//function/biosynthesis/heme
type: Collection
persistent identity: function biosynthesis heme
display id: function_biosynthesis_heme
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
owned by: james
owned by: myers
member: https://synbiohub.org/public/igem/BBa_I716152/1
member: https://synbiohub.org/public/igem/BBa_K1438000/1
member: https://synbiohub.org/public/igem/BBa_I716154/1
member: https://synbiohub.org/public/igem/BBa_Y00100/1
member: https://synbiohub.org/public/igem/BBa_I716153/1
member: https://synbiohub.org/public/igem/BBa_I716155/1

//function/biosynthesis/plastic
type: Collection
persistent identity: function biosynthesis plastic
display id: function_biosynthesis_plastic
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
owned by: james
owned by: myers
member: https://synbiohub.org/public/igem/BBa_K125504/1
member: https://synbiohub.org/public/igem/BBa_K125801/1
member: https://synbiohub.org/public/igem/BBa_K338024/1
member: https://synbiohub.org/public/igem/BBa_K759004/1
member: https://synbiohub.org/public/igem/BBa_K156016/1
member: https://synbiohub.org/public/igem/BBa_K759009/1
member: https://synbiohub.org/public/igem/BBa_K156033/1
member: https://synbiohub.org/public/igem/BBa_K156012/1
member: https://synbiohub.org/public/igem/BBa_K759011/1
member: https://synbiohub.org/public/igem/BBa_K338023/1
member: https://synbiohub.org/public/igem/BBa_K156021/1
member: https://synbiohub.org/public/igem/BBa_K156031/1
member: https://synbiohub.org/public/igem/BBa_K759007/1
member: https://synbiohub.org/public/igem/BBa_K156013/1
member: https://synbiohub.org/public/igem/BBa_K125501/1
member: https://synbiohub.org/public/igem/BBa_K338022/1
member: https://synbiohub.org/public/igem/BBa_K125802/1
member: https://synbiohub.org/public/igem/BBa_K156022/1
member: https://synbiohub.org/public/igem/BBa_K759006/1
member: https://synbiohub.org/public/igem/BBa_K156018/1
member: https://synbiohub.org/public/igem/BBa_K125502/1
member: https://synbiohub.org/public/igem/BBa_K759010/1
member: https://synbiohub.org/public/igem/BBa_K156014/1
member: https://synbiohub.org/public/igem/BBa_K125803/1
member: https://synbiohub.org/public/igem/BBa_K338004/1
member: https://synbiohub.org/public/igem/BBa_K759005/1
member: https://synbiohub.org/public/igem/BBa_K156015/1
member: https://synbiohub.org/public/igem/BBa_K759008/1
member: https://synbiohub.org/public/igem/BBa_K125804/1
member: https://synbiohub.org/public/igem/BBa_K125503/1
member: https://synbiohub.org/public/igem/BBa_K338003/1

//function/biosynthesis/phycocyanobilin
type: Collection
persistent identity: function biosynthesis phycocyanobilin
display id: function_biosynthesis_phycocyanobilin
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
owned by: james
owned by: myers
member: https://synbiohub.org/public/igem/BBa_I15009/1
member: https://synbiohub.org/public/igem/BBa_K990003/1
member: https://synbiohub.org/public/igem/BBa_K1660004/1
member: https://synbiohub.org/public/igem/BBa_I15008/1

//function/biosynthesis/odorant
type: Collection
persistent identity: function biosynthesis odorant
display id: function_biosynthesis_odorant
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
owned by: james
owned by: myers
member: https://synbiohub.org/public/igem/BBa_J45008/1
member: https://synbiohub.org/public/igem/BBa_J45098/1
member: https://synbiohub.org/public/igem/BBa_J45014/1
member: https://synbiohub.org/public/igem/BBa_J45298/1
member: https://synbiohub.org/public/igem/BBa_I742107/1
member: https://synbiohub.org/public/igem/BBa_K1660003/1
member: https://synbiohub.org/public/igem/BBa_J45001/1
member: https://synbiohub.org/public/igem/BBa_J45002/1
member: https://synbiohub.org/public/igem/BBa_J45017/1
member: https://synbiohub.org/public/igem/BBa_J45004/1

//function/biosynthesis/pyocyanin
type: Collection
persistent identity: function biosynthesis pyocyanin
display id: function_biosynthesis_pyocyanin
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
owned by: james
owned by: myers
member: https://synbiohub.org/public/igem/BBa_I723024/1
member: https://synbiohub.org/public/igem/BBa_I723025/1

//function/biosynthesis/isoprenoid
type: Collection
persistent identity: function biosynthesis isoprenoid
display id: function_biosynthesis_isoprenoid
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
owned by: james
owned by: myers
member: https://synbiohub.org/public/igem/BBa_K152005/1
member: https://synbiohub.org/public/igem/BBa_I742155/1
member: https://synbiohub.org/public/igem/BBa_K115050/1
member: https://synbiohub.org/public/igem/BBa_K118003/1
member: https://synbiohub.org/public/igem/BBa_K849001/1
member: https://synbiohub.org/public/igem/BBa_K118005/1
member: https://synbiohub.org/public/igem/BBa_K118002/1
member: https://synbiohub.org/public/igem/BBa_K152001/1
member: https://synbiohub.org/public/igem/BBa_K849000/1
member: https://synbiohub.org/public/igem/BBa_K115057/1
member: https://synbiohub.org/public/igem/BBa_K849004/1
member: https://synbiohub.org/public/igem/BBa_K118006/1
member: https://synbiohub.org/public/igem/BBa_K849003/1
member: https://synbiohub.org/public/igem/BBa_I742161/1
member: https://synbiohub.org/public/igem/BBa_K118014/1
member: https://synbiohub.org/public/igem/BBa_K152003/1
member: https://synbiohub.org/public/igem/BBa_K152002/1
member: https://synbiohub.org/public/igem/BBa_I742158/1
member: https://synbiohub.org/public/igem/BBa_I742152/1
member: https://synbiohub.org/public/igem/BBa_K115056/1
member: https://synbiohub.org/public/igem/BBa_K118013/1
member: https://synbiohub.org/public/igem/BBa_K343001/1
member: https://synbiohub.org/public/igem/BBa_K118000/1
member: https://synbiohub.org/public/igem/BBa_K343006/1
member: https://synbiohub.org/public/igem/BBa_K118004/1
member: https://synbiohub.org/public/igem/BBa_K118008/1
member: https://synbiohub.org/public/igem/BBa_K118025/1

//function/biosynthesis/cellulose
type: Collection
persistent identity: function biosynthesis cellulose
display id: function_biosynthesis_cellulose
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
owned by: james
owned by: myers
member: https://synbiohub.org/public/igem/BBa_K861110/1
member: https://synbiohub.org/public/igem/BBa_K336020/1
member: https://synbiohub.org/public/igem/BBa_K1044005/1
member: https://synbiohub.org/public/igem/BBa_K861120/1
member: https://synbiohub.org/public/igem/BBa_K1044003/1
member: https://synbiohub.org/public/igem/BBa_K1043005/1
member: https://synbiohub.org/public/igem/BBa_K336052/1
member: https://synbiohub.org/public/igem/BBa_K861100/1
member: https://synbiohub.org/public/igem/BBa_K336051/1
member: https://synbiohub.org/public/igem/BBa_K861130/1
member: https://synbiohub.org/public/igem/BBa_K1044004/1
member: https://synbiohub.org/public/igem/BBa_K1043006/1
member: https://synbiohub.org/public/igem/BBa_K336021/1

//function/biosynthesis/ahl
type: Collection
persistent identity: function biosynthesis ahl
display id: function_biosynthesis_ahl
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
owned by: james
owned by: myers
member: https://synbiohub.org/public/igem/BBa_K1520019/1
member: https://synbiohub.org/public/igem/BBa_C0076/1
member: https://synbiohub.org/public/igem/BBa_C0170/1
member: https://synbiohub.org/public/igem/BBa_I0460/1
member: https://synbiohub.org/public/igem/BBa_C0261/1
member: https://synbiohub.org/public/igem/BBa_K546000/1
member: https://synbiohub.org/public/igem/BBa_I722012/1
member: https://synbiohub.org/public/igem/BBa_K1520022/1
member: https://synbiohub.org/public/igem/BBa_C0070/1
member: https://synbiohub.org/public/igem/BBa_K091109/1
member: https://synbiohub.org/public/igem/BBa_I722011/1
member: https://synbiohub.org/public/igem/BBa_K082008/1
member: https://synbiohub.org/public/igem/BBa_K1520511/1
member: https://synbiohub.org/public/igem/BBa_C0161/1
member: https://synbiohub.org/public/igem/BBa_C0178/1
member: https://synbiohub.org/public/igem/BBa_K082023/1
member: https://synbiohub.org/public/igem/BBa_C0060/1
member: https://synbiohub.org/public/igem/BBa_K082010/1
member: https://synbiohub.org/public/igem/BBa_K1520512/1
member: https://synbiohub.org/public/igem/BBa_C0061/1
member: https://synbiohub.org/public/igem/BBa_K1342001/1
member: https://synbiohub.org/public/igem/BBa_K1520009/1
member: https://synbiohub.org/public/igem/BBa_K1520011/1
member: https://synbiohub.org/public/igem/BBa_K1520504/1
member: https://synbiohub.org/public/igem/BBa_K082007/1
member: https://synbiohub.org/public/igem/BBa_C0078/1
member: https://synbiohub.org/public/igem/BBa_K082035/1
member: https://synbiohub.org/public/igem/BBa_K1520508/1
member: https://synbiohub.org/public/igem/BBa_K082031/1
member: https://synbiohub.org/public/igem/BBa_K1520510/1
member: https://synbiohub.org/public/igem/BBa_I9026/1
member: https://synbiohub.org/public/igem/BBa_K082032/1
member: https://synbiohub.org/public/igem/BBa_K546005/1

//function/biosynthesis
type: Collection
persistent identity: function biosynthesis
display id: function_biosynthesis
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
owned by: james
owned by: myers
member: BBa_K274210
member: ACCsynth
member: sam8
member: rbs+sam5
member: BBa_K118017
member: BBa_K515100
member: BBa_K081009
member: BBa_K1067006
member: BBa_K118015
member: BBa_K727001
member: BBa_K108018
member: BBa_K284999
member: BBa_I716337
member: BBa_I716341
member: BBa_K147003
member: BBa_J45396
member: BBa_K424017
member: BBa_I737020
member: BBa_K1152003
member: BBa_I716336
member: BBa_K1067003
member: //function/biosynthesis/phycocyanobilin
member: //function/biosynthesis/plastic
member: folKE
member: //function/biosynthesis/odorant
member: BBa_K1554002
member: BBa_K1919500
member: BBa_K137017
member: BBa_K274200
member: BBa_K081017
member: BBa_K108028
member: BBa_I716340
member: BisdB
member: BBa_K1067004
member: BBa_K274110
member: ACCoxidase
member: rhlAB
member: BBa_K1554001
member: ninabGen
member: BBa_K118016
member: pabA
member: BBa_K727571
member: BBa_K108029
member: cry11Aa
member: BBa_K1315004
member: //function/biosynthesis/ahl
member: BBa_K1067005
member: BBa_K1686046
member: BBa_I716338
member: sam5
member: BBa_K137006
member: rbs+COMT
member: BBa_K417007
member: //function/biosynthesis/heme
member: aspA
member: rbs+sam8
member: rbs+LIMS1
member: PyrF
member: BBa_K1554003
member: BBa_K156017
member: SAMsynth
member: BBa_K118018
member: CqsA
member: BBa_K130000
member: BBa_K1342002
member: //function/biosynthesis/cellulose
member: BBa_K1497100
member: BisdA
member: BBa_K081008
member: BBa_K108027
member: BBa_K1685011
member: Biosurfact

//function/biosynthesis/butanol
type: Collection
persistent identity: function biosynthesis butanol
display id: function_biosynthesis_butanol
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
owned by: james
owned by: myers
member: https://synbiohub.org/public/igem/BBa_I725011/1
member: https://synbiohub.org/public/igem/BBa_I725014/1
member: https://synbiohub.org/public/igem/BBa_I725015/1
member: https://synbiohub.org/public/igem/BBa_I72512/1
member: https://synbiohub.org/public/igem/BBa_K257000/1
member: https://synbiohub.org/public/igem/BBa_I725013/1
member: https://synbiohub.org/public/igem/BBa_K1462270/1

folB
type: ComponentDefinition
persistent identity: BBa K137009
display id: BBa_K137009
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: folB (dihydroneopterin aldolase)
creator: Victoria Hsiao
created: 2008-06-19T11:00:00Z
modified: 2015-05-08T01:10:08Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _187_
m user id: 0
owner id: 2987
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: folB is part of the L.lactis folate gene cluster.
mutable notes: We were unsure of whether or not the L.lactis RBS would work with E. coli and so we are going to try cloning the entire gene cluster with the RBSs as well as cloning out each gene separately and adding E.coli RBSs.
mutable provenance: L.lactis
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K137009_sequence/1

BBa_K1554002
type: ComponentDefinition
persistent identity: BBa K1554002
display id: BBa_K1554002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: HarFAR
creator: Valencia_UPV team members
created: 2014-09-25T11:00:00Z
modified: 2015-05-28T11:28:27Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1939_
m user id: 4206
owner id: 22386
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: Fatty alcohol reductase
mutable notes: BioBrick compatible
mutable provenance: It comes from Helicoverpa armigera.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1554002_sequence/1

SAMsynth
type: ComponentDefinition
persistent identity: BBa K417000
display id: BBa_K417000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: S-adenosyl methionine (SAM) synthetase
creator: Ben Porebski, Andrew Perry
created: 2010-10-13T11:00:00Z
modified: 2015-05-08T01:12:27Z
discontinued: false
dominant: false
experience: experience/None
group u list: _537_
m user id: 0
owner id: 6167
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This is the protein coding sequence of S-adenosyl methionine synthetase, which catalyses the formation of S-adenosyl methionine synthetase (AdoMet) from ATP and methionine (ATP + L-methionine + H(2)O <=> phosphate + diphosphate + S-adenosyl-L-methionine). Other names for this enzyme are AdoMet synthetase, methionine adenosyltransferase. It is the product of the metK gene in E. coli.
mutable notes: The genomic sequence corresponding to this part does not contain any illegal restriction sites with respect to RFC10, so no mutagenesis of the native sequence was required.
mutable provenance: Part was cloned from genomic DNA of Escherichia coli strain BL21(DE3) using PCR.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: stop
sequence annotation: SAMsynth
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_K417000_sequence/1

stop
type: SequenceAnnotation
persistent identity: annotation2085975
display id: annotation2085975
version: 1
top level: SAMsynth
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K417000/annotation2085975/range2085975/1

https://synbiohub.org/public/igem/BBa_K417000/annotation2085975/range2085975/1
type: Range
persistent identity: range2085975
display id: range2085975
version: 1
direction: direction/forward
top level: SAMsynth
start: 1153
end: 1153
orientation: inline

SAMsynth
type: SequenceAnnotation
persistent identity: annotation2085973
display id: annotation2085973
version: 1
top level: SAMsynth
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K417000/annotation2085973/range2085973/1

https://synbiohub.org/public/igem/BBa_K417000/annotation2085973/range2085973/1
type: Range
persistent identity: range2085973
display id: range2085973
version: 1
direction: direction/forward
top level: SAMsynth
start: 1
end: 1155
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2085974
display id: annotation2085974
version: 1
top level: SAMsynth
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K417000/annotation2085974/range2085974/1

https://synbiohub.org/public/igem/BBa_K417000/annotation2085974/range2085974/1
type: Range
persistent identity: range2085974
display id: range2085974
version: 1
direction: direction/forward
top level: SAMsynth
start: 1
end: 1
orientation: inline

BBa_K274110
type: ComponentDefinition
persistent identity: BBa K274110
display id: BBa_K274110
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CrtEBI under constitutive promoter
creator: Siming Ma
created: 2009-09-17T11:00:00Z
modified: 2015-05-08T01:11:45Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _374_
m user id: 0
owner id: 5416
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This Biobrick is created by putting enzyme cassette CrtEBI (with individual rbs) of Part BBa_K274100 under constitutive promoter R0011. Enzyme cassette CrtEBI (with individual rbs) of Part BBa_K274110 converts colourless farnesyl pyrophosphate to red lycopene (via intermediates geranylgeranyl pyroiphosphate and phytoene). Amount of lycopene produced can be measured by photospectrometer with absorbance at 475nm (lycopene extraction using acetone).
mutable notes: The colourless precursor, farnesyl pyrophosphate, is naturally produced in E.coli using Non-mevalonate Pathway (from pyruvate and glyceraldehyde-3-phosphate). Our experiences showed that this Biobrick works better in E.coli strains with up-regulated level of precursors. E.coli strain MG1655 demonostrates higher level of pigment (lycopene) production than strain TOP10.
mutable provenance: Gene coding sequences come from Pantoea ananatis (formerly Erwinia uredovora) (Accession number D90087), a type of Gram negative Enterobacteriaceae. Please refer to Part BBa_K274100 for more information on individual components.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_R0011
component: BBa_J15001
component: BBa_K118002
component: BBa_I742151
component: BBa_K118003
component: BBa_K118012
sequence annotation: BBa_I742151
sequence annotation: BBa_K118012
sequence annotation: BBa_K118003
sequence annotation: BBa_K118002
sequence annotation: BBa_J15001
sequence annotation: BBa_R0011
sequence: https://synbiohub.org/public/igem/BBa_K274110_sequence/1

BBa_R0011
type: Component
persistent identity: component2021362
display id: component2021362
version: 1
top level: BBa_K274110
access: public
definition: https://synbiohub.org/public/igem/BBa_R0011/1

BBa_J15001
type: Component
persistent identity: component2021371
display id: component2021371
version: 1
top level: BBa_K274110
access: public
definition: https://synbiohub.org/public/igem/BBa_J15001/1

BBa_K118002
type: Component
persistent identity: component2021373
display id: component2021373
version: 1
top level: BBa_K274110
access: public
definition: https://synbiohub.org/public/igem/BBa_K118002/1

BBa_J15001
type: Component
persistent identity: component2021376
display id: component2021376
version: 1
top level: BBa_K274110
access: public
definition: https://synbiohub.org/public/igem/BBa_J15001/1

BBa_I742151
type: Component
persistent identity: component2021368
display id: component2021368
version: 1
top level: BBa_K274110
access: public
definition: https://synbiohub.org/public/igem/BBa_I742151/1

BBa_K118003
type: Component
persistent identity: component2021380
display id: component2021380
version: 1
top level: BBa_K274110
access: public
definition: https://synbiohub.org/public/igem/BBa_K118003/1

BBa_K118012
type: Component
persistent identity: component2021367
display id: component2021367
version: 1
top level: BBa_K274110
access: public
definition: https://synbiohub.org/public/igem/BBa_K118012/1

BBa_I742151
type: SequenceAnnotation
persistent identity: annotation2021368
display id: annotation2021368
version: 1
top level: BBa_K274110
location: https://synbiohub.org/public/igem/BBa_K274110/annotation2021368/range2021368/1
component: BBa_I742151

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type: Range
persistent identity: range2021368
display id: range2021368
version: 1
direction: direction/reverse
top level: BBa_K274110
start: 74
end: 985
orientation: inline

BBa_K118012
type: SequenceAnnotation
persistent identity: annotation2021367
display id: annotation2021367
version: 1
top level: BBa_K274110
location: https://synbiohub.org/public/igem/BBa_K274110/annotation2021367/range2021367/1
component: BBa_K118012

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type: Range
persistent identity: range2021367
display id: range2021367
version: 1
direction: direction/reverse
top level: BBa_K274110
start: 64
end: 67
orientation: inline

BBa_K118003
type: SequenceAnnotation
persistent identity: annotation2021380
display id: annotation2021380
version: 1
top level: BBa_K274110
location: https://synbiohub.org/public/igem/BBa_K274110/annotation2021380/range2021380/1
component: BBa_K118003

https://synbiohub.org/public/igem/BBa_K274110/annotation2021380/range2021380/1
type: Range
persistent identity: range2021380
display id: range2021380
version: 1
direction: direction/reverse
top level: BBa_K274110
start: 1967
end: 3448
orientation: inline

BBa_K118002
type: SequenceAnnotation
persistent identity: annotation2021373
display id: annotation2021373
version: 1
top level: BBa_K274110
location: https://synbiohub.org/public/igem/BBa_K274110/annotation2021373/range2021373/1
component: BBa_K118002

https://synbiohub.org/public/igem/BBa_K274110/annotation2021373/range2021373/1
type: Range
persistent identity: range2021373
display id: range2021373
version: 1
direction: direction/reverse
top level: BBa_K274110
start: 1010
end: 1942
orientation: inline

BBa_J15001
type: SequenceAnnotation
persistent identity: annotation2021376
display id: annotation2021376
version: 1
top level: BBa_K274110
location: https://synbiohub.org/public/igem/BBa_K274110/annotation2021376/range2021376/1
component: BBa_J15001

https://synbiohub.org/public/igem/BBa_K274110/annotation2021376/range2021376/1
type: Range
persistent identity: range2021376
display id: range2021376
version: 1
direction: direction/reverse
top level: BBa_K274110
start: 1951
end: 1960
orientation: inline

BBa_J15001
type: SequenceAnnotation
persistent identity: annotation2021371
display id: annotation2021371
version: 1
top level: BBa_K274110
location: https://synbiohub.org/public/igem/BBa_K274110/annotation2021371/range2021371/1
component: BBa_J15001

https://synbiohub.org/public/igem/BBa_K274110/annotation2021371/range2021371/1
type: Range
persistent identity: range2021371
display id: range2021371
version: 1
direction: direction/reverse
top level: BBa_K274110
start: 994
end: 1003
orientation: inline

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation2021362
display id: annotation2021362
version: 1
top level: BBa_K274110
location: https://synbiohub.org/public/igem/BBa_K274110/annotation2021362/range2021362/1
component: BBa_R0011

https://synbiohub.org/public/igem/BBa_K274110/annotation2021362/range2021362/1
type: Range
persistent identity: range2021362
display id: range2021362
version: 1
direction: direction/forward
top level: BBa_K274110
start: 1
end: 54
orientation: inline

BBa_K1315004
type: ComponentDefinition
persistent identity: BBa K1315004
display id: BBa_K1315004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: ydeH (dgcZ)
creator: Dimitrios Michailidis
created: 2014-09-24T11:00:00Z
modified: 2015-05-08T01:09:49Z
discontinued: true
dominant: false
experience: experience/None
group u list: _1690_
m user id: 0
owner id: 21812
owning group id: 9
sample status: Discontinued
status: status/Deleted
bookmark: false
mutable description: Diguanylate cyclases produce cyclic diGMP (c-di-GMP), a secondary messenger. Diguanylate Cyclase (DgcZ), also called YdeH, is present in Escherichia coli and the stimulus that controls it is Zinc.
mutable notes: The sequence did not have any restriction sites to remove so it was immediately PCRed out of the genome and had the registry prefix and suffix added to it. It was then ligated into the pSB1C3 vector. YdeH however, is toxic to E. coli cells.
mutable provenance: The source of this part is Escherichia coli MC40100 genomic DNA which was provided by the Tracy Palmer lab in the Molecular Microbiology department of the University of Dundee, UK.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: YdeH
sequence: https://synbiohub.org/public/igem/BBa_K1315004_sequence/1

YdeH
type: SequenceAnnotation
persistent identity: annotation2386526
display id: annotation2386526
version: 1
top level: BBa_K1315004
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1315004/annotation2386526/range2386526/1

https://synbiohub.org/public/igem/BBa_K1315004/annotation2386526/range2386526/1
type: Range
persistent identity: range2386526
display id: range2386526
version: 1
direction: direction/forward
top level: BBa_K1315004
start: 1
end: 894
orientation: inline

ACCsynth
type: ComponentDefinition
persistent identity: BBa K417001
display id: BBa_K417001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: 1-aminocyclopropane-1-carboxylate (ACC) synthase
creator: Ben Porebski, Andrew Perry
created: 2010-10-19T11:00:00Z
modified: 2015-05-08T01:12:28Z
discontinued: false
dominant: false
experience: experience/None
group u list: _537_
m user id: 0
owner id: 6513
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is the protein coding sequence of 1-aminocyclopropane-1-carboxylate synthase, which catalyses the formation of 1-aminocyclopropane-1-carboxylate and methylthioadenosine from S-adenosyl methionine (AdoMet). (S-adenosyl-L-methionine <=> 1-aminocyclopropane-1-carboxylate + methylthioadenosine). This enzyme is commonly referred to as ACC synthase.
mutable notes: Removal of the C-terminal amino acids. According to White, M. F., Vasquez, J., Yang, S. F., & Kirsch, J. F. (1994). PNAS, 91(26), 12428-32. (http://www.ncbi.nlm.nih.gov/pubmed/7809054) - this truncation has been reported to have no effect on specific activity.
mutable provenance: Malus domestica (Apple), codon optimized for E. coli, with the C-terminal amino acids -YYNVPEVNGGSQSSHLSHSRRQSLTKWVSRLSFDDRGPIPGR* removed
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Stop
sequence annotation: ACC Synth
sequence annotation: Start
sequence: https://synbiohub.org/public/igem/BBa_K417001_sequence/1

Stop
type: SequenceAnnotation
persistent identity: annotation2090377
display id: annotation2090377
version: 1
top level: ACCsynth
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K417001/annotation2090377/range2090377/1

https://synbiohub.org/public/igem/BBa_K417001/annotation2090377/range2090377/1
type: Range
persistent identity: range2090377
display id: range2090377
version: 1
direction: direction/forward
top level: ACCsynth
start: 1288
end: 1290
orientation: inline

ACC Synth
type: SequenceAnnotation
persistent identity: annotation2090376
display id: annotation2090376
version: 1
top level: ACCsynth
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K417001/annotation2090376/range2090376/1

https://synbiohub.org/public/igem/BBa_K417001/annotation2090376/range2090376/1
type: Range
persistent identity: range2090376
display id: range2090376
version: 1
direction: direction/forward
top level: ACCsynth
start: 1
end: 1290
orientation: inline

Start
type: SequenceAnnotation
persistent identity: annotation2090375
display id: annotation2090375
version: 1
top level: ACCsynth
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K417001/annotation2090375/range2090375/1

https://synbiohub.org/public/igem/BBa_K417001/annotation2090375/range2090375/1
type: Range
persistent identity: range2090375
display id: range2090375
version: 1
direction: direction/forward
top level: ACCsynth
start: 1
end: 3
orientation: inline

ninabGen
type: ComponentDefinition
persistent identity: BBa K343005
display id: BBa_K343005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Beta-carotene 15
creator: Christian Kurtzhals
created: 2010-09-06T11:00:00Z
modified: 2015-05-08T01:12:08Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _465_
m user id: 0
owner id: 6083
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: The Beta-carotene 15'15'-monooxygenase generator from the D. melanogaster ninaB gene, cleaves beta-carotene into two retinal molecules. Used together with BBa_274210 (beta-carotene generator) it should induce retinal biosynthesis in e. coli.
mutable notes: A constitutive sigma-70 promotor was chosen. Terminator is lacking on this part.
mutable provenance: [link:https://dgrc.cgb.indiana.edu/product/View?product=4630 Drosophila genomics ressource centre], Indiana University. Found via [link:http://www.flybase.org flybase], ID: [link:http://flybase.org/reports/FBcl0129473.html FBcl0129473]
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0034
component: BBa_R0040
component: BBa_K343001
sequence annotation: BBa_R0040
sequence annotation: BBa_B0034
sequence annotation: BBa_K343001
sequence: https://synbiohub.org/public/igem/BBa_K343005_sequence/1

BBa_B0034
type: Component
persistent identity: component2079706
display id: component2079706
version: 1
top level: ninabGen
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_R0040
type: Component
persistent identity: component2079700
display id: component2079700
version: 1
top level: ninabGen
access: public
definition: https://synbiohub.org/public/igem/BBa_R0040/1

BBa_K343001
type: Component
persistent identity: component2079710
display id: component2079710
version: 1
top level: ninabGen
access: public
definition: https://synbiohub.org/public/igem/BBa_K343001/1

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2079700
display id: annotation2079700
version: 1
top level: ninabGen
location: https://synbiohub.org/public/igem/BBa_K343005/annotation2079700/range2079700/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_K343005/annotation2079700/range2079700/1
type: Range
persistent identity: range2079700
display id: range2079700
version: 1
direction: direction/forward
top level: ninabGen
start: 1
end: 54
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2079706
display id: annotation2079706
version: 1
top level: ninabGen
location: https://synbiohub.org/public/igem/BBa_K343005/annotation2079706/range2079706/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K343005/annotation2079706/range2079706/1
type: Range
persistent identity: range2079706
display id: range2079706
version: 1
direction: direction/reverse
top level: ninabGen
start: 63
end: 74
orientation: inline

BBa_K343001
type: SequenceAnnotation
persistent identity: annotation2079710
display id: annotation2079710
version: 1
top level: ninabGen
location: https://synbiohub.org/public/igem/BBa_K343005/annotation2079710/range2079710/1
component: BBa_K343001

https://synbiohub.org/public/igem/BBa_K343005/annotation2079710/range2079710/1
type: Range
persistent identity: range2079710
display id: range2079710
version: 1
direction: direction/reverse
top level: ninabGen
start: 81
end: 1943
orientation: inline

BBa_K137006
type: ComponentDefinition
persistent identity: BBa K137006
display id: BBa_K137006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pabB (from pABA synthesis)
creator: Victoria Hsiao
created: 2008-06-19T11:00:00Z
modified: 2015-05-08T01:10:08Z
discontinued: false
dominant: false
experience: experience/None
group u list: _187_
m user id: 0
owner id: 2987
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: PabB from L.lactis is a fusion gene of both the usual pabB and pabC in the pABA synthesis pathway from chorismate.
mutable notes: The start codon of pabB in L.lactis is TTG, which cannot be used in E.coli. So we designed the primer such that the first T was omitted and the A from the xba1 primer make an ATG start codon.
mutable provenance: L.lactis genome
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Changed T to A
sequence: https://synbiohub.org/public/igem/BBa_K137006_sequence/1

Changed T to A
type: SequenceAnnotation
persistent identity: annotation1971718
display id: annotation1971718
version: 1
top level: BBa_K137006
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K137006/annotation1971718/range1971718/1

https://synbiohub.org/public/igem/BBa_K137006/annotation1971718/range1971718/1
type: Range
persistent identity: range1971718
display id: range1971718
version: 1
direction: direction/forward
top level: BBa_K137006
start: 1
end: 1
orientation: inline

BBa_I716341
type: ComponentDefinition
persistent identity: BBa I716341
display id: BBa_I716341
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: rbs-ThpB-rbs-ThpC-rbs-ThpD-TT
creator: nhu nguyen
created: 2007-08-13T11:00:00Z
modified: 2015-08-31T04:07:52Z
discontinued: false
dominant: false
experience: experience/None
group u list: _110_
m user id: 0
owner id: 2095
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: rbs-ThpB-rbs-ThpC-rbs-ThpD-TT will help produce hydroxyectoine to withstand freeze dry.
mutable notes: Digest I716340 (BglII/XhoI, 2057bp, 1058bp, S) Sub into I716339 (BamHI/XhoI, 3759bp, 9bp, L) Product is I716341
mutable provenance: {{BerkiGEM2007-Biobrick2.0Intro}}
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence: https://synbiohub.org/public/igem/BBa_I716341_sequence/1

BBa_K274210
type: ComponentDefinition
persistent identity: BBa K274210
display id: BBa_K274210
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CrtEBIY under constitutive promoter
creator: Siming Ma
created: 2009-09-18T11:00:00Z
modified: 2015-05-08T01:11:45Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _374_
m user id: 0
owner id: 5416
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This Biobrick is created by putting enzyme cassette CrtEBIY (with individual rbs) of Part BBa_K274200 under constitutive promoter R0011. Enzyme cassette CrtEBIY (with individual rbs) of Part BBa_K274200 converts colourless farnesyl pyrophosphate to orange beta-carotene (via intermediates geranylgeranyl pyroiphosphate, phytoene and lycopene). Amount of beta-carotene produced can be measured by photospectrometer with absorbance at 455nm (beta-carotene extraction using acetone). Sequence and Features
mutable notes: The colourless precursor, farnesyl pyrophosphate, is naturally produced in E.coli using Non-mevalonate Pathway (from pyruvate and glyceraldehyde-3-phosphate). Our experiences showed that this Biobrick works better in E.coli strains with up-regulated level of precursors. E.coli strain MG1655 demonostrates higher level of pigment (lycopene) production than strain TOP10.
mutable provenance: Gene coding sequences come from Pantoea ananatis (formerly Erwinia uredovora) (Accession number D90087), a type of Gram negative Enterobacteriaceae. Please refer to Part BBa_K274200 for more information on individual components.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K118012
component: BBa_I742151
component: BBa_J15001
component: BBa_R0011
component: BBa_K118008
component: BBa_K118002
component: BBa_K118003
sequence annotation: BBa_K118003
sequence annotation: BBa_J15001
sequence annotation: BBa_K118008
sequence annotation: BBa_K118012
sequence annotation: BBa_R0011
sequence annotation: BBa_I742151
sequence annotation: BBa_K118002
sequence: https://synbiohub.org/public/igem/BBa_K274210_sequence/1

BBa_K118012
type: Component
persistent identity: component2021563
display id: component2021563
version: 1
top level: BBa_K274210
access: public
definition: https://synbiohub.org/public/igem/BBa_K118012/1

BBa_I742151
type: Component
persistent identity: component2021564
display id: component2021564
version: 1
top level: BBa_K274210
access: public
definition: https://synbiohub.org/public/igem/BBa_I742151/1

BBa_J15001
type: Component
persistent identity: component2021572
display id: component2021572
version: 1
top level: BBa_K274210
access: public
definition: https://synbiohub.org/public/igem/BBa_J15001/1

BBa_J15001
type: Component
persistent identity: component2021567
display id: component2021567
version: 1
top level: BBa_K274210
access: public
definition: https://synbiohub.org/public/igem/BBa_J15001/1

BBa_K118012
type: Component
persistent identity: component2021577
display id: component2021577
version: 1
top level: BBa_K274210
access: public
definition: https://synbiohub.org/public/igem/BBa_K118012/1

BBa_R0011
type: Component
persistent identity: component2021558
display id: component2021558
version: 1
top level: BBa_K274210
access: public
definition: https://synbiohub.org/public/igem/BBa_R0011/1

BBa_K118008
type: Component
persistent identity: component2021579
display id: component2021579
version: 1
top level: BBa_K274210
access: public
definition: https://synbiohub.org/public/igem/BBa_K118008/1

BBa_K118002
type: Component
persistent identity: component2021569
display id: component2021569
version: 1
top level: BBa_K274210
access: public
definition: https://synbiohub.org/public/igem/BBa_K118002/1

BBa_K118003
type: Component
persistent identity: component2021576
display id: component2021576
version: 1
top level: BBa_K274210
access: public
definition: https://synbiohub.org/public/igem/BBa_K118003/1

BBa_K118003
type: SequenceAnnotation
persistent identity: annotation2021576
display id: annotation2021576
version: 1
top level: BBa_K274210
location: https://synbiohub.org/public/igem/BBa_K274210/annotation2021576/range2021576/1
component: BBa_K118003

https://synbiohub.org/public/igem/BBa_K274210/annotation2021576/range2021576/1
type: Range
persistent identity: range2021576
display id: range2021576
version: 1
direction: direction/reverse
top level: BBa_K274210
start: 1967
end: 3448
orientation: inline

BBa_J15001
type: SequenceAnnotation
persistent identity: annotation2021572
display id: annotation2021572
version: 1
top level: BBa_K274210
location: https://synbiohub.org/public/igem/BBa_K274210/annotation2021572/range2021572/1
component: BBa_J15001

https://synbiohub.org/public/igem/BBa_K274210/annotation2021572/range2021572/1
type: Range
persistent identity: range2021572
display id: range2021572
version: 1
direction: direction/reverse
top level: BBa_K274210
start: 1951
end: 1960
orientation: inline

BBa_K118008
type: SequenceAnnotation
persistent identity: annotation2021579
display id: annotation2021579
version: 1
top level: BBa_K274210
location: https://synbiohub.org/public/igem/BBa_K274210/annotation2021579/range2021579/1
component: BBa_K118008

https://synbiohub.org/public/igem/BBa_K274210/annotation2021579/range2021579/1
type: Range
persistent identity: range2021579
display id: range2021579
version: 1
direction: direction/reverse
top level: BBa_K274210
start: 3467
end: 4618
orientation: inline

BBa_K118012
type: SequenceAnnotation
persistent identity: annotation2021563
display id: annotation2021563
version: 1
top level: BBa_K274210
location: https://synbiohub.org/public/igem/BBa_K274210/annotation2021563/range2021563/1
component: BBa_K118012

https://synbiohub.org/public/igem/BBa_K274210/annotation2021563/range2021563/1
type: Range
persistent identity: range2021563
display id: range2021563
version: 1
direction: direction/reverse
top level: BBa_K274210
start: 64
end: 67
orientation: inline

BBa_K118012
type: SequenceAnnotation
persistent identity: annotation2021577
display id: annotation2021577
version: 1
top level: BBa_K274210
location: https://synbiohub.org/public/igem/BBa_K274210/annotation2021577/range2021577/1
component: BBa_K118012

https://synbiohub.org/public/igem/BBa_K274210/annotation2021577/range2021577/1
type: Range
persistent identity: range2021577
display id: range2021577
version: 1
direction: direction/reverse
top level: BBa_K274210
start: 3457
end: 3460
orientation: inline

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation2021558
display id: annotation2021558
version: 1
top level: BBa_K274210
location: https://synbiohub.org/public/igem/BBa_K274210/annotation2021558/range2021558/1
component: BBa_R0011

https://synbiohub.org/public/igem/BBa_K274210/annotation2021558/range2021558/1
type: Range
persistent identity: range2021558
display id: range2021558
version: 1
direction: direction/forward
top level: BBa_K274210
start: 1
end: 54
orientation: inline

BBa_J15001
type: SequenceAnnotation
persistent identity: annotation2021567
display id: annotation2021567
version: 1
top level: BBa_K274210
location: https://synbiohub.org/public/igem/BBa_K274210/annotation2021567/range2021567/1
component: BBa_J15001

https://synbiohub.org/public/igem/BBa_K274210/annotation2021567/range2021567/1
type: Range
persistent identity: range2021567
display id: range2021567
version: 1
direction: direction/reverse
top level: BBa_K274210
start: 994
end: 1003
orientation: inline

BBa_I742151
type: SequenceAnnotation
persistent identity: annotation2021564
display id: annotation2021564
version: 1
top level: BBa_K274210
location: https://synbiohub.org/public/igem/BBa_K274210/annotation2021564/range2021564/1
component: BBa_I742151

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type: Range
persistent identity: range2021564
display id: range2021564
version: 1
direction: direction/reverse
top level: BBa_K274210
start: 74
end: 985
orientation: inline

BBa_K118002
type: SequenceAnnotation
persistent identity: annotation2021569
display id: annotation2021569
version: 1
top level: BBa_K274210
location: https://synbiohub.org/public/igem/BBa_K274210/annotation2021569/range2021569/1
component: BBa_K118002

https://synbiohub.org/public/igem/BBa_K274210/annotation2021569/range2021569/1
type: Range
persistent identity: range2021569
display id: range2021569
version: 1
direction: direction/reverse
top level: BBa_K274210
start: 1010
end: 1942
orientation: inline

BBa_K424017
type: ComponentDefinition
persistent identity: BBa K424017
display id: BBa_K424017
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Test plataform for rhamnosyltransferase BioBrick (Rh1AB_BB) expression in E. coli
creator: Ernesto G??mez
created: 2010-10-10T11:00:00Z
modified: 2015-05-08T01:12:28Z
discontinued: false
dominant: false
experience: experience/None
group u list: _545_
m user id: 0
owner id: 7119
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: <here is a test of the composite system
mutable notes: none
mutable provenance: nope
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0034
component: BBa_E0040
component: BBa_B0010
component: BBa_K424018
component: BBa_B0012
component: BBa_I719005
sequence annotation: BBa_B0010
sequence annotation: BBa_K424018
sequence annotation: BBa_I719005
sequence annotation: BBa_E0040
sequence annotation: BBa_B0012
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K424017_sequence/1

BBa_B0034
type: Component
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display id: component2086183
version: 1
top level: BBa_K424017
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_E0040
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definition: https://synbiohub.org/public/igem/BBa_E0040/1

BBa_B0010
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display id: component2086196
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access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_K424018
type: Component
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display id: component2086193
version: 1
top level: BBa_K424017
access: public
definition: rhlAB

BBa_B0012
type: Component
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version: 1
top level: BBa_K424017
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_I719005
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display id: component2086181
version: 1
top level: BBa_K424017
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definition: https://synbiohub.org/public/igem/BBa_I719005/1

BBa_B0010
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BBa_E0040
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version: 1
top level: BBa_K424017
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component: BBa_E0040

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BBa_B0012
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top level: BBa_K424017
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component: BBa_B0012

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BBa_B0034
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version: 1
top level: BBa_K424017
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component: BBa_B0034

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BBa_K1067003
type: ComponentDefinition
persistent identity: BBa K1067003
display id: BBa_K1067003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ammonium monooxygenase from Nitrosomonas europaea
creator: Kristian Davidsen and Helen Cook
created: 2013-09-18T11:00:00Z
modified: 2015-05-08T01:09:00Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1376_
m user id: 0
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sample status: In stock
status: status/Available
bookmark: false
mutable description: hjjkl
mutable notes: uiop
mutable provenance: jhkjl
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: stop
sequence annotation: start
sequence annotation: amoC
sequence annotation: amoB
sequence annotation: amoA
sequence: https://synbiohub.org/public/igem/BBa_K1067003_sequence/1

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type: SequenceAnnotation
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amoC
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start: 1
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amoB
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version: 1
top level: BBa_K1067003
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role: feature/protein
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amoA
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start: 814
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stop
type: SequenceAnnotation
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type: Range
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version: 1
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start: 3072
end: 3074
orientation: inline

BBa_K417006
type: ComponentDefinition
persistent identity: BBa K417006
display id: BBa_K417006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LacI ethylene generator
creator: Benjamin Porebski
created: 2010-10-25T11:00:00Z
modified: 2015-05-08T01:12:28Z
discontinued: false
dominant: false
group u list: _537_
m user id: 0
owner id: 6513
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: LacI regulated ethylene generator that uses 3 enzymes, blah
mutable notes: Nil
mutable provenance: E. coli genomic DNA, Apple and Tomato
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_K417003
component: BBa_K417004
component: BBa_B0015
component: BBa_K417005
component: BBa_R0010
sequence annotation: BBa_K417005
sequence annotation: BBa_R0010
sequence annotation: BBa_K417004
sequence annotation: BBa_K417003
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_K417006_sequence/1

BBa_K417003
type: Component
persistent identity: component2101907
display id: component2101907
version: 1
top level: BBa_K417006
access: public
definition: https://synbiohub.org/public/igem/BBa_K417003/1

BBa_K417004
type: Component
persistent identity: component2101914
display id: component2101914
version: 1
top level: BBa_K417006
access: public
definition: https://synbiohub.org/public/igem/BBa_K417004/1

BBa_B0015
type: Component
persistent identity: component2101928
display id: component2101928
version: 1
top level: BBa_K417006
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_K417005
type: Component
persistent identity: component2101921
display id: component2101921
version: 1
top level: BBa_K417006
access: public
definition: https://synbiohub.org/public/igem/BBa_K417005/1

BBa_R0010
type: Component
persistent identity: component2101895
display id: component2101895
version: 1
top level: BBa_K417006
access: public
definition: https://synbiohub.org/public/igem/BBa_R0010/1

BBa_K417005
type: SequenceAnnotation
persistent identity: annotation2101921
display id: annotation2101921
version: 1
top level: BBa_K417006
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component: BBa_K417005

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type: Range
persistent identity: range2101921
display id: range2101921
version: 1
direction: direction/reverse
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start: 2706
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orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation2101895
display id: annotation2101895
version: 1
top level: BBa_K417006
location: https://synbiohub.org/public/igem/BBa_K417006/annotation2101895/range2101895/1
component: BBa_R0010

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type: Range
persistent identity: range2101895
display id: range2101895
version: 1
direction: direction/forward
top level: BBa_K417006
start: 1
end: 200
orientation: inline

BBa_K417004
type: SequenceAnnotation
persistent identity: annotation2101914
display id: annotation2101914
version: 1
top level: BBa_K417006
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version: 1
direction: direction/reverse
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start: 1390
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type: SequenceAnnotation
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version: 1
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start: 209
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BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2101928
display id: annotation2101928
version: 1
top level: BBa_K417006
location: https://synbiohub.org/public/igem/BBa_K417006/annotation2101928/range2101928/1
component: BBa_B0015

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type: Range
persistent identity: range2101928
display id: range2101928
version: 1
direction: direction/reverse
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start: 3680
end: 3808
orientation: inline

BBa_K156017
type: ComponentDefinition
persistent identity: BBa K156017
display id: BBa_K156017
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS + phaC1
creator: George McArthur IV
created: 2008-10-28T12:00:00Z
modified: 2015-05-08T01:10:54Z
discontinued: false
dominant: false
experience: experience/None
group u list: _246_
m user id: 0
owner id: 1475
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This composite part is made of a standard ribosomal binding site and a part that codes for a polyhydroxybutyrate (PHB) synthase, which catalyzes the polymerization of hydroxybutyryl-CoA.
mutable notes: None.
mutable provenance: This is a composite part.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_K156014
component: BBa_B0032
sequence annotation: BBa_K156014
sequence annotation: BBa_B0032
sequence: https://synbiohub.org/public/igem/BBa_K156017_sequence/1

BBa_K156014
type: Component
persistent identity: component1993504
display id: component1993504
version: 1
top level: BBa_K156017
access: public
definition: https://synbiohub.org/public/igem/BBa_K156014/1

BBa_B0032
type: Component
persistent identity: component1993502
display id: component1993502
version: 1
top level: BBa_K156017
access: public
definition: https://synbiohub.org/public/igem/BBa_B0032/1

BBa_K156014
type: SequenceAnnotation
persistent identity: annotation1993504
display id: annotation1993504
version: 1
top level: BBa_K156017
location: https://synbiohub.org/public/igem/BBa_K156017/annotation1993504/range1993504/1
component: BBa_K156014

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type: Range
persistent identity: range1993504
display id: range1993504
version: 1
direction: direction/reverse
top level: BBa_K156017
start: 20
end: 1368
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation1993502
display id: annotation1993502
version: 1
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location: https://synbiohub.org/public/igem/BBa_K156017/annotation1993502/range1993502/1
component: BBa_B0032

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type: Range
persistent identity: range1993502
display id: range1993502
version: 1
direction: direction/forward
top level: BBa_K156017
start: 1
end: 13
orientation: inline

BBa_K108026
type: ComponentDefinition
persistent identity: BBa K108026
display id: BBa_K108026
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: XylA
creator: Qi Liu
created: 2008-10-22T11:00:00Z
modified: 2015-05-08T01:09:04Z
discontinued: false
dominant: false
experience: experience/None
group u list: _222_
m user id: 0
owner id: 2671
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: coming...
mutable notes: PCR using primers with biobrick prefix and surfix, and then cloned into pSB1AC3.
mutable provenance: pWW0
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: XylA
sequence: https://synbiohub.org/public/igem/BBa_K108026_sequence/1

XylA
type: SequenceAnnotation
persistent identity: annotation1986981
display id: annotation1986981
version: 1
top level: BBa_K108026
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K108026/annotation1986981/range1986981/1

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type: Range
persistent identity: range1986981
display id: range1986981
version: 1
direction: direction/forward
top level: BBa_K108026
start: 1
end: 1050
orientation: inline

BBa_I716339
type: ComponentDefinition
persistent identity: BBa I716339
display id: BBa_I716339
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: rbs-thpB-rbs-ThpC
creator: nhu nguyen
created: 2007-08-13T11:00:00Z
modified: 2015-08-31T04:07:52Z
discontinued: false
dominant: false
experience: experience/None
group u list: _110_
m user id: 0
owner id: 2095
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: rbs-ThpB-rbs-ThpC will help in the production of hydroxyectoine to withstand freeze dry.
mutable notes: Digest I716337 (BglII/XhoI, 2057bp, 428bp, S) Sub into I716336 (BamHI/XhoI, 3340bp, 9bp, L) Product is I716339
mutable provenance: {{BerkiGEM2007-Biobrick2.0Intro}}
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
sequence: https://synbiohub.org/public/igem/BBa_I716339_sequence/1

Biosurfact
type: ComponentDefinition
persistent identity: BBa K653000
display id: BBa_K653000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Re-designing "The Biosurfactator" The first Central American BioBrick
creator: Team Panama 2011
created: 2011-09-25T11:00:00Z
modified: 2015-05-08T01:13:00Z
discontinued: false
dominant: false
experience: experience/None
group u list: _545_830_
m user id: 0
owner id: 7119
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: Pseudomonas aeruginosa species of bacteria produce the natural rhamnosyltransferase gene complex (RhlAB). This is the key enzyme responsible for transferring the rhamnose moiety to the b-hydroxyalkanoic acid moiety to biosynthesize rhamnolipid, which is a biomolecule with surfactant properties, but the natural RhlAB gene has illegal restriction sites that make it incompatible with the Assembly Standard Protocol 10, specifically the Pstl1 restriction sites. The design and construction of the rhamnosyltransferase gene into a BioBrick part was the main goal and achievement of the iGEM Panama Team 2010, but since the beginning of this year???s iGEM project we failed in producing the rhamnolipid compound into our E. coli based-factory, because our last year???s BioBrick appeared to have been denaturalized. We then ordered our BioBrick part BBa_K424018 (iGEM Panama team 2010) from The Registry and once it arrived we ran some tests and we noticed that the part was no longer fitted into the plasmid backbone. As scientist and iGEMers, we have the responsibility to deliver a functional BioBrick, thus according to iGEM???s competition new slogan, ???Quality not Quantity???, we have embraced the challenge of re-designing and re-building the same BioBrick part from last year in order to comply this new standard based on quality. How to use it as a BioBrick? : We developed this standarized part to be inserted into E. coli for rhamnolipid production through the mutation of the gene that is responsible to synthesize the key enzyme rhamnosyltranferase (RhlAB) to be compatible with Assembly Standard 10. We have achieved this by performing several silent mutations using the QuikLighting Multi Site-Directed Mutagenesis Kit from Stratagene. This rhamnosyltransferase BioBrick (Rh1AB_BB) is ready to be tested on an expression plasmid device following the Assembly Standard Protocol 10.
mutable notes: The RhlAB gene complex has shown illegal restriction sites according to the assembly standard protocol 10. We faced this problem by applying the mutagenesis protocol that consist in doing silent mutations that change the nucleotides of our rhlAB gene that are cut by the Pstl restriction enzyme. This will give us a standardized coding sequence of the rhlAB gene compatible with the Assembly Standard protocol 10 and a new BioBrick part for the Registry.
mutable provenance: Pseudomonas aeruginosa from INDICASAT lab. and the genomic sequence of RhlAB was reviwed in genBank (ID 878955 and 878954) http://www.ncbi.nlm.nih.gov/nuccore/L28170.1
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Rhamnosyltranferase A
sequence annotation: Silent Mutation
sequence annotation: Rhamnosyltransferase B
sequence annotation: Primer
sequence annotation: Rhamnosyltransferase gene complex 1
sequence annotation: BBa_ K653000 RhlAB Panama
sequence: https://synbiohub.org/public/igem/BBa_K653000_sequence/1

Rhamnosyltranferase A
type: SequenceAnnotation
persistent identity: annotation2142964
display id: annotation2142964
version: 1
top level: Biosurfact
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K653000/annotation2142964/range2142964/1

https://synbiohub.org/public/igem/BBa_K653000/annotation2142964/range2142964/1
type: Range
persistent identity: range2142964
display id: range2142964
version: 1
direction: direction/forward
top level: Biosurfact
start: 35
end: 922
orientation: inline

Silent Mutation
type: SequenceAnnotation
persistent identity: annotation2142965
display id: annotation2142965
version: 1
top level: Biosurfact
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_K653000/annotation2142965/range2142965/1

https://synbiohub.org/public/igem/BBa_K653000/annotation2142965/range2142965/1
type: Range
persistent identity: range2142965
display id: range2142965
version: 1
direction: direction/forward
top level: Biosurfact
start: 754
end: 759
orientation: inline

Silent Mutation
type: SequenceAnnotation
persistent identity: annotation2142967
display id: annotation2142967
version: 1
top level: Biosurfact
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_K653000/annotation2142967/range2142967/1

https://synbiohub.org/public/igem/BBa_K653000/annotation2142967/range2142967/1
type: Range
persistent identity: range2142967
display id: range2142967
version: 1
direction: direction/forward
top level: Biosurfact
start: 1777
end: 1782
orientation: inline

Rhamnosyltransferase B
type: SequenceAnnotation
persistent identity: annotation2142966
display id: annotation2142966
version: 1
top level: Biosurfact
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K653000/annotation2142966/range2142966/1

https://synbiohub.org/public/igem/BBa_K653000/annotation2142966/range2142966/1
type: Range
persistent identity: range2142966
display id: range2142966
version: 1
direction: direction/forward
top level: Biosurfact
start: 988
end: 2268
orientation: inline

Silent Mutation
type: SequenceAnnotation
persistent identity: annotation2142968
display id: annotation2142968
version: 1
top level: Biosurfact
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_K653000/annotation2142968/range2142968/1

https://synbiohub.org/public/igem/BBa_K653000/annotation2142968/range2142968/1
type: Range
persistent identity: range2142968
display id: range2142968
version: 1
direction: direction/forward
top level: Biosurfact
start: 1891
end: 1896
orientation: inline

Primer
type: SequenceAnnotation
persistent identity: annotation2142962
display id: annotation2142962
version: 1
top level: Biosurfact
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K653000/annotation2142962/range2142962/1

https://synbiohub.org/public/igem/BBa_K653000/annotation2142962/range2142962/1
type: Range
persistent identity: range2142962
display id: range2142962
version: 1
direction: direction/forward
top level: Biosurfact
start: 1
end: 19
orientation: inline

Primer
type: SequenceAnnotation
persistent identity: annotation2142969
display id: annotation2142969
version: 1
top level: Biosurfact
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K653000/annotation2142969/range2142969/1

https://synbiohub.org/public/igem/BBa_K653000/annotation2142969/range2142969/1
type: Range
persistent identity: range2142969
display id: range2142969
version: 1
direction: direction/reverse
top level: Biosurfact
start: 2296
end: 2314
orientation: inline

Rhamnosyltransferase gene complex 1
type: SequenceAnnotation
persistent identity: annotation2142963
display id: annotation2142963
version: 1
top level: Biosurfact
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K653000/annotation2142963/range2142963/1

https://synbiohub.org/public/igem/BBa_K653000/annotation2142963/range2142963/1
type: Range
persistent identity: range2142963
display id: range2142963
version: 1
direction: direction/forward
top level: Biosurfact
start: 35
end: 2234
orientation: inline

BBa_ K653000 RhlAB Panama
type: SequenceAnnotation
persistent identity: annotation2142961
display id: annotation2142961
version: 1
top level: Biosurfact
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K653000/annotation2142961/range2142961/1

https://synbiohub.org/public/igem/BBa_K653000/annotation2142961/range2142961/1
type: Range
persistent identity: range2142961
display id: range2142961
version: 1
direction: direction/forward
top level: Biosurfact
start: 1
end: 2314
orientation: inline

folKE
type: ComponentDefinition
persistent identity: BBa K137011
display id: BBa_K137011
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: folKE (GTP Cyclohydrolase I + pyrophosphokinase)
creator: Victoria Hsiao
created: 2008-06-19T11:00:00Z
modified: 2015-05-08T01:10:08Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _187_
m user id: 0
owner id: 2987
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: folKE is a bifunctional gene which encodes two necessary enzymes for folate biosynthesis (2-Amino-4-hydroxy-6-hydroxymethyldihydropteridine pyrophosphokinase / GTP cyclohydrolase I).
mutable notes: We were unsure of whether or not the L.lactis RBS would work with E. coli and so we are going to try cloning the entire gene cluster with the RBSs as well as cloning out each gene separately and adding E.coli RBSs.
mutable provenance: L.lactis genome
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K137011_sequence/1

BBa_K515100
type: ComponentDefinition
persistent identity: BBa K515100
display id: BBa_K515100
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: IAA biosynthetic genes under control of the Pveg2 promoter
creator: Atipat Patharagulpong
created: 2011-09-05T11:00:00Z
modified: 2015-05-08T01:12:32Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _680_
m user id: 0
owner id: 10179
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: The construct of IaaM and IaaH genes in PVEG plasmid
mutable notes: -
mutable provenance: genomics sequence
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K515001
component: BBa_K515010
component: BBa_K515000
sequence annotation: BBa_K515010
sequence annotation: BBa_K515000
sequence annotation: BBa_K515001
sequence: https://synbiohub.org/public/igem/BBa_K515100_sequence/1

BBa_K515001
type: Component
persistent identity: component2133838
display id: component2133838
version: 1
top level: BBa_K515100
access: public
definition: https://synbiohub.org/public/igem/BBa_K515001/1

BBa_K515010
type: Component
persistent identity: component2133830
display id: component2133830
version: 1
top level: BBa_K515100
access: public
definition: https://synbiohub.org/public/igem/BBa_K515010/1

BBa_K515000
type: Component
persistent identity: component2133834
display id: component2133834
version: 1
top level: BBa_K515100
access: public
definition: https://synbiohub.org/public/igem/BBa_K515000/1

BBa_K515010
type: SequenceAnnotation
persistent identity: annotation2133830
display id: annotation2133830
version: 1
top level: BBa_K515100
location: https://synbiohub.org/public/igem/BBa_K515100/annotation2133830/range2133830/1
component: BBa_K515010

https://synbiohub.org/public/igem/BBa_K515100/annotation2133830/range2133830/1
type: Range
persistent identity: range2133830
display id: range2133830
version: 1
direction: direction/reverse
top level: BBa_K515100
start: 1
end: 97
orientation: inline

BBa_K515000
type: SequenceAnnotation
persistent identity: annotation2133834
display id: annotation2133834
version: 1
top level: BBa_K515100
location: https://synbiohub.org/public/igem/BBa_K515100/annotation2133834/range2133834/1
component: BBa_K515000

https://synbiohub.org/public/igem/BBa_K515100/annotation2133834/range2133834/1
type: Range
persistent identity: range2133834
display id: range2133834
version: 1
direction: direction/reverse
top level: BBa_K515100
start: 98
end: 1783
orientation: inline

BBa_K515001
type: SequenceAnnotation
persistent identity: annotation2133838
display id: annotation2133838
version: 1
top level: BBa_K515100
location: https://synbiohub.org/public/igem/BBa_K515100/annotation2133838/range2133838/1
component: BBa_K515001

https://synbiohub.org/public/igem/BBa_K515100/annotation2133838/range2133838/1
type: Range
persistent identity: range2133838
display id: range2133838
version: 1
direction: direction/reverse
top level: BBa_K515100
start: 1784
end: 3165
orientation: inline

BBa_K137017
type: ComponentDefinition
persistent identity: BBa K137017
display id: BBa_K137017
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Galactose Oxidase
creator: Doug Tischer
created: 2008-07-14T11:00:00Z
modified: 2015-05-08T01:10:08Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _187_
m user id: 0
owner id: 3049
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Galactose oxidase is a fungal enzyme that uses molecular oxygen to oxidizes galactose and several other primary alcohols to the corresponding aldehyde, while also producing hydrogen peroxide. This particular galaxtose oxidase is not wild type. It has been optimized through directed evolution by the Arnold lab at CalTech to be functionally expressed in vivo in E. coli and to be thermostable. (Sun L, Petrounia IP, Yagasaki M, Bandara G, and Arnold FH. Expression and stabilization of galactose oxidase in Escherichia coli by directed evolution. Protein Eng 2001 Sep; 14(9) 699-704. pmid:11707617). This parts has undergone two rounds of site directed mutagenesis to remove one EcoRI and one PstI site. In our hands, it is functional towards galactose in vivo.
mutable notes: Two rounds of site directed mutagenesis had to be performed to eliminate an EcoRI and PstI site.
mutable provenance: Plasmid from the Arnold lab at CalTech, which is the result of their directed evolution to make galactose functionally expressed in E. coli and to improve its thermostability.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: A->G
sequence annotation: T->C
sequence: https://synbiohub.org/public/igem/BBa_K137017_sequence/1

A->G
type: SequenceAnnotation
persistent identity: annotation1967110
display id: annotation1967110
version: 1
top level: BBa_K137017
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K137017/annotation1967110/range1967110/1

https://synbiohub.org/public/igem/BBa_K137017/annotation1967110/range1967110/1
type: Range
persistent identity: range1967110
display id: range1967110
version: 1
direction: direction/forward
top level: BBa_K137017
start: 1386
end: 1386
orientation: inline

T->C
type: SequenceAnnotation
persistent identity: annotation1967109
display id: annotation1967109
version: 1
top level: BBa_K137017
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K137017/annotation1967109/range1967109/1

https://synbiohub.org/public/igem/BBa_K137017/annotation1967109/range1967109/1
type: Range
persistent identity: range1967109
display id: range1967109
version: 1
direction: direction/forward
top level: BBa_K137017
start: 522
end: 522
orientation: inline

rhlAB
type: ComponentDefinition
persistent identity: BBa K424018
display id: BBa_K424018
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Rhamnosiltransferase BioBrick (Rh1AB_BB)
creator: Team Panama iGEM 2010
created: 2010-10-10T11:00:00Z
modified: 2015-05-08T01:12:28Z
discontinued: false
dominant: false
experience: experience/None
group u list: _545_
m user id: 0
owner id: 7119
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: long description
mutable notes: illegal restriction enzymes lead us to apply multiple site mutagenesis technique.
mutable provenance: P. aeruginosa from INDICASAT and gene sequence reviewed in genbank.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
sequence annotation: end codon alanine
sequence annotation: silent mutation
sequence annotation: RhlA
sequence annotation: RhlB
sequence annotation: Rhamnosyltransferase complex
sequence annotation: start codon cysteine
sequence annotation: RhlAB Panama
sequence: https://synbiohub.org/public/igem/BBa_K424018_sequence/1

end codon alanine
type: SequenceAnnotation
persistent identity: annotation2086149
display id: annotation2086149
version: 1
top level: rhlAB
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K424018/annotation2086149/range2086149/1

https://synbiohub.org/public/igem/BBa_K424018/annotation2086149/range2086149/1
type: Range
persistent identity: range2086149
display id: range2086149
version: 1
direction: direction/forward
top level: rhlAB
start: 2432
end: 2434
orientation: inline

silent mutation
type: SequenceAnnotation
persistent identity: annotation2086146
display id: annotation2086146
version: 1
top level: rhlAB
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_K424018/annotation2086146/range2086146/1

https://synbiohub.org/public/igem/BBa_K424018/annotation2086146/range2086146/1
type: Range
persistent identity: range2086146
display id: range2086146
version: 1
direction: direction/forward
top level: rhlAB
start: 1843
end: 1848
orientation: inline

RhlA
type: SequenceAnnotation
persistent identity: annotation2086143
display id: annotation2086143
version: 1
top level: rhlAB
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K424018/annotation2086143/range2086143/1

https://synbiohub.org/public/igem/BBa_K424018/annotation2086143/range2086143/1
type: Range
persistent identity: range2086143
display id: range2086143
version: 1
direction: direction/forward
top level: rhlAB
start: 101
end: 888
orientation: inline

RhlB
type: SequenceAnnotation
persistent identity: annotation2086144
display id: annotation2086144
version: 1
top level: rhlAB
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K424018/annotation2086144/range2086144/1

https://synbiohub.org/public/igem/BBa_K424018/annotation2086144/range2086144/1
type: Range
persistent identity: range2086144
display id: range2086144
version: 1
direction: direction/forward
top level: rhlAB
start: 1054
end: 2334
orientation: inline

silent mutation
type: SequenceAnnotation
persistent identity: annotation2086147
display id: annotation2086147
version: 1
top level: rhlAB
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_K424018/annotation2086147/range2086147/1

https://synbiohub.org/public/igem/BBa_K424018/annotation2086147/range2086147/1
type: Range
persistent identity: range2086147
display id: range2086147
version: 1
direction: direction/forward
top level: rhlAB
start: 1957
end: 1962
orientation: inline

Rhamnosyltransferase complex
type: SequenceAnnotation
persistent identity: annotation2086180
display id: annotation2086180
version: 1
top level: rhlAB
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K424018/annotation2086180/range2086180/1

https://synbiohub.org/public/igem/BBa_K424018/annotation2086180/range2086180/1
type: Range
persistent identity: range2086180
display id: range2086180
version: 1
direction: direction/forward
top level: rhlAB
start: 1
end: 2434
orientation: inline

silent mutation
type: SequenceAnnotation
persistent identity: annotation2086145
display id: annotation2086145
version: 1
top level: rhlAB
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_K424018/annotation2086145/range2086145/1

https://synbiohub.org/public/igem/BBa_K424018/annotation2086145/range2086145/1
type: Range
persistent identity: range2086145
display id: range2086145
version: 1
direction: direction/forward
top level: rhlAB
start: 820
end: 825
orientation: inline

start codon cysteine
type: SequenceAnnotation
persistent identity: annotation2086148
display id: annotation2086148
version: 1
top level: rhlAB
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K424018/annotation2086148/range2086148/1

https://synbiohub.org/public/igem/BBa_K424018/annotation2086148/range2086148/1
type: Range
persistent identity: range2086148
display id: range2086148
version: 1
direction: direction/forward
top level: rhlAB
start: 1
end: 3
orientation: inline

RhlAB Panama
type: SequenceAnnotation
persistent identity: annotation2086159
display id: annotation2086159
version: 1
top level: rhlAB
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K424018/annotation2086159/range2086159/1

https://synbiohub.org/public/igem/BBa_K424018/annotation2086159/range2086159/1
type: Range
persistent identity: range2086159
display id: range2086159
version: 1
direction: direction/forward
top level: rhlAB
start: 101
end: 2334
orientation: inline

BBa_K108027
type: ComponentDefinition
persistent identity: BBa K108027
display id: BBa_K108027
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: XylM
creator: Qi Liu
created: 2008-10-22T11:00:00Z
modified: 2015-05-08T01:09:04Z
discontinued: false
dominant: false
experience: experience/None
group u list: _222_
m user id: 0
owner id: 2671
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: coming
mutable notes: PCR using primers with biobrick prefix and surfix, and then cloned into pSB1AC3.
mutable provenance: pWW0
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: XylM
sequence: https://synbiohub.org/public/igem/BBa_K108027_sequence/1

XylM
type: SequenceAnnotation
persistent identity: annotation1986982
display id: annotation1986982
version: 1
top level: BBa_K108027
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K108027/annotation1986982/range1986982/1

https://synbiohub.org/public/igem/BBa_K108027/annotation1986982/range1986982/1
type: Range
persistent identity: range1986982
display id: range1986982
version: 1
direction: direction/forward
top level: BBa_K108027
start: 1
end: 1107
orientation: inline

aspA
type: ComponentDefinition
persistent identity: BBa C0083
display id: BBa_C0083
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: aspartate ammonia-lyase
creator: Stephen Lee, Roshan Kumar, Joe Levine, Ziyan Chu (Polkadorks, IAP 2004)
created: 2004-01-27T12:00:00Z
modified: 2015-08-31T04:07:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Coding sequence for AspA enzyme. AspA aminates fumarate to make aspartate. Aspartate can be used as a bacterial chemotaxis signal.
mutable notes: The start codon was changed from GTG to ATG to match BioBricks standards. Five silent mutations were introduced to eliminate EcoRI and PstI restriction enzyme sites. EcoRI sites were eliminated at bases 588 and 657 with C -> T mutations. PstI sites were eliminated at bases 624, 999, and 1125 with G -> A mutations. The mutations were designed to match codons with similar frequencies of usage in E. coli.
mutable provenance: AE000486 E. coli K12
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: AspA
sequence annotation: G
sequence annotation: LVA
sequence annotation: Help:Barcodes
sequence annotation: C
sequence: https://synbiohub.org/public/igem/BBa_C0083_sequence/1

AspA
type: SequenceAnnotation
persistent identity: annotation300981
display id: annotation300981
version: 1
top level: aspA
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_C0083/annotation300981/range300981/1

https://synbiohub.org/public/igem/BBa_C0083/annotation300981/range300981/1
type: Range
persistent identity: range300981
display id: range300981
version: 1
direction: direction/forward
top level: aspA
start: 1
end: 1479
orientation: inline

G
type: SequenceAnnotation
persistent identity: annotation300984
display id: annotation300984
version: 1
top level: aspA
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_C0083/annotation300984/range300984/1

https://synbiohub.org/public/igem/BBa_C0083/annotation300984/range300984/1
type: Range
persistent identity: range300984
display id: range300984
version: 1
direction: direction/forward
top level: aspA
start: 624
end: 624
orientation: inline

G
type: SequenceAnnotation
persistent identity: annotation300987
display id: annotation300987
version: 1
top level: aspA
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_C0083/annotation300987/range300987/1

https://synbiohub.org/public/igem/BBa_C0083/annotation300987/range300987/1
type: Range
persistent identity: range300987
display id: range300987
version: 1
direction: direction/forward
top level: aspA
start: 999
end: 999
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation300993
display id: annotation300993
version: 1
top level: aspA
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_C0083/annotation300993/range300993/1

https://synbiohub.org/public/igem/BBa_C0083/annotation300993/range300993/1
type: Range
persistent identity: range300993
display id: range300993
version: 1
direction: direction/forward
top level: aspA
start: 1480
end: 1512
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213998
display id: annotation2213998
version: 1
top level: aspA
role: SO:0000807
role: feature/barcode
location: https://synbiohub.org/public/igem/BBa_C0083/annotation2213998/range2213998/1

https://synbiohub.org/public/igem/BBa_C0083/annotation2213998/range2213998/1
type: Range
persistent identity: range2213998
display id: range2213998
version: 1
direction: direction/forward
top level: aspA
start: 1519
end: 1543
orientation: inline

C
type: SequenceAnnotation
persistent identity: annotation300983
display id: annotation300983
version: 1
top level: aspA
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_C0083/annotation300983/range300983/1

https://synbiohub.org/public/igem/BBa_C0083/annotation300983/range300983/1
type: Range
persistent identity: range300983
display id: range300983
version: 1
direction: direction/forward
top level: aspA
start: 588
end: 588
orientation: inline

G
type: SequenceAnnotation
persistent identity: annotation300982
display id: annotation300982
version: 1
top level: aspA
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_C0083/annotation300982/range300982/1

https://synbiohub.org/public/igem/BBa_C0083/annotation300982/range300982/1
type: Range
persistent identity: range300982
display id: range300982
version: 1
direction: direction/forward
top level: aspA
start: 1
end: 1
orientation: inline

G
type: SequenceAnnotation
persistent identity: annotation300988
display id: annotation300988
version: 1
top level: aspA
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_C0083/annotation300988/range300988/1

https://synbiohub.org/public/igem/BBa_C0083/annotation300988/range300988/1
type: Range
persistent identity: range300988
display id: range300988
version: 1
direction: direction/forward
top level: aspA
start: 1125
end: 1125
orientation: inline

C
type: SequenceAnnotation
persistent identity: annotation300986
display id: annotation300986
version: 1
top level: aspA
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_C0083/annotation300986/range300986/1

https://synbiohub.org/public/igem/BBa_C0083/annotation300986/range300986/1
type: Range
persistent identity: range300986
display id: range300986
version: 1
direction: direction/forward
top level: aspA
start: 657
end: 657
orientation: inline

BBa_I737020
type: ComponentDefinition
persistent identity: BBa I737020
display id: BBa_I737020
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PoPS->Agarase (Digests agar)
creator: Vladislav Lavrovsky
created: 2007-10-25T11:00:00Z
modified: 2015-08-31T04:08:01Z
discontinued: false
dominant: false
experience: experience/None
group u list: _114_
m user id: 0
owner id: 905
owning group id: 77
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This part is Beta-Agarase cloned out of Pseudoalteromonas atlantica T6C from the ATCC. Pseudoalteromonas atlantica is a gram-negative, motile, chemoorganotroph which lives in sea water. We made a series of primers and cloned agarase using a TOPO cloning kit. Currently the gene has a EcorRI site which limits how it can be assembled.
mutable notes: There is a EcoRI site in the sequence which must be mutated at some point.
mutable provenance: Cloned out of Pseudoalteromonas atlantica T6C from the ATCC.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
sequence annotation: Agarase
sequence annotation: Stop
sequence annotation: Reverse Primer
sequence annotation: Forward Primer
sequence annotation: Start
sequence: https://synbiohub.org/public/igem/BBa_I737020_sequence/1

Agarase
type: SequenceAnnotation
persistent identity: annotation1955819
display id: annotation1955819
version: 1
top level: BBa_I737020
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_I737020/annotation1955819/range1955819/1

https://synbiohub.org/public/igem/BBa_I737020/annotation1955819/range1955819/1
type: Range
persistent identity: range1955819
display id: range1955819
version: 1
direction: direction/forward
top level: BBa_I737020
start: 53
end: 2495
orientation: inline

Stop
type: SequenceAnnotation
persistent identity: annotation1957509
display id: annotation1957509
version: 1
top level: BBa_I737020
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_I737020/annotation1957509/range1957509/1

https://synbiohub.org/public/igem/BBa_I737020/annotation1957509/range1957509/1
type: Range
persistent identity: range1957509
display id: range1957509
version: 1
direction: direction/forward
top level: BBa_I737020
start: 2493
end: 2495
orientation: inline

Reverse Primer
type: SequenceAnnotation
persistent identity: annotation1957377
display id: annotation1957377
version: 1
top level: BBa_I737020
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_I737020/annotation1957377/range1957377/1

https://synbiohub.org/public/igem/BBa_I737020/annotation1957377/range1957377/1
type: Range
persistent identity: range1957377
display id: range1957377
version: 1
direction: direction/reverse
top level: BBa_I737020
start: 2571
end: 2591
orientation: inline

Forward Primer
type: SequenceAnnotation
persistent identity: annotation1957376
display id: annotation1957376
version: 1
top level: BBa_I737020
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_I737020/annotation1957376/range1957376/1

https://synbiohub.org/public/igem/BBa_I737020/annotation1957376/range1957376/1
type: Range
persistent identity: range1957376
display id: range1957376
version: 1
direction: direction/forward
top level: BBa_I737020
start: 1
end: 20
orientation: inline

Start
type: SequenceAnnotation
persistent identity: annotation1957508
display id: annotation1957508
version: 1
top level: BBa_I737020
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_I737020/annotation1957508/range1957508/1

https://synbiohub.org/public/igem/BBa_I737020/annotation1957508/range1957508/1
type: Range
persistent identity: range1957508
display id: range1957508
version: 1
direction: direction/forward
top level: BBa_I737020
start: 53
end: 56
orientation: inline

BBa_K1554001
type: ComponentDefinition
persistent identity: BBa K1554001
display id: BBa_K1554001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: AtrΔ11
creator: Valencia_UPV team members
created: 2014-09-25T11:00:00Z
modified: 2015-05-28T11:28:57Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1939_
m user id: 4206
owner id: 22386
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: Delta11 desaturase
mutable notes: BioBrick compatible
mutable provenance: It comes from Amyelois transitella.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1554001_sequence/1

BBa_K118018
type: ComponentDefinition
persistent identity: BBa K118018
display id: BBa_K118018
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: rbs+glgC16 (glgC with G336D substitution)
creator: Andrew Hall
created: 2008-10-05T11:00:00Z
modified: 2015-05-08T01:09:37Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _192_
m user id: 0
owner id: 3282
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: This is the coding sequence of ''glgC'' (ADP-glucose pyrophosphorylase) from ''Escherichia coli'' JM109 with the substitution G336D, with added ribosome binding site (rbs). This mutation is known to cause increased activity of ADP-glucose pyrophosphorylase in the absence of the activator fructose 1,6-bisphosphate (FBP), high affinity for FBP and substrates lower affinity for the inhibitor AMP. (Leung ''et al.'', 1986; Meyer ''et al.'', 1988)
mutable notes: No special considerations
mutable provenance: ''Escherichia coli'' JM109 genomic DNA.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_K118016
component: BBa_J15001
sequence annotation: BBa_J15001
sequence annotation: BBa_K118016
sequence: https://synbiohub.org/public/igem/BBa_K118018_sequence/1

BBa_K118016
type: Component
persistent identity: component1978637
display id: component1978637
version: 1
top level: BBa_K118018
access: public
definition: BBa_K118016

BBa_J15001
type: Component
persistent identity: component1978633
display id: component1978633
version: 1
top level: BBa_K118018
access: public
definition: https://synbiohub.org/public/igem/BBa_J15001/1

BBa_J15001
type: SequenceAnnotation
persistent identity: annotation1978633
display id: annotation1978633
version: 1
top level: BBa_K118018
location: https://synbiohub.org/public/igem/BBa_K118018/annotation1978633/range1978633/1
component: BBa_J15001

https://synbiohub.org/public/igem/BBa_K118018/annotation1978633/range1978633/1
type: Range
persistent identity: range1978633
display id: range1978633
version: 1
direction: direction/reverse
top level: BBa_K118018
start: 1
end: 10
orientation: inline

BBa_K118016
type: SequenceAnnotation
persistent identity: annotation1978637
display id: annotation1978637
version: 1
top level: BBa_K118018
location: https://synbiohub.org/public/igem/BBa_K118018/annotation1978637/range1978637/1
component: BBa_K118016

https://synbiohub.org/public/igem/BBa_K118018/annotation1978637/range1978637/1
type: Range
persistent identity: range1978637
display id: range1978637
version: 1
direction: direction/reverse
top level: BBa_K118018
start: 17
end: 1315
orientation: inline

ACCoxidase
type: ComponentDefinition
persistent identity: BBa K417002
display id: BBa_K417002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: 1-aminocyclopropane-1-carboxylate (ACC) oxidase
creator: Ben Porebski, Andrew Perry
created: 2010-10-19T11:00:00Z
modified: 2015-05-08T01:12:28Z
discontinued: false
dominant: false
experience: experience/None
group u list: _537_
m user id: 0
owner id: 6513
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is the protein coding sequence of 1-aminocyclopropane-1-carboxylate (ACC) oxidase, which catalyses the formation of ethylene from 1-aminocyclopropane-1-carboxylate under the presence of ascorbate and O2. (1-aminocyclopropane-1-carboxylate + ascorbate + O2 = ethylene + cyanide + dehydroascorbate + CO2 + 2 H2O) This enzyme is commonly referred to as ACC oxidase or ethylene forming enzyme (EFE).
mutable notes: Codon optimization for E. coli and removal of biobrick restriction sites.
mutable provenance: Solanum lycopersicum (Tomato) (Lycopersicon esculentum) codon optimized for E. coli.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Start
sequence annotation: ACC Oxidase
sequence annotation: Stop
sequence: https://synbiohub.org/public/igem/BBa_K417002_sequence/1

Start
type: SequenceAnnotation
persistent identity: annotation2090378
display id: annotation2090378
version: 1
top level: ACCoxidase
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K417002/annotation2090378/range2090378/1

https://synbiohub.org/public/igem/BBa_K417002/annotation2090378/range2090378/1
type: Range
persistent identity: range2090378
display id: range2090378
version: 1
direction: direction/forward
top level: ACCoxidase
start: 1
end: 3
orientation: inline

ACC Oxidase
type: SequenceAnnotation
persistent identity: annotation2090379
display id: annotation2090379
version: 1
top level: ACCoxidase
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K417002/annotation2090379/range2090379/1

https://synbiohub.org/public/igem/BBa_K417002/annotation2090379/range2090379/1
type: Range
persistent identity: range2090379
display id: range2090379
version: 1
direction: direction/forward
top level: ACCoxidase
start: 1
end: 948
orientation: inline

Stop
type: SequenceAnnotation
persistent identity: annotation2090380
display id: annotation2090380
version: 1
top level: ACCoxidase
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K417002/annotation2090380/range2090380/1

https://synbiohub.org/public/igem/BBa_K417002/annotation2090380/range2090380/1
type: Range
persistent identity: range2090380
display id: range2090380
version: 1
direction: direction/forward
top level: ACCoxidase
start: 946
end: 948
orientation: inline

rbs+LIMS1
type: ComponentDefinition
persistent identity: BBa I742111
display id: BBa_I742111
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Limonene synthase gene with ribosome binding site
creator: sarah hollingshead
created: 2007-10-18T11:00:00Z
modified: 2015-08-31T04:08:02Z
discontinued: false
dominant: false
experience: experience/None
group u list: _123_
m user id: 0
owner id: 1964
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: Ribosome binding site attached to Limonene synthase gene. Limonene synthase enzyme produces (+)-limonene from farnesyl diphosphate (intermediate in mevlonate pathway). (+) Limonene is a component of lemon scent.
mutable notes: several codons were altered silently to remove several forbidden restriction sites. The N-terminal region (plastid targeting sequence) was removed, removing the first 51 amino acids and replacing codon 52 with the start codon ATG. LIMS cDNA was used as genomic DNA contains introns.
mutable provenance: Monoterpene biosynthesis in lemon (Citrus limon) cDNA isolation and functional analysis of four monoterpene synthases, J Lucker, et al, Eur. J. Biochem. 269: 3160???3171 (2002) Analysis of Lemon and Bergamot Essential Oils by HPLC with Microbore Columns, M Benincasa, et al, Chromatographia 30(5/6):271 (1990) Citrus fruit flavour and aroma biosynthesis, L Sharon-Asa, The Plant Journal, 36:664 (2003)
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_J15001
component: BBa_I742110
sequence annotation: BBa_J15001
sequence annotation: BBa_I742110
sequence: https://synbiohub.org/public/igem/BBa_I742111_sequence/1

BBa_J15001
type: Component
persistent identity: component1955370
display id: component1955370
version: 1
top level: rbs+LIMS1
access: public
definition: https://synbiohub.org/public/igem/BBa_J15001/1

BBa_I742110
type: Component
persistent identity: component1955374
display id: component1955374
version: 1
top level: rbs+LIMS1
access: public
definition: https://synbiohub.org/public/igem/BBa_I742110/1

BBa_J15001
type: SequenceAnnotation
persistent identity: annotation1955370
display id: annotation1955370
version: 1
top level: rbs+LIMS1
location: https://synbiohub.org/public/igem/BBa_I742111/annotation1955370/range1955370/1
component: BBa_J15001

https://synbiohub.org/public/igem/BBa_I742111/annotation1955370/range1955370/1
type: Range
persistent identity: range1955370
display id: range1955370
version: 1
direction: direction/reverse
top level: rbs+LIMS1
start: 1
end: 10
orientation: inline

BBa_I742110
type: SequenceAnnotation
persistent identity: annotation1955374
display id: annotation1955374
version: 1
top level: rbs+LIMS1
location: https://synbiohub.org/public/igem/BBa_I742111/annotation1955374/range1955374/1
component: BBa_I742110

https://synbiohub.org/public/igem/BBa_I742111/annotation1955374/range1955374/1
type: Range
persistent identity: range1955374
display id: range1955374
version: 1
direction: direction/reverse
top level: rbs+LIMS1
start: 17
end: 1687
orientation: inline

BBa_K147003
type: ComponentDefinition
persistent identity: BBa K147003
display id: BBa_K147003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: ohbA
creator: Nathan Puhl
created: 2008-10-27T12:00:00Z
modified: 2015-05-08T01:10:39Z
discontinued: false
dominant: false
group u list: _129_242_
m user id: 0
owner id: 1757
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Blah Blah Blah
mutable notes: Blah Blah Blah
mutable provenance: Blah Blah Blah
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K147003_sequence/1

metK
type: ComponentDefinition
persistent identity: BBa K1668001
display id: BBa_K1668001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: metK (from Streptomyces avermitilis, increasing avermectin production)
creator: iGEM15_ZJU-China
created: 2015-08-13T11:00:00Z
modified: 2015-09-13T01:40:17Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2086_
m user id: 24510
owner id: 24510
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: coding sequence
mutable notes: overlap
mutable provenance: genomic sequence
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: stop coden
sequence annotation: cds
sequence annotation: start coden
sequence: https://synbiohub.org/public/igem/BBa_K1668001_sequence/1

stop coden
type: SequenceAnnotation
persistent identity: annotation2447011
display id: annotation2447011
version: 1
top level: metK
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1668001/annotation2447011/range2447011/1

https://synbiohub.org/public/igem/BBa_K1668001/annotation2447011/range2447011/1
type: Range
persistent identity: range2447011
display id: range2447011
version: 1
direction: direction/forward
top level: metK
start: 811
end: 816
orientation: inline

cds
type: SequenceAnnotation
persistent identity: annotation2456726
display id: annotation2456726
version: 1
top level: metK
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1668001/annotation2456726/range2456726/1

https://synbiohub.org/public/igem/BBa_K1668001/annotation2456726/range2456726/1
type: Range
persistent identity: range2456726
display id: range2456726
version: 1
direction: direction/forward
top level: metK
start: 1
end: 816
orientation: inline

start coden
type: SequenceAnnotation
persistent identity: annotation2447010
display id: annotation2447010
version: 1
top level: metK
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1668001/annotation2447010/range2447010/1

https://synbiohub.org/public/igem/BBa_K1668001/annotation2447010/range2447010/1
type: Range
persistent identity: range2447010
display id: range2447010
version: 1
direction: direction/forward
top level: metK
start: 1
end: 3
orientation: inline

BBa_K727610
type: ComponentDefinition
persistent identity: BBa K727610
display id: BBa_K727610
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Rose Odor Generator (Geraniol)
creator: İBRAHİM YASİR ORHAN
created: 2012-06-15T11:00:00Z
modified: 2015-05-08T01:13:05Z
discontinued: true
dominant: false
experience: experience/None
group u list: _973_
m user id: 0
owner id: 11153
owning group id: 9
sample status: Discontinued
status: status/Deleted
bookmark: false
mutable description: This part is assembled with geranyl diphosphate synthase (BBa_K727005) and geraniol synthase (BBa_K727006) in order to produce geraniol which generates rose scent. Geraniol is a monoterpenoid and an alcohol. It???s molecular formula is C10H18O . It is the primary part of rose oil, palmarosa oil, and citronella oil (Java type). It also occurs in small quantities in geranium, lemon, and many other essential oils. It has a rose-like odor and is commonly used in perfumes. It is used in flavors such as peach, raspberry, grapefruit, red apple, plum, lime, orange, lemon, watermelon, pineapple, and blueberry.
mutable notes: Optimized for E.coli
mutable provenance: Genome of
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K727005
component: BBa_K727006
component: BBa_K727007
component: BBa_R0062
sequence annotation: BBa_R0062
sequence annotation: BBa_K727006
sequence annotation: BBa_K727005
sequence annotation: BBa_K727007
sequence: https://synbiohub.org/public/igem/BBa_K727610_sequence/1

BBa_K727005
type: Component
persistent identity: component2176453
display id: component2176453
version: 1
top level: BBa_K727610
access: public
definition: https://synbiohub.org/public/igem/BBa_K727005/1

BBa_K727006
type: Component
persistent identity: component2176457
display id: component2176457
version: 1
top level: BBa_K727610
access: public
definition: https://synbiohub.org/public/igem/BBa_K727006/1

BBa_K727007
type: Component
persistent identity: component2176455
display id: component2176455
version: 1
top level: BBa_K727610
access: public
definition: https://synbiohub.org/public/igem/BBa_K727007/1

BBa_R0062
type: Component
persistent identity: component2176448
display id: component2176448
version: 1
top level: BBa_K727610
access: public
definition: https://synbiohub.org/public/igem/BBa_R0062/1

BBa_R0062
type: SequenceAnnotation
persistent identity: annotation2176448
display id: annotation2176448
version: 1
top level: BBa_K727610
location: https://synbiohub.org/public/igem/BBa_K727610/annotation2176448/range2176448/1
component: BBa_R0062

https://synbiohub.org/public/igem/BBa_K727610/annotation2176448/range2176448/1
type: Range
persistent identity: range2176448
display id: range2176448
version: 1
direction: direction/forward
top level: BBa_K727610
start: 1
end: 55
orientation: inline

BBa_K727006
type: SequenceAnnotation
persistent identity: annotation2176457
display id: annotation2176457
version: 1
top level: BBa_K727610
location: https://synbiohub.org/public/igem/BBa_K727610/annotation2176457/range2176457/1
component: BBa_K727006

https://synbiohub.org/public/igem/BBa_K727610/annotation2176457/range2176457/1
type: Range
persistent identity: range2176457
display id: range2176457
version: 1
direction: direction/reverse
top level: BBa_K727610
start: 1013
end: 2862
orientation: inline

BBa_K727005
type: SequenceAnnotation
persistent identity: annotation2176453
display id: annotation2176453
version: 1
top level: BBa_K727610
location: https://synbiohub.org/public/igem/BBa_K727610/annotation2176453/range2176453/1
component: BBa_K727005

https://synbiohub.org/public/igem/BBa_K727610/annotation2176453/range2176453/1
type: Range
persistent identity: range2176453
display id: range2176453
version: 1
direction: direction/reverse
top level: BBa_K727610
start: 64
end: 1004
orientation: inline

BBa_K727007
type: SequenceAnnotation
persistent identity: annotation2176455
display id: annotation2176455
version: 1
top level: BBa_K727610
location: https://synbiohub.org/public/igem/BBa_K727610/annotation2176455/range2176455/1
component: BBa_K727007

https://synbiohub.org/public/igem/BBa_K727610/annotation2176455/range2176455/1
type: Range
persistent identity: range2176455
display id: range2176455
version: 1
direction: direction/reverse
top level: BBa_K727610
start: 1
end: 1004
orientation: inline

BBa_K081017
type: ComponentDefinition
persistent identity: BBa K081017
display id: BBa_K081017
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pcyA protein generator (TERM-)
creator: Lorenzo Pasotti, Paolo Magni
created: 2008-10-18T11:00:00Z
modified: 2015-05-08T01:08:35Z
discontinued: false
dominant: true
experience: experience/None
group u list: _227_
m user id: 0
owner id: 2583
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: pcyA converts biliverdin IXalpha (BV) to phycocyanobilin (PCB). Strong RBS (efficiency=0.6).
mutable notes: We used BioBrick Standard Assembly.
mutable provenance: RBS: pcyA:
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_I15009
component: BBa_B0030
sequence annotation: BBa_B0030
sequence annotation: BBa_I15009
sequence: https://synbiohub.org/public/igem/BBa_K081017_sequence/1

BBa_I15009
type: Component
persistent identity: component2244006
display id: component2244006
version: 1
top level: BBa_K081017
access: public
definition: https://synbiohub.org/public/igem/BBa_I15009/1

BBa_B0030
type: Component
persistent identity: component2244002
display id: component2244002
version: 1
top level: BBa_K081017
access: public
definition: https://synbiohub.org/public/igem/BBa_B0030/1

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation2244002
display id: annotation2244002
version: 1
top level: BBa_K081017
location: https://synbiohub.org/public/igem/BBa_K081017/annotation2244002/range2244002/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_K081017/annotation2244002/range2244002/1
type: Range
persistent identity: range2244002
display id: range2244002
version: 1
direction: direction/forward
top level: BBa_K081017
start: 1
end: 15
orientation: inline

BBa_I15009
type: SequenceAnnotation
persistent identity: annotation2244006
display id: annotation2244006
version: 1
top level: BBa_K081017
location: https://synbiohub.org/public/igem/BBa_K081017/annotation2244006/range2244006/1
component: BBa_I15009

https://synbiohub.org/public/igem/BBa_K081017/annotation2244006/range2244006/1
type: Range
persistent identity: range2244006
display id: range2244006
version: 1
direction: direction/reverse
top level: BBa_K081017
start: 22
end: 796
orientation: inline

GluColi
type: ComponentDefinition
persistent identity: BBa K196014
display id: BBa_K196014
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Final GluColi device.
creator: Laetitia Warny
created: 2009-10-17T11:00:00Z
modified: 2015-05-08T01:11:17Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _309_
m user id: 0
owner id: 4813
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This is the part we actually created to test the glue production by E. coli. HfsG and HfsH come from Caulobacter crescentu. Caulobacter crescentus is an aquatic, Gram-negative bacterium that divides asymmetrically and is able to synthetize a strong glue. This glue is mainly made of a polysaccharide. There are different proteins needed to synthetize, export and attach it to the stalk of Caulobacter. To see the hole system, please see our wiki page [1]. In our project, we would like this glue to be produced by Escherichia coli. As E. coli does have homolog genes for many of these proteins, but not for HfsG and HfsH, we decided to create a plasmid including only the genes coding for these two proteins. HfsG is a glycosyltransferase and HfsH is a carbohydrate esterase. RFP has been added as a reporter.
mutable notes: As many mutations were needed to make the part compatible with the standard 10, we decided to make hfsG and hfsH synthetized by GeneArt [2]. We also optimized it for E. coli. We mean that the codons were changed to favour the most present in E. coli.
mutable provenance: HfsG and HfsH sequences come from Caulobacter crescentus.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_K196007
component: BBa_K196005
sequence annotation: BBa_K196007
sequence annotation: BBa_K196005
sequence: https://synbiohub.org/public/igem/BBa_K196014_sequence/1

BBa_K196007
type: Component
persistent identity: component2267879
display id: component2267879
version: 1
top level: GluColi
access: public
definition: https://synbiohub.org/public/igem/BBa_K196007/1

BBa_K196005
type: Component
persistent identity: component2267888
display id: component2267888
version: 1
top level: GluColi
access: public
definition: https://synbiohub.org/public/igem/BBa_K196005/1

BBa_K196007
type: SequenceAnnotation
persistent identity: annotation2267879
display id: annotation2267879
version: 1
top level: GluColi
location: https://synbiohub.org/public/igem/BBa_K196014/annotation2267879/range2267879/1
component: BBa_K196007

https://synbiohub.org/public/igem/BBa_K196014/annotation2267879/range2267879/1
type: Range
persistent identity: range2267879
display id: range2267879
version: 1
direction: direction/reverse
top level: GluColi
start: 1
end: 787
orientation: inline

BBa_K196005
type: SequenceAnnotation
persistent identity: annotation2267888
display id: annotation2267888
version: 1
top level: GluColi
location: https://synbiohub.org/public/igem/BBa_K196014/annotation2267888/range2267888/1
component: BBa_K196005

https://synbiohub.org/public/igem/BBa_K196014/annotation2267888/range2267888/1
type: Range
persistent identity: range2267888
display id: range2267888
version: 1
direction: direction/reverse
top level: GluColi
start: 794
end: 2646
orientation: inline

BBa_K118016
type: ComponentDefinition
persistent identity: BBa K118016
display id: BBa_K118016
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: glgC16 (glgC with G336D substitution)
creator: Andrew Hall
created: 2008-10-05T11:00:00Z
modified: 2015-05-08T01:09:37Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _192_
m user id: 0
owner id: 3282
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: This is the coding sequence of ''glgC'' (ADP-glucose pyrophosphorylase) from ''Escherichia coli'' JM109 with the substitution G336D. This mutation is known to cause increased activity of ADP-glucose pyrophosphorylase in the absence of the activator fructose 1,6-bisphosphate (FBP), high affinity for FBP and substrates lower affinity for the inhibitor AMP. (Leung ''et al'', 1986; Meyer ''et al.'', 1988)
mutable notes: The mutation G336D was achieved by the substitution of A for G at position 1076 (codon GGC->GAC) by MABEL.
mutable provenance: ''Escherichia coli'' JM109 genomic DNA
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: EcoRI site removed
sequence annotation: G336D
sequence: https://synbiohub.org/public/igem/BBa_K118016_sequence/1

EcoRI site removed
type: SequenceAnnotation
persistent identity: annotation1978558
display id: annotation1978558
version: 1
top level: BBa_K118016
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_K118016/annotation1978558/range1978558/1

https://synbiohub.org/public/igem/BBa_K118016/annotation1978558/range1978558/1
type: Range
persistent identity: range1978558
display id: range1978558
version: 1
direction: direction/forward
top level: BBa_K118016
start: 571
end: 576
orientation: inline

G336D
type: SequenceAnnotation
persistent identity: annotation1978557
display id: annotation1978557
version: 1
top level: BBa_K118016
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K118016/annotation1978557/range1978557/1

https://synbiohub.org/public/igem/BBa_K118016/annotation1978557/range1978557/1
type: Range
persistent identity: range1978557
display id: range1978557
version: 1
direction: direction/forward
top level: BBa_K118016
start: 1006
end: 1008
orientation: inline

EcoRI site removed
type: SequenceAnnotation
persistent identity: annotation1978559
display id: annotation1978559
version: 1
top level: BBa_K118016
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_K118016/annotation1978559/range1978559/1

https://synbiohub.org/public/igem/BBa_K118016/annotation1978559/range1978559/1
type: Range
persistent identity: range1978559
display id: range1978559
version: 1
direction: direction/forward
top level: BBa_K118016
start: 1068
end: 1073
orientation: inline

BBa_K108028
type: ComponentDefinition
persistent identity: BBa K108028
display id: BBa_K108028
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: XylB
creator: Qi Liu
created: 2008-10-22T11:00:00Z
modified: 2015-05-08T01:09:04Z
discontinued: false
dominant: false
experience: experience/None
group u list: _222_
m user id: 0
owner id: 2671
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: coming
mutable notes: PCR using primers with biobrick prefix and surfix, and then cloned into pSB1AC3.
mutable provenance: pWW0
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: XylB
sequence: https://synbiohub.org/public/igem/BBa_K108028_sequence/1

XylB
type: SequenceAnnotation
persistent identity: annotation1986983
display id: annotation1986983
version: 1
top level: BBa_K108028
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K108028/annotation1986983/range1986983/1

https://synbiohub.org/public/igem/BBa_K108028/annotation1986983/range1986983/1
type: Range
persistent identity: range1986983
display id: range1986983
version: 1
direction: direction/forward
top level: BBa_K108028
start: 1
end: 1098
orientation: inline

BBa_K1919500
type: ComponentDefinition
persistent identity: BBa K1919500
display id: BBa_K1919500
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: K1321200+J04450 (Vitreoscilla hemoglobin + mRFP)
creator: Hongbin Yu
created: 2016-09-11T11:00:00Z
modified: 2016-10-14T07:59:48Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2386_
m user id: 26101
owner id: 26101
owning group id: 9
status: status/Planning
bookmark: false
mutable description: To measure the characters of Vitreoscilla haemoglobin generator constructed by iGEM14_Imperial (BBa_K1321200). Vitreoscilla haemoglobin can promote the cell growth and protein production ability. We ligate mRFP generator with VHb generator to test the protein production ability in E. coli.
mutable notes: The Vitreoscilla haemoglobin gene sequence in iGEM database is not as the same as that in Genebank but it works.
mutable provenance: iGEM HQ.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
sequence annotation: BBa_K1321200
sequence annotation: BBa_B0012
sequence annotation: J23101 constitutive
sequence annotation: Stop
sequence annotation: CAP
sequence annotation: polya
sequence annotation: BBa_E1010
sequence annotation: lac operator
sequence annotation: BBa_R0010
sequence annotation: T7 TE
sequence annotation: mrfp1
sequence annotation: VHb
sequence annotation: BBa_J04450
sequence annotation: B0034
sequence annotation: Start
sequence annotation: stem_loop
sequence annotation: lac promoter
sequence annotation: stop
sequence annotation: BBa_B0034
sequence annotation: BBa_B0010
sequence: https://synbiohub.org/public/igem/BBa_K1919500_sequence/1

BBa_K1321200
type: SequenceAnnotation
persistent identity: annotation2483241
display id: annotation2483241
version: 1
top level: BBa_K1919500
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1919500/annotation2483241/range2483241/1

https://synbiohub.org/public/igem/BBa_K1919500/annotation2483241/range2483241/1
type: Range
persistent identity: range2483241
display id: range2483241
version: 1
direction: direction/reverse
top level: BBa_K1919500
start: 1
end: 504
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2483255
display id: annotation2483255
version: 1
top level: BBa_K1919500
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1919500/annotation2483255/range2483255/1

https://synbiohub.org/public/igem/BBa_K1919500/annotation2483255/range2483255/1
type: Range
persistent identity: range2483255
display id: range2483255
version: 1
direction: direction/forward
top level: BBa_K1919500
start: 1541
end: 1581
orientation: inline

J23101 constitutive
type: SequenceAnnotation
persistent identity: annotation2483240
display id: annotation2483240
version: 1
top level: BBa_K1919500
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1919500/annotation2483240/range2483240/1

https://synbiohub.org/public/igem/BBa_K1919500/annotation2483240/range2483240/1
type: Range
persistent identity: range2483240
display id: range2483240
version: 1
direction: direction/forward
top level: BBa_K1919500
start: 1
end: 35
orientation: inline

Stop
type: SequenceAnnotation
persistent identity: annotation2483245
display id: annotation2483245
version: 1
top level: BBa_K1919500
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1919500/annotation2483245/range2483245/1

https://synbiohub.org/public/igem/BBa_K1919500/annotation2483245/range2483245/1
type: Range
persistent identity: range2483245
display id: range2483245
version: 1
direction: direction/forward
top level: BBa_K1919500
start: 502
end: 504
orientation: inline

CAP
type: SequenceAnnotation
persistent identity: annotation2483248
display id: annotation2483248
version: 1
top level: BBa_K1919500
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1919500/annotation2483248/range2483248/1

https://synbiohub.org/public/igem/BBa_K1919500/annotation2483248/range2483248/1
type: Range
persistent identity: range2483248
display id: range2483248
version: 1
direction: direction/forward
top level: BBa_K1919500
start: 601
end: 638
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation2483257
display id: annotation2483257
version: 1
top level: BBa_K1919500
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_K1919500/annotation2483257/range2483257/1

https://synbiohub.org/public/igem/BBa_K1919500/annotation2483257/range2483257/1
type: Range
persistent identity: range2483257
display id: range2483257
version: 1
direction: direction/forward
top level: BBa_K1919500
start: 1568
end: 1581
orientation: inline

BBa_E1010
type: SequenceAnnotation
persistent identity: annotation2483252
display id: annotation2483252
version: 1
top level: BBa_K1919500
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1919500/annotation2483252/range2483252/1

https://synbiohub.org/public/igem/BBa_K1919500/annotation2483252/range2483252/1
type: Range
persistent identity: range2483252
display id: range2483252
version: 1
direction: direction/forward
top level: BBa_K1919500
start: 739
end: 1419
orientation: inline

lac operator
type: SequenceAnnotation
persistent identity: annotation2483259
display id: annotation2483259
version: 1
top level: BBa_K1919500
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1919500/annotation2483259/range2483259/1

https://synbiohub.org/public/igem/BBa_K1919500/annotation2483259/range2483259/1
type: Range
persistent identity: range2483259
display id: range2483259
version: 1
direction: direction/forward
top level: BBa_K1919500
start: 687
end: 703
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation2483246
display id: annotation2483246
version: 1
top level: BBa_K1919500
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1919500/annotation2483246/range2483246/1

https://synbiohub.org/public/igem/BBa_K1919500/annotation2483246/range2483246/1
type: Range
persistent identity: range2483246
display id: range2483246
version: 1
direction: direction/forward
top level: BBa_K1919500
start: 513
end: 712
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation2483256
display id: annotation2483256
version: 1
top level: BBa_K1919500
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1919500/annotation2483256/range2483256/1

https://synbiohub.org/public/igem/BBa_K1919500/annotation2483256/range2483256/1
type: Range
persistent identity: range2483256
display id: range2483256
version: 1
direction: direction/forward
top level: BBa_K1919500
start: 1548
end: 1567
orientation: inline

mrfp1
type: SequenceAnnotation
persistent identity: annotation2483251
display id: annotation2483251
version: 1
top level: BBa_K1919500
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1919500/annotation2483251/range2483251/1

https://synbiohub.org/public/igem/BBa_K1919500/annotation2483251/range2483251/1
type: Range
persistent identity: range2483251
display id: range2483251
version: 1
direction: direction/forward
top level: BBa_K1919500
start: 739
end: 1413
orientation: inline

VHb
type: SequenceAnnotation
persistent identity: annotation2483244
display id: annotation2483244
version: 1
top level: BBa_K1919500
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1919500/annotation2483244/range2483244/1

https://synbiohub.org/public/igem/BBa_K1919500/annotation2483244/range2483244/1
type: Range
persistent identity: range2483244
display id: range2483244
version: 1
direction: direction/forward
top level: BBa_K1919500
start: 64
end: 504
orientation: inline

BBa_J04450
type: SequenceAnnotation
persistent identity: annotation2483247
display id: annotation2483247
version: 1
top level: BBa_K1919500
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1919500/annotation2483247/range2483247/1

https://synbiohub.org/public/igem/BBa_K1919500/annotation2483247/range2483247/1
type: Range
persistent identity: range2483247
display id: range2483247
version: 1
direction: direction/reverse
top level: BBa_K1919500
start: 513
end: 1581
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation2483242
display id: annotation2483242
version: 1
top level: BBa_K1919500
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1919500/annotation2483242/range2483242/1

https://synbiohub.org/public/igem/BBa_K1919500/annotation2483242/range2483242/1
type: Range
persistent identity: range2483242
display id: range2483242
version: 1
direction: direction/forward
top level: BBa_K1919500
start: 44
end: 55
orientation: inline

Start
type: SequenceAnnotation
persistent identity: annotation2483243
display id: annotation2483243
version: 1
top level: BBa_K1919500
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1919500/annotation2483243/range2483243/1

https://synbiohub.org/public/igem/BBa_K1919500/annotation2483243/range2483243/1
type: Range
persistent identity: range2483243
display id: range2483243
version: 1
direction: direction/forward
top level: BBa_K1919500
start: 64
end: 66
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation2483254
display id: annotation2483254
version: 1
top level: BBa_K1919500
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1919500/annotation2483254/range2483254/1

https://synbiohub.org/public/igem/BBa_K1919500/annotation2483254/range2483254/1
type: Range
persistent identity: range2483254
display id: range2483254
version: 1
direction: direction/forward
top level: BBa_K1919500
start: 1462
end: 1507
orientation: inline

lac promoter
type: SequenceAnnotation
persistent identity: annotation2483249
display id: annotation2483249
version: 1
top level: BBa_K1919500
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1919500/annotation2483249/range2483249/1

https://synbiohub.org/public/igem/BBa_K1919500/annotation2483249/range2483249/1
type: Range
persistent identity: range2483249
display id: range2483249
version: 1
direction: direction/forward
top level: BBa_K1919500
start: 649
end: 679
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2483258
display id: annotation2483258
version: 1
top level: BBa_K1919500
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1919500/annotation2483258/range2483258/1

https://synbiohub.org/public/igem/BBa_K1919500/annotation2483258/range2483258/1
type: Range
persistent identity: range2483258
display id: range2483258
version: 1
direction: direction/forward
top level: BBa_K1919500
start: 1575
end: 1575
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2483250
display id: annotation2483250
version: 1
top level: BBa_K1919500
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1919500/annotation2483250/range2483250/1

https://synbiohub.org/public/igem/BBa_K1919500/annotation2483250/range2483250/1
type: Range
persistent identity: range2483250
display id: range2483250
version: 1
direction: direction/forward
top level: BBa_K1919500
start: 721
end: 732
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2483253
display id: annotation2483253
version: 1
top level: BBa_K1919500
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1919500/annotation2483253/range2483253/1

https://synbiohub.org/public/igem/BBa_K1919500/annotation2483253/range2483253/1
type: Range
persistent identity: range2483253
display id: range2483253
version: 1
direction: direction/forward
top level: BBa_K1919500
start: 1453
end: 1532
orientation: inline

BBa_K1152003
type: ComponentDefinition
persistent identity: BBa K1152003
display id: BBa_K1152003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TycC6 Module from Tyrocidine
creator: Tania Christiansen
created: 2013-09-19T11:00:00Z
modified: 2015-05-08T01:09:29Z
discontinued: false
dominant: false
group u list: _1464_
m user id: 0
owner id: 16899
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Coding Sequence of TycC6
sequence annotation: ATG Start Codon
sequence: https://synbiohub.org/public/igem/BBa_K1152003_sequence/1

Coding Sequence of TycC6
type: SequenceAnnotation
persistent identity: annotation2367796
display id: annotation2367796
version: 1
top level: BBa_K1152003
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1152003/annotation2367796/range2367796/1

https://synbiohub.org/public/igem/BBa_K1152003/annotation2367796/range2367796/1
type: Range
persistent identity: range2367796
display id: range2367796
version: 1
direction: direction/forward
top level: BBa_K1152003
start: 5
end: 3876
orientation: inline

ATG Start Codon
type: SequenceAnnotation
persistent identity: annotation2367792
display id: annotation2367792
version: 1
top level: BBa_K1152003
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1152003/annotation2367792/range2367792/1

https://synbiohub.org/public/igem/BBa_K1152003/annotation2367792/range2367792/1
type: Range
persistent identity: range2367792
display id: range2367792
version: 1
direction: direction/forward
top level: BBa_K1152003
start: 5
end: 7
orientation: inline

BBa_K1342002
type: ComponentDefinition
persistent identity: BBa K1342002
display id: BBa_K1342002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: AspA LVA(-)
creator: Naoki Saito,Daiki Haraguchi,Yoshiharu Otaki,Ryuhei Minei
created: 2014-10-02T11:00:00Z
modified: 2015-05-08T01:09:59Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1717_
m user id: 0
owner id: 22434
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This is
mutable notes: soon
mutable provenance: it
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: cds
sequence: https://synbiohub.org/public/igem/BBa_K1342002_sequence/1

cds
type: SequenceAnnotation
persistent identity: annotation2394009
display id: annotation2394009
version: 1
top level: BBa_K1342002
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1342002/annotation2394009/range2394009/1

https://synbiohub.org/public/igem/BBa_K1342002/annotation2394009/range2394009/1
type: Range
persistent identity: range2394009
display id: range2394009
version: 1
direction: direction/forward
top level: BBa_K1342002
start: 1
end: 1482
orientation: inline

sam8
type: ComponentDefinition
persistent identity: BBa I742142
display id: BBa_I742142
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: sam8 (tyrosine-ammonia lyase) coding sequence
creator: sarah hollingshead
created: 2007-10-22T11:00:00Z
modified: 2015-08-31T04:08:03Z
discontinued: false
dominant: false
experience: experience/None
group u list: _123_
m user id: 0
owner id: 837
owning group id: 163
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This is the coding sequence of sam8, encoding tyrosine ammonia lyase, from the bacterium Saccharothrix espanaensis DSM 4429. The enzyme converts tyrosine to p-coumaric acid. Accession: ABC88666. Reference: Berner, M., Krug, D., Bihlmaier, C., Vente, A., Muller, R. and Bechthold, A. 'Genes and Enzymes Involved in Caffeic Acid Biosynthesis in the Actinomycete Saccharothrix espanaensis' J. Bacteriol. 188 (7), 2666-2673 (2006)
mutable notes: No important considerations.
mutable provenance: Saccharothrix espanaensis DSM 4429. Accession: ABC88666.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_I742142_sequence/1

pabA
type: ComponentDefinition
persistent identity: BBa K137005
display id: BBa_K137005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pabA (from pABA synthesis)
creator: Victoria Hsiao
created: 2008-06-19T11:00:00Z
modified: 2015-05-08T01:10:08Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _187_
m user id: 0
owner id: 2987
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: pabA is a gene involved in para-aminobenzoic acid (pABA) synthesis from chorismate. PABA is necessary for the synthesis of folate.
mutable notes: We decided to clone the pABA synthesis genes separately (even though they reside side by side) because L.lactis uses start codons (ttg) that E.coli cannot, and so we need to change the start codon for pabB to atg.
mutable provenance: L.lactis genome
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K137005_sequence/1

BBa_J45396
type: ComponentDefinition
persistent identity: BBa J45396
display id: BBa_J45396
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: BAT2 enzyme translational unit
creator: Andr?? Green II
created: 2006-10-27T11:00:00Z
modified: 2015-08-31T04:08:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _84_
m user id: 0
owner id: 977
owning group id: 84
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This part is an intermediate to the protein generator BBa_45400.
mutable notes: coming
mutable provenance: coming
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_B0030
component: BBa_J45008
sequence annotation: BBa_J45008
sequence annotation: BBa_B0030
sequence: https://synbiohub.org/public/igem/BBa_J45396_sequence/1

BBa_B0030
type: Component
persistent identity: component1907892
display id: component1907892
version: 1
top level: BBa_J45396
access: public
definition: https://synbiohub.org/public/igem/BBa_B0030/1

BBa_J45008
type: Component
persistent identity: component1907897
display id: component1907897
version: 1
top level: BBa_J45396
access: public
definition: https://synbiohub.org/public/igem/BBa_J45008/1

BBa_J45008
type: SequenceAnnotation
persistent identity: annotation1907897
display id: annotation1907897
version: 1
top level: BBa_J45396
location: https://synbiohub.org/public/igem/BBa_J45396/annotation1907897/range1907897/1
component: BBa_J45008

https://synbiohub.org/public/igem/BBa_J45396/annotation1907897/range1907897/1
type: Range
persistent identity: range1907897
display id: range1907897
version: 1
direction: direction/reverse
top level: BBa_J45396
start: 22
end: 1155
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation1907892
display id: annotation1907892
version: 1
top level: BBa_J45396
location: https://synbiohub.org/public/igem/BBa_J45396/annotation1907892/range1907892/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_J45396/annotation1907892/range1907892/1
type: Range
persistent identity: range1907892
display id: range1907892
version: 1
direction: direction/forward
top level: BBa_J45396
start: 1
end: 15
orientation: inline

BBa_K1686046
type: ComponentDefinition
persistent identity: BBa K1686046
display id: BBa_K1686046
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Curdlan synthase gene with codon optimisation for E. coli
creator: Jean Descarpentrie
created: 2015-09-05T11:00:00Z
modified: 2015-09-12T08:06:56Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2104_
m user id: 25369
owner id: 25369
owning group id: 9
status: status/Planning
bookmark: false
mutable description: The curdlan synthase (73 kDa) coded by crdS (1,965 bp) polymerizes UDP glucose into beta 1,3 D Glucan. CrdS shares no homology with the (1→3)- β-glucan synthase-related FSK1 and FSK2 proteins from fungi (e.g. Saccharomyces, Candida, Aspergillus; Dijkgraaf et al. 2001) or the plant callose synthase-related proteins (Li et al. 2003), which are both classified as GT48 glycosyltransferases. In Agrobacterium, CrdS is an integral inner membrane protein with seven transmembrane (TM) helices, one non-membrane-spanning amphipathic helix and a Nout???Cin disposition). In Agrobacterium, this gene is flancked by two other genes (crdA and crdC) who's corresponding proteins help transport the nascent polymer across the cytoplasmic membrane and into the periplasm. The process of polymerisation mediated by CrdS occurs on the cytoplasmic face of the inner membrane and so is unlikely to involve the periplasmic CrdC, or CrdA directly.
mutable notes: The gene sequence that was used was found in the publication written below. We then verified that the EcoRI, XbaI, SpeI, PstI sequences were not in the gene which was the case. Finally we optimized the sequence using IDT's optimization software on the internet for E.coli. GenBank : AF057142.2 Publication : ????Detection of two loci involved in (1→3)-β-glucan (curdlan) biosynthesis by Agrobacterium sp. ATCC31749, and comparative sequence analysis of the putative curdlan synthase gene???? , Stan J.Stasinopoulos & all, 1999
mutable provenance: Curdlan and other bacterial (1→3)-β-D-glucans. M. McIntosh . B. A. Stone . V. A. Stanisich - Appl Microbiol Biotechnol (2005) 68: 163???173; DOI 10.1007/s00253-005-1959-5 (Karnezis et al. 2000, 2003)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Stop codon
sequence annotation: Start codon
sequence: https://synbiohub.org/public/igem/BBa_K1686046_sequence/1

Stop codon
type: SequenceAnnotation
persistent identity: annotation2445184
display id: annotation2445184
version: 1
top level: BBa_K1686046
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1686046/annotation2445184/range2445184/1

https://synbiohub.org/public/igem/BBa_K1686046/annotation2445184/range2445184/1
type: Range
persistent identity: range2445184
display id: range2445184
version: 1
direction: direction/forward
top level: BBa_K1686046
start: 1963
end: 1965
orientation: inline

Start codon
type: SequenceAnnotation
persistent identity: annotation2445183
display id: annotation2445183
version: 1
top level: BBa_K1686046
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1686046/annotation2445183/range2445183/1

https://synbiohub.org/public/igem/BBa_K1686046/annotation2445183/range2445183/1
type: Range
persistent identity: range2445183
display id: range2445183
version: 1
direction: direction/forward
top level: BBa_K1686046
start: 1
end: 3
orientation: inline

BBa_I716340
type: ComponentDefinition
persistent identity: BBa I716340
display id: BBa_I716340
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: rbs-thpD-TT
creator: nhu nguyen
created: 2007-08-13T11:00:00Z
modified: 2015-08-31T04:07:52Z
discontinued: false
dominant: false
experience: experience/None
group u list: _110_
m user id: 0
owner id: 2095
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: rbs-ThpD-TT will produce hydoxyectoine to withstand freeze dry.
mutable notes: Digest I716338 (EcoRI/BamHI, 2057bp, 923bp, S) Sub into Sub into pBca9145-Bca1092 (EcoRI/BglII 2198bp, 9bp, L) Product is I716340
mutable provenance: {{BerkiGEM2007-Biobrick2.0Intro}}
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence: https://synbiohub.org/public/igem/BBa_I716340_sequence/1

BBa_I716338
type: ComponentDefinition
persistent identity: BBa I716338
display id: BBa_I716338
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: rbs-ThpD
creator: nhu nguyen
created: 2007-08-13T11:00:00Z
modified: 2015-08-31T04:07:52Z
discontinued: false
dominant: false
experience: experience/None
group u list: _110_
m user id: 0
owner id: 2095
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: rbs-thpD is the gene that will produce hydroxyectoine to withstand freeze dry.
mutable notes: Digest pBca9145-BBa I716334 (BglII/XhoI, 2063bp, 900bp, S) Sub into pJD507-pSB1A2C (BamHI/XhoI, 2071bp, 9bp, L) Product is I716338
mutable provenance: {{BerkiGEM2007-Biobrick2.0Intro}}
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
sequence: https://synbiohub.org/public/igem/BBa_I716338_sequence/1

BBa_K727001
type: ComponentDefinition
persistent identity: BBa K727001
display id: BBa_K727001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Monomethylamine Methyltransferase (MMT)
creator: Mustafa Elitok
created: 2012-06-15T11:00:00Z
modified: 2015-05-08T01:13:05Z
discontinued: false
dominant: false
experience: experience/None
group u list: _973_
m user id: 0
owner id: 9573
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: MMT (monomethylamine transferase) catalyses the first reaction of TMA (trimethylamine) synthesis. TMA is the major cause of unpleasant fish odor. For additional information: http://2012hs.igem.org/Team:AUC_Turkey/Project/TMA
mutable notes: Optimized for E.coli
mutable provenance: Genome of
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: MMT
sequence annotation: BBa_K727001
sequence: https://synbiohub.org/public/igem/BBa_K727001_sequence/1

MMT
type: SequenceAnnotation
persistent identity: annotation2176577
display id: annotation2176577
version: 1
top level: BBa_K727001
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K727001/annotation2176577/range2176577/1

https://synbiohub.org/public/igem/BBa_K727001/annotation2176577/range2176577/1
type: Range
persistent identity: range2176577
display id: range2176577
version: 1
direction: direction/forward
top level: BBa_K727001
start: 1
end: 1415
orientation: inline

BBa_K727001
type: SequenceAnnotation
persistent identity: annotation2176578
display id: annotation2176578
version: 1
top level: BBa_K727001
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K727001/annotation2176578/range2176578/1

https://synbiohub.org/public/igem/BBa_K727001/annotation2176578/range2176578/1
type: Range
persistent identity: range2176578
display id: range2176578
version: 1
direction: direction/reverse
top level: BBa_K727001
start: 1
end: 1415
orientation: inline

BBa_K108018
type: ComponentDefinition
persistent identity: BBa K108018
display id: BBa_K108018
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PhbAB
creator: Qi Liu
created: 2008-10-22T11:00:00Z
modified: 2015-05-08T01:09:04Z
discontinued: false
dominant: false
experience: experience/None
group u list: _222_
m user id: 0
owner id: 2671
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: coming...
mutable notes: PCR using primers with biobrick prefix and surfix, and then cloned into pSB1AC3.
mutable provenance: pBHR68
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: phbA
sequence annotation: phbB
sequence: https://synbiohub.org/public/igem/BBa_K108018_sequence/1

phbA
type: SequenceAnnotation
persistent identity: annotation1986972
display id: annotation1986972
version: 1
top level: BBa_K108018
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K108018/annotation1986972/range1986972/1

https://synbiohub.org/public/igem/BBa_K108018/annotation1986972/range1986972/1
type: Range
persistent identity: range1986972
display id: range1986972
version: 1
direction: direction/forward
top level: BBa_K108018
start: 1
end: 1179
orientation: inline

phbB
type: SequenceAnnotation
persistent identity: annotation1986973
display id: annotation1986973
version: 1
top level: BBa_K108018
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K108018/annotation1986973/range1986973/1

https://synbiohub.org/public/igem/BBa_K108018/annotation1986973/range1986973/1
type: Range
persistent identity: range1986973
display id: range1986973
version: 1
direction: direction/forward
top level: BBa_K108018
start: 1257
end: 1994
orientation: inline

BBa_K1072006
type: ComponentDefinition
persistent identity: BBa K1072006
display id: BBa_K1072006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Lux pR+RBS+α-ALS+2TM
creator: Ruichen, Yichong wang and Junjie zhang
created: 2013-09-06T11:00:00Z
modified: 2015-05-08T01:09:01Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1381_
m user id: 0
owner id: 16002
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: α-ALS gene placed under the control of Lux pR promoter. After co-transforming with <bbpart>BBa_K1072000 </bbpart> into E.coli cells,the luxR and AHL complex will activate the Lux pR promotor and transcript α-ALS and GFP genes.
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_R0062
component: BBa_B0010
component: BBa_K1072004
component: BBa_B0012
component: BBa_B0034
sequence annotation: BBa_B0034
sequence annotation: BBa_B0012
sequence annotation: BBa_R0062
sequence annotation: BBa_B0010
sequence annotation: BBa_K1072004
sequence: https://synbiohub.org/public/igem/BBa_K1072006_sequence/1

BBa_R0062
type: Component
persistent identity: component2352688
display id: component2352688
version: 1
top level: BBa_K1072006
access: public
definition: https://synbiohub.org/public/igem/BBa_R0062/1

BBa_B0010
type: Component
persistent identity: component2352697
display id: component2352697
version: 1
top level: BBa_K1072006
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_K1072004
type: Component
persistent identity: component2352696
display id: component2352696
version: 1
top level: BBa_K1072006
access: public
definition: https://synbiohub.org/public/igem/BBa_K1072004/1

BBa_B0012
type: Component
persistent identity: component2352699
display id: component2352699
version: 1
top level: BBa_K1072006
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0034
type: Component
persistent identity: component2352694
display id: component2352694
version: 1
top level: BBa_K1072006
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2352694
display id: annotation2352694
version: 1
top level: BBa_K1072006
location: https://synbiohub.org/public/igem/BBa_K1072006/annotation2352694/range2352694/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1072006/annotation2352694/range2352694/1
type: Range
persistent identity: range2352694
display id: range2352694
version: 1
direction: direction/reverse
top level: BBa_K1072006
start: 64
end: 75
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2352699
display id: annotation2352699
version: 1
top level: BBa_K1072006
location: https://synbiohub.org/public/igem/BBa_K1072006/annotation2352699/range2352699/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K1072006/annotation2352699/range2352699/1
type: Range
persistent identity: range2352699
display id: range2352699
version: 1
direction: direction/forward
top level: BBa_K1072006
start: 1843
end: 1883
orientation: inline

BBa_R0062
type: SequenceAnnotation
persistent identity: annotation2352688
display id: annotation2352688
version: 1
top level: BBa_K1072006
location: https://synbiohub.org/public/igem/BBa_K1072006/annotation2352688/range2352688/1
component: BBa_R0062

https://synbiohub.org/public/igem/BBa_K1072006/annotation2352688/range2352688/1
type: Range
persistent identity: range2352688
display id: range2352688
version: 1
direction: direction/forward
top level: BBa_K1072006
start: 1
end: 55
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2352697
display id: annotation2352697
version: 1
top level: BBa_K1072006
location: https://synbiohub.org/public/igem/BBa_K1072006/annotation2352697/range2352697/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K1072006/annotation2352697/range2352697/1
type: Range
persistent identity: range2352697
display id: range2352697
version: 1
direction: direction/forward
top level: BBa_K1072006
start: 1755
end: 1834
orientation: inline

BBa_K1072004
type: SequenceAnnotation
persistent identity: annotation2352696
display id: annotation2352696
version: 1
top level: BBa_K1072006
location: https://synbiohub.org/public/igem/BBa_K1072006/annotation2352696/range2352696/1
component: BBa_K1072004

https://synbiohub.org/public/igem/BBa_K1072006/annotation2352696/range2352696/1
type: Range
persistent identity: range2352696
display id: range2352696
version: 1
direction: direction/reverse
top level: BBa_K1072006
start: 82
end: 1746
orientation: inline

BBa_K108029
type: ComponentDefinition
persistent identity: BBa K108029
display id: BBa_K108029
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: XylS
creator: Qi Liu
created: 2008-10-22T11:00:00Z
modified: 2015-05-08T01:09:04Z
discontinued: false
dominant: false
experience: experience/None
group u list: _222_
m user id: 0
owner id: 2671
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: coming...
mutable notes: PCR using primers with biobrick prefix and surfix, and then cloned into pSB1AC3.
mutable provenance: pWW0
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: XylS
sequence: https://synbiohub.org/public/igem/BBa_K108029_sequence/1

XylS
type: SequenceAnnotation
persistent identity: annotation1986984
display id: annotation1986984
version: 1
top level: BBa_K108029
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K108029/annotation1986984/range1986984/1

https://synbiohub.org/public/igem/BBa_K108029/annotation1986984/range1986984/1
type: Range
persistent identity: range1986984
display id: range1986984
version: 1
direction: direction/forward
top level: BBa_K108029
start: 1
end: 963
orientation: inline

BBa_I716337
type: ComponentDefinition
persistent identity: BBa I716337
display id: BBa_I716337
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: rbs-ThpC
creator: nhu nguyen
created: 2007-08-13T11:00:00Z
modified: 2015-08-31T04:07:52Z
discontinued: false
dominant: false
experience: experience/None
group u list: _110_
m user id: 0
owner id: 2095
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: rbs-ThpC will help initiate the production of hydroxyectoine to withstand freeze dry.
mutable notes: Digest pBca9145-BBa I716333 (BglII/XhoI, 2063bp, 405bp, S) Sub into pJD507-pSB1A2C (BamHI/XhoI, 2071bp, 9bp, L) Product is I716337
mutable provenance: {{BerkiGEM2007-Biobrick2.0Intro}}
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
sequence: https://synbiohub.org/public/igem/BBa_I716337_sequence/1

BBa_K274200
type: ComponentDefinition
persistent identity: BBa K274200
display id: BBa_K274200
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CrtEBIY with rbs
creator: Siming Ma
created: 2009-09-17T11:00:00Z
modified: 2015-05-08T01:11:45Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _374_
m user id: 0
owner id: 5416
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This Composite Biobrick is created by standard assembly of Part BBa_K118014 (CrtE with rbs), Part BBa_K118006 (CrtB with rbs), Part BBa_K118005 (CrtI with rbs) and Part BBa_K118013 (CrtY with rbs), which are submitted by previous iGEM teams. The whole cassette is on plasmid pSB1A2 (high copy, Ampicillin resistance). Together, enzymes CrtE, CrtB, CrtI and CrtY convert colourless farnesyl pyrophosphate to orange beta-carotene (via intermediates geranylgeranyl pyroiphosphate, phytoene and lycopene). The orange beta-carotene pigment can then be used as a coloured signal output, e.g. for biosensors. There is already individual ribosome binding site before each enzyme gene sequence. Internal restriction sites have been removed by previous iGEM teams. Please refer to Parts BBa_K118014, BBa_K118006, BBa_K118005 and BBa_K118013 for more information on individual Biobricks components. This Composite Biobrick has been put under constitutive promoter R0011 (see Part BBa_K274210) and arabinose-inducible promoter I0500 (see Part BBa_274220), transformed and tested in E.coli strain MG1655. Amount of beta-carotene produced can be measured by photospectrometer with absorbance at 455nm (beta-carotene extraction using acetone). A related Composite Biobrick is Part BBa_K274100 (CrtEBI with rbs), which "stops" at one step before beta-carotene, producing (and retaining) red lycopene pigment.
mutable notes: The colourless precursor, farnesyl pyrophosphate, is naturally produced in E.coli using Non-mevalonate Pathway (from pyruvate and glyceraldehyde-3-phosphate). Our experiences showed that this Biobrick works better in E.coli strains with up-regulated level of precursors. E.coli strain MG1655 demonostrates higher level of pigment (beta-carotene) production than strain TOP10.
mutable provenance: Gene coding sequences come from Pantoea ananatis (formerly Erwinia uredovora) (Accession number D90087), a type of Gram negative Enterobacteriaceae. This Composite Biobrick is created by standard assembly of Part BBa_K118014, Part BBa_K118006, Part BBa_K118005 and Part BBa_K118013.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K118012
component: BBa_I742151
component: BBa_K118008
component: BBa_K118003
component: BBa_K118002
component: BBa_J15001
sequence annotation: BBa_K118012
sequence annotation: BBa_K118002
sequence annotation: BBa_J15001
sequence annotation: BBa_I742151
sequence annotation: BBa_K118003
sequence annotation: BBa_K118008
sequence: https://synbiohub.org/public/igem/BBa_K274200_sequence/1

BBa_K118012
type: Component
persistent identity: component2021580
display id: component2021580
version: 1
top level: BBa_K274200
access: public
definition: https://synbiohub.org/public/igem/BBa_K118012/1

BBa_I742151
type: Component
persistent identity: component2021581
display id: component2021581
version: 1
top level: BBa_K274200
access: public
definition: https://synbiohub.org/public/igem/BBa_I742151/1

BBa_K118008
type: Component
persistent identity: component2021596
display id: component2021596
version: 1
top level: BBa_K274200
access: public
definition: https://synbiohub.org/public/igem/BBa_K118008/1

BBa_K118003
type: Component
persistent identity: component2021593
display id: component2021593
version: 1
top level: BBa_K274200
access: public
definition: https://synbiohub.org/public/igem/BBa_K118003/1

BBa_K118002
type: Component
persistent identity: component2021586
display id: component2021586
version: 1
top level: BBa_K274200
access: public
definition: https://synbiohub.org/public/igem/BBa_K118002/1

BBa_K118012
type: Component
persistent identity: component2021594
display id: component2021594
version: 1
top level: BBa_K274200
access: public
definition: https://synbiohub.org/public/igem/BBa_K118012/1

BBa_J15001
type: Component
persistent identity: component2021584
display id: component2021584
version: 1
top level: BBa_K274200
access: public
definition: https://synbiohub.org/public/igem/BBa_J15001/1

BBa_J15001
type: Component
persistent identity: component2021589
display id: component2021589
version: 1
top level: BBa_K274200
access: public
definition: https://synbiohub.org/public/igem/BBa_J15001/1

BBa_K118012
type: SequenceAnnotation
persistent identity: annotation2021594
display id: annotation2021594
version: 1
top level: BBa_K274200
location: https://synbiohub.org/public/igem/BBa_K274200/annotation2021594/range2021594/1
component: BBa_K118012

https://synbiohub.org/public/igem/BBa_K274200/annotation2021594/range2021594/1
type: Range
persistent identity: range2021594
display id: range2021594
version: 1
direction: direction/reverse
top level: BBa_K274200
start: 3394
end: 3397
orientation: inline

BBa_K118002
type: SequenceAnnotation
persistent identity: annotation2021586
display id: annotation2021586
version: 1
top level: BBa_K274200
location: https://synbiohub.org/public/igem/BBa_K274200/annotation2021586/range2021586/1
component: BBa_K118002

https://synbiohub.org/public/igem/BBa_K274200/annotation2021586/range2021586/1
type: Range
persistent identity: range2021586
display id: range2021586
version: 1
direction: direction/reverse
top level: BBa_K274200
start: 947
end: 1879
orientation: inline

BBa_J15001
type: SequenceAnnotation
persistent identity: annotation2021589
display id: annotation2021589
version: 1
top level: BBa_K274200
location: https://synbiohub.org/public/igem/BBa_K274200/annotation2021589/range2021589/1
component: BBa_J15001

https://synbiohub.org/public/igem/BBa_K274200/annotation2021589/range2021589/1
type: Range
persistent identity: range2021589
display id: range2021589
version: 1
direction: direction/reverse
top level: BBa_K274200
start: 1888
end: 1897
orientation: inline

BBa_I742151
type: SequenceAnnotation
persistent identity: annotation2021581
display id: annotation2021581
version: 1
top level: BBa_K274200
location: https://synbiohub.org/public/igem/BBa_K274200/annotation2021581/range2021581/1
component: BBa_I742151

https://synbiohub.org/public/igem/BBa_K274200/annotation2021581/range2021581/1
type: Range
persistent identity: range2021581
display id: range2021581
version: 1
direction: direction/reverse
top level: BBa_K274200
start: 11
end: 922
orientation: inline

BBa_K118003
type: SequenceAnnotation
persistent identity: annotation2021593
display id: annotation2021593
version: 1
top level: BBa_K274200
location: https://synbiohub.org/public/igem/BBa_K274200/annotation2021593/range2021593/1
component: BBa_K118003

https://synbiohub.org/public/igem/BBa_K274200/annotation2021593/range2021593/1
type: Range
persistent identity: range2021593
display id: range2021593
version: 1
direction: direction/reverse
top level: BBa_K274200
start: 1904
end: 3385
orientation: inline

BBa_J15001
type: SequenceAnnotation
persistent identity: annotation2021584
display id: annotation2021584
version: 1
top level: BBa_K274200
location: https://synbiohub.org/public/igem/BBa_K274200/annotation2021584/range2021584/1
component: BBa_J15001

https://synbiohub.org/public/igem/BBa_K274200/annotation2021584/range2021584/1
type: Range
persistent identity: range2021584
display id: range2021584
version: 1
direction: direction/reverse
top level: BBa_K274200
start: 931
end: 940
orientation: inline

BBa_K118008
type: SequenceAnnotation
persistent identity: annotation2021596
display id: annotation2021596
version: 1
top level: BBa_K274200
location: https://synbiohub.org/public/igem/BBa_K274200/annotation2021596/range2021596/1
component: BBa_K118008

https://synbiohub.org/public/igem/BBa_K274200/annotation2021596/range2021596/1
type: Range
persistent identity: range2021596
display id: range2021596
version: 1
direction: direction/reverse
top level: BBa_K274200
start: 3404
end: 4555
orientation: inline

BBa_K118012
type: SequenceAnnotation
persistent identity: annotation2021580
display id: annotation2021580
version: 1
top level: BBa_K274200
location: https://synbiohub.org/public/igem/BBa_K274200/annotation2021580/range2021580/1
component: BBa_K118012

https://synbiohub.org/public/igem/BBa_K274200/annotation2021580/range2021580/1
type: Range
persistent identity: range2021580
display id: range2021580
version: 1
direction: direction/reverse
top level: BBa_K274200
start: 1
end: 4
orientation: inline

CqsA
type: ComponentDefinition
persistent identity: BBa K356000
display id: BBa_K356000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CAI-1 autoinducer synthase
creator: C David Owen, James Taylor
created: 2010-08-31T11:00:00Z
modified: 2015-05-08T01:12:11Z
discontinued: false
dominant: false
experience: experience/None
group u list: _467_
m user id: 0
owner id: 7465
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: CqsA gene from Vibrio cholerae, codes for the CAI-1 autoinducer synthase. This autoinducer is specific to V. cholerae and is detected by CqsS.
mutable notes: Codon optimized for expression in E. coli.
mutable provenance: Vibrio cholerae genomic sequence, from NCBI database, synthesized by GeneArt.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K356000_sequence/1

BBa_K130000
type: ComponentDefinition
persistent identity: BBa K130000
display id: BBa_K130000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: srlD
creator: Basudeb Bhattacharyya
created: 2008-10-27T12:00:00Z
modified: 2015-05-08T01:09:46Z
discontinued: false
dominant: false
experience: experience/None
group u list: _249_
m user id: 0
owner id: 2768
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: srlD ORF encoding for sorbitol dehydrogenase
mutable notes: Contains start and stop codon and consensus shine-dalgarno sequence.
mutable provenance: E. coli K12 (MG1655) genome.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
sequence annotation: Consensus RBS
sequence annotation: srlD ORF
sequence annotation: Start Codon
sequence: https://synbiohub.org/public/igem/BBa_K130000_sequence/1

Consensus RBS
type: SequenceAnnotation
persistent identity: annotation1992654
display id: annotation1992654
version: 1
top level: BBa_K130000
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K130000/annotation1992654/range1992654/1

https://synbiohub.org/public/igem/BBa_K130000/annotation1992654/range1992654/1
type: Range
persistent identity: range1992654
display id: range1992654
version: 1
direction: direction/forward
top level: BBa_K130000
start: 1
end: 6
orientation: inline

srlD ORF
type: SequenceAnnotation
persistent identity: annotation1992657
display id: annotation1992657
version: 1
top level: BBa_K130000
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K130000/annotation1992657/range1992657/1

https://synbiohub.org/public/igem/BBa_K130000/annotation1992657/range1992657/1
type: Range
persistent identity: range1992657
display id: range1992657
version: 1
direction: direction/forward
top level: BBa_K130000
start: 13
end: 792
orientation: inline

Start Codon
type: SequenceAnnotation
persistent identity: annotation1992656
display id: annotation1992656
version: 1
top level: BBa_K130000
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K130000/annotation1992656/range1992656/1

https://synbiohub.org/public/igem/BBa_K130000/annotation1992656/range1992656/1
type: Range
persistent identity: range1992656
display id: range1992656
version: 1
direction: direction/forward
top level: BBa_K130000
start: 13
end: 15
orientation: inline

BisdB
type: ComponentDefinition
persistent identity: BBa K123001
display id: BBa_K123001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: BisdB
creator: Jason Gardiner
created: 2008-10-27T12:00:00Z
modified: 2015-05-08T01:09:44Z
discontinued: false
dominant: false
experience: experience/None
group u list: _241_
m user id: 0
owner id: 2742
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: When used in conjunction with BisdA this enzyme can be used to degrade Bisphenol A. Activity has previously been recorded in E.coli by Miho Sasaki1 et al. 2005. Miho Sasaki1, Jun-ichi Maki, Ko-ichi Oshiman, Yoshinobu Matsumura1, and Tetsuaki Tsuchido.2005, Biodegradation of bisphenol A by cells and cell lysate from Sphingomonas sp. strain AO1. Biodegredation 16: 449-459
mutable notes: When used with BisdA the degredation of Bisphenol A sharply increases
mutable provenance: Sphingomonas sp. strain AO1
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K123001_sequence/1

frr
type: ComponentDefinition
persistent identity: BBa K1668002
display id: BBa_K1668002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: frr (from Streptomyces avermitilis, increasing avermectin production)
creator: Xintian Xu
created: 2015-09-07T11:00:00Z
modified: 2015-09-11T06:20:46Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2086_
m user id: 24510
owner id: 24510
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Frr gene encodes the ribosome recycling factor (RRF), which is involved in the release of ribosomes from the translational post-termination complex for a new round of initiation. In comparison to the wild-type strain, avermectin yield was increased significantly by 3- to 3.7-fold in transformants 31267(pFRR-1139) and 31267(pFRRerm-1139), both of which contained multiple frr copies, compared with that in the control strains. The avermectin productivity of each culture was quantitatively measured by HPLC analysis. Research indicated that frr overexpression promoted cell growth as well as the expression of ave genes (including pathway-specific positive regulatory gene aveR for avermectin biosynthesis and ave structural genes), leading in turn to avermectin overproduction. Different from S-adenosylmethionine synthetase gene (metK), frr gene revealed a ??????copy number effect??????: multiple copies of frr had a greater promoting effect on avermectin production than a single copy in each of the three transformed S. avermitilis strains.
mutable notes: We used seamless assembly.
mutable provenance: The metK gene was amplified by PCR with genomic DNA of S. avermitilis(具体型号) as template using primers
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: cds
sequence annotation: start coden
sequence annotation: stop coden
sequence: https://synbiohub.org/public/igem/BBa_K1668002_sequence/1

cds
type: SequenceAnnotation
persistent identity: annotation2456727
display id: annotation2456727
version: 1
top level: frr
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1668002/annotation2456727/range2456727/1

https://synbiohub.org/public/igem/BBa_K1668002/annotation2456727/range2456727/1
type: Range
persistent identity: range2456727
display id: range2456727
version: 1
direction: direction/forward
top level: frr
start: 1
end: 384
orientation: inline

start coden
type: SequenceAnnotation
persistent identity: annotation2449087
display id: annotation2449087
version: 1
top level: frr
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1668002/annotation2449087/range2449087/1

https://synbiohub.org/public/igem/BBa_K1668002/annotation2449087/range2449087/1
type: Range
persistent identity: range2449087
display id: range2449087
version: 1
direction: direction/forward
top level: frr
start: 1
end: 3
orientation: inline

stop coden
type: SequenceAnnotation
persistent identity: annotation2449088
display id: annotation2449088
version: 1
top level: frr
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1668002/annotation2449088/range2449088/1

https://synbiohub.org/public/igem/BBa_K1668002/annotation2449088/range2449088/1
type: Range
persistent identity: range2449088
display id: range2449088
version: 1
direction: direction/forward
top level: frr
start: 379
end: 384
orientation: inline

rbs+COMT
type: ComponentDefinition
persistent identity: BBa I742109
display id: BBa_I742109
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: COMT gene with ribosome binding site
creator: sarah hollingshead
created: 2007-10-18T11:00:00Z
modified: 2015-08-31T04:08:02Z
discontinued: false
dominant: false
experience: experience/None
group u list: _123_
m user id: 0
owner id: 1964
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: COMT (caffeic acid-O-methyl transferase) with ribosome binding site. Enzyme methylates -OH on C4 of the caffeic acid aromatic ring to produce ferulic acid. COMT is the third gene in the vanillin biosynthesis pathway,
mutable notes: Used COMT cDNA as genomic DNA contains introns
mutable provenance: Genes and Enzymes Involved in Caffeic Acid Biosynthesis in the Actinomycete Saccharothrix esanaensis, Berner M, et al, Journal of Bacteriology 188(7): 2666, (2006) Gowri G, et al, Molecular cloning and expression of alfalfa S-adenosyl-L-methionine: caffeic acid 3-0-methyltransferase, a key enzyme of lignin biosynthesis, Plant Physiology 97(1):7 (1991) Downregulation of Caffeic Acid 3-O-Methyltransferase and Caffeoyl CoA 3-O-Methyltransferase in Transgenic Alfalfa Impacts on Lignin Structure and Implications for the Biosynthesis of G and S Lignin, Guo D, et al, Plant Cell. January; 13(1): 73 (2001)
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_I742107
component: BBa_J15001
sequence annotation: BBa_J15001
sequence annotation: BBa_I742107
sequence: https://synbiohub.org/public/igem/BBa_I742109_sequence/1

BBa_I742107
type: Component
persistent identity: component1949771
display id: component1949771
version: 1
top level: rbs+COMT
access: public
definition: https://synbiohub.org/public/igem/BBa_I742107/1

BBa_J15001
type: Component
persistent identity: component1949770
display id: component1949770
version: 1
top level: rbs+COMT
access: public
definition: https://synbiohub.org/public/igem/BBa_J15001/1

BBa_J15001
type: SequenceAnnotation
persistent identity: annotation1949770
display id: annotation1949770
version: 1
top level: rbs+COMT
location: https://synbiohub.org/public/igem/BBa_I742109/annotation1949770/range1949770/1
component: BBa_J15001

https://synbiohub.org/public/igem/BBa_I742109/annotation1949770/range1949770/1
type: Range
persistent identity: range1949770
display id: range1949770
version: 1
direction: direction/reverse
top level: rbs+COMT
start: 1
end: 10
orientation: inline

BBa_I742107
type: SequenceAnnotation
persistent identity: annotation1949771
display id: annotation1949771
version: 1
top level: rbs+COMT
location: https://synbiohub.org/public/igem/BBa_I742109/annotation1949771/range1949771/1
component: BBa_I742107

https://synbiohub.org/public/igem/BBa_I742109/annotation1949771/range1949771/1
type: Range
persistent identity: range1949771
display id: range1949771
version: 1
direction: direction/reverse
top level: rbs+COMT
start: 17
end: 1117
orientation: inline

BBa_K284999
type: ComponentDefinition
persistent identity: BBa K284999
display id: BBa_K284999
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Deletar este tamb??m
creator: Johana Rincones
created: 2009-05-15T11:00:00Z
modified: 2015-05-08T01:11:48Z
discontinued: false
dominant: false
group u list: _386_
m user id: 0
owner id: 4137
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This version of the LuxC comes from Vibrio salmonicida. Team Example used it to build a bacterial lava lamp.
mutable notes: No modifications were necessary in order to comply with the standard.
mutable provenance: Vibrio salmonicida hypothetical protein EJN1 gene, complete cds; luminescence gene cluster, complete sequence; and RibG (ribG) gene, complete cds. NCBI Accession number is AF452135 REGION: 2346..3776
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Vs_LuxC protein coding sequence
sequence annotation: TAA Stop codon
sequence annotation: ATG start codon
sequence: https://synbiohub.org/public/igem/BBa_K284999_sequence/1

Vs_LuxC protein coding sequence
type: SequenceAnnotation
persistent identity: annotation2003117
display id: annotation2003117
version: 1
top level: BBa_K284999
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K284999/annotation2003117/range2003117/1

https://synbiohub.org/public/igem/BBa_K284999/annotation2003117/range2003117/1
type: Range
persistent identity: range2003117
display id: range2003117
version: 1
direction: direction/forward
top level: BBa_K284999
start: 1
end: 1431
orientation: inline

TAA Stop codon
type: SequenceAnnotation
persistent identity: annotation2003104
display id: annotation2003104
version: 1
top level: BBa_K284999
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K284999/annotation2003104/range2003104/1

https://synbiohub.org/public/igem/BBa_K284999/annotation2003104/range2003104/1
type: Range
persistent identity: range2003104
display id: range2003104
version: 1
direction: direction/forward
top level: BBa_K284999
start: 1429
end: 1431
orientation: inline

ATG start codon
type: SequenceAnnotation
persistent identity: annotation2003098
display id: annotation2003098
version: 1
top level: BBa_K284999
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K284999/annotation2003098/range2003098/1

https://synbiohub.org/public/igem/BBa_K284999/annotation2003098/range2003098/1
type: Range
persistent identity: range2003098
display id: range2003098
version: 1
direction: direction/forward
top level: BBa_K284999
start: 1
end: 3
orientation: inline

rbs+sam5
type: ComponentDefinition
persistent identity: BBa I742147
display id: BBa_I742147
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: sam5 (coumarate hydroxylase) with native rbs.
creator: sarah hollingshead
created: 2007-10-23T11:00:00Z
modified: 2015-08-31T04:08:03Z
discontinued: false
dominant: false
experience: experience/None
group u list: _123_
m user id: 0
owner id: 837
owning group id: 163
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: Sam5 (4-coumarate-3-hydroxylase) of the bacterium Saccharothrix espanaensis DSM 44229 with its native rbs.
mutable notes: This was made as a single biobrick, rather than separate rbs and coding sequence, hence the use of blunt ligation in generating this composite part.
mutable provenance: Saccharothrix espanaensis DSM 44229
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_I742145
component: BBa_I742141
sequence annotation: BBa_I742145
sequence annotation: BBa_I742141
sequence: https://synbiohub.org/public/igem/BBa_I742147_sequence/1

BBa_I742145
type: Component
persistent identity: component1953792
display id: component1953792
version: 1
top level: rbs+sam5
access: public
definition: https://synbiohub.org/public/igem/BBa_I742145/1

BBa_I742141
type: Component
persistent identity: component1953794
display id: component1953794
version: 1
top level: rbs+sam5
access: public
definition: sam5

BBa_I742145
type: SequenceAnnotation
persistent identity: annotation1953792
display id: annotation1953792
version: 1
top level: rbs+sam5
location: https://synbiohub.org/public/igem/BBa_I742147/annotation1953792/range1953792/1
component: BBa_I742145

https://synbiohub.org/public/igem/BBa_I742147/annotation1953792/range1953792/1
type: Range
persistent identity: range1953792
display id: range1953792
version: 1
direction: direction/reverse
top level: rbs+sam5
start: 1
end: 16
orientation: inline

BBa_I742141
type: SequenceAnnotation
persistent identity: annotation1953794
display id: annotation1953794
version: 1
top level: rbs+sam5
location: https://synbiohub.org/public/igem/BBa_I742147/annotation1953794/range1953794/1
component: BBa_I742141

https://synbiohub.org/public/igem/BBa_I742147/annotation1953794/range1953794/1
type: Range
persistent identity: range1953794
display id: range1953794
version: 1
direction: direction/reverse
top level: rbs+sam5
start: 17
end: 1558
orientation: inline

BBa_K1685011
type: ComponentDefinition
persistent identity: BBa K1685011
display id: BBa_K1685011
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Rhamnolipid production
creator: ITB_Indonesia 2015
created: 2015-09-15T11:00:00Z
modified: 2015-09-18T09:45:52Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2103_
m user id: 25100
owner id: 25100
owning group id: 9
status: status/Planning
bookmark: true
mutable description: This part encodes two enzymes, rhlA and rhlB (BBa_K653000), for biosynthesis of rhamnolipid. The enzymes are under T7 promoter with medium strengh and a strong ribosomal binding site (BBa_K613010), and stopped with a double terminator (BBa_B0015). The promoter had the second highest efficiency as shown by EPFL team 2011 (http://parts.igem.org/Part:BBa_K613010:Experience).
mutable notes: We include the scar sequences between RBS-coding and coding-terminator in hope that it will function properly.
mutable provenance: The part was chemically synthesised. The sequences were based on parts available in Registry.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K613010
component: BBa_K653000
component: BBa_B0015
sequence annotation: BBa_B0015
sequence annotation: BBa_K653000
sequence annotation: BBa_K613010
sequence: https://synbiohub.org/public/igem/BBa_K1685011_sequence/1

BBa_K613010
type: Component
persistent identity: component2461648
display id: component2461648
version: 1
top level: BBa_K1685011
access: public
definition: https://synbiohub.org/public/igem/BBa_K613010/1

BBa_K653000
type: Component
persistent identity: component2461658
display id: component2461658
version: 1
top level: BBa_K1685011
access: public
definition: Biosurfact

BBa_B0015
type: Component
persistent identity: component2461665
display id: component2461665
version: 1
top level: BBa_K1685011
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2461665
display id: annotation2461665
version: 1
top level: BBa_K1685011
location: https://synbiohub.org/public/igem/BBa_K1685011/annotation2461665/range2461665/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K1685011/annotation2461665/range2461665/1
type: Range
persistent identity: range2461665
display id: range2461665
version: 1
direction: direction/reverse
top level: BBa_K1685011
start: 2445
end: 2573
orientation: inline

BBa_K653000
type: SequenceAnnotation
persistent identity: annotation2461658
display id: annotation2461658
version: 1
top level: BBa_K1685011
location: https://synbiohub.org/public/igem/BBa_K1685011/annotation2461658/range2461658/1
component: BBa_K653000

https://synbiohub.org/public/igem/BBa_K1685011/annotation2461658/range2461658/1
type: Range
persistent identity: range2461658
display id: range2461658
version: 1
direction: direction/reverse
top level: BBa_K1685011
start: 123
end: 2436
orientation: inline

BBa_K613010
type: SequenceAnnotation
persistent identity: annotation2461648
display id: annotation2461648
version: 1
top level: BBa_K1685011
location: https://synbiohub.org/public/igem/BBa_K1685011/annotation2461648/range2461648/1
component: BBa_K613010

https://synbiohub.org/public/igem/BBa_K1685011/annotation2461648/range2461648/1
type: Range
persistent identity: range2461648
display id: range2461648
version: 1
direction: direction/reverse
top level: BBa_K1685011
start: 1
end: 114
orientation: inline

BBa_K081008
type: ComponentDefinition
persistent identity: BBa K081008
display id: BBa_K081008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: luxI protein generator (TERM-)
creator: Lorenzo Pasotti, Paolo Magni
created: 2008-10-17T11:00:00Z
modified: 2015-05-08T01:08:35Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _227_
m user id: 0
owner id: 2583
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: luxI protein synthesizes 3OC6HSL, which binds to LuxR. Strong RBS (efficiency=0.6).
mutable notes: We used BioBrick Standard Assembly.
mutable provenance: luxI: RBS:
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_C0061
component: BBa_B0030
sequence annotation: BBa_C0061
sequence annotation: BBa_B0030
sequence: https://synbiohub.org/public/igem/BBa_K081008_sequence/1

BBa_C0061
type: Component
persistent identity: component2243991
display id: component2243991
version: 1
top level: BBa_K081008
access: public
definition: https://synbiohub.org/public/igem/BBa_C0061/1

BBa_B0030
type: Component
persistent identity: component2243988
display id: component2243988
version: 1
top level: BBa_K081008
access: public
definition: https://synbiohub.org/public/igem/BBa_B0030/1

BBa_C0061
type: SequenceAnnotation
persistent identity: annotation2243991
display id: annotation2243991
version: 1
top level: BBa_K081008
location: https://synbiohub.org/public/igem/BBa_K081008/annotation2243991/range2243991/1
component: BBa_C0061

https://synbiohub.org/public/igem/BBa_K081008/annotation2243991/range2243991/1
type: Range
persistent identity: range2243991
display id: range2243991
version: 1
direction: direction/forward
top level: BBa_K081008
start: 22
end: 639
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation2243988
display id: annotation2243988
version: 1
top level: BBa_K081008
location: https://synbiohub.org/public/igem/BBa_K081008/annotation2243988/range2243988/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_K081008/annotation2243988/range2243988/1
type: Range
persistent identity: range2243988
display id: range2243988
version: 1
direction: direction/forward
top level: BBa_K081008
start: 1
end: 15
orientation: inline

BBa_I716336
type: ComponentDefinition
persistent identity: BBa I716336
display id: BBa_I716336
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: rbs-ThpB
creator: nhu nguyen
created: 2007-08-13T11:00:00Z
modified: 2015-08-31T04:07:52Z
discontinued: false
dominant: false
experience: experience/None
group u list: _110_
m user id: 0
owner id: 2095
owning group id: 9
sample status: No part sequence
status: status/Unavailable
bookmark: true
mutable description: Similarly to OtsA and OtsB, rbs-ThpB will help initiate the production of hydroxyectoine to withstand freeze dry.
mutable notes: Digest I716332 (BglII/XhoI, 2063bp, 1269bp, S) Sub into pJD507-pSB1A2C (BamHI/XhoI, 2071bp, 9bp, L) Product is I716336
mutable provenance: {{BerkiGEM2007-Biobrick2.0Intro}}
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804

BBa_K727571
type: ComponentDefinition
persistent identity: BBa K727571
display id: BBa_K727571
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Rose Odor Generator Device (Geraniol)
creator: İBRAHİM YASİR ORHAN
created: 2012-06-15T11:00:00Z
modified: 2015-05-08T01:13:05Z
discontinued: true
dominant: false
experience: experience/None
group u list: _973_
m user id: 0
owner id: 11153
owning group id: 9
sample status: Discontinued
status: status/Deleted
bookmark: false
mutable description: This part is assembled with Geranyl Diphosphate Synthase (http://partsregistry.org/wiki/index.php?title=Part:BBa_K727005) and Geraniol synthase (BBa_K727006)in order to produce geraniol. Geraniol is a monoterpenoid and an alcohol. It???s molecular formula is C10H18O . It is the primary part of rose oil, palmarosa oil, and citronella oil (Java type). It also occurs in small quantities in geranium, lemon, and many other essential oils. It has a rose-like odor and is commonly used in perfumes. It is used in flavors such as peach, raspberry, grapefruit, red apple, plum, lime, orange, lemon, watermelon, pineapple, and blueberry.
mutable notes: Optimized for E.coli
mutable provenance: Genome of
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_R0063
component: BBa_K727006
component: BBa_K727005
sequence annotation: BBa_R0063
sequence annotation: BBa_K727005
sequence annotation: BBa_K727006
sequence: https://synbiohub.org/public/igem/BBa_K727571_sequence/1

BBa_R0063
type: Component
persistent identity: component2176282
display id: component2176282
version: 1
top level: BBa_K727571
access: public
definition: https://synbiohub.org/public/igem/BBa_R0063/1

BBa_K727006
type: Component
persistent identity: component2176290
display id: component2176290
version: 1
top level: BBa_K727571
access: public
definition: https://synbiohub.org/public/igem/BBa_K727006/1

BBa_K727005
type: Component
persistent identity: component2176288
display id: component2176288
version: 1
top level: BBa_K727571
access: public
definition: https://synbiohub.org/public/igem/BBa_K727005/1

BBa_R0063
type: SequenceAnnotation
persistent identity: annotation2176282
display id: annotation2176282
version: 1
top level: BBa_K727571
location: https://synbiohub.org/public/igem/BBa_K727571/annotation2176282/range2176282/1
component: BBa_R0063

https://synbiohub.org/public/igem/BBa_K727571/annotation2176282/range2176282/1
type: Range
persistent identity: range2176282
display id: range2176282
version: 1
direction: direction/forward
top level: BBa_K727571
start: 1
end: 151
orientation: inline

BBa_K727005
type: SequenceAnnotation
persistent identity: annotation2176288
display id: annotation2176288
version: 1
top level: BBa_K727571
location: https://synbiohub.org/public/igem/BBa_K727571/annotation2176288/range2176288/1
component: BBa_K727005

https://synbiohub.org/public/igem/BBa_K727571/annotation2176288/range2176288/1
type: Range
persistent identity: range2176288
display id: range2176288
version: 1
direction: direction/reverse
top level: BBa_K727571
start: 160
end: 1100
orientation: inline

BBa_K727006
type: SequenceAnnotation
persistent identity: annotation2176290
display id: annotation2176290
version: 1
top level: BBa_K727571
location: https://synbiohub.org/public/igem/BBa_K727571/annotation2176290/range2176290/1
component: BBa_K727006

https://synbiohub.org/public/igem/BBa_K727571/annotation2176290/range2176290/1
type: Range
persistent identity: range2176290
display id: range2176290
version: 1
direction: direction/reverse
top level: BBa_K727571
start: 1109
end: 2958
orientation: inline

sam5
type: ComponentDefinition
persistent identity: BBa I742141
display id: BBa_I742141
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: sam5 (coumarate hydroxylase) coding sequence
creator: sarah hollingshead
created: 2007-10-22T11:00:00Z
modified: 2015-08-31T04:08:03Z
discontinued: false
dominant: false
experience: experience/None
group u list: _123_
m user id: 0
owner id: 837
owning group id: 163
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This is the coding sequence of sam5, encoding 4-coumarate-3-hydroxylase from the bacterium Saccharothrix espanaensis. The enzyme converts p-coumaric acid to caffeic acid. Accession: ABC88666. Reference: Berner, M., Krug, D., Bihlmaier, C., Vente, A., Muller, R. and Bechthold, A. 'Genes and Enzymes Involved in Caffeic Acid Biosynthesis in the Actinomycete Saccharothrix espanaensis' J. Bacteriol. 188 (7), 2666-2673 (2006). Note: contains a PstI site. This will be removed by site directed mutagenesis in due course but is still present, near the 3' end, in this version.
mutable notes: Note that this version of the gene contains a PstI site near the 5' end. This will ultimately be removed by site directed mutagenesis.
mutable provenance: Saccharothrix espanaensis DSM 44229, sequence accession ABC88666.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: PstI site to be removed
sequence: https://synbiohub.org/public/igem/BBa_I742141_sequence/1

PstI site to be removed
type: SequenceAnnotation
persistent identity: annotation1952768
display id: annotation1952768
version: 1
top level: sam5
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I742141/annotation1952768/range1952768/1

https://synbiohub.org/public/igem/BBa_I742141/annotation1952768/range1952768/1
type: Range
persistent identity: range1952768
display id: range1952768
version: 1
direction: direction/forward
top level: sam5
start: 1420
end: 1425
orientation: inline

BBa_K417007
type: ComponentDefinition
persistent identity: BBa K417007
display id: BBa_K417007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Simple inducible (LacI) ethylene generator
creator: Ben Porebski, Andrew Perry
created: 2010-10-25T11:00:00Z
modified: 2015-05-08T01:12:28Z
discontinued: false
dominant: false
experience: experience/None
group u list: _537_
m user id: 0
owner id: 6167
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: A simpler version of BBa_K417006 which expresses ACC synthase and ACC oxidase (EFE) to produce ethylene. This version does not express the SAM synthetase enzyme (BBa_K417000) and relies on S-adenosyl methionine generated by the chassis.
mutable notes: Native LacI promoter (BBa_R0010) was chosen since it facilitates easier assembly compared with the shorter alternative BBa_R0011, although it would be worth testing both these promoters. It is unknown if the order of the coding sequences is important in this device.
mutable provenance: See part documentation for enzyme coding sequences BBa_K417001 and BBa_K417002 for details on the origin of the sequences.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_B0034
component: BBa_K417002
component: BBa_B0012
component: BBa_B0010
component: BBa_K417001
component: BBa_R0010
sequence annotation: BBa_B0034
sequence annotation: BBa_B0012
sequence annotation: BBa_K417001
sequence annotation: BBa_R0010
sequence annotation: BBa_K417002
sequence annotation: BBa_B0010
sequence: https://synbiohub.org/public/igem/BBa_K417007_sequence/1

BBa_B0034
type: Component
persistent identity: component2103056
display id: component2103056
version: 1
top level: BBa_K417007
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K417002
type: Component
persistent identity: component2103060
display id: component2103060
version: 1
top level: BBa_K417007
access: public
definition: ACCoxidase

BBa_B0012
type: Component
persistent identity: component2103063
display id: component2103063
version: 1
top level: BBa_K417007
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0034
type: Component
persistent identity: component2103050
display id: component2103050
version: 1
top level: BBa_K417007
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0010
type: Component
persistent identity: component2103061
display id: component2103061
version: 1
top level: BBa_K417007
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_K417001
type: Component
persistent identity: component2103054
display id: component2103054
version: 1
top level: BBa_K417007
access: public
definition: ACCsynth

BBa_R0010
type: Component
persistent identity: component2103042
display id: component2103042
version: 1
top level: BBa_K417007
access: public
definition: https://synbiohub.org/public/igem/BBa_R0010/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2103050
display id: annotation2103050
version: 1
top level: BBa_K417007
location: https://synbiohub.org/public/igem/BBa_K417007/annotation2103050/range2103050/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K417007/annotation2103050/range2103050/1
type: Range
persistent identity: range2103050
display id: range2103050
version: 1
direction: direction/reverse
top level: BBa_K417007
start: 209
end: 220
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2103063
display id: annotation2103063
version: 1
top level: BBa_K417007
location: https://synbiohub.org/public/igem/BBa_K417007/annotation2103063/range2103063/1
component: BBa_B0012

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type: Range
persistent identity: range2103063
display id: range2103063
version: 1
direction: direction/forward
top level: BBa_K417007
start: 2587
end: 2627
orientation: inline

BBa_K417001
type: SequenceAnnotation
persistent identity: annotation2103054
display id: annotation2103054
version: 1
top level: BBa_K417007
location: https://synbiohub.org/public/igem/BBa_K417007/annotation2103054/range2103054/1
component: BBa_K417001

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type: Range
persistent identity: range2103054
display id: range2103054
version: 1
direction: direction/reverse
top level: BBa_K417007
start: 227
end: 1516
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation2103042
display id: annotation2103042
version: 1
top level: BBa_K417007
location: https://synbiohub.org/public/igem/BBa_K417007/annotation2103042/range2103042/1
component: BBa_R0010

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type: Range
persistent identity: range2103042
display id: range2103042
version: 1
direction: direction/forward
top level: BBa_K417007
start: 1
end: 200
orientation: inline

BBa_K417002
type: SequenceAnnotation
persistent identity: annotation2103060
display id: annotation2103060
version: 1
top level: BBa_K417007
location: https://synbiohub.org/public/igem/BBa_K417007/annotation2103060/range2103060/1
component: BBa_K417002

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type: Range
persistent identity: range2103060
display id: range2103060
version: 1
direction: direction/reverse
top level: BBa_K417007
start: 1543
end: 2490
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2103056
display id: annotation2103056
version: 1
top level: BBa_K417007
location: https://synbiohub.org/public/igem/BBa_K417007/annotation2103056/range2103056/1
component: BBa_B0034

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type: Range
persistent identity: range2103056
display id: range2103056
version: 1
direction: direction/reverse
top level: BBa_K417007
start: 1525
end: 1536
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2103061
display id: annotation2103061
version: 1
top level: BBa_K417007
location: https://synbiohub.org/public/igem/BBa_K417007/annotation2103061/range2103061/1
component: BBa_B0010

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type: Range
persistent identity: range2103061
display id: range2103061
version: 1
direction: direction/forward
top level: BBa_K417007
start: 2499
end: 2578
orientation: inline

BBa_K1067002
type: ComponentDefinition
persistent identity: BBa K1067002
display id: BBa_K1067002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Cytochrome A and X from Nitrosomonas europaea
creator: Kristian Davidsen and Helen Cook
created: 2013-09-18T11:00:00Z
modified: 2015-05-08T01:09:00Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1376_
m user id: 0
owner id: 16028
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: ggg
mutable notes: ggfd
mutable provenance: ggf
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: start
sequence annotation: stop
sequence annotation: protein
sequence annotation: Signal peptide
sequence annotation: cycA
sequence: https://synbiohub.org/public/igem/BBa_K1067002_sequence/1

start
type: SequenceAnnotation
persistent identity: annotation2359450
display id: annotation2359450
version: 1
top level: BBa_K1067002
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1067002/annotation2359450/range2359450/1

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type: Range
persistent identity: range2359450
display id: range2359450
version: 1
direction: direction/forward
top level: BBa_K1067002
start: 20
end: 22
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2359453
display id: annotation2359453
version: 1
top level: BBa_K1067002
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1067002/annotation2359453/range2359453/1

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type: Range
persistent identity: range2359453
display id: range2359453
version: 1
direction: direction/forward
top level: BBa_K1067002
start: 725
end: 727
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2359454
display id: annotation2359454
version: 1
top level: BBa_K1067002
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1067002/annotation2359454/range2359454/1

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type: Range
persistent identity: range2359454
display id: range2359454
version: 1
direction: direction/forward
top level: BBa_K1067002
start: 1429
end: 1431
orientation: inline

protein
type: SequenceAnnotation
persistent identity: annotation2359456
display id: annotation2359456
version: 1
top level: BBa_K1067002
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1067002/annotation2359456/range2359456/1

https://synbiohub.org/public/igem/BBa_K1067002/annotation2359456/range2359456/1
type: Range
persistent identity: range2359456
display id: range2359456
version: 1
direction: direction/forward
top level: BBa_K1067002
start: 730
end: 1431
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2359455
display id: annotation2359455
version: 1
top level: BBa_K1067002
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1067002/annotation2359455/range2359455/1

https://synbiohub.org/public/igem/BBa_K1067002/annotation2359455/range2359455/1
type: Range
persistent identity: range2359455
display id: range2359455
version: 1
direction: direction/forward
top level: BBa_K1067002
start: 730
end: 732
orientation: inline

Signal peptide
type: SequenceAnnotation
persistent identity: annotation2359449
display id: annotation2359449
version: 1
top level: BBa_K1067002
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1067002/annotation2359449/range2359449/1

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type: Range
persistent identity: range2359449
display id: range2359449
version: 1
direction: direction/forward
top level: BBa_K1067002
start: 20
end: 91
orientation: inline

cycA
type: SequenceAnnotation
persistent identity: annotation2359452
display id: annotation2359452
version: 1
top level: BBa_K1067002
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1067002/annotation2359452/range2359452/1

https://synbiohub.org/public/igem/BBa_K1067002/annotation2359452/range2359452/1
type: Range
persistent identity: range2359452
display id: range2359452
version: 1
direction: direction/forward
top level: BBa_K1067002
start: 92
end: 727
orientation: inline

BBa_K1067006
type: ComponentDefinition
persistent identity: BBa K1067006
display id: BBa_K1067006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Denitrification operon from Pseudomonas aeruginosa
creator: Kristian Davidsen, Helen Cook and Jakob Berg Jespersen
created: 2013-09-23T11:00:00Z
modified: 2015-05-08T01:09:00Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1376_
m user id: 0
owner id: 16028
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: fff
mutable notes: fff
mutable provenance: fff
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: cytochrome c-551
sequence annotation: Heme d1 biosynthesis protein
sequence annotation: Uroporphyrin-III c-methyltransferase
sequence annotation: Nitrite reductase
sequence annotation: cytochrome c precursor
sequence annotation: c-type cytochrome
sequence: https://synbiohub.org/public/igem/BBa_K1067006_sequence/1

cytochrome c-551
type: SequenceAnnotation
persistent identity: annotation2366467
display id: annotation2366467
version: 1
top level: BBa_K1067006
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1067006/annotation2366467/range2366467/1

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type: Range
persistent identity: range2366467
display id: range2366467
version: 1
direction: direction/forward
top level: BBa_K1067006
start: 1758
end: 2072
orientation: inline

Heme d1 biosynthesis protein
type: SequenceAnnotation
persistent identity: annotation2366475
display id: annotation2366475
version: 1
top level: BBa_K1067006
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1067006/annotation2366475/range2366475/1

https://synbiohub.org/public/igem/BBa_K1067006/annotation2366475/range2366475/1
type: Range
persistent identity: range2366475
display id: range2366475
version: 1
direction: direction/forward
top level: BBa_K1067006
start: 4061
end: 4585
orientation: inline

Uroporphyrin-III c-methyltransferase
type: SequenceAnnotation
persistent identity: annotation2366477
display id: annotation2366477
version: 1
top level: BBa_K1067006
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1067006/annotation2366477/range2366477/1

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type: Range
persistent identity: range2366477
display id: range2366477
version: 1
direction: direction/forward
top level: BBa_K1067006
start: 6679
end: 7518
orientation: inline

Nitrite reductase
type: SequenceAnnotation
persistent identity: annotation2366465
display id: annotation2366465
version: 1
top level: BBa_K1067006
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1067006/annotation2366465/range2366465/1

https://synbiohub.org/public/igem/BBa_K1067006/annotation2366465/range2366465/1
type: Range
persistent identity: range2366465
display id: range2366465
version: 1
direction: direction/forward
top level: BBa_K1067006
start: 1
end: 1707
orientation: inline

cytochrome c precursor
type: SequenceAnnotation
persistent identity: annotation2366470
display id: annotation2366470
version: 1
top level: BBa_K1067006
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1067006/annotation2366470/range2366470/1

https://synbiohub.org/public/igem/BBa_K1067006/annotation2366470/range2366470/1
type: Range
persistent identity: range2366470
display id: range2366470
version: 1
direction: direction/forward
top level: BBa_K1067006
start: 2069
end: 2428
orientation: inline

Heme d1 biosynthesis protein
type: SequenceAnnotation
persistent identity: annotation2366472
display id: annotation2366472
version: 1
top level: BBa_K1067006
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1067006/annotation2366472/range2366472/1

https://synbiohub.org/public/igem/BBa_K1067006/annotation2366472/range2366472/1
type: Range
persistent identity: range2366472
display id: range2366472
version: 1
direction: direction/forward
top level: BBa_K1067006
start: 2425
end: 3603
orientation: inline

c-type cytochrome
type: SequenceAnnotation
persistent identity: annotation2366478
display id: annotation2366478
version: 1
top level: BBa_K1067006
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1067006/annotation2366478/range2366478/1

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type: Range
persistent identity: range2366478
display id: range2366478
version: 1
direction: direction/forward
top level: BBa_K1067006
start: 7509
end: 8990
orientation: inline

Heme d1 biosynthesis protein
type: SequenceAnnotation
persistent identity: annotation2366476
display id: annotation2366476
version: 1
top level: BBa_K1067006
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1067006/annotation2366476/range2366476/1

https://synbiohub.org/public/igem/BBa_K1067006/annotation2366476/range2366476/1
type: Range
persistent identity: range2366476
display id: range2366476
version: 1
direction: direction/forward
top level: BBa_K1067006
start: 5505
end: 6668
orientation: inline

BBa_K1067005
type: ComponentDefinition
persistent identity: BBa K1067005
display id: BBa_K1067005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Nitrification system
creator: Kristian Davidsen and Helen Cook
created: 2013-09-23T11:00:00Z
modified: 2015-05-08T01:09:00Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1376_
m user id: 0
owner id: 16028
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: llll
mutable notes: llll
mutable provenance: llll
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_K1067003
component: BBa_K1067004
component: BBa_K1067002
sequence annotation: BBa_K1067003
sequence annotation: BBa_K1067004
sequence annotation: BBa_K1067002
sequence: https://synbiohub.org/public/igem/BBa_K1067005_sequence/1

BBa_K1067003
type: Component
persistent identity: component2359743
display id: component2359743
version: 1
top level: BBa_K1067005
access: public
definition: BBa_K1067003

BBa_K1067004
type: Component
persistent identity: component2359750
display id: component2359750
version: 1
top level: BBa_K1067005
access: public
definition: BBa_K1067004

BBa_K1067002
type: Component
persistent identity: component2359733
display id: component2359733
version: 1
top level: BBa_K1067005
access: public
definition: BBa_K1067002

BBa_K1067003
type: SequenceAnnotation
persistent identity: annotation2359743
display id: annotation2359743
version: 1
top level: BBa_K1067005
location: https://synbiohub.org/public/igem/BBa_K1067005/annotation2359743/range2359743/1
component: BBa_K1067003

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type: Range
persistent identity: range2359743
display id: range2359743
version: 1
direction: direction/reverse
top level: BBa_K1067005
start: 1448
end: 4521
orientation: inline

BBa_K1067004
type: SequenceAnnotation
persistent identity: annotation2359750
display id: annotation2359750
version: 1
top level: BBa_K1067005
location: https://synbiohub.org/public/igem/BBa_K1067005/annotation2359750/range2359750/1
component: BBa_K1067004

https://synbiohub.org/public/igem/BBa_K1067005/annotation2359750/range2359750/1
type: Range
persistent identity: range2359750
display id: range2359750
version: 1
direction: direction/reverse
top level: BBa_K1067005
start: 4528
end: 7346
orientation: inline

BBa_K1067002
type: SequenceAnnotation
persistent identity: annotation2359733
display id: annotation2359733
version: 1
top level: BBa_K1067005
location: https://synbiohub.org/public/igem/BBa_K1067005/annotation2359733/range2359733/1
component: BBa_K1067002

https://synbiohub.org/public/igem/BBa_K1067005/annotation2359733/range2359733/1
type: Range
persistent identity: range2359733
display id: range2359733
version: 1
direction: direction/reverse
top level: BBa_K1067005
start: 1
end: 1441
orientation: inline

BBa_K1497100
type: ComponentDefinition
persistent identity: BBa K1497100
display id: BBa_K1497100
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Test Page
creator: Sven Jager
created: 2014-09-24T11:00:00Z
modified: 2015-05-08T01:10:46Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1877_
m user id: 0
owner id: 12512
owning group id: 9
sample status: No part sequence
status: status/Planning
bookmark: false
mutable description: fhhfhhf
mutable notes: ztdztd
mutable provenance: hd
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316

BBa_K118017
type: ComponentDefinition
persistent identity: BBa K118017
display id: BBa_K118017
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: rbs+glgC (ADP-glucose pyrophosphorylase)
creator: Andrew Hall
created: 2008-10-05T11:00:00Z
modified: 2015-05-08T01:09:37Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _192_
m user id: 0
owner id: 3282
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: This is the coding sequence of ''glgC'' from ''Escherichia coli'' JM109 with added ribosome binding site (rbs). It encodes ADP-glucose pyrophosphorylase, which catalyses Glucose 1-phosphate + ATP -> ADP-glucose + PPi, a step in the production of glycogen from glucose.
mutable notes: No special considerations.
mutable provenance: Escherichia coli JM109 genomic DNA.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_K118015
component: BBa_J15001
sequence annotation: BBa_J15001
sequence annotation: BBa_K118015
sequence: https://synbiohub.org/public/igem/BBa_K118017_sequence/1

BBa_K118015
type: Component
persistent identity: component1978630
display id: component1978630
version: 1
top level: BBa_K118017
access: public
definition: BBa_K118015

BBa_J15001
type: Component
persistent identity: component1978627
display id: component1978627
version: 1
top level: BBa_K118017
access: public
definition: https://synbiohub.org/public/igem/BBa_J15001/1

BBa_J15001
type: SequenceAnnotation
persistent identity: annotation1978627
display id: annotation1978627
version: 1
top level: BBa_K118017
location: https://synbiohub.org/public/igem/BBa_K118017/annotation1978627/range1978627/1
component: BBa_J15001

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type: Range
persistent identity: range1978627
display id: range1978627
version: 1
direction: direction/reverse
top level: BBa_K118017
start: 1
end: 10
orientation: inline

BBa_K118015
type: SequenceAnnotation
persistent identity: annotation1978630
display id: annotation1978630
version: 1
top level: BBa_K118017
location: https://synbiohub.org/public/igem/BBa_K118017/annotation1978630/range1978630/1
component: BBa_K118015

https://synbiohub.org/public/igem/BBa_K118017/annotation1978630/range1978630/1
type: Range
persistent identity: range1978630
display id: range1978630
version: 1
direction: direction/reverse
top level: BBa_K118017
start: 17
end: 1315
orientation: inline

BBa_K274100
type: ComponentDefinition
persistent identity: BBa K274100
display id: BBa_K274100
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CrtEBI with rbs
creator: Siming Ma
created: 2009-09-17T11:00:00Z
modified: 2015-05-08T01:11:45Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _374_
m user id: 0
owner id: 5416
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This Composite Biobrick is created by standard assembly of Part BBa_K118014 (CrtE with rbs), Part BBa_K118006 (CrtB with rbs) and Part BBa_K118005 (CrtI with rbs), which are submitted by previous iGEM teams. The whole cassette is on plasmid pSB1A2 (high copy, Ampicillin resistance). Together, enzymes CrtE, CrtB and CrtI convert colourless farnesyl pyrophosphate to red lycopene (via intermediates geranylgeranyl pyroiphosphate and phytoene). The red lycopene pigment can then be used as a coloured signal output, e.g. for biosensors. There is already individual ribosome binding site before each enzyme gene sequence. Internal restriction sites have been removed by previous iGEM teams. Please refer to Parts BBa_K118014, BBa_K118006 and BBa_K118005 for more information on individual Biobricks components. This Composite Biobrick has been put under constitutive promoter R0011 (see Part BBa_K274110) and arabinose-inducible promoter I0500 (see Part BBa_274120), transformed and tested in E.coli strain MG1655. Amount of lycopene produced can be measured by photospectrometer with absorbance at 475nm (lycopene extraction using acetone).
mutable notes: The colourless precursor, farnesyl pyrophosphate, is naturally produced in E.coli using Non-mevalonate Pathway (from pyruvate and glyceraldehyde-3-phosphate). Our experiences showed that the Composite Biobrick, when put under a promoter, works better in E.coli strains with up-regulated level of precursors. E.coli strain MG1655 demonostrates higher level of pigment (lycopene) production than strain TOP10.
mutable provenance: Gene coding sequences come from Pantoea ananatis (formerly Erwinia uredovora) (Accession number D90087), a type of Gram negative Enterobacteriaceae. This Composite Biobrick is created by standard assembly of Part BBa_K118014, Part BBa_K118006 and Part BBa_K118005.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K118002
component: BBa_K118012
component: BBa_J15001
component: BBa_I742151
component: BBa_K118003
sequence annotation: BBa_J15001
sequence annotation: BBa_K118012
sequence annotation: BBa_I742151
sequence annotation: BBa_K118002
sequence annotation: BBa_K118003
sequence: https://synbiohub.org/public/igem/BBa_K274100_sequence/1

BBa_K118002
type: Component
persistent identity: component2021354
display id: component2021354
version: 1
top level: BBa_K274100
access: public
definition: https://synbiohub.org/public/igem/BBa_K118002/1

BBa_K118012
type: Component
persistent identity: component2021348
display id: component2021348
version: 1
top level: BBa_K274100
access: public
definition: https://synbiohub.org/public/igem/BBa_K118012/1

BBa_J15001
type: Component
persistent identity: component2021352
display id: component2021352
version: 1
top level: BBa_K274100
access: public
definition: https://synbiohub.org/public/igem/BBa_J15001/1

BBa_J15001
type: Component
persistent identity: component2021357
display id: component2021357
version: 1
top level: BBa_K274100
access: public
definition: https://synbiohub.org/public/igem/BBa_J15001/1

BBa_I742151
type: Component
persistent identity: component2021349
display id: component2021349
version: 1
top level: BBa_K274100
access: public
definition: https://synbiohub.org/public/igem/BBa_I742151/1

BBa_K118003
type: Component
persistent identity: component2021361
display id: component2021361
version: 1
top level: BBa_K274100
access: public
definition: https://synbiohub.org/public/igem/BBa_K118003/1

BBa_J15001
type: SequenceAnnotation
persistent identity: annotation2021357
display id: annotation2021357
version: 1
top level: BBa_K274100
location: https://synbiohub.org/public/igem/BBa_K274100/annotation2021357/range2021357/1
component: BBa_J15001

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type: Range
persistent identity: range2021357
display id: range2021357
version: 1
direction: direction/reverse
top level: BBa_K274100
start: 1888
end: 1897
orientation: inline

BBa_K118012
type: SequenceAnnotation
persistent identity: annotation2021348
display id: annotation2021348
version: 1
top level: BBa_K274100
location: https://synbiohub.org/public/igem/BBa_K274100/annotation2021348/range2021348/1
component: BBa_K118012

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type: Range
persistent identity: range2021348
display id: range2021348
version: 1
direction: direction/reverse
top level: BBa_K274100
start: 1
end: 4
orientation: inline

BBa_I742151
type: SequenceAnnotation
persistent identity: annotation2021349
display id: annotation2021349
version: 1
top level: BBa_K274100
location: https://synbiohub.org/public/igem/BBa_K274100/annotation2021349/range2021349/1
component: BBa_I742151

https://synbiohub.org/public/igem/BBa_K274100/annotation2021349/range2021349/1
type: Range
persistent identity: range2021349
display id: range2021349
version: 1
direction: direction/reverse
top level: BBa_K274100
start: 11
end: 922
orientation: inline

BBa_J15001
type: SequenceAnnotation
persistent identity: annotation2021352
display id: annotation2021352
version: 1
top level: BBa_K274100
location: https://synbiohub.org/public/igem/BBa_K274100/annotation2021352/range2021352/1
component: BBa_J15001

https://synbiohub.org/public/igem/BBa_K274100/annotation2021352/range2021352/1
type: Range
persistent identity: range2021352
display id: range2021352
version: 1
direction: direction/reverse
top level: BBa_K274100
start: 931
end: 940
orientation: inline

BBa_K118002
type: SequenceAnnotation
persistent identity: annotation2021354
display id: annotation2021354
version: 1
top level: BBa_K274100
location: https://synbiohub.org/public/igem/BBa_K274100/annotation2021354/range2021354/1
component: BBa_K118002

https://synbiohub.org/public/igem/BBa_K274100/annotation2021354/range2021354/1
type: Range
persistent identity: range2021354
display id: range2021354
version: 1
direction: direction/reverse
top level: BBa_K274100
start: 947
end: 1879
orientation: inline

BBa_K118003
type: SequenceAnnotation
persistent identity: annotation2021361
display id: annotation2021361
version: 1
top level: BBa_K274100
location: https://synbiohub.org/public/igem/BBa_K274100/annotation2021361/range2021361/1
component: BBa_K118003

https://synbiohub.org/public/igem/BBa_K274100/annotation2021361/range2021361/1
type: Range
persistent identity: range2021361
display id: range2021361
version: 1
direction: direction/reverse
top level: BBa_K274100
start: 1904
end: 3385
orientation: inline

rbs+sam8
type: ComponentDefinition
persistent identity: BBa I742148
display id: BBa_I742148
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: sam8 (tyrosine ammonia lyase) with native rbs
creator: sarah hollingshead
created: 2007-10-23T11:00:00Z
modified: 2015-08-31T04:08:03Z
discontinued: false
dominant: false
experience: experience/None
group u list: _123_
m user id: 0
owner id: 837
owning group id: 163
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This is sam8 (tyrosine ammonia lyase) of the bacterium Saccharothrix espanaensis DSM 44229 with its native ribosome binding site.
mutable notes: This part was made as a single biobrick rather than separate rbs and coding sequence, hence the use of blunt ligation in generating the final sequence.
mutable provenance: Saccharothrix espanaensis DSM 44229
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_I742142
component: BBa_I742146
sequence annotation: BBa_I742142
sequence annotation: BBa_I742146
sequence: https://synbiohub.org/public/igem/BBa_I742148_sequence/1

BBa_I742142
type: Component
persistent identity: component1953824
display id: component1953824
version: 1
top level: rbs+sam8
access: public
definition: sam8

BBa_I742146
type: Component
persistent identity: component1953823
display id: component1953823
version: 1
top level: rbs+sam8
access: public
definition: https://synbiohub.org/public/igem/BBa_I742146/1

BBa_I742142
type: SequenceAnnotation
persistent identity: annotation1953824
display id: annotation1953824
version: 1
top level: rbs+sam8
location: https://synbiohub.org/public/igem/BBa_I742148/annotation1953824/range1953824/1
component: BBa_I742142

https://synbiohub.org/public/igem/BBa_I742148/annotation1953824/range1953824/1
type: Range
persistent identity: range1953824
display id: range1953824
version: 1
direction: direction/reverse
top level: rbs+sam8
start: 15
end: 1550
orientation: inline

BBa_I742146
type: SequenceAnnotation
persistent identity: annotation1953823
display id: annotation1953823
version: 1
top level: rbs+sam8
location: https://synbiohub.org/public/igem/BBa_I742148/annotation1953823/range1953823/1
component: BBa_I742146

https://synbiohub.org/public/igem/BBa_I742148/annotation1953823/range1953823/1
type: Range
persistent identity: range1953823
display id: range1953823
version: 1
direction: direction/reverse
top level: rbs+sam8
start: 1
end: 14
orientation: inline

cry11Aa
type: ComponentDefinition
persistent identity: BBa K332011
display id: BBa_K332011
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Crystal protein gene (cry11Aa) from Bacillus thuringiensis subsp. Israelensis.
creator: Chia-Le Meng
created: 2010-10-23T11:00:00Z
modified: 2015-05-08T01:12:05Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _452_
m user id: 0
owner id: 7487
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: The cry weapon system produce crystal protein, targetting the wrigglers, larvae of mosquitoes. The cry11Aa gene is cloned from Bacillus thuringiensis subsp. Israelensis. The cry protein is controlled by the tetR-repressible promoter PtetR (BBa_R0040), which in turn is regulated by a temperature control system. When E.coli is released into environment (<37C), tetR begins to degrade, resulting in the promoter PtetR (BBa_R0011) expressing downstream genes, cry11Aa (BBa_K332012) and Green Fluorescent Protein (GFP)(BBa_E0040).
mutable notes: Procedures (I) Culture Bti. Bacillus thuringiensis subsp. Israelensis (BCRC15860) 1. Prepare agar plate for Bti. Beef extract 3.0g Peptone 5.0g Agar 15.0g ddH2O 1 L *adjust PH to 7.0 *No antibiotic *Be aware of contamination 2. Plate Bti. on the medium and incubate the cultures at 30??C overnight. (II) Clone cry11Aa from Bti. into TA vector 1. Extract genomic DNA of Bti. by liquid nitrogen. 2. Add some ddH2O to dilute the DNA. 3. Design primers Vf2: ATTCAATAAAAGGTGGAATGAATTATGGA Tm: 53??C VR: GTGCTAACATGACTTCTACTTTAGT Tm: 52.8??C 4. Find the best PCR condition by gradient PCR. Anneling temperature: 51??C??10??C 5. PCR by B-taq plus on the best condition Template DNA（10ng/μl） 2.0 B-taq buffer 5.0 dNTP（2mM） 5.0 forward primer（10μM） 1.5 reverse primer（10μM） 1.5 B-taq plus DNA polymerase（2Kb） 1.0 ddH2O 34.0 Total 50 μl 6. Digestion to confirm the cry11Aa fragment and enzyme sites. 7. TA clone 8. DNA sequencing (III) PCR mutagenesis at two enzyme sites --- EcoR1 and Spe1 1. Design primers by primerX. EcoR1-Vf2: CGG GTA CAA TCT CAG AAC TCG GGA AAT AAT AGA A EcoR1-VR: TTC TAT TAT TTC CCG AGT TCT GAG ATT GTA CCC G Spe1-Vf2: ATA ATG AAT GGG GAG GAC TGG TTT ATA AGT TAT TAA TGG G Spe1-VR: CTT CCC CCA TTA ATA ACT TAT AAA CTA GTC CTC CCC ATT CAT 2. Digestion to confirm the fragments (IV) PCR construction of Biobrick parts 1. Design primers by Assembly standard 10. Vf2: GTTTCTTC GAATTC GCGGCCGC T TCTAG ATGGAAGATAGTTCTTTAGATACT VR: GTTTCTTC CTGCAG CGGCCGC T ACTAGT A CTACTTTAGTAACGGATT 2. PCR condition 3. Ligation to backbones(Psb1C3). (V) Transform into E.coli 1. Thaw competent cells and BBa_K332012 plasmid on ice. 2. Add 2ul plasmid to competent cell and place in ice for 5 minutes. 3. Put the transformed cells into 42℃ water bath for 45 seconds. 4. Plate the cells on the appropriate media and antibiotic, such as agar plates with 25 ??g/ml kanamycin. 5. Incubate the cultures at 37??C overnight. (VI) Culture with mosquito larvae and observe
mutable provenance: Crystal protein gene (cry11Aa) from Bacillus thuringiensis subsp. Israelensis. BCRC Number: 15860 Organism: Bacillus thuringiensis subsp. israelensis de Barjac History: << ATCC << USDA << ONR 60 << H. de Barjac << L. J. Goldberg 60 Other Collection No.: ATCC 35646 Source: sewage, Israel Characterization: Production of delta-endotoxin against dipteran larvae --B3009 Others: referred to as the type strain --B3008 Growth Conditions: 30??C , Medium ID: 3 Oxygen Requirement: Aerobic Biosafety Level: 1 References: Temeyer, K. B. 1984. Larvicidal activity of Bacillus thuringiensis subsp. israelensis in the dipteran Haematobia irritans. Appl. Environ. Microbiol.47 : 952-955. PMID: 6742837. Demezas, D. H. and J. Bell. 1995. Evaluation of low molecular weight RNA profiles and ribotyping to differentiate some Bacillus species. Syst. Appl. Microbiol.18 : 582-589.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K332011_sequence/1

BisdA
type: ComponentDefinition
persistent identity: BBa K123000
display id: BBa_K123000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: BisdA
creator: Jason Gardiner
created: 2008-10-27T12:00:00Z
modified: 2015-05-08T01:09:44Z
discontinued: false
dominant: false
experience: experience/None
group u list: _241_
m user id: 0
owner id: 2742
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: When used in conjunction with BisdB this enzyme can be used to degrade Bisphenol A. Activity has previously been recorded in E.coli by Miho Sasaki1 et al. 2005. Miho Sasaki1, Jun-ichi Maki, Ko-ichi Oshiman, Yoshinobu Matsumura1, and Tetsuaki Tsuchido.2005, Biodegradation of bisphenol A by cells and cell lysate from Sphingomonas sp. strain AO1. Biodegredation 16: 449-459
mutable notes: These parts where designed to include a his tag to make it easier to view enzyme presence
mutable provenance: Sphingomonas sp. strain AO1
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K123000_sequence/1

BBa_K081009
type: ComponentDefinition
persistent identity: BBa K081009
display id: BBa_K081009
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: lasI protein generator (TERM-)
creator: Lorenzo Pasotti, Paolo Magni
created: 2008-10-17T11:00:00Z
modified: 2015-05-08T01:08:35Z
discontinued: false
dominant: false
experience: experience/None
group u list: _227_
m user id: 0
owner id: 2583
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: lasI: autoinducer synthetase for PAI from Pseudomonas aeruginosa. Strong RBS (efficiency=0.6).
mutable notes: We used BioBrick Standard Assembly.
mutable provenance: lasI: RBS:
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_C0078
component: BBa_B0030
sequence annotation: BBa_C0078
sequence annotation: BBa_B0030
sequence: https://synbiohub.org/public/igem/BBa_K081009_sequence/1

BBa_C0078
type: Component
persistent identity: component2244000
display id: component2244000
version: 1
top level: BBa_K081009
access: public
definition: https://synbiohub.org/public/igem/BBa_C0078/1

BBa_B0030
type: Component
persistent identity: component2243995
display id: component2243995
version: 1
top level: BBa_K081009
access: public
definition: https://synbiohub.org/public/igem/BBa_B0030/1

BBa_C0078
type: SequenceAnnotation
persistent identity: annotation2244000
display id: annotation2244000
version: 1
top level: BBa_K081009
location: https://synbiohub.org/public/igem/BBa_K081009/annotation2244000/range2244000/1
component: BBa_C0078

https://synbiohub.org/public/igem/BBa_K081009/annotation2244000/range2244000/1
type: Range
persistent identity: range2244000
display id: range2244000
version: 1
direction: direction/reverse
top level: BBa_K081009
start: 22
end: 688
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation2243995
display id: annotation2243995
version: 1
top level: BBa_K081009
location: https://synbiohub.org/public/igem/BBa_K081009/annotation2243995/range2243995/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_K081009/annotation2243995/range2243995/1
type: Range
persistent identity: range2243995
display id: range2243995
version: 1
direction: direction/forward
top level: BBa_K081009
start: 1
end: 15
orientation: inline

PyrF
type: ComponentDefinition
persistent identity: BBa T9150
display id: BBa_T9150
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: orotidine 5
creator: Jessica Harpole
created: 2005-08-22T11:00:00Z
modified: 2015-05-08T01:14:53Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_1_
m user id: 0
owner id: 411
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Coding region for PyrF gene. PyrF is used in the synthesis of uracil in the same pathway that Ura3 is used in yeast. Can also be used as a conterselectable marker in the presence of 5-Fluoroorotic Acid (5-FOA) and the absence of uracil.
mutable notes: Four silent mutations were introduced to eliminate EcoRI and PstI restriction enzyme sites. The EcoRI site was eliminated at base 39 with a G -> A mutation. PstI sites were eliminated at 240 and and 441 with T -> C mutations, and at 261 with a T -> G mutation. Mutation choices were selected to match codons with similar codon usage.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: PyrF
sequence annotation: G
sequence annotation: T
sequence: https://synbiohub.org/public/igem/BBa_T9150_sequence/1

PyrF
type: SequenceAnnotation
persistent identity: annotation1892252
display id: annotation1892252
version: 1
top level: PyrF
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_T9150/annotation1892252/range1892252/1

https://synbiohub.org/public/igem/BBa_T9150/annotation1892252/range1892252/1
type: Range
persistent identity: range1892252
display id: range1892252
version: 1
direction: direction/forward
top level: PyrF
start: 1
end: 741
orientation: inline

G
type: SequenceAnnotation
persistent identity: annotation1666362
display id: annotation1666362
version: 1
top level: PyrF
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_T9150/annotation1666362/range1666362/1

https://synbiohub.org/public/igem/BBa_T9150/annotation1666362/range1666362/1
type: Range
persistent identity: range1666362
display id: range1666362
version: 1
direction: direction/forward
top level: PyrF
start: 39
end: 44
orientation: inline

T
type: SequenceAnnotation
persistent identity: annotation1666373
display id: annotation1666373
version: 1
top level: PyrF
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_T9150/annotation1666373/range1666373/1

https://synbiohub.org/public/igem/BBa_T9150/annotation1666373/range1666373/1
type: Range
persistent identity: range1666373
display id: range1666373
version: 1
direction: direction/forward
top level: PyrF
start: 441
end: 446
orientation: inline

T
type: SequenceAnnotation
persistent identity: annotation1666364
display id: annotation1666364
version: 1
top level: PyrF
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_T9150/annotation1666364/range1666364/1

https://synbiohub.org/public/igem/BBa_T9150/annotation1666364/range1666364/1
type: Range
persistent identity: range1666364
display id: range1666364
version: 1
direction: direction/forward
top level: PyrF
start: 261
end: 266
orientation: inline

T
type: SequenceAnnotation
persistent identity: annotation1666363
display id: annotation1666363
version: 1
top level: PyrF
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_T9150/annotation1666363/range1666363/1

https://synbiohub.org/public/igem/BBa_T9150/annotation1666363/range1666363/1
type: Range
persistent identity: range1666363
display id: range1666363
version: 1
direction: direction/forward
top level: PyrF
start: 240
end: 245
orientation: inline

BBa_K1554003
type: ComponentDefinition
persistent identity: BBa K1554003
display id: BBa_K1554003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: EaDAcT
creator: Valencia_UPV team members
created: 2014-09-25T11:00:00Z
modified: 2015-05-28T11:29:58Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1939_
m user id: 4206
owner id: 22386
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: diacylglycerol acetyltransferase
mutable notes: BioBrick compatible
mutable provenance: It comes from Euonymus alatus.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1554003_sequence/1

BBa_K118015
type: ComponentDefinition
persistent identity: BBa K118015
display id: BBa_K118015
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: glgC coding sequence encoding ADP-glucose pyrophosphorylase
creator: Andrew Hall
created: 2008-10-05T11:00:00Z
modified: 2015-05-08T01:09:37Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _192_
m user id: 0
owner id: 3282
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: This is the coding sequence of ''glgC'' from ''Escherichia coli'' JM109. It encodes ADP-glucose pyrophosphorylase, which catalyses Glucose 1-phosphate + ATP -> ADP-glucose + PPi, a step in the production of glycogen from glucose.
mutable notes: The genomic DNA sequence contains to EcoRI sites, both of which have been mutated out using the mutagenesis by blunt-ended ligation (MABEL) technique
mutable provenance: ''Escherichia coli'' JM109 genomic DNA.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: EcoRI site removed
sequence: https://synbiohub.org/public/igem/BBa_K118015_sequence/1

EcoRI site removed
type: SequenceAnnotation
persistent identity: annotation1978555
display id: annotation1978555
version: 1
top level: BBa_K118015
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_K118015/annotation1978555/range1978555/1

https://synbiohub.org/public/igem/BBa_K118015/annotation1978555/range1978555/1
type: Range
persistent identity: range1978555
display id: range1978555
version: 1
direction: direction/forward
top level: BBa_K118015
start: 571
end: 576
orientation: inline

EcoRI site removed
type: SequenceAnnotation
persistent identity: annotation1978556
display id: annotation1978556
version: 1
top level: BBa_K118015
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_K118015/annotation1978556/range1978556/1

https://synbiohub.org/public/igem/BBa_K118015/annotation1978556/range1978556/1
type: Range
persistent identity: range1978556
display id: range1978556
version: 1
direction: direction/forward
top level: BBa_K118015
start: 1068
end: 1073
orientation: inline

BBa_K1067004
type: ComponentDefinition
persistent identity: BBa K1067004
display id: BBa_K1067004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Hydroxylamine oxidoreductase
creator: Kristian Davidsen and Helen Cook
created: 2013-09-22T11:00:00Z
modified: 2015-05-08T01:09:00Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1376_
m user id: 0
owner id: 16028
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: ffff
mutable notes: fff
mutable provenance: fff
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Putative protein in samme operon
sequence annotation: start
sequence annotation: HAO
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_K1067004_sequence/1

Putative protein in samme operon
type: SequenceAnnotation
persistent identity: annotation2359526
display id: annotation2359526
version: 1
top level: BBa_K1067004
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1067004/annotation2359526/range2359526/1

https://synbiohub.org/public/igem/BBa_K1067004/annotation2359526/range2359526/1
type: Range
persistent identity: range2359526
display id: range2359526
version: 1
direction: direction/forward
top level: BBa_K1067004
start: 1710
end: 2819
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2359529
display id: annotation2359529
version: 1
top level: BBa_K1067004
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1067004/annotation2359529/range2359529/1

https://synbiohub.org/public/igem/BBa_K1067004/annotation2359529/range2359529/1
type: Range
persistent identity: range2359529
display id: range2359529
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