//function/biosynthesis/ahl
type: Collection
persistent identity: function biosynthesis ahl
display id: function_biosynthesis_ahl
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
owned by: james
owned by: myers
member: BBa_K1520019
member: cinI
member: rhII
member: BBa_I0460
member: BBa_C0261
member: BBa_K546000
member: BBa_I722012
member: BBa_K1520022
member: BBa_K091109
member: BBa_I722011
member: BBa_K082008
member: BBa_K1520511
member: luxI
member: lasI
member: BBa_K082023
member: aiiA
member: BBa_K082010
member: BBa_K1520512
member: BBa_K1342001
member: BBa_K1520009
member: BBa_K1520011
member: BBa_K1520504
member: BBa_K082007
member: BBa_K082035
member: BBa_K1520508
member: BBa_K082031
member: BBa_K1520510
member: BBa_I9026
member: BBa_K082032
member: BBa_K546005

BBa_K082031
type: ComponentDefinition
persistent identity: BBa K082031
display id: BBa_K082031
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: R0071-B0030-C0070
creator: Zhen Xu , Binjie Xu
created: 2008-10-27T12:00:00Z
modified: 2015-05-08T01:08:36Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _228_
m user id: 0
owner id: 1560
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: none
mutable notes: none
mutable provenance: none
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_R0071
component: BBa_B0030
component: BBa_C0070
sequence annotation: BBa_C0070
sequence annotation: BBa_B0030
sequence annotation: BBa_R0071
sequence: https://synbiohub.org/public/igem/BBa_K082031_sequence/1

BBa_R0071
type: Component
persistent identity: component2246236
display id: component2246236
version: 1
top level: BBa_K082031
access: public
definition: https://synbiohub.org/public/igem/BBa_R0071/1

BBa_B0030
type: Component
persistent identity: component2246238
display id: component2246238
version: 1
top level: BBa_K082031
access: public
definition: https://synbiohub.org/public/igem/BBa_B0030/1

BBa_C0070
type: Component
persistent identity: component2246243
display id: component2246243
version: 1
top level: BBa_K082031
access: public
definition: rhII

BBa_C0070
type: SequenceAnnotation
persistent identity: annotation2246243
display id: annotation2246243
version: 1
top level: BBa_K082031
location: https://synbiohub.org/public/igem/BBa_K082031/annotation2246243/range2246243/1
component: BBa_C0070

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type: Range
persistent identity: range2246243
display id: range2246243
version: 1
direction: direction/reverse
top level: BBa_K082031
start: 83
end: 749
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation2246238
display id: annotation2246238
version: 1
top level: BBa_K082031
location: https://synbiohub.org/public/igem/BBa_K082031/annotation2246238/range2246238/1
component: BBa_B0030

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type: Range
persistent identity: range2246238
display id: range2246238
version: 1
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top level: BBa_K082031
start: 62
end: 76
orientation: inline

BBa_R0071
type: SequenceAnnotation
persistent identity: annotation2246236
display id: annotation2246236
version: 1
top level: BBa_K082031
location: https://synbiohub.org/public/igem/BBa_K082031/annotation2246236/range2246236/1
component: BBa_R0071

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type: Range
persistent identity: range2246236
display id: range2246236
version: 1
direction: direction/reverse
top level: BBa_K082031
start: 1
end: 53
orientation: inline

BBa_K082023
type: ComponentDefinition
persistent identity: BBa K082023
display id: BBa_K082023
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: B0034-C0070-B0015
creator: Zhen Xu, Binjie Xu
created: 2008-10-28T12:00:00Z
modified: 2015-05-08T01:08:35Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _228_
m user id: 0
owner id: 1560
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: none
mutable notes: none
mutable provenance: none
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0015
component: BBa_C0070
component: BBa_B0034
sequence annotation: BBa_B0034
sequence annotation: BBa_C0070
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_K082023_sequence/1

BBa_B0015
type: Component
persistent identity: component2249414
display id: component2249414
version: 1
top level: BBa_K082023
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_C0070
type: Component
persistent identity: component2249407
display id: component2249407
version: 1
top level: BBa_K082023
access: public
definition: rhII

BBa_B0034
type: Component
persistent identity: component2249403
display id: component2249403
version: 1
top level: BBa_K082023
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2249403
display id: annotation2249403
version: 1
top level: BBa_K082023
location: https://synbiohub.org/public/igem/BBa_K082023/annotation2249403/range2249403/1
component: BBa_B0034

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type: Range
persistent identity: range2249403
display id: range2249403
version: 1
direction: direction/reverse
top level: BBa_K082023
start: 1
end: 12
orientation: inline

BBa_C0070
type: SequenceAnnotation
persistent identity: annotation2249407
display id: annotation2249407
version: 1
top level: BBa_K082023
location: https://synbiohub.org/public/igem/BBa_K082023/annotation2249407/range2249407/1
component: BBa_C0070

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type: Range
persistent identity: range2249407
display id: range2249407
version: 1
direction: direction/reverse
top level: BBa_K082023
start: 19
end: 685
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2249414
display id: annotation2249414
version: 1
top level: BBa_K082023
location: https://synbiohub.org/public/igem/BBa_K082023/annotation2249414/range2249414/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K082023/annotation2249414/range2249414/1
type: Range
persistent identity: range2249414
display id: range2249414
version: 1
direction: direction/reverse
top level: BBa_K082023
start: 694
end: 822
orientation: inline

BBa_K1520019
type: ComponentDefinition
persistent identity: BBa K1520019
display id: BBa_K1520019
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Pcons2-rbs-lacI-Ter-Plac-rbs-luxI-Ter
creator: yue wu
created: 2014-09-21T11:00:00Z
modified: 2015-05-08T01:10:49Z
discontinued: false
dominant: false
group u list: _1902_
m user id: 0
owner id: 21318
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Pcons2-rbs-lacI-Ter-Plac-rbs-luxI-Ter It produces luxI when there is enough IPTG.
mutable notes: None
mutable provenance: Pcons2, lacI, Plac, luxI, Ter
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1520008
component: BBa_K1520009
sequence annotation: BBa_K1520008
sequence annotation: BBa_K1520009
sequence: https://synbiohub.org/public/igem/BBa_K1520019_sequence/1

BBa_K1520008
type: Component
persistent identity: component2385079
display id: component2385079
version: 1
top level: BBa_K1520019
access: public
definition: https://synbiohub.org/public/igem/BBa_K1520008/1

BBa_K1520009
type: Component
persistent identity: component2385091
display id: component2385091
version: 1
top level: BBa_K1520019
access: public
definition: BBa_K1520009

BBa_K1520008
type: SequenceAnnotation
persistent identity: annotation2385079
display id: annotation2385079
version: 1
top level: BBa_K1520019
location: https://synbiohub.org/public/igem/BBa_K1520019/annotation2385079/range2385079/1
component: BBa_K1520008

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type: Range
persistent identity: range2385079
display id: range2385079
version: 1
direction: direction/reverse
top level: BBa_K1520019
start: 1
end: 1351
orientation: inline

BBa_K1520009
type: SequenceAnnotation
persistent identity: annotation2385091
display id: annotation2385091
version: 1
top level: BBa_K1520019
location: https://synbiohub.org/public/igem/BBa_K1520019/annotation2385091/range2385091/1
component: BBa_K1520009

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type: Range
persistent identity: range2385091
display id: range2385091
version: 1
direction: direction/reverse
top level: BBa_K1520019
start: 1360
end: 2365
orientation: inline

lasI
type: ComponentDefinition
persistent identity: BBa C0078
display id: BBa_C0078
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: autoinducer synthetase for PAI from Pseudomonas aeruginosa
creator: Chris Walsh (Fighting Darwins)
created: 2004-01-27T12:00:00Z
modified: 2015-08-31T04:07:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: coding region for lasI protein, which produces the chemical signal AI-1
mutable provenance: www.ncbi.nlm.nih.gov
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: LasI
sequence annotation: Help:Barcodes
sequence annotation: LVA
sequence: https://synbiohub.org/public/igem/BBa_C0078_sequence/1

LasI
type: SequenceAnnotation
persistent identity: annotation305970
display id: annotation305970
version: 1
top level: lasI
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_C0078/annotation305970/range305970/1

https://synbiohub.org/public/igem/BBa_C0078/annotation305970/range305970/1
type: Range
persistent identity: range305970
display id: range305970
version: 1
direction: direction/forward
top level: lasI
start: 1
end: 603
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2214003
display id: annotation2214003
version: 1
top level: lasI
role: SO:0000807
role: feature/barcode
location: https://synbiohub.org/public/igem/BBa_C0078/annotation2214003/range2214003/1

https://synbiohub.org/public/igem/BBa_C0078/annotation2214003/range2214003/1
type: Range
persistent identity: range2214003
display id: range2214003
version: 1
direction: direction/forward
top level: lasI
start: 643
end: 667
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation306608
display id: annotation306608
version: 1
top level: lasI
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_C0078/annotation306608/range306608/1

https://synbiohub.org/public/igem/BBa_C0078/annotation306608/range306608/1
type: Range
persistent identity: range306608
display id: range306608
version: 1
direction: direction/forward
top level: lasI
start: 604
end: 636
orientation: inline

BBa_K1520512
type: ComponentDefinition
persistent identity: BBa K1520512
display id: BBa_K1520512
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: MC31-rbs-luxI-Ter
creator: yue wu
created: 2014-09-22T11:00:00Z
modified: 2015-05-08T01:10:49Z
discontinued: false
dominant: false
group u list: _1902_
m user id: 0
owner id: 21318
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: MC31-rbs-luxI-Ter If there are microcystin, the luxI will be produced.
mutable notes: None
mutable provenance: MC31, luxI, Ter
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1520504
component: BBa_K1520029
component: BBa_K1520002
sequence annotation: BBa_K1520504
sequence annotation: BBa_K1520029
sequence annotation: BBa_K1520002
sequence: https://synbiohub.org/public/igem/BBa_K1520512_sequence/1

BBa_K1520504
type: Component
persistent identity: component2385598
display id: component2385598
version: 1
top level: BBa_K1520512
access: public
definition: BBa_K1520504

BBa_K1520029
type: Component
persistent identity: component2385599
display id: component2385599
version: 1
top level: BBa_K1520512
access: public
definition: https://synbiohub.org/public/igem/BBa_K1520029/1

BBa_K1520002
type: Component
persistent identity: component2385595
display id: component2385595
version: 1
top level: BBa_K1520512
access: public
definition: https://synbiohub.org/public/igem/BBa_K1520002/1

BBa_K1520504
type: SequenceAnnotation
persistent identity: annotation2385598
display id: annotation2385598
version: 1
top level: BBa_K1520512
location: https://synbiohub.org/public/igem/BBa_K1520512/annotation2385598/range2385598/1
component: BBa_K1520504

https://synbiohub.org/public/igem/BBa_K1520512/annotation2385598/range2385598/1
type: Range
persistent identity: range2385598
display id: range2385598
version: 1
direction: direction/reverse
top level: BBa_K1520512
start: 141
end: 801
orientation: inline

BBa_K1520029
type: SequenceAnnotation
persistent identity: annotation2385599
display id: annotation2385599
version: 1
top level: BBa_K1520512
location: https://synbiohub.org/public/igem/BBa_K1520512/annotation2385599/range2385599/1
component: BBa_K1520029

https://synbiohub.org/public/igem/BBa_K1520512/annotation2385599/range2385599/1
type: Range
persistent identity: range2385599
display id: range2385599
version: 1
direction: direction/reverse
top level: BBa_K1520512
start: 810
end: 938
orientation: inline

BBa_K1520002
type: SequenceAnnotation
persistent identity: annotation2385595
display id: annotation2385595
version: 1
top level: BBa_K1520512
location: https://synbiohub.org/public/igem/BBa_K1520512/annotation2385595/range2385595/1
component: BBa_K1520002

https://synbiohub.org/public/igem/BBa_K1520512/annotation2385595/range2385595/1
type: Range
persistent identity: range2385595
display id: range2385595
version: 1
direction: direction/reverse
top level: BBa_K1520512
start: 1
end: 132
orientation: inline

BBa_I9026
type: ComponentDefinition
persistent identity: BBa I9026
display id: BBa_I9026
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: rhlI translational unit
creator: crackdots
created: 2004-01-27T12:00:00Z
modified: 2015-08-31T04:08:11Z
discontinued: false
dominant: true
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_C0070
component: BBa_B0034
sequence annotation: BBa_C0070
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_I9026_sequence/1

BBa_C0070
type: Component
persistent identity: component939656
display id: component939656
version: 1
top level: BBa_I9026
access: public
definition: rhII

BBa_B0034
type: Component
persistent identity: component939646
display id: component939646
version: 1
top level: BBa_I9026
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_C0070
type: SequenceAnnotation
persistent identity: annotation939656
display id: annotation939656
version: 1
top level: BBa_I9026
location: https://synbiohub.org/public/igem/BBa_I9026/annotation939656/range939656/1
component: BBa_C0070

https://synbiohub.org/public/igem/BBa_I9026/annotation939656/range939656/1
type: Range
persistent identity: range939656
display id: range939656
version: 1
direction: direction/forward
top level: BBa_I9026
start: 19
end: 660
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation939646
display id: annotation939646
version: 1
top level: BBa_I9026
location: https://synbiohub.org/public/igem/BBa_I9026/annotation939646/range939646/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I9026/annotation939646/range939646/1
type: Range
persistent identity: range939646
display id: range939646
version: 1
direction: direction/forward
top level: BBa_I9026
start: 1
end: 12
orientation: inline

BBa_I0460
type: ComponentDefinition
persistent identity: BBa I0460
display id: BBa_I0460
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: aiiA Device (B0034.C0060.B0015)
creator: Caitlin Conboy and Jennifer Braff
created: 2003-12-04T12:00:00Z
modified: 2015-08-31T04:07:29Z
discontinued: false
dominant: true
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: -- No description --
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0034
component: BBa_B0012
component: BBa_B0010
component: BBa_C0060
sequence annotation: BBa_C0060
sequence annotation: BBa_B0034
sequence annotation: BBa_B0012
sequence annotation: BBa_B0010
sequence: https://synbiohub.org/public/igem/BBa_I0460_sequence/1

BBa_B0034
type: Component
persistent identity: component943094
display id: component943094
version: 1
top level: BBa_I0460
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0012
type: Component
persistent identity: component943127
display id: component943127
version: 1
top level: BBa_I0460
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0010
type: Component
persistent identity: component943117
display id: component943117
version: 1
top level: BBa_I0460
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_C0060
type: Component
persistent identity: component943109
display id: component943109
version: 1
top level: BBa_I0460
access: public
definition: aiiA

BBa_C0060
type: SequenceAnnotation
persistent identity: annotation943109
display id: annotation943109
version: 1
top level: BBa_I0460
location: https://synbiohub.org/public/igem/BBa_I0460/annotation943109/range943109/1
component: BBa_C0060

https://synbiohub.org/public/igem/BBa_I0460/annotation943109/range943109/1
type: Range
persistent identity: range943109
display id: range943109
version: 1
direction: direction/forward
top level: BBa_I0460
start: 19
end: 807
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation943094
display id: annotation943094
version: 1
top level: BBa_I0460
location: https://synbiohub.org/public/igem/BBa_I0460/annotation943094/range943094/1
component: BBa_B0034

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type: Range
persistent identity: range943094
display id: range943094
version: 1
direction: direction/forward
top level: BBa_I0460
start: 1
end: 12
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation943127
display id: annotation943127
version: 1
top level: BBa_I0460
location: https://synbiohub.org/public/igem/BBa_I0460/annotation943127/range943127/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I0460/annotation943127/range943127/1
type: Range
persistent identity: range943127
display id: range943127
version: 1
direction: direction/forward
top level: BBa_I0460
start: 929
end: 969
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation943117
display id: annotation943117
version: 1
top level: BBa_I0460
location: https://synbiohub.org/public/igem/BBa_I0460/annotation943117/range943117/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I0460/annotation943117/range943117/1
type: Range
persistent identity: range943117
display id: range943117
version: 1
direction: direction/forward
top level: BBa_I0460
start: 841
end: 920
orientation: inline

BBa_K1520009
type: ComponentDefinition
persistent identity: BBa K1520009
display id: BBa_K1520009
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Plac-rbs-luxI-Ter
creator: yue wu
created: 2014-09-21T11:00:00Z
modified: 2015-05-08T01:10:49Z
discontinued: false
dominant: false
group u list: _1902_
m user id: 0
owner id: 21318
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Plac-rbs-luxI-Ter. It produces luxI when there is enough IPTG or less lacI.
mutable notes: None
mutable provenance: Plac, luxI, Ter
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1520029
component: BBa_R0010
component: BBa_K1520504
sequence annotation: BBa_K1520504
sequence annotation: BBa_K1520029
sequence annotation: BBa_R0010
sequence: https://synbiohub.org/public/igem/BBa_K1520009_sequence/1

BBa_K1520029
type: Component
persistent identity: component2384947
display id: component2384947
version: 1
top level: BBa_K1520009
access: public
definition: https://synbiohub.org/public/igem/BBa_K1520029/1

BBa_R0010
type: Component
persistent identity: component2384937
display id: component2384937
version: 1
top level: BBa_K1520009
access: public
definition: https://synbiohub.org/public/igem/BBa_R0010/1

BBa_K1520504
type: Component
persistent identity: component2384946
display id: component2384946
version: 1
top level: BBa_K1520009
access: public
definition: BBa_K1520504

BBa_K1520504
type: SequenceAnnotation
persistent identity: annotation2384946
display id: annotation2384946
version: 1
top level: BBa_K1520009
location: https://synbiohub.org/public/igem/BBa_K1520009/annotation2384946/range2384946/1
component: BBa_K1520504

https://synbiohub.org/public/igem/BBa_K1520009/annotation2384946/range2384946/1
type: Range
persistent identity: range2384946
display id: range2384946
version: 1
direction: direction/reverse
top level: BBa_K1520009
start: 209
end: 869
orientation: inline

BBa_K1520029
type: SequenceAnnotation
persistent identity: annotation2384947
display id: annotation2384947
version: 1
top level: BBa_K1520009
location: https://synbiohub.org/public/igem/BBa_K1520009/annotation2384947/range2384947/1
component: BBa_K1520029

https://synbiohub.org/public/igem/BBa_K1520009/annotation2384947/range2384947/1
type: Range
persistent identity: range2384947
display id: range2384947
version: 1
direction: direction/reverse
top level: BBa_K1520009
start: 878
end: 1006
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation2384937
display id: annotation2384937
version: 1
top level: BBa_K1520009
location: https://synbiohub.org/public/igem/BBa_K1520009/annotation2384937/range2384937/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_K1520009/annotation2384937/range2384937/1
type: Range
persistent identity: range2384937
display id: range2384937
version: 1
direction: direction/forward
top level: BBa_K1520009
start: 1
end: 200
orientation: inline

aiiA
type: ComponentDefinition
persistent identity: BBa C0060
display id: BBa_C0060
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: autoinducer inactivation enzyme from Bacillus; hydrolyzes acetyl homoserine lactone
creator: Vinay S Mahajan, Brian Chow, Peter Carr, Voichita D. Marinescu and Alexander D. Wissner-Gross
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:23Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Coding region for the autoinducer inactivation enzyme A (aiiA) LVA tagged. The gene was originally isolated from Bacillus sp. 240B1 and it encodes an enzyme that catalyzes the degradation of N-acyl homoserine lactones (AHLs)--quorum sensing autoinducers.
mutable notes: References (unparsed) here: Dong,Y.H., Xu,J.L., Li,X.Z. and Zhang,L.H.: AiiA, an enzyme that inactivates the acylhomoserine lactone quorum-sensing signal and attenuates the virulence of Erwinia carotovora, Proc. Natl. Acad. Sci. U.S.A. 97 (7), 3526-3531 (2000). http://www.pnas.org/cgi/content/full/97/7/3526 Lee SJ, Park SY, Lee JJ, Yum DY, Koo BT, Lee JK.: Genes encoding the N-acyl homoserine lactone-degrading enzyme are widespread in many subspecies of Bacillus thuringiensis, Appl Environ Microbiol 2002 Aug;68(8):3919-24. http://aem.asm.org/cgi/content/full/68/8/3919?view=full&pmid=12147491 References (unparsed) here: Dong,Y.H., Xu,J.L., Li,X.Z. and Zhang,L.H.: AiiA, an enzyme that inactivates the acylhomoserine lactone quorum-sensing signal and attenuates the virulence of Erwinia carotovora, Proc. Natl. Acad. Sci. U.S.A. 97 (7), 3526-3531 (2000). http://www.pnas.org/cgi/content/full/97/7/3526 Lee SJ, Park SY, Lee JJ, Yum DY, Koo BT, Lee JK.: Genes encoding the N-acyl homoserine lactone-degrading enzyme are widespread in many subspecies of Bacillus thuringiensis, Appl Environ Microbiol 2002 Aug;68(8):3919-24. http://aem.asm.org/cgi/content/full/68/8/3919?view=full&pmid=12147491 BBa_C0060 insert contains open reading frame (nucleotides 49-801) of the GeneBank sequence AF196486 followed by the LVA tag and two double stop codons inserted in the BioBrick prefix and suffix flanking regions. The original stop codon was TAG and in the present sequence it was substituted by TAATAA.
mutable provenance: <genbank>AF196486</genbank> from Bacillus sp. 240B1 putative metallohydrolase (aiiA) gene. Dong,Y.H., Xu,J.L., Li,X.Z. and Zhang,L.H.: AiiA, an enzyme that inactivates the acylhomoserine lactone quorum-sensing signal and attenuates the virulence of Erwinia carotovora, Proc. Natl. Acad. Sci. U.S.A. 97 (7), 3526-3531 (2000).
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: LVA
sequence annotation: aiiA
sequence annotation: BBa_C0060
sequence annotation: Help:Barcodes
sequence annotation: start
sequence annotation: 2
sequence: https://synbiohub.org/public/igem/BBa_C0060_sequence/1

LVA
type: SequenceAnnotation
persistent identity: annotation1756
display id: annotation1756
version: 1
top level: aiiA
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_C0060/annotation1756/range1756/1

https://synbiohub.org/public/igem/BBa_C0060/annotation1756/range1756/1
type: Range
persistent identity: range1756
display id: range1756
version: 1
direction: direction/forward
top level: aiiA
start: 751
end: 783
orientation: inline

aiiA
type: SequenceAnnotation
persistent identity: annotation1757
display id: annotation1757
version: 1
top level: aiiA
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_C0060/annotation1757/range1757/1

https://synbiohub.org/public/igem/BBa_C0060/annotation1757/range1757/1
type: Range
persistent identity: range1757
display id: range1757
version: 1
direction: direction/forward
top level: aiiA
start: 1
end: 750
orientation: inline

BBa_C0060
type: SequenceAnnotation
persistent identity: annotation7037
display id: annotation7037
version: 1
top level: aiiA
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_C0060/annotation7037/range7037/1

https://synbiohub.org/public/igem/BBa_C0060/annotation7037/range7037/1
type: Range
persistent identity: range7037
display id: range7037
version: 1
direction: direction/forward
top level: aiiA
start: 1
end: 789
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213987
display id: annotation2213987
version: 1
top level: aiiA
role: SO:0000807
role: feature/barcode
location: https://synbiohub.org/public/igem/BBa_C0060/annotation2213987/range2213987/1

https://synbiohub.org/public/igem/BBa_C0060/annotation2213987/range2213987/1
type: Range
persistent identity: range2213987
display id: range2213987
version: 1
direction: direction/forward
top level: aiiA
start: 790
end: 814
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation1754
display id: annotation1754
version: 1
top level: aiiA
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_C0060/annotation1754/range1754/1

https://synbiohub.org/public/igem/BBa_C0060/annotation1754/range1754/1
type: Range
persistent identity: range1754
display id: range1754
version: 1
direction: direction/forward
top level: aiiA
start: 1
end: 3
orientation: inline

2
type: SequenceAnnotation
persistent identity: annotation1755
display id: annotation1755
version: 1
top level: aiiA
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_C0060/annotation1755/range1755/1

https://synbiohub.org/public/igem/BBa_C0060/annotation1755/range1755/1
type: Range
persistent identity: range1755
display id: range1755
version: 1
direction: direction/forward
top level: aiiA
start: 784
end: 789
orientation: inline

BBa_K1520504
type: ComponentDefinition
persistent identity: BBa K1520504
display id: BBa_K1520504
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Rbs-luxI(scarless)
creator: yue wu
created: 2014-09-21T11:00:00Z
modified: 2015-05-08T01:10:49Z
discontinued: false
dominant: false
group u list: _1902_
m user id: 0
owner id: 21318
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Rbs-luxI(scarless) It is an improvement of luxI(BBa_C0061) by adding a rbs.
mutable notes: None
mutable provenance: It is an improvement of luxI(BBa_C0061) by adding a rbs.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
sequence annotation: luxI
sequence annotation: rbs
sequence: https://synbiohub.org/public/igem/BBa_K1520504_sequence/1

luxI
type: SequenceAnnotation
persistent identity: annotation2384870
display id: annotation2384870
version: 1
top level: BBa_K1520504
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1520504/annotation2384870/range2384870/1

https://synbiohub.org/public/igem/BBa_K1520504/annotation2384870/range2384870/1
type: Range
persistent identity: range2384870
display id: range2384870
version: 1
direction: direction/forward
top level: BBa_K1520504
start: 19
end: 661
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation2384869
display id: annotation2384869
version: 1
top level: BBa_K1520504
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1520504/annotation2384869/range2384869/1

https://synbiohub.org/public/igem/BBa_K1520504/annotation2384869/range2384869/1
type: Range
persistent identity: range2384869
display id: range2384869
version: 1
direction: direction/forward
top level: BBa_K1520504
start: 1
end: 18
orientation: inline

BBa_K1520511
type: ComponentDefinition
persistent identity: BBa K1520511
display id: BBa_K1520511
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: MC7-rbs-luxI-Ter
creator: yue wu
created: 2014-09-22T11:00:00Z
modified: 2015-05-08T01:10:49Z
discontinued: false
dominant: false
group u list: _1902_
m user id: 0
owner id: 21318
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: MC7-rbs-luxI-Ter If there are microcystin, the luxI will be produced.
mutable notes: None
mutable provenance: MC7, luxI, Ter
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1520504
component: BBa_K1520029
component: BBa_K1520001
sequence annotation: BBa_K1520001
sequence annotation: BBa_K1520029
sequence annotation: BBa_K1520504
sequence: https://synbiohub.org/public/igem/BBa_K1520511_sequence/1

BBa_K1520504
type: Component
persistent identity: component2385593
display id: component2385593
version: 1
top level: BBa_K1520511
access: public
definition: BBa_K1520504

BBa_K1520029
type: Component
persistent identity: component2385594
display id: component2385594
version: 1
top level: BBa_K1520511
access: public
definition: https://synbiohub.org/public/igem/BBa_K1520029/1

BBa_K1520001
type: Component
persistent identity: component2385590
display id: component2385590
version: 1
top level: BBa_K1520511
access: public
definition: https://synbiohub.org/public/igem/BBa_K1520001/1

BBa_K1520001
type: SequenceAnnotation
persistent identity: annotation2385590
display id: annotation2385590
version: 1
top level: BBa_K1520511
location: https://synbiohub.org/public/igem/BBa_K1520511/annotation2385590/range2385590/1
component: BBa_K1520001

https://synbiohub.org/public/igem/BBa_K1520511/annotation2385590/range2385590/1
type: Range
persistent identity: range2385590
display id: range2385590
version: 1
direction: direction/reverse
top level: BBa_K1520511
start: 1
end: 133
orientation: inline

BBa_K1520029
type: SequenceAnnotation
persistent identity: annotation2385594
display id: annotation2385594
version: 1
top level: BBa_K1520511
location: https://synbiohub.org/public/igem/BBa_K1520511/annotation2385594/range2385594/1
component: BBa_K1520029

https://synbiohub.org/public/igem/BBa_K1520511/annotation2385594/range2385594/1
type: Range
persistent identity: range2385594
display id: range2385594
version: 1
direction: direction/reverse
top level: BBa_K1520511
start: 811
end: 939
orientation: inline

BBa_K1520504
type: SequenceAnnotation
persistent identity: annotation2385593
display id: annotation2385593
version: 1
top level: BBa_K1520511
location: https://synbiohub.org/public/igem/BBa_K1520511/annotation2385593/range2385593/1
component: BBa_K1520504

https://synbiohub.org/public/igem/BBa_K1520511/annotation2385593/range2385593/1
type: Range
persistent identity: range2385593
display id: range2385593
version: 1
direction: direction/reverse
top level: BBa_K1520511
start: 142
end: 802
orientation: inline

BBa_K082035
type: ComponentDefinition
persistent identity: BBa K082035
display id: BBa_K082035
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RhlI generator
creator: Zhen Xu , Binjie Xu
created: 2008-10-27T12:00:00Z
modified: 2015-05-08T01:08:36Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _228_
m user id: 0
owner id: 1560
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: none
mutable notes: none
mutable provenance: none
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0034
component: BBa_C0070
component: BBa_R0040
component: BBa_B0015
sequence annotation: BBa_B0015
sequence annotation: BBa_C0070
sequence annotation: BBa_R0040
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K082035_sequence/1

BBa_B0034
type: Component
persistent identity: component2254788
display id: component2254788
version: 1
top level: BBa_K082035
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_C0070
type: Component
persistent identity: component2254792
display id: component2254792
version: 1
top level: BBa_K082035
access: public
definition: rhII

BBa_R0040
type: Component
persistent identity: component2254782
display id: component2254782
version: 1
top level: BBa_K082035
access: public
definition: https://synbiohub.org/public/igem/BBa_R0040/1

BBa_B0015
type: Component
persistent identity: component2254799
display id: component2254799
version: 1
top level: BBa_K082035
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2254799
display id: annotation2254799
version: 1
top level: BBa_K082035
location: https://synbiohub.org/public/igem/BBa_K082035/annotation2254799/range2254799/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K082035/annotation2254799/range2254799/1
type: Range
persistent identity: range2254799
display id: range2254799
version: 1
direction: direction/reverse
top level: BBa_K082035
start: 756
end: 884
orientation: inline

BBa_C0070
type: SequenceAnnotation
persistent identity: annotation2254792
display id: annotation2254792
version: 1
top level: BBa_K082035
location: https://synbiohub.org/public/igem/BBa_K082035/annotation2254792/range2254792/1
component: BBa_C0070

https://synbiohub.org/public/igem/BBa_K082035/annotation2254792/range2254792/1
type: Range
persistent identity: range2254792
display id: range2254792
version: 1
direction: direction/reverse
top level: BBa_K082035
start: 81
end: 747
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2254782
display id: annotation2254782
version: 1
top level: BBa_K082035
location: https://synbiohub.org/public/igem/BBa_K082035/annotation2254782/range2254782/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_K082035/annotation2254782/range2254782/1
type: Range
persistent identity: range2254782
display id: range2254782
version: 1
direction: direction/forward
top level: BBa_K082035
start: 1
end: 54
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2254788
display id: annotation2254788
version: 1
top level: BBa_K082035
location: https://synbiohub.org/public/igem/BBa_K082035/annotation2254788/range2254788/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K082035/annotation2254788/range2254788/1
type: Range
persistent identity: range2254788
display id: range2254788
version: 1
direction: direction/reverse
top level: BBa_K082035
start: 63
end: 74
orientation: inline

BBa_K082032
type: ComponentDefinition
persistent identity: BBa K082032
display id: BBa_K082032
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: R0071-B0032-C0070
creator: Zhen Xu , Binjie Xu
created: 2008-10-27T12:00:00Z
modified: 2015-05-08T01:08:36Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _228_
m user id: 0
owner id: 1560
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: none
mutable notes: none
mutable provenance: none
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_R0071
component: BBa_B0032
component: BBa_C0070
sequence annotation: BBa_R0071
sequence annotation: BBa_C0070
sequence annotation: BBa_B0032
sequence: https://synbiohub.org/public/igem/BBa_K082032_sequence/1

BBa_R0071
type: Component
persistent identity: component2246224
display id: component2246224
version: 1
top level: BBa_K082032
access: public
definition: https://synbiohub.org/public/igem/BBa_R0071/1

BBa_B0032
type: Component
persistent identity: component2246226
display id: component2246226
version: 1
top level: BBa_K082032
access: public
definition: https://synbiohub.org/public/igem/BBa_B0032/1

BBa_C0070
type: Component
persistent identity: component2246231
display id: component2246231
version: 1
top level: BBa_K082032
access: public
definition: rhII

BBa_R0071
type: SequenceAnnotation
persistent identity: annotation2246224
display id: annotation2246224
version: 1
top level: BBa_K082032
location: https://synbiohub.org/public/igem/BBa_K082032/annotation2246224/range2246224/1
component: BBa_R0071

https://synbiohub.org/public/igem/BBa_K082032/annotation2246224/range2246224/1
type: Range
persistent identity: range2246224
display id: range2246224
version: 1
direction: direction/reverse
top level: BBa_K082032
start: 1
end: 53
orientation: inline

BBa_C0070
type: SequenceAnnotation
persistent identity: annotation2246231
display id: annotation2246231
version: 1
top level: BBa_K082032
location: https://synbiohub.org/public/igem/BBa_K082032/annotation2246231/range2246231/1
component: BBa_C0070

https://synbiohub.org/public/igem/BBa_K082032/annotation2246231/range2246231/1
type: Range
persistent identity: range2246231
display id: range2246231
version: 1
direction: direction/reverse
top level: BBa_K082032
start: 81
end: 747
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation2246226
display id: annotation2246226
version: 1
top level: BBa_K082032
location: https://synbiohub.org/public/igem/BBa_K082032/annotation2246226/range2246226/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_K082032/annotation2246226/range2246226/1
type: Range
persistent identity: range2246226
display id: range2246226
version: 1
direction: direction/forward
top level: BBa_K082032
start: 62
end: 74
orientation: inline

BBa_K1520011
type: ComponentDefinition
persistent identity: BBa K1520011
display id: BBa_K1520011
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Prlux-rbs-rfp-rbs-luxI-Ter
creator: yue wu
created: 2014-09-21T11:00:00Z
modified: 2015-05-08T01:10:49Z
discontinued: false
dominant: false
group u list: _1902_
m user id: 0
owner id: 21318
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Prlux-rbs-rfp-rbs-luxI-Ter. It produces rfp and luxI when there is enough luxR an AHL.
mutable notes: None
mutable provenance: Prlux, rfp, luxI, Ter
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1520500
component: BBa_K1520504
component: BBa_K1520029
component: BBa_R0062
sequence annotation: BBa_K1520500
sequence annotation: BBa_K1520029
sequence annotation: BBa_R0062
sequence annotation: BBa_K1520504
sequence: https://synbiohub.org/public/igem/BBa_K1520011_sequence/1

BBa_K1520500
type: Component
persistent identity: component2384962
display id: component2384962
version: 1
top level: BBa_K1520011
access: public
definition: https://synbiohub.org/public/igem/BBa_K1520500/1

BBa_K1520504
type: Component
persistent identity: component2384965
display id: component2384965
version: 1
top level: BBa_K1520011
access: public
definition: BBa_K1520504

BBa_K1520029
type: Component
persistent identity: component2384966
display id: component2384966
version: 1
top level: BBa_K1520011
access: public
definition: https://synbiohub.org/public/igem/BBa_K1520029/1

BBa_R0062
type: Component
persistent identity: component2384957
display id: component2384957
version: 1
top level: BBa_K1520011
access: public
definition: https://synbiohub.org/public/igem/BBa_R0062/1

BBa_K1520500
type: SequenceAnnotation
persistent identity: annotation2384962
display id: annotation2384962
version: 1
top level: BBa_K1520011
location: https://synbiohub.org/public/igem/BBa_K1520011/annotation2384962/range2384962/1
component: BBa_K1520500

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type: Range
persistent identity: range2384962
display id: range2384962
version: 1
direction: direction/reverse
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start: 64
end: 795
orientation: inline

BBa_K1520029
type: SequenceAnnotation
persistent identity: annotation2384966
display id: annotation2384966
version: 1
top level: BBa_K1520011
location: https://synbiohub.org/public/igem/BBa_K1520011/annotation2384966/range2384966/1
component: BBa_K1520029

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type: Range
persistent identity: range2384966
display id: range2384966
version: 1
direction: direction/reverse
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start: 1473
end: 1601
orientation: inline

BBa_R0062
type: SequenceAnnotation
persistent identity: annotation2384957
display id: annotation2384957
version: 1
top level: BBa_K1520011
location: https://synbiohub.org/public/igem/BBa_K1520011/annotation2384957/range2384957/1
component: BBa_R0062

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type: Range
persistent identity: range2384957
display id: range2384957
version: 1
direction: direction/forward
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start: 1
end: 55
orientation: inline

BBa_K1520504
type: SequenceAnnotation
persistent identity: annotation2384965
display id: annotation2384965
version: 1
top level: BBa_K1520011
location: https://synbiohub.org/public/igem/BBa_K1520011/annotation2384965/range2384965/1
component: BBa_K1520504

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type: Range
persistent identity: range2384965
display id: range2384965
version: 1
direction: direction/reverse
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start: 804
end: 1464
orientation: inline

BBa_K546000
type: ComponentDefinition
persistent identity: BBa K546000
display id: BBa_K546000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Lux pL controlled LuxR with lux pR autoinducing LuxI(lva tag)- AHL.
creator: M. Hesselman
created: 2011-09-13T11:00:00Z
modified: 2015-05-08T01:12:39Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _713_
m user id: 0
owner id: 9055
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: The F2621 BioBrick device contains a lux pL regulated luxR repressor/activator and a lux pR promoter that is linked to an 3OC6HSL, an AHL autoinducer molecule, synthetase. The lux pL promoter yields a weak constitutive downstream expression. When LuxR and 3OC6HSL are produced, the lux pL promoter, however, will be repressed by a complex of the two. Complementary to the lux pL promoter, the lux pR promoter with a complex of luxR and 3OC6HSL will enable enlarged activation of the downstream synthetase gene, thus increase the concentration 3OC6HSL.
mutable notes: The original R0063 BioBrick part (promoter) of the F2621 BioBrick device turned out to be absent in our construct, as was shown by a sequence analysis. To increase the transcription activation there has been put an extra R0063 BioBrick part promoter in front of the F2621 BioBrick device. The resulting construct worked a lot better than the original F2621 - K082014 BioBrick system.
mutable provenance: The lux p promoters and the autoinducer producing enzyme originate from the quorum sensing ability of ''Vibrio fischeri''.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Signalling
role: SO:0000804
component: BBa_F2621
component: BBa_K082014
sequence annotation: BBa_K082014
sequence annotation: BBa_F2621
sequence: https://synbiohub.org/public/igem/BBa_K546000_sequence/1

BBa_F2621
type: Component
persistent identity: component2289549
display id: component2289549
version: 1
top level: BBa_K546000
access: public
definition: https://synbiohub.org/public/igem/BBa_F2621/1

BBa_K082014
type: Component
persistent identity: component2289564
display id: component2289564
version: 1
top level: BBa_K546000
access: public
definition: https://synbiohub.org/public/igem/BBa_K082014/1

BBa_K082014
type: SequenceAnnotation
persistent identity: annotation2289564
display id: annotation2289564
version: 1
top level: BBa_K546000
location: https://synbiohub.org/public/igem/BBa_K546000/annotation2289564/range2289564/1
component: BBa_K082014

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type: Range
persistent identity: range2289564
display id: range2289564
version: 1
direction: direction/reverse
top level: BBa_K546000
start: 1167
end: 1964
orientation: inline

BBa_F2621
type: SequenceAnnotation
persistent identity: annotation2289549
display id: annotation2289549
version: 1
top level: BBa_K546000
location: https://synbiohub.org/public/igem/BBa_K546000/annotation2289549/range2289549/1
component: BBa_F2621

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type: Range
persistent identity: range2289549
display id: range2289549
version: 1
direction: direction/reverse
top level: BBa_K546000
start: 1
end: 1158
orientation: inline

BBa_K082007
type: ComponentDefinition
persistent identity: BBa K082007
display id: BBa_K082007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: B0030-C0070
creator: Zhen Xu , Binjie Xu
created: 2008-10-27T12:00:00Z
modified: 2015-05-08T01:08:35Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _228_
m user id: 0
owner id: 1560
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: none
mutable notes: none
mutable provenance: none
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_C0070
component: BBa_B0030
sequence annotation: BBa_B0030
sequence annotation: BBa_C0070
sequence: https://synbiohub.org/public/igem/BBa_K082007_sequence/1

BBa_C0070
type: Component
persistent identity: component2244044
display id: component2244044
version: 1
top level: BBa_K082007
access: public
definition: rhII

BBa_B0030
type: Component
persistent identity: component2244039
display id: component2244039
version: 1
top level: BBa_K082007
access: public
definition: https://synbiohub.org/public/igem/BBa_B0030/1

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation2244039
display id: annotation2244039
version: 1
top level: BBa_K082007
location: https://synbiohub.org/public/igem/BBa_K082007/annotation2244039/range2244039/1
component: BBa_B0030

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type: Range
persistent identity: range2244039
display id: range2244039
version: 1
direction: direction/forward
top level: BBa_K082007
start: 1
end: 15
orientation: inline

BBa_C0070
type: SequenceAnnotation
persistent identity: annotation2244044
display id: annotation2244044
version: 1
top level: BBa_K082007
location: https://synbiohub.org/public/igem/BBa_K082007/annotation2244044/range2244044/1
component: BBa_C0070

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type: Range
persistent identity: range2244044
display id: range2244044
version: 1
direction: direction/reverse
top level: BBa_K082007
start: 22
end: 688
orientation: inline

BBa_K091109
type: ComponentDefinition
persistent identity: BBa K091109
display id: BBa_K091109
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LuxS
creator: Andrew Gordon
created: 2008-06-18T11:00:00Z
modified: 2015-05-08T01:08:37Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _191_
m user id: 0
owner id: 3129
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: LuxS is a synthase for the AI-2 quorum sensing system. This protein is involved in the production of small molecules that are readily absorbed by multiple species of bacteria. LuxS can be used as part of a positive feedback loop in cell to cell signaling.
mutable notes: Nucleotide position 54 changed from A to T in order to destroy a PstI site; this is a sense mutation Also changed stop codon from TAG to TAA (recommended in Registry Help)
mutable provenance: The LuxS sequence was pulled from E. coli MG1655 by predesigned primers.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K091109_sequence/1

BBa_K546005
type: ComponentDefinition
persistent identity: BBa K546005
display id: BBa_K546005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Lux pL controlled LuxR + lux pR autoinducing LuxI (lva tag) + lux pR controlled GFP(lva tag).
creator: Matthijn Hesselman
created: 2011-09-17T11:00:00Z
modified: 2015-05-08T01:12:39Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _713_
m user id: 0
owner id: 8603
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: in progress
mutable notes: in progress
mutable provenance: in progress
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_J04031
component: BBa_R0063
component: BBa_B0012
component: BBa_B0010
component: BBa_C0062
component: BBa_B0034
component: BBa_R0062
component: BBa_C0061
sequence annotation: BBa_R0062
sequence annotation: BBa_J04031
sequence annotation: BBa_B0012
sequence annotation: BBa_R0063
sequence annotation: BBa_B0010
sequence annotation: BBa_B0034
sequence annotation: BBa_C0061
sequence annotation: BBa_C0062
sequence: https://synbiohub.org/public/igem/BBa_K546005_sequence/1

BBa_J04031
type: Component
persistent identity: component2320957
display id: component2320957
version: 1
top level: BBa_K546005
access: public
definition: https://synbiohub.org/public/igem/BBa_J04031/1

BBa_R0063
type: Component
persistent identity: component2320927
display id: component2320927
version: 1
top level: BBa_K546005
access: public
definition: https://synbiohub.org/public/igem/BBa_R0063/1

BBa_B0012
type: Component
persistent identity: component2320960
display id: component2320960
version: 1
top level: BBa_K546005
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0010
type: Component
persistent identity: component2320978
display id: component2320978
version: 1
top level: BBa_K546005
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_C0062
type: Component
persistent identity: component2320936
display id: component2320936
version: 1
top level: BBa_K546005
access: public
definition: https://synbiohub.org/public/igem/BBa_C0062/1

BBa_B0034
type: Component
persistent identity: component2320990
display id: component2320990
version: 1
top level: BBa_K546005
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0010
type: Component
persistent identity: component2320958
display id: component2320958
version: 1
top level: BBa_K546005
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
type: Component
persistent identity: component2320997
display id: component2320997
version: 1
top level: BBa_K546005
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0034
type: Component
persistent identity: component2320971
display id: component2320971
version: 1
top level: BBa_K546005
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0034
type: Component
persistent identity: component2320933
display id: component2320933
version: 1
top level: BBa_K546005
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0012
type: Component
persistent identity: component2320942
display id: component2320942
version: 1
top level: BBa_K546005
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_R0062
type: Component
persistent identity: component2320947
display id: component2320947
version: 1
top level: BBa_K546005
access: public
definition: https://synbiohub.org/public/igem/BBa_R0062/1

BBa_B0010
type: Component
persistent identity: component2320995
display id: component2320995
version: 1
top level: BBa_K546005
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_R0062
type: Component
persistent identity: component2320985
display id: component2320985
version: 1
top level: BBa_K546005
access: public
definition: https://synbiohub.org/public/igem/BBa_R0062/1

BBa_B0012
type: Component
persistent identity: component2320980
display id: component2320980
version: 1
top level: BBa_K546005
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_C0061
type: Component
persistent identity: component2320992
display id: component2320992
version: 1
top level: BBa_K546005
access: public
definition: luxI

BBa_B0010
type: Component
persistent identity: component2320940
display id: component2320940
version: 1
top level: BBa_K546005
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_R0063
type: Component
persistent identity: component2320965
display id: component2320965
version: 1
top level: BBa_K546005
access: public
definition: https://synbiohub.org/public/igem/BBa_R0063/1

BBa_C0062
type: Component
persistent identity: component2320974
display id: component2320974
version: 1
top level: BBa_K546005
access: public
definition: https://synbiohub.org/public/igem/BBa_C0062/1

BBa_B0034
type: Component
persistent identity: component2320952
display id: component2320952
version: 1
top level: BBa_K546005
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_R0062
type: SequenceAnnotation
persistent identity: annotation2320985
display id: annotation2320985
version: 1
top level: BBa_K546005
location: https://synbiohub.org/public/igem/BBa_K546005/annotation2320985/range2320985/1
component: BBa_R0062

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type: Range
persistent identity: range2320985
display id: range2320985
version: 1
direction: direction/forward
top level: BBa_K546005
start: 3192
end: 3246
orientation: inline

BBa_J04031
type: SequenceAnnotation
persistent identity: annotation2320957
display id: annotation2320957
version: 1
top level: BBa_K546005
location: https://synbiohub.org/public/igem/BBa_K546005/annotation2320957/range2320957/1
component: BBa_J04031

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type: Range
persistent identity: range2320957
display id: range2320957
version: 1
direction: direction/reverse
top level: BBa_K546005
start: 1185
end: 1943
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2320980
display id: annotation2320980
version: 1
top level: BBa_K546005
location: https://synbiohub.org/public/igem/BBa_K546005/annotation2320980/range2320980/1
component: BBa_B0012

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type: Range
persistent identity: range2320980
display id: range2320980
version: 1
direction: direction/forward
top level: BBa_K546005
start: 3143
end: 3183
orientation: inline

BBa_R0063
type: SequenceAnnotation
persistent identity: annotation2320927
display id: annotation2320927
version: 1
top level: BBa_K546005
location: https://synbiohub.org/public/igem/BBa_K546005/annotation2320927/range2320927/1
component: BBa_R0063

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type: Range
persistent identity: range2320927
display id: range2320927
version: 1
direction: direction/forward
top level: BBa_K546005
start: 1
end: 151
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2320978
display id: annotation2320978
version: 1
top level: BBa_K546005
location: https://synbiohub.org/public/igem/BBa_K546005/annotation2320978/range2320978/1
component: BBa_B0010

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type: Range
persistent identity: range2320978
display id: range2320978
version: 1
direction: direction/forward
top level: BBa_K546005
start: 3055
end: 3134
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2320997
display id: annotation2320997
version: 1
top level: BBa_K546005
location: https://synbiohub.org/public/igem/BBa_K546005/annotation2320997/range2320997/1
component: BBa_B0012

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type: Range
persistent identity: range2320997
display id: range2320997
version: 1
direction: direction/forward
top level: BBa_K546005
start: 4012
end: 4052
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2320952
display id: annotation2320952
version: 1
top level: BBa_K546005
location: https://synbiohub.org/public/igem/BBa_K546005/annotation2320952/range2320952/1
component: BBa_B0034

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type: Range
persistent identity: range2320952
display id: range2320952
version: 1
direction: direction/reverse
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start: 1167
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BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2320958
display id: annotation2320958
version: 1
top level: BBa_K546005
location: https://synbiohub.org/public/igem/BBa_K546005/annotation2320958/range2320958/1
component: BBa_B0010

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type: Range
persistent identity: range2320958
display id: range2320958
version: 1
direction: direction/forward
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start: 1952
end: 2031
orientation: inline

BBa_C0061
type: SequenceAnnotation
persistent identity: annotation2320992
display id: annotation2320992
version: 1
top level: BBa_K546005
location: https://synbiohub.org/public/igem/BBa_K546005/annotation2320992/range2320992/1
component: BBa_C0061

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persistent identity: range2320992
display id: range2320992
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direction: direction/forward
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end: 3890
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2320940
display id: annotation2320940
version: 1
top level: BBa_K546005
location: https://synbiohub.org/public/igem/BBa_K546005/annotation2320940/range2320940/1
component: BBa_B0010

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type: Range
persistent identity: range2320940
display id: range2320940
version: 1
direction: direction/forward
top level: BBa_K546005
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orientation: inline

BBa_C0062
type: SequenceAnnotation
persistent identity: annotation2320936
display id: annotation2320936
version: 1
top level: BBa_K546005
location: https://synbiohub.org/public/igem/BBa_K546005/annotation2320936/range2320936/1
component: BBa_C0062

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type: Range
persistent identity: range2320936
display id: range2320936
version: 1
direction: direction/forward
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start: 178
end: 933
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2320933
display id: annotation2320933
version: 1
top level: BBa_K546005
location: https://synbiohub.org/public/igem/BBa_K546005/annotation2320933/range2320933/1
component: BBa_B0034

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type: Range
persistent identity: range2320933
display id: range2320933
version: 1
direction: direction/reverse
top level: BBa_K546005
start: 160
end: 171
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2320971
display id: annotation2320971
version: 1
top level: BBa_K546005
location: https://synbiohub.org/public/igem/BBa_K546005/annotation2320971/range2320971/1
component: BBa_B0034

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type: Range
persistent identity: range2320971
display id: range2320971
version: 1
direction: direction/reverse
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start: 2248
end: 2259
orientation: inline

BBa_R0062
type: SequenceAnnotation
persistent identity: annotation2320947
display id: annotation2320947
version: 1
top level: BBa_K546005
location: https://synbiohub.org/public/igem/BBa_K546005/annotation2320947/range2320947/1
component: BBa_R0062

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type: Range
persistent identity: range2320947
display id: range2320947
version: 1
direction: direction/forward
top level: BBa_K546005
start: 1104
end: 1158
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2320942
display id: annotation2320942
version: 1
top level: BBa_K546005
location: https://synbiohub.org/public/igem/BBa_K546005/annotation2320942/range2320942/1
component: BBa_B0012

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type: Range
persistent identity: range2320942
display id: range2320942
version: 1
direction: direction/forward
top level: BBa_K546005
start: 1055
end: 1095
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2320995
display id: annotation2320995
version: 1
top level: BBa_K546005
location: https://synbiohub.org/public/igem/BBa_K546005/annotation2320995/range2320995/1
component: BBa_B0010

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type: Range
persistent identity: range2320995
display id: range2320995
version: 1
direction: direction/forward
top level: BBa_K546005
start: 3924
end: 4003
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2320990
display id: annotation2320990
version: 1
top level: BBa_K546005
location: https://synbiohub.org/public/igem/BBa_K546005/annotation2320990/range2320990/1
component: BBa_B0034

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type: Range
persistent identity: range2320990
display id: range2320990
version: 1
direction: direction/reverse
top level: BBa_K546005
start: 3255
end: 3266
orientation: inline

BBa_R0063
type: SequenceAnnotation
persistent identity: annotation2320965
display id: annotation2320965
version: 1
top level: BBa_K546005
location: https://synbiohub.org/public/igem/BBa_K546005/annotation2320965/range2320965/1
component: BBa_R0063

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type: Range
persistent identity: range2320965
display id: range2320965
version: 1
direction: direction/forward
top level: BBa_K546005
start: 2089
end: 2239
orientation: inline

BBa_C0062
type: SequenceAnnotation
persistent identity: annotation2320974
display id: annotation2320974
version: 1
top level: BBa_K546005
location: https://synbiohub.org/public/igem/BBa_K546005/annotation2320974/range2320974/1
component: BBa_C0062

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type: Range
persistent identity: range2320974
display id: range2320974
version: 1
direction: direction/forward
top level: BBa_K546005
start: 2266
end: 3021
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2320960
display id: annotation2320960
version: 1
top level: BBa_K546005
location: https://synbiohub.org/public/igem/BBa_K546005/annotation2320960/range2320960/1
component: BBa_B0012

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type: Range
persistent identity: range2320960
display id: range2320960
version: 1
direction: direction/forward
top level: BBa_K546005
start: 2040
end: 2080
orientation: inline

BBa_K1520510
type: ComponentDefinition
persistent identity: BBa K1520510
display id: BBa_K1520510
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PgolTS-golS-PgolB-rbs-luxI-Ter-Pcons2-rbs-luxR-Ter-Prlux-rbs-OMPA-golB-rbs-luxI-Ter
creator: yue wu
created: 2014-09-22T11:00:00Z
modified: 2015-05-08T01:10:49Z
discontinued: false
dominant: false
group u list: _1902_
m user id: 0
owner id: 21318
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: PgolTS-golS-PgolB-rbs-luxI-Ter-Pcons2-rbs-luxR-Ter-Prlux-rbs-OMPA-golB-rbs-luxI-Ter It is a test of our positive feedback circuit for gold ions.
mutable notes: None
mutable provenance: PgolTS-golS-PgolB, luxI, Pcons2, luxR, Prlux, OMPA-golB, Ter
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1520029
component: BBa_K1520506
component: BBa_K1520003
component: BBa_K1520504
component: BBa_K1520507
component: BBa_R0062
sequence annotation: BBa_K1520507
sequence annotation: BBa_R0062
sequence annotation: BBa_K1520003
sequence annotation: BBa_K1520029
sequence annotation: BBa_K1520506
sequence annotation: BBa_K1520504
sequence: https://synbiohub.org/public/igem/BBa_K1520510_sequence/1

BBa_K1520029
type: Component
persistent identity: component2385589
display id: component2385589
version: 1
top level: BBa_K1520510
access: public
definition: https://synbiohub.org/public/igem/BBa_K1520029/1

BBa_K1520506
type: Component
persistent identity: component2385563
display id: component2385563
version: 1
top level: BBa_K1520510
access: public
definition: https://synbiohub.org/public/igem/BBa_K1520506/1

BBa_K1520029
type: Component
persistent identity: component2385567
display id: component2385567
version: 1
top level: BBa_K1520510
access: public
definition: https://synbiohub.org/public/igem/BBa_K1520029/1

BBa_K1520003
type: Component
persistent identity: component2385573
display id: component2385573
version: 1
top level: BBa_K1520510
access: public
definition: https://synbiohub.org/public/igem/BBa_K1520003/1

BBa_K1520504
type: Component
persistent identity: component2385566
display id: component2385566
version: 1
top level: BBa_K1520510
access: public
definition: BBa_K1520504

BBa_K1520504
type: Component
persistent identity: component2385588
display id: component2385588
version: 1
top level: BBa_K1520510
access: public
definition: BBa_K1520504

BBa_K1520507
type: Component
persistent identity: component2385585
display id: component2385585
version: 1
top level: BBa_K1520510
access: public
definition: https://synbiohub.org/public/igem/BBa_K1520507/1

BBa_R0062
type: Component
persistent identity: component2385575
display id: component2385575
version: 1
top level: BBa_K1520510
access: public
definition: https://synbiohub.org/public/igem/BBa_R0062/1

BBa_K1520507
type: SequenceAnnotation
persistent identity: annotation2385585
display id: annotation2385585
version: 1
top level: BBa_K1520510
location: https://synbiohub.org/public/igem/BBa_K1520510/annotation2385585/range2385585/1
component: BBa_K1520507

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type: Range
persistent identity: range2385585
display id: range2385585
version: 1
direction: direction/reverse
top level: BBa_K1520510
start: 2616
end: 3425
orientation: inline

BBa_R0062
type: SequenceAnnotation
persistent identity: annotation2385575
display id: annotation2385575
version: 1
top level: BBa_K1520510
location: https://synbiohub.org/public/igem/BBa_K1520510/annotation2385575/range2385575/1
component: BBa_R0062

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type: Range
persistent identity: range2385575
display id: range2385575
version: 1
direction: direction/forward
top level: BBa_K1520510
start: 2555
end: 2609
orientation: inline

BBa_K1520003
type: SequenceAnnotation
persistent identity: annotation2385573
display id: annotation2385573
version: 1
top level: BBa_K1520510
location: https://synbiohub.org/public/igem/BBa_K1520510/annotation2385573/range2385573/1
component: BBa_K1520003

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type: Range
persistent identity: range2385573
display id: range2385573
version: 1
direction: direction/reverse
top level: BBa_K1520510
start: 1568
end: 2546
orientation: inline

BBa_K1520029
type: SequenceAnnotation
persistent identity: annotation2385567
display id: annotation2385567
version: 1
top level: BBa_K1520510
location: https://synbiohub.org/public/igem/BBa_K1520510/annotation2385567/range2385567/1
component: BBa_K1520029

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type: Range
persistent identity: range2385567
display id: range2385567
version: 1
direction: direction/reverse
top level: BBa_K1520510
start: 1431
end: 1559
orientation: inline

BBa_K1520506
type: SequenceAnnotation
persistent identity: annotation2385563
display id: annotation2385563
version: 1
top level: BBa_K1520510
location: https://synbiohub.org/public/igem/BBa_K1520510/annotation2385563/range2385563/1
component: BBa_K1520506

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type: Range
persistent identity: range2385563
display id: range2385563
version: 1
direction: direction/reverse
top level: BBa_K1520510
start: 1
end: 753
orientation: inline

BBa_K1520504
type: SequenceAnnotation
persistent identity: annotation2385566
display id: annotation2385566
version: 1
top level: BBa_K1520510
location: https://synbiohub.org/public/igem/BBa_K1520510/annotation2385566/range2385566/1
component: BBa_K1520504

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type: Range
persistent identity: range2385566
display id: range2385566
version: 1
direction: direction/reverse
top level: BBa_K1520510
start: 762
end: 1422
orientation: inline

BBa_K1520029
type: SequenceAnnotation
persistent identity: annotation2385589
display id: annotation2385589
version: 1
top level: BBa_K1520510
location: https://synbiohub.org/public/igem/BBa_K1520510/annotation2385589/range2385589/1
component: BBa_K1520029

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type: Range
persistent identity: range2385589
display id: range2385589
version: 1
direction: direction/reverse
top level: BBa_K1520510
start: 4103
end: 4231
orientation: inline

BBa_K1520504
type: SequenceAnnotation
persistent identity: annotation2385588
display id: annotation2385588
version: 1
top level: BBa_K1520510
location: https://synbiohub.org/public/igem/BBa_K1520510/annotation2385588/range2385588/1
component: BBa_K1520504

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type: Range
persistent identity: range2385588
display id: range2385588
version: 1
direction: direction/reverse
top level: BBa_K1520510
start: 3434
end: 4094
orientation: inline

rhII
type: ComponentDefinition
persistent identity: BBa C0070
display id: BBa_C0070
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: autoinducer synthetase for N-butyryl-HSL (BHL) and HHL
creator: Debra Lin, Srini Devadas, David Gray, Ronny Krashinsky, and Chris Zheng Liu, Boopers
created: 2004-01-26T12:00:00Z
modified: 2015-08-31T04:07:23Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Autoinducer synthesis protein that produces N-butyryl-HSL which binds to RhlR, obtained from Pseudomonas aeruginosa.
mutable notes: Editing Check (1/28/04) (0) BB sites cleaned (1) ATG (2) TAATAA (3) Blast checks out (i.e., it's RhlI) (4) Codon changes OK (AA and tRNA usage) (5) Chassis might have conflict (w/ native autoinducer system?) (6) ssrA tag added (7) barcode added (8) Codon optimized for expression in enteric bacteria 2 silent point mutations have been included into the sequence at base 138 from A to G and at base 576 from G to C to remove internal EcoRI and PstI sites. Mutations were chosen to yield the same amino acid and codons of similar frequency in E. coli.
mutable provenance: P. aeruginosa PA3476
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Help:Barcodes
sequence annotation: rhlL
sequence annotation: LVA
sequence: https://synbiohub.org/public/igem/BBa_C0070_sequence/1

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213996
display id: annotation2213996
version: 1
top level: rhII
role: SO:0000807
role: feature/barcode
location: https://synbiohub.org/public/igem/BBa_C0070/annotation2213996/range2213996/1

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type: Range
persistent identity: range2213996
display id: range2213996
version: 1
direction: direction/forward
top level: rhII
start: 643
end: 667
orientation: inline

rhlL
type: SequenceAnnotation
persistent identity: annotation301203
display id: annotation301203
version: 1
top level: rhII
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_C0070/annotation301203/range301203/1

https://synbiohub.org/public/igem/BBa_C0070/annotation301203/range301203/1
type: Range
persistent identity: range301203
display id: range301203
version: 1
direction: direction/forward
top level: rhII
start: 1
end: 603
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation306820
display id: annotation306820
version: 1
top level: rhII
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_C0070/annotation306820/range306820/1

https://synbiohub.org/public/igem/BBa_C0070/annotation306820/range306820/1
type: Range
persistent identity: range306820
display id: range306820
version: 1
direction: direction/forward
top level: rhII
start: 604
end: 636
orientation: inline

BBa_C0261
type: ComponentDefinition
persistent identity: BBa C0261
display id: BBa_C0261
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: AHL-making Enzyme, luxI (+RBS)
creator: robertb
created: 2004-08-22T11:00:00Z
modified: 2015-08-31T04:07:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _6_
m user id: 0
owner id: 159
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: B0034.C0061
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_C0061
component: BBa_B0034
sequence annotation: BBa_B0034
sequence annotation: BBa_C0061
sequence: https://synbiohub.org/public/igem/BBa_C0261_sequence/1

BBa_C0061
type: Component
persistent identity: component1060413
display id: component1060413
version: 1
top level: BBa_C0261
access: public
definition: luxI

BBa_B0034
type: Component
persistent identity: component1060403
display id: component1060403
version: 1
top level: BBa_C0261
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1060403
display id: annotation1060403
version: 1
top level: BBa_C0261
location: https://synbiohub.org/public/igem/BBa_C0261/annotation1060403/range1060403/1
component: BBa_B0034

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type: Range
persistent identity: range1060403
display id: range1060403
version: 1
direction: direction/forward
top level: BBa_C0261
start: 1
end: 12
orientation: inline

BBa_C0061
type: SequenceAnnotation
persistent identity: annotation1060413
display id: annotation1060413
version: 1
top level: BBa_C0261
location: https://synbiohub.org/public/igem/BBa_C0261/annotation1060413/range1060413/1
component: BBa_C0061

https://synbiohub.org/public/igem/BBa_C0261/annotation1060413/range1060413/1
type: Range
persistent identity: range1060413
display id: range1060413
version: 1
direction: direction/forward
top level: BBa_C0261
start: 19
end: 636
orientation: inline

luxI
type: ComponentDefinition
persistent identity: BBa C0061
display id: BBa_C0061
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: autoinducer synthetase for AHL
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:23Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Synthesizes 3OC6HSL, which binds to LuxR. The lux cassette of V. fischeri contains a left and a right promoter. The right promoter gives weak constitutive expression of downstream genes.This expression is up-regulated by the action of the Lux repressor, LuxR. Two molecules of LuxR protein form a complex with two molecules the signalling compound HSL. This complex binds to a palindromic site on the promoter, increasing the rate of transcription.
mutable notes: An LVA tail (sequence: AANDENYALVA) was added to increase protein degradation. .
mutable provenance: V. fischeri <genbank>AF170104</genbank>
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: luxI
sequence annotation: LVA
sequence annotation: BBa_C0061
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0061_sequence/1

luxI
type: SequenceAnnotation
persistent identity: annotation1761
display id: annotation1761
version: 1
top level: luxI
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_C0061/annotation1761/range1761/1

https://synbiohub.org/public/igem/BBa_C0061/annotation1761/range1761/1
type: Range
persistent identity: range1761
display id: range1761
version: 1
direction: direction/forward
top level: luxI
start: 1
end: 579
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation1760
display id: annotation1760
version: 1
top level: luxI
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_C0061/annotation1760/range1760/1

https://synbiohub.org/public/igem/BBa_C0061/annotation1760/range1760/1
type: Range
persistent identity: range1760
display id: range1760
version: 1
direction: direction/forward
top level: luxI
start: 580
end: 611
orientation: inline

BBa_C0061
type: SequenceAnnotation
persistent identity: annotation7038
display id: annotation7038
version: 1
top level: luxI
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_C0061/annotation7038/range7038/1

https://synbiohub.org/public/igem/BBa_C0061/annotation7038/range7038/1
type: Range
persistent identity: range7038
display id: range7038
version: 1
direction: direction/forward
top level: luxI
start: 1
end: 618
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213985
display id: annotation2213985
version: 1
top level: luxI
role: SO:0000807
role: feature/barcode
location: https://synbiohub.org/public/igem/BBa_C0061/annotation2213985/range2213985/1

https://synbiohub.org/public/igem/BBa_C0061/annotation2213985/range2213985/1
type: Range
persistent identity: range2213985
display id: range2213985
version: 1
direction: direction/forward
top level: luxI
start: 619
end: 643
orientation: inline

BBa_K1520022
type: ComponentDefinition
persistent identity: BBa K1520022
display id: BBa_K1520022
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Pcons2-rbs-lacI-Ter-Plac-rbs-luxI-Ter-Pcons2-rbs-luxR-Ter-Prlux-rbs-rfp-rbs-luxI-Ter
creator: yue wu
created: 2014-09-21T11:00:00Z
modified: 2015-05-08T01:10:49Z
discontinued: false
dominant: false
group u list: _1902_
m user id: 0
owner id: 21318
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Plasmid in positive feedback verification: Pcons2-rbs-lacI-Ter-Plac-rbs-luxI-Ter-Pcons2-rbs-luxR-Ter-Prlux-rbs-rfp-rbs-luxI-Ter It is a positive feedback plasmid using luxI as key to feedback. Introducing IPTG will produce rfp and luxI, and luxI will work back to enhance production of rfp.
mutable notes: None
mutable provenance: Pcons2, lacI, Plac, luxI, luxR, Prlux, rfp, Ter
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1520011
component: BBa_K1520020
sequence annotation: BBa_K1520011
sequence annotation: BBa_K1520020
sequence: https://synbiohub.org/public/igem/BBa_K1520022_sequence/1

BBa_K1520011
type: Component
persistent identity: component2385187
display id: component2385187
version: 1
top level: BBa_K1520022
access: public
definition: BBa_K1520011

BBa_K1520020
type: Component
persistent identity: component2385176
display id: component2385176
version: 1
top level: BBa_K1520022
access: public
definition: https://synbiohub.org/public/igem/BBa_K1520020/1

BBa_K1520011
type: SequenceAnnotation
persistent identity: annotation2385187
display id: annotation2385187
version: 1
top level: BBa_K1520022
location: https://synbiohub.org/public/igem/BBa_K1520022/annotation2385187/range2385187/1
component: BBa_K1520011

https://synbiohub.org/public/igem/BBa_K1520022/annotation2385187/range2385187/1
type: Range
persistent identity: range2385187
display id: range2385187
version: 1
direction: direction/reverse
top level: BBa_K1520022
start: 3361
end: 4961
orientation: inline

BBa_K1520020
type: SequenceAnnotation
persistent identity: annotation2385176
display id: annotation2385176
version: 1
top level: BBa_K1520022
location: https://synbiohub.org/public/igem/BBa_K1520022/annotation2385176/range2385176/1
component: BBa_K1520020

https://synbiohub.org/public/igem/BBa_K1520022/annotation2385176/range2385176/1
type: Range
persistent identity: range2385176
display id: range2385176
version: 1
direction: direction/reverse
top level: BBa_K1520022
start: 1
end: 3352
orientation: inline

lasI
type: ComponentDefinition
persistent identity: BBa C0178
display id: BBa_C0178
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: autoinducer synthetase for PAI from Pseudomonas aeruginosa (no LVA)
creator: jcbraff
created: 2004-05-26T11:00:00Z
modified: 2015-08-31T04:07:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_1_
m user id: 0
owner id: 61
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: same as C0078 except no LVA tag
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: lasI
sequence: https://synbiohub.org/public/igem/BBa_C0178_sequence/1

lasI
type: SequenceAnnotation
persistent identity: annotation1937124
display id: annotation1937124
version: 1
top level: lasI
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_C0178/annotation1937124/range1937124/1

https://synbiohub.org/public/igem/BBa_C0178/annotation1937124/range1937124/1
type: Range
persistent identity: range1937124
display id: range1937124
version: 1
direction: direction/forward
top level: lasI
start: 1
end: 609
orientation: inline

BBa_K1520508
type: ComponentDefinition
persistent identity: BBa K1520508
display id: BBa_K1520508
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PgolTS-golS-PgolB-rbs-luxI-Ter
creator: yue wu
created: 2014-09-22T11:00:00Z
modified: 2015-05-08T01:10:49Z
discontinued: false
dominant: false
group u list: _1902_
m user id: 0
owner id: 21318
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: PgolTS-golS-PgolB-rbs-luxI-Ter If there are gold ions, the luxI will be produced.
mutable notes: None
mutable provenance: PgolTS-golS-PgolB, luxI, Ter
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1520506
component: BBa_K1520504
component: BBa_K1520029
sequence annotation: BBa_K1520506
sequence annotation: BBa_K1520504
sequence annotation: BBa_K1520029
sequence: https://synbiohub.org/public/igem/BBa_K1520508_sequence/1

BBa_K1520506
type: Component
persistent identity: component2385519
display id: component2385519
version: 1
top level: BBa_K1520508
access: public
definition: https://synbiohub.org/public/igem/BBa_K1520506/1

BBa_K1520504
type: Component
persistent identity: component2385522
display id: component2385522
version: 1
top level: BBa_K1520508
access: public
definition: BBa_K1520504

BBa_K1520029
type: Component
persistent identity: component2385523
display id: component2385523
version: 1
top level: BBa_K1520508
access: public
definition: https://synbiohub.org/public/igem/BBa_K1520029/1

BBa_K1520506
type: SequenceAnnotation
persistent identity: annotation2385519
display id: annotation2385519
version: 1
top level: BBa_K1520508
location: https://synbiohub.org/public/igem/BBa_K1520508/annotation2385519/range2385519/1
component: BBa_K1520506

https://synbiohub.org/public/igem/BBa_K1520508/annotation2385519/range2385519/1
type: Range
persistent identity: range2385519
display id: range2385519
version: 1
direction: direction/reverse
top level: BBa_K1520508
start: 1
end: 753
orientation: inline

BBa_K1520504
type: SequenceAnnotation
persistent identity: annotation2385522
display id: annotation2385522
version: 1
top level: BBa_K1520508
location: https://synbiohub.org/public/igem/BBa_K1520508/annotation2385522/range2385522/1
component: BBa_K1520504

https://synbiohub.org/public/igem/BBa_K1520508/annotation2385522/range2385522/1
type: Range
persistent identity: range2385522
display id: range2385522
version: 1
direction: direction/reverse
top level: BBa_K1520508
start: 762
end: 1422
orientation: inline

BBa_K1520029
type: SequenceAnnotation
persistent identity: annotation2385523
display id: annotation2385523
version: 1
top level: BBa_K1520508
location: https://synbiohub.org/public/igem/BBa_K1520508/annotation2385523/range2385523/1
component: BBa_K1520029

https://synbiohub.org/public/igem/BBa_K1520508/annotation2385523/range2385523/1
type: Range
persistent identity: range2385523
display id: range2385523
version: 1
direction: direction/reverse
top level: BBa_K1520508
start: 1431
end: 1559
orientation: inline

luxI
type: ComponentDefinition
persistent identity: BBa C0161
display id: BBa_C0161
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: autoinducer synthetase for AHL (no LVA)
creator: jcbraff
created: 2004-05-26T11:00:00Z
modified: 2015-08-31T04:07:24Z
discontinued: false
dominant: true
experience: experience/None
group u list: _11_1_
m user id: 0
owner id: 61
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: same as C0061 except no LVA tag
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: LuxI
sequence: https://synbiohub.org/public/igem/BBa_C0161_sequence/1

LuxI
type: SequenceAnnotation
persistent identity: annotation1891615
display id: annotation1891615
version: 1
top level: luxI
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_C0161/annotation1891615/range1891615/1

https://synbiohub.org/public/igem/BBa_C0161/annotation1891615/range1891615/1
type: Range
persistent identity: range1891615
display id: range1891615
version: 1
direction: direction/forward
top level: luxI
start: 1
end: 585
orientation: inline

BBa_I722012
type: ComponentDefinition
persistent identity: BBa I722012
display id: BBa_I722012
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: AHL->RoPs and mRFP producer
creator: Bo Hu
created: 2007-10-21T11:00:00Z
modified: 2015-08-31T04:07:53Z
discontinued: false
dominant: false
group u list: _150_
m user id: 0
owner id: 1325
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: accept AHL signal to produce RoPS and could also produce mRFP
mutable notes: could be easily connect to a protein coding region with a terminator
mutable provenance: ligation of original part
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0034
component: BBa_I722011
sequence annotation: BBa_I722011
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_I722012_sequence/1

BBa_B0034
type: Component
persistent identity: component2301823
display id: component2301823
version: 1
top level: BBa_I722012
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_I722011
type: Component
persistent identity: component2301821
display id: component2301821
version: 1
top level: BBa_I722012
access: public
definition: BBa_I722011

BBa_I722011
type: SequenceAnnotation
persistent identity: annotation2301821
display id: annotation2301821
version: 1
top level: BBa_I722012
location: https://synbiohub.org/public/igem/BBa_I722012/annotation2301821/range2301821/1
component: BBa_I722011

https://synbiohub.org/public/igem/BBa_I722012/annotation2301821/range2301821/1
type: Range
persistent identity: range2301821
display id: range2301821
version: 1
direction: direction/reverse
top level: BBa_I722012
start: 1
end: 2702
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2301823
display id: annotation2301823
version: 1
top level: BBa_I722012
location: https://synbiohub.org/public/igem/BBa_I722012/annotation2301823/range2301823/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I722012/annotation2301823/range2301823/1
type: Range
persistent identity: range2301823
display id: range2301823
version: 1
direction: direction/reverse
top level: BBa_I722012
start: 2711
end: 2722
orientation: inline

BBa_K1342001
type: ComponentDefinition
persistent identity: BBa K1342001
display id: BBa_K1342001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: zinTp_AspA
creator: Naoki Saito,Daiki Haraguchi,Yoshiharu Otaki,Ryuhei Minei
created: 2014-10-02T11:00:00Z
modified: 2015-05-08T01:09:59Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1717_
m user id: 0
owner id: 22434
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This is
mutable notes: soon
mutable provenance: it come from
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
sequence annotation: cds
sequence annotation: promoter
sequence annotation: rbs
sequence: https://synbiohub.org/public/igem/BBa_K1342001_sequence/1

cds
type: SequenceAnnotation
persistent identity: annotation2394012
display id: annotation2394012
version: 1
top level: BBa_K1342001
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1342001/annotation2394012/range2394012/1

https://synbiohub.org/public/igem/BBa_K1342001/annotation2394012/range2394012/1
type: Range
persistent identity: range2394012
display id: range2394012
version: 1
direction: direction/forward
top level: BBa_K1342001
start: 108
end: 1589
orientation: inline

promoter
type: SequenceAnnotation
persistent identity: annotation2394010
display id: annotation2394010
version: 1
top level: BBa_K1342001
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1342001/annotation2394010/range2394010/1

https://synbiohub.org/public/igem/BBa_K1342001/annotation2394010/range2394010/1
type: Range
persistent identity: range2394010
display id: range2394010
version: 1
direction: direction/forward
top level: BBa_K1342001
start: 1
end: 81
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation2394011
display id: annotation2394011
version: 1
top level: BBa_K1342001
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1342001/annotation2394011/range2394011/1

https://synbiohub.org/public/igem/BBa_K1342001/annotation2394011/range2394011/1
type: Range
persistent identity: range2394011
display id: range2394011
version: 1
direction: direction/forward
top level: BBa_K1342001
start: 82
end: 101
orientation: inline

rhII
type: ComponentDefinition
persistent identity: BBa C0170
display id: BBa_C0170
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: autoinducer synthetase for N-butyryl-HSL (BHL) and HHL (no LVA)
creator: jcbraff
created: 2004-05-26T11:00:00Z
modified: 2015-08-31T04:07:24Z
discontinued: false
dominant: true
experience: experience/None
group u list: _11_1_
m user id: 0
owner id: 61
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: same as C0070 except no LVA tag
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: rhII
sequence: https://synbiohub.org/public/igem/BBa_C0170_sequence/1

rhII
type: SequenceAnnotation
persistent identity: annotation1891586
display id: annotation1891586
version: 1
top level: rhII
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_C0170/annotation1891586/range1891586/1

https://synbiohub.org/public/igem/BBa_C0170/annotation1891586/range1891586/1
type: Range
persistent identity: range1891586
display id: range1891586
version: 1
direction: direction/forward
top level: rhII
start: 1
end: 609
orientation: inline

BBa_K082010
type: ComponentDefinition
persistent identity: BBa K082010
display id: BBa_K082010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: B0034-C0070
creator: Zhen Xu , Binjie Xu
created: 2008-10-27T12:00:00Z
modified: 2015-05-08T01:08:35Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _228_
m user id: 0
owner id: 1560
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: none
mutable notes: none
mutable provenance: none
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_B0034
component: BBa_C0070
sequence annotation: BBa_B0034
sequence annotation: BBa_C0070
sequence: https://synbiohub.org/public/igem/BBa_K082010_sequence/1

BBa_B0034
type: Component
persistent identity: component2244059
display id: component2244059
version: 1
top level: BBa_K082010
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_C0070
type: Component
persistent identity: component2244063
display id: component2244063
version: 1
top level: BBa_K082010
access: public
definition: rhII

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2244059
display id: annotation2244059
version: 1
top level: BBa_K082010
location: https://synbiohub.org/public/igem/BBa_K082010/annotation2244059/range2244059/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K082010/annotation2244059/range2244059/1
type: Range
persistent identity: range2244059
display id: range2244059
version: 1
direction: direction/reverse
top level: BBa_K082010
start: 1
end: 12
orientation: inline

BBa_C0070
type: SequenceAnnotation
persistent identity: annotation2244063
display id: annotation2244063
version: 1
top level: BBa_K082010
location: https://synbiohub.org/public/igem/BBa_K082010/annotation2244063/range2244063/1
component: BBa_C0070

https://synbiohub.org/public/igem/BBa_K082010/annotation2244063/range2244063/1
type: Range
persistent identity: range2244063
display id: range2244063
version: 1
direction: direction/reverse
top level: BBa_K082010
start: 19
end: 685
orientation: inline

cinI
type: ComponentDefinition
persistent identity: BBa C0076
display id: BBa_C0076
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: autoinducer synthetase
creator: crackdots
created: 2004-01-27T12:00:00Z
modified: 2015-08-31T04:07:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: cinI codes for an autoinducer synthetase which utilizes SAM to make O3-C14:1-HSL. In complex with O3-C14:1-HSL, CinR (BBa_C0077) binds to the Cin promoter (BBa_R0077) and activates transcription.
mutable notes: The regulatory locus cinRI in Rhizobium leguminosarum conrols a network of quorum-sensing loci Lithgow, JK; Wilkinson, A; Hardman, A; Rodelas, B; Wisniewski-Dye, F; Williams, P; Downie, AJ MOL. MICROBIOLOGY 37(1): 81-97, 2000 Change log: original STOP: tga -> tAaTAA
mutable provenance: Rhizobium leguminosarum
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Help:Barcodes
sequence annotation: CinI
sequence annotation: LVA
sequence: https://synbiohub.org/public/igem/BBa_C0076_sequence/1

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2214001
display id: annotation2214001
version: 1
top level: cinI
role: SO:0000807
role: feature/barcode
location: https://synbiohub.org/public/igem/BBa_C0076/annotation2214001/range2214001/1

https://synbiohub.org/public/igem/BBa_C0076/annotation2214001/range2214001/1
type: Range
persistent identity: range2214001
display id: range2214001
version: 1
direction: direction/forward
top level: cinI
start: 703
end: 727
orientation: inline

CinI
type: SequenceAnnotation
persistent identity: annotation301035
display id: annotation301035
version: 1
top level: cinI
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_C0076/annotation301035/range301035/1

https://synbiohub.org/public/igem/BBa_C0076/annotation301035/range301035/1
type: Range
persistent identity: range301035
display id: range301035
version: 1
direction: direction/forward
top level: cinI
start: 1
end: 663
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation306586
display id: annotation306586
version: 1
top level: cinI
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_C0076/annotation306586/range306586/1

https://synbiohub.org/public/igem/BBa_C0076/annotation306586/range306586/1
type: Range
persistent identity: range306586
display id: range306586
version: 1
direction: direction/forward
top level: cinI
start: 664
end: 696
orientation: inline

BBa_I722011
type: ComponentDefinition
persistent identity: BBa I722011
display id: BBa_I722011
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: rhl producer followed by a AHL acceptor
creator: Bo Hu
created: 2007-10-20T11:00:00Z
modified: 2015-08-31T04:07:53Z
discontinued: false
dominant: false
experience: experience/None
group u list: _150_
m user id: 0
owner id: 1325
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: this part could accept AHLs and also could produce mRFP easily to detect
mutable notes: ligate the mRFP producer and AHL acceptor together
mutable provenance: Based on the parts provided by MIT
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_I0405
component: BBa_F2620
sequence annotation: BBa_F2620
sequence annotation: BBa_I0405
sequence: https://synbiohub.org/public/igem/BBa_I722011_sequence/1

BBa_I0405
type: Component
persistent identity: component2299180
display id: component2299180
version: 1
top level: BBa_I722011
access: public
definition: https://synbiohub.org/public/igem/BBa_I0405/1

BBa_F2620
type: Component
persistent identity: component2299206
display id: component2299206
version: 1
top level: BBa_I722011
access: public
definition: https://synbiohub.org/public/igem/BBa_F2620/1

BBa_F2620
type: SequenceAnnotation
persistent identity: annotation2299206
display id: annotation2299206
version: 1
top level: BBa_I722011
location: https://synbiohub.org/public/igem/BBa_I722011/annotation2299206/range2299206/1
component: BBa_F2620

https://synbiohub.org/public/igem/BBa_I722011/annotation2299206/range2299206/1
type: Range
persistent identity: range2299206
display id: range2299206
version: 1
direction: direction/reverse
top level: BBa_I722011
start: 1642
end: 2702
orientation: inline

BBa_I0405
type: SequenceAnnotation
persistent identity: annotation2299180
display id: annotation2299180
version: 1
top level: BBa_I722011
location: https://synbiohub.org/public/igem/BBa_I722011/annotation2299180/range2299180/1
component: BBa_I0405

https://synbiohub.org/public/igem/BBa_I722011/annotation2299180/range2299180/1
type: Range
persistent identity: range2299180
display id: range2299180
version: 1
direction: direction/reverse
top level: BBa_I722011
start: 1
end: 1633
orientation: inline

BBa_K082008
type: ComponentDefinition
persistent identity: BBa K082008
display id: BBa_K082008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: B0032-C0070
creator: Zhen Xu , Binjie Xu
created: 2008-10-27T12:00:00Z
modified: 2015-05-08T01:08:35Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _228_
m user id: 0
owner id: 1560
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: none
mutable notes: none
mutable provenance: none
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_B0032
component: BBa_C0070
sequence annotation: BBa_B0032
sequence annotation: BBa_C0070
sequence: https://synbiohub.org/public/igem/BBa_K082008_sequence/1

BBa_B0032
type: Component
persistent identity: component2244046
display id: component2244046
version: 1
top level: BBa_K082008
access: public
definition: https://synbiohub.org/public/igem/BBa_B0032/1

BBa_C0070
type: Component
persistent identity: component2244051
display id: component2244051
version: 1
top level: BBa_K082008
access: public
definition: rhII

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation2244046
display id: annotation2244046
version: 1
top level: BBa_K082008
location: https://synbiohub.org/public/igem/BBa_K082008/annotation2244046/range2244046/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_K082008/annotation2244046/range2244046/1
type: Range
persistent identity: range2244046
display id: range2244046
version: 1
direction: direction/forward
top level: BBa_K082008
start: 1
end: 13
orientation: inline

BBa_C0070
type: SequenceAnnotation
persistent identity: annotation2244051
display id: annotation2244051
version: 1
top level: BBa_K082008
location: https://synbiohub.org/public/igem/BBa_K082008/annotation2244051/range2244051/1
component: BBa_C0070

https://synbiohub.org/public/igem/BBa_K082008/annotation2244051/range2244051/1
type: Range
persistent identity: range2244051
display id: range2244051
version: 1
direction: direction/reverse
top level: BBa_K082008
start: 20
end: 686
orientation: inline