//direction/reverse
type: Collection
persistent identity: direction reverse
display id: direction_reverse
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
owned by: james
owned by: myers
member: BBa_J102001
member: BBa_B0020
member: BBa_K1486047
member: BBa_K137049
member: BBa_K137051
member: BBa_B0063
member: BBa_K093008
member: Plac(rev)
member: BBa_B0061
member: BBa_K1679040
member: BBa_K137050
member: BBa_K1486050
member: BBa_B0023
member: BBa_K137046
member: BBa_B0060
member: BBa_K110014
member: BBa_K1679026
member: BBa_B0022
member: BBa_K1679039
member: BBa_K137047
member: BBa_B0052
member: BBa_J44001
member: revRBS
member: BBa_K1679025
member: BBa_K110015
member: BBa_B0025
member: CmR
member: BBa_K137048

BBa_B0023
type: ComponentDefinition
persistent identity: BBa B0023
display id: BBa_B0023
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TE from coliphage T7, reversed
creator: Caitlin Conboy
created: 2003-10-16T11:00:00Z
modified: 2015-08-31T04:07:20Z
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This part is the reverse of terminator B0013, a bacteriophage T7 terminator which was engineered to be bidirectional. Contains a poly A insertion upstream of a 20 bp stem-loop.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence: https://synbiohub.org/public/igem/BBa_B0023_sequence/1

BBa_K093008
type: ComponentDefinition
persistent identity: BBa K093008
display id: BBa_K093008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: reverse BBa_R0011
creator: John Heil
created: 2008-10-27T12:00:00Z
modified: 2015-05-08T01:08:40Z
discontinued: false
dominant: false
experience: experience/None
group u list: _247_
m user id: 0
owner id: 3630
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: reverse compliments of BBa_R0011
mutable notes: for downstream application
mutable provenance: synthetic
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -10
sequence annotation: -35
sequence annotation: lacO1
sequence: https://synbiohub.org/public/igem/BBa_K093008_sequence/1

-10
type: SequenceAnnotation
persistent identity: annotation1992540
display id: annotation1992540
version: 1
top level: BBa_K093008
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K093008/annotation1992540/range1992540/1

https://synbiohub.org/public/igem/BBa_K093008/annotation1992540/range1992540/1
type: Range
persistent identity: range1992540
display id: range1992540
version: 1
direction: direction/reverse
top level: BBa_K093008
start: 8
end: 14
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1992541
display id: annotation1992541
version: 1
top level: BBa_K093008
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K093008/annotation1992541/range1992541/1

https://synbiohub.org/public/igem/BBa_K093008/annotation1992541/range1992541/1
type: Range
persistent identity: range1992541
display id: range1992541
version: 1
direction: direction/reverse
top level: BBa_K093008
start: 30
end: 35
orientation: inline

lacO1
type: SequenceAnnotation
persistent identity: annotation1992542
display id: annotation1992542
version: 1
top level: BBa_K093008
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K093008/annotation1992542/range1992542/1

https://synbiohub.org/public/igem/BBa_K093008/annotation1992542/range1992542/1
type: Range
persistent identity: range1992542
display id: range1992542
version: 1
direction: direction/reverse
top level: BBa_K093008
start: 36
end: 52
orientation: inline

lacO1
type: SequenceAnnotation
persistent identity: annotation1992543
display id: annotation1992543
version: 1
top level: BBa_K093008
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K093008/annotation1992543/range1992543/1

https://synbiohub.org/public/igem/BBa_K093008/annotation1992543/range1992543/1
type: Range
persistent identity: range1992543
display id: range1992543
version: 1
direction: direction/reverse
top level: BBa_K093008
start: 15
end: 29
orientation: inline

BBa_B0061
type: ComponentDefinition
persistent identity: BBa B0061
display id: BBa_B0061
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Terminator (Reverse B0051)
creator: Caitlin Conboy
created: 2004-01-29T12:00:00Z
modified: 2015-08-31T04:07:20Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Reverse of bi-directional terminator B0051. (E.coli derived, genbank U24206 bases 2694-2727. between converging yciA and tonA genes.)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0061_sequence/1

stem_loop
type: SequenceAnnotation
persistent identity: annotation318635
display id: annotation318635
version: 1
top level: BBa_B0061
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_B0061/annotation318635/range318635/1

https://synbiohub.org/public/igem/BBa_B0061/annotation318635/range318635/1
type: Range
persistent identity: range318635
display id: range318635
version: 1
direction: direction/forward
top level: BBa_B0061
start: 10
end: 25
orientation: inline

BBa_J44001
type: ComponentDefinition
persistent identity: BBa J44001
display id: BBa_J44001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Reverse RBS (RBSrev) -- corresponds to BBa_B0030
creator: Todd Eckdahl
created: 2006-08-01T11:00:00Z
modified: 2015-08-31T04:08:48Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _61_71_
m user id: 0
owner id: 606
owning group id: 61
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: Reverse version of RBS BBa_B0030
mutable notes: Repeats in oligos caused unusual products during cloning
mutable provenance: Cloned from synthetic oligos
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: Reverse RBS
sequence: https://synbiohub.org/public/igem/BBa_J44001_sequence/1

Reverse RBS
type: SequenceAnnotation
persistent identity: annotation1893199
display id: annotation1893199
version: 1
top level: BBa_J44001
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_J44001/annotation1893199/range1893199/1

https://synbiohub.org/public/igem/BBa_J44001/annotation1893199/range1893199/1
type: Range
persistent identity: range1893199
display id: range1893199
version: 1
direction: direction/reverse
top level: BBa_J44001
start: 1
end: 15
orientation: inline

BBa_K1679025
type: ComponentDefinition
persistent identity: BBa K1679025
display id: BBa_K1679025
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pT7-4 +RiboJ + GFP + LVA + 3 terminnators
creator: Xihan Zhang
created: 2015-09-16T11:00:00Z
modified: 2015-09-18T08:08:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2097_
m user id: 25072
owner id: 25072
owning group id: 9
status: status/Planning
bookmark: true
mutable description: This device is design for protein expression and promoter measurement. It constructed with a T7 RNA polymerase which has other 3 mutations, a RiboJ, a green fluorescent protein, a LVA degradation tag, a T7 terminator, a rrnB T1 terminator and a rrnB T2 terminator.
mutable notes: This device is design for protein expression and promoter measurement. It constructed with a T7 RNA polymerase which has other 3 mutations, a RiboJ, a green fluorescent protein, a LVA degradation tag, a T7 terminator, a rrnB T1 terminator and a rrnB T2 terminator. We added restriction sites flank these segments, so that users can change this segments into what they prefer. Users can replace T7 with other promoters through SalI and HindIII; replace RiboJ and GFP with other coding sequence through HindIII and StuI. After transcription, RiboJ becomes a section of functional RNA which comprises the sTRSV- ribozyme which can excise its upstream fragment to reduce noise, and an additional 23-nt hairpin immediately downstream to help expose the RBS. We use it to buffer synthetic circuits from genetic context. To adapt the protein expression device, three terminators are design to use cooperatively with strong promoters, the LVA degradation tag can accelerate protein degradation rate to decrease cell stress.
mutable provenance: Synthesized by Genewiz.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
sequence annotation: T7 terminator
sequence annotation: T7-4 promoter
sequence annotation: rrnB T1 terminator
sequence annotation: RiboJ
sequence annotation: GFP
sequence annotation: rbs
sequence annotation: LVA
sequence annotation: rrnB T2 terminator
sequence: https://synbiohub.org/public/igem/BBa_K1679025_sequence/1

T7 terminator
type: SequenceAnnotation
persistent identity: annotation2469892
display id: annotation2469892
version: 1
top level: BBa_K1679025
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1679025/annotation2469892/range2469892/1

https://synbiohub.org/public/igem/BBa_K1679025/annotation2469892/range2469892/1
type: Range
persistent identity: range2469892
display id: range2469892
version: 1
direction: direction/reverse
top level: BBa_K1679025
start: 223
end: 270
orientation: inline

T7-4 promoter
type: SequenceAnnotation
persistent identity: annotation2469897
display id: annotation2469897
version: 1
top level: BBa_K1679025
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1679025/annotation2469897/range2469897/1

https://synbiohub.org/public/igem/BBa_K1679025/annotation2469897/range2469897/1
type: Range
persistent identity: range2469897
display id: range2469897
version: 1
direction: direction/reverse
top level: BBa_K1679025
start: 1155
end: 1172
orientation: inline

rrnB T1 terminator
type: SequenceAnnotation
persistent identity: annotation2469891
display id: annotation2469891
version: 1
top level: BBa_K1679025
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1679025/annotation2469891/range2469891/1

https://synbiohub.org/public/igem/BBa_K1679025/annotation2469891/range2469891/1
type: Range
persistent identity: range2469891
display id: range2469891
version: 1
direction: direction/reverse
top level: BBa_K1679025
start: 128
end: 214
orientation: inline

RiboJ
type: SequenceAnnotation
persistent identity: annotation2469896
display id: annotation2469896
version: 1
top level: BBa_K1679025
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1679025/annotation2469896/range2469896/1

https://synbiohub.org/public/igem/BBa_K1679025/annotation2469896/range2469896/1
type: Range
persistent identity: range2469896
display id: range2469896
version: 1
direction: direction/reverse
top level: BBa_K1679025
start: 1074
end: 1148
orientation: inline

GFP
type: SequenceAnnotation
persistent identity: annotation2469894
display id: annotation2469894
version: 1
top level: BBa_K1679025
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1679025/annotation2469894/range2469894/1

https://synbiohub.org/public/igem/BBa_K1679025/annotation2469894/range2469894/1
type: Range
persistent identity: range2469894
display id: range2469894
version: 1
direction: direction/reverse
top level: BBa_K1679025
start: 335
end: 1048
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation2469895
display id: annotation2469895
version: 1
top level: BBa_K1679025
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1679025/annotation2469895/range2469895/1

https://synbiohub.org/public/igem/BBa_K1679025/annotation2469895/range2469895/1
type: Range
persistent identity: range2469895
display id: range2469895
version: 1
direction: direction/reverse
top level: BBa_K1679025
start: 1058
end: 1062
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation2469893
display id: annotation2469893
version: 1
top level: BBa_K1679025
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1679025/annotation2469893/range2469893/1

https://synbiohub.org/public/igem/BBa_K1679025/annotation2469893/range2469893/1
type: Range
persistent identity: range2469893
display id: range2469893
version: 1
direction: direction/reverse
top level: BBa_K1679025
start: 293
end: 334
orientation: inline

rrnB T2 terminator
type: SequenceAnnotation
persistent identity: annotation2469890
display id: annotation2469890
version: 1
top level: BBa_K1679025
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1679025/annotation2469890/range2469890/1

https://synbiohub.org/public/igem/BBa_K1679025/annotation2469890/range2469890/1
type: Range
persistent identity: range2469890
display id: range2469890
version: 1
direction: direction/reverse
top level: BBa_K1679025
start: 18
end: 45
orientation: inline

BBa_K137048
type: ComponentDefinition
persistent identity: BBa K137048
display id: BBa_K137048
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: 350 bp inverted tetR promoter
creator: Allen Lin
created: 2008-07-20T11:00:00Z
modified: 2015-05-08T01:10:09Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _187_
m user id: 0
owner id: 3112
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Inverted tetR promoter with noncoding, spacer DNA upstream of it to make the total length 350 bp.
mutable notes: This part is in a series of inverted tetR promoters that have total lengths of 150, 250, 350, 450, 650, and 850 bp. We chose the region upstream of tetR in part Q04400 to be the noncoding DNA segment. This noncoding segment consists of the 3' end of tetR and B0015. None of the parts in this series was long enough to include the start codon of tetR, so a functional tetR protein should not be transcribed.
mutable provenance: Primers were synthesized to bind to part Q04400, and then a PCR reaction was run.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: tetR promoter
sequence: https://synbiohub.org/public/igem/BBa_K137048_sequence/1

tetR promoter
type: SequenceAnnotation
persistent identity: annotation1968021
display id: annotation1968021
version: 1
top level: BBa_K137048
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K137048/annotation1968021/range1968021/1

https://synbiohub.org/public/igem/BBa_K137048/annotation1968021/range1968021/1
type: Range
persistent identity: range1968021
display id: range1968021
version: 1
direction: direction/reverse
top level: BBa_K137048
start: 1
end: 54
orientation: inline

BBa_B0052
type: ComponentDefinition
persistent identity: BBa B0052
display id: BBa_B0052
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Terminator (rrnC)
creator: Caitlin Conboy
created: 2004-01-29T12:00:00Z
modified: 2015-08-31T04:07:20Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Terminator from the E.coli ribosonal RNA rrnC operon. This terminator occurs in the reverse orientation upstream of the multiple cloning site of the pSB**1-pSB**3 series of plasmids.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0052_sequence/1

stem_loop
type: SequenceAnnotation
persistent identity: annotation318601
display id: annotation318601
version: 1
top level: BBa_B0052
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_B0052/annotation318601/range318601/1

https://synbiohub.org/public/igem/BBa_B0052/annotation318601/range318601/1
type: Range
persistent identity: range318601
display id: range318601
version: 1
direction: direction/forward
top level: BBa_B0052
start: 9
end: 28
orientation: inline

Plac(rev)
type: ComponentDefinition
persistent identity: BBa I742124
display id: BBa_I742124
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Reverse complement Lac promoter
creator: Caroline Dahl
created: 2007-10-21T11:00:00Z
modified: 2015-08-31T04:08:02Z
discontinued: false
dominant: false
experience: experience/None
group u list: _123_
m user id: 0
owner id: 1968
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This is an 'upside-down' version of I14032. It is a strong, constitual promoter. By adding this part, we wish to emphasise the possibility of engineering both DNA strands, and the additional reglatory possibility that this gives. The upstream sequence is non-functional and simply there to faciliate gel analysis or to physically enable the part to be recomineered (see the Edinburgh 2007 project where it is flanked by dif-sites).
mutable notes: When dealing with reverse complements of a locus, suffix and prefix need be fitted in opposite order.
mutable provenance: This sequence is part of the E.coli K12 genome.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: LacI binding site
sequence annotation: Plac -35
sequence annotation: CAP binding site
sequence annotation: Plac -10
sequence: https://synbiohub.org/public/igem/BBa_I742124_sequence/1

LacI binding site
type: SequenceAnnotation
persistent identity: annotation1955267
display id: annotation1955267
version: 1
top level: Plac(rev)
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_I742124/annotation1955267/range1955267/1

https://synbiohub.org/public/igem/BBa_I742124/annotation1955267/range1955267/1
type: Range
persistent identity: range1955267
display id: range1955267
version: 1
direction: direction/reverse
top level: Plac(rev)
start: 11
end: 31
orientation: inline

Plac -35
type: SequenceAnnotation
persistent identity: annotation1955298
display id: annotation1955298
version: 1
top level: Plac(rev)
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I742124/annotation1955298/range1955298/1

https://synbiohub.org/public/igem/BBa_I742124/annotation1955298/range1955298/1
type: Range
persistent identity: range1955298
display id: range1955298
version: 1
direction: direction/reverse
top level: Plac(rev)
start: 61
end: 66
orientation: inline

CAP binding site
type: SequenceAnnotation
persistent identity: annotation1955266
display id: annotation1955266
version: 1
top level: Plac(rev)
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_I742124/annotation1955266/range1955266/1

https://synbiohub.org/public/igem/BBa_I742124/annotation1955266/range1955266/1
type: Range
persistent identity: range1955266
display id: range1955266
version: 1
direction: direction/reverse
top level: Plac(rev)
start: 78
end: 115
orientation: inline

Plac -10
type: SequenceAnnotation
persistent identity: annotation1955299
display id: annotation1955299
version: 1
top level: Plac(rev)
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I742124/annotation1955299/range1955299/1

https://synbiohub.org/public/igem/BBa_I742124/annotation1955299/range1955299/1
type: Range
persistent identity: range1955299
display id: range1955299
version: 1
direction: direction/reverse
top level: Plac(rev)
start: 38
end: 43
orientation: inline

BBa_K1486047
type: ComponentDefinition
persistent identity: BBa K1486047
display id: BBa_K1486047
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CpxR promoter RV
creator: EPFL iGem team 2014
created: 2014-10-06T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: to edit
mutable notes: to edit
mutable provenance: to edit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K1486047_sequence/1

BBa_B0063
type: ComponentDefinition
persistent identity: BBa B0063
display id: BBa_B0063
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Terminator (Reverse B0053)
creator: Caitlin Conboy
created: 2004-01-29T12:00:00Z
modified: 2015-08-31T04:07:21Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Reverse of B0053, E.coli transcriptional terminator from the His operon.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0063_sequence/1

stem_loop
type: SequenceAnnotation
persistent identity: annotation318639
display id: annotation318639
version: 1
top level: BBa_B0063
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_B0063/annotation318639/range318639/1

https://synbiohub.org/public/igem/BBa_B0063/annotation318639/range318639/1
type: Range
persistent identity: range318639
display id: range318639
version: 1
direction: direction/forward
top level: BBa_B0063
start: 15
end: 64
orientation: inline

BBa_K137046
type: ComponentDefinition
persistent identity: BBa K137046
display id: BBa_K137046
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: 150 bp inverted tetR promoter
creator: Allen Lin
created: 2008-07-20T11:00:00Z
modified: 2015-05-08T01:10:09Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _187_
m user id: 0
owner id: 3112
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: tetR promoter with noncoding, spacer DNA upstream of it to make the total length 150 bp
mutable notes: This part is in a series of tetR promoter that have total lengths of 150, 250, 350, 450, 650, and 850 bp. We chose the region upstream of tetR in part Q04400 to be the noncoding DNA segment. This noncoding segment consists of the 3' end of tetR and B0015. None of the parts in this series was long enough to include the start codon of tetR, so a functional tetR protein should not be transcribed.
mutable provenance: Primers were synthesized to bind to part Q04400, and then a PCR reaction was run.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: tetR promoter
sequence: https://synbiohub.org/public/igem/BBa_K137046_sequence/1

tetR promoter
type: SequenceAnnotation
persistent identity: annotation1968019
display id: annotation1968019
version: 1
top level: BBa_K137046
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K137046/annotation1968019/range1968019/1

https://synbiohub.org/public/igem/BBa_K137046/annotation1968019/range1968019/1
type: Range
persistent identity: range1968019
display id: range1968019
version: 1
direction: direction/reverse
top level: BBa_K137046
start: 1
end: 54
orientation: inline

BBa_K137050
type: ComponentDefinition
persistent identity: BBa K137050
display id: BBa_K137050
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: 650 bp inverted tetR promoter
creator: Allen Lin
created: 2008-07-20T11:00:00Z
modified: 2015-05-08T01:10:09Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _187_
m user id: 0
owner id: 3112
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Inverted tetR promoter with noncoding, spacer DNA upstream of it to make the total length 650 bp.
mutable notes: This part is in a series of inverted tetR promoters that have total lengths of 150, 250, 350, 450, 650, and 850 bp. We chose the region upstream of tetR in part Q04400 to be the noncoding DNA segment. This noncoding segment consists of the 3' end of tetR and B0015. None of the parts in this series was long enough to include the start codon of tetR, so a functional tetR protein should not be transcribed.
mutable provenance: Primers were synthesized to bind to part Q04400, and then a PCR reaction was run.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: tetR promoter
sequence: https://synbiohub.org/public/igem/BBa_K137050_sequence/1

tetR promoter
type: SequenceAnnotation
persistent identity: annotation1968023
display id: annotation1968023
version: 1
top level: BBa_K137050
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K137050/annotation1968023/range1968023/1

https://synbiohub.org/public/igem/BBa_K137050/annotation1968023/range1968023/1
type: Range
persistent identity: range1968023
display id: range1968023
version: 1
direction: direction/reverse
top level: BBa_K137050
start: 1
end: 54
orientation: inline

BBa_K110014
type: ComponentDefinition
persistent identity: BBa K110014
display id: BBa_K110014
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: A-Cell Promoter MFA2 (backwards)
creator: James DiCarlo
created: 2008-08-26T11:00:00Z
modified: 2015-05-08T01:09:09Z
discontinued: false
dominant: false
experience: experience/None
group u list: _201_
m user id: 0
owner id: 2669
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is the same part as BBa_K110007 except it has Prefix and Suffix regions switched
mutable notes: This was taken from SGD
mutable provenance: SGD
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K110014_sequence/1

BBa_K137051
type: ComponentDefinition
persistent identity: BBa K137051
display id: BBa_K137051
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: 850 bp inverted tetR promoter
creator: Allen Lin
created: 2008-07-20T11:00:00Z
modified: 2015-05-08T01:10:09Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _187_
m user id: 0
owner id: 3112
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Inverted tetR promoter with noncoding, spacer DNA upstream of it to make the total length 650 bp.
mutable notes: This part is in a series of inverted tetR promoters that have total lengths of 150, 250, 350, 450, 650, and 850 bp. We chose the region upstream of tetR in part Q04400 to be the noncoding DNA segment. This noncoding segment consists of the 3' end of tetR and B0015. None of the parts in this series was long enough to include the start codon of tetR, so a functional tetR protein should not be transcribed.
mutable provenance: Primers were synthesized to bind to part Q04400, and then a PCR reaction was run.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: tetR promoter
sequence: https://synbiohub.org/public/igem/BBa_K137051_sequence/1

tetR promoter
type: SequenceAnnotation
persistent identity: annotation1968024
display id: annotation1968024
version: 1
top level: BBa_K137051
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K137051/annotation1968024/range1968024/1

https://synbiohub.org/public/igem/BBa_K137051/annotation1968024/range1968024/1
type: Range
persistent identity: range1968024
display id: range1968024
version: 1
direction: direction/reverse
top level: BBa_K137051
start: 1
end: 54
orientation: inline

BBa_J102001
type: ComponentDefinition
persistent identity: BBa J102001
display id: BBa_J102001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Reverse Lux Promoter
creator: Michael Pettigrew
created: 2010-07-01T11:00:00Z
modified: 2015-08-31T04:08:24Z
discontinued: false
dominant: false
group u list: _580_
m user id: 0
owner id: 5944
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This promoter is the right promoter in the lux cassette of Vibrio Fischeri. There is weak constitutive expression of downstream genes. Expression of downstream genes can be up-regulated by the action of the LuxR activator protein in complex with the autoinducer, 3-oxo-hexanoyl-HSL. Two molecules of LuxR protein can form a complex with two molecules of the AHL. This causes a conformational change in the protein, allowing it to bind to a site on the lux promoter and induce transcription.
mutable notes: None.
mutable provenance: This part was obtained by taking the reverse complement of R0062, ordering appropriate oligos, then annealing and extending them.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: promoter
sequence: https://synbiohub.org/public/igem/BBa_J102001_sequence/1

promoter
type: SequenceAnnotation
persistent identity: annotation2072374
display id: annotation2072374
version: 1
top level: BBa_J102001
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J102001/annotation2072374/range2072374/1

https://synbiohub.org/public/igem/BBa_J102001/annotation2072374/range2072374/1
type: Range
persistent identity: range2072374
display id: range2072374
version: 1
direction: direction/reverse
top level: BBa_J102001
start: 1
end: 55
orientation: inline

BBa_B0022
type: ComponentDefinition
persistent identity: BBa B0022
display id: BBa_B0022
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TE from coliphageT7, reversed
creator: Caitlin Conboy
created: 2003-10-16T11:00:00Z
modified: 2015-08-31T04:07:20Z
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Uni-directional terminator. Reverse of part B0012. 20 bp stem-loop.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence: https://synbiohub.org/public/igem/BBa_B0022_sequence/1

CmR
type: ComponentDefinition
persistent identity: BBa J61000
display id: BBa_J61000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: chloramphenicol resistance cassette
creator: John Anderson
created: 2006-07-30T11:00:00Z
modified: 2015-08-31T02:02:59Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Chloramphenicol resistance gene including its native promoter and ribosome binding site. Confers resistance to 25 ug/mL chloramphenicol. {pSB1A2-Bca1016}
mutable notes: N/A
mutable provenance: PCR ca1016F/R on pKD3 (934 bp, EcoRI/SpeI) Sub into pSB1A2-I13522 (EcoRI/SpeI) Products are Bca1015 and Bca1016 ---- ca1016F Forward EcoRI to biobrick CAT of pKD3 gacttgaattcgcggccgcttctagaggaataggaacttcatttaaatg ca1016R Reverse SpeI to biobrick CAT of pKD3 ccgctactagtggcgcgcctacctgtgacgg
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: CmR
sequence: https://synbiohub.org/public/igem/BBa_J61000_sequence/1

CmR
type: SequenceAnnotation
persistent identity: annotation1892270
display id: annotation1892270
version: 1
top level: CmR
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J61000/annotation1892270/range1892270/1

https://synbiohub.org/public/igem/BBa_J61000/annotation1892270/range1892270/1
type: Range
persistent identity: range1892270
display id: range1892270
version: 1
direction: direction/reverse
top level: CmR
start: 30
end: 687
orientation: inline

BBa_K1679039
type: ComponentDefinition
persistent identity: BBa K1679039
display id: BBa_K1679039
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pT7-10 + RiboJ + GFP + LVA + 3 terminnators
creator: Xihan Zhang
created: 2015-09-16T11:00:00Z
modified: 2015-09-17T09:03:49Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2097_
m user id: 25072
owner id: 25072
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This device is design for protein expression and promoter measurement. It constructed with a T7 RNA polymerase which has other 3 mutations, a RiboJ, a green fluorescent protein, a LVA degradation tag, a T7 terminator, a rrnB T1 terminator and a rrnB T2 terminator. The four T7 RNA polymerases are a family with different activity, the T7 RNA polymerase in this device have approximate relative activity 10%.
mutable notes: This device is design for protein expression and promoter measurement. It constructed with a T7 RNA polymerase which has other 3 mutations, a RiboJ, a green fluorescent protein, a LVA degradation tag, a T7 terminator, a rrnB T1 terminator and a rrnB T2 terminator. We added restriction sites flank these segments, so that users can change this segments into what they prefer. Users can replace T7 with other promoters through SalI and HindIII; replace RiboJ and GFP with other coding sequence through HindIII and StuI. After transcription, RiboJ becomes a section of functional RNA which comprises the sTRSV- ribozyme which can excise its upstream fragment to reduce noise, and an additional 23-nt hairpin immediately downstream to help expose the RBS. We use it to buffer synthetic circuits from genetic context. To adapt the protein expression device, three terminators are design to use cooperatively with strong promoters, the LVA degradation tag can accelerate protein degradation rate to decrease cell stress.
mutable provenance: Synthesized by Genewiz.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
sequence annotation: T7-10 promoter
sequence annotation: rrnB T1 terminator
sequence annotation: LVA
sequence annotation: RiboJ
sequence annotation: rrnB T2 terminator
sequence annotation: rbs
sequence annotation: GFP
sequence annotation: T7 terminator
sequence: https://synbiohub.org/public/igem/BBa_K1679039_sequence/1

T7-10 promoter
type: SequenceAnnotation
persistent identity: annotation2469914
display id: annotation2469914
version: 1
top level: BBa_K1679039
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1679039/annotation2469914/range2469914/1

https://synbiohub.org/public/igem/BBa_K1679039/annotation2469914/range2469914/1
type: Range
persistent identity: range2469914
display id: range2469914
version: 1
direction: direction/reverse
top level: BBa_K1679039
start: 1155
end: 1172
orientation: inline

rrnB T1 terminator
type: SequenceAnnotation
persistent identity: annotation2469908
display id: annotation2469908
version: 1
top level: BBa_K1679039
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1679039/annotation2469908/range2469908/1

https://synbiohub.org/public/igem/BBa_K1679039/annotation2469908/range2469908/1
type: Range
persistent identity: range2469908
display id: range2469908
version: 1
direction: direction/reverse
top level: BBa_K1679039
start: 128
end: 214
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation2469910
display id: annotation2469910
version: 1
top level: BBa_K1679039
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1679039/annotation2469910/range2469910/1

https://synbiohub.org/public/igem/BBa_K1679039/annotation2469910/range2469910/1
type: Range
persistent identity: range2469910
display id: range2469910
version: 1
direction: direction/reverse
top level: BBa_K1679039
start: 293
end: 334
orientation: inline

RiboJ
type: SequenceAnnotation
persistent identity: annotation2469913
display id: annotation2469913
version: 1
top level: BBa_K1679039
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1679039/annotation2469913/range2469913/1

https://synbiohub.org/public/igem/BBa_K1679039/annotation2469913/range2469913/1
type: Range
persistent identity: range2469913
display id: range2469913
version: 1
direction: direction/reverse
top level: BBa_K1679039
start: 1074
end: 1148
orientation: inline

rrnB T2 terminator
type: SequenceAnnotation
persistent identity: annotation2469907
display id: annotation2469907
version: 1
top level: BBa_K1679039
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1679039/annotation2469907/range2469907/1

https://synbiohub.org/public/igem/BBa_K1679039/annotation2469907/range2469907/1
type: Range
persistent identity: range2469907
display id: range2469907
version: 1
direction: direction/reverse
top level: BBa_K1679039
start: 18
end: 45
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation2469912
display id: annotation2469912
version: 1
top level: BBa_K1679039
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1679039/annotation2469912/range2469912/1

https://synbiohub.org/public/igem/BBa_K1679039/annotation2469912/range2469912/1
type: Range
persistent identity: range2469912
display id: range2469912
version: 1
direction: direction/reverse
top level: BBa_K1679039
start: 1058
end: 1062
orientation: inline

GFP
type: SequenceAnnotation
persistent identity: annotation2469911
display id: annotation2469911
version: 1
top level: BBa_K1679039
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1679039/annotation2469911/range2469911/1

https://synbiohub.org/public/igem/BBa_K1679039/annotation2469911/range2469911/1
type: Range
persistent identity: range2469911
display id: range2469911
version: 1
direction: direction/reverse
top level: BBa_K1679039
start: 335
end: 1048
orientation: inline

T7 terminator
type: SequenceAnnotation
persistent identity: annotation2469909
display id: annotation2469909
version: 1
top level: BBa_K1679039
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1679039/annotation2469909/range2469909/1

https://synbiohub.org/public/igem/BBa_K1679039/annotation2469909/range2469909/1
type: Range
persistent identity: range2469909
display id: range2469909
version: 1
direction: direction/reverse
top level: BBa_K1679039
start: 223
end: 270
orientation: inline

BBa_B0020
type: ComponentDefinition
persistent identity: BBa B0020
display id: BBa_B0020
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Terminator (Reverse B0010)
creator: Caitlin Conboy
created: 2003-10-07T11:00:00Z
modified: 2015-08-31T04:07:20Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop (B0010), cloned onto the antisense strand.
mutable provenance: Antiquity. thought to be T1 transcriptional terminator from E. coli RRNB operon.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0020_sequence/1

stem_loop
type: SequenceAnnotation
persistent identity: annotation354342
display id: annotation354342
version: 1
top level: BBa_B0020
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_B0020/annotation354342/range354342/1

https://synbiohub.org/public/igem/BBa_B0020/annotation354342/range354342/1
type: Range
persistent identity: range354342
display id: range354342
version: 1
direction: direction/forward
top level: BBa_B0020
start: 27
end: 70
orientation: inline

BBa_B0025
type: ComponentDefinition
persistent identity: BBa B0025
display id: BBa_B0025
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: double terminator (B0015), reversed
creator: Caitlin Conboy
created: 2003-12-02T12:00:00Z
modified: 2015-08-31T04:07:20Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: B0010
sequence annotation: B0012
sequence: https://synbiohub.org/public/igem/BBa_B0025_sequence/1

B0010
type: SequenceAnnotation
persistent identity: annotation369703
display id: annotation369703
version: 1
top level: BBa_B0025
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_B0025/annotation369703/range369703/1

https://synbiohub.org/public/igem/BBa_B0025/annotation369703/range369703/1
type: Range
persistent identity: range369703
display id: range369703
version: 1
direction: direction/reverse
top level: BBa_B0025
start: 50
end: 129
orientation: inline

B0012
type: SequenceAnnotation
persistent identity: annotation369702
display id: annotation369702
version: 1
top level: BBa_B0025
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_B0025/annotation369702/range369702/1

https://synbiohub.org/public/igem/BBa_B0025/annotation369702/range369702/1
type: Range
persistent identity: range369702
display id: range369702
version: 1
direction: direction/reverse
top level: BBa_B0025
start: 1
end: 41
orientation: inline

BBa_B0060
type: ComponentDefinition
persistent identity: BBa B0060
display id: BBa_B0060
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Terminator (Reverse B0050)
creator: Caitlin Conboy
created: 2004-01-29T12:00:00Z
modified: 2015-08-31T04:07:20Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Reverse Bi-directional terminator from B0050 (derived from pBR322).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0060_sequence/1

stem_loop
type: SequenceAnnotation
persistent identity: annotation318622
display id: annotation318622
version: 1
top level: BBa_B0060
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_B0060/annotation318622/range318622/1

https://synbiohub.org/public/igem/BBa_B0060/annotation318622/range318622/1
type: Range
persistent identity: range318622
display id: range318622
version: 1
direction: direction/forward
top level: BBa_B0060
start: 10
end: 25
orientation: inline

BBa_K110015
type: ComponentDefinition
persistent identity: BBa K110015
display id: BBa_K110015
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: A-Cell Promoter MFA1 (RtL)
creator: Rick Carrick, Ambhi Ganesan, Alex McMillan
created: 2008-08-26T11:00:00Z
modified: 2015-05-08T01:09:09Z
discontinued: false
dominant: false
experience: experience/None
group u list: _201_
m user id: 0
owner id: 2669
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This is the same sequence as MFA1 promotor(BBa_K110008), but with suffix and prefix switched
mutable notes: This is directlyh from the DNA sequence
mutable provenance: sgd
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: MATalpha2
sequence annotation: MCM1
sequence annotation: MFA1 promoter (RtL)
sequence annotation: STE12
sequence: https://synbiohub.org/public/igem/BBa_K110015_sequence/1

MATalpha2
type: SequenceAnnotation
persistent identity: annotation1981507
display id: annotation1981507
version: 1
top level: BBa_K110015
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K110015/annotation1981507/range1981507/1

https://synbiohub.org/public/igem/BBa_K110015/annotation1981507/range1981507/1
type: Range
persistent identity: range1981507
display id: range1981507
version: 1
direction: direction/forward
top level: BBa_K110015
start: 289
end: 297
orientation: inline

MATalpha2
type: SequenceAnnotation
persistent identity: annotation1981504
display id: annotation1981504
version: 1
top level: BBa_K110015
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K110015/annotation1981504/range1981504/1

https://synbiohub.org/public/igem/BBa_K110015/annotation1981504/range1981504/1
type: Range
persistent identity: range1981504
display id: range1981504
version: 1
direction: direction/forward
top level: BBa_K110015
start: 267
end: 276
orientation: inline

MCM1
type: SequenceAnnotation
persistent identity: annotation1981505
display id: annotation1981505
version: 1
top level: BBa_K110015
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K110015/annotation1981505/range1981505/1

https://synbiohub.org/public/igem/BBa_K110015/annotation1981505/range1981505/1
type: Range
persistent identity: range1981505
display id: range1981505
version: 1
direction: direction/forward
top level: BBa_K110015
start: 277
end: 287
orientation: inline

MFA1 promoter (RtL)
type: SequenceAnnotation
persistent identity: annotation1981503
display id: annotation1981503
version: 1
top level: BBa_K110015
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K110015/annotation1981503/range1981503/1

https://synbiohub.org/public/igem/BBa_K110015/annotation1981503/range1981503/1
type: Range
persistent identity: range1981503
display id: range1981503
version: 1
direction: direction/forward
top level: BBa_K110015
start: 1
end: 401
orientation: inline

STE12
type: SequenceAnnotation
persistent identity: annotation1981506
display id: annotation1981506
version: 1
top level: BBa_K110015
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K110015/annotation1981506/range1981506/1

https://synbiohub.org/public/igem/BBa_K110015/annotation1981506/range1981506/1
type: Range
persistent identity: range1981506
display id: range1981506
version: 1
direction: direction/forward
top level: BBa_K110015
start: 302
end: 309
orientation: inline

BBa_K1679026
type: ComponentDefinition
persistent identity: BBa K1679026
display id: BBa_K1679026
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pT7-21 + RiboJ + GFP + LVA + 3 terminnators
creator: Xihan Zhang
created: 2015-09-16T11:00:00Z
modified: 2015-09-18T08:09:28Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2097_
m user id: 25072
owner id: 25072
owning group id: 9
status: status/Planning
bookmark: true
mutable description: This device is design for protein expression and promoter measurement. It constructed with a T7 RNA polymerase which has other 3 mutations, a RiboJ, a green fluorescent protein, a LVA degradation tag, a T7 terminator, a rrnB T1 terminator and a rrnB T2 terminator. The four T7 RNA polymerases are a family with different activity, the T7 RNA polymerase in this device have approximate relative activity 21%.
mutable notes: This device is design for protein expression and promoter measurement. It constructed with a T7 RNA polymerase which has other 3 mutations, a RiboJ, a green fluorescent protein, a LVA degradation tag, a T7 terminator, a rrnB T1 terminator and a rrnB T2 terminator. We added restriction sites flank these segments, so that users can change this segments into what they prefer. Users can replace T7 with other promoters through SalI and HindIII; replace RiboJ and GFP with other coding sequence through HindIII and StuI. After transcription, RiboJ becomes a section of functional RNA which comprises the sTRSV- ribozyme which can excise its upstream fragment to reduce noise, and an additional 23-nt hairpin immediately downstream to help expose the RBS. We use it to buffer synthetic circuits from genetic context. To adapt the protein expression device, three terminators are design to use cooperatively with strong promoters, the LVA degradation tag can accelerate protein degradation rate to decrease cell stress.
mutable provenance: Synthesized by Genewize.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
sequence annotation: LVA
sequence annotation: T7-21 promoter
sequence annotation: T7 terminator
sequence annotation: rbs
sequence annotation: rrnB T1 terminator
sequence annotation: GFP
sequence annotation: rrnB T2 terminator
sequence annotation: RiboJ
sequence: https://synbiohub.org/public/igem/BBa_K1679026_sequence/1

LVA
type: SequenceAnnotation
persistent identity: annotation2469902
display id: annotation2469902
version: 1
top level: BBa_K1679026
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1679026/annotation2469902/range2469902/1

https://synbiohub.org/public/igem/BBa_K1679026/annotation2469902/range2469902/1
type: Range
persistent identity: range2469902
display id: range2469902
version: 1
direction: direction/reverse
top level: BBa_K1679026
start: 293
end: 334
orientation: inline

T7-21 promoter
type: SequenceAnnotation
persistent identity: annotation2469906
display id: annotation2469906
version: 1
top level: BBa_K1679026
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1679026/annotation2469906/range2469906/1

https://synbiohub.org/public/igem/BBa_K1679026/annotation2469906/range2469906/1
type: Range
persistent identity: range2469906
display id: range2469906
version: 1
direction: direction/reverse
top level: BBa_K1679026
start: 1155
end: 1172
orientation: inline

T7 terminator
type: SequenceAnnotation
persistent identity: annotation2469901
display id: annotation2469901
version: 1
top level: BBa_K1679026
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1679026/annotation2469901/range2469901/1

https://synbiohub.org/public/igem/BBa_K1679026/annotation2469901/range2469901/1
type: Range
persistent identity: range2469901
display id: range2469901
version: 1
direction: direction/reverse
top level: BBa_K1679026
start: 223
end: 270
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation2469904
display id: annotation2469904
version: 1
top level: BBa_K1679026
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1679026/annotation2469904/range2469904/1

https://synbiohub.org/public/igem/BBa_K1679026/annotation2469904/range2469904/1
type: Range
persistent identity: range2469904
display id: range2469904
version: 1
direction: direction/reverse
top level: BBa_K1679026
start: 1058
end: 1062
orientation: inline

rrnB T1 terminator
type: SequenceAnnotation
persistent identity: annotation2469900
display id: annotation2469900
version: 1
top level: BBa_K1679026
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1679026/annotation2469900/range2469900/1

https://synbiohub.org/public/igem/BBa_K1679026/annotation2469900/range2469900/1
type: Range
persistent identity: range2469900
display id: range2469900
version: 1
direction: direction/reverse
top level: BBa_K1679026
start: 128
end: 214
orientation: inline

GFP
type: SequenceAnnotation
persistent identity: annotation2469903
display id: annotation2469903
version: 1
top level: BBa_K1679026
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1679026/annotation2469903/range2469903/1

https://synbiohub.org/public/igem/BBa_K1679026/annotation2469903/range2469903/1
type: Range
persistent identity: range2469903
display id: range2469903
version: 1
direction: direction/reverse
top level: BBa_K1679026
start: 335
end: 1048
orientation: inline

rrnB T2 terminator
type: SequenceAnnotation
persistent identity: annotation2469899
display id: annotation2469899
version: 1
top level: BBa_K1679026
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1679026/annotation2469899/range2469899/1

https://synbiohub.org/public/igem/BBa_K1679026/annotation2469899/range2469899/1
type: Range
persistent identity: range2469899
display id: range2469899
version: 1
direction: direction/reverse
top level: BBa_K1679026
start: 18
end: 45
orientation: inline

RiboJ
type: SequenceAnnotation
persistent identity: annotation2469905
display id: annotation2469905
version: 1
top level: BBa_K1679026
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1679026/annotation2469905/range2469905/1

https://synbiohub.org/public/igem/BBa_K1679026/annotation2469905/range2469905/1
type: Range
persistent identity: range2469905
display id: range2469905
version: 1
direction: direction/reverse
top level: BBa_K1679026
start: 1074
end: 1148
orientation: inline

BBa_K137047
type: ComponentDefinition
persistent identity: BBa K137047
display id: BBa_K137047
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: 250 bp inverted tetR promoter
creator: Allen Lin
created: 2008-07-20T11:00:00Z
modified: 2015-05-08T01:10:09Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _187_
m user id: 0
owner id: 3112
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Inverted tetR promoter with noncoding, spacer DNA downstream of it to make the total length 250 bp. The promoter faces the 5' direction.
mutable notes: This part is in a series of inverted tetR promoters that have total lengths of 150, 250, 350, 450, 650, and 850 bp. We chose the region upstream of tetR in part Q04400 to be the noncoding DNA segment. This noncoding segment consists of the 3' end of tetR and B0015. None of the parts in this series was long enough to include the start codon of tetR, so a functional tetR protein should not be transcribed.
mutable provenance: Primers were synthesized to bind to part Q04400, and then a PCR reaction was run.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: tetR promoter
sequence: https://synbiohub.org/public/igem/BBa_K137047_sequence/1

tetR promoter
type: SequenceAnnotation
persistent identity: annotation1968020
display id: annotation1968020
version: 1
top level: BBa_K137047
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K137047/annotation1968020/range1968020/1

https://synbiohub.org/public/igem/BBa_K137047/annotation1968020/range1968020/1
type: Range
persistent identity: range1968020
display id: range1968020
version: 1
direction: direction/reverse
top level: BBa_K137047
start: 1
end: 54
orientation: inline

BBa_K137049
type: ComponentDefinition
persistent identity: BBa K137049
display id: BBa_K137049
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: 450 bp inverted tetR promoter
creator: Allen Lin
created: 2008-07-20T11:00:00Z
modified: 2015-05-08T01:10:09Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _187_
m user id: 0
owner id: 3112
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Inverted tetR promoter with noncoding, spacer DNA upstream of it to make the total length 450 bp.
mutable notes: This part is in a series of inverted tetR promoters that have total lengths of 150, 250, 350, 450, 650, and 850 bp. We chose the region upstream of tetR in part Q04400 to be the noncoding DNA segment. This noncoding segment consists of the 3' end of tetR and B0015. None of the parts in this series was long enough to include the start codon of tetR, so a functional tetR protein should not be transcribed.
mutable provenance: Primers were synthesized to bind to part Q04400, and then a PCR reaction was run.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: tetR promotoer
sequence: https://synbiohub.org/public/igem/BBa_K137049_sequence/1

tetR promotoer
type: SequenceAnnotation
persistent identity: annotation1968022
display id: annotation1968022
version: 1
top level: BBa_K137049
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K137049/annotation1968022/range1968022/1

https://synbiohub.org/public/igem/BBa_K137049/annotation1968022/range1968022/1
type: Range
persistent identity: range1968022
display id: range1968022
version: 1
direction: direction/reverse
top level: BBa_K137049
start: 1
end: 54
orientation: inline

BBa_K1486050
type: ComponentDefinition
persistent identity: BBa K1486050
display id: BBa_K1486050
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CpxR promoter RV + RFP
creator: EPFL iGem team 2014
created: 2014-10-06T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: to edit
mutable notes: to edit
mutable provenance: to edit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_K1486047
component: BBa_I13507
sequence annotation: BBa_I13507
sequence annotation: BBa_K1486047
sequence: https://synbiohub.org/public/igem/BBa_K1486050_sequence/1

BBa_K1486047
type: Component
persistent identity: component2398450
display id: component2398450
version: 1
top level: BBa_K1486050
access: public
definition: BBa_K1486047

BBa_I13507
type: Component
persistent identity: component2398462
display id: component2398462
version: 1
top level: BBa_K1486050
access: public
definition: https://synbiohub.org/public/igem/BBa_I13507/1

BBa_I13507
type: SequenceAnnotation
persistent identity: annotation2398462
display id: annotation2398462
version: 1
top level: BBa_K1486050
location: https://synbiohub.org/public/igem/BBa_K1486050/annotation2398462/range2398462/1
component: BBa_I13507

https://synbiohub.org/public/igem/BBa_K1486050/annotation2398462/range2398462/1
type: Range
persistent identity: range2398462
display id: range2398462
version: 1
direction: direction/reverse
top level: BBa_K1486050
start: 147
end: 1007
orientation: inline

BBa_K1486047
type: SequenceAnnotation
persistent identity: annotation2398450
display id: annotation2398450
version: 1
top level: BBa_K1486050
location: https://synbiohub.org/public/igem/BBa_K1486050/annotation2398450/range2398450/1
component: BBa_K1486047

https://synbiohub.org/public/igem/BBa_K1486050/annotation2398450/range2398450/1
type: Range
persistent identity: range2398450
display id: range2398450
version: 1
direction: direction/reverse
top level: BBa_K1486050
start: 1
end: 146
orientation: inline

revRBS
type: ComponentDefinition
persistent identity: BBa I742130
display id: BBa_I742130
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Reverse RBS
creator: Caroline Dahl
created: 2007-10-21T11:00:00Z
modified: 2015-08-31T04:08:03Z
discontinued: false
dominant: false
experience: experience/None
group u list: _123_
m user id: 0
owner id: 1968
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: DNA is double-stranded. To translate genes of both strands, you need RBSs in both strands.
mutable notes: Please refer to the original (BB_B0034).
mutable provenance: Please refer to the original (BB_B0034).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_I742130_sequence/1

BBa_K1679040
type: ComponentDefinition
persistent identity: BBa K1679040
display id: BBa_K1679040
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pT7-100 + RiboJ + GFP + LVA + 3 terminnators
creator: Xihan Zhang
created: 2015-09-16T11:00:00Z
modified: 2015-09-17T09:19:35Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2097_
m user id: 25072
owner id: 25072
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This device is design for protein expression and promoter measurement. It constructed with a T7 RNA polymerase which has other 3 mutations, a RiboJ, a green fluorescent protein, a LVA degradation tag, a T7 terminator, a rrnB T1 terminator and a rrnB T2 terminator. The four T7 RNA polymerases are a family with different activity, the T7 RNA polymerase in this device have approximate relative activity 100%.
mutable notes: This device is design for protein expression and promoter measurement. It constructed with a T7 RNA polymerase which has other 3 mutations, a RiboJ, a green fluorescent protein, a LVA degradation tag, a T7 terminator, a rrnB T1 terminator and a rrnB T2 terminator. We added restriction sites flank these segments, so that users can change this segments into what they prefer. Users can replace T7 with other promoters through SalI and HindIII; replace RiboJ and GFP with other coding sequence through HindIII and StuI. After transcription, RiboJ becomes a section of functional RNA which comprises the sTRSV- ribozyme which can excise its upstream fragment to reduce noise, and an additional 23-nt hairpin immediately downstream to help expose the RBS. We use it to buffer synthetic circuits from genetic context. To adapt the protein expression device, three terminators are design to use cooperatively with strong promoters, the LVA degradation tag can accelerate protein degradation rate to decrease cell stress.
mutable provenance: Mutated from BBa_k1679026 by PCR.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
sequence annotation: rrnB T1 terminator
sequence annotation: GFP
sequence annotation: T7-100 promoter
sequence annotation: LVA
sequence annotation: rbs
sequence annotation: RiboJ
sequence annotation: rrnB T2 terminator
sequence annotation: T7 terminator
sequence: https://synbiohub.org/public/igem/BBa_K1679040_sequence/1

rrnB T1 terminator
type: SequenceAnnotation
persistent identity: annotation2469916
display id: annotation2469916
version: 1
top level: BBa_K1679040
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1679040/annotation2469916/range2469916/1

https://synbiohub.org/public/igem/BBa_K1679040/annotation2469916/range2469916/1
type: Range
persistent identity: range2469916
display id: range2469916
version: 1
direction: direction/reverse
top level: BBa_K1679040
start: 128
end: 214
orientation: inline

GFP
type: SequenceAnnotation
persistent identity: annotation2469919
display id: annotation2469919
version: 1
top level: BBa_K1679040
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1679040/annotation2469919/range2469919/1

https://synbiohub.org/public/igem/BBa_K1679040/annotation2469919/range2469919/1
type: Range
persistent identity: range2469919
display id: range2469919
version: 1
direction: direction/reverse
top level: BBa_K1679040
start: 335
end: 1048
orientation: inline

T7-100 promoter
type: SequenceAnnotation
persistent identity: annotation2469922
display id: annotation2469922
version: 1
top level: BBa_K1679040
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1679040/annotation2469922/range2469922/1

https://synbiohub.org/public/igem/BBa_K1679040/annotation2469922/range2469922/1
type: Range
persistent identity: range2469922
display id: range2469922
version: 1
direction: direction/reverse
top level: BBa_K1679040
start: 1155
end: 1172
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation2469918
display id: annotation2469918
version: 1
top level: BBa_K1679040
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1679040/annotation2469918/range2469918/1

https://synbiohub.org/public/igem/BBa_K1679040/annotation2469918/range2469918/1
type: Range
persistent identity: range2469918
display id: range2469918
version: 1
direction: direction/reverse
top level: BBa_K1679040
start: 293
end: 334
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation2469920
display id: annotation2469920
version: 1
top level: BBa_K1679040
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1679040/annotation2469920/range2469920/1

https://synbiohub.org/public/igem/BBa_K1679040/annotation2469920/range2469920/1
type: Range
persistent identity: range2469920
display id: range2469920
version: 1
direction: direction/reverse
top level: BBa_K1679040
start: 1058
end: 1062
orientation: inline

RiboJ
type: SequenceAnnotation
persistent identity: annotation2469921
display id: annotation2469921
version: 1
top level: BBa_K1679040
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1679040/annotation2469921/range2469921/1

https://synbiohub.org/public/igem/BBa_K1679040/annotation2469921/range2469921/1
type: Range
persistent identity: range2469921
display id: range2469921
version: 1
direction: direction/reverse
top level: BBa_K1679040
start: 1074
end: 1148
orientation: inline

rrnB T2 terminator
type: SequenceAnnotation
persistent identity: annotation2469915
display id: annotation2469915
version: 1
top level: BBa_K1679040
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1679040/annotation2469915/range2469915/1

https://synbiohub.org/public/igem/BBa_K1679040/annotation2469915/range2469915/1
type: Range
persistent identity: range2469915
display id: range2469915
version: 1
direction: direction/reverse
top level: BBa_K1679040
start: 18
end: 45
orientation: inline

T7 terminator
type: SequenceAnnotation
persistent identity: annotation2469917
display id: annotation2469917
version: 1
top level: BBa_K1679040
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1679040/annotation2469917/range2469917/1

https://synbiohub.org/public/igem/BBa_K1679040/annotation2469917/range2469917/1
type: Range
persistent identity: range2469917
display id: range2469917
version: 1
direction: direction/reverse
top level: BBa_K1679040
start: 223
end: 270
orientation: inline