terminator. Same as I0466 but no LVA tag
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owned by: myers
star: true
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role: SO:0000804
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component: BBa_B0034
component: BBa_B0012
component: BBa_C0171
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definition: rhIR

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description: Lux pL controlled LuxR + lux pR autoinducing LuxI (lva tag) + lux pR controlled GFP(lva tag).
creator: Matthijn Hesselman
created: 2011-09-17T11:00:00Z
modified: 2015-05-08T01:12:39Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _713_
m user id: 0
owner id: 8603
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status: status/Planning
bookmark: true
mutable description: in progress
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mutable provenance: in progress
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owned by: myers
star: false
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BBa_I0424
type: ComponentDefinition
persistent identity: BBa I0424
display id: BBa_I0424
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: I0404.I6101
creator: jcbraff
created: 2004-07-13T11:00:00Z
modified: 2015-08-31T04:07:29Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_1_
m user id: 0
owner id: 61
owning group id: 7
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: I0404.I6101 Rhl test
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Signalling
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definition: https://synbiohub.org/public/igem/BBa_R0071/1

BBa_B0010
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definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_R0040
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BBa_B0034
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BBa_E0020
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definition: https://synbiohub.org/public/igem/BBa_E0020/1

BBa_B0012
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definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_C0071
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definition: rhlR

BBa_B0012
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definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0034
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BBa_C0071
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BBa_E0020
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BBa_B0012
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BBa_R0040
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BBa_B0010
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BBa_B0034
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BBa_E0030
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BBa_B0034
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BBa_B0010
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BBa_R0071
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version: 1
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location: https://synbiohub.org/public/igem/BBa_I0424/annotation973779/range973779/1
component: BBa_R0071

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BBa_I1468
type: ComponentDefinition
persistent identity: BBa I1468
display id: BBa_I1468
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CinR protein generator
creator: Barry Canton
created: 2004-08-09T11:00:00Z
modified: 2015-08-31T04:07:38Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_6_
m user id: 0
owner id: 135
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Elowitz RBS. CinR CDS (no LVA tag) and terminator. Same as I0468 but no LVA tag
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0034
component: BBa_B0010
component: BBa_C0177
component: BBa_B0012
sequence annotation: BBa_B0012
sequence annotation: BBa_B0034
sequence annotation: BBa_C0177
sequence annotation: BBa_B0010
sequence: https://synbiohub.org/public/igem/BBa_I1468_sequence/1

BBa_B0034
type: Component
persistent identity: component1040158
display id: component1040158
version: 1
top level: BBa_I1468
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0010
type: Component
persistent identity: component1040165
display id: component1040165
version: 1
top level: BBa_I1468
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_C0177
type: Component
persistent identity: component1040160
display id: component1040160
version: 1
top level: BBa_I1468
access: public
definition: https://synbiohub.org/public/igem/BBa_C0177/1

BBa_B0012
type: Component
persistent identity: component1040175
display id: component1040175
version: 1
top level: BBa_I1468
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1040175
display id: annotation1040175
version: 1
top level: BBa_I1468
location: https://synbiohub.org/public/igem/BBa_I1468/annotation1040175/range1040175/1
component: BBa_B0012

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display id: range1040175
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orientation: inline

BBa_B0034
type: SequenceAnnotation
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display id: annotation1040158
version: 1
top level: BBa_I1468
location: https://synbiohub.org/public/igem/BBa_I1468/annotation1040158/range1040158/1
component: BBa_B0034

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persistent identity: range1040158
display id: range1040158
version: 1
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orientation: inline

BBa_C0177
type: SequenceAnnotation
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display id: annotation1040160
version: 1
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component: BBa_C0177

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persistent identity: range1040160
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direction: direction/forward
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end: 747
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1040165
display id: annotation1040165
version: 1
top level: BBa_I1468
location: https://synbiohub.org/public/igem/BBa_I1468/annotation1040165/range1040165/1
component: BBa_B0010

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persistent identity: range1040165
display id: range1040165
version: 1
direction: direction/forward
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start: 756
end: 835
orientation: inline

BBa_I13213
type: ComponentDefinition
persistent identity: BBa I13213
display id: BBa_I13213
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: BioBricked version of the natural Lux system with order reversed
creator: Barry Canton
created: 2004-08-04T11:00:00Z
modified: 2015-08-31T04:07:33Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_6_
m user id: 0
owner id: 135
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Places both LuxI and LuxR under the control of their natural promoters but reverses the order relative to I13211
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Signalling
role: SO:0000804
component: BBa_C0061
component: BBa_B0012
component: BBa_B0010
component: BBa_C0062
component: BBa_B0034
component: BBa_R0063
component: BBa_R0062
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_C0062
sequence annotation: BBa_R0062
sequence annotation: BBa_C0061
sequence annotation: BBa_B0034
sequence annotation: BBa_R0063
sequence: https://synbiohub.org/public/igem/BBa_I13213_sequence/1

BBa_C0061
type: Component
persistent identity: component1030555
display id: component1030555
version: 1
top level: BBa_I13213
access: public
definition: luxI

BBa_B0012
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persistent identity: component1030572
display id: component1030572
version: 1
top level: BBa_I13213
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0010
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persistent identity: component1030562
display id: component1030562
version: 1
top level: BBa_I13213
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_C0062
type: Component
persistent identity: component1030613
display id: component1030613
version: 1
top level: BBa_I13213
access: public
definition: luxr

BBa_B0034
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persistent identity: component1030545
display id: component1030545
version: 1
top level: BBa_I13213
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0010
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persistent identity: component1030621
display id: component1030621
version: 1
top level: BBa_I13213
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_R0063
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display id: component1030589
version: 1
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access: public
definition: https://synbiohub.org/public/igem/BBa_R0063/1

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display id: component1030537
version: 1
top level: BBa_I13213
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definition: https://synbiohub.org/public/igem/BBa_R0062/1

BBa_B0012
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display id: component1030631
version: 1
top level: BBa_I13213
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0034
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display id: component1030598
version: 1
top level: BBa_I13213
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1030621
display id: annotation1030621
version: 1
top level: BBa_I13213
location: https://synbiohub.org/public/igem/BBa_I13213/annotation1030621/range1030621/1
component: BBa_B0010

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direction: direction/forward
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end: 1915
orientation: inline

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type: SequenceAnnotation
persistent identity: annotation1030631
display id: annotation1030631
version: 1
top level: BBa_I13213
location: https://synbiohub.org/public/igem/BBa_I13213/annotation1030631/range1030631/1
component: BBa_B0012

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persistent identity: range1030631
display id: range1030631
version: 1
direction: direction/forward
top level: BBa_I13213
start: 1924
end: 1964
orientation: inline

BBa_C0062
type: SequenceAnnotation
persistent identity: annotation1030613
display id: annotation1030613
version: 1
top level: BBa_I13213
location: https://synbiohub.org/public/igem/BBa_I13213/annotation1030613/range1030613/1
component: BBa_C0062

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persistent identity: range1030613
display id: range1030613
version: 1
direction: direction/forward
top level: BBa_I13213
start: 1047
end: 1802
orientation: inline

BBa_R0062
type: SequenceAnnotation
persistent identity: annotation1030537
display id: annotation1030537
version: 1
top level: BBa_I13213
location: https://synbiohub.org/public/igem/BBa_I13213/annotation1030537/range1030537/1
component: BBa_R0062

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persistent identity: range1030537
display id: range1030537
version: 1
direction: direction/forward
top level: BBa_I13213
start: 1
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orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1030562
display id: annotation1030562
version: 1
top level: BBa_I13213
location: https://synbiohub.org/public/igem/BBa_I13213/annotation1030562/range1030562/1
component: BBa_B0010

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persistent identity: range1030562
display id: range1030562
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direction: direction/forward
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end: 812
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1030572
display id: annotation1030572
version: 1
top level: BBa_I13213
location: https://synbiohub.org/public/igem/BBa_I13213/annotation1030572/range1030572/1
component: BBa_B0012

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persistent identity: range1030572
display id: range1030572
version: 1
direction: direction/forward
top level: BBa_I13213
start: 821
end: 861
orientation: inline

BBa_C0061
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persistent identity: annotation1030555
display id: annotation1030555
version: 1
top level: BBa_I13213
location: https://synbiohub.org/public/igem/BBa_I13213/annotation1030555/range1030555/1
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persistent identity: range1030555
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direction: direction/forward
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orientation: inline

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type: SequenceAnnotation
persistent identity: annotation1030545
display id: annotation1030545
version: 1
top level: BBa_I13213
location: https://synbiohub.org/public/igem/BBa_I13213/annotation1030545/range1030545/1
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persistent identity: range1030545
display id: range1030545
version: 1
direction: direction/forward
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start: 64
end: 75
orientation: inline

BBa_R0063
type: SequenceAnnotation
persistent identity: annotation1030589
display id: annotation1030589
version: 1
top level: BBa_I13213
location: https://synbiohub.org/public/igem/BBa_I13213/annotation1030589/range1030589/1
component: BBa_R0063

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type: Range
persistent identity: range1030589
display id: range1030589
version: 1
direction: direction/forward
top level: BBa_I13213
start: 870
end: 1020
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1030598
display id: annotation1030598
version: 1
top level: BBa_I13213
location: https://synbiohub.org/public/igem/BBa_I13213/annotation1030598/range1030598/1
component: BBa_B0034

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type: Range
persistent identity: range1030598
display id: range1030598
version: 1
direction: direction/forward
top level: BBa_I13213
start: 1029
end: 1040
orientation: inline

BBa_K1072002
type: ComponentDefinition
persistent identity: BBa K1072002
display id: BBa_K1072002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: NADH dehydrogenase
creator: Yichong wang, Ruichen, Xihao liao and Junjie zhang
created: 2013-08-31T11:00:00Z
modified: 2015-05-08T01:09:01Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1381_
m user id: 0
owner id: 16002
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: BBa_K1072002 is the generator fo NADH dehydrogenase which is an enzyme following chemical reaction: NADH + H+ acceptor \rightleftharpoons NAD+ + reduced acceptor
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0034
component: BBa_B0012
component: BBa_B0010
component: BBa_K1072001
component: BBa_R0062
sequence annotation: BBa_B0012
sequence annotation: BBa_R0062
sequence annotation: BBa_B0034
sequence annotation: BBa_K1072001
sequence annotation: BBa_B0010
sequence: https://synbiohub.org/public/igem/BBa_K1072002_sequence/1

BBa_B0034
type: Component
persistent identity: component2334350
display id: component2334350
version: 1
top level: BBa_K1072002
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0012
type: Component
persistent identity: component2334355
display id: component2334355
version: 1
top level: BBa_K1072002
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0010
type: Component
persistent identity: component2334353
display id: component2334353
version: 1
top level: BBa_K1072002
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_K1072001
type: Component
persistent identity: component2334352
display id: component2334352
version: 1
top level: BBa_K1072002
access: public
definition: https://synbiohub.org/public/igem/BBa_K1072001/1

BBa_R0062
type: Component
persistent identity: component2334344
display id: component2334344
version: 1
top level: BBa_K1072002
access: public
definition: https://synbiohub.org/public/igem/BBa_R0062/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2334355
display id: annotation2334355
version: 1
top level: BBa_K1072002
location: https://synbiohub.org/public/igem/BBa_K1072002/annotation2334355/range2334355/1
component: BBa_B0012

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type: Range
persistent identity: range2334355
display id: range2334355
version: 1
direction: direction/forward
top level: BBa_K1072002
start: 1485
end: 1525
orientation: inline

BBa_R0062
type: SequenceAnnotation
persistent identity: annotation2334344
display id: annotation2334344
version: 1
top level: BBa_K1072002
location: https://synbiohub.org/public/igem/BBa_K1072002/annotation2334344/range2334344/1
component: BBa_R0062

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type: Range
persistent identity: range2334344
display id: range2334344
version: 1
direction: direction/forward
top level: BBa_K1072002
start: 1
end: 55
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2334350
display id: annotation2334350
version: 1
top level: BBa_K1072002
location: https://synbiohub.org/public/igem/BBa_K1072002/annotation2334350/range2334350/1
component: BBa_B0034

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type: Range
persistent identity: range2334350
display id: range2334350
version: 1
direction: direction/reverse
top level: BBa_K1072002
start: 64
end: 75
orientation: inline

BBa_K1072001
type: SequenceAnnotation
persistent identity: annotation2334352
display id: annotation2334352
version: 1
top level: BBa_K1072002
location: https://synbiohub.org/public/igem/BBa_K1072002/annotation2334352/range2334352/1
component: BBa_K1072001

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persistent identity: range2334352
display id: range2334352
version: 1
direction: direction/reverse
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start: 84
end: 1388
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2334353
display id: annotation2334353
version: 1
top level: BBa_K1072002
location: https://synbiohub.org/public/igem/BBa_K1072002/annotation2334353/range2334353/1
component: BBa_B0010

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type: Range
persistent identity: range2334353
display id: range2334353
version: 1
direction: direction/forward
top level: BBa_K1072002
start: 1397
end: 1476
orientation: inline

BBa_K1315007
type: ComponentDefinition
persistent identity: BBa K1315007
display id: BBa_K1315007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: The BCAM0228 Response Regulator from Burkholderia
creator: Dimitrios Michailidis
created: 2014-09-25T11:00:00Z
modified: 2015-05-08T01:09:50Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1690_
m user id: 0
owner id: 21812
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This is the coding sequence of the BCAM0228 two-component regulator of the BDSF sensory BCAM0227-BCAM0228 system. This system is found in Burkholderia spp.
mutable notes: The sequence had 1 EcoRI site that was mutated out by site-directed mutagenesis.
mutable provenance: The source of this part is Burkholderia cenocepacia J2315 genomic DNA which was provided by the Robert Ryan lab in the Molecular Microbiology department of the University of Dundee, UK.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: BCAM0228
sequence: https://synbiohub.org/public/igem/BBa_K1315007_sequence/1

BCAM0228
type: SequenceAnnotation
persistent identity: annotation2386928
display id: annotation2386928
version: 1
top level: BBa_K1315007
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1315007/annotation2386928/range2386928/1

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type: Range
persistent identity: range2386928
display id: range2386928
version: 1
direction: direction/forward
top level: BBa_K1315007
start: 1
end: 888
orientation: inline

BBa_I0428
type: ComponentDefinition
persistent identity: BBa I0428
display id: BBa_I0428
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: I0408.I6106
creator: jcbraff
created: 2004-07-13T11:00:00Z
modified: 2015-08-31T04:07:29Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_1_
m user id: 0
owner id: 61
owning group id: 7
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: I0408.I6106 Cin test
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Signalling
role: SO:0000804
component: BBa_R0040
component: BBa_B0034
component: BBa_B0010
component: BBa_E0020
component: BBa_B0012
component: BBa_C0077
component: BBa_R0078
component: BBa_E0030
sequence annotation: BBa_B0034
sequence annotation: BBa_E0020
sequence annotation: BBa_E0030
sequence annotation: BBa_R0040
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_R0078
sequence annotation: BBa_C0077
sequence: https://synbiohub.org/public/igem/BBa_I0428_sequence/1

BBa_R0040
type: Component
persistent identity: component973904
display id: component973904
version: 1
top level: BBa_I0428
access: public
definition: https://synbiohub.org/public/igem/BBa_R0040/1

BBa_B0034
type: Component
persistent identity: component973964
display id: component973964
version: 1
top level: BBa_I0428
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0010
type: Component
persistent identity: component973971
display id: component973971
version: 1
top level: BBa_I0428
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0010
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persistent identity: component973935
display id: component973935
version: 1
top level: BBa_I0428
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_E0020
type: Component
persistent identity: component973930
display id: component973930
version: 1
top level: BBa_I0428
access: public
definition: https://synbiohub.org/public/igem/BBa_E0020/1

BBa_B0034
type: Component
persistent identity: component973928
display id: component973928
version: 1
top level: BBa_I0428
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0012
type: Component
persistent identity: component973945
display id: component973945
version: 1
top level: BBa_I0428
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_C0077
type: Component
persistent identity: component973922
display id: component973922
version: 1
top level: BBa_I0428
access: public
definition: cinr

BBa_B0012
type: Component
persistent identity: component973981
display id: component973981
version: 1
top level: BBa_I0428
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_R0078
type: Component
persistent identity: component973959
display id: component973959
version: 1
top level: BBa_I0428
access: public
definition: https://synbiohub.org/public/igem/BBa_R0078/1

BBa_B0034
type: Component
persistent identity: component973912
display id: component973912
version: 1
top level: BBa_I0428
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_E0030
type: Component
persistent identity: component973966
display id: component973966
version: 1
top level: BBa_I0428
access: public
definition: https://synbiohub.org/public/igem/BBa_E0030/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation973928
display id: annotation973928
version: 1
top level: BBa_I0428
location: https://synbiohub.org/public/igem/BBa_I0428/annotation973928/range973928/1
component: BBa_B0034

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type: Range
persistent identity: range973928
display id: range973928
version: 1
direction: direction/forward
top level: BBa_I0428
start: 876
end: 887
orientation: inline

BBa_E0020
type: SequenceAnnotation
persistent identity: annotation973930
display id: annotation973930
version: 1
top level: BBa_I0428
location: https://synbiohub.org/public/igem/BBa_I0428/annotation973930/range973930/1
component: BBa_E0020

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type: Range
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display id: range973930
version: 1
direction: direction/forward
top level: BBa_I0428
start: 894
end: 1616
orientation: inline

BBa_E0030
type: SequenceAnnotation
persistent identity: annotation973966
display id: annotation973966
version: 1
top level: BBa_I0428
location: https://synbiohub.org/public/igem/BBa_I0428/annotation973966/range973966/1
component: BBa_E0030

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type: Range
persistent identity: range973966
display id: range973966
version: 1
direction: direction/forward
top level: BBa_I0428
start: 2013
end: 2735
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation973904
display id: annotation973904
version: 1
top level: BBa_I0428
location: https://synbiohub.org/public/igem/BBa_I0428/annotation973904/range973904/1
component: BBa_R0040

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type: Range
persistent identity: range973904
display id: range973904
version: 1
direction: direction/forward
top level: BBa_I0428
start: 1
end: 54
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation973935
display id: annotation973935
version: 1
top level: BBa_I0428
location: https://synbiohub.org/public/igem/BBa_I0428/annotation973935/range973935/1
component: BBa_B0010

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type: Range
persistent identity: range973935
display id: range973935
version: 1
direction: direction/forward
top level: BBa_I0428
start: 1625
end: 1704
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation973964
display id: annotation973964
version: 1
top level: BBa_I0428
location: https://synbiohub.org/public/igem/BBa_I0428/annotation973964/range973964/1
component: BBa_B0034

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type: Range
persistent identity: range973964
display id: range973964
version: 1
direction: direction/forward
top level: BBa_I0428
start: 1995
end: 2006
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation973945
display id: annotation973945
version: 1
top level: BBa_I0428
location: https://synbiohub.org/public/igem/BBa_I0428/annotation973945/range973945/1
component: BBa_B0012

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type: Range
persistent identity: range973945
display id: range973945
version: 1
direction: direction/forward
top level: BBa_I0428
start: 1713
end: 1753
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation973981
display id: annotation973981
version: 1
top level: BBa_I0428
location: https://synbiohub.org/public/igem/BBa_I0428/annotation973981/range973981/1
component: BBa_B0012

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type: Range
persistent identity: range973981
display id: range973981
version: 1
direction: direction/forward
top level: BBa_I0428
start: 2832
end: 2872
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation973971
display id: annotation973971
version: 1
top level: BBa_I0428
location: https://synbiohub.org/public/igem/BBa_I0428/annotation973971/range973971/1
component: BBa_B0010

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type: Range
persistent identity: range973971
display id: range973971
version: 1
direction: direction/forward
top level: BBa_I0428
start: 2744
end: 2823
orientation: inline

BBa_R0078
type: SequenceAnnotation
persistent identity: annotation973959
display id: annotation973959
version: 1
top level: BBa_I0428
location: https://synbiohub.org/public/igem/BBa_I0428/annotation973959/range973959/1
component: BBa_R0078

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type: Range
persistent identity: range973959
display id: range973959
version: 1
direction: direction/forward
top level: BBa_I0428
start: 1762
end: 1986
orientation: inline

BBa_C0077
type: SequenceAnnotation
persistent identity: annotation973922
display id: annotation973922
version: 1
top level: BBa_I0428
location: https://synbiohub.org/public/igem/BBa_I0428/annotation973922/range973922/1
component: BBa_C0077

https://synbiohub.org/public/igem/BBa_I0428/annotation973922/range973922/1
type: Range
persistent identity: range973922
display id: range973922
version: 1
direction: direction/forward
top level: BBa_I0428
start: 81
end: 842
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation973912
display id: annotation973912
version: 1
top level: BBa_I0428
location: https://synbiohub.org/public/igem/BBa_I0428/annotation973912/range973912/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I0428/annotation973912/range973912/1
type: Range
persistent identity: range973912
display id: range973912
version: 1
direction: direction/forward
top level: BBa_I0428
start: 63
end: 74
orientation: inline

BBa_J06002
type: ComponentDefinition
persistent identity: BBa J06002
display id: BBa_J06002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Tet repressible luxI generator ( LVA- )
creator: sash31
created: 2005-06-12T11:00:00Z
modified: 2015-08-31T04:08:16Z
discontinued: false
dominant: true
experience: experience/None
group u list: _20_
m user id: 0
owner id: 339
owning group id: 20
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: A part designed to emit AHL when given aTc. Uses R0040 and F1611.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Signalling
role: SO:0000804
component: BBa_B0034
component: BBa_C0161
component: BBa_R0040
component: BBa_B0010
component: BBa_B0012
sequence annotation: BBa_B0010
sequence annotation: BBa_R0040
sequence annotation: BBa_B0012
sequence annotation: BBa_C0161
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_J06002_sequence/1

BBa_B0034
type: Component
persistent identity: component1512999
display id: component1512999
version: 1
top level: BBa_J06002
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_C0161
type: Component
persistent identity: component1513001
display id: component1513001
version: 1
top level: BBa_J06002
access: public
definition: luxI

BBa_R0040
type: Component
persistent identity: component1512991
display id: component1512991
version: 1
top level: BBa_J06002
access: public
definition: https://synbiohub.org/public/igem/BBa_R0040/1

BBa_B0010
type: Component
persistent identity: component1513006
display id: component1513006
version: 1
top level: BBa_J06002
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
type: Component
persistent identity: component1513016
display id: component1513016
version: 1
top level: BBa_J06002
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1513006
display id: annotation1513006
version: 1
top level: BBa_J06002
location: https://synbiohub.org/public/igem/BBa_J06002/annotation1513006/range1513006/1
component: BBa_B0010

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type: Range
persistent identity: range1513006
display id: range1513006
version: 1
direction: direction/forward
top level: BBa_J06002
start: 674
end: 753
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1512991
display id: annotation1512991
version: 1
top level: BBa_J06002
location: https://synbiohub.org/public/igem/BBa_J06002/annotation1512991/range1512991/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_J06002/annotation1512991/range1512991/1
type: Range
persistent identity: range1512991
display id: range1512991
version: 1
direction: direction/forward
top level: BBa_J06002
start: 1
end: 54
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1513016
display id: annotation1513016
version: 1
top level: BBa_J06002
location: https://synbiohub.org/public/igem/BBa_J06002/annotation1513016/range1513016/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_J06002/annotation1513016/range1513016/1
type: Range
persistent identity: range1513016
display id: range1513016
version: 1
direction: direction/forward
top level: BBa_J06002
start: 762
end: 802
orientation: inline

BBa_C0161
type: SequenceAnnotation
persistent identity: annotation1513001
display id: annotation1513001
version: 1
top level: BBa_J06002
location: https://synbiohub.org/public/igem/BBa_J06002/annotation1513001/range1513001/1
component: BBa_C0161

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type: Range
persistent identity: range1513001
display id: range1513001
version: 1
direction: direction/forward
top level: BBa_J06002
start: 81
end: 665
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1512999
display id: annotation1512999
version: 1
top level: BBa_J06002
location: https://synbiohub.org/public/igem/BBa_J06002/annotation1512999/range1512999/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_J06002/annotation1512999/range1512999/1
type: Range
persistent identity: range1512999
display id: range1512999
version: 1
direction: direction/forward
top level: BBa_J06002
start: 63
end: 74
orientation: inline

BBa_K1631022
type: ComponentDefinition
persistent identity: BBa K1631022
display id: BBa_K1631022
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Plux -rbs- Barnase -rbs- GFP -d.term
creator: Yuto Yamanaka
created: 2015-09-13T11:00:00Z
modified: 2015-09-18T06:26:39Z
discontinued: false
dominant: false
group u list: _2048_
m user id: 23567
owner id: 23567
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Barnase and GFP is co-expressed by luxR/AHL complex.
mutable notes: none
mutable provenance: Assembly
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K1631001
component: BBa_R0062
sequence annotation: BBa_K1631001
sequence annotation: BBa_R0062
sequence: https://synbiohub.org/public/igem/BBa_K1631022_sequence/1

BBa_K1631001
type: Component
persistent identity: component2455743
display id: component2455743
version: 1
top level: BBa_K1631022
access: public
definition: https://synbiohub.org/public/igem/BBa_K1631001/1

BBa_R0062
type: Component
persistent identity: component2455723
display id: component2455723
version: 1
top level: BBa_K1631022
access: public
definition: https://synbiohub.org/public/igem/BBa_R0062/1

BBa_K1631001
type: SequenceAnnotation
persistent identity: annotation2455743
display id: annotation2455743
version: 1
top level: BBa_K1631022
location: https://synbiohub.org/public/igem/BBa_K1631022/annotation2455743/range2455743/1
component: BBa_K1631001

https://synbiohub.org/public/igem/BBa_K1631022/annotation2455743/range2455743/1
type: Range
persistent identity: range2455743
display id: range2455743
version: 1
direction: direction/reverse
top level: BBa_K1631022
start: 64
end: 1330
orientation: inline

BBa_R0062
type: SequenceAnnotation
persistent identity: annotation2455723
display id: annotation2455723
version: 1
top level: BBa_K1631022
location: https://synbiohub.org/public/igem/BBa_K1631022/annotation2455723/range2455723/1
component: BBa_R0062

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type: Range
persistent identity: range2455723
display id: range2455723
version: 1
direction: direction/forward
top level: BBa_K1631022
start: 1
end: 55
orientation: inline

BBa_I0426
type: ComponentDefinition
persistent identity: BBa I0426
display id: BBa_I0426
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: I0406.I6107
creator: jcbraff
created: 2004-07-13T11:00:00Z
modified: 2015-08-31T04:07:29Z
discontinued: false
dominant: false
experience: experience/None
group u list: _101_
m user id: 0
owner id: 61
owning group id: 7
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: I0406.I6107 Las test
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Signalling
role: SO:0000804
component: BBa_B0012
component: BBa_R0040
component: BBa_E0030
component: BBa_B0010
component: BBa_B0034
component: BBa_E0020
component: BBa_C0079
component: BBa_R0079
sequence annotation: BBa_R0040
sequence annotation: BBa_B0012
sequence annotation: BBa_B0034
sequence annotation: BBa_R0079
sequence annotation: BBa_B0010
sequence annotation: BBa_E0030
sequence annotation: BBa_C0079
sequence annotation: BBa_E0020
sequence: https://synbiohub.org/public/igem/BBa_I0426_sequence/1

BBa_B0012
type: Component
persistent identity: component973894
display id: component973894
version: 1
top level: BBa_I0426
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_R0040
type: Component
persistent identity: component973811
display id: component973811
version: 1
top level: BBa_I0426
access: public
definition: https://synbiohub.org/public/igem/BBa_R0040/1

BBa_E0030
type: Component
persistent identity: component973879
display id: component973879
version: 1
top level: BBa_I0426
access: public
definition: https://synbiohub.org/public/igem/BBa_E0030/1

BBa_B0010
type: Component
persistent identity: component973845
display id: component973845
version: 1
top level: BBa_I0426
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0034
type: Component
persistent identity: component973838
display id: component973838
version: 1
top level: BBa_I0426
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_E0020
type: Component
persistent identity: component973840
display id: component973840
version: 1
top level: BBa_I0426
access: public
definition: https://synbiohub.org/public/igem/BBa_E0020/1

BBa_B0034
type: Component
persistent identity: component973877
display id: component973877
version: 1
top level: BBa_I0426
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_C0079
type: Component
persistent identity: component973832
display id: component973832
version: 1
top level: BBa_I0426
access: public
definition: lasr

BBa_B0012
type: Component
persistent identity: component973855
display id: component973855
version: 1
top level: BBa_I0426
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0010
type: Component
persistent identity: component973884
display id: component973884
version: 1
top level: BBa_I0426
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_R0079
type: Component
persistent identity: component973872
display id: component973872
version: 1
top level: BBa_I0426
access: public
definition: https://synbiohub.org/public/igem/BBa_R0079/1

BBa_B0034
type: Component
persistent identity: component973819
display id: component973819
version: 1
top level: BBa_I0426
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation973811
display id: annotation973811
version: 1
top level: BBa_I0426
location: https://synbiohub.org/public/igem/BBa_I0426/annotation973811/range973811/1
component: BBa_R0040

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type: Range
persistent identity: range973811
display id: range973811
version: 1
direction: direction/forward
top level: BBa_I0426
start: 1
end: 54
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation973894
display id: annotation973894
version: 1
top level: BBa_I0426
location: https://synbiohub.org/public/igem/BBa_I0426/annotation973894/range973894/1
component: BBa_B0012

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type: Range
persistent identity: range973894
display id: range973894
version: 1
direction: direction/forward
top level: BBa_I0426
start: 2758
end: 2798
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation973819
display id: annotation973819
version: 1
top level: BBa_I0426
location: https://synbiohub.org/public/igem/BBa_I0426/annotation973819/range973819/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I0426/annotation973819/range973819/1
type: Range
persistent identity: range973819
display id: range973819
version: 1
direction: direction/forward
top level: BBa_I0426
start: 63
end: 74
orientation: inline

BBa_R0079
type: SequenceAnnotation
persistent identity: annotation973872
display id: annotation973872
version: 1
top level: BBa_I0426
location: https://synbiohub.org/public/igem/BBa_I0426/annotation973872/range973872/1
component: BBa_R0079

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type: Range
persistent identity: range973872
display id: range973872
version: 1
direction: direction/forward
top level: BBa_I0426
start: 1756
end: 1912
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation973884
display id: annotation973884
version: 1
top level: BBa_I0426
location: https://synbiohub.org/public/igem/BBa_I0426/annotation973884/range973884/1
component: BBa_B0010

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type: Range
persistent identity: range973884
display id: range973884
version: 1
direction: direction/forward
top level: BBa_I0426
start: 2670
end: 2749
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation973845
display id: annotation973845
version: 1
top level: BBa_I0426
location: https://synbiohub.org/public/igem/BBa_I0426/annotation973845/range973845/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I0426/annotation973845/range973845/1
type: Range
persistent identity: range973845
display id: range973845
version: 1
direction: direction/forward
top level: BBa_I0426
start: 1619
end: 1698
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation973838
display id: annotation973838
version: 1
top level: BBa_I0426
location: https://synbiohub.org/public/igem/BBa_I0426/annotation973838/range973838/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I0426/annotation973838/range973838/1
type: Range
persistent identity: range973838
display id: range973838
version: 1
direction: direction/forward
top level: BBa_I0426
start: 870
end: 881
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation973855
display id: annotation973855
version: 1
top level: BBa_I0426
location: https://synbiohub.org/public/igem/BBa_I0426/annotation973855/range973855/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I0426/annotation973855/range973855/1
type: Range
persistent identity: range973855
display id: range973855
version: 1
direction: direction/forward
top level: BBa_I0426
start: 1707
end: 1747
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation973877
display id: annotation973877
version: 1
top level: BBa_I0426
location: https://synbiohub.org/public/igem/BBa_I0426/annotation973877/range973877/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I0426/annotation973877/range973877/1
type: Range
persistent identity: range973877
display id: range973877
version: 1
direction: direction/forward
top level: BBa_I0426
start: 1921
end: 1932
orientation: inline

BBa_E0030
type: SequenceAnnotation
persistent identity: annotation973879
display id: annotation973879
version: 1
top level: BBa_I0426
location: https://synbiohub.org/public/igem/BBa_I0426/annotation973879/range973879/1
component: BBa_E0030

https://synbiohub.org/public/igem/BBa_I0426/annotation973879/range973879/1
type: Range
persistent identity: range973879
display id: range973879
version: 1
direction: direction/forward
top level: BBa_I0426
start: 1939
end: 2661
orientation: inline

BBa_C0079
type: SequenceAnnotation
persistent identity: annotation973832
display id: annotation973832
version: 1
top level: BBa_I0426
location: https://synbiohub.org/public/igem/BBa_I0426/annotation973832/range973832/1
component: BBa_C0079

https://synbiohub.org/public/igem/BBa_I0426/annotation973832/range973832/1
type: Range
persistent identity: range973832
display id: range973832
version: 1
direction: direction/forward
top level: BBa_I0426
start: 81
end: 836
orientation: inline

BBa_E0020
type: SequenceAnnotation
persistent identity: annotation973840
display id: annotation973840
version: 1
top level: BBa_I0426
location: https://synbiohub.org/public/igem/BBa_I0426/annotation973840/range973840/1
component: BBa_E0020

https://synbiohub.org/public/igem/BBa_I0426/annotation973840/range973840/1
type: Range
persistent identity: range973840
display id: range973840
version: 1
direction: direction/forward
top level: BBa_I0426
start: 888
end: 1610
orientation: inline

LuxO D47E
type: ComponentDefinition
persistent identity: BBa K131022
display id: BBa_K131022
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LuxO D47E, Vibrio harveyi
creator: Thane Kubik
created: 2008-10-27T12:00:00Z
modified: 2015-05-08T01:09:47Z
discontinued: false
dominant: true
experience: experience/None
group u list: _259_
m user id: 0
owner id: 3190
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: LuxO D47E, Vibrio harveyi is a constitutively active mutant of LuxO
mutable notes: The plasmid containing LuxOD47E (pJAF822) was obtained from Bassler lab (Freeman and Bassler, 1999). Jeremy A. Freeman and Bonnie L. Bassler. A genetic analysis of the function of LuxO, a two-component response regulator involved in quorum sensing in Vibrio harveyi. 1999a. Molecular Microbiology. 31(2), 665-677.
mutable provenance: >gi|156972381:c2983472-2982069 Vibrio harveyi ATCC BAA-1116 chromosome I, complete sequence Nucleotide number 141 has been mutated from T to G, thus bases 139-141 have been changed from GAT (Aspartic acid, D) to GAG (Glutamine, E). Jeremy A. Freeman and Bonnie L. Bassler. A genetic analysis of the function of LuxO, a two-component response regulator involved in quorum sensing in Vibrio harveyi. 1999a. Molecular Microbiology. 31(2), 665-677.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Stop
sequence annotation: D47E
sequence annotation: Start
sequence: https://synbiohub.org/public/igem/BBa_K131022_sequence/1

Stop
type: SequenceAnnotation
persistent identity: annotation1990656
display id: annotation1990656
version: 1
top level: LuxO D47E
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K131022/annotation1990656/range1990656/1

https://synbiohub.org/public/igem/BBa_K131022/annotation1990656/range1990656/1
type: Range
persistent identity: range1990656
display id: range1990656
version: 1
direction: direction/forward
top level: LuxO D47E
start: 1360
end: 1362
orientation: inline

D47E
type: SequenceAnnotation
persistent identity: annotation1990654
display id: annotation1990654
version: 1
top level: LuxO D47E
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K131022/annotation1990654/range1990654/1

https://synbiohub.org/public/igem/BBa_K131022/annotation1990654/range1990654/1
type: Range
persistent identity: range1990654
display id: range1990654
version: 1
direction: direction/forward
top level: LuxO D47E
start: 139
end: 141
orientation: inline

Start
type: SequenceAnnotation
persistent identity: annotation1990655
display id: annotation1990655
version: 1
top level: LuxO D47E
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K131022/annotation1990655/range1990655/1

https://synbiohub.org/public/igem/BBa_K131022/annotation1990655/range1990655/1
type: Range
persistent identity: range1990655
display id: range1990655
version: 1
direction: direction/forward
top level: LuxO D47E
start: 1
end: 3
orientation: inline

BBa_K990001
type: ComponentDefinition
persistent identity: BBa K990001
display id: BBa_K990001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pOmpC+EPIC Firefly Luciferase
creator: Fatma Bet??l ??EVİK
created: 2013-06-20T11:00:00Z
modified: 2015-05-08T01:13:50Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1287_
m user id: 0
owner id: 11713
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This part is the one of the responding systems of the receiver device of our Photocommunication system
mutable notes: We used ncbi when we couldn't find sequence
mutable provenance: EPIC Luciferase: BBa_K325100, Cambridge 2010 pOmpC: BBa_R0082
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Signalling
role: SO:0000804
component: BBa_K325100
component: BBa_R0082
sequence annotation: BBa_K325100
sequence annotation: BBa_R0082
sequence: https://synbiohub.org/public/igem/BBa_K990001_sequence/1

BBa_K325100
type: Component
persistent identity: component2329858
display id: component2329858
version: 1
top level: BBa_K990001
access: public
definition: https://synbiohub.org/public/igem/BBa_K325100/1

BBa_R0082
type: Component
persistent identity: component2329850
display id: component2329850
version: 1
top level: BBa_K990001
access: public
definition: https://synbiohub.org/public/igem/BBa_R0082/1

BBa_K325100
type: SequenceAnnotation
persistent identity: annotation2329858
display id: annotation2329858
version: 1
top level: BBa_K990001
location: https://synbiohub.org/public/igem/BBa_K990001/annotation2329858/range2329858/1
component: BBa_K325100

https://synbiohub.org/public/igem/BBa_K990001/annotation2329858/range2329858/1
type: Range
persistent identity: range2329858
display id: range2329858
version: 1
direction: direction/reverse
top level: BBa_K990001
start: 117
end: 2742
orientation: inline

BBa_R0082
type: SequenceAnnotation
persistent identity: annotation2329850
display id: annotation2329850
version: 1
top level: BBa_K990001
location: https://synbiohub.org/public/igem/BBa_K990001/annotation2329850/range2329850/1
component: BBa_R0082

https://synbiohub.org/public/igem/BBa_K990001/annotation2329850/range2329850/1
type: Range
persistent identity: range2329850
display id: range2329850
version: 1
direction: direction/reverse
top level: BBa_K990001
start: 1
end: 108
orientation: inline

Pgad
type: ComponentDefinition
persistent identity: BBa K559011
display id: BBa_K559011
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Pgad - Intracellular Chloride-Sensing Cassette
creator: Jacky, Fong Chuen, Loo
created: 2011-10-27T11:00:00Z
modified: 2015-05-08T01:12:41Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _727_
m user id: 0
owner id: 6123
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: Pgad is chloride-sensitive promoter which was first discovered in Lactococcus lactis1, which is a gram-positive bacterium which can live in acidic environment. In this operon, gadC is glutamate-gamma-aminobutyrate antiporter and gadB is glutamate decarboxylase. They are both involved in intracellular pH regulation and co-expressed in the same operon under the control of Pgad2. The gene before Pgad, named gadR, which is constitutively expressed under the control of PgadR, is a positive regulator of Pgad coupled genes while intracellular chloride is level elevated.
mutable notes: Synthesized part by GenScript,all the illegal sites for RFC 10 (EcoRI, XbaI, SpeI, PstI) have been substituted by other codon producing the same amino acid in E. coli.
mutable provenance: The gene sequence was obtained from the cassette from bp 821 to 2071 of GenBank sequence AF005098, which includes PgadR, gadR, Pgad and the starting codon ATG.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: Pgad
sequence annotation: RBS_gadC
sequence annotation: PgadR
sequence annotation: gadC Start
sequence annotation: RBS_gadR
sequence annotation: gadR
sequence: https://synbiohub.org/public/igem/BBa_K559011_sequence/1

Pgad
type: SequenceAnnotation
persistent identity: annotation2160939
display id: annotation2160939
version: 1
top level: Pgad
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K559011/annotation2160939/range2160939/1

https://synbiohub.org/public/igem/BBa_K559011/annotation2160939/range2160939/1
type: Range
persistent identity: range2160939
display id: range2160939
version: 1
direction: direction/forward
top level: Pgad
start: 1163
end: 1181
orientation: inline

RBS_gadC
type: SequenceAnnotation
persistent identity: annotation2160940
display id: annotation2160940
version: 1
top level: Pgad
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K559011/annotation2160940/range2160940/1

https://synbiohub.org/public/igem/BBa_K559011/annotation2160940/range2160940/1
type: Range
persistent identity: range2160940
display id: range2160940
version: 1
direction: direction/forward
top level: Pgad
start: 1237
end: 1245
orientation: inline

PgadR
type: SequenceAnnotation
persistent identity: annotation2160937
display id: annotation2160937
version: 1
top level: Pgad
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K559011/annotation2160937/range2160937/1

https://synbiohub.org/public/igem/BBa_K559011/annotation2160937/range2160937/1
type: Range
persistent identity: range2160937
display id: range2160937
version: 1
direction: direction/forward
top level: Pgad
start: 141
end: 158
orientation: inline

gadC Start
type: SequenceAnnotation
persistent identity: annotation2160936
display id: annotation2160936
version: 1
top level: Pgad
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K559011/annotation2160936/range2160936/1

https://synbiohub.org/public/igem/BBa_K559011/annotation2160936/range2160936/1
type: Range
persistent identity: range2160936
display id: range2160936
version: 1
direction: direction/forward
top level: Pgad
start: 1249
end: 1251
orientation: inline

RBS_gadR
type: SequenceAnnotation
persistent identity: annotation2160941
display id: annotation2160941
version: 1
top level: Pgad
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K559011/annotation2160941/range2160941/1

https://synbiohub.org/public/igem/BBa_K559011/annotation2160941/range2160941/1
type: Range
persistent identity: range2160941
display id: range2160941
version: 1
direction: direction/forward
top level: Pgad
start: 264
end: 269
orientation: inline

gadR
type: SequenceAnnotation
persistent identity: annotation2160938
display id: annotation2160938
version: 1
top level: Pgad
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K559011/annotation2160938/range2160938/1

https://synbiohub.org/public/igem/BBa_K559011/annotation2160938/range2160938/1
type: Range
persistent identity: range2160938
display id: range2160938
version: 1
direction: direction/forward
top level: Pgad
start: 275
end: 1105
orientation: inline

BBa_K082008
type: ComponentDefinition
persistent identity: BBa K082008
display id: BBa_K082008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: B0032-C0070
creator: Zhen Xu , Binjie Xu
created: 2008-10-27T12:00:00Z
modified: 2015-05-08T01:08:35Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _228_
m user id: 0
owner id: 1560
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: none
mutable notes: none
mutable provenance: none
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_B0032
component: BBa_C0070
sequence annotation: BBa_C0070
sequence annotation: BBa_B0032
sequence: https://synbiohub.org/public/igem/BBa_K082008_sequence/1

BBa_B0032
type: Component
persistent identity: component2244046
display id: component2244046
version: 1
top level: BBa_K082008
access: public
definition: https://synbiohub.org/public/igem/BBa_B0032/1

BBa_C0070
type: Component
persistent identity: component2244051
display id: component2244051
version: 1
top level: BBa_K082008
access: public
definition: rhII

BBa_C0070
type: SequenceAnnotation
persistent identity: annotation2244051
display id: annotation2244051
version: 1
top level: BBa_K082008
location: https://synbiohub.org/public/igem/BBa_K082008/annotation2244051/range2244051/1
component: BBa_C0070

https://synbiohub.org/public/igem/BBa_K082008/annotation2244051/range2244051/1
type: Range
persistent identity: range2244051
display id: range2244051
version: 1
direction: direction/reverse
top level: BBa_K082008
start: 20
end: 686
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation2244046
display id: annotation2244046
version: 1
top level: BBa_K082008
location: https://synbiohub.org/public/igem/BBa_K082008/annotation2244046/range2244046/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_K082008/annotation2244046/range2244046/1
type: Range
persistent identity: range2244046
display id: range2244046
version: 1
direction: direction/forward
top level: BBa_K082008
start: 1
end: 13
orientation: inline

BBa_I13205
type: ComponentDefinition
persistent identity: BBa I13205
display id: BBa_I13205
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: HSL/aiiA test construct
creator: Barry Canton
created: 2004-07-29T11:00:00Z
modified: 2015-08-31T04:07:33Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_6_
m user id: 0
owner id: 135
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: LuxR receiver circuit with an inducible promoter controlling aiiA production downstream. Useful to measure the ability of aiiA (LVA+) to turn off a pLuxL driven receiver circuit in the presence of exogenous HSL
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Signalling
role: SO:0000804
component: BBa_B0034
component: BBa_C0062
component: BBa_B0012
component: BBa_E0030
component: BBa_B0010
component: BBa_I0500
component: BBa_R0063
component: BBa_R0062
component: BBa_C0060
sequence annotation: BBa_C0060
sequence annotation: BBa_R0063
sequence annotation: BBa_B0034
sequence annotation: BBa_B0012
sequence annotation: BBa_B0010
sequence annotation: BBa_C0062
sequence annotation: BBa_I0500
sequence annotation: BBa_R0062
sequence annotation: BBa_E0030
sequence: https://synbiohub.org/public/igem/BBa_I13205_sequence/1

BBa_B0034
type: Component
persistent identity: component1043940
display id: component1043940
version: 1
top level: BBa_I13205
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_C0062
type: Component
persistent identity: component1043899
display id: component1043899
version: 1
top level: BBa_I13205
access: public
definition: luxr

BBa_B0012
type: Component
persistent identity: component1043957
display id: component1043957
version: 1
top level: BBa_I13205
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_E0030
type: Component
persistent identity: component1043942
display id: component1043942
version: 1
top level: BBa_I13205
access: public
definition: https://synbiohub.org/public/igem/BBa_E0030/1

BBa_B0010
type: Component
persistent identity: component1043990
display id: component1043990
version: 1
top level: BBa_I13205
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_I0500
type: Component
persistent identity: component1043962
display id: component1043962
version: 1
top level: BBa_I13205
access: public
definition: https://synbiohub.org/public/igem/BBa_I0500/1

BBa_B0010
type: Component
persistent identity: component1043907
display id: component1043907
version: 1
top level: BBa_I13205
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
type: Component
persistent identity: component1043917
display id: component1043917
version: 1
top level: BBa_I13205
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_R0063
type: Component
persistent identity: component1043875
display id: component1043875
version: 1
top level: BBa_I13205
access: public
definition: https://synbiohub.org/public/igem/BBa_R0063/1

BBa_B0034
type: Component
persistent identity: component1043884
display id: component1043884
version: 1
top level: BBa_I13205
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0010
type: Component
persistent identity: component1043947
display id: component1043947
version: 1
top level: BBa_I13205
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0034
type: Component
persistent identity: component1043967
display id: component1043967
version: 1
top level: BBa_I13205
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_R0062
type: Component
persistent identity: component1043932
display id: component1043932
version: 1
top level: BBa_I13205
access: public
definition: https://synbiohub.org/public/igem/BBa_R0062/1

BBa_B0012
type: Component
persistent identity: component1044000
display id: component1044000
version: 1
top level: BBa_I13205
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_C0060
type: Component
persistent identity: component1043982
display id: component1043982
version: 1
top level: BBa_I13205
access: public
definition: aiiA

BBa_C0060
type: SequenceAnnotation
persistent identity: annotation1043982
display id: annotation1043982
version: 1
top level: BBa_I13205
location: https://synbiohub.org/public/igem/BBa_I13205/annotation1043982/range1043982/1
component: BBa_C0060

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type: Range
persistent identity: range1043982
display id: range1043982
version: 1
direction: direction/forward
top level: BBa_I13205
start: 3289
end: 4077
orientation: inline

BBa_R0063
type: SequenceAnnotation
persistent identity: annotation1043875
display id: annotation1043875
version: 1
top level: BBa_I13205
location: https://synbiohub.org/public/igem/BBa_I13205/annotation1043875/range1043875/1
component: BBa_R0063

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type: Range
persistent identity: range1043875
display id: range1043875
version: 1
direction: direction/forward
top level: BBa_I13205
start: 1
end: 151
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1043940
display id: annotation1043940
version: 1
top level: BBa_I13205
location: https://synbiohub.org/public/igem/BBa_I13205/annotation1043940/range1043940/1
component: BBa_B0034

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type: Range
persistent identity: range1043940
display id: range1043940
version: 1
direction: direction/forward
top level: BBa_I13205
start: 1167
end: 1178
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1043957
display id: annotation1043957
version: 1
top level: BBa_I13205
location: https://synbiohub.org/public/igem/BBa_I13205/annotation1043957/range1043957/1
component: BBa_B0012

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type: Range
persistent identity: range1043957
display id: range1043957
version: 1
direction: direction/forward
top level: BBa_I13205
start: 2004
end: 2044
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1043990
display id: annotation1043990
version: 1
top level: BBa_I13205
location: https://synbiohub.org/public/igem/BBa_I13205/annotation1043990/range1043990/1
component: BBa_B0010

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type: Range
persistent identity: range1043990
display id: range1043990
version: 1
direction: direction/forward
top level: BBa_I13205
start: 4111
end: 4190
orientation: inline

BBa_C0062
type: SequenceAnnotation
persistent identity: annotation1043899
display id: annotation1043899
version: 1
top level: BBa_I13205
location: https://synbiohub.org/public/igem/BBa_I13205/annotation1043899/range1043899/1
component: BBa_C0062

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type: Range
persistent identity: range1043899
display id: range1043899
version: 1
direction: direction/forward
top level: BBa_I13205
start: 178
end: 933
orientation: inline

BBa_I0500
type: SequenceAnnotation
persistent identity: annotation1043962
display id: annotation1043962
version: 1
top level: BBa_I13205
location: https://synbiohub.org/public/igem/BBa_I13205/annotation1043962/range1043962/1
component: BBa_I0500

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type: Range
persistent identity: range1043962
display id: range1043962
version: 1
direction: direction/forward
top level: BBa_I13205
start: 2053
end: 3262
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1043917
display id: annotation1043917
version: 1
top level: BBa_I13205
location: https://synbiohub.org/public/igem/BBa_I13205/annotation1043917/range1043917/1
component: BBa_B0012

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type: Range
persistent identity: range1043917
display id: range1043917
version: 1
direction: direction/forward
top level: BBa_I13205
start: 1055
end: 1095
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1043967
display id: annotation1043967
version: 1
top level: BBa_I13205
location: https://synbiohub.org/public/igem/BBa_I13205/annotation1043967/range1043967/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I13205/annotation1043967/range1043967/1
type: Range
persistent identity: range1043967
display id: range1043967
version: 1
direction: direction/forward
top level: BBa_I13205
start: 3271
end: 3282
orientation: inline

BBa_R0062
type: SequenceAnnotation
persistent identity: annotation1043932
display id: annotation1043932
version: 1
top level: BBa_I13205
location: https://synbiohub.org/public/igem/BBa_I13205/annotation1043932/range1043932/1
component: BBa_R0062

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type: Range
persistent identity: range1043932
display id: range1043932
version: 1
direction: direction/forward
top level: BBa_I13205
start: 1104
end: 1158
orientation: inline

BBa_E0030
type: SequenceAnnotation
persistent identity: annotation1043942
display id: annotation1043942
version: 1
top level: BBa_I13205
location: https://synbiohub.org/public/igem/BBa_I13205/annotation1043942/range1043942/1
component: BBa_E0030

https://synbiohub.org/public/igem/BBa_I13205/annotation1043942/range1043942/1
type: Range
persistent identity: range1043942
display id: range1043942
version: 1
direction: direction/forward
top level: BBa_I13205
start: 1185
end: 1907
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1043884
display id: annotation1043884
version: 1
top level: BBa_I13205
location: https://synbiohub.org/public/igem/BBa_I13205/annotation1043884/range1043884/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I13205/annotation1043884/range1043884/1
type: Range
persistent identity: range1043884
display id: range1043884
version: 1
direction: direction/forward
top level: BBa_I13205
start: 160
end: 171
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1043907
display id: annotation1043907
version: 1
top level: BBa_I13205
location: https://synbiohub.org/public/igem/BBa_I13205/annotation1043907/range1043907/1
component: BBa_B0010

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type: Range
persistent identity: range1043907
display id: range1043907
version: 1
direction: direction/forward
top level: BBa_I13205
start: 967
end: 1046
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1044000
display id: annotation1044000
version: 1
top level: BBa_I13205
location: https://synbiohub.org/public/igem/BBa_I13205/annotation1044000/range1044000/1
component: BBa_B0012

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type: Range
persistent identity: range1044000
display id: range1044000
version: 1
direction: direction/forward
top level: BBa_I13205
start: 4199
end: 4239
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1043947
display id: annotation1043947
version: 1
top level: BBa_I13205
location: https://synbiohub.org/public/igem/BBa_I13205/annotation1043947/range1043947/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I13205/annotation1043947/range1043947/1
type: Range
persistent identity: range1043947
display id: range1043947
version: 1
direction: direction/forward
top level: BBa_I13205
start: 1916
end: 1995
orientation: inline

BBa_K082006
type: ComponentDefinition
persistent identity: BBa K082006
display id: BBa_K082006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LuxR-G2F
creator: Yanting Xue , He Shen
created: 2008-10-27T12:00:00Z
modified: 2015-05-08T01:08:35Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _228_
m user id: 0
owner id: 1560
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: none
mutable notes: None
mutable provenance: None
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: BBa_K082006
sequence: https://synbiohub.org/public/igem/BBa_K082006_sequence/1

BBa_K082006
type: SequenceAnnotation
persistent identity: annotation1993281
display id: annotation1993281
version: 1
top level: BBa_K082006
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K082006/annotation1993281/range1993281/1

https://synbiohub.org/public/igem/BBa_K082006/annotation1993281/range1993281/1
type: Range
persistent identity: range1993281
display id: range1993281
version: 1
direction: direction/reverse
top level: BBa_K082006
start: 1
end: 753
orientation: inline

BBa_K332033
type: ComponentDefinition
persistent identity: BBa K332033
display id: BBa_K332033
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: 37℃ induced RBS + tetR + double terminator
creator: KUO, CHIA-CHUN
created: 2010-10-23T11:00:00Z
modified: 2015-05-08T01:12:05Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _452_
m user id: 0
owner id: 7059
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: It is a transcriptional unit including 37℃ induced RBS + tetR + double terminator. It can be added various promoter to adapt your conditions.
mutable notes: To regulate following genes by temperature. To maintain cells at 37?? C ↑ : the tetR protein be generated and repress Ptet's circuit. To release cells at 37?? C ↓ : the tetR protein not be generated and do not repress Ptet's circuit.
mutable provenance: the 37℃ induced RBS cames from iGEM08_TUDelft
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_C0040
component: BBa_J61048
component: BBa_K115002
sequence annotation: BBa_K115002
sequence annotation: BBa_C0040
sequence annotation: BBa_J61048
sequence: https://synbiohub.org/public/igem/BBa_K332033_sequence/1

BBa_C0040
type: Component
persistent identity: component2247615
display id: component2247615
version: 1
top level: BBa_K332033
access: public
definition: https://synbiohub.org/public/igem/BBa_C0040/1

BBa_J61048
type: Component
persistent identity: component2247616
display id: component2247616
version: 1
top level: BBa_K332033
access: public
definition: https://synbiohub.org/public/igem/BBa_J61048/1

BBa_K115002
type: Component
persistent identity: component2247611
display id: component2247611
version: 1
top level: BBa_K332033
access: public
definition: https://synbiohub.org/public/igem/BBa_K115002/1

BBa_K115002
type: SequenceAnnotation
persistent identity: annotation2247611
display id: annotation2247611
version: 1
top level: BBa_K332033
location: https://synbiohub.org/public/igem/BBa_K332033/annotation2247611/range2247611/1
component: BBa_K115002

https://synbiohub.org/public/igem/BBa_K332033/annotation2247611/range2247611/1
type: Range
persistent identity: range2247611
display id: range2247611
version: 1
direction: direction/reverse
top level: BBa_K332033
start: 1
end: 52
orientation: inline

BBa_C0040
type: SequenceAnnotation
persistent identity: annotation2247615
display id: annotation2247615
version: 1
top level: BBa_K332033
location: https://synbiohub.org/public/igem/BBa_K332033/annotation2247615/range2247615/1
component: BBa_C0040

https://synbiohub.org/public/igem/BBa_K332033/annotation2247615/range2247615/1
type: Range
persistent identity: range2247615
display id: range2247615
version: 1
direction: direction/reverse
top level: BBa_K332033
start: 59
end: 743
orientation: inline

BBa_J61048
type: SequenceAnnotation
persistent identity: annotation2247616
display id: annotation2247616
version: 1
top level: BBa_K332033
location: https://synbiohub.org/public/igem/BBa_K332033/annotation2247616/range2247616/1
component: BBa_J61048

https://synbiohub.org/public/igem/BBa_K332033/annotation2247616/range2247616/1
type: Range
persistent identity: range2247616
display id: range2247616
version: 1
direction: direction/reverse
top level: BBa_K332033
start: 752
end: 864
orientation: inline

BBa_K1520021
type: ComponentDefinition
persistent identity: BBa K1520021
display id: BBa_K1520021
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Pcons2-rbs-lacI-Ter-Plac-rbs-luxI-Ter-Pcons2-rbs-luxR-Ter-Prlux-rbs-rfp-Ter
creator: yue wu
created: 2014-09-21T11:00:00Z
modified: 2015-05-08T01:10:49Z
discontinued: false
dominant: false
group u list: _1902_
m user id: 0
owner id: 21318
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Plasmid in positive feedback verification: Pcons2-rbs-lacI-Ter-Plac-rbs-luxI-Ter-Pcons2-rbs-luxR-Ter-Prlux-rbs-rfp-Ter. It is a control plasmid for positive feedback circuit. It will produce rfp when there is enough IPTG.
mutable notes: None
mutable provenance: Pcons2, lacI, Plac, luxI, Pcons2, luxR, Prlux, rfp, Ter
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1520010
component: BBa_K1520020
sequence annotation: BBa_K1520020
sequence annotation: BBa_K1520010
sequence: https://synbiohub.org/public/igem/BBa_K1520021_sequence/1

BBa_K1520010
type: Component
persistent identity: component2385150
display id: component2385150
version: 1
top level: BBa_K1520021
access: public
definition: https://synbiohub.org/public/igem/BBa_K1520010/1

BBa_K1520020
type: Component
persistent identity: component2385142
display id: component2385142
version: 1
top level: BBa_K1520021
access: public
definition: BBa_K1520020

BBa_K1520020
type: SequenceAnnotation
persistent identity: annotation2385142
display id: annotation2385142
version: 1
top level: BBa_K1520021
location: https://synbiohub.org/public/igem/BBa_K1520021/annotation2385142/range2385142/1
component: BBa_K1520020

https://synbiohub.org/public/igem/BBa_K1520021/annotation2385142/range2385142/1
type: Range
persistent identity: range2385142
display id: range2385142
version: 1
direction: direction/reverse
top level: BBa_K1520021
start: 1
end: 3352
orientation: inline

BBa_K1520010
type: SequenceAnnotation
persistent identity: annotation2385150
display id: annotation2385150
version: 1
top level: BBa_K1520021
location: https://synbiohub.org/public/igem/BBa_K1520021/annotation2385150/range2385150/1
component: BBa_K1520010

https://synbiohub.org/public/igem/BBa_K1520021/annotation2385150/range2385150/1
type: Range
persistent identity: range2385150
display id: range2385150
version: 1
direction: direction/reverse
top level: BBa_K1520021
start: 3361
end: 4292
orientation: inline

BBa_J07013
type: ComponentDefinition
persistent identity: BBa J07013
display id: BBa_J07013
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: scFv anti-fluorescein [S101A]
creator: mit_igem_05
created: 2005-07-07T11:00:00Z
modified: 2015-08-31T04:08:19Z
discontinued: false
dominant: false
experience: experience/None
group u list: _21_
m user id: 0
owner id: 363
owning group id: 21
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Ser H101 Ala the mid-strength of the 3 piece anti-flour escein scFv sequence set.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Signalling
role: SO:0000804
sequence annotation: VH
sequence annotation: VL
sequence annotation: CDR1 VH
sequence annotation: CDR3 VH
sequence annotation: CDR1 VL
sequence annotation: CDR2 VL
sequence annotation: S - A
sequence annotation: CDR3 VL
sequence annotation: linker
sequence annotation: CDR2 VH
sequence: https://synbiohub.org/public/igem/BBa_J07013_sequence/1

VH
type: SequenceAnnotation
persistent identity: annotation1582310
display id: annotation1582310
version: 1
top level: BBa_J07013
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J07013/annotation1582310/range1582310/1

https://synbiohub.org/public/igem/BBa_J07013/annotation1582310/range1582310/1
type: Range
persistent identity: range1582310
display id: range1582310
version: 1
direction: direction/forward
top level: BBa_J07013
start: 412
end: 762
orientation: inline

VL
type: SequenceAnnotation
persistent identity: annotation1582309
display id: annotation1582309
version: 1
top level: BBa_J07013
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J07013/annotation1582309/range1582309/1

https://synbiohub.org/public/igem/BBa_J07013/annotation1582309/range1582309/1
type: Range
persistent identity: range1582309
display id: range1582309
version: 1
direction: direction/forward
top level: BBa_J07013
start: 1
end: 336
orientation: inline

CDR1 VH
type: SequenceAnnotation
persistent identity: annotation1582303
display id: annotation1582303
version: 1
top level: BBa_J07013
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J07013/annotation1582303/range1582303/1

https://synbiohub.org/public/igem/BBa_J07013/annotation1582303/range1582303/1
type: Range
persistent identity: range1582303
display id: range1582303
version: 1
direction: direction/forward
top level: BBa_J07013
start: 502
end: 513
orientation: inline

CDR3 VH
type: SequenceAnnotation
persistent identity: annotation1582308
display id: annotation1582308
version: 1
top level: BBa_J07013
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J07013/annotation1582308/range1582308/1

https://synbiohub.org/public/igem/BBa_J07013/annotation1582308/range1582308/1
type: Range
persistent identity: range1582308
display id: range1582308
version: 1
direction: direction/forward
top level: BBa_J07013
start: 711
end: 732
orientation: inline

CDR1 VL
type: SequenceAnnotation
persistent identity: annotation1582290
display id: annotation1582290
version: 1
top level: BBa_J07013
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J07013/annotation1582290/range1582290/1

https://synbiohub.org/public/igem/BBa_J07013/annotation1582290/range1582290/1
type: Range
persistent identity: range1582290
display id: range1582290
version: 1
direction: direction/forward
top level: BBa_J07013
start: 61
end: 120
orientation: inline

CDR2 VL
type: SequenceAnnotation
persistent identity: annotation1582296
display id: annotation1582296
version: 1
top level: BBa_J07013
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J07013/annotation1582296/range1582296/1

https://synbiohub.org/public/igem/BBa_J07013/annotation1582296/range1582296/1
type: Range
persistent identity: range1582296
display id: range1582296
version: 1
direction: direction/forward
top level: BBa_J07013
start: 163
end: 183
orientation: inline

S - A
type: SequenceAnnotation
persistent identity: annotation1582289
display id: annotation1582289
version: 1
top level: BBa_J07013
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_J07013/annotation1582289/range1582289/1

https://synbiohub.org/public/igem/BBa_J07013/annotation1582289/range1582289/1
type: Range
persistent identity: range1582289
display id: range1582289
version: 1
direction: direction/forward
top level: BBa_J07013
start: 712
end: 714
orientation: inline

CDR3 VL
type: SequenceAnnotation
persistent identity: annotation1582301
display id: annotation1582301
version: 1
top level: BBa_J07013
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J07013/annotation1582301/range1582301/1

https://synbiohub.org/public/igem/BBa_J07013/annotation1582301/range1582301/1
type: Range
persistent identity: range1582301
display id: range1582301
version: 1
direction: direction/forward
top level: BBa_J07013
start: 280
end: 306
orientation: inline

linker
type: SequenceAnnotation
persistent identity: annotation1582302
display id: annotation1582302
version: 1
top level: BBa_J07013
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J07013/annotation1582302/range1582302/1

https://synbiohub.org/public/igem/BBa_J07013/annotation1582302/range1582302/1
type: Range
persistent identity: range1582302
display id: range1582302
version: 1
direction: direction/forward
top level: BBa_J07013
start: 337
end: 411
orientation: inline

CDR2 VH
type: SequenceAnnotation
persistent identity: annotation1582304
display id: annotation1582304
version: 1
top level: BBa_J07013
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J07013/annotation1582304/range1582304/1

https://synbiohub.org/public/igem/BBa_J07013/annotation1582304/range1582304/1
type: Range
persistent identity: range1582304
display id: range1582304
version: 1
direction: direction/forward
top level: BBa_J07013
start: 559
end: 615
orientation: inline

cinI
type: ComponentDefinition
persistent identity: BBa C0076
display id: BBa_C0076
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: autoinducer synthetase
creator: crackdots
created: 2004-01-27T12:00:00Z
modified: 2015-08-31T04:07:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: cinI codes for an autoinducer synthetase which utilizes SAM to make O3-C14:1-HSL. In complex with O3-C14:1-HSL, CinR (BBa_C0077) binds to the Cin promoter (BBa_R0077) and activates transcription.
mutable notes: The regulatory locus cinRI in Rhizobium leguminosarum conrols a network of quorum-sensing loci Lithgow, JK; Wilkinson, A; Hardman, A; Rodelas, B; Wisniewski-Dye, F; Williams, P; Downie, AJ MOL. MICROBIOLOGY 37(1): 81-97, 2000 Change log: original STOP: tga -> tAaTAA
mutable provenance: Rhizobium leguminosarum
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Help:Barcodes
sequence annotation: LVA
sequence annotation: CinI
sequence: https://synbiohub.org/public/igem/BBa_C0076_sequence/1

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2214001
display id: annotation2214001
version: 1
top level: cinI
role: SO:0000807
role: feature/barcode
location: https://synbiohub.org/public/igem/BBa_C0076/annotation2214001/range2214001/1

https://synbiohub.org/public/igem/BBa_C0076/annotation2214001/range2214001/1
type: Range
persistent identity: range2214001
display id: range2214001
version: 1
direction: direction/forward
top level: cinI
start: 703
end: 727
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation306586
display id: annotation306586
version: 1
top level: cinI
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_C0076/annotation306586/range306586/1

https://synbiohub.org/public/igem/BBa_C0076/annotation306586/range306586/1
type: Range
persistent identity: range306586
display id: range306586
version: 1
direction: direction/forward
top level: cinI
start: 664
end: 696
orientation: inline

CinI
type: SequenceAnnotation
persistent identity: annotation301035
display id: annotation301035
version: 1
top level: cinI
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_C0076/annotation301035/range301035/1

https://synbiohub.org/public/igem/BBa_C0076/annotation301035/range301035/1
type: Range
persistent identity: range301035
display id: range301035
version: 1
direction: direction/forward
top level: cinI
start: 1
end: 663
orientation: inline

BBa_K266002
type: ComponentDefinition
persistent identity: BBa K266002
display id: BBa_K266002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LasR + Term
creator: UNAM FCIENCIAS-CINVESTAV IRA
created: 2009-10-12T11:00:00Z
modified: 2015-05-08T01:11:43Z
discontinued: false
dominant: false
experience: experience/None
group u list: _363_
m user id: 0
owner id: 1955
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: LasR coding region and a double terminator
mutable notes: None
mutable provenance: The biobricks BBa_C0079 and BBa_B0015 where used for this biobrick.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_C0079
component: BBa_B0015
sequence annotation: BBa_C0079
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_K266002_sequence/1

BBa_C0079
type: Component
persistent identity: component2246664
display id: component2246664
version: 1
top level: BBa_K266002
access: public
definition: lasr

BBa_B0015
type: Component
persistent identity: component2246671
display id: component2246671
version: 1
top level: BBa_K266002
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_C0079
type: SequenceAnnotation
persistent identity: annotation2246664
display id: annotation2246664
version: 1
top level: BBa_K266002
location: https://synbiohub.org/public/igem/BBa_K266002/annotation2246664/range2246664/1
component: BBa_C0079

https://synbiohub.org/public/igem/BBa_K266002/annotation2246664/range2246664/1
type: Range
persistent identity: range2246664
display id: range2246664
version: 1
direction: direction/reverse
top level: BBa_K266002
start: 1
end: 781
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2246671
display id: annotation2246671
version: 1
top level: BBa_K266002
location: https://synbiohub.org/public/igem/BBa_K266002/annotation2246671/range2246671/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K266002/annotation2246671/range2246671/1
type: Range
persistent identity: range2246671
display id: range2246671
version: 1
direction: direction/reverse
top level: BBa_K266002
start: 790
end: 918
orientation: inline

BBa_K819015
type: ComponentDefinition
persistent identity: BBa K819015
display id: BBa_K819015
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Luminesensor repressible RecA408 promoter+CheZ
creator: YU Zhou
created: 2012-09-15T11:00:00Z
modified: 2015-05-08T01:13:29Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1077_
m user id: 0
owner id: 13785
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: CheZ coding sequence ligated to the Luminesensor repressible recA408 promoter
mutable notes: CheZ coding sequence ligated to the Luminesensor repressible recA408 promoter.
mutable provenance: CheZ is got from E.Coli strain MG1655 by colony PCR
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Signalling
role: SO:0000804
sequence annotation: B0034
sequence annotation: CheZ
sequence annotation: RecA408 Promoter
sequence: https://synbiohub.org/public/igem/BBa_K819015_sequence/1

B0034
type: SequenceAnnotation
persistent identity: annotation2197269
display id: annotation2197269
version: 1
top level: BBa_K819015
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K819015/annotation2197269/range2197269/1

https://synbiohub.org/public/igem/BBa_K819015/annotation2197269/range2197269/1
type: Range
persistent identity: range2197269
display id: range2197269
version: 1
direction: direction/forward
top level: BBa_K819015
start: 90
end: 101
orientation: inline

CheZ
type: SequenceAnnotation
persistent identity: annotation2197270
display id: annotation2197270
version: 1
top level: BBa_K819015
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K819015/annotation2197270/range2197270/1

https://synbiohub.org/public/igem/BBa_K819015/annotation2197270/range2197270/1
type: Range
persistent identity: range2197270
display id: range2197270
version: 1
direction: direction/forward
top level: BBa_K819015
start: 108
end: 752
orientation: inline

RecA408 Promoter
type: SequenceAnnotation
persistent identity: annotation2197268
display id: annotation2197268
version: 1
top level: BBa_K819015
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K819015/annotation2197268/range2197268/1

https://synbiohub.org/public/igem/BBa_K819015/annotation2197268/range2197268/1
type: Range
persistent identity: range2197268
display id: range2197268
version: 1
direction: direction/forward
top level: BBa_K819015
start: 1
end: 81
orientation: inline

BBa_K082035
type: ComponentDefinition
persistent identity: BBa K082035
display id: BBa_K082035
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RhlI generator
creator: Zhen Xu , Binjie Xu
created: 2008-10-27T12:00:00Z
modified: 2015-05-08T01:08:36Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _228_
m user id: 0
owner id: 1560
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: none
mutable notes: none
mutable provenance: none
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0034
component: BBa_R0040
component: BBa_B0015
component: BBa_C0070
sequence annotation: BBa_B0015
sequence annotation: BBa_R0040
sequence annotation: BBa_B0034
sequence annotation: BBa_C0070
sequence: https://synbiohub.org/public/igem/BBa_K082035_sequence/1

BBa_B0034
type: Component
persistent identity: component2254788
display id: component2254788
version: 1
top level: BBa_K082035
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_R0040
type: Component
persistent identity: component2254782
display id: component2254782
version: 1
top level: BBa_K082035
access: public
definition: https://synbiohub.org/public/igem/BBa_R0040/1

BBa_B0015
type: Component
persistent identity: component2254799
display id: component2254799
version: 1
top level: BBa_K082035
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_C0070
type: Component
persistent identity: component2254792
display id: component2254792
version: 1
top level: BBa_K082035
access: public
definition: rhII

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2254799
display id: annotation2254799
version: 1
top level: BBa_K082035
location: https://synbiohub.org/public/igem/BBa_K082035/annotation2254799/range2254799/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K082035/annotation2254799/range2254799/1
type: Range
persistent identity: range2254799
display id: range2254799
version: 1
direction: direction/reverse
top level: BBa_K082035
start: 756
end: 884
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2254782
display id: annotation2254782
version: 1
top level: BBa_K082035
location: https://synbiohub.org/public/igem/BBa_K082035/annotation2254782/range2254782/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_K082035/annotation2254782/range2254782/1
type: Range
persistent identity: range2254782
display id: range2254782
version: 1
direction: direction/forward
top level: BBa_K082035
start: 1
end: 54
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2254788
display id: annotation2254788
version: 1
top level: BBa_K082035
location: https://synbiohub.org/public/igem/BBa_K082035/annotation2254788/range2254788/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K082035/annotation2254788/range2254788/1
type: Range
persistent identity: range2254788
display id: range2254788
version: 1
direction: direction/reverse
top level: BBa_K082035
start: 63
end: 74
orientation: inline

BBa_C0070
type: SequenceAnnotation
persistent identity: annotation2254792
display id: annotation2254792
version: 1
top level: BBa_K082035
location: https://synbiohub.org/public/igem/BBa_K082035/annotation2254792/range2254792/1
component: BBa_C0070

https://synbiohub.org/public/igem/BBa_K082035/annotation2254792/range2254792/1
type: Range
persistent identity: range2254792
display id: range2254792
version: 1
direction: direction/reverse
top level: BBa_K082035
start: 81
end: 747
orientation: inline

agrD
type: ComponentDefinition
persistent identity: BBa K090601
display id: BBa_K090601
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: AgrD: AGR System AIP Precursor
creator: Daniel Goodman
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:08:37Z
discontinued: false
dominant: false
experience: experience/None
group u list: _188_
m user id: 0
owner id: 3501
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This is the coding sequence for AgrD, the gene encoding the peptide precursor to Autoinducing Peptide, AIP. The AgrD protein product is co-translationally exported, cleaved, and circularized via the AgrB membrane exporter. It then binds to the extracellular domain of AgrC initiating a signalling cascade within the cell.
mutable notes: We wanted to use the coding sequence without the E. coli RBS present in BBa_I746001 and so PCRed this part out. It can be used with any RBS, including gram-positive compatible ones such as BBa_K090505.
mutable provenance: This part was PCRed out of BBa_I746001.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K090601_sequence/1

BBa_K082023
type: ComponentDefinition
persistent identity: BBa K082023
display id: BBa_K082023
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: B0034-C0070-B0015
creator: Zhen Xu, Binjie Xu
created: 2008-10-28T12:00:00Z
modified: 2015-05-08T01:08:35Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _228_
m user id: 0
owner id: 1560
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: none
mutable notes: none
mutable provenance: none
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0015
component: BBa_C0070
component: BBa_B0034
sequence annotation: BBa_B0034
sequence annotation: BBa_B0015
sequence annotation: BBa_C0070
sequence: https://synbiohub.org/public/igem/BBa_K082023_sequence/1

BBa_B0015
type: Component
persistent identity: component2249414
display id: component2249414
version: 1
top level: BBa_K082023
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_C0070
type: Component
persistent identity: component2249407
display id: component2249407
version: 1
top level: BBa_K082023
access: public
definition: rhII

BBa_B0034
type: Component
persistent identity: component2249403
display id: component2249403
version: 1
top level: BBa_K082023
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2249403
display id: annotation2249403
version: 1
top level: BBa_K082023
location: https://synbiohub.org/public/igem/BBa_K082023/annotation2249403/range2249403/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K082023/annotation2249403/range2249403/1
type: Range
persistent identity: range2249403
display id: range2249403
version: 1
direction: direction/reverse
top level: BBa_K082023
start: 1
end: 12
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2249414
display id: annotation2249414
version: 1
top level: BBa_K082023
location: https://synbiohub.org/public/igem/BBa_K082023/annotation2249414/range2249414/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K082023/annotation2249414/range2249414/1
type: Range
persistent identity: range2249414
display id: range2249414
version: 1
direction: direction/reverse
top level: BBa_K082023
start: 694
end: 822
orientation: inline

BBa_C0070
type: SequenceAnnotation
persistent identity: annotation2249407
display id: annotation2249407
version: 1
top level: BBa_K082023
location: https://synbiohub.org/public/igem/BBa_K082023/annotation2249407/range2249407/1
component: BBa_C0070

https://synbiohub.org/public/igem/BBa_K082023/annotation2249407/range2249407/1
type: Range
persistent identity: range2249407
display id: range2249407
version: 1
direction: direction/reverse
top level: BBa_K082023
start: 19
end: 685
orientation: inline

BBa_F2621
type: ComponentDefinition
persistent identity: BBa F2621
display id: BBa_F2621
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: 3OC6HSL Receiver Device
creator: Barry Canton
created: 2004-08-08T11:00:00Z
modified: 2015-08-31T04:07:27Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _11_6_
m user id: 0
owner id: 135
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This device senses the 3OC6HSL concentration in the media and produces output PoPS according to the transfer function given below. LuxR production is controlled by the LuxR controllable R0063.
mutable provenance: Formerly I13260
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Signalling
role: SO:0000804
component: BBa_B0034
component: BBa_B0010
component: BBa_C0062
component: BBa_R0063
component: BBa_B0012
component: BBa_R0062
sequence annotation: BBa_B0012
sequence annotation: BBa_C0062
sequence annotation: BBa_R0062
sequence annotation: BBa_R0063
sequence annotation: BBa_B0010
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_F2621_sequence/1

BBa_B0034
type: Component
persistent identity: component1484739
display id: component1484739
version: 1
top level: BBa_F2621
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0010
type: Component
persistent identity: component1484762
display id: component1484762
version: 1
top level: BBa_F2621
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_C0062
type: Component
persistent identity: component1484754
display id: component1484754
version: 1
top level: BBa_F2621
access: public
definition: luxr

BBa_R0063
type: Component
persistent identity: component1484730
display id: component1484730
version: 1
top level: BBa_F2621
access: public
definition: https://synbiohub.org/public/igem/BBa_R0063/1

BBa_B0012
type: Component
persistent identity: component1484772
display id: component1484772
version: 1
top level: BBa_F2621
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_R0062
type: Component
persistent identity: component1484787
display id: component1484787
version: 1
top level: BBa_F2621
access: public
definition: https://synbiohub.org/public/igem/BBa_R0062/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1484772
display id: annotation1484772
version: 1
top level: BBa_F2621
location: https://synbiohub.org/public/igem/BBa_F2621/annotation1484772/range1484772/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_F2621/annotation1484772/range1484772/1
type: Range
persistent identity: range1484772
display id: range1484772
version: 1
direction: direction/forward
top level: BBa_F2621
start: 1055
end: 1095
orientation: inline

BBa_C0062
type: SequenceAnnotation
persistent identity: annotation1484754
display id: annotation1484754
version: 1
top level: BBa_F2621
location: https://synbiohub.org/public/igem/BBa_F2621/annotation1484754/range1484754/1
component: BBa_C0062

https://synbiohub.org/public/igem/BBa_F2621/annotation1484754/range1484754/1
type: Range
persistent identity: range1484754
display id: range1484754
version: 1
direction: direction/forward
top level: BBa_F2621
start: 178
end: 933
orientation: inline

BBa_R0062
type: SequenceAnnotation
persistent identity: annotation1484787
display id: annotation1484787
version: 1
top level: BBa_F2621
location: https://synbiohub.org/public/igem/BBa_F2621/annotation1484787/range1484787/1
component: BBa_R0062

https://synbiohub.org/public/igem/BBa_F2621/annotation1484787/range1484787/1
type: Range
persistent identity: range1484787
display id: range1484787
version: 1
direction: direction/forward
top level: BBa_F2621
start: 1104
end: 1158
orientation: inline

BBa_R0063
type: SequenceAnnotation
persistent identity: annotation1484730
display id: annotation1484730
version: 1
top level: BBa_F2621
location: https://synbiohub.org/public/igem/BBa_F2621/annotation1484730/range1484730/1
component: BBa_R0063

https://synbiohub.org/public/igem/BBa_F2621/annotation1484730/range1484730/1
type: Range
persistent identity: range1484730
display id: range1484730
version: 1
direction: direction/forward
top level: BBa_F2621
start: 1
end: 151
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1484762
display id: annotation1484762
version: 1
top level: BBa_F2621
location: https://synbiohub.org/public/igem/BBa_F2621/annotation1484762/range1484762/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_F2621/annotation1484762/range1484762/1
type: Range
persistent identity: range1484762
display id: range1484762
version: 1
direction: direction/forward
top level: BBa_F2621
start: 967
end: 1046
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1484739
display id: annotation1484739
version: 1
top level: BBa_F2621
location: https://synbiohub.org/public/igem/BBa_F2621/annotation1484739/range1484739/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_F2621/annotation1484739/range1484739/1
type: Range
persistent identity: range1484739
display id: range1484739
version: 1
direction: direction/forward
top level: BBa_F2621
start: 160
end: 171
orientation: inline

BBa_I13262
type: ComponentDefinition
persistent identity: BBa I13262
display id: BBa_I13262
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PoPS->PoPS Amplifier Controlled by 3OC6HSL
creator: Barry Canton
created: 2004-08-08T11:00:00Z
modified: 2015-08-31T04:07:33Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _11_6_
m user id: 0
owner id: 135
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: LuxR protein generator upstream of pLuxR. Useful device for cell-cell signaling. Expected to require HSL for maximum PoPS out. Putting this device under the control of a promoter produces a receiver device
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Signalling
role: SO:0000804
component: BBa_C0062
component: BBa_B0012
component: BBa_R0062
component: BBa_B0010
component: BBa_B0034
sequence annotation: BBa_B0034
sequence annotation: BBa_B0012
sequence annotation: BBa_R0062
sequence annotation: BBa_C0062
sequence annotation: BBa_B0010
sequence: https://synbiohub.org/public/igem/BBa_I13262_sequence/1

BBa_C0062
type: Component
persistent identity: component1035360
display id: component1035360
version: 1
top level: BBa_I13262
access: public
definition: luxr

BBa_B0012
type: Component
persistent identity: component1035378
display id: component1035378
version: 1
top level: BBa_I13262
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_R0062
type: Component
persistent identity: component1035393
display id: component1035393
version: 1
top level: BBa_I13262
access: public
definition: https://synbiohub.org/public/igem/BBa_R0062/1

BBa_B0010
type: Component
persistent identity: component1035368
display id: component1035368
version: 1
top level: BBa_I13262
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0034
type: Component
persistent identity: component1035345
display id: component1035345
version: 1
top level: BBa_I13262
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1035345
display id: annotation1035345
version: 1
top level: BBa_I13262
location: https://synbiohub.org/public/igem/BBa_I13262/annotation1035345/range1035345/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I13262/annotation1035345/range1035345/1
type: Range
persistent identity: range1035345
display id: range1035345
version: 1
direction: direction/forward
top level: BBa_I13262
start: 1
end: 12
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1035378
display id: annotation1035378
version: 1
top level: BBa_I13262
location: https://synbiohub.org/public/igem/BBa_I13262/annotation1035378/range1035378/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I13262/annotation1035378/range1035378/1
type: Range
persistent identity: range1035378
display id: range1035378
version: 1
direction: direction/forward
top level: BBa_I13262
start: 896
end: 936
orientation: inline

BBa_R0062
type: SequenceAnnotation
persistent identity: annotation1035393
display id: annotation1035393
version: 1
top level: BBa_I13262
location: https://synbiohub.org/public/igem/BBa_I13262/annotation1035393/range1035393/1
component: BBa_R0062

https://synbiohub.org/public/igem/BBa_I13262/annotation1035393/range1035393/1
type: Range
persistent identity: range1035393
display id: range1035393
version: 1
direction: direction/forward
top level: BBa_I13262
start: 945
end: 999
orientation: inline

BBa_C0062
type: SequenceAnnotation
persistent identity: annotation1035360
display id: annotation1035360
version: 1
top level: BBa_I13262
location: https://synbiohub.org/public/igem/BBa_I13262/annotation1035360/range1035360/1
component: BBa_C0062

https://synbiohub.org/public/igem/BBa_I13262/annotation1035360/range1035360/1
type: Range
persistent identity: range1035360
display id: range1035360
version: 1
direction: direction/forward
top level: BBa_I13262
start: 19
end: 774
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1035368
display id: annotation1035368
version: 1
top level: BBa_I13262
location: https://synbiohub.org/public/igem/BBa_I13262/annotation1035368/range1035368/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I13262/annotation1035368/range1035368/1
type: Range
persistent identity: range1035368
display id: range1035368
version: 1
direction: direction/forward
top level: BBa_I13262
start: 808
end: 887
orientation: inline

BBa_K546000
type: ComponentDefinition
persistent identity: BBa K546000
display id: BBa_K546000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Lux pL controlled LuxR with lux pR autoinducing LuxI(lva tag)- AHL.
creator: M. Hesselman
created: 2011-09-13T11:00:00Z
modified: 2015-05-08T01:12:39Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _713_
m user id: 0
owner id: 9055
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: The F2621 BioBrick device contains a lux pL regulated luxR repressor/activator and a lux pR promoter that is linked to an 3OC6HSL, an AHL autoinducer molecule, synthetase. The lux pL promoter yields a weak constitutive downstream expression. When LuxR and 3OC6HSL are produced, the lux pL promoter, however, will be repressed by a complex of the two. Complementary to the lux pL promoter, the lux pR promoter with a complex of luxR and 3OC6HSL will enable enlarged activation of the downstream synthetase gene, thus increase the concentration 3OC6HSL.
mutable notes: The original R0063 BioBrick part (promoter) of the F2621 BioBrick device turned out to be absent in our construct, as was shown by a sequence analysis. To increase the transcription activation there has been put an extra R0063 BioBrick part promoter in front of the F2621 BioBrick device. The resulting construct worked a lot better than the original F2621 - K082014 BioBrick system.
mutable provenance: The lux p promoters and the autoinducer producing enzyme originate from the quorum sensing ability of ''Vibrio fischeri''.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Signalling
role: SO:0000804
component: BBa_F2621
component: BBa_K082014
sequence annotation: BBa_F2621
sequence annotation: BBa_K082014
sequence: https://synbiohub.org/public/igem/BBa_K546000_sequence/1

BBa_F2621
type: Component
persistent identity: component2289549
display id: component2289549
version: 1
top level: BBa_K546000
access: public
definition: BBa_F2621

BBa_K082014
type: Component
persistent identity: component2289564
display id: component2289564
version: 1
top level: BBa_K546000
access: public
definition: https://synbiohub.org/public/igem/BBa_K082014/1

BBa_F2621
type: SequenceAnnotation
persistent identity: annotation2289549
display id: annotation2289549
version: 1
top level: BBa_K546000
location: https://synbiohub.org/public/igem/BBa_K546000/annotation2289549/range2289549/1
component: BBa_F2621

https://synbiohub.org/public/igem/BBa_K546000/annotation2289549/range2289549/1
type: Range
persistent identity: range2289549
display id: range2289549
version: 1
direction: direction/reverse
top level: BBa_K546000
start: 1
end: 1158
orientation: inline

BBa_K082014
type: SequenceAnnotation
persistent identity: annotation2289564
display id: annotation2289564
version: 1
top level: BBa_K546000
location: https://synbiohub.org/public/igem/BBa_K546000/annotation2289564/range2289564/1
component: BBa_K082014

https://synbiohub.org/public/igem/BBa_K546000/annotation2289564/range2289564/1
type: Range
persistent identity: range2289564
display id: range2289564
version: 1
direction: direction/reverse
top level: BBa_K546000
start: 1167
end: 1964
orientation: inline

BBa_K1315003
type: ComponentDefinition
persistent identity: BBa K1315003
display id: BBa_K1315003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: The rpfG gene from Xanthomonas campestris
creator: Dimitrios Michailidis
created: 2014-09-24T11:00:00Z
modified: 2015-05-08T01:09:48Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1690_
m user id: 0
owner id: 21812
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This is the coding sequence for the RpfG(regulation of pathogenicity factor G) protein. It is the regulator in the two-component signal transduction system of Xanthomonas campestris pv. campestris that is activated by the sensor kinase RpfC(Bba_K1315002) in the presence of DSF(diffusible signal factor).
mutable notes: The sequence had 1 EcoRI site that was mutated out by site-directed mutagenesis.
mutable provenance: The source of this part is Xanthomonas campestris pv. campestris(Xcc) genomic DNA which was provided by the Robert Ryan lab in the Molecular Microbiology department of the University of Dundee, UK.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: RpfG
sequence: https://synbiohub.org/public/igem/BBa_K1315003_sequence/1

RpfG
type: SequenceAnnotation
persistent identity: annotation2386405
display id: annotation2386405
version: 1
top level: BBa_K1315003
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1315003/annotation2386405/range2386405/1

https://synbiohub.org/public/igem/BBa_K1315003/annotation2386405/range2386405/1
type: Range
persistent identity: range2386405
display id: range2386405
version: 1
direction: direction/forward
top level: BBa_K1315003
start: 1
end: 1140
orientation: inline

BBa_I1467
type: ComponentDefinition
persistent identity: BBa I1467
display id: BBa_I1467
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: AI-1 protein generator (LVA-)
creator: labnoa
created: 2005-07-10T11:00:00Z
modified: 2015-08-31T04:07:38Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_
m user id: 0
owner id: 371
owning group id: 11
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Signalling
role: SO:0000804
component: BBa_B0012
component: BBa_C0179
component: BBa_B0010
component: BBa_B0034
sequence annotation: BBa_C0179
sequence annotation: BBa_B0012
sequence annotation: BBa_B0034
sequence annotation: BBa_B0010
sequence: https://synbiohub.org/public/igem/BBa_I1467_sequence/1

BBa_B0012
type: Component
persistent identity: component1574195
display id: component1574195
version: 1
top level: BBa_I1467
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_C0179
type: Component
persistent identity: component1574180
display id: component1574180
version: 1
top level: BBa_I1467
access: public
definition: lasR

BBa_B0010
type: Component
persistent identity: component1574185
display id: component1574185
version: 1
top level: BBa_I1467
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0034
type: Component
persistent identity: component1574178
display id: component1574178
version: 1
top level: BBa_I1467
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_C0179
type: SequenceAnnotation
persistent identity: annotation1574180
display id: annotation1574180
version: 1
top level: BBa_I1467
location: https://synbiohub.org/public/igem/BBa_I1467/annotation1574180/range1574180/1
component: BBa_C0179

https://synbiohub.org/public/igem/BBa_I1467/annotation1574180/range1574180/1
type: Range
persistent identity: range1574180
display id: range1574180
version: 1
direction: direction/forward
top level: BBa_I1467
start: 19
end: 741
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1574195
display id: annotation1574195
version: 1
top level: BBa_I1467
location: https://synbiohub.org/public/igem/BBa_I1467/annotation1574195/range1574195/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I1467/annotation1574195/range1574195/1
type: Range
persistent identity: range1574195
display id: range1574195
version: 1
direction: direction/forward
top level: BBa_I1467
start: 838
end: 878
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1574178
display id: annotation1574178
version: 1
top level: BBa_I1467
location: https://synbiohub.org/public/igem/BBa_I1467/annotation1574178/range1574178/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I1467/annotation1574178/range1574178/1
type: Range
persistent identity: range1574178
display id: range1574178
version: 1
direction: direction/forward
top level: BBa_I1467
start: 1
end: 12
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1574185
display id: annotation1574185
version: 1
top level: BBa_I1467
location: https://synbiohub.org/public/igem/BBa_I1467/annotation1574185/range1574185/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I1467/annotation1574185/range1574185/1
type: Range
persistent identity: range1574185
display id: range1574185
version: 1
direction: direction/forward
top level: BBa_I1467
start: 750
end: 829
orientation: inline

BBa_J07014
type: ComponentDefinition
persistent identity: BBa J07014
display id: BBa_J07014
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: scFv anti-fluorescein [4M5.3]
creator: mit_igem_2005
created: 2005-07-07T11:00:00Z
modified: 2015-08-31T04:08:19Z
discontinued: false
dominant: false
experience: experience/None
group u list: _21_
m user id: 0
owner id: 363
owning group id: 21
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: single chain Antibody fragment selected to bind to fluorescein molecules. This scFv is the strongest of the three specified sequences.
mutable provenance: see J07013.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Signalling
role: SO:0000804
sequence annotation: CDR1 VH
sequence annotation: linker
sequence annotation: P-A
sequence annotation: CDR2 VL
sequence annotation: M-T
sequence annotation: VH
sequence annotation: CDR3 VL
sequence annotation: S-A
sequence annotation: D-G
sequence annotation: CDR1 VL
sequence annotation: CDR2 VH
sequence annotation: F-V
sequence annotation: CDR3 VH
sequence annotation: S-N
sequence annotation: I-F
sequence annotation: S-G
sequence annotation: R-G
sequence annotation: VL
sequence annotation: D-E
sequence annotation: D-H
sequence annotation: A-T
sequence annotation: W-L
sequence annotation: Y-S
sequence: https://synbiohub.org/public/igem/BBa_J07014_sequence/1

CDR1 VH
type: SequenceAnnotation
persistent identity: annotation1582328
display id: annotation1582328
version: 1
top level: BBa_J07014
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J07014/annotation1582328/range1582328/1

https://synbiohub.org/public/igem/BBa_J07014/annotation1582328/range1582328/1
type: Range
persistent identity: range1582328
display id: range1582328
version: 1
direction: direction/forward
top level: BBa_J07014
start: 502
end: 513
orientation: inline

linker
type: SequenceAnnotation
persistent identity: annotation1582325
display id: annotation1582325
version: 1
top level: BBa_J07014
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J07014/annotation1582325/range1582325/1

https://synbiohub.org/public/igem/BBa_J07014/annotation1582325/range1582325/1
type: Range
persistent identity: range1582325
display id: range1582325
version: 1
direction: direction/forward
top level: BBa_J07014
start: 337
end: 411
orientation: inline

P-A
type: SequenceAnnotation
persistent identity: annotation1583287
display id: annotation1583287
version: 1
top level: BBa_J07014
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_J07014/annotation1583287/range1583287/1

https://synbiohub.org/public/igem/BBa_J07014/annotation1583287/range1583287/1
type: Range
persistent identity: range1583287
display id: range1583287
version: 1
direction: direction/forward
top level: BBa_J07014
start: 460
end: 462
orientation: inline

CDR2 VL
type: SequenceAnnotation
persistent identity: annotation1582323
display id: annotation1582323
version: 1
top level: BBa_J07014
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J07014/annotation1582323/range1582323/1

https://synbiohub.org/public/igem/BBa_J07014/annotation1582323/range1582323/1
type: Range
persistent identity: range1582323
display id: range1582323
version: 1
direction: direction/forward
top level: BBa_J07014
start: 163
end: 183
orientation: inline

M-T
type: SequenceAnnotation
persistent identity: annotation1583295
display id: annotation1583295
version: 1
top level: BBa_J07014
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_J07014/annotation1583295/range1583295/1

https://synbiohub.org/public/igem/BBa_J07014/annotation1583295/range1583295/1
type: Range
persistent identity: range1583295
display id: range1583295
version: 1
direction: direction/forward
top level: BBa_J07014
start: 688
end: 690
orientation: inline

VH
type: SequenceAnnotation
persistent identity: annotation1582336
display id: annotation1582336
version: 1
top level: BBa_J07014
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J07014/annotation1582336/range1582336/1

https://synbiohub.org/public/igem/BBa_J07014/annotation1582336/range1582336/1
type: Range
persistent identity: range1582336
display id: range1582336
version: 1
direction: direction/forward
top level: BBa_J07014
start: 412
end: 762
orientation: inline

CDR3 VL
type: SequenceAnnotation
persistent identity: annotation1582324
display id: annotation1582324
version: 1
top level: BBa_J07014
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J07014/annotation1582324/range1582324/1

https://synbiohub.org/public/igem/BBa_J07014/annotation1582324/range1582324/1
type: Range
persistent identity: range1582324
display id: range1582324
version: 1
direction: direction/forward
top level: BBa_J07014
start: 280
end: 306
orientation: inline

S-A
type: SequenceAnnotation
persistent identity: annotation1583296
display id: annotation1583296
version: 1
top level: BBa_J07014
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_J07014/annotation1583296/range1583296/1

https://synbiohub.org/public/igem/BBa_J07014/annotation1583296/range1583296/1
type: Range
persistent identity: range1583296
display id: range1583296
version: 1
direction: direction/forward
top level: BBa_J07014
start: 712
end: 714
orientation: inline

D-G
type: SequenceAnnotation
persistent identity: annotation1583279
display id: annotation1583279
version: 1
top level: BBa_J07014
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_J07014/annotation1583279/range1583279/1

https://synbiohub.org/public/igem/BBa_J07014/annotation1583279/range1583279/1
type: Range
persistent identity: range1583279
display id: range1583279
version: 1
direction: direction/forward
top level: BBa_J07014
start: 412
end: 414
orientation: inline

CDR1 VL
type: SequenceAnnotation
persistent identity: annotation1582322
display id: annotation1582322
version: 1
top level: BBa_J07014
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J07014/annotation1582322/range1582322/1

https://synbiohub.org/public/igem/BBa_J07014/annotation1582322/range1582322/1
type: Range
persistent identity: range1582322
display id: range1582322
version: 1
direction: direction/forward
top level: BBa_J07014
start: 61
end: 120
orientation: inline

CDR2 VH
type: SequenceAnnotation
persistent identity: annotation1582330
display id: annotation1582330
version: 1
top level: BBa_J07014
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J07014/annotation1582330/range1582330/1

https://synbiohub.org/public/igem/BBa_J07014/annotation1582330/range1582330/1
type: Range
persistent identity: range1582330
display id: range1582330
version: 1
direction: direction/forward
top level: BBa_J07014
start: 559
end: 615
orientation: inline

F-V
type: SequenceAnnotation
persistent identity: annotation1583271
display id: annotation1583271
version: 1
top level: BBa_J07014
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_J07014/annotation1583271/range1583271/1

https://synbiohub.org/public/igem/BBa_J07014/annotation1583271/range1583271/1
type: Range
persistent identity: range1583271
display id: range1583271
version: 1
direction: direction/forward
top level: BBa_J07014
start: 178
end: 180
orientation: inline

CDR3 VH
type: SequenceAnnotation
persistent identity: annotation1582331
display id: annotation1582331
version: 1
top level: BBa_J07014
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J07014/annotation1582331/range1582331/1

https://synbiohub.org/public/igem/BBa_J07014/annotation1582331/range1582331/1
type: Range
persistent identity: range1582331
display id: range1582331
version: 1
direction: direction/forward
top level: BBa_J07014
start: 711
end: 732
orientation: inline

S-N
type: SequenceAnnotation
persistent identity: annotation1583278
display id: annotation1583278
version: 1
top level: BBa_J07014
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_J07014/annotation1583278/range1583278/1

https://synbiohub.org/public/igem/BBa_J07014/annotation1583278/range1583278/1
type: Range
persistent identity: range1583278
display id: range1583278
version: 1
direction: direction/forward
top level: BBa_J07014
start: 241
end: 243
orientation: inline

S-A
type: SequenceAnnotation
persistent identity: annotation1582343
display id: annotation1582343
version: 1
top level: BBa_J07014
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_J07014/annotation1582343/range1582343/1

https://synbiohub.org/public/igem/BBa_J07014/annotation1582343/range1582343/1
type: Range
persistent identity: range1582343
display id: range1582343
version: 1
direction: direction/forward
top level: BBa_J07014
start: 712
end: 714
orientation: inline

I-F
type: SequenceAnnotation
persistent identity: annotation1583294
display id: annotation1583294
version: 1
top level: BBa_J07014
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_J07014/annotation1583294/range1583294/1

https://synbiohub.org/public/igem/BBa_J07014/annotation1583294/range1583294/1
type: Range
persistent identity: range1583294
display id: range1583294
version: 1
direction: direction/forward
top level: BBa_J07014
start: 562
end: 564
orientation: inline

S-G
type: SequenceAnnotation
persistent identity: annotation1583292
display id: annotation1583292
version: 1
top level: BBa_J07014
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_J07014/annotation1583292/range1583292/1

https://synbiohub.org/public/igem/BBa_J07014/annotation1583292/range1583292/1
type: Range
persistent identity: range1583292
display id: range1583292
version: 1
direction: direction/forward
top level: BBa_J07014
start: 499
end: 501
orientation: inline

R-G
type: SequenceAnnotation
persistent identity: annotation1583280
display id: annotation1583280
version: 1
top level: BBa_J07014
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_J07014/annotation1583280/range1583280/1

https://synbiohub.org/public/igem/BBa_J07014/annotation1583280/range1583280/1
type: Range
persistent identity: range1583280
display id: range1583280
version: 1
direction: direction/forward
top level: BBa_J07014
start: 457
end: 459
orientation: inline

VL
type: SequenceAnnotation
persistent identity: annotation1582335
display id: annotation1582335
version: 1
top level: BBa_J07014
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J07014/annotation1582335/range1582335/1

https://synbiohub.org/public/igem/BBa_J07014/annotation1582335/range1582335/1
type: Range
persistent identity: range1582335
display id: range1582335
version: 1
direction: direction/forward
top level: BBa_J07014
start: 1
end: 336
orientation: inline

D-E
type: SequenceAnnotation
persistent identity: annotation1583298
display id: annotation1583298
version: 1
top level: BBa_J07014
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_J07014/annotation1583298/range1583298/1

https://synbiohub.org/public/igem/BBa_J07014/annotation1583298/range1583298/1
type: Range
persistent identity: range1583298
display id: range1583298
version: 1
direction: direction/forward
top level: BBa_J07014
start: 727
end: 729
orientation: inline

D-H
type: SequenceAnnotation
persistent identity: annotation1583293
display id: annotation1583293
version: 1
top level: BBa_J07014
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_J07014/annotation1583293/range1583293/1

https://synbiohub.org/public/igem/BBa_J07014/annotation1583293/range1583293/1
type: Range
persistent identity: range1583293
display id: range1583293
version: 1
direction: direction/forward
top level: BBa_J07014
start: 502
end: 504
orientation: inline

A-T
type: SequenceAnnotation
persistent identity: annotation1583288
display id: annotation1583288
version: 1
top level: BBa_J07014
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_J07014/annotation1583288/range1583288/1

https://synbiohub.org/public/igem/BBa_J07014/annotation1583288/range1583288/1
type: Range
persistent identity: range1583288
display id: range1583288
version: 1
direction: direction/forward
top level: BBa_J07014
start: 481
end: 483
orientation: inline

W-L
type: SequenceAnnotation
persistent identity: annotation1583299
display id: annotation1583299
version: 1
top level: BBa_J07014
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_J07014/annotation1583299/range1583299/1

https://synbiohub.org/public/igem/BBa_J07014/annotation1583299/range1583299/1
type: Range
persistent identity: range1583299
display id: range1583299
version: 1
direction: direction/forward
top level: BBa_J07014
start: 733
end: 735
orientation: inline

Y-S
type: SequenceAnnotation
persistent identity: annotation1583297
display id: annotation1583297
version: 1
top level: BBa_J07014
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_J07014/annotation1583297/range1583297/1

https://synbiohub.org/public/igem/BBa_J07014/annotation1583297/range1583297/1
type: Range
persistent identity: range1583297
display id: range1583297
version: 1
direction: direction/forward
top level: BBa_J07014
start: 715
end: 717
orientation: inline

BBa_I722006
type: ComponentDefinition
persistent identity: BBa I722006
display id: BBa_I722006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: GFP Producer Controlled by 3OC6HSL (without Promoter)
creator: Zhu Manli, Cao Yingxiu, Wang Xing
created: 2007-10-19T11:00:00Z
modified: 2015-08-31T04:07:53Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _150_
m user id: 0
owner id: 1532
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: 3OC6HSL receiver device which need a PROMOTER to control the expression of LuxR
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Signalling
role: SO:0000804
component: BBa_E0840
component: BBa_A340620
sequence annotation: BBa_E0840
sequence annotation: BBa_A340620
sequence: https://synbiohub.org/public/igem/BBa_I722006_sequence/1

BBa_E0840
type: Component
persistent identity: component2275990
display id: component2275990
version: 1
top level: BBa_I722006
access: public
definition: https://synbiohub.org/public/igem/BBa_E0840/1

BBa_A340620
type: Component
persistent identity: component2275979
display id: component2275979
version: 1
top level: BBa_I722006
access: public
definition: https://synbiohub.org/public/igem/BBa_A340620/1

BBa_E0840
type: SequenceAnnotation
persistent identity: annotation2275990
display id: annotation2275990
version: 1
top level: BBa_I722006
location: https://synbiohub.org/public/igem/BBa_I722006/annotation2275990/range2275990/1
component: BBa_E0840

https://synbiohub.org/public/igem/BBa_I722006/annotation2275990/range2275990/1
type: Range
persistent identity: range2275990
display id: range2275990
version: 1
direction: direction/reverse
top level: BBa_I722006
start: 1008
end: 1885
orientation: inline

BBa_A340620
type: SequenceAnnotation
persistent identity: annotation2275979
display id: annotation2275979
version: 1
top level: BBa_I722006
location: https://synbiohub.org/public/igem/BBa_I722006/annotation2275979/range2275979/1
component: BBa_A340620

https://synbiohub.org/public/igem/BBa_I722006/annotation2275979/range2275979/1
type: Range
persistent identity: range2275979
display id: range2275979
version: 1
direction: direction/reverse
top level: BBa_I722006
start: 1
end: 999
orientation: inline

BBa_K1520508
type: ComponentDefinition
persistent identity: BBa K1520508
display id: BBa_K1520508
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PgolTS-golS-PgolB-rbs-luxI-Ter
creator: yue wu
created: 2014-09-22T11:00:00Z
modified: 2015-05-08T01:10:49Z
discontinued: false
dominant: false
group u list: _1902_
m user id: 0
owner id: 21318
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: PgolTS-golS-PgolB-rbs-luxI-Ter If there are gold ions, the luxI will be produced.
mutable notes: None
mutable provenance: PgolTS-golS-PgolB, luxI, Ter
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1520506
component: BBa_K1520029
component: BBa_K1520504
sequence annotation: BBa_K1520506
sequence annotation: BBa_K1520504
sequence annotation: BBa_K1520029
sequence: https://synbiohub.org/public/igem/BBa_K1520508_sequence/1

BBa_K1520506
type: Component
persistent identity: component2385519
display id: component2385519
version: 1
top level: BBa_K1520508
access: public
definition: https://synbiohub.org/public/igem/BBa_K1520506/1

BBa_K1520029
type: Component
persistent identity: component2385523
display id: component2385523
version: 1
top level: BBa_K1520508
access: public
definition: https://synbiohub.org/public/igem/BBa_K1520029/1

BBa_K1520504
type: Component
persistent identity: component2385522
display id: component2385522
version: 1
top level: BBa_K1520508
access: public
definition: BBa_K1520504

BBa_K1520506
type: SequenceAnnotation
persistent identity: annotation2385519
display id: annotation2385519
version: 1
top level: BBa_K1520508
location: https://synbiohub.org/public/igem/BBa_K1520508/annotation2385519/range2385519/1
component: BBa_K1520506

https://synbiohub.org/public/igem/BBa_K1520508/annotation2385519/range2385519/1
type: Range
persistent identity: range2385519
display id: range2385519
version: 1
direction: direction/reverse
top level: BBa_K1520508
start: 1
end: 753
orientation: inline

BBa_K1520504
type: SequenceAnnotation
persistent identity: annotation2385522
display id: annotation2385522
version: 1
top level: BBa_K1520508
location: https://synbiohub.org/public/igem/BBa_K1520508/annotation2385522/range2385522/1
component: BBa_K1520504

https://synbiohub.org/public/igem/BBa_K1520508/annotation2385522/range2385522/1
type: Range
persistent identity: range2385522
display id: range2385522
version: 1
direction: direction/reverse
top level: BBa_K1520508
start: 762
end: 1422
orientation: inline

BBa_K1520029
type: SequenceAnnotation
persistent identity: annotation2385523
display id: annotation2385523
version: 1
top level: BBa_K1520508
location: https://synbiohub.org/public/igem/BBa_K1520508/annotation2385523/range2385523/1
component: BBa_K1520029

https://synbiohub.org/public/igem/BBa_K1520508/annotation2385523/range2385523/1
type: Range
persistent identity: range2385523
display id: range2385523
version: 1
direction: direction/reverse
top level: BBa_K1520508
start: 1431
end: 1559
orientation: inline

LuxR
type: ComponentDefinition
persistent identity: BBa I0462
display id: BBa_I0462
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: luxR Protein Generator
creator: Caitlin Conboy and Jennifer Braff
created: 2003-12-04T12:00:00Z
modified: 2015-08-31T04:07:29Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Produces LuxR protein which can sense 3OC6HSL in the media and activate transcription from R0062, the right hand Lux promoter
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0012
component: BBa_B0034
component: BBa_C0062
component: BBa_B0010
sequence annotation: BBa_C0062
sequence annotation: BBa_B0012
sequence annotation: BBa_B0034
sequence annotation: BBa_B0010
sequence: https://synbiohub.org/public/igem/BBa_I0462_sequence/1

BBa_B0012
type: Component
persistent identity: component943199
display id: component943199
version: 1
top level: LuxR
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0034
type: Component
persistent identity: component943166
display id: component943166
version: 1
top level: LuxR
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_C0062
type: Component
persistent identity: component943181
display id: component943181
version: 1
top level: LuxR
access: public
definition: luxr

BBa_B0010
type: Component
persistent identity: component943189
display id: component943189
version: 1
top level: LuxR
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_C0062
type: SequenceAnnotation
persistent identity: annotation943181
display id: annotation943181
version: 1
top level: LuxR
location: https://synbiohub.org/public/igem/BBa_I0462/annotation943181/range943181/1
component: BBa_C0062

https://synbiohub.org/public/igem/BBa_I0462/annotation943181/range943181/1
type: Range
persistent identity: range943181
display id: range943181
version: 1
direction: direction/forward
top level: LuxR
start: 19
end: 774
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation943199
display id: annotation943199
version: 1
top level: LuxR
location: https://synbiohub.org/public/igem/BBa_I0462/annotation943199/range943199/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I0462/annotation943199/range943199/1
type: Range
persistent identity: range943199
display id: range943199
version: 1
direction: direction/forward
top level: LuxR
start: 896
end: 936
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation943166
display id: annotation943166
version: 1
top level: LuxR
location: https://synbiohub.org/public/igem/BBa_I0462/annotation943166/range943166/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I0462/annotation943166/range943166/1
type: Range
persistent identity: range943166
display id: range943166
version: 1
direction: direction/forward
top level: LuxR
start: 1
end: 12
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation943189
display id: annotation943189
version: 1
top level: LuxR
location: https://synbiohub.org/public/igem/BBa_I0462/annotation943189/range943189/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I0462/annotation943189/range943189/1
type: Range
persistent identity: range943189
display id: range943189
version: 1
direction: direction/forward
top level: LuxR
start: 808
end: 887
orientation: inline

BBa_I13273
type: ComponentDefinition
persistent identity: BBa I13273
display id: BBa_I13273
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: YFP Producer Controlled by 3OC6HSL Receiver Device
creator: Barry Canton
created: 2004-08-08T11:00:00Z
modified: 2015-08-31T04:07:33Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _101_6_
m user id: 0
owner id: 135
owning group id: 7
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: The luxR based receiver, I13270, controls the production of the YFP. This construct has been used to characterize I13270.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Signalling
role: SO:0000804
component: BBa_E0030
component: BBa_B0010
component: BBa_B0034
component: BBa_B0012
component: BBa_R0040
component: BBa_R0062
component: BBa_C0062
sequence annotation: BBa_R0040
sequence annotation: BBa_B0012
sequence annotation: BBa_B0010
sequence annotation: BBa_C0062
sequence annotation: BBa_E0030
sequence annotation: BBa_R0062
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_I13273_sequence/1

BBa_E0030
type: Component
persistent identity: component1506584
display id: component1506584
version: 1
top level: BBa_I13273
access: public
definition: https://synbiohub.org/public/igem/BBa_E0030/1

BBa_B0010
type: Component
persistent identity: component1506549
display id: component1506549
version: 1
top level: BBa_I13273
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0034
type: Component
persistent identity: component1506526
display id: component1506526
version: 1
top level: BBa_I13273
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0012
type: Component
persistent identity: component1506599
display id: component1506599
version: 1
top level: BBa_I13273
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_R0040
type: Component
persistent identity: component1506518
display id: component1506518
version: 1
top level: BBa_I13273
access: public
definition: https://synbiohub.org/public/igem/BBa_R0040/1

BBa_R0062
type: Component
persistent identity: component1506574
display id: component1506574
version: 1
top level: BBa_I13273
access: public
definition: https://synbiohub.org/public/igem/BBa_R0062/1

BBa_B0034
type: Component
persistent identity: component1506582
display id: component1506582
version: 1
top level: BBa_I13273
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0010
type: Component
persistent identity: component1506589
display id: component1506589
version: 1
top level: BBa_I13273
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
type: Component
persistent identity: component1506559
display id: component1506559
version: 1
top level: BBa_I13273
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_C0062
type: Component
persistent identity: component1506541
display id: component1506541
version: 1
top level: BBa_I13273
access: public
definition: luxr

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1506518
display id: annotation1506518
version: 1
top level: BBa_I13273
location: https://synbiohub.org/public/igem/BBa_I13273/annotation1506518/range1506518/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_I13273/annotation1506518/range1506518/1
type: Range
persistent identity: range1506518
display id: range1506518
version: 1
direction: direction/forward
top level: BBa_I13273
start: 1
end: 54
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1506559
display id: annotation1506559
version: 1
top level: BBa_I13273
location: https://synbiohub.org/public/igem/BBa_I13273/annotation1506559/range1506559/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I13273/annotation1506559/range1506559/1
type: Range
persistent identity: range1506559
display id: range1506559
version: 1
direction: direction/forward
top level: BBa_I13273
start: 958
end: 998
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1506549
display id: annotation1506549
version: 1
top level: BBa_I13273
location: https://synbiohub.org/public/igem/BBa_I13273/annotation1506549/range1506549/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I13273/annotation1506549/range1506549/1
type: Range
persistent identity: range1506549
display id: range1506549
version: 1
direction: direction/forward
top level: BBa_I13273
start: 870
end: 949
orientation: inline

BBa_C0062
type: SequenceAnnotation
persistent identity: annotation1506541
display id: annotation1506541
version: 1
top level: BBa_I13273
location: https://synbiohub.org/public/igem/BBa_I13273/annotation1506541/range1506541/1
component: BBa_C0062

https://synbiohub.org/public/igem/BBa_I13273/annotation1506541/range1506541/1
type: Range
persistent identity: range1506541
display id: range1506541
version: 1
direction: direction/forward
top level: BBa_I13273
start: 81
end: 836
orientation: inline

BBa_E0030
type: SequenceAnnotation
persistent identity: annotation1506584
display id: annotation1506584
version: 1
top level: BBa_I13273
location: https://synbiohub.org/public/igem/BBa_I13273/annotation1506584/range1506584/1
component: BBa_E0030

https://synbiohub.org/public/igem/BBa_I13273/annotation1506584/range1506584/1
type: Range
persistent identity: range1506584
display id: range1506584
version: 1
direction: direction/forward
top level: BBa_I13273
start: 1088
end: 1810
orientation: inline

BBa_R0062
type: SequenceAnnotation
persistent identity: annotation1506574
display id: annotation1506574
version: 1
top level: BBa_I13273
location: https://synbiohub.org/public/igem/BBa_I13273/annotation1506574/range1506574/1
component: BBa_R0062

https://synbiohub.org/public/igem/BBa_I13273/annotation1506574/range1506574/1
type: Range
persistent identity: range1506574
display id: range1506574
version: 1
direction: direction/forward
top level: BBa_I13273
start: 1007
end: 1061
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1506589
display id: annotation1506589
version: 1
top level: BBa_I13273
location: https://synbiohub.org/public/igem/BBa_I13273/annotation1506589/range1506589/1
component: BBa_B0010

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type: Range
persistent identity: range1506589
display id: range1506589
version: 1
direction: direction/forward
top level: BBa_I13273
start: 1819
end: 1898
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1506526
display id: annotation1506526
version: 1
top level: BBa_I13273
location: https://synbiohub.org/public/igem/BBa_I13273/annotation1506526/range1506526/1
component: BBa_B0034

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type: Range
persistent identity: range1506526
display id: range1506526
version: 1
direction: direction/forward
top level: BBa_I13273
start: 63
end: 74
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1506582
display id: annotation1506582
version: 1
top level: BBa_I13273
location: https://synbiohub.org/public/igem/BBa_I13273/annotation1506582/range1506582/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I13273/annotation1506582/range1506582/1
type: Range
persistent identity: range1506582
display id: range1506582
version: 1
direction: direction/forward
top level: BBa_I13273
start: 1070
end: 1081
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1506599
display id: annotation1506599
version: 1
top level: BBa_I13273
location: https://synbiohub.org/public/igem/BBa_I13273/annotation1506599/range1506599/1
component: BBa_B0012

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type: Range
persistent identity: range1506599
display id: range1506599
version: 1
direction: direction/forward
top level: BBa_I13273
start: 1907
end: 1947
orientation: inline

BBa_K091190
type: ComponentDefinition
persistent identity: BBa K091190
display id: BBa_K091190
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: plux/cI+RBS+lasI+RBS+Mnt+TT+pMnt/lac+RBS+lasI+RBS+cI+TT
creator: Xiao Zhu, Alicia Allen, Andrew Gordon
created: 2008-08-07T11:00:00Z
modified: 2015-05-08T01:08:39Z
discontinued: false
dominant: false
experience: experience/None
group u list: _191_
m user id: 0
owner id: 3130
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: send P-AI-1 signaling molecules out, using Mnt(or cI) protein repress the pMnt/lac( or plux/cI) hybrid promoter
mutable notes: .
mutable provenance: .
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_K091162
component: BBa_K091188
sequence annotation: BBa_K091162
sequence annotation: BBa_K091188
sequence: https://synbiohub.org/public/igem/BBa_K091190_sequence/1

BBa_K091162
type: Component
persistent identity: component2302013
display id: component2302013
version: 1
top level: BBa_K091190
access: public
definition: https://synbiohub.org/public/igem/BBa_K091162/1

BBa_K091188
type: Component
persistent identity: component2302037
display id: component2302037
version: 1
top level: BBa_K091190
access: public
definition: https://synbiohub.org/public/igem/BBa_K091188/1

BBa_K091162
type: SequenceAnnotation
persistent identity: annotation2302013
display id: annotation2302013
version: 1
top level: BBa_K091190
location: https://synbiohub.org/public/igem/BBa_K091190/annotation2302013/range2302013/1
component: BBa_K091162

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type: Range
persistent identity: range2302013
display id: range2302013
version: 1
direction: direction/reverse
top level: BBa_K091190
start: 1
end: 1168
orientation: inline

BBa_K091188
type: SequenceAnnotation
persistent identity: annotation2302037
display id: annotation2302037
version: 1
top level: BBa_K091190
location: https://synbiohub.org/public/igem/BBa_K091190/annotation2302037/range2302037/1
component: BBa_K091188

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type: Range
persistent identity: range2302037
display id: range2302037
version: 1
direction: direction/reverse
top level: BBa_K091190
start: 1177
end: 2802
orientation: inline

BBa_I726031
type: ComponentDefinition
persistent identity: BBa I726031
display id: BBa_I726031
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Trc-LIC
creator: Bangalore iGEM
created: 2007-07-03T11:00:00Z
modified: 2015-08-31T04:07:55Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _107_
m user id: 0
owner id: 2077
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Abcdefghijkl..
mutable notes: none
mutable provenance: Registry
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_E0020
component: BBa_B0010
component: BBa_B0034
component: BBa_R0011
component: BBa_B0012
component: BBa_C0161
sequence annotation: BBa_R0011
sequence annotation: BBa_E0020
sequence annotation: BBa_B0034
sequence annotation: BBa_C0161
sequence annotation: BBa_B0012
sequence annotation: BBa_B0010
sequence: https://synbiohub.org/public/igem/BBa_I726031_sequence/1

BBa_E0020
type: Component
persistent identity: component1951367
display id: component1951367
version: 1
top level: BBa_I726031
access: public
definition: https://synbiohub.org/public/igem/BBa_E0020/1

BBa_B0010
type: Component
persistent identity: component1951368
display id: component1951368
version: 1
top level: BBa_I726031
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0034
type: Component
persistent identity: component1951362
display id: component1951362
version: 1
top level: BBa_I726031
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_R0011
type: Component
persistent identity: component1951356
display id: component1951356
version: 1
top level: BBa_I726031
access: public
definition: https://synbiohub.org/public/igem/BBa_R0011/1

BBa_B0034
type: Component
persistent identity: component1951366
display id: component1951366
version: 1
top level: BBa_I726031
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0012
type: Component
persistent identity: component1951370
display id: component1951370
version: 1
top level: BBa_I726031
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_C0161
type: Component
persistent identity: component1951364
display id: component1951364
version: 1
top level: BBa_I726031
access: public
definition: luxI

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation1951356
display id: annotation1951356
version: 1
top level: BBa_I726031
location: https://synbiohub.org/public/igem/BBa_I726031/annotation1951356/range1951356/1
component: BBa_R0011

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type: Range
persistent identity: range1951356
display id: range1951356
version: 1
direction: direction/forward
top level: BBa_I726031
start: 1
end: 54
orientation: inline

BBa_E0020
type: SequenceAnnotation
persistent identity: annotation1951367
display id: annotation1951367
version: 1
top level: BBa_I726031
location: https://synbiohub.org/public/igem/BBa_I726031/annotation1951367/range1951367/1
component: BBa_E0020

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type: Range
persistent identity: range1951367
display id: range1951367
version: 1
direction: direction/reverse
top level: BBa_I726031
start: 693
end: 1415
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1951362
display id: annotation1951362
version: 1
top level: BBa_I726031
location: https://synbiohub.org/public/igem/BBa_I726031/annotation1951362/range1951362/1
component: BBa_B0034

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type: Range
persistent identity: range1951362
display id: range1951362
version: 1
direction: direction/reverse
top level: BBa_I726031
start: 64
end: 75
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1951366
display id: annotation1951366
version: 1
top level: BBa_I726031
location: https://synbiohub.org/public/igem/BBa_I726031/annotation1951366/range1951366/1
component: BBa_B0034

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type: Range
persistent identity: range1951366
display id: range1951366
version: 1
direction: direction/reverse
top level: BBa_I726031
start: 675
end: 686
orientation: inline

BBa_C0161
type: SequenceAnnotation
persistent identity: annotation1951364
display id: annotation1951364
version: 1
top level: BBa_I726031
location: https://synbiohub.org/public/igem/BBa_I726031/annotation1951364/range1951364/1
component: BBa_C0161

https://synbiohub.org/public/igem/BBa_I726031/annotation1951364/range1951364/1
type: Range
persistent identity: range1951364
display id: range1951364
version: 1
direction: direction/reverse
top level: BBa_I726031
start: 82
end: 666
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1951370
display id: annotation1951370
version: 1
top level: BBa_I726031
location: https://synbiohub.org/public/igem/BBa_I726031/annotation1951370/range1951370/1
component: BBa_B0012

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type: Range
persistent identity: range1951370
display id: range1951370
version: 1
direction: direction/forward
top level: BBa_I726031
start: 1512
end: 1552
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1951368
display id: annotation1951368
version: 1
top level: BBa_I726031
location: https://synbiohub.org/public/igem/BBa_I726031/annotation1951368/range1951368/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I726031/annotation1951368/range1951368/1
type: Range
persistent identity: range1951368
display id: range1951368
version: 1
direction: direction/forward
top level: BBa_I726031
start: 1424
end: 1503
orientation: inline

BBa_I13274
type: ComponentDefinition
persistent identity: BBa I13274
display id: BBa_I13274
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Lux Receiver (HSL & R0011 driven)
creator: Barry Canton
created: 2004-08-25T11:00:00Z
modified: 2015-08-31T04:07:33Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_6_
m user id: 0
owner id: 135
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Receiver circuit. LuxR production is controlled by R0011. Output device is untagged YFP. Because R0011 is stronger than R0040, the switch point of this receiver should be lower than I13273. Built from the following parts - R0011.A340620.E0430
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Signalling
role: SO:0000804
component: BBa_R0011
component: BBa_B0034
component: BBa_E0430
component: BBa_C0062
component: BBa_B0015
component: BBa_R0062
sequence annotation: BBa_B0015
sequence annotation: BBa_B0034
sequence annotation: BBa_R0062
sequence annotation: BBa_R0011
sequence annotation: BBa_E0430
sequence annotation: BBa_C0062
sequence: https://synbiohub.org/public/igem/BBa_I13274_sequence/1

BBa_R0011
type: Component
persistent identity: component2229786
display id: component2229786
version: 1
top level: BBa_I13274
access: public
definition: https://synbiohub.org/public/igem/BBa_R0011/1

BBa_B0034
type: Component
persistent identity: component2229792
display id: component2229792
version: 1
top level: BBa_I13274
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_E0430
type: Component
persistent identity: component2229820
display id: component2229820
version: 1
top level: BBa_I13274
access: public
definition: https://synbiohub.org/public/igem/BBa_E0430/1

BBa_C0062
type: Component
persistent identity: component2229795
display id: component2229795
version: 1
top level: BBa_I13274
access: public
definition: luxr

BBa_B0015
type: Component
persistent identity: component2229805
display id: component2229805
version: 1
top level: BBa_I13274
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_R0062
type: Component
persistent identity: component2229807
display id: component2229807
version: 1
top level: BBa_I13274
access: public
definition: https://synbiohub.org/public/igem/BBa_R0062/1

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2229805
display id: annotation2229805
version: 1
top level: BBa_I13274
location: https://synbiohub.org/public/igem/BBa_I13274/annotation2229805/range2229805/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_I13274/annotation2229805/range2229805/1
type: Range
persistent identity: range2229805
display id: range2229805
version: 1
direction: direction/reverse
top level: BBa_I13274
start: 871
end: 999
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2229792
display id: annotation2229792
version: 1
top level: BBa_I13274
location: https://synbiohub.org/public/igem/BBa_I13274/annotation2229792/range2229792/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I13274/annotation2229792/range2229792/1
type: Range
persistent identity: range2229792
display id: range2229792
version: 1
direction: direction/reverse
top level: BBa_I13274
start: 64
end: 75
orientation: inline

BBa_R0062
type: SequenceAnnotation
persistent identity: annotation2229807
display id: annotation2229807
version: 1
top level: BBa_I13274
location: https://synbiohub.org/public/igem/BBa_I13274/annotation2229807/range2229807/1
component: BBa_R0062

https://synbiohub.org/public/igem/BBa_I13274/annotation2229807/range2229807/1
type: Range
persistent identity: range2229807
display id: range2229807
version: 1
direction: direction/forward
top level: BBa_I13274
start: 1008
end: 1062
orientation: inline

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation2229786
display id: annotation2229786
version: 1
top level: BBa_I13274
location: https://synbiohub.org/public/igem/BBa_I13274/annotation2229786/range2229786/1
component: BBa_R0011

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type: Range
persistent identity: range2229786
display id: range2229786
version: 1
direction: direction/forward
top level: BBa_I13274
start: 1
end: 54
orientation: inline

BBa_E0430
type: SequenceAnnotation
persistent identity: annotation2229820
display id: annotation2229820
version: 1
top level: BBa_I13274
location: https://synbiohub.org/public/igem/BBa_I13274/annotation2229820/range2229820/1
component: BBa_E0430

https://synbiohub.org/public/igem/BBa_I13274/annotation2229820/range2229820/1
type: Range
persistent identity: range2229820
display id: range2229820
version: 1
direction: direction/reverse
top level: BBa_I13274
start: 1071
end: 1948
orientation: inline

BBa_C0062
type: SequenceAnnotation
persistent identity: annotation2229795
display id: annotation2229795
version: 1
top level: BBa_I13274
location: https://synbiohub.org/public/igem/BBa_I13274/annotation2229795/range2229795/1
component: BBa_C0062

https://synbiohub.org/public/igem/BBa_I13274/annotation2229795/range2229795/1
type: Range
persistent identity: range2229795
display id: range2229795
version: 1
direction: direction/forward
top level: BBa_I13274
start: 82
end: 837
orientation: inline

BBa_K211002
type: ComponentDefinition
persistent identity: BBa K211002
display id: BBa_K211002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RI7-odr10 chimeric GPCR
creator: ZHONG Wanting
created: 2009-10-12T11:00:00Z
modified: 2015-05-08T01:11:29Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _313_
m user id: 0
owner id: 4277
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: The chimeric GPCR consists of the N- and C- terminals of the rat OR RI7, flanking the TM2-TM7 ligand-binding domain of the c. elegans OR odr-10. It can be functionally expressed in S. cerevisiae, activating the yeast endogenous MAPK pathway upon binding to the ligand diacetyl.
mutable provenance: C. elegans, Rattus norvegicus
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: stop
sequence annotation: membrane localization sequence
sequence annotation: start
sequence annotation: diacetyl sensing region
sequence annotation: Gasubunit coupling sequence
sequence: https://synbiohub.org/public/igem/BBa_K211002_sequence/1

stop
type: SequenceAnnotation
persistent identity: annotation2035762
display id: annotation2035762
version: 1
top level: BBa_K211002
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K211002/annotation2035762/range2035762/1

https://synbiohub.org/public/igem/BBa_K211002/annotation2035762/range2035762/1
type: Range
persistent identity: range2035762
display id: range2035762
version: 1
direction: direction/forward
top level: BBa_K211002
start: 1057
end: 1062
orientation: inline

membrane localization sequence
type: SequenceAnnotation
persistent identity: annotation2049258
display id: annotation2049258
version: 1
top level: BBa_K211002
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K211002/annotation2049258/range2049258/1

https://synbiohub.org/public/igem/BBa_K211002/annotation2049258/range2049258/1
type: Range
persistent identity: range2049258
display id: range2049258
version: 1
direction: direction/forward
top level: BBa_K211002
start: 1
end: 183
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2035761
display id: annotation2035761
version: 1
top level: BBa_K211002
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K211002/annotation2035761/range2035761/1

https://synbiohub.org/public/igem/BBa_K211002/annotation2035761/range2035761/1
type: Range
persistent identity: range2035761
display id: range2035761
version: 1
direction: direction/forward
top level: BBa_K211002
start: 1
end: 3
orientation: inline

diacetyl sensing region
type: SequenceAnnotation
persistent identity: annotation2049260
display id: annotation2049260
version: 1
top level: BBa_K211002
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K211002/annotation2049260/range2049260/1

https://synbiohub.org/public/igem/BBa_K211002/annotation2049260/range2049260/1
type: Range
persistent identity: range2049260
display id: range2049260
version: 1
direction: direction/forward
top level: BBa_K211002
start: 184
end: 957
orientation: inline

Gasubunit coupling sequence
type: SequenceAnnotation
persistent identity: annotation2049259
display id: annotation2049259
version: 1
top level: BBa_K211002
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K211002/annotation2049259/range2049259/1

https://synbiohub.org/public/igem/BBa_K211002/annotation2049259/range2049259/1
type: Range
persistent identity: range2049259
display id: range2049259
version: 1
direction: direction/forward
top level: BBa_K211002
start: 958
end: 1062
orientation: inline

BBa_J06400
type: ComponentDefinition
persistent identity: BBa J06400
display id: BBa_J06400
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: 3OC12HSL Receiver
creator: kxjin
created: 2005-06-21T11:00:00Z
modified: 2015-08-31T04:08:17Z
discontinued: false
dominant: false
experience: experience/None
group u list: _20_
m user id: 0
owner id: 368
owning group id: 20
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This device senses the 3OC12HSL concentration in the media and produces output PoPS. LasR production is controlled by constitutive P(BIa). This part is the 3OC12HSL analog of F2621.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Signalling
role: SO:0000804
component: BBa_B0010
component: BBa_B0012
component: BBa_I14033
component: BBa_B0034
component: BBa_C0079
component: BBa_R0079
sequence annotation: BBa_R0079
sequence annotation: BBa_B0010
sequence annotation: BBa_B0034
sequence annotation: BBa_C0079
sequence annotation: BBa_B0012
sequence annotation: BBa_I14033
sequence: https://synbiohub.org/public/igem/BBa_J06400_sequence/1

BBa_B0010
type: Component
persistent identity: component1672452
display id: component1672452
version: 1
top level: BBa_J06400
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
type: Component
persistent identity: component1672462
display id: component1672462
version: 1
top level: BBa_J06400
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_I14033
type: Component
persistent identity: component1672428
display id: component1672428
version: 1
top level: BBa_J06400
access: public
definition: https://synbiohub.org/public/igem/BBa_I14033/1

BBa_B0034
type: Component
persistent identity: component1672433
display id: component1672433
version: 1
top level: BBa_J06400
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_C0079
type: Component
persistent identity: component1672446
display id: component1672446
version: 1
top level: BBa_J06400
access: public
definition: lasr

BBa_R0079
type: Component
persistent identity: component1672479
display id: component1672479
version: 1
top level: BBa_J06400
access: public
definition: https://synbiohub.org/public/igem/BBa_R0079/1

BBa_R0079
type: SequenceAnnotation
persistent identity: annotation1672479
display id: annotation1672479
version: 1
top level: BBa_J06400
location: https://synbiohub.org/public/igem/BBa_J06400/annotation1672479/range1672479/1
component: BBa_R0079

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persistent identity: range1672479
display id: range1672479
version: 1
direction: direction/forward
top level: BBa_J06400
start: 991
end: 1147
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1672452
display id: annotation1672452
version: 1
top level: BBa_J06400
location: https://synbiohub.org/public/igem/BBa_J06400/annotation1672452/range1672452/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_J06400/annotation1672452/range1672452/1
type: Range
persistent identity: range1672452
display id: range1672452
version: 1
direction: direction/forward
top level: BBa_J06400
start: 854
end: 933
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1672433
display id: annotation1672433
version: 1
top level: BBa_J06400
location: https://synbiohub.org/public/igem/BBa_J06400/annotation1672433/range1672433/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_J06400/annotation1672433/range1672433/1
type: Range
persistent identity: range1672433
display id: range1672433
version: 1
direction: direction/forward
top level: BBa_J06400
start: 47
end: 58
orientation: inline

BBa_C0079
type: SequenceAnnotation
persistent identity: annotation1672446
display id: annotation1672446
version: 1
top level: BBa_J06400
location: https://synbiohub.org/public/igem/BBa_J06400/annotation1672446/range1672446/1
component: BBa_C0079

https://synbiohub.org/public/igem/BBa_J06400/annotation1672446/range1672446/1
type: Range
persistent identity: range1672446
display id: range1672446
version: 1
direction: direction/forward
top level: BBa_J06400
start: 65
end: 820
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1672462
display id: annotation1672462
version: 1
top level: BBa_J06400
location: https://synbiohub.org/public/igem/BBa_J06400/annotation1672462/range1672462/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_J06400/annotation1672462/range1672462/1
type: Range
persistent identity: range1672462
display id: range1672462
version: 1
direction: direction/forward
top level: BBa_J06400
start: 942
end: 982
orientation: inline

BBa_I14033
type: SequenceAnnotation
persistent identity: annotation1672428
display id: annotation1672428
version: 1
top level: BBa_J06400
location: https://synbiohub.org/public/igem/BBa_J06400/annotation1672428/range1672428/1
component: BBa_I14033

https://synbiohub.org/public/igem/BBa_J06400/annotation1672428/range1672428/1
type: Range
persistent identity: range1672428
display id: range1672428
version: 1
direction: direction/forward
top level: BBa_J06400
start: 1
end: 38
orientation: inline

Sen
type: ComponentDefinition
persistent identity: BBa I726041
display id: BBa_I726041
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Sen-TIC
creator: Bangalore iGEM
created: 2007-07-03T11:00:00Z
modified: 2015-08-31T04:07:55Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _107_
m user id: 0
owner id: 2077
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: Abcdefghijkl..
mutable notes: none
mutable provenance: Registry
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_B0034
component: BBa_B0012
component: BBa_B0010
component: BBa_R0040
component: BBa_E0020
component: BBa_C0161
sequence annotation: BBa_B0034
sequence annotation: BBa_B0012
sequence annotation: BBa_C0161
sequence annotation: BBa_B0010
sequence annotation: BBa_E0020
sequence annotation: BBa_R0040
sequence: https://synbiohub.org/public/igem/BBa_I726041_sequence/1

BBa_B0034
type: Component
persistent identity: component1951344
display id: component1951344
version: 1
top level: Sen
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0012
type: Component
persistent identity: component1951352
display id: component1951352
version: 1
top level: Sen
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0034
type: Component
persistent identity: component1951348
display id: component1951348
version: 1
top level: Sen
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0010
type: Component
persistent identity: component1951350
display id: component1951350
version: 1
top level: Sen
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_R0040
type: Component
persistent identity: component1951339
display id: component1951339
version: 1
top level: Sen
access: public
definition: https://synbiohub.org/public/igem/BBa_R0040/1

BBa_E0020
type: Component
persistent identity: component1951349
display id: component1951349
version: 1
top level: Sen
access: public
definition: https://synbiohub.org/public/igem/BBa_E0020/1

BBa_C0161
type: Component
persistent identity: component1951346
display id: component1951346
version: 1
top level: Sen
access: public
definition: luxI

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1951348
display id: annotation1951348
version: 1
top level: Sen
location: https://synbiohub.org/public/igem/BBa_I726041/annotation1951348/range1951348/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I726041/annotation1951348/range1951348/1
type: Range
persistent identity: range1951348
display id: range1951348
version: 1
direction: direction/reverse
top level: Sen
start: 674
end: 685
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1951352
display id: annotation1951352
version: 1
top level: Sen
location: https://synbiohub.org/public/igem/BBa_I726041/annotation1951352/range1951352/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I726041/annotation1951352/range1951352/1
type: Range
persistent identity: range1951352
display id: range1951352
version: 1
direction: direction/forward
top level: Sen
start: 1511
end: 1551
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1951344
display id: annotation1951344
version: 1
top level: Sen
location: https://synbiohub.org/public/igem/BBa_I726041/annotation1951344/range1951344/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I726041/annotation1951344/range1951344/1
type: Range
persistent identity: range1951344
display id: range1951344
version: 1
direction: direction/reverse
top level: Sen
start: 63
end: 74
orientation: inline

BBa_C0161
type: SequenceAnnotation
persistent identity: annotation1951346
display id: annotation1951346
version: 1
top level: Sen
location: https://synbiohub.org/public/igem/BBa_I726041/annotation1951346/range1951346/1
component: BBa_C0161

https://synbiohub.org/public/igem/BBa_I726041/annotation1951346/range1951346/1
type: Range
persistent identity: range1951346
display id: range1951346
version: 1
direction: direction/reverse
top level: Sen
start: 81
end: 665
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1951350
display id: annotation1951350
version: 1
top level: Sen
location: https://synbiohub.org/public/igem/BBa_I726041/annotation1951350/range1951350/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I726041/annotation1951350/range1951350/1
type: Range
persistent identity: range1951350
display id: range1951350
version: 1
direction: direction/forward
top level: Sen
start: 1423
end: 1502
orientation: inline

BBa_E0020
type: SequenceAnnotation
persistent identity: annotation1951349
display id: annotation1951349
version: 1
top level: Sen
location: https://synbiohub.org/public/igem/BBa_I726041/annotation1951349/range1951349/1
component: BBa_E0020

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type: Range
persistent identity: range1951349
display id: range1951349
version: 1
direction: direction/reverse
top level: Sen
start: 692
end: 1414
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1951339
display id: annotation1951339
version: 1
top level: Sen
location: https://synbiohub.org/public/igem/BBa_I726041/annotation1951339/range1951339/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_I726041/annotation1951339/range1951339/1
type: Range
persistent identity: range1951339
display id: range1951339
version: 1
direction: direction/forward
top level: Sen
start: 1
end: 54
orientation: inline

Stalker
type: ComponentDefinition
persistent identity: BBa J06001
display id: BBa_J06001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Tet repressible luxI generator ( LVA+ )
creator: sash31
created: 2005-06-12T11:00:00Z
modified: 2015-08-31T04:08:16Z
discontinued: false
dominant: true
experience: experience/None
group u list: _20_
m user id: 0
owner id: 339
owning group id: 20
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: A part designed to emit AHL when given aTc. Uses R0040 and F1610.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Signalling
role: SO:0000804
component: BBa_B0012
component: BBa_R0040
component: BBa_C0061
component: BBa_B0034
component: BBa_B0010
sequence annotation: BBa_B0034
sequence annotation: BBa_B0010
sequence annotation: BBa_R0040
sequence annotation: BBa_C0061
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_J06001_sequence/1

BBa_B0012
type: Component
persistent identity: component1512873
display id: component1512873
version: 1
top level: Stalker
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_R0040
type: Component
persistent identity: component1512838
display id: component1512838
version: 1
top level: Stalker
access: public
definition: https://synbiohub.org/public/igem/BBa_R0040/1

BBa_C0061
type: Component
persistent identity: component1512856
display id: component1512856
version: 1
top level: Stalker
access: public
definition: luxI

BBa_B0034
type: Component
persistent identity: component1512846
display id: component1512846
version: 1
top level: Stalker
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0010
type: Component
persistent identity: component1512863
display id: component1512863
version: 1
top level: Stalker
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1512846
display id: annotation1512846
version: 1
top level: Stalker
location: https://synbiohub.org/public/igem/BBa_J06001/annotation1512846/range1512846/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_J06001/annotation1512846/range1512846/1
type: Range
persistent identity: range1512846
display id: range1512846
version: 1
direction: direction/forward
top level: Stalker
start: 63
end: 74
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1512863
display id: annotation1512863
version: 1
top level: Stalker
location: https://synbiohub.org/public/igem/BBa_J06001/annotation1512863/range1512863/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_J06001/annotation1512863/range1512863/1
type: Range
persistent identity: range1512863
display id: range1512863
version: 1
direction: direction/forward
top level: Stalker
start: 732
end: 811
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1512838
display id: annotation1512838
version: 1
top level: Stalker
location: https://synbiohub.org/public/igem/BBa_J06001/annotation1512838/range1512838/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_J06001/annotation1512838/range1512838/1
type: Range
persistent identity: range1512838
display id: range1512838
version: 1
direction: direction/forward
top level: Stalker
start: 1
end: 54
orientation: inline

BBa_C0061
type: SequenceAnnotation
persistent identity: annotation1512856
display id: annotation1512856
version: 1
top level: Stalker
location: https://synbiohub.org/public/igem/BBa_J06001/annotation1512856/range1512856/1
component: BBa_C0061

https://synbiohub.org/public/igem/BBa_J06001/annotation1512856/range1512856/1
type: Range
persistent identity: range1512856
display id: range1512856
version: 1
direction: direction/forward
top level: Stalker
start: 81
end: 698
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1512873
display id: annotation1512873
version: 1
top level: Stalker
location: https://synbiohub.org/public/igem/BBa_J06001/annotation1512873/range1512873/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_J06001/annotation1512873/range1512873/1
type: Range
persistent identity: range1512873
display id: range1512873
version: 1
direction: direction/forward
top level: Stalker
start: 820
end: 860
orientation: inline

BBa_I729004
type: ComponentDefinition
persistent identity: BBa I729004
display id: BBa_I729004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Sensitive AHL Receiver
creator: Hiroki Fukutomi
created: 2007-10-20T11:00:00Z
modified: 2015-08-31T04:07:55Z
discontinued: false
dominant: false
experience: experience/None
group u list: _117_
m user id: 0
owner id: 1585
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This AHL receiver contains mutant LuxR. it can express GFP 0.1x AHL conc. compared wild type.
mutable provenance: Site direct mutagenesis -> Ligation with Biobrick
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_I729001
component: BBa_B0034
component: BBa_B0010
component: BBa_B0032
component: BBa_R0062
component: BBa_B0012
component: BBa_R0040
component: BBa_E0040
sequence annotation: BBa_I729001
sequence annotation: BBa_R0040
sequence annotation: BBa_B0034
sequence annotation: BBa_B0010
sequence annotation: BBa_R0062
sequence annotation: BBa_B0012
sequence annotation: BBa_B0032
sequence annotation: BBa_E0040
sequence: https://synbiohub.org/public/igem/BBa_I729004_sequence/1

BBa_I729001
type: Component
persistent identity: component1951541
display id: component1951541
version: 1
top level: BBa_I729004
access: public
definition: https://synbiohub.org/public/igem/BBa_I729001/1

BBa_B0034
type: Component
persistent identity: component1951540
display id: component1951540
version: 1
top level: BBa_I729004
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0010
type: Component
persistent identity: component1951542
display id: component1951542
version: 1
top level: BBa_I729004
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0010
type: Component
persistent identity: component1951558
display id: component1951558
version: 1
top level: BBa_I729004
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0032
type: Component
persistent identity: component1951554
display id: component1951554
version: 1
top level: BBa_I729004
access: public
definition: https://synbiohub.org/public/igem/BBa_B0032/1

BBa_R0062
type: Component
persistent identity: component1951549
display id: component1951549
version: 1
top level: BBa_I729004
access: public
definition: https://synbiohub.org/public/igem/BBa_R0062/1

BBa_B0012
type: Component
persistent identity: component1951544
display id: component1951544
version: 1
top level: BBa_I729004
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_R0040
type: Component
persistent identity: component1951535
display id: component1951535
version: 1
top level: BBa_I729004
access: public
definition: https://synbiohub.org/public/igem/BBa_R0040/1

BBa_E0040
type: Component
persistent identity: component1951557
display id: component1951557
version: 1
top level: BBa_I729004
access: public
definition: https://synbiohub.org/public/igem/BBa_E0040/1

BBa_B0012
type: Component
persistent identity: component1951560
display id: component1951560
version: 1
top level: BBa_I729004
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_I729001
type: SequenceAnnotation
persistent identity: annotation1951541
display id: annotation1951541
version: 1
top level: BBa_I729004
location: https://synbiohub.org/public/igem/BBa_I729004/annotation1951541/range1951541/1
component: BBa_I729001

https://synbiohub.org/public/igem/BBa_I729004/annotation1951541/range1951541/1
type: Range
persistent identity: range1951541
display id: range1951541
version: 1
direction: direction/reverse
top level: BBa_I729004
start: 81
end: 836
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1951535
display id: annotation1951535
version: 1
top level: BBa_I729004
location: https://synbiohub.org/public/igem/BBa_I729004/annotation1951535/range1951535/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_I729004/annotation1951535/range1951535/1
type: Range
persistent identity: range1951535
display id: range1951535
version: 1
direction: direction/forward
top level: BBa_I729004
start: 1
end: 54
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1951540
display id: annotation1951540
version: 1
top level: BBa_I729004
location: https://synbiohub.org/public/igem/BBa_I729004/annotation1951540/range1951540/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I729004/annotation1951540/range1951540/1
type: Range
persistent identity: range1951540
display id: range1951540
version: 1
direction: direction/reverse
top level: BBa_I729004
start: 63
end: 74
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1951542
display id: annotation1951542
version: 1
top level: BBa_I729004
location: https://synbiohub.org/public/igem/BBa_I729004/annotation1951542/range1951542/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I729004/annotation1951542/range1951542/1
type: Range
persistent identity: range1951542
display id: range1951542
version: 1
direction: direction/forward
top level: BBa_I729004
start: 845
end: 924
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1951558
display id: annotation1951558
version: 1
top level: BBa_I729004
location: https://synbiohub.org/public/igem/BBa_I729004/annotation1951558/range1951558/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I729004/annotation1951558/range1951558/1
type: Range
persistent identity: range1951558
display id: range1951558
version: 1
direction: direction/forward
top level: BBa_I729004
start: 1792
end: 1871
orientation: inline

BBa_R0062
type: SequenceAnnotation
persistent identity: annotation1951549
display id: annotation1951549
version: 1
top level: BBa_I729004
location: https://synbiohub.org/public/igem/BBa_I729004/annotation1951549/range1951549/1
component: BBa_R0062

https://synbiohub.org/public/igem/BBa_I729004/annotation1951549/range1951549/1
type: Range
persistent identity: range1951549
display id: range1951549
version: 1
direction: direction/forward
top level: BBa_I729004
start: 982
end: 1036
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1951544
display id: annotation1951544
version: 1
top level: BBa_I729004
location: https://synbiohub.org/public/igem/BBa_I729004/annotation1951544/range1951544/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I729004/annotation1951544/range1951544/1
type: Range
persistent identity: range1951544
display id: range1951544
version: 1
direction: direction/forward
top level: BBa_I729004
start: 933
end: 973
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation1951554
display id: annotation1951554
version: 1
top level: BBa_I729004
location: https://synbiohub.org/public/igem/BBa_I729004/annotation1951554/range1951554/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_I729004/annotation1951554/range1951554/1
type: Range
persistent identity: range1951554
display id: range1951554
version: 1
direction: direction/forward
top level: BBa_I729004
start: 1045
end: 1057
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1951560
display id: annotation1951560
version: 1
top level: BBa_I729004
location: https://synbiohub.org/public/igem/BBa_I729004/annotation1951560/range1951560/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I729004/annotation1951560/range1951560/1
type: Range
persistent identity: range1951560
display id: range1951560
version: 1
direction: direction/forward
top level: BBa_I729004
start: 1880
end: 1920
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation1951557
display id: annotation1951557
version: 1
top level: BBa_I729004
location: https://synbiohub.org/public/igem/BBa_I729004/annotation1951557/range1951557/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_I729004/annotation1951557/range1951557/1
type: Range
persistent identity: range1951557
display id: range1951557
version: 1
direction: direction/reverse
top level: BBa_I729004
start: 1064
end: 1783
orientation: inline

BBa_K081015
type: ComponentDefinition
persistent identity: BBa K081015
display id: BBa_K081015
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: luxI protein generator - PoPS->luxI
creator: Lorenzo Pasotti, Paolo Magni
created: 2008-10-18T11:00:00Z
modified: 2015-05-08T01:08:35Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _227_
m user id: 0
owner id: 2583
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: PoPS in -> luxI out. Strong RBS (efficiency=0.6). Artificial 39 bp terminator (efficiency=0.99).
mutable notes: We used BioBrick Standard Assembly.
mutable provenance: RBS: luxI: T:
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_C0061
component: BBa_B1006
component: BBa_B0030
sequence annotation: BBa_B1006
sequence annotation: BBa_B0030
sequence annotation: BBa_C0061
sequence: https://synbiohub.org/public/igem/BBa_K081015_sequence/1

BBa_C0061
type: Component
persistent identity: component2246002
display id: component2246002
version: 1
top level: BBa_K081015
access: public
definition: luxI

BBa_B1006
type: Component
persistent identity: component2246009
display id: component2246009
version: 1
top level: BBa_K081015
access: public
definition: https://synbiohub.org/public/igem/BBa_B1006/1

BBa_B0030
type: Component
persistent identity: component2245999
display id: component2245999
version: 1
top level: BBa_K081015
access: public
definition: https://synbiohub.org/public/igem/BBa_B0030/1

BBa_B1006
type: SequenceAnnotation
persistent identity: annotation2246009
display id: annotation2246009
version: 1
top level: BBa_K081015
location: https://synbiohub.org/public/igem/BBa_K081015/annotation2246009/range2246009/1
component: BBa_B1006

https://synbiohub.org/public/igem/BBa_K081015/annotation2246009/range2246009/1
type: Range
persistent identity: range2246009
display id: range2246009
version: 1
direction: direction/reverse
top level: BBa_K081015
start: 673
end: 711
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation2245999
display id: annotation2245999
version: 1
top level: BBa_K081015
location: https://synbiohub.org/public/igem/BBa_K081015/annotation2245999/range2245999/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_K081015/annotation2245999/range2245999/1
type: Range
persistent identity: range2245999
display id: range2245999
version: 1
direction: direction/forward
top level: BBa_K081015
start: 1
end: 15
orientation: inline

BBa_C0061
type: SequenceAnnotation
persistent identity: annotation2246002
display id: annotation2246002
version: 1
top level: BBa_K081015
location: https://synbiohub.org/public/igem/BBa_K081015/annotation2246002/range2246002/1
component: BBa_C0061

https://synbiohub.org/public/igem/BBa_K081015/annotation2246002/range2246002/1
type: Range
persistent identity: range2246002
display id: range2246002
version: 1
direction: direction/forward
top level: BBa_K081015
start: 22
end: 639
orientation: inline

BBa_K081008
type: ComponentDefinition
persistent identity: BBa K081008
display id: BBa_K081008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: luxI protein generator (TERM-)
creator: Lorenzo Pasotti, Paolo Magni
created: 2008-10-17T11:00:00Z
modified: 2015-05-08T01:08:35Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _227_
m user id: 0
owner id: 2583
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: luxI protein synthesizes 3OC6HSL, which binds to LuxR. Strong RBS (efficiency=0.6).
mutable notes: We used BioBrick Standard Assembly.
mutable provenance: luxI: RBS:
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_C0061
component: BBa_B0030
sequence annotation: BBa_C0061
sequence annotation: BBa_B0030
sequence: https://synbiohub.org/public/igem/BBa_K081008_sequence/1

BBa_C0061
type: Component
persistent identity: component2243991
display id: component2243991
version: 1
top level: BBa_K081008
access: public
definition: luxI

BBa_B0030
type: Component
persistent identity: component2243988
display id: component2243988
version: 1
top level: BBa_K081008
access: public
definition: https://synbiohub.org/public/igem/BBa_B0030/1

BBa_C0061
type: SequenceAnnotation
persistent identity: annotation2243991
display id: annotation2243991
version: 1
top level: BBa_K081008
location: https://synbiohub.org/public/igem/BBa_K081008/annotation2243991/range2243991/1
component: BBa_C0061

https://synbiohub.org/public/igem/BBa_K081008/annotation2243991/range2243991/1
type: Range
persistent identity: range2243991
display id: range2243991
version: 1
direction: direction/forward
top level: BBa_K081008
start: 22
end: 639
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation2243988
display id: annotation2243988
version: 1
top level: BBa_K081008
location: https://synbiohub.org/public/igem/BBa_K081008/annotation2243988/range2243988/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_K081008/annotation2243988/range2243988/1
type: Range
persistent identity: range2243988
display id: range2243988
version: 1
direction: direction/forward
top level: BBa_K081008
start: 1
end: 15
orientation: inline

BBa_K1520012
type: ComponentDefinition
persistent identity: BBa K1520012
display id: BBa_K1520012
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Prlux-rbs-rfp-rbs-luxR-Ter
creator: yue wu
created: 2014-09-21T11:00:00Z
modified: 2015-05-08T01:10:49Z
discontinued: false
dominant: false
group u list: _1902_
m user id: 0
owner id: 21318
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Prlux-rbs-rfp-rbs-luxR-Ter. It produces rfp and luxR when there is enough rfp and luxR.
mutable notes: None
mutable provenance: Prlux, rfp, luxR, Ter
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_R0062
component: BBa_K1520029
component: BBa_K1520501
component: BBa_K1520500
sequence annotation: BBa_K1520500
sequence annotation: BBa_K1520029
sequence annotation: BBa_R0062
sequence annotation: BBa_K1520501
sequence: https://synbiohub.org/public/igem/BBa_K1520012_sequence/1

BBa_R0062
type: Component
persistent identity: component2384968
display id: component2384968
version: 1
top level: BBa_K1520012
access: public
definition: https://synbiohub.org/public/igem/BBa_R0062/1

BBa_K1520029
type: Component
persistent identity: component2384977
display id: component2384977
version: 1
top level: BBa_K1520012
access: public
definition: https://synbiohub.org/public/igem/BBa_K1520029/1

BBa_K1520501
type: Component
persistent identity: component2384976
display id: component2384976
version: 1
top level: BBa_K1520012
access: public
definition: BBa_K1520501

BBa_K1520500
type: Component
persistent identity: component2384973
display id: component2384973
version: 1
top level: BBa_K1520012
access: public
definition: https://synbiohub.org/public/igem/BBa_K1520500/1

BBa_K1520500
type: SequenceAnnotation
persistent identity: annotation2384973
display id: annotation2384973
version: 1
top level: BBa_K1520012
location: https://synbiohub.org/public/igem/BBa_K1520012/annotation2384973/range2384973/1
component: BBa_K1520500

https://synbiohub.org/public/igem/BBa_K1520012/annotation2384973/range2384973/1
type: Range
persistent identity: range2384973
display id: range2384973
version: 1
direction: direction/reverse
top level: BBa_K1520012
start: 64
end: 795
orientation: inline

BBa_K1520029
type: SequenceAnnotation
persistent identity: annotation2384977
display id: annotation2384977
version: 1
top level: BBa_K1520012
location: https://synbiohub.org/public/igem/BBa_K1520012/annotation2384977/range2384977/1
component: BBa_K1520029

https://synbiohub.org/public/igem/BBa_K1520012/annotation2384977/range2384977/1
type: Range
persistent identity: range2384977
display id: range2384977
version: 1
direction: direction/reverse
top level: BBa_K1520012
start: 1611
end: 1739
orientation: inline

BBa_R0062
type: SequenceAnnotation
persistent identity: annotation2384968
display id: annotation2384968
version: 1
top level: BBa_K1520012
location: https://synbiohub.org/public/igem/BBa_K1520012/annotation2384968/range2384968/1
component: BBa_R0062

https://synbiohub.org/public/igem/BBa_K1520012/annotation2384968/range2384968/1
type: Range
persistent identity: range2384968
display id: range2384968
version: 1
direction: direction/forward
top level: BBa_K1520012
start: 1
end: 55
orientation: inline

BBa_K1520501
type: SequenceAnnotation
persistent identity: annotation2384976
display id: annotation2384976
version: 1
top level: BBa_K1520012
location: https://synbiohub.org/public/igem/BBa_K1520012/annotation2384976/range2384976/1
component: BBa_K1520501

https://synbiohub.org/public/igem/BBa_K1520012/annotation2384976/range2384976/1
type: Range
persistent identity: range2384976
display id: range2384976
version: 1
direction: direction/reverse
top level: BBa_K1520012
start: 804
end: 1602
orientation: inline

BBa_I13277
type: ComponentDefinition
persistent identity: BBa I13277
display id: BBa_I13277
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PoPS->PoPS amplifier (CinR-based, HSL driven)
creator: Debra Lin, Cell-Cell Signaling SMUG
created: 2004-08-08T11:00:00Z
modified: 2015-08-31T04:07:33Z
discontinued: false
dominant: false
experience: experience/None
group u list: _6_
m user id: 0
owner id: 101
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: CinR protein generator upstream of pCin. Useful device for cell-cell signaling. Expected to require HSL for maximum PoPS out.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Signalling
role: SO:0000804
component: BBa_B0010
component: BBa_B0034
component: BBa_R0078
component: BBa_C0077
component: BBa_B0012
sequence annotation: BBa_C0077
sequence annotation: BBa_B0010
sequence annotation: BBa_R0078
sequence annotation: BBa_B0034
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_I13277_sequence/1

BBa_B0010
type: Component
persistent identity: component1038924
display id: component1038924
version: 1
top level: BBa_I13277
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0034
type: Component
persistent identity: component1038908
display id: component1038908
version: 1
top level: BBa_I13277
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_R0078
type: Component
persistent identity: component1038948
display id: component1038948
version: 1
top level: BBa_I13277
access: public
definition: https://synbiohub.org/public/igem/BBa_R0078/1

BBa_C0077
type: Component
persistent identity: component1038918
display id: component1038918
version: 1
top level: BBa_I13277
access: public
definition: cinr

BBa_B0012
type: Component
persistent identity: component1038934
display id: component1038934
version: 1
top level: BBa_I13277
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_C0077
type: SequenceAnnotation
persistent identity: annotation1038918
display id: annotation1038918
version: 1
top level: BBa_I13277
location: https://synbiohub.org/public/igem/BBa_I13277/annotation1038918/range1038918/1
component: BBa_C0077

https://synbiohub.org/public/igem/BBa_I13277/annotation1038918/range1038918/1
type: Range
persistent identity: range1038918
display id: range1038918
version: 1
direction: direction/forward
top level: BBa_I13277
start: 19
end: 780
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1038924
display id: annotation1038924
version: 1
top level: BBa_I13277
location: https://synbiohub.org/public/igem/BBa_I13277/annotation1038924/range1038924/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I13277/annotation1038924/range1038924/1
type: Range
persistent identity: range1038924
display id: range1038924
version: 1
direction: direction/forward
top level: BBa_I13277
start: 814
end: 893
orientation: inline

BBa_R0078
type: SequenceAnnotation
persistent identity: annotation1038948
display id: annotation1038948
version: 1
top level: BBa_I13277
location: https://synbiohub.org/public/igem/BBa_I13277/annotation1038948/range1038948/1
component: BBa_R0078

https://synbiohub.org/public/igem/BBa_I13277/annotation1038948/range1038948/1
type: Range
persistent identity: range1038948
display id: range1038948
version: 1
direction: direction/forward
top level: BBa_I13277
start: 951
end: 1175
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1038908
display id: annotation1038908
version: 1
top level: BBa_I13277
location: https://synbiohub.org/public/igem/BBa_I13277/annotation1038908/range1038908/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I13277/annotation1038908/range1038908/1
type: Range
persistent identity: range1038908
display id: range1038908
version: 1
direction: direction/forward
top level: BBa_I13277
start: 1
end: 12
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1038934
display id: annotation1038934
version: 1
top level: BBa_I13277
location: https://synbiohub.org/public/igem/BBa_I13277/annotation1038934/range1038934/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I13277/annotation1038934/range1038934/1
type: Range
persistent identity: range1038934
display id: range1038934
version: 1
direction: direction/forward
top level: BBa_I13277
start: 902
end: 942
orientation: inline

lasI
type: ComponentDefinition
persistent identity: BBa C0078
display id: BBa_C0078
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: autoinducer synthetase for PAI from Pseudomonas aeruginosa
creator: Chris Walsh (Fighting Darwins)
created: 2004-01-27T12:00:00Z
modified: 2015-08-31T04:07:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: coding region for lasI protein, which produces the chemical signal AI-1
mutable provenance: www.ncbi.nlm.nih.gov
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Help:Barcodes
sequence annotation: LasI
sequence annotation: LVA
sequence: https://synbiohub.org/public/igem/BBa_C0078_sequence/1

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2214003
display id: annotation2214003
version: 1
top level: lasI
role: SO:0000807
role: feature/barcode
location: https://synbiohub.org/public/igem/BBa_C0078/annotation2214003/range2214003/1

https://synbiohub.org/public/igem/BBa_C0078/annotation2214003/range2214003/1
type: Range
persistent identity: range2214003
display id: range2214003
version: 1
direction: direction/forward
top level: lasI
start: 643
end: 667
orientation: inline

LasI
type: SequenceAnnotation
persistent identity: annotation305970
display id: annotation305970
version: 1
top level: lasI
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_C0078/annotation305970/range305970/1

https://synbiohub.org/public/igem/BBa_C0078/annotation305970/range305970/1
type: Range
persistent identity: range305970
display id: range305970
version: 1
direction: direction/forward
top level: lasI
start: 1
end: 603
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation306608
display id: annotation306608
version: 1
top level: lasI
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_C0078/annotation306608/range306608/1

https://synbiohub.org/public/igem/BBa_C0078/annotation306608/range306608/1
type: Range
persistent identity: range306608
display id: range306608
version: 1
direction: direction/forward
top level: lasI
start: 604
end: 636
orientation: inline

BBa_J07045
type: ComponentDefinition
persistent identity: BBa J07045
display id: BBa_J07045
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: J07014.B0015 (scFv 4M5.3.TT)
creator: Jenny Nguyen
created: 2005-08-02T11:00:00Z
modified: 2015-08-31T04:08:20Z
discontinued: false
dominant: false
group u list: _21_
m user id: 0
owner id: 350
owning group id: 21
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Anti-fluorescein scFv (4M5.3, 15 point mutations) with transcriptional terminator.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Signalling
role: SO:0000804
component: BBa_J07014
component: BBa_B0010
component: BBa_B0012
sequence annotation: BBa_J07014
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_J07045_sequence/1

BBa_J07014
type: Component
persistent identity: component1651375
display id: component1651375
version: 1
top level: BBa_J07045
access: public
definition: BBa_J07014

BBa_B0010
type: Component
persistent identity: component1651380
display id: component1651380
version: 1
top level: BBa_J07045
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
type: Component
persistent identity: component1651390
display id: component1651390
version: 1
top level: BBa_J07045
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_J07014
type: SequenceAnnotation
persistent identity: annotation1651375
display id: annotation1651375
version: 1
top level: BBa_J07045
location: https://synbiohub.org/public/igem/BBa_J07045/annotation1651375/range1651375/1
component: BBa_J07014

https://synbiohub.org/public/igem/BBa_J07045/annotation1651375/range1651375/1
type: Range
persistent identity: range1651375
display id: range1651375
version: 1
direction: direction/forward
top level: BBa_J07045
start: 1
end: 762
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1651380
display id: annotation1651380
version: 1
top level: BBa_J07045
location: https://synbiohub.org/public/igem/BBa_J07045/annotation1651380/range1651380/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_J07045/annotation1651380/range1651380/1
type: Range
persistent identity: range1651380
display id: range1651380
version: 1
direction: direction/forward
top level: BBa_J07045
start: 771
end: 850
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1651390
display id: annotation1651390
version: 1
top level: BBa_J07045
location: https://synbiohub.org/public/igem/BBa_J07045/annotation1651390/range1651390/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_J07045/annotation1651390/range1651390/1
type: Range
persistent identity: range1651390
display id: range1651390
version: 1
direction: direction/forward
top level: BBa_J07045
start: 859
end: 899
orientation: inline

BBa_K772001
type: ComponentDefinition
persistent identity: BBa K772001
display id: BBa_K772001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: EpCAM Binding Dock: C-terminus
creator: Mustafa Elitok
created: 2012-09-23T11:00:00Z
modified: 2015-05-08T01:13:15Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1024_
m user id: 0
owner id: 9573
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: This gene sequence includes an EpCAM (Epithelial Cell Adhesion Molecule) antigen binding domain, OmpA transmembrane protein (dock) and TEV protease C terminus. It is planned to use this subpart with BBa_772, which contains only TEV protease N terminus differently instead of C terminus, in order to induce a signal reaction in the case of EpCAM presence. EpCAM is an natural antigen which is found high significantly in the cases of some breast and colorectal cancer subtypes. On the upstream region of protein sequence, there are a constitutive pLac promoter and appropiate RBS. To see how this part works: Experience.
mutable notes: There are two protein sequences between domains and the dock: One between the binding domain (antibody) and dock and one between the part of TEV protease.
mutable provenance: CD225 (EpCAM antibody) is commonly used for the test of presence of EpCAM in the laboratories. The sequences of OmpA and TEV protease terminuses are acquired from the Registry.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
sequence annotation: CD215 Antibody
sequence annotation: Binding Dock
sequence annotation: TEV Protease C terminus
sequence annotation: OmpA (Dock)
sequence annotation: RBS
sequence annotation: pCons Promoter
sequence: https://synbiohub.org/public/igem/BBa_K772001_sequence/1

CD215 Antibody
type: SequenceAnnotation
persistent identity: annotation2203673
display id: annotation2203673
version: 1
top level: BBa_K772001
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K772001/annotation2203673/range2203673/1

https://synbiohub.org/public/igem/BBa_K772001/annotation2203673/range2203673/1
type: Range
persistent identity: range2203673
display id: range2203673
version: 1
direction: direction/forward
top level: BBa_K772001
start: 858
end: 1159
orientation: inline

Binding Dock
type: SequenceAnnotation
persistent identity: annotation2203674
display id: annotation2203674
version: 1
top level: BBa_K772001
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K772001/annotation2203674/range2203674/1

https://synbiohub.org/public/igem/BBa_K772001/annotation2203674/range2203674/1
type: Range
persistent identity: range2203674
display id: range2203674
version: 1
direction: direction/forward
top level: BBa_K772001
start: 94
end: 1159
orientation: inline

TEV Protease C terminus
type: SequenceAnnotation
persistent identity: annotation2203672
display id: annotation2203672
version: 1
top level: BBa_K772001
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K772001/annotation2203672/range2203672/1

https://synbiohub.org/public/igem/BBa_K772001/annotation2203672/range2203672/1
type: Range
persistent identity: range2203672
display id: range2203672
version: 1
direction: direction/forward
top level: BBa_K772001
start: 94
end: 419
orientation: inline

OmpA (Dock)
type: SequenceAnnotation
persistent identity: annotation2203671
display id: annotation2203671
version: 1
top level: BBa_K772001
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K772001/annotation2203671/range2203671/1

https://synbiohub.org/public/igem/BBa_K772001/annotation2203671/range2203671/1
type: Range
persistent identity: range2203671
display id: range2203671
version: 1
direction: direction/forward
top level: BBa_K772001
start: 420
end: 857
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2203670
display id: annotation2203670
version: 1
top level: BBa_K772001
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K772001/annotation2203670/range2203670/1

https://synbiohub.org/public/igem/BBa_K772001/annotation2203670/range2203670/1
type: Range
persistent identity: range2203670
display id: range2203670
version: 1
direction: direction/forward
top level: BBa_K772001
start: 73
end: 93
orientation: inline

pCons Promoter
type: SequenceAnnotation
persistent identity: annotation2203669
display id: annotation2203669
version: 1
top level: BBa_K772001
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K772001/annotation2203669/range2203669/1

https://synbiohub.org/public/igem/BBa_K772001/annotation2203669/range2203669/1
type: Range
persistent identity: range2203669
display id: range2203669
version: 1
direction: direction/forward
top level: BBa_K772001
start: 1
end: 72
orientation: inline

BBa_T9001
type: ComponentDefinition
persistent identity: BBa T9001
display id: BBa_T9001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: GFP and Producer Controlled by 3OC6HSL Receiver Device
creator: labnoa
created: 2005-06-20T11:00:00Z
modified: 2015-05-08T01:14:52Z
discontinued: false
dominant: true
experience: experience/None
group u list: _9_
m user id: 0
owner id: 371
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: The luxR based receiver, F2622 (formerly I13275 and I13280), controls the production of GFP. The GFP protein generator is the same as that found in I7101.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Signalling
role: SO:0000804
component: BBa_C0062
component: BBa_B0032
component: BBa_R0062
component: BBa_B0010
component: BBa_R0011
component: BBa_B0034
component: BBa_B0012
component: BBa_E0040
sequence annotation: BBa_C0062
sequence annotation: BBa_R0011
sequence annotation: BBa_E0040
sequence annotation: BBa_B0012
sequence annotation: BBa_B0010
sequence annotation: BBa_B0034
sequence annotation: BBa_B0032
sequence annotation: BBa_R0062
sequence: https://synbiohub.org/public/igem/BBa_T9001_sequence/1

BBa_C0062
type: Component
persistent identity: component1565087
display id: component1565087
version: 1
top level: BBa_T9001
access: public
definition: luxr

BBa_B0032
type: Component
persistent identity: component1565131
display id: component1565131
version: 1
top level: BBa_T9001
access: public
definition: https://synbiohub.org/public/igem/BBa_B0032/1

BBa_R0062
type: Component
persistent identity: component1565120
display id: component1565120
version: 1
top level: BBa_T9001
access: public
definition: https://synbiohub.org/public/igem/BBa_R0062/1

BBa_B0010
type: Component
persistent identity: component1565095
display id: component1565095
version: 1
top level: BBa_T9001
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_R0011
type: Component
persistent identity: component1565063
display id: component1565063
version: 1
top level: BBa_T9001
access: public
definition: https://synbiohub.org/public/igem/BBa_R0011/1

BBa_B0034
type: Component
persistent identity: component1565072
display id: component1565072
version: 1
top level: BBa_T9001
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0012
type: Component
persistent identity: component1565105
display id: component1565105
version: 1
top level: BBa_T9001
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0012
type: Component
persistent identity: component1565149
display id: component1565149
version: 1
top level: BBa_T9001
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_E0040
type: Component
persistent identity: component1565134
display id: component1565134
version: 1
top level: BBa_T9001
access: public
definition: https://synbiohub.org/public/igem/BBa_E0040/1

BBa_B0010
type: Component
persistent identity: component1565139
display id: component1565139
version: 1
top level: BBa_T9001
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_C0062
type: SequenceAnnotation
persistent identity: annotation1565087
display id: annotation1565087
version: 1
top level: BBa_T9001
location: https://synbiohub.org/public/igem/BBa_T9001/annotation1565087/range1565087/1
component: BBa_C0062

https://synbiohub.org/public/igem/BBa_T9001/annotation1565087/range1565087/1
type: Range
persistent identity: range1565087
display id: range1565087
version: 1
direction: direction/forward
top level: BBa_T9001
start: 82
end: 837
orientation: inline

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation1565063
display id: annotation1565063
version: 1
top level: BBa_T9001
location: https://synbiohub.org/public/igem/BBa_T9001/annotation1565063/range1565063/1
component: BBa_R0011

https://synbiohub.org/public/igem/BBa_T9001/annotation1565063/range1565063/1
type: Range
persistent identity: range1565063
display id: range1565063
version: 1
direction: direction/forward
top level: BBa_T9001
start: 1
end: 54
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation1565134
display id: annotation1565134
version: 1
top level: BBa_T9001
location: https://synbiohub.org/public/igem/BBa_T9001/annotation1565134/range1565134/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_T9001/annotation1565134/range1565134/1
type: Range
persistent identity: range1565134
display id: range1565134
version: 1
direction: direction/forward
top level: BBa_T9001
start: 1090
end: 1809
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1565149
display id: annotation1565149
version: 1
top level: BBa_T9001
location: https://synbiohub.org/public/igem/BBa_T9001/annotation1565149/range1565149/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_T9001/annotation1565149/range1565149/1
type: Range
persistent identity: range1565149
display id: range1565149
version: 1
direction: direction/forward
top level: BBa_T9001
start: 1906
end: 1946
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1565095
display id: annotation1565095
version: 1
top level: BBa_T9001
location: https://synbiohub.org/public/igem/BBa_T9001/annotation1565095/range1565095/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_T9001/annotation1565095/range1565095/1
type: Range
persistent identity: range1565095
display id: range1565095
version: 1
direction: direction/forward
top level: BBa_T9001
start: 871
end: 950
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1565072
display id: annotation1565072
version: 1
top level: BBa_T9001
location: https://synbiohub.org/public/igem/BBa_T9001/annotation1565072/range1565072/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_T9001/annotation1565072/range1565072/1
type: Range
persistent identity: range1565072
display id: range1565072
version: 1
direction: direction/forward
top level: BBa_T9001
start: 64
end: 75
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation1565131
display id: annotation1565131
version: 1
top level: BBa_T9001
location: https://synbiohub.org/public/igem/BBa_T9001/annotation1565131/range1565131/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_T9001/annotation1565131/range1565131/1
type: Range
persistent identity: range1565131
display id: range1565131
version: 1
direction: direction/forward
top level: BBa_T9001
start: 1071
end: 1083
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1565139
display id: annotation1565139
version: 1
top level: BBa_T9001
location: https://synbiohub.org/public/igem/BBa_T9001/annotation1565139/range1565139/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_T9001/annotation1565139/range1565139/1
type: Range
persistent identity: range1565139
display id: range1565139
version: 1
direction: direction/forward
top level: BBa_T9001
start: 1818
end: 1897
orientation: inline

BBa_R0062
type: SequenceAnnotation
persistent identity: annotation1565120
display id: annotation1565120
version: 1
top level: BBa_T9001
location: https://synbiohub.org/public/igem/BBa_T9001/annotation1565120/range1565120/1
component: BBa_R0062

https://synbiohub.org/public/igem/BBa_T9001/annotation1565120/range1565120/1
type: Range
persistent identity: range1565120
display id: range1565120
version: 1
direction: direction/forward
top level: BBa_T9001
start: 1008
end: 1062
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1565105
display id: annotation1565105
version: 1
top level: BBa_T9001
location: https://synbiohub.org/public/igem/BBa_T9001/annotation1565105/range1565105/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_T9001/annotation1565105/range1565105/1
type: Range
persistent identity: range1565105
display id: range1565105
version: 1
direction: direction/forward
top level: BBa_T9001
start: 959
end: 999
orientation: inline

rhlR
type: ComponentDefinition
persistent identity: BBa C0071
display id: BBa_C0071
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: rhlR repressor/activator from P. aeruginosa PA3477 (+LVA)
creator: Debra Lin, Srini Devadas, David Gray, Ronny Krashinsky, and Chris Zheng Liu, Boopers
created: 2004-01-26T12:00:00Z
modified: 2015-08-31T04:07:23Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Transcriptional regulator, in complex with N-butyryl-HSL, RhlR binds to the Rhl promoter
mutable notes: (1/28/04 Edit Jamboree) (0) No BB sites (1) ATG (2) TAATAA (3) Blast checks out (4) No codon changes (5) Maybe chassis compatability (check by experiment) (6) Codon usage not optimal (but looks OK) (7) ssrA tag added
mutable provenance: P. aeruginosa PA3477
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: rhlR
sequence annotation: Help:Barcodes
sequence annotation: LVA
sequence: https://synbiohub.org/public/igem/BBa_C0071_sequence/1

rhlR
type: SequenceAnnotation
persistent identity: annotation301192
display id: annotation301192
version: 1
top level: rhlR
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_C0071/annotation301192/range301192/1

https://synbiohub.org/public/igem/BBa_C0071/annotation301192/range301192/1
type: Range
persistent identity: range301192
display id: range301192
version: 1
direction: direction/forward
top level: rhlR
start: 1
end: 723
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213995
display id: annotation2213995
version: 1
top level: rhlR
role: SO:0000807
role: feature/barcode
location: https://synbiohub.org/public/igem/BBa_C0071/annotation2213995/range2213995/1

https://synbiohub.org/public/igem/BBa_C0071/annotation2213995/range2213995/1
type: Range
persistent identity: range2213995
display id: range2213995
version: 1
direction: direction/forward
top level: rhlR
start: 763
end: 787
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation306523
display id: annotation306523
version: 1
top level: rhlR
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_C0071/annotation306523/range306523/1

https://synbiohub.org/public/igem/BBa_C0071/annotation306523/range306523/1
type: Range
persistent identity: range306523
display id: range306523
version: 1
direction: direction/forward
top level: rhlR
start: 724
end: 756
orientation: inline

BBa_I13207
type: ComponentDefinition
persistent identity: BBa I13207
display id: BBa_I13207
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: HSL/aiiA test construct
creator: Barry Canton
created: 2004-07-31T11:00:00Z
modified: 2015-08-31T04:07:33Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_6_
m user id: 0
owner id: 135
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: LuxR receiver circuit (driven by ptet) with pBad controlling the expression of aiiA. Useful for measuring the ability of aiiA (LVA+) to turn off the response of a tet driven receiver cell to exogenous HSL levels
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Signalling
role: SO:0000804
component: BBa_B0010
component: BBa_C0060
component: BBa_B0012
component: BBa_C0062
component: BBa_E0030
component: BBa_B0034
component: BBa_R0062
component: BBa_R0040
component: BBa_I0500
sequence annotation: BBa_B0034
sequence annotation: BBa_E0030
sequence annotation: BBa_B0010
sequence annotation: BBa_C0060
sequence annotation: BBa_B0012
sequence annotation: BBa_R0040
sequence annotation: BBa_I0500
sequence annotation: BBa_R0062
sequence annotation: BBa_C0062
sequence: https://synbiohub.org/public/igem/BBa_I13207_sequence/1

BBa_B0010
type: Component
persistent identity: component1044059
display id: component1044059
version: 1
top level: BBa_I13207
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_C0060
type: Component
persistent identity: component1044134
display id: component1044134
version: 1
top level: BBa_I13207
access: public
definition: aiiA

BBa_B0012
type: Component
persistent identity: component1044152
display id: component1044152
version: 1
top level: BBa_I13207
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_C0062
type: Component
persistent identity: component1044051
display id: component1044051
version: 1
top level: BBa_I13207
access: public
definition: luxr

BBa_B0012
type: Component
persistent identity: component1044109
display id: component1044109
version: 1
top level: BBa_I13207
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_E0030
type: Component
persistent identity: component1044094
display id: component1044094
version: 1
top level: BBa_I13207
access: public
definition: https://synbiohub.org/public/igem/BBa_E0030/1

BBa_B0034
type: Component
persistent identity: component1044036
display id: component1044036
version: 1
top level: BBa_I13207
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0010
type: Component
persistent identity: component1044099
display id: component1044099
version: 1
top level: BBa_I13207
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_R0062
type: Component
persistent identity: component1044084
display id: component1044084
version: 1
top level: BBa_I13207
access: public
definition: https://synbiohub.org/public/igem/BBa_R0062/1

BBa_R0040
type: Component
persistent identity: component1044028
display id: component1044028
version: 1
top level: BBa_I13207
access: public
definition: https://synbiohub.org/public/igem/BBa_R0040/1

BBa_B0034
type: Component
persistent identity: component1044092
display id: component1044092
version: 1
top level: BBa_I13207
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0010
type: Component
persistent identity: component1044142
display id: component1044142
version: 1
top level: BBa_I13207
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_I0500
type: Component
persistent identity: component1044114
display id: component1044114
version: 1
top level: BBa_I13207
access: public
definition: https://synbiohub.org/public/igem/BBa_I0500/1

BBa_B0034
type: Component
persistent identity: component1044119
display id: component1044119
version: 1
top level: BBa_I13207
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0012
type: Component
persistent identity: component1044069
display id: component1044069
version: 1
top level: BBa_I13207
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1044119
display id: annotation1044119
version: 1
top level: BBa_I13207
location: https://synbiohub.org/public/igem/BBa_I13207/annotation1044119/range1044119/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I13207/annotation1044119/range1044119/1
type: Range
persistent identity: range1044119
display id: range1044119
version: 1
direction: direction/forward
top level: BBa_I13207
start: 3174
end: 3185
orientation: inline

BBa_E0030
type: SequenceAnnotation
persistent identity: annotation1044094
display id: annotation1044094
version: 1
top level: BBa_I13207
location: https://synbiohub.org/public/igem/BBa_I13207/annotation1044094/range1044094/1
component: BBa_E0030

https://synbiohub.org/public/igem/BBa_I13207/annotation1044094/range1044094/1
type: Range
persistent identity: range1044094
display id: range1044094
version: 1
direction: direction/forward
top level: BBa_I13207
start: 1088
end: 1810
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1044099
display id: annotation1044099
version: 1
top level: BBa_I13207
location: https://synbiohub.org/public/igem/BBa_I13207/annotation1044099/range1044099/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I13207/annotation1044099/range1044099/1
type: Range
persistent identity: range1044099
display id: range1044099
version: 1
direction: direction/forward
top level: BBa_I13207
start: 1819
end: 1898
orientation: inline

BBa_C0060
type: SequenceAnnotation
persistent identity: annotation1044134
display id: annotation1044134
version: 1
top level: BBa_I13207
location: https://synbiohub.org/public/igem/BBa_I13207/annotation1044134/range1044134/1
component: BBa_C0060

https://synbiohub.org/public/igem/BBa_I13207/annotation1044134/range1044134/1
type: Range
persistent identity: range1044134
display id: range1044134
version: 1
direction: direction/forward
top level: BBa_I13207
start: 3192
end: 3980
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1044069
display id: annotation1044069
version: 1
top level: BBa_I13207
location: https://synbiohub.org/public/igem/BBa_I13207/annotation1044069/range1044069/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I13207/annotation1044069/range1044069/1
type: Range
persistent identity: range1044069
display id: range1044069
version: 1
direction: direction/forward
top level: BBa_I13207
start: 958
end: 998
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1044028
display id: annotation1044028
version: 1
top level: BBa_I13207
location: https://synbiohub.org/public/igem/BBa_I13207/annotation1044028/range1044028/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_I13207/annotation1044028/range1044028/1
type: Range
persistent identity: range1044028
display id: range1044028
version: 1
direction: direction/forward
top level: BBa_I13207
start: 1
end: 54
orientation: inline

BBa_I0500
type: SequenceAnnotation
persistent identity: annotation1044114
display id: annotation1044114
version: 1
top level: BBa_I13207
location: https://synbiohub.org/public/igem/BBa_I13207/annotation1044114/range1044114/1
component: BBa_I0500

https://synbiohub.org/public/igem/BBa_I13207/annotation1044114/range1044114/1
type: Range
persistent identity: range1044114
display id: range1044114
version: 1
direction: direction/forward
top level: BBa_I13207
start: 1956
end: 3165
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1044109
display id: annotation1044109
version: 1
top level: BBa_I13207
location: https://synbiohub.org/public/igem/BBa_I13207/annotation1044109/range1044109/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I13207/annotation1044109/range1044109/1
type: Range
persistent identity: range1044109
display id: range1044109
version: 1
direction: direction/forward
top level: BBa_I13207
start: 1907
end: 1947
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1044152
display id: annotation1044152
version: 1
top level: BBa_I13207
location: https://synbiohub.org/public/igem/BBa_I13207/annotation1044152/range1044152/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I13207/annotation1044152/range1044152/1
type: Range
persistent identity: range1044152
display id: range1044152
version: 1
direction: direction/forward
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end: 4142
orientation: inline

BBa_R0062
type: SequenceAnnotation
persistent identity: annotation1044084
display id: annotation1044084
version: 1
top level: BBa_I13207
location: https://synbiohub.org/public/igem/BBa_I13207/annotation1044084/range1044084/1
component: BBa_R0062

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persistent identity: range1044084
display id: range1044084
version: 1
direction: direction/forward
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end: 1061
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1044059
display id: annotation1044059
version: 1
top level: BBa_I13207
location: https://synbiohub.org/public/igem/BBa_I13207/annotation1044059/range1044059/1
component: BBa_B0010

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type: Range
persistent identity: range1044059
display id: range1044059
version: 1
direction: direction/forward
top level: BBa_I13207
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end: 949
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1044036
display id: annotation1044036
version: 1
top level: BBa_I13207
location: https://synbiohub.org/public/igem/BBa_I13207/annotation1044036/range1044036/1
component: BBa_B0034

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persistent identity: range1044036
display id: range1044036
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BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1044142
display id: annotation1044142
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top level: BBa_I13207
location: https://synbiohub.org/public/igem/BBa_I13207/annotation1044142/range1044142/1
component: BBa_B0010

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type: Range
persistent identity: range1044142
display id: range1044142
version: 1
direction: direction/forward
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end: 4093
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1044092
display id: annotation1044092
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location: https://synbiohub.org/public/igem/BBa_I13207/annotation1044092/range1044092/1
component: BBa_B0034

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display id: range1044092
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orientation: inline

BBa_C0062
type: SequenceAnnotation
persistent identity: annotation1044051
display id: annotation1044051
version: 1
top level: BBa_I13207
location: https://synbiohub.org/public/igem/BBa_I13207/annotation1044051/range1044051/1
component: BBa_C0062

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type: Range
persistent identity: range1044051
display id: range1044051
version: 1
direction: direction/forward
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BBa_T9002
type: ComponentDefinition
persistent identity: BBa T9002
display id: BBa_T9002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: GFP Producer Controlled by 3OC6HSL Receiver Device
creator: Anna Labno
created: 2005-06-20T11:00:00Z
modified: 2015-05-08T01:14:52Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _101_
m user id: 0
owner id: 371
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: The luxR based receiver, F2620 (formerly I13270), controls the production of GFP. The GFP protein generator is the same as that found in I7101.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Signalling
role: SO:0000804
component: BBa_R0062
component: BBa_R0040
component: BBa_C0062
component: BBa_B0034
component: BBa_B0012
component: BBa_B0010
component: BBa_E0040
component: BBa_B0032
sequence annotation: BBa_B0010
sequence annotation: BBa_R0040
sequence annotation: BBa_B0034
sequence annotation: BBa_C0062
sequence annotation: BBa_E0040
sequence annotation: BBa_B0012
sequence annotation: BBa_B0032
sequence annotation: BBa_R0062
sequence: https://synbiohub.org/public/igem/BBa_T9002_sequence/1

BBa_R0062
type: Component
persistent identity: component1565221
display id: component1565221
version: 1
top level: BBa_T9002
access: public
definition: https://synbiohub.org/public/igem/BBa_R0062/1

BBa_R0040
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display id: component1565165
version: 1
top level: BBa_T9002
access: public
definition: https://synbiohub.org/public/igem/BBa_R0040/1

BBa_C0062
type: Component
persistent identity: component1565188
display id: component1565188
version: 1
top level: BBa_T9002
access: public
definition: luxr

BBa_B0034
type: Component
persistent identity: component1565173
display id: component1565173
version: 1
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access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0012
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display id: component1565206
version: 1
top level: BBa_T9002
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0012
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display id: component1565250
version: 1
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access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0010
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display id: component1565240
version: 1
top level: BBa_T9002
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_E0040
type: Component
persistent identity: component1565235
display id: component1565235
version: 1
top level: BBa_T9002
access: public
definition: https://synbiohub.org/public/igem/BBa_E0040/1

BBa_B0032
type: Component
persistent identity: component1565232
display id: component1565232
version: 1
top level: BBa_T9002
access: public
definition: https://synbiohub.org/public/igem/BBa_B0032/1

BBa_B0010
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persistent identity: component1565196
display id: component1565196
version: 1
top level: BBa_T9002
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0010
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persistent identity: annotation1565196
display id: annotation1565196
version: 1
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component: BBa_B0010

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display id: range1565196
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direction: direction/forward
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end: 949
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1565165
display id: annotation1565165
version: 1
top level: BBa_T9002
location: https://synbiohub.org/public/igem/BBa_T9002/annotation1565165/range1565165/1
component: BBa_R0040

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type: Range
persistent identity: range1565165
display id: range1565165
version: 1
direction: direction/forward
top level: BBa_T9002
start: 1
end: 54
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1565173
display id: annotation1565173
version: 1
top level: BBa_T9002
location: https://synbiohub.org/public/igem/BBa_T9002/annotation1565173/range1565173/1
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type: Range
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direction: direction/forward
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end: 74
orientation: inline

BBa_C0062
type: SequenceAnnotation
persistent identity: annotation1565188
display id: annotation1565188
version: 1
top level: BBa_T9002
location: https://synbiohub.org/public/igem/BBa_T9002/annotation1565188/range1565188/1
component: BBa_C0062

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type: Range
persistent identity: range1565188
display id: range1565188
version: 1
direction: direction/forward
top level: BBa_T9002
start: 81
end: 836
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation1565235
display id: annotation1565235
version: 1
top level: BBa_T9002
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component: BBa_E0040

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type: Range
persistent identity: range1565235
display id: range1565235
version: 1
direction: direction/forward
top level: BBa_T9002
start: 1089
end: 1808
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1565250
display id: annotation1565250
version: 1
top level: BBa_T9002
location: https://synbiohub.org/public/igem/BBa_T9002/annotation1565250/range1565250/1
component: BBa_B0012

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type: Range
persistent identity: range1565250
display id: range1565250
version: 1
direction: direction/forward
top level: BBa_T9002
start: 1905
end: 1945
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation1565232
display id: annotation1565232
version: 1
top level: BBa_T9002
location: https://synbiohub.org/public/igem/BBa_T9002/annotation1565232/range1565232/1
component: BBa_B0032

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type: Range
persistent identity: range1565232
display id: range1565232
version: 1
direction: direction/forward
top level: BBa_T9002
start: 1070
end: 1082
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1565240
display id: annotation1565240
version: 1
top level: BBa_T9002
location: https://synbiohub.org/public/igem/BBa_T9002/annotation1565240/range1565240/1
component: BBa_B0010

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type: Range
persistent identity: range1565240
display id: range1565240
version: 1
direction: direction/forward
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start: 1817
end: 1896
orientation: inline

BBa_B0012
type: SequenceAnnotation
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display id: annotation1565206
version: 1
top level: BBa_T9002
location: https://synbiohub.org/public/igem/BBa_T9002/annotation1565206/range1565206/1
component: BBa_B0012

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persistent identity: range1565206
display id: range1565206
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direction: direction/forward
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start: 958
end: 998
orientation: inline

BBa_R0062
type: SequenceAnnotation
persistent identity: annotation1565221
display id: annotation1565221
version: 1
top level: BBa_T9002
location: https://synbiohub.org/public/igem/BBa_T9002/annotation1565221/range1565221/1
component: BBa_R0062

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type: Range
persistent identity: range1565221
display id: range1565221
version: 1
direction: direction/forward
top level: BBa_T9002
start: 1007
end: 1061
orientation: inline

BBa_I13211
type: ComponentDefinition
persistent identity: BBa I13211
display id: BBa_I13211
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Biobricked version of the natural Lux quorum sensing system
creator: Barry Canton
created: 2004-08-04T11:00:00Z
modified: 2015-08-31T04:07:33Z
discontinued: false
dominant: true
experience: experience/None
group u list: _11_6_
m user id: 0
owner id: 135
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This device includes a positive feedback loop where LuxI production produces 3OC6HSL that binds to LuxR and activates transcription from R0062 producing more LuxI. There is no output from this device, it must be combined with another device, controlled by R0062 to produce output.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Signalling
role: SO:0000804
component: BBa_B0010
component: BBa_R0062
component: BBa_B0034
component: BBa_B0012
component: BBa_C0062
component: BBa_R0063
component: BBa_C0061
sequence annotation: BBa_B0034
sequence annotation: BBa_B0012
sequence annotation: BBa_B0010
sequence annotation: BBa_R0063
sequence annotation: BBa_C0061
sequence annotation: BBa_R0062
sequence annotation: BBa_C0062
sequence: https://synbiohub.org/public/igem/BBa_I13211_sequence/1

BBa_B0010
type: Component
persistent identity: component1030400
display id: component1030400
version: 1
top level: BBa_I13211
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_R0062
type: Component
persistent identity: component1030425
display id: component1030425
version: 1
top level: BBa_I13211
access: public
definition: https://synbiohub.org/public/igem/BBa_R0062/1

BBa_B0034
type: Component
persistent identity: component1030377
display id: component1030377
version: 1
top level: BBa_I13211
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0012
type: Component
persistent identity: component1030410
display id: component1030410
version: 1
top level: BBa_I13211
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_C0062
type: Component
persistent identity: component1030392
display id: component1030392
version: 1
top level: BBa_I13211
access: public
definition: luxr

BBa_R0063
type: Component
persistent identity: component1030368
display id: component1030368
version: 1
top level: BBa_I13211
access: public
definition: https://synbiohub.org/public/igem/BBa_R0063/1

BBa_B0034
type: Component
persistent identity: component1030433
display id: component1030433
version: 1
top level: BBa_I13211
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0010
type: Component
persistent identity: component1030450
display id: component1030450
version: 1
top level: BBa_I13211
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
type: Component
persistent identity: component1030460
display id: component1030460
version: 1
top level: BBa_I13211
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_C0061
type: Component
persistent identity: component1030443
display id: component1030443
version: 1
top level: BBa_I13211
access: public
definition: luxI

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1030433
display id: annotation1030433
version: 1
top level: BBa_I13211
location: https://synbiohub.org/public/igem/BBa_I13211/annotation1030433/range1030433/1
component: BBa_B0034

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type: Range
persistent identity: range1030433
display id: range1030433
version: 1
direction: direction/forward
top level: BBa_I13211
start: 1167
end: 1178
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1030460
display id: annotation1030460
version: 1
top level: BBa_I13211
location: https://synbiohub.org/public/igem/BBa_I13211/annotation1030460/range1030460/1
component: BBa_B0012

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type: Range
persistent identity: range1030460
display id: range1030460
version: 1
direction: direction/forward
top level: BBa_I13211
start: 1924
end: 1964
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1030400
display id: annotation1030400
version: 1
top level: BBa_I13211
location: https://synbiohub.org/public/igem/BBa_I13211/annotation1030400/range1030400/1
component: BBa_B0010

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type: Range
persistent identity: range1030400
display id: range1030400
version: 1
direction: direction/forward
top level: BBa_I13211
start: 967
end: 1046
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1030377
display id: annotation1030377
version: 1
top level: BBa_I13211
location: https://synbiohub.org/public/igem/BBa_I13211/annotation1030377/range1030377/1
component: BBa_B0034

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type: Range
persistent identity: range1030377
display id: range1030377
version: 1
direction: direction/forward
top level: BBa_I13211
start: 160
end: 171
orientation: inline

BBa_R0063
type: SequenceAnnotation
persistent identity: annotation1030368
display id: annotation1030368
version: 1
top level: BBa_I13211
location: https://synbiohub.org/public/igem/BBa_I13211/annotation1030368/range1030368/1
component: BBa_R0063

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type: Range
persistent identity: range1030368
display id: range1030368
version: 1
direction: direction/forward
top level: BBa_I13211
start: 1
end: 151
orientation: inline

BBa_C0061
type: SequenceAnnotation
persistent identity: annotation1030443
display id: annotation1030443
version: 1
top level: BBa_I13211
location: https://synbiohub.org/public/igem/BBa_I13211/annotation1030443/range1030443/1
component: BBa_C0061

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type: Range
persistent identity: range1030443
display id: range1030443
version: 1
direction: direction/forward
top level: BBa_I13211
start: 1185
end: 1802
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1030410
display id: annotation1030410
version: 1
top level: BBa_I13211
location: https://synbiohub.org/public/igem/BBa_I13211/annotation1030410/range1030410/1
component: BBa_B0012

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type: Range
persistent identity: range1030410
display id: range1030410
version: 1
direction: direction/forward
top level: BBa_I13211
start: 1055
end: 1095
orientation: inline

BBa_R0062
type: SequenceAnnotation
persistent identity: annotation1030425
display id: annotation1030425
version: 1
top level: BBa_I13211
location: https://synbiohub.org/public/igem/BBa_I13211/annotation1030425/range1030425/1
component: BBa_R0062

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type: Range
persistent identity: range1030425
display id: range1030425
version: 1
direction: direction/forward
top level: BBa_I13211
start: 1104
end: 1158
orientation: inline

BBa_C0062
type: SequenceAnnotation
persistent identity: annotation1030392
display id: annotation1030392
version: 1
top level: BBa_I13211
location: https://synbiohub.org/public/igem/BBa_I13211/annotation1030392/range1030392/1
component: BBa_C0062

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type: Range
persistent identity: range1030392
display id: range1030392
version: 1
direction: direction/forward
top level: BBa_I13211
start: 178
end: 933
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1030450
display id: annotation1030450
version: 1
top level: BBa_I13211
location: https://synbiohub.org/public/igem/BBa_I13211/annotation1030450/range1030450/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I13211/annotation1030450/range1030450/1
type: Range
persistent identity: range1030450
display id: range1030450
version: 1
direction: direction/forward
top level: BBa_I13211
start: 1836
end: 1915
orientation: inline

BBa_F2622
type: ComponentDefinition
persistent identity: BBa F2622
display id: BBa_F2622
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: 3OC6HSL Receiver Device
creator: Barry Canton
created: 2004-12-14T12:00:00Z
modified: 2015-08-31T04:07:27Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _11_6_
m user id: 0
owner id: 135
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This device senses the 3OC6HSL concentration in the media and produces output PoPS according to the transfer function given below. LuxR production is controlled by the lac repressible R0011.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Signalling
role: SO:0000804
component: BBa_R0011
component: BBa_C0062
component: BBa_B0012
component: BBa_B0010
component: BBa_R0062
component: BBa_B0034
sequence annotation: BBa_B0012
sequence annotation: BBa_B0034
sequence annotation: BBa_R0011
sequence annotation: BBa_C0062
sequence annotation: BBa_R0062
sequence annotation: BBa_B0010
sequence: https://synbiohub.org/public/igem/BBa_F2622_sequence/1

BBa_R0011
type: Component
persistent identity: component1484639
display id: component1484639
version: 1
top level: BBa_F2622
access: public
definition: https://synbiohub.org/public/igem/BBa_R0011/1

BBa_C0062
type: Component
persistent identity: component1484663
display id: component1484663
version: 1
top level: BBa_F2622
access: public
definition: luxr

BBa_B0012
type: Component
persistent identity: component1484681
display id: component1484681
version: 1
top level: BBa_F2622
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0010
type: Component
persistent identity: component1484671
display id: component1484671
version: 1
top level: BBa_F2622
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_R0062
type: Component
persistent identity: component1484696
display id: component1484696
version: 1
top level: BBa_F2622
access: public
definition: https://synbiohub.org/public/igem/BBa_R0062/1

BBa_B0034
type: Component
persistent identity: component1484648
display id: component1484648
version: 1
top level: BBa_F2622
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1484681
display id: annotation1484681
version: 1
top level: BBa_F2622
location: https://synbiohub.org/public/igem/BBa_F2622/annotation1484681/range1484681/1
component: BBa_B0012

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type: Range
persistent identity: range1484681
display id: range1484681
version: 1
direction: direction/forward
top level: BBa_F2622
start: 959
end: 999
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1484648
display id: annotation1484648
version: 1
top level: BBa_F2622
location: https://synbiohub.org/public/igem/BBa_F2622/annotation1484648/range1484648/1
component: BBa_B0034

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type: Range
persistent identity: range1484648
display id: range1484648
version: 1
direction: direction/forward
top level: BBa_F2622
start: 64
end: 75
orientation: inline

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation1484639
display id: annotation1484639
version: 1
top level: BBa_F2622
location: https://synbiohub.org/public/igem/BBa_F2622/annotation1484639/range1484639/1
component: BBa_R0011

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type: Range
persistent identity: range1484639
display id: range1484639
version: 1
direction: direction/forward
top level: BBa_F2622
start: 1
end: 54
orientation: inline

BBa_C0062
type: SequenceAnnotation
persistent identity: annotation1484663
display id: annotation1484663
version: 1
top level: BBa_F2622
location: https://synbiohub.org/public/igem/BBa_F2622/annotation1484663/range1484663/1
component: BBa_C0062

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type: Range
persistent identity: range1484663
display id: range1484663
version: 1
direction: direction/forward
top level: BBa_F2622
start: 82
end: 837
orientation: inline

BBa_R0062
type: SequenceAnnotation
persistent identity: annotation1484696
display id: annotation1484696
version: 1
top level: BBa_F2622
location: https://synbiohub.org/public/igem/BBa_F2622/annotation1484696/range1484696/1
component: BBa_R0062

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type: Range
persistent identity: range1484696
display id: range1484696
version: 1
direction: direction/forward
top level: BBa_F2622
start: 1008
end: 1062
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1484671
display id: annotation1484671
version: 1
top level: BBa_F2622
location: https://synbiohub.org/public/igem/BBa_F2622/annotation1484671/range1484671/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_F2622/annotation1484671/range1484671/1
type: Range
persistent identity: range1484671
display id: range1484671
version: 1
direction: direction/forward
top level: BBa_F2622
start: 871
end: 950
orientation: inline

BBa_K332032
type: ComponentDefinition
persistent identity: BBa K332032
display id: BBa_K332032
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Constitutive promoter+37℃ induced RBS+tetR+double terminator+Ptet+RBS+GFP+double terminator
creator: KUO, CHIA-CHUN
created: 2010-10-21T11:00:00Z
modified: 2015-05-08T01:12:05Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _452_
m user id: 0
owner id: 7059
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: We want to utilize a temperature-regulated mechanism to control the expression period of a crystal protein. Therefore, we took advantage of the interaction between 37?? C induced RBS, tetR gene and pTet promoter to create the temperature regulating circuit. The following is a description of our prescribed strategy. First, we use the constitutive promoter, 37?? C induced RBS (K115002), the tetR gene and terminators. We will call this part the ???Regulator???.
mutable notes: none
mutable provenance: none
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_E0040
component: BBa_K332031
component: BBa_R0040
component: BBa_B0034
component: BBa_J61048
sequence annotation: BBa_R0040
sequence annotation: BBa_K332031
sequence annotation: BBa_B0034
sequence annotation: BBa_E0040
sequence annotation: BBa_J61048
sequence: https://synbiohub.org/public/igem/BBa_K332032_sequence/1

BBa_E0040
type: Component
persistent identity: component2273255
display id: component2273255
version: 1
top level: BBa_K332032
access: public
definition: https://synbiohub.org/public/igem/BBa_E0040/1

BBa_K332031
type: Component
persistent identity: component2273246
display id: component2273246
version: 1
top level: BBa_K332032
access: public
definition: BBa_K332031

BBa_R0040
type: Component
persistent identity: component2273247
display id: component2273247
version: 1
top level: BBa_K332032
access: public
definition: https://synbiohub.org/public/igem/BBa_R0040/1

BBa_B0034
type: Component
persistent identity: component2273253
display id: component2273253
version: 1
top level: BBa_K332032
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_J61048
type: Component
persistent identity: component2273256
display id: component2273256
version: 1
top level: BBa_K332032
access: public
definition: https://synbiohub.org/public/igem/BBa_J61048/1

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2273247
display id: annotation2273247
version: 1
top level: BBa_K332032
location: https://synbiohub.org/public/igem/BBa_K332032/annotation2273247/range2273247/1
component: BBa_R0040

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type: Range
persistent identity: range2273247
display id: range2273247
version: 1
direction: direction/forward
top level: BBa_K332032
start: 916
end: 969
orientation: inline

BBa_K332031
type: SequenceAnnotation
persistent identity: annotation2273246
display id: annotation2273246
version: 1
top level: BBa_K332032
location: https://synbiohub.org/public/igem/BBa_K332032/annotation2273246/range2273246/1
component: BBa_K332031

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type: Range
persistent identity: range2273246
display id: range2273246
version: 1
direction: direction/reverse
top level: BBa_K332032
start: 1
end: 907
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2273253
display id: annotation2273253
version: 1
top level: BBa_K332032
location: https://synbiohub.org/public/igem/BBa_K332032/annotation2273253/range2273253/1
component: BBa_B0034

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type: Range
persistent identity: range2273253
display id: range2273253
version: 1
direction: direction/reverse
top level: BBa_K332032
start: 978
end: 989
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation2273255
display id: annotation2273255
version: 1
top level: BBa_K332032
location: https://synbiohub.org/public/igem/BBa_K332032/annotation2273255/range2273255/1
component: BBa_E0040

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type: Range
persistent identity: range2273255
display id: range2273255
version: 1
direction: direction/reverse
top level: BBa_K332032
start: 996
end: 1715
orientation: inline

BBa_J61048
type: SequenceAnnotation
persistent identity: annotation2273256
display id: annotation2273256
version: 1
top level: BBa_K332032
location: https://synbiohub.org/public/igem/BBa_K332032/annotation2273256/range2273256/1
component: BBa_J61048

https://synbiohub.org/public/igem/BBa_K332032/annotation2273256/range2273256/1
type: Range
persistent identity: range2273256
display id: range2273256
version: 1
direction: direction/reverse
top level: BBa_K332032
start: 1724
end: 1836
orientation: inline

BBa_K082036
type: ComponentDefinition
persistent identity: BBa K082036
display id: BBa_K082036
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LuxR & RhlI generator
creator: Zhen Xu , Binjie Xu
created: 2008-10-28T12:00:00Z
modified: 2015-05-08T01:08:36Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _228_
m user id: 0
owner id: 1560
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: none
mutable notes: none
mutable provenance: none
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0015
component: BBa_B0034
component: BBa_C0070
component: BBa_R0040
component: BBa_I0462
sequence annotation: BBa_R0040
sequence annotation: BBa_C0070
sequence annotation: BBa_B0015
sequence annotation: BBa_B0034
sequence annotation: BBa_I0462
sequence: https://synbiohub.org/public/igem/BBa_K082036_sequence/1

BBa_B0015
type: Component
persistent identity: component2282686
display id: component2282686
version: 1
top level: BBa_K082036
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_B0034
type: Component
persistent identity: component2282675
display id: component2282675
version: 1
top level: BBa_K082036
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_C0070
type: Component
persistent identity: component2282679
display id: component2282679
version: 1
top level: BBa_K082036
access: public
definition: rhII

BBa_R0040
type: Component
persistent identity: component2282669
display id: component2282669
version: 1
top level: BBa_K082036
access: public
definition: https://synbiohub.org/public/igem/BBa_R0040/1

BBa_I0462
type: Component
persistent identity: component2282668
display id: component2282668
version: 1
top level: BBa_K082036
access: public
definition: LuxR

BBa_R0040
type: Component
persistent identity: component2282649
display id: component2282649
version: 1
top level: BBa_K082036
access: public
definition: https://synbiohub.org/public/igem/BBa_R0040/1

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2282649
display id: annotation2282649
version: 1
top level: BBa_K082036
location: https://synbiohub.org/public/igem/BBa_K082036/annotation2282649/range2282649/1
component: BBa_R0040

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type: Range
persistent identity: range2282649
display id: range2282649
version: 1
direction: direction/forward
top level: BBa_K082036
start: 1
end: 54
orientation: inline

BBa_C0070
type: SequenceAnnotation
persistent identity: annotation2282679
display id: annotation2282679
version: 1
top level: BBa_K082036
location: https://synbiohub.org/public/igem/BBa_K082036/annotation2282679/range2282679/1
component: BBa_C0070

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type: Range
persistent identity: range2282679
display id: range2282679
version: 1
direction: direction/reverse
top level: BBa_K082036
start: 1087
end: 1753
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2282686
display id: annotation2282686
version: 1
top level: BBa_K082036
location: https://synbiohub.org/public/igem/BBa_K082036/annotation2282686/range2282686/1
component: BBa_B0015

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type: Range
persistent identity: range2282686
display id: range2282686
version: 1
direction: direction/reverse
top level: BBa_K082036
start: 1762
end: 1890
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2282675
display id: annotation2282675
version: 1
top level: BBa_K082036
location: https://synbiohub.org/public/igem/BBa_K082036/annotation2282675/range2282675/1
component: BBa_B0034

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type: Range
persistent identity: range2282675
display id: range2282675
version: 1
direction: direction/reverse
top level: BBa_K082036
start: 1069
end: 1080
orientation: inline

BBa_I0462
type: SequenceAnnotation
persistent identity: annotation2282668
display id: annotation2282668
version: 1
top level: BBa_K082036
location: https://synbiohub.org/public/igem/BBa_K082036/annotation2282668/range2282668/1
component: BBa_I0462

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type: Range
persistent identity: range2282668
display id: range2282668
version: 1
direction: direction/reverse
top level: BBa_K082036
start: 63
end: 998
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2282669
display id: annotation2282669
version: 1
top level: BBa_K082036
location: https://synbiohub.org/public/igem/BBa_K082036/annotation2282669/range2282669/1
component: BBa_R0040

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type: Range
persistent identity: range2282669
display id: range2282669
version: 1
direction: direction/forward
top level: BBa_K082036
start: 1007
end: 1060
orientation: inline

BBa_K546001
type: ComponentDefinition
persistent identity: BBa K546001
display id: BBa_K546001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Lux pL controlled luxR with lux pR controlled AHL degrading enzyme AiiA(lva tag).
creator: M. Hesselman
created: 2011-09-13T11:00:00Z
modified: 2015-05-08T01:12:39Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _713_
m user id: 0
owner id: 9055
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: The F2621 BioBrick device contains a lux pL regulated luxR repressor/activator and a lux pR promoter that is linked to an 3OC6HSL, an AHL autoinducer molecule, synthetase. The lux pL promoter yields a weak constitutive downstream expression. When LuxR and 3OC6HSL are produced, the lux pL promoter, however, will be repressed by a complex of the two. If 3OC6HSL is present in the cell, the autoinducer inactivation enzyme A (''aiiA''), with an LVA Tag, will inactivate the 3OC6HSL autoinducer molecules.
mutable notes: The original R0063 BioBrick part (promoter) of the F2621 BioBrick device turned out to be absent in our construct, as was shown by a sequence analysis. To increase the transcription activation there has been put an extra R0063 BioBrick part promoter in front of the F2621 BioBrick device.
mutable provenance: The lux p promoters originate from the quorum sensing ability of ''Vibrio fischeri''.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_I0460
component: BBa_F2621
sequence annotation: BBa_F2621
sequence annotation: BBa_I0460
sequence: https://synbiohub.org/public/igem/BBa_K546001_sequence/1

BBa_I0460
type: Component
persistent identity: component2128248
display id: component2128248
version: 1
top level: BBa_K546001
access: public
definition: BBa_I0460

BBa_F2621
type: Component
persistent identity: component2128233
display id: component2128233
version: 1
top level: BBa_K546001
access: public
definition: BBa_F2621

BBa_F2621
type: SequenceAnnotation
persistent identity: annotation2128233
display id: annotation2128233
version: 1
top level: BBa_K546001
location: https://synbiohub.org/public/igem/BBa_K546001/annotation2128233/range2128233/1
component: BBa_F2621

https://synbiohub.org/public/igem/BBa_K546001/annotation2128233/range2128233/1
type: Range
persistent identity: range2128233
display id: range2128233
version: 1
direction: direction/reverse
top level: BBa_K546001
start: 1
end: 1158
orientation: inline

BBa_I0460
type: SequenceAnnotation
persistent identity: annotation2128248
display id: annotation2128248
version: 1
top level: BBa_K546001
location: https://synbiohub.org/public/igem/BBa_K546001/annotation2128248/range2128248/1
component: BBa_I0460

https://synbiohub.org/public/igem/BBa_K546001/annotation2128248/range2128248/1
type: Range
persistent identity: range2128248
display id: range2128248
version: 1
direction: direction/reverse
top level: BBa_K546001
start: 1167
end: 2135
orientation: inline

BBa_K574001
type: ComponentDefinition
persistent identity: BBa K574001
display id: BBa_K574001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TetR regulated by 3OC6HSL
creator: Lei Wei
created: 2011-09-28T11:00:00Z
modified: 2015-05-08T01:12:44Z
discontinued: false
dominant: false
experience: experience/None
group u list: _745_
m user id: 0
owner id: 8529
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: luxI Generator + p(luxR) +tetR.
mutable notes: Empty.
mutable provenance: Existing Parts form PartRegistry.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Signalling
role: SO:0000804
component: BBa_K574000
component: BBa_K145270
sequence annotation: BBa_K574000
sequence annotation: BBa_K145270
sequence: https://synbiohub.org/public/igem/BBa_K574001_sequence/1

BBa_K574000
type: Component
persistent identity: component2289946
display id: component2289946
version: 1
top level: BBa_K574001
access: public
definition: https://synbiohub.org/public/igem/BBa_K574000/1

BBa_K145270
type: Component
persistent identity: component2289927
display id: component2289927
version: 1
top level: BBa_K574001
access: public
definition: https://synbiohub.org/public/igem/BBa_K145270/1

BBa_K574000
type: SequenceAnnotation
persistent identity: annotation2289946
display id: annotation2289946
version: 1
top level: BBa_K574001
location: https://synbiohub.org/public/igem/BBa_K574001/annotation2289946/range2289946/1
component: BBa_K574000

https://synbiohub.org/public/igem/BBa_K574001/annotation2289946/range2289946/1
type: Range
persistent identity: range2289946
display id: range2289946
version: 1
direction: direction/reverse
top level: BBa_K574001
start: 988
end: 1890
orientation: inline

BBa_K145270
type: SequenceAnnotation
persistent identity: annotation2289927
display id: annotation2289927
version: 1
top level: BBa_K574001
location: https://synbiohub.org/public/igem/BBa_K574001/annotation2289927/range2289927/1
component: BBa_K145270

https://synbiohub.org/public/igem/BBa_K574001/annotation2289927/range2289927/1
type: Range
persistent identity: range2289927
display id: range2289927
version: 1
direction: direction/reverse
top level: BBa_K574001
start: 1
end: 979
orientation: inline

rhII
type: ComponentDefinition
persistent identity: BBa C0170
display id: BBa_C0170
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: autoinducer synthetase for N-butyryl-HSL (BHL) and HHL (no LVA)
creator: jcbraff
created: 2004-05-26T11:00:00Z
modified: 2015-08-31T04:07:24Z
discontinued: false
dominant: true
experience: experience/None
group u list: _11_1_
m user id: 0
owner id: 61
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: same as C0070 except no LVA tag
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: rhII
sequence: https://synbiohub.org/public/igem/BBa_C0170_sequence/1

rhII
type: SequenceAnnotation
persistent identity: annotation1891586
display id: annotation1891586
version: 1
top level: rhII
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_C0170/annotation1891586/range1891586/1

https://synbiohub.org/public/igem/BBa_C0170/annotation1891586/range1891586/1
type: Range
persistent identity: range1891586
display id: range1891586
version: 1
direction: direction/forward
top level: rhII
start: 1
end: 609
orientation: inline

BBa_K091158
type: ComponentDefinition
persistent identity: BBa K091158
display id: BBa_K091158
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS+lsrK
creator: Xiao Zhu
created: 2008-07-27T11:00:00Z
modified: 2015-05-08T01:08:38Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _191_
m user id: 0
owner id: 3130
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: RBS here is B0034.
mutable notes: no
mutable provenance: lsrK is from E. coli MG1655
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_K091002
component: BBa_B0034
sequence annotation: BBa_B0034
sequence annotation: BBa_K091002
sequence: https://synbiohub.org/public/igem/BBa_K091158_sequence/1

BBa_K091002
type: Component
persistent identity: component1968753
display id: component1968753
version: 1
top level: BBa_K091158
access: public
definition: https://synbiohub.org/public/igem/BBa_K091002/1

BBa_B0034
type: Component
persistent identity: component1968752
display id: component1968752
version: 1
top level: BBa_K091158
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1968752
display id: annotation1968752
version: 1
top level: BBa_K091158
location: https://synbiohub.org/public/igem/BBa_K091158/annotation1968752/range1968752/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K091158/annotation1968752/range1968752/1
type: Range
persistent identity: range1968752
display id: range1968752
version: 1
direction: direction/reverse
top level: BBa_K091158
start: 1
end: 12
orientation: inline

BBa_K091002
type: SequenceAnnotation
persistent identity: annotation1968753
display id: annotation1968753
version: 1
top level: BBa_K091158
location: https://synbiohub.org/public/igem/BBa_K091158/annotation1968753/range1968753/1
component: BBa_K091002

https://synbiohub.org/public/igem/BBa_K091158/annotation1968753/range1968753/1
type: Range
persistent identity: range1968753
display id: range1968753
version: 1
direction: direction/reverse
top level: BBa_K091158
start: 19
end: 1611
orientation: inline

BBa_K332031
type: ComponentDefinition
persistent identity: BBa K332031
display id: BBa_K332031
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Constitutive promoter + 37℃ induced RBS + tetR + double terminator
creator: KUO, CHIA-CHUN
created: 2010-10-21T11:00:00Z
modified: 2015-05-08T01:12:05Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _452_
m user id: 0
owner id: 7059
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: we took advantage of the interaction between 37?? C induced RBS, tetR gene and pTet promoter to create the temperature regulating circuit. The following is a description of our prescribed strategy. First, we use the constitutive promoter, 37?? C induced RBS (K115002), the tetR gene and terminators. We will call this part the ???Regulator???. We speculate that when the ???Regulator??? and ???Production??? sections are combined, we can achieve a high bacteria growth rate and close to no target protein producing rate at T>37??C, and low bacteria growth rate and high target protein producing rate at T<37??C.
mutable notes: To regulate following gene by temperature
mutable provenance: iGEM08_TUDelft
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_C0040
component: BBa_J23101
component: BBa_K115002
component: BBa_J61048
sequence annotation: BBa_C0040
sequence annotation: BBa_J61048
sequence annotation: BBa_J23101
sequence annotation: BBa_K115002
sequence: https://synbiohub.org/public/igem/BBa_K332031_sequence/1

BBa_C0040
type: Component
persistent identity: component2250406
display id: component2250406
version: 1
top level: BBa_K332031
access: public
definition: https://synbiohub.org/public/igem/BBa_C0040/1

BBa_J23101
type: Component
persistent identity: component2250397
display id: component2250397
version: 1
top level: BBa_K332031
access: public
definition: https://synbiohub.org/public/igem/BBa_J23101/1

BBa_K115002
type: Component
persistent identity: component2250402
display id: component2250402
version: 1
top level: BBa_K332031
access: public
definition: https://synbiohub.org/public/igem/BBa_K115002/1

BBa_J61048
type: Component
persistent identity: component2250407
display id: component2250407
version: 1
top level: BBa_K332031
access: public
definition: https://synbiohub.org/public/igem/BBa_J61048/1

BBa_C0040
type: SequenceAnnotation
persistent identity: annotation2250406
display id: annotation2250406
version: 1
top level: BBa_K332031
location: https://synbiohub.org/public/igem/BBa_K332031/annotation2250406/range2250406/1
component: BBa_C0040

https://synbiohub.org/public/igem/BBa_K332031/annotation2250406/range2250406/1
type: Range
persistent identity: range2250406
display id: range2250406
version: 1
direction: direction/reverse
top level: BBa_K332031
start: 102
end: 786
orientation: inline

BBa_J61048
type: SequenceAnnotation
persistent identity: annotation2250407
display id: annotation2250407
version: 1
top level: BBa_K332031
location: https://synbiohub.org/public/igem/BBa_K332031/annotation2250407/range2250407/1
component: BBa_J61048

https://synbiohub.org/public/igem/BBa_K332031/annotation2250407/range2250407/1
type: Range
persistent identity: range2250407
display id: range2250407
version: 1
direction: direction/reverse
top level: BBa_K332031
start: 795
end: 907
orientation: inline

BBa_J23101
type: SequenceAnnotation
persistent identity: annotation2250397
display id: annotation2250397
version: 1
top level: BBa_K332031
location: https://synbiohub.org/public/igem/BBa_K332031/annotation2250397/range2250397/1
component: BBa_J23101

https://synbiohub.org/public/igem/BBa_K332031/annotation2250397/range2250397/1
type: Range
persistent identity: range2250397
display id: range2250397
version: 1
direction: direction/reverse
top level: BBa_K332031
start: 1
end: 35
orientation: inline

BBa_K115002
type: SequenceAnnotation
persistent identity: annotation2250402
display id: annotation2250402
version: 1
top level: BBa_K332031
location: https://synbiohub.org/public/igem/BBa_K332031/annotation2250402/range2250402/1
component: BBa_K115002

https://synbiohub.org/public/igem/BBa_K332031/annotation2250402/range2250402/1
type: Range
persistent identity: range2250402
display id: range2250402
version: 1
direction: direction/reverse
top level: BBa_K332031
start: 44
end: 95
orientation: inline

luxI
type: ComponentDefinition
persistent identity: BBa C0061
display id: BBa_C0061
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: autoinducer synthetase for AHL
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:23Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Synthesizes 3OC6HSL, which binds to LuxR. The lux cassette of V. fischeri contains a left and a right promoter. The right promoter gives weak constitutive expression of downstream genes.This expression is up-regulated by the action of the Lux repressor, LuxR. Two molecules of LuxR protein form a complex with two molecules the signalling compound HSL. This complex binds to a palindromic site on the promoter, increasing the rate of transcription.
mutable notes: An LVA tail (sequence: AANDENYALVA) was added to increase protein degradation. .
mutable provenance: V. fischeri AF170104
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: BBa_C0061
sequence annotation: Help:Barcodes
sequence annotation: LVA
sequence annotation: luxI
sequence: https://synbiohub.org/public/igem/BBa_C0061_sequence/1

BBa_C0061
type: SequenceAnnotation
persistent identity: annotation7038
display id: annotation7038
version: 1
top level: luxI
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_C0061/annotation7038/range7038/1

https://synbiohub.org/public/igem/BBa_C0061/annotation7038/range7038/1
type: Range
persistent identity: range7038
display id: range7038
version: 1
direction: direction/forward
top level: luxI
start: 1
end: 618
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213985
display id: annotation2213985
version: 1
top level: luxI
role: SO:0000807
role: feature/barcode
location: https://synbiohub.org/public/igem/BBa_C0061/annotation2213985/range2213985/1

https://synbiohub.org/public/igem/BBa_C0061/annotation2213985/range2213985/1
type: Range
persistent identity: range2213985
display id: range2213985
version: 1
direction: direction/forward
top level: luxI
start: 619
end: 643
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation1760
display id: annotation1760
version: 1
top level: luxI
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_C0061/annotation1760/range1760/1

https://synbiohub.org/public/igem/BBa_C0061/annotation1760/range1760/1
type: Range
persistent identity: range1760
display id: range1760
version: 1
direction: direction/forward
top level: luxI
start: 580
end: 611
orientation: inline

luxI
type: SequenceAnnotation
persistent identity: annotation1761
display id: annotation1761
version: 1
top level: luxI
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_C0061/annotation1761/range1761/1

https://synbiohub.org/public/igem/BBa_C0061/annotation1761/range1761/1
type: Range
persistent identity: range1761
display id: range1761
version: 1
direction: direction/forward
top level: luxI
start: 1
end: 579
orientation: inline

BBa_J07043
type: ComponentDefinition
persistent identity: BBa J07043
display id: BBa_J07043
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: J07012.B0015 (scFv 4-4-20.TT)
creator: Jenny Nguyen
created: 2005-08-02T11:00:00Z
modified: 2015-08-31T04:08:20Z
discontinued: false
dominant: false
group u list: _21_
m user id: 0
owner id: 350
owning group id: 21
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Anti-fluorescein scFv (4-4-20, no mutations) with transcriptional terminator.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Signalling
role: SO:0000804
component: BBa_J07012
component: BBa_B0010
component: BBa_B0012
sequence annotation: BBa_J07012
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_J07043_sequence/1

BBa_J07012
type: Component
persistent identity: component1651124
display id: component1651124
version: 1
top level: BBa_J07043
access: public
definition: BBa_J07012

BBa_B0010
type: Component
persistent identity: component1651129
display id: component1651129
version: 1
top level: BBa_J07043
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
type: Component
persistent identity: component1651139
display id: component1651139
version: 1
top level: BBa_J07043
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_J07012
type: SequenceAnnotation
persistent identity: annotation1651124
display id: annotation1651124
version: 1
top level: BBa_J07043
location: https://synbiohub.org/public/igem/BBa_J07043/annotation1651124/range1651124/1
component: BBa_J07012

https://synbiohub.org/public/igem/BBa_J07043/annotation1651124/range1651124/1
type: Range
persistent identity: range1651124
display id: range1651124
version: 1
direction: direction/forward
top level: BBa_J07043
start: 1
end: 762
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1651129
display id: annotation1651129
version: 1
top level: BBa_J07043
location: https://synbiohub.org/public/igem/BBa_J07043/annotation1651129/range1651129/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_J07043/annotation1651129/range1651129/1
type: Range
persistent identity: range1651129
display id: range1651129
version: 1
direction: direction/forward
top level: BBa_J07043
start: 771
end: 850
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1651139
display id: annotation1651139
version: 1
top level: BBa_J07043
location: https://synbiohub.org/public/igem/BBa_J07043/annotation1651139/range1651139/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_J07043/annotation1651139/range1651139/1
type: Range
persistent identity: range1651139
display id: range1651139
version: 1
direction: direction/forward
top level: BBa_J07043
start: 859
end: 899
orientation: inline

LacIM
type: ComponentDefinition
persistent identity: BBa K819012
display id: BBa_K819012
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Luminesensor repressible LacIM generator
creator: ZHANG Hong
created: 2012-09-02T11:00:00Z
modified: 2015-05-08T01:13:29Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1077_
m user id: 0
owner id: 13785
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: LacI mutant driven by luminesensor repressible SulA promoter in 408 form.
mutable notes: B0034, CDS of LacI mutant and B0015 were connected directly to SulA promoter in 408 form.
mutable provenance: Sequene of the LacI mutant is from Partregistry.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: SulA408 Promoter
sequence annotation: LacIM(K395400)
sequence: https://synbiohub.org/public/igem/BBa_K819012_sequence/1

SulA408 Promoter
type: SequenceAnnotation
persistent identity: annotation2181628
display id: annotation2181628
version: 1
top level: LacIM
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K819012/annotation2181628/range2181628/1

https://synbiohub.org/public/igem/BBa_K819012/annotation2181628/range2181628/1
type: Range
persistent identity: range2181628
display id: range2181628
version: 1
direction: direction/forward
top level: LacIM
start: 1
end: 64
orientation: inline

LacIM(K395400)
type: SequenceAnnotation
persistent identity: annotation2181627
display id: annotation2181627
version: 1
top level: LacIM
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K819012/annotation2181627/range2181627/1

https://synbiohub.org/public/igem/BBa_K819012/annotation2181627/range2181627/1
type: Range
persistent identity: range2181627
display id: range2181627
version: 1
direction: direction/forward
top level: LacIM
start: 65
end: 1292
orientation: inline

BBa_I13220
type: ComponentDefinition
persistent identity: BBa I13220
display id: BBa_I13220
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Natural Lux Promoter 2nd Generation
creator: mit
created: 2004-08-17T11:00:00Z
modified: 2015-08-31T04:07:33Z
discontinued: false
dominant: false
experience: experience/None
group u list: _6_
m user id: 0
owner id: 101
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: A slightly different sequence than the original; the coverage has been shifted. It should function the same way the original was intended, left repression by hsl-bound LuxR and right activation by hsl-bound LuxR.
mutable provenance: pTK-1
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Signalling
role: SO:0000804
sequence: https://synbiohub.org/public/igem/BBa_I13220_sequence/1

lasI
type: ComponentDefinition
persistent identity: BBa C0178
display id: BBa_C0178
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: autoinducer synthetase for PAI from Pseudomonas aeruginosa (no LVA)
creator: jcbraff
created: 2004-05-26T11:00:00Z
modified: 2015-08-31T04:07:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_1_
m user id: 0
owner id: 61
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: same as C0078 except no LVA tag
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: lasI
sequence: https://synbiohub.org/public/igem/BBa_C0178_sequence/1

lasI
type: SequenceAnnotation
persistent identity: annotation1937124
display id: annotation1937124
version: 1
top level: lasI
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_C0178/annotation1937124/range1937124/1

https://synbiohub.org/public/igem/BBa_C0178/annotation1937124/range1937124/1
type: Range
persistent identity: range1937124
display id: range1937124
version: 1
direction: direction/forward
top level: lasI
start: 1
end: 609
orientation: inline

BBa_K546003
type: ComponentDefinition
persistent identity: BBa K546003
display id: BBa_K546003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Lux pL controlled LuxR + lux pR promoter.
creator: Brendan Ryback, Matthijn Hesselman
created: 2011-09-13T11:00:00Z
modified: 2015-05-08T01:12:39Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _713_
m user id: 0
owner id: 8616
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This part was made as a replacement for a defective version of BBa_F2621 which lacked the R0063 promoter, but was otherwise intact and functional. It shows a significantly higher response to 3OC6HSL than the incomplete BBa_F2621 (which is present in the Registry as BBa_K317037)
mutable notes: No specific considerations.
mutable provenance: Was constructed by cloning BBa_R0063 in front of the incomplete BBa_F2621 (sent from the Registry in July 2011 lacking the R0063 subcomponent)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Signalling
role: SO:0000804
component: BBa_K317037
component: BBa_R0063
sequence annotation: BBa_K317037
sequence annotation: BBa_R0063
sequence: https://synbiohub.org/public/igem/BBa_K546003_sequence/1

BBa_K317037
type: Component
persistent identity: component2264075
display id: component2264075
version: 1
top level: BBa_K546003
access: public
definition: https://synbiohub.org/public/igem/BBa_K317037/1

BBa_R0063
type: Component
persistent identity: component2264050
display id: component2264050
version: 1
top level: BBa_K546003
access: public
definition: https://synbiohub.org/public/igem/BBa_R0063/1

BBa_K317037
type: SequenceAnnotation
persistent identity: annotation2264075
display id: annotation2264075
version: 1
top level: BBa_K546003
location: https://synbiohub.org/public/igem/BBa_K546003/annotation2264075/range2264075/1
component: BBa_K317037

https://synbiohub.org/public/igem/BBa_K546003/annotation2264075/range2264075/1
type: Range
persistent identity: range2264075
display id: range2264075
version: 1
direction: direction/reverse
top level: BBa_K546003
start: 160
end: 1158
orientation: inline

BBa_R0063
type: SequenceAnnotation
persistent identity: annotation2264050
display id: annotation2264050
version: 1
top level: BBa_K546003
location: https://synbiohub.org/public/igem/BBa_K546003/annotation2264050/range2264050/1
component: BBa_R0063

https://synbiohub.org/public/igem/BBa_K546003/annotation2264050/range2264050/1
type: Range
persistent identity: range2264050
display id: range2264050
version: 1
direction: direction/forward
top level: BBa_K546003
start: 1
end: 151
orientation: inline

BBa_J13040
type: ComponentDefinition
persistent identity: BBa J13040
display id: BBa_J13040
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pOmpR dependent 3OC6HSL sender device
creator: Jeff Tabor
created: 2005-07-18T11:00:00Z
modified: 2015-08-31T04:08:29Z
discontinued: false
dominant: false
experience: experience/None
group u list: _37_
m user id: 0
owner id: 88
owning group id: 37
sample status: In stock
status: status/Available
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Signalling
role: SO:0000804
component: BBa_B0010
component: BBa_B0034
component: BBa_B0012
component: BBa_R0082
component: BBa_C0061
sequence annotation: BBa_B0034
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_R0082
sequence annotation: BBa_C0061
sequence: https://synbiohub.org/public/igem/BBa_J13040_sequence/1

BBa_B0010
type: Component
persistent identity: component1587156
display id: component1587156
version: 1
top level: BBa_J13040
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0034
type: Component
persistent identity: component1587139
display id: component1587139
version: 1
top level: BBa_J13040
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0012
type: Component
persistent identity: component1587166
display id: component1587166
version: 1
top level: BBa_J13040
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_R0082
type: Component
persistent identity: component1587134
display id: component1587134
version: 1
top level: BBa_J13040
access: public
definition: https://synbiohub.org/public/igem/BBa_R0082/1

BBa_C0061
type: Component
persistent identity: component1587149
display id: component1587149
version: 1
top level: BBa_J13040
access: public
definition: luxI

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1587139
display id: annotation1587139
version: 1
top level: BBa_J13040
location: https://synbiohub.org/public/igem/BBa_J13040/annotation1587139/range1587139/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_J13040/annotation1587139/range1587139/1
type: Range
persistent identity: range1587139
display id: range1587139
version: 1
direction: direction/forward
top level: BBa_J13040
start: 117
end: 128
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1587156
display id: annotation1587156
version: 1
top level: BBa_J13040
location: https://synbiohub.org/public/igem/BBa_J13040/annotation1587156/range1587156/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_J13040/annotation1587156/range1587156/1
type: Range
persistent identity: range1587156
display id: range1587156
version: 1
direction: direction/forward
top level: BBa_J13040
start: 786
end: 865
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1587166
display id: annotation1587166
version: 1
top level: BBa_J13040
location: https://synbiohub.org/public/igem/BBa_J13040/annotation1587166/range1587166/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_J13040/annotation1587166/range1587166/1
type: Range
persistent identity: range1587166
display id: range1587166
version: 1
direction: direction/forward
top level: BBa_J13040
start: 874
end: 914
orientation: inline

BBa_R0082
type: SequenceAnnotation
persistent identity: annotation1587134
display id: annotation1587134
version: 1
top level: BBa_J13040
location: https://synbiohub.org/public/igem/BBa_J13040/annotation1587134/range1587134/1
component: BBa_R0082

https://synbiohub.org/public/igem/BBa_J13040/annotation1587134/range1587134/1
type: Range
persistent identity: range1587134
display id: range1587134
version: 1
direction: direction/forward
top level: BBa_J13040
start: 1
end: 108
orientation: inline

BBa_C0061
type: SequenceAnnotation
persistent identity: annotation1587149
display id: annotation1587149
version: 1
top level: BBa_J13040
location: https://synbiohub.org/public/igem/BBa_J13040/annotation1587149/range1587149/1
component: BBa_C0061

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type: Range
persistent identity: range1587149
display id: range1587149
version: 1
direction: direction/forward
top level: BBa_J13040
start: 135
end: 752
orientation: inline

BBa_I726061
type: ComponentDefinition
persistent identity: BBa I726061
display id: BBa_I726061
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Trc-LRY
creator: Bangalore iGEM
created: 2007-07-03T11:00:00Z
modified: 2015-08-31T04:07:55Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _107_
m user id: 0
owner id: 2077
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Abcdefghijkl..
mutable notes: none
mutable provenance: Registry
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_B0034
component: BBa_E0030
component: BBa_B0010
component: BBa_B0012
component: BBa_C0062
component: BBa_R0011
sequence annotation: BBa_R0011
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_E0030
sequence annotation: BBa_C0062
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_I726061_sequence/1

BBa_B0034
type: Component
persistent identity: component2265594
display id: component2265594
version: 1
top level: BBa_I726061
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_E0030
type: Component
persistent identity: component2265603
display id: component2265603
version: 1
top level: BBa_I726061
access: public
definition: https://synbiohub.org/public/igem/BBa_E0030/1

BBa_B0010
type: Component
persistent identity: component2265604
display id: component2265604
version: 1
top level: BBa_I726061
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0034
type: Component
persistent identity: component2265602
display id: component2265602
version: 1
top level: BBa_I726061
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0012
type: Component
persistent identity: component2265606
display id: component2265606
version: 1
top level: BBa_I726061
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_C0062
type: Component
persistent identity: component2265597
display id: component2265597
version: 1
top level: BBa_I726061
access: public
definition: luxr

BBa_R0011
type: Component
persistent identity: component2265588
display id: component2265588
version: 1
top level: BBa_I726061
access: public
definition: https://synbiohub.org/public/igem/BBa_R0011/1

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation2265588
display id: annotation2265588
version: 1
top level: BBa_I726061
location: https://synbiohub.org/public/igem/BBa_I726061/annotation2265588/range2265588/1
component: BBa_R0011

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type: Range
persistent identity: range2265588
display id: range2265588
version: 1
direction: direction/forward
top level: BBa_I726061
start: 1
end: 54
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2265604
display id: annotation2265604
version: 1
top level: BBa_I726061
location: https://synbiohub.org/public/igem/BBa_I726061/annotation2265604/range2265604/1
component: BBa_B0010

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type: Range
persistent identity: range2265604
display id: range2265604
version: 1
direction: direction/forward
top level: BBa_I726061
start: 1620
end: 1699
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2265606
display id: annotation2265606
version: 1
top level: BBa_I726061
location: https://synbiohub.org/public/igem/BBa_I726061/annotation2265606/range2265606/1
component: BBa_B0012

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type: Range
persistent identity: range2265606
display id: range2265606
version: 1
direction: direction/forward
top level: BBa_I726061
start: 1708
end: 1748
orientation: inline

BBa_E0030
type: SequenceAnnotation
persistent identity: annotation2265603
display id: annotation2265603
version: 1
top level: BBa_I726061
location: https://synbiohub.org/public/igem/BBa_I726061/annotation2265603/range2265603/1
component: BBa_E0030

https://synbiohub.org/public/igem/BBa_I726061/annotation2265603/range2265603/1
type: Range
persistent identity: range2265603
display id: range2265603
version: 1
direction: direction/reverse
top level: BBa_I726061
start: 889
end: 1611
orientation: inline

BBa_C0062
type: SequenceAnnotation
persistent identity: annotation2265597
display id: annotation2265597
version: 1
top level: BBa_I726061
location: https://synbiohub.org/public/igem/BBa_I726061/annotation2265597/range2265597/1
component: BBa_C0062

https://synbiohub.org/public/igem/BBa_I726061/annotation2265597/range2265597/1
type: Range
persistent identity: range2265597
display id: range2265597
version: 1
direction: direction/forward
top level: BBa_I726061
start: 82
end: 837
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2265594
display id: annotation2265594
version: 1
top level: BBa_I726061
location: https://synbiohub.org/public/igem/BBa_I726061/annotation2265594/range2265594/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I726061/annotation2265594/range2265594/1
type: Range
persistent identity: range2265594
display id: range2265594
version: 1
direction: direction/reverse
top level: BBa_I726061
start: 64
end: 75
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2265602
display id: annotation2265602
version: 1
top level: BBa_I726061
location: https://synbiohub.org/public/igem/BBa_I726061/annotation2265602/range2265602/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I726061/annotation2265602/range2265602/1
type: Range
persistent identity: range2265602
display id: range2265602
version: 1
direction: direction/reverse
top level: BBa_I726061
start: 871
end: 882
orientation: inline

BBa_K091139
type: ComponentDefinition
persistent identity: BBa K091139
display id: BBa_K091139
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS+lsrR+TT
creator: Xiao Zhu
created: 2008-07-14T11:00:00Z
modified: 2015-05-08T01:08:38Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _191_
m user id: 0
owner id: 3130
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: K091138 with double terminator B0015.
mutable notes: no
mutable provenance: lsrR comes from E. coli MG1655
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0012
component: BBa_B0010
component: BBa_K091001
component: BBa_B0034
sequence annotation: BBa_B0034
sequence annotation: BBa_K091001
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_K091139_sequence/1

BBa_B0012
type: Component
persistent identity: component1967019
display id: component1967019
version: 1
top level: BBa_K091139
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0010
type: Component
persistent identity: component1967017
display id: component1967017
version: 1
top level: BBa_K091139
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_K091001
type: Component
persistent identity: component1967016
display id: component1967016
version: 1
top level: BBa_K091139
access: public
definition: https://synbiohub.org/public/igem/BBa_K091001/1

BBa_B0034
type: Component
persistent identity: component1967015
display id: component1967015
version: 1
top level: BBa_K091139
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1967015
display id: annotation1967015
version: 1
top level: BBa_K091139
location: https://synbiohub.org/public/igem/BBa_K091139/annotation1967015/range1967015/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K091139/annotation1967015/range1967015/1
type: Range
persistent identity: range1967015
display id: range1967015
version: 1
direction: direction/reverse
top level: BBa_K091139
start: 1
end: 12
orientation: inline

BBa_K091001
type: SequenceAnnotation
persistent identity: annotation1967016
display id: annotation1967016
version: 1
top level: BBa_K091139
location: https://synbiohub.org/public/igem/BBa_K091139/annotation1967016/range1967016/1
component: BBa_K091001

https://synbiohub.org/public/igem/BBa_K091139/annotation1967016/range1967016/1
type: Range
persistent identity: range1967016
display id: range1967016
version: 1
direction: direction/reverse
top level: BBa_K091139
start: 19
end: 972
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1967017
display id: annotation1967017
version: 1
top level: BBa_K091139
location: https://synbiohub.org/public/igem/BBa_K091139/annotation1967017/range1967017/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K091139/annotation1967017/range1967017/1
type: Range
persistent identity: range1967017
display id: range1967017
version: 1
direction: direction/forward
top level: BBa_K091139
start: 981
end: 1060
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1967019
display id: annotation1967019
version: 1
top level: BBa_K091139
location: https://synbiohub.org/public/igem/BBa_K091139/annotation1967019/range1967019/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K091139/annotation1967019/range1967019/1
type: Range
persistent identity: range1967019
display id: range1967019
version: 1
direction: direction/forward
top level: BBa_K091139
start: 1069
end: 1109
orientation: inline

BBa_K1687001
type: ComponentDefinition
persistent identity: BBa K1687001
display id: BBa_K1687001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: An enhanced lacI protein generator, inhibits Plac perfectly
creator: 2015 SZMS iGEM, Yineng Zhou
created: 2015-09-15T11:00:00Z
modified: 2015-09-17T11:44:27Z
discontinued: true
dominant: false
experience: experience/None
group u list: _2105_
m user id: 24745
owner id: 24745
owning group id: 9
status: status/Deleted
bookmark: false
mutable description: This is an enhanced lacI generator. Induced point mutations shifted the structure so it can stop transmitting more perfectly.
mutable notes: Several point mutations were made while this works the best.
mutable provenance: A graduate essay published in Zhejiang University, China. Sequence is open to the public.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Enhanced LacI generator
sequence: https://synbiohub.org/public/igem/BBa_K1687001_sequence/1

Enhanced LacI generator
type: SequenceAnnotation
persistent identity: annotation2463575
display id: annotation2463575
version: 1
top level: BBa_K1687001
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1687001/annotation2463575/range2463575/1

https://synbiohub.org/public/igem/BBa_K1687001/annotation2463575/range2463575/1
type: Range
persistent identity: range2463575
display id: range2463575
version: 1
direction: direction/forward
top level: BBa_K1687001
start: 1
end: 1083
orientation: inline