//function/biosynthesis/odorant
type: Collection
persistent identity: function biosynthesis odorant
display id: function_biosynthesis_odorant
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
owned by: james
owned by: myers
member: BAT2
member: BBa_J45098
member: ATF1
member: BBa_J45298
member: COMT
member: BBa_K1660003
member: SAMT
member: BAMT
member: pchBA
member: BSMT1

pchBA
type: ComponentDefinition
persistent identity: BBa J45017
display id: BBa_J45017
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: isochorismate pyruvate-lyase and isochorismate synthase (pchBA); converts chorismate to salicylate
creator: MIT IGEM 2006
created: 2006-08-20T11:00:00Z
modified: 2015-08-31T04:08:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _84_
m user id: 0
owner id: 1147
owning group id: 84
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: pchBA codes for proteins used to generate salicylic acid. It doesn't make a fusion protein, even though the coding regions overlap; the RBS for pchA is located at the end of the coding region of pchB.
mutable notes: n/a
mutable provenance: pchBA is found in pseudomonas. The plasmid was kindly sent to the MIT iGEM team by Dr. Cornelia Riemmann from the Universite de Lausanne.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: pchA double TAA stop codon
sequence annotation: pchA start codon
sequence annotation: PchA
sequence annotation: pchB stop codon
sequence annotation: PchB
sequence annotation: pchB start codon
sequence annotation: g -> a to eliminate PstI site
sequence: https://synbiohub.org/public/igem/BBa_J45017_sequence/1

pchA double TAA stop codon
type: SequenceAnnotation
persistent identity: annotation1897457
display id: annotation1897457
version: 1
top level: pchBA
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_J45017/annotation1897457/range1897457/1

https://synbiohub.org/public/igem/BBa_J45017/annotation1897457/range1897457/1
type: Range
persistent identity: range1897457
display id: range1897457
version: 1
direction: direction/forward
top level: pchBA
start: 1731
end: 1736
orientation: inline

pchA start codon
type: SequenceAnnotation
persistent identity: annotation1897454
display id: annotation1897454
version: 1
top level: pchBA
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_J45017/annotation1897454/range1897454/1

https://synbiohub.org/public/igem/BBa_J45017/annotation1897454/range1897454/1
type: Range
persistent identity: range1897454
display id: range1897454
version: 1
direction: direction/forward
top level: pchBA
start: 303
end: 305
orientation: inline

PchA
type: SequenceAnnotation
persistent identity: annotation1961297
display id: annotation1961297
version: 1
top level: pchBA
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J45017/annotation1961297/range1961297/1

https://synbiohub.org/public/igem/BBa_J45017/annotation1961297/range1961297/1
type: Range
persistent identity: range1961297
display id: range1961297
version: 1
direction: direction/forward
top level: pchBA
start: 303
end: 1736
orientation: inline

pchB stop codon
type: SequenceAnnotation
persistent identity: annotation1897456
display id: annotation1897456
version: 1
top level: pchBA
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_J45017/annotation1897456/range1897456/1

https://synbiohub.org/public/igem/BBa_J45017/annotation1897456/range1897456/1
type: Range
persistent identity: range1897456
display id: range1897456
version: 1
direction: direction/forward
top level: pchBA
start: 304
end: 306
orientation: inline

PchB
type: SequenceAnnotation
persistent identity: annotation1961296
display id: annotation1961296
version: 1
top level: pchBA
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J45017/annotation1961296/range1961296/1

https://synbiohub.org/public/igem/BBa_J45017/annotation1961296/range1961296/1
type: Range
persistent identity: range1961296
display id: range1961296
version: 1
direction: direction/forward
top level: pchBA
start: 1
end: 306
orientation: inline

pchB start codon
type: SequenceAnnotation
persistent identity: annotation1897455
display id: annotation1897455
version: 1
top level: pchBA
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_J45017/annotation1897455/range1897455/1

https://synbiohub.org/public/igem/BBa_J45017/annotation1897455/range1897455/1
type: Range
persistent identity: range1897455
display id: range1897455
version: 1
direction: direction/forward
top level: pchBA
start: 1
end: 3
orientation: inline

g -> a to eliminate PstI site
type: SequenceAnnotation
persistent identity: annotation1897453
display id: annotation1897453
version: 1
top level: pchBA
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_J45017/annotation1897453/range1897453/1

https://synbiohub.org/public/igem/BBa_J45017/annotation1897453/range1897453/1
type: Range
persistent identity: range1897453
display id: range1897453
version: 1
direction: direction/forward
top level: pchBA
start: 440
end: 440
orientation: inline

BAMT
type: ComponentDefinition
persistent identity: BBa J45002
display id: BBa_J45002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: SAM:benzoic acid carboxyl methyltransferase; converts benzoic acid to methyl benzoate (floral odor)
creator: Andr?? Green II
created: 2006-06-06T11:00:00Z
modified: 2015-08-31T04:08:49Z
discontinued: false
dominant: false
experience: experience/Fails
group u list: _84_
m user id: 0
owner id: 977
owning group id: 84
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: BAMT catalyzes the transfer of a methyl group from SAM (S-adenosyl methyltransferase) to benzoic acid, creating methyl benzoate. Methyl benzoate produces a "pleasant" smell.
mutable notes: none as of yet
mutable provenance: Genbank accession number(s): AF198492 This part is from A. majus.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: putative start codon 2
sequence annotation: double TAA stop codon
sequence annotation: A -> G to eliminate SpeI site
sequence annotation: BAMT
sequence annotation: putative start codon 1
sequence: https://synbiohub.org/public/igem/BBa_J45002_sequence/1

putative start codon 2
type: SequenceAnnotation
persistent identity: annotation1880476
display id: annotation1880476
version: 1
top level: BAMT
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_J45002/annotation1880476/range1880476/1

https://synbiohub.org/public/igem/BBa_J45002/annotation1880476/range1880476/1
type: Range
persistent identity: range1880476
display id: range1880476
version: 1
direction: direction/forward
top level: BAMT
start: 10
end: 12
orientation: inline

double TAA stop codon
type: SequenceAnnotation
persistent identity: annotation1880495
display id: annotation1880495
version: 1
top level: BAMT
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_J45002/annotation1880495/range1880495/1

https://synbiohub.org/public/igem/BBa_J45002/annotation1880495/range1880495/1
type: Range
persistent identity: range1880495
display id: range1880495
version: 1
direction: direction/forward
top level: BAMT
start: 1093
end: 1098
orientation: inline

A -> G to eliminate SpeI site
type: SequenceAnnotation
persistent identity: annotation1880506
display id: annotation1880506
version: 1
top level: BAMT
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_J45002/annotation1880506/range1880506/1

https://synbiohub.org/public/igem/BBa_J45002/annotation1880506/range1880506/1
type: Range
persistent identity: range1880506
display id: range1880506
version: 1
direction: direction/forward
top level: BAMT
start: 702
end: 702
orientation: inline

BAMT
type: SequenceAnnotation
persistent identity: annotation1880494
display id: annotation1880494
version: 1
top level: BAMT
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J45002/annotation1880494/range1880494/1

https://synbiohub.org/public/igem/BBa_J45002/annotation1880494/range1880494/1
type: Range
persistent identity: range1880494
display id: range1880494
version: 1
direction: direction/forward
top level: BAMT
start: 1
end: 1095
orientation: inline

putative start codon 1
type: SequenceAnnotation
persistent identity: annotation1880475
display id: annotation1880475
version: 1
top level: BAMT
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_J45002/annotation1880475/range1880475/1

https://synbiohub.org/public/igem/BBa_J45002/annotation1880475/range1880475/1
type: Range
persistent identity: range1880475
display id: range1880475
version: 1
direction: direction/forward
top level: BAMT
start: 1
end: 3
orientation: inline

SAMT
type: ComponentDefinition
persistent identity: BBa J45001
display id: BBa_J45001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: SAM:salicylic acid carboxyl methyltransferase; converts salicylic acid to methyl salicylate (winter
creator: Kate Broadbent
created: 2006-06-06T11:00:00Z
modified: 2015-08-31T04:08:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _84_
m user id: 0
owner id: 976
owning group id: 84
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: From snapdragon (A. Majus)
mutable notes: lala
mutable provenance: Dutareva
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: start codon
sequence annotation: SAMT
sequence annotation: double TAA stop codon
sequence: https://synbiohub.org/public/igem/BBa_J45001_sequence/1

start codon
type: SequenceAnnotation
persistent identity: annotation1880596
display id: annotation1880596
version: 1
top level: SAMT
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_J45001/annotation1880596/range1880596/1

https://synbiohub.org/public/igem/BBa_J45001/annotation1880596/range1880596/1
type: Range
persistent identity: range1880596
display id: range1880596
version: 1
direction: direction/forward
top level: SAMT
start: 1
end: 3
orientation: inline

SAMT
type: SequenceAnnotation
persistent identity: annotation1880595
display id: annotation1880595
version: 1
top level: SAMT
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J45001/annotation1880595/range1880595/1

https://synbiohub.org/public/igem/BBa_J45001/annotation1880595/range1880595/1
type: Range
persistent identity: range1880595
display id: range1880595
version: 1
direction: direction/forward
top level: SAMT
start: 1
end: 1151
orientation: inline

double TAA stop codon
type: SequenceAnnotation
persistent identity: annotation1880597
display id: annotation1880597
version: 1
top level: SAMT
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_J45001/annotation1880597/range1880597/1

https://synbiohub.org/public/igem/BBa_J45001/annotation1880597/range1880597/1
type: Range
persistent identity: range1880597
display id: range1880597
version: 1
direction: direction/forward
top level: SAMT
start: 1150
end: 1155
orientation: inline

COMT
type: ComponentDefinition
persistent identity: BBa I742107
display id: BBa_I742107
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: COMT
creator: sarah hollingshead
created: 2007-10-18T11:00:00Z
modified: 2015-08-31T04:08:02Z
discontinued: false
dominant: false
experience: experience/None
group u list: _123_
m user id: 0
owner id: 1964
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: COMT coding sequence Encodes caffeic acid-O-methyl transferase (methylates -OH on C4 of the aromatic ring) from Medicago sativa (alfalfa), synthesised by Geneart
mutable notes: Used cDNA as coding gene in Alfalfa genome contains introns.
mutable provenance: Alfalfa cDNA Genes and Enzymes Involved in Caffeic Acid Biosynthesis in the Actinomycete Saccharothrix esanaensis, Berner M, et al, Journal of Bacteriology 188(7): 2666, (2006) Gowri G, et al, Molecular cloning and expression of alfalfa S-adenosyl-L-methionine: caffeic acid 3-0-methyltransferase, a key enzyme of lignin biosynthesis, Plant Physiology 97(1):7 (1991) Downregulation of Caffeic Acid 3-O-Methyltransferase and Caffeoyl CoA 3-O-Methyltransferase in Transgenic Alfalfa Impacts on Lignin Structure and Implications for the Biosynthesis of G and S Lignin, Guo D, et al, Plant Cell. January; 13(1): 73 (2001)
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: COMT coding region
sequence: https://synbiohub.org/public/igem/BBa_I742107_sequence/1

COMT coding region
type: SequenceAnnotation
persistent identity: annotation1949817
display id: annotation1949817
version: 1
top level: COMT
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_I742107/annotation1949817/range1949817/1

https://synbiohub.org/public/igem/BBa_I742107/annotation1949817/range1949817/1
type: Range
persistent identity: range1949817
display id: range1949817
version: 1
direction: direction/forward
top level: COMT
start: 1
end: 1101
orientation: inline

BBa_J45298
type: ComponentDefinition
persistent identity: BBa J45298
display id: BBa_J45298
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PchA & PchB enzyme generator
creator: Andr?? Green II
created: 2006-10-27T11:00:00Z
modified: 2015-08-31T04:08:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _84_
m user id: 0
owner id: 977
owning group id: 84
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part is an intermediate to the protein generator BBa_45300.
mutable notes: coming
mutable provenance: coming
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_J45017
component: BBa_B0010
component: BBa_B0012
sequence annotation: BBa_B0012
sequence annotation: BBa_J45017
sequence annotation: BBa_B0010
sequence: https://synbiohub.org/public/igem/BBa_J45298_sequence/1

BBa_J45017
type: Component
persistent identity: component1960908
display id: component1960908
version: 1
top level: BBa_J45298
access: public
definition: pchBA

BBa_B0010
type: Component
persistent identity: component1960909
display id: component1960909
version: 1
top level: BBa_J45298
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
type: Component
persistent identity: component1960911
display id: component1960911
version: 1
top level: BBa_J45298
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1960911
display id: annotation1960911
version: 1
top level: BBa_J45298
location: https://synbiohub.org/public/igem/BBa_J45298/annotation1960911/range1960911/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_J45298/annotation1960911/range1960911/1
type: Range
persistent identity: range1960911
display id: range1960911
version: 1
direction: direction/forward
top level: BBa_J45298
start: 1833
end: 1873
orientation: inline

BBa_J45017
type: SequenceAnnotation
persistent identity: annotation1960908
display id: annotation1960908
version: 1
top level: BBa_J45298
location: https://synbiohub.org/public/igem/BBa_J45298/annotation1960908/range1960908/1
component: BBa_J45017

https://synbiohub.org/public/igem/BBa_J45298/annotation1960908/range1960908/1
type: Range
persistent identity: range1960908
display id: range1960908
version: 1
direction: direction/reverse
top level: BBa_J45298
start: 1
end: 1736
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1960909
display id: annotation1960909
version: 1
top level: BBa_J45298
location: https://synbiohub.org/public/igem/BBa_J45298/annotation1960909/range1960909/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_J45298/annotation1960909/range1960909/1
type: Range
persistent identity: range1960909
display id: range1960909
version: 1
direction: direction/forward
top level: BBa_J45298
start: 1745
end: 1824
orientation: inline

BAT2
type: ComponentDefinition
persistent identity: BBa J45008
display id: BBa_J45008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: branched-chain amino acid transaminase (BAT2); converts leucine to alpha-ketoisocaproate
creator: Veena Venkatachalam
created: 2006-06-06T11:00:00Z
modified: 2015-08-31T04:08:49Z
discontinued: false
dominant: false
experience: experience/Fails
group u list: _84_
m user id: 0
owner id: 642
owning group id: 84
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: fd;lsfas
mutable notes: dakfdas;
mutable provenance: dkfkas
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: start codon
sequence annotation: double TAA stop codon
sequence annotation: a->g to eliminate SpeI
sequence annotation: BAT2
sequence: https://synbiohub.org/public/igem/BBa_J45008_sequence/1

start codon
type: SequenceAnnotation
persistent identity: annotation1883624
display id: annotation1883624
version: 1
top level: BAT2
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_J45008/annotation1883624/range1883624/1

https://synbiohub.org/public/igem/BBa_J45008/annotation1883624/range1883624/1
type: Range
persistent identity: range1883624
display id: range1883624
version: 1
direction: direction/forward
top level: BAT2
start: 1
end: 3
orientation: inline

double TAA stop codon
type: SequenceAnnotation
persistent identity: annotation1883625
display id: annotation1883625
version: 1
top level: BAT2
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_J45008/annotation1883625/range1883625/1

https://synbiohub.org/public/igem/BBa_J45008/annotation1883625/range1883625/1
type: Range
persistent identity: range1883625
display id: range1883625
version: 1
direction: direction/forward
top level: BAT2
start: 1129
end: 1134
orientation: inline

a->g to eliminate SpeI
type: SequenceAnnotation
persistent identity: annotation1894553
display id: annotation1894553
version: 1
top level: BAT2
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_J45008/annotation1894553/range1894553/1

https://synbiohub.org/public/igem/BBa_J45008/annotation1894553/range1894553/1
type: Range
persistent identity: range1894553
display id: range1894553
version: 1
direction: direction/forward
top level: BAT2
start: 771
end: 771
orientation: inline

BAT2
type: SequenceAnnotation
persistent identity: annotation1961299
display id: annotation1961299
version: 1
top level: BAT2
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J45008/annotation1961299/range1961299/1

https://synbiohub.org/public/igem/BBa_J45008/annotation1961299/range1961299/1
type: Range
persistent identity: range1961299
display id: range1961299
version: 1
direction: direction/forward
top level: BAT2
start: 1
end: 1134
orientation: inline

ATF1
type: ComponentDefinition
persistent identity: BBa J45014
display id: BBa_J45014
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: alcohol acetyltransferase I; converts isoamyl alcohol to isoamyl acetate (banana odor)
creator: Andr?? Green II
created: 2006-07-30T11:00:00Z
modified: 2015-08-31T04:08:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _84_
m user id: 0
owner id: 977
owning group id: 84
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: This part is the ATF sequence (BBa_45J006) with a single point mutation at base pair 1176 (T -> C).
mutable notes: It has a single point mutation at base pair 1176 (T -> C).
mutable provenance: This part comes from BBa_45J006.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: double TAA stop codon
sequence annotation: t -> c
sequence annotation: ATF1
sequence annotation: a->g to eliminate EcoRI site
sequence: https://synbiohub.org/public/igem/BBa_J45014_sequence/1

double TAA stop codon
type: SequenceAnnotation
persistent identity: annotation1961302
display id: annotation1961302
version: 1
top level: ATF1
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_J45014/annotation1961302/range1961302/1

https://synbiohub.org/public/igem/BBa_J45014/annotation1961302/range1961302/1
type: Range
persistent identity: range1961302
display id: range1961302
version: 1
direction: direction/forward
top level: ATF1
start: 1576
end: 1581
orientation: inline

t -> c
type: SequenceAnnotation
persistent identity: annotation1961301
display id: annotation1961301
version: 1
top level: ATF1
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_J45014/annotation1961301/range1961301/1

https://synbiohub.org/public/igem/BBa_J45014/annotation1961301/range1961301/1
type: Range
persistent identity: range1961301
display id: range1961301
version: 1
direction: direction/forward
top level: ATF1
start: 1148
end: 1148
orientation: inline

ATF1
type: SequenceAnnotation
persistent identity: annotation1961300
display id: annotation1961300
version: 1
top level: ATF1
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J45014/annotation1961300/range1961300/1

https://synbiohub.org/public/igem/BBa_J45014/annotation1961300/range1961300/1
type: Range
persistent identity: range1961300
display id: range1961300
version: 1
direction: direction/forward
top level: ATF1
start: 1
end: 1581
orientation: inline

a->g to eliminate EcoRI site
type: SequenceAnnotation
persistent identity: annotation1961305
display id: annotation1961305
version: 1
top level: ATF1
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_J45014/annotation1961305/range1961305/1

https://synbiohub.org/public/igem/BBa_J45014/annotation1961305/range1961305/1
type: Range
persistent identity: range1961305
display id: range1961305
version: 1
direction: direction/forward
top level: ATF1
start: 414
end: 414
orientation: inline

BBa_J45098
type: ComponentDefinition
persistent identity: BBa J45098
display id: BBa_J45098
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: BSMT+Terminator
creator: Boyuan Zhu
created: 2006-08-08T11:00:00Z
modified: 2015-08-31T04:08:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _84_
m user id: 0
owner id: 974
owning group id: 84
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This is an intermediate with BSMT hooked up to a terminator. It can be hooked up to a variety of RBS and promoters. This is an early step in creating a "wintergreen generating machine".
mutable notes: We made (cut) a B0015 backbone for this part.
mutable provenance: It comes from the previous biobrick parts J45004 and B0015.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_J45004
component: BBa_B0010
component: BBa_B0012
sequence annotation: BBa_J45004
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_J45098_sequence/1

BBa_J45004
type: Component
persistent identity: component1894557
display id: component1894557
version: 1
top level: BBa_J45098
access: public
definition: BSMT1

BBa_B0010
type: Component
persistent identity: component1894558
display id: component1894558
version: 1
top level: BBa_J45098
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
type: Component
persistent identity: component1894560
display id: component1894560
version: 1
top level: BBa_J45098
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_J45004
type: SequenceAnnotation
persistent identity: annotation1894557
display id: annotation1894557
version: 1
top level: BBa_J45098
location: https://synbiohub.org/public/igem/BBa_J45098/annotation1894557/range1894557/1
component: BBa_J45004

https://synbiohub.org/public/igem/BBa_J45098/annotation1894557/range1894557/1
type: Range
persistent identity: range1894557
display id: range1894557
version: 1
direction: direction/reverse
top level: BBa_J45098
start: 1
end: 1074
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1894558
display id: annotation1894558
version: 1
top level: BBa_J45098
location: https://synbiohub.org/public/igem/BBa_J45098/annotation1894558/range1894558/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_J45098/annotation1894558/range1894558/1
type: Range
persistent identity: range1894558
display id: range1894558
version: 1
direction: direction/forward
top level: BBa_J45098
start: 1083
end: 1162
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1894560
display id: annotation1894560
version: 1
top level: BBa_J45098
location: https://synbiohub.org/public/igem/BBa_J45098/annotation1894560/range1894560/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_J45098/annotation1894560/range1894560/1
type: Range
persistent identity: range1894560
display id: range1894560
version: 1
direction: direction/forward
top level: BBa_J45098
start: 1171
end: 1211
orientation: inline

BBa_K1660003
type: ComponentDefinition
persistent identity: BBa K1660003
display id: BBa_K1660003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: d-limonene synthase
creator: Dai Yuanyi
created: 2015-09-06T11:00:00Z
modified: 2015-09-07T10:42:36Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2078_
m user id: 26933
owner id: 26933
owning group id: 9
status: status/Planning
bookmark: true
mutable description: This part can express d-limonene synthase with a constitutive promoter J23100, can it can catalyze GPPs into d-limonenen. We use this part to produce d-limonene which is said can attract nematode and some other pests well.
mutable notes: We searched the cDNA sequence in NCBI, and optimized the sequence for E. coli. Meanwhile, we inserted a Xho I restriction site between RBS and d-limonene synthase coding sequence, so that we can change promoter easily.
mutable provenance: It comes from Citrus unshiu CitMTSE1 d-limonene synthase.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: start codon
sequence annotation: stop codon
sequence annotation: J23100
sequence annotation: d-limonene synthase
sequence annotation: B0032
sequence: https://synbiohub.org/public/igem/BBa_K1660003_sequence/1

start codon
type: SequenceAnnotation
persistent identity: annotation2445818
display id: annotation2445818
version: 1
top level: BBa_K1660003
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1660003/annotation2445818/range2445818/1

https://synbiohub.org/public/igem/BBa_K1660003/annotation2445818/range2445818/1
type: Range
persistent identity: range2445818
display id: range2445818
version: 1
direction: direction/forward
top level: BBa_K1660003
start: 63
end: 65
orientation: inline

stop codon
type: SequenceAnnotation
persistent identity: annotation2445820
display id: annotation2445820
version: 1
top level: BBa_K1660003
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1660003/annotation2445820/range2445820/1

https://synbiohub.org/public/igem/BBa_K1660003/annotation2445820/range2445820/1
type: Range
persistent identity: range2445820
display id: range2445820
version: 1
direction: direction/forward
top level: BBa_K1660003
start: 1881
end: 1883
orientation: inline

J23100
type: SequenceAnnotation
persistent identity: annotation2445816
display id: annotation2445816
version: 1
top level: BBa_K1660003
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1660003/annotation2445816/range2445816/1

https://synbiohub.org/public/igem/BBa_K1660003/annotation2445816/range2445816/1
type: Range
persistent identity: range2445816
display id: range2445816
version: 1
direction: direction/forward
top level: BBa_K1660003
start: 1
end: 35
orientation: inline

d-limonene synthase
type: SequenceAnnotation
persistent identity: annotation2445819
display id: annotation2445819
version: 1
top level: BBa_K1660003
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1660003/annotation2445819/range2445819/1

https://synbiohub.org/public/igem/BBa_K1660003/annotation2445819/range2445819/1
type: Range
persistent identity: range2445819
display id: range2445819
version: 1
direction: direction/forward
top level: BBa_K1660003
start: 63
end: 1883
orientation: inline

B0032
type: SequenceAnnotation
persistent identity: annotation2445817
display id: annotation2445817
version: 1
top level: BBa_K1660003
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1660003/annotation2445817/range2445817/1

https://synbiohub.org/public/igem/BBa_K1660003/annotation2445817/range2445817/1
type: Range
persistent identity: range2445817
display id: range2445817
version: 1
direction: direction/forward
top level: BBa_K1660003
start: 44
end: 46
orientation: inline

BSMT1
type: ComponentDefinition
persistent identity: BBa J45004
display id: BBa_J45004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: SAM:benzoic acid/salicylic acid carboxyl methyltransferase I; converts salicylic acid to methyl sali
creator: Boyuan Zhu
created: 2006-06-06T11:00:00Z
modified: 2015-08-31T04:08:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _84_
m user id: 0
owner id: 974
owning group id: 84
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: Codes for BSMT enzyme to convert Benzoic acid/salicylic acid to methyl benzoate and methyl salicylate. This part is phBSMT1 from Petunia x hybrida.
mutable notes: Transport issues are being considered.
mutable provenance: Species: Petunia x hybrida from Genbank; (also available: A. thaliana and N. suaveolens)
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: stop codon
sequence annotation: BMST1
sequence annotation: start codon
sequence: https://synbiohub.org/public/igem/BBa_J45004_sequence/1

stop codon
type: SequenceAnnotation
persistent identity: annotation1880520
display id: annotation1880520
version: 1
top level: BSMT1
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_J45004/annotation1880520/range1880520/1

https://synbiohub.org/public/igem/BBa_J45004/annotation1880520/range1880520/1
type: Range
persistent identity: range1880520
display id: range1880520
version: 1
direction: direction/forward
top level: BSMT1
start: 1072
end: 1074
orientation: inline

BMST1
type: SequenceAnnotation
persistent identity: annotation1880517
display id: annotation1880517
version: 1
top level: BSMT1
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J45004/annotation1880517/range1880517/1

https://synbiohub.org/public/igem/BBa_J45004/annotation1880517/range1880517/1
type: Range
persistent identity: range1880517
display id: range1880517
version: 1
direction: direction/forward
top level: BSMT1
start: 1
end: 1074
orientation: inline

start codon
type: SequenceAnnotation
persistent identity: annotation1880519
display id: annotation1880519
version: 1
top level: BSMT1
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_J45004/annotation1880519/range1880519/1

https://synbiohub.org/public/igem/BBa_J45004/annotation1880519/range1880519/1
type: Range
persistent identity: range1880519
display id: range1880519
version: 1
direction: direction/forward
top level: BSMT1
start: 1
end: 3
orientation: inline