//collections/antifreeze
type: Collection
persistent identity: collections antifreeze
display id: collections_antifreeze
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
owned by: james
owned by: myers
member: BBa_M36996
member: BBa_K1809000
member: BBa_K1809018
member: BBa_K1809021
member: BBa_K1809027
member: BBa_K729016
member: BBa_K1809006
member: BBa_K1809031
member: BBa_K1831003
member: BBa_K942011
member: BBa_K1809009
member: BBa_K1809029
member: BBa_M36951
member: BBa_K1809011
member: BBa_K1809003
member: BBa_M36999
member: BBa_K1809025
member: BBa_M36950
member: BBa_K1809023
member: BBa_K729018
member: BBa_K1809036
member: BBa_K1809012
member: BBa_M36997
member: BBa_K1809015
member: AFP
member: BBa_K1809033
member: UNIK Antif
member: BBa_K1831001
member: BBa_K1809037

BBa_K942011
type: ComponentDefinition
persistent identity: BBa K942011
display id: BBa_K942011
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RiAFP+His
creator: Luis Mario Leal Garza
created: 2012-09-25T11:00:00Z
modified: 2016-01-27T10:45:02Z
discontinued: false
dominant: false
group u list: _1209_
m user id: 4206
owner id: 13053
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: The Raghium inquisitor Anti Freeze Protein (RiAFP) is a 137 AA protein that prevents the growth of ice crystals. This protein is produced natively in the beetle Raghium inquisitor. (Kristiansen E, 2011) About Safety The RiAFP has no reported toxicity or hazardousness, but its important to remember to have the basic biosafety precautions using a biosecurity chamber when handling any transformed strains.
mutable notes: This part was designed by adding a 6xHis tag for affinity purification to the anti freeze protein from Raghium inquisitor. This part was codon optimized for its expression in E.coli.
mutable provenance: Is produced natively in the beetle Raghium inquisitor. (Kristiansen E, 2011)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K942011_sequence/1

BBa_K1809037
type: ComponentDefinition
persistent identity: BBa K1809037
display id: BBa_K1809037
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PpAFP
creator: David Morgan
created: 2015-09-02T11:00:00Z
modified: 2016-01-27T10:39:28Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2234_
m user id: 4206
owner id: 24831
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Antifreeze proteins (AFPs) are molecules with the unique property of binding and shaping ice crystals, preventing their growth into relatively large cell-lysing structures (Davies and Sykes 1997). They exhibit a property known as thermal hysteresis, whereby their activity decreases the freezing point of water without changing the melting point. This property allows organisms which produce AFPs to survive colder conditions. Additionally, AFPs have shown biolfilm inhibiting properties, potentially giving them uses in medical applications.
mutable notes: Codon optimized the sequence for E. coli and to minimize repetitive sequences; removed illegal restriction sites.
mutable provenance: Taken from the rhizobacterium Pseudemonas putida GR12-2. Pubmed ID: 15317770
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1809037_sequence/1

BBa_K1809029
type: ComponentDefinition
persistent identity: BBa K1809029
display id: BBa_K1809029
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: MoAFP
creator: David Morgan
created: 2015-08-25T11:00:00Z
modified: 2016-01-27T10:36:48Z
discontinued: false
dominant: false
group u list: _2234_
m user id: 4206
owner id: 24831
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Antifreeze proteins (AFPs) are molecules with the unique property of binding and shaping ice crystals, preventing their growth into relatively large cell-lysing structures (Davies and Sykes 1997). They exhibit a property known as thermal hysteresis, whereby their activity decreases the freezing point of water without changing the melting point. This property allows organisms which produce AFPs to survive colder conditions. Additionally, AFPs have shown biolfilm inhibiting properties, potentially giving them uses in medical applications.
mutable notes: Codon optimized the sequence for E. coli and to minimize repetitive sequences; removed illegal restriction sites.
mutable provenance: Taken from the longhorn sculpin Myoxocephalus octodecemspinosus. Pubmed ID: 11136728
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1809029_sequence/1

BBa_M36999
type: ComponentDefinition
persistent identity: BBa M36999
display id: BBa_M36999
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Non-Glycolated Antifreeze Protein (AFP) - 5-Repeated Segment
creator: Nathan Barnett, Meghan Bowler, Kian Torabian
created: 2011-04-27T11:00:00Z
modified: 2016-01-27T10:45:33Z
discontinued: false
dominant: false
experience: experience/None
group u list: _848_
m user id: 4206
owner id: 9543
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: The standard non-glycolated antifreeze protein (AFP) from the Pseudopleuronecta americanus. Begins with a chain of C's and is followed by 5 rough repeats of "ANAAAAAALTA". The codons have been optimized for E. coli.
mutable notes: This specific sequence has the repeat 5 times. Studies show that increasing the number of repeats increases the antifreeze capabilities of the protein.
mutable provenance: Comes from the white flounder, or Pseudopleuronecta americanus.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Unit 1 (TAANAAAAAAL)
sequence annotation: start
sequence annotation: C-linker
sequence annotation: stop
sequence annotation: Unit 4 (TAANAAAAAAL)
sequence annotation: Modified Unit 0 (TASDAAAAAL)
sequence annotation: Unit 3 (TAANAAAAAAL)
sequence annotation: Unit 2 (TAANAAAAAAL)
sequence: https://synbiohub.org/public/igem/BBa_M36999_sequence/1

Unit 1 (TAANAAAAAAL)
type: SequenceAnnotation
persistent identity: annotation2119820
display id: annotation2119820
version: 1
top level: BBa_M36999
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_M36999/annotation2119820/range2119820/1

https://synbiohub.org/public/igem/BBa_M36999/annotation2119820/range2119820/1
type: Range
persistent identity: range2119820
display id: range2119820
version: 1
direction: direction/forward
top level: BBa_M36999
start: 46
end: 78
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2119818
display id: annotation2119818
version: 1
top level: BBa_M36999
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_M36999/annotation2119818/range2119818/1

https://synbiohub.org/public/igem/BBa_M36999/annotation2119818/range2119818/1
type: Range
persistent identity: range2119818
display id: range2119818
version: 1
direction: direction/forward
top level: BBa_M36999
start: 10
end: 12
orientation: inline

C-linker
type: SequenceAnnotation
persistent identity: annotation2119817
display id: annotation2119817
version: 1
top level: BBa_M36999
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_M36999/annotation2119817/range2119817/1

https://synbiohub.org/public/igem/BBa_M36999/annotation2119817/range2119817/1
type: Range
persistent identity: range2119817
display id: range2119817
version: 1
direction: direction/forward
top level: BBa_M36999
start: 1
end: 9
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2119824
display id: annotation2119824
version: 1
top level: BBa_M36999
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_M36999/annotation2119824/range2119824/1

https://synbiohub.org/public/igem/BBa_M36999/annotation2119824/range2119824/1
type: Range
persistent identity: range2119824
display id: range2119824
version: 1
direction: direction/forward
top level: BBa_M36999
start: 187
end: 192
orientation: inline

Unit 4 (TAANAAAAAAL)
type: SequenceAnnotation
persistent identity: annotation2119823
display id: annotation2119823
version: 1
top level: BBa_M36999
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_M36999/annotation2119823/range2119823/1

https://synbiohub.org/public/igem/BBa_M36999/annotation2119823/range2119823/1
type: Range
persistent identity: range2119823
display id: range2119823
version: 1
direction: direction/forward
top level: BBa_M36999
start: 145
end: 177
orientation: inline

Modified Unit 0 (TASDAAAAAL)
type: SequenceAnnotation
persistent identity: annotation2119819
display id: annotation2119819
version: 1
top level: BBa_M36999
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_M36999/annotation2119819/range2119819/1

https://synbiohub.org/public/igem/BBa_M36999/annotation2119819/range2119819/1
type: Range
persistent identity: range2119819
display id: range2119819
version: 1
direction: direction/forward
top level: BBa_M36999
start: 16
end: 45
orientation: inline

Unit 3 (TAANAAAAAAL)
type: SequenceAnnotation
persistent identity: annotation2119822
display id: annotation2119822
version: 1
top level: BBa_M36999
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_M36999/annotation2119822/range2119822/1

https://synbiohub.org/public/igem/BBa_M36999/annotation2119822/range2119822/1
type: Range
persistent identity: range2119822
display id: range2119822
version: 1
direction: direction/forward
top level: BBa_M36999
start: 112
end: 144
orientation: inline

Unit 2 (TAANAAAAAAL)
type: SequenceAnnotation
persistent identity: annotation2119821
display id: annotation2119821
version: 1
top level: BBa_M36999
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_M36999/annotation2119821/range2119821/1

https://synbiohub.org/public/igem/BBa_M36999/annotation2119821/range2119821/1
type: Range
persistent identity: range2119821
display id: range2119821
version: 1
direction: direction/forward
top level: BBa_M36999
start: 79
end: 111
orientation: inline

BBa_K729016
type: ComponentDefinition
persistent identity: BBa K729016
display id: BBa_K729016
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Antifreeze protein, type I from Oceanibulbus indolifex HEL-45,
creator: Yeping Lu
created: 2012-09-20T11:00:00Z
modified: 2016-01-28T12:19:18Z
discontinued: false
dominant: false
experience: experience/None
group u list: _975_
m user id: 4206
owner id: 11514
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Antifreeze protein from Oceanibulbus indolifex
mutable provenance: Genomic DNA, http://www.ebi.ac.uk/ena/data/view/EDQ05862
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K729016_sequence/1

BBa_K1809025
type: ComponentDefinition
persistent identity: BBa K1809025
display id: BBa_K1809025
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TmAFP
creator: David Morgan
created: 2015-08-25T11:00:00Z
modified: 2016-01-27T10:36:08Z
discontinued: false
dominant: false
group u list: _2234_
m user id: 4206
owner id: 24831
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Antifreeze proteins (AFPs) are molecules with the unique property of binding and shaping ice crystals, preventing their growth into relatively large cell-lysing structures (Davies and Sykes 1997). They exhibit a property known as thermal hysteresis, whereby their activity decreases the freezing point of water without changing the melting point. This property allows organisms which produce AFPs to survive colder conditions. Additionally, AFPs have shown biolfilm inhibiting properties, potentially giving them uses in medical applications.
mutable notes: Codon optimized the sequence for E. coli and to minimize repetitive sequences; removed illegal restriction sites.
mutable provenance: Taken from the mealworm beetle Tenebrio molitor. Pubmed ID: 17443386
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1809025_sequence/1

BBa_K1809006
type: ComponentDefinition
persistent identity: BBa K1809006
display id: BBa_K1809006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: MaAFP
creator: Edward Dring
created: 2015-08-02T11:00:00Z
modified: 2016-01-27T10:33:14Z
discontinued: false
dominant: false
group u list: _2234_
m user id: 4206
owner id: 26152
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Antifreeze proteins (AFPs) are molecules with the unique property of binding and shaping ice crystals, preventing their growth into relatively large cell-lysing structures (Davies and Sykes 1997). They exhibit a property known as thermal hysteresis, whereby their activity decreases the freezing point of water without changing the melting point. This property allows organisms which produce AFPs to survive colder conditions. Additionally, AFPs have shown biolfilm inhibbiting properties, potetnially giving them uses in medical applications.
mutable notes: Codon optimized the sequence for E. coli and to minimize repetitive sequences; removed illegal restriction sites.
mutable provenance: Taken from the ocean pout Macrozarces americanus. Pubmed ID: 3840475
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: MaAFP
sequence: https://synbiohub.org/public/igem/BBa_K1809006_sequence/1

MaAFP
type: SequenceAnnotation
persistent identity: annotation2433990
display id: annotation2433990
version: 1
top level: BBa_K1809006
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1809006/annotation2433990/range2433990/1

https://synbiohub.org/public/igem/BBa_K1809006/annotation2433990/range2433990/1
type: Range
persistent identity: range2433990
display id: range2433990
version: 1
direction: direction/forward
top level: BBa_K1809006
start: 1
end: 264
orientation: inline

BBa_M36996
type: ComponentDefinition
persistent identity: BBa M36996
display id: BBa_M36996
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Non-Glycolated Antifreeze Protein (AFP) - 10-Repeated Segment
creator: Nathan Barnett, Meghan Bowler, Kian Torabian
created: 2011-04-27T11:00:00Z
modified: 2016-01-27T10:45:52Z
discontinued: false
dominant: false
experience: experience/None
group u list: _848_
m user id: 4206
owner id: 9543
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: The standard non-glycolated antifreeze protein (AFP) from the Pseudopleuronecta americanus. Begins with a chain of C's and is followed by 10 rough repeats of "ANAAAAAALTA". The codons have been optimized for E. coli.
mutable notes: This sequence has 10 rough repeats of "ANAAAAAALTA", as compared to the natural peptide which has this sequence repeated roughly 5 times.
mutable provenance: Based on the 5-repeated Segment AFP from the white flounder, Pseudopleuronecta americanus.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Unit 8 (TAANAAAAAAL)
sequence annotation: start
sequence annotation: C - linker
sequence annotation: Unit 1 (TAANAAAAAAL)
sequence annotation: Unit 9 (TAANAAAAAAL)
sequence annotation: Unit 3 (TAANAAAAAAL)
sequence annotation: Unit 4 (TAANAAAAAAL)
sequence annotation: Unit 2 (TAANAAAAAAL)
sequence annotation: Unit 7 (TAANAAAAAAL)
sequence annotation: Unit 5 (TAANAAAAAAL)
sequence annotation: stop
sequence annotation: Modified Unit 0 (TASDAAAAAL)
sequence annotation: Unit 6 (TAANAAAAAAL)
sequence: https://synbiohub.org/public/igem/BBa_M36996_sequence/1

Unit 8 (TAANAAAAAAL)
type: SequenceAnnotation
persistent identity: annotation2119809
display id: annotation2119809
version: 1
top level: BBa_M36996
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_M36996/annotation2119809/range2119809/1

https://synbiohub.org/public/igem/BBa_M36996/annotation2119809/range2119809/1
type: Range
persistent identity: range2119809
display id: range2119809
version: 1
direction: direction/forward
top level: BBa_M36996
start: 277
end: 309
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2119800
display id: annotation2119800
version: 1
top level: BBa_M36996
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_M36996/annotation2119800/range2119800/1

https://synbiohub.org/public/igem/BBa_M36996/annotation2119800/range2119800/1
type: Range
persistent identity: range2119800
display id: range2119800
version: 1
direction: direction/forward
top level: BBa_M36996
start: 10
end: 12
orientation: inline

C - linker
type: SequenceAnnotation
persistent identity: annotation2119799
display id: annotation2119799
version: 1
top level: BBa_M36996
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_M36996/annotation2119799/range2119799/1

https://synbiohub.org/public/igem/BBa_M36996/annotation2119799/range2119799/1
type: Range
persistent identity: range2119799
display id: range2119799
version: 1
direction: direction/forward
top level: BBa_M36996
start: 1
end: 9
orientation: inline

Unit 1 (TAANAAAAAAL)
type: SequenceAnnotation
persistent identity: annotation2119802
display id: annotation2119802
version: 1
top level: BBa_M36996
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_M36996/annotation2119802/range2119802/1

https://synbiohub.org/public/igem/BBa_M36996/annotation2119802/range2119802/1
type: Range
persistent identity: range2119802
display id: range2119802
version: 1
direction: direction/forward
top level: BBa_M36996
start: 46
end: 78
orientation: inline

Unit 9 (TAANAAAAAAL)
type: SequenceAnnotation
persistent identity: annotation2119810
display id: annotation2119810
version: 1
top level: BBa_M36996
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_M36996/annotation2119810/range2119810/1

https://synbiohub.org/public/igem/BBa_M36996/annotation2119810/range2119810/1
type: Range
persistent identity: range2119810
display id: range2119810
version: 1
direction: direction/forward
top level: BBa_M36996
start: 310
end: 342
orientation: inline

Unit 3 (TAANAAAAAAL)
type: SequenceAnnotation
persistent identity: annotation2119804
display id: annotation2119804
version: 1
top level: BBa_M36996
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_M36996/annotation2119804/range2119804/1

https://synbiohub.org/public/igem/BBa_M36996/annotation2119804/range2119804/1
type: Range
persistent identity: range2119804
display id: range2119804
version: 1
direction: direction/forward
top level: BBa_M36996
start: 112
end: 144
orientation: inline

Unit 4 (TAANAAAAAAL)
type: SequenceAnnotation
persistent identity: annotation2119805
display id: annotation2119805
version: 1
top level: BBa_M36996
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_M36996/annotation2119805/range2119805/1

https://synbiohub.org/public/igem/BBa_M36996/annotation2119805/range2119805/1
type: Range
persistent identity: range2119805
display id: range2119805
version: 1
direction: direction/forward
top level: BBa_M36996
start: 145
end: 177
orientation: inline

Unit 2 (TAANAAAAAAL)
type: SequenceAnnotation
persistent identity: annotation2119803
display id: annotation2119803
version: 1
top level: BBa_M36996
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_M36996/annotation2119803/range2119803/1

https://synbiohub.org/public/igem/BBa_M36996/annotation2119803/range2119803/1
type: Range
persistent identity: range2119803
display id: range2119803
version: 1
direction: direction/forward
top level: BBa_M36996
start: 79
end: 111
orientation: inline

Unit 7 (TAANAAAAAAL)
type: SequenceAnnotation
persistent identity: annotation2119808
display id: annotation2119808
version: 1
top level: BBa_M36996
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_M36996/annotation2119808/range2119808/1

https://synbiohub.org/public/igem/BBa_M36996/annotation2119808/range2119808/1
type: Range
persistent identity: range2119808
display id: range2119808
version: 1
direction: direction/forward
top level: BBa_M36996
start: 244
end: 276
orientation: inline

Unit 5 (TAANAAAAAAL)
type: SequenceAnnotation
persistent identity: annotation2119806
display id: annotation2119806
version: 1
top level: BBa_M36996
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_M36996/annotation2119806/range2119806/1

https://synbiohub.org/public/igem/BBa_M36996/annotation2119806/range2119806/1
type: Range
persistent identity: range2119806
display id: range2119806
version: 1
direction: direction/forward
top level: BBa_M36996
start: 178
end: 210
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2119811
display id: annotation2119811
version: 1
top level: BBa_M36996
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_M36996/annotation2119811/range2119811/1

https://synbiohub.org/public/igem/BBa_M36996/annotation2119811/range2119811/1
type: Range
persistent identity: range2119811
display id: range2119811
version: 1
direction: direction/forward
top level: BBa_M36996
start: 355
end: 360
orientation: inline

Modified Unit 0 (TASDAAAAAL)
type: SequenceAnnotation
persistent identity: annotation2119801
display id: annotation2119801
version: 1
top level: BBa_M36996
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_M36996/annotation2119801/range2119801/1

https://synbiohub.org/public/igem/BBa_M36996/annotation2119801/range2119801/1
type: Range
persistent identity: range2119801
display id: range2119801
version: 1
direction: direction/forward
top level: BBa_M36996
start: 16
end: 45
orientation: inline

Unit 6 (TAANAAAAAAL)
type: SequenceAnnotation
persistent identity: annotation2119807
display id: annotation2119807
version: 1
top level: BBa_M36996
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_M36996/annotation2119807/range2119807/1

https://synbiohub.org/public/igem/BBa_M36996/annotation2119807/range2119807/1
type: Range
persistent identity: range2119807
display id: range2119807
version: 1
direction: direction/forward
top level: BBa_M36996
start: 211
end: 243
orientation: inline

BBa_K1809015
type: ComponentDefinition
persistent identity: BBa K1809015
display id: BBa_K1809015
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: ChAFP
creator: Edward Dring
created: 2015-08-02T11:00:00Z
modified: 2016-01-27T10:34:59Z
discontinued: false
dominant: false
group u list: _2234_
m user id: 4206
owner id: 26152
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Antifreeze proteins (AFPs) are molecules with the unique property of binding and shaping ice crystals, preventing their growth into relatively large cell-lysing structures (Davies and Sykes 1997). They exhibit a property known as thermal hysteresis, whereby their activity decreases the freezing point of water without changing the melting point. This property allows organisms which produce AFPs to survive colder conditions. Additionally, AFPs have shown biolfilm inhibiting properties, potentially giving them uses in medical applications.
mutable notes: Codon optimized the sequence for E. coli and to minimize repetitive sequences; removed illegal restriction sites.
mutable provenance: Taken from the Atlantic herring Clupea harengus. Pubmed ID: 9521729
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: ChAFP
sequence: https://synbiohub.org/public/igem/BBa_K1809015_sequence/1

ChAFP
type: SequenceAnnotation
persistent identity: annotation2434157
display id: annotation2434157
version: 1
top level: BBa_K1809015
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1809015/annotation2434157/range2434157/1

https://synbiohub.org/public/igem/BBa_K1809015/annotation2434157/range2434157/1
type: Range
persistent identity: range2434157
display id: range2434157
version: 1
direction: direction/forward
top level: BBa_K1809015
start: 1
end: 447
orientation: inline

BBa_K1809018
type: ComponentDefinition
persistent identity: BBa K1809018
display id: BBa_K1809018
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: DcAFP
creator: Edward Dring
created: 2015-08-02T11:00:00Z
modified: 2016-02-12T09:28:48Z
discontinued: false
dominant: false
group u list: _2234_
m user id: 4206
owner id: 26152
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Antifreeze proteins (AFPs) are molecules with the unique property of binding and shaping ice crystals, preventing their growth into relatively large cell-lysing structures (Davies and Sykes 1997). They exhibit a property known as thermal hysteresis, whereby their activity decreases the freezing point of water without changing the melting point. This property allows organisms which produce AFPs to survive colder conditions. Additionally, AFPs have shown biolfilm inhibiting properties, potentially giving them uses in medical applications.
mutable notes: Codon optimized the sequence for E. coli and to minimize repetitive sequences; removed illegal restriction sites.
mutable provenance: Taken from carrot (Daucus carota). Pubmed ID: 9756474
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: DcAFP
sequence: https://synbiohub.org/public/igem/BBa_K1809018_sequence/1

DcAFP
type: SequenceAnnotation
persistent identity: annotation2434158
display id: annotation2434158
version: 1
top level: BBa_K1809018
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1809018/annotation2434158/range2434158/1

https://synbiohub.org/public/igem/BBa_K1809018/annotation2434158/range2434158/1
type: Range
persistent identity: range2434158
display id: range2434158
version: 1
direction: direction/forward
top level: BBa_K1809018
start: 1
end: 999
orientation: inline

BBa_K1809003
type: ComponentDefinition
persistent identity: BBa K1809003
display id: BBa_K1809003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RiAFP
creator: Edward Dring
created: 2015-08-02T11:00:00Z
modified: 2016-01-27T10:33:32Z
discontinued: false
dominant: false
group u list: _2234_
m user id: 4206
owner id: 26152
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Antifreeze proteins (AFPs) are molecules with the unique property of binding and shaping ice crystals, preventing their growth into relatively large cell-lysing structures (Davies and Sykes 1997). They exhibit a property known as thermal hysteresis, whereby their activity decreases the freezing point of water without changing the melting point. This property allows organisms which produce AFPs to survive colder conditions. Additionally, AFPs have shown biolfilm inhibbiting properties, potetnially giving them uses in medical applications.
mutable notes: Codon optimized the sequence for E. coli and to minimize repetitive sequences; removed illegal restriction sites.
mutable provenance: Taken from the longhorn beetle Rhagium inquisitor. Pubmed ID: 21078390
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: RiAFP
sequence: https://synbiohub.org/public/igem/BBa_K1809003_sequence/1

RiAFP
type: SequenceAnnotation
persistent identity: annotation2433989
display id: annotation2433989
version: 1
top level: BBa_K1809003
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1809003/annotation2433989/range2433989/1

https://synbiohub.org/public/igem/BBa_K1809003/annotation2433989/range2433989/1
type: Range
persistent identity: range2433989
display id: range2433989
version: 1
direction: direction/forward
top level: BBa_K1809003
start: 1
end: 336
orientation: inline

BBa_K1831003
type: ComponentDefinition
persistent identity: BBa K1831003
display id: BBa_K1831003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Type III AFP with E-coil
creator: Justine Ring, Morgan Litschko, Malak Elbatarny, Joanna Semrau,
created: 2015-09-10T11:00:00Z
modified: 2016-01-27T10:39:40Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2257_
m user id: 4206
owner id: 27228
owning group id: 9
status: status/Planning
bookmark: false
mutable description: What: This BioBrick is a fusion protein consisting of the E-coil domain of our coiled-coil system fused in-frame to the C-terminus of a Type III AFP. Type III AFP (PDB fIle: 1AME) is a moderately active antifreeze protein from the ocean pout, with a small (7Kb), globular structure. The E-coil refers to the coiled-coil with glutamic acid residues in the ???e??? and ???g??? positions of the helical wheel first used by Team Calgary 2013 (BBa_K1189011). This protein has been shown to be purified effectively by ice-affinity. If a His-tag is required for your purposes, see (BBa_K1831001). Why: The fusion of the E-coil to the Type III AFP will enable non-covalent, yet high affinity, specific attachment of AFPs to our protein scaffold and ???K??? coil BioBrick (BBa_K1831002). The interaction between the ???E??? and ???K??? coils involved oppositely charged residues, promoting the heterodimeric interaction of the coils that links AFPs to our scaffold. There is no His-tag, but ice-affinity purification can be used. By including a His-tag on our K-coil fusion protein (our scaffold: BBa_K1831002), and not the AFP with E-coil, we have enabled simple separation of any non-interacting coiled-coil domains.
mutable notes: The TypeIII AFP was fused in-frame to the E-coil through a gly-ser linker. The gly-ser coding DNA used the specific codons for a BamHI site, which is a unique cut site not found in either the AFP, E-coil, or the pSB1C3 backbone. Thus if you require the part in a different backbone, simple confirmation on cloning by a single restriction digest is possible.
mutable provenance: The TypeIII AFP (PDB: 1AME) was obtained from Dr. Peter Davies lab at Queen's University. The E-coil was a novel sequence based off the E-coil (BBa_K1189012) and K-coil (BBa_K1189013) pair designed by Team Calgary 2013
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: BamHI site
sequence annotation: Type III AFP
sequence annotation: E-coil
sequence annotation: stop x2
sequence annotation: T7 rbs
sequence annotation: NdeI site
sequence annotation: XhoI site
sequence: https://synbiohub.org/public/igem/BBa_K1831003_sequence/1

BamHI site
type: SequenceAnnotation
persistent identity: annotation2449992
display id: annotation2449992
version: 1
top level: BBa_K1831003
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1831003/annotation2449992/range2449992/1

https://synbiohub.org/public/igem/BBa_K1831003/annotation2449992/range2449992/1
type: Range
persistent identity: range2449992
display id: range2449992
version: 1
direction: direction/forward
top level: BBa_K1831003
start: 331
end: 336
orientation: inline

Type III AFP
type: SequenceAnnotation
persistent identity: annotation2449990
display id: annotation2449990
version: 1
top level: BBa_K1831003
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1831003/annotation2449990/range2449990/1

https://synbiohub.org/public/igem/BBa_K1831003/annotation2449990/range2449990/1
type: Range
persistent identity: range2449990
display id: range2449990
version: 1
direction: direction/forward
top level: BBa_K1831003
start: 68
end: 267
orientation: inline

E-coil
type: SequenceAnnotation
persistent identity: annotation2449991
display id: annotation2449991
version: 1
top level: BBa_K1831003
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1831003/annotation2449991/range2449991/1

https://synbiohub.org/public/igem/BBa_K1831003/annotation2449991/range2449991/1
type: Range
persistent identity: range2449991
display id: range2449991
version: 1
direction: direction/forward
top level: BBa_K1831003
start: 268
end: 330
orientation: inline

stop x2
type: SequenceAnnotation
persistent identity: annotation2450054
display id: annotation2450054
version: 1
top level: BBa_K1831003
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1831003/annotation2450054/range2450054/1

https://synbiohub.org/public/igem/BBa_K1831003/annotation2450054/range2450054/1
type: Range
persistent identity: range2450054
display id: range2450054
version: 1
direction: direction/forward
top level: BBa_K1831003
start: 337
end: 342
orientation: inline

T7 rbs
type: SequenceAnnotation
persistent identity: annotation2449988
display id: annotation2449988
version: 1
top level: BBa_K1831003
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1831003/annotation2449988/range2449988/1

https://synbiohub.org/public/igem/BBa_K1831003/annotation2449988/range2449988/1
type: Range
persistent identity: range2449988
display id: range2449988
version: 1
direction: direction/forward
top level: BBa_K1831003
start: 1
end: 61
orientation: inline

NdeI site
type: SequenceAnnotation
persistent identity: annotation2449989
display id: annotation2449989
version: 1
top level: BBa_K1831003
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1831003/annotation2449989/range2449989/1

https://synbiohub.org/public/igem/BBa_K1831003/annotation2449989/range2449989/1
type: Range
persistent identity: range2449989
display id: range2449989
version: 1
direction: direction/forward
top level: BBa_K1831003
start: 62
end: 67
orientation: inline

XhoI site
type: SequenceAnnotation
persistent identity: annotation2450055
display id: annotation2450055
version: 1
top level: BBa_K1831003
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1831003/annotation2450055/range2450055/1

https://synbiohub.org/public/igem/BBa_K1831003/annotation2450055/range2450055/1
type: Range
persistent identity: range2450055
display id: range2450055
version: 1
direction: direction/forward
top level: BBa_K1831003
start: 343
end: 348
orientation: inline

AFP
type: ComponentDefinition
persistent identity: BBa M36850
display id: BBa_M36850
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: HeLa Optimized Type I Antifreeze Protein
creator: Karen Gomez, Ivy Nguyen, Yoo Hsiu Yeh
created: 2012-12-06T12:00:00Z
modified: 2016-01-28T12:19:58Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _848_
m user id: 4206
owner id: 15026
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: We are designing a HeLa cell actuator that produces Type I antifreeze protein. The antifreeze protein we chose is naturally found in winter flounder serum and is a Type I AFP, known as AFP9. This amino acid sequence was published in a paper by Chao in 1996. It is very short, consisting of only 52 amino acids, in which there are three repeats of the same 9-amino acid section. The amino acid content is very Alanine-rich. Our designed part is a patchwork of components all chosen to maximize the output of our antifreeze protein. The CMV promoter, a constitutive gene that is expressed at a constant level, was used in order to encourage the maximum transcription of our target gene in the HeLa cells. The antifreeze protein sequence starts with a spacer, made up primarily of G???s and C???s to increase the guanine and cytosine content in our sequence and provide room for the ribosome to bind. This is followed by DNA2.0 Gene Designer software???s template kozak sequence, which is strong and promotes ribosome binding well. The kozak is then followed by the start codon, ATG, and the optimized antifreeze protein sequence. The protein sequence is followed by the stop codon and a terminator sequence, SV40 poly A, preset in the Gene Designer template. SV40 is used for "high level expression". The antibiotic resistance marker is the default ampicillin. The AFP has not been shown to be toxic to cells, so in order to produce as much of the protein as possible, the origin of replication strength is high.
mutable notes: We used the OPTIMIZER software with standard human codons at a guided random mix to generate the DNA sequence for the amino acid, then further manually modified some of the codons to eliminate repeated sequences.
mutable provenance: The amino acid sequence for this protein was published in the paper "A natural variant of type I antifreeze protein with four ice-binding repeats is a particularly potent antifreeze", by Heman Chao, et al. in Protein Science, Vol 5, Issue 6, pages 1150???1156 in June 1996. We optimized the nucleotides for expression in human cells.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: TAG
sequence annotation: Kozak
sequence annotation: ATG
sequence annotation: Antifreeze Protein
sequence: https://synbiohub.org/public/igem/BBa_M36850_sequence/1

TAG
type: SequenceAnnotation
persistent identity: annotation2213642
display id: annotation2213642
version: 1
top level: AFP
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_M36850/annotation2213642/range2213642/1

https://synbiohub.org/public/igem/BBa_M36850/annotation2213642/range2213642/1
type: Range
persistent identity: range2213642
display id: range2213642
version: 1
direction: direction/forward
top level: AFP
start: 166
end: 168
orientation: inline

Kozak
type: SequenceAnnotation
persistent identity: annotation2213643
display id: annotation2213643
version: 1
top level: AFP
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_M36850/annotation2213643/range2213643/1

https://synbiohub.org/public/igem/BBa_M36850/annotation2213643/range2213643/1
type: Range
persistent identity: range2213643
display id: range2213643
version: 1
direction: direction/forward
top level: AFP
start: 1
end: 3
orientation: inline

ATG
type: SequenceAnnotation
persistent identity: annotation2213641
display id: annotation2213641
version: 1
top level: AFP
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_M36850/annotation2213641/range2213641/1

https://synbiohub.org/public/igem/BBa_M36850/annotation2213641/range2213641/1
type: Range
persistent identity: range2213641
display id: range2213641
version: 1
direction: direction/forward
top level: AFP
start: 4
end: 6
orientation: inline

Antifreeze Protein
type: SequenceAnnotation
persistent identity: annotation2213644
display id: annotation2213644
version: 1
top level: AFP
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_M36850/annotation2213644/range2213644/1

https://synbiohub.org/public/igem/BBa_M36850/annotation2213644/range2213644/1
type: Range
persistent identity: range2213644
display id: range2213644
version: 1
direction: direction/forward
top level: AFP
start: 7
end: 165
orientation: inline

BBa_K1809012
type: ComponentDefinition
persistent identity: BBa K1809012
display id: BBa_K1809012
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TiAFP
creator: Edward Dring
created: 2015-08-02T11:00:00Z
modified: 2016-01-27T10:34:40Z
discontinued: false
dominant: false
group u list: _2234_
m user id: 4206
owner id: 26152
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Antifreeze proteins (AFPs) are molecules with the unique property of binding and shaping ice crystals, preventing their growth into relatively large cell-lysing structures (Davies and Sykes 1997). They exhibit a property known as thermal hysteresis, whereby their activity decreases the freezing point of water without changing the melting point. This property allows organisms which produce AFPs to survive colder conditions. Additionally, AFPs have shown biolfilm inhibbiting properties, potetnially giving them uses in medical applications.
mutable notes: Codon optimized the sequence for E. coli and to minimize repetitive sequences; removed illegal restriction sites.
mutable provenance: Taken from the fungus Typhula ishikariensis. Pubmed ID: 20030710
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: TiAFP
sequence: https://synbiohub.org/public/igem/BBa_K1809012_sequence/1

TiAFP
type: SequenceAnnotation
persistent identity: annotation2433992
display id: annotation2433992
version: 1
top level: BBa_K1809012
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1809012/annotation2433992/range2433992/1

https://synbiohub.org/public/igem/BBa_K1809012/annotation2433992/range2433992/1
type: Range
persistent identity: range2433992
display id: range2433992
version: 1
direction: direction/forward
top level: BBa_K1809012
start: 1
end: 732
orientation: inline

BBa_M36951
type: ComponentDefinition
persistent identity: BBa M36951
display id: BBa_M36951
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Insect antifreeze protein (RiAFP) Chain B
creator: Amiel Stephen Paz
created: 2015-10-23T11:00:00Z
modified: 2016-01-27T10:37:26Z
discontinued: false
dominant: false
group u list: _848_
m user id: 4206
owner id: 29120
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Codes for Chain B of the Rhagium inquisitor antifreeze protein - sequence optimized for human cell lines.
mutable notes: Codon optimization for human cell lines.
mutable provenance: Rhagium inquisitor genome.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_M36951_sequence/1

BBa_K1809036
type: ComponentDefinition
persistent identity: BBa K1809036
display id: BBa_K1809036
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Const. Promoter-RBS-BclA-IAFGP-DT
creator: David Morgan
created: 2015-09-02T11:00:00Z
modified: 2016-02-12T09:31:15Z
discontinued: false
dominant: false
group u list: _2234_
m user id: 4206
owner id: 24831
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This part contains the constitutive promoter BBa_J23100, an RBS, the cell surface expression tag BclA, Ixodes capularis antifreeze glycoprotein (IAFGP), and a double terminator. It encodes an antifreeze protein that attaches to the cell surface.
mutable notes: Spliced together a cell surface expression tag and the antifreeze protein to test cell surface activity.
mutable provenance: Composite of parts BBa_K1423005 and BBa_K1809035 without the YFP tag.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1809035
component: BBa_B0034
component: BBa_K1423000
component: BBa_B0010
component: BBa_B0012
component: BBa_J23100
sequence annotation: BBa_B0012
sequence annotation: BBa_K1809035
sequence annotation: BBa_K1423000
sequence annotation: BBa_B0010
sequence annotation: BBa_J23100
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K1809036_sequence/1

BBa_K1809035
type: Component
persistent identity: component2443204
display id: component2443204
version: 1
top level: BBa_K1809036
access: public
definition: https://synbiohub.org/public/igem/BBa_K1809035/1

BBa_B0034
type: Component
persistent identity: component2443202
display id: component2443202
version: 1
top level: BBa_K1809036
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K1423000
type: Component
persistent identity: component2443203
display id: component2443203
version: 1
top level: BBa_K1809036
access: public
definition: https://synbiohub.org/public/igem/BBa_K1423000/1

BBa_B0010
type: Component
persistent identity: component2443205
display id: component2443205
version: 1
top level: BBa_K1809036
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
type: Component
persistent identity: component2443207
display id: component2443207
version: 1
top level: BBa_K1809036
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_J23100
type: Component
persistent identity: component2443200
display id: component2443200
version: 1
top level: BBa_K1809036
access: public
definition: https://synbiohub.org/public/igem/BBa_J23100/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2443207
display id: annotation2443207
version: 1
top level: BBa_K1809036
location: https://synbiohub.org/public/igem/BBa_K1809036/annotation2443207/range2443207/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K1809036/annotation2443207/range2443207/1
type: Range
persistent identity: range2443207
display id: range2443207
version: 1
direction: direction/forward
top level: BBa_K1809036
start: 499
end: 539
orientation: inline

BBa_K1809035
type: SequenceAnnotation
persistent identity: annotation2443204
display id: annotation2443204
version: 1
top level: BBa_K1809036
location: https://synbiohub.org/public/igem/BBa_K1809036/annotation2443204/range2443204/1
component: BBa_K1809035

https://synbiohub.org/public/igem/BBa_K1809036/annotation2443204/range2443204/1
type: Range
persistent identity: range2443204
display id: range2443204
version: 1
direction: direction/reverse
top level: BBa_K1809036
start: 133
end: 402
orientation: inline

BBa_K1423000
type: SequenceAnnotation
persistent identity: annotation2443203
display id: annotation2443203
version: 1
top level: BBa_K1809036
location: https://synbiohub.org/public/igem/BBa_K1809036/annotation2443203/range2443203/1
component: BBa_K1423000

https://synbiohub.org/public/igem/BBa_K1809036/annotation2443203/range2443203/1
type: Range
persistent identity: range2443203
display id: range2443203
version: 1
direction: direction/reverse
top level: BBa_K1809036
start: 64
end: 126
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2443205
display id: annotation2443205
version: 1
top level: BBa_K1809036
location: https://synbiohub.org/public/igem/BBa_K1809036/annotation2443205/range2443205/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K1809036/annotation2443205/range2443205/1
type: Range
persistent identity: range2443205
display id: range2443205
version: 1
direction: direction/forward
top level: BBa_K1809036
start: 411
end: 490
orientation: inline

BBa_J23100
type: SequenceAnnotation
persistent identity: annotation2443200
display id: annotation2443200
version: 1
top level: BBa_K1809036
location: https://synbiohub.org/public/igem/BBa_K1809036/annotation2443200/range2443200/1
component: BBa_J23100

https://synbiohub.org/public/igem/BBa_K1809036/annotation2443200/range2443200/1
type: Range
persistent identity: range2443200
display id: range2443200
version: 1
direction: direction/reverse
top level: BBa_K1809036
start: 1
end: 35
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2443202
display id: annotation2443202
version: 1
top level: BBa_K1809036
location: https://synbiohub.org/public/igem/BBa_K1809036/annotation2443202/range2443202/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1809036/annotation2443202/range2443202/1
type: Range
persistent identity: range2443202
display id: range2443202
version: 1
direction: direction/reverse
top level: BBa_K1809036
start: 44
end: 55
orientation: inline

BBa_K729018
type: ComponentDefinition
persistent identity: BBa K729018
display id: BBa_K729018
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: J23119 - RBS - csgBAEFG
creator: Bouran Sohrabi
created: 2012-09-21T11:00:00Z
modified: 2016-02-12T09:31:57Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _975_
m user id: 4206
owner id: 11516
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: -
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K729003
component: BBa_J23119
component: BBa_B0034
sequence annotation: BBa_B0034
sequence annotation: BBa_J23119
sequence annotation: BBa_K729003
sequence: https://synbiohub.org/public/igem/BBa_K729018_sequence/1

BBa_K729003
type: Component
persistent identity: component2205464
display id: component2205464
version: 1
top level: BBa_K729018
access: public
definition: https://synbiohub.org/public/igem/BBa_K729003/1

BBa_J23119
type: Component
persistent identity: component2205460
display id: component2205460
version: 1
top level: BBa_K729018
access: public
definition: https://synbiohub.org/public/igem/BBa_J23119/1

BBa_B0034
type: Component
persistent identity: component2205462
display id: component2205462
version: 1
top level: BBa_K729018
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2205462
display id: annotation2205462
version: 1
top level: BBa_K729018
location: https://synbiohub.org/public/igem/BBa_K729018/annotation2205462/range2205462/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K729018/annotation2205462/range2205462/1
type: Range
persistent identity: range2205462
display id: range2205462
version: 1
direction: direction/reverse
top level: BBa_K729018
start: 44
end: 55
orientation: inline

BBa_J23119
type: SequenceAnnotation
persistent identity: annotation2205460
display id: annotation2205460
version: 1
top level: BBa_K729018
location: https://synbiohub.org/public/igem/BBa_K729018/annotation2205460/range2205460/1
component: BBa_J23119

https://synbiohub.org/public/igem/BBa_K729018/annotation2205460/range2205460/1
type: Range
persistent identity: range2205460
display id: range2205460
version: 1
direction: direction/reverse
top level: BBa_K729018
start: 1
end: 35
orientation: inline

BBa_K729003
type: SequenceAnnotation
persistent identity: annotation2205464
display id: annotation2205464
version: 1
top level: BBa_K729018
location: https://synbiohub.org/public/igem/BBa_K729018/annotation2205464/range2205464/1
component: BBa_K729003

https://synbiohub.org/public/igem/BBa_K729018/annotation2205464/range2205464/1
type: Range
persistent identity: range2205464
display id: range2205464
version: 1
direction: direction/reverse
top level: BBa_K729018
start: 64
end: 2761
orientation: inline

BBa_K1809000
type: ComponentDefinition
persistent identity: BBa K1809000
display id: BBa_K1809000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: GaAFP
creator: Edward Dring
created: 2015-07-21T11:00:00Z
modified: 2016-01-27T10:32:22Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2234_
m user id: 4206
owner id: 26152
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Antifreeze protein construct
mutable notes: Codon optimized the sequence for E. coli and to minimize repetitive sequences; removed illegal restriction sites.
mutable provenance: Composite of parts BBa_J23119, BBa_B0015, BBa_B0034 and BBa_K652004.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: GaAFP
sequence: https://synbiohub.org/public/igem/BBa_K1809000_sequence/1

GaAFP
type: SequenceAnnotation
persistent identity: annotation2433988
display id: annotation2433988
version: 1
top level: BBa_K1809000
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1809000/annotation2433988/range2433988/1

https://synbiohub.org/public/igem/BBa_K1809000/annotation2433988/range2433988/1
type: Range
persistent identity: range2433988
display id: range2433988
version: 1
direction: direction/forward
top level: BBa_K1809000
start: 1
end: 531
orientation: inline

BBa_K1809021
type: ComponentDefinition
persistent identity: BBa K1809021
display id: BBa_K1809021
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: ZeAFP
creator: David Morgan
created: 2015-08-25T11:00:00Z
modified: 2016-01-27T10:35:35Z
discontinued: false
dominant: false
group u list: _2234_
m user id: 4206
owner id: 24831
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Antifreeze proteins (AFPs) are molecules with the unique property of binding and shaping ice crystals, preventing their growth into relatively large cell-lysing structures (Davies and Sykes 1997). They exhibit a property known as thermal hysteresis, whereby their activity decreases the freezing point of water without changing the melting point. This property allows organisms which produce AFPs to survive colder conditions. Additionally, AFPs have shown biolfilm inhibiting properties, potentially giving them uses in medical applications.
mutable notes: Codon optimized the sequence for E. coli and to minimize repetitive sequences; removed illegal restriction sites.
mutable provenance: Isolated from the part BBa_K652004 taken from the 2015 distribution plate 1, well 10C.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1809021_sequence/1

BBa_K1809031
type: ComponentDefinition
persistent identity: BBa K1809031
display id: BBa_K1809031
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: MpAFP
creator: David Morgan
created: 2015-08-25T11:00:00Z
modified: 2016-01-27T10:37:00Z
discontinued: false
dominant: false
group u list: _2234_
m user id: 4206
owner id: 24831
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Antifreeze proteins (AFPs) are molecules with the unique property of binding and shaping ice crystals, preventing their growth into relatively large cell-lysing structures (Davies and Sykes 1997). They exhibit a property known as thermal hysteresis, whereby their activity decreases the freezing point of water without changing the melting point. This property allows organisms which produce AFPs to survive colder conditions. Additionally, AFPs have shown biolfilm inhibiting properties, potentially giving them uses in medical applications.
mutable notes: Codon optimized the sequence for E. coli and to minimize repetitive sequences; removed illegal restriction sites.
mutable provenance: Taken from the Antarctic bacterium Marinomonas primoryensis. Pubmed ID: 18095937
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1809031_sequence/1

BBa_K1809009
type: ComponentDefinition
persistent identity: BBa K1809009
display id: BBa_K1809009
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: EpAFP
creator: Edward Dring
created: 2015-08-02T11:00:00Z
modified: 2016-01-27T10:34:27Z
discontinued: false
dominant: false
group u list: _2234_
m user id: 4206
owner id: 26152
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Antifreeze proteins (AFPs) are molecules with the unique property of binding and shaping ice crystals, preventing their growth into relatively large cell-lysing structures (Davies and Sykes 1997). They exhibit a property known as thermal hysteresis, whereby their activity decreases the freezing point of water without changing the melting point. This property allows organisms which produce AFPs to survive colder conditions. Additionally, AFPs have shown biolfilm inhibbiting properties, potetnially giving them uses in medical applications.
mutable notes: Codon optimized the sequence for E. coli and to minimize repetitive sequences; removed illegal restriction sites.
mutable provenance: Taken from Escherichia phage 121Q. NCBI accession: YP_009102201
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: EpAFP
sequence: https://synbiohub.org/public/igem/BBa_K1809009_sequence/1

EpAFP
type: SequenceAnnotation
persistent identity: annotation2433991
display id: annotation2433991
version: 1
top level: BBa_K1809009
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1809009/annotation2433991/range2433991/1

https://synbiohub.org/public/igem/BBa_K1809009/annotation2433991/range2433991/1
type: Range
persistent identity: range2433991
display id: range2433991
version: 1
direction: direction/forward
top level: BBa_K1809009
start: 1
end: 204
orientation: inline

BBa_M36950
type: ComponentDefinition
persistent identity: BBa M36950
display id: BBa_M36950
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Insect antifreeze protein (RiAFP) Chain A
creator: Amiel Stephen Paz, William Kindschuh, Vickram Gidwani
created: 2015-10-23T11:00:00Z
modified: 2016-01-27T10:37:37Z
discontinued: false
dominant: false
experience: experience/None
group u list: _848_
m user id: 4206
owner id: 29120
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Codes for Chain A of the Rhagium inquisitor antifreeze protein - sequence optimized for human cell lines.
mutable notes: Codon optimization for human cell lines.
mutable provenance: Sequenced from the Rhagium inquisitor genome.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_M36950_sequence/1

BBa_K1809011
type: ComponentDefinition
persistent identity: BBa K1809011
display id: BBa_K1809011
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Const. Promoter-RBS-BclA-EpAFP-DT
creator: Edward Dring
created: 2015-08-02T11:00:00Z
modified: 2016-02-12T09:28:26Z
discontinued: false
dominant: false
group u list: _2234_
m user id: 4206
owner id: 26152
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This part contains the constitutive promoter BBa_J23100, an RBS, the cell surface expression tag BclA, EpAFP, and a double terminator. It encodes an antifreeze protein that attaches to the cell surface.
mutable notes: Spliced together a cell surface expression tag and the antifreeze protein to test cell surface activity.
mutable provenance: Composite of parts BBa_K1423005 and BBa_K1809028 without the YFP tag.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1809009
component: BBa_B0012
component: BBa_B0034
component: BBa_B0010
component: BBa_K1423000
component: BBa_J23100
sequence annotation: BBa_B0012
sequence annotation: BBa_B0010
sequence annotation: BBa_B0034
sequence annotation: BBa_J23100
sequence annotation: BBa_K1809009
sequence annotation: BBa_K1423000
sequence: https://synbiohub.org/public/igem/BBa_K1809011_sequence/1

BBa_K1809009
type: Component
persistent identity: component2434137
display id: component2434137
version: 1
top level: BBa_K1809011
access: public
definition: BBa_K1809009

BBa_B0012
type: Component
persistent identity: component2434140
display id: component2434140
version: 1
top level: BBa_K1809011
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0034
type: Component
persistent identity: component2434134
display id: component2434134
version: 1
top level: BBa_K1809011
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0010
type: Component
persistent identity: component2434138
display id: component2434138
version: 1
top level: BBa_K1809011
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_K1423000
type: Component
persistent identity: component2434135
display id: component2434135
version: 1
top level: BBa_K1809011
access: public
definition: https://synbiohub.org/public/igem/BBa_K1423000/1

BBa_J23100
type: Component
persistent identity: component2434132
display id: component2434132
version: 1
top level: BBa_K1809011
access: public
definition: https://synbiohub.org/public/igem/BBa_J23100/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2434140
display id: annotation2434140
version: 1
top level: BBa_K1809011
location: https://synbiohub.org/public/igem/BBa_K1809011/annotation2434140/range2434140/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K1809011/annotation2434140/range2434140/1
type: Range
persistent identity: range2434140
display id: range2434140
version: 1
direction: direction/forward
top level: BBa_K1809011
start: 433
end: 473
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2434138
display id: annotation2434138
version: 1
top level: BBa_K1809011
location: https://synbiohub.org/public/igem/BBa_K1809011/annotation2434138/range2434138/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K1809011/annotation2434138/range2434138/1
type: Range
persistent identity: range2434138
display id: range2434138
version: 1
direction: direction/forward
top level: BBa_K1809011
start: 345
end: 424
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2434134
display id: annotation2434134
version: 1
top level: BBa_K1809011
location: https://synbiohub.org/public/igem/BBa_K1809011/annotation2434134/range2434134/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1809011/annotation2434134/range2434134/1
type: Range
persistent identity: range2434134
display id: range2434134
version: 1
direction: direction/reverse
top level: BBa_K1809011
start: 44
end: 55
orientation: inline

BBa_J23100
type: SequenceAnnotation
persistent identity: annotation2434132
display id: annotation2434132
version: 1
top level: BBa_K1809011
location: https://synbiohub.org/public/igem/BBa_K1809011/annotation2434132/range2434132/1
component: BBa_J23100

https://synbiohub.org/public/igem/BBa_K1809011/annotation2434132/range2434132/1
type: Range
persistent identity: range2434132
display id: range2434132
version: 1
direction: direction/reverse
top level: BBa_K1809011
start: 1
end: 35
orientation: inline

BBa_K1809009
type: SequenceAnnotation
persistent identity: annotation2434137
display id: annotation2434137
version: 1
top level: BBa_K1809011
location: https://synbiohub.org/public/igem/BBa_K1809011/annotation2434137/range2434137/1
component: BBa_K1809009

https://synbiohub.org/public/igem/BBa_K1809011/annotation2434137/range2434137/1
type: Range
persistent identity: range2434137
display id: range2434137
version: 1
direction: direction/reverse
top level: BBa_K1809011
start: 133
end: 336
orientation: inline

BBa_K1423000
type: SequenceAnnotation
persistent identity: annotation2434135
display id: annotation2434135
version: 1
top level: BBa_K1809011
location: https://synbiohub.org/public/igem/BBa_K1809011/annotation2434135/range2434135/1
component: BBa_K1423000

https://synbiohub.org/public/igem/BBa_K1809011/annotation2434135/range2434135/1
type: Range
persistent identity: range2434135
display id: range2434135
version: 1
direction: direction/reverse
top level: BBa_K1809011
start: 64
end: 126
orientation: inline

BBa_K1809027
type: ComponentDefinition
persistent identity: BBa K1809027
display id: BBa_K1809027
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LpAFP
creator: David Morgan
created: 2015-08-25T11:00:00Z
modified: 2016-01-27T10:36:21Z
discontinued: false
dominant: false
group u list: _2234_
m user id: 4206
owner id: 24831
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Antifreeze proteins (AFPs) are molecules with the unique property of binding and shaping ice crystals, preventing their growth into relatively large cell-lysing structures (Davies and Sykes 1997). They exhibit a property known as thermal hysteresis, whereby their activity decreases the freezing point of water without changing the melting point. This property allows organisms which produce AFPs to survive colder conditions. Additionally, AFPs have shown biolfilm inhibiting properties, potentially giving them uses in medical applications.
mutable notes: Codon optimized the sequence for E. coli and to minimize repetitive sequences; removed illegal restriction sites.
mutable provenance: Taken from the rye grass Lolium perenne. Pubmed ID: 21457707
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1809027_sequence/1

UNIK Antif
type: ComponentDefinition
persistent identity: BBa K1825000
display id: BBa_K1825000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: UNIK antifreeze protein
creator: Adam Petersen
created: 2015-08-30T11:00:00Z
modified: 2016-01-27T10:30:03Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2251_
m user id: 4206
owner id: 28032
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This is the coding region of a small antifreeze protein. It works by binding to the ice crystals as they form inside the cell, and by doing so prevents the ice crystals from enlarging and penetrating the cell membrane. We wanted to test if there would be a significant difference in the growth rate of moss, Physcomitrella patens, with the antifreeze gene, compared to the one without. This would be done at a low temperature to test the in-vivo function of the antifreeze protein.
mutable notes: We used codon optimization for moss so it would work better in Physcomitrella Patens.
mutable provenance: The part is taken from a spruce budworm, Choristoneura fumiferana, living in areas with permafrost.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: startcodon
sequence: https://synbiohub.org/public/igem/BBa_K1825000_sequence/1

startcodon
type: SequenceAnnotation
persistent identity: annotation2440844
display id: annotation2440844
version: 1
top level: UNIK Antif
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1825000/annotation2440844/range2440844/1

https://synbiohub.org/public/igem/BBa_K1825000/annotation2440844/range2440844/1
type: Range
persistent identity: range2440844
display id: range2440844
version: 1
direction: direction/forward
top level: UNIK Antif
start: 1
end: 3
orientation: inline

BBa_K1831001
type: ComponentDefinition
persistent identity: BBa K1831001
display id: BBa_K1831001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TypeIII and AFP E-coil fusion + His tag
creator: Malak Elbatarny, Joanna Semrau, Justine Ring, Morgan Litschko
created: 2015-08-31T11:00:00Z
modified: 2016-01-27T10:38:57Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2257_
m user id: 4206
owner id: 27228
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This BioBrick is a fusion protein consisting of the ???E??? coil domain of our coiled-coil system fused in-frame to the C-terminus of a Type III AFP. Type III AFP (PDB fIle: 1AME) is a moderately active antifreeze protein from the ocean pout, with a small (7Kb), globular structure. The ???E??? coil refers to the coiled-coil with glutamic acid residues in the ???e??? and ???g??? positions of the helical wheel first used by Team Calgary 2013 (BBa_K1189011). There is a His-tag at the C-terminus of the ???E??? coil which provides an alternative purification method to ice-affinity. The fusion of the ???E??? coil to the Type III AFP will enable non-covalent, yet high affinity, specific attachment of AFPs to our protein scaffold and ???K??? coil BioBrick (BBa_K1831002). The interaction between the ???E??? and ???K??? coils involved oppositely charged residues, promoting the heterodimeric interaction of the coils that links AFPs to our scaffold. The addition of a His-tag to the C-terminus of the coil will allow for simple purification of the protein even for teams without access to an ice-affinity purification apparatus.
mutable notes: The TypeIII AFP was fused in-frame to the E-coil through a gly-ser linker. The gly-ser coding DNA used the specific codons for a BamHI site, which is a unique cut site not found in either the AFP, E-coil, or the pSB1C3 backbone. Thus if you require the part in a different backbone, simple confirmation on cloning by a single restriction digest is possible.
mutable provenance: The TypeIII AFP (PDB: 1AME) was obtained from Dr. Peter Davies lab at Queen's University. The E-coil was a novel sequence based off the E-coil (BBa_K1189012) and K-coil (BBa_K1189013) pair designed by Team Calgary 2013
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: His-tag
sequence annotation: XhoI site
sequence annotation: E-coil
sequence annotation: stop x2
sequence annotation: TypeIII AFP
sequence annotation: NdeI site
sequence annotation: BamHI site
sequence annotation: T7 rbs
sequence: https://synbiohub.org/public/igem/BBa_K1831001_sequence/1

His-tag
type: SequenceAnnotation
persistent identity: annotation2449966
display id: annotation2449966
version: 1
top level: BBa_K1831001
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1831001/annotation2449966/range2449966/1

https://synbiohub.org/public/igem/BBa_K1831001/annotation2449966/range2449966/1
type: Range
persistent identity: range2449966
display id: range2449966
version: 1
direction: direction/forward
top level: BBa_K1831001
start: 337
end: 351
orientation: inline

XhoI site
type: SequenceAnnotation
persistent identity: annotation2449967
display id: annotation2449967
version: 1
top level: BBa_K1831001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1831001/annotation2449967/range2449967/1

https://synbiohub.org/public/igem/BBa_K1831001/annotation2449967/range2449967/1
type: Range
persistent identity: range2449967
display id: range2449967
version: 1
direction: direction/forward
top level: BBa_K1831001
start: 358
end: 363
orientation: inline

E-coil
type: SequenceAnnotation
persistent identity: annotation2449964
display id: annotation2449964
version: 1
top level: BBa_K1831001
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1831001/annotation2449964/range2449964/1

https://synbiohub.org/public/igem/BBa_K1831001/annotation2449964/range2449964/1
type: Range
persistent identity: range2449964
display id: range2449964
version: 1
direction: direction/forward
top level: BBa_K1831001
start: 268
end: 330
orientation: inline

stop x2
type: SequenceAnnotation
persistent identity: annotation2450033
display id: annotation2450033
version: 1
top level: BBa_K1831001
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1831001/annotation2450033/range2450033/1

https://synbiohub.org/public/igem/BBa_K1831001/annotation2450033/range2450033/1
type: Range
persistent identity: range2450033
display id: range2450033
version: 1
direction: direction/forward
top level: BBa_K1831001
start: 352
end: 357
orientation: inline

TypeIII AFP
type: SequenceAnnotation
persistent identity: annotation2449963
display id: annotation2449963
version: 1
top level: BBa_K1831001
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1831001/annotation2449963/range2449963/1

https://synbiohub.org/public/igem/BBa_K1831001/annotation2449963/range2449963/1
type: Range
persistent identity: range2449963
display id: range2449963
version: 1
direction: direction/forward
top level: BBa_K1831001
start: 68
end: 267
orientation: inline

NdeI site
type: SequenceAnnotation
persistent identity: annotation2449962
display id: annotation2449962
version: 1
top level: BBa_K1831001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1831001/annotation2449962/range2449962/1

https://synbiohub.org/public/igem/BBa_K1831001/annotation2449962/range2449962/1
type: Range
persistent identity: range2449962
display id: range2449962
version: 1
direction: direction/forward
top level: BBa_K1831001
start: 62
end: 67
orientation: inline

BamHI site
type: SequenceAnnotation
persistent identity: annotation2449965
display id: annotation2449965
version: 1
top level: BBa_K1831001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1831001/annotation2449965/range2449965/1

https://synbiohub.org/public/igem/BBa_K1831001/annotation2449965/range2449965/1
type: Range
persistent identity: range2449965
display id: range2449965
version: 1
direction: direction/forward
top level: BBa_K1831001
start: 331
end: 336
orientation: inline

T7 rbs
type: SequenceAnnotation
persistent identity: annotation2449961
display id: annotation2449961
version: 1
top level: BBa_K1831001
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1831001/annotation2449961/range2449961/1

https://synbiohub.org/public/igem/BBa_K1831001/annotation2449961/range2449961/1
type: Range
persistent identity: range2449961
display id: range2449961
version: 1
direction: direction/forward
top level: BBa_K1831001
start: 1
end: 61
orientation: inline

BBa_M36997
type: ComponentDefinition
persistent identity: BBa M36997
display id: BBa_M36997
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Non-Glycolated Antifreeze Protein (AFP) - 1-Repeated Segment
creator: Nathan Barnett, Meghan Bowler, Kian Torabian
created: 2011-04-27T11:00:00Z
modified: 2016-01-27T10:45:22Z
discontinued: false
dominant: false
experience: experience/None
group u list: _848_
m user id: 4206
owner id: 9543
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: The standard non-glycolated antifreeze protein (AFP) from the Pseudopleuronecta americanus. The codons have been optimized for E. coli.
mutable notes: This is a reduced segment of the 5-Repeated Segment AFP, with four "ANAAAAAALTA" taken out.
mutable provenance: Comes from the white flounder, Pseudopleuronecta americanus.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Modified Unit 0 (TASDAAAAAL)
sequence annotation: c-linker
sequence annotation: start
sequence annotation: stop
sequence annotation: Unit 1 (TAANAAAAAAL)
sequence: https://synbiohub.org/public/igem/BBa_M36997_sequence/1

Modified Unit 0 (TASDAAAAAL)
type: SequenceAnnotation
persistent identity: annotation2119814
display id: annotation2119814
version: 1
top level: BBa_M36997
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_M36997/annotation2119814/range2119814/1

https://synbiohub.org/public/igem/BBa_M36997/annotation2119814/range2119814/1
type: Range
persistent identity: range2119814
display id: range2119814
version: 1
direction: direction/forward
top level: BBa_M36997
start: 16
end: 45
orientation: inline

c-linker
type: SequenceAnnotation
persistent identity: annotation2119812
display id: annotation2119812
version: 1
top level: BBa_M36997
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_M36997/annotation2119812/range2119812/1

https://synbiohub.org/public/igem/BBa_M36997/annotation2119812/range2119812/1
type: Range
persistent identity: range2119812
display id: range2119812
version: 1
direction: direction/forward
top level: BBa_M36997
start: 1
end: 9
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2119813
display id: annotation2119813
version: 1
top level: BBa_M36997
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_M36997/annotation2119813/range2119813/1

https://synbiohub.org/public/igem/BBa_M36997/annotation2119813/range2119813/1
type: Range
persistent identity: range2119813
display id: range2119813
version: 1
direction: direction/forward
top level: BBa_M36997
start: 10
end: 12
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2119816
display id: annotation2119816
version: 1
top level: BBa_M36997
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_M36997/annotation2119816/range2119816/1

https://synbiohub.org/public/igem/BBa_M36997/annotation2119816/range2119816/1
type: Range
persistent identity: range2119816
display id: range2119816
version: 1
direction: direction/forward
top level: BBa_M36997
start: 91
end: 96
orientation: inline

Unit 1 (TAANAAAAAAL)
type: SequenceAnnotation
persistent identity: annotation2119815
display id: annotation2119815
version: 1
top level: BBa_M36997
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_M36997/annotation2119815/range2119815/1

https://synbiohub.org/public/igem/BBa_M36997/annotation2119815/range2119815/1
type: Range
persistent identity: range2119815
display id: range2119815
version: 1
direction: direction/forward
top level: BBa_M36997
start: 46
end: 78
orientation: inline

BBa_K1809023
type: ComponentDefinition
persistent identity: BBa K1809023
display id: BBa_K1809023
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: BrAFP
creator: David Morgan
created: 2015-08-25T11:00:00Z
modified: 2016-01-27T10:35:50Z
discontinued: false
dominant: false
group u list: _2234_
m user id: 4206
owner id: 24831
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Antifreeze proteins (AFPs) are molecules with the unique property of binding and shaping ice crystals, preventing their growth into relatively large cell-lysing structures (Davies and Sykes 1997). They exhibit a property known as thermal hysteresis, whereby their activity decreases the freezing point of water without changing the melting point. This property allows organisms which produce AFPs to survive colder conditions. Additionally, AFPs have shown biolfilm inhibiting properties, potentially giving them uses in medical applications.
mutable notes: Codon optimized the sequence for E. coli and to minimize repetitive sequences; removed illegal restriction sites.
mutable provenance: Taken from the fish Brachyosis rostratus. Pubmed ID: 16754975
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1809023_sequence/1

BBa_K1809033
type: ComponentDefinition
persistent identity: BBa K1809033
display id: BBa_K1809033
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CfAFP
creator: David Morgan
created: 2015-08-25T11:00:00Z
modified: 2016-01-27T10:37:13Z
discontinued: false
dominant: false
group u list: _2234_
m user id: 4206
owner id: 24831
owning group id: 9
status: status/Planning
bookmark: false
mutable description: Antifreeze proteins (AFPs) are molecules with the unique property of binding and shaping ice crystals, preventing their growth into relatively large cell-lysing structures (Davies and Sykes 1997). They exhibit a property known as thermal hysteresis, whereby their activity decreases the freezing point of water without changing the melting point. This property allows organisms which produce AFPs to survive colder conditions. Additionally, AFPs have shown biolfilm inhibiting properties, potentially giving them uses in medical applications.
mutable notes: Codon optimized the sequence for E. coli and to minimize repetitive sequences; removed illegal restriction sites.
mutable provenance: Taken from the spruce budworm Choristoneura fumiferana. Pubmed ID: 9306405
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1809033_sequence/1