Similar to part J5527 where mRFP is replaced with GFP
Tetracycline/aTc inducible promoter with GFP reporter tagged with LAA-ssrA degradation tag followed by terminator. The tagged GFP is actively degraded within cell, thus provides better temporal resolution of green fluorescence and promoter activity.
Part was assembled using BrickClip assembly (BBF RFC104) using other biobrick parts as templates. BrickClip assembly is a special case of more general Paperclip assembly method.[1]
POPS+RIPS generates CFP. POPS generates YFP
Lambda-system Pr promoter with RFP reporter
Testing Pr base level activity with specific reporter RFP.
For more details see Sensing Device and Event Processing Device in the ETH Team iGEM2005 WiKi documentation.
ECFP Coding Device with Promoter, RBS, Coding Sequence, and Terminator
IPTG Inducible Promoter, RBS, ECFP with LVA tag, Terminator
Test Construct to valid Part I13504 before further assemblies
3OC6HSL+LuxR dependent LacZalpha generator
In the presence of 30C6HSL and LuxR this part will generate LacZ alpha peptide. Requires a lacZ delta m15 strain of e.coli.
This device coupled the promoters PI and PII of rus operon from Acidithiobacillus ferrooxidans with a monomeric red fluorescent protein (BBa_E1010) used as a signal, showing specific sensorbility response to iron ions presence.
Genomic sequence for this part was gotten from NCBI (AJ006456)
Untagged GFP behind a constitutive promoter.
reporter device for I14016. dominantly repressed by lambda cI
Contains IPTG inducible promoter, an RBS, GFP (+ LVA tag), and a terminator.
This part will be made using the standard ligation tecniques.
Prm+ promoter of the lambda-system (with OR3 region deactivated over mutation) with YFP as a reporter. It is needed in subsequent parts to observe the performance of the complete input-system, which is based on the modified lambda-system.
a similar part is available in registry. we designed this as we faced some problems in CFP expression with the earlier part. we have tested this part and it is functional.
This part was constructed from parts available through the registry.
Lambda-system Pr promoter with CFP(no degradation tag) reporter
Testing Pr base level activity with specific reporter CFP without degradation tag for longer lifetime.
Lac operator with CFP reporter (with LVA tag) [R/Lc+]
Christopher Batten, Victoria Chou, Kenneth Nesmith
Stationary phase dependent GFP generator
This part is a composite part consisting of J45992 (a stationary phase promoter) and E0840 (a GFP generator). Thus, this device creates green fluorescent protein in stationary phase.
This part was mainly designed to test whether J45250 would smell like banana only in stationary phase.
J45992 was PCRed out of the E. coli genome, while E0840 was constructed at the Registry of Standard Biological Parts.
Prm+ promoter of the lambda-system (with OR3 region deactivated over mutation) with RFP as a reporter. It is needed in subsequent parts to observe the performance of the complete input-system, which is based on the modified lambda-system.
For more details see Sensing Device and Event Processing Device documentation.
Prm+ promoter of the lambda-system (with OR3 region deactivated over mutation) with a CFP reporter. The purpose is to roughly quantify its relative basal activity compared with the constitutive activity of the Pr promoter.
Promoter activated by OmpR-P with the reporter GFP
Many signalling cellular processes occur using a phosphorylation mechanism. In order to assemble a transmembranal pathway, that frees a phosphore, with a genetic network within the cell, we use the promoter pOmpR (R0082). In E. coli, the osmosensing machinery using the EnvZ membrane protein follows this mechanism. That promoter is regulated negatively (repressed) by the transcription factor OmpR-P. We put a well known protein downstream: GFP (E0040) with a RBS (B0034) and a Terminator (B0015). This composite part will be very useful to characterize many cellular biosensors.
To make this composite part we assemby two parts from the Registry (a promoter (R0082) and a generator (I13504)).
Lactose/IPTG inducible promoter with RBS, RFP reporter tagged with LAA-ssrA degradation tag and terminator. The tagged RFP is actively degraded within cell, thus provides better temporal resolution of red fluorescence and promoter activity.
Part was assembled using BrickClip assembly (BBF RFC 104) using other biobrick parts as templates. BrickClip assembly is a special case of more general Paperclip assembly method.[1]
Lambda-system Pr promoter with YFP(no degradation tag) reporter
Testing Pr base level activity with specific reporter YFP without degradation tag for longer lifetime.
Pr promoter of the lambda-system combined with GFP as a quantification and debugging device for the INPUT-module of the ETHZ05 iGEM team.
GFP coding device composed by: -LacI Promoter switched on by IPTG (ROO10) -RBS with efficiency 1 (B0034) -GFP green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP(E0040) - Excitation max - 501nm Emission max - 511nm -Terminators (B0010, B0012)
Different biobricks are avaible: Plac+RBS, RBS+GFP, RBS+GFP+Term, GFP+Term
Periplasm transport visualization device
Two terminators in Bgl operon that are antiterminated by bglG with YFP reportor
constitutive GFP (AAV+) device, medium RBS
Lactose/IPTG inducible promoter with GFP reporter tagged with AAV-ssrA degradation tag followed by terminator. The tagged GFP is actively degraded within cell, thus provides better temporal resolution of green fluorescence and promoter activity.
Lactose/IPTG inducible promoter with GFP reporter tagged with NYADAS-ssrA degradation tag followed by terminator. The tagged GFP is actively degraded within cell, thus provides better temporal resolution of green fluorescence and promoter activity.
RFP Coding Device - RFC10 and RFC25 compatible
Red Fluorescent Protein from Discosoma striata BBa_E1010
Mutated promoter activated by OmpR-P with the reporter GFP
Many signalling cellular processes occur using a phosphorylation mechanism. In order to assemble a transmembranal pathway, that frees a phosphore, with a genetic network within the cell, we can use this promoter (R0083). That promoter is regulated positively (activated) by the transcription factor OmpR-P, and it contains only one operator in its sequence, whereas in the natural promoter (R0082) there are three. We put a well known protein downstream: GFP (E0040) with a RBS (B0034) and a Terminator (B0015). This composite part will be very useful to characterize many cellular biosensors, jointly with the part J58102.
To make this composite part we assemby two parts from the Registry (a promoter (R0083) and a generator (I13504)).
This is a GFP generator with a hypoxia inducible promoter
The panas promoter is self-designed The rbs, GFP and the terminator are from the registry
This part is a generator for the fusion protein invisine(1-625)-RFP. The pTet promoter is constitutively on and can be repressed by TetR repressor protein.
The fusion protein invisine(1-625)-RFP is self-designed The pTet promoter, rbs and the terminator are from the registry
Lambda-system Pr promoter with YFP reporter
Testing Pr base level activity with specific reporter YFP.
promoter lambda (cI regulated) with RFP reporter
Promoter lambda - cI regulated - (R0051) with RFP (E1010) as a reporter. It can be used as a detector of the glucose metabolism.
Hybrid Lux promoter (repressed by cI) activates YFP reporter.
This part produces GFP in stationary phase via a truncated version of the osmY promoter (J45993).
Lac operator with CFP reporter (without LVA tag) [R/Lc-]
Lactose/IPTG inducible promoter with RBS, RFP reporter tagged with LVA-ssrA degradation tag and terminator. The tagged RFP is actively degraded within cell, thus provides better temporal resolution of red fluorescence and promoter activity. This part can be considered as improved version of BBa_J04451 as it does not contain StuI restriction site coding for additional 'RP' residues between RFP and LVA tag.
GFP Coding Device with promoter, RBS, GFP with LVA tag, and Terminator
IPTG inducible promoter, RBS, GFP with LVA tag, and terminator.
Pr promoter of the lambda-system combined with CFP as a quantification and debugging device for the INPUT-module of the ETHZ05 iGEM team.
Hybrid Las promoter (repressed by cI) activates YFP reporter.
Lactose/IPTG inducible promoter wuth RFP reporter tagged with AAV-ssrA degradation tag followed by terminator. The tagged RFP is actively degraded within cell, thus provides better temporal resolution of red fluorescence and promoter activity. Part was assembled using BrickClip assembly (BBF RFC 104) using other biobrick parts as templates. BrickClip assembly is a special case of more general Paperclip assembly method.[1] Reference [1] Trubitsyna, M., Michlewski, G., Cai, Y., Elfick, A. & French, C. E. PaperClip: rapid multi-part DNA assembly from existing libraries. Nucleic Acids Res. 1???8 (2014). doi:10.1093/nar/gku829
Prm+ promoter of the lambda-system (with OR3 region deactivated over mutation) with a GFP reporter. The purpose is to roughly quantify its relative basal activity compared with the constitutive activity of the Pr promoter.
This is a twin of BBa_J04430. The Registry is using the assembly of this part as a control in robotic assembly. Since it is a GFP reporter we can assemble it and use the results as an indication of whether or not our assembly was successful.
Tetracycline/aTc inducible promoter with GFP reporter tagged with DAS-ssrA degradation tag followed by terminator. The tagged GFP is actively degraded within cell, thus provides better temporal resolution of green fluorescence and promoter activity.Presence of SspB is crucial for effective protein degradation.
Contains an IPTG inducible promoter an RBS, RFP, and terminator.
EYFP, cI activated/cI434 repressed promoter
Designed to test whether our Pulse Generation circuit can give transient gene expression
Construct for testing the empty screening plasmid. This is the downstream part of the SP (psb1A0) after a part is inserted.
Test construct to validate part J06702 before use in future assemblies
Mutagenesis was used to add a Nde1 site at the start of the RFP coding region of the biobrick BBa_J04450. This enables a restriction digest with Nde1 to be performed allowing the RFP coding region to be excised from the plasmid.
Nde1 site was added at the start of the RFP coding region.
a similar part is available in registry. we designed this as we faced some problems in YFP expression with the earlier part. we have tested this part and it is functional.
Positively regulated, OmpR-controlled promoter from OmpC gene (R0082) directing transcription of lacZ reporter device (I2012).
Test construct for Screening Plasmid insertion site (No Promoter)
Tet operator with CFP reporter (with LVA tag) [R/Tc+]
Promoter activated synergistically by cI and CRP with the reporter RFP
A promoter activated synergistically by cI and CRP proteins (part ref. BBa_J58011) with a generator of the reporter RFP (part ref. BBa_I13507). This generator uses the following parts: BBa_B0034 (RBS), BBa_E1010 (mRFP1), and BBa_B0015 (Terminator). With this construction, we obtain an AND gate, with RFP as output, and cI and CRP as inputs.
Notice that in the promoter sequence the XbaI restricction site is present. Therefore, we have to digest this part upstream only with EcoRI.
To make this composite part we assemby two parts from the Registry (a promoter and a generator).
In the presence of LacI, the pLac promoter will be tightly repressed and not produce mRFP
Lactose/IPTG inducible promoter with GFP reporter tagged with DAS-ssrA degradation tag followed by terminator. The tagged GFP is actively degraded within cell, thus provides better temporal resolution of green fluorescence and promoter activity. Presence of SspB is crucial for effective protein degradation.
Test construct for FecA Promoter. Includes FecA Promoter (J07019) and RFG (I13507).
LuxR controlled operator in front of POPS-->RFP part.
GFP expression system that uses dedicated translation
This expression device requires the presence of dedicated ribosomes to produce GFP in E. coli. It will be tested in conjuction with E70101 which requires both dedicated ribosomes and dedicated transcription.
This part is a generator for RFP under the prosseA promoter
The prosseA promoter is self-designed The rbs, RFP and the terminator are from the registry
Using the orthogonal system to regulate protein expression
GFP expression device controlled by an arabinose inducible pBAD promoter
This part synthesizes GFP using a common RBS and terminator. The promoter is inducible by arabinose. This small molecule binds to the ara repressor (contained within I0500), drepressing the reporter.
Standard GFP expression device with sRBS
Tetracycline/aTc inducible promoter with GFP reporter tagged with NYADAS-ssrA degradation tag followed by terminator. The tagged GFP is actively degraded within cell, thus provides better temporal resolution of green fluorescence and promoter activity.
Lactose/IPTG inducible promoter with RFP reporter tagged with DAS-ssrA degradation tag followed by terminator. The tagged RFP is degraded within cell, thus provides better temporal resolution of red fluorescence and promoter activity. Presence of SspB is crucial for effective protein degradation.
Arabinose will induce the pBad promoter and produce mRFP
Tetracycline/aTc inducible promoter with GFP reporter tagged with LVA-ssrA degradation tag followed by terminator. The tagged GFP is actively degraded within cell, thus provides better temporal resolution of green fluorescence and promoter activity.
This part is a reporter construct designed to let us know the activity of the ToxR regulated promoter, ctx.
This part contains an IPTG inducible promoter, RBS, RFP, terminator, and LVA tag.
Tetracycline/aTc inducible promoter with GFP reporter tagged with AAV-ssrA degradation tag followed by terminator. The tagged GFP is actively degraded within cell, thus provides better temporal resolution of green fluorescence and promoter activity.