//dna/spacer
type: Collection
persistent identity: dna spacer
display id: dna_spacer
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
owned by: james
owned by: myers
member: https://synbiohub.org/public/igem/BBa_B0040/1
member: https://synbiohub.org/public/igem/BBa_K259002/1
member: https://synbiohub.org/public/igem/BBa_K259008/1
member: https://synbiohub.org/public/igem/BBa_K259009/1
member: https://synbiohub.org/public/igem/BBa_K259004/1

//dna/conjugation
type: Collection
persistent identity: dna conjugation
display id: dna_conjugation
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
owned by: james
owned by: myers
member: https://synbiohub.org/public/igem/BBa_K125320/1
member: https://synbiohub.org/public/igem/BBa_J01003/1
member: https://synbiohub.org/public/igem/BBa_K125360/1
member: https://synbiohub.org/public/igem/BBa_K175000/1
member: https://synbiohub.org/public/igem/BBa_I714031/1
member: https://synbiohub.org/public/igem/BBa_I714030/1
member: https://synbiohub.org/public/igem/BBa_K175001/1
member: https://synbiohub.org/public/igem/BBa_J01002/1
member: https://synbiohub.org/public/igem/BBa_I714032/1
member: https://synbiohub.org/public/igem/BBa_K125350/1

//dna/nucleotide
type: Collection
persistent identity: dna nucleotide
display id: dna_nucleotide
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
owned by: james
owned by: myers
member: https://synbiohub.org/public/igem/BBa_G0010/1
member: https://synbiohub.org/public/igem/BBa_G0012/1
member: https://synbiohub.org/public/igem/BBa_G0011/1
member: https://synbiohub.org/public/igem/BBa_G0009/1
member: https://synbiohub.org/public/igem/BBa_K649204/1
member: https://synbiohub.org/public/igem/BBa_M34014/1
member: https://synbiohub.org/public/igem/BBa_K361001/1

//dna/origami
type: Collection
persistent identity: dna origami
display id: dna_origami
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
owned by: james
owned by: myers
member: https://synbiohub.org/public/igem/BBa_J35004/1
member: https://synbiohub.org/public/igem/BBa_J35000/1
member: https://synbiohub.org/public/igem/BBa_J35007/1
member: https://synbiohub.org/public/igem/BBa_J35120/1
member: https://synbiohub.org/public/igem/BBa_J35002/1
member: https://synbiohub.org/public/igem/BBa_K1479005/1
member: https://synbiohub.org/public/igem/BBa_J35001/1
member: https://synbiohub.org/public/igem/BBa_J35003/1
member: https://synbiohub.org/public/igem/BBa_J35005/1
member: https://synbiohub.org/public/igem/BBa_J35006/1

//dna/bioscaffold
type: Collection
persistent identity: dna bioscaffold
display id: dna_bioscaffold
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
owned by: james
owned by: myers
member: https://synbiohub.org/public/igem/BBa_K259002/1
member: https://synbiohub.org/public/igem/BBa_J70030/1
member: https://synbiohub.org/public/igem/BBa_J70034/1
member: https://synbiohub.org/public/igem/BBa_J70032/1
member: https://synbiohub.org/public/igem/BBa_K259009/1
member: https://synbiohub.org/public/igem/BBa_J70012/1
member: https://synbiohub.org/public/igem/BBa_J70052/1
member: https://synbiohub.org/public/igem/BBa_J70050/1
member: https://synbiohub.org/public/igem/BBa_K606026/1
member: https://synbiohub.org/public/igem/BBa_J70036/1
member: https://synbiohub.org/public/igem/BBa_K259008/1
member: https://synbiohub.org/public/igem/BBa_K259004/1
member: https://synbiohub.org/public/igem/BBa_J70040/1
member: https://synbiohub.org/public/igem/BBa_J70010/1

//dna
type: Collection
persistent identity: dna
display id: dna
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
owned by: james
owned by: myers
member: O3
member: Orai1 V102
member: O4
member: IscA1
member: //dna/nucleotide
member: //dna/chromosomalintegration
member: BBa_K1614009
member: //dna/cloningsite
member: BBa_K1680005
member: //dna/spacer
member: Tetrahedro
member: //dna/restrictionsite
member: O5
member: BBa_K1614013
member: BBa_K1680014
member: IscA2
member: //dna/transposon
member: BBa_K1680015
member: MDA
member: //dna/origami
member: O1
member: //dna/conjugation
member: //dna/transposome
member: BBa_K1614007
member: Active Com
member: BBa_K1680016
member: O2
member: //dna/origin_of_replication
member: //dna/aptamer
member: //dna/primerbindingsite
member: //dna/bioscaffold
member: //dna/scar

//dna/cloningsite
type: Collection
persistent identity: dna cloningsite
display id: dna_cloningsite
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
owned by: james
owned by: myers
member: https://synbiohub.org/public/igem/BBa_I732007/1
member: https://synbiohub.org/public/igem/BBa_I13452/1
member: https://synbiohub.org/public/igem/BBa_J56011/1
member: https://synbiohub.org/public/igem/BBa_I13456/1
member: https://synbiohub.org/public/igem/BBa_I13455/1
member: https://synbiohub.org/public/igem/BBa_I13457/1
member: https://synbiohub.org/public/igem/BBa_G00001/1
member: https://synbiohub.org/public/igem/BBa_G00000/1
member: https://synbiohub.org/public/igem/BBa_I13450/1

//dna/primerbindingsite
type: Collection
persistent identity: dna primerbindingsite
display id: dna_primerbindingsite
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
owned by: james
owned by: myers
member: https://synbiohub.org/public/igem/BBa_G00102/1
member: https://synbiohub.org/public/igem/BBa_G00001/1
member: https://synbiohub.org/public/igem/BBa_G00100/1
member: https://synbiohub.org/public/igem/BBa_G00000/1

//dna/scar
type: Collection
persistent identity: dna scar
display id: dna_scar
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
owned by: james
owned by: myers
member: https://synbiohub.org/public/igem/BBa_K106015/1
member: https://synbiohub.org/public/igem/BBa_G0002/1
member: https://synbiohub.org/public/igem/BBa_B0105/1
member: https://synbiohub.org/public/igem/BBa_K245009/1
member: https://synbiohub.org/public/igem/BBa_B0104/1
member: https://synbiohub.org/public/igem/BBa_G0003/1
member: https://synbiohub.org/public/igem/BBa_K1319107/1
member: https://synbiohub.org/public/igem/BBa_G0005/1
member: https://synbiohub.org/public/igem/BBa_K106016/1
member: https://synbiohub.org/public/igem/BBa_G0001/1
member: https://synbiohub.org/public/igem/BBa_K112999/1
member: https://synbiohub.org/public/igem/BBa_G0000/1
member: https://synbiohub.org/public/igem/BBa_G0006/1
member: https://synbiohub.org/public/igem/BBa_K1319106/1
member: https://synbiohub.org/public/igem/BBa_G0004/1
member: https://synbiohub.org/public/igem/BBa_K106017/1

//dna/transposon
type: Collection
persistent identity: dna transposon
display id: dna_transposon
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
owned by: james
owned by: myers
member: https://synbiohub.org/public/igem/BBa_K510000/1
member: https://synbiohub.org/public/igem/BBa_J61021/1
member: https://synbiohub.org/public/igem/BBa_J61022/1
member: https://synbiohub.org/public/igem/BBa_K1973006/1

//dna/transposome
type: Collection
persistent identity: dna transposome
display id: dna_transposome
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
owned by: james
owned by: myers
member: https://synbiohub.org/public/igem/dna_transposome_tn5/1

//dna/origin_of_replication
type: Collection
persistent identity: dna origin of replication
display id: dna_origin_of_replication
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
owned by: james
owned by: myers
member: https://synbiohub.org/public/igem/BBa_K809002/1

//dna/restrictionsite
type: Collection
persistent identity: dna restrictionsite
display id: dna_restrictionsite
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
owned by: james
owned by: myers
member: https://synbiohub.org/public/igem/BBa_I13451/1
member: https://synbiohub.org/public/igem/BBa_M31952/1
member: https://synbiohub.org/public/igem/BBa_M31982/1
member: https://synbiohub.org/public/igem/BBa_B0102/1
member: https://synbiohub.org/public/igem/BBa_B0046/1
member: https://synbiohub.org/public/igem/BBa_M31961/1
member: https://synbiohub.org/public/igem/BBa_M31987/1
member: https://synbiohub.org/public/igem/BBa_M31996/1
member: https://synbiohub.org/public/igem/BBa_I11082/1
member: https://synbiohub.org/public/igem/BBa_K175027/1
member: https://synbiohub.org/public/igem/BBa_I11073/1
member: https://synbiohub.org/public/igem/BBa_M31984/1
member: https://synbiohub.org/public/igem/BBa_M31968/1
member: https://synbiohub.org/public/igem/BBa_M31997/1
member: https://synbiohub.org/public/igem/BBa_M31992/1
member: https://synbiohub.org/public/igem/BBa_I11078/1
member: https://synbiohub.org/public/igem/BBa_I11072/1
member: https://synbiohub.org/public/igem/BBa_M31955/1
member: https://synbiohub.org/public/igem/BBa_M31985/1
member: https://synbiohub.org/public/igem/BBa_M31991/1
member: https://synbiohub.org/public/igem/BBa_B0103/1
member: https://synbiohub.org/public/igem/BBa_M31986/1
member: https://synbiohub.org/public/igem/BBa_I11079/1
member: https://synbiohub.org/public/igem/BBa_K259004/1
member: https://synbiohub.org/public/igem/BBa_I11071/1
member: https://synbiohub.org/public/igem/BBa_M31956/1
member: https://synbiohub.org/public/igem/BBa_M31990/1
member: https://synbiohub.org/public/igem/BBa_M31965/1
member: https://synbiohub.org/public/igem/BBa_K150008/1
member: https://synbiohub.org/public/igem/BBa_M31995/1
member: https://synbiohub.org/public/igem/BBa_K259009/1
member: https://synbiohub.org/public/igem/BBa_M31953/1
member: https://synbiohub.org/public/igem/BBa_I11074/1
member: https://synbiohub.org/public/igem/BBa_M31983/1
member: https://synbiohub.org/public/igem/BBa_M31962/1
member: https://synbiohub.org/public/igem/BBa_B0045/1
member: https://synbiohub.org/public/igem/BBa_M31967/1
member: https://synbiohub.org/public/igem/BBa_K175002/1
member: https://synbiohub.org/public/igem/BBa_M31988/1
member: https://synbiohub.org/public/igem/BBa_M31958/1
member: https://synbiohub.org/public/igem/BBa_K259002/1
member: https://synbiohub.org/public/igem/BBa_M31989/1
member: https://synbiohub.org/public/igem/BBa_K792013/1
member: https://synbiohub.org/public/igem/BBa_I11077/1
member: https://synbiohub.org/public/igem/BBa_M31950/1
member: https://synbiohub.org/public/igem/BBa_I11081/1
member: https://synbiohub.org/public/igem/BBa_B0048/1
member: https://synbiohub.org/public/igem/BBa_I11076/1
member: https://synbiohub.org/public/igem/BBa_B0047/1
member: https://synbiohub.org/public/igem/BBa_K259008/1
member: https://synbiohub.org/public/igem/BBa_M31964/1
member: https://synbiohub.org/public/igem/BBa_I11080/1
member: https://synbiohub.org/public/igem/BBa_I11075/1

//dna/chromosomalintegration
type: Collection
persistent identity: dna chromosomalintegration
display id: dna_chromosomalintegration
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
owned by: james
owned by: myers
member: https://synbiohub.org/public/igem/BBa_K1642006/1
member: https://synbiohub.org/public/igem/BBa_K143002/1
member: https://synbiohub.org/public/igem/BBa_K592200/1
member: https://synbiohub.org/public/igem/BBa_K510000/1
member: https://synbiohub.org/public/igem/BBa_K1930003/1
member: https://synbiohub.org/public/igem/BBa_K1973006/1
member: https://synbiohub.org/public/igem/BBa_K592202/1
member: https://synbiohub.org/public/igem/BBa_K1642005/1
member: https://synbiohub.org/public/igem/BBa_K143001/1
member: https://synbiohub.org/public/igem/BBa_K1930002/1

//dna/aptamer
type: Collection
persistent identity: dna aptamer
display id: dna_aptamer
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
owned by: james
owned by: myers
member: https://synbiohub.org/public/igem/BBa_K1330000/1
member: https://synbiohub.org/public/igem/BBa_K1636000/1
member: https://synbiohub.org/public/igem/BBa_J36854/1
member: https://synbiohub.org/public/igem/BBa_J36856/1
member: https://synbiohub.org/public/igem/BBa_K598001/1
member: https://synbiohub.org/public/igem/BBa_J36859/1
member: https://synbiohub.org/public/igem/BBa_K2027049/1
member: BBa_K1614009
member: https://synbiohub.org/public/igem/BBa_J36857/1
member: https://synbiohub.org/public/igem/BBa_J36855/1
member: https://synbiohub.org/public/igem/BBa_J36858/1
member: https://synbiohub.org/public/igem/BBa_J36853/1
member: BBa_K1614013
member: https://synbiohub.org/public/igem/BBa_K2027050/1

O3
type: ComponentDefinition
persistent identity: BBa K2054003
display id: BBa_K2054003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Oligo 3 of DNA Tetrahedral Nanostructure
creator: LAI HEI WAI
created: 2016-10-13T11:00:00Z
modified: 2016-10-27T11:56:35Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2522_
m user id: 0
m user id: 30316
owner id: 30316
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This will be used to build our desired nanostructure that target micro RNA biomarkers. This plasmid encodes the second ssDNA that will make the tetrahedral nanostructure, together with oligo 1, 2, 4 and 5.
mutable notes: The assembly of the structure.
mutable provenance: Elbaz, J., Yin, P., & Voigt, C. A. (2016). Genetic encoding of DNA nanostructures and their self-assembly in living bacteria. Nature communications, 7.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_K2054003_sequence/1

BBa_K1680014
type: ComponentDefinition
persistent identity: BBa K1680014
display id: BBa_K1680014
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pRS313 yeast shuttle vector with Biobrick MCS
creator: Katharina Sporbeck
created: 2015-09-16T11:00:00Z
modified: 2015-10-27T01:47:18Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2098_
m user id: 15693
owner id: 26460
owning group id: 9
status: status/Planning
bookmark: false
mutable description: A shuttle vector for yeast. The vector is compatible with RFC10 standard and contains an ADH terminator behind the RFC10 cloning site. Ampicilin resistance for bacteria and URA3 gene for yeast selection.
mutable notes: contains an ADH Terminator and restrictions sites for the RFC10 standard only in the multiple cloning site, no restrictions sites for RCF10 enzymes in the backbone.
mutable provenance: yeast shuttle vector
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: lac promotor
sequence annotation: CEN/ARS yeast centromere
sequence annotation: RFC10 MCS
sequence annotation: pUC origin
sequence annotation: T7 promotor
sequence annotation: lacZalpha
sequence annotation: ampR
sequence annotation: HIS3 promotor
sequence annotation: ampR promotor
sequence annotation: f1 ori
sequence annotation: T3 promotor
sequence annotation: HIS3
sequence: https://synbiohub.org/public/igem/BBa_K1680014_sequence/1

lac promotor
type: SequenceAnnotation
persistent identity: annotation2466127
display id: annotation2466127
version: 1
top level: BBa_K1680014
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1680014/annotation2466127/range2466127/1

https://synbiohub.org/public/igem/BBa_K1680014/annotation2466127/range2466127/1
type: Range
persistent identity: range2466127
display id: range2466127
version: 1
direction: direction/reverse
top level: BBa_K1680014
start: 2241
end: 2271
orientation: inline

CEN/ARS yeast centromere
type: SequenceAnnotation
persistent identity: annotation2466131
display id: annotation2466131
version: 1
top level: BBa_K1680014
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1680014/annotation2466131/range2466131/1

https://synbiohub.org/public/igem/BBa_K1680014/annotation2466131/range2466131/1
type: Range
persistent identity: range2466131
display id: range2466131
version: 1
direction: direction/forward
top level: BBa_K1680014
start: 4356
end: 4859
orientation: inline

RFC10 MCS
type: SequenceAnnotation
persistent identity: annotation2466135
display id: annotation2466135
version: 1
top level: BBa_K1680014
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1680014/annotation2466135/range2466135/1

https://synbiohub.org/public/igem/BBa_K1680014/annotation2466135/range2466135/1
type: Range
persistent identity: range2466135
display id: range2466135
version: 1
direction: direction/forward
top level: BBa_K1680014
start: 2083
end: 2125
orientation: inline

pUC origin
type: SequenceAnnotation
persistent identity: annotation2466128
display id: annotation2466128
version: 1
top level: BBa_K1680014
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1680014/annotation2466128/range2466128/1

https://synbiohub.org/public/igem/BBa_K1680014/annotation2466128/range2466128/1
type: Range
persistent identity: range2466128
display id: range2466128
version: 1
direction: direction/reverse
top level: BBa_K1680014
start: 2595
end: 3183
orientation: inline

T7 promotor
type: SequenceAnnotation
persistent identity: annotation2466133
display id: annotation2466133
version: 1
top level: BBa_K1680014
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1680014/annotation2466133/range2466133/1

https://synbiohub.org/public/igem/BBa_K1680014/annotation2466133/range2466133/1
type: Range
persistent identity: range2466133
display id: range2466133
version: 1
direction: direction/forward
top level: BBa_K1680014
start: 2046
end: 2065
orientation: inline

lacZalpha
type: SequenceAnnotation
persistent identity: annotation2466100
display id: annotation2466100
version: 1
top level: BBa_K1680014
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1680014/annotation2466100/range2466100/1

https://synbiohub.org/public/igem/BBa_K1680014/annotation2466100/range2466100/1
type: Range
persistent identity: range2466100
display id: range2466100
version: 1
direction: direction/forward
top level: BBa_K1680014
start: 1661
end: 2077
orientation: inline

ampR
type: SequenceAnnotation
persistent identity: annotation2466129
display id: annotation2466129
version: 1
top level: BBa_K1680014
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1680014/annotation2466129/range2466129/1

https://synbiohub.org/public/igem/BBa_K1680014/annotation2466129/range2466129/1
type: Range
persistent identity: range2466129
display id: range2466129
version: 1
direction: direction/reverse
top level: BBa_K1680014
start: 3354
end: 4214
orientation: inline

HIS3 promotor
type: SequenceAnnotation
persistent identity: annotation2466090
display id: annotation2466090
version: 1
top level: BBa_K1680014
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1680014/annotation2466090/range2466090/1

https://synbiohub.org/public/igem/BBa_K1680014/annotation2466090/range2466090/1
type: Range
persistent identity: range2466090
display id: range2466090
version: 1
direction: direction/forward
top level: BBa_K1680014
start: 315
end: 502
orientation: inline

ampR promotor
type: SequenceAnnotation
persistent identity: annotation2466130
display id: annotation2466130
version: 1
top level: BBa_K1680014
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1680014/annotation2466130/range2466130/1

https://synbiohub.org/public/igem/BBa_K1680014/annotation2466130/range2466130/1
type: Range
persistent identity: range2466130
display id: range2466130
version: 1
direction: direction/reverse
top level: BBa_K1680014
start: 4215
end: 4319
orientation: inline

f1 ori
type: SequenceAnnotation
persistent identity: annotation2466099
display id: annotation2466099
version: 1
top level: BBa_K1680014
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1680014/annotation2466099/range2466099/1

https://synbiohub.org/public/igem/BBa_K1680014/annotation2466099/range2466099/1
type: Range
persistent identity: range2466099
display id: range2466099
version: 1
direction: direction/reverse
top level: BBa_K1680014
start: 1423
end: 1878
orientation: inline

T3 promotor
type: SequenceAnnotation
persistent identity: annotation2466125
display id: annotation2466125
version: 1
top level: BBa_K1680014
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1680014/annotation2466125/range2466125/1

https://synbiohub.org/public/igem/BBa_K1680014/annotation2466125/range2466125/1
type: Range
persistent identity: range2466125
display id: range2466125
version: 1
direction: direction/reverse
top level: BBa_K1680014
start: 2153
end: 2172
orientation: inline

HIS3
type: SequenceAnnotation
persistent identity: annotation2466098
display id: annotation2466098
version: 1
top level: BBa_K1680014
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1680014/annotation2466098/range2466098/1

https://synbiohub.org/public/igem/BBa_K1680014/annotation2466098/range2466098/1
type: Range
persistent identity: range2466098
display id: range2466098
version: 1
direction: direction/forward
top level: BBa_K1680014
start: 503
end: 1162
orientation: inline

Orai1 V102
type: ComponentDefinition
persistent identity: BBa K2028000
display id: BBa_K2028000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: This part is the cDNA of a mutated calcium ion channel protein.
creator: Zhongxiu Hu
created: 2016-10-13T11:00:00Z
modified: 2016-10-29T08:21:59Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2495_
m user id: 30292
owner id: 30292
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This part encodes the gene of Calcium release-activated calcium channel protein 1 (Orai1), which is a part of calcium selective ion channel in humans. Orai1 calcium channels in the plasma membrane are activated by stromal interaction molecule-1 (STIM1), an endoplasmic reticulum calcium sensor, to mediate store-operated calcium entry (SOCE).Store-operated calcium (Ca2+) entry (SOCE) is activated in response to cellular stimuli that deplete the sarco/endoplasmic reticulum (ER) lumen of Ca2+, a prerequisite event that opens exquisitely Ca2+ selective plasma membrane (PM) channels, augmenting cytosolic Ca2+ levels and refilling ER stores. The principal molecular components of SOCE in many cell types include the ER-inserted stromal interaction molecule-1 (STIM1)3,4 and the Orai1 PM channel subunits5,6,7,8, which physically interact at ER???PM junctions in a functional Ca2+ release-activated Ca2+ (CRAC) channel complex. SOCE through CRAC channels is integral to myriad signalling pathways in electrically excitable and non-excitable cells, the most prominent being immune cells in which inheritable mutations in STIM1 and Orai1 have been linked to devastating immunodeficiency diseases. Therefore, we use this part to help with eliminating Ca2+ in hard water.
mutable notes: Usually, the protein Orai1 works only when the STIM1 channel is activated. However, we select the mutated protein that did not require the presistence of STIM1, which changed the 102th amino acid valine to alanine.
mutable provenance: This part comes from the cDNA of mutated Orai1 protein (V102A).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
sequence: https://synbiohub.org/public/igem/BBa_K2028000_sequence/1

BBa_K1614007
type: ComponentDefinition
persistent identity: BBa K1614007
display id: BBa_K1614007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: HRP-mimicking DNAzyme
creator: Frieda Anna Sorgenfrei
created: 2015-09-15T11:00:00Z
modified: 2015-09-16T06:57:25Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2031_
m user id: 12380
owner id: 24664
owning group id: 9
status: status/Planning
bookmark: true
mutable description: A DNAzyme with peroxidas acivity.
mutable provenance: Synthetic
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: HRP-mimicking DNAzyme
sequence: https://synbiohub.org/public/igem/BBa_K1614007_sequence/1

HRP-mimicking DNAzyme
type: SequenceAnnotation
persistent identity: annotation2458146
display id: annotation2458146
version: 1
top level: BBa_K1614007
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1614007/annotation2458146/range2458146/1

https://synbiohub.org/public/igem/BBa_K1614007/annotation2458146/range2458146/1
type: Range
persistent identity: range2458146
display id: range2458146
version: 1
direction: direction/forward
top level: BBa_K1614007
start: 1
end: 17
orientation: inline

O2
type: ComponentDefinition
persistent identity: BBa K2054002
display id: BBa_K2054002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Oligo 2 of DNA Tetrahedral Nanostructure
creator: LAI HEI WAI
created: 2016-10-13T11:00:00Z
modified: 2016-10-27T11:56:17Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2522_
m user id: 30316
owner id: 30316
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This will be used to build our desired nanostructure. This plasmid encodes the second ssDNA that will make the tetrahedral nanostructure, together with oligo 1, 3, 4 and 5.
mutable notes: The assembly of the structure.
mutable provenance: Elbaz, J., Yin, P., & Voigt, C. A. (2016). Genetic encoding of DNA nanostructures and their self-assembly in living bacteria. Nature communications, 7.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_K2054002_sequence/1

BBa_K1680015
type: ComponentDefinition
persistent identity: BBa K1680015
display id: BBa_K1680015
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pRS315 yeast shuttle vector with Biobrick MCS
creator: Katharina Sporbeck
created: 2015-09-16T11:00:00Z
modified: 2015-10-28T06:36:51Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2098_
m user id: 26366
owner id: 26460
owning group id: 9
status: status/Planning
bookmark: false
mutable description: A Shuttle vector for yeast. The vector is compatible with RFC10 standard and contains an ADH terminator behind the RFC10 cloning site. Ampicilin resistance for bakteria and URA3 gene for yeast selection.
mutable notes: contains an RFC10 compatible multiple cloning site, no restrictions sites for RFC10 in the Backbone. ADH Terminator after MCS.
mutable provenance: yeast shuttle vector
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: RFC10
sequence annotation: lac promotor
sequence annotation: CEN/ARS yeast centromere
sequence annotation: T3 promotor
sequence annotation: LEU2 promotor
sequence annotation: ampR promotor
sequence annotation: ampR
sequence annotation: f1 origin
sequence annotation: LEU2
sequence annotation: T7 promotor
sequence annotation: lacZalpha
sequence annotation: pUC origin
sequence: https://synbiohub.org/public/igem/BBa_K1680015_sequence/1

RFC10
type: SequenceAnnotation
persistent identity: annotation2466193
display id: annotation2466193
version: 1
top level: BBa_K1680015
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1680015/annotation2466193/range2466193/1

https://synbiohub.org/public/igem/BBa_K1680015/annotation2466193/range2466193/1
type: Range
persistent identity: range2466193
display id: range2466193
version: 1
direction: direction/forward
top level: BBa_K1680015
start: 3134
end: 3176
orientation: inline

lac promotor
type: SequenceAnnotation
persistent identity: annotation2466199
display id: annotation2466199
version: 1
top level: BBa_K1680015
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1680015/annotation2466199/range2466199/1

https://synbiohub.org/public/igem/BBa_K1680015/annotation2466199/range2466199/1
type: Range
persistent identity: range2466199
display id: range2466199
version: 1
direction: direction/reverse
top level: BBa_K1680015
start: 3292
end: 3322
orientation: inline

CEN/ARS yeast centromere
type: SequenceAnnotation
persistent identity: annotation2466204
display id: annotation2466204
version: 1
top level: BBa_K1680015
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1680015/annotation2466204/range2466204/1

https://synbiohub.org/public/igem/BBa_K1680015/annotation2466204/range2466204/1
type: Range
persistent identity: range2466204
display id: range2466204
version: 1
direction: direction/forward
top level: BBa_K1680015
start: 5407
end: 5910
orientation: inline

T3 promotor
type: SequenceAnnotation
persistent identity: annotation2466197
display id: annotation2466197
version: 1
top level: BBa_K1680015
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1680015/annotation2466197/range2466197/1

https://synbiohub.org/public/igem/BBa_K1680015/annotation2466197/range2466197/1
type: Range
persistent identity: range2466197
display id: range2466197
version: 1
direction: direction/reverse
top level: BBa_K1680015
start: 3205
end: 3223
orientation: inline

LEU2 promotor
type: SequenceAnnotation
persistent identity: annotation2466189
display id: annotation2466189
version: 1
top level: BBa_K1680015
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1680015/annotation2466189/range2466189/1

https://synbiohub.org/public/igem/BBa_K1680015/annotation2466189/range2466189/1
type: Range
persistent identity: range2466189
display id: range2466189
version: 1
direction: direction/reverse
top level: BBa_K1680015
start: 1770
end: 2174
orientation: inline

ampR promotor
type: SequenceAnnotation
persistent identity: annotation2466202
display id: annotation2466202
version: 1
top level: BBa_K1680015
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1680015/annotation2466202/range2466202/1

https://synbiohub.org/public/igem/BBa_K1680015/annotation2466202/range2466202/1
type: Range
persistent identity: range2466202
display id: range2466202
version: 1
direction: direction/reverse
top level: BBa_K1680015
start: 5266
end: 5370
orientation: inline

ampR
type: SequenceAnnotation
persistent identity: annotation2466201
display id: annotation2466201
version: 1
top level: BBa_K1680015
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1680015/annotation2466201/range2466201/1

https://synbiohub.org/public/igem/BBa_K1680015/annotation2466201/range2466201/1
type: Range
persistent identity: range2466201
display id: range2466201
version: 1
direction: direction/reverse
top level: BBa_K1680015
start: 4405
end: 5265
orientation: inline

f1 origin
type: SequenceAnnotation
persistent identity: annotation2466190
display id: annotation2466190
version: 1
top level: BBa_K1680015
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1680015/annotation2466190/range2466190/1

https://synbiohub.org/public/igem/BBa_K1680015/annotation2466190/range2466190/1
type: Range
persistent identity: range2466190
display id: range2466190
version: 1
direction: direction/reverse
top level: BBa_K1680015
start: 2474
end: 2929
orientation: inline

LEU2
type: SequenceAnnotation
persistent identity: annotation2466188
display id: annotation2466188
version: 1
top level: BBa_K1680015
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1680015/annotation2466188/range2466188/1

https://synbiohub.org/public/igem/BBa_K1680015/annotation2466188/range2466188/1
type: Range
persistent identity: range2466188
display id: range2466188
version: 1
direction: direction/reverse
top level: BBa_K1680015
start: 663
end: 1757
orientation: inline

T7 promotor
type: SequenceAnnotation
persistent identity: annotation2466192
display id: annotation2466192
version: 1
top level: BBa_K1680015
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1680015/annotation2466192/range2466192/1

https://synbiohub.org/public/igem/BBa_K1680015/annotation2466192/range2466192/1
type: Range
persistent identity: range2466192
display id: range2466192
version: 1
direction: direction/forward
top level: BBa_K1680015
start: 3097
end: 3115
orientation: inline

lacZalpha
type: SequenceAnnotation
persistent identity: annotation2466196
display id: annotation2466196
version: 1
top level: BBa_K1680015
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1680015/annotation2466196/range2466196/1

https://synbiohub.org/public/igem/BBa_K1680015/annotation2466196/range2466196/1
type: Range
persistent identity: range2466196
display id: range2466196
version: 1
direction: direction/reverse
top level: BBa_K1680015
start: 3186
end: 3248
orientation: inline

pUC origin
type: SequenceAnnotation
persistent identity: annotation2466200
display id: annotation2466200
version: 1
top level: BBa_K1680015
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1680015/annotation2466200/range2466200/1

https://synbiohub.org/public/igem/BBa_K1680015/annotation2466200/range2466200/1
type: Range
persistent identity: range2466200
display id: range2466200
version: 1
direction: direction/reverse
top level: BBa_K1680015
start: 3646
end: 4234
orientation: inline

lacZalpha
type: SequenceAnnotation
persistent identity: annotation2466191
display id: annotation2466191
version: 1
top level: BBa_K1680015
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1680015/annotation2466191/range2466191/1

https://synbiohub.org/public/igem/BBa_K1680015/annotation2466191/range2466191/1
type: Range
persistent identity: range2466191
display id: range2466191
version: 1
direction: direction/reverse
top level: BBa_K1680015
start: 2712
end: 3128
orientation: inline

IscA2
type: ComponentDefinition
persistent identity: BBa M36908
display id: BBa_M36908
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Coding Sequence of IscA2 pigeon homologue (codon-optimized for humans)
creator: Michael Becich
created: 2015-10-24T11:00:00Z
modified: 2015-12-04T08:06:13Z
discontinued: false
dominant: false
experience: experience/Fails
group u list: _848_
m user id: 29123
owner id: 29123
owning group id: 9
status: status/Planning
bookmark: false
mutable description: The gene Iron-Sulfur cluster assembly 2 (IscA2) is a mitochondrial protein involved in the biogenesis and assembly of iron-sulfur clusters, which play a role in electron-transfer reactions and is theorized to be associated with certain organisms' magnetic perception. The homing pigeon is one such organism with this ability, and here we have codon-optimized the Columba Livia homologue for homo sapiens. The sequence can be inserted into plasmids for expression of the magnetoreceptor IscA gene, which hopefully will create magnetogenetic biosensors in the form of iron-sulfur cluster, for downstream output and applications.
mutable notes: While the plasmid design for this insert can be complicated, the coding sequence itself is quite simple. We only needed to add the start codon since this second isoform did not have methionine built in.
mutable provenance: The predicted IscA amino acid sequence was obtained from NCBI (http://www.ncbi.nlm.nih.gov/protein/XP_005502393.1), and codon-optimized for humans with IDT's tools.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316

Tetrahedro
type: ComponentDefinition
persistent identity: BBa K2054000
display id: BBa_K2054000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: All Oligos for Tetrahedral Nanostructure
creator: LAI HEI WAI
created: 2016-10-13T11:00:00Z
modified: 2016-10-27T11:55:05Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2522_
m user id: 0
m user id: 30316
owner id: 30316
owning group id: 9
sample status: Not in stock
status: status/Planning
status: status/Unavailable
bookmark: false
mutable description: This will be used to build and express our desired functional nanostructure in E. coli that target micro RNA biomarkers. This plasmid encodes all the ssDNA that will make the tetrahedral nanostructure, i.e. Oligos 1, 2, 3, 4 and 5.
mutable notes: The assembly of the structure.
mutable provenance: Elbaz, J., Yin, P., & Voigt, C. A. (2016). Genetic encoding of DNA nanostructures and their self-assembly in living bacteria. Nature communications, 7.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_K2054000_sequence/1

O1
type: ComponentDefinition
persistent identity: BBa K2054001
display id: BBa_K2054001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Oligo 1
creator: LAI HEI WAI
created: 2016-10-13T11:00:00Z
modified: 2016-10-27T11:55:37Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2522_
m user id: 30316
owner id: 30316
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This will be use to build our desired nanostructure. This ssDNA contains also split G-quadruplex sequences that allow the oligo itself to function as an DNAzyme under specific conditions.
mutable notes: Strand Displacement
mutable provenance: Plasmid Construction: Elbaz, J., Yin, P., & Voigt, C. A. (2016). Genetic encoding of DNA nanostructures and their self-assembly in living bacteria. Nature communications, 7. Oligo Construction: Nakatsuka, K., Shigeto, H., Kuroda, A., & Funabashi, H. (2015). A split G-quadruplex-based DNA nano-tweezers structure as a signal-transducing molecule for the homogeneous detection of specific nucleic acids. Biosensors and Bioelectronics, 74, 222-226.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_K2054001_sequence/1

O5
type: ComponentDefinition
persistent identity: BBa K2054005
display id: BBa_K2054005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Oligo 5 of DNA Tetrahedral Nanostructure
creator: LAI HEI WAI
created: 2016-10-13T11:00:00Z
modified: 2016-10-27T11:57:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2522_
m user id: 0
m user id: 30316
owner id: 30316
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This will be used to build our desired nanostructure that target micro RNA biomarkers. This plasmid encodes the second ssDNA that will make the tetrahedral nanostructure, together with oligo 1, 2, 3 and 4.
mutable notes: The assembly of the structure.
mutable provenance: Elbaz, J., Yin, P., & Voigt, C. A. (2016). Genetic encoding of DNA nanostructures and their self-assembly in living bacteria. Nature communications, 7.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_K2054005_sequence/1

O4
type: ComponentDefinition
persistent identity: BBa K2054004
display id: BBa_K2054004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Oligo 4 of DNA Tetrahedral Nanostructure
creator: LAI HEI WAI
created: 2016-10-13T11:00:00Z
modified: 2016-10-27T11:57:02Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2522_
m user id: 0
m user id: 30316
owner id: 30316
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This will be used to build our desired nanostructure that target micro RNA biomarkers. This plasmid encodes the second ssDNA that will make the tetrahedral nanostructure, together with oligo 1, 2, 3 and 5.
mutable notes: The assembly of the structure.
mutable provenance: Elbaz, J., Yin, P., & Voigt, C. A. (2016). Genetic encoding of DNA nanostructures and their self-assembly in living bacteria. Nature communications, 7.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_K2054004_sequence/1

BBa_K1680005
type: ComponentDefinition
persistent identity: BBa K1680005
display id: BBa_K1680005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: loxP Site
creator: Katharina Sporbeck
created: 2015-09-16T11:00:00Z
modified: 2015-10-28T06:30:39Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2098_
m user id: 26366
owner id: 26460
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This part contains the WT loxP site, which is recognised by Cre recombinase (Part BBa_K1680007), in forward orientation.
mutable notes: Part is shipped in RFC10.
mutable provenance: gBlock designed by Team Tuebingen
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_K1680005_sequence/1

BBa_K1614013
type: ComponentDefinition
persistent identity: BBa K1614013
display id: BBa_K1614013
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: software generated kanamycin aptamer (candidate 2)
creator: Stefan Holderbach
created: 2015-09-15T11:00:00Z
modified: 2015-09-16T04:11:20Z
discontinued: false
dominant: false
group u list: _2031_
m user id: 12380
owner id: 12380
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Aptamer binding kanamycin created by the MAWS Software from iGEM Team Heidelberg
mutable notes: MAWS software from iGEM Team Heidelberg (created with the pre-alpha version)
mutable provenance: For further details on the in silico aptamer generation see:
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: Kanamycin aptamer
sequence: https://synbiohub.org/public/igem/BBa_K1614013_sequence/1

Kanamycin aptamer
type: SequenceAnnotation
persistent identity: annotation2458969
display id: annotation2458969
version: 1
top level: BBa_K1614013
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1614013/annotation2458969/range2458969/1

https://synbiohub.org/public/igem/BBa_K1614013/annotation2458969/range2458969/1
type: Range
persistent identity: range2458969
display id: range2458969
version: 1
direction: direction/forward
top level: BBa_K1614013
start: 1
end: 7
orientation: inline

BBa_K1614009
type: ComponentDefinition
persistent identity: BBa K1614009
display id: BBa_K1614009
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: software generated kanamycin aptamer (candidate 1)
creator: Stefan Holderbach
created: 2015-09-15T11:00:00Z
modified: 2015-09-16T03:19:18Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2031_
m user id: 12380
owner id: 12380
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Aptamer binding kanamycin created by the MAWS Software from iGEM Team Heidelberg
mutable notes: For further details on the in silico aptamer generation see:
mutable provenance: MAWS software from iGEM Team Heidelberg (created with the pre-alpha version)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: Kanamycin aptamer
sequence: https://synbiohub.org/public/igem/BBa_K1614009_sequence/1

Kanamycin aptamer
type: SequenceAnnotation
persistent identity: annotation2458458
display id: annotation2458458
version: 1
top level: BBa_K1614009
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1614009/annotation2458458/range2458458/1

https://synbiohub.org/public/igem/BBa_K1614009/annotation2458458/range2458458/1
type: Range
persistent identity: range2458458
display id: range2458458
version: 1
direction: direction/forward
top level: BBa_K1614009
start: 1
end: 7
orientation: inline

IscA1
type: ComponentDefinition
persistent identity: BBa M36907
display id: BBa_M36907
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Coding Sequence of IscA1 pigeon homologue (codon-optimized for humans)
creator: Michael Becich, Thomas Lau, Sreyas Misra
created: 2015-10-24T11:00:00Z
modified: 2015-12-04T07:45:15Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _848_
m user id: 29123
owner id: 29123
owning group id: 9
status: status/Planning
bookmark: true
mutable description: The gene Iron-Sulfur cluster assembly 1 (IscA1) is a mitochondrial protein involved in the biogenesis and assembly of iron-sulfur clusters, which play a role in electron-transfer reactions and is theorized to be associated with certain organisms' magnetic perception. The homing pigeon is one such organism with this ability, and here we have codon-optimized the Columba Livia homologue for homo sapiens. The sequence can be inserted into plasmids for expression of the magnetoreceptor IscA gene, which hopefully will create magnetogenetic biosensors in the form of iron-sulfur cluster, for downstream output and applications.
mutable notes: While the plasmid design for this insert can be complicated, the coding sequence itself is quite simple. We only needed to remove the start codon in order to co-express a fluorescent protein, and modify two BbsII restriction enzyme sites by altering the third base pair of codons (wobble effect) at bp locations 75 (C->G) and 309 (A->G).
mutable provenance: The predicted IscA amino acid sequence was obtained from NCBI (http://www.ncbi.nlm.nih.gov/protein/XP_005508102.1), and codon-optimized for humans with IDT's tools.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_M36907_sequence/1

MDA
type: ComponentDefinition
persistent identity: BBa M36761
display id: BBa_M36761
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Melamine deaminase coding sequence
creator: Ray Paul Laureano-Mercado & Mark Kirollos
created: 2013-06-09T11:00:00Z
modified: 2015-05-08T01:14:06Z
discontinued: false
dominant: false
experience: experience/None
group u list: _848_
m user id: 0
owner id: 17944
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This part is the coding sequence for the enzyme melamine deaminase, optimized for usage in E. coli. This enzyme eliminates an amine group from melamine to produce ammonia and ammeline and is able to remove another amine group at a rate 10 times slower than the first reaction to bring the compound to ammelide. The enzyme does not react to a measurable extent with this last compound, so it metabolizes melamine by consecutive and predictable deaminations.
mutable notes: The TriA gene???s codons were modified from the original and optimized for E. coli to a Codon Adaptation Index (CAI) of .76. After that, some palindromic and mRNA destabilizing sequences were removed to increase the stability of our DNA during synthesis.
mutable provenance: The original gene coding for the protein of interest was isolated from a species of Pseudomonas (http://www.ncbi.nlm.nih.gov/nuccore/11890744).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Melamine Deaminase
sequence annotation: Stop (TAG)
sequence annotation: Start (ATG)
sequence: https://synbiohub.org/public/igem/BBa_M36761_sequence/1

Melamine Deaminase
type: SequenceAnnotation
persistent identity: annotation2329380
display id: annotation2329380
version: 1
top level: MDA
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_M36761/annotation2329380/range2329380/1

https://synbiohub.org/public/igem/BBa_M36761/annotation2329380/range2329380/1
type: Range
persistent identity: range2329380
display id: range2329380
version: 1
direction: direction/forward
top level: MDA
start: 1
end: 1422
orientation: inline

Stop (TAG)
type: SequenceAnnotation
persistent identity: annotation2329382
display id: annotation2329382
version: 1
top level: MDA
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_M36761/annotation2329382/range2329382/1

https://synbiohub.org/public/igem/BBa_M36761/annotation2329382/range2329382/1
type: Range
persistent identity: range2329382
display id: range2329382
version: 1
direction: direction/forward
top level: MDA
start: 1423
end: 1425
orientation: inline

Start (ATG)
type: SequenceAnnotation
persistent identity: annotation2329381
display id: annotation2329381
version: 1
top level: MDA
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_M36761/annotation2329381/range2329381/1

https://synbiohub.org/public/igem/BBa_M36761/annotation2329381/range2329381/1
type: Range
persistent identity: range2329381
display id: range2329381
version: 1
direction: direction/forward
top level: MDA
start: 1
end: 3
orientation: inline

BBa_K1680016
type: ComponentDefinition
persistent identity: BBa K1680016
display id: BBa_K1680016
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pRS316 yeast shuttle vector with Biobrick MCS
creator: Katharina Sporbeck
created: 2015-09-16T11:00:00Z
modified: 2015-10-28T06:37:03Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2098_
m user id: 26366
owner id: 26460
owning group id: 9
status: status/Planning
bookmark: false
mutable description: A Shuttle vector for yeast. The vector is compatible with RFC10 standard and contains an ADH terminator behind the RFC10 cloning site. Ampicilin resistance for bacteria and URA3 gene for yeast selection.
mutable notes: Has an RFC10 compatible MCS, with ADH Terminator. No further RFC10 restrictions sites in the backbone.
mutable provenance: yeast shuttle vector
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: pUC origin
sequence annotation: lacZalpha
sequence annotation: f1 origin
sequence annotation: lac promotor
sequence annotation: URA3
sequence annotation: T3 promotor
sequence annotation: T7 promotor
sequence annotation: RFC10 MCS
sequence annotation: ampR
sequence annotation: URA3 promotor
sequence annotation: CEN/ARS yeast centromere
sequence annotation: ampR promotor
sequence: https://synbiohub.org/public/igem/BBa_K1680016_sequence/1

pUC origin
type: SequenceAnnotation
persistent identity: annotation2466222
display id: annotation2466222
version: 1
top level: BBa_K1680016
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1680016/annotation2466222/range2466222/1

https://synbiohub.org/public/igem/BBa_K1680016/annotation2466222/range2466222/1
type: Range
persistent identity: range2466222
display id: range2466222
version: 1
direction: direction/reverse
top level: BBa_K1680016
start: 2515
end: 3103
orientation: inline

lacZalpha
type: SequenceAnnotation
persistent identity: annotation2466217
display id: annotation2466217
version: 1
top level: BBa_K1680016
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1680016/annotation2466217/range2466217/1

https://synbiohub.org/public/igem/BBa_K1680016/annotation2466217/range2466217/1
type: Range
persistent identity: range2466217
display id: range2466217
version: 1
direction: direction/reverse
top level: BBa_K1680016
start: 2055
end: 2117
orientation: inline

f1 origin
type: SequenceAnnotation
persistent identity: annotation2466211
display id: annotation2466211
version: 1
top level: BBa_K1680016
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1680016/annotation2466211/range2466211/1

https://synbiohub.org/public/igem/BBa_K1680016/annotation2466211/range2466211/1
type: Range
persistent identity: range2466211
display id: range2466211
version: 1
direction: direction/reverse
top level: BBa_K1680016
start: 1343
end: 1798
orientation: inline

lacZalpha
type: SequenceAnnotation
persistent identity: annotation2466213
display id: annotation2466213
version: 1
top level: BBa_K1680016
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1680016/annotation2466213/range2466213/1

https://synbiohub.org/public/igem/BBa_K1680016/annotation2466213/range2466213/1
type: Range
persistent identity: range2466213
display id: range2466213
version: 1
direction: direction/reverse
top level: BBa_K1680016
start: 1581
end: 1997
orientation: inline

lac promotor
type: SequenceAnnotation
persistent identity: annotation2466221
display id: annotation2466221
version: 1
top level: BBa_K1680016
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1680016/annotation2466221/range2466221/1

https://synbiohub.org/public/igem/BBa_K1680016/annotation2466221/range2466221/1
type: Range
persistent identity: range2466221
display id: range2466221
version: 1
direction: direction/reverse
top level: BBa_K1680016
start: 2161
end: 2191
orientation: inline

URA3
type: SequenceAnnotation
persistent identity: annotation2466209
display id: annotation2466209
version: 1
top level: BBa_K1680016
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1680016/annotation2466209/range2466209/1

https://synbiohub.org/public/igem/BBa_K1680016/annotation2466209/range2466209/1
type: Range
persistent identity: range2466209
display id: range2466209
version: 1
direction: direction/forward
top level: BBa_K1680016
start: 411
end: 1214
orientation: inline

T3 promotor
type: SequenceAnnotation
persistent identity: annotation2466219
display id: annotation2466219
version: 1
top level: BBa_K1680016
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1680016/annotation2466219/range2466219/1

https://synbiohub.org/public/igem/BBa_K1680016/annotation2466219/range2466219/1
type: Range
persistent identity: range2466219
display id: range2466219
version: 1
direction: direction/reverse
top level: BBa_K1680016
start: 2073
end: 2092
orientation: inline

T7 promotor
type: SequenceAnnotation
persistent identity: annotation2466214
display id: annotation2466214
version: 1
top level: BBa_K1680016
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1680016/annotation2466214/range2466214/1

https://synbiohub.org/public/igem/BBa_K1680016/annotation2466214/range2466214/1
type: Range
persistent identity: range2466214
display id: range2466214
version: 1
direction: direction/forward
top level: BBa_K1680016
start: 1966
end: 1985
orientation: inline

RFC10 MCS
type: SequenceAnnotation
persistent identity: annotation2466215
display id: annotation2466215
version: 1
top level: BBa_K1680016
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1680016/annotation2466215/range2466215/1

https://synbiohub.org/public/igem/BBa_K1680016/annotation2466215/range2466215/1
type: Range
persistent identity: range2466215
display id: range2466215
version: 1
direction: direction/reverse
top level: BBa_K1680016
start: 2003
end: 2045
orientation: inline

ampR
type: SequenceAnnotation
persistent identity: annotation2466235
display id: annotation2466235
version: 1
top level: BBa_K1680016
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1680016/annotation2466235/range2466235/1

https://synbiohub.org/public/igem/BBa_K1680016/annotation2466235/range2466235/1
type: Range
persistent identity: range2466235
display id: range2466235
version: 1
direction: direction/reverse
top level: BBa_K1680016
start: 3274
end: 4134
orientation: inline

URA3 promotor
type: SequenceAnnotation
persistent identity: annotation2466206
display id: annotation2466206
version: 1
top level: BBa_K1680016
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1680016/annotation2466206/range2466206/1

https://synbiohub.org/public/igem/BBa_K1680016/annotation2466206/range2466206/1
type: Range
persistent identity: range2466206
display id: range2466206
version: 1
direction: direction/forward
top level: BBa_K1680016
start: 195
end: 410
orientation: inline

CEN/ARS yeast centromere
type: SequenceAnnotation
persistent identity: annotation2466237
display id: annotation2466237
version: 1
top level: BBa_K1680016
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1680016/annotation2466237/range2466237/1

https://synbiohub.org/public/igem/BBa_K1680016/annotation2466237/range2466237/1
type: Range
persistent identity: range2466237
display id: range2466237
version: 1
direction: direction/forward
top level: BBa_K1680016
start: 4276
end: 4779
orientation: inline

ampR promotor
type: SequenceAnnotation
persistent identity: annotation2466236
display id: annotation2466236
version: 1
top level: BBa_K1680016
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1680016/annotation2466236/range2466236/1

https://synbiohub.org/public/igem/BBa_K1680016/annotation2466236/range2466236/1
type: Range
persistent identity: range2466236
display id: range2466236
version: 1
direction: direction/reverse
top level: BBa_K1680016
start: 4135
end: 4239
orientation: inline

Active Com
type: ComponentDefinition
persistent identity: BBa K2054006
display id: BBa_K2054006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: All Oligos for simplified Tetrahedral Nanostructure
creator: LAI HEI WAI
created: 2016-10-13T11:00:00Z
modified: 2016-10-27T11:54:39Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2522_
m user id: 30316
owner id: 30316
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This will be used to build and express our desired functional nanostructure in E. coli that target micro RNA biomarkers, but a simplified 2-strand nanostructure. This plasmid encodes the two ssDNA that will make the nanostructure, i.e. Oligos 1 and 5, from parts BBa_K2054001 and BBa_K2054005 respectively.
mutable notes: The assembly of the structure.
mutable provenance: Elbaz, J., Yin, P., & Voigt, C. A. (2016). Genetic encoding of DNA nanostructures and their self-assembly in living bacteria. Nature communications, 7.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_K2054006_sequence/1