//classic/signalling/sender
type: Collection
persistent identity: classic signalling sender
display id: classic_signalling_sender
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
owned by: james
owned by: myers
member: BBa_K990002
member: BBa_F1610
member: virA
member: BBa_K574004
member: BBa_I13015
member: BBa_K574005
member: BBa_K786003
member: QS->RFP
member: BBa_K1343016
member: BBa_K1690000
member: BBa_K417007
member: BBa_K786001
member: BBa_F1780
member: BBa_K786004
member: BBa_F1611
member: sinI
member: BBa_K772005
member: BBa_F1700
member: BBa_K574006
member: BBa_K786002
member: BBa_F1760
member: BBa_K417006

virA
type: ComponentDefinition
persistent identity: BBa K389010
display id: BBa_K389010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: VirA receptor (expressed)
creator: Jonas Aretz
created: 2010-08-09T11:00:00Z
modified: 2015-05-08T01:12:19Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _517_
m user id: 0
owner id: 6670
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This BioBrick contains a ''virA'' receptor from ''Agrobacterium tumefaciens'' C58 under the control of a constitutive promotor. We used it to mutate ''virA'' by error-prone PCR. For screening the mutants BBa_K389011 is also needed.
mutable notes: The ''virA'' gene is under the control a medium strong constitutive promotor.
mutable provenance: ''A. tumefaciens'' C58, partsregistry
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_J23110
component: BBa_B0034
component: BBa_K389001
sequence annotation: BBa_B0034
sequence annotation: BBa_J23110
sequence annotation: BBa_K389001
sequence: https://synbiohub.org/public/igem/BBa_K389010_sequence/1

BBa_J23110
type: Component
persistent identity: component2219527
display id: component2219527
version: 1
top level: virA
access: public
definition: https://synbiohub.org/public/igem/BBa_J23110/1

BBa_B0034
type: Component
persistent identity: component2219529
display id: component2219529
version: 1
top level: virA
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K389001
type: Component
persistent identity: component2219533
display id: component2219533
version: 1
top level: virA
access: public
definition: https://synbiohub.org/public/igem/BBa_K389001/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2219529
display id: annotation2219529
version: 1
top level: virA
location: https://synbiohub.org/public/igem/BBa_K389010/annotation2219529/range2219529/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K389010/annotation2219529/range2219529/1
type: Range
persistent identity: range2219529
display id: range2219529
version: 1
direction: direction/reverse
top level: virA
start: 44
end: 55
orientation: inline

BBa_J23110
type: SequenceAnnotation
persistent identity: annotation2219527
display id: annotation2219527
version: 1
top level: virA
location: https://synbiohub.org/public/igem/BBa_K389010/annotation2219527/range2219527/1
component: BBa_J23110

https://synbiohub.org/public/igem/BBa_K389010/annotation2219527/range2219527/1
type: Range
persistent identity: range2219527
display id: range2219527
version: 1
direction: direction/reverse
top level: virA
start: 1
end: 35
orientation: inline

BBa_K389001
type: SequenceAnnotation
persistent identity: annotation2219533
display id: annotation2219533
version: 1
top level: virA
location: https://synbiohub.org/public/igem/BBa_K389010/annotation2219533/range2219533/1
component: BBa_K389001

https://synbiohub.org/public/igem/BBa_K389010/annotation2219533/range2219533/1
type: Range
persistent identity: range2219533
display id: range2219533
version: 1
direction: direction/reverse
top level: virA
start: 62
end: 2566
orientation: inline

BBa_K990002
type: ComponentDefinition
persistent identity: BBa K990002
display id: BBa_K990002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: IPTG Inducible LasI Device
creator: Fatma Betul CEVIK
created: 2013-06-20T11:00:00Z
modified: 2015-05-08T01:13:50Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1287_
m user id: 0
owner id: 11713
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: IPTG inducible AHL generator, LasI device.
mutable notes: Some sequence was checked NCBI
mutable provenance: pConstitutive: J23100 LasI device: K772005
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K772005
component: BBa_J23100
sequence annotation: BBa_K772005
sequence annotation: BBa_J23100
sequence: https://synbiohub.org/public/igem/BBa_K990002_sequence/1

BBa_K772005
type: Component
persistent identity: component2329865
display id: component2329865
version: 1
top level: BBa_K990002
access: public
definition: BBa_K772005

BBa_J23100
type: Component
persistent identity: component2329859
display id: component2329859
version: 1
top level: BBa_K990002
access: public
definition: https://synbiohub.org/public/igem/BBa_J23100/1

BBa_K772005
type: SequenceAnnotation
persistent identity: annotation2329865
display id: annotation2329865
version: 1
top level: BBa_K990002
location: https://synbiohub.org/public/igem/BBa_K990002/annotation2329865/range2329865/1
component: BBa_K772005

https://synbiohub.org/public/igem/BBa_K990002/annotation2329865/range2329865/1
type: Range
persistent identity: range2329865
display id: range2329865
version: 1
direction: direction/reverse
top level: BBa_K990002
start: 44
end: 2141
orientation: inline

BBa_J23100
type: SequenceAnnotation
persistent identity: annotation2329859
display id: annotation2329859
version: 1
top level: BBa_K990002
location: https://synbiohub.org/public/igem/BBa_K990002/annotation2329859/range2329859/1
component: BBa_J23100

https://synbiohub.org/public/igem/BBa_K990002/annotation2329859/range2329859/1
type: Range
persistent identity: range2329859
display id: range2329859
version: 1
direction: direction/reverse
top level: BBa_K990002
start: 1
end: 35
orientation: inline

BBa_I13015
type: ComponentDefinition
persistent identity: BBa I13015
display id: BBa_I13015
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: 3OC6HSL Sender controlled by pBad
creator: Debra, Fred, Jay, Madeleine, Cell-Cell Signaling SMUG
created: 2004-07-07T11:00:00Z
modified: 2015-08-31T04:07:32Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _6_
m user id: 0
owner id: 101
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: The pBad promoter controlling a LuxI sender device. The protein is an untagged LuxI enzyme which produces an AHL (3OC6HSL).
mutable notes: The pictures below are from a time course, where pRCV-3 produced GFP via HSL-bound LuxR promotion. I13015 was on the left half of the slide (induced in .1% arabinose, thereby producing the HSL for pRCV-3), while pRCV-3 was on the right half. Controls were done with pRCV-3 alone, I13015 alone and the two together without any arabinose. In all three cases little or no GFP was expressed.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Signalling
role: SO:0000804
component: BBa_C0061
component: BBa_B0012
component: BBa_B0034
component: BBa_B0010
component: BBa_I0500
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_C0061
sequence annotation: BBa_B0034
sequence annotation: BBa_I0500
sequence: https://synbiohub.org/public/igem/BBa_I13015_sequence/1

BBa_C0061
type: Component
persistent identity: component946929
display id: component946929
version: 1
top level: BBa_I13015
access: public
definition: https://synbiohub.org/public/igem/BBa_C0061/1

BBa_B0012
type: Component
persistent identity: component946946
display id: component946946
version: 1
top level: BBa_I13015
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0034
type: Component
persistent identity: component946919
display id: component946919
version: 1
top level: BBa_I13015
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0010
type: Component
persistent identity: component946936
display id: component946936
version: 1
top level: BBa_I13015
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_I0500
type: Component
persistent identity: component946914
display id: component946914
version: 1
top level: BBa_I13015
access: public
definition: https://synbiohub.org/public/igem/BBa_I0500/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation946936
display id: annotation946936
version: 1
top level: BBa_I13015
location: https://synbiohub.org/public/igem/BBa_I13015/annotation946936/range946936/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I13015/annotation946936/range946936/1
type: Range
persistent identity: range946936
display id: range946936
version: 1
direction: direction/forward
top level: BBa_I13015
start: 1888
end: 1967
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation946946
display id: annotation946946
version: 1
top level: BBa_I13015
location: https://synbiohub.org/public/igem/BBa_I13015/annotation946946/range946946/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I13015/annotation946946/range946946/1
type: Range
persistent identity: range946946
display id: range946946
version: 1
direction: direction/forward
top level: BBa_I13015
start: 1976
end: 2016
orientation: inline

BBa_C0061
type: SequenceAnnotation
persistent identity: annotation946929
display id: annotation946929
version: 1
top level: BBa_I13015
location: https://synbiohub.org/public/igem/BBa_I13015/annotation946929/range946929/1
component: BBa_C0061

https://synbiohub.org/public/igem/BBa_I13015/annotation946929/range946929/1
type: Range
persistent identity: range946929
display id: range946929
version: 1
direction: direction/forward
top level: BBa_I13015
start: 1237
end: 1854
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation946919
display id: annotation946919
version: 1
top level: BBa_I13015
location: https://synbiohub.org/public/igem/BBa_I13015/annotation946919/range946919/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I13015/annotation946919/range946919/1
type: Range
persistent identity: range946919
display id: range946919
version: 1
direction: direction/forward
top level: BBa_I13015
start: 1219
end: 1230
orientation: inline

BBa_I0500
type: SequenceAnnotation
persistent identity: annotation946914
display id: annotation946914
version: 1
top level: BBa_I13015
location: https://synbiohub.org/public/igem/BBa_I13015/annotation946914/range946914/1
component: BBa_I0500

https://synbiohub.org/public/igem/BBa_I13015/annotation946914/range946914/1
type: Range
persistent identity: range946914
display id: range946914
version: 1
direction: direction/forward
top level: BBa_I13015
start: 1
end: 1210
orientation: inline

BBa_K417006
type: ComponentDefinition
persistent identity: BBa K417006
display id: BBa_K417006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LacI ethylene generator
creator: Benjamin Porebski
created: 2010-10-25T11:00:00Z
modified: 2015-05-08T01:12:28Z
discontinued: false
dominant: false
group u list: _537_
m user id: 0
owner id: 6513
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: LacI regulated ethylene generator that uses 3 enzymes, blah
mutable notes: Nil
mutable provenance: E. coli genomic DNA, Apple and Tomato
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_K417005
component: BBa_K417004
component: BBa_R0010
component: BBa_B0015
component: BBa_K417003
sequence annotation: BBa_R0010
sequence annotation: BBa_K417005
sequence annotation: BBa_B0015
sequence annotation: BBa_K417004
sequence annotation: BBa_K417003
sequence: https://synbiohub.org/public/igem/BBa_K417006_sequence/1

BBa_K417005
type: Component
persistent identity: component2101921
display id: component2101921
version: 1
top level: BBa_K417006
access: public
definition: https://synbiohub.org/public/igem/BBa_K417005/1

BBa_K417004
type: Component
persistent identity: component2101914
display id: component2101914
version: 1
top level: BBa_K417006
access: public
definition: https://synbiohub.org/public/igem/BBa_K417004/1

BBa_R0010
type: Component
persistent identity: component2101895
display id: component2101895
version: 1
top level: BBa_K417006
access: public
definition: https://synbiohub.org/public/igem/BBa_R0010/1

BBa_B0015
type: Component
persistent identity: component2101928
display id: component2101928
version: 1
top level: BBa_K417006
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_K417003
type: Component
persistent identity: component2101907
display id: component2101907
version: 1
top level: BBa_K417006
access: public
definition: https://synbiohub.org/public/igem/BBa_K417003/1

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation2101895
display id: annotation2101895
version: 1
top level: BBa_K417006
location: https://synbiohub.org/public/igem/BBa_K417006/annotation2101895/range2101895/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_K417006/annotation2101895/range2101895/1
type: Range
persistent identity: range2101895
display id: range2101895
version: 1
direction: direction/forward
top level: BBa_K417006
start: 1
end: 200
orientation: inline

BBa_K417005
type: SequenceAnnotation
persistent identity: annotation2101921
display id: annotation2101921
version: 1
top level: BBa_K417006
location: https://synbiohub.org/public/igem/BBa_K417006/annotation2101921/range2101921/1
component: BBa_K417005

https://synbiohub.org/public/igem/BBa_K417006/annotation2101921/range2101921/1
type: Range
persistent identity: range2101921
display id: range2101921
version: 1
direction: direction/reverse
top level: BBa_K417006
start: 2706
end: 3671
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2101928
display id: annotation2101928
version: 1
top level: BBa_K417006
location: https://synbiohub.org/public/igem/BBa_K417006/annotation2101928/range2101928/1
component: BBa_B0015

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type: Range
persistent identity: range2101928
display id: range2101928
version: 1
direction: direction/reverse
top level: BBa_K417006
start: 3680
end: 3808
orientation: inline

BBa_K417004
type: SequenceAnnotation
persistent identity: annotation2101914
display id: annotation2101914
version: 1
top level: BBa_K417006
location: https://synbiohub.org/public/igem/BBa_K417006/annotation2101914/range2101914/1
component: BBa_K417004

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type: Range
persistent identity: range2101914
display id: range2101914
version: 1
direction: direction/reverse
top level: BBa_K417006
start: 1390
end: 2697
orientation: inline

BBa_K417003
type: SequenceAnnotation
persistent identity: annotation2101907
display id: annotation2101907
version: 1
top level: BBa_K417006
location: https://synbiohub.org/public/igem/BBa_K417006/annotation2101907/range2101907/1
component: BBa_K417003

https://synbiohub.org/public/igem/BBa_K417006/annotation2101907/range2101907/1
type: Range
persistent identity: range2101907
display id: range2101907
version: 1
direction: direction/reverse
top level: BBa_K417006
start: 209
end: 1381
orientation: inline

BBa_K417007
type: ComponentDefinition
persistent identity: BBa K417007
display id: BBa_K417007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Simple inducible (LacI) ethylene generator
creator: Ben Porebski, Andrew Perry
created: 2010-10-25T11:00:00Z
modified: 2015-05-08T01:12:28Z
discontinued: false
dominant: false
experience: experience/None
group u list: _537_
m user id: 0
owner id: 6167
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: A simpler version of BBa_K417006 which expresses ACC synthase and ACC oxidase (EFE) to produce ethylene. This version does not express the SAM synthetase enzyme (BBa_K417000) and relies on S-adenosyl methionine generated by the chassis.
mutable notes: Native LacI promoter (BBa_R0010) was chosen since it facilitates easier assembly compared with the shorter alternative BBa_R0011, although it would be worth testing both these promoters. It is unknown if the order of the coding sequences is important in this device.
mutable provenance: See part documentation for enzyme coding sequences BBa_K417001 and BBa_K417002 for details on the origin of the sequences.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_K417001
component: BBa_B0034
component: BBa_B0012
component: BBa_B0010
component: BBa_K417002
component: BBa_R0010
sequence annotation: BBa_B0034
sequence annotation: BBa_R0010
sequence annotation: BBa_K417001
sequence annotation: BBa_B0012
sequence annotation: BBa_K417002
sequence annotation: BBa_B0010
sequence: https://synbiohub.org/public/igem/BBa_K417007_sequence/1

BBa_K417001
type: Component
persistent identity: component2103054
display id: component2103054
version: 1
top level: BBa_K417007
access: public
definition: https://synbiohub.org/public/igem/BBa_K417001/1

BBa_B0034
type: Component
persistent identity: component2103050
display id: component2103050
version: 1
top level: BBa_K417007
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0034
type: Component
persistent identity: component2103056
display id: component2103056
version: 1
top level: BBa_K417007
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0012
type: Component
persistent identity: component2103063
display id: component2103063
version: 1
top level: BBa_K417007
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0010
type: Component
persistent identity: component2103061
display id: component2103061
version: 1
top level: BBa_K417007
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_K417002
type: Component
persistent identity: component2103060
display id: component2103060
version: 1
top level: BBa_K417007
access: public
definition: https://synbiohub.org/public/igem/BBa_K417002/1

BBa_R0010
type: Component
persistent identity: component2103042
display id: component2103042
version: 1
top level: BBa_K417007
access: public
definition: https://synbiohub.org/public/igem/BBa_R0010/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2103050
display id: annotation2103050
version: 1
top level: BBa_K417007
location: https://synbiohub.org/public/igem/BBa_K417007/annotation2103050/range2103050/1
component: BBa_B0034

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type: Range
persistent identity: range2103050
display id: range2103050
version: 1
direction: direction/reverse
top level: BBa_K417007
start: 209
end: 220
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation2103042
display id: annotation2103042
version: 1
top level: BBa_K417007
location: https://synbiohub.org/public/igem/BBa_K417007/annotation2103042/range2103042/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_K417007/annotation2103042/range2103042/1
type: Range
persistent identity: range2103042
display id: range2103042
version: 1
direction: direction/forward
top level: BBa_K417007
start: 1
end: 200
orientation: inline

BBa_K417001
type: SequenceAnnotation
persistent identity: annotation2103054
display id: annotation2103054
version: 1
top level: BBa_K417007
location: https://synbiohub.org/public/igem/BBa_K417007/annotation2103054/range2103054/1
component: BBa_K417001

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type: Range
persistent identity: range2103054
display id: range2103054
version: 1
direction: direction/reverse
top level: BBa_K417007
start: 227
end: 1516
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2103056
display id: annotation2103056
version: 1
top level: BBa_K417007
location: https://synbiohub.org/public/igem/BBa_K417007/annotation2103056/range2103056/1
component: BBa_B0034

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type: Range
persistent identity: range2103056
display id: range2103056
version: 1
direction: direction/reverse
top level: BBa_K417007
start: 1525
end: 1536
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2103063
display id: annotation2103063
version: 1
top level: BBa_K417007
location: https://synbiohub.org/public/igem/BBa_K417007/annotation2103063/range2103063/1
component: BBa_B0012

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type: Range
persistent identity: range2103063
display id: range2103063
version: 1
direction: direction/forward
top level: BBa_K417007
start: 2587
end: 2627
orientation: inline

BBa_K417002
type: SequenceAnnotation
persistent identity: annotation2103060
display id: annotation2103060
version: 1
top level: BBa_K417007
location: https://synbiohub.org/public/igem/BBa_K417007/annotation2103060/range2103060/1
component: BBa_K417002

https://synbiohub.org/public/igem/BBa_K417007/annotation2103060/range2103060/1
type: Range
persistent identity: range2103060
display id: range2103060
version: 1
direction: direction/reverse
top level: BBa_K417007
start: 1543
end: 2490
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2103061
display id: annotation2103061
version: 1
top level: BBa_K417007
location: https://synbiohub.org/public/igem/BBa_K417007/annotation2103061/range2103061/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K417007/annotation2103061/range2103061/1
type: Range
persistent identity: range2103061
display id: range2103061
version: 1
direction: direction/forward
top level: BBa_K417007
start: 2499
end: 2578
orientation: inline

BBa_K772005
type: ComponentDefinition
persistent identity: BBa K772005
display id: BBa_K772005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: AHL generator: LasI
creator: Mustafa Elitok
created: 2012-09-24T11:00:00Z
modified: 2015-05-08T01:13:15Z
discontinued: false
dominant: false
group u list: _1024_
m user id: 0
owner id: 9573
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Quorum sensing is the mechanism of communication between bacteria via synthesizing and detecting chemical compounds in the media. With these mediators, the promoters and their yield can be tuned or regulated. This part represents an alternative quorum sensing sender device which is designed to induce PoPS production in the parts including pLas such as BBa_K772004. LasI is an enzyme generator gene sequences which synthesizes 6HSLO3 (AHL) in order to induce a PoPS response in the pLas promoter. pLas promoter works in the presence of both 6HSLO3 and LasR protein.
mutable notes: Note that there is an RBS sequence just before LasI; in order to be easily used with quorum sensing receiver devices such as BBa_F2620 or BBa_K772004.
mutable provenance: LasI sequence is gathered from Registry.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: RBS
sequence annotation: LacI
sequence annotation: pLac
sequence annotation: Terminator
sequence annotation: LasI
sequence: https://synbiohub.org/public/igem/BBa_K772005_sequence/1

RBS
type: SequenceAnnotation
persistent identity: annotation2203714
display id: annotation2203714
version: 1
top level: BBa_K772005
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K772005/annotation2203714/range2203714/1

https://synbiohub.org/public/igem/BBa_K772005/annotation2203714/range2203714/1
type: Range
persistent identity: range2203714
display id: range2203714
version: 1
direction: direction/forward
top level: BBa_K772005
start: 1332
end: 1352
orientation: inline

LacI
type: SequenceAnnotation
persistent identity: annotation2203712
display id: annotation2203712
version: 1
top level: BBa_K772005
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K772005/annotation2203712/range2203712/1

https://synbiohub.org/public/igem/BBa_K772005/annotation2203712/range2203712/1
type: Range
persistent identity: range2203712
display id: range2203712
version: 1
direction: direction/forward
top level: BBa_K772005
start: 1
end: 1132
orientation: inline

pLac
type: SequenceAnnotation
persistent identity: annotation2203713
display id: annotation2203713
version: 1
top level: BBa_K772005
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K772005/annotation2203713/range2203713/1

https://synbiohub.org/public/igem/BBa_K772005/annotation2203713/range2203713/1
type: Range
persistent identity: range2203713
display id: range2203713
version: 1
direction: direction/forward
top level: BBa_K772005
start: 1133
end: 1331
orientation: inline

Terminator
type: SequenceAnnotation
persistent identity: annotation2203715
display id: annotation2203715
version: 1
top level: BBa_K772005
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K772005/annotation2203715/range2203715/1

https://synbiohub.org/public/igem/BBa_K772005/annotation2203715/range2203715/1
type: Range
persistent identity: range2203715
display id: range2203715
version: 1
direction: direction/forward
top level: BBa_K772005
start: 1969
end: 2098
orientation: inline

LasI
type: SequenceAnnotation
persistent identity: annotation2329745
display id: annotation2329745
version: 1
top level: BBa_K772005
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K772005/annotation2329745/range2329745/1

https://synbiohub.org/public/igem/BBa_K772005/annotation2329745/range2329745/1
type: Range
persistent identity: range2329745
display id: range2329745
version: 1
direction: direction/forward
top level: BBa_K772005
start: 1353
end: 1968
orientation: inline

BBa_K786004
type: ComponentDefinition
persistent identity: BBa K786004
display id: BBa_K786004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Red light triggering phototaxis using chp8
creator: Leung Wai Tak
created: 2012-09-20T11:00:00Z
modified: 2015-05-08T01:13:22Z
discontinued: true
dominant: false
experience: experience/None
group u list: _1041_
m user id: 0
owner id: 14285
owning group id: 9
sample status: Discontinued
status: status/Deleted
bookmark: false
mutable description: Red light triggering phototaxis using chp8, which can sense the red light from 600-700 nm.
mutable notes: 6 poly-His tag was positioned at C-terminal of the protein.
mutable provenance: The sequence of chp8 is from previous biobrick.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
sequence annotation: Constitutive promoter BBa_J23100
sequence annotation: chp8 protein
sequence annotation: RBS 1.0
sequence annotation: poly-HIS tag
sequence: https://synbiohub.org/public/igem/BBa_K786004_sequence/1

Constitutive promoter BBa_J23100
type: SequenceAnnotation
persistent identity: annotation2190187
display id: annotation2190187
version: 1
top level: BBa_K786004
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K786004/annotation2190187/range2190187/1

https://synbiohub.org/public/igem/BBa_K786004/annotation2190187/range2190187/1
type: Range
persistent identity: range2190187
display id: range2190187
version: 1
direction: direction/forward
top level: BBa_K786004
start: 1
end: 50
orientation: inline

chp8 protein
type: SequenceAnnotation
persistent identity: annotation2190189
display id: annotation2190189
version: 1
top level: BBa_K786004
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K786004/annotation2190189/range2190189/1

https://synbiohub.org/public/igem/BBa_K786004/annotation2190189/range2190189/1
type: Range
persistent identity: range2190189
display id: range2190189
version: 1
direction: direction/forward
top level: BBa_K786004
start: 69
end: 2321
orientation: inline

RBS 1.0
type: SequenceAnnotation
persistent identity: annotation2190188
display id: annotation2190188
version: 1
top level: BBa_K786004
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K786004/annotation2190188/range2190188/1

https://synbiohub.org/public/igem/BBa_K786004/annotation2190188/range2190188/1
type: Range
persistent identity: range2190188
display id: range2190188
version: 1
direction: direction/forward
top level: BBa_K786004
start: 51
end: 62
orientation: inline

poly-HIS tag
type: SequenceAnnotation
persistent identity: annotation2190190
display id: annotation2190190
version: 1
top level: BBa_K786004
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K786004/annotation2190190/range2190190/1

https://synbiohub.org/public/igem/BBa_K786004/annotation2190190/range2190190/1
type: Range
persistent identity: range2190190
display id: range2190190
version: 1
direction: direction/forward
top level: BBa_K786004
start: 2301
end: 2321
orientation: inline

BBa_K1690000
type: ComponentDefinition
persistent identity: BBa K1690000
display id: BBa_K1690000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Mating pheromone (S. cerevisiae)
creator: Anna Sosnova, Pavel Zach
created: 2015-09-15T11:00:00Z
modified: 2015-09-17T05:33:39Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2108_
m user id: 25090
owner id: 25090
owning group id: 9
status: status/Planning
bookmark: false
mutable description: This is a coding sequence of S. cerevisiae mating pheromone together with a secretion tag. Expression in the yeast cell causes secretion of the mature mating pheromone into the medium.
mutable notes: In the genome, the secretion tag is followed by a three copies of the coding sequence for the actual peptide. We had to amplify only the secretion tag first, and then added the DNA sequence of the peptide using different reverse primer.
mutable provenance: Obtained from the genome of S. cerevisiae [MF(ALPHA)1 locus].
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Kozak
sequence annotation: start
sequence annotation: Pheromone
sequence annotation: stop
sequence annotation: Secretion tag
sequence: https://synbiohub.org/public/igem/BBa_K1690000_sequence/1

Kozak
type: SequenceAnnotation
persistent identity: annotation2461253
display id: annotation2461253
version: 1
top level: BBa_K1690000
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1690000/annotation2461253/range2461253/1

https://synbiohub.org/public/igem/BBa_K1690000/annotation2461253/range2461253/1
type: Range
persistent identity: range2461253
display id: range2461253
version: 1
direction: direction/forward
top level: BBa_K1690000
start: 1
end: 6
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2461250
display id: annotation2461250
version: 1
top level: BBa_K1690000
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1690000/annotation2461250/range2461250/1

https://synbiohub.org/public/igem/BBa_K1690000/annotation2461250/range2461250/1
type: Range
persistent identity: range2461250
display id: range2461250
version: 1
direction: direction/forward
top level: BBa_K1690000
start: 7
end: 9
orientation: inline

Pheromone
type: SequenceAnnotation
persistent identity: annotation2461259
display id: annotation2461259
version: 1
top level: BBa_K1690000
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1690000/annotation2461259/range2461259/1

https://synbiohub.org/public/igem/BBa_K1690000/annotation2461259/range2461259/1
type: Range
persistent identity: range2461259
display id: range2461259
version: 1
direction: direction/forward
top level: BBa_K1690000
start: 275
end: 312
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2461256
display id: annotation2461256
version: 1
top level: BBa_K1690000
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1690000/annotation2461256/range2461256/1

https://synbiohub.org/public/igem/BBa_K1690000/annotation2461256/range2461256/1
type: Range
persistent identity: range2461256
display id: range2461256
version: 1
direction: direction/forward
top level: BBa_K1690000
start: 313
end: 315
orientation: inline

Secretion tag
type: SequenceAnnotation
persistent identity: annotation2461258
display id: annotation2461258
version: 1
top level: BBa_K1690000
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1690000/annotation2461258/range2461258/1

https://synbiohub.org/public/igem/BBa_K1690000/annotation2461258/range2461258/1
type: Range
persistent identity: range2461258
display id: range2461258
version: 1
direction: direction/forward
top level: BBa_K1690000
start: 10
end: 274
orientation: inline

BBa_F1760
type: ComponentDefinition
persistent identity: BBa F1760
display id: BBa_F1760
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: 3OC14HSL Sender Device
creator: mit
created: 2004-07-12T11:00:00Z
modified: 2015-08-31T04:07:27Z
discontinued: false
dominant: false
experience: experience/None
group u list: _6_
m user id: 0
owner id: 101
owning group id: 7
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Sender device which accepts PoPS input and produces an enzyme CinI. This enzyme produces the 3OC14HSL signaling molecule.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Signalling
role: SO:0000804
component: BBa_B0012
component: BBa_B0010
component: BBa_B0034
component: BBa_C0076
sequence annotation: BBa_B0010
sequence annotation: BBa_C0076
sequence annotation: BBa_B0012
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_F1760_sequence/1

BBa_B0012
type: Component
persistent identity: component943362
display id: component943362
version: 1
top level: BBa_F1760
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0010
type: Component
persistent identity: component943352
display id: component943352
version: 1
top level: BBa_F1760
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0034
type: Component
persistent identity: component943336
display id: component943336
version: 1
top level: BBa_F1760
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_C0076
type: Component
persistent identity: component943346
display id: component943346
version: 1
top level: BBa_F1760
access: public
definition: https://synbiohub.org/public/igem/BBa_C0076/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation943352
display id: annotation943352
version: 1
top level: BBa_F1760
location: https://synbiohub.org/public/igem/BBa_F1760/annotation943352/range943352/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_F1760/annotation943352/range943352/1
type: Range
persistent identity: range943352
display id: range943352
version: 1
direction: direction/forward
top level: BBa_F1760
start: 754
end: 833
orientation: inline

BBa_C0076
type: SequenceAnnotation
persistent identity: annotation943346
display id: annotation943346
version: 1
top level: BBa_F1760
location: https://synbiohub.org/public/igem/BBa_F1760/annotation943346/range943346/1
component: BBa_C0076

https://synbiohub.org/public/igem/BBa_F1760/annotation943346/range943346/1
type: Range
persistent identity: range943346
display id: range943346
version: 1
direction: direction/forward
top level: BBa_F1760
start: 19
end: 720
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation943362
display id: annotation943362
version: 1
top level: BBa_F1760
location: https://synbiohub.org/public/igem/BBa_F1760/annotation943362/range943362/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_F1760/annotation943362/range943362/1
type: Range
persistent identity: range943362
display id: range943362
version: 1
direction: direction/forward
top level: BBa_F1760
start: 842
end: 882
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation943336
display id: annotation943336
version: 1
top level: BBa_F1760
location: https://synbiohub.org/public/igem/BBa_F1760/annotation943336/range943336/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_F1760/annotation943336/range943336/1
type: Range
persistent identity: range943336
display id: range943336
version: 1
direction: direction/forward
top level: BBa_F1760
start: 1
end: 12
orientation: inline

BBa_K786003
type: ComponentDefinition
persistent identity: BBa K786003
display id: BBa_K786003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Sensory rhodopsin I (SRI) with HtrII & Tar, sensitive to orange light
creator: Leung Wai Tak
created: 2012-09-20T11:00:00Z
modified: 2015-05-08T01:13:22Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1041_
m user id: 0
owner id: 14285
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: Sensory rhodopsin I fusion with HtrII & Tar, which is sensitive to orange light, with sensing spectra covering from 590-650 nm. We used it for sensing blur-green light to achieve phototaxis and switch for downstream gene expression.
mutable notes: Pfam (version 26.0) was used to predict the domain of fusion protein. Linker was refereed and modified from previous research study of SRI fusion with HtrII.
mutable provenance: From genomic sequence of bacterial N. pharaonis (DSM 2160) and E.coli K12.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
sequence annotation: RBS 1.0
sequence annotation: B0015
sequence annotation: Constitutive promoter BBa_J23100
sequence annotation: SRI-HtrI-Tar
sequence: https://synbiohub.org/public/igem/BBa_K786003_sequence/1

RBS 1.0
type: SequenceAnnotation
persistent identity: annotation2190186
display id: annotation2190186
version: 1
top level: BBa_K786003
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K786003/annotation2190186/range2190186/1

https://synbiohub.org/public/igem/BBa_K786003/annotation2190186/range2190186/1
type: Range
persistent identity: range2190186
display id: range2190186
version: 1
direction: direction/forward
top level: BBa_K786003
start: 42
end: 61
orientation: inline

B0015
type: SequenceAnnotation
persistent identity: annotation2199719
display id: annotation2199719
version: 1
top level: BBa_K786003
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K786003/annotation2199719/range2199719/1

https://synbiohub.org/public/igem/BBa_K786003/annotation2199719/range2199719/1
type: Range
persistent identity: range2199719
display id: range2199719
version: 1
direction: direction/forward
top level: BBa_K786003
start: 2029
end: 2158
orientation: inline

Constitutive promoter BBa_J23100
type: SequenceAnnotation
persistent identity: annotation2190185
display id: annotation2190185
version: 1
top level: BBa_K786003
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K786003/annotation2190185/range2190185/1

https://synbiohub.org/public/igem/BBa_K786003/annotation2190185/range2190185/1
type: Range
persistent identity: range2190185
display id: range2190185
version: 1
direction: direction/forward
top level: BBa_K786003
start: 1
end: 41
orientation: inline

SRI-HtrI-Tar
type: SequenceAnnotation
persistent identity: annotation2199706
display id: annotation2199706
version: 1
top level: BBa_K786003
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K786003/annotation2199706/range2199706/1

https://synbiohub.org/public/igem/BBa_K786003/annotation2199706/range2199706/1
type: Range
persistent identity: range2199706
display id: range2199706
version: 1
direction: direction/forward
top level: BBa_K786003
start: 68
end: 1992
orientation: inline

BBa_F1610
type: ComponentDefinition
persistent identity: BBa F1610
display id: BBa_F1610
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: 3OC6HSL Sender Device
creator: Caitlin Conboy and Jennifer Braff
created: 2003-12-04T12:00:00Z
modified: 2015-08-31T04:07:27Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This device accepts PoPS as input and produces the LuxI enzyme. This enzyme produces 3OC6HSL.
mutable provenance: Formerly I0461
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Signalling
role: SO:0000804
component: BBa_B0012
component: BBa_B0034
component: BBa_C0061
component: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_B0034
sequence annotation: BBa_C0061
sequence annotation: BBa_B0010
sequence: https://synbiohub.org/public/igem/BBa_F1610_sequence/1

BBa_B0012
type: Component
persistent identity: component943160
display id: component943160
version: 1
top level: BBa_F1610
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0034
type: Component
persistent identity: component943133
display id: component943133
version: 1
top level: BBa_F1610
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_C0061
type: Component
persistent identity: component943143
display id: component943143
version: 1
top level: BBa_F1610
access: public
definition: https://synbiohub.org/public/igem/BBa_C0061/1

BBa_B0010
type: Component
persistent identity: component943150
display id: component943150
version: 1
top level: BBa_F1610
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation943160
display id: annotation943160
version: 1
top level: BBa_F1610
location: https://synbiohub.org/public/igem/BBa_F1610/annotation943160/range943160/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_F1610/annotation943160/range943160/1
type: Range
persistent identity: range943160
display id: range943160
version: 1
direction: direction/forward
top level: BBa_F1610
start: 758
end: 798
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation943133
display id: annotation943133
version: 1
top level: BBa_F1610
location: https://synbiohub.org/public/igem/BBa_F1610/annotation943133/range943133/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_F1610/annotation943133/range943133/1
type: Range
persistent identity: range943133
display id: range943133
version: 1
direction: direction/forward
top level: BBa_F1610
start: 1
end: 12
orientation: inline

BBa_C0061
type: SequenceAnnotation
persistent identity: annotation943143
display id: annotation943143
version: 1
top level: BBa_F1610
location: https://synbiohub.org/public/igem/BBa_F1610/annotation943143/range943143/1
component: BBa_C0061

https://synbiohub.org/public/igem/BBa_F1610/annotation943143/range943143/1
type: Range
persistent identity: range943143
display id: range943143
version: 1
direction: direction/forward
top level: BBa_F1610
start: 19
end: 636
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation943150
display id: annotation943150
version: 1
top level: BBa_F1610
location: https://synbiohub.org/public/igem/BBa_F1610/annotation943150/range943150/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_F1610/annotation943150/range943150/1
type: Range
persistent identity: range943150
display id: range943150
version: 1
direction: direction/forward
top level: BBa_F1610
start: 670
end: 749
orientation: inline

BBa_K786002
type: ComponentDefinition
persistent identity: BBa K786002
display id: BBa_K786002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Sensory rhodopsin II (SRII) with HtrII & Tar, sensitive to blue-green light
creator: Leung Wai Tak
created: 2012-09-20T11:00:00Z
modified: 2015-05-08T01:13:22Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1041_
m user id: 0
owner id: 14285
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: Sensory rhodopsin II fusion with HtrII & Tar, which is sensitive to blue-green light, with sensing spectra covering from 400-500 nm. We used it for sensing blur-green light to achieve phototaxis and switch for downstream gene expression.
mutable notes: Pfam (version 26.0) was used to predict the domain of fusion protein. Linker was refereed and modified from previous research study of SRII fusion with HtrII.
mutable provenance: From genomic sequence of bacterial N. pharaonis (DSM 2160) and E.coli K12.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
sequence annotation: RBS 1.0
sequence annotation: BamHI for C-terminal fusion
sequence annotation: Constitutive promoter BBa_J23100
sequence annotation: B0015
sequence annotation: SRII-HtrII-Tar
sequence annotation: HindIII for N-terminal fusin
sequence: https://synbiohub.org/public/igem/BBa_K786002_sequence/1

RBS 1.0
type: SequenceAnnotation
persistent identity: annotation2199690
display id: annotation2199690
version: 1
top level: BBa_K786002
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K786002/annotation2199690/range2199690/1

https://synbiohub.org/public/igem/BBa_K786002/annotation2199690/range2199690/1
type: Range
persistent identity: range2199690
display id: range2199690
version: 1
direction: direction/forward
top level: BBa_K786002
start: 42
end: 61
orientation: inline

BamHI for C-terminal fusion
type: SequenceAnnotation
persistent identity: annotation2199693
display id: annotation2199693
version: 1
top level: BBa_K786002
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K786002/annotation2199693/range2199693/1

https://synbiohub.org/public/igem/BBa_K786002/annotation2199693/range2199693/1
type: Range
persistent identity: range2199693
display id: range2199693
version: 1
direction: direction/forward
top level: BBa_K786002
start: 785
end: 790
orientation: inline

Constitutive promoter BBa_J23100
type: SequenceAnnotation
persistent identity: annotation2199689
display id: annotation2199689
version: 1
top level: BBa_K786002
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K786002/annotation2199689/range2199689/1

https://synbiohub.org/public/igem/BBa_K786002/annotation2199689/range2199689/1
type: Range
persistent identity: range2199689
display id: range2199689
version: 1
direction: direction/forward
top level: BBa_K786002
start: 1
end: 41
orientation: inline

B0015
type: SequenceAnnotation
persistent identity: annotation2199694
display id: annotation2199694
version: 1
top level: BBa_K786002
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K786002/annotation2199694/range2199694/1

https://synbiohub.org/public/igem/BBa_K786002/annotation2199694/range2199694/1
type: Range
persistent identity: range2199694
display id: range2199694
version: 1
direction: direction/forward
top level: BBa_K786002
start: 2117
end: 2245
orientation: inline

SRII-HtrII-Tar
type: SequenceAnnotation
persistent identity: annotation2199692
display id: annotation2199692
version: 1
top level: BBa_K786002
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K786002/annotation2199692/range2199692/1

https://synbiohub.org/public/igem/BBa_K786002/annotation2199692/range2199692/1
type: Range
persistent identity: range2199692
display id: range2199692
version: 1
direction: direction/forward
top level: BBa_K786002
start: 68
end: 2083
orientation: inline

HindIII for N-terminal fusin
type: SequenceAnnotation
persistent identity: annotation2199691
display id: annotation2199691
version: 1
top level: BBa_K786002
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K786002/annotation2199691/range2199691/1

https://synbiohub.org/public/igem/BBa_K786002/annotation2199691/range2199691/1
type: Range
persistent identity: range2199691
display id: range2199691
version: 1
direction: direction/forward
top level: BBa_K786002
start: 62
end: 67
orientation: inline

QS->RFP
type: ComponentDefinition
persistent identity: BBa J85120
display id: BBa_J85120
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: High Population->mRFP (pLac->LuxI; pLacIQ->luxR; pLux->mRFP;)
creator: Cory Li
created: 2010-05-31T11:00:00Z
modified: 2015-05-08T01:08:29Z
discontinued: false
dominant: false
experience: experience/None
group u list: _406_
m user id: 0
owner id: 2073
owning group id: 387
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Quorum sensing circuit under a pLac promoter. Population turns red (mRFP) once quorum sensing kicks in.
mutable notes: Double terminator not used after LuxI because it is unnecessary and has the potential to make the plasmid more unstable.
mutable provenance: Built through assembly of BBa_K084014 and BBa_J10000.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_J10000
component: BBa_K084014
sequence annotation: BBa_K084014
sequence annotation: BBa_J10000
sequence: https://synbiohub.org/public/igem/BBa_J85120_sequence/1

BBa_J10000
type: Component
persistent identity: component2294832
display id: component2294832
version: 1
top level: QS->RFP
access: public
definition: https://synbiohub.org/public/igem/BBa_J10000/1

BBa_K084014
type: Component
persistent identity: component2294800
display id: component2294800
version: 1
top level: QS->RFP
access: public
definition: https://synbiohub.org/public/igem/BBa_K084014/1

BBa_K084014
type: SequenceAnnotation
persistent identity: annotation2294800
display id: annotation2294800
version: 1
top level: QS->RFP
location: https://synbiohub.org/public/igem/BBa_J85120/annotation2294800/range2294800/1
component: BBa_K084014

https://synbiohub.org/public/igem/BBa_J85120/annotation2294800/range2294800/1
type: Range
persistent identity: range2294800
display id: range2294800
version: 1
direction: direction/reverse
top level: QS->RFP
start: 1
end: 869
orientation: inline

BBa_J10000
type: SequenceAnnotation
persistent identity: annotation2294832
display id: annotation2294832
version: 1
top level: QS->RFP
location: https://synbiohub.org/public/igem/BBa_J85120/annotation2294832/range2294832/1
component: BBa_J10000

https://synbiohub.org/public/igem/BBa_J85120/annotation2294832/range2294832/1
type: Range
persistent identity: range2294832
display id: range2294832
version: 1
direction: direction/reverse
top level: QS->RFP
start: 878
end: 2653
orientation: inline

BBa_K574004
type: ComponentDefinition
persistent identity: BBa K574004
display id: BBa_K574004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: 3OC12HSL regulated by TetR
creator: Lei Wei
created: 2011-09-28T11:00:00Z
modified: 2015-05-08T01:12:44Z
discontinued: false
dominant: false
experience: experience/None
group u list: _745_
m user id: 0
owner id: 8529
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: p(tetR) + lasI
mutable notes: Empty.
mutable provenance: Existing Parts.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Signalling
role: SO:0000804
component: BBa_R0040
component: BBa_K081016
sequence annotation: BBa_K081016
sequence annotation: BBa_R0040
sequence: https://synbiohub.org/public/igem/BBa_K574004_sequence/1

BBa_R0040
type: Component
persistent identity: component2250757
display id: component2250757
version: 1
top level: BBa_K574004
access: public
definition: https://synbiohub.org/public/igem/BBa_R0040/1

BBa_K081016
type: Component
persistent identity: component2250774
display id: component2250774
version: 1
top level: BBa_K574004
access: public
definition: https://synbiohub.org/public/igem/BBa_K081016/1

BBa_K081016
type: SequenceAnnotation
persistent identity: annotation2250774
display id: annotation2250774
version: 1
top level: BBa_K574004
location: https://synbiohub.org/public/igem/BBa_K574004/annotation2250774/range2250774/1
component: BBa_K081016

https://synbiohub.org/public/igem/BBa_K574004/annotation2250774/range2250774/1
type: Range
persistent identity: range2250774
display id: range2250774
version: 1
direction: direction/reverse
top level: BBa_K574004
start: 63
end: 797
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2250757
display id: annotation2250757
version: 1
top level: BBa_K574004
location: https://synbiohub.org/public/igem/BBa_K574004/annotation2250757/range2250757/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_K574004/annotation2250757/range2250757/1
type: Range
persistent identity: range2250757
display id: range2250757
version: 1
direction: direction/forward
top level: BBa_K574004
start: 1
end: 54
orientation: inline

BBa_K574006
type: ComponentDefinition
persistent identity: BBa K574006
display id: BBa_K574006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: 3OC12HSL regulated by pTet and pBad
creator: Lei Wei
created: 2011-09-28T11:00:00Z
modified: 2015-05-08T01:12:44Z
discontinued: false
dominant: false
experience: experience/None
group u list: _745_
m user id: 0
owner id: 8529
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: Part 2/3 of a Oscillator.
mutable notes: Empty.
mutable provenance: Existing Parts.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_K574004
component: BBa_K574005
sequence annotation: BBa_K574004
sequence annotation: BBa_K574005
sequence: https://synbiohub.org/public/igem/BBa_K574006_sequence/1

BBa_K574004
type: Component
persistent identity: component2295487
display id: component2295487
version: 1
top level: BBa_K574006
access: public
definition: BBa_K574004

BBa_K574005
type: Component
persistent identity: component2295507
display id: component2295507
version: 1
top level: BBa_K574006
access: public
definition: BBa_K574005

BBa_K574004
type: SequenceAnnotation
persistent identity: annotation2295487
display id: annotation2295487
version: 1
top level: BBa_K574006
location: https://synbiohub.org/public/igem/BBa_K574006/annotation2295487/range2295487/1
component: BBa_K574004

https://synbiohub.org/public/igem/BBa_K574006/annotation2295487/range2295487/1
type: Range
persistent identity: range2295487
display id: range2295487
version: 1
direction: direction/reverse
top level: BBa_K574006
start: 1
end: 797
orientation: inline

BBa_K574005
type: SequenceAnnotation
persistent identity: annotation2295507
display id: annotation2295507
version: 1
top level: BBa_K574006
location: https://synbiohub.org/public/igem/BBa_K574006/annotation2295507/range2295507/1
component: BBa_K574005

https://synbiohub.org/public/igem/BBa_K574006/annotation2295507/range2295507/1
type: Range
persistent identity: range2295507
display id: range2295507
version: 1
direction: direction/reverse
top level: BBa_K574006
start: 806
end: 2517
orientation: inline

BBa_K786001
type: ComponentDefinition
persistent identity: BBa K786001
display id: BBa_K786001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Sensory rhodopsin II (SRII) with HtrII & Tsr, sensitive to blue-green light
creator: Leung Wai Tak
created: 2012-09-20T11:00:00Z
modified: 2015-05-08T01:13:22Z
discontinued: false
dominant: false
group u list: _1041_
m user id: 0
owner id: 14285
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Sensory rhodopsin II fusion with HtrII & Tsr, which is sensitive to blue-green light, with sensing spectra covering from 400-500 nm. We used it for sensing blur-green light to achieve phototaxis and switch for downstream gene expression.
mutable notes: Pfam (version 26.0) was used to predict the domain of fusion protein. Linker was refereed and modified from previous research study of SRII fusion with HtrII.
mutable provenance: From genomic sequence of bacterial N. pharaonis (DSM 2160) and E.coli K12.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
sequence annotation: Constitutive promoter BBa_J23100
sequence annotation: linker
sequence annotation: HtrII
sequence annotation: RBS 1.0
sequence annotation: B0015
sequence annotation: Sensory Rhodopsin II
sequence annotation: BamHI for C-terminal fusion
sequence annotation: Tsr
sequence annotation: HindIII for N-terminal fusin
sequence: https://synbiohub.org/public/igem/BBa_K786001_sequence/1

Constitutive promoter BBa_J23100
type: SequenceAnnotation
persistent identity: annotation2199664
display id: annotation2199664
version: 1
top level: BBa_K786001
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K786001/annotation2199664/range2199664/1

https://synbiohub.org/public/igem/BBa_K786001/annotation2199664/range2199664/1
type: Range
persistent identity: range2199664
display id: range2199664
version: 1
direction: direction/forward
top level: BBa_K786001
start: 1
end: 42
orientation: inline

linker
type: SequenceAnnotation
persistent identity: annotation2199668
display id: annotation2199668
version: 1
top level: BBa_K786001
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K786001/annotation2199668/range2199668/1

https://synbiohub.org/public/igem/BBa_K786001/annotation2199668/range2199668/1
type: Range
persistent identity: range2199668
display id: range2199668
version: 1
direction: direction/forward
top level: BBa_K786001
start: 785
end: 811
orientation: inline

HtrII
type: SequenceAnnotation
persistent identity: annotation2199670
display id: annotation2199670
version: 1
top level: BBa_K786001
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K786001/annotation2199670/range2199670/1

https://synbiohub.org/public/igem/BBa_K786001/annotation2199670/range2199670/1
type: Range
persistent identity: range2199670
display id: range2199670
version: 1
direction: direction/forward
top level: BBa_K786001
start: 812
end: 1210
orientation: inline

RBS 1.0
type: SequenceAnnotation
persistent identity: annotation2199665
display id: annotation2199665
version: 1
top level: BBa_K786001
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K786001/annotation2199665/range2199665/1

https://synbiohub.org/public/igem/BBa_K786001/annotation2199665/range2199665/1
type: Range
persistent identity: range2199665
display id: range2199665
version: 1
direction: direction/forward
top level: BBa_K786001
start: 43
end: 55
orientation: inline

B0015
type: SequenceAnnotation
persistent identity: annotation2199672
display id: annotation2199672
version: 1
top level: BBa_K786001
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K786001/annotation2199672/range2199672/1

https://synbiohub.org/public/igem/BBa_K786001/annotation2199672/range2199672/1
type: Range
persistent identity: range2199672
display id: range2199672
version: 1
direction: direction/forward
top level: BBa_K786001
start: 2105
end: 2233
orientation: inline

Sensory Rhodopsin II
type: SequenceAnnotation
persistent identity: annotation2199667
display id: annotation2199667
version: 1
top level: BBa_K786001
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K786001/annotation2199667/range2199667/1

https://synbiohub.org/public/igem/BBa_K786001/annotation2199667/range2199667/1
type: Range
persistent identity: range2199667
display id: range2199667
version: 1
direction: direction/forward
top level: BBa_K786001
start: 68
end: 784
orientation: inline

BamHI for C-terminal fusion
type: SequenceAnnotation
persistent identity: annotation2199669
display id: annotation2199669
version: 1
top level: BBa_K786001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K786001/annotation2199669/range2199669/1

https://synbiohub.org/public/igem/BBa_K786001/annotation2199669/range2199669/1
type: Range
persistent identity: range2199669
display id: range2199669
version: 1
direction: direction/forward
top level: BBa_K786001
start: 785
end: 790
orientation: inline

Tsr
type: SequenceAnnotation
persistent identity: annotation2199671
display id: annotation2199671
version: 1
top level: BBa_K786001
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K786001/annotation2199671/range2199671/1

https://synbiohub.org/public/igem/BBa_K786001/annotation2199671/range2199671/1
type: Range
persistent identity: range2199671
display id: range2199671
version: 1
direction: direction/forward
top level: BBa_K786001
start: 1210
end: 2070
orientation: inline

HindIII for N-terminal fusin
type: SequenceAnnotation
persistent identity: annotation2199666
display id: annotation2199666
version: 1
top level: BBa_K786001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K786001/annotation2199666/range2199666/1

https://synbiohub.org/public/igem/BBa_K786001/annotation2199666/range2199666/1
type: Range
persistent identity: range2199666
display id: range2199666
version: 1
direction: direction/forward
top level: BBa_K786001
start: 62
end: 67
orientation: inline

BBa_F1611
type: ComponentDefinition
persistent identity: BBa F1611
display id: BBa_F1611
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: 3OC6HSL Sender Device
creator: jcbraff (page edited by BC 5/9/05)
created: 2004-08-06T11:00:00Z
modified: 2015-08-31T04:07:27Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_1_
m user id: 0
owner id: 61
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This protein generator produces the enzyme LuxI (no ssrA tag). This enzyme metabolized the small intracellular molecule, SAM, to produce 3OC6HSL.
mutable provenance: formerly I1461
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Signalling
role: SO:0000804
component: BBa_B0010
component: BBa_B0012
component: BBa_B0034
component: BBa_C0161
sequence annotation: BBa_B0010
sequence annotation: BBa_C0161
sequence annotation: BBa_B0012
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_F1611_sequence/1

BBa_B0010
type: Component
persistent identity: component1034011
display id: component1034011
version: 1
top level: BBa_F1611
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
type: Component
persistent identity: component1034021
display id: component1034021
version: 1
top level: BBa_F1611
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0034
type: Component
persistent identity: component1034004
display id: component1034004
version: 1
top level: BBa_F1611
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_C0161
type: Component
persistent identity: component1034006
display id: component1034006
version: 1
top level: BBa_F1611
access: public
definition: https://synbiohub.org/public/igem/BBa_C0161/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1034011
display id: annotation1034011
version: 1
top level: BBa_F1611
location: https://synbiohub.org/public/igem/BBa_F1611/annotation1034011/range1034011/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_F1611/annotation1034011/range1034011/1
type: Range
persistent identity: range1034011
display id: range1034011
version: 1
direction: direction/forward
top level: BBa_F1611
start: 612
end: 691
orientation: inline

BBa_C0161
type: SequenceAnnotation
persistent identity: annotation1034006
display id: annotation1034006
version: 1
top level: BBa_F1611
location: https://synbiohub.org/public/igem/BBa_F1611/annotation1034006/range1034006/1
component: BBa_C0161

https://synbiohub.org/public/igem/BBa_F1611/annotation1034006/range1034006/1
type: Range
persistent identity: range1034006
display id: range1034006
version: 1
direction: direction/forward
top level: BBa_F1611
start: 19
end: 603
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1034021
display id: annotation1034021
version: 1
top level: BBa_F1611
location: https://synbiohub.org/public/igem/BBa_F1611/annotation1034021/range1034021/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_F1611/annotation1034021/range1034021/1
type: Range
persistent identity: range1034021
display id: range1034021
version: 1
direction: direction/forward
top level: BBa_F1611
start: 700
end: 740
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1034004
display id: annotation1034004
version: 1
top level: BBa_F1611
location: https://synbiohub.org/public/igem/BBa_F1611/annotation1034004/range1034004/1
component: BBa_B0034

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type: Range
persistent identity: range1034004
display id: range1034004
version: 1
direction: direction/forward
top level: BBa_F1611
start: 1
end: 12
orientation: inline

BBa_F1700
type: ComponentDefinition
persistent identity: BBa F1700
display id: BBa_F1700
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: N-butyryl-HSL Sender Device
creator: mit
created: 2004-07-12T11:00:00Z
modified: 2015-08-31T04:07:27Z
discontinued: false
dominant: false
experience: experience/None
group u list: _6_
m user id: 0
owner id: 101
owning group id: 7
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Sender device which accepts PoPS input and produces an enzyme RhlI. This enzyme produces the N-butyryl-HSL signaling molecule.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Signalling
role: SO:0000804
component: BBa_B0034
component: BBa_B0012
component: BBa_C0070
component: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_C0070
sequence annotation: BBa_B0010
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_F1700_sequence/1

BBa_B0034
type: Component
persistent identity: component943272
display id: component943272
version: 1
top level: BBa_F1700
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0012
type: Component
persistent identity: component943298
display id: component943298
version: 1
top level: BBa_F1700
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_C0070
type: Component
persistent identity: component943282
display id: component943282
version: 1
top level: BBa_F1700
access: public
definition: https://synbiohub.org/public/igem/BBa_C0070/1

BBa_B0010
type: Component
persistent identity: component943288
display id: component943288
version: 1
top level: BBa_F1700
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation943298
display id: annotation943298
version: 1
top level: BBa_F1700
location: https://synbiohub.org/public/igem/BBa_F1700/annotation943298/range943298/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_F1700/annotation943298/range943298/1
type: Range
persistent identity: range943298
display id: range943298
version: 1
direction: direction/forward
top level: BBa_F1700
start: 782
end: 822
orientation: inline

BBa_C0070
type: SequenceAnnotation
persistent identity: annotation943282
display id: annotation943282
version: 1
top level: BBa_F1700
location: https://synbiohub.org/public/igem/BBa_F1700/annotation943282/range943282/1
component: BBa_C0070

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type: Range
persistent identity: range943282
display id: range943282
version: 1
direction: direction/forward
top level: BBa_F1700
start: 19
end: 660
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation943288
display id: annotation943288
version: 1
top level: BBa_F1700
location: https://synbiohub.org/public/igem/BBa_F1700/annotation943288/range943288/1
component: BBa_B0010

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type: Range
persistent identity: range943288
display id: range943288
version: 1
direction: direction/forward
top level: BBa_F1700
start: 694
end: 773
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation943272
display id: annotation943272
version: 1
top level: BBa_F1700
location: https://synbiohub.org/public/igem/BBa_F1700/annotation943272/range943272/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_F1700/annotation943272/range943272/1
type: Range
persistent identity: range943272
display id: range943272
version: 1
direction: direction/forward
top level: BBa_F1700
start: 1
end: 12
orientation: inline

BBa_F1780
type: ComponentDefinition
persistent identity: BBa F1780
display id: BBa_F1780
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: AI-1 Sender Device
creator: Fred, Jay, Madeline, Debra
created: 2004-07-12T11:00:00Z
modified: 2015-08-31T04:07:27Z
discontinued: false
dominant: false
experience: experience/None
group u list: _6_
m user id: 0
owner id: 101
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable description: Sender device which accepts PoPS input and produces an enzyme LasI. This enzyme produces the AI-1 signaling molecule
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Signalling
role: SO:0000804
component: BBa_B0010
component: BBa_B0034
component: BBa_C0078
component: BBa_B0012
sequence annotation: BBa_B0010
sequence annotation: BBa_C0078
sequence annotation: BBa_B0034
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_F1780_sequence/1

BBa_B0010
type: Component
persistent identity: component943221
display id: component943221
version: 1
top level: BBa_F1780
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0034
type: Component
persistent identity: component943205
display id: component943205
version: 1
top level: BBa_F1780
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_C0078
type: Component
persistent identity: component943215
display id: component943215
version: 1
top level: BBa_F1780
access: public
definition: https://synbiohub.org/public/igem/BBa_C0078/1

BBa_B0012
type: Component
persistent identity: component943231
display id: component943231
version: 1
top level: BBa_F1780
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation943221
display id: annotation943221
version: 1
top level: BBa_F1780
location: https://synbiohub.org/public/igem/BBa_F1780/annotation943221/range943221/1
component: BBa_B0010

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type: Range
persistent identity: range943221
display id: range943221
version: 1
direction: direction/forward
top level: BBa_F1780
start: 694
end: 773
orientation: inline

BBa_C0078
type: SequenceAnnotation
persistent identity: annotation943215
display id: annotation943215
version: 1
top level: BBa_F1780
location: https://synbiohub.org/public/igem/BBa_F1780/annotation943215/range943215/1
component: BBa_C0078

https://synbiohub.org/public/igem/BBa_F1780/annotation943215/range943215/1
type: Range
persistent identity: range943215
display id: range943215
version: 1
direction: direction/forward
top level: BBa_F1780
start: 19
end: 660
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation943205
display id: annotation943205
version: 1
top level: BBa_F1780
location: https://synbiohub.org/public/igem/BBa_F1780/annotation943205/range943205/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_F1780/annotation943205/range943205/1
type: Range
persistent identity: range943205
display id: range943205
version: 1
direction: direction/forward
top level: BBa_F1780
start: 1
end: 12
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation943231
display id: annotation943231
version: 1
top level: BBa_F1780
location: https://synbiohub.org/public/igem/BBa_F1780/annotation943231/range943231/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_F1780/annotation943231/range943231/1
type: Range
persistent identity: range943231
display id: range943231
version: 1
direction: direction/forward
top level: BBa_F1780
start: 782
end: 822
orientation: inline

BBa_K574005
type: ComponentDefinition
persistent identity: BBa K574005
display id: BBa_K574005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: 3OC12HSL and YFP regulated by pBad
creator: Lei Wei
created: 2011-09-28T11:00:00Z
modified: 2015-05-08T01:12:44Z
discontinued: false
dominant: false
experience: experience/None
group u list: _745_
m user id: 0
owner id: 8529
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: pBAD + lasI + yfp.
mutable notes: Empty.
mutable provenance: Exsiting Parts.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Signalling
role: SO:0000804
component: BBa_E0430
component: BBa_I13453
component: BBa_K081009
sequence annotation: BBa_I13453
sequence annotation: BBa_E0430
sequence annotation: BBa_K081009
sequence: https://synbiohub.org/public/igem/BBa_K574005_sequence/1

BBa_E0430
type: Component
persistent identity: component2268889
display id: component2268889
version: 1
top level: BBa_K574005
access: public
definition: https://synbiohub.org/public/igem/BBa_E0430/1

BBa_I13453
type: Component
persistent identity: component2268871
display id: component2268871
version: 1
top level: BBa_K574005
access: public
definition: https://synbiohub.org/public/igem/BBa_I13453/1

BBa_K081009
type: Component
persistent identity: component2268879
display id: component2268879
version: 1
top level: BBa_K574005
access: public
definition: https://synbiohub.org/public/igem/BBa_K081009/1

BBa_I13453
type: SequenceAnnotation
persistent identity: annotation2268871
display id: annotation2268871
version: 1
top level: BBa_K574005
location: https://synbiohub.org/public/igem/BBa_K574005/annotation2268871/range2268871/1
component: BBa_I13453

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type: Range
persistent identity: range2268871
display id: range2268871
version: 1
direction: direction/reverse
top level: BBa_K574005
start: 1
end: 130
orientation: inline

BBa_E0430
type: SequenceAnnotation
persistent identity: annotation2268889
display id: annotation2268889
version: 1
top level: BBa_K574005
location: https://synbiohub.org/public/igem/BBa_K574005/annotation2268889/range2268889/1
component: BBa_E0430

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type: Range
persistent identity: range2268889
display id: range2268889
version: 1
direction: direction/reverse
top level: BBa_K574005
start: 835
end: 1712
orientation: inline

BBa_K081009
type: SequenceAnnotation
persistent identity: annotation2268879
display id: annotation2268879
version: 1
top level: BBa_K574005
location: https://synbiohub.org/public/igem/BBa_K574005/annotation2268879/range2268879/1
component: BBa_K081009

https://synbiohub.org/public/igem/BBa_K574005/annotation2268879/range2268879/1
type: Range
persistent identity: range2268879
display id: range2268879
version: 1
direction: direction/reverse
top level: BBa_K574005
start: 139
end: 826
orientation: inline

sinI
type: ComponentDefinition
persistent identity: BBa K594001
display id: BBa_K594001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: a luxI homologous AHL synthetase from Sinorhizobium meliloti 1021
creator: Yu Xiaoyan
created: 2011-09-29T11:00:00Z
modified: 2015-05-08T01:12:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _765_
m user id: 0
owner id: 8221
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: The Sinorhizobium meliloti strain Rm1021 harbors at least two quorum-sensing systems; one of them is sinR/sinI system, The sinI is responsible for the production of several novel AHLs, including C12-HSL, 3-Oxo-C14-HSL, C16:1-HSL, 3-Oxo-C16:1-HSL, C18-HSL, some of theses are the longest AHLs identified so far. The sin system correlates with a delay in the appearance of nitrogen-fixing nodules, as well as with an overall decrease in the number of pink nodules, suggesting a role for quorum sensing in establishing a successful symbiosis with Medicago sativa. As a autoinducer synthatase, the use of sinI is the same with luxI, namely to produce AHLS.
mutable notes: The Sinorhizobium meliloti 1021 is a mode organism in Rhizobia. so We got the S. meliloti 1021 from Nanjing Agriculture University easily, and then cloned the sinI gene from S. meliloti 1021 with primers designed by the part design tool in the website ginkgobioworks.com.
mutable provenance: SinI is from the Sinorhizobium meliloti 1021. We get its gene sequence from the www.microbesonline.org, and then cloned them through PCR with biobrick part primers.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: sinI
sequence: https://synbiohub.org/public/igem/BBa_K594001_sequence/1

sinI
type: SequenceAnnotation
persistent identity: annotation2147997
display id: annotation2147997
version: 1
top level: sinI
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K594001/annotation2147997/range2147997/1

https://synbiohub.org/public/igem/BBa_K594001/annotation2147997/range2147997/1
type: Range
persistent identity: range2147997
display id: range2147997
version: 1
direction: direction/forward
top level: sinI
start: 1
end: 645
orientation: inline

BBa_K1343016
type: ComponentDefinition
persistent identity: BBa K1343016
display id: BBa_K1343016
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Pcat->RBS->TaZ->dTer
creator: Karen Joseph
created: 2014-10-04T11:00:00Z
modified: 2015-05-08T01:09:59Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1718_
m user id: 0
owner id: 20669
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This part generates the TaZ receptor. The TaZ is under a Pcat constitutive promoter. TaZ is a fused Tar-EnvZ receptor meant for Aspartate dependent response, by activating the EnvZ/OmpR two component signalling system and thus activating the PompC promoter. We used this part for prove-of-concept for low concentration detection. By using this TaZ Generator we were able to test different concentrations of Aspartate and their effect on the PompC promoter present in another plasmid.
mutable notes: We had to add a gggggg sequance between RBS and TaZ sequances.
mutable provenance: Pcat: BBa_I14033 RBS: BBa_B0034 TaZ: BBa_C0082 dTer: BBa_B0015
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: dTer
sequence annotation: RBS
sequence annotation: Pcat
sequence annotation: Taz
sequence: https://synbiohub.org/public/igem/BBa_K1343016_sequence/1

dTer
type: SequenceAnnotation
persistent identity: annotation2394318
display id: annotation2394318
version: 1
top level: BBa_K1343016
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1343016/annotation2394318/range2394318/1

https://synbiohub.org/public/igem/BBa_K1343016/annotation2394318/range2394318/1
type: Range
persistent identity: range2394318
display id: range2394318
version: 1
direction: direction/forward
top level: BBa_K1343016
start: 1765
end: 1893
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2394316
display id: annotation2394316
version: 1
top level: BBa_K1343016
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1343016/annotation2394316/range2394316/1

https://synbiohub.org/public/igem/BBa_K1343016/annotation2394316/range2394316/1
type: Range
persistent identity: range2394316
display id: range2394316
version: 1
direction: direction/forward
top level: BBa_K1343016
start: 39
end: 50
orientation: inline

Pcat
type: SequenceAnnotation
persistent identity: annotation2394315
display id: annotation2394315
version: 1
top level: BBa_K1343016
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1343016/annotation2394315/range2394315/1

https://synbiohub.org/public/igem/BBa_K1343016/annotation2394315/range2394315/1
type: Range
persistent identity: range2394315
display id: range2394315
version: 1
direction: direction/forward
top level: BBa_K1343016
start: 1
end: 38
orientation: inline

Taz
type: SequenceAnnotation
persistent identity: annotation2394317
display id: annotation2394317
version: 1
top level: BBa_K1343016
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1343016/annotation2394317/range2394317/1

https://synbiohub.org/public/igem/BBa_K1343016/annotation2394317/range2394317/1
type: Range
persistent identity: range2394317
display id: range2394317
version: 1
direction: direction/forward
top level: BBa_K1343016
start: 57
end: 1572
orientation: inline