//collections/biofilm
type: Collection
persistent identity: collections biofilm
display id: collections_biofilm
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
owned by: james
owned by: myers
member: DspBx
member: BBa_K1344002
member: BBa_K1510214
member: BBa_K802001
member: BBa_K1701005
member: BBa_K802000
member: BBa_K342003
member: AmyA
member: BBa_K2168001
member: BBa_K1597004
member: BBa_K2168000
member: BBa_K802009

BBa_K802001
type: ComponentDefinition
persistent identity: BBa K802001
display id: BBa_K802001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Dispersin generator for B. subtilis
creator: Remi Hocq, Audrey Masi
created: 2012-09-19T11:00:00Z
modified: 2016-02-12T09:05:08Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1058_
m user id: 4206
owner id: 13266
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: The part contains the DspB gene coding for an enzyme which catalyzes the hydrolysis of the extracellular matrix produced by Gram-negative bacteria.
mutable notes: Used a strong RBS in order to produce high levels of protein.
mutable provenance: Dispersin is produced by Aggregatibacter actinomycetemcomitans.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: Sigma A -10
sequence annotation: BBa_K143021
sequence annotation: rbs
sequence annotation: BBa_K391006
sequence annotation: BBa_B0015
sequence annotation: BBa_K143012
sequence annotation: Sigma A-35
sequence: https://synbiohub.org/public/igem/BBa_K802001_sequence/1

Sigma A -10
type: SequenceAnnotation
persistent identity: annotation2188640
display id: annotation2188640
version: 1
top level: BBa_K802001
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K802001/annotation2188640/range2188640/1

https://synbiohub.org/public/igem/BBa_K802001/annotation2188640/range2188640/1
type: Range
persistent identity: range2188640
display id: range2188640
version: 1
direction: direction/forward
top level: BBa_K802001
start: 86
end: 91
orientation: inline

BBa_K143021
type: SequenceAnnotation
persistent identity: annotation2188643
display id: annotation2188643
version: 1
top level: BBa_K802001
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K802001/annotation2188643/range2188643/1

https://synbiohub.org/public/igem/BBa_K802001/annotation2188643/range2188643/1
type: Range
persistent identity: range2188643
display id: range2188643
version: 1
direction: direction/reverse
top level: BBa_K802001
start: 106
end: 117
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation2188642
display id: annotation2188642
version: 1
top level: BBa_K802001
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K802001/annotation2188642/range2188642/1

https://synbiohub.org/public/igem/BBa_K802001/annotation2188642/range2188642/1
type: Range
persistent identity: range2188642
display id: range2188642
version: 1
direction: direction/forward
top level: BBa_K802001
start: 106
end: 117
orientation: inline

BBa_K391006
type: SequenceAnnotation
persistent identity: annotation2188644
display id: annotation2188644
version: 1
top level: BBa_K802001
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K802001/annotation2188644/range2188644/1

https://synbiohub.org/public/igem/BBa_K802001/annotation2188644/range2188644/1
type: Range
persistent identity: range2188644
display id: range2188644
version: 1
direction: direction/reverse
top level: BBa_K802001
start: 126
end: 1211
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2203894
display id: annotation2203894
version: 1
top level: BBa_K802001
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K802001/annotation2203894/range2203894/1

https://synbiohub.org/public/igem/BBa_K802001/annotation2203894/range2203894/1
type: Range
persistent identity: range2203894
display id: range2203894
version: 1
direction: direction/forward
top level: BBa_K802001
start: 1212
end: 1324
orientation: inline

BBa_K143012
type: SequenceAnnotation
persistent identity: annotation2188641
display id: annotation2188641
version: 1
top level: BBa_K802001
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K802001/annotation2188641/range2188641/1

https://synbiohub.org/public/igem/BBa_K802001/annotation2188641/range2188641/1
type: Range
persistent identity: range2188641
display id: range2188641
version: 1
direction: direction/reverse
top level: BBa_K802001
start: 1
end: 97
orientation: inline

Sigma A-35
type: SequenceAnnotation
persistent identity: annotation2188639
display id: annotation2188639
version: 1
top level: BBa_K802001
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K802001/annotation2188639/range2188639/1

https://synbiohub.org/public/igem/BBa_K802001/annotation2188639/range2188639/1
type: Range
persistent identity: range2188639
display id: range2188639
version: 1
direction: direction/forward
top level: BBa_K802001
start: 63
end: 68
orientation: inline

BBa_K1701005
type: ComponentDefinition
persistent identity: BBa K1701005
display id: BBa_K1701005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TasA
creator: Wei Wei
created: 2015-09-07T11:00:00Z
modified: 2016-02-12T09:11:55Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _2119_
m user id: 4206
owner id: 21319
owning group id: 9
status: status/Planning
bookmark: false
mutable description: In B. subtilis the extracellular matrix is composed of two major components, an EPS and the protein TasA, which polymerizes into amyloid-like fibers.
mutable notes: None
mutable provenance: http://www.ncbi.nlm.nih.gov/nuccore/56068618
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1701005_sequence/1

DspBx
type: ComponentDefinition
persistent identity: BBa K1659210
display id: BBa_K1659210
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Dispersin B with mutation at nucleotide 582 (T to C)
creator: Wei Chung Kong
created: 2015-08-27T11:00:00Z
modified: 2016-02-12T09:06:47Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2077_
m user id: 4206
owner id: 24524
owning group id: 9
status: status/Planning
bookmark: false
mutable description: to be updated
mutable notes: to be updated
mutable provenance: to be updated
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Hisx6
sequence annotation: DspBx
sequence annotation: stop
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_K1659210_sequence/1

Hisx6
type: SequenceAnnotation
persistent identity: annotation2449119
display id: annotation2449119
version: 1
top level: DspBx
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1659210/annotation2449119/range2449119/1

https://synbiohub.org/public/igem/BBa_K1659210/annotation2449119/range2449119/1
type: Range
persistent identity: range2449119
display id: range2449119
version: 1
direction: direction/forward
top level: DspBx
start: 1087
end: 1104
orientation: inline

DspBx
type: SequenceAnnotation
persistent identity: annotation2449118
display id: annotation2449118
version: 1
top level: DspBx
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1659210/annotation2449118/range2449118/1

https://synbiohub.org/public/igem/BBa_K1659210/annotation2449118/range2449118/1
type: Range
persistent identity: range2449118
display id: range2449118
version: 1
direction: direction/forward
top level: DspBx
start: 4
end: 1086
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2453187
display id: annotation2453187
version: 1
top level: DspBx
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1659210/annotation2453187/range2453187/1

https://synbiohub.org/public/igem/BBa_K1659210/annotation2453187/range2453187/1
type: Range
persistent identity: range2453187
display id: range2453187
version: 1
direction: direction/forward
top level: DspBx
start: 1105
end: 1110
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2449117
display id: annotation2449117
version: 1
top level: DspBx
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1659210/annotation2449117/range2449117/1

https://synbiohub.org/public/igem/BBa_K1659210/annotation2449117/range2449117/1
type: Range
persistent identity: range2449117
display id: range2449117
version: 1
direction: direction/forward
top level: DspBx
start: 1
end: 3
orientation: inline

BBa_K802000
type: ComponentDefinition
persistent identity: BBa K802000
display id: BBa_K802000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Lysostaphin generator for B. subtilis
creator: Carine Gimbert, Anne Haziza
created: 2012-09-19T11:00:00Z
modified: 2016-02-12T09:52:54Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1058_
m user id: 4206
owner id: 13266
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: Lysostaphin is a bacterial biocide isolated from Staphylococcus simulans.
mutable notes: Used a strong RBS in order to produce high levels of expression of Lysostaphin.
mutable provenance: This is a composite part including the constitutive B. subtilis Pveg promotor and lysostaphin coding sequence.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: Sigma A-35
sequence annotation: BBa_K143021
sequence annotation: Sigma A -10
sequence annotation: rbs
sequence annotation: BBa_K143012
sequence: https://synbiohub.org/public/igem/BBa_K802000_sequence/1

Sigma A-35
type: SequenceAnnotation
persistent identity: annotation2188634
display id: annotation2188634
version: 1
top level: BBa_K802000
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K802000/annotation2188634/range2188634/1

https://synbiohub.org/public/igem/BBa_K802000/annotation2188634/range2188634/1
type: Range
persistent identity: range2188634
display id: range2188634
version: 1
direction: direction/forward
top level: BBa_K802000
start: 63
end: 68
orientation: inline

BBa_K143021
type: SequenceAnnotation
persistent identity: annotation2188638
display id: annotation2188638
version: 1
top level: BBa_K802000
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K802000/annotation2188638/range2188638/1

https://synbiohub.org/public/igem/BBa_K802000/annotation2188638/range2188638/1
type: Range
persistent identity: range2188638
display id: range2188638
version: 1
direction: direction/reverse
top level: BBa_K802000
start: 106
end: 117
orientation: inline

Sigma A -10
type: SequenceAnnotation
persistent identity: annotation2188635
display id: annotation2188635
version: 1
top level: BBa_K802000
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K802000/annotation2188635/range2188635/1

https://synbiohub.org/public/igem/BBa_K802000/annotation2188635/range2188635/1
type: Range
persistent identity: range2188635
display id: range2188635
version: 1
direction: direction/forward
top level: BBa_K802000
start: 86
end: 91
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation2188637
display id: annotation2188637
version: 1
top level: BBa_K802000
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K802000/annotation2188637/range2188637/1

https://synbiohub.org/public/igem/BBa_K802000/annotation2188637/range2188637/1
type: Range
persistent identity: range2188637
display id: range2188637
version: 1
direction: direction/forward
top level: BBa_K802000
start: 106
end: 117
orientation: inline

BBa_K143012
type: SequenceAnnotation
persistent identity: annotation2188636
display id: annotation2188636
version: 1
top level: BBa_K802000
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K802000/annotation2188636/range2188636/1

https://synbiohub.org/public/igem/BBa_K802000/annotation2188636/range2188636/1
type: Range
persistent identity: range2188636
display id: range2188636
version: 1
direction: direction/reverse
top level: BBa_K802000
start: 1
end: 97
orientation: inline

BBa_K2168000
type: ComponentDefinition
persistent identity: BBa K2168000
display id: BBa_K2168000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pBAD/araC - N(6xHis) Ag43
creator: Arunavo Chakraborty
created: 2016-10-12T11:00:00Z
modified: 2016-10-13T02:44:12Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2639_
m user id: 30486
owner id: 30486
owning group id: 9
status: status/Planning
bookmark: true
mutable description: This part encodes E coli protein Ag43, with an additional 6xHis tag for easy purification and quantification. Ag43 expression is known to cause E coli cells to adhere together, and the 6xHis tag was added to allow for convenient study of variation of E coli aggregation with respect to Ag43 expression levels.
mutable notes: Ag43 is an outer membrane E coli protein, so it has a N-terminal 52 amino acid long signal peptide sequence, which is cleaved after Ag43 pro-protein is transported into the periplasmic space. Since strong dependence has been demonstrated on the signal peptide cleavage on the signal peptide-protein junction, the 6xHis tag should be added after Ala53, the residue immediately after the signal peptide sequence.
mutable provenance: This comes from Gibson assembly of fragments from a previous biobrick, BBa_K1352000 by Team:Aberdeen_Scotland, 2014.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Double terminator (B0015)
sequence annotation: 6xHis tag
sequence annotation: pBAD
sequence annotation: araC
sequence annotation: B0034
sequence annotation: Ag43(PstI sites removed)
sequence: https://synbiohub.org/public/igem/BBa_K2168000_sequence/1

Double terminator (B0015)
type: SequenceAnnotation
persistent identity: annotation2499426
display id: annotation2499426
version: 1
top level: BBa_K2168000
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K2168000/annotation2499426/range2499426/1

https://synbiohub.org/public/igem/BBa_K2168000/annotation2499426/range2499426/1
type: Range
persistent identity: range2499426
display id: range2499426
version: 1
direction: direction/forward
top level: BBa_K2168000
start: 4383
end: 4511
orientation: inline

6xHis tag
type: SequenceAnnotation
persistent identity: annotation2499423
display id: annotation2499423
version: 1
top level: BBa_K2168000
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K2168000/annotation2499423/range2499423/1

https://synbiohub.org/public/igem/BBa_K2168000/annotation2499423/range2499423/1
type: Range
persistent identity: range2499423
display id: range2499423
version: 1
direction: direction/forward
top level: BBa_K2168000
start: 1396
end: 1413
orientation: inline

pBAD
type: SequenceAnnotation
persistent identity: annotation2499411
display id: annotation2499411
version: 1
top level: BBa_K2168000
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K2168000/annotation2499411/range2499411/1

https://synbiohub.org/public/igem/BBa_K2168000/annotation2499411/range2499411/1
type: Range
persistent identity: range2499411
display id: range2499411
version: 1
direction: direction/forward
top level: BBa_K2168000
start: 906
end: 1190
orientation: inline

araC
type: SequenceAnnotation
persistent identity: annotation2499395
display id: annotation2499395
version: 1
top level: BBa_K2168000
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K2168000/annotation2499395/range2499395/1

https://synbiohub.org/public/igem/BBa_K2168000/annotation2499395/range2499395/1
type: Range
persistent identity: range2499395
display id: range2499395
version: 1
direction: direction/reverse
top level: BBa_K2168000
start: 1
end: 879
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation2499416
display id: annotation2499416
version: 1
top level: BBa_K2168000
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K2168000/annotation2499416/range2499416/1

https://synbiohub.org/public/igem/BBa_K2168000/annotation2499416/range2499416/1
type: Range
persistent identity: range2499416
display id: range2499416
version: 1
direction: direction/forward
top level: BBa_K2168000
start: 1219
end: 1230
orientation: inline

Ag43(PstI sites removed)
type: SequenceAnnotation
persistent identity: annotation2499418
display id: annotation2499418
version: 1
top level: BBa_K2168000
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K2168000/annotation2499418/range2499418/1

https://synbiohub.org/public/igem/BBa_K2168000/annotation2499418/range2499418/1
type: Range
persistent identity: range2499418
display id: range2499418
version: 1
direction: direction/forward
top level: BBa_K2168000
start: 1237
end: 4374
orientation: inline

BBa_K1597004
type: ComponentDefinition
persistent identity: BBa K1597004
display id: BBa_K1597004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: slrR with strong promoter and RBS
creator: Marieke Mulder
created: 2015-09-13T11:00:00Z
modified: 2016-02-12T09:10:03Z
discontinued: false
dominant: false
group u list: _2014_
m user id: 4206
owner id: 24529
owning group id: 9
status: status/Planning
bookmark: false
mutable description: SlrR is an important transcriptional regulator in Bacillus subtilis. SlrR represses the sigma(D)-dependent flagellar genes and activates the eps and yqxM operons. Therefore it is has a controlling factor in biofilm formation. By overexpressing SlrR, with the use of the promoter Pveg and a strong RBS, a robust biofilm can be formed.
mutable notes: This gene was ordered via IDT, hence it was not biobrick compatible.
mutable provenance: Ordered via IDT based on the genomic sequence from Bacillus subtilis 168
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: putative ComK binding region
sequence annotation: BBa_K780001
sequence annotation: slrR
sequence annotation: BBa_K090504
sequence annotation: putative SigA -10
sequence annotation: putative SigA -35
sequence: https://synbiohub.org/public/igem/BBa_K1597004_sequence/1

putative ComK binding region
type: SequenceAnnotation
persistent identity: annotation2454115
display id: annotation2454115
version: 1
top level: BBa_K1597004
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K1597004/annotation2454115/range2454115/1

https://synbiohub.org/public/igem/BBa_K1597004/annotation2454115/range2454115/1
type: Range
persistent identity: range2454115
display id: range2454115
version: 1
direction: direction/forward
top level: BBa_K1597004
start: 76
end: 88
orientation: inline

BBa_K780001
type: SequenceAnnotation
persistent identity: annotation2454119
display id: annotation2454119
version: 1
top level: BBa_K1597004
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1597004/annotation2454119/range2454119/1

https://synbiohub.org/public/igem/BBa_K1597004/annotation2454119/range2454119/1
type: Range
persistent identity: range2454119
display id: range2454119
version: 1
direction: direction/forward
top level: BBa_K1597004
start: 258
end: 273
orientation: inline

slrR
type: SequenceAnnotation
persistent identity: annotation2454137
display id: annotation2454137
version: 1
top level: BBa_K1597004
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1597004/annotation2454137/range2454137/1

https://synbiohub.org/public/igem/BBa_K1597004/annotation2454137/range2454137/1
type: Range
persistent identity: range2454137
display id: range2454137
version: 1
direction: direction/forward
top level: BBa_K1597004
start: 278
end: 751
orientation: inline

BBa_K090504
type: SequenceAnnotation
persistent identity: annotation2454118
display id: annotation2454118
version: 1
top level: BBa_K1597004
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1597004/annotation2454118/range2454118/1

https://synbiohub.org/public/igem/BBa_K1597004/annotation2454118/range2454118/1
type: Range
persistent identity: range2454118
display id: range2454118
version: 1
direction: direction/forward
top level: BBa_K1597004
start: 1
end: 239
orientation: inline

putative SigA -10
type: SequenceAnnotation
persistent identity: annotation2454117
display id: annotation2454117
version: 1
top level: BBa_K1597004
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1597004/annotation2454117/range2454117/1

https://synbiohub.org/public/igem/BBa_K1597004/annotation2454117/range2454117/1
type: Range
persistent identity: range2454117
display id: range2454117
version: 1
direction: direction/forward
top level: BBa_K1597004
start: 125
end: 130
orientation: inline

putative SigA -35
type: SequenceAnnotation
persistent identity: annotation2454116
display id: annotation2454116
version: 1
top level: BBa_K1597004
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1597004/annotation2454116/range2454116/1

https://synbiohub.org/public/igem/BBa_K1597004/annotation2454116/range2454116/1
type: Range
persistent identity: range2454116
display id: range2454116
version: 1
direction: direction/forward
top level: BBa_K1597004
start: 102
end: 108
orientation: inline

BBa_K1344002
type: ComponentDefinition
persistent identity: BBa K1344002
display id: BBa_K1344002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Nuc-HlyA
creator: UI Indonesia 2014
created: 2014-10-03T11:00:00Z
modified: 2016-02-12T09:03:47Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _1719_
m user id: 4206
owner id: 20150
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part is based on BBa_K729004, modified by deleting the stop codons. It's also fusion with HlyA secretional tag for extracelullar expression
mutable notes: This part makes the Nuclease secreted extracelullar
mutable provenance: It was modified from BBa_K729004, BBa_K554002
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Nuclease
sequence annotation: HlyA
sequence annotation: Stop
sequence: https://synbiohub.org/public/igem/BBa_K1344002_sequence/1

Nuclease
type: SequenceAnnotation
persistent identity: annotation2393930
display id: annotation2393930
version: 1
top level: BBa_K1344002
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1344002/annotation2393930/range2393930/1

https://synbiohub.org/public/igem/BBa_K1344002/annotation2393930/range2393930/1
type: Range
persistent identity: range2393930
display id: range2393930
version: 1
direction: direction/forward
top level: BBa_K1344002
start: 1
end: 558
orientation: inline

HlyA
type: SequenceAnnotation
persistent identity: annotation2393931
display id: annotation2393931
version: 1
top level: BBa_K1344002
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1344002/annotation2393931/range2393931/1

https://synbiohub.org/public/igem/BBa_K1344002/annotation2393931/range2393931/1
type: Range
persistent identity: range2393931
display id: range2393931
version: 1
direction: direction/forward
top level: BBa_K1344002
start: 567
end: 746
orientation: inline

Stop
type: SequenceAnnotation
persistent identity: annotation2393932
display id: annotation2393932
version: 1
top level: BBa_K1344002
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1344002/annotation2393932/range2393932/1

https://synbiohub.org/public/igem/BBa_K1344002/annotation2393932/range2393932/1
type: Range
persistent identity: range2393932
display id: range2393932
version: 1
direction: direction/forward
top level: BBa_K1344002
start: 747
end: 752
orientation: inline

BBa_K342003
type: ComponentDefinition
persistent identity: BBa K342003
display id: BBa_K342003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: OmpR234 protein, with higher effect on Curli promoter
creator: Nathalie Isorce
created: 2010-10-23T11:00:00Z
modified: 2016-02-12T09:01:32Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _471_
m user id: 4206
owner id: 6842
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This part is coding for a mutated protein OmpR. This protein will be phosphorilated by the complex Envz. The phosphorilated protein is an activator of the Curli promoter
mutable notes: enter
mutable provenance: pcr
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K342003_sequence/1

BBa_K1510214
type: ComponentDefinition
persistent identity: BBa K1510214
display id: BBa_K1510214
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Dispersin generator circuit
creator: Kao Jung Chang
created: 2014-10-07T11:00:00Z
modified: 2016-02-12T09:00:45Z
discontinued: false
dominant: false
group u list: _1890_
m user id: 4206
owner id: 21271
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: he Dispersin gene codes for an enzyme which catalyzes the hydrolysis of the extracellular matrix produced by Gram negative bacteria.With strong constitutive promoter,together with ribosomal binding site and signal sequence yebF, make dispersin work more accurately.
mutable notes: To fit igem part standard, the circuit is constructed in backbone pSB1C3, chloramphenicol with EcoR1 and Xba1 in front of the part, and Spe1, Pst1 behind the part.
mutable provenance: The promoter, J23100, comes from the 2014 distributed igem kit. The signal sequence, yebF, encoding signal protein exporting recombinant protein extracellular, is from E.coli K-12. By designing primer, we obtain it together with its naturally occurring ribosomal binding site using PCR and extraction.The dispersin is from Lyon INSA igem team. Finally, the terminator B0015 is from 2014 igem distribution.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Conjugation
role: SO:0000724
component: BBa_J23100
component: BBa_K1510009
component: BBa_B0015
component: BBa_K802001
sequence annotation: BBa_K802001
sequence annotation: BBa_B0015
sequence annotation: BBa_J23100
sequence annotation: BBa_K1510009
sequence: https://synbiohub.org/public/igem/BBa_K1510214_sequence/1

BBa_J23100
type: Component
persistent identity: component2404698
display id: component2404698
version: 1
top level: BBa_K1510214
access: public
definition: https://synbiohub.org/public/igem/BBa_J23100/1

BBa_K1510009
type: Component
persistent identity: component2404699
display id: component2404699
version: 1
top level: BBa_K1510214
access: public
definition: https://synbiohub.org/public/igem/BBa_K1510009/1

BBa_B0015
type: Component
persistent identity: component2404706
display id: component2404706
version: 1
top level: BBa_K1510214
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_B0015
type: Component
persistent identity: component2404714
display id: component2404714
version: 1
top level: BBa_K1510214
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_K802001
type: Component
persistent identity: component2404707
display id: component2404707
version: 1
top level: BBa_K1510214
access: public
definition: BBa_K802001

BBa_K802001
type: SequenceAnnotation
persistent identity: annotation2404707
display id: annotation2404707
version: 1
top level: BBa_K1510214
location: https://synbiohub.org/public/igem/BBa_K1510214/annotation2404707/range2404707/1
component: BBa_K802001

https://synbiohub.org/public/igem/BBa_K1510214/annotation2404707/range2404707/1
type: Range
persistent identity: range2404707
display id: range2404707
version: 1
direction: direction/reverse
top level: BBa_K1510214
start: 593
end: 1916
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2404714
display id: annotation2404714
version: 1
top level: BBa_K1510214
location: https://synbiohub.org/public/igem/BBa_K1510214/annotation2404714/range2404714/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K1510214/annotation2404714/range2404714/1
type: Range
persistent identity: range2404714
display id: range2404714
version: 1
direction: direction/reverse
top level: BBa_K1510214
start: 1925
end: 2053
orientation: inline

BBa_J23100
type: SequenceAnnotation
persistent identity: annotation2404698
display id: annotation2404698
version: 1
top level: BBa_K1510214
location: https://synbiohub.org/public/igem/BBa_K1510214/annotation2404698/range2404698/1
component: BBa_J23100

https://synbiohub.org/public/igem/BBa_K1510214/annotation2404698/range2404698/1
type: Range
persistent identity: range2404698
display id: range2404698
version: 1
direction: direction/reverse
top level: BBa_K1510214
start: 1
end: 35
orientation: inline

BBa_K1510009
type: SequenceAnnotation
persistent identity: annotation2404699
display id: annotation2404699
version: 1
top level: BBa_K1510214
location: https://synbiohub.org/public/igem/BBa_K1510214/annotation2404699/range2404699/1
component: BBa_K1510009

https://synbiohub.org/public/igem/BBa_K1510214/annotation2404699/range2404699/1
type: Range
persistent identity: range2404699
display id: range2404699
version: 1
direction: direction/reverse
top level: BBa_K1510214
start: 44
end: 584
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2404706
display id: annotation2404706
version: 1
top level: BBa_K1510214
location: https://synbiohub.org/public/igem/BBa_K1510214/annotation2404706/range2404706/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K1510214/annotation2404706/range2404706/1
type: Range
persistent identity: range2404706
display id: range2404706
version: 1
direction: direction/forward
top level: BBa_K1510214
start: 1804
end: 1916
orientation: inline

BBa_K2168001
type: ComponentDefinition
persistent identity: BBa K2168001
display id: BBa_K2168001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pBAD/araC - (N-6xHis) - Ag43 - sfGFP
creator: Arunavo Chakraborty
created: 2016-10-12T11:00:00Z
modified: 2016-10-13T03:15:53Z
discontinued: false
dominant: false
group u list: _2639_
m user id: 30486
owner id: 30486
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This is a fusion protein with Ag43, a protein from E coli tagged with a 6xHis tag on the N-terminal and sfGFP tagged on C-terminal of the pro-protein. Ag43 causes cell-cell adhesion in E coli, and the 6xHis and sfGFP tags are present to allow convenient study dependence of Ag43 mediated aggregation on Ag43 expression levels, which can be determined from the sfGFP fluoresence and/or quantification of 6xHis tag. The 6xHis tag is included to allow protein concentration estimation in case the sfGFP fails to fold properly.
mutable notes: The location for the 6xHis tag is the same as that for BBa_K2168000(Ag43 tagged with 6xHis on N-terminal under pBAD/araC promoter) and uses the same design principles. For independent folding, a flexible GGGGSGGGGS linker was added between Ag43 and sfGFP, and the linker was choosen to be not very hydrophilic or hydrophobic, since Ag43 lies very close to lipid membranes.
mutable provenance: This part was made from Gibson assemblies from fragments of two previous parts - BBa_K1352000 and BBa_I746908, which code for Ag43 and sfGFP respectively.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Double terminator (B0015)
sequence annotation: 6xHis
sequence annotation: Ag43(PstI sites removed)
sequence annotation: araC
sequence annotation: (GGGGS)2 linker
sequence annotation: pBAD
sequence annotation: B0034
sequence annotation: sfGFP
sequence: https://synbiohub.org/public/igem/BBa_K2168001_sequence/1

Double terminator (B0015)
type: SequenceAnnotation
persistent identity: annotation2499489
display id: annotation2499489
version: 1
top level: BBa_K2168001
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K2168001/annotation2499489/range2499489/1

https://synbiohub.org/public/igem/BBa_K2168001/annotation2499489/range2499489/1
type: Range
persistent identity: range2499489
display id: range2499489
version: 1
direction: direction/forward
top level: BBa_K2168001
start: 5130
end: 5258
orientation: inline

6xHis
type: SequenceAnnotation
persistent identity: annotation2499490
display id: annotation2499490
version: 1
top level: BBa_K2168001
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K2168001/annotation2499490/range2499490/1

https://synbiohub.org/public/igem/BBa_K2168001/annotation2499490/range2499490/1
type: Range
persistent identity: range2499490
display id: range2499490
version: 1
direction: direction/forward
top level: BBa_K2168001
start: 1396
end: 1413
orientation: inline

Ag43(PstI sites removed)
type: SequenceAnnotation
persistent identity: annotation2499486
display id: annotation2499486
version: 1
top level: BBa_K2168001
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K2168001/annotation2499486/range2499486/1

https://synbiohub.org/public/igem/BBa_K2168001/annotation2499486/range2499486/1
type: Range
persistent identity: range2499486
display id: range2499486
version: 1
direction: direction/forward
top level: BBa_K2168001
start: 1237
end: 4371
orientation: inline

araC
type: SequenceAnnotation
persistent identity: annotation2499465
display id: annotation2499465
version: 1
top level: BBa_K2168001
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K2168001/annotation2499465/range2499465/1

https://synbiohub.org/public/igem/BBa_K2168001/annotation2499465/range2499465/1
type: Range
persistent identity: range2499465
display id: range2499465
version: 1
direction: direction/reverse
top level: BBa_K2168001
start: 1
end: 879
orientation: inline

(GGGGS)2 linker
type: SequenceAnnotation
persistent identity: annotation2499487
display id: annotation2499487
version: 1
top level: BBa_K2168001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K2168001/annotation2499487/range2499487/1

https://synbiohub.org/public/igem/BBa_K2168001/annotation2499487/range2499487/1
type: Range
persistent identity: range2499487
display id: range2499487
version: 1
direction: direction/forward
top level: BBa_K2168001
start: 4372
end: 4401
orientation: inline

pBAD
type: SequenceAnnotation
persistent identity: annotation2499484
display id: annotation2499484
version: 1
top level: BBa_K2168001
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K2168001/annotation2499484/range2499484/1

https://synbiohub.org/public/igem/BBa_K2168001/annotation2499484/range2499484/1
type: Range
persistent identity: range2499484
display id: range2499484
version: 1
direction: direction/forward
top level: BBa_K2168001
start: 906
end: 1190
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation2499485
display id: annotation2499485
version: 1
top level: BBa_K2168001
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K2168001/annotation2499485/range2499485/1

https://synbiohub.org/public/igem/BBa_K2168001/annotation2499485/range2499485/1
type: Range
persistent identity: range2499485
display id: range2499485
version: 1
direction: direction/forward
top level: BBa_K2168001
start: 1219
end: 1230
orientation: inline

sfGFP
type: SequenceAnnotation
persistent identity: annotation2499488
display id: annotation2499488
version: 1
top level: BBa_K2168001
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K2168001/annotation2499488/range2499488/1

https://synbiohub.org/public/igem/BBa_K2168001/annotation2499488/range2499488/1
type: Range
persistent identity: range2499488
display id: range2499488
version: 1
direction: direction/forward
top level: BBa_K2168001
start: 4402
end: 5118
orientation: inline

BBa_K802009
type: ComponentDefinition
persistent identity: BBa K802009
display id: BBa_K802009
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Sufactin generator and biofilm repressor for B. subtilis
creator: Ioana Sandu, Gael Chambonnier, Alex Mizgier, Marion Traouan
created: 2012-09-24T11:00:00Z
modified: 2016-02-12T09:05:33Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1058_
m user id: 4206
owner id: 13266
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part can be used to induce surfactin production (an antimicrobial lipopeptide) and to repress the biofilm formation in B. subtilis strains.
mutable notes: The part has 3 genes (sfp, abrB and lacI) under the control of the xylose inducible Pxyl promoter.
mutable provenance: The part was constructed from the assembly of 3 biobricks: BBa_K802006,BBa_K802007 and BBa_K802008.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_K802006
component: BBa_K802007
component: BBa_K802008
sequence annotation: BBa_K802007
sequence annotation: BBa_K802008
sequence annotation: BBa_K802006
sequence: https://synbiohub.org/public/igem/BBa_K802009_sequence/1

BBa_K802006
type: Component
persistent identity: component2198693
display id: component2198693
version: 1
top level: BBa_K802009
access: public
definition: https://synbiohub.org/public/igem/BBa_K802006/1

BBa_K802007
type: Component
persistent identity: component2198694
display id: component2198694
version: 1
top level: BBa_K802009
access: public
definition: https://synbiohub.org/public/igem/BBa_K802007/1

BBa_K802008
type: Component
persistent identity: component2198695
display id: component2198695
version: 1
top level: BBa_K802009
access: public
definition: https://synbiohub.org/public/igem/BBa_K802008/1

BBa_K802007
type: SequenceAnnotation
persistent identity: annotation2198694
display id: annotation2198694
version: 1
top level: BBa_K802009
location: https://synbiohub.org/public/igem/BBa_K802009/annotation2198694/range2198694/1
component: BBa_K802007

https://synbiohub.org/public/igem/BBa_K802009/annotation2198694/range2198694/1
type: Range
persistent identity: range2198694
display id: range2198694
version: 1
direction: direction/reverse
top level: BBa_K802009
start: 796
end: 1099
orientation: inline

BBa_K802008
type: SequenceAnnotation
persistent identity: annotation2198695
display id: annotation2198695
version: 1
top level: BBa_K802009
location: https://synbiohub.org/public/igem/BBa_K802009/annotation2198695/range2198695/1
component: BBa_K802008

https://synbiohub.org/public/igem/BBa_K802009/annotation2198695/range2198695/1
type: Range
persistent identity: range2198695
display id: range2198695
version: 1
direction: direction/reverse
top level: BBa_K802009
start: 1108
end: 2300
orientation: inline

BBa_K802006
type: SequenceAnnotation
persistent identity: annotation2198693
display id: annotation2198693
version: 1
top level: BBa_K802009
location: https://synbiohub.org/public/igem/BBa_K802009/annotation2198693/range2198693/1
component: BBa_K802006

https://synbiohub.org/public/igem/BBa_K802009/annotation2198693/range2198693/1
type: Range
persistent identity: range2198693
display id: range2198693
version: 1
direction: direction/reverse
top level: BBa_K802009
start: 1
end: 787
orientation: inline

AmyA
type: ComponentDefinition
persistent identity: BBa K1195001
display id: BBa_K1195001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Amylase A
creator: Clarice Harrison
created: 2013-08-27T11:00:00Z
modified: 2016-02-12T09:08:04Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1509_
m user id: 4206
owner id: 17504
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: A enzyme that is know to degrade biofilms.
mutable notes: None.
mutable provenance: ?????
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_K1195001_sequence/1