//collections/silk
type: Collection
persistent identity: collections silk
display id: collections_silk
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
owned by: james
owned by: myers
member: Tamura
member: BBa_K1763422
member: BBa_K1763430
member: BBa_K1085000
member: BBa_K844003
member: BBa_K1085005
member: BBa_K1085001
member: BBa_K1085040
member: BBa_K1085018
member: BBa_K1763003
member: PatgF1GFP
member: BBa_K1085010
member: BBa_K1317002
member: BBa_K1085006
member: BBa_K1085007
member: BBa_K1085002
member: BBa_K1085015
member: BBa_K1763015
member: BBa_K844001
member: PatgB4HT
member: BBa_K844008
member: BBa_K1085003
member: BBa_K1085024
member: BBa_K1085008
member: BBa_K1763423
member: BBa_K844017
member: BBa_K1085012
member: BBa_K1085029
member: BBa_K1162306

BBa_K1317002
type: ComponentDefinition
persistent identity: BBa K1317002
display id: BBa_K1317002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CDS of silk-like protein (SLP)
creator: Mounir Benkoulouche
created: 2014-10-06T11:00:00Z
modified: 2016-02-10T11:29:51Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _1692_
m user id: 4206
owner id: 20045
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part is the coding sequence for the silk like polypeptide. This sequence was assembled from a consensus of the MaSp1 from spiders. This protein, in spiders, allows resistance and elasticity used for spinning the webs. The synthetic gene encodes a synthetic protein "silk-like". The repeated aminoacids allow to retrieve these properties of resistance, resilience and elasticity, but with a minimal pattern of the original protein, which is more suited for downstream applications. It can be used to produce wire presenting these properties after wet-spinning.
mutable notes: The gene was synthesized by ordering specific oligo with overlapping regions, and by adding a NheI site for the assembling.
mutable provenance: major ampullate spidroin 1 (MaSp1) gene from spiders
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Start Codon
sequence annotation: ChewBack
sequence annotation: Aromatic (dosage)
sequence annotation: NheI
sequence annotation: Stop Codon
sequence annotation: CDS
sequence: https://synbiohub.org/public/igem/BBa_K1317002_sequence/1

Start Codon
type: SequenceAnnotation
persistent identity: annotation2398967
display id: annotation2398967
version: 1
top level: BBa_K1317002
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1317002/annotation2398967/range2398967/1

https://synbiohub.org/public/igem/BBa_K1317002/annotation2398967/range2398967/1
type: Range
persistent identity: range2398967
display id: range2398967
version: 1
direction: direction/forward
top level: BBa_K1317002
start: 6
end: 8
orientation: inline

ChewBack
type: SequenceAnnotation
persistent identity: annotation2398971
display id: annotation2398971
version: 1
top level: BBa_K1317002
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1317002/annotation2398971/range2398971/1

https://synbiohub.org/public/igem/BBa_K1317002/annotation2398971/range2398971/1
type: Range
persistent identity: range2398971
display id: range2398971
version: 1
direction: direction/forward
top level: BBa_K1317002
start: 318
end: 329
orientation: inline

Aromatic (dosage)
type: SequenceAnnotation
persistent identity: annotation2398970
display id: annotation2398970
version: 1
top level: BBa_K1317002
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1317002/annotation2398970/range2398970/1

https://synbiohub.org/public/igem/BBa_K1317002/annotation2398970/range2398970/1
type: Range
persistent identity: range2398970
display id: range2398970
version: 1
direction: direction/forward
top level: BBa_K1317002
start: 9
end: 11
orientation: inline

NheI
type: SequenceAnnotation
persistent identity: annotation2399216
display id: annotation2399216
version: 1
top level: BBa_K1317002
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1317002/annotation2399216/range2399216/1

https://synbiohub.org/public/igem/BBa_K1317002/annotation2399216/range2399216/1
type: Range
persistent identity: range2399216
display id: range2399216
version: 1
direction: direction/forward
top level: BBa_K1317002
start: 312
end: 317
orientation: inline

Stop Codon
type: SequenceAnnotation
persistent identity: annotation2398968
display id: annotation2398968
version: 1
top level: BBa_K1317002
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1317002/annotation2398968/range2398968/1

https://synbiohub.org/public/igem/BBa_K1317002/annotation2398968/range2398968/1
type: Range
persistent identity: range2398968
display id: range2398968
version: 1
direction: direction/forward
top level: BBa_K1317002
start: 330
end: 332
orientation: inline

CDS
type: SequenceAnnotation
persistent identity: annotation2398969
display id: annotation2398969
version: 1
top level: BBa_K1317002
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1317002/annotation2398969/range2398969/1

https://synbiohub.org/public/igem/BBa_K1317002/annotation2398969/range2398969/1
type: Range
persistent identity: range2398969
display id: range2398969
version: 1
direction: direction/forward
top level: BBa_K1317002
start: 6
end: 332
orientation: inline

BBa_K1085007
type: ComponentDefinition
persistent identity: BBa K1085007
display id: BBa_K1085007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: SpiderSilkSubunitN2 Strep-tag
creator: Claudio Tiecher
created: 2013-09-09T11:00:00Z
modified: 2016-02-10T01:06:40Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1395_
m user id: 0
m user id: 4206
owner id: 16089
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This BioBrick contains the coding sequence for part of the spider silk protein (SubunitN2) and a Strep-tag behind it. SubunitN2 codes for a spider silk protein that is optimized for maximal expression in Bacillus subtillus 168. The primary goal of the algorithm was to optimize the codon selection based on their availbility scores. The secondary goal was to minimize the number of restriction sites, and the tetrary goal was to prevent the formation of secondary RNA structures in close proximity to the RBS. The Strep-tag, fused at the C terminal, is used both as a binding tag to use to inidicate its production and as a binding component to biotin.
mutable notes: The DNA sequence was codon-optimized for Bacillus Subtilis. Moreover the nucleotide sequence was optimized in order to accomplish the production standard of IDT. A couple of optimized codons were affected by this.
mutable provenance: The amino acids sequence of the silk protein was obtained from MaSp2. The DNA sequence was synthesized by Integrated DNA Technologies (IDT).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: strep
sequence annotation: spidersilk subunit N2
sequence: https://synbiohub.org/public/igem/BBa_K1085007_sequence/1

strep
type: SequenceAnnotation
persistent identity: annotation2339057
display id: annotation2339057
version: 1
top level: BBa_K1085007
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1085007/annotation2339057/range2339057/1

https://synbiohub.org/public/igem/BBa_K1085007/annotation2339057/range2339057/1
type: Range
persistent identity: range2339057
display id: range2339057
version: 1
direction: direction/forward
top level: BBa_K1085007
start: 148
end: 171
orientation: inline

spidersilk subunit N2
type: SequenceAnnotation
persistent identity: annotation2339056
display id: annotation2339056
version: 1
top level: BBa_K1085007
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1085007/annotation2339056/range2339056/1

https://synbiohub.org/public/igem/BBa_K1085007/annotation2339056/range2339056/1
type: Range
persistent identity: range2339056
display id: range2339056
version: 1
direction: direction/forward
top level: BBa_K1085007
start: 1
end: 129
orientation: inline

BBa_K844003
type: ComponentDefinition
persistent identity: BBa K844003
display id: BBa_K844003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Spider Silk 1x Subunit "F" (Fewest tRNA codon optimized)
creator: Charles Barentine
created: 2012-10-01T11:00:00Z
modified: 2016-02-10T11:26:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1104_
m user id: 4206
owner id: 10131
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: Spider silk (1x) 2E subunit optimized to use the fewest number of tRNA codons; contains two elasticity domains and one strength domain.
mutable notes: none
mutable provenance: Artificially designed based on BBa_K844002 and DNA was synthesized.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Beta-spiral
sequence annotation: Beta-helix
sequence annotation: Strength Domain
sequence annotation: Elasticity Domain
sequence annotation: Linker Domain
sequence: https://synbiohub.org/public/igem/BBa_K844003_sequence/1

Beta-spiral
type: SequenceAnnotation
persistent identity: annotation2212261
display id: annotation2212261
version: 1
top level: BBa_K844003
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K844003/annotation2212261/range2212261/1

https://synbiohub.org/public/igem/BBa_K844003/annotation2212261/range2212261/1
type: Range
persistent identity: range2212261
display id: range2212261
version: 1
direction: direction/forward
top level: BBa_K844003
start: 46
end: 60
orientation: inline

Beta-spiral
type: SequenceAnnotation
persistent identity: annotation2212268
display id: annotation2212268
version: 1
top level: BBa_K844003
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K844003/annotation2212268/range2212268/1

https://synbiohub.org/public/igem/BBa_K844003/annotation2212268/range2212268/1
type: Range
persistent identity: range2212268
display id: range2212268
version: 1
direction: direction/forward
top level: BBa_K844003
start: 153
end: 168
orientation: inline

Beta-helix
type: SequenceAnnotation
persistent identity: annotation2212258
display id: annotation2212258
version: 1
top level: BBa_K844003
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K844003/annotation2212258/range2212258/1

https://synbiohub.org/public/igem/BBa_K844003/annotation2212258/range2212258/1
type: Range
persistent identity: range2212258
display id: range2212258
version: 1
direction: direction/forward
top level: BBa_K844003
start: 1
end: 9
orientation: inline

Strength Domain
type: SequenceAnnotation
persistent identity: annotation2212270
display id: annotation2212270
version: 1
top level: BBa_K844003
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K844003/annotation2212270/range2212270/1

https://synbiohub.org/public/igem/BBa_K844003/annotation2212270/range2212270/1
type: Range
persistent identity: range2212270
display id: range2212270
version: 1
direction: direction/forward
top level: BBa_K844003
start: 186
end: 204
orientation: inline

Beta-spiral
type: SequenceAnnotation
persistent identity: annotation2212259
display id: annotation2212259
version: 1
top level: BBa_K844003
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K844003/annotation2212259/range2212259/1

https://synbiohub.org/public/igem/BBa_K844003/annotation2212259/range2212259/1
type: Range
persistent identity: range2212259
display id: range2212259
version: 1
direction: direction/forward
top level: BBa_K844003
start: 10
end: 24
orientation: inline

Beta-spiral
type: SequenceAnnotation
persistent identity: annotation2212260
display id: annotation2212260
version: 1
top level: BBa_K844003
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K844003/annotation2212260/range2212260/1

https://synbiohub.org/public/igem/BBa_K844003/annotation2212260/range2212260/1
type: Range
persistent identity: range2212260
display id: range2212260
version: 1
direction: direction/forward
top level: BBa_K844003
start: 31
end: 45
orientation: inline

Elasticity Domain
type: SequenceAnnotation
persistent identity: annotation2212264
display id: annotation2212264
version: 1
top level: BBa_K844003
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K844003/annotation2212264/range2212264/1

https://synbiohub.org/public/igem/BBa_K844003/annotation2212264/range2212264/1
type: Range
persistent identity: range2212264
display id: range2212264
version: 1
direction: direction/forward
top level: BBa_K844003
start: 85
end: 168
orientation: inline

Elasticity Domain
type: SequenceAnnotation
persistent identity: annotation2212257
display id: annotation2212257
version: 1
top level: BBa_K844003
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K844003/annotation2212257/range2212257/1

https://synbiohub.org/public/igem/BBa_K844003/annotation2212257/range2212257/1
type: Range
persistent identity: range2212257
display id: range2212257
version: 1
direction: direction/forward
top level: BBa_K844003
start: 1
end: 84
orientation: inline

Beta-spiral
type: SequenceAnnotation
persistent identity: annotation2212266
display id: annotation2212266
version: 1
top level: BBa_K844003
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K844003/annotation2212266/range2212266/1

https://synbiohub.org/public/igem/BBa_K844003/annotation2212266/range2212266/1
type: Range
persistent identity: range2212266
display id: range2212266
version: 1
direction: direction/forward
top level: BBa_K844003
start: 114
end: 129
orientation: inline

Beta-spiral
type: SequenceAnnotation
persistent identity: annotation2212265
display id: annotation2212265
version: 1
top level: BBa_K844003
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K844003/annotation2212265/range2212265/1

https://synbiohub.org/public/igem/BBa_K844003/annotation2212265/range2212265/1
type: Range
persistent identity: range2212265
display id: range2212265
version: 1
direction: direction/forward
top level: BBa_K844003
start: 95
end: 110
orientation: inline

Linker Domain
type: SequenceAnnotation
persistent identity: annotation2212269
display id: annotation2212269
version: 1
top level: BBa_K844003
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K844003/annotation2212269/range2212269/1

https://synbiohub.org/public/igem/BBa_K844003/annotation2212269/range2212269/1
type: Range
persistent identity: range2212269
display id: range2212269
version: 1
direction: direction/forward
top level: BBa_K844003
start: 169
end: 185
orientation: inline

Beta-helix
type: SequenceAnnotation
persistent identity: annotation2212263
display id: annotation2212263
version: 1
top level: BBa_K844003
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K844003/annotation2212263/range2212263/1

https://synbiohub.org/public/igem/BBa_K844003/annotation2212263/range2212263/1
type: Range
persistent identity: range2212263
display id: range2212263
version: 1
direction: direction/forward
top level: BBa_K844003
start: 85
end: 94
orientation: inline

Beta-spiral
type: SequenceAnnotation
persistent identity: annotation2212262
display id: annotation2212262
version: 1
top level: BBa_K844003
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K844003/annotation2212262/range2212262/1

https://synbiohub.org/public/igem/BBa_K844003/annotation2212262/range2212262/1
type: Range
persistent identity: range2212262
display id: range2212262
version: 1
direction: direction/forward
top level: BBa_K844003
start: 69
end: 84
orientation: inline

Beta-spiral
type: SequenceAnnotation
persistent identity: annotation2212267
display id: annotation2212267
version: 1
top level: BBa_K844003
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K844003/annotation2212267/range2212267/1

https://synbiohub.org/public/igem/BBa_K844003/annotation2212267/range2212267/1
type: Range
persistent identity: range2212267
display id: range2212267
version: 1
direction: direction/forward
top level: BBa_K844003
start: 130
end: 144
orientation: inline

PatgF1GFP
type: ComponentDefinition
persistent identity: BBa K844015
display id: BBa_K844015
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: lac/IPTG inducible Spider Silk 1x "F" Subunit fused to GFP
creator: Brian Smith
created: 2012-10-01T11:00:00Z
modified: 2016-02-10T11:29:32Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1104_
m user id: 4206
owner id: 14072
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This part is a spider silk 1x "F" subunit with GFP fused to its C-terminus. This generator is lactose/ITPG inducible.
mutable notes: none
mutable provenance: Assembly standard #23 assembly of basic parts
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K208010
component: BBa_K844007
component: BBa_K208000
sequence annotation: BBa_K844007
sequence annotation: BBa_K208010
sequence annotation: BBa_K208000
sequence: https://synbiohub.org/public/igem/BBa_K844015_sequence/1

BBa_K208010
type: Component
persistent identity: component2211554
display id: component2211554
version: 1
top level: PatgF1GFP
access: public
definition: https://synbiohub.org/public/igem/BBa_K208010/1

BBa_K844007
type: Component
persistent identity: component2211555
display id: component2211555
version: 1
top level: PatgF1GFP
access: public
definition: https://synbiohub.org/public/igem/BBa_K844007/1

BBa_K208000
type: Component
persistent identity: component2211558
display id: component2211558
version: 1
top level: PatgF1GFP
access: public
definition: https://synbiohub.org/public/igem/BBa_K208000/1

BBa_K844007
type: SequenceAnnotation
persistent identity: annotation2211555
display id: annotation2211555
version: 1
top level: PatgF1GFP
location: https://synbiohub.org/public/igem/BBa_K844015/annotation2211555/range2211555/1
component: BBa_K844007

https://synbiohub.org/public/igem/BBa_K844015/annotation2211555/range2211555/1
type: Range
persistent identity: range2211555
display id: range2211555
version: 1
direction: direction/reverse
top level: PatgF1GFP
start: 227
end: 433
orientation: inline

BBa_K208010
type: SequenceAnnotation
persistent identity: annotation2211554
display id: annotation2211554
version: 1
top level: PatgF1GFP
location: https://synbiohub.org/public/igem/BBa_K844015/annotation2211554/range2211554/1
component: BBa_K208010

https://synbiohub.org/public/igem/BBa_K844015/annotation2211554/range2211554/1
type: Range
persistent identity: range2211554
display id: range2211554
version: 1
direction: direction/reverse
top level: PatgF1GFP
start: 1
end: 220
orientation: inline

BBa_K208000
type: SequenceAnnotation
persistent identity: annotation2211558
display id: annotation2211558
version: 1
top level: PatgF1GFP
location: https://synbiohub.org/public/igem/BBa_K844015/annotation2211558/range2211558/1
component: BBa_K208000

https://synbiohub.org/public/igem/BBa_K844015/annotation2211558/range2211558/1
type: Range
persistent identity: range2211558
display id: range2211558
version: 1
direction: direction/reverse
top level: PatgF1GFP
start: 440
end: 1159
orientation: inline

BBa_K1763423
type: ComponentDefinition
persistent identity: BBa K1763423
display id: BBa_K1763423
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: MaSp1-SeqAB2
creator: Vinson Lam
created: 2015-09-14T11:00:00Z
modified: 2016-02-10T11:27:38Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2185_
m user id: 4206
owner id: 27136
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This part contains the core sequence of MaSp2 that has been assembled with a 5'-AGTT-3' overhang on the 5' end of the sequence and 3'-ACAG-5' overhang on the 3' end of the sequence. This part was designed for use with ICA, along with parts BBa_K1763003 and BBa_K1763004 as an efficient method to assemble multiple MaSp2 sequences together. This setup is to show that this particular set of sticky ends is suitable for ICA. Before using in ICA, this part should be digested with BsaI, a type IIs restriction enzyme, which cuts DNA outside of its recognition site. Sequence and Features
mutable notes: The gene sequence for MaSp2 is particularly GC-rich, which may complicate PCR.
mutable provenance: This part is derived from the MaSp2 gene found in the spider N. Clavipes.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: B sticky end
sequence annotation: BsaI recognition site (forward)
sequence annotation: BsaI recognition site (reverse)
sequence annotation: MaSp1 SeqR Primer
sequence annotation: A sticky end
sequence annotation: MaSp1 Core Sequence
sequence annotation: MaSp1 SeqF Primer
sequence: https://synbiohub.org/public/igem/BBa_K1763423_sequence/1

B sticky end
type: SequenceAnnotation
persistent identity: annotation2463793
display id: annotation2463793
version: 1
top level: BBa_K1763423
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1763423/annotation2463793/range2463793/1

https://synbiohub.org/public/igem/BBa_K1763423/annotation2463793/range2463793/1
type: Range
persistent identity: range2463793
display id: range2463793
version: 1
direction: direction/forward
top level: BBa_K1763423
start: 109
end: 112
orientation: inline

BsaI recognition site (forward)
type: SequenceAnnotation
persistent identity: annotation2463789
display id: annotation2463789
version: 1
top level: BBa_K1763423
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1763423/annotation2463789/range2463789/1

https://synbiohub.org/public/igem/BBa_K1763423/annotation2463789/range2463789/1
type: Range
persistent identity: range2463789
display id: range2463789
version: 1
direction: direction/forward
top level: BBa_K1763423
start: 1
end: 5
orientation: inline

BsaI recognition site (reverse)
type: SequenceAnnotation
persistent identity: annotation2463790
display id: annotation2463790
version: 1
top level: BBa_K1763423
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1763423/annotation2463790/range2463790/1

https://synbiohub.org/public/igem/BBa_K1763423/annotation2463790/range2463790/1
type: Range
persistent identity: range2463790
display id: range2463790
version: 1
direction: direction/reverse
top level: BBa_K1763423
start: 114
end: 119
orientation: inline

MaSp1 SeqR Primer
type: SequenceAnnotation
persistent identity: annotation2473474
display id: annotation2473474
version: 1
top level: BBa_K1763423
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K1763423/annotation2473474/range2473474/1

https://synbiohub.org/public/igem/BBa_K1763423/annotation2473474/range2473474/1
type: Range
persistent identity: range2473474
display id: range2473474
version: 1
direction: direction/reverse
top level: BBa_K1763423
start: 41
end: 58
orientation: inline

A sticky end
type: SequenceAnnotation
persistent identity: annotation2463791
display id: annotation2463791
version: 1
top level: BBa_K1763423
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1763423/annotation2463791/range2463791/1

https://synbiohub.org/public/igem/BBa_K1763423/annotation2463791/range2463791/1
type: Range
persistent identity: range2463791
display id: range2463791
version: 1
direction: direction/forward
top level: BBa_K1763423
start: 7
end: 10
orientation: inline

MaSp1 Core Sequence
type: SequenceAnnotation
persistent identity: annotation2473472
display id: annotation2473472
version: 1
top level: BBa_K1763423
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1763423/annotation2473472/range2473472/1

https://synbiohub.org/public/igem/BBa_K1763423/annotation2473472/range2473472/1
type: Range
persistent identity: range2473472
display id: range2473472
version: 1
direction: direction/forward
top level: BBa_K1763423
start: 11
end: 108
orientation: inline

MaSp1 SeqF Primer
type: SequenceAnnotation
persistent identity: annotation2473473
display id: annotation2473473
version: 1
top level: BBa_K1763423
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K1763423/annotation2473473/range2473473/1

https://synbiohub.org/public/igem/BBa_K1763423/annotation2473473/range2473473/1
type: Range
persistent identity: range2473473
display id: range2473473
version: 1
direction: direction/forward
top level: BBa_K1763423
start: 41
end: 58
orientation: inline

BBa_K844001
type: ComponentDefinition
persistent identity: BBa K844001
display id: BBa_K844001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Spider Silk 1x 1E Subunit "U" (native sequence)
creator: Ryan Putman
created: 2012-10-01T11:00:00Z
modified: 2016-02-10T11:26:45Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1104_
m user id: 4206
owner id: 13965
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Spider silk subunit (1x) native sequence; contains one elasticity domain and one strength domain.
mutable notes: none
mutable provenance: Based on native 1E sequence from Brooks, et al. (2008) and DNA was artificially synthesized
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Beta-spiral
sequence annotation: Linker Domain
sequence annotation: Beta-Spiral
sequence annotation: Strength Domain
sequence annotation: Elasticity Domain
sequence annotation: Beta-helix
sequence: https://synbiohub.org/public/igem/BBa_K844001_sequence/1

Beta-spiral
type: SequenceAnnotation
persistent identity: annotation2212123
display id: annotation2212123
version: 1
top level: BBa_K844001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K844001/annotation2212123/range2212123/1

https://synbiohub.org/public/igem/BBa_K844001/annotation2212123/range2212123/1
type: Range
persistent identity: range2212123
display id: range2212123
version: 1
direction: direction/forward
top level: BBa_K844001
start: 10
end: 24
orientation: inline

Beta-spiral
type: SequenceAnnotation
persistent identity: annotation2212125
display id: annotation2212125
version: 1
top level: BBa_K844001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K844001/annotation2212125/range2212125/1

https://synbiohub.org/public/igem/BBa_K844001/annotation2212125/range2212125/1
type: Range
persistent identity: range2212125
display id: range2212125
version: 1
direction: direction/forward
top level: BBa_K844001
start: 46
end: 60
orientation: inline

Linker Domain
type: SequenceAnnotation
persistent identity: annotation2212127
display id: annotation2212127
version: 1
top level: BBa_K844001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K844001/annotation2212127/range2212127/1

https://synbiohub.org/public/igem/BBa_K844001/annotation2212127/range2212127/1
type: Range
persistent identity: range2212127
display id: range2212127
version: 1
direction: direction/forward
top level: BBa_K844001
start: 85
end: 102
orientation: inline

Beta-Spiral
type: SequenceAnnotation
persistent identity: annotation2212126
display id: annotation2212126
version: 1
top level: BBa_K844001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K844001/annotation2212126/range2212126/1

https://synbiohub.org/public/igem/BBa_K844001/annotation2212126/range2212126/1
type: Range
persistent identity: range2212126
display id: range2212126
version: 1
direction: direction/forward
top level: BBa_K844001
start: 69
end: 84
orientation: inline

Strength Domain
type: SequenceAnnotation
persistent identity: annotation2212128
display id: annotation2212128
version: 1
top level: BBa_K844001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K844001/annotation2212128/range2212128/1

https://synbiohub.org/public/igem/BBa_K844001/annotation2212128/range2212128/1
type: Range
persistent identity: range2212128
display id: range2212128
version: 1
direction: direction/forward
top level: BBa_K844001
start: 103
end: 120
orientation: inline

Elasticity Domain
type: SequenceAnnotation
persistent identity: annotation2212122
display id: annotation2212122
version: 1
top level: BBa_K844001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K844001/annotation2212122/range2212122/1

https://synbiohub.org/public/igem/BBa_K844001/annotation2212122/range2212122/1
type: Range
persistent identity: range2212122
display id: range2212122
version: 1
direction: direction/forward
top level: BBa_K844001
start: 1
end: 84
orientation: inline

Beta-spiral
type: SequenceAnnotation
persistent identity: annotation2212124
display id: annotation2212124
version: 1
top level: BBa_K844001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K844001/annotation2212124/range2212124/1

https://synbiohub.org/public/igem/BBa_K844001/annotation2212124/range2212124/1
type: Range
persistent identity: range2212124
display id: range2212124
version: 1
direction: direction/forward
top level: BBa_K844001
start: 31
end: 45
orientation: inline

Beta-helix
type: SequenceAnnotation
persistent identity: annotation2212121
display id: annotation2212121
version: 1
top level: BBa_K844001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K844001/annotation2212121/range2212121/1

https://synbiohub.org/public/igem/BBa_K844001/annotation2212121/range2212121/1
type: Range
persistent identity: range2212121
display id: range2212121
version: 1
direction: direction/forward
top level: BBa_K844001
start: 1
end: 9
orientation: inline

BBa_K1763430
type: ComponentDefinition
persistent identity: BBa K1763430
display id: BBa_K1763430
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: MaSp2-12(1C3)
creator: Vinson Lam
created: 2015-09-14T11:00:00Z
modified: 2016-02-10T11:30:09Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2185_
m user id: 4206
owner id: 27136
owning group id: 9
status: status/Planning
bookmark: false
mutable description: MaSp2 12 mer in pSB1C3. Does not contain regulatory elements
mutable notes: no
mutable provenance: N. clavipes
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: M2-AB
sequence annotation: M2-BC
sequence annotation: 8-his tag
sequence annotation: M2-CA
sequence: https://synbiohub.org/public/igem/BBa_K1763430_sequence/1

M2-AB
type: SequenceAnnotation
persistent identity: annotation2463869
display id: annotation2463869
version: 1
top level: BBa_K1763430
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1763430/annotation2463869/range2463869/1

https://synbiohub.org/public/igem/BBa_K1763430/annotation2463869/range2463869/1
type: Range
persistent identity: range2463869
display id: range2463869
version: 1
direction: direction/forward
top level: BBa_K1763430
start: 648
end: 749
orientation: inline

M2-BC
type: SequenceAnnotation
persistent identity: annotation2463868
display id: annotation2463868
version: 1
top level: BBa_K1763430
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1763430/annotation2463868/range2463868/1

https://synbiohub.org/public/igem/BBa_K1763430/annotation2463868/range2463868/1
type: Range
persistent identity: range2463868
display id: range2463868
version: 1
direction: direction/forward
top level: BBa_K1763430
start: 750
end: 851
orientation: inline

8-his tag
type: SequenceAnnotation
persistent identity: annotation2463861
display id: annotation2463861
version: 1
top level: BBa_K1763430
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1763430/annotation2463861/range2463861/1

https://synbiohub.org/public/igem/BBa_K1763430/annotation2463861/range2463861/1
type: Range
persistent identity: range2463861
display id: range2463861
version: 1
direction: direction/forward
top level: BBa_K1763430
start: 4
end: 27
orientation: inline

M2-BC
type: SequenceAnnotation
persistent identity: annotation2463865
display id: annotation2463865
version: 1
top level: BBa_K1763430
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1763430/annotation2463865/range2463865/1

https://synbiohub.org/public/igem/BBa_K1763430/annotation2463865/range2463865/1
type: Range
persistent identity: range2463865
display id: range2463865
version: 1
direction: direction/forward
top level: BBa_K1763430
start: 1056
end: 1157
orientation: inline

M2-BC
type: SequenceAnnotation
persistent identity: annotation2463867
display id: annotation2463867
version: 1
top level: BBa_K1763430
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1763430/annotation2463867/range2463867/1

https://synbiohub.org/public/igem/BBa_K1763430/annotation2463867/range2463867/1
type: Range
persistent identity: range2463867
display id: range2463867
version: 1
direction: direction/forward
top level: BBa_K1763430
start: 138
end: 239
orientation: inline

M2-CA
type: SequenceAnnotation
persistent identity: annotation2463863
display id: annotation2463863
version: 1
top level: BBa_K1763430
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1763430/annotation2463863/range2463863/1

https://synbiohub.org/public/igem/BBa_K1763430/annotation2463863/range2463863/1
type: Range
persistent identity: range2463863
display id: range2463863
version: 1
direction: direction/forward
top level: BBa_K1763430
start: 852
end: 953
orientation: inline

M2-AB
type: SequenceAnnotation
persistent identity: annotation2463873
display id: annotation2463873
version: 1
top level: BBa_K1763430
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1763430/annotation2463873/range2463873/1

https://synbiohub.org/public/igem/BBa_K1763430/annotation2463873/range2463873/1
type: Range
persistent identity: range2463873
display id: range2463873
version: 1
direction: direction/forward
top level: BBa_K1763430
start: 36
end: 137
orientation: inline

M2-AB
type: SequenceAnnotation
persistent identity: annotation2463866
display id: annotation2463866
version: 1
top level: BBa_K1763430
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1763430/annotation2463866/range2463866/1

https://synbiohub.org/public/igem/BBa_K1763430/annotation2463866/range2463866/1
type: Range
persistent identity: range2463866
display id: range2463866
version: 1
direction: direction/forward
top level: BBa_K1763430
start: 954
end: 1055
orientation: inline

M2-CA
type: SequenceAnnotation
persistent identity: annotation2463864
display id: annotation2463864
version: 1
top level: BBa_K1763430
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1763430/annotation2463864/range2463864/1

https://synbiohub.org/public/igem/BBa_K1763430/annotation2463864/range2463864/1
type: Range
persistent identity: range2463864
display id: range2463864
version: 1
direction: direction/forward
top level: BBa_K1763430
start: 1158
end: 1259
orientation: inline

M2-BC
type: SequenceAnnotation
persistent identity: annotation2463862
display id: annotation2463862
version: 1
top level: BBa_K1763430
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1763430/annotation2463862/range2463862/1

https://synbiohub.org/public/igem/BBa_K1763430/annotation2463862/range2463862/1
type: Range
persistent identity: range2463862
display id: range2463862
version: 1
direction: direction/forward
top level: BBa_K1763430
start: 444
end: 545
orientation: inline

M2-CA
type: SequenceAnnotation
persistent identity: annotation2463872
display id: annotation2463872
version: 1
top level: BBa_K1763430
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1763430/annotation2463872/range2463872/1

https://synbiohub.org/public/igem/BBa_K1763430/annotation2463872/range2463872/1
type: Range
persistent identity: range2463872
display id: range2463872
version: 1
direction: direction/forward
top level: BBa_K1763430
start: 546
end: 647
orientation: inline

M2-AB
type: SequenceAnnotation
persistent identity: annotation2463870
display id: annotation2463870
version: 1
top level: BBa_K1763430
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1763430/annotation2463870/range2463870/1

https://synbiohub.org/public/igem/BBa_K1763430/annotation2463870/range2463870/1
type: Range
persistent identity: range2463870
display id: range2463870
version: 1
direction: direction/forward
top level: BBa_K1763430
start: 342
end: 443
orientation: inline

M2-CA
type: SequenceAnnotation
persistent identity: annotation2463871
display id: annotation2463871
version: 1
top level: BBa_K1763430
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1763430/annotation2463871/range2463871/1

https://synbiohub.org/public/igem/BBa_K1763430/annotation2463871/range2463871/1
type: Range
persistent identity: range2463871
display id: range2463871
version: 1
direction: direction/forward
top level: BBa_K1763430
start: 240
end: 341
orientation: inline

BBa_K1085002
type: ComponentDefinition
persistent identity: BBa K1085002
display id: BBa_K1085002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: SpiderSilkSubunitE2
creator: Claudio Tiecher
created: 2013-09-09T11:00:00Z
modified: 2016-02-10T01:08:23Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1395_
m user id: 0
m user id: 4206
owner id: 16089
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This BioBrick contains the coding sequence for part of the spider silk protein (SubunitE1) with Bacillus Subtilis ribosome binding site (RBS) infront of it. The RBS is there to allow the ribosomes to bind and start to translate the DNA. SubunitE1 codes for a spider silk protein that is optimized for maximal expression in Bacillus subtillus 168. The primary goal of the algorithm was to optimize the codon selection based on their availbility scores. The secondary goal was to prevent the formation of secondary RNA structures in close proximity to the RBS, and the tetrary goal was to minimize the number of restriction sites. The amino acid sequence is based on the adapted MaSp2 sequences discribed in the paper of Brooks et. al. 2008.
mutable notes: The DNA sequence was codon-optimized for Bacillus Subtilis. Moreover the nucleotide sequence was optimized in order to accomplish the production standard of IDT. A couple of optimized codons were affected by this.
mutable provenance: The amino acids sequence of the silk protein was obtained from the paper by Brooks et al. (2008). The DNA sequence was synthesized by Integrated DNA Technologies (IDT).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: spidersilk subunit E2
sequence: https://synbiohub.org/public/igem/BBa_K1085002_sequence/1

spidersilk subunit E2
type: SequenceAnnotation
persistent identity: annotation2339043
display id: annotation2339043
version: 1
top level: BBa_K1085002
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1085002/annotation2339043/range2339043/1

https://synbiohub.org/public/igem/BBa_K1085002/annotation2339043/range2339043/1
type: Range
persistent identity: range2339043
display id: range2339043
version: 1
direction: direction/forward
top level: BBa_K1085002
start: 1
end: 210
orientation: inline

BBa_K1162306
type: ComponentDefinition
persistent identity: BBa K1162306
display id: BBa_K1162306
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Antimicrobial spider silk generator with C-terminus 10x His-Tag
creator: Ryan Putman
created: 2013-09-16T11:00:00Z
modified: 2016-02-10T11:27:24Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1474_
m user id: 4206
owner id: 13965
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: LL-37 + 8x spider silk
mutable notes: RFC 23. In frame fusion protein.
mutable provenance: Human (Homo sapiens) and Spider (Argiope aurantia)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K844004
component: BBa_K208010
component: BBa_K1162006
component: BBa_B0015
component: BBa_K844000
sequence annotation: BBa_K844004
sequence annotation: BBa_K1162006
sequence annotation: BBa_K208010
sequence annotation: BBa_K844000
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_K1162306_sequence/1

BBa_K844004
type: Component
persistent identity: component2351144
display id: component2351144
version: 1
top level: BBa_K1162306
access: public
definition: https://synbiohub.org/public/igem/BBa_K844004/1

BBa_K844004
type: Component
persistent identity: component2351129
display id: component2351129
version: 1
top level: BBa_K1162306
access: public
definition: https://synbiohub.org/public/igem/BBa_K844004/1

BBa_K208010
type: Component
persistent identity: component2351052
display id: component2351052
version: 1
top level: BBa_K1162306
access: public
definition: https://synbiohub.org/public/igem/BBa_K208010/1

BBa_K844004
type: Component
persistent identity: component2351159
display id: component2351159
version: 1
top level: BBa_K1162306
access: public
definition: https://synbiohub.org/public/igem/BBa_K844004/1

BBa_K844004
type: Component
persistent identity: component2351114
display id: component2351114
version: 1
top level: BBa_K1162306
access: public
definition: https://synbiohub.org/public/igem/BBa_K844004/1

BBa_K844004
type: Component
persistent identity: component2351099
display id: component2351099
version: 1
top level: BBa_K1162306
access: public
definition: https://synbiohub.org/public/igem/BBa_K844004/1

BBa_K1162006
type: Component
persistent identity: component2351054
display id: component2351054
version: 1
top level: BBa_K1162306
access: public
definition: https://synbiohub.org/public/igem/BBa_K1162006/1

BBa_K844004
type: Component
persistent identity: component2351174
display id: component2351174
version: 1
top level: BBa_K1162306
access: public
definition: https://synbiohub.org/public/igem/BBa_K844004/1

BBa_B0015
type: Component
persistent identity: component2351185
display id: component2351185
version: 1
top level: BBa_K1162306
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_K844004
type: Component
persistent identity: component2351084
display id: component2351084
version: 1
top level: BBa_K1162306
access: public
definition: https://synbiohub.org/public/igem/BBa_K844004/1

BBa_K844000
type: Component
persistent identity: component2351178
display id: component2351178
version: 1
top level: BBa_K1162306
access: public
definition: https://synbiohub.org/public/igem/BBa_K844000/1

BBa_K844004
type: Component
persistent identity: component2351069
display id: component2351069
version: 1
top level: BBa_K1162306
access: public
definition: https://synbiohub.org/public/igem/BBa_K844004/1

BBa_K844004
type: SequenceAnnotation
persistent identity: annotation2351114
display id: annotation2351114
version: 1
top level: BBa_K1162306
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display id: range2351114
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start: 985
end: 1188
orientation: inline

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type: SequenceAnnotation
persistent identity: annotation2351054
display id: annotation2351054
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direction: direction/reverse
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end: 340
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BBa_K844004
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display id: annotation2351159
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end: 1824
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BBa_K208010
type: SequenceAnnotation
persistent identity: annotation2351052
display id: annotation2351052
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location: https://synbiohub.org/public/igem/BBa_K1162306/annotation2351052/range2351052/1
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persistent identity: range2351069
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BBa_K844004
type: SequenceAnnotation
persistent identity: annotation2351099
display id: annotation2351099
version: 1
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location: https://synbiohub.org/public/igem/BBa_K1162306/annotation2351099/range2351099/1
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BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2351185
display id: annotation2351185
version: 1
top level: BBa_K1162306
location: https://synbiohub.org/public/igem/BBa_K1162306/annotation2351185/range2351185/1
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end: 2217
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BBa_K844004
type: SequenceAnnotation
persistent identity: annotation2351084
display id: annotation2351084
version: 1
top level: BBa_K1162306
location: https://synbiohub.org/public/igem/BBa_K1162306/annotation2351084/range2351084/1
component: BBa_K844004

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type: Range
persistent identity: range2351084
display id: range2351084
version: 1
direction: direction/reverse
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start: 561
end: 764
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BBa_K844017
type: ComponentDefinition
persistent identity: BBa K844017
display id: BBa_K844017
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Spider Silk Generator - 10x "F" Silk Construct with His Tag
creator: Ryan Putman
created: 2012-10-24T11:00:00Z
modified: 2016-02-10T11:29:22Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1104_
m user id: 4206
owner id: 6788
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Description Goes Here
mutable notes: none
mutable provenance: Created with standard assembly (standard #23)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K844003
component: BBa_K844000
component: BBa_K844007
component: BBa_K208010
sequence annotation: BBa_K208010
sequence annotation: BBa_K844007
sequence annotation: BBa_K844003
sequence annotation: BBa_K844000
sequence: https://synbiohub.org/public/igem/BBa_K844017_sequence/1

BBa_K844003
type: Component
persistent identity: component2212647
display id: component2212647
version: 1
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access: public
definition: BBa_K844003

BBa_K844003
type: Component
persistent identity: component2212662
display id: component2212662
version: 1
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access: public
definition: BBa_K844003

BBa_K844000
type: Component
persistent identity: component2212771
display id: component2212771
version: 1
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access: public
definition: https://synbiohub.org/public/igem/BBa_K844000/1

BBa_K844003
type: Component
persistent identity: component2212752
display id: component2212752
version: 1
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access: public
definition: BBa_K844003

BBa_K844003
type: Component
persistent identity: component2212692
display id: component2212692
version: 1
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access: public
definition: BBa_K844003

BBa_K844003
type: Component
persistent identity: component2212767
display id: component2212767
version: 1
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access: public
definition: BBa_K844003

BBa_K844003
type: Component
persistent identity: component2212677
display id: component2212677
version: 1
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definition: BBa_K844003

BBa_K844007
type: Component
persistent identity: component2212632
display id: component2212632
version: 1
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access: public
definition: https://synbiohub.org/public/igem/BBa_K844007/1

BBa_K844003
type: Component
persistent identity: component2212737
display id: component2212737
version: 1
top level: BBa_K844017
access: public
definition: BBa_K844003

BBa_K844003
type: Component
persistent identity: component2212722
display id: component2212722
version: 1
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access: public
definition: BBa_K844003

BBa_K208010
type: Component
persistent identity: component2212616
display id: component2212616
version: 1
top level: BBa_K844017
access: public
definition: https://synbiohub.org/public/igem/BBa_K208010/1

BBa_K844003
type: Component
persistent identity: component2212707
display id: component2212707
version: 1
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access: public
definition: BBa_K844003

BBa_K208010
type: SequenceAnnotation
persistent identity: annotation2212616
display id: annotation2212616
version: 1
top level: BBa_K844017
location: https://synbiohub.org/public/igem/BBa_K844017/annotation2212616/range2212616/1
component: BBa_K208010

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version: 1
direction: direction/reverse
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start: 1
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orientation: inline

BBa_K844007
type: SequenceAnnotation
persistent identity: annotation2212632
display id: annotation2212632
version: 1
top level: BBa_K844017
location: https://synbiohub.org/public/igem/BBa_K844017/annotation2212632/range2212632/1
component: BBa_K844007

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type: Range
persistent identity: range2212632
display id: range2212632
version: 1
direction: direction/reverse
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start: 227
end: 433
orientation: inline

BBa_K844003
type: SequenceAnnotation
persistent identity: annotation2212752
display id: annotation2212752
version: 1
top level: BBa_K844017
location: https://synbiohub.org/public/igem/BBa_K844017/annotation2212752/range2212752/1
component: BBa_K844003

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persistent identity: range2212752
display id: range2212752
version: 1
direction: direction/reverse
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start: 1926
end: 2129
orientation: inline

BBa_K844003
type: SequenceAnnotation
persistent identity: annotation2212722
display id: annotation2212722
version: 1
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persistent identity: range2212722
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version: 1
direction: direction/reverse
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end: 1705
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BBa_K844003
type: SequenceAnnotation
persistent identity: annotation2212707
display id: annotation2212707
version: 1
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persistent identity: range2212707
display id: range2212707
version: 1
direction: direction/reverse
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BBa_K844003
type: SequenceAnnotation
persistent identity: annotation2212692
display id: annotation2212692
version: 1
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direction: direction/reverse
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end: 1281
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BBa_K844000
type: SequenceAnnotation
persistent identity: annotation2212771
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version: 1
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BBa_K844003
type: SequenceAnnotation
persistent identity: annotation2212662
display id: annotation2212662
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component: BBa_K844003

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persistent identity: range2212662
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version: 1
direction: direction/reverse
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BBa_K844003
type: SequenceAnnotation
persistent identity: annotation2212767
display id: annotation2212767
version: 1
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component: BBa_K844003

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version: 1
direction: direction/reverse
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start: 2138
end: 2341
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type: SequenceAnnotation
persistent identity: annotation2212737
display id: annotation2212737
version: 1
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component: BBa_K844003

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persistent identity: range2212737
display id: range2212737
version: 1
direction: direction/reverse
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start: 1714
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type: SequenceAnnotation
persistent identity: annotation2212677
display id: annotation2212677
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component: BBa_K844003

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type: Range
persistent identity: range2212677
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version: 1
direction: direction/reverse
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start: 866
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BBa_K844003
type: SequenceAnnotation
persistent identity: annotation2212647
display id: annotation2212647
version: 1
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location: https://synbiohub.org/public/igem/BBa_K844017/annotation2212647/range2212647/1
component: BBa_K844003

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type: Range
persistent identity: range2212647
display id: range2212647
version: 1
direction: direction/reverse
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BBa_K1763003
type: ComponentDefinition
persistent identity: BBa K1763003
display id: BBa_K1763003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Major Spidroin Protein 2 (MaSp2) with sticky ends BC
creator: Vinson Lam
created: 2015-06-01T11:00:00Z
modified: 2016-02-10T11:34:10Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2185_
m user id: 4206
owner id: 27136
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This part contains the core sequence of MaSp2 that has been assembled with a 5'-TGTC-3' overhang on the 5' end of the sequence and 3'-GCAC-5' overhang on the 3' end of the sequence. This part was designed for use with ICA, along with parts BBa_K1763003 and BBa_K1763004 as an efficient method to assemble multiple MaSp2 sequences together. This setup is to show that this particular set of sticky ends is suitable for ICA. Before using in ICA, this part should be digested with BsaI, a type IIs restriction enzyme, which cuts DNA outside of its recognition site.
mutable notes: The gene sequence for MaSp2 is particularly GC-rich, which may complicate PCR.
mutable provenance: This part is derived from the MaSp2 gene found in the spider N. Clavipes.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: B sticky end
sequence annotation: C sticky end
sequence annotation: BsaI recognition site (forward)
sequence annotation: MaSp2 Core Sequence
sequence annotation: BsaI reconition site (reverse)
sequence: https://synbiohub.org/public/igem/BBa_K1763003_sequence/1

B sticky end
type: SequenceAnnotation
persistent identity: annotation2463759
display id: annotation2463759
version: 1
top level: BBa_K1763003
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1763003/annotation2463759/range2463759/1

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type: Range
persistent identity: range2463759
display id: range2463759
version: 1
direction: direction/forward
top level: BBa_K1763003
start: 7
end: 10
orientation: inline

C sticky end
type: SequenceAnnotation
persistent identity: annotation2463760
display id: annotation2463760
version: 1
top level: BBa_K1763003
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1763003/annotation2463760/range2463760/1

https://synbiohub.org/public/igem/BBa_K1763003/annotation2463760/range2463760/1
type: Range
persistent identity: range2463760
display id: range2463760
version: 1
direction: direction/forward
top level: BBa_K1763003
start: 109
end: 112
orientation: inline

BsaI recognition site (forward)
type: SequenceAnnotation
persistent identity: annotation2432567
display id: annotation2432567
version: 1
top level: BBa_K1763003
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1763003/annotation2432567/range2432567/1

https://synbiohub.org/public/igem/BBa_K1763003/annotation2432567/range2432567/1
type: Range
persistent identity: range2432567
display id: range2432567
version: 1
direction: direction/forward
top level: BBa_K1763003
start: 1
end: 6
orientation: inline

MaSp2 Core Sequence
type: SequenceAnnotation
persistent identity: annotation2472921
display id: annotation2472921
version: 1
top level: BBa_K1763003
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1763003/annotation2472921/range2472921/1

https://synbiohub.org/public/igem/BBa_K1763003/annotation2472921/range2472921/1
type: Range
persistent identity: range2472921
display id: range2472921
version: 1
direction: direction/forward
top level: BBa_K1763003
start: 10
end: 108
orientation: inline

BsaI reconition site (reverse)
type: SequenceAnnotation
persistent identity: annotation2432568
display id: annotation2432568
version: 1
top level: BBa_K1763003
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1763003/annotation2432568/range2432568/1

https://synbiohub.org/public/igem/BBa_K1763003/annotation2432568/range2432568/1
type: Range
persistent identity: range2432568
display id: range2432568
version: 1
direction: direction/reverse
top level: BBa_K1763003
start: 114
end: 119
orientation: inline

BBa_K1085024
type: ComponentDefinition
persistent identity: BBa K1085024
display id: BBa_K1085024
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS Start-codon EstA Strep-tag SpiderSilkSubunitN1 SpiderSilkSubunitN2
creator: Claudio Tiecher
created: 2013-09-15T11:00:00Z
modified: 2016-02-10T11:31:03Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _1395_
m user id: 4206
owner id: 16111
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: blabla
mutable notes: blabla
mutable provenance: blabla
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1085012
component: BBa_K1085006
sequence annotation: BBa_K1085006
sequence annotation: BBa_K1085012
sequence: https://synbiohub.org/public/igem/BBa_K1085024_sequence/1

BBa_K1085012
type: Component
persistent identity: component2347024
display id: component2347024
version: 1
top level: BBa_K1085024
access: public
definition: BBa_K1085012

BBa_K1085006
type: Component
persistent identity: component2347026
display id: component2347026
version: 1
top level: BBa_K1085024
access: public
definition: BBa_K1085006

BBa_K1085006
type: SequenceAnnotation
persistent identity: annotation2347026
display id: annotation2347026
version: 1
top level: BBa_K1085024
location: https://synbiohub.org/public/igem/BBa_K1085024/annotation2347026/range2347026/1
component: BBa_K1085006

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type: Range
persistent identity: range2347026
display id: range2347026
version: 1
direction: direction/reverse
top level: BBa_K1085024
start: 847
end: 975
orientation: inline

BBa_K1085012
type: SequenceAnnotation
persistent identity: annotation2347024
display id: annotation2347024
version: 1
top level: BBa_K1085024
location: https://synbiohub.org/public/igem/BBa_K1085024/annotation2347024/range2347024/1
component: BBa_K1085012

https://synbiohub.org/public/igem/BBa_K1085024/annotation2347024/range2347024/1
type: Range
persistent identity: range2347024
display id: range2347024
version: 1
direction: direction/reverse
top level: BBa_K1085024
start: 1
end: 838
orientation: inline

BBa_K1085040
type: ComponentDefinition
persistent identity: BBa K1085040
display id: BBa_K1085040
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: MotB Strep-tag SilkSubunitN1 SilkSubunitN2
creator: Mirjam Groenewold
created: 2013-09-16T11:00:00Z
modified: 2016-02-10T11:30:48Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _1395_
m user id: 4206
owner id: 16089
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: bla
mutable notes: bla
mutable provenance: bla
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1085006
component: BBa_K1085034
sequence annotation: BBa_K1085034
sequence annotation: BBa_K1085006
sequence: https://synbiohub.org/public/igem/BBa_K1085040_sequence/1

BBa_K1085006
type: Component
persistent identity: component2349208
display id: component2349208
version: 1
top level: BBa_K1085040
access: public
definition: BBa_K1085006

BBa_K1085034
type: Component
persistent identity: component2349206
display id: component2349206
version: 1
top level: BBa_K1085040
access: public
definition: https://synbiohub.org/public/igem/BBa_K1085034/1

BBa_K1085034
type: SequenceAnnotation
persistent identity: annotation2349206
display id: annotation2349206
version: 1
top level: BBa_K1085040
location: https://synbiohub.org/public/igem/BBa_K1085040/annotation2349206/range2349206/1
component: BBa_K1085034

https://synbiohub.org/public/igem/BBa_K1085040/annotation2349206/range2349206/1
type: Range
persistent identity: range2349206
display id: range2349206
version: 1
direction: direction/reverse
top level: BBa_K1085040
start: 1
end: 309
orientation: inline

BBa_K1085006
type: SequenceAnnotation
persistent identity: annotation2349208
display id: annotation2349208
version: 1
top level: BBa_K1085040
location: https://synbiohub.org/public/igem/BBa_K1085040/annotation2349208/range2349208/1
component: BBa_K1085006

https://synbiohub.org/public/igem/BBa_K1085040/annotation2349208/range2349208/1
type: Range
persistent identity: range2349208
display id: range2349208
version: 1
direction: direction/reverse
top level: BBa_K1085040
start: 318
end: 446
orientation: inline

BBa_K1085012
type: ComponentDefinition
persistent identity: BBa K1085012
display id: BBa_K1085012
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS Start-codon EstA Strep-tag SpiderSilkSubunitN1
creator: Claudio Tiecher
created: 2013-09-09T11:00:00Z
modified: 2016-02-10T01:09:46Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _1395_
m user id: 4206
owner id: 16089
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This BioBrick contains the coding sequence for part of the spider silk protein (SubunitN1) with a Bacillus Subtilis ribosome binding site (RBS), a coding sequence for the signal protein EstA and a Strep-tag infront of it. The RBS is there to allow the ribosomes to bind and start to translate the DNA. The EstA codes for a signal protein that that is there to fasilitate secretion of the protein. The Strep-tag, fused at the N terminal, is used both as a binding tag to use to inidicate its production and as a binding component to biotin. SubunitN1 codes for a spider silk protein that is optimized for maximal expression in Bacillus subtillus 168. The primary goal of the algorithm was to optimize the codon selection based on their availbility scores. The secondary goal was to prevent the formation of secondary RNA structures in close proximity to the RBS, and the tetrary goal was to minimize the number of restriction sites.
mutable notes: The DNA sequence was codon-optimized for Bacillus Subtilis. Moreover the nucleotide sequence was optimized in order to accomplish the production standard of IDT. A couple of optimized codons were affected by this.
mutable provenance: The amino acids sequence of the silk protein was obtained from MaSp2. The DNA sequence was synthesized by Integrated DNA Technologies (IDT).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: starting codon (ATG)
sequence annotation: RBS
sequence annotation: estA (Bac. sub.)
sequence annotation: strep
sequence annotation: spidersilk subunit N1
sequence: https://synbiohub.org/public/igem/BBa_K1085012_sequence/1

starting codon (ATG)
type: SequenceAnnotation
persistent identity: annotation2362250
display id: annotation2362250
version: 1
top level: BBa_K1085012
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1085012/annotation2362250/range2362250/1

https://synbiohub.org/public/igem/BBa_K1085012/annotation2362250/range2362250/1
type: Range
persistent identity: range2362250
display id: range2362250
version: 1
direction: direction/forward
top level: BBa_K1085012
start: 14
end: 16
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2362249
display id: annotation2362249
version: 1
top level: BBa_K1085012
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1085012/annotation2362249/range2362249/1

https://synbiohub.org/public/igem/BBa_K1085012/annotation2362249/range2362249/1
type: Range
persistent identity: range2362249
display id: range2362249
version: 1
direction: direction/forward
top level: BBa_K1085012
start: 1
end: 6
orientation: inline

estA (Bac. sub.)
type: SequenceAnnotation
persistent identity: annotation2362251
display id: annotation2362251
version: 1
top level: BBa_K1085012
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1085012/annotation2362251/range2362251/1

https://synbiohub.org/public/igem/BBa_K1085012/annotation2362251/range2362251/1
type: Range
persistent identity: range2362251
display id: range2362251
version: 1
direction: direction/forward
top level: BBa_K1085012
start: 17
end: 649
orientation: inline

strep
type: SequenceAnnotation
persistent identity: annotation2362252
display id: annotation2362252
version: 1
top level: BBa_K1085012
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1085012/annotation2362252/range2362252/1

https://synbiohub.org/public/igem/BBa_K1085012/annotation2362252/range2362252/1
type: Range
persistent identity: range2362252
display id: range2362252
version: 1
direction: direction/forward
top level: BBa_K1085012
start: 656
end: 679
orientation: inline

spidersilk subunit N1
type: SequenceAnnotation
persistent identity: annotation2362253
display id: annotation2362253
version: 1
top level: BBa_K1085012
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1085012/annotation2362253/range2362253/1

https://synbiohub.org/public/igem/BBa_K1085012/annotation2362253/range2362253/1
type: Range
persistent identity: range2362253
display id: range2362253
version: 1
direction: direction/forward
top level: BBa_K1085012
start: 698
end: 838
orientation: inline

BBa_K1085029
type: ComponentDefinition
persistent identity: BBa K1085029
display id: BBa_K1085029
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: FliZ Strep-tag SpiderSilkSubunitE1
creator: Mirjam Groenewold
created: 2013-09-16T11:00:00Z
modified: 2016-02-10T01:10:14Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _1395_
m user id: 4206
owner id: 16089
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: FliZ Strep-tag SpiderSilkSubunitE1
mutable notes: bla
mutable provenance: bla
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: SS-FlitZ
sequence annotation: Strep-Tag
sequence annotation: E1
sequence: https://synbiohub.org/public/igem/BBa_K1085029_sequence/1

SS-FlitZ
type: SequenceAnnotation
persistent identity: annotation2367937
display id: annotation2367937
version: 1
top level: BBa_K1085029
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1085029/annotation2367937/range2367937/1

https://synbiohub.org/public/igem/BBa_K1085029/annotation2367937/range2367937/1
type: Range
persistent identity: range2367937
display id: range2367937
version: 1
direction: direction/forward
top level: BBa_K1085029
start: 1
end: 81
orientation: inline

Strep-Tag
type: SequenceAnnotation
persistent identity: annotation2367938
display id: annotation2367938
version: 1
top level: BBa_K1085029
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1085029/annotation2367938/range2367938/1

https://synbiohub.org/public/igem/BBa_K1085029/annotation2367938/range2367938/1
type: Range
persistent identity: range2367938
display id: range2367938
version: 1
direction: direction/forward
top level: BBa_K1085029
start: 88
end: 111
orientation: inline

E1
type: SequenceAnnotation
persistent identity: annotation2367939
display id: annotation2367939
version: 1
top level: BBa_K1085029
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1085029/annotation2367939/range2367939/1

https://synbiohub.org/public/igem/BBa_K1085029/annotation2367939/range2367939/1
type: Range
persistent identity: range2367939
display id: range2367939
version: 1
direction: direction/forward
top level: BBa_K1085029
start: 130
end: 339
orientation: inline

BBa_K1085018
type: ComponentDefinition
persistent identity: BBa K1085018
display id: BBa_K1085018
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS Start-codon SpiderSilkSubunitE1 SpiderSilkSubunitE2 SilkTail Strep-tag stop-codon
creator: Claudio Tiecher
created: 2013-09-15T11:00:00Z
modified: 2016-02-10T11:28:19Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1395_
m user id: 4206
owner id: 16111
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: blablabla
mutable notes: blablabla
mutable provenance: blabla
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1085009
component: BBa_K1085001
component: BBa_K1085002
sequence annotation: BBa_K1085002
sequence annotation: BBa_K1085009
sequence annotation: BBa_K1085001
sequence: https://synbiohub.org/public/igem/BBa_K1085018_sequence/1

BBa_K1085009
type: Component
persistent identity: component2349178
display id: component2349178
version: 1
top level: BBa_K1085018
access: public
definition: https://synbiohub.org/public/igem/BBa_K1085009/1

BBa_K1085001
type: Component
persistent identity: component2349173
display id: component2349173
version: 1
top level: BBa_K1085018
access: public
definition: BBa_K1085001

BBa_K1085002
type: Component
persistent identity: component2349175
display id: component2349175
version: 1
top level: BBa_K1085018
access: public
definition: BBa_K1085002

BBa_K1085002
type: SequenceAnnotation
persistent identity: annotation2349175
display id: annotation2349175
version: 1
top level: BBa_K1085018
location: https://synbiohub.org/public/igem/BBa_K1085018/annotation2349175/range2349175/1
component: BBa_K1085002

https://synbiohub.org/public/igem/BBa_K1085018/annotation2349175/range2349175/1
type: Range
persistent identity: range2349175
display id: range2349175
version: 1
direction: direction/reverse
top level: BBa_K1085018
start: 235
end: 444
orientation: inline

BBa_K1085009
type: SequenceAnnotation
persistent identity: annotation2349178
display id: annotation2349178
version: 1
top level: BBa_K1085018
location: https://synbiohub.org/public/igem/BBa_K1085018/annotation2349178/range2349178/1
component: BBa_K1085009

https://synbiohub.org/public/igem/BBa_K1085018/annotation2349178/range2349178/1
type: Range
persistent identity: range2349178
display id: range2349178
version: 1
direction: direction/reverse
top level: BBa_K1085018
start: 453
end: 767
orientation: inline

BBa_K1085001
type: SequenceAnnotation
persistent identity: annotation2349173
display id: annotation2349173
version: 1
top level: BBa_K1085018
location: https://synbiohub.org/public/igem/BBa_K1085018/annotation2349173/range2349173/1
component: BBa_K1085001

https://synbiohub.org/public/igem/BBa_K1085018/annotation2349173/range2349173/1
type: Range
persistent identity: range2349173
display id: range2349173
version: 1
direction: direction/reverse
top level: BBa_K1085018
start: 1
end: 226
orientation: inline

BBa_K1085000
type: ComponentDefinition
persistent identity: BBa K1085000
display id: BBa_K1085000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS Start-codon Strep-tag SpiderSilkSubunitE1
creator: Claudio Tiecher
created: 2013-08-29T11:00:00Z
modified: 2016-02-10T01:07:41Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1395_
m user id: 4206
owner id: 16111
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: The part contains the coding sequence for part of the spider silk protein together with Bacillus Subtilis ribosome binding site (RBS) and the Strep-tag.
mutable notes: The DNA sequence was codon-optimized for Bacillus Subtilis. Moreover the nucleotide sequence was optimized in order to accomplish the production standard of IDT. A couple of optimized codons were affected by this.
mutable provenance: The amino acids sequence of the silk protein was obtained from the paper by Brooks et al. (2008). The DNA sequence was synthesized by Integrated DNA Technologies (IDT).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: RBS
sequence annotation: starting codon (ATG)
sequence annotation: Strep
sequence annotation: spidersilk subunit E1
sequence: https://synbiohub.org/public/igem/BBa_K1085000_sequence/1

RBS
type: SequenceAnnotation
persistent identity: annotation2333669
display id: annotation2333669
version: 1
top level: BBa_K1085000
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1085000/annotation2333669/range2333669/1

https://synbiohub.org/public/igem/BBa_K1085000/annotation2333669/range2333669/1
type: Range
persistent identity: range2333669
display id: range2333669
version: 1
direction: direction/forward
top level: BBa_K1085000
start: 1
end: 6
orientation: inline

starting codon (ATG)
type: SequenceAnnotation
persistent identity: annotation2338313
display id: annotation2338313
version: 1
top level: BBa_K1085000
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1085000/annotation2338313/range2338313/1

https://synbiohub.org/public/igem/BBa_K1085000/annotation2338313/range2338313/1
type: Range
persistent identity: range2338313
display id: range2338313
version: 1
direction: direction/forward
top level: BBa_K1085000
start: 14
end: 16
orientation: inline

Strep
type: SequenceAnnotation
persistent identity: annotation2333670
display id: annotation2333670
version: 1
top level: BBa_K1085000
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1085000/annotation2333670/range2333670/1

https://synbiohub.org/public/igem/BBa_K1085000/annotation2333670/range2333670/1
type: Range
persistent identity: range2333670
display id: range2333670
version: 1
direction: direction/forward
top level: BBa_K1085000
start: 17
end: 40
orientation: inline

spidersilk subunit E1
type: SequenceAnnotation
persistent identity: annotation2333671
display id: annotation2333671
version: 1
top level: BBa_K1085000
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1085000/annotation2333671/range2333671/1

https://synbiohub.org/public/igem/BBa_K1085000/annotation2333671/range2333671/1
type: Range
persistent identity: range2333671
display id: range2333671
version: 1
direction: direction/forward
top level: BBa_K1085000
start: 59
end: 268
orientation: inline

BBa_K1763015
type: ComponentDefinition
persistent identity: BBa K1763015
display id: BBa_K1763015
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: T7 Promoter+Silk+sfGFP
creator: Megan Satyadi
created: 2015-08-21T11:00:00Z
modified: 2016-02-10T11:25:59Z
discontinued: false
dominant: false
group u list: _2185_
m user id: 4206
owner id: 27400
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This part is a fusion of the Apis mellifera (Western Honey Bee) silk protein coding region and the superfolder GFP coding region. A T7 promoter regulatory element has been added upstream, along with a ribosomal binding site. (in progress)
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence: https://synbiohub.org/public/igem/BBa_K1763015_sequence/1

BBa_K1085005
type: ComponentDefinition
persistent identity: BBa K1085005
display id: BBa_K1085005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS Start-codon SpiderSilkSubunitN1
creator: Claudio Tiecher
created: 2013-09-09T11:00:00Z
modified: 2016-02-10T01:11:44Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1395_
m user id: 0
m user id: 4206
owner id: 16089
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This BioBrick contains the coding sequence for part of the spider silk protein (SubunitN1) with Bacillus Subtilis ribosome binding site (RBS) infront of it. The RBS is there to allow the ribosomes to bind and start to translate the DNA. SubunitN1 codes for a spider silk protein that is optimized for maximal expression in Bacillus subtillus 168. The primary goal of the algorithm was to optimize the codon selection based on their availbility scores. The secondary goal was to prevent the formation of secondary RNA structures in close proximity to the RBS, and the tetrary goal was to minimize the number of restriction sites.
mutable notes: The DNA sequence was codon-optimized for Bacillus Subtilis. Moreover the nucleotide sequence was optimized in order to accomplish the production standard of IDT. A couple of optimized codons were affected by this.
mutable provenance: The amino acids sequence of the silk protein was obtained from MaSp2. The DNA sequence was synthesized by Integrated DNA Technologies (IDT).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: spidersilk subunit N1
sequence annotation: starting codon (ATG)
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_K1085005_sequence/1

spidersilk subunit N1
type: SequenceAnnotation
persistent identity: annotation2339054
display id: annotation2339054
version: 1
top level: BBa_K1085005
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1085005/annotation2339054/range2339054/1

https://synbiohub.org/public/igem/BBa_K1085005/annotation2339054/range2339054/1
type: Range
persistent identity: range2339054
display id: range2339054
version: 1
direction: direction/forward
top level: BBa_K1085005
start: 17
end: 157
orientation: inline

starting codon (ATG)
type: SequenceAnnotation
persistent identity: annotation2339053
display id: annotation2339053
version: 1
top level: BBa_K1085005
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1085005/annotation2339053/range2339053/1

https://synbiohub.org/public/igem/BBa_K1085005/annotation2339053/range2339053/1
type: Range
persistent identity: range2339053
display id: range2339053
version: 1
direction: direction/forward
top level: BBa_K1085005
start: 14
end: 16
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2339052
display id: annotation2339052
version: 1
top level: BBa_K1085005
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1085005/annotation2339052/range2339052/1

https://synbiohub.org/public/igem/BBa_K1085005/annotation2339052/range2339052/1
type: Range
persistent identity: range2339052
display id: range2339052
version: 1
direction: direction/forward
top level: BBa_K1085005
start: 1
end: 6
orientation: inline

BBa_K1085010
type: ComponentDefinition
persistent identity: BBa K1085010
display id: BBa_K1085010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS Start-codon EstA Strep-tag SpiderSilkSubunitE1
creator: Claudio Tiecher
created: 2013-09-09T11:00:00Z
modified: 2016-02-10T01:09:30Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _1395_
m user id: 4206
owner id: 16089
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This BioBrick contains the coding sequence for part of the spider silk protein (SubunitE1) with a Bacillus Subtilis ribosome binding site (RBS), a coding sequence for the signal protein EstA and a Strep-tag infront of it. The RBS is there to allow the ribosomes to bind and start to translate the DNA. The EstA codes for a signal protein that that is there to fasilitate secretion of the protein. The Strep-tag, fused at the N terminal, is used both as a binding tag to use to inidicate its production and as a binding component to biotin. SubunitE1 codes for a spider silk protein that is optimized for maximal expression in Bacillus subtillus 168. The primary goal of the algorithm was to optimize the codon selection based on their availbility scores. The secondary goal was to prevent the formation of secondary RNA structures in close proximity to the RBS, and the tetrary goal was to minimize the number of restriction sites. The amino acid sequence is based on the adapted MaSp2 sequences discribed in the paper of Brooks et. al. 2008.
mutable notes: The DNA sequence was codon-optimized for Bacillus Subtilis. Moreover the nucleotide sequence was optimized in order to accomplish the production standard of IDT. A couple of optimized codons were affected by this.
mutable provenance: The amino acid sequence of the silk protein was obtained from the paper by Brooks et al. (2008). The DNA sequence was synthesized by Integrated DNA Technologies (IDT).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: RBS
sequence annotation: starting codon (ATG)
sequence annotation: strep
sequence annotation: spidersilk subunit E1
sequence annotation: estA (Bac. sub.)
sequence: https://synbiohub.org/public/igem/BBa_K1085010_sequence/1

RBS
type: SequenceAnnotation
persistent identity: annotation2339063
display id: annotation2339063
version: 1
top level: BBa_K1085010
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1085010/annotation2339063/range2339063/1

https://synbiohub.org/public/igem/BBa_K1085010/annotation2339063/range2339063/1
type: Range
persistent identity: range2339063
display id: range2339063
version: 1
direction: direction/forward
top level: BBa_K1085010
start: 1
end: 6
orientation: inline

starting codon (ATG)
type: SequenceAnnotation
persistent identity: annotation2362247
display id: annotation2362247
version: 1
top level: BBa_K1085010
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1085010/annotation2362247/range2362247/1

https://synbiohub.org/public/igem/BBa_K1085010/annotation2362247/range2362247/1
type: Range
persistent identity: range2362247
display id: range2362247
version: 1
direction: direction/forward
top level: BBa_K1085010
start: 14
end: 16
orientation: inline

strep
type: SequenceAnnotation
persistent identity: annotation2339066
display id: annotation2339066
version: 1
top level: BBa_K1085010
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1085010/annotation2339066/range2339066/1

https://synbiohub.org/public/igem/BBa_K1085010/annotation2339066/range2339066/1
type: Range
persistent identity: range2339066
display id: range2339066
version: 1
direction: direction/forward
top level: BBa_K1085010
start: 656
end: 679
orientation: inline

spidersilk subunit E1
type: SequenceAnnotation
persistent identity: annotation2339067
display id: annotation2339067
version: 1
top level: BBa_K1085010
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1085010/annotation2339067/range2339067/1

https://synbiohub.org/public/igem/BBa_K1085010/annotation2339067/range2339067/1
type: Range
persistent identity: range2339067
display id: range2339067
version: 1
direction: direction/forward
top level: BBa_K1085010
start: 698
end: 907
orientation: inline

estA (Bac. sub.)
type: SequenceAnnotation
persistent identity: annotation2339064
display id: annotation2339064
version: 1
top level: BBa_K1085010
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1085010/annotation2339064/range2339064/1

https://synbiohub.org/public/igem/BBa_K1085010/annotation2339064/range2339064/1
type: Range
persistent identity: range2339064
display id: range2339064
version: 1
direction: direction/forward
top level: BBa_K1085010
start: 17
end: 649
orientation: inline

BBa_K1085001
type: ComponentDefinition
persistent identity: BBa K1085001
display id: BBa_K1085001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS Start-codon SpiderSilkSubunitE1
creator: Claudio Tiecher
created: 2013-09-09T11:00:00Z
modified: 2016-02-10T01:07:59Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1395_
m user id: 4206
owner id: 16089
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This BioBrick contains the coding sequence for part of the spider silk protein (SubunitE1) with Bacillus Subtilis ribosome binding site (RBS) infront of it. The RBS is there to allow the ribosomes to bind and start to translate the DNA. SubunitE1 codes for a spider silk protein that is optimized to prevent the forming of secundary RNA structures in close proximity to the RBS. This is given priority over reducing the number of restriction sites. However, the primary goal of the algorithm was still to optimize the codon selection based on their availbility scores.
mutable notes: The DNA sequence was codon-optimized for Bacillus Subtilis. Moreover the nucleotide sequence was optimized in order to accomplish the production standard of IDT. A couple of optimized codons were affected by this.
mutable provenance: The amino acids sequence of the silk protein was obtained from the paper by Brooks et al. (2008). The DNA sequence was synthesized by Integrated DNA Technologies (IDT).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: spidersilk subunit E1
sequence annotation: starting codon (ATG)
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_K1085001_sequence/1

spidersilk subunit E1
type: SequenceAnnotation
persistent identity: annotation2338312
display id: annotation2338312
version: 1
top level: BBa_K1085001
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1085001/annotation2338312/range2338312/1

https://synbiohub.org/public/igem/BBa_K1085001/annotation2338312/range2338312/1
type: Range
persistent identity: range2338312
display id: range2338312
version: 1
direction: direction/forward
top level: BBa_K1085001
start: 17
end: 226
orientation: inline

starting codon (ATG)
type: SequenceAnnotation
persistent identity: annotation2338314
display id: annotation2338314
version: 1
top level: BBa_K1085001
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1085001/annotation2338314/range2338314/1

https://synbiohub.org/public/igem/BBa_K1085001/annotation2338314/range2338314/1
type: Range
persistent identity: range2338314
display id: range2338314
version: 1
direction: direction/forward
top level: BBa_K1085001
start: 14
end: 16
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2338309
display id: annotation2338309
version: 1
top level: BBa_K1085001
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1085001/annotation2338309/range2338309/1

https://synbiohub.org/public/igem/BBa_K1085001/annotation2338309/range2338309/1
type: Range
persistent identity: range2338309
display id: range2338309
version: 1
direction: direction/forward
top level: BBa_K1085001
start: 1
end: 6
orientation: inline

PatgB4HT
type: ComponentDefinition
persistent identity: BBa K844016
display id: BBa_K844016
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Spider Silk Generator - 4x "B" Silk Construct with His Tag
creator: Brian Smith
created: 2012-10-01T11:00:00Z
modified: 2016-02-10T11:27:13Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1104_
m user id: 4206
owner id: 14072
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: Protein generator part for Spider Silk. Protein is a 4x fusion of spider silk "B" subunits, which were codon optimized to use a reduced set of tRNA codons (1 to 2 codons per amino acid). Contains a lactose/IPTG inducible , RBS, and a 10x-His tag for purification of protein. IMPORTANT NOTE: This part uses Assembly Standard #23 (Silver Fusion) scar sites, which the sequence data below will not reflect (it shows Assembly Standard #10 scars).
mutable notes: none.
mutable provenance: Silver Fusion assembly of basic parts
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K844004
component: BBa_K844008
component: BBa_K844000
component: BBa_K208010
sequence annotation: BBa_K208010
sequence annotation: BBa_K844004
sequence annotation: BBa_K844008
sequence annotation: BBa_K844000
sequence: https://synbiohub.org/public/igem/BBa_K844016_sequence/1

BBa_K844004
type: Component
persistent identity: component2211639
display id: component2211639
version: 1
top level: PatgB4HT
access: public
definition: https://synbiohub.org/public/igem/BBa_K844004/1

BBa_K844008
type: Component
persistent identity: component2211582
display id: component2211582
version: 1
top level: PatgB4HT
access: public
definition: BBa_K844008

BBa_K844000
type: Component
persistent identity: component2211643
display id: component2211643
version: 1
top level: PatgB4HT
access: public
definition: https://synbiohub.org/public/igem/BBa_K844000/1

BBa_K208010
type: Component
persistent identity: component2211562
display id: component2211562
version: 1
top level: PatgB4HT
access: public
definition: https://synbiohub.org/public/igem/BBa_K208010/1

BBa_K844004
type: Component
persistent identity: component2211601
display id: component2211601
version: 1
top level: PatgB4HT
access: public
definition: https://synbiohub.org/public/igem/BBa_K844004/1

BBa_K844004
type: Component
persistent identity: component2211620
display id: component2211620
version: 1
top level: PatgB4HT
access: public
definition: https://synbiohub.org/public/igem/BBa_K844004/1

BBa_K208010
type: SequenceAnnotation
persistent identity: annotation2211562
display id: annotation2211562
version: 1
top level: PatgB4HT
location: https://synbiohub.org/public/igem/BBa_K844016/annotation2211562/range2211562/1
component: BBa_K208010

https://synbiohub.org/public/igem/BBa_K844016/annotation2211562/range2211562/1
type: Range
persistent identity: range2211562
display id: range2211562
version: 1
direction: direction/reverse
top level: PatgB4HT
start: 1
end: 220
orientation: inline

BBa_K844004
type: SequenceAnnotation
persistent identity: annotation2211639
display id: annotation2211639
version: 1
top level: PatgB4HT
location: https://synbiohub.org/public/igem/BBa_K844016/annotation2211639/range2211639/1
component: BBa_K844004

https://synbiohub.org/public/igem/BBa_K844016/annotation2211639/range2211639/1
type: Range
persistent identity: range2211639
display id: range2211639
version: 1
direction: direction/reverse
top level: PatgB4HT
start: 866
end: 1069
orientation: inline

BBa_K844004
type: SequenceAnnotation
persistent identity: annotation2211601
display id: annotation2211601
version: 1
top level: PatgB4HT
location: https://synbiohub.org/public/igem/BBa_K844016/annotation2211601/range2211601/1
component: BBa_K844004

https://synbiohub.org/public/igem/BBa_K844016/annotation2211601/range2211601/1
type: Range
persistent identity: range2211601
display id: range2211601
version: 1
direction: direction/reverse
top level: PatgB4HT
start: 442
end: 645
orientation: inline

BBa_K844008
type: SequenceAnnotation
persistent identity: annotation2211582
display id: annotation2211582
version: 1
top level: PatgB4HT
location: https://synbiohub.org/public/igem/BBa_K844016/annotation2211582/range2211582/1
component: BBa_K844008

https://synbiohub.org/public/igem/BBa_K844016/annotation2211582/range2211582/1
type: Range
persistent identity: range2211582
display id: range2211582
version: 1
direction: direction/reverse
top level: PatgB4HT
start: 227
end: 433
orientation: inline

BBa_K844000
type: SequenceAnnotation
persistent identity: annotation2211643
display id: annotation2211643
version: 1
top level: PatgB4HT
location: https://synbiohub.org/public/igem/BBa_K844016/annotation2211643/range2211643/1
component: BBa_K844000

https://synbiohub.org/public/igem/BBa_K844016/annotation2211643/range2211643/1
type: Range
persistent identity: range2211643
display id: range2211643
version: 1
direction: direction/reverse
top level: PatgB4HT
start: 1078
end: 1113
orientation: inline

BBa_K844004
type: SequenceAnnotation
persistent identity: annotation2211620
display id: annotation2211620
version: 1
top level: PatgB4HT
location: https://synbiohub.org/public/igem/BBa_K844016/annotation2211620/range2211620/1
component: BBa_K844004

https://synbiohub.org/public/igem/BBa_K844016/annotation2211620/range2211620/1
type: Range
persistent identity: range2211620
display id: range2211620
version: 1
direction: direction/reverse
top level: PatgB4HT
start: 654
end: 857
orientation: inline

BBa_K1085006
type: ComponentDefinition
persistent identity: BBa K1085006
display id: BBa_K1085006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: SpiderSilkSubunitN2
creator: Claudio Tiecher
created: 2013-09-09T11:00:00Z
modified: 2016-02-10T01:07:20Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1395_
m user id: 0
m user id: 4206
owner id: 16089
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This BioBrick contains the coding sequence for part of the spider silk protein (SubunitN2). SubunitN2 codes for a spider silk protein that is optimized for maximal expression in Bacillus subtillus 168. The primary goal of the algorithm was to optimize the codon selection based on their availbility scores. The secondary goal was to minimize the number of restriction sites, and the tetrary goal was to prevent the formation of secondary RNA structures in close proximity to the RBS.
mutable notes: The DNA sequence was codon-optimized for Bacillus Subtilis. Moreover the nucleotide sequence was optimized in order to accomplish the production standard of IDT. A couple of optimized codons were affected by this.
mutable provenance: The amino acids sequence of the silk protein was obtained from MaSp2. The DNA sequence was synthesized by Integrated DNA Technologies (IDT).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: spidersilk subunit N2
sequence: https://synbiohub.org/public/igem/BBa_K1085006_sequence/1

spidersilk subunit N2
type: SequenceAnnotation
persistent identity: annotation2339055
display id: annotation2339055
version: 1
top level: BBa_K1085006
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1085006/annotation2339055/range2339055/1

https://synbiohub.org/public/igem/BBa_K1085006/annotation2339055/range2339055/1
type: Range
persistent identity: range2339055
display id: range2339055
version: 1
direction: direction/forward
top level: BBa_K1085006
start: 1
end: 129
orientation: inline

BBa_K1085015
type: ComponentDefinition
persistent identity: BBa K1085015
display id: BBa_K1085015
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS Start-codon Strep-tag SpiderSilkSubunitE1 SpiderSilkSubunitE2
creator: Claudio Tiecher
created: 2013-09-09T11:00:00Z
modified: 2016-02-10T11:31:42Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _1395_
m user id: 4206
owner id: 16089
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This BioBrick contains the coding sequence for two parts of the spider silk proteins (SubunitE1 and SubunitE2) with a Bacillus Subtilis ribosome binding site (RBS) and a Strep-tag infront of it. The RBS is there to allow the ribosomes to bind and start to translate the DNA. The Strep-tag, fused at the N terminal, is used both as a binding tag to use to inidicate its production and as a binding component to biotin. SubunitE1 codes for a spider silk protein that is optimized for maximal expression in Bacillus subtillus 168. The primary goal of the algorithm was to optimize the codon selection based on their availbility scores. The secondary goal was to prevent the formation of secondary RNA structures in close proximity to the RBS, and the tetrary goal was to minimize the number of restriction sites. SubunitE2 codes for a spider silk protein that is optimized for maximal expression in Bacillus subtillus 168. The primary goal of the algorithm was to optimize the codon selection based on their availbility scores. The secondary goal was to minimize the number of restriction sites, and the tetrary goal was to prevent the formation of secondary RNA structures in close proximity to the RBS.
mutable notes: The DNA sequence was codon-optimized for Bacillus Subtilis. Moreover the nucleotide sequence was optimized in order to accomplish the production standard of IDT. A couple of optimized codons were affected by this.
mutable provenance: The amino acids sequence of the silk protein was obtained from the paper by Brooks et al. (2008). The DNA sequence was synthesized by Integrated DNA Technologies (IDT).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1085000
component: BBa_K1085002
sequence annotation: BBa_K1085000
sequence annotation: BBa_K1085002
sequence: https://synbiohub.org/public/igem/BBa_K1085015_sequence/1

BBa_K1085000
type: Component
persistent identity: component2361170
display id: component2361170
version: 1
top level: BBa_K1085015
access: public
definition: BBa_K1085000

BBa_K1085002
type: Component
persistent identity: component2361172
display id: component2361172
version: 1
top level: BBa_K1085015
access: public
definition: BBa_K1085002

BBa_K1085000
type: SequenceAnnotation
persistent identity: annotation2361170
display id: annotation2361170
version: 1
top level: BBa_K1085015
location: https://synbiohub.org/public/igem/BBa_K1085015/annotation2361170/range2361170/1
component: BBa_K1085000

https://synbiohub.org/public/igem/BBa_K1085015/annotation2361170/range2361170/1
type: Range
persistent identity: range2361170
display id: range2361170
version: 1
direction: direction/reverse
top level: BBa_K1085015
start: 1
end: 268
orientation: inline

BBa_K1085002
type: SequenceAnnotation
persistent identity: annotation2361172
display id: annotation2361172
version: 1
top level: BBa_K1085015
location: https://synbiohub.org/public/igem/BBa_K1085015/annotation2361172/range2361172/1
component: BBa_K1085002

https://synbiohub.org/public/igem/BBa_K1085015/annotation2361172/range2361172/1
type: Range
persistent identity: range2361172
display id: range2361172
version: 1
direction: direction/reverse
top level: BBa_K1085015
start: 277
end: 486
orientation: inline

BBa_K1763422
type: ComponentDefinition
persistent identity: BBa K1763422
display id: BBa_K1763422
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Apis Mellifera silk fused to SpyCatcher
creator: Daniel Cancilla
created: 2015-09-13T11:00:00Z
modified: 2016-02-10T11:32:53Z
discontinued: false
dominant: false
group u list: _2185_
m user id: 4206
owner id: 18035
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This part is a fusion between the coding region of the honeybee silk protein and SpyCatcher protein which has a high affinity for SpyTag
mutable notes: NA
mutable provenance: Synthesized via IDT
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence: https://synbiohub.org/public/igem/BBa_K1763422_sequence/1

BBa_K1085008
type: ComponentDefinition
persistent identity: BBa K1085008
display id: BBa_K1085008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: SilkTail Stop-codon
creator: Claudio Tiecher
created: 2013-09-09T11:00:00Z
modified: 2016-02-10T01:11:21Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1395_
m user id: 0
m user id: 4206
owner id: 16089
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This BioBrick contains the coding sequence for silk tail and ends with a stop codon. The Tail ....
mutable notes: The DNA sequence was codon-optimized for Bacillus Subtilis. Moreover the nucleotide sequence was optimized in order to accomplish the production standard of IDT. A couple of optimized codons were affected by this.
mutable provenance: The amino acid sequence of the tail was obtained from MaSp2. The DNA sequence was synthesized by Integrated DNA Technologies (IDT).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: spidersilk C-terminal domain
sequence annotation: Stop codon (TGA)
sequence: https://synbiohub.org/public/igem/BBa_K1085008_sequence/1

spidersilk C-terminal domain
type: SequenceAnnotation
persistent identity: annotation2339058
display id: annotation2339058
version: 1
top level: BBa_K1085008
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1085008/annotation2339058/range2339058/1

https://synbiohub.org/public/igem/BBa_K1085008/annotation2339058/range2339058/1
type: Range
persistent identity: range2339058
display id: range2339058
version: 1
direction: direction/forward
top level: BBa_K1085008
start: 1
end: 270
orientation: inline

Stop codon (TGA)
type: SequenceAnnotation
persistent identity: annotation2362390
display id: annotation2362390
version: 1
top level: BBa_K1085008
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1085008/annotation2362390/range2362390/1

https://synbiohub.org/public/igem/BBa_K1085008/annotation2362390/range2362390/1
type: Range
persistent identity: range2362390
display id: range2362390
version: 1
direction: direction/forward
top level: BBa_K1085008
start: 271
end: 273
orientation: inline

BBa_K1085003
type: ComponentDefinition
persistent identity: BBa K1085003
display id: BBa_K1085003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: SpiderSilkSubunitE2 Strep-tag
creator: Claudio Tiecher
created: 2013-09-09T11:00:00Z
modified: 2016-02-10T01:09:12Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1395_
m user id: 0
m user id: 4206
owner id: 16089
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This BioBrick contains the coding sequence for part of the spider silk protein (SubunitE2) and a Strep-tag behind it. SubunitE2 codes for a spider silk protein that is optimized for maximal expression in Bacillus subtillus 168. The primary goal of the algorithm was to optimize the codon selection based on their availbility scores. The secondary goal was to minimize the number of restriction sites, and the tetrary goal was to prevent the formation of secondary RNA structures in close proximity to the RBS. The amino acid sequence is based on the adapted MaSp2 sequences discribed in the paper of Brooks et. al. 2008. The Strep-tag, fused at the C terminal, is used both as a binding tag to use to inidicate its production and as a binding component to biotin.
mutable notes: The DNA sequence was codon-optimized for Bacillus Subtilis. Moreover the nucleotide sequence was optimized in order to accomplish the production standard of IDT. A couple of optimized codons were affected by this.
mutable provenance: The amino acids sequence of the silk protein was obtained from the paper by Brooks et al. (2008). The DNA sequence was synthesized by Integrated DNA Technologies (IDT).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: strep
sequence annotation: spidersilk subunit E2
sequence: https://synbiohub.org/public/igem/BBa_K1085003_sequence/1

strep
type: SequenceAnnotation
persistent identity: annotation2339045
display id: annotation2339045
version: 1
top level: BBa_K1085003
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1085003/annotation2339045/range2339045/1

https://synbiohub.org/public/igem/BBa_K1085003/annotation2339045/range2339045/1
type: Range
persistent identity: range2339045
display id: range2339045
version: 1
direction: direction/forward
top level: BBa_K1085003
start: 229
end: 252
orientation: inline

spidersilk subunit E2
type: SequenceAnnotation
persistent identity: annotation2339046
display id: annotation2339046
version: 1
top level: BBa_K1085003
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1085003/annotation2339046/range2339046/1

https://synbiohub.org/public/igem/BBa_K1085003/annotation2339046/range2339046/1
type: Range
persistent identity: range2339046
display id: range2339046
version: 1
direction: direction/forward
top level: BBa_K1085003
start: 1
end: 210
orientation: inline

BBa_K844008
type: ComponentDefinition
persistent identity: BBa K844008
display id: BBa_K844008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Spider Silk 1x Subunit "B" (Balanced tRNA codon optimized) with Met (ATG) added
creator: Andrea Halling
created: 2012-10-01T11:00:00Z
modified: 2016-02-10T11:28:32Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1104_
m user id: 4206
owner id: 13876
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Spider silk subunit optimized to use a reduced set of tRNA codons (1 or 2 codons per amino acid); contains two elasticity domains and one strength domain, also contains 5??? Met (ATG) codon.
mutable notes: none
mutable provenance: Created using mutagenesis PCR to add upstream Met (ATG) to part BBa_K844004
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Beta-spiral
sequence annotation: Linker Domain
sequence annotation: Elasticity Domain
sequence annotation: Start
sequence annotation: Beta-helix
sequence annotation: Strength Domain
sequence annotation: Beta-sprial
sequence: https://synbiohub.org/public/igem/BBa_K844008_sequence/1

Beta-spiral
type: SequenceAnnotation
persistent identity: annotation2212456
display id: annotation2212456
version: 1
top level: BBa_K844008
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K844008/annotation2212456/range2212456/1

https://synbiohub.org/public/igem/BBa_K844008/annotation2212456/range2212456/1
type: Range
persistent identity: range2212456
display id: range2212456
version: 1
direction: direction/forward
top level: BBa_K844008
start: 49
end: 63
orientation: inline

Linker Domain
type: SequenceAnnotation
persistent identity: annotation2212464
display id: annotation2212464
version: 1
top level: BBa_K844008
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K844008/annotation2212464/range2212464/1

https://synbiohub.org/public/igem/BBa_K844008/annotation2212464/range2212464/1
type: Range
persistent identity: range2212464
display id: range2212464
version: 1
direction: direction/forward
top level: BBa_K844008
start: 172
end: 188
orientation: inline

Beta-spiral
type: SequenceAnnotation
persistent identity: annotation2212457
display id: annotation2212457
version: 1
top level: BBa_K844008
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K844008/annotation2212457/range2212457/1

https://synbiohub.org/public/igem/BBa_K844008/annotation2212457/range2212457/1
type: Range
persistent identity: range2212457
display id: range2212457
version: 1
direction: direction/forward
top level: BBa_K844008
start: 72
end: 87
orientation: inline

Beta-spiral
type: SequenceAnnotation
persistent identity: annotation2212454
display id: annotation2212454
version: 1
top level: BBa_K844008
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K844008/annotation2212454/range2212454/1

https://synbiohub.org/public/igem/BBa_K844008/annotation2212454/range2212454/1
type: Range
persistent identity: range2212454
display id: range2212454
version: 1
direction: direction/forward
top level: BBa_K844008
start: 13
end: 27
orientation: inline

Beta-spiral
type: SequenceAnnotation
persistent identity: annotation2212455
display id: annotation2212455
version: 1
top level: BBa_K844008
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K844008/annotation2212455/range2212455/1

https://synbiohub.org/public/igem/BBa_K844008/annotation2212455/range2212455/1
type: Range
persistent identity: range2212455
display id: range2212455
version: 1
direction: direction/forward
top level: BBa_K844008
start: 34
end: 48
orientation: inline

Beta-spiral
type: SequenceAnnotation
persistent identity: annotation2212460
display id: annotation2212460
version: 1
top level: BBa_K844008
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K844008/annotation2212460/range2212460/1

https://synbiohub.org/public/igem/BBa_K844008/annotation2212460/range2212460/1
type: Range
persistent identity: range2212460
display id: range2212460
version: 1
direction: direction/forward
top level: BBa_K844008
start: 98
end: 113
orientation: inline

Elasticity Domain
type: SequenceAnnotation
persistent identity: annotation2212459
display id: annotation2212459
version: 1
top level: BBa_K844008
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K844008/annotation2212459/range2212459/1

https://synbiohub.org/public/igem/BBa_K844008/annotation2212459/range2212459/1
type: Range
persistent identity: range2212459
display id: range2212459
version: 1
direction: direction/forward
top level: BBa_K844008
start: 88
end: 171
orientation: inline

Start
type: SequenceAnnotation
persistent identity: annotation2212451
display id: annotation2212451
version: 1
top level: BBa_K844008
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K844008/annotation2212451/range2212451/1

https://synbiohub.org/public/igem/BBa_K844008/annotation2212451/range2212451/1
type: Range
persistent identity: range2212451
display id: range2212451
version: 1
direction: direction/forward
top level: BBa_K844008
start: 1
end: 3
orientation: inline

Beta-spiral
type: SequenceAnnotation
persistent identity: annotation2212462
display id: annotation2212462
version: 1
top level: BBa_K844008
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K844008/annotation2212462/range2212462/1

https://synbiohub.org/public/igem/BBa_K844008/annotation2212462/range2212462/1
type: Range
persistent identity: range2212462
display id: range2212462
version: 1
direction: direction/forward
top level: BBa_K844008
start: 133
end: 147
orientation: inline

Beta-spiral
type: SequenceAnnotation
persistent identity: annotation2212463
display id: annotation2212463
version: 1
top level: BBa_K844008
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K844008/annotation2212463/range2212463/1

https://synbiohub.org/public/igem/BBa_K844008/annotation2212463/range2212463/1
type: Range
persistent identity: range2212463
display id: range2212463
version: 1
direction: direction/forward
top level: BBa_K844008
start: 156
end: 171
orientation: inline

Beta-helix
type: SequenceAnnotation
persistent identity: annotation2212458
display id: annotation2212458
version: 1
top level: BBa_K844008
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K844008/annotation2212458/range2212458/1

https://synbiohub.org/public/igem/BBa_K844008/annotation2212458/range2212458/1
type: Range
persistent identity: range2212458
display id: range2212458
version: 1
direction: direction/forward
top level: BBa_K844008
start: 88
end: 97
orientation: inline

Elasticity Domain
type: SequenceAnnotation
persistent identity: annotation2212453
display id: annotation2212453
version: 1
top level: BBa_K844008
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K844008/annotation2212453/range2212453/1

https://synbiohub.org/public/igem/BBa_K844008/annotation2212453/range2212453/1
type: Range
persistent identity: range2212453
display id: range2212453
version: 1
direction: direction/forward
top level: BBa_K844008
start: 4
end: 87
orientation: inline

Strength Domain
type: SequenceAnnotation
persistent identity: annotation2212465
display id: annotation2212465
version: 1
top level: BBa_K844008
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K844008/annotation2212465/range2212465/1

https://synbiohub.org/public/igem/BBa_K844008/annotation2212465/range2212465/1
type: Range
persistent identity: range2212465
display id: range2212465
version: 1
direction: direction/forward
top level: BBa_K844008
start: 189
end: 207
orientation: inline

Beta-sprial
type: SequenceAnnotation
persistent identity: annotation2212461
display id: annotation2212461
version: 1
top level: BBa_K844008
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K844008/annotation2212461/range2212461/1

https://synbiohub.org/public/igem/BBa_K844008/annotation2212461/range2212461/1
type: Range
persistent identity: range2212461
display id: range2212461
version: 1
direction: direction/forward
top level: BBa_K844008
start: 117
end: 132
orientation: inline

Beta-helix
type: SequenceAnnotation
persistent identity: annotation2212452
display id: annotation2212452
version: 1
top level: BBa_K844008
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K844008/annotation2212452/range2212452/1

https://synbiohub.org/public/igem/BBa_K844008/annotation2212452/range2212452/1
type: Range
persistent identity: range2212452
display id: range2212452
version: 1
direction: direction/forward
top level: BBa_K844008
start: 4
end: 12
orientation: inline

Tamura
type: ComponentDefinition
persistent identity: BBa K1763444
display id: BBa_K1763444
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Bombyx mori silk co-spinning module
creator: Fasih Ahsan
created: 2015-09-14T11:00:00Z
modified: 2016-02-10T11:26:30Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2185_
m user id: 4206
owner id: 22156
owning group id: 9
status: status/Planning
bookmark: true
mutable description: Studies investigating the potential for co-spinning, a process in which the terminal domains of the functional B. mori silk protein heavy chain are fused with genes encoding functional binding domains, and subsequently are combined with native silk proteins to form crystalline fibers, have shown some success (Sponner et al., 2005). In vivo co-spinning simulations have previously been conducted using the transgene in silkworms, producing functionally fluorescent N-terminal ??? GFP- C-terminal fusions that successfully incorporate into endogenous Bombyx mori silkworm silk monomers (Kojima et. al., 2007). These N-Terminal and C-terminal regions are not only easier to clone due to their non-repetitive structures, but additionally allows for storage and assembly of endogenous ampullate spidroins (Askarieh et al., 2010). Composite silkworm-spider silk chimeras have shown improved mechanical properties, and may increase the ease in high-yield production due to the easier manipulation of silkworm silk monomers (Teule, et. al. 2012). Yet, this process still relies on the use of transgenic silkworms, which have longer developmental cycles, require larger storage and nutrient conditions, and difficulty to harvest relative to bacterial counterparts (Murphy et al., 2009). An ex situ co-spinning process has been developed for the production of composite N. clavipes ??? B. mori silk fibers with functional protein fusions in bacterial chassis (namely, E. coli). This process results in the construction of a transgene that can be modularized using restriction enzymes to incorporate a wide range of functional peptide binding sites or other proteins, including avidin-biotin binding domains, RGD-cadherin motifs, albumin binding domains, or antibody affinity domains (Jansson et al., 2014). Specifically, this composite structure focuses on the ability to express functional sfGFP in silk fibers as a marker for successful co-spinning, and verifies proper NC-sfGFP expression using SDS-PAGE a…
mutable notes: We decided to use a LacI promoter to regulate expression of the large protein construct in E. coli K12 laboratory strains, in lieu of utilizing a T7 promoter or constitutive Anderson family promoters. This is to maximize the protein yield of the co-spinning module without causing a tradeoff between regular cell growth and housekeeping protein synthesis. Additionally, we decided to use sfGFP as the reporter gene, instead of a standard mut3b GFP, due to the ability of sfGFP to function even when fused to large bulky protein domains (in this case, FibNT and FibCT). Lastly, we designed BsaI TypeIIS restriction sites flanking the reporter gene that when cut, generates sticky ends compatible with EcoRI and SpeI sites. This was created to allow iGEM teams to take protein domains of interest from the Standard Registry of Parts, and fuse them into the co-spinning module by a simple Digest and Ligate assembly to swap the sfGFP construct. However, the protein domain to be added must have the Silver Fusion prefix and suffix, to allow for in-frame cloning of the protein domain. Without this in-frame cloning, the scar sites of the tradition prefix and suffix will cause a frameshift mutation that mutate the translation product, and renders it non-functional.
mutable provenance: The LacI promoter (BBa_R0010), RBS (BBa_B0034), and super folder GFP (BBa_I746916) were derived from sequences found in the Standard Registry of Parts. The Bombyx mori silk fibroin heavy chain N-terminal (FibNT) and C-terminal (FibCT) domains were derived from the sequenced consensus genome of Bombyx mori silkworm species, and were synthesized using IDT gBlocks gene fragments.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: Elowitz RBS (BBa_B0034)
sequence annotation: BsaI Restriction Site (EcoRI Compatible)
sequence annotation: B. mori FibNT
sequence annotation: B. mori FibCT
sequence annotation: 6x N-terminal polyhistidine tag
sequence annotation: Bba prefix/suffix assembly scar site
sequence annotation: LacI (BBa_R0010)
sequence annotation: Ribosome Double-Stop Site
sequence annotation: superfolder GFP (BBa_I746916)
sequence: https://synbiohub.org/public/igem/BBa_K1763444_sequence/1

Elowitz RBS (BBa_B0034)
type: SequenceAnnotation
persistent identity: annotation2462434
display id: annotation2462434
version: 1
top level: Tamura
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1763444/annotation2462434/range2462434/1

https://synbiohub.org/public/igem/BBa_K1763444/annotation2462434/range2462434/1
type: Range
persistent identity: range2462434
display id: range2462434
version: 1
direction: direction/forward
top level: Tamura
start: 209
end: 220
orientation: inline

BsaI Restriction Site (EcoRI Compatible)
type: SequenceAnnotation
persistent identity: annotation2462437
display id: annotation2462437
version: 1
top level: Tamura
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1763444/annotation2462437/range2462437/1

https://synbiohub.org/public/igem/BBa_K1763444/annotation2462437/range2462437/1
type: Range
persistent identity: range2462437
display id: range2462437
version: 1
direction: direction/forward
top level: Tamura
start: 719
end: 724
orientation: inline

B. mori FibNT
type: SequenceAnnotation
persistent identity: annotation2462436
display id: annotation2462436
version: 1
top level: Tamura
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1763444/annotation2462436/range2462436/1

https://synbiohub.org/public/igem/BBa_K1763444/annotation2462436/range2462436/1
type: Range
persistent identity: range2462436
display id: range2462436
version: 1
direction: direction/forward
top level: Tamura
start: 248
end: 712
orientation: inline

B. mori FibCT
type: SequenceAnnotation
persistent identity: annotation2462439
display id: annotation2462439
version: 1
top level: Tamura
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1763444/annotation2462439/range2462439/1

https://synbiohub.org/public/igem/BBa_K1763444/annotation2462439/range2462439/1
type: Range
persistent identity: range2462439
display id: range2462439
version: 1
direction: direction/forward
top level: Tamura
start: 1451
end: 1627
orientation: inline

6x N-terminal polyhistidine tag
type: SequenceAnnotation
persistent identity: annotation2462435
display id: annotation2462435
version: 1
top level: Tamura
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1763444/annotation2462435/range2462435/1

https://synbiohub.org/public/igem/BBa_K1763444/annotation2462435/range2462435/1
type: Range
persistent identity: range2462435
display id: range2462435
version: 1
direction: direction/forward
top level: Tamura
start: 230
end: 247
orientation: inline

Bba prefix/suffix assembly scar site
type: SequenceAnnotation
persistent identity: annotation2462433
display id: annotation2462433
version: 1
top level: Tamura
role: feature/scar
location: https://synbiohub.org/public/igem/BBa_K1763444/annotation2462433/range2462433/1

https://synbiohub.org/public/igem/BBa_K1763444/annotation2462433/range2462433/1
type: Range
persistent identity: range2462433
display id: range2462433
version: 1
direction: direction/forward
top level: Tamura
start: 201
end: 208
orientation: inline

LacI (BBa_R0010)
type: SequenceAnnotation
persistent identity: annotation2462432
display id: annotation2462432
version: 1
top level: Tamura
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1763444/annotation2462432/range2462432/1

https://synbiohub.org/public/igem/BBa_K1763444/annotation2462432/range2462432/1
type: Range
persistent identity: range2462432
display id: range2462432
version: 1
direction: direction/forward
top level: Tamura
start: 1
end: 200
orientation: inline

Ribosome Double-Stop Site
type: SequenceAnnotation
persistent identity: annotation2462440
display id: annotation2462440
version: 1
top level: Tamura
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1763444/annotation2462440/range2462440/1

https://synbiohub.org/public/igem/BBa_K1763444/annotation2462440/range2462440/1
type: Range
persistent identity: range2462440
display id: range2462440
version: 1
direction: direction/forward
top level: Tamura
start: 1628
end: 1633
orientation: inline

superfolder GFP (BBa_I746916)
type: SequenceAnnotation
persistent identity: annotation2462438
display id: annotation2462438
version: 1
top level: Tamura
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1763444/annotation2462438/range2462438/1

https://synbiohub.org/public/igem/BBa_K1763444/annotation2462438/range2462438/1
type: Range
persistent identity: range2462438
display id: range2462438
version: 1
direction: direction/forward
top level: Tamura
start: 725
end: 1438
orientation: inline