//classic/reporter/pret
type: Collection
persistent identity: classic reporter pret
display id: classic_reporter_pret
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
owned by: james
owned by: myers
member: BBa_E70102
member: BBa_I2031
member: BBa_K1399016
member: BBa_J32029
member: BBa_I13521
member: BBa_I741100
member: BBa_J5523
member: Pr+YFP-
member: BBa_J21001
member: BBa_K283015
member: BBa_J09266
member: p(osmY).GF
member: Pr+RFP
member: BBa_J22203
member: BBa_K1399019
member: BBa_J47862
member: BBa_K1399013
member: Prm+GFP
member: BBa_J04431
member: BBa_I741104
member: BBa_J58103
member: Pr + GFP
member: pLambda+RF
member: mRFP
member: BBa_K1399012
member: Little Bub
member: BBa_I13580
member: BBa_J58102
member: BBa_J3901
member: BBa_I13571
member: pLac+GFP
member: BBa_J06005
member: BBa_J5527
member: BBa_J06103
member: Prm+ YFP
member: BBa_E70103
member: BBa_J07066
member: Pr+CFP-
member: BBa_I13522
member: BBa_I13578
member: BBa_J47863
member: BBa_J13003
member: BBa_K1399022
member: BBa_J13020
member: BBa_I13601
member: BBa_K1067008
member: BBa_E70101
member: BBa_K1067001
member: BBa_I723124
member: BBa_K1399020
member: BBa_J07034
member: BBa_K1041000
member: Pr+CFP
member: BBa_J69501
member: BBa_K1399011
member: GFP
member: BBa_I13603
member: BBa_J58101
member: BBa_I741102
member: BBa_J22202
member: BBa_K1399023
member: BBa_E70202
member: BBa_J04450
member: BBa_J07011
member: BBa_K1399014
member: BBa_J13025
member: Prm+RFP
member: BBa_I13600
member: BBa_K1399018
member: Pulsegen t
member: BBa_J06003
member: BBa_C0470

GFP
type: ComponentDefinition
persistent identity: BBa J5528
display id: BBa_J5528
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Arabinose -> pBad = GFP
creator: Charles Yoon
created: 2006-10-30T12:00:00Z
modified: 2015-08-31T03:22:59Z
discontinued: false
dominant: false
experience: experience/None
group u list: _82_
m user id: 0
owner id: 840
owning group id: 82
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Similar to part J5527 where mRFP is replaced with GFP
mutable notes: none
mutable provenance: iGEM 2006 Registry
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_I0500
component: BBa_E0040
component: BBa_B0010
component: BBa_B0012
component: BBa_B0034
sequence annotation: BBa_B0034
sequence annotation: BBa_B0012
sequence annotation: BBa_B0010
sequence annotation: BBa_E0040
sequence annotation: BBa_I0500
sequence: https://synbiohub.org/public/igem/BBa_J5528_sequence/1

BBa_I0500
type: Component
persistent identity: component1908656
display id: component1908656
version: 1
top level: GFP
access: public
definition: https://synbiohub.org/public/igem/BBa_I0500/1

BBa_E0040
type: Component
persistent identity: component1908659
display id: component1908659
version: 1
top level: GFP
access: public
definition: https://synbiohub.org/public/igem/BBa_E0040/1

BBa_B0010
type: Component
persistent identity: component1908660
display id: component1908660
version: 1
top level: GFP
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
type: Component
persistent identity: component1908662
display id: component1908662
version: 1
top level: GFP
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0034
type: Component
persistent identity: component1908658
display id: component1908658
version: 1
top level: GFP
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1908658
display id: annotation1908658
version: 1
top level: GFP
location: https://synbiohub.org/public/igem/BBa_J5528/annotation1908658/range1908658/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_J5528/annotation1908658/range1908658/1
type: Range
persistent identity: range1908658
display id: range1908658
version: 1
direction: direction/reverse
top level: GFP
start: 1219
end: 1230
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1908662
display id: annotation1908662
version: 1
top level: GFP
location: https://synbiohub.org/public/igem/BBa_J5528/annotation1908662/range1908662/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_J5528/annotation1908662/range1908662/1
type: Range
persistent identity: range1908662
display id: range1908662
version: 1
direction: direction/forward
top level: GFP
start: 2053
end: 2093
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1908660
display id: annotation1908660
version: 1
top level: GFP
location: https://synbiohub.org/public/igem/BBa_J5528/annotation1908660/range1908660/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_J5528/annotation1908660/range1908660/1
type: Range
persistent identity: range1908660
display id: range1908660
version: 1
direction: direction/forward
top level: GFP
start: 1965
end: 2044
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation1908659
display id: annotation1908659
version: 1
top level: GFP
location: https://synbiohub.org/public/igem/BBa_J5528/annotation1908659/range1908659/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_J5528/annotation1908659/range1908659/1
type: Range
persistent identity: range1908659
display id: range1908659
version: 1
direction: direction/reverse
top level: GFP
start: 1237
end: 1956
orientation: inline

BBa_I0500
type: SequenceAnnotation
persistent identity: annotation1908656
display id: annotation1908656
version: 1
top level: GFP
location: https://synbiohub.org/public/igem/BBa_J5528/annotation1908656/range1908656/1
component: BBa_I0500

https://synbiohub.org/public/igem/BBa_J5528/annotation1908656/range1908656/1
type: Range
persistent identity: range1908656
display id: range1908656
version: 1
direction: direction/reverse
top level: GFP
start: 1
end: 1210
orientation: inline

BBa_K1399021
type: ComponentDefinition
persistent identity: BBa K1399021
display id: BBa_K1399021
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ptet-GFP(LAA)
creator: Anna Stikane
created: 2014-09-18T11:00:00Z
modified: 2015-05-08T01:10:16Z
discontinued: false
dominant: false
group u list: _1777_
m user id: 0
owner id: 22477
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Tetracycline/aTc inducible promoter with GFP reporter tagged with LAA-ssrA degradation tag followed by terminator. The tagged GFP is actively degraded within cell, thus provides better temporal resolution of green fluorescence and promoter activity.
mutable notes: Part was assembled using BrickClip assembly (BBF RFC104) using other biobrick parts as templates. BrickClip assembly is a special case of more general Paperclip assembly method.[1]
mutable provenance: other parts
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_K1399006
component: BBa_R0040
component: BBa_B0015
component: BBa_B0034
sequence annotation: BBa_K1399006
sequence annotation: BBa_R0040
sequence annotation: BBa_B0015
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K1399021_sequence/1

BBa_K1399006
type: Component
persistent identity: component2384271
display id: component2384271
version: 1
top level: BBa_K1399021
access: public
definition: https://synbiohub.org/public/igem/BBa_K1399006/1

BBa_R0040
type: Component
persistent identity: component2384259
display id: component2384259
version: 1
top level: BBa_K1399021
access: public
definition: https://synbiohub.org/public/igem/BBa_R0040/1

BBa_B0015
type: Component
persistent identity: component2384278
display id: component2384278
version: 1
top level: BBa_K1399021
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_B0034
type: Component
persistent identity: component2384265
display id: component2384265
version: 1
top level: BBa_K1399021
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K1399006
type: SequenceAnnotation
persistent identity: annotation2384271
display id: annotation2384271
version: 1
top level: BBa_K1399021
location: https://synbiohub.org/public/igem/BBa_K1399021/annotation2384271/range2384271/1
component: BBa_K1399006

https://synbiohub.org/public/igem/BBa_K1399021/annotation2384271/range2384271/1
type: Range
persistent identity: range2384271
display id: range2384271
version: 1
direction: direction/reverse
top level: BBa_K1399021
start: 81
end: 833
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2384259
display id: annotation2384259
version: 1
top level: BBa_K1399021
location: https://synbiohub.org/public/igem/BBa_K1399021/annotation2384259/range2384259/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_K1399021/annotation2384259/range2384259/1
type: Range
persistent identity: range2384259
display id: range2384259
version: 1
direction: direction/forward
top level: BBa_K1399021
start: 1
end: 54
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2384278
display id: annotation2384278
version: 1
top level: BBa_K1399021
location: https://synbiohub.org/public/igem/BBa_K1399021/annotation2384278/range2384278/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K1399021/annotation2384278/range2384278/1
type: Range
persistent identity: range2384278
display id: range2384278
version: 1
direction: direction/reverse
top level: BBa_K1399021
start: 842
end: 970
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2384265
display id: annotation2384265
version: 1
top level: BBa_K1399021
location: https://synbiohub.org/public/igem/BBa_K1399021/annotation2384265/range2384265/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1399021/annotation2384265/range2384265/1
type: Range
persistent identity: range2384265
display id: range2384265
version: 1
direction: direction/reverse
top level: BBa_K1399021
start: 63
end: 74
orientation: inline

BBa_J13003
type: ComponentDefinition
persistent identity: BBa J13003
display id: BBa_J13003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: POPS+RIPS generates CFP. POPS generates YFP
creator: Jeff Tabor
created: 2005-06-15T11:00:00Z
modified: 2015-08-31T04:08:29Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _37_5_
m user id: 0
owner id: 88
owning group id: 37
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_B0010
component: BBa_B0012
component: BBa_E0020
component: BBa_E0030
component: BBa_B0034
sequence annotation: BBa_B0012
sequence annotation: BBa_B0034
sequence annotation: BBa_E0030
sequence annotation: BBa_B0010
sequence annotation: BBa_E0020
sequence: https://synbiohub.org/public/igem/BBa_J13003_sequence/1

BBa_B0010
type: Component
persistent identity: component1523622
display id: component1523622
version: 1
top level: BBa_J13003
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
type: Component
persistent identity: component1523632
display id: component1523632
version: 1
top level: BBa_J13003
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_E0020
type: Component
persistent identity: component1523610
display id: component1523610
version: 1
top level: BBa_J13003
access: public
definition: https://synbiohub.org/public/igem/BBa_E0020/1

BBa_E0030
type: Component
persistent identity: component1523617
display id: component1523617
version: 1
top level: BBa_J13003
access: public
definition: https://synbiohub.org/public/igem/BBa_E0030/1

BBa_B0034
type: Component
persistent identity: component1523615
display id: component1523615
version: 1
top level: BBa_J13003
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1523632
display id: annotation1523632
version: 1
top level: BBa_J13003
location: https://synbiohub.org/public/igem/BBa_J13003/annotation1523632/range1523632/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_J13003/annotation1523632/range1523632/1
type: Range
persistent identity: range1523632
display id: range1523632
version: 1
direction: direction/forward
top level: BBa_J13003
start: 1569
end: 1609
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1523615
display id: annotation1523615
version: 1
top level: BBa_J13003
location: https://synbiohub.org/public/igem/BBa_J13003/annotation1523615/range1523615/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_J13003/annotation1523615/range1523615/1
type: Range
persistent identity: range1523615
display id: range1523615
version: 1
direction: direction/forward
top level: BBa_J13003
start: 732
end: 743
orientation: inline

BBa_E0030
type: SequenceAnnotation
persistent identity: annotation1523617
display id: annotation1523617
version: 1
top level: BBa_J13003
location: https://synbiohub.org/public/igem/BBa_J13003/annotation1523617/range1523617/1
component: BBa_E0030

https://synbiohub.org/public/igem/BBa_J13003/annotation1523617/range1523617/1
type: Range
persistent identity: range1523617
display id: range1523617
version: 1
direction: direction/forward
top level: BBa_J13003
start: 750
end: 1472
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1523622
display id: annotation1523622
version: 1
top level: BBa_J13003
location: https://synbiohub.org/public/igem/BBa_J13003/annotation1523622/range1523622/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_J13003/annotation1523622/range1523622/1
type: Range
persistent identity: range1523622
display id: range1523622
version: 1
direction: direction/forward
top level: BBa_J13003
start: 1481
end: 1560
orientation: inline

BBa_E0020
type: SequenceAnnotation
persistent identity: annotation1523610
display id: annotation1523610
version: 1
top level: BBa_J13003
location: https://synbiohub.org/public/igem/BBa_J13003/annotation1523610/range1523610/1
component: BBa_E0020

https://synbiohub.org/public/igem/BBa_J13003/annotation1523610/range1523610/1
type: Range
persistent identity: range1523610
display id: range1523610
version: 1
direction: direction/forward
top level: BBa_J13003
start: 1
end: 723
orientation: inline

Pr+RFP
type: ComponentDefinition
persistent identity: BBa S03473
display id: BBa_S03473
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Lambda-system Pr promoter with RFP reporter
creator: ETH Zurich iGEM Team 2005
created: 2006-05-01T11:00:00Z
modified: 2015-05-08T01:14:23Z
discontinued: false
dominant: true
group u list: _34_
m user id: 0
owner id: 398
owning group id: 34
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Testing Pr base level activity with specific reporter RFP.
mutable notes: For more details see Sensing Device and Event Processing Device in the ETH Team iGEM2005 WiKi documentation.
mutable provenance: Registry 7.05
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_I13507
component: BBa_R0051
sequence annotation: BBa_I13507
sequence annotation: BBa_R0051
sequence: https://synbiohub.org/public/igem/BBa_S03473_sequence/1

BBa_I13507
type: Component
persistent identity: component2251458
display id: component2251458
version: 1
top level: Pr+RFP
access: public
definition: https://synbiohub.org/public/igem/BBa_I13507/1

BBa_R0051
type: Component
persistent identity: component2251443
display id: component2251443
version: 1
top level: Pr+RFP
access: public
definition: https://synbiohub.org/public/igem/BBa_R0051/1

BBa_I13507
type: SequenceAnnotation
persistent identity: annotation2251458
display id: annotation2251458
version: 1
top level: Pr+RFP
location: https://synbiohub.org/public/igem/BBa_S03473/annotation2251458/range2251458/1
component: BBa_I13507

https://synbiohub.org/public/igem/BBa_S03473/annotation2251458/range2251458/1
type: Range
persistent identity: range2251458
display id: range2251458
version: 1
direction: direction/reverse
top level: Pr+RFP
start: 58
end: 918
orientation: inline

BBa_R0051
type: SequenceAnnotation
persistent identity: annotation2251443
display id: annotation2251443
version: 1
top level: Pr+RFP
location: https://synbiohub.org/public/igem/BBa_S03473/annotation2251443/range2251443/1
component: BBa_R0051

https://synbiohub.org/public/igem/BBa_S03473/annotation2251443/range2251443/1
type: Range
persistent identity: range2251443
display id: range2251443
version: 1
direction: direction/forward
top level: Pr+RFP
start: 1
end: 49
orientation: inline

BBa_J04421
type: ComponentDefinition
persistent identity: BBa J04421
display id: BBa_J04421
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: ECFP Coding Device with Promoter, RBS, Coding Sequence, and Terminator
creator: Kristen DeCelle
created: 2005-06-08T11:00:00Z
modified: 2016-10-14T07:25:32Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _16_
m user id: 31248
owner id: 326
owning group id: 16
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: IPTG Inducible Promoter, RBS, ECFP with LVA tag, Terminator
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_B0012
component: BBa_B0010
component: BBa_R0010
component: BBa_B0034
component: BBa_E0022
sequence annotation: BBa_E0022
sequence annotation: BBa_B0034
sequence annotation: BBa_B0012
sequence annotation: BBa_B0010
sequence annotation: BBa_R0010
sequence: https://synbiohub.org/public/igem/BBa_J04421_sequence/1

BBa_B0012
type: Component
persistent identity: component1509538
display id: component1509538
version: 1
top level: BBa_J04421
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0010
type: Component
persistent identity: component1509528
display id: component1509528
version: 1
top level: BBa_J04421
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_R0010
type: Component
persistent identity: component1509498
display id: component1509498
version: 1
top level: BBa_J04421
access: public
definition: https://synbiohub.org/public/igem/BBa_R0010/1

BBa_B0034
type: Component
persistent identity: component1509508
display id: component1509508
version: 1
top level: BBa_J04421
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_E0022
type: Component
persistent identity: component1509520
display id: component1509520
version: 1
top level: BBa_J04421
access: public
definition: https://synbiohub.org/public/igem/BBa_E0022/1

BBa_E0022
type: SequenceAnnotation
persistent identity: annotation1509520
display id: annotation1509520
version: 1
top level: BBa_J04421
location: https://synbiohub.org/public/igem/BBa_J04421/annotation1509520/range1509520/1
component: BBa_E0022

https://synbiohub.org/public/igem/BBa_J04421/annotation1509520/range1509520/1
type: Range
persistent identity: range1509520
display id: range1509520
version: 1
direction: direction/forward
top level: BBa_J04421
start: 227
end: 988
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1509508
display id: annotation1509508
version: 1
top level: BBa_J04421
location: https://synbiohub.org/public/igem/BBa_J04421/annotation1509508/range1509508/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_J04421/annotation1509508/range1509508/1
type: Range
persistent identity: range1509508
display id: range1509508
version: 1
direction: direction/forward
top level: BBa_J04421
start: 209
end: 220
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1509538
display id: annotation1509538
version: 1
top level: BBa_J04421
location: https://synbiohub.org/public/igem/BBa_J04421/annotation1509538/range1509538/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_J04421/annotation1509538/range1509538/1
type: Range
persistent identity: range1509538
display id: range1509538
version: 1
direction: direction/forward
top level: BBa_J04421
start: 1085
end: 1125
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1509528
display id: annotation1509528
version: 1
top level: BBa_J04421
location: https://synbiohub.org/public/igem/BBa_J04421/annotation1509528/range1509528/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_J04421/annotation1509528/range1509528/1
type: Range
persistent identity: range1509528
display id: range1509528
version: 1
direction: direction/forward
top level: BBa_J04421
start: 997
end: 1076
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation1509498
display id: annotation1509498
version: 1
top level: BBa_J04421
location: https://synbiohub.org/public/igem/BBa_J04421/annotation1509498/range1509498/1
component: BBa_R0010

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type: Range
persistent identity: range1509498
display id: range1509498
version: 1
direction: direction/forward
top level: BBa_J04421
start: 1
end: 200
orientation: inline

BBa_J69501
type: ComponentDefinition
persistent identity: BBa J69501
display id: BBa_J69501
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CI-repressible GFP Reporter
creator: James Brown
created: 2007-03-28T11:00:00Z
modified: 2015-05-08T01:08:18Z
discontinued: false
dominant: false
group u list: _101_
m user id: 0
owner id: 740
owning group id: 101
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Test Construct to valid Part I13504 before further assemblies
mutable notes: NA
mutable provenance: Registry parts
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_B0034
component: BBa_E0040
component: BBa_B0012
component: BBa_B0010
component: BBa_R0051
sequence annotation: BBa_B0010
sequence annotation: BBa_R0051
sequence annotation: BBa_B0034
sequence annotation: BBa_E0040
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_J69501_sequence/1

BBa_B0034
type: Component
persistent identity: component1923072
display id: component1923072
version: 1
top level: BBa_J69501
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_E0040
type: Component
persistent identity: component1923073
display id: component1923073
version: 1
top level: BBa_J69501
access: public
definition: https://synbiohub.org/public/igem/BBa_E0040/1

BBa_B0012
type: Component
persistent identity: component1923076
display id: component1923076
version: 1
top level: BBa_J69501
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0010
type: Component
persistent identity: component1923074
display id: component1923074
version: 1
top level: BBa_J69501
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_R0051
type: Component
persistent identity: component1923067
display id: component1923067
version: 1
top level: BBa_J69501
access: public
definition: https://synbiohub.org/public/igem/BBa_R0051/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1923074
display id: annotation1923074
version: 1
top level: BBa_J69501
location: https://synbiohub.org/public/igem/BBa_J69501/annotation1923074/range1923074/1
component: BBa_B0010

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type: Range
persistent identity: range1923074
display id: range1923074
version: 1
direction: direction/forward
top level: BBa_J69501
start: 804
end: 883
orientation: inline

BBa_R0051
type: SequenceAnnotation
persistent identity: annotation1923067
display id: annotation1923067
version: 1
top level: BBa_J69501
location: https://synbiohub.org/public/igem/BBa_J69501/annotation1923067/range1923067/1
component: BBa_R0051

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type: Range
persistent identity: range1923067
display id: range1923067
version: 1
direction: direction/forward
top level: BBa_J69501
start: 1
end: 49
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1923072
display id: annotation1923072
version: 1
top level: BBa_J69501
location: https://synbiohub.org/public/igem/BBa_J69501/annotation1923072/range1923072/1
component: BBa_B0034

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type: Range
persistent identity: range1923072
display id: range1923072
version: 1
direction: direction/reverse
top level: BBa_J69501
start: 58
end: 69
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation1923073
display id: annotation1923073
version: 1
top level: BBa_J69501
location: https://synbiohub.org/public/igem/BBa_J69501/annotation1923073/range1923073/1
component: BBa_E0040

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type: Range
persistent identity: range1923073
display id: range1923073
version: 1
direction: direction/reverse
top level: BBa_J69501
start: 76
end: 795
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1923076
display id: annotation1923076
version: 1
top level: BBa_J69501
location: https://synbiohub.org/public/igem/BBa_J69501/annotation1923076/range1923076/1
component: BBa_B0012

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type: Range
persistent identity: range1923076
display id: range1923076
version: 1
direction: direction/forward
top level: BBa_J69501
start: 892
end: 932
orientation: inline

BBa_J13000
type: ComponentDefinition
persistent identity: BBa J13000
display id: BBa_J13000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: 3OC6HSL+LuxR dependent LacZalpha generator
creator: Laura Lavery
created: 2005-05-22T11:00:00Z
modified: 2015-08-31T04:08:29Z
discontinued: false
dominant: true
experience: experience/Issues
group u list: _37_
m user id: 0
owner id: 327
owning group id: 37
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: In the presence of 30C6HSL and LuxR this part will generate LacZ alpha peptide. Requires a lacZ delta m15 strain of e.coli.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_R0062
component: BBa_E0433
sequence annotation: BBa_E0433
sequence annotation: BBa_R0062
sequence: https://synbiohub.org/public/igem/BBa_J13000_sequence/1

BBa_R0062
type: Component
persistent identity: component2221668
display id: component2221668
version: 1
top level: BBa_J13000
access: public
definition: https://synbiohub.org/public/igem/BBa_R0062/1

BBa_E0433
type: Component
persistent identity: component2221693
display id: component2221693
version: 1
top level: BBa_J13000
access: public
definition: https://synbiohub.org/public/igem/BBa_E0433/1

BBa_E0433
type: SequenceAnnotation
persistent identity: annotation2221693
display id: annotation2221693
version: 1
top level: BBa_J13000
location: https://synbiohub.org/public/igem/BBa_J13000/annotation2221693/range2221693/1
component: BBa_E0433

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type: Range
persistent identity: range2221693
display id: range2221693
version: 1
direction: direction/reverse
top level: BBa_J13000
start: 64
end: 571
orientation: inline

BBa_R0062
type: SequenceAnnotation
persistent identity: annotation2221668
display id: annotation2221668
version: 1
top level: BBa_J13000
location: https://synbiohub.org/public/igem/BBa_J13000/annotation2221668/range2221668/1
component: BBa_R0062

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type: Range
persistent identity: range2221668
display id: range2221668
version: 1
direction: direction/forward
top level: BBa_J13000
start: 1
end: 55
orientation: inline

BBa_J3901
type: ComponentDefinition
persistent identity: BBa J3901
display id: BBa_J3901
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PrFe-mRFP1
creator: Latin American iGEM team
created: 2006-10-28T11:00:00Z
modified: 2015-08-31T04:08:48Z
discontinued: false
dominant: false
experience: experience/Fails
group u list: _67_
m user id: 0
owner id: 1000
owning group id: 67
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This device coupled the promoters PI and PII of rus operon from Acidithiobacillus ferrooxidans with a monomeric red fluorescent protein (BBa_E1010) used as a signal, showing specific sensorbility response to iron ions presence.
mutable notes: None
mutable provenance: Genomic sequence for this part was gotten from NCBI (AJ006456)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_E1010
component: BBa_B0034
component: BBa_B0015
component: BBa_J3902
component: BBa_R0010
sequence annotation: BBa_B0034
sequence annotation: BBa_E1010
sequence annotation: BBa_R0010
sequence annotation: BBa_J3902
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_J3901_sequence/1

BBa_E1010
type: Component
persistent identity: component2260464
display id: component2260464
version: 1
top level: BBa_J3901
access: public
definition: https://synbiohub.org/public/igem/BBa_E1010/1

BBa_B0034
type: Component
persistent identity: component2260461
display id: component2260461
version: 1
top level: BBa_J3901
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0015
type: Component
persistent identity: component2260471
display id: component2260471
version: 1
top level: BBa_J3901
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_J3902
type: Component
persistent identity: component2260452
display id: component2260452
version: 1
top level: BBa_J3901
access: public
definition: https://synbiohub.org/public/igem/BBa_J3902/1

BBa_R0010
type: Component
persistent identity: component2260453
display id: component2260453
version: 1
top level: BBa_J3901
access: public
definition: https://synbiohub.org/public/igem/BBa_R0010/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2260461
display id: annotation2260461
version: 1
top level: BBa_J3901
location: https://synbiohub.org/public/igem/BBa_J3901/annotation2260461/range2260461/1
component: BBa_B0034

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type: Range
persistent identity: range2260461
display id: range2260461
version: 1
direction: direction/reverse
top level: BBa_J3901
start: 489
end: 500
orientation: inline

BBa_E1010
type: SequenceAnnotation
persistent identity: annotation2260464
display id: annotation2260464
version: 1
top level: BBa_J3901
location: https://synbiohub.org/public/igem/BBa_J3901/annotation2260464/range2260464/1
component: BBa_E1010

https://synbiohub.org/public/igem/BBa_J3901/annotation2260464/range2260464/1
type: Range
persistent identity: range2260464
display id: range2260464
version: 1
direction: direction/reverse
top level: BBa_J3901
start: 507
end: 1212
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation2260453
display id: annotation2260453
version: 1
top level: BBa_J3901
location: https://synbiohub.org/public/igem/BBa_J3901/annotation2260453/range2260453/1
component: BBa_R0010

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type: Range
persistent identity: range2260453
display id: range2260453
version: 1
direction: direction/forward
top level: BBa_J3901
start: 281
end: 480
orientation: inline

BBa_J3902
type: SequenceAnnotation
persistent identity: annotation2260452
display id: annotation2260452
version: 1
top level: BBa_J3901
location: https://synbiohub.org/public/igem/BBa_J3901/annotation2260452/range2260452/1
component: BBa_J3902

https://synbiohub.org/public/igem/BBa_J3901/annotation2260452/range2260452/1
type: Range
persistent identity: range2260452
display id: range2260452
version: 1
direction: direction/reverse
top level: BBa_J3901
start: 1
end: 272
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2260471
display id: annotation2260471
version: 1
top level: BBa_J3901
location: https://synbiohub.org/public/igem/BBa_J3901/annotation2260471/range2260471/1
component: BBa_B0015

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type: Range
persistent identity: range2260471
display id: range2260471
version: 1
direction: direction/reverse
top level: BBa_J3901
start: 1221
end: 1349
orientation: inline

BBa_I13522
type: ComponentDefinition
persistent identity: BBa I13522
display id: BBa_I13522
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pTet GFP
creator: jkm
created: 2005-06-30T11:00:00Z
modified: 2015-08-31T04:07:35Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _11_
m user id: 0
owner id: 253
owning group id: 6
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Untagged GFP behind a constitutive promoter.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_B0010
component: BBa_B0034
component: BBa_B0012
component: BBa_R0040
component: BBa_E0040
sequence annotation: BBa_R0040
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_B0034
sequence annotation: BBa_E0040
sequence: https://synbiohub.org/public/igem/BBa_I13522_sequence/1

BBa_B0010
type: Component
persistent identity: component1559471
display id: component1559471
version: 1
top level: BBa_I13522
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0034
type: Component
persistent identity: component1559464
display id: component1559464
version: 1
top level: BBa_I13522
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0012
type: Component
persistent identity: component1559481
display id: component1559481
version: 1
top level: BBa_I13522
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_R0040
type: Component
persistent identity: component1559456
display id: component1559456
version: 1
top level: BBa_I13522
access: public
definition: https://synbiohub.org/public/igem/BBa_R0040/1

BBa_E0040
type: Component
persistent identity: component1559466
display id: component1559466
version: 1
top level: BBa_I13522
access: public
definition: https://synbiohub.org/public/igem/BBa_E0040/1

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1559456
display id: annotation1559456
version: 1
top level: BBa_I13522
location: https://synbiohub.org/public/igem/BBa_I13522/annotation1559456/range1559456/1
component: BBa_R0040

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type: Range
persistent identity: range1559456
display id: range1559456
version: 1
direction: direction/forward
top level: BBa_I13522
start: 1
end: 54
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1559471
display id: annotation1559471
version: 1
top level: BBa_I13522
location: https://synbiohub.org/public/igem/BBa_I13522/annotation1559471/range1559471/1
component: BBa_B0010

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type: Range
persistent identity: range1559471
display id: range1559471
version: 1
direction: direction/forward
top level: BBa_I13522
start: 809
end: 888
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1559481
display id: annotation1559481
version: 1
top level: BBa_I13522
location: https://synbiohub.org/public/igem/BBa_I13522/annotation1559481/range1559481/1
component: BBa_B0012

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type: Range
persistent identity: range1559481
display id: range1559481
version: 1
direction: direction/forward
top level: BBa_I13522
start: 897
end: 937
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1559464
display id: annotation1559464
version: 1
top level: BBa_I13522
location: https://synbiohub.org/public/igem/BBa_I13522/annotation1559464/range1559464/1
component: BBa_B0034

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type: Range
persistent identity: range1559464
display id: range1559464
version: 1
direction: direction/forward
top level: BBa_I13522
start: 63
end: 74
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation1559466
display id: annotation1559466
version: 1
top level: BBa_I13522
location: https://synbiohub.org/public/igem/BBa_I13522/annotation1559466/range1559466/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_I13522/annotation1559466/range1559466/1
type: Range
persistent identity: range1559466
display id: range1559466
version: 1
direction: direction/forward
top level: BBa_I13522
start: 81
end: 800
orientation: inline

BBa_I13571
type: ComponentDefinition
persistent identity: BBa I13571
display id: BBa_I13571
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: 5' Hairpin RFP w Terminator
creator: Jason Kelly
created: 2007-06-07T11:00:00Z
modified: 2015-08-31T04:07:35Z
discontinued: false
dominant: false
group u list: _11_84_
m user id: 0
owner id: 2
owning group id: 84
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: 5' Hairpin RFP w Terminator
mutable notes: .
mutable provenance: .
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_B0015
component: BBa_E1010
component: BBa_I13577
component: BBa_B0034
sequence annotation: BBa_B0034
sequence annotation: BBa_E1010
sequence annotation: BBa_I13577
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_I13571_sequence/1

BBa_B0015
type: Component
persistent identity: component2252711
display id: component2252711
version: 1
top level: BBa_I13571
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_E1010
type: Component
persistent identity: component2252704
display id: component2252704
version: 1
top level: BBa_I13571
access: public
definition: https://synbiohub.org/public/igem/BBa_E1010/1

BBa_I13577
type: Component
persistent identity: component2252699
display id: component2252699
version: 1
top level: BBa_I13571
access: public
definition: https://synbiohub.org/public/igem/BBa_I13577/1

BBa_B0034
type: Component
persistent identity: component2252701
display id: component2252701
version: 1
top level: BBa_I13571
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2252701
display id: annotation2252701
version: 1
top level: BBa_I13571
location: https://synbiohub.org/public/igem/BBa_I13571/annotation2252701/range2252701/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I13571/annotation2252701/range2252701/1
type: Range
persistent identity: range2252701
display id: range2252701
version: 1
direction: direction/reverse
top level: BBa_I13571
start: 44
end: 55
orientation: inline

BBa_E1010
type: SequenceAnnotation
persistent identity: annotation2252704
display id: annotation2252704
version: 1
top level: BBa_I13571
location: https://synbiohub.org/public/igem/BBa_I13571/annotation2252704/range2252704/1
component: BBa_E1010

https://synbiohub.org/public/igem/BBa_I13571/annotation2252704/range2252704/1
type: Range
persistent identity: range2252704
display id: range2252704
version: 1
direction: direction/reverse
top level: BBa_I13571
start: 62
end: 767
orientation: inline

BBa_I13577
type: SequenceAnnotation
persistent identity: annotation2252699
display id: annotation2252699
version: 1
top level: BBa_I13571
location: https://synbiohub.org/public/igem/BBa_I13571/annotation2252699/range2252699/1
component: BBa_I13577

https://synbiohub.org/public/igem/BBa_I13571/annotation2252699/range2252699/1
type: Range
persistent identity: range2252699
display id: range2252699
version: 1
direction: direction/reverse
top level: BBa_I13571
start: 1
end: 35
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2252711
display id: annotation2252711
version: 1
top level: BBa_I13571
location: https://synbiohub.org/public/igem/BBa_I13571/annotation2252711/range2252711/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_I13571/annotation2252711/range2252711/1
type: Range
persistent identity: range2252711
display id: range2252711
version: 1
direction: direction/reverse
top level: BBa_I13571
start: 776
end: 904
orientation: inline

BBa_E70103
type: ComponentDefinition
persistent identity: BBa E70103
display id: BBa_E70103
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Reporter for dedicated translation
creator: Barry Canton
created: 2006-03-03T12:00:00Z
modified: 2015-08-31T04:07:27Z
discontinued: false
dominant: false
group u list: _11_6_
m user id: 0
owner id: 135
owning group id: 6
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_B0012
component: BBa_R0040
component: BBa_B0071
component: BBa_B0010
component: BBa_E0040
sequence annotation: BBa_B0012
sequence annotation: BBa_R0040
sequence annotation: BBa_E0040
sequence annotation: BBa_B0071
sequence annotation: BBa_B0010
sequence: https://synbiohub.org/public/igem/BBa_E70103_sequence/1

BBa_B0012
type: Component
persistent identity: component1815622
display id: component1815622
version: 1
top level: BBa_E70103
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_R0040
type: Component
persistent identity: component1815600
display id: component1815600
version: 1
top level: BBa_E70103
access: public
definition: https://synbiohub.org/public/igem/BBa_R0040/1

BBa_B0071
type: Component
persistent identity: component1815605
display id: component1815605
version: 1
top level: BBa_E70103
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BBa_E0040
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BBa_I741101
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display id: BBa_I741101
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter (I741017) and GFP (E0240)
creator: Lucien Weiss
created: 2007-06-20T11:00:00Z
modified: 2015-08-31T04:08:02Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _143_
m user id: 0
owner id: 1763
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: generates GFP
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_B0015
component: BBa_B0030
component: BBa_E0040
component: BBa_I741017
sequence annotation: BBa_E0040
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sequence annotation: BBa_B0030
sequence annotation: BBa_I741017
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BBa_B0015
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BBa_B0030
type: Component
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BBa_E0040
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BBa_I741017
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BBa_E0040
type: SequenceAnnotation
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BBa_B0015
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BBa_B0030
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BBa_I741017
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BBa_J13020
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display id: BBa_J13020
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: AI-1+LasR dependent EYFP generator
creator: Jeff Tabor
created: 2005-06-28T11:00:00Z
modified: 2015-08-31T04:08:29Z
discontinued: false
dominant: false
experience: experience/None
group u list: _37_
m user id: 0
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status: status/Unavailable
bookmark: false
mutable description: reporter device for I14016. dominantly repressed by lambda cI
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BBa_B0010
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BBa_B0012
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BBa_B0034
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BBa_I14016
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BBa_E0030
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BBa_B0010
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BBa_B0012
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BBa_I14016
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BBa_E70202
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persistent identity: BBa E70202
display id: BBa_E70202
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Reporter for Dedicated Translation
creator: Bartholomew Canton
created: 2006-05-01T11:00:00Z
modified: 2015-08-31T04:07:27Z
discontinued: false
dominant: false
group u list: _11_6_
m user id: 0
owner id: 135
owning group id: 6
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
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component: BBa_B0012
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component: BBa_B0073
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BBa_R0040
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BBa_B0012
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BBa_E0040
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BBa_B0010
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BBa_B0073
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persistent identity: range1911782
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BBa_R0040
type: SequenceAnnotation
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BBa_E70203
type: ComponentDefinition
persistent identity: BBa E70203
display id: BBa_E70203
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Reporter for Dedicated Translation
creator: Bartholomew Canton
created: 2006-05-01T11:00:00Z
modified: 2015-08-31T04:07:27Z
discontinued: false
dominant: false
group u list: _11_6_
m user id: 0
owner id: 135
owning group id: 6
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_B0010
component: BBa_B0074
component: BBa_R0040
component: BBa_E0040
component: BBa_B0012
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sequence annotation: BBa_B0074
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sequence: https://synbiohub.org/public/igem/BBa_E70203_sequence/1

BBa_B0010
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access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0074
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display id: component1883612
version: 1
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access: public
definition: https://synbiohub.org/public/igem/BBa_B0074/1

BBa_R0040
type: Component
persistent identity: component1883608
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version: 1
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BBa_E0040
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persistent identity: component1883613
display id: component1883613
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definition: https://synbiohub.org/public/igem/BBa_E0040/1

BBa_B0012
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display id: component1883616
version: 1
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access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

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version: 1
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persistent identity: range1883612
display id: range1883612
version: 1
direction: direction/reverse
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orientation: inline

BBa_B0012
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display id: annotation1883616
version: 1
top level: BBa_E70203
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BBa_R0040
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BBa_B0010
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version: 1
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type: Range
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pLac+GFP
type: ComponentDefinition
persistent identity: BBa I763004
display id: BBa_I763004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: GFP coding device switched on by IPTG
creator: Alice Pasini
created: 2007-07-29T11:00:00Z
modified: 2015-08-31T04:08:08Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _111_
m user id: 0
owner id: 2088
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Contains IPTG inducible promoter, an RBS, GFP (+ LVA tag), and a terminator.
mutable notes: This part will be made using the standard ligation tecniques.
mutable provenance: Igem 2007 plates
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_B0010
component: BBa_B0034
component: BBa_B0012
component: BBa_R0010
component: BBa_J04031
sequence annotation: BBa_J04031
sequence annotation: BBa_B0034
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sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_I763004_sequence/1

BBa_B0010
type: Component
persistent identity: component1940247
display id: component1940247
version: 1
top level: pLac+GFP
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0034
type: Component
persistent identity: component1940241
display id: component1940241
version: 1
top level: pLac+GFP
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0012
type: Component
persistent identity: component1940249
display id: component1940249
version: 1
top level: pLac+GFP
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_R0010
type: Component
persistent identity: component1940233
display id: component1940233
version: 1
top level: pLac+GFP
access: public
definition: https://synbiohub.org/public/igem/BBa_R0010/1

BBa_J04031
type: Component
persistent identity: component1940246
display id: component1940246
version: 1
top level: pLac+GFP
access: public
definition: https://synbiohub.org/public/igem/BBa_J04031/1

BBa_J04031
type: SequenceAnnotation
persistent identity: annotation1940246
display id: annotation1940246
version: 1
top level: pLac+GFP
location: https://synbiohub.org/public/igem/BBa_I763004/annotation1940246/range1940246/1
component: BBa_J04031

https://synbiohub.org/public/igem/BBa_I763004/annotation1940246/range1940246/1
type: Range
persistent identity: range1940246
display id: range1940246
version: 1
direction: direction/reverse
top level: pLac+GFP
start: 227
end: 985
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1940241
display id: annotation1940241
version: 1
top level: pLac+GFP
location: https://synbiohub.org/public/igem/BBa_I763004/annotation1940241/range1940241/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I763004/annotation1940241/range1940241/1
type: Range
persistent identity: range1940241
display id: range1940241
version: 1
direction: direction/reverse
top level: pLac+GFP
start: 209
end: 220
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1940247
display id: annotation1940247
version: 1
top level: pLac+GFP
location: https://synbiohub.org/public/igem/BBa_I763004/annotation1940247/range1940247/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I763004/annotation1940247/range1940247/1
type: Range
persistent identity: range1940247
display id: range1940247
version: 1
direction: direction/forward
top level: pLac+GFP
start: 994
end: 1073
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation1940233
display id: annotation1940233
version: 1
top level: pLac+GFP
location: https://synbiohub.org/public/igem/BBa_I763004/annotation1940233/range1940233/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_I763004/annotation1940233/range1940233/1
type: Range
persistent identity: range1940233
display id: range1940233
version: 1
direction: direction/forward
top level: pLac+GFP
start: 1
end: 200
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1940249
display id: annotation1940249
version: 1
top level: pLac+GFP
location: https://synbiohub.org/public/igem/BBa_I763004/annotation1940249/range1940249/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I763004/annotation1940249/range1940249/1
type: Range
persistent identity: range1940249
display id: range1940249
version: 1
direction: direction/forward
top level: pLac+GFP
start: 1082
end: 1122
orientation: inline

BBa_J13026
type: ComponentDefinition
persistent identity: BBa J13026
display id: BBa_J13026
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: 3OC6HSL+LuxR dependent GFPm3 generator
creator: Jeff Tabor
created: 2005-07-17T11:00:00Z
modified: 2015-08-31T04:08:29Z
discontinued: false
dominant: false
experience: experience/None
group u list: _37_
m user id: 0
owner id: 88
owning group id: 37
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: dominantly repressed by lambda cI
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_B0010
component: BBa_B0012
component: BBa_R0065
component: BBa_B0034
component: BBa_E0040
sequence annotation: BBa_B0012
sequence annotation: BBa_E0040
sequence annotation: BBa_B0010
sequence annotation: BBa_R0065
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_J13026_sequence/1

BBa_B0010
type: Component
persistent identity: component1586462
display id: component1586462
version: 1
top level: BBa_J13026
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
type: Component
persistent identity: component1586472
display id: component1586472
version: 1
top level: BBa_J13026
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_R0065
type: Component
persistent identity: component1586443
display id: component1586443
version: 1
top level: BBa_J13026
access: public
definition: https://synbiohub.org/public/igem/BBa_R0065/1

BBa_B0034
type: Component
persistent identity: component1586455
display id: component1586455
version: 1
top level: BBa_J13026
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_E0040
type: Component
persistent identity: component1586457
display id: component1586457
version: 1
top level: BBa_J13026
access: public
definition: https://synbiohub.org/public/igem/BBa_E0040/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1586472
display id: annotation1586472
version: 1
top level: BBa_J13026
location: https://synbiohub.org/public/igem/BBa_J13026/annotation1586472/range1586472/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_J13026/annotation1586472/range1586472/1
type: Range
persistent identity: range1586472
display id: range1586472
version: 1
direction: direction/forward
top level: BBa_J13026
start: 940
end: 980
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation1586457
display id: annotation1586457
version: 1
top level: BBa_J13026
location: https://synbiohub.org/public/igem/BBa_J13026/annotation1586457/range1586457/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_J13026/annotation1586457/range1586457/1
type: Range
persistent identity: range1586457
display id: range1586457
version: 1
direction: direction/forward
top level: BBa_J13026
start: 124
end: 843
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1586462
display id: annotation1586462
version: 1
top level: BBa_J13026
location: https://synbiohub.org/public/igem/BBa_J13026/annotation1586462/range1586462/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_J13026/annotation1586462/range1586462/1
type: Range
persistent identity: range1586462
display id: range1586462
version: 1
direction: direction/forward
top level: BBa_J13026
start: 852
end: 931
orientation: inline

BBa_R0065
type: SequenceAnnotation
persistent identity: annotation1586443
display id: annotation1586443
version: 1
top level: BBa_J13026
location: https://synbiohub.org/public/igem/BBa_J13026/annotation1586443/range1586443/1
component: BBa_R0065

https://synbiohub.org/public/igem/BBa_J13026/annotation1586443/range1586443/1
type: Range
persistent identity: range1586443
display id: range1586443
version: 1
direction: direction/forward
top level: BBa_J13026
start: 1
end: 97
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1586455
display id: annotation1586455
version: 1
top level: BBa_J13026
location: https://synbiohub.org/public/igem/BBa_J13026/annotation1586455/range1586455/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_J13026/annotation1586455/range1586455/1
type: Range
persistent identity: range1586455
display id: range1586455
version: 1
direction: direction/forward
top level: BBa_J13026
start: 106
end: 117
orientation: inline

Prm+ YFP
type: ComponentDefinition
persistent identity: BBa S03466
display id: BBa_S03466
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Prm+ with YFP as a reporter
creator: ETH Zurich iGEM Team 2005
created: 2006-05-01T11:00:00Z
modified: 2015-05-08T01:14:23Z
discontinued: false
dominant: false
group u list: _34_
m user id: 0
owner id: 398
owning group id: 34
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Prm+ promoter of the lambda-system (with OR3 region deactivated over mutation) with YFP as a reporter. It is needed in subsequent parts to observe the performance of the complete input-system, which is based on the modified lambda-system.
mutable notes: For more details see Sensing Device and Event Processing Device in the ETH Team iGEM2005 WiKi documentation.
mutable provenance: Registry 7.05
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_B0010
component: BBa_B0034
component: BBa_E0032
component: BBa_B0012
component: BBa_I12007
sequence annotation: BBa_E0032
sequence annotation: BBa_B0012
sequence annotation: BBa_I12007
sequence annotation: BBa_B0034
sequence annotation: BBa_B0010
sequence: https://synbiohub.org/public/igem/BBa_S03466_sequence/1

BBa_B0010
type: Component
persistent identity: component1852383
display id: component1852383
version: 1
top level: Prm+ YFP
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0034
type: Component
persistent identity: component1852363
display id: component1852363
version: 1
top level: Prm+ YFP
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_E0032
type: Component
persistent identity: component1852375
display id: component1852375
version: 1
top level: Prm+ YFP
access: public
definition: https://synbiohub.org/public/igem/BBa_E0032/1

BBa_B0012
type: Component
persistent identity: component1852393
display id: component1852393
version: 1
top level: Prm+ YFP
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_I12007
type: Component
persistent identity: component1852358
display id: component1852358
version: 1
top level: Prm+ YFP
access: public
definition: https://synbiohub.org/public/igem/BBa_I12007/1

BBa_E0032
type: SequenceAnnotation
persistent identity: annotation1852375
display id: annotation1852375
version: 1
top level: Prm+ YFP
location: https://synbiohub.org/public/igem/BBa_S03466/annotation1852375/range1852375/1
component: BBa_E0032

https://synbiohub.org/public/igem/BBa_S03466/annotation1852375/range1852375/1
type: Range
persistent identity: range1852375
display id: range1852375
version: 1
direction: direction/forward
top level: Prm+ YFP
start: 109
end: 870
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1852393
display id: annotation1852393
version: 1
top level: Prm+ YFP
location: https://synbiohub.org/public/igem/BBa_S03466/annotation1852393/range1852393/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_S03466/annotation1852393/range1852393/1
type: Range
persistent identity: range1852393
display id: range1852393
version: 1
direction: direction/forward
top level: Prm+ YFP
start: 967
end: 1007
orientation: inline

BBa_I12007
type: SequenceAnnotation
persistent identity: annotation1852358
display id: annotation1852358
version: 1
top level: Prm+ YFP
location: https://synbiohub.org/public/igem/BBa_S03466/annotation1852358/range1852358/1
component: BBa_I12007

https://synbiohub.org/public/igem/BBa_S03466/annotation1852358/range1852358/1
type: Range
persistent identity: range1852358
display id: range1852358
version: 1
direction: direction/reverse
top level: Prm+ YFP
start: 1
end: 82
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1852363
display id: annotation1852363
version: 1
top level: Prm+ YFP
location: https://synbiohub.org/public/igem/BBa_S03466/annotation1852363/range1852363/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_S03466/annotation1852363/range1852363/1
type: Range
persistent identity: range1852363
display id: range1852363
version: 1
direction: direction/reverse
top level: Prm+ YFP
start: 91
end: 102
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1852383
display id: annotation1852383
version: 1
top level: Prm+ YFP
location: https://synbiohub.org/public/igem/BBa_S03466/annotation1852383/range1852383/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_S03466/annotation1852383/range1852383/1
type: Range
persistent identity: range1852383
display id: range1852383
version: 1
direction: direction/forward
top level: Prm+ YFP
start: 879
end: 958
orientation: inline

BBa_J22202
type: ComponentDefinition
persistent identity: BBa J22202
display id: BBa_J22202
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pLac-CFP
creator: Mukund Sugat Nilesh
created: 2007-06-20T11:00:00Z
modified: 2015-08-31T04:08:38Z
discontinued: false
dominant: false
experience: experience/None
group u list: _70_44_
m user id: 0
owner id: 439
owning group id: 70
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: a similar part is available in registry. we designed this as we faced some problems in CFP expression with the earlier part. we have tested this part and it is functional.
mutable notes: none.
mutable provenance: registry
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_B0010
component: BBa_B0034
component: BBa_B0012
component: BBa_R0011
component: BBa_E0020
sequence annotation: BBa_B0012
sequence annotation: BBa_R0011
sequence annotation: BBa_E0020
sequence annotation: BBa_B0034
sequence annotation: BBa_B0010
sequence: https://synbiohub.org/public/igem/BBa_J22202_sequence/1

BBa_B0010
type: Component
persistent identity: component1935029
display id: component1935029
version: 1
top level: BBa_J22202
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0034
type: Component
persistent identity: component1935027
display id: component1935027
version: 1
top level: BBa_J22202
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0012
type: Component
persistent identity: component1935031
display id: component1935031
version: 1
top level: BBa_J22202
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_R0011
type: Component
persistent identity: component1935021
display id: component1935021
version: 1
top level: BBa_J22202
access: public
definition: https://synbiohub.org/public/igem/BBa_R0011/1

BBa_E0020
type: Component
persistent identity: component1935028
display id: component1935028
version: 1
top level: BBa_J22202
access: public
definition: https://synbiohub.org/public/igem/BBa_E0020/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1935031
display id: annotation1935031
version: 1
top level: BBa_J22202
location: https://synbiohub.org/public/igem/BBa_J22202/annotation1935031/range1935031/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_J22202/annotation1935031/range1935031/1
type: Range
persistent identity: range1935031
display id: range1935031
version: 1
direction: direction/forward
top level: BBa_J22202
start: 901
end: 941
orientation: inline

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation1935021
display id: annotation1935021
version: 1
top level: BBa_J22202
location: https://synbiohub.org/public/igem/BBa_J22202/annotation1935021/range1935021/1
component: BBa_R0011

https://synbiohub.org/public/igem/BBa_J22202/annotation1935021/range1935021/1
type: Range
persistent identity: range1935021
display id: range1935021
version: 1
direction: direction/forward
top level: BBa_J22202
start: 1
end: 54
orientation: inline

BBa_E0020
type: SequenceAnnotation
persistent identity: annotation1935028
display id: annotation1935028
version: 1
top level: BBa_J22202
location: https://synbiohub.org/public/igem/BBa_J22202/annotation1935028/range1935028/1
component: BBa_E0020

https://synbiohub.org/public/igem/BBa_J22202/annotation1935028/range1935028/1
type: Range
persistent identity: range1935028
display id: range1935028
version: 1
direction: direction/reverse
top level: BBa_J22202
start: 82
end: 804
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1935027
display id: annotation1935027
version: 1
top level: BBa_J22202
location: https://synbiohub.org/public/igem/BBa_J22202/annotation1935027/range1935027/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_J22202/annotation1935027/range1935027/1
type: Range
persistent identity: range1935027
display id: range1935027
version: 1
direction: direction/reverse
top level: BBa_J22202
start: 64
end: 75
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1935029
display id: annotation1935029
version: 1
top level: BBa_J22202
location: https://synbiohub.org/public/igem/BBa_J22202/annotation1935029/range1935029/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_J22202/annotation1935029/range1935029/1
type: Range
persistent identity: range1935029
display id: range1935029
version: 1
direction: direction/forward
top level: BBa_J22202
start: 813
end: 892
orientation: inline

BBa_J47862
type: ComponentDefinition
persistent identity: BBa J47862
display id: BBa_J47862
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pLacI + ECFP (LVA+)
creator: Jon Badalamenti
created: 2006-10-26T11:00:00Z
modified: 2015-08-31T04:08:51Z
discontinued: false
dominant: false
group u list: _72_40_
m user id: 0
owner id: 475
owning group id: 72
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: R0010.B0031.E0022.B0015
mutable notes: none.
mutable provenance: This part was constructed from parts available through the registry.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_E0022
component: BBa_R0010
component: BBa_B0010
component: BBa_B0031
component: BBa_B0012
sequence annotation: BBa_E0022
sequence annotation: BBa_R0010
sequence annotation: BBa_B0031
sequence annotation: BBa_B0012
sequence annotation: BBa_B0010
sequence: https://synbiohub.org/public/igem/BBa_J47862_sequence/1

BBa_E0022
type: Component
persistent identity: component1907717
display id: component1907717
version: 1
top level: BBa_J47862
access: public
definition: https://synbiohub.org/public/igem/BBa_E0022/1

BBa_R0010
type: Component
persistent identity: component1907707
display id: component1907707
version: 1
top level: BBa_J47862
access: public
definition: https://synbiohub.org/public/igem/BBa_R0010/1

BBa_B0010
type: Component
persistent identity: component1907721
display id: component1907721
version: 1
top level: BBa_J47862
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0031
type: Component
persistent identity: component1907715
display id: component1907715
version: 1
top level: BBa_J47862
access: public
definition: https://synbiohub.org/public/igem/BBa_B0031/1

BBa_B0012
type: Component
persistent identity: component1907723
display id: component1907723
version: 1
top level: BBa_J47862
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_E0022
type: SequenceAnnotation
persistent identity: annotation1907717
display id: annotation1907717
version: 1
top level: BBa_J47862
location: https://synbiohub.org/public/igem/BBa_J47862/annotation1907717/range1907717/1
component: BBa_E0022

https://synbiohub.org/public/igem/BBa_J47862/annotation1907717/range1907717/1
type: Range
persistent identity: range1907717
display id: range1907717
version: 1
direction: direction/forward
top level: BBa_J47862
start: 229
end: 990
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation1907707
display id: annotation1907707
version: 1
top level: BBa_J47862
location: https://synbiohub.org/public/igem/BBa_J47862/annotation1907707/range1907707/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_J47862/annotation1907707/range1907707/1
type: Range
persistent identity: range1907707
display id: range1907707
version: 1
direction: direction/forward
top level: BBa_J47862
start: 1
end: 200
orientation: inline

BBa_B0031
type: SequenceAnnotation
persistent identity: annotation1907715
display id: annotation1907715
version: 1
top level: BBa_J47862
location: https://synbiohub.org/public/igem/BBa_J47862/annotation1907715/range1907715/1
component: BBa_B0031

https://synbiohub.org/public/igem/BBa_J47862/annotation1907715/range1907715/1
type: Range
persistent identity: range1907715
display id: range1907715
version: 1
direction: direction/reverse
top level: BBa_J47862
start: 209
end: 222
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1907723
display id: annotation1907723
version: 1
top level: BBa_J47862
location: https://synbiohub.org/public/igem/BBa_J47862/annotation1907723/range1907723/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_J47862/annotation1907723/range1907723/1
type: Range
persistent identity: range1907723
display id: range1907723
version: 1
direction: direction/forward
top level: BBa_J47862
start: 1087
end: 1127
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1907721
display id: annotation1907721
version: 1
top level: BBa_J47862
location: https://synbiohub.org/public/igem/BBa_J47862/annotation1907721/range1907721/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_J47862/annotation1907721/range1907721/1
type: Range
persistent identity: range1907721
display id: range1907721
version: 1
direction: direction/forward
top level: BBa_J47862
start: 999
end: 1078
orientation: inline

Pr+CFP-
type: ComponentDefinition
persistent identity: BBa S03475
display id: BBa_S03475
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Lambda-system Pr promoter with CFP(no degradation tag) reporter
creator: ETH Zurich iGEM Team 2005
created: 2006-05-01T11:00:00Z
modified: 2015-05-08T01:14:23Z
discontinued: false
dominant: false
group u list: _34_
m user id: 0
owner id: 398
owning group id: 34
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Testing Pr base level activity with specific reporter CFP without degradation tag for longer lifetime.
mutable notes: For more details see Sensing Device and Event Processing Device in the ETH Team iGEM2005 WiKi documentation.
mutable provenance: Registry 7.05
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_E0020
component: BBa_B0034
component: BBa_B0012
component: BBa_R0051
component: BBa_B0010
sequence annotation: BBa_B0034
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_E0020
sequence annotation: BBa_R0051
sequence: https://synbiohub.org/public/igem/BBa_S03475_sequence/1

BBa_E0020
type: Component
persistent identity: component1853198
display id: component1853198
version: 1
top level: Pr+CFP-
access: public
definition: https://synbiohub.org/public/igem/BBa_E0020/1

BBa_B0034
type: Component
persistent identity: component1853196
display id: component1853196
version: 1
top level: Pr+CFP-
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0012
type: Component
persistent identity: component1853213
display id: component1853213
version: 1
top level: Pr+CFP-
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_R0051
type: Component
persistent identity: component1853188
display id: component1853188
version: 1
top level: Pr+CFP-
access: public
definition: https://synbiohub.org/public/igem/BBa_R0051/1

BBa_B0010
type: Component
persistent identity: component1853203
display id: component1853203
version: 1
top level: Pr+CFP-
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1853196
display id: annotation1853196
version: 1
top level: Pr+CFP-
location: https://synbiohub.org/public/igem/BBa_S03475/annotation1853196/range1853196/1
component: BBa_B0034

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type: Range
persistent identity: range1853196
display id: range1853196
version: 1
direction: direction/reverse
top level: Pr+CFP-
start: 58
end: 69
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1853203
display id: annotation1853203
version: 1
top level: Pr+CFP-
location: https://synbiohub.org/public/igem/BBa_S03475/annotation1853203/range1853203/1
component: BBa_B0010

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type: Range
persistent identity: range1853203
display id: range1853203
version: 1
direction: direction/forward
top level: Pr+CFP-
start: 807
end: 886
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1853213
display id: annotation1853213
version: 1
top level: Pr+CFP-
location: https://synbiohub.org/public/igem/BBa_S03475/annotation1853213/range1853213/1
component: BBa_B0012

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type: Range
persistent identity: range1853213
display id: range1853213
version: 1
direction: direction/forward
top level: Pr+CFP-
start: 895
end: 935
orientation: inline

BBa_E0020
type: SequenceAnnotation
persistent identity: annotation1853198
display id: annotation1853198
version: 1
top level: Pr+CFP-
location: https://synbiohub.org/public/igem/BBa_S03475/annotation1853198/range1853198/1
component: BBa_E0020

https://synbiohub.org/public/igem/BBa_S03475/annotation1853198/range1853198/1
type: Range
persistent identity: range1853198
display id: range1853198
version: 1
direction: direction/reverse
top level: Pr+CFP-
start: 76
end: 798
orientation: inline

BBa_R0051
type: SequenceAnnotation
persistent identity: annotation1853188
display id: annotation1853188
version: 1
top level: Pr+CFP-
location: https://synbiohub.org/public/igem/BBa_S03475/annotation1853188/range1853188/1
component: BBa_R0051

https://synbiohub.org/public/igem/BBa_S03475/annotation1853188/range1853188/1
type: Range
persistent identity: range1853188
display id: range1853188
version: 1
direction: direction/forward
top level: Pr+CFP-
start: 1
end: 49
orientation: inline

BBa_I13603
type: ComponentDefinition
persistent identity: BBa I13603
display id: BBa_I13603
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Lac operator with CFP reporter (with LVA tag) [R/Lc+]
creator: Christopher Batten, Victoria Chou, Kenneth Nesmith
created: 2004-07-15T11:00:00Z
modified: 2015-08-31T04:07:36Z
discontinued: false
dominant: true
experience: experience/None
group u list: _6_
m user id: 0
owner id: 101
owning group id: 7
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_R0011
component: BBa_E0422
sequence annotation: BBa_E0422
sequence annotation: BBa_R0011
sequence: https://synbiohub.org/public/igem/BBa_I13603_sequence/1

BBa_R0011
type: Component
persistent identity: component2221478
display id: component2221478
version: 1
top level: BBa_I13603
access: public
definition: https://synbiohub.org/public/igem/BBa_R0011/1

BBa_E0422
type: Component
persistent identity: component2221496
display id: component2221496
version: 1
top level: BBa_I13603
access: public
definition: https://synbiohub.org/public/igem/BBa_E0422/1

BBa_E0422
type: SequenceAnnotation
persistent identity: annotation2221496
display id: annotation2221496
version: 1
top level: BBa_I13603
location: https://synbiohub.org/public/igem/BBa_I13603/annotation2221496/range2221496/1
component: BBa_E0422

https://synbiohub.org/public/igem/BBa_I13603/annotation2221496/range2221496/1
type: Range
persistent identity: range2221496
display id: range2221496
version: 1
direction: direction/reverse
top level: BBa_I13603
start: 64
end: 980
orientation: inline

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation2221478
display id: annotation2221478
version: 1
top level: BBa_I13603
location: https://synbiohub.org/public/igem/BBa_I13603/annotation2221478/range2221478/1
component: BBa_R0011

https://synbiohub.org/public/igem/BBa_I13603/annotation2221478/range2221478/1
type: Range
persistent identity: range2221478
display id: range2221478
version: 1
direction: direction/forward
top level: BBa_I13603
start: 1
end: 54
orientation: inline

BBa_I741102
type: ComponentDefinition
persistent identity: BBa I741102
display id: BBa_I741102
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter (I741018) and GFP (E0240)
creator: Lucien Weiss
created: 2007-06-21T11:00:00Z
modified: 2015-08-31T04:08:02Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _143_
m user id: 0
owner id: 1763
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_B0030
component: BBa_B0012
component: BBa_E0040
component: BBa_B0010
component: BBa_I741018
sequence annotation: BBa_I741018
sequence annotation: BBa_E0040
sequence annotation: BBa_B0030
sequence annotation: BBa_B0012
sequence annotation: BBa_B0010
sequence: https://synbiohub.org/public/igem/BBa_I741102_sequence/1

BBa_B0030
type: Component
persistent identity: component1935257
display id: component1935257
version: 1
top level: BBa_I741102
access: public
definition: https://synbiohub.org/public/igem/BBa_B0030/1

BBa_B0012
type: Component
persistent identity: component1935263
display id: component1935263
version: 1
top level: BBa_I741102
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_E0040
type: Component
persistent identity: component1935260
display id: component1935260
version: 1
top level: BBa_I741102
access: public
definition: https://synbiohub.org/public/igem/BBa_E0040/1

BBa_B0010
type: Component
persistent identity: component1935261
display id: component1935261
version: 1
top level: BBa_I741102
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_I741018
type: Component
persistent identity: component1935255
display id: component1935255
version: 1
top level: BBa_I741102
access: public
definition: https://synbiohub.org/public/igem/BBa_I741018/1

BBa_I741018
type: SequenceAnnotation
persistent identity: annotation1935255
display id: annotation1935255
version: 1
top level: BBa_I741102
location: https://synbiohub.org/public/igem/BBa_I741102/annotation1935255/range1935255/1
component: BBa_I741018

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type: Range
persistent identity: range1935255
display id: range1935255
version: 1
direction: direction/reverse
top level: BBa_I741102
start: 1
end: 221
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation1935260
display id: annotation1935260
version: 1
top level: BBa_I741102
location: https://synbiohub.org/public/igem/BBa_I741102/annotation1935260/range1935260/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_I741102/annotation1935260/range1935260/1
type: Range
persistent identity: range1935260
display id: range1935260
version: 1
direction: direction/reverse
top level: BBa_I741102
start: 251
end: 970
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation1935257
display id: annotation1935257
version: 1
top level: BBa_I741102
location: https://synbiohub.org/public/igem/BBa_I741102/annotation1935257/range1935257/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_I741102/annotation1935257/range1935257/1
type: Range
persistent identity: range1935257
display id: range1935257
version: 1
direction: direction/forward
top level: BBa_I741102
start: 230
end: 244
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1935263
display id: annotation1935263
version: 1
top level: BBa_I741102
location: https://synbiohub.org/public/igem/BBa_I741102/annotation1935263/range1935263/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I741102/annotation1935263/range1935263/1
type: Range
persistent identity: range1935263
display id: range1935263
version: 1
direction: direction/forward
top level: BBa_I741102
start: 1067
end: 1107
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1935261
display id: annotation1935261
version: 1
top level: BBa_I741102
location: https://synbiohub.org/public/igem/BBa_I741102/annotation1935261/range1935261/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I741102/annotation1935261/range1935261/1
type: Range
persistent identity: range1935261
display id: range1935261
version: 1
direction: direction/forward
top level: BBa_I741102
start: 979
end: 1058
orientation: inline

p(osmY).GF
type: ComponentDefinition
persistent identity: BBa J45995
display id: BBa_J45995
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Stationary phase dependent GFP generator
creator: Stephen Payne
created: 2006-10-30T12:00:00Z
modified: 2015-08-31T04:08:50Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _84_
m user id: 0
owner id: 642
owning group id: 84
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This part is a composite part consisting of J45992 (a stationary phase promoter) and E0840 (a GFP generator). Thus, this device creates green fluorescent protein in stationary phase.
mutable notes: This part was mainly designed to test whether J45250 would smell like banana only in stationary phase.
mutable provenance: J45992 was PCRed out of the E. coli genome, while E0840 was constructed at the Registry of Standard Biological Parts.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0030
component: BBa_E0040
component: BBa_B0012
component: BBa_J45992
component: BBa_B0010
sequence annotation: BBa_B0010
sequence annotation: BBa_E0040
sequence annotation: BBa_B0012
sequence annotation: BBa_J45992
sequence annotation: BBa_B0030
sequence: https://synbiohub.org/public/igem/BBa_J45995_sequence/1

BBa_B0030
type: Component
persistent identity: component1909183
display id: component1909183
version: 1
top level: p(osmY).GF
access: public
definition: https://synbiohub.org/public/igem/BBa_B0030/1

BBa_E0040
type: Component
persistent identity: component1909185
display id: component1909185
version: 1
top level: p(osmY).GF
access: public
definition: https://synbiohub.org/public/igem/BBa_E0040/1

BBa_B0012
type: Component
persistent identity: component1909188
display id: component1909188
version: 1
top level: p(osmY).GF
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_J45992
type: Component
persistent identity: component1909181
display id: component1909181
version: 1
top level: p(osmY).GF
access: public
definition: https://synbiohub.org/public/igem/BBa_J45992/1

BBa_B0010
type: Component
persistent identity: component1909186
display id: component1909186
version: 1
top level: p(osmY).GF
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1909186
display id: annotation1909186
version: 1
top level: p(osmY).GF
location: https://synbiohub.org/public/igem/BBa_J45995/annotation1909186/range1909186/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_J45995/annotation1909186/range1909186/1
type: Range
persistent identity: range1909186
display id: range1909186
version: 1
direction: direction/forward
top level: p(osmY).GF
start: 957
end: 1036
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation1909185
display id: annotation1909185
version: 1
top level: p(osmY).GF
location: https://synbiohub.org/public/igem/BBa_J45995/annotation1909185/range1909185/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_J45995/annotation1909185/range1909185/1
type: Range
persistent identity: range1909185
display id: range1909185
version: 1
direction: direction/reverse
top level: p(osmY).GF
start: 229
end: 948
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1909188
display id: annotation1909188
version: 1
top level: p(osmY).GF
location: https://synbiohub.org/public/igem/BBa_J45995/annotation1909188/range1909188/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_J45995/annotation1909188/range1909188/1
type: Range
persistent identity: range1909188
display id: range1909188
version: 1
direction: direction/forward
top level: p(osmY).GF
start: 1045
end: 1085
orientation: inline

BBa_J45992
type: SequenceAnnotation
persistent identity: annotation1909181
display id: annotation1909181
version: 1
top level: p(osmY).GF
location: https://synbiohub.org/public/igem/BBa_J45995/annotation1909181/range1909181/1
component: BBa_J45992

https://synbiohub.org/public/igem/BBa_J45995/annotation1909181/range1909181/1
type: Range
persistent identity: range1909181
display id: range1909181
version: 1
direction: direction/reverse
top level: p(osmY).GF
start: 1
end: 199
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation1909183
display id: annotation1909183
version: 1
top level: p(osmY).GF
location: https://synbiohub.org/public/igem/BBa_J45995/annotation1909183/range1909183/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_J45995/annotation1909183/range1909183/1
type: Range
persistent identity: range1909183
display id: range1909183
version: 1
direction: direction/forward
top level: p(osmY).GF
start: 208
end: 222
orientation: inline

Prm+RFP
type: ComponentDefinition
persistent identity: BBa S03337
display id: BBa_S03337
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Prm+ with RFP as a reporter
creator: ETH Zurich iGEM Team 2005
created: 2005-09-20T11:00:00Z
modified: 2015-05-08T01:14:21Z
discontinued: false
dominant: false
experience: experience/None
group u list: _34_
m user id: 0
owner id: 398
owning group id: 34
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Prm+ promoter of the lambda-system (with OR3 region deactivated over mutation) with RFP as a reporter. It is needed in subsequent parts to observe the performance of the complete input-system, which is based on the modified lambda-system.
mutable notes: For more details see Sensing Device and Event Processing Device documentation.
mutable provenance: Registry 7.05
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_I12007
component: BBa_B0034
component: BBa_E1010
component: BBa_B0010
component: BBa_B0012
sequence annotation: BBa_I12007
sequence annotation: BBa_E1010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_S03337_sequence/1

BBa_I12007
type: Component
persistent identity: component1700396
display id: component1700396
version: 1
top level: Prm+RFP
access: public
definition: https://synbiohub.org/public/igem/BBa_I12007/1

BBa_B0034
type: Component
persistent identity: component1700401
display id: component1700401
version: 1
top level: Prm+RFP
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_E1010
type: Component
persistent identity: component1700408
display id: component1700408
version: 1
top level: Prm+RFP
access: public
definition: https://synbiohub.org/public/igem/BBa_E1010/1

BBa_B0010
type: Component
persistent identity: component1700414
display id: component1700414
version: 1
top level: Prm+RFP
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
type: Component
persistent identity: component1700424
display id: component1700424
version: 1
top level: Prm+RFP
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_I12007
type: SequenceAnnotation
persistent identity: annotation1700396
display id: annotation1700396
version: 1
top level: Prm+RFP
location: https://synbiohub.org/public/igem/BBa_S03337/annotation1700396/range1700396/1
component: BBa_I12007

https://synbiohub.org/public/igem/BBa_S03337/annotation1700396/range1700396/1
type: Range
persistent identity: range1700396
display id: range1700396
version: 1
direction: direction/forward
top level: Prm+RFP
start: 1
end: 82
orientation: inline

BBa_E1010
type: SequenceAnnotation
persistent identity: annotation1700408
display id: annotation1700408
version: 1
top level: Prm+RFP
location: https://synbiohub.org/public/igem/BBa_S03337/annotation1700408/range1700408/1
component: BBa_E1010

https://synbiohub.org/public/igem/BBa_S03337/annotation1700408/range1700408/1
type: Range
persistent identity: range1700408
display id: range1700408
version: 1
direction: direction/forward
top level: Prm+RFP
start: 109
end: 789
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1700414
display id: annotation1700414
version: 1
top level: Prm+RFP
location: https://synbiohub.org/public/igem/BBa_S03337/annotation1700414/range1700414/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_S03337/annotation1700414/range1700414/1
type: Range
persistent identity: range1700414
display id: range1700414
version: 1
direction: direction/forward
top level: Prm+RFP
start: 823
end: 902
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1700424
display id: annotation1700424
version: 1
top level: Prm+RFP
location: https://synbiohub.org/public/igem/BBa_S03337/annotation1700424/range1700424/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_S03337/annotation1700424/range1700424/1
type: Range
persistent identity: range1700424
display id: range1700424
version: 1
direction: direction/forward
top level: Prm+RFP
start: 911
end: 951
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1700401
display id: annotation1700401
version: 1
top level: Prm+RFP
location: https://synbiohub.org/public/igem/BBa_S03337/annotation1700401/range1700401/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_S03337/annotation1700401/range1700401/1
type: Range
persistent identity: range1700401
display id: range1700401
version: 1
direction: direction/forward
top level: Prm+RFP
start: 91
end: 102
orientation: inline

Prm+ CFP
type: ComponentDefinition
persistent identity: BBa S03465
display id: BBa_S03465
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Prm+ with CFP as a reporter
creator: ETH Zurich iGEM Team 2005
created: 2006-05-01T11:00:00Z
modified: 2015-05-08T01:14:23Z
discontinued: false
dominant: false
group u list: _34_
m user id: 0
owner id: 398
owning group id: 34
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Prm+ promoter of the lambda-system (with OR3 region deactivated over mutation) with a CFP reporter. The purpose is to roughly quantify its relative basal activity compared with the constitutive activity of the Pr promoter.
mutable notes: For more details see Sensing Device and Event Processing Device in the ETH Team iGEM2005 WiKi documentation.
mutable provenance: Registry 7.05
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_E0022
component: BBa_B0012
component: BBa_B0034
component: BBa_B0010
component: BBa_I12007
sequence annotation: BBa_B0012
sequence annotation: BBa_B0034
sequence annotation: BBa_B0010
sequence annotation: BBa_I12007
sequence annotation: BBa_E0022
sequence: https://synbiohub.org/public/igem/BBa_S03465_sequence/1

BBa_E0022
type: Component
persistent identity: component1852270
display id: component1852270
version: 1
top level: Prm+ CFP
access: public
definition: https://synbiohub.org/public/igem/BBa_E0022/1

BBa_B0012
type: Component
persistent identity: component1852288
display id: component1852288
version: 1
top level: Prm+ CFP
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0034
type: Component
persistent identity: component1852258
display id: component1852258
version: 1
top level: Prm+ CFP
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0010
type: Component
persistent identity: component1852278
display id: component1852278
version: 1
top level: Prm+ CFP
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_I12007
type: Component
persistent identity: component1852253
display id: component1852253
version: 1
top level: Prm+ CFP
access: public
definition: https://synbiohub.org/public/igem/BBa_I12007/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1852288
display id: annotation1852288
version: 1
top level: Prm+ CFP
location: https://synbiohub.org/public/igem/BBa_S03465/annotation1852288/range1852288/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_S03465/annotation1852288/range1852288/1
type: Range
persistent identity: range1852288
display id: range1852288
version: 1
direction: direction/forward
top level: Prm+ CFP
start: 967
end: 1007
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1852258
display id: annotation1852258
version: 1
top level: Prm+ CFP
location: https://synbiohub.org/public/igem/BBa_S03465/annotation1852258/range1852258/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_S03465/annotation1852258/range1852258/1
type: Range
persistent identity: range1852258
display id: range1852258
version: 1
direction: direction/reverse
top level: Prm+ CFP
start: 91
end: 102
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1852278
display id: annotation1852278
version: 1
top level: Prm+ CFP
location: https://synbiohub.org/public/igem/BBa_S03465/annotation1852278/range1852278/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_S03465/annotation1852278/range1852278/1
type: Range
persistent identity: range1852278
display id: range1852278
version: 1
direction: direction/forward
top level: Prm+ CFP
start: 879
end: 958
orientation: inline

BBa_I12007
type: SequenceAnnotation
persistent identity: annotation1852253
display id: annotation1852253
version: 1
top level: Prm+ CFP
location: https://synbiohub.org/public/igem/BBa_S03465/annotation1852253/range1852253/1
component: BBa_I12007

https://synbiohub.org/public/igem/BBa_S03465/annotation1852253/range1852253/1
type: Range
persistent identity: range1852253
display id: range1852253
version: 1
direction: direction/reverse
top level: Prm+ CFP
start: 1
end: 82
orientation: inline

BBa_E0022
type: SequenceAnnotation
persistent identity: annotation1852270
display id: annotation1852270
version: 1
top level: Prm+ CFP
location: https://synbiohub.org/public/igem/BBa_S03465/annotation1852270/range1852270/1
component: BBa_E0022

https://synbiohub.org/public/igem/BBa_S03465/annotation1852270/range1852270/1
type: Range
persistent identity: range1852270
display id: range1852270
version: 1
direction: direction/forward
top level: Prm+ CFP
start: 109
end: 870
orientation: inline

BBa_J58102
type: ComponentDefinition
persistent identity: BBa J58102
display id: BBa_J58102
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter activated by OmpR-P with the reporter GFP
creator: Valencia iGEM 2006
created: 2006-10-25T11:00:00Z
modified: 2015-08-31T03:14:48Z
discontinued: false
dominant: false
experience: experience/None
group u list: _83_
m user id: 0
owner id: 1100
owning group id: 83
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: Many signalling cellular processes occur using a phosphorylation mechanism. In order to assemble a transmembranal pathway, that frees a phosphore, with a genetic network within the cell, we use the promoter pOmpR (R0082). In E. coli, the osmosensing machinery using the EnvZ membrane protein follows this mechanism. That promoter is regulated negatively (repressed) by the transcription factor OmpR-P. We put a well known protein downstream: GFP (E0040) with a RBS (B0034) and a Terminator (B0015). This composite part will be very useful to characterize many cellular biosensors.
mutable notes: none
mutable provenance: To make this composite part we assemby two parts from the Registry (a promoter (R0082) and a generator (I13504)).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_R0082
component: BBa_E0040
component: BBa_B0012
component: BBa_B0010
component: BBa_B0034
sequence annotation: BBa_R0082
sequence annotation: BBa_E0040
sequence annotation: BBa_B0010
sequence annotation: BBa_B0034
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_J58102_sequence/1

BBa_R0082
type: Component
persistent identity: component1905114
display id: component1905114
version: 1
top level: BBa_J58102
access: public
definition: https://synbiohub.org/public/igem/BBa_R0082/1

BBa_E0040
type: Component
persistent identity: component1905117
display id: component1905117
version: 1
top level: BBa_J58102
access: public
definition: https://synbiohub.org/public/igem/BBa_E0040/1

BBa_B0012
type: Component
persistent identity: component1905120
display id: component1905120
version: 1
top level: BBa_J58102
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0010
type: Component
persistent identity: component1905118
display id: component1905118
version: 1
top level: BBa_J58102
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0034
type: Component
persistent identity: component1905116
display id: component1905116
version: 1
top level: BBa_J58102
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_R0082
type: SequenceAnnotation
persistent identity: annotation1905114
display id: annotation1905114
version: 1
top level: BBa_J58102
location: https://synbiohub.org/public/igem/BBa_J58102/annotation1905114/range1905114/1
component: BBa_R0082

https://synbiohub.org/public/igem/BBa_J58102/annotation1905114/range1905114/1
type: Range
persistent identity: range1905114
display id: range1905114
version: 1
direction: direction/reverse
top level: BBa_J58102
start: 1
end: 108
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation1905117
display id: annotation1905117
version: 1
top level: BBa_J58102
location: https://synbiohub.org/public/igem/BBa_J58102/annotation1905117/range1905117/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_J58102/annotation1905117/range1905117/1
type: Range
persistent identity: range1905117
display id: range1905117
version: 1
direction: direction/reverse
top level: BBa_J58102
start: 135
end: 854
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1905118
display id: annotation1905118
version: 1
top level: BBa_J58102
location: https://synbiohub.org/public/igem/BBa_J58102/annotation1905118/range1905118/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_J58102/annotation1905118/range1905118/1
type: Range
persistent identity: range1905118
display id: range1905118
version: 1
direction: direction/forward
top level: BBa_J58102
start: 863
end: 942
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1905116
display id: annotation1905116
version: 1
top level: BBa_J58102
location: https://synbiohub.org/public/igem/BBa_J58102/annotation1905116/range1905116/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_J58102/annotation1905116/range1905116/1
type: Range
persistent identity: range1905116
display id: range1905116
version: 1
direction: direction/reverse
top level: BBa_J58102
start: 117
end: 128
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1905120
display id: annotation1905120
version: 1
top level: BBa_J58102
location: https://synbiohub.org/public/igem/BBa_J58102/annotation1905120/range1905120/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_J58102/annotation1905120/range1905120/1
type: Range
persistent identity: range1905120
display id: range1905120
version: 1
direction: direction/forward
top level: BBa_J58102
start: 951
end: 991
orientation: inline

BBa_K1399012
type: ComponentDefinition
persistent identity: BBa K1399012
display id: BBa_K1399012
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Plac-RFP(LAA)
creator: Anna Stikane
created: 2014-09-18T11:00:00Z
modified: 2015-05-08T01:10:16Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1777_
m user id: 0
owner id: 22477
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: Lactose/IPTG inducible promoter with RBS, RFP reporter tagged with LAA-ssrA degradation tag and terminator. The tagged RFP is actively degraded within cell, thus provides better temporal resolution of red fluorescence and promoter activity.
mutable notes: Part was assembled using BrickClip assembly (BBF RFC 104) using other biobrick parts as templates. BrickClip assembly is a special case of more general Paperclip assembly method.[1]
mutable provenance: other biobricks
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: stop
sequence annotation: -35
sequence annotation: BBa_B0012
sequence annotation: polya
sequence annotation: RFP
sequence annotation: start
sequence annotation: BBa_B0034
sequence annotation: conserved
sequence annotation: tag
sequence annotation: BBa_B0015
sequence annotation: LacI binding site
sequence annotation: CAP binding site
sequence annotation: end of LacI coding region (inactive)
sequence annotation: BBa_R0010
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence annotation: T7 TE
sequence annotation: -10
sequence annotation: barcode from BBa_E1010
sequence annotation: BBa_K1399002
sequence: https://synbiohub.org/public/igem/BBa_K1399012_sequence/1

stop
type: SequenceAnnotation
persistent identity: annotation2383998
display id: annotation2383998
version: 1
top level: BBa_K1399012
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1399012/annotation2383998/range2383998/1

https://synbiohub.org/public/igem/BBa_K1399012/annotation2383998/range2383998/1
type: Range
persistent identity: range2383998
display id: range2383998
version: 1
direction: direction/forward
top level: BBa_K1399012
start: 935
end: 937
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2383989
display id: annotation2383989
version: 1
top level: BBa_K1399012
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1399012/annotation2383989/range2383989/1

https://synbiohub.org/public/igem/BBa_K1399012/annotation2383989/range2383989/1
type: Range
persistent identity: range2383989
display id: range2383989
version: 1
direction: direction/forward
top level: BBa_K1399012
start: 137
end: 142
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2384003
display id: annotation2384003
version: 1
top level: BBa_K1399012
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1399012/annotation2384003/range2384003/1

https://synbiohub.org/public/igem/BBa_K1399012/annotation2384003/range2384003/1
type: Range
persistent identity: range2384003
display id: range2384003
version: 1
direction: direction/forward
top level: BBa_K1399012
start: 1062
end: 1102
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation2384005
display id: annotation2384005
version: 1
top level: BBa_K1399012
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_K1399012/annotation2384005/range2384005/1

https://synbiohub.org/public/igem/BBa_K1399012/annotation2384005/range2384005/1
type: Range
persistent identity: range2384005
display id: range2384005
version: 1
direction: direction/forward
top level: BBa_K1399012
start: 1089
end: 1102
orientation: inline

RFP
type: SequenceAnnotation
persistent identity: annotation2383996
display id: annotation2383996
version: 1
top level: BBa_K1399012
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1399012/annotation2383996/range2383996/1

https://synbiohub.org/public/igem/BBa_K1399012/annotation2383996/range2383996/1
type: Range
persistent identity: range2383996
display id: range2383996
version: 1
direction: direction/forward
top level: BBa_K1399012
start: 230
end: 901
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2383992
display id: annotation2383992
version: 1
top level: BBa_K1399012
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1399012/annotation2383992/range2383992/1

https://synbiohub.org/public/igem/BBa_K1399012/annotation2383992/range2383992/1
type: Range
persistent identity: range2383992
display id: range2383992
version: 1
direction: direction/forward
top level: BBa_K1399012
start: 173
end: 173
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2383994
display id: annotation2383994
version: 1
top level: BBa_K1399012
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1399012/annotation2383994/range2383994/1

https://synbiohub.org/public/igem/BBa_K1399012/annotation2383994/range2383994/1
type: Range
persistent identity: range2383994
display id: range2383994
version: 1
direction: direction/reverse
top level: BBa_K1399012
start: 209
end: 220
orientation: inline

conserved
type: SequenceAnnotation
persistent identity: annotation2383993
display id: annotation2383993
version: 1
top level: BBa_K1399012
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K1399012/annotation2383993/range2383993/1

https://synbiohub.org/public/igem/BBa_K1399012/annotation2383993/range2383993/1
type: Range
persistent identity: range2383993
display id: range2383993
version: 1
direction: direction/forward
top level: BBa_K1399012
start: 213
end: 216
orientation: inline

tag
type: SequenceAnnotation
persistent identity: annotation2383997
display id: annotation2383997
version: 1
top level: BBa_K1399012
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1399012/annotation2383997/range2383997/1

https://synbiohub.org/public/igem/BBa_K1399012/annotation2383997/range2383997/1
type: Range
persistent identity: range2383997
display id: range2383997
version: 1
direction: direction/forward
top level: BBa_K1399012
start: 902
end: 934
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2384007
display id: annotation2384007
version: 1
top level: BBa_K1399012
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1399012/annotation2384007/range2384007/1

https://synbiohub.org/public/igem/BBa_K1399012/annotation2384007/range2384007/1
type: Range
persistent identity: range2384007
display id: range2384007
version: 1
direction: direction/reverse
top level: BBa_K1399012
start: 974
end: 1102
orientation: inline

LacI binding site
type: SequenceAnnotation
persistent identity: annotation2383991
display id: annotation2383991
version: 1
top level: BBa_K1399012
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1399012/annotation2383991/range2383991/1

https://synbiohub.org/public/igem/BBa_K1399012/annotation2383991/range2383991/1
type: Range
persistent identity: range2383991
display id: range2383991
version: 1
direction: direction/forward
top level: BBa_K1399012
start: 166
end: 200
orientation: inline

CAP binding site
type: SequenceAnnotation
persistent identity: annotation2383988
display id: annotation2383988
version: 1
top level: BBa_K1399012
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1399012/annotation2383988/range2383988/1

https://synbiohub.org/public/igem/BBa_K1399012/annotation2383988/range2383988/1
type: Range
persistent identity: range2383988
display id: range2383988
version: 1
direction: direction/forward
top level: BBa_K1399012
start: 89
end: 126
orientation: inline

end of LacI coding region (inactive)
type: SequenceAnnotation
persistent identity: annotation2383987
display id: annotation2383987
version: 1
top level: BBa_K1399012
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1399012/annotation2383987/range2383987/1

https://synbiohub.org/public/igem/BBa_K1399012/annotation2383987/range2383987/1
type: Range
persistent identity: range2383987
display id: range2383987
version: 1
direction: direction/forward
top level: BBa_K1399012
start: 1
end: 88
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation2383986
display id: annotation2383986
version: 1
top level: BBa_K1399012
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1399012/annotation2383986/range2383986/1

https://synbiohub.org/public/igem/BBa_K1399012/annotation2383986/range2383986/1
type: Range
persistent identity: range2383986
display id: range2383986
version: 1
direction: direction/forward
top level: BBa_K1399012
start: 1
end: 200
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2384001
display id: annotation2384001
version: 1
top level: BBa_K1399012
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1399012/annotation2384001/range2384001/1

https://synbiohub.org/public/igem/BBa_K1399012/annotation2384001/range2384001/1
type: Range
persistent identity: range2384001
display id: range2384001
version: 1
direction: direction/forward
top level: BBa_K1399012
start: 974
end: 1053
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation2384002
display id: annotation2384002
version: 1
top level: BBa_K1399012
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1399012/annotation2384002/range2384002/1

https://synbiohub.org/public/igem/BBa_K1399012/annotation2384002/range2384002/1
type: Range
persistent identity: range2384002
display id: range2384002
version: 1
direction: direction/forward
top level: BBa_K1399012
start: 985
end: 1028
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation2384004
display id: annotation2384004
version: 1
top level: BBa_K1399012
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1399012/annotation2384004/range2384004/1

https://synbiohub.org/public/igem/BBa_K1399012/annotation2384004/range2384004/1
type: Range
persistent identity: range2384004
display id: range2384004
version: 1
direction: direction/forward
top level: BBa_K1399012
start: 1069
end: 1088
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2383995
display id: annotation2383995
version: 1
top level: BBa_K1399012
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1399012/annotation2383995/range2383995/1

https://synbiohub.org/public/igem/BBa_K1399012/annotation2383995/range2383995/1
type: Range
persistent identity: range2383995
display id: range2383995
version: 1
direction: direction/forward
top level: BBa_K1399012
start: 227
end: 229
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2383990
display id: annotation2383990
version: 1
top level: BBa_K1399012
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1399012/annotation2383990/range2383990/1

https://synbiohub.org/public/igem/BBa_K1399012/annotation2383990/range2383990/1
type: Range
persistent identity: range2383990
display id: range2383990
version: 1
direction: direction/forward
top level: BBa_K1399012
start: 161
end: 166
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2383999
display id: annotation2383999
version: 1
top level: BBa_K1399012
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1399012/annotation2383999/range2383999/1

https://synbiohub.org/public/igem/BBa_K1399012/annotation2383999/range2383999/1
type: Range
persistent identity: range2383999
display id: range2383999
version: 1
direction: direction/forward
top level: BBa_K1399012
start: 938
end: 940
orientation: inline

barcode from BBa_E1010
type: SequenceAnnotation
persistent identity: annotation2386312
display id: annotation2386312
version: 1
top level: BBa_K1399012
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1399012/annotation2386312/range2386312/1

https://synbiohub.org/public/igem/BBa_K1399012/annotation2386312/range2386312/1
type: Range
persistent identity: range2386312
display id: range2386312
version: 1
direction: direction/forward
top level: BBa_K1399012
start: 941
end: 965
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2384006
display id: annotation2384006
version: 1
top level: BBa_K1399012
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1399012/annotation2384006/range2384006/1

https://synbiohub.org/public/igem/BBa_K1399012/annotation2384006/range2384006/1
type: Range
persistent identity: range2384006
display id: range2384006
version: 1
direction: direction/forward
top level: BBa_K1399012
start: 1095
end: 1095
orientation: inline

BBa_K1399002
type: SequenceAnnotation
persistent identity: annotation2384000
display id: annotation2384000
version: 1
top level: BBa_K1399012
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1399012/annotation2384000/range2384000/1

https://synbiohub.org/public/igem/BBa_K1399012/annotation2384000/range2384000/1
type: Range
persistent identity: range2384000
display id: range2384000
version: 1
direction: direction/reverse
top level: BBa_K1399012
start: 227
end: 940
orientation: inline

Pr+YFP-
type: ComponentDefinition
persistent identity: BBa S03476
display id: BBa_S03476
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Lambda-system Pr promoter with YFP(no degradation tag) reporter
creator: ETH Zurich iGEM Team 2005
created: 2006-05-01T11:00:00Z
modified: 2015-05-08T01:14:23Z
discontinued: false
dominant: false
group u list: _34_
m user id: 0
owner id: 398
owning group id: 34
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Testing Pr base level activity with specific reporter YFP without degradation tag for longer lifetime.
mutable notes: For more details see Sensing Device and Event Processing Device in the ETH Team iGEM2005 WiKi documentation.
mutable provenance: Registry 7.05
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_R0051
component: BBa_B0010
component: BBa_E0030
component: BBa_B0012
component: BBa_B0034
sequence annotation: BBa_R0051
sequence annotation: BBa_B0034
sequence annotation: BBa_B0012
sequence annotation: BBa_E0030
sequence annotation: BBa_B0010
sequence: https://synbiohub.org/public/igem/BBa_S03476_sequence/1

BBa_R0051
type: Component
persistent identity: component1853272
display id: component1853272
version: 1
top level: Pr+YFP-
access: public
definition: https://synbiohub.org/public/igem/BBa_R0051/1

BBa_B0010
type: Component
persistent identity: component1853287
display id: component1853287
version: 1
top level: Pr+YFP-
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_E0030
type: Component
persistent identity: component1853282
display id: component1853282
version: 1
top level: Pr+YFP-
access: public
definition: https://synbiohub.org/public/igem/BBa_E0030/1

BBa_B0012
type: Component
persistent identity: component1853297
display id: component1853297
version: 1
top level: Pr+YFP-
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0034
type: Component
persistent identity: component1853280
display id: component1853280
version: 1
top level: Pr+YFP-
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_R0051
type: SequenceAnnotation
persistent identity: annotation1853272
display id: annotation1853272
version: 1
top level: Pr+YFP-
location: https://synbiohub.org/public/igem/BBa_S03476/annotation1853272/range1853272/1
component: BBa_R0051

https://synbiohub.org/public/igem/BBa_S03476/annotation1853272/range1853272/1
type: Range
persistent identity: range1853272
display id: range1853272
version: 1
direction: direction/forward
top level: Pr+YFP-
start: 1
end: 49
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1853280
display id: annotation1853280
version: 1
top level: Pr+YFP-
location: https://synbiohub.org/public/igem/BBa_S03476/annotation1853280/range1853280/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_S03476/annotation1853280/range1853280/1
type: Range
persistent identity: range1853280
display id: range1853280
version: 1
direction: direction/reverse
top level: Pr+YFP-
start: 58
end: 69
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1853297
display id: annotation1853297
version: 1
top level: Pr+YFP-
location: https://synbiohub.org/public/igem/BBa_S03476/annotation1853297/range1853297/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_S03476/annotation1853297/range1853297/1
type: Range
persistent identity: range1853297
display id: range1853297
version: 1
direction: direction/forward
top level: Pr+YFP-
start: 895
end: 935
orientation: inline

BBa_E0030
type: SequenceAnnotation
persistent identity: annotation1853282
display id: annotation1853282
version: 1
top level: Pr+YFP-
location: https://synbiohub.org/public/igem/BBa_S03476/annotation1853282/range1853282/1
component: BBa_E0030

https://synbiohub.org/public/igem/BBa_S03476/annotation1853282/range1853282/1
type: Range
persistent identity: range1853282
display id: range1853282
version: 1
direction: direction/reverse
top level: Pr+YFP-
start: 76
end: 798
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1853287
display id: annotation1853287
version: 1
top level: Pr+YFP-
location: https://synbiohub.org/public/igem/BBa_S03476/annotation1853287/range1853287/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_S03476/annotation1853287/range1853287/1
type: Range
persistent identity: range1853287
display id: range1853287
version: 1
direction: direction/forward
top level: Pr+YFP-
start: 807
end: 886
orientation: inline

Pr + GFP
type: ComponentDefinition
persistent identity: BBa S03335
display id: BBa_S03335
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Pr with GFP as a reporter
creator: ETH Zurich iGEM Team 2005
created: 2005-09-20T11:00:00Z
modified: 2015-05-08T01:14:21Z
discontinued: false
dominant: true
experience: experience/None
group u list: _34_
m user id: 0
owner id: 398
owning group id: 34
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Pr promoter of the lambda-system combined with GFP as a quantification and debugging device for the INPUT-module of the ETHZ05 iGEM team.
mutable notes: For more details see Sensing Device and Event Processing Device documentation.
mutable provenance: Registry 7.05
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_B0010
component: BBa_R0051
component: BBa_B0012
component: BBa_B0034
component: BBa_E0040
sequence annotation: BBa_B0010
sequence annotation: BBa_R0051
sequence annotation: BBa_E0040
sequence annotation: BBa_B0012
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_S03335_sequence/1

BBa_B0010
type: Component
persistent identity: component1700226
display id: component1700226
version: 1
top level: Pr + GFP
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_R0051
type: Component
persistent identity: component1700211
display id: component1700211
version: 1
top level: Pr + GFP
access: public
definition: https://synbiohub.org/public/igem/BBa_R0051/1

BBa_B0012
type: Component
persistent identity: component1700236
display id: component1700236
version: 1
top level: Pr + GFP
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0034
type: Component
persistent identity: component1700219
display id: component1700219
version: 1
top level: Pr + GFP
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_E0040
type: Component
persistent identity: component1700221
display id: component1700221
version: 1
top level: Pr + GFP
access: public
definition: https://synbiohub.org/public/igem/BBa_E0040/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1700226
display id: annotation1700226
version: 1
top level: Pr + GFP
location: https://synbiohub.org/public/igem/BBa_S03335/annotation1700226/range1700226/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_S03335/annotation1700226/range1700226/1
type: Range
persistent identity: range1700226
display id: range1700226
version: 1
direction: direction/forward
top level: Pr + GFP
start: 804
end: 883
orientation: inline

BBa_R0051
type: SequenceAnnotation
persistent identity: annotation1700211
display id: annotation1700211
version: 1
top level: Pr + GFP
location: https://synbiohub.org/public/igem/BBa_S03335/annotation1700211/range1700211/1
component: BBa_R0051

https://synbiohub.org/public/igem/BBa_S03335/annotation1700211/range1700211/1
type: Range
persistent identity: range1700211
display id: range1700211
version: 1
direction: direction/forward
top level: Pr + GFP
start: 1
end: 49
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation1700221
display id: annotation1700221
version: 1
top level: Pr + GFP
location: https://synbiohub.org/public/igem/BBa_S03335/annotation1700221/range1700221/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_S03335/annotation1700221/range1700221/1
type: Range
persistent identity: range1700221
display id: range1700221
version: 1
direction: direction/forward
top level: Pr + GFP
start: 76
end: 795
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1700236
display id: annotation1700236
version: 1
top level: Pr + GFP
location: https://synbiohub.org/public/igem/BBa_S03335/annotation1700236/range1700236/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_S03335/annotation1700236/range1700236/1
type: Range
persistent identity: range1700236
display id: range1700236
version: 1
direction: direction/forward
top level: Pr + GFP
start: 892
end: 932
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1700219
display id: annotation1700219
version: 1
top level: Pr + GFP
location: https://synbiohub.org/public/igem/BBa_S03335/annotation1700219/range1700219/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_S03335/annotation1700219/range1700219/1
type: Range
persistent identity: range1700219
display id: range1700219
version: 1
direction: direction/forward
top level: Pr + GFP
start: 58
end: 69
orientation: inline

BBa_I763003
type: ComponentDefinition
persistent identity: BBa I763003
display id: BBa_I763003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: GFP coding device switched on by IPTG
creator: Alice Pasini
created: 2007-07-01T11:00:00Z
is replaced by: https://synbiohub.org/public/igem/BBa_J04430/1
modified: 2015-08-31T04:08:08Z
discontinued: true
dominant: false
experience: experience/None
group u list: _111_
m user id: 0
owner id: 2088
owning group id: 9
part status: Released HQ 2013
sample status: Discontinued
status: status/Deleted
bookmark: false
mutable description: GFP coding device composed by: -LacI Promoter switched on by IPTG (ROO10) -RBS with efficiency 1 (B0034) -GFP green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP(E0040) - Excitation max - 501nm Emission max - 511nm -Terminators (B0010, B0012)
mutable notes: Different biobricks are avaible: Plac+RBS, RBS+GFP, RBS+GFP+Term, GFP+Term
mutable provenance: Registry
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_B0010
component: BBa_B0034
component: BBa_R0010
component: BBa_E0040
component: BBa_B0012
sequence annotation: BBa_E0040
sequence annotation: BBa_B0010
sequence annotation: BBa_B0034
sequence annotation: BBa_B0012
sequence annotation: BBa_R0010
sequence: https://synbiohub.org/public/igem/BBa_I763003_sequence/1

BBa_B0010
type: Component
persistent identity: component1936081
display id: component1936081
version: 1
top level: BBa_I763003
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0034
type: Component
persistent identity: component1936078
display id: component1936078
version: 1
top level: BBa_I763003
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_R0010
type: Component
persistent identity: component1936070
display id: component1936070
version: 1
top level: BBa_I763003
access: public
definition: https://synbiohub.org/public/igem/BBa_R0010/1

BBa_E0040
type: Component
persistent identity: component1936080
display id: component1936080
version: 1
top level: BBa_I763003
access: public
definition: https://synbiohub.org/public/igem/BBa_E0040/1

BBa_B0012
type: Component
persistent identity: component1936083
display id: component1936083
version: 1
top level: BBa_I763003
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation1936080
display id: annotation1936080
version: 1
top level: BBa_I763003
location: https://synbiohub.org/public/igem/BBa_I763003/annotation1936080/range1936080/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_I763003/annotation1936080/range1936080/1
type: Range
persistent identity: range1936080
display id: range1936080
version: 1
direction: direction/reverse
top level: BBa_I763003
start: 227
end: 946
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1936081
display id: annotation1936081
version: 1
top level: BBa_I763003
location: https://synbiohub.org/public/igem/BBa_I763003/annotation1936081/range1936081/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I763003/annotation1936081/range1936081/1
type: Range
persistent identity: range1936081
display id: range1936081
version: 1
direction: direction/forward
top level: BBa_I763003
start: 955
end: 1034
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1936078
display id: annotation1936078
version: 1
top level: BBa_I763003
location: https://synbiohub.org/public/igem/BBa_I763003/annotation1936078/range1936078/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I763003/annotation1936078/range1936078/1
type: Range
persistent identity: range1936078
display id: range1936078
version: 1
direction: direction/reverse
top level: BBa_I763003
start: 209
end: 220
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1936083
display id: annotation1936083
version: 1
top level: BBa_I763003
location: https://synbiohub.org/public/igem/BBa_I763003/annotation1936083/range1936083/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I763003/annotation1936083/range1936083/1
type: Range
persistent identity: range1936083
display id: range1936083
version: 1
direction: direction/forward
top level: BBa_I763003
start: 1043
end: 1083
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation1936070
display id: annotation1936070
version: 1
top level: BBa_I763003
location: https://synbiohub.org/public/igem/BBa_I763003/annotation1936070/range1936070/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_I763003/annotation1936070/range1936070/1
type: Range
persistent identity: range1936070
display id: range1936070
version: 1
direction: direction/forward
top level: BBa_I763003
start: 1
end: 200
orientation: inline

BBa_K1067009
type: ComponentDefinition
persistent identity: BBa K1067009
display id: BBa_K1067009
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Periplasm transport visualization device
creator: Kristian Davidsen
created: 2013-09-27T11:00:00Z
modified: 2015-05-08T01:09:00Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1376_
m user id: 0
owner id: 16028
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable description: ggg
mutable notes: ggg
mutable provenance: ggg
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
sequence annotation: araO1L
sequence annotation: BBa_K1067007
sequence annotation: araO2
sequence annotation: -35
sequence annotation: -10
sequence annotation: araO1R
sequence annotation: rbs
sequence annotation: RFP mCherry
sequence annotation: GFP SF
sequence annotation: CRP binding site
sequence annotation: start
sequence annotation: stop
sequence annotation: araI1
sequence annotation: araI2
sequence annotation: TAT signal peptide
sequence annotation: araC
sequence annotation: BBa_K1067001
sequence annotation: Gap
sequence annotation: Tight pBAD promoter
sequence: https://synbiohub.org/public/igem/BBa_K1067009_sequence/1

araO1L
type: SequenceAnnotation
persistent identity: annotation2366505
display id: annotation2366505
version: 1
top level: BBa_K1067009
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1067009/annotation2366505/range2366505/1

https://synbiohub.org/public/igem/BBa_K1067009/annotation2366505/range2366505/1
type: Range
persistent identity: range2366505
display id: range2366505
version: 1
direction: direction/forward
top level: BBa_K1067009
start: 1045
end: 1061
orientation: inline

BBa_K1067007
type: SequenceAnnotation
persistent identity: annotation2366513
display id: annotation2366513
version: 1
top level: BBa_K1067009
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1067009/annotation2366513/range2366513/1

https://synbiohub.org/public/igem/BBa_K1067009/annotation2366513/range2366513/1
type: Range
persistent identity: range2366513
display id: range2366513
version: 1
direction: direction/reverse
top level: BBa_K1067009
start: 1
end: 1209
orientation: inline

araO2
type: SequenceAnnotation
persistent identity: annotation2366504
display id: annotation2366504
version: 1
top level: BBa_K1067009
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1067009/annotation2366504/range2366504/1

https://synbiohub.org/public/igem/BBa_K1067009/annotation2366504/range2366504/1
type: Range
persistent identity: range2366504
display id: range2366504
version: 1
direction: direction/forward
top level: BBa_K1067009
start: 908
end: 924
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2366511
display id: annotation2366511
version: 1
top level: BBa_K1067009
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1067009/annotation2366511/range2366511/1

https://synbiohub.org/public/igem/BBa_K1067009/annotation2366511/range2366511/1
type: Range
persistent identity: range2366511
display id: range2366511
version: 1
direction: direction/forward
top level: BBa_K1067009
start: 1153
end: 1158
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2366512
display id: annotation2366512
version: 1
top level: BBa_K1067009
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1067009/annotation2366512/range2366512/1

https://synbiohub.org/public/igem/BBa_K1067009/annotation2366512/range2366512/1
type: Range
persistent identity: range2366512
display id: range2366512
version: 1
direction: direction/forward
top level: BBa_K1067009
start: 1177
end: 1182
orientation: inline

araO1R
type: SequenceAnnotation
persistent identity: annotation2366506
display id: annotation2366506
version: 1
top level: BBa_K1067009
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1067009/annotation2366506/range2366506/1

https://synbiohub.org/public/igem/BBa_K1067009/annotation2366506/range2366506/1
type: Range
persistent identity: range2366506
display id: range2366506
version: 1
direction: direction/forward
top level: BBa_K1067009
start: 1066
end: 1082
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation2366519
display id: annotation2366519
version: 1
top level: BBa_K1067009
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1067009/annotation2366519/range2366519/1

https://synbiohub.org/public/igem/BBa_K1067009/annotation2366519/range2366519/1
type: Range
persistent identity: range2366519
display id: range2366519
version: 1
direction: direction/forward
top level: BBa_K1067009
start: 2079
end: 2083
orientation: inline

RFP mCherry
type: SequenceAnnotation
persistent identity: annotation2366521
display id: annotation2366521
version: 1
top level: BBa_K1067009
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1067009/annotation2366521/range2366521/1

https://synbiohub.org/public/igem/BBa_K1067009/annotation2366521/range2366521/1
type: Range
persistent identity: range2366521
display id: range2366521
version: 1
direction: direction/forward
top level: BBa_K1067009
start: 2091
end: 2801
orientation: inline

GFP SF
type: SequenceAnnotation
persistent identity: annotation2366516
display id: annotation2366516
version: 1
top level: BBa_K1067009
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1067009/annotation2366516/range2366516/1

https://synbiohub.org/public/igem/BBa_K1067009/annotation2366516/range2366516/1
type: Range
persistent identity: range2366516
display id: range2366516
version: 1
direction: direction/forward
top level: BBa_K1067009
start: 1357
end: 2070
orientation: inline

CRP binding site
type: SequenceAnnotation
persistent identity: annotation2366507
display id: annotation2366507
version: 1
top level: BBa_K1067009
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1067009/annotation2366507/range2366507/1

https://synbiohub.org/public/igem/BBa_K1067009/annotation2366507/range2366507/1
type: Range
persistent identity: range2366507
display id: range2366507
version: 1
direction: direction/forward
top level: BBa_K1067009
start: 1087
end: 1108
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2366520
display id: annotation2366520
version: 1
top level: BBa_K1067009
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1067009/annotation2366520/range2366520/1

https://synbiohub.org/public/igem/BBa_K1067009/annotation2366520/range2366520/1
type: Range
persistent identity: range2366520
display id: range2366520
version: 1
direction: direction/forward
top level: BBa_K1067009
start: 2091
end: 2093
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2366522
display id: annotation2366522
version: 1
top level: BBa_K1067009
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1067009/annotation2366522/range2366522/1

https://synbiohub.org/public/igem/BBa_K1067009/annotation2366522/range2366522/1
type: Range
persistent identity: range2366522
display id: range2366522
version: 1
direction: direction/forward
top level: BBa_K1067009
start: 2799
end: 2801
orientation: inline

araI1
type: SequenceAnnotation
persistent identity: annotation2366508
display id: annotation2366508
version: 1
top level: BBa_K1067009
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1067009/annotation2366508/range2366508/1

https://synbiohub.org/public/igem/BBa_K1067009/annotation2366508/range2366508/1
type: Range
persistent identity: range2366508
display id: range2366508
version: 1
direction: direction/forward
top level: BBa_K1067009
start: 1119
end: 1135
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2366515
display id: annotation2366515
version: 1
top level: BBa_K1067009
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1067009/annotation2366515/range2366515/1

https://synbiohub.org/public/igem/BBa_K1067009/annotation2366515/range2366515/1
type: Range
persistent identity: range2366515
display id: range2366515
version: 1
direction: direction/forward
top level: BBa_K1067009
start: 1240
end: 1242
orientation: inline

araI2
type: SequenceAnnotation
persistent identity: annotation2366509
display id: annotation2366509
version: 1
top level: BBa_K1067009
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1067009/annotation2366509/range2366509/1

https://synbiohub.org/public/igem/BBa_K1067009/annotation2366509/range2366509/1
type: Range
persistent identity: range2366509
display id: range2366509
version: 1
direction: direction/forward
top level: BBa_K1067009
start: 1140
end: 1157
orientation: inline

TAT signal peptide
type: SequenceAnnotation
persistent identity: annotation2366514
display id: annotation2366514
version: 1
top level: BBa_K1067009
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1067009/annotation2366514/range2366514/1

https://synbiohub.org/public/igem/BBa_K1067009/annotation2366514/range2366514/1
type: Range
persistent identity: range2366514
display id: range2366514
version: 1
direction: direction/forward
top level: BBa_K1067009
start: 1240
end: 1356
orientation: inline

araC
type: SequenceAnnotation
persistent identity: annotation2366503
display id: annotation2366503
version: 1
top level: BBa_K1067009
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1067009/annotation2366503/range2366503/1

https://synbiohub.org/public/igem/BBa_K1067009/annotation2366503/range2366503/1
type: Range
persistent identity: range2366503
display id: range2366503
version: 1
direction: direction/forward
top level: BBa_K1067009
start: 1
end: 880
orientation: inline

BBa_K1067001
type: SequenceAnnotation
persistent identity: annotation2366523
display id: annotation2366523
version: 1
top level: BBa_K1067009
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1067009/annotation2366523/range2366523/1

https://synbiohub.org/public/igem/BBa_K1067009/annotation2366523/range2366523/1
type: Range
persistent identity: range2366523
display id: range2366523
version: 1
direction: direction/reverse
top level: BBa_K1067009
start: 1218
end: 2801
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2366517
display id: annotation2366517
version: 1
top level: BBa_K1067009
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1067009/annotation2366517/range2366517/1

https://synbiohub.org/public/igem/BBa_K1067009/annotation2366517/range2366517/1
type: Range
persistent identity: range2366517
display id: range2366517
version: 1
direction: direction/forward
top level: BBa_K1067009
start: 2068
end: 2070
orientation: inline

Gap
type: SequenceAnnotation
persistent identity: annotation2366518
display id: annotation2366518
version: 1
top level: BBa_K1067009
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1067009/annotation2366518/range2366518/1

https://synbiohub.org/public/igem/BBa_K1067009/annotation2366518/range2366518/1
type: Range
persistent identity: range2366518
display id: range2366518
version: 1
direction: direction/forward
top level: BBa_K1067009
start: 2071
end: 2090
orientation: inline

Tight pBAD promoter
type: SequenceAnnotation
persistent identity: annotation2366510
display id: annotation2366510
version: 1
top level: BBa_K1067009
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1067009/annotation2366510/range2366510/1

https://synbiohub.org/public/igem/BBa_K1067009/annotation2366510/range2366510/1
type: Range
persistent identity: range2366510
display id: range2366510
version: 1
direction: direction/forward
top level: BBa_K1067009
start: 1153
end: 1189
orientation: inline

BBa_K1067008
type: ComponentDefinition
persistent identity: BBa K1067008
display id: BBa_K1067008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: mCherry downstream tight pBAD
creator: Kristian Davidsen
created: 2013-09-27T11:00:00Z
modified: 2015-05-08T01:09:00Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1376_
m user id: 0
owner id: 16028
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: ggg
mutable notes: ggg
mutable provenance: ggg
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
sequence annotation: Tight pBAD promoter
sequence annotation: araC
sequence annotation: CRP binding site
sequence annotation: BBa_K1067007
sequence annotation: araO1L
sequence annotation: rbs
sequence annotation: araI1
sequence annotation: araI2
sequence annotation: mCherry
sequence annotation: -10
sequence annotation: araO2
sequence annotation: -35
sequence annotation: araO1R
sequence: https://synbiohub.org/public/igem/BBa_K1067008_sequence/1

Tight pBAD promoter
type: SequenceAnnotation
persistent identity: annotation2366497
display id: annotation2366497
version: 1
top level: BBa_K1067008
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1067008/annotation2366497/range2366497/1

https://synbiohub.org/public/igem/BBa_K1067008/annotation2366497/range2366497/1
type: Range
persistent identity: range2366497
display id: range2366497
version: 1
direction: direction/forward
top level: BBa_K1067008
start: 1153
end: 1189
orientation: inline

araC
type: SequenceAnnotation
persistent identity: annotation2366490
display id: annotation2366490
version: 1
top level: BBa_K1067008
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1067008/annotation2366490/range2366490/1

https://synbiohub.org/public/igem/BBa_K1067008/annotation2366490/range2366490/1
type: Range
persistent identity: range2366490
display id: range2366490
version: 1
direction: direction/forward
top level: BBa_K1067008
start: 1
end: 880
orientation: inline

CRP binding site
type: SequenceAnnotation
persistent identity: annotation2366494
display id: annotation2366494
version: 1
top level: BBa_K1067008
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1067008/annotation2366494/range2366494/1

https://synbiohub.org/public/igem/BBa_K1067008/annotation2366494/range2366494/1
type: Range
persistent identity: range2366494
display id: range2366494
version: 1
direction: direction/forward
top level: BBa_K1067008
start: 1087
end: 1108
orientation: inline

BBa_K1067007
type: SequenceAnnotation
persistent identity: annotation2366500
display id: annotation2366500
version: 1
top level: BBa_K1067008
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1067008/annotation2366500/range2366500/1

https://synbiohub.org/public/igem/BBa_K1067008/annotation2366500/range2366500/1
type: Range
persistent identity: range2366500
display id: range2366500
version: 1
direction: direction/reverse
top level: BBa_K1067008
start: 1
end: 1209
orientation: inline

araO1L
type: SequenceAnnotation
persistent identity: annotation2366492
display id: annotation2366492
version: 1
top level: BBa_K1067008
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1067008/annotation2366492/range2366492/1

https://synbiohub.org/public/igem/BBa_K1067008/annotation2366492/range2366492/1
type: Range
persistent identity: range2366492
display id: range2366492
version: 1
direction: direction/forward
top level: BBa_K1067008
start: 1045
end: 1061
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation2366502
display id: annotation2366502
version: 1
top level: BBa_K1067008
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1067008/annotation2366502/range2366502/1

https://synbiohub.org/public/igem/BBa_K1067008/annotation2366502/range2366502/1
type: Range
persistent identity: range2366502
display id: range2366502
version: 1
direction: direction/forward
top level: BBa_K1067008
start: 1225
end: 1229
orientation: inline

araI1
type: SequenceAnnotation
persistent identity: annotation2366495
display id: annotation2366495
version: 1
top level: BBa_K1067008
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1067008/annotation2366495/range2366495/1

https://synbiohub.org/public/igem/BBa_K1067008/annotation2366495/range2366495/1
type: Range
persistent identity: range2366495
display id: range2366495
version: 1
direction: direction/forward
top level: BBa_K1067008
start: 1119
end: 1135
orientation: inline

araI2
type: SequenceAnnotation
persistent identity: annotation2366496
display id: annotation2366496
version: 1
top level: BBa_K1067008
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1067008/annotation2366496/range2366496/1

https://synbiohub.org/public/igem/BBa_K1067008/annotation2366496/range2366496/1
type: Range
persistent identity: range2366496
display id: range2366496
version: 1
direction: direction/forward
top level: BBa_K1067008
start: 1140
end: 1157
orientation: inline

mCherry
type: SequenceAnnotation
persistent identity: annotation2366501
display id: annotation2366501
version: 1
top level: BBa_K1067008
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1067008/annotation2366501/range2366501/1

https://synbiohub.org/public/igem/BBa_K1067008/annotation2366501/range2366501/1
type: Range
persistent identity: range2366501
display id: range2366501
version: 1
direction: direction/forward
top level: BBa_K1067008
start: 1237
end: 1947
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2366499
display id: annotation2366499
version: 1
top level: BBa_K1067008
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1067008/annotation2366499/range2366499/1

https://synbiohub.org/public/igem/BBa_K1067008/annotation2366499/range2366499/1
type: Range
persistent identity: range2366499
display id: range2366499
version: 1
direction: direction/forward
top level: BBa_K1067008
start: 1177
end: 1182
orientation: inline

araO2
type: SequenceAnnotation
persistent identity: annotation2366491
display id: annotation2366491
version: 1
top level: BBa_K1067008
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1067008/annotation2366491/range2366491/1

https://synbiohub.org/public/igem/BBa_K1067008/annotation2366491/range2366491/1
type: Range
persistent identity: range2366491
display id: range2366491
version: 1
direction: direction/forward
top level: BBa_K1067008
start: 908
end: 924
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2366498
display id: annotation2366498
version: 1
top level: BBa_K1067008
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1067008/annotation2366498/range2366498/1

https://synbiohub.org/public/igem/BBa_K1067008/annotation2366498/range2366498/1
type: Range
persistent identity: range2366498
display id: range2366498
version: 1
direction: direction/forward
top level: BBa_K1067008
start: 1153
end: 1158
orientation: inline

araO1R
type: SequenceAnnotation
persistent identity: annotation2366493
display id: annotation2366493
version: 1
top level: BBa_K1067008
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1067008/annotation2366493/range2366493/1

https://synbiohub.org/public/igem/BBa_K1067008/annotation2366493/range2366493/1
type: Range
persistent identity: range2366493
display id: range2366493
version: 1
direction: direction/forward
top level: BBa_K1067008
start: 1066
end: 1082
orientation: inline

BBa_I741104
type: ComponentDefinition
persistent identity: BBa I741104
display id: BBa_I741104
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter (I741020) and GFP (E0240)
creator: Lucien Weiss
created: 2007-06-21T11:00:00Z
modified: 2015-08-31T04:08:02Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _143_
m user id: 0
owner id: 1763
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_E0040
component: BBa_B0010
component: BBa_B0012
component: BBa_I741020
component: BBa_B0030
sequence annotation: BBa_E0040
sequence annotation: BBa_B0030
sequence annotation: BBa_B0012
sequence annotation: BBa_B0010
sequence annotation: BBa_I741020
sequence: https://synbiohub.org/public/igem/BBa_I741104_sequence/1

BBa_E0040
type: Component
persistent identity: component1935292
display id: component1935292
version: 1
top level: BBa_I741104
access: public
definition: https://synbiohub.org/public/igem/BBa_E0040/1

BBa_B0010
type: Component
persistent identity: component1935293
display id: component1935293
version: 1
top level: BBa_I741104
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
type: Component
persistent identity: component1935295
display id: component1935295
version: 1
top level: BBa_I741104
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_I741020
type: Component
persistent identity: component1935287
display id: component1935287
version: 1
top level: BBa_I741104
access: public
definition: https://synbiohub.org/public/igem/BBa_I741020/1

BBa_B0030
type: Component
persistent identity: component1935289
display id: component1935289
version: 1
top level: BBa_I741104
access: public
definition: https://synbiohub.org/public/igem/BBa_B0030/1

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation1935292
display id: annotation1935292
version: 1
top level: BBa_I741104
location: https://synbiohub.org/public/igem/BBa_I741104/annotation1935292/range1935292/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_I741104/annotation1935292/range1935292/1
type: Range
persistent identity: range1935292
display id: range1935292
version: 1
direction: direction/reverse
top level: BBa_I741104
start: 221
end: 940
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation1935289
display id: annotation1935289
version: 1
top level: BBa_I741104
location: https://synbiohub.org/public/igem/BBa_I741104/annotation1935289/range1935289/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_I741104/annotation1935289/range1935289/1
type: Range
persistent identity: range1935289
display id: range1935289
version: 1
direction: direction/forward
top level: BBa_I741104
start: 200
end: 214
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1935295
display id: annotation1935295
version: 1
top level: BBa_I741104
location: https://synbiohub.org/public/igem/BBa_I741104/annotation1935295/range1935295/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I741104/annotation1935295/range1935295/1
type: Range
persistent identity: range1935295
display id: range1935295
version: 1
direction: direction/forward
top level: BBa_I741104
start: 1037
end: 1077
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1935293
display id: annotation1935293
version: 1
top level: BBa_I741104
location: https://synbiohub.org/public/igem/BBa_I741104/annotation1935293/range1935293/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I741104/annotation1935293/range1935293/1
type: Range
persistent identity: range1935293
display id: range1935293
version: 1
direction: direction/forward
top level: BBa_I741104
start: 949
end: 1028
orientation: inline

BBa_I741020
type: SequenceAnnotation
persistent identity: annotation1935287
display id: annotation1935287
version: 1
top level: BBa_I741104
location: https://synbiohub.org/public/igem/BBa_I741104/annotation1935287/range1935287/1
component: BBa_I741020

https://synbiohub.org/public/igem/BBa_I741104/annotation1935287/range1935287/1
type: Range
persistent identity: range1935287
display id: range1935287
version: 1
direction: direction/reverse
top level: BBa_I741104
start: 1
end: 191
orientation: inline

BBa_C0470
type: ComponentDefinition
persistent identity: BBa C0470
display id: BBa_C0470
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Bgl Terminator with yfp reportor
creator: T. Senthil
created: 2005-10-28T11:00:00Z
modified: 2015-08-31T04:07:24Z
discontinued: false
dominant: false
group u list: _44_
m user id: 0
owner id: 439
owning group id: 44
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Two terminators in Bgl operon that are antiterminated by bglG with YFP reportor
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_C0450
component: BBa_E0430
sequence annotation: BBa_E0430
sequence annotation: BBa_C0450
sequence: https://synbiohub.org/public/igem/BBa_C0470_sequence/1

BBa_C0450
type: Component
persistent identity: component2221787
display id: component2221787
version: 1
top level: BBa_C0470
access: public
definition: https://synbiohub.org/public/igem/BBa_C0450/1

BBa_E0430
type: Component
persistent identity: component2221797
display id: component2221797
version: 1
top level: BBa_C0470
access: public
definition: https://synbiohub.org/public/igem/BBa_E0430/1

BBa_E0430
type: SequenceAnnotation
persistent identity: annotation2221797
display id: annotation2221797
version: 1
top level: BBa_C0470
location: https://synbiohub.org/public/igem/BBa_C0470/annotation2221797/range2221797/1
component: BBa_E0430

https://synbiohub.org/public/igem/BBa_C0470/annotation2221797/range2221797/1
type: Range
persistent identity: range2221797
display id: range2221797
version: 1
direction: direction/reverse
top level: BBa_C0470
start: 265
end: 1142
orientation: inline

BBa_C0450
type: SequenceAnnotation
persistent identity: annotation2221787
display id: annotation2221787
version: 1
top level: BBa_C0470
location: https://synbiohub.org/public/igem/BBa_C0470/annotation2221787/range2221787/1
component: BBa_C0450

https://synbiohub.org/public/igem/BBa_C0470/annotation2221787/range2221787/1
type: Range
persistent identity: range2221787
display id: range2221787
version: 1
direction: direction/reverse
top level: BBa_C0470
start: 1
end: 256
orientation: inline

BBa_J09266
type: ComponentDefinition
persistent identity: BBa J09266
display id: BBa_J09266
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: constitutive GFP (AAV+) device, medium RBS
creator: Jon Badalamenti
created: 2006-10-26T11:00:00Z
modified: 2015-08-31T04:08:21Z
discontinued: false
dominant: false
group u list: _72_40_
m user id: 0
owner id: 475
owning group id: 72
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: I14032.B0031.E0044.B0015
mutable notes: none.
mutable provenance: This part was constructed from parts available through the registry.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_B0010
component: BBa_I14032
component: BBa_E0044
component: BBa_B0012
component: BBa_B0031
sequence annotation: BBa_B0031
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_E0044
sequence annotation: BBa_I14032
sequence: https://synbiohub.org/public/igem/BBa_J09266_sequence/1

BBa_B0010
type: Component
persistent identity: component1907398
display id: component1907398
version: 1
top level: BBa_J09266
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_I14032
type: Component
persistent identity: component1907394
display id: component1907394
version: 1
top level: BBa_J09266
access: public
definition: https://synbiohub.org/public/igem/BBa_I14032/1

BBa_E0044
type: Component
persistent identity: component1907397
display id: component1907397
version: 1
top level: BBa_J09266
access: public
definition: https://synbiohub.org/public/igem/BBa_E0044/1

BBa_B0012
type: Component
persistent identity: component1907400
display id: component1907400
version: 1
top level: BBa_J09266
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0031
type: Component
persistent identity: component1907396
display id: component1907396
version: 1
top level: BBa_J09266
access: public
definition: https://synbiohub.org/public/igem/BBa_B0031/1

BBa_B0031
type: SequenceAnnotation
persistent identity: annotation1907396
display id: annotation1907396
version: 1
top level: BBa_J09266
location: https://synbiohub.org/public/igem/BBa_J09266/annotation1907396/range1907396/1
component: BBa_B0031

https://synbiohub.org/public/igem/BBa_J09266/annotation1907396/range1907396/1
type: Range
persistent identity: range1907396
display id: range1907396
version: 1
direction: direction/reverse
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BBa_B0010
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component: BBa_B0010

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BBa_B0012
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BBa_E0044
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BBa_I14032
type: SequenceAnnotation
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BBa_I741105
type: ComponentDefinition
persistent identity: BBa I741105
display id: BBa_I741105
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter (I741021) and GFP (E0240)
creator: Lucien Weiss
created: 2007-06-21T11:00:00Z
modified: 2015-08-31T04:08:02Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _143_
m user id: 0
owner id: 1763
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_B0012
component: BBa_I741021
component: BBa_E0040
component: BBa_B0030
component: BBa_B0010
sequence annotation: BBa_B0030
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_I741021
sequence annotation: BBa_E0040
sequence: https://synbiohub.org/public/igem/BBa_I741105_sequence/1

BBa_B0012
type: Component
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BBa_I741021
type: Component
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BBa_E0040
type: Component
persistent identity: component1935305
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definition: https://synbiohub.org/public/igem/BBa_E0040/1

BBa_B0030
type: Component
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display id: component1935302
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definition: https://synbiohub.org/public/igem/BBa_B0030/1

BBa_B0010
type: Component
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display id: component1935306
version: 1
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access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation1935302
display id: annotation1935302
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location: https://synbiohub.org/public/igem/BBa_I741105/annotation1935302/range1935302/1
component: BBa_B0030

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type: Range
persistent identity: range1935302
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BBa_B0010
type: SequenceAnnotation
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display id: annotation1935306
version: 1
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persistent identity: range1935306
display id: range1935306
version: 1
direction: direction/forward
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start: 845
end: 924
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BBa_B0012
type: SequenceAnnotation
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version: 1
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BBa_I741021
type: SequenceAnnotation
persistent identity: annotation1935300
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BBa_E0040
type: SequenceAnnotation
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display id: annotation1935305
version: 1
top level: BBa_I741105
location: https://synbiohub.org/public/igem/BBa_I741105/annotation1935305/range1935305/1
component: BBa_E0040

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type: Range
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BBa_K1399015
type: ComponentDefinition
persistent identity: BBa K1399015
display id: BBa_K1399015
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Plac-GFP(LVA)
creator: Anna Stikane
created: 2014-09-18T11:00:00Z
modified: 2015-05-08T01:10:16Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1777_
m user id: 0
owner id: 22477
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: Lactose/IPTG inducible promoter with GFP reporter tagged with AAV-ssrA degradation tag followed by terminator. The tagged GFP is actively degraded within cell, thus provides better temporal resolution of green fluorescence and promoter activity.
mutable notes: Part was assembled using BrickClip assembly (BBF RFC104) using other biobrick parts as templates. BrickClip assembly is a special case of more general Paperclip assembly method.[1]
mutable provenance: other biobricks
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_B0015
component: BBa_B0034
component: BBa_R0010
component: BBa_K1399004
sequence annotation: BBa_R0010
sequence annotation: BBa_B0034
sequence annotation: BBa_K1399004
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_K1399015_sequence/1

BBa_B0015
type: Component
persistent identity: component2384085
display id: component2384085
version: 1
top level: BBa_K1399015
access: public
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BBa_B0034
type: Component
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display id: component2384072
version: 1
top level: BBa_K1399015
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_R0010
type: Component
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display id: component2384064
version: 1
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access: public
definition: https://synbiohub.org/public/igem/BBa_R0010/1

BBa_K1399004
type: Component
persistent identity: component2384078
display id: component2384078
version: 1
top level: BBa_K1399015
access: public
definition: https://synbiohub.org/public/igem/BBa_K1399004/1

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation2384064
display id: annotation2384064
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top level: BBa_K1399015
location: https://synbiohub.org/public/igem/BBa_K1399015/annotation2384064/range2384064/1
component: BBa_R0010

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type: Range
persistent identity: range2384064
display id: range2384064
version: 1
direction: direction/forward
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start: 1
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BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2384072
display id: annotation2384072
version: 1
top level: BBa_K1399015
location: https://synbiohub.org/public/igem/BBa_K1399015/annotation2384072/range2384072/1
component: BBa_B0034

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type: Range
persistent identity: range2384072
display id: range2384072
version: 1
direction: direction/reverse
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end: 220
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BBa_K1399004
type: SequenceAnnotation
persistent identity: annotation2384078
display id: annotation2384078
version: 1
top level: BBa_K1399015
location: https://synbiohub.org/public/igem/BBa_K1399015/annotation2384078/range2384078/1
component: BBa_K1399004

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type: Range
persistent identity: range2384078
display id: range2384078
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direction: direction/reverse
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end: 979
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BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2384085
display id: annotation2384085
version: 1
top level: BBa_K1399015
location: https://synbiohub.org/public/igem/BBa_K1399015/annotation2384085/range2384085/1
component: BBa_B0015

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type: Range
persistent identity: range2384085
display id: range2384085
version: 1
direction: direction/reverse
top level: BBa_K1399015
start: 988
end: 1116
orientation: inline

BBa_J32029
type: ComponentDefinition
persistent identity: BBa J32029
display id: BBa_J32029
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Luminescent Reporter
creator: Austen Heinz
created: 2006-10-31T12:00:00Z
modified: 2015-08-31T04:08:46Z
discontinued: false
dominant: false
experience: experience/None
group u list: _50_
m user id: 0
owner id: 495
owning group id: 50
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: _
mutable notes: a
mutable provenance: a
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_B0010
component: BBa_R0011
component: BBa_B0012
component: BBa_J32007
component: BBa_B0034
sequence annotation: BBa_J32007
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_B0034
sequence annotation: BBa_R0011
sequence: https://synbiohub.org/public/igem/BBa_J32029_sequence/1

BBa_B0010
type: Component
persistent identity: component1909499
display id: component1909499
version: 1
top level: BBa_J32029
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_R0011
type: Component
persistent identity: component1909484
display id: component1909484
version: 1
top level: BBa_J32029
access: public
definition: https://synbiohub.org/public/igem/BBa_R0011/1

BBa_B0012
type: Component
persistent identity: component1909501
display id: component1909501
version: 1
top level: BBa_J32029
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_J32007
type: Component
persistent identity: component1909498
display id: component1909498
version: 1
top level: BBa_J32029
access: public
definition: https://synbiohub.org/public/igem/BBa_J32007/1

BBa_B0034
type: Component
persistent identity: component1909490
display id: component1909490
version: 1
top level: BBa_J32029
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_J32007
type: SequenceAnnotation
persistent identity: annotation1909498
display id: annotation1909498
version: 1
top level: BBa_J32029
location: https://synbiohub.org/public/igem/BBa_J32029/annotation1909498/range1909498/1
component: BBa_J32007

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direction: direction/reverse
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BBa_B0010
type: SequenceAnnotation
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display id: annotation1909499
version: 1
top level: BBa_J32029
location: https://synbiohub.org/public/igem/BBa_J32029/annotation1909499/range1909499/1
component: BBa_B0010

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type: Range
persistent identity: range1909499
display id: range1909499
version: 1
direction: direction/forward
top level: BBa_J32029
start: 5888
end: 5967
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1909501
display id: annotation1909501
version: 1
top level: BBa_J32029
location: https://synbiohub.org/public/igem/BBa_J32029/annotation1909501/range1909501/1
component: BBa_B0012

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type: Range
persistent identity: range1909501
display id: range1909501
version: 1
direction: direction/forward
top level: BBa_J32029
start: 5976
end: 6016
orientation: inline

BBa_B0034
type: SequenceAnnotation
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display id: annotation1909490
version: 1
top level: BBa_J32029
location: https://synbiohub.org/public/igem/BBa_J32029/annotation1909490/range1909490/1
component: BBa_B0034

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type: Range
persistent identity: range1909490
display id: range1909490
version: 1
direction: direction/reverse
top level: BBa_J32029
start: 64
end: 75
orientation: inline

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation1909484
display id: annotation1909484
version: 1
top level: BBa_J32029
location: https://synbiohub.org/public/igem/BBa_J32029/annotation1909484/range1909484/1
component: BBa_R0011

https://synbiohub.org/public/igem/BBa_J32029/annotation1909484/range1909484/1
type: Range
persistent identity: range1909484
display id: range1909484
version: 1
direction: direction/forward
top level: BBa_J32029
start: 1
end: 54
orientation: inline

BBa_K1399017
type: ComponentDefinition
persistent identity: BBa K1399017
display id: BBa_K1399017
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Plac-GFP(DAS+2)
creator: Anna Stikane
created: 2014-09-18T11:00:00Z
modified: 2015-05-08T01:10:16Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1777_
m user id: 0
owner id: 22477
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: Lactose/IPTG inducible promoter with GFP reporter tagged with NYADAS-ssrA degradation tag followed by terminator. The tagged GFP is actively degraded within cell, thus provides better temporal resolution of green fluorescence and promoter activity.
mutable notes: Part was assembled using BrickClip assembly (BBF RFC104) using other biobrick parts as templates. BrickClip assembly is a special case of more general Paperclip assembly method.[1]
mutable provenance: other biobricks
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_R0010
component: BBa_K1399007
component: BBa_B0034
component: BBa_B0015
sequence annotation: BBa_R0010
sequence annotation: BBa_K1399007
sequence annotation: BBa_B0015
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K1399017_sequence/1

BBa_R0010
type: Component
persistent identity: component2384175
display id: component2384175
version: 1
top level: BBa_K1399017
access: public
definition: https://synbiohub.org/public/igem/BBa_R0010/1

BBa_K1399007
type: Component
persistent identity: component2384189
display id: component2384189
version: 1
top level: BBa_K1399017
access: public
definition: https://synbiohub.org/public/igem/BBa_K1399007/1

BBa_B0034
type: Component
persistent identity: component2384183
display id: component2384183
version: 1
top level: BBa_K1399017
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0015
type: Component
persistent identity: component2384196
display id: component2384196
version: 1
top level: BBa_K1399017
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation2384175
display id: annotation2384175
version: 1
top level: BBa_K1399017
location: https://synbiohub.org/public/igem/BBa_K1399017/annotation2384175/range2384175/1
component: BBa_R0010

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type: Range
persistent identity: range2384175
display id: range2384175
version: 1
direction: direction/forward
top level: BBa_K1399017
start: 1
end: 200
orientation: inline

BBa_K1399007
type: SequenceAnnotation
persistent identity: annotation2384189
display id: annotation2384189
version: 1
top level: BBa_K1399017
location: https://synbiohub.org/public/igem/BBa_K1399017/annotation2384189/range2384189/1
component: BBa_K1399007

https://synbiohub.org/public/igem/BBa_K1399017/annotation2384189/range2384189/1
type: Range
persistent identity: range2384189
display id: range2384189
version: 1
direction: direction/reverse
top level: BBa_K1399017
start: 227
end: 985
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2384196
display id: annotation2384196
version: 1
top level: BBa_K1399017
location: https://synbiohub.org/public/igem/BBa_K1399017/annotation2384196/range2384196/1
component: BBa_B0015

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type: Range
persistent identity: range2384196
display id: range2384196
version: 1
direction: direction/reverse
top level: BBa_K1399017
start: 994
end: 1122
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2384183
display id: annotation2384183
version: 1
top level: BBa_K1399017
location: https://synbiohub.org/public/igem/BBa_K1399017/annotation2384183/range2384183/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1399017/annotation2384183/range2384183/1
type: Range
persistent identity: range2384183
display id: range2384183
version: 1
direction: direction/reverse
top level: BBa_K1399017
start: 209
end: 220
orientation: inline

BBa_K801100
type: ComponentDefinition
persistent identity: BBa K801100
display id: BBa_K801100
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RFP Coding Device - RFC10 and RFC25 compatible
creator: TU Munich
created: 2012-10-20T11:00:00Z
modified: 2016-10-14T06:54:47Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1057_
m user id: 31248
owner id: 14732
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: x
mutable notes: x
mutable provenance: x
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: Promoter (lacI regulated) BBa_R0010
sequence annotation: RBS (Elowitz 1999) BBa_B0034
sequence annotation: Red Fluorescent Protein from Discosoma striata BBa_E1010
sequence annotation: Barcode
sequence annotation: Double Terminator BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_K801100_sequence/1

Promoter (lacI regulated) BBa_R0010
type: SequenceAnnotation
persistent identity: annotation2213050
display id: annotation2213050
version: 1
top level: BBa_K801100
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K801100/annotation2213050/range2213050/1

https://synbiohub.org/public/igem/BBa_K801100/annotation2213050/range2213050/1
type: Range
persistent identity: range2213050
display id: range2213050
version: 1
direction: direction/forward
top level: BBa_K801100
start: 1
end: 200
orientation: inline

RBS (Elowitz 1999) BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2213051
display id: annotation2213051
version: 1
top level: BBa_K801100
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K801100/annotation2213051/range2213051/1

https://synbiohub.org/public/igem/BBa_K801100/annotation2213051/range2213051/1
type: Range
persistent identity: range2213051
display id: range2213051
version: 1
direction: direction/forward
top level: BBa_K801100
start: 209
end: 220
orientation: inline

Red Fluorescent Protein from Discosoma striata BBa_E1010
type: SequenceAnnotation
persistent identity: annotation2213052
display id: annotation2213052
version: 1
top level: BBa_K801100
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K801100/annotation2213052/range2213052/1

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type: Range
persistent identity: range2213052
display id: range2213052
version: 1
direction: direction/forward
top level: BBa_K801100
start: 227
end: 902
orientation: inline

Barcode
type: SequenceAnnotation
persistent identity: annotation2213053
display id: annotation2213053
version: 1
top level: BBa_K801100
role: SO:0000807
role: feature/barcode
location: https://synbiohub.org/public/igem/BBa_K801100/annotation2213053/range2213053/1

https://synbiohub.org/public/igem/BBa_K801100/annotation2213053/range2213053/1
type: Range
persistent identity: range2213053
display id: range2213053
version: 1
direction: direction/forward
top level: BBa_K801100
start: 908
end: 932
orientation: inline

Double Terminator BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2213054
display id: annotation2213054
version: 1
top level: BBa_K801100
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K801100/annotation2213054/range2213054/1

https://synbiohub.org/public/igem/BBa_K801100/annotation2213054/range2213054/1
type: Range
persistent identity: range2213054
display id: range2213054
version: 1
direction: direction/forward
top level: BBa_K801100
start: 941
end: 1069
orientation: inline

BBa_J50035
type: ComponentDefinition
persistent identity: BBa J50035
display id: BBa_J50035
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: GFP Regulated by lactose/IPTG
creator: John Schumm
created: 2006-06-26T11:00:00Z
modified: 2015-08-31T03:54:03Z
discontinued: false
dominant: true
group u list: _54_
m user id: 0
owner id: 672
owning group id: 54
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Pizza
mutable notes: Breadsticks
mutable provenance: Pepsi
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_R0010
component: BBa_E0040
component: BBa_B0012
component: BBa_B0030
component: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_E0040
sequence annotation: BBa_R0010
sequence annotation: BBa_B0030
sequence annotation: BBa_B0010
sequence: https://synbiohub.org/public/igem/BBa_J50035_sequence/1

BBa_R0010
type: Component
persistent identity: component1882756
display id: component1882756
version: 1
top level: BBa_J50035
access: public
definition: https://synbiohub.org/public/igem/BBa_R0010/1

BBa_E0040
type: Component
persistent identity: component1882766
display id: component1882766
version: 1
top level: BBa_J50035
access: public
definition: https://synbiohub.org/public/igem/BBa_E0040/1

BBa_B0012
type: Component
persistent identity: component1882769
display id: component1882769
version: 1
top level: BBa_J50035
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0030
type: Component
persistent identity: component1882764
display id: component1882764
version: 1
top level: BBa_J50035
access: public
definition: https://synbiohub.org/public/igem/BBa_B0030/1

BBa_B0010
type: Component
persistent identity: component1882767
display id: component1882767
version: 1
top level: BBa_J50035
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1882769
display id: annotation1882769
version: 1
top level: BBa_J50035
location: https://synbiohub.org/public/igem/BBa_J50035/annotation1882769/range1882769/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_J50035/annotation1882769/range1882769/1
type: Range
persistent identity: range1882769
display id: range1882769
version: 1
direction: direction/forward
top level: BBa_J50035
start: 1046
end: 1086
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation1882766
display id: annotation1882766
version: 1
top level: BBa_J50035
location: https://synbiohub.org/public/igem/BBa_J50035/annotation1882766/range1882766/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_J50035/annotation1882766/range1882766/1
type: Range
persistent identity: range1882766
display id: range1882766
version: 1
direction: direction/reverse
top level: BBa_J50035
start: 230
end: 949
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation1882756
display id: annotation1882756
version: 1
top level: BBa_J50035
location: https://synbiohub.org/public/igem/BBa_J50035/annotation1882756/range1882756/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_J50035/annotation1882756/range1882756/1
type: Range
persistent identity: range1882756
display id: range1882756
version: 1
direction: direction/forward
top level: BBa_J50035
start: 1
end: 200
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation1882764
display id: annotation1882764
version: 1
top level: BBa_J50035
location: https://synbiohub.org/public/igem/BBa_J50035/annotation1882764/range1882764/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_J50035/annotation1882764/range1882764/1
type: Range
persistent identity: range1882764
display id: range1882764
version: 1
direction: direction/forward
top level: BBa_J50035
start: 209
end: 223
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1882767
display id: annotation1882767
version: 1
top level: BBa_J50035
location: https://synbiohub.org/public/igem/BBa_J50035/annotation1882767/range1882767/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_J50035/annotation1882767/range1882767/1
type: Range
persistent identity: range1882767
display id: range1882767
version: 1
direction: direction/forward
top level: BBa_J50035
start: 958
end: 1037
orientation: inline

BBa_K1399014
type: ComponentDefinition
persistent identity: BBa K1399014
display id: BBa_K1399014
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Plac-GFP(AAV)
creator: Anna Stikane
created: 2014-09-18T11:00:00Z
modified: 2015-05-08T01:10:16Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1777_
m user id: 0
owner id: 22477
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: Lactose/IPTG inducible promoter with GFP reporter tagged with AAV-ssrA degradation tag followed by terminator. The tagged GFP is actively degraded within cell, thus provides better temporal resolution of green fluorescence and promoter activity.
mutable notes: Part was assembled using BrickClip assembly (BBF RFC104) using other biobrick parts as templates. BrickClip assembly is a special case of more general Paperclip assembly method.[1]
mutable provenance: other biobricks
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_B0015
component: BBa_R0010
component: BBa_B0034
component: BBa_K1399005
sequence annotation: BBa_B0015
sequence annotation: BBa_B0034
sequence annotation: BBa_R0010
sequence annotation: BBa_K1399005
sequence: https://synbiohub.org/public/igem/BBa_K1399014_sequence/1

BBa_B0015
type: Component
persistent identity: component2384062
display id: component2384062
version: 1
top level: BBa_K1399014
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_R0010
type: Component
persistent identity: component2384041
display id: component2384041
version: 1
top level: BBa_K1399014
access: public
definition: https://synbiohub.org/public/igem/BBa_R0010/1

BBa_B0034
type: Component
persistent identity: component2384049
display id: component2384049
version: 1
top level: BBa_K1399014
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K1399005
type: Component
persistent identity: component2384055
display id: component2384055
version: 1
top level: BBa_K1399014
access: public
definition: https://synbiohub.org/public/igem/BBa_K1399005/1

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2384062
display id: annotation2384062
version: 1
top level: BBa_K1399014
location: https://synbiohub.org/public/igem/BBa_K1399014/annotation2384062/range2384062/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K1399014/annotation2384062/range2384062/1
type: Range
persistent identity: range2384062
display id: range2384062
version: 1
direction: direction/reverse
top level: BBa_K1399014
start: 988
end: 1116
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2384049
display id: annotation2384049
version: 1
top level: BBa_K1399014
location: https://synbiohub.org/public/igem/BBa_K1399014/annotation2384049/range2384049/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1399014/annotation2384049/range2384049/1
type: Range
persistent identity: range2384049
display id: range2384049
version: 1
direction: direction/reverse
top level: BBa_K1399014
start: 209
end: 220
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation2384041
display id: annotation2384041
version: 1
top level: BBa_K1399014
location: https://synbiohub.org/public/igem/BBa_K1399014/annotation2384041/range2384041/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_K1399014/annotation2384041/range2384041/1
type: Range
persistent identity: range2384041
display id: range2384041
version: 1
direction: direction/forward
top level: BBa_K1399014
start: 1
end: 200
orientation: inline

BBa_K1399005
type: SequenceAnnotation
persistent identity: annotation2384055
display id: annotation2384055
version: 1
top level: BBa_K1399014
location: https://synbiohub.org/public/igem/BBa_K1399014/annotation2384055/range2384055/1
component: BBa_K1399005

https://synbiohub.org/public/igem/BBa_K1399014/annotation2384055/range2384055/1
type: Range
persistent identity: range2384055
display id: range2384055
version: 1
direction: direction/reverse
top level: BBa_K1399014
start: 227
end: 979
orientation: inline

BBa_J58103
type: ComponentDefinition
persistent identity: BBa J58103
display id: BBa_J58103
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Mutated promoter activated by OmpR-P with the reporter GFP
creator: Valencia iGEM 2006
created: 2006-10-25T11:00:00Z
modified: 2015-08-31T03:14:48Z
discontinued: false
dominant: false
experience: experience/None
group u list: _83_
m user id: 0
owner id: 1100
owning group id: 83
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: Many signalling cellular processes occur using a phosphorylation mechanism. In order to assemble a transmembranal pathway, that frees a phosphore, with a genetic network within the cell, we can use this promoter (R0083). That promoter is regulated positively (activated) by the transcription factor OmpR-P, and it contains only one operator in its sequence, whereas in the natural promoter (R0082) there are three. We put a well known protein downstream: GFP (E0040) with a RBS (B0034) and a Terminator (B0015). This composite part will be very useful to characterize many cellular biosensors, jointly with the part J58102.
mutable notes: none
mutable provenance: To make this composite part we assemby two parts from the Registry (a promoter (R0083) and a generator (I13504)).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_B0012
component: BBa_E0040
component: BBa_R0083
component: BBa_B0034
component: BBa_B0010
sequence annotation: BBa_B0010
sequence annotation: BBa_E0040
sequence annotation: BBa_R0083
sequence annotation: BBa_B0034
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_J58103_sequence/1

BBa_B0012
type: Component
persistent identity: component1905135
display id: component1905135
version: 1
top level: BBa_J58103
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_E0040
type: Component
persistent identity: component1905132
display id: component1905132
version: 1
top level: BBa_J58103
access: public
definition: https://synbiohub.org/public/igem/BBa_E0040/1

BBa_R0083
type: Component
persistent identity: component1905129
display id: component1905129
version: 1
top level: BBa_J58103
access: public
definition: https://synbiohub.org/public/igem/BBa_R0083/1

BBa_B0034
type: Component
persistent identity: component1905131
display id: component1905131
version: 1
top level: BBa_J58103
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0010
type: Component
persistent identity: component1905133
display id: component1905133
version: 1
top level: BBa_J58103
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1905133
display id: annotation1905133
version: 1
top level: BBa_J58103
location: https://synbiohub.org/public/igem/BBa_J58103/annotation1905133/range1905133/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_J58103/annotation1905133/range1905133/1
type: Range
persistent identity: range1905133
display id: range1905133
version: 1
direction: direction/forward
top level: BBa_J58103
start: 833
end: 912
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation1905132
display id: annotation1905132
version: 1
top level: BBa_J58103
location: https://synbiohub.org/public/igem/BBa_J58103/annotation1905132/range1905132/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_J58103/annotation1905132/range1905132/1
type: Range
persistent identity: range1905132
display id: range1905132
version: 1
direction: direction/reverse
top level: BBa_J58103
start: 105
end: 824
orientation: inline

BBa_R0083
type: SequenceAnnotation
persistent identity: annotation1905129
display id: annotation1905129
version: 1
top level: BBa_J58103
location: https://synbiohub.org/public/igem/BBa_J58103/annotation1905129/range1905129/1
component: BBa_R0083

https://synbiohub.org/public/igem/BBa_J58103/annotation1905129/range1905129/1
type: Range
persistent identity: range1905129
display id: range1905129
version: 1
direction: direction/reverse
top level: BBa_J58103
start: 1
end: 78
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1905131
display id: annotation1905131
version: 1
top level: BBa_J58103
location: https://synbiohub.org/public/igem/BBa_J58103/annotation1905131/range1905131/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_J58103/annotation1905131/range1905131/1
type: Range
persistent identity: range1905131
display id: range1905131
version: 1
direction: direction/reverse
top level: BBa_J58103
start: 87
end: 98
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1905135
display id: annotation1905135
version: 1
top level: BBa_J58103
location: https://synbiohub.org/public/igem/BBa_J58103/annotation1905135/range1905135/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_J58103/annotation1905135/range1905135/1
type: Range
persistent identity: range1905135
display id: range1905135
version: 1
direction: direction/forward
top level: BBa_J58103
start: 921
end: 961
orientation: inline

BBa_I13600
type: ComponentDefinition
persistent identity: BBa I13600
display id: BBa_I13600
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Tet with CFP reporter (without LVA tag)
creator: Christopher Batten, Victoria Chou, Kenneth Nesmith
created: 2004-07-15T11:00:00Z
modified: 2015-08-31T04:07:35Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _6_
m user id: 0
owner id: 101
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_B0012
component: BBa_B0034
component: BBa_R0040
component: BBa_E0020
component: BBa_B0010
sequence annotation: BBa_B0034
sequence annotation: BBa_E0020
sequence annotation: BBa_B0010
sequence annotation: BBa_R0040
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_I13600_sequence/1

BBa_B0012
type: Component
persistent identity: component947209
display id: component947209
version: 1
top level: BBa_I13600
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0034
type: Component
persistent identity: component947192
display id: component947192
version: 1
top level: BBa_I13600
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_R0040
type: Component
persistent identity: component947184
display id: component947184
version: 1
top level: BBa_I13600
access: public
definition: https://synbiohub.org/public/igem/BBa_R0040/1

BBa_E0020
type: Component
persistent identity: component947194
display id: component947194
version: 1
top level: BBa_I13600
access: public
definition: https://synbiohub.org/public/igem/BBa_E0020/1

BBa_B0010
type: Component
persistent identity: component947199
display id: component947199
version: 1
top level: BBa_I13600
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation947192
display id: annotation947192
version: 1
top level: BBa_I13600
location: https://synbiohub.org/public/igem/BBa_I13600/annotation947192/range947192/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I13600/annotation947192/range947192/1
type: Range
persistent identity: range947192
display id: range947192
version: 1
direction: direction/forward
top level: BBa_I13600
start: 63
end: 74
orientation: inline

BBa_E0020
type: SequenceAnnotation
persistent identity: annotation947194
display id: annotation947194
version: 1
top level: BBa_I13600
location: https://synbiohub.org/public/igem/BBa_I13600/annotation947194/range947194/1
component: BBa_E0020

https://synbiohub.org/public/igem/BBa_I13600/annotation947194/range947194/1
type: Range
persistent identity: range947194
display id: range947194
version: 1
direction: direction/forward
top level: BBa_I13600
start: 81
end: 803
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation947199
display id: annotation947199
version: 1
top level: BBa_I13600
location: https://synbiohub.org/public/igem/BBa_I13600/annotation947199/range947199/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I13600/annotation947199/range947199/1
type: Range
persistent identity: range947199
display id: range947199
version: 1
direction: direction/forward
top level: BBa_I13600
start: 812
end: 891
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation947184
display id: annotation947184
version: 1
top level: BBa_I13600
location: https://synbiohub.org/public/igem/BBa_I13600/annotation947184/range947184/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_I13600/annotation947184/range947184/1
type: Range
persistent identity: range947184
display id: range947184
version: 1
direction: direction/forward
top level: BBa_I13600
start: 1
end: 54
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation947209
display id: annotation947209
version: 1
top level: BBa_I13600
location: https://synbiohub.org/public/igem/BBa_I13600/annotation947209/range947209/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I13600/annotation947209/range947209/1
type: Range
persistent identity: range947209
display id: range947209
version: 1
direction: direction/forward
top level: BBa_I13600
start: 900
end: 940
orientation: inline

BBa_E70102
type: ComponentDefinition
persistent identity: BBa E70102
display id: BBa_E70102
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Reporter for dedicated translation
creator: Barry Canton
created: 2006-03-03T12:00:00Z
modified: 2015-08-31T04:07:27Z
discontinued: false
dominant: false
group u list: _11_6_
m user id: 0
owner id: 135
owning group id: 6
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_R0040
component: BBa_B0012
component: BBa_B0070
component: BBa_B0010
component: BBa_E0040
sequence annotation: BBa_B0070
sequence annotation: BBa_E0040
sequence annotation: BBa_B0012
sequence annotation: BBa_R0040
sequence annotation: BBa_B0010
sequence: https://synbiohub.org/public/igem/BBa_E70102_sequence/1

BBa_R0040
type: Component
persistent identity: component1815559
display id: component1815559
version: 1
top level: BBa_E70102
access: public
definition: https://synbiohub.org/public/igem/BBa_R0040/1

BBa_B0012
type: Component
persistent identity: component1815581
display id: component1815581
version: 1
top level: BBa_E70102
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0070
type: Component
persistent identity: component1815564
display id: component1815564
version: 1
top level: BBa_E70102
access: public
definition: https://synbiohub.org/public/igem/BBa_B0070/1

BBa_B0010
type: Component
persistent identity: component1815571
display id: component1815571
version: 1
top level: BBa_E70102
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_E0040
type: Component
persistent identity: component1815566
display id: component1815566
version: 1
top level: BBa_E70102
access: public
definition: https://synbiohub.org/public/igem/BBa_E0040/1

BBa_B0070
type: SequenceAnnotation
persistent identity: annotation1815564
display id: annotation1815564
version: 1
top level: BBa_E70102
location: https://synbiohub.org/public/igem/BBa_E70102/annotation1815564/range1815564/1
component: BBa_B0070

https://synbiohub.org/public/igem/BBa_E70102/annotation1815564/range1815564/1
type: Range
persistent identity: range1815564
display id: range1815564
version: 1
direction: direction/reverse
top level: BBa_E70102
start: 63
end: 78
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation1815566
display id: annotation1815566
version: 1
top level: BBa_E70102
location: https://synbiohub.org/public/igem/BBa_E70102/annotation1815566/range1815566/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_E70102/annotation1815566/range1815566/1
type: Range
persistent identity: range1815566
display id: range1815566
version: 1
direction: direction/reverse
top level: BBa_E70102
start: 85
end: 804
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1815581
display id: annotation1815581
version: 1
top level: BBa_E70102
location: https://synbiohub.org/public/igem/BBa_E70102/annotation1815581/range1815581/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_E70102/annotation1815581/range1815581/1
type: Range
persistent identity: range1815581
display id: range1815581
version: 1
direction: direction/forward
top level: BBa_E70102
start: 901
end: 941
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1815559
display id: annotation1815559
version: 1
top level: BBa_E70102
location: https://synbiohub.org/public/igem/BBa_E70102/annotation1815559/range1815559/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_E70102/annotation1815559/range1815559/1
type: Range
persistent identity: range1815559
display id: range1815559
version: 1
direction: direction/forward
top level: BBa_E70102
start: 1
end: 54
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1815571
display id: annotation1815571
version: 1
top level: BBa_E70102
location: https://synbiohub.org/public/igem/BBa_E70102/annotation1815571/range1815571/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_E70102/annotation1815571/range1815571/1
type: Range
persistent identity: range1815571
display id: range1815571
version: 1
direction: direction/forward
top level: BBa_E70102
start: 813
end: 892
orientation: inline

BBa_K283049
type: ComponentDefinition
persistent identity: BBa K283049
display id: BBa_K283049
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pLacI-rbs-tetR(LVA)
creator: Shi Lei, W Ling , Nelson Chu
created: 2009-10-12T11:00:00Z
modified: 2015-05-08T01:11:48Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _383_
m user id: 0
owner id: 5494
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: This is a GFP generator with a hypoxia inducible promoter
mutable notes: BBa_K283010
mutable provenance: The panas promoter is self-designed The rbs, GFP and the terminator are from the registry
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_C0040
component: BBa_R0010
component: BBa_B0034
sequence annotation: BBa_C0040
sequence annotation: BBa_B0034
sequence annotation: BBa_R0010
sequence: https://synbiohub.org/public/igem/BBa_K283049_sequence/1

BBa_C0040
type: Component
persistent identity: component2246659
display id: component2246659
version: 1
top level: BBa_K283049
access: public
definition: https://synbiohub.org/public/igem/BBa_C0040/1

BBa_R0010
type: Component
persistent identity: component2246647
display id: component2246647
version: 1
top level: BBa_K283049
access: public
definition: https://synbiohub.org/public/igem/BBa_R0010/1

BBa_B0034
type: Component
persistent identity: component2246655
display id: component2246655
version: 1
top level: BBa_K283049
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_C0040
type: SequenceAnnotation
persistent identity: annotation2246659
display id: annotation2246659
version: 1
top level: BBa_K283049
location: https://synbiohub.org/public/igem/BBa_K283049/annotation2246659/range2246659/1
component: BBa_C0040

https://synbiohub.org/public/igem/BBa_K283049/annotation2246659/range2246659/1
type: Range
persistent identity: range2246659
display id: range2246659
version: 1
direction: direction/reverse
top level: BBa_K283049
start: 227
end: 911
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2246655
display id: annotation2246655
version: 1
top level: BBa_K283049
location: https://synbiohub.org/public/igem/BBa_K283049/annotation2246655/range2246655/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K283049/annotation2246655/range2246655/1
type: Range
persistent identity: range2246655
display id: range2246655
version: 1
direction: direction/reverse
top level: BBa_K283049
start: 209
end: 220
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation2246647
display id: annotation2246647
version: 1
top level: BBa_K283049
location: https://synbiohub.org/public/igem/BBa_K283049/annotation2246647/range2246647/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_K283049/annotation2246647/range2246647/1
type: Range
persistent identity: range2246647
display id: range2246647
version: 1
direction: direction/forward
top level: BBa_K283049
start: 1
end: 200
orientation: inline

BBa_K283015
type: ComponentDefinition
persistent identity: BBa K283015
display id: BBa_K283015
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pTet-rbs-invasin(1-625aa)_RFP-terminators
creator: Nelson Chu, wei ling, Shi Lei
created: 2009-10-12T11:00:00Z
modified: 2015-05-08T01:11:47Z
discontinued: false
dominant: false
experience: experience/None
group u list: _383_
m user id: 0
owner id: 5494
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part is a generator for the fusion protein invisine(1-625)-RFP. The pTet promoter is constitutively on and can be repressed by TetR repressor protein.
mutable notes: BBa_K283015
mutable provenance: The fusion protein invisine(1-625)-RFP is self-designed The pTet promoter, rbs and the terminator are from the registry
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_B0034
component: BBa_K283040
component: BBa_R0040
sequence annotation: BBa_R0040
sequence annotation: BBa_K283040
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K283015_sequence/1

BBa_B0034
type: Component
persistent identity: component2052953
display id: component2052953
version: 1
top level: BBa_K283015
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K283040
type: Component
persistent identity: component2052961
display id: component2052961
version: 1
top level: BBa_K283015
access: public
definition: https://synbiohub.org/public/igem/BBa_K283040/1

BBa_R0040
type: Component
persistent identity: component2052947
display id: component2052947
version: 1
top level: BBa_K283015
access: public
definition: https://synbiohub.org/public/igem/BBa_R0040/1

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2052947
display id: annotation2052947
version: 1
top level: BBa_K283015
location: https://synbiohub.org/public/igem/BBa_K283015/annotation2052947/range2052947/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_K283015/annotation2052947/range2052947/1
type: Range
persistent identity: range2052947
display id: range2052947
version: 1
direction: direction/forward
top level: BBa_K283015
start: 1
end: 54
orientation: inline

BBa_K283040
type: SequenceAnnotation
persistent identity: annotation2052961
display id: annotation2052961
version: 1
top level: BBa_K283015
location: https://synbiohub.org/public/igem/BBa_K283015/annotation2052961/range2052961/1
component: BBa_K283040

https://synbiohub.org/public/igem/BBa_K283015/annotation2052961/range2052961/1
type: Range
persistent identity: range2052961
display id: range2052961
version: 1
direction: direction/reverse
top level: BBa_K283015
start: 81
end: 3177
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2052953
display id: annotation2052953
version: 1
top level: BBa_K283015
location: https://synbiohub.org/public/igem/BBa_K283015/annotation2052953/range2052953/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K283015/annotation2052953/range2052953/1
type: Range
persistent identity: range2052953
display id: range2052953
version: 1
direction: direction/reverse
top level: BBa_K283015
start: 63
end: 74
orientation: inline

Pr+YFP
type: ComponentDefinition
persistent identity: BBa S03474
display id: BBa_S03474
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Lambda-system Pr promoter with YFP reporter
creator: ETH Zurich iGEM Team 2005
created: 2006-05-01T11:00:00Z
modified: 2015-05-08T01:14:23Z
discontinued: false
dominant: false
group u list: _34_
m user id: 0
owner id: 398
owning group id: 34
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Testing Pr base level activity with specific reporter YFP.
mutable notes: For more details see Sensing Device and Event Processing Device in the ETH Team iGEM2005 WiKi documentation.
mutable provenance: Registry 7.05
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_B0012
component: BBa_R0051
component: BBa_E0032
component: BBa_B0010
component: BBa_B0034
sequence annotation: BBa_E0032
sequence annotation: BBa_B0034
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_R0051
sequence: https://synbiohub.org/public/igem/BBa_S03474_sequence/1

BBa_B0012
type: Component
persistent identity: component1853170
display id: component1853170
version: 1
top level: Pr+YFP
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_R0051
type: Component
persistent identity: component1853132
display id: component1853132
version: 1
top level: Pr+YFP
access: public
definition: https://synbiohub.org/public/igem/BBa_R0051/1

BBa_E0032
type: Component
persistent identity: component1853152
display id: component1853152
version: 1
top level: Pr+YFP
access: public
definition: https://synbiohub.org/public/igem/BBa_E0032/1

BBa_B0010
type: Component
persistent identity: component1853160
display id: component1853160
version: 1
top level: Pr+YFP
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0034
type: Component
persistent identity: component1853140
display id: component1853140
version: 1
top level: Pr+YFP
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_E0032
type: SequenceAnnotation
persistent identity: annotation1853152
display id: annotation1853152
version: 1
top level: Pr+YFP
location: https://synbiohub.org/public/igem/BBa_S03474/annotation1853152/range1853152/1
component: BBa_E0032

https://synbiohub.org/public/igem/BBa_S03474/annotation1853152/range1853152/1
type: Range
persistent identity: range1853152
display id: range1853152
version: 1
direction: direction/forward
top level: Pr+YFP
start: 76
end: 837
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1853140
display id: annotation1853140
version: 1
top level: Pr+YFP
location: https://synbiohub.org/public/igem/BBa_S03474/annotation1853140/range1853140/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_S03474/annotation1853140/range1853140/1
type: Range
persistent identity: range1853140
display id: range1853140
version: 1
direction: direction/reverse
top level: Pr+YFP
start: 58
end: 69
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1853160
display id: annotation1853160
version: 1
top level: Pr+YFP
location: https://synbiohub.org/public/igem/BBa_S03474/annotation1853160/range1853160/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_S03474/annotation1853160/range1853160/1
type: Range
persistent identity: range1853160
display id: range1853160
version: 1
direction: direction/forward
top level: Pr+YFP
start: 846
end: 925
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1853170
display id: annotation1853170
version: 1
top level: Pr+YFP
location: https://synbiohub.org/public/igem/BBa_S03474/annotation1853170/range1853170/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_S03474/annotation1853170/range1853170/1
type: Range
persistent identity: range1853170
display id: range1853170
version: 1
direction: direction/forward
top level: Pr+YFP
start: 934
end: 974
orientation: inline

BBa_R0051
type: SequenceAnnotation
persistent identity: annotation1853132
display id: annotation1853132
version: 1
top level: Pr+YFP
location: https://synbiohub.org/public/igem/BBa_S03474/annotation1853132/range1853132/1
component: BBa_R0051

https://synbiohub.org/public/igem/BBa_S03474/annotation1853132/range1853132/1
type: Range
persistent identity: range1853132
display id: range1853132
version: 1
direction: direction/forward
top level: Pr+YFP
start: 1
end: 49
orientation: inline

pLambda+RF
type: ComponentDefinition
persistent identity: BBa I763007
display id: BBa_I763007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: promoter lambda (cI regulated) with RFP reporter
creator: Alice Pasini
created: 2007-07-29T11:00:00Z
modified: 2015-08-31T04:08:08Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _111_
m user id: 0
owner id: 2088
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Promoter lambda - cI regulated - (R0051) with RFP (E1010) as a reporter. It can be used as a detector of the glucose metabolism.
mutable notes: Reporter
mutable provenance: Igem 2007 plates
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_R0051
component: BBa_I13507
sequence annotation: BBa_I13507
sequence annotation: BBa_R0051
sequence: https://synbiohub.org/public/igem/BBa_I763007_sequence/1

BBa_R0051
type: Component
persistent identity: component2252869
display id: component2252869
version: 1
top level: pLambda+RF
access: public
definition: https://synbiohub.org/public/igem/BBa_R0051/1

BBa_I13507
type: Component
persistent identity: component2252884
display id: component2252884
version: 1
top level: pLambda+RF
access: public
definition: https://synbiohub.org/public/igem/BBa_I13507/1

BBa_I13507
type: SequenceAnnotation
persistent identity: annotation2252884
display id: annotation2252884
version: 1
top level: pLambda+RF
location: https://synbiohub.org/public/igem/BBa_I763007/annotation2252884/range2252884/1
component: BBa_I13507

https://synbiohub.org/public/igem/BBa_I763007/annotation2252884/range2252884/1
type: Range
persistent identity: range2252884
display id: range2252884
version: 1
direction: direction/reverse
top level: pLambda+RF
start: 58
end: 918
orientation: inline

BBa_R0051
type: SequenceAnnotation
persistent identity: annotation2252869
display id: annotation2252869
version: 1
top level: pLambda+RF
location: https://synbiohub.org/public/igem/BBa_I763007/annotation2252869/range2252869/1
component: BBa_R0051

https://synbiohub.org/public/igem/BBa_I763007/annotation2252869/range2252869/1
type: Range
persistent identity: range2252869
display id: range2252869
version: 1
direction: direction/forward
top level: pLambda+RF
start: 1
end: 49
orientation: inline

BBa_J06005
type: ComponentDefinition
persistent identity: BBa J06005
display id: BBa_J06005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: YFP on LuxBox
creator: conniec
created: 2005-06-12T11:00:00Z
modified: 2015-08-31T04:08:16Z
discontinued: false
dominant: false
experience: experience/None
group u list: _20_
m user id: 0
owner id: 344
owning group id: 20
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Hybrid Lux promoter (repressed by cI) activates YFP reporter.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_E0032
component: BBa_R0065
component: BBa_B0034
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_R0065
sequence annotation: BBa_B0010
sequence annotation: BBa_B0034
sequence annotation: BBa_E0032
sequence: https://synbiohub.org/public/igem/BBa_J06005_sequence/1

BBa_E0032
type: Component
persistent identity: component1549774
display id: component1549774
version: 1
top level: BBa_J06005
access: public
definition: https://synbiohub.org/public/igem/BBa_E0032/1

BBa_R0065
type: Component
persistent identity: component1549750
display id: component1549750
version: 1
top level: BBa_J06005
access: public
definition: https://synbiohub.org/public/igem/BBa_R0065/1

BBa_B0034
type: Component
persistent identity: component1549762
display id: component1549762
version: 1
top level: BBa_J06005
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0012
type: Component
persistent identity: component1549792
display id: component1549792
version: 1
top level: BBa_J06005
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0010
type: Component
persistent identity: component1549782
display id: component1549782
version: 1
top level: BBa_J06005
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1549792
display id: annotation1549792
version: 1
top level: BBa_J06005
location: https://synbiohub.org/public/igem/BBa_J06005/annotation1549792/range1549792/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_J06005/annotation1549792/range1549792/1
type: Range
persistent identity: range1549792
display id: range1549792
version: 1
direction: direction/forward
top level: BBa_J06005
start: 982
end: 1022
orientation: inline

BBa_R0065
type: SequenceAnnotation
persistent identity: annotation1549750
display id: annotation1549750
version: 1
top level: BBa_J06005
location: https://synbiohub.org/public/igem/BBa_J06005/annotation1549750/range1549750/1
component: BBa_R0065

https://synbiohub.org/public/igem/BBa_J06005/annotation1549750/range1549750/1
type: Range
persistent identity: range1549750
display id: range1549750
version: 1
direction: direction/forward
top level: BBa_J06005
start: 1
end: 97
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1549782
display id: annotation1549782
version: 1
top level: BBa_J06005
location: https://synbiohub.org/public/igem/BBa_J06005/annotation1549782/range1549782/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_J06005/annotation1549782/range1549782/1
type: Range
persistent identity: range1549782
display id: range1549782
version: 1
direction: direction/forward
top level: BBa_J06005
start: 894
end: 973
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1549762
display id: annotation1549762
version: 1
top level: BBa_J06005
location: https://synbiohub.org/public/igem/BBa_J06005/annotation1549762/range1549762/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_J06005/annotation1549762/range1549762/1
type: Range
persistent identity: range1549762
display id: range1549762
version: 1
direction: direction/forward
top level: BBa_J06005
start: 106
end: 117
orientation: inline

BBa_E0032
type: SequenceAnnotation
persistent identity: annotation1549774
display id: annotation1549774
version: 1
top level: BBa_J06005
location: https://synbiohub.org/public/igem/BBa_J06005/annotation1549774/range1549774/1
component: BBa_E0032

https://synbiohub.org/public/igem/BBa_J06005/annotation1549774/range1549774/1
type: Range
persistent identity: range1549774
display id: range1549774
version: 1
direction: direction/forward
top level: BBa_J06005
start: 124
end: 885
orientation: inline

BBa_J45997
type: ComponentDefinition
persistent identity: BBa J45997
display id: BBa_J45997
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Stationary phase dependent GFP generator
creator: Stephen Payne
created: 2007-01-24T12:00:00Z
modified: 2015-08-31T04:08:50Z
discontinued: false
dominant: false
experience: experience/Fails
group u list: _84_
m user id: 0
owner id: 642
owning group id: 84
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part produces GFP in stationary phase via a truncated version of the osmY promoter (J45993).
mutable notes: No
mutable provenance: Composite part
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0012
component: BBa_E0040
component: BBa_J45993
component: BBa_B0010
component: BBa_B0030
sequence annotation: BBa_B0030
sequence annotation: BBa_B0010
sequence annotation: BBa_E0040
sequence annotation: BBa_B0012
sequence annotation: BBa_J45993
sequence: https://synbiohub.org/public/igem/BBa_J45997_sequence/1

BBa_B0012
type: Component
persistent identity: component1910666
display id: component1910666
version: 1
top level: BBa_J45997
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_E0040
type: Component
persistent identity: component1910663
display id: component1910663
version: 1
top level: BBa_J45997
access: public
definition: https://synbiohub.org/public/igem/BBa_E0040/1

BBa_J45993
type: Component
persistent identity: component1910659
display id: component1910659
version: 1
top level: BBa_J45997
access: public
definition: https://synbiohub.org/public/igem/BBa_J45993/1

BBa_B0010
type: Component
persistent identity: component1910664
display id: component1910664
version: 1
top level: BBa_J45997
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0030
type: Component
persistent identity: component1910661
display id: component1910661
version: 1
top level: BBa_J45997
access: public
definition: https://synbiohub.org/public/igem/BBa_B0030/1

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation1910661
display id: annotation1910661
version: 1
top level: BBa_J45997
location: https://synbiohub.org/public/igem/BBa_J45997/annotation1910661/range1910661/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_J45997/annotation1910661/range1910661/1
type: Range
persistent identity: range1910661
display id: range1910661
version: 1
direction: direction/forward
top level: BBa_J45997
start: 66
end: 80
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1910664
display id: annotation1910664
version: 1
top level: BBa_J45997
location: https://synbiohub.org/public/igem/BBa_J45997/annotation1910664/range1910664/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_J45997/annotation1910664/range1910664/1
type: Range
persistent identity: range1910664
display id: range1910664
version: 1
direction: direction/forward
top level: BBa_J45997
start: 815
end: 894
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation1910663
display id: annotation1910663
version: 1
top level: BBa_J45997
location: https://synbiohub.org/public/igem/BBa_J45997/annotation1910663/range1910663/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_J45997/annotation1910663/range1910663/1
type: Range
persistent identity: range1910663
display id: range1910663
version: 1
direction: direction/reverse
top level: BBa_J45997
start: 87
end: 806
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1910666
display id: annotation1910666
version: 1
top level: BBa_J45997
location: https://synbiohub.org/public/igem/BBa_J45997/annotation1910666/range1910666/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_J45997/annotation1910666/range1910666/1
type: Range
persistent identity: range1910666
display id: range1910666
version: 1
direction: direction/forward
top level: BBa_J45997
start: 903
end: 943
orientation: inline

BBa_J45993
type: SequenceAnnotation
persistent identity: annotation1910659
display id: annotation1910659
version: 1
top level: BBa_J45997
location: https://synbiohub.org/public/igem/BBa_J45997/annotation1910659/range1910659/1
component: BBa_J45993

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type: Range
persistent identity: range1910659
display id: range1910659
version: 1
direction: direction/reverse
top level: BBa_J45997
start: 1
end: 57
orientation: inline

BBa_I13601
type: ComponentDefinition
persistent identity: BBa I13601
display id: BBa_I13601
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Lac operator with CFP reporter (without LVA tag) [R/Lc-]
creator: Christopher Batten, Victoria Chou, Kenneth Nesmith
created: 2004-07-15T11:00:00Z
modified: 2015-08-31T04:07:36Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _6_
m user id: 0
owner id: 101
owning group id: 7
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_R0011
component: BBa_E0420
sequence annotation: BBa_R0011
sequence annotation: BBa_E0420
sequence: https://synbiohub.org/public/igem/BBa_I13601_sequence/1

BBa_R0011
type: Component
persistent identity: component2221463
display id: component2221463
version: 1
top level: BBa_I13601
access: public
definition: https://synbiohub.org/public/igem/BBa_R0011/1

BBa_E0420
type: Component
persistent identity: component2221477
display id: component2221477
version: 1
top level: BBa_I13601
access: public
definition: https://synbiohub.org/public/igem/BBa_E0420/1

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation2221463
display id: annotation2221463
version: 1
top level: BBa_I13601
location: https://synbiohub.org/public/igem/BBa_I13601/annotation2221463/range2221463/1
component: BBa_R0011

https://synbiohub.org/public/igem/BBa_I13601/annotation2221463/range2221463/1
type: Range
persistent identity: range2221463
display id: range2221463
version: 1
direction: direction/forward
top level: BBa_I13601
start: 1
end: 54
orientation: inline

BBa_E0420
type: SequenceAnnotation
persistent identity: annotation2221477
display id: annotation2221477
version: 1
top level: BBa_I13601
location: https://synbiohub.org/public/igem/BBa_I13601/annotation2221477/range2221477/1
component: BBa_E0420

https://synbiohub.org/public/igem/BBa_I13601/annotation2221477/range2221477/1
type: Range
persistent identity: range2221477
display id: range2221477
version: 1
direction: direction/reverse
top level: BBa_I13601
start: 64
end: 941
orientation: inline

BBa_K1399011
type: ComponentDefinition
persistent identity: BBa K1399011
display id: BBa_K1399011
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Plac-RFP(LVA)
creator: Anna Stikane
created: 2014-09-18T11:00:00Z
modified: 2015-05-08T01:10:16Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1777_
m user id: 0
owner id: 22477
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: Lactose/IPTG inducible promoter with RBS, RFP reporter tagged with LVA-ssrA degradation tag and terminator. The tagged RFP is actively degraded within cell, thus provides better temporal resolution of red fluorescence and promoter activity. This part can be considered as improved version of BBa_J04451 as it does not contain StuI restriction site coding for additional 'RP' residues between RFP and LVA tag.
mutable notes: Part was assembled using BrickClip assembly (BBF RFC 104) using other biobrick parts as templates. BrickClip assembly is a special case of more general Paperclip assembly method.[1]
mutable provenance: other biobricks
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: LacI binding site
sequence annotation: LVA-ssrA tag
sequence annotation: -35
sequence annotation: BBa_B0034
sequence annotation: CAP binding site
sequence annotation: BBa_B0015
sequence annotation: start
sequence annotation: stem_loop
sequence annotation: conserved
sequence annotation: stop
sequence annotation: BBa_R0010
sequence annotation: BBa_K1399001
sequence annotation: BBa_E1010 w/o stop
sequence annotation: end of LacI coding region (inactive)
sequence annotation: T7 TE
sequence annotation: BBa_B0010
sequence annotation: Barcode of BBa_E1010
sequence annotation: -10
sequence annotation: BBa_B0012
sequence annotation: polya
sequence: https://synbiohub.org/public/igem/BBa_K1399011_sequence/1

LacI binding site
type: SequenceAnnotation
persistent identity: annotation2386294
display id: annotation2386294
version: 1
top level: BBa_K1399011
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1399011/annotation2386294/range2386294/1

https://synbiohub.org/public/igem/BBa_K1399011/annotation2386294/range2386294/1
type: Range
persistent identity: range2386294
display id: range2386294
version: 1
direction: direction/forward
top level: BBa_K1399011
start: 166
end: 200
orientation: inline

LVA-ssrA tag
type: SequenceAnnotation
persistent identity: annotation2386301
display id: annotation2386301
version: 1
top level: BBa_K1399011
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1399011/annotation2386301/range2386301/1

https://synbiohub.org/public/igem/BBa_K1399011/annotation2386301/range2386301/1
type: Range
persistent identity: range2386301
display id: range2386301
version: 1
direction: direction/forward
top level: BBa_K1399011
start: 902
end: 934
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2386292
display id: annotation2386292
version: 1
top level: BBa_K1399011
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1399011/annotation2386292/range2386292/1

https://synbiohub.org/public/igem/BBa_K1399011/annotation2386292/range2386292/1
type: Range
persistent identity: range2386292
display id: range2386292
version: 1
direction: direction/forward
top level: BBa_K1399011
start: 137
end: 142
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2386296
display id: annotation2386296
version: 1
top level: BBa_K1399011
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1399011/annotation2386296/range2386296/1

https://synbiohub.org/public/igem/BBa_K1399011/annotation2386296/range2386296/1
type: Range
persistent identity: range2386296
display id: range2386296
version: 1
direction: direction/reverse
top level: BBa_K1399011
start: 209
end: 220
orientation: inline

CAP binding site
type: SequenceAnnotation
persistent identity: annotation2386291
display id: annotation2386291
version: 1
top level: BBa_K1399011
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1399011/annotation2386291/range2386291/1

https://synbiohub.org/public/igem/BBa_K1399011/annotation2386291/range2386291/1
type: Range
persistent identity: range2386291
display id: range2386291
version: 1
direction: direction/forward
top level: BBa_K1399011
start: 89
end: 126
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2386305
display id: annotation2386305
version: 1
top level: BBa_K1399011
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1399011/annotation2386305/range2386305/1

https://synbiohub.org/public/igem/BBa_K1399011/annotation2386305/range2386305/1
type: Range
persistent identity: range2386305
display id: range2386305
version: 1
direction: direction/reverse
top level: BBa_K1399011
start: 974
end: 1102
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2386298
display id: annotation2386298
version: 1
top level: BBa_K1399011
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1399011/annotation2386298/range2386298/1

https://synbiohub.org/public/igem/BBa_K1399011/annotation2386298/range2386298/1
type: Range
persistent identity: range2386298
display id: range2386298
version: 1
direction: direction/forward
top level: BBa_K1399011
start: 227
end: 229
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation2386307
display id: annotation2386307
version: 1
top level: BBa_K1399011
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1399011/annotation2386307/range2386307/1

https://synbiohub.org/public/igem/BBa_K1399011/annotation2386307/range2386307/1
type: Range
persistent identity: range2386307
display id: range2386307
version: 1
direction: direction/forward
top level: BBa_K1399011
start: 985
end: 1028
orientation: inline

conserved
type: SequenceAnnotation
persistent identity: annotation2386297
display id: annotation2386297
version: 1
top level: BBa_K1399011
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K1399011/annotation2386297/range2386297/1

https://synbiohub.org/public/igem/BBa_K1399011/annotation2386297/range2386297/1
type: Range
persistent identity: range2386297
display id: range2386297
version: 1
direction: direction/forward
top level: BBa_K1399011
start: 213
end: 216
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2386302
display id: annotation2386302
version: 1
top level: BBa_K1399011
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1399011/annotation2386302/range2386302/1

https://synbiohub.org/public/igem/BBa_K1399011/annotation2386302/range2386302/1
type: Range
persistent identity: range2386302
display id: range2386302
version: 1
direction: direction/forward
top level: BBa_K1399011
start: 935
end: 937
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation2386289
display id: annotation2386289
version: 1
top level: BBa_K1399011
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1399011/annotation2386289/range2386289/1

https://synbiohub.org/public/igem/BBa_K1399011/annotation2386289/range2386289/1
type: Range
persistent identity: range2386289
display id: range2386289
version: 1
direction: direction/forward
top level: BBa_K1399011
start: 1
end: 200
orientation: inline

BBa_K1399001
type: SequenceAnnotation
persistent identity: annotation2386299
display id: annotation2386299
version: 1
top level: BBa_K1399011
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1399011/annotation2386299/range2386299/1

https://synbiohub.org/public/igem/BBa_K1399011/annotation2386299/range2386299/1
type: Range
persistent identity: range2386299
display id: range2386299
version: 1
direction: direction/reverse
top level: BBa_K1399011
start: 227
end: 940
orientation: inline

BBa_E1010 w/o stop
type: SequenceAnnotation
persistent identity: annotation2386300
display id: annotation2386300
version: 1
top level: BBa_K1399011
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1399011/annotation2386300/range2386300/1

https://synbiohub.org/public/igem/BBa_K1399011/annotation2386300/range2386300/1
type: Range
persistent identity: range2386300
display id: range2386300
version: 1
direction: direction/forward
top level: BBa_K1399011
start: 230
end: 901
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2386295
display id: annotation2386295
version: 1
top level: BBa_K1399011
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1399011/annotation2386295/range2386295/1

https://synbiohub.org/public/igem/BBa_K1399011/annotation2386295/range2386295/1
type: Range
persistent identity: range2386295
display id: range2386295
version: 1
direction: direction/forward
top level: BBa_K1399011
start: 173
end: 173
orientation: inline

end of LacI coding region (inactive)
type: SequenceAnnotation
persistent identity: annotation2386290
display id: annotation2386290
version: 1
top level: BBa_K1399011
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1399011/annotation2386290/range2386290/1

https://synbiohub.org/public/igem/BBa_K1399011/annotation2386290/range2386290/1
type: Range
persistent identity: range2386290
display id: range2386290
version: 1
direction: direction/forward
top level: BBa_K1399011
start: 1
end: 88
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation2386309
display id: annotation2386309
version: 1
top level: BBa_K1399011
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1399011/annotation2386309/range2386309/1

https://synbiohub.org/public/igem/BBa_K1399011/annotation2386309/range2386309/1
type: Range
persistent identity: range2386309
display id: range2386309
version: 1
direction: direction/forward
top level: BBa_K1399011
start: 1069
end: 1088
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2386306
display id: annotation2386306
version: 1
top level: BBa_K1399011
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1399011/annotation2386306/range2386306/1

https://synbiohub.org/public/igem/BBa_K1399011/annotation2386306/range2386306/1
type: Range
persistent identity: range2386306
display id: range2386306
version: 1
direction: direction/forward
top level: BBa_K1399011
start: 974
end: 1053
orientation: inline

Barcode of BBa_E1010
type: SequenceAnnotation
persistent identity: annotation2386304
display id: annotation2386304
version: 1
top level: BBa_K1399011
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1399011/annotation2386304/range2386304/1

https://synbiohub.org/public/igem/BBa_K1399011/annotation2386304/range2386304/1
type: Range
persistent identity: range2386304
display id: range2386304
version: 1
direction: direction/forward
top level: BBa_K1399011
start: 941
end: 965
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2386293
display id: annotation2386293
version: 1
top level: BBa_K1399011
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1399011/annotation2386293/range2386293/1

https://synbiohub.org/public/igem/BBa_K1399011/annotation2386293/range2386293/1
type: Range
persistent identity: range2386293
display id: range2386293
version: 1
direction: direction/forward
top level: BBa_K1399011
start: 161
end: 166
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2386308
display id: annotation2386308
version: 1
top level: BBa_K1399011
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1399011/annotation2386308/range2386308/1

https://synbiohub.org/public/igem/BBa_K1399011/annotation2386308/range2386308/1
type: Range
persistent identity: range2386308
display id: range2386308
version: 1
direction: direction/forward
top level: BBa_K1399011
start: 1062
end: 1102
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation2386310
display id: annotation2386310
version: 1
top level: BBa_K1399011
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_K1399011/annotation2386310/range2386310/1

https://synbiohub.org/public/igem/BBa_K1399011/annotation2386310/range2386310/1
type: Range
persistent identity: range2386310
display id: range2386310
version: 1
direction: direction/forward
top level: BBa_K1399011
start: 1089
end: 1102
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2386303
display id: annotation2386303
version: 1
top level: BBa_K1399011
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1399011/annotation2386303/range2386303/1

https://synbiohub.org/public/igem/BBa_K1399011/annotation2386303/range2386303/1
type: Range
persistent identity: range2386303
display id: range2386303
version: 1
direction: direction/forward
top level: BBa_K1399011
start: 938
end: 940
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2386311
display id: annotation2386311
version: 1
top level: BBa_K1399011
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1399011/annotation2386311/range2386311/1

https://synbiohub.org/public/igem/BBa_K1399011/annotation2386311/range2386311/1
type: Range
persistent identity: range2386311
display id: range2386311
version: 1
direction: direction/forward
top level: BBa_K1399011
start: 1095
end: 1095
orientation: inline

BBa_I723124
type: ComponentDefinition
persistent identity: BBa I723124
display id: BBa_I723124
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Our test reporter, man!
creator: Maciej Trybilo
created: 2007-07-30T11:00:00Z
modified: 2015-08-31T04:07:54Z
discontinued: true
dominant: false
experience: experience/None
group u list: _126_
m user id: 0
owner id: 2129
owning group id: 9
sample status: Discontinued
status: status/Deleted
bookmark: false
mutable description: Long description
mutable notes: Design information in herrre
mutable provenance: Straight from my head
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_B0030
component: BBa_B0011
component: BBa_B0010
component: BBa_I723123
component: BBa_B0012
sequence annotation: BBa_B0012
sequence annotation: BBa_B0030
sequence annotation: BBa_I723123
sequence annotation: BBa_B0011
sequence annotation: BBa_B0010
sequence: https://synbiohub.org/public/igem/BBa_I723124_sequence/1

BBa_B0030
type: Component
persistent identity: component1940393
display id: component1940393
version: 1
top level: BBa_I723124
access: public
definition: https://synbiohub.org/public/igem/BBa_B0030/1

BBa_B0011
type: Component
persistent identity: component1940395
display id: component1940395
version: 1
top level: BBa_I723124
access: public
definition: https://synbiohub.org/public/igem/BBa_B0011/1

BBa_B0010
type: Component
persistent identity: component1940397
display id: component1940397
version: 1
top level: BBa_I723124
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_I723123
type: Component
persistent identity: component1940391
display id: component1940391
version: 1
top level: BBa_I723124
access: public
definition: https://synbiohub.org/public/igem/BBa_I723123/1

BBa_B0012
type: Component
persistent identity: component1940399
display id: component1940399
version: 1
top level: BBa_I723124
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1940399
display id: annotation1940399
version: 1
top level: BBa_I723124
location: https://synbiohub.org/public/igem/BBa_I723124/annotation1940399/range1940399/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I723124/annotation1940399/range1940399/1
type: Range
persistent identity: range1940399
display id: range1940399
version: 1
direction: direction/forward
top level: BBa_I723124
start: 223
end: 263
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation1940393
display id: annotation1940393
version: 1
top level: BBa_I723124
location: https://synbiohub.org/public/igem/BBa_I723124/annotation1940393/range1940393/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_I723124/annotation1940393/range1940393/1
type: Range
persistent identity: range1940393
display id: range1940393
version: 1
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end: 72
orientation: inline

BBa_I723123
type: SequenceAnnotation
persistent identity: annotation1940391
display id: annotation1940391
version: 1
top level: BBa_I723124
location: https://synbiohub.org/public/igem/BBa_I723124/annotation1940391/range1940391/1
component: BBa_I723123

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type: Range
persistent identity: range1940391
display id: range1940391
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BBa_B0011
type: SequenceAnnotation
persistent identity: annotation1940395
display id: annotation1940395
version: 1
top level: BBa_I723124
location: https://synbiohub.org/public/igem/BBa_I723124/annotation1940395/range1940395/1
component: BBa_B0011

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display id: range1940395
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end: 126
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1940397
display id: annotation1940397
version: 1
top level: BBa_I723124
location: https://synbiohub.org/public/igem/BBa_I723124/annotation1940397/range1940397/1
component: BBa_B0010

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end: 214
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BBa_J04431
type: ComponentDefinition
persistent identity: BBa J04431
display id: BBa_J04431
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: GFP Coding Device with promoter, RBS, GFP with LVA tag, and Terminator
creator: Kristen DeCelle
created: 2005-06-08T11:00:00Z
modified: 2015-08-31T04:08:14Z
discontinued: false
dominant: true
experience: experience/None
group u list: _16_
m user id: 0
owner id: 326
owning group id: 16
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: IPTG inducible promoter, RBS, GFP with LVA tag, and terminator.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_B0034
component: BBa_B0012
component: BBa_B0010
component: BBa_R0010
component: BBa_J04031
sequence annotation: BBa_B0010
sequence annotation: BBa_B0034
sequence annotation: BBa_J04031
sequence annotation: BBa_B0012
sequence annotation: BBa_R0010
sequence: https://synbiohub.org/public/igem/BBa_J04431_sequence/1

BBa_B0034
type: Component
persistent identity: component1508090
display id: component1508090
version: 1
top level: BBa_J04431
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0012
type: Component
persistent identity: component1508119
display id: component1508119
version: 1
top level: BBa_J04431
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0010
type: Component
persistent identity: component1508109
display id: component1508109
version: 1
top level: BBa_J04431
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_R0010
type: Component
persistent identity: component1508080
display id: component1508080
version: 1
top level: BBa_J04431
access: public
definition: https://synbiohub.org/public/igem/BBa_R0010/1

BBa_J04031
type: Component
persistent identity: component1508104
display id: component1508104
version: 1
top level: BBa_J04431
access: public
definition: https://synbiohub.org/public/igem/BBa_J04031/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1508109
display id: annotation1508109
version: 1
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location: https://synbiohub.org/public/igem/BBa_J04431/annotation1508109/range1508109/1
component: BBa_B0010

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display id: range1508109
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direction: direction/forward
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start: 994
end: 1073
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1508090
display id: annotation1508090
version: 1
top level: BBa_J04431
location: https://synbiohub.org/public/igem/BBa_J04431/annotation1508090/range1508090/1
component: BBa_B0034

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type: Range
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display id: range1508090
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start: 209
end: 220
orientation: inline

BBa_J04031
type: SequenceAnnotation
persistent identity: annotation1508104
display id: annotation1508104
version: 1
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location: https://synbiohub.org/public/igem/BBa_J04431/annotation1508104/range1508104/1
component: BBa_J04031

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display id: range1508104
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BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1508119
display id: annotation1508119
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location: https://synbiohub.org/public/igem/BBa_J04431/annotation1508119/range1508119/1
component: BBa_B0012

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type: Range
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display id: range1508119
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start: 1082
end: 1122
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BBa_R0010
type: SequenceAnnotation
persistent identity: annotation1508080
display id: annotation1508080
version: 1
top level: BBa_J04431
location: https://synbiohub.org/public/igem/BBa_J04431/annotation1508080/range1508080/1
component: BBa_R0010

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type: Range
persistent identity: range1508080
display id: range1508080
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Pr+CFP
type: ComponentDefinition
persistent identity: BBa S03464
display id: BBa_S03464
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Pr with CFP as a reporter
creator: Dominic Frutiger
created: 2006-05-01T11:00:00Z
modified: 2015-05-08T01:14:23Z
discontinued: false
dominant: false
group u list: _34_
m user id: 0
owner id: 398
owning group id: 34
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Pr promoter of the lambda-system combined with CFP as a quantification and debugging device for the INPUT-module of the ETHZ05 iGEM team.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_E0022
component: BBa_B0010
component: BBa_B0034
component: BBa_R0051
component: BBa_B0012
sequence annotation: BBa_B0010
sequence annotation: BBa_R0051
sequence annotation: BBa_B0034
sequence annotation: BBa_B0012
sequence annotation: BBa_E0022
sequence: https://synbiohub.org/public/igem/BBa_S03464_sequence/1

BBa_E0022
type: Component
persistent identity: component1852148
display id: component1852148
version: 1
top level: Pr+CFP
access: public
definition: https://synbiohub.org/public/igem/BBa_E0022/1

BBa_B0010
type: Component
persistent identity: component1852156
display id: component1852156
version: 1
top level: Pr+CFP
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0034
type: Component
persistent identity: component1852136
display id: component1852136
version: 1
top level: Pr+CFP
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_R0051
type: Component
persistent identity: component1852128
display id: component1852128
version: 1
top level: Pr+CFP
access: public
definition: https://synbiohub.org/public/igem/BBa_R0051/1

BBa_B0012
type: Component
persistent identity: component1852166
display id: component1852166
version: 1
top level: Pr+CFP
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1852156
display id: annotation1852156
version: 1
top level: Pr+CFP
location: https://synbiohub.org/public/igem/BBa_S03464/annotation1852156/range1852156/1
component: BBa_B0010

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type: Range
persistent identity: range1852156
display id: range1852156
version: 1
direction: direction/forward
top level: Pr+CFP
start: 846
end: 925
orientation: inline

BBa_R0051
type: SequenceAnnotation
persistent identity: annotation1852128
display id: annotation1852128
version: 1
top level: Pr+CFP
location: https://synbiohub.org/public/igem/BBa_S03464/annotation1852128/range1852128/1
component: BBa_R0051

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type: Range
persistent identity: range1852128
display id: range1852128
version: 1
direction: direction/forward
top level: Pr+CFP
start: 1
end: 49
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1852136
display id: annotation1852136
version: 1
top level: Pr+CFP
location: https://synbiohub.org/public/igem/BBa_S03464/annotation1852136/range1852136/1
component: BBa_B0034

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type: Range
persistent identity: range1852136
display id: range1852136
version: 1
direction: direction/reverse
top level: Pr+CFP
start: 58
end: 69
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1852166
display id: annotation1852166
version: 1
top level: Pr+CFP
location: https://synbiohub.org/public/igem/BBa_S03464/annotation1852166/range1852166/1
component: BBa_B0012

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type: Range
persistent identity: range1852166
display id: range1852166
version: 1
direction: direction/forward
top level: Pr+CFP
start: 934
end: 974
orientation: inline

BBa_E0022
type: SequenceAnnotation
persistent identity: annotation1852148
display id: annotation1852148
version: 1
top level: Pr+CFP
location: https://synbiohub.org/public/igem/BBa_S03464/annotation1852148/range1852148/1
component: BBa_E0022

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type: Range
persistent identity: range1852148
display id: range1852148
version: 1
direction: direction/forward
top level: Pr+CFP
start: 76
end: 837
orientation: inline

Little Bub
type: ComponentDefinition
persistent identity: BBa J06105
display id: BBa_J06105
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: YFP on LasBox
creator: kxjin
created: 2005-06-20T11:00:00Z
modified: 2015-08-31T04:08:16Z
discontinued: false
dominant: true
experience: experience/None
group u list: _20_
m user id: 0
owner id: 368
owning group id: 20
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Hybrid Las promoter (repressed by cI) activates YFP reporter.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_E0032
component: BBa_B0010
component: BBa_I14016
component: BBa_B0012
component: BBa_B0034
sequence annotation: BBa_I14016
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_B0034
sequence annotation: BBa_E0032
sequence: https://synbiohub.org/public/igem/BBa_J06105_sequence/1

BBa_E0032
type: Component
persistent identity: component1532054
display id: component1532054
version: 1
top level: Little Bub
access: public
definition: https://synbiohub.org/public/igem/BBa_E0032/1

BBa_B0010
type: Component
persistent identity: component1532062
display id: component1532062
version: 1
top level: Little Bub
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_I14016
type: Component
persistent identity: component1532037
display id: component1532037
version: 1
top level: Little Bub
access: public
definition: https://synbiohub.org/public/igem/BBa_I14016/1

BBa_B0012
type: Component
persistent identity: component1532072
display id: component1532072
version: 1
top level: Little Bub
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0034
type: Component
persistent identity: component1532042
display id: component1532042
version: 1
top level: Little Bub
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_I14016
type: SequenceAnnotation
persistent identity: annotation1532037
display id: annotation1532037
version: 1
top level: Little Bub
location: https://synbiohub.org/public/igem/BBa_J06105/annotation1532037/range1532037/1
component: BBa_I14016

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type: Range
persistent identity: range1532037
display id: range1532037
version: 1
direction: direction/forward
top level: Little Bub
start: 1
end: 168
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1532062
display id: annotation1532062
version: 1
top level: Little Bub
location: https://synbiohub.org/public/igem/BBa_J06105/annotation1532062/range1532062/1
component: BBa_B0010

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type: Range
persistent identity: range1532062
display id: range1532062
version: 1
direction: direction/forward
top level: Little Bub
start: 965
end: 1044
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1532072
display id: annotation1532072
version: 1
top level: Little Bub
location: https://synbiohub.org/public/igem/BBa_J06105/annotation1532072/range1532072/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_J06105/annotation1532072/range1532072/1
type: Range
persistent identity: range1532072
display id: range1532072
version: 1
direction: direction/forward
top level: Little Bub
start: 1053
end: 1093
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1532042
display id: annotation1532042
version: 1
top level: Little Bub
location: https://synbiohub.org/public/igem/BBa_J06105/annotation1532042/range1532042/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_J06105/annotation1532042/range1532042/1
type: Range
persistent identity: range1532042
display id: range1532042
version: 1
direction: direction/forward
top level: Little Bub
start: 177
end: 188
orientation: inline

BBa_E0032
type: SequenceAnnotation
persistent identity: annotation1532054
display id: annotation1532054
version: 1
top level: Little Bub
location: https://synbiohub.org/public/igem/BBa_J06105/annotation1532054/range1532054/1
component: BBa_E0032

https://synbiohub.org/public/igem/BBa_J06105/annotation1532054/range1532054/1
type: Range
persistent identity: range1532054
display id: range1532054
version: 1
direction: direction/forward
top level: Little Bub
start: 195
end: 956
orientation: inline

BBa_J13021
type: ComponentDefinition
persistent identity: BBa J13021
display id: BBa_J13021
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: 3OC6HSL+LuxR dependent EYFP generator
creator: jtabor
created: 2005-06-28T11:00:00Z
modified: 2015-08-31T04:08:29Z
discontinued: false
dominant: false
experience: experience/None
group u list: _37_
m user id: 0
owner id: 88
owning group id: 37
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_B0012
component: BBa_B0034
component: BBa_R0065
component: BBa_B0010
component: BBa_E0030
sequence annotation: BBa_B0012
sequence annotation: BBa_B0034
sequence annotation: BBa_E0030
sequence annotation: BBa_R0065
sequence annotation: BBa_B0010
sequence: https://synbiohub.org/public/igem/BBa_J13021_sequence/1

BBa_B0012
type: Component
persistent identity: component1556527
display id: component1556527
version: 1
top level: BBa_J13021
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0034
type: Component
persistent identity: component1556510
display id: component1556510
version: 1
top level: BBa_J13021
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_R0065
type: Component
persistent identity: component1556498
display id: component1556498
version: 1
top level: BBa_J13021
access: public
definition: https://synbiohub.org/public/igem/BBa_R0065/1

BBa_B0010
type: Component
persistent identity: component1556517
display id: component1556517
version: 1
top level: BBa_J13021
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_E0030
type: Component
persistent identity: component1556512
display id: component1556512
version: 1
top level: BBa_J13021
access: public
definition: https://synbiohub.org/public/igem/BBa_E0030/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1556527
display id: annotation1556527
version: 1
top level: BBa_J13021
location: https://synbiohub.org/public/igem/BBa_J13021/annotation1556527/range1556527/1
component: BBa_B0012

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type: Range
persistent identity: range1556527
display id: range1556527
version: 1
direction: direction/forward
top level: BBa_J13021
start: 943
end: 983
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1556510
display id: annotation1556510
version: 1
top level: BBa_J13021
location: https://synbiohub.org/public/igem/BBa_J13021/annotation1556510/range1556510/1
component: BBa_B0034

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type: Range
persistent identity: range1556510
display id: range1556510
version: 1
direction: direction/forward
top level: BBa_J13021
start: 106
end: 117
orientation: inline

BBa_E0030
type: SequenceAnnotation
persistent identity: annotation1556512
display id: annotation1556512
version: 1
top level: BBa_J13021
location: https://synbiohub.org/public/igem/BBa_J13021/annotation1556512/range1556512/1
component: BBa_E0030

https://synbiohub.org/public/igem/BBa_J13021/annotation1556512/range1556512/1
type: Range
persistent identity: range1556512
display id: range1556512
version: 1
direction: direction/forward
top level: BBa_J13021
start: 124
end: 846
orientation: inline

BBa_R0065
type: SequenceAnnotation
persistent identity: annotation1556498
display id: annotation1556498
version: 1
top level: BBa_J13021
location: https://synbiohub.org/public/igem/BBa_J13021/annotation1556498/range1556498/1
component: BBa_R0065

https://synbiohub.org/public/igem/BBa_J13021/annotation1556498/range1556498/1
type: Range
persistent identity: range1556498
display id: range1556498
version: 1
direction: direction/forward
top level: BBa_J13021
start: 1
end: 97
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1556517
display id: annotation1556517
version: 1
top level: BBa_J13021
location: https://synbiohub.org/public/igem/BBa_J13021/annotation1556517/range1556517/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_J13021/annotation1556517/range1556517/1
type: Range
persistent identity: range1556517
display id: range1556517
version: 1
direction: direction/forward
top level: BBa_J13021
start: 855
end: 934
orientation: inline

BBa_K1399010
type: ComponentDefinition
persistent identity: BBa K1399010
display id: BBa_K1399010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Plac-RFP(AAV)
creator: Anna Stikane
created: 2014-09-18T11:00:00Z
modified: 2015-05-08T01:10:16Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1777_
m user id: 0
owner id: 22477
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: Lactose/IPTG inducible promoter wuth RFP reporter tagged with AAV-ssrA degradation tag followed by terminator. The tagged RFP is actively degraded within cell, thus provides better temporal resolution of red fluorescence and promoter activity. Part was assembled using BrickClip assembly (BBF RFC 104) using other biobrick parts as templates. BrickClip assembly is a special case of more general Paperclip assembly method.[1] Reference [1] Trubitsyna, M., Michlewski, G., Cai, Y., Elfick, A. & French, C. E. PaperClip: rapid multi-part DNA assembly from existing libraries. Nucleic Acids Res. 1???8 (2014). doi:10.1093/nar/gku829
mutable notes: no
mutable provenance: Other biobrick parts
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: stop
sequence annotation: start
sequence annotation: stem_loop
sequence annotation: BBa_E1010 w/o stop
sequence annotation: end of LacI coding region (inactive)
sequence annotation: BBa_B0012
sequence annotation: barcode from BBa_E1010
sequence annotation: LacI binding site
sequence annotation: CAP binding site
sequence annotation: polya
sequence annotation: AAV-ssrA tag
sequence annotation: BBa_B0034
sequence annotation: T7 TE
sequence annotation: conserved
sequence annotation: BBa_K1399000
sequence annotation: -10
sequence annotation: -35
sequence annotation: BBa_B0015
sequence annotation: BBa_B0010
sequence annotation: BBa_R0010
sequence: https://synbiohub.org/public/igem/BBa_K1399010_sequence/1

stop
type: SequenceAnnotation
persistent identity: annotation2383945
display id: annotation2383945
version: 1
top level: BBa_K1399010
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1399010/annotation2383945/range2383945/1

https://synbiohub.org/public/igem/BBa_K1399010/annotation2383945/range2383945/1
type: Range
persistent identity: range2383945
display id: range2383945
version: 1
direction: direction/forward
top level: BBa_K1399010
start: 935
end: 937
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2383939
display id: annotation2383939
version: 1
top level: BBa_K1399010
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1399010/annotation2383939/range2383939/1

https://synbiohub.org/public/igem/BBa_K1399010/annotation2383939/range2383939/1
type: Range
persistent identity: range2383939
display id: range2383939
version: 1
direction: direction/forward
top level: BBa_K1399010
start: 173
end: 173
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation2383949
display id: annotation2383949
version: 1
top level: BBa_K1399010
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1399010/annotation2383949/range2383949/1

https://synbiohub.org/public/igem/BBa_K1399010/annotation2383949/range2383949/1
type: Range
persistent identity: range2383949
display id: range2383949
version: 1
direction: direction/forward
top level: BBa_K1399010
start: 985
end: 1028
orientation: inline

BBa_E1010 w/o stop
type: SequenceAnnotation
persistent identity: annotation2383943
display id: annotation2383943
version: 1
top level: BBa_K1399010
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1399010/annotation2383943/range2383943/1

https://synbiohub.org/public/igem/BBa_K1399010/annotation2383943/range2383943/1
type: Range
persistent identity: range2383943
display id: range2383943
version: 1
direction: direction/forward
top level: BBa_K1399010
start: 230
end: 901
orientation: inline

end of LacI coding region (inactive)
type: SequenceAnnotation
persistent identity: annotation2383934
display id: annotation2383934
version: 1
top level: BBa_K1399010
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1399010/annotation2383934/range2383934/1

https://synbiohub.org/public/igem/BBa_K1399010/annotation2383934/range2383934/1
type: Range
persistent identity: range2383934
display id: range2383934
version: 1
direction: direction/forward
top level: BBa_K1399010
start: 1
end: 88
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2383950
display id: annotation2383950
version: 1
top level: BBa_K1399010
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1399010/annotation2383950/range2383950/1

https://synbiohub.org/public/igem/BBa_K1399010/annotation2383950/range2383950/1
type: Range
persistent identity: range2383950
display id: range2383950
version: 1
direction: direction/forward
top level: BBa_K1399010
start: 1062
end: 1102
orientation: inline

barcode from BBa_E1010
type: SequenceAnnotation
persistent identity: annotation2386257
display id: annotation2386257
version: 1
top level: BBa_K1399010
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1399010/annotation2386257/range2386257/1

https://synbiohub.org/public/igem/BBa_K1399010/annotation2386257/range2386257/1
type: Range
persistent identity: range2386257
display id: range2386257
version: 1
direction: direction/forward
top level: BBa_K1399010
start: 941
end: 965
orientation: inline

LacI binding site
type: SequenceAnnotation
persistent identity: annotation2383938
display id: annotation2383938
version: 1
top level: BBa_K1399010
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1399010/annotation2383938/range2383938/1

https://synbiohub.org/public/igem/BBa_K1399010/annotation2383938/range2383938/1
type: Range
persistent identity: range2383938
display id: range2383938
version: 1
direction: direction/forward
top level: BBa_K1399010
start: 166
end: 200
orientation: inline

CAP binding site
type: SequenceAnnotation
persistent identity: annotation2383935
display id: annotation2383935
version: 1
top level: BBa_K1399010
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1399010/annotation2383935/range2383935/1

https://synbiohub.org/public/igem/BBa_K1399010/annotation2383935/range2383935/1
type: Range
persistent identity: range2383935
display id: range2383935
version: 1
direction: direction/forward
top level: BBa_K1399010
start: 89
end: 126
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2383942
display id: annotation2383942
version: 1
top level: BBa_K1399010
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1399010/annotation2383942/range2383942/1

https://synbiohub.org/public/igem/BBa_K1399010/annotation2383942/range2383942/1
type: Range
persistent identity: range2383942
display id: range2383942
version: 1
direction: direction/forward
top level: BBa_K1399010
start: 227
end: 229
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation2383952
display id: annotation2383952
version: 1
top level: BBa_K1399010
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_K1399010/annotation2383952/range2383952/1

https://synbiohub.org/public/igem/BBa_K1399010/annotation2383952/range2383952/1
type: Range
persistent identity: range2383952
display id: range2383952
version: 1
direction: direction/forward
top level: BBa_K1399010
start: 1089
end: 1102
orientation: inline

AAV-ssrA tag
type: SequenceAnnotation
persistent identity: annotation2383944
display id: annotation2383944
version: 1
top level: BBa_K1399010
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1399010/annotation2383944/range2383944/1

https://synbiohub.org/public/igem/BBa_K1399010/annotation2383944/range2383944/1
type: Range
persistent identity: range2383944
display id: range2383944
version: 1
direction: direction/forward
top level: BBa_K1399010
start: 902
end: 934
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2383941
display id: annotation2383941
version: 1
top level: BBa_K1399010
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1399010/annotation2383941/range2383941/1

https://synbiohub.org/public/igem/BBa_K1399010/annotation2383941/range2383941/1
type: Range
persistent identity: range2383941
display id: range2383941
version: 1
direction: direction/reverse
top level: BBa_K1399010
start: 209
end: 220
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation2383951
display id: annotation2383951
version: 1
top level: BBa_K1399010
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1399010/annotation2383951/range2383951/1

https://synbiohub.org/public/igem/BBa_K1399010/annotation2383951/range2383951/1
type: Range
persistent identity: range2383951
display id: range2383951
version: 1
direction: direction/forward
top level: BBa_K1399010
start: 1069
end: 1088
orientation: inline

conserved
type: SequenceAnnotation
persistent identity: annotation2383940
display id: annotation2383940
version: 1
top level: BBa_K1399010
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K1399010/annotation2383940/range2383940/1

https://synbiohub.org/public/igem/BBa_K1399010/annotation2383940/range2383940/1
type: Range
persistent identity: range2383940
display id: range2383940
version: 1
direction: direction/forward
top level: BBa_K1399010
start: 213
end: 216
orientation: inline

BBa_K1399000
type: SequenceAnnotation
persistent identity: annotation2383947
display id: annotation2383947
version: 1
top level: BBa_K1399010
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1399010/annotation2383947/range2383947/1

https://synbiohub.org/public/igem/BBa_K1399010/annotation2383947/range2383947/1
type: Range
persistent identity: range2383947
display id: range2383947
version: 1
direction: direction/reverse
top level: BBa_K1399010
start: 227
end: 940
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2383946
display id: annotation2383946
version: 1
top level: BBa_K1399010
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1399010/annotation2383946/range2383946/1

https://synbiohub.org/public/igem/BBa_K1399010/annotation2383946/range2383946/1
type: Range
persistent identity: range2383946
display id: range2383946
version: 1
direction: direction/forward
top level: BBa_K1399010
start: 938
end: 940
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2383937
display id: annotation2383937
version: 1
top level: BBa_K1399010
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1399010/annotation2383937/range2383937/1

https://synbiohub.org/public/igem/BBa_K1399010/annotation2383937/range2383937/1
type: Range
persistent identity: range2383937
display id: range2383937
version: 1
direction: direction/forward
top level: BBa_K1399010
start: 161
end: 166
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2383953
display id: annotation2383953
version: 1
top level: BBa_K1399010
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1399010/annotation2383953/range2383953/1

https://synbiohub.org/public/igem/BBa_K1399010/annotation2383953/range2383953/1
type: Range
persistent identity: range2383953
display id: range2383953
version: 1
direction: direction/forward
top level: BBa_K1399010
start: 1095
end: 1095
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2383936
display id: annotation2383936
version: 1
top level: BBa_K1399010
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1399010/annotation2383936/range2383936/1

https://synbiohub.org/public/igem/BBa_K1399010/annotation2383936/range2383936/1
type: Range
persistent identity: range2383936
display id: range2383936
version: 1
direction: direction/forward
top level: BBa_K1399010
start: 137
end: 142
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2383954
display id: annotation2383954
version: 1
top level: BBa_K1399010
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1399010/annotation2383954/range2383954/1

https://synbiohub.org/public/igem/BBa_K1399010/annotation2383954/range2383954/1
type: Range
persistent identity: range2383954
display id: range2383954
version: 1
direction: direction/reverse
top level: BBa_K1399010
start: 974
end: 1102
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2383948
display id: annotation2383948
version: 1
top level: BBa_K1399010
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1399010/annotation2383948/range2383948/1

https://synbiohub.org/public/igem/BBa_K1399010/annotation2383948/range2383948/1
type: Range
persistent identity: range2383948
display id: range2383948
version: 1
direction: direction/forward
top level: BBa_K1399010
start: 974
end: 1053
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation2383933
display id: annotation2383933
version: 1
top level: BBa_K1399010
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1399010/annotation2383933/range2383933/1

https://synbiohub.org/public/igem/BBa_K1399010/annotation2383933/range2383933/1
type: Range
persistent identity: range2383933
display id: range2383933
version: 1
direction: direction/forward
top level: BBa_K1399010
start: 1
end: 200
orientation: inline

BBa_J07034
type: ComponentDefinition
persistent identity: BBa J07034
display id: BBa_J07034
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: FecA' :: GFP reporter
creator: jxyu
created: 2005-07-19T11:00:00Z
modified: 2015-08-31T04:08:19Z
discontinued: false
dominant: false
group u list: _21_
m user id: 0
owner id: 384
owning group id: 21
sample status: Not in stock
status: status/Unavailable
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_J07018
component: BBa_B0012
component: BBa_E0040
component: BBa_B0030
component: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_B0010
sequence annotation: BBa_J07018
sequence annotation: BBa_B0030
sequence annotation: BBa_E0040
sequence: https://synbiohub.org/public/igem/BBa_J07034_sequence/1

BBa_J07018
type: Component
persistent identity: component1589696
display id: component1589696
version: 1
top level: BBa_J07034
access: public
definition: https://synbiohub.org/public/igem/BBa_J07018/1

BBa_B0012
type: Component
persistent identity: component1589722
display id: component1589722
version: 1
top level: BBa_J07034
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_E0040
type: Component
persistent identity: component1589707
display id: component1589707
version: 1
top level: BBa_J07034
access: public
definition: https://synbiohub.org/public/igem/BBa_E0040/1

BBa_B0030
type: Component
persistent identity: component1589704
display id: component1589704
version: 1
top level: BBa_J07034
access: public
definition: https://synbiohub.org/public/igem/BBa_B0030/1

BBa_B0010
type: Component
persistent identity: component1589712
display id: component1589712
version: 1
top level: BBa_J07034
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1589722
display id: annotation1589722
version: 1
top level: BBa_J07034
location: https://synbiohub.org/public/igem/BBa_J07034/annotation1589722/range1589722/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_J07034/annotation1589722/range1589722/1
type: Range
persistent identity: range1589722
display id: range1589722
version: 1
direction: direction/forward
top level: BBa_J07034
start: 3171
end: 3211
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1589712
display id: annotation1589712
version: 1
top level: BBa_J07034
location: https://synbiohub.org/public/igem/BBa_J07034/annotation1589712/range1589712/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_J07034/annotation1589712/range1589712/1
type: Range
persistent identity: range1589712
display id: range1589712
version: 1
direction: direction/forward
top level: BBa_J07034
start: 3083
end: 3162
orientation: inline

BBa_J07018
type: SequenceAnnotation
persistent identity: annotation1589696
display id: annotation1589696
version: 1
top level: BBa_J07034
location: https://synbiohub.org/public/igem/BBa_J07034/annotation1589696/range1589696/1
component: BBa_J07018

https://synbiohub.org/public/igem/BBa_J07034/annotation1589696/range1589696/1
type: Range
persistent identity: range1589696
display id: range1589696
version: 1
direction: direction/forward
top level: BBa_J07034
start: 1
end: 2325
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation1589704
display id: annotation1589704
version: 1
top level: BBa_J07034
location: https://synbiohub.org/public/igem/BBa_J07034/annotation1589704/range1589704/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_J07034/annotation1589704/range1589704/1
type: Range
persistent identity: range1589704
display id: range1589704
version: 1
direction: direction/forward
top level: BBa_J07034
start: 2334
end: 2348
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation1589707
display id: annotation1589707
version: 1
top level: BBa_J07034
location: https://synbiohub.org/public/igem/BBa_J07034/annotation1589707/range1589707/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_J07034/annotation1589707/range1589707/1
type: Range
persistent identity: range1589707
display id: range1589707
version: 1
direction: direction/forward
top level: BBa_J07034
start: 2355
end: 3074
orientation: inline

BBa_J09264
type: ComponentDefinition
persistent identity: BBa J09264
display id: BBa_J09264
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pLacI + GFP device
creator: Jon Badalamenti
created: 2006-10-26T11:00:00Z
modified: 2015-08-31T04:08:21Z
discontinued: false
dominant: true
group u list: _72_40_
m user id: 0
owner id: 475
owning group id: 72
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: R0010.E0240
mutable notes: none.
mutable provenance: This part was constructed from parts available through the registry.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_B0010
component: BBa_E0040
component: BBa_R0010
component: BBa_B0032
component: BBa_B0012
sequence annotation: BBa_B0032
sequence annotation: BBa_B0012
sequence annotation: BBa_R0010
sequence annotation: BBa_B0010
sequence annotation: BBa_E0040
sequence: https://synbiohub.org/public/igem/BBa_J09264_sequence/1

BBa_B0010
type: Component
persistent identity: component1907371
display id: component1907371
version: 1
top level: BBa_J09264
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_E0040
type: Component
persistent identity: component1907370
display id: component1907370
version: 1
top level: BBa_J09264
access: public
definition: https://synbiohub.org/public/igem/BBa_E0040/1

BBa_R0010
type: Component
persistent identity: component1907360
display id: component1907360
version: 1
top level: BBa_J09264
access: public
definition: https://synbiohub.org/public/igem/BBa_R0010/1

BBa_B0032
type: Component
persistent identity: component1907368
display id: component1907368
version: 1
top level: BBa_J09264
access: public
definition: https://synbiohub.org/public/igem/BBa_B0032/1

BBa_B0012
type: Component
persistent identity: component1907373
display id: component1907373
version: 1
top level: BBa_J09264
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation1907368
display id: annotation1907368
version: 1
top level: BBa_J09264
location: https://synbiohub.org/public/igem/BBa_J09264/annotation1907368/range1907368/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_J09264/annotation1907368/range1907368/1
type: Range
persistent identity: range1907368
display id: range1907368
version: 1
direction: direction/forward
top level: BBa_J09264
start: 209
end: 221
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1907373
display id: annotation1907373
version: 1
top level: BBa_J09264
location: https://synbiohub.org/public/igem/BBa_J09264/annotation1907373/range1907373/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_J09264/annotation1907373/range1907373/1
type: Range
persistent identity: range1907373
display id: range1907373
version: 1
direction: direction/forward
top level: BBa_J09264
start: 1044
end: 1084
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation1907360
display id: annotation1907360
version: 1
top level: BBa_J09264
location: https://synbiohub.org/public/igem/BBa_J09264/annotation1907360/range1907360/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_J09264/annotation1907360/range1907360/1
type: Range
persistent identity: range1907360
display id: range1907360
version: 1
direction: direction/forward
top level: BBa_J09264
start: 1
end: 200
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1907371
display id: annotation1907371
version: 1
top level: BBa_J09264
location: https://synbiohub.org/public/igem/BBa_J09264/annotation1907371/range1907371/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_J09264/annotation1907371/range1907371/1
type: Range
persistent identity: range1907371
display id: range1907371
version: 1
direction: direction/forward
top level: BBa_J09264
start: 956
end: 1035
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation1907370
display id: annotation1907370
version: 1
top level: BBa_J09264
location: https://synbiohub.org/public/igem/BBa_J09264/annotation1907370/range1907370/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_J09264/annotation1907370/range1907370/1
type: Range
persistent identity: range1907370
display id: range1907370
version: 1
direction: direction/reverse
top level: BBa_J09264
start: 228
end: 947
orientation: inline

BBa_I741103
type: ComponentDefinition
persistent identity: BBa I741103
display id: BBa_I741103
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter (I741019) and GFP (E0240)
creator: Lucien Weiss
created: 2007-06-21T11:00:00Z
modified: 2015-08-31T04:08:02Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _143_
m user id: 0
owner id: 1763
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_E0040
component: BBa_B0030
component: BBa_I741019
component: BBa_B0010
component: BBa_B0012
sequence annotation: BBa_E0040
sequence annotation: BBa_B0012
sequence annotation: BBa_I741019
sequence annotation: BBa_B0010
sequence annotation: BBa_B0030
sequence: https://synbiohub.org/public/igem/BBa_I741103_sequence/1

BBa_E0040
type: Component
persistent identity: component1935276
display id: component1935276
version: 1
top level: BBa_I741103
access: public
definition: https://synbiohub.org/public/igem/BBa_E0040/1

BBa_B0030
type: Component
persistent identity: component1935273
display id: component1935273
version: 1
top level: BBa_I741103
access: public
definition: https://synbiohub.org/public/igem/BBa_B0030/1

BBa_I741019
type: Component
persistent identity: component1935271
display id: component1935271
version: 1
top level: BBa_I741103
access: public
definition: https://synbiohub.org/public/igem/BBa_I741019/1

BBa_B0010
type: Component
persistent identity: component1935277
display id: component1935277
version: 1
top level: BBa_I741103
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
type: Component
persistent identity: component1935279
display id: component1935279
version: 1
top level: BBa_I741103
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation1935276
display id: annotation1935276
version: 1
top level: BBa_I741103
location: https://synbiohub.org/public/igem/BBa_I741103/annotation1935276/range1935276/1
component: BBa_E0040

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type: Range
persistent identity: range1935276
display id: range1935276
version: 1
direction: direction/reverse
top level: BBa_I741103
start: 161
end: 880
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1935279
display id: annotation1935279
version: 1
top level: BBa_I741103
location: https://synbiohub.org/public/igem/BBa_I741103/annotation1935279/range1935279/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I741103/annotation1935279/range1935279/1
type: Range
persistent identity: range1935279
display id: range1935279
version: 1
direction: direction/forward
top level: BBa_I741103
start: 977
end: 1017
orientation: inline

BBa_I741019
type: SequenceAnnotation
persistent identity: annotation1935271
display id: annotation1935271
version: 1
top level: BBa_I741103
location: https://synbiohub.org/public/igem/BBa_I741103/annotation1935271/range1935271/1
component: BBa_I741019

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type: Range
persistent identity: range1935271
display id: range1935271
version: 1
direction: direction/reverse
top level: BBa_I741103
start: 1
end: 131
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1935277
display id: annotation1935277
version: 1
top level: BBa_I741103
location: https://synbiohub.org/public/igem/BBa_I741103/annotation1935277/range1935277/1
component: BBa_B0010

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type: Range
persistent identity: range1935277
display id: range1935277
version: 1
direction: direction/forward
top level: BBa_I741103
start: 889
end: 968
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation1935273
display id: annotation1935273
version: 1
top level: BBa_I741103
location: https://synbiohub.org/public/igem/BBa_I741103/annotation1935273/range1935273/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_I741103/annotation1935273/range1935273/1
type: Range
persistent identity: range1935273
display id: range1935273
version: 1
direction: direction/forward
top level: BBa_I741103
start: 140
end: 154
orientation: inline

Prm+GFP
type: ComponentDefinition
persistent identity: BBa S03336
display id: BBa_S03336
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Prm+ with GFP as a reporter
creator: ETH Zurich iGEM Team 2005
created: 2005-09-20T11:00:00Z
modified: 2015-05-08T01:14:21Z
discontinued: false
dominant: true
experience: experience/None
group u list: _34_
m user id: 0
owner id: 398
owning group id: 34
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Prm+ promoter of the lambda-system (with OR3 region deactivated over mutation) with a GFP reporter. The purpose is to roughly quantify its relative basal activity compared with the constitutive activity of the Pr promoter.
mutable notes: For more details see Sensing Device and Event Processing Device documentation.
mutable provenance: Registry 7.05
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_E0040
component: BBa_B0034
component: BBa_I12007
component: BBa_B0010
component: BBa_B0012
sequence annotation: BBa_B0034
sequence annotation: BBa_B0012
sequence annotation: BBa_B0010
sequence annotation: BBa_I12007
sequence annotation: BBa_E0040
sequence: https://synbiohub.org/public/igem/BBa_S03336_sequence/1

BBa_E0040
type: Component
persistent identity: component1700299
display id: component1700299
version: 1
top level: Prm+GFP
access: public
definition: https://synbiohub.org/public/igem/BBa_E0040/1

BBa_B0034
type: Component
persistent identity: component1700297
display id: component1700297
version: 1
top level: Prm+GFP
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_I12007
type: Component
persistent identity: component1700292
display id: component1700292
version: 1
top level: Prm+GFP
access: public
definition: https://synbiohub.org/public/igem/BBa_I12007/1

BBa_B0010
type: Component
persistent identity: component1700304
display id: component1700304
version: 1
top level: Prm+GFP
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
type: Component
persistent identity: component1700314
display id: component1700314
version: 1
top level: Prm+GFP
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1700297
display id: annotation1700297
version: 1
top level: Prm+GFP
location: https://synbiohub.org/public/igem/BBa_S03336/annotation1700297/range1700297/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_S03336/annotation1700297/range1700297/1
type: Range
persistent identity: range1700297
display id: range1700297
version: 1
direction: direction/forward
top level: Prm+GFP
start: 91
end: 102
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1700314
display id: annotation1700314
version: 1
top level: Prm+GFP
location: https://synbiohub.org/public/igem/BBa_S03336/annotation1700314/range1700314/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_S03336/annotation1700314/range1700314/1
type: Range
persistent identity: range1700314
display id: range1700314
version: 1
direction: direction/forward
top level: Prm+GFP
start: 925
end: 965
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1700304
display id: annotation1700304
version: 1
top level: Prm+GFP
location: https://synbiohub.org/public/igem/BBa_S03336/annotation1700304/range1700304/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_S03336/annotation1700304/range1700304/1
type: Range
persistent identity: range1700304
display id: range1700304
version: 1
direction: direction/forward
top level: Prm+GFP
start: 837
end: 916
orientation: inline

BBa_I12007
type: SequenceAnnotation
persistent identity: annotation1700292
display id: annotation1700292
version: 1
top level: Prm+GFP
location: https://synbiohub.org/public/igem/BBa_S03336/annotation1700292/range1700292/1
component: BBa_I12007

https://synbiohub.org/public/igem/BBa_S03336/annotation1700292/range1700292/1
type: Range
persistent identity: range1700292
display id: range1700292
version: 1
direction: direction/forward
top level: Prm+GFP
start: 1
end: 82
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation1700299
display id: annotation1700299
version: 1
top level: Prm+GFP
location: https://synbiohub.org/public/igem/BBa_S03336/annotation1700299/range1700299/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_S03336/annotation1700299/range1700299/1
type: Range
persistent identity: range1700299
display id: range1700299
version: 1
direction: direction/forward
top level: Prm+GFP
start: 109
end: 828
orientation: inline

BBa_J14430
type: ComponentDefinition
persistent identity: BBa J14430
display id: BBa_J14430
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: GFP coding device
creator: Meagan Lizarazo
created: 2007-01-31T12:00:00Z
modified: 2015-08-31T04:08:31Z
discontinued: false
dominant: false
group u list: _42_1_
m user id: 0
owner id: 457
owning group id: 67
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This is a twin of BBa_J04430. The Registry is using the assembly of this part as a control in robotic assembly. Since it is a GFP reporter we can assemble it and use the results as an indication of whether or not our assembly was successful.
mutable notes: N/A
mutable provenance: Robotic assembly
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_E0040
component: BBa_R0010
component: BBa_B0034
component: BBa_B0010
component: BBa_B0012
sequence annotation: BBa_R0010
sequence annotation: BBa_E0040
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_J14430_sequence/1

BBa_E0040
type: Component
persistent identity: component1911686
display id: component1911686
version: 1
top level: BBa_J14430
access: public
definition: https://synbiohub.org/public/igem/BBa_E0040/1

BBa_R0010
type: Component
persistent identity: component1911677
display id: component1911677
version: 1
top level: BBa_J14430
access: public
definition: https://synbiohub.org/public/igem/BBa_R0010/1

BBa_B0034
type: Component
persistent identity: component1911685
display id: component1911685
version: 1
top level: BBa_J14430
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0010
type: Component
persistent identity: component1911687
display id: component1911687
version: 1
top level: BBa_J14430
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
type: Component
persistent identity: component1911689
display id: component1911689
version: 1
top level: BBa_J14430
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation1911677
display id: annotation1911677
version: 1
top level: BBa_J14430
location: https://synbiohub.org/public/igem/BBa_J14430/annotation1911677/range1911677/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_J14430/annotation1911677/range1911677/1
type: Range
persistent identity: range1911677
display id: range1911677
version: 1
direction: direction/forward
top level: BBa_J14430
start: 1
end: 200
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation1911686
display id: annotation1911686
version: 1
top level: BBa_J14430
location: https://synbiohub.org/public/igem/BBa_J14430/annotation1911686/range1911686/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_J14430/annotation1911686/range1911686/1
type: Range
persistent identity: range1911686
display id: range1911686
version: 1
direction: direction/reverse
top level: BBa_J14430
start: 227
end: 946
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1911687
display id: annotation1911687
version: 1
top level: BBa_J14430
location: https://synbiohub.org/public/igem/BBa_J14430/annotation1911687/range1911687/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_J14430/annotation1911687/range1911687/1
type: Range
persistent identity: range1911687
display id: range1911687
version: 1
direction: direction/forward
top level: BBa_J14430
start: 955
end: 1034
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1911689
display id: annotation1911689
version: 1
top level: BBa_J14430
location: https://synbiohub.org/public/igem/BBa_J14430/annotation1911689/range1911689/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_J14430/annotation1911689/range1911689/1
type: Range
persistent identity: range1911689
display id: range1911689
version: 1
direction: direction/forward
top level: BBa_J14430
start: 1043
end: 1083
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1911685
display id: annotation1911685
version: 1
top level: BBa_J14430
location: https://synbiohub.org/public/igem/BBa_J14430/annotation1911685/range1911685/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_J14430/annotation1911685/range1911685/1
type: Range
persistent identity: range1911685
display id: range1911685
version: 1
direction: direction/reverse
top level: BBa_J14430
start: 209
end: 220
orientation: inline

BBa_K1399023
type: ComponentDefinition
persistent identity: BBa K1399023
display id: BBa_K1399023
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ptet-GFP(DAS)
creator: Anna Stikane
created: 2014-09-18T11:00:00Z
modified: 2015-05-08T01:10:16Z
discontinued: false
dominant: false
group u list: _1777_
m user id: 0
owner id: 22477
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: Tetracycline/aTc inducible promoter with GFP reporter tagged with DAS-ssrA degradation tag followed by terminator. The tagged GFP is actively degraded within cell, thus provides better temporal resolution of green fluorescence and promoter activity.Presence of SspB is crucial for effective protein degradation.
mutable notes: Part was assembled using BrickClip assembly (BBF RFC104) using other biobrick parts as templates. BrickClip assembly is a special case of more general Paperclip assembly method.[1]
mutable provenance: other biobricks
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_B0034
component: BBa_B0015
component: BBa_K1399008
component: BBa_R0040
sequence annotation: BBa_K1399008
sequence annotation: BBa_R0040
sequence annotation: BBa_B0034
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_K1399023_sequence/1

BBa_B0034
type: Component
persistent identity: component2384305
display id: component2384305
version: 1
top level: BBa_K1399023
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0015
type: Component
persistent identity: component2384318
display id: component2384318
version: 1
top level: BBa_K1399023
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_K1399008
type: Component
persistent identity: component2384311
display id: component2384311
version: 1
top level: BBa_K1399023
access: public
definition: https://synbiohub.org/public/igem/BBa_K1399008/1

BBa_R0040
type: Component
persistent identity: component2384299
display id: component2384299
version: 1
top level: BBa_K1399023
access: public
definition: https://synbiohub.org/public/igem/BBa_R0040/1

BBa_K1399008
type: SequenceAnnotation
persistent identity: annotation2384311
display id: annotation2384311
version: 1
top level: BBa_K1399023
location: https://synbiohub.org/public/igem/BBa_K1399023/annotation2384311/range2384311/1
component: BBa_K1399008

https://synbiohub.org/public/igem/BBa_K1399023/annotation2384311/range2384311/1
type: Range
persistent identity: range2384311
display id: range2384311
version: 1
direction: direction/reverse
top level: BBa_K1399023
start: 81
end: 833
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2384299
display id: annotation2384299
version: 1
top level: BBa_K1399023
location: https://synbiohub.org/public/igem/BBa_K1399023/annotation2384299/range2384299/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_K1399023/annotation2384299/range2384299/1
type: Range
persistent identity: range2384299
display id: range2384299
version: 1
direction: direction/forward
top level: BBa_K1399023
start: 1
end: 54
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2384305
display id: annotation2384305
version: 1
top level: BBa_K1399023
location: https://synbiohub.org/public/igem/BBa_K1399023/annotation2384305/range2384305/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1399023/annotation2384305/range2384305/1
type: Range
persistent identity: range2384305
display id: range2384305
version: 1
direction: direction/reverse
top level: BBa_K1399023
start: 63
end: 74
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2384318
display id: annotation2384318
version: 1
top level: BBa_K1399023
location: https://synbiohub.org/public/igem/BBa_K1399023/annotation2384318/range2384318/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K1399023/annotation2384318/range2384318/1
type: Range
persistent identity: range2384318
display id: range2384318
version: 1
direction: direction/reverse
top level: BBa_K1399023
start: 842
end: 970
orientation: inline

BBa_J04450
type: ComponentDefinition
persistent identity: BBa J04450
display id: BBa_J04450
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RFP Coding Device
creator: Tamar Odle
created: 2005-06-08T11:00:00Z
modified: 2015-08-31T04:08:14Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _16_
m user id: 0
owner id: 328
owning group id: 16
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Contains an IPTG inducible promoter an RBS, RFP, and terminator.
mutable provenance: Davidson Synth-Aces
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_B0034
component: BBa_R0010
component: BBa_B0012
component: BBa_B0010
component: BBa_E1010
sequence annotation: BBa_E1010
sequence annotation: BBa_R0010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0034
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_J04450_sequence/1

BBa_B0034
type: Component
persistent identity: component1509404
display id: component1509404
version: 1
top level: BBa_J04450
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_R0010
type: Component
persistent identity: component1509394
display id: component1509394
version: 1
top level: BBa_J04450
access: public
definition: https://synbiohub.org/public/igem/BBa_R0010/1

BBa_B0012
type: Component
persistent identity: component1509427
display id: component1509427
version: 1
top level: BBa_J04450
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0010
type: Component
persistent identity: component1509417
display id: component1509417
version: 1
top level: BBa_J04450
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_E1010
type: Component
persistent identity: component1509411
display id: component1509411
version: 1
top level: BBa_J04450
access: public
definition: https://synbiohub.org/public/igem/BBa_E1010/1

BBa_E1010
type: SequenceAnnotation
persistent identity: annotation1509411
display id: annotation1509411
version: 1
top level: BBa_J04450
location: https://synbiohub.org/public/igem/BBa_J04450/annotation1509411/range1509411/1
component: BBa_E1010

https://synbiohub.org/public/igem/BBa_J04450/annotation1509411/range1509411/1
type: Range
persistent identity: range1509411
display id: range1509411
version: 1
direction: direction/forward
top level: BBa_J04450
start: 227
end: 907
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation1509394
display id: annotation1509394
version: 1
top level: BBa_J04450
location: https://synbiohub.org/public/igem/BBa_J04450/annotation1509394/range1509394/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_J04450/annotation1509394/range1509394/1
type: Range
persistent identity: range1509394
display id: range1509394
version: 1
direction: direction/forward
top level: BBa_J04450
start: 1
end: 200
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1509417
display id: annotation1509417
version: 1
top level: BBa_J04450
location: https://synbiohub.org/public/igem/BBa_J04450/annotation1509417/range1509417/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_J04450/annotation1509417/range1509417/1
type: Range
persistent identity: range1509417
display id: range1509417
version: 1
direction: direction/forward
top level: BBa_J04450
start: 941
end: 1020
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1509404
display id: annotation1509404
version: 1
top level: BBa_J04450
location: https://synbiohub.org/public/igem/BBa_J04450/annotation1509404/range1509404/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_J04450/annotation1509404/range1509404/1
type: Range
persistent identity: range1509404
display id: range1509404
version: 1
direction: direction/forward
top level: BBa_J04450
start: 209
end: 220
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1509427
display id: annotation1509427
version: 1
top level: BBa_J04450
location: https://synbiohub.org/public/igem/BBa_J04450/annotation1509427/range1509427/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_J04450/annotation1509427/range1509427/1
type: Range
persistent identity: range1509427
display id: range1509427
version: 1
direction: direction/forward
top level: BBa_J04450
start: 1029
end: 1069
orientation: inline

BBa_J06103
type: ComponentDefinition
persistent identity: BBa J06103
display id: BBa_J06103
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: tetR repressible ECFP reporter
creator: kxjin
created: 2005-06-20T11:00:00Z
modified: 2015-08-31T04:08:16Z
discontinued: false
dominant: false
experience: experience/None
group u list: _20_
m user id: 0
owner id: 368
owning group id: 20
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: -- No description --
owned by: james
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star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_R0040
component: BBa_B0012
component: BBa_B0010
component: BBa_B0034
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sequence annotation: BBa_B0012
sequence annotation: BBa_E0022
sequence annotation: BBa_B0010
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BBa_R0040
type: Component
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definition: https://synbiohub.org/public/igem/BBa_R0040/1

BBa_B0012
type: Component
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version: 1
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access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0010
type: Component
persistent identity: component1528515
display id: component1528515
version: 1
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access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0034
type: Component
persistent identity: component1528495
display id: component1528495
version: 1
top level: BBa_J06103
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_E0022
type: Component
persistent identity: component1528507
display id: component1528507
version: 1
top level: BBa_J06103
access: public
definition: https://synbiohub.org/public/igem/BBa_E0022/1

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1528487
display id: annotation1528487
version: 1
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component: BBa_R0040

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type: Range
persistent identity: range1528487
display id: range1528487
version: 1
direction: direction/forward
top level: BBa_J06103
start: 1
end: 54
orientation: inline

BBa_B0012
type: SequenceAnnotation
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display id: annotation1528525
version: 1
top level: BBa_J06103
location: https://synbiohub.org/public/igem/BBa_J06103/annotation1528525/range1528525/1
component: BBa_B0012

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type: Range
persistent identity: range1528525
display id: range1528525
version: 1
direction: direction/forward
top level: BBa_J06103
start: 939
end: 979
orientation: inline

BBa_E0022
type: SequenceAnnotation
persistent identity: annotation1528507
display id: annotation1528507
version: 1
top level: BBa_J06103
location: https://synbiohub.org/public/igem/BBa_J06103/annotation1528507/range1528507/1
component: BBa_E0022

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type: Range
persistent identity: range1528507
display id: range1528507
version: 1
direction: direction/forward
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start: 81
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orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1528515
display id: annotation1528515
version: 1
top level: BBa_J06103
location: https://synbiohub.org/public/igem/BBa_J06103/annotation1528515/range1528515/1
component: BBa_B0010

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type: Range
persistent identity: range1528515
display id: range1528515
version: 1
direction: direction/forward
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start: 851
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BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1528495
display id: annotation1528495
version: 1
top level: BBa_J06103
location: https://synbiohub.org/public/igem/BBa_J06103/annotation1528495/range1528495/1
component: BBa_B0034

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type: Range
persistent identity: range1528495
display id: range1528495
version: 1
direction: direction/forward
top level: BBa_J06103
start: 63
end: 74
orientation: inline

BBa_I13521
type: ComponentDefinition
persistent identity: BBa I13521
display id: BBa_I13521
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ptet mRFP
creator: jkm
created: 2005-06-21T11:00:00Z
modified: 2015-08-31T04:07:34Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _11_
m user id: 0
owner id: 253
owning group id: 6
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Constitutive on mRFP (positive control)
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_B0012
component: BBa_E1010
component: BBa_B0010
component: BBa_B0034
component: BBa_R0040
sequence annotation: BBa_B0034
sequence annotation: BBa_R0040
sequence annotation: BBa_B0010
sequence annotation: BBa_E1010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_I13521_sequence/1

BBa_B0012
type: Component
persistent identity: component1532589
display id: component1532589
version: 1
top level: BBa_I13521
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_E1010
type: Component
persistent identity: component1532573
display id: component1532573
version: 1
top level: BBa_I13521
access: public
definition: https://synbiohub.org/public/igem/BBa_E1010/1

BBa_B0010
type: Component
persistent identity: component1532579
display id: component1532579
version: 1
top level: BBa_I13521
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0034
type: Component
persistent identity: component1532566
display id: component1532566
version: 1
top level: BBa_I13521
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_R0040
type: Component
persistent identity: component1532558
display id: component1532558
version: 1
top level: BBa_I13521
access: public
definition: https://synbiohub.org/public/igem/BBa_R0040/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1532566
display id: annotation1532566
version: 1
top level: BBa_I13521
location: https://synbiohub.org/public/igem/BBa_I13521/annotation1532566/range1532566/1
component: BBa_B0034

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type: Range
persistent identity: range1532566
display id: range1532566
version: 1
direction: direction/forward
top level: BBa_I13521
start: 63
end: 74
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1532558
display id: annotation1532558
version: 1
top level: BBa_I13521
location: https://synbiohub.org/public/igem/BBa_I13521/annotation1532558/range1532558/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_I13521/annotation1532558/range1532558/1
type: Range
persistent identity: range1532558
display id: range1532558
version: 1
direction: direction/forward
top level: BBa_I13521
start: 1
end: 54
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1532579
display id: annotation1532579
version: 1
top level: BBa_I13521
location: https://synbiohub.org/public/igem/BBa_I13521/annotation1532579/range1532579/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I13521/annotation1532579/range1532579/1
type: Range
persistent identity: range1532579
display id: range1532579
version: 1
direction: direction/forward
top level: BBa_I13521
start: 795
end: 874
orientation: inline

BBa_E1010
type: SequenceAnnotation
persistent identity: annotation1532573
display id: annotation1532573
version: 1
top level: BBa_I13521
location: https://synbiohub.org/public/igem/BBa_I13521/annotation1532573/range1532573/1
component: BBa_E1010

https://synbiohub.org/public/igem/BBa_I13521/annotation1532573/range1532573/1
type: Range
persistent identity: range1532573
display id: range1532573
version: 1
direction: direction/forward
top level: BBa_I13521
start: 81
end: 761
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1532589
display id: annotation1532589
version: 1
top level: BBa_I13521
location: https://synbiohub.org/public/igem/BBa_I13521/annotation1532589/range1532589/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I13521/annotation1532589/range1532589/1
type: Range
persistent identity: range1532589
display id: range1532589
version: 1
direction: direction/forward
top level: BBa_I13521
start: 883
end: 923
orientation: inline

BBa_J47861
type: ComponentDefinition
persistent identity: BBa J47861
display id: BBa_J47861
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pLacI + ECFP (LVA+)
creator: Jon Badalamenti
created: 2006-10-26T11:00:00Z
modified: 2015-08-31T04:08:51Z
discontinued: false
dominant: false
group u list: _72_40_
m user id: 0
owner id: 475
owning group id: 72
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: R0010.B0030.E0022.B0015
mutable notes: none.
mutable provenance: This part was constructed from parts available through the registry.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_B0010
component: BBa_R0010
component: BBa_E0022
component: BBa_B0030
component: BBa_B0012
sequence annotation: BBa_B0030
sequence annotation: BBa_R0010
sequence annotation: BBa_B0012
sequence annotation: BBa_E0022
sequence annotation: BBa_B0010
sequence: https://synbiohub.org/public/igem/BBa_J47861_sequence/1

BBa_B0010
type: Component
persistent identity: component1907701
display id: component1907701
version: 1
top level: BBa_J47861
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_R0010
type: Component
persistent identity: component1907686
display id: component1907686
version: 1
top level: BBa_J47861
access: public
definition: https://synbiohub.org/public/igem/BBa_R0010/1

BBa_E0022
type: Component
persistent identity: component1907697
display id: component1907697
version: 1
top level: BBa_J47861
access: public
definition: https://synbiohub.org/public/igem/BBa_E0022/1

BBa_B0030
type: Component
persistent identity: component1907694
display id: component1907694
version: 1
top level: BBa_J47861
access: public
definition: https://synbiohub.org/public/igem/BBa_B0030/1

BBa_B0012
type: Component
persistent identity: component1907703
display id: component1907703
version: 1
top level: BBa_J47861
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation1907694
display id: annotation1907694
version: 1
top level: BBa_J47861
location: https://synbiohub.org/public/igem/BBa_J47861/annotation1907694/range1907694/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_J47861/annotation1907694/range1907694/1
type: Range
persistent identity: range1907694
display id: range1907694
version: 1
direction: direction/forward
top level: BBa_J47861
start: 209
end: 223
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation1907686
display id: annotation1907686
version: 1
top level: BBa_J47861
location: https://synbiohub.org/public/igem/BBa_J47861/annotation1907686/range1907686/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_J47861/annotation1907686/range1907686/1
type: Range
persistent identity: range1907686
display id: range1907686
version: 1
direction: direction/forward
top level: BBa_J47861
start: 1
end: 200
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1907703
display id: annotation1907703
version: 1
top level: BBa_J47861
location: https://synbiohub.org/public/igem/BBa_J47861/annotation1907703/range1907703/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_J47861/annotation1907703/range1907703/1
type: Range
persistent identity: range1907703
display id: range1907703
version: 1
direction: direction/forward
top level: BBa_J47861
start: 1088
end: 1128
orientation: inline

BBa_E0022
type: SequenceAnnotation
persistent identity: annotation1907697
display id: annotation1907697
version: 1
top level: BBa_J47861
location: https://synbiohub.org/public/igem/BBa_J47861/annotation1907697/range1907697/1
component: BBa_E0022

https://synbiohub.org/public/igem/BBa_J47861/annotation1907697/range1907697/1
type: Range
persistent identity: range1907697
display id: range1907697
version: 1
direction: direction/forward
top level: BBa_J47861
start: 230
end: 991
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1907701
display id: annotation1907701
version: 1
top level: BBa_J47861
location: https://synbiohub.org/public/igem/BBa_J47861/annotation1907701/range1907701/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_J47861/annotation1907701/range1907701/1
type: Range
persistent identity: range1907701
display id: range1907701
version: 1
direction: direction/forward
top level: BBa_J47861
start: 1000
end: 1079
orientation: inline

BBa_K1067001
type: ComponentDefinition
persistent identity: BBa K1067001
display id: BBa_K1067001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Twin-arginine translocation reporter
creator: Kristian Davidsen
created: 2013-09-18T11:00:00Z
modified: 2015-05-08T01:09:00Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1376_
m user id: 0
owner id: 16028
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: bbbb
mutable notes: rrrr
mutable provenance: eeee
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: GFP SF
sequence annotation: stop
sequence annotation: start
sequence annotation: rbs
sequence annotation: TAT signal peptide
sequence annotation: Gap
sequence annotation: RFP mCherry
sequence: https://synbiohub.org/public/igem/BBa_K1067001_sequence/1

GFP SF
type: SequenceAnnotation
persistent identity: annotation2359398
display id: annotation2359398
version: 1
top level: BBa_K1067001
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1067001/annotation2359398/range2359398/1

https://synbiohub.org/public/igem/BBa_K1067001/annotation2359398/range2359398/1
type: Range
persistent identity: range2359398
display id: range2359398
version: 1
direction: direction/forward
top level: BBa_K1067001
start: 140
end: 853
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2359406
display id: annotation2359406
version: 1
top level: BBa_K1067001
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1067001/annotation2359406/range2359406/1

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type: Range
persistent identity: range2359406
display id: range2359406
version: 1
direction: direction/forward
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start: 1582
end: 1584
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2359401
display id: annotation2359401
version: 1
top level: BBa_K1067001
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1067001/annotation2359401/range2359401/1

https://synbiohub.org/public/igem/BBa_K1067001/annotation2359401/range2359401/1
type: Range
persistent identity: range2359401
display id: range2359401
version: 1
direction: direction/forward
top level: BBa_K1067001
start: 23
end: 25
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation2359400
display id: annotation2359400
version: 1
top level: BBa_K1067001
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1067001/annotation2359400/range2359400/1

https://synbiohub.org/public/igem/BBa_K1067001/annotation2359400/range2359400/1
type: Range
persistent identity: range2359400
display id: range2359400
version: 1
direction: direction/forward
top level: BBa_K1067001
start: 862
end: 866
orientation: inline

TAT signal peptide
type: SequenceAnnotation
persistent identity: annotation2359397
display id: annotation2359397
version: 1
top level: BBa_K1067001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1067001/annotation2359397/range2359397/1

https://synbiohub.org/public/igem/BBa_K1067001/annotation2359397/range2359397/1
type: Range
persistent identity: range2359397
display id: range2359397
version: 1
direction: direction/forward
top level: BBa_K1067001
start: 23
end: 139
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2359404
display id: annotation2359404
version: 1
top level: BBa_K1067001
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1067001/annotation2359404/range2359404/1

https://synbiohub.org/public/igem/BBa_K1067001/annotation2359404/range2359404/1
type: Range
persistent identity: range2359404
display id: range2359404
version: 1
direction: direction/forward
top level: BBa_K1067001
start: 874
end: 876
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2359402
display id: annotation2359402
version: 1
top level: BBa_K1067001
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1067001/annotation2359402/range2359402/1

https://synbiohub.org/public/igem/BBa_K1067001/annotation2359402/range2359402/1
type: Range
persistent identity: range2359402
display id: range2359402
version: 1
direction: direction/forward
top level: BBa_K1067001
start: 851
end: 853
orientation: inline

Gap
type: SequenceAnnotation
persistent identity: annotation2359399
display id: annotation2359399
version: 1
top level: BBa_K1067001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1067001/annotation2359399/range2359399/1

https://synbiohub.org/public/igem/BBa_K1067001/annotation2359399/range2359399/1
type: Range
persistent identity: range2359399
display id: range2359399
version: 1
direction: direction/forward
top level: BBa_K1067001
start: 854
end: 873
orientation: inline

RFP mCherry
type: SequenceAnnotation
persistent identity: annotation2359407
display id: annotation2359407
version: 1
top level: BBa_K1067001
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1067001/annotation2359407/range2359407/1

https://synbiohub.org/public/igem/BBa_K1067001/annotation2359407/range2359407/1
type: Range
persistent identity: range2359407
display id: range2359407
version: 1
direction: direction/forward
top level: BBa_K1067001
start: 874
end: 1584
orientation: inline

Pulsegen t
type: ComponentDefinition
persistent identity: BBa J03102
display id: BBa_J03102
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: EYFP, cI activated/cI434 repressed promoter
creator: James Godman
created: 2005-10-04T11:00:00Z
modified: 2015-08-31T04:08:13Z
discontinued: false
dominant: false
experience: experience/None
group u list: _15_
m user id: 0
owner id: 405
owning group id: 15
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Designed to test whether our Pulse Generation circuit can give transient gene expression
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_E0030
component: BBa_B0010
component: BBa_B0034
component: BBa_B0012
component: BBa_I12006
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_B0034
sequence annotation: BBa_I12006
sequence annotation: BBa_E0030
sequence: https://synbiohub.org/public/igem/BBa_J03102_sequence/1

BBa_E0030
type: Component
persistent identity: component1718253
display id: component1718253
version: 1
top level: Pulsegen t
access: public
definition: https://synbiohub.org/public/igem/BBa_E0030/1

BBa_B0010
type: Component
persistent identity: component1718258
display id: component1718258
version: 1
top level: Pulsegen t
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0034
type: Component
persistent identity: component1718251
display id: component1718251
version: 1
top level: Pulsegen t
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0012
type: Component
persistent identity: component1718268
display id: component1718268
version: 1
top level: Pulsegen t
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_I12006
type: Component
persistent identity: component1718246
display id: component1718246
version: 1
top level: Pulsegen t
access: public
definition: https://synbiohub.org/public/igem/BBa_I12006/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1718258
display id: annotation1718258
version: 1
top level: Pulsegen t
location: https://synbiohub.org/public/igem/BBa_J03102/annotation1718258/range1718258/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_J03102/annotation1718258/range1718258/1
type: Range
persistent identity: range1718258
display id: range1718258
version: 1
direction: direction/forward
top level: Pulsegen t
start: 840
end: 919
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1718268
display id: annotation1718268
version: 1
top level: Pulsegen t
location: https://synbiohub.org/public/igem/BBa_J03102/annotation1718268/range1718268/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_J03102/annotation1718268/range1718268/1
type: Range
persistent identity: range1718268
display id: range1718268
version: 1
direction: direction/forward
top level: Pulsegen t
start: 928
end: 968
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1718251
display id: annotation1718251
version: 1
top level: Pulsegen t
location: https://synbiohub.org/public/igem/BBa_J03102/annotation1718251/range1718251/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_J03102/annotation1718251/range1718251/1
type: Range
persistent identity: range1718251
display id: range1718251
version: 1
direction: direction/forward
top level: Pulsegen t
start: 91
end: 102
orientation: inline

BBa_I12006
type: SequenceAnnotation
persistent identity: annotation1718246
display id: annotation1718246
version: 1
top level: Pulsegen t
location: https://synbiohub.org/public/igem/BBa_J03102/annotation1718246/range1718246/1
component: BBa_I12006

https://synbiohub.org/public/igem/BBa_J03102/annotation1718246/range1718246/1
type: Range
persistent identity: range1718246
display id: range1718246
version: 1
direction: direction/forward
top level: Pulsegen t
start: 1
end: 82
orientation: inline

BBa_E0030
type: SequenceAnnotation
persistent identity: annotation1718253
display id: annotation1718253
version: 1
top level: Pulsegen t
location: https://synbiohub.org/public/igem/BBa_J03102/annotation1718253/range1718253/1
component: BBa_E0030

https://synbiohub.org/public/igem/BBa_J03102/annotation1718253/range1718253/1
type: Range
persistent identity: range1718253
display id: range1718253
version: 1
direction: direction/forward
top level: Pulsegen t
start: 109
end: 831
orientation: inline

BBa_I13580
type: ComponentDefinition
persistent identity: BBa I13580
display id: BBa_I13580
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: 1/2INS + RFP
creator: Jason Kelly
created: 2007-06-13T11:00:00Z
modified: 2015-08-31T04:07:35Z
discontinued: false
dominant: false
group u list: _11_84_
m user id: 0
owner id: 2
owning group id: 84
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Construct for testing the empty screening plasmid. This is the downstream part of the SP (psb1A0) after a part is inserted.
mutable notes: .
mutable provenance: .
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_I13579
component: BBa_E1010
component: BBa_B0015
component: BBa_B0034
sequence annotation: BBa_I13579
sequence annotation: BBa_B0034
sequence annotation: BBa_E1010
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_I13580_sequence/1

BBa_I13579
type: Component
persistent identity: component2252730
display id: component2252730
version: 1
top level: BBa_I13580
access: public
definition: https://synbiohub.org/public/igem/BBa_I13579/1

BBa_E1010
type: Component
persistent identity: component2252735
display id: component2252735
version: 1
top level: BBa_I13580
access: public
definition: https://synbiohub.org/public/igem/BBa_E1010/1

BBa_B0015
type: Component
persistent identity: component2252742
display id: component2252742
version: 1
top level: BBa_I13580
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_B0034
type: Component
persistent identity: component2252732
display id: component2252732
version: 1
top level: BBa_I13580
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_I13579
type: SequenceAnnotation
persistent identity: annotation2252730
display id: annotation2252730
version: 1
top level: BBa_I13580
location: https://synbiohub.org/public/igem/BBa_I13580/annotation2252730/range2252730/1
component: BBa_I13579

https://synbiohub.org/public/igem/BBa_I13580/annotation2252730/range2252730/1
type: Range
persistent identity: range2252730
display id: range2252730
version: 1
direction: direction/reverse
top level: BBa_I13580
start: 1
end: 72
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2252732
display id: annotation2252732
version: 1
top level: BBa_I13580
location: https://synbiohub.org/public/igem/BBa_I13580/annotation2252732/range2252732/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I13580/annotation2252732/range2252732/1
type: Range
persistent identity: range2252732
display id: range2252732
version: 1
direction: direction/reverse
top level: BBa_I13580
start: 81
end: 92
orientation: inline

BBa_E1010
type: SequenceAnnotation
persistent identity: annotation2252735
display id: annotation2252735
version: 1
top level: BBa_I13580
location: https://synbiohub.org/public/igem/BBa_I13580/annotation2252735/range2252735/1
component: BBa_E1010

https://synbiohub.org/public/igem/BBa_I13580/annotation2252735/range2252735/1
type: Range
persistent identity: range2252735
display id: range2252735
version: 1
direction: direction/reverse
top level: BBa_I13580
start: 99
end: 804
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2252742
display id: annotation2252742
version: 1
top level: BBa_I13580
location: https://synbiohub.org/public/igem/BBa_I13580/annotation2252742/range2252742/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_I13580/annotation2252742/range2252742/1
type: Range
persistent identity: range2252742
display id: range2252742
version: 1
direction: direction/reverse
top level: BBa_I13580
start: 813
end: 941
orientation: inline

BBa_J69502
type: ComponentDefinition
persistent identity: BBa J69502
display id: BBa_J69502
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CI-repressible mCherry Reporter
creator: James Brown
created: 2007-03-28T11:00:00Z
modified: 2015-05-08T01:08:19Z
discontinued: false
dominant: true
group u list: _101_
m user id: 0
owner id: 740
owning group id: 101
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Test construct to validate part J06702 before use in future assemblies
mutable notes: NA
mutable provenance: Existing registry parts
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_R0051
component: BBa_B0034
component: BBa_B0012
component: BBa_J06504
component: BBa_B0010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_B0034
sequence annotation: BBa_R0051
sequence annotation: BBa_J06504
sequence: https://synbiohub.org/public/igem/BBa_J69502_sequence/1

BBa_R0051
type: Component
persistent identity: component1923081
display id: component1923081
version: 1
top level: BBa_J69502
access: public
definition: https://synbiohub.org/public/igem/BBa_R0051/1

BBa_B0034
type: Component
persistent identity: component1923086
display id: component1923086
version: 1
top level: BBa_J69502
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0012
type: Component
persistent identity: component1923092
display id: component1923092
version: 1
top level: BBa_J69502
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_J06504
type: Component
persistent identity: component1923089
display id: component1923089
version: 1
top level: BBa_J69502
access: public
definition: https://synbiohub.org/public/igem/BBa_J06504/1

BBa_B0010
type: Component
persistent identity: component1923090
display id: component1923090
version: 1
top level: BBa_J69502
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1923090
display id: annotation1923090
version: 1
top level: BBa_J69502
location: https://synbiohub.org/public/igem/BBa_J69502/annotation1923090/range1923090/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_J69502/annotation1923090/range1923090/1
type: Range
persistent identity: range1923090
display id: range1923090
version: 1
direction: direction/forward
top level: BBa_J69502
start: 798
end: 877
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1923092
display id: annotation1923092
version: 1
top level: BBa_J69502
location: https://synbiohub.org/public/igem/BBa_J69502/annotation1923092/range1923092/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_J69502/annotation1923092/range1923092/1
type: Range
persistent identity: range1923092
display id: range1923092
version: 1
direction: direction/forward
top level: BBa_J69502
start: 886
end: 926
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1923086
display id: annotation1923086
version: 1
top level: BBa_J69502
location: https://synbiohub.org/public/igem/BBa_J69502/annotation1923086/range1923086/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_J69502/annotation1923086/range1923086/1
type: Range
persistent identity: range1923086
display id: range1923086
version: 1
direction: direction/reverse
top level: BBa_J69502
start: 58
end: 69
orientation: inline

BBa_R0051
type: SequenceAnnotation
persistent identity: annotation1923081
display id: annotation1923081
version: 1
top level: BBa_J69502
location: https://synbiohub.org/public/igem/BBa_J69502/annotation1923081/range1923081/1
component: BBa_R0051

https://synbiohub.org/public/igem/BBa_J69502/annotation1923081/range1923081/1
type: Range
persistent identity: range1923081
display id: range1923081
version: 1
direction: direction/forward
top level: BBa_J69502
start: 1
end: 49
orientation: inline

BBa_J06504
type: SequenceAnnotation
persistent identity: annotation1923089
display id: annotation1923089
version: 1
top level: BBa_J69502
location: https://synbiohub.org/public/igem/BBa_J69502/annotation1923089/range1923089/1
component: BBa_J06504

https://synbiohub.org/public/igem/BBa_J69502/annotation1923089/range1923089/1
type: Range
persistent identity: range1923089
display id: range1923089
version: 1
direction: direction/reverse
top level: BBa_J69502
start: 76
end: 789
orientation: inline

BBa_K1041000
type: ComponentDefinition
persistent identity: BBa K1041000
display id: BBa_K1041000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RFP Coding Device
creator: NRP UEA
created: 2013-08-07T11:00:00Z
modified: 2015-05-08T01:08:51Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1348_
m user id: 0
owner id: 17128
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: Mutagenesis was used to add a Nde1 site at the start of the RFP coding region of the biobrick BBa_J04450. This enables a restriction digest with Nde1 to be performed allowing the RFP coding region to be excised from the plasmid.
mutable notes: Nde1 site was added at the start of the RFP coding region.
mutable provenance: BBa_J04450
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: polya
sequence annotation: -10
sequence annotation: BBa_E1010
sequence annotation: conserved
sequence annotation: BBa_B0034
sequence annotation: T7 TE
sequence annotation: stem_loop
sequence annotation: BBa_B0012
sequence annotation: Help:Barcodes
sequence annotation: CAP binding site
sequence annotation: BBa_R0010
sequence annotation: stop
sequence annotation: -35
sequence annotation: start
sequence annotation: LacI binding site
sequence annotation: mrfp1
sequence annotation: Nde1 Restriction Site
sequence annotation: BBa_B0015
sequence annotation: BBa_B0010
sequence annotation: end of LacI coding region (inactive)
sequence: https://synbiohub.org/public/igem/BBa_K1041000_sequence/1

polya
type: SequenceAnnotation
persistent identity: annotation2331516
display id: annotation2331516
version: 1
top level: BBa_K1041000
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_K1041000/annotation2331516/range2331516/1

https://synbiohub.org/public/igem/BBa_K1041000/annotation2331516/range2331516/1
type: Range
persistent identity: range2331516
display id: range2331516
version: 1
direction: direction/forward
top level: BBa_K1041000
start: 1056
end: 1069
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2331504
display id: annotation2331504
version: 1
top level: BBa_K1041000
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1041000/annotation2331504/range2331504/1

https://synbiohub.org/public/igem/BBa_K1041000/annotation2331504/range2331504/1
type: Range
persistent identity: range2331504
display id: range2331504
version: 1
direction: direction/forward
top level: BBa_K1041000
start: 161
end: 166
orientation: inline

BBa_E1010
type: SequenceAnnotation
persistent identity: annotation2331511
display id: annotation2331511
version: 1
top level: BBa_K1041000
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1041000/annotation2331511/range2331511/1

https://synbiohub.org/public/igem/BBa_K1041000/annotation2331511/range2331511/1
type: Range
persistent identity: range2331511
display id: range2331511
version: 1
direction: direction/reverse
top level: BBa_K1041000
start: 227
end: 932
orientation: inline

conserved
type: SequenceAnnotation
persistent identity: annotation2331507
display id: annotation2331507
version: 1
top level: BBa_K1041000
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K1041000/annotation2331507/range2331507/1

https://synbiohub.org/public/igem/BBa_K1041000/annotation2331507/range2331507/1
type: Range
persistent identity: range2331507
display id: range2331507
version: 1
direction: direction/forward
top level: BBa_K1041000
start: 213
end: 216
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2331508
display id: annotation2331508
version: 1
top level: BBa_K1041000
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1041000/annotation2331508/range2331508/1

https://synbiohub.org/public/igem/BBa_K1041000/annotation2331508/range2331508/1
type: Range
persistent identity: range2331508
display id: range2331508
version: 1
direction: direction/reverse
top level: BBa_K1041000
start: 209
end: 220
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation2331515
display id: annotation2331515
version: 1
top level: BBa_K1041000
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1041000/annotation2331515/range2331515/1

https://synbiohub.org/public/igem/BBa_K1041000/annotation2331515/range2331515/1
type: Range
persistent identity: range2331515
display id: range2331515
version: 1
direction: direction/forward
top level: BBa_K1041000
start: 1036
end: 1055
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation2331513
display id: annotation2331513
version: 1
top level: BBa_K1041000
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1041000/annotation2331513/range2331513/1

https://synbiohub.org/public/igem/BBa_K1041000/annotation2331513/range2331513/1
type: Range
persistent identity: range2331513
display id: range2331513
version: 1
direction: direction/forward
top level: BBa_K1041000
start: 952
end: 995
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2331514
display id: annotation2331514
version: 1
top level: BBa_K1041000
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1041000/annotation2331514/range2331514/1

https://synbiohub.org/public/igem/BBa_K1041000/annotation2331514/range2331514/1
type: Range
persistent identity: range2331514
display id: range2331514
version: 1
direction: direction/forward
top level: BBa_K1041000
start: 1029
end: 1069
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2331510
display id: annotation2331510
version: 1
top level: BBa_K1041000
role: SO:0000807
role: feature/barcode
location: https://synbiohub.org/public/igem/BBa_K1041000/annotation2331510/range2331510/1

https://synbiohub.org/public/igem/BBa_K1041000/annotation2331510/range2331510/1
type: Range
persistent identity: range2331510
display id: range2331510
version: 1
direction: direction/forward
top level: BBa_K1041000
start: 908
end: 932
orientation: inline

CAP binding site
type: SequenceAnnotation
persistent identity: annotation2331502
display id: annotation2331502
version: 1
top level: BBa_K1041000
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1041000/annotation2331502/range2331502/1

https://synbiohub.org/public/igem/BBa_K1041000/annotation2331502/range2331502/1
type: Range
persistent identity: range2331502
display id: range2331502
version: 1
direction: direction/forward
top level: BBa_K1041000
start: 89
end: 126
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation2331500
display id: annotation2331500
version: 1
top level: BBa_K1041000
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1041000/annotation2331500/range2331500/1

https://synbiohub.org/public/igem/BBa_K1041000/annotation2331500/range2331500/1
type: Range
persistent identity: range2331500
display id: range2331500
version: 1
direction: direction/forward
top level: BBa_K1041000
start: 1
end: 200
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2331517
display id: annotation2331517
version: 1
top level: BBa_K1041000
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1041000/annotation2331517/range2331517/1

https://synbiohub.org/public/igem/BBa_K1041000/annotation2331517/range2331517/1
type: Range
persistent identity: range2331517
display id: range2331517
version: 1
direction: direction/forward
top level: BBa_K1041000
start: 1062
end: 1062
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2331503
display id: annotation2331503
version: 1
top level: BBa_K1041000
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1041000/annotation2331503/range2331503/1

https://synbiohub.org/public/igem/BBa_K1041000/annotation2331503/range2331503/1
type: Range
persistent identity: range2331503
display id: range2331503
version: 1
direction: direction/forward
top level: BBa_K1041000
start: 137
end: 142
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2331506
display id: annotation2331506
version: 1
top level: BBa_K1041000
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1041000/annotation2331506/range2331506/1

https://synbiohub.org/public/igem/BBa_K1041000/annotation2331506/range2331506/1
type: Range
persistent identity: range2331506
display id: range2331506
version: 1
direction: direction/forward
top level: BBa_K1041000
start: 173
end: 173
orientation: inline

LacI binding site
type: SequenceAnnotation
persistent identity: annotation2331505
display id: annotation2331505
version: 1
top level: BBa_K1041000
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1041000/annotation2331505/range2331505/1

https://synbiohub.org/public/igem/BBa_K1041000/annotation2331505/range2331505/1
type: Range
persistent identity: range2331505
display id: range2331505
version: 1
direction: direction/forward
top level: BBa_K1041000
start: 166
end: 200
orientation: inline

mrfp1
type: SequenceAnnotation
persistent identity: annotation2331509
display id: annotation2331509
version: 1
top level: BBa_K1041000
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1041000/annotation2331509/range2331509/1

https://synbiohub.org/public/igem/BBa_K1041000/annotation2331509/range2331509/1
type: Range
persistent identity: range2331509
display id: range2331509
version: 1
direction: direction/forward
top level: BBa_K1041000
start: 227
end: 901
orientation: inline

Nde1 Restriction Site
type: SequenceAnnotation
persistent identity: annotation2331523
display id: annotation2331523
version: 1
top level: BBa_K1041000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1041000/annotation2331523/range2331523/1

https://synbiohub.org/public/igem/BBa_K1041000/annotation2331523/range2331523/1
type: Range
persistent identity: range2331523
display id: range2331523
version: 1
direction: direction/forward
top level: BBa_K1041000
start: 224
end: 229
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2331518
display id: annotation2331518
version: 1
top level: BBa_K1041000
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1041000/annotation2331518/range2331518/1

https://synbiohub.org/public/igem/BBa_K1041000/annotation2331518/range2331518/1
type: Range
persistent identity: range2331518
display id: range2331518
version: 1
direction: direction/reverse
top level: BBa_K1041000
start: 941
end: 1069
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2331512
display id: annotation2331512
version: 1
top level: BBa_K1041000
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1041000/annotation2331512/range2331512/1

https://synbiohub.org/public/igem/BBa_K1041000/annotation2331512/range2331512/1
type: Range
persistent identity: range2331512
display id: range2331512
version: 1
direction: direction/forward
top level: BBa_K1041000
start: 941
end: 1020
orientation: inline

end of LacI coding region (inactive)
type: SequenceAnnotation
persistent identity: annotation2331501
display id: annotation2331501
version: 1
top level: BBa_K1041000
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1041000/annotation2331501/range2331501/1

https://synbiohub.org/public/igem/BBa_K1041000/annotation2331501/range2331501/1
type: Range
persistent identity: range2331501
display id: range2331501
version: 1
direction: direction/forward
top level: BBa_K1041000
start: 1
end: 88
orientation: inline

BBa_J5523
type: ComponentDefinition
persistent identity: BBa J5523
display id: BBa_J5523
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LacI -| mRFP
creator: Charles Yoon
created: 2006-10-30T12:00:00Z
is replaced by: https://synbiohub.org/public/igem/BBa_I13520/1
modified: 2015-08-31T03:22:59Z
discontinued: true
dominant: false
experience: experience/None
group u list: _82_
m user id: 0
owner id: 840
owning group id: 82
part status: Released HQ 2013
sample status: Discontinued
status: status/Deleted
bookmark: false
mutable description: mRFP repressed by LacI
mutable notes: none
mutable provenance: iGEM 2006 Registry
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_I0500
component: BBa_B0034
component: BBa_E1010
component: BBa_B0010
component: BBa_B0012
sequence annotation: BBa_B0034
sequence annotation: BBa_E1010
sequence annotation: BBa_I0500
sequence annotation: BBa_B0012
sequence annotation: BBa_B0010
sequence: https://synbiohub.org/public/igem/BBa_J5523_sequence/1

BBa_I0500
type: Component
persistent identity: component1908513
display id: component1908513
version: 1
top level: BBa_J5523
access: public
definition: https://synbiohub.org/public/igem/BBa_I0500/1

BBa_B0034
type: Component
persistent identity: component1908515
display id: component1908515
version: 1
top level: BBa_J5523
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_E1010
type: Component
persistent identity: component1908517
display id: component1908517
version: 1
top level: BBa_J5523
access: public
definition: https://synbiohub.org/public/igem/BBa_E1010/1

BBa_B0010
type: Component
persistent identity: component1908519
display id: component1908519
version: 1
top level: BBa_J5523
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
type: Component
persistent identity: component1908521
display id: component1908521
version: 1
top level: BBa_J5523
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1908515
display id: annotation1908515
version: 1
top level: BBa_J5523
location: https://synbiohub.org/public/igem/BBa_J5523/annotation1908515/range1908515/1
component: BBa_B0034

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type: Range
persistent identity: range1908515
display id: range1908515
version: 1
direction: direction/reverse
top level: BBa_J5523
start: 1219
end: 1230
orientation: inline

BBa_E1010
type: SequenceAnnotation
persistent identity: annotation1908517
display id: annotation1908517
version: 1
top level: BBa_J5523
location: https://synbiohub.org/public/igem/BBa_J5523/annotation1908517/range1908517/1
component: BBa_E1010

https://synbiohub.org/public/igem/BBa_J5523/annotation1908517/range1908517/1
type: Range
persistent identity: range1908517
display id: range1908517
version: 1
direction: direction/reverse
top level: BBa_J5523
start: 1237
end: 1917
orientation: inline

BBa_I0500
type: SequenceAnnotation
persistent identity: annotation1908513
display id: annotation1908513
version: 1
top level: BBa_J5523
location: https://synbiohub.org/public/igem/BBa_J5523/annotation1908513/range1908513/1
component: BBa_I0500

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type: Range
persistent identity: range1908513
display id: range1908513
version: 1
direction: direction/reverse
top level: BBa_J5523
start: 1
end: 1210
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1908521
display id: annotation1908521
version: 1
top level: BBa_J5523
location: https://synbiohub.org/public/igem/BBa_J5523/annotation1908521/range1908521/1
component: BBa_B0012

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type: Range
persistent identity: range1908521
display id: range1908521
version: 1
direction: direction/forward
top level: BBa_J5523
start: 2039
end: 2079
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1908519
display id: annotation1908519
version: 1
top level: BBa_J5523
location: https://synbiohub.org/public/igem/BBa_J5523/annotation1908519/range1908519/1
component: BBa_B0010

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type: Range
persistent identity: range1908519
display id: range1908519
version: 1
direction: direction/forward
top level: BBa_J5523
start: 1951
end: 2030
orientation: inline

BBa_J47863
type: ComponentDefinition
persistent identity: BBa J47863
display id: BBa_J47863
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pLacI + ECFP (LVA+)
creator: Jon Badalamenti
created: 2006-10-26T11:00:00Z
modified: 2015-08-31T04:08:51Z
discontinued: false
dominant: false
group u list: _72_40_
m user id: 0
owner id: 475
owning group id: 72
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: R0010.B0032.E0022.B0015
mutable notes: none.
mutable provenance: This part was constructed from parts available through the registry.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_R0010
component: BBa_B0012
component: BBa_E0022
component: BBa_B0010
component: BBa_B0032
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_E0022
sequence annotation: BBa_B0032
sequence annotation: BBa_R0010
sequence: https://synbiohub.org/public/igem/BBa_J47863_sequence/1

BBa_R0010
type: Component
persistent identity: component1907727
display id: component1907727
version: 1
top level: BBa_J47863
access: public
definition: https://synbiohub.org/public/igem/BBa_R0010/1

BBa_B0012
type: Component
persistent identity: component1907744
display id: component1907744
version: 1
top level: BBa_J47863
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_E0022
type: Component
persistent identity: component1907738
display id: component1907738
version: 1
top level: BBa_J47863
access: public
definition: https://synbiohub.org/public/igem/BBa_E0022/1

BBa_B0010
type: Component
persistent identity: component1907742
display id: component1907742
version: 1
top level: BBa_J47863
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0032
type: Component
persistent identity: component1907735
display id: component1907735
version: 1
top level: BBa_J47863
access: public
definition: https://synbiohub.org/public/igem/BBa_B0032/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1907742
display id: annotation1907742
version: 1
top level: BBa_J47863
location: https://synbiohub.org/public/igem/BBa_J47863/annotation1907742/range1907742/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_J47863/annotation1907742/range1907742/1
type: Range
persistent identity: range1907742
display id: range1907742
version: 1
direction: direction/forward
top level: BBa_J47863
start: 998
end: 1077
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1907744
display id: annotation1907744
version: 1
top level: BBa_J47863
location: https://synbiohub.org/public/igem/BBa_J47863/annotation1907744/range1907744/1
component: BBa_B0012

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type: Range
persistent identity: range1907744
display id: range1907744
version: 1
direction: direction/forward
top level: BBa_J47863
start: 1086
end: 1126
orientation: inline

BBa_E0022
type: SequenceAnnotation
persistent identity: annotation1907738
display id: annotation1907738
version: 1
top level: BBa_J47863
location: https://synbiohub.org/public/igem/BBa_J47863/annotation1907738/range1907738/1
component: BBa_E0022

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type: Range
persistent identity: range1907738
display id: range1907738
version: 1
direction: direction/forward
top level: BBa_J47863
start: 228
end: 989
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation1907735
display id: annotation1907735
version: 1
top level: BBa_J47863
location: https://synbiohub.org/public/igem/BBa_J47863/annotation1907735/range1907735/1
component: BBa_B0032

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type: Range
persistent identity: range1907735
display id: range1907735
version: 1
direction: direction/forward
top level: BBa_J47863
start: 209
end: 221
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation1907727
display id: annotation1907727
version: 1
top level: BBa_J47863
location: https://synbiohub.org/public/igem/BBa_J47863/annotation1907727/range1907727/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_J47863/annotation1907727/range1907727/1
type: Range
persistent identity: range1907727
display id: range1907727
version: 1
direction: direction/forward
top level: BBa_J47863
start: 1
end: 200
orientation: inline

BBa_K1399016
type: ComponentDefinition
persistent identity: BBa K1399016
display id: BBa_K1399016
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Plac-GFP(LAA)
creator: Anna Stikane
created: 2014-09-18T11:00:00Z
modified: 2015-05-08T01:10:16Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1777_
m user id: 0
owner id: 22477
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: Lactose/IPTG inducible promoter with GFP reporter tagged with AAV-ssrA degradation tag followed by terminator. The tagged GFP is actively degraded within cell, thus provides better temporal resolution of green fluorescence and promoter activity.
mutable notes: Part was assembled using BrickClip assembly (BBF RFC104) using other biobrick parts as templates. BrickClip assembly is a special case of more general Paperclip assembly method.[1]
mutable provenance: other biobricks
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_R0010
component: BBa_B0034
component: BBa_K1399006
component: BBa_B0015
sequence annotation: BBa_B0015
sequence annotation: BBa_B0034
sequence annotation: BBa_R0010
sequence annotation: BBa_K1399006
sequence: https://synbiohub.org/public/igem/BBa_K1399016_sequence/1

BBa_R0010
type: Component
persistent identity: component2384145
display id: component2384145
version: 1
top level: BBa_K1399016
access: public
definition: https://synbiohub.org/public/igem/BBa_R0010/1

BBa_B0034
type: Component
persistent identity: component2384153
display id: component2384153
version: 1
top level: BBa_K1399016
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K1399006
type: Component
persistent identity: component2384159
display id: component2384159
version: 1
top level: BBa_K1399016
access: public
definition: https://synbiohub.org/public/igem/BBa_K1399006/1

BBa_B0015
type: Component
persistent identity: component2384166
display id: component2384166
version: 1
top level: BBa_K1399016
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2384166
display id: annotation2384166
version: 1
top level: BBa_K1399016
location: https://synbiohub.org/public/igem/BBa_K1399016/annotation2384166/range2384166/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K1399016/annotation2384166/range2384166/1
type: Range
persistent identity: range2384166
display id: range2384166
version: 1
direction: direction/reverse
top level: BBa_K1399016
start: 988
end: 1116
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2384153
display id: annotation2384153
version: 1
top level: BBa_K1399016
location: https://synbiohub.org/public/igem/BBa_K1399016/annotation2384153/range2384153/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1399016/annotation2384153/range2384153/1
type: Range
persistent identity: range2384153
display id: range2384153
version: 1
direction: direction/reverse
top level: BBa_K1399016
start: 209
end: 220
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation2384145
display id: annotation2384145
version: 1
top level: BBa_K1399016
location: https://synbiohub.org/public/igem/BBa_K1399016/annotation2384145/range2384145/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_K1399016/annotation2384145/range2384145/1
type: Range
persistent identity: range2384145
display id: range2384145
version: 1
direction: direction/forward
top level: BBa_K1399016
start: 1
end: 200
orientation: inline

BBa_K1399006
type: SequenceAnnotation
persistent identity: annotation2384159
display id: annotation2384159
version: 1
top level: BBa_K1399016
location: https://synbiohub.org/public/igem/BBa_K1399016/annotation2384159/range2384159/1
component: BBa_K1399006

https://synbiohub.org/public/igem/BBa_K1399016/annotation2384159/range2384159/1
type: Range
persistent identity: range2384159
display id: range2384159
version: 1
direction: direction/reverse
top level: BBa_K1399016
start: 227
end: 979
orientation: inline

BBa_J22203
type: ComponentDefinition
persistent identity: BBa J22203
display id: BBa_J22203
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pLac-YFP
creator: Mukund Sugat Nilesh
created: 2007-06-20T11:00:00Z
modified: 2015-08-31T04:08:38Z
discontinued: false
dominant: false
experience: experience/None
group u list: _70_44_
m user id: 0
owner id: 439
owning group id: 70
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: a similar part is available in registry. we designed this as we faced some problems in YFP expression with the earlier part. we have tested this part and it is functional.
mutable notes: none
mutable provenance: registry
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_E0030
component: BBa_B0034
component: BBa_B0010
component: BBa_R0011
component: BBa_B0012
sequence annotation: BBa_B0012
sequence annotation: BBa_E0030
sequence annotation: BBa_B0010
sequence annotation: BBa_B0034
sequence annotation: BBa_R0011
sequence: https://synbiohub.org/public/igem/BBa_J22203_sequence/1

BBa_E0030
type: Component
persistent identity: component1935042
display id: component1935042
version: 1
top level: BBa_J22203
access: public
definition: https://synbiohub.org/public/igem/BBa_E0030/1

BBa_B0034
type: Component
persistent identity: component1935041
display id: component1935041
version: 1
top level: BBa_J22203
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0010
type: Component
persistent identity: component1935043
display id: component1935043
version: 1
top level: BBa_J22203
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_R0011
type: Component
persistent identity: component1935035
display id: component1935035
version: 1
top level: BBa_J22203
access: public
definition: https://synbiohub.org/public/igem/BBa_R0011/1

BBa_B0012
type: Component
persistent identity: component1935045
display id: component1935045
version: 1
top level: BBa_J22203
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1935045
display id: annotation1935045
version: 1
top level: BBa_J22203
location: https://synbiohub.org/public/igem/BBa_J22203/annotation1935045/range1935045/1
component: BBa_B0012

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type: Range
persistent identity: range1935045
display id: range1935045
version: 1
direction: direction/forward
top level: BBa_J22203
start: 901
end: 941
orientation: inline

BBa_E0030
type: SequenceAnnotation
persistent identity: annotation1935042
display id: annotation1935042
version: 1
top level: BBa_J22203
location: https://synbiohub.org/public/igem/BBa_J22203/annotation1935042/range1935042/1
component: BBa_E0030

https://synbiohub.org/public/igem/BBa_J22203/annotation1935042/range1935042/1
type: Range
persistent identity: range1935042
display id: range1935042
version: 1
direction: direction/reverse
top level: BBa_J22203
start: 82
end: 804
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1935043
display id: annotation1935043
version: 1
top level: BBa_J22203
location: https://synbiohub.org/public/igem/BBa_J22203/annotation1935043/range1935043/1
component: BBa_B0010

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type: Range
persistent identity: range1935043
display id: range1935043
version: 1
direction: direction/forward
top level: BBa_J22203
start: 813
end: 892
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1935041
display id: annotation1935041
version: 1
top level: BBa_J22203
location: https://synbiohub.org/public/igem/BBa_J22203/annotation1935041/range1935041/1
component: BBa_B0034

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type: Range
persistent identity: range1935041
display id: range1935041
version: 1
direction: direction/reverse
top level: BBa_J22203
start: 64
end: 75
orientation: inline

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation1935035
display id: annotation1935035
version: 1
top level: BBa_J22203
location: https://synbiohub.org/public/igem/BBa_J22203/annotation1935035/range1935035/1
component: BBa_R0011

https://synbiohub.org/public/igem/BBa_J22203/annotation1935035/range1935035/1
type: Range
persistent identity: range1935035
display id: range1935035
version: 1
direction: direction/forward
top level: BBa_J22203
start: 1
end: 54
orientation: inline

BBa_M30000
type: ComponentDefinition
persistent identity: BBa M30000
display id: BBa_M30000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: OmpR:lacZ reporter
creator: natalie kuldell
created: 2006-01-13T12:00:00Z
modified: 2015-05-08T01:13:58Z
discontinued: false
dominant: false
group u list: _45_1_
m user id: 0
owner id: 314
owning group id: 1
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Positively regulated, OmpR-controlled promoter from OmpC gene (R0082) directing transcription of lacZ reporter device (I2012).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_I2012
component: BBa_R0082
sequence annotation: BBa_R0082
sequence annotation: BBa_I2012
sequence: https://synbiohub.org/public/igem/BBa_M30000_sequence/1

BBa_I2012
type: Component
persistent identity: component2221866
display id: component2221866
version: 1
top level: BBa_M30000
access: public
definition: https://synbiohub.org/public/igem/BBa_I2012/1

BBa_R0082
type: Component
persistent identity: component2221845
display id: component2221845
version: 1
top level: BBa_M30000
access: public
definition: https://synbiohub.org/public/igem/BBa_R0082/1

BBa_R0082
type: SequenceAnnotation
persistent identity: annotation2221845
display id: annotation2221845
version: 1
top level: BBa_M30000
location: https://synbiohub.org/public/igem/BBa_M30000/annotation2221845/range2221845/1
component: BBa_R0082

https://synbiohub.org/public/igem/BBa_M30000/annotation2221845/range2221845/1
type: Range
persistent identity: range2221845
display id: range2221845
version: 1
direction: direction/reverse
top level: BBa_M30000
start: 1
end: 108
orientation: inline

BBa_I2012
type: SequenceAnnotation
persistent identity: annotation2221866
display id: annotation2221866
version: 1
top level: BBa_M30000
location: https://synbiohub.org/public/igem/BBa_M30000/annotation2221866/range2221866/1
component: BBa_I2012

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type: Range
persistent identity: range2221866
display id: range2221866
version: 1
direction: direction/reverse
top level: BBa_M30000
start: 117
end: 624
orientation: inline

BBa_I13578
type: ComponentDefinition
persistent identity: BBa I13578
display id: BBa_I13578
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ins + RFP (no promoter)
creator: Jason Kelly
created: 2007-06-13T11:00:00Z
is replaced by: https://synbiohub.org/public/igem/BBa_I13528/1
modified: 2015-08-31T04:07:35Z
discontinued: true
dominant: false
experience: experience/None
group u list: _11_84_
m user id: 0
owner id: 2
owning group id: 84
part status: Released HQ 2013
sample status: Discontinued
status: status/Deleted
bookmark: false
mutable description: Test construct for Screening Plasmid insertion site (No Promoter)
mutable notes: .
mutable provenance: .
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_B0010
component: BBa_B0012
component: BBa_I13457
component: BBa_E1010
component: BBa_B0034
sequence annotation: BBa_E1010
sequence annotation: BBa_B0034
sequence annotation: BBa_B0010
sequence annotation: BBa_I13457
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_I13578_sequence/1

BBa_B0010
type: Component
persistent identity: component1934637
display id: component1934637
version: 1
top level: BBa_I13578
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
type: Component
persistent identity: component1934639
display id: component1934639
version: 1
top level: BBa_I13578
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_I13457
type: Component
persistent identity: component1934631
display id: component1934631
version: 1
top level: BBa_I13578
access: public
definition: https://synbiohub.org/public/igem/BBa_I13457/1

BBa_E1010
type: Component
persistent identity: component1934635
display id: component1934635
version: 1
top level: BBa_I13578
access: public
definition: https://synbiohub.org/public/igem/BBa_E1010/1

BBa_B0034
type: Component
persistent identity: component1934633
display id: component1934633
version: 1
top level: BBa_I13578
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_E1010
type: SequenceAnnotation
persistent identity: annotation1934635
display id: annotation1934635
version: 1
top level: BBa_I13578
location: https://synbiohub.org/public/igem/BBa_I13578/annotation1934635/range1934635/1
component: BBa_E1010

https://synbiohub.org/public/igem/BBa_I13578/annotation1934635/range1934635/1
type: Range
persistent identity: range1934635
display id: range1934635
version: 1
direction: direction/reverse
top level: BBa_I13578
start: 138
end: 818
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1934633
display id: annotation1934633
version: 1
top level: BBa_I13578
location: https://synbiohub.org/public/igem/BBa_I13578/annotation1934633/range1934633/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I13578/annotation1934633/range1934633/1
type: Range
persistent identity: range1934633
display id: range1934633
version: 1
direction: direction/reverse
top level: BBa_I13578
start: 120
end: 131
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1934637
display id: annotation1934637
version: 1
top level: BBa_I13578
location: https://synbiohub.org/public/igem/BBa_I13578/annotation1934637/range1934637/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I13578/annotation1934637/range1934637/1
type: Range
persistent identity: range1934637
display id: range1934637
version: 1
direction: direction/forward
top level: BBa_I13578
start: 852
end: 931
orientation: inline

BBa_I13457
type: SequenceAnnotation
persistent identity: annotation1934631
display id: annotation1934631
version: 1
top level: BBa_I13578
location: https://synbiohub.org/public/igem/BBa_I13578/annotation1934631/range1934631/1
component: BBa_I13457

https://synbiohub.org/public/igem/BBa_I13578/annotation1934631/range1934631/1
type: Range
persistent identity: range1934631
display id: range1934631
version: 1
direction: direction/reverse
top level: BBa_I13578
start: 1
end: 111
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1934639
display id: annotation1934639
version: 1
top level: BBa_I13578
location: https://synbiohub.org/public/igem/BBa_I13578/annotation1934639/range1934639/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I13578/annotation1934639/range1934639/1
type: Range
persistent identity: range1934639
display id: range1934639
version: 1
direction: direction/forward
top level: BBa_I13578
start: 940
end: 980
orientation: inline

BBa_J06003
type: ComponentDefinition
persistent identity: BBa J06003
display id: BBa_J06003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: tetR repressible ECFP reporter
creator: sash31
created: 2005-06-12T11:00:00Z
modified: 2015-08-31T04:08:16Z
discontinued: false
dominant: false
experience: experience/None
group u list: _20_
m user id: 0
owner id: 339
owning group id: 20
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: In absence of tetR, ECFP is expressed
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_R0040
component: BBa_B0010
component: BBa_B0034
component: BBa_B0012
component: BBa_E0022
sequence annotation: BBa_B0034
sequence annotation: BBa_E0022
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_R0040
sequence: https://synbiohub.org/public/igem/BBa_J06003_sequence/1

BBa_R0040
type: Component
persistent identity: component1513090
display id: component1513090
version: 1
top level: BBa_J06003
access: public
definition: https://synbiohub.org/public/igem/BBa_R0040/1

BBa_B0010
type: Component
persistent identity: component1513118
display id: component1513118
version: 1
top level: BBa_J06003
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0034
type: Component
persistent identity: component1513098
display id: component1513098
version: 1
top level: BBa_J06003
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0012
type: Component
persistent identity: component1513128
display id: component1513128
version: 1
top level: BBa_J06003
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_E0022
type: Component
persistent identity: component1513110
display id: component1513110
version: 1
top level: BBa_J06003
access: public
definition: https://synbiohub.org/public/igem/BBa_E0022/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1513098
display id: annotation1513098
version: 1
top level: BBa_J06003
location: https://synbiohub.org/public/igem/BBa_J06003/annotation1513098/range1513098/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_J06003/annotation1513098/range1513098/1
type: Range
persistent identity: range1513098
display id: range1513098
version: 1
direction: direction/forward
top level: BBa_J06003
start: 63
end: 74
orientation: inline

BBa_E0022
type: SequenceAnnotation
persistent identity: annotation1513110
display id: annotation1513110
version: 1
top level: BBa_J06003
location: https://synbiohub.org/public/igem/BBa_J06003/annotation1513110/range1513110/1
component: BBa_E0022

https://synbiohub.org/public/igem/BBa_J06003/annotation1513110/range1513110/1
type: Range
persistent identity: range1513110
display id: range1513110
version: 1
direction: direction/forward
top level: BBa_J06003
start: 81
end: 842
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1513118
display id: annotation1513118
version: 1
top level: BBa_J06003
location: https://synbiohub.org/public/igem/BBa_J06003/annotation1513118/range1513118/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_J06003/annotation1513118/range1513118/1
type: Range
persistent identity: range1513118
display id: range1513118
version: 1
direction: direction/forward
top level: BBa_J06003
start: 851
end: 930
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1513128
display id: annotation1513128
version: 1
top level: BBa_J06003
location: https://synbiohub.org/public/igem/BBa_J06003/annotation1513128/range1513128/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_J06003/annotation1513128/range1513128/1
type: Range
persistent identity: range1513128
display id: range1513128
version: 1
direction: direction/forward
top level: BBa_J06003
start: 939
end: 979
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1513090
display id: annotation1513090
version: 1
top level: BBa_J06003
location: https://synbiohub.org/public/igem/BBa_J06003/annotation1513090/range1513090/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_J06003/annotation1513090/range1513090/1
type: Range
persistent identity: range1513090
display id: range1513090
version: 1
direction: direction/forward
top level: BBa_J06003
start: 1
end: 54
orientation: inline

BBa_I13602
type: ComponentDefinition
persistent identity: BBa I13602
display id: BBa_I13602
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Tet operator with CFP reporter (with LVA tag) [R/Tc+]
creator: Christopher Batten, Victoria Chou, Kenneth Nesmith
created: 2004-07-15T11:00:00Z
modified: 2015-08-31T04:07:36Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _6_
m user id: 0
owner id: 101
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_E0022
component: BBa_B0010
component: BBa_B0012
component: BBa_R0040
component: BBa_B0034
sequence annotation: BBa_B0012
sequence annotation: BBa_R0040
sequence annotation: BBa_B0010
sequence annotation: BBa_B0034
sequence annotation: BBa_E0022
sequence: https://synbiohub.org/public/igem/BBa_I13602_sequence/1

BBa_E0022
type: Component
persistent identity: component947274
display id: component947274
version: 1
top level: BBa_I13602
access: public
definition: https://synbiohub.org/public/igem/BBa_E0022/1

BBa_B0010
type: Component
persistent identity: component947282
display id: component947282
version: 1
top level: BBa_I13602
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
type: Component
persistent identity: component947292
display id: component947292
version: 1
top level: BBa_I13602
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_R0040
type: Component
persistent identity: component947254
display id: component947254
version: 1
top level: BBa_I13602
access: public
definition: https://synbiohub.org/public/igem/BBa_R0040/1

BBa_B0034
type: Component
persistent identity: component947262
display id: component947262
version: 1
top level: BBa_I13602
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation947292
display id: annotation947292
version: 1
top level: BBa_I13602
location: https://synbiohub.org/public/igem/BBa_I13602/annotation947292/range947292/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I13602/annotation947292/range947292/1
type: Range
persistent identity: range947292
display id: range947292
version: 1
direction: direction/forward
top level: BBa_I13602
start: 939
end: 979
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation947254
display id: annotation947254
version: 1
top level: BBa_I13602
location: https://synbiohub.org/public/igem/BBa_I13602/annotation947254/range947254/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_I13602/annotation947254/range947254/1
type: Range
persistent identity: range947254
display id: range947254
version: 1
direction: direction/forward
top level: BBa_I13602
start: 1
end: 54
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation947282
display id: annotation947282
version: 1
top level: BBa_I13602
location: https://synbiohub.org/public/igem/BBa_I13602/annotation947282/range947282/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I13602/annotation947282/range947282/1
type: Range
persistent identity: range947282
display id: range947282
version: 1
direction: direction/forward
top level: BBa_I13602
start: 851
end: 930
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation947262
display id: annotation947262
version: 1
top level: BBa_I13602
location: https://synbiohub.org/public/igem/BBa_I13602/annotation947262/range947262/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I13602/annotation947262/range947262/1
type: Range
persistent identity: range947262
display id: range947262
version: 1
direction: direction/forward
top level: BBa_I13602
start: 63
end: 74
orientation: inline

BBa_E0022
type: SequenceAnnotation
persistent identity: annotation947274
display id: annotation947274
version: 1
top level: BBa_I13602
location: https://synbiohub.org/public/igem/BBa_I13602/annotation947274/range947274/1
component: BBa_E0022

https://synbiohub.org/public/igem/BBa_I13602/annotation947274/range947274/1
type: Range
persistent identity: range947274
display id: range947274
version: 1
direction: direction/forward
top level: BBa_I13602
start: 81
end: 842
orientation: inline

BBa_J58101
type: ComponentDefinition
persistent identity: BBa J58101
display id: BBa_J58101
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter activated synergistically by cI and CRP with the reporter RFP
creator: Valencia iGEM 2006
created: 2006-10-25T11:00:00Z
modified: 2015-08-31T03:14:48Z
discontinued: false
dominant: false
experience: experience/None
group u list: _83_
m user id: 0
owner id: 1100
owning group id: 83
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: A promoter activated synergistically by cI and CRP proteins (part ref. BBa_J58011) with a generator of the reporter RFP (part ref. BBa_I13507). This generator uses the following parts: BBa_B0034 (RBS), BBa_E1010 (mRFP1), and BBa_B0015 (Terminator). With this construction, we obtain an AND gate, with RFP as output, and cI and CRP as inputs.
mutable notes: Notice that in the promoter sequence the XbaI restricction site is present. Therefore, we have to digest this part upstream only with EcoRI.
mutable provenance: To make this composite part we assemby two parts from the Registry (a promoter and a generator).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_J58100
component: BBa_I13507
sequence annotation: BBa_J58100
sequence annotation: BBa_I13507
sequence: https://synbiohub.org/public/igem/BBa_J58101_sequence/1

BBa_J58100
type: Component
persistent identity: component2252040
display id: component2252040
version: 1
top level: BBa_J58101
access: public
definition: https://synbiohub.org/public/igem/BBa_J58100/1

BBa_I13507
type: Component
persistent identity: component2252052
display id: component2252052
version: 1
top level: BBa_J58101
access: public
definition: https://synbiohub.org/public/igem/BBa_I13507/1

BBa_J58100
type: SequenceAnnotation
persistent identity: annotation2252040
display id: annotation2252040
version: 1
top level: BBa_J58101
location: https://synbiohub.org/public/igem/BBa_J58101/annotation2252040/range2252040/1
component: BBa_J58100

https://synbiohub.org/public/igem/BBa_J58101/annotation2252040/range2252040/1
type: Range
persistent identity: range2252040
display id: range2252040
version: 1
direction: direction/reverse
top level: BBa_J58101
start: 1
end: 106
orientation: inline

BBa_I13507
type: SequenceAnnotation
persistent identity: annotation2252052
display id: annotation2252052
version: 1
top level: BBa_J58101
location: https://synbiohub.org/public/igem/BBa_J58101/annotation2252052/range2252052/1
component: BBa_I13507

https://synbiohub.org/public/igem/BBa_J58101/annotation2252052/range2252052/1
type: Range
persistent identity: range2252052
display id: range2252052
version: 1
direction: direction/reverse
top level: BBa_J58101
start: 115
end: 975
orientation: inline

mRFP
type: ComponentDefinition
persistent identity: BBa J5526
display id: BBa_J5526
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LacI -| mRFP
creator: Charles Yoon
created: 2006-10-30T12:00:00Z
modified: 2015-08-31T03:22:59Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _82_
m user id: 0
owner id: 840
owning group id: 82
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: In the presence of LacI, the pLac promoter will be tightly repressed and not produce mRFP
mutable notes: none
mutable provenance: iGEM 2006 Registry
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_R0011
component: BBa_I13507
sequence annotation: BBa_R0011
sequence annotation: BBa_I13507
sequence: https://synbiohub.org/public/igem/BBa_J5526_sequence/1

BBa_R0011
type: Component
persistent identity: component2252262
display id: component2252262
version: 1
top level: mRFP
access: public
definition: https://synbiohub.org/public/igem/BBa_R0011/1

BBa_I13507
type: Component
persistent identity: component2252278
display id: component2252278
version: 1
top level: mRFP
access: public
definition: https://synbiohub.org/public/igem/BBa_I13507/1

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation2252262
display id: annotation2252262
version: 1
top level: mRFP
location: https://synbiohub.org/public/igem/BBa_J5526/annotation2252262/range2252262/1
component: BBa_R0011

https://synbiohub.org/public/igem/BBa_J5526/annotation2252262/range2252262/1
type: Range
persistent identity: range2252262
display id: range2252262
version: 1
direction: direction/forward
top level: mRFP
start: 1
end: 54
orientation: inline

BBa_I13507
type: SequenceAnnotation
persistent identity: annotation2252278
display id: annotation2252278
version: 1
top level: mRFP
location: https://synbiohub.org/public/igem/BBa_J5526/annotation2252278/range2252278/1
component: BBa_I13507

https://synbiohub.org/public/igem/BBa_J5526/annotation2252278/range2252278/1
type: Range
persistent identity: range2252278
display id: range2252278
version: 1
direction: direction/reverse
top level: mRFP
start: 64
end: 924
orientation: inline

BBa_K1399018
type: ComponentDefinition
persistent identity: BBa K1399018
display id: BBa_K1399018
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Plac-GFP(DAS)
creator: Anna Stikane
created: 2014-09-18T11:00:00Z
modified: 2015-05-08T01:10:16Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1777_
m user id: 0
owner id: 22477
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: Lactose/IPTG inducible promoter with GFP reporter tagged with DAS-ssrA degradation tag followed by terminator. The tagged GFP is actively degraded within cell, thus provides better temporal resolution of green fluorescence and promoter activity. Presence of SspB is crucial for effective protein degradation.
mutable notes: Part was assembled using BrickClip assembly (BBF RFC104) using other biobrick parts as templates. BrickClip assembly is a special case of more general Paperclip assembly method.[1]
mutable provenance: other biobricks
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_R0010
component: BBa_B0015
component: BBa_K1399008
component: BBa_B0034
sequence annotation: BBa_R0010
sequence annotation: BBa_B0034
sequence annotation: BBa_K1399008
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_K1399018_sequence/1

BBa_R0010
type: Component
persistent identity: component2384197
display id: component2384197
version: 1
top level: BBa_K1399018
access: public
definition: https://synbiohub.org/public/igem/BBa_R0010/1

BBa_B0015
type: Component
persistent identity: component2384218
display id: component2384218
version: 1
top level: BBa_K1399018
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_K1399008
type: Component
persistent identity: component2384211
display id: component2384211
version: 1
top level: BBa_K1399018
access: public
definition: https://synbiohub.org/public/igem/BBa_K1399008/1

BBa_B0034
type: Component
persistent identity: component2384205
display id: component2384205
version: 1
top level: BBa_K1399018
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation2384197
display id: annotation2384197
version: 1
top level: BBa_K1399018
location: https://synbiohub.org/public/igem/BBa_K1399018/annotation2384197/range2384197/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_K1399018/annotation2384197/range2384197/1
type: Range
persistent identity: range2384197
display id: range2384197
version: 1
direction: direction/forward
top level: BBa_K1399018
start: 1
end: 200
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2384205
display id: annotation2384205
version: 1
top level: BBa_K1399018
location: https://synbiohub.org/public/igem/BBa_K1399018/annotation2384205/range2384205/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1399018/annotation2384205/range2384205/1
type: Range
persistent identity: range2384205
display id: range2384205
version: 1
direction: direction/reverse
top level: BBa_K1399018
start: 209
end: 220
orientation: inline

BBa_K1399008
type: SequenceAnnotation
persistent identity: annotation2384211
display id: annotation2384211
version: 1
top level: BBa_K1399018
location: https://synbiohub.org/public/igem/BBa_K1399018/annotation2384211/range2384211/1
component: BBa_K1399008

https://synbiohub.org/public/igem/BBa_K1399018/annotation2384211/range2384211/1
type: Range
persistent identity: range2384211
display id: range2384211
version: 1
direction: direction/reverse
top level: BBa_K1399018
start: 227
end: 979
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2384218
display id: annotation2384218
version: 1
top level: BBa_K1399018
location: https://synbiohub.org/public/igem/BBa_K1399018/annotation2384218/range2384218/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K1399018/annotation2384218/range2384218/1
type: Range
persistent identity: range2384218
display id: range2384218
version: 1
direction: direction/reverse
top level: BBa_K1399018
start: 988
end: 1116
orientation: inline

BBa_J07066
type: ComponentDefinition
persistent identity: BBa J07066
display id: BBa_J07066
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: FecA Promoter-RFP test construct
creator: Annie Vo
created: 2005-08-21T11:00:00Z
modified: 2015-08-31T04:08:20Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _21_
m user id: 0
owner id: 353
owning group id: 21
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: Test construct for FecA Promoter. Includes FecA Promoter (J07019) and RFG (I13507).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_B0010
component: BBa_J07019
component: BBa_B0012
component: BBa_E1010
component: BBa_B0034
sequence annotation: BBa_B0034
sequence annotation: BBa_B0010
sequence annotation: BBa_E1010
sequence annotation: BBa_J07019
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_J07066_sequence/1

BBa_B0010
type: Component
persistent identity: component1664684
display id: component1664684
version: 1
top level: BBa_J07066
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_J07019
type: Component
persistent identity: component1664666
display id: component1664666
version: 1
top level: BBa_J07066
access: public
definition: https://synbiohub.org/public/igem/BBa_J07019/1

BBa_B0012
type: Component
persistent identity: component1664694
display id: component1664694
version: 1
top level: BBa_J07066
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_E1010
type: Component
persistent identity: component1664678
display id: component1664678
version: 1
top level: BBa_J07066
access: public
definition: https://synbiohub.org/public/igem/BBa_E1010/1

BBa_B0034
type: Component
persistent identity: component1664671
display id: component1664671
version: 1
top level: BBa_J07066
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1664671
display id: annotation1664671
version: 1
top level: BBa_J07066
location: https://synbiohub.org/public/igem/BBa_J07066/annotation1664671/range1664671/1
component: BBa_B0034

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type: Range
persistent identity: range1664671
display id: range1664671
version: 1
direction: direction/forward
top level: BBa_J07066
start: 95
end: 106
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1664684
display id: annotation1664684
version: 1
top level: BBa_J07066
location: https://synbiohub.org/public/igem/BBa_J07066/annotation1664684/range1664684/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_J07066/annotation1664684/range1664684/1
type: Range
persistent identity: range1664684
display id: range1664684
version: 1
direction: direction/forward
top level: BBa_J07066
start: 827
end: 906
orientation: inline

BBa_E1010
type: SequenceAnnotation
persistent identity: annotation1664678
display id: annotation1664678
version: 1
top level: BBa_J07066
location: https://synbiohub.org/public/igem/BBa_J07066/annotation1664678/range1664678/1
component: BBa_E1010

https://synbiohub.org/public/igem/BBa_J07066/annotation1664678/range1664678/1
type: Range
persistent identity: range1664678
display id: range1664678
version: 1
direction: direction/forward
top level: BBa_J07066
start: 113
end: 793
orientation: inline

BBa_J07019
type: SequenceAnnotation
persistent identity: annotation1664666
display id: annotation1664666
version: 1
top level: BBa_J07066
location: https://synbiohub.org/public/igem/BBa_J07066/annotation1664666/range1664666/1
component: BBa_J07019

https://synbiohub.org/public/igem/BBa_J07066/annotation1664666/range1664666/1
type: Range
persistent identity: range1664666
display id: range1664666
version: 1
direction: direction/forward
top level: BBa_J07066
start: 1
end: 86
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1664694
display id: annotation1664694
version: 1
top level: BBa_J07066
location: https://synbiohub.org/public/igem/BBa_J07066/annotation1664694/range1664694/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_J07066/annotation1664694/range1664694/1
type: Range
persistent identity: range1664694
display id: range1664694
version: 1
direction: direction/forward
top level: BBa_J07066
start: 915
end: 955
orientation: inline

BBa_J21001
type: ComponentDefinition
persistent identity: BBa J21001
display id: BBa_J21001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LuxR controlled RFP
creator: Josh Kittleson
created: 2006-06-26T11:00:00Z
modified: 2015-08-31T04:08:37Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _55_
m user id: 0
owner id: 947
owning group id: 55
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: LuxR controlled operator in front of POPS-->RFP part.
mutable notes: n/a
mutable provenance: n/a
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_J06504
component: BBa_B0012
component: BBa_B0034
component: BBa_B0010
component: BBa_R0062
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_J06504
sequence annotation: BBa_B0034
sequence annotation: BBa_R0062
sequence: https://synbiohub.org/public/igem/BBa_J21001_sequence/1

BBa_J06504
type: Component
persistent identity: component1882391
display id: component1882391
version: 1
top level: BBa_J21001
access: public
definition: https://synbiohub.org/public/igem/BBa_J06504/1

BBa_B0012
type: Component
persistent identity: component1882394
display id: component1882394
version: 1
top level: BBa_J21001
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0034
type: Component
persistent identity: component1882388
display id: component1882388
version: 1
top level: BBa_J21001
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0010
type: Component
persistent identity: component1882392
display id: component1882392
version: 1
top level: BBa_J21001
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_R0062
type: Component
persistent identity: component1882383
display id: component1882383
version: 1
top level: BBa_J21001
access: public
definition: https://synbiohub.org/public/igem/BBa_R0062/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1882392
display id: annotation1882392
version: 1
top level: BBa_J21001
location: https://synbiohub.org/public/igem/BBa_J21001/annotation1882392/range1882392/1
component: BBa_B0010

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type: Range
persistent identity: range1882392
display id: range1882392
version: 1
direction: direction/forward
top level: BBa_J21001
start: 804
end: 883
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1882394
display id: annotation1882394
version: 1
top level: BBa_J21001
location: https://synbiohub.org/public/igem/BBa_J21001/annotation1882394/range1882394/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_J21001/annotation1882394/range1882394/1
type: Range
persistent identity: range1882394
display id: range1882394
version: 1
direction: direction/forward
top level: BBa_J21001
start: 892
end: 932
orientation: inline

BBa_J06504
type: SequenceAnnotation
persistent identity: annotation1882391
display id: annotation1882391
version: 1
top level: BBa_J21001
location: https://synbiohub.org/public/igem/BBa_J21001/annotation1882391/range1882391/1
component: BBa_J06504

https://synbiohub.org/public/igem/BBa_J21001/annotation1882391/range1882391/1
type: Range
persistent identity: range1882391
display id: range1882391
version: 1
direction: direction/reverse
top level: BBa_J21001
start: 82
end: 795
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1882388
display id: annotation1882388
version: 1
top level: BBa_J21001
location: https://synbiohub.org/public/igem/BBa_J21001/annotation1882388/range1882388/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_J21001/annotation1882388/range1882388/1
type: Range
persistent identity: range1882388
display id: range1882388
version: 1
direction: direction/reverse
top level: BBa_J21001
start: 64
end: 75
orientation: inline

BBa_R0062
type: SequenceAnnotation
persistent identity: annotation1882383
display id: annotation1882383
version: 1
top level: BBa_J21001
location: https://synbiohub.org/public/igem/BBa_J21001/annotation1882383/range1882383/1
component: BBa_R0062

https://synbiohub.org/public/igem/BBa_J21001/annotation1882383/range1882383/1
type: Range
persistent identity: range1882383
display id: range1882383
version: 1
direction: direction/forward
top level: BBa_J21001
start: 1
end: 55
orientation: inline

BBa_J09265
type: ComponentDefinition
persistent identity: BBa J09265
display id: BBa_J09265
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pLuxR + GFP (AAV+)
creator: Jon Badalamenti
created: 2006-10-26T11:00:00Z
modified: 2015-08-31T04:08:21Z
discontinued: false
dominant: false
group u list: _72_40_
m user id: 0
owner id: 475
owning group id: 72
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: R0062.B0034.E0044.B0015
mutable notes: none.
mutable provenance: This part was constructed from parts available through the registry.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_B0012
component: BBa_B0034
component: BBa_B0010
component: BBa_E0044
component: BBa_R0062
sequence annotation: BBa_R0062
sequence annotation: BBa_E0044
sequence annotation: BBa_B0010
sequence annotation: BBa_B0034
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_J09265_sequence/1

BBa_B0012
type: Component
persistent identity: component1907387
display id: component1907387
version: 1
top level: BBa_J09265
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0034
type: Component
persistent identity: component1907383
display id: component1907383
version: 1
top level: BBa_J09265
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0010
type: Component
persistent identity: component1907385
display id: component1907385
version: 1
top level: BBa_J09265
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_E0044
type: Component
persistent identity: component1907384
display id: component1907384
version: 1
top level: BBa_J09265
access: public
definition: https://synbiohub.org/public/igem/BBa_E0044/1

BBa_R0062
type: Component
persistent identity: component1907378
display id: component1907378
version: 1
top level: BBa_J09265
access: public
definition: https://synbiohub.org/public/igem/BBa_R0062/1

BBa_R0062
type: SequenceAnnotation
persistent identity: annotation1907378
display id: annotation1907378
version: 1
top level: BBa_J09265
location: https://synbiohub.org/public/igem/BBa_J09265/annotation1907378/range1907378/1
component: BBa_R0062

https://synbiohub.org/public/igem/BBa_J09265/annotation1907378/range1907378/1
type: Range
persistent identity: range1907378
display id: range1907378
version: 1
direction: direction/forward
top level: BBa_J09265
start: 1
end: 55
orientation: inline

BBa_E0044
type: SequenceAnnotation
persistent identity: annotation1907384
display id: annotation1907384
version: 1
top level: BBa_J09265
location: https://synbiohub.org/public/igem/BBa_J09265/annotation1907384/range1907384/1
component: BBa_E0044

https://synbiohub.org/public/igem/BBa_J09265/annotation1907384/range1907384/1
type: Range
persistent identity: range1907384
display id: range1907384
version: 1
direction: direction/reverse
top level: BBa_J09265
start: 82
end: 840
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1907385
display id: annotation1907385
version: 1
top level: BBa_J09265
location: https://synbiohub.org/public/igem/BBa_J09265/annotation1907385/range1907385/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_J09265/annotation1907385/range1907385/1
type: Range
persistent identity: range1907385
display id: range1907385
version: 1
direction: direction/forward
top level: BBa_J09265
start: 849
end: 928
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1907383
display id: annotation1907383
version: 1
top level: BBa_J09265
location: https://synbiohub.org/public/igem/BBa_J09265/annotation1907383/range1907383/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_J09265/annotation1907383/range1907383/1
type: Range
persistent identity: range1907383
display id: range1907383
version: 1
direction: direction/reverse
top level: BBa_J09265
start: 64
end: 75
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1907387
display id: annotation1907387
version: 1
top level: BBa_J09265
location: https://synbiohub.org/public/igem/BBa_J09265/annotation1907387/range1907387/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_J09265/annotation1907387/range1907387/1
type: Range
persistent identity: range1907387
display id: range1907387
version: 1
direction: direction/forward
top level: BBa_J09265
start: 937
end: 977
orientation: inline

BBa_E70101
type: ComponentDefinition
persistent identity: BBa E70101
display id: BBa_E70101
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: GFP expression system that uses dedicated translation
creator: Barry Canton
created: 2006-01-26T12:00:00Z
modified: 2015-08-31T04:07:27Z
discontinued: false
dominant: false
group u list: _11_6_
m user id: 0
owner id: 135
owning group id: 6
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: This expression device requires the presence of dedicated ribosomes to produce GFP in E. coli. It will be tested in conjuction with E70101 which requires both dedicated ribosomes and dedicated transcription.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_B0037
component: BBa_E0040
component: BBa_R0040
component: BBa_B0010
component: BBa_B0012
sequence annotation: BBa_E0040
sequence annotation: BBa_B0037
sequence annotation: BBa_R0040
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_E70101_sequence/1

BBa_B0037
type: Component
persistent identity: component1791795
display id: component1791795
version: 1
top level: BBa_E70101
access: public
definition: https://synbiohub.org/public/igem/BBa_B0037/1

BBa_E0040
type: Component
persistent identity: component1791797
display id: component1791797
version: 1
top level: BBa_E70101
access: public
definition: https://synbiohub.org/public/igem/BBa_E0040/1

BBa_R0040
type: Component
persistent identity: component1791790
display id: component1791790
version: 1
top level: BBa_E70101
access: public
definition: https://synbiohub.org/public/igem/BBa_R0040/1

BBa_B0010
type: Component
persistent identity: component1791802
display id: component1791802
version: 1
top level: BBa_E70101
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
type: Component
persistent identity: component1791812
display id: component1791812
version: 1
top level: BBa_E70101
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation1791797
display id: annotation1791797
version: 1
top level: BBa_E70101
location: https://synbiohub.org/public/igem/BBa_E70101/annotation1791797/range1791797/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_E70101/annotation1791797/range1791797/1
type: Range
persistent identity: range1791797
display id: range1791797
version: 1
direction: direction/reverse
top level: BBa_E70101
start: 79
end: 798
orientation: inline

BBa_B0037
type: SequenceAnnotation
persistent identity: annotation1791795
display id: annotation1791795
version: 1
top level: BBa_E70101
location: https://synbiohub.org/public/igem/BBa_E70101/annotation1791795/range1791795/1
component: BBa_B0037

https://synbiohub.org/public/igem/BBa_E70101/annotation1791795/range1791795/1
type: Range
persistent identity: range1791795
display id: range1791795
version: 1
direction: direction/reverse
top level: BBa_E70101
start: 63
end: 72
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1791790
display id: annotation1791790
version: 1
top level: BBa_E70101
location: https://synbiohub.org/public/igem/BBa_E70101/annotation1791790/range1791790/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_E70101/annotation1791790/range1791790/1
type: Range
persistent identity: range1791790
display id: range1791790
version: 1
direction: direction/forward
top level: BBa_E70101
start: 1
end: 54
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1791802
display id: annotation1791802
version: 1
top level: BBa_E70101
location: https://synbiohub.org/public/igem/BBa_E70101/annotation1791802/range1791802/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_E70101/annotation1791802/range1791802/1
type: Range
persistent identity: range1791802
display id: range1791802
version: 1
direction: direction/forward
top level: BBa_E70101
start: 807
end: 886
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1791812
display id: annotation1791812
version: 1
top level: BBa_E70101
location: https://synbiohub.org/public/igem/BBa_E70101/annotation1791812/range1791812/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_E70101/annotation1791812/range1791812/1
type: Range
persistent identity: range1791812
display id: range1791812
version: 1
direction: direction/forward
top level: BBa_E70101
start: 895
end: 935
orientation: inline

BBa_K283025
type: ComponentDefinition
persistent identity: BBa K283025
display id: BBa_K283025
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: prosseA-rbs-RFP-terminator
creator: W Ling , Shi Lei, Nelson Chu
created: 2009-10-12T11:00:00Z
modified: 2015-05-08T01:11:47Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _383_
m user id: 0
owner id: 5494
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part is a generator for RFP under the prosseA promoter
mutable notes: BBa_K283025
mutable provenance: The prosseA promoter is self-designed The rbs, RFP and the terminator are from the registry
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_E1010
component: BBa_K283038
component: BBa_B0010
component: BBa_B0012
component: BBa_B0034
sequence annotation: BBa_B0034
sequence annotation: BBa_B0010
sequence annotation: BBa_K283038
sequence annotation: BBa_B0012
sequence annotation: BBa_E1010
sequence: https://synbiohub.org/public/igem/BBa_K283025_sequence/1

BBa_E1010
type: Component
persistent identity: component2256024
display id: component2256024
version: 1
top level: BBa_K283025
access: public
definition: https://synbiohub.org/public/igem/BBa_E1010/1

BBa_K283038
type: Component
persistent identity: component2256019
display id: component2256019
version: 1
top level: BBa_K283025
access: public
definition: https://synbiohub.org/public/igem/BBa_K283038/1

BBa_B0010
type: Component
persistent identity: component2256025
display id: component2256025
version: 1
top level: BBa_K283025
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
type: Component
persistent identity: component2256027
display id: component2256027
version: 1
top level: BBa_K283025
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0034
type: Component
persistent identity: component2256021
display id: component2256021
version: 1
top level: BBa_K283025
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2256021
display id: annotation2256021
version: 1
top level: BBa_K283025
location: https://synbiohub.org/public/igem/BBa_K283025/annotation2256021/range2256021/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K283025/annotation2256021/range2256021/1
type: Range
persistent identity: range2256021
display id: range2256021
version: 1
direction: direction/reverse
top level: BBa_K283025
start: 509
end: 520
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2256025
display id: annotation2256025
version: 1
top level: BBa_K283025
location: https://synbiohub.org/public/igem/BBa_K283025/annotation2256025/range2256025/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K283025/annotation2256025/range2256025/1
type: Range
persistent identity: range2256025
display id: range2256025
version: 1
direction: direction/forward
top level: BBa_K283025
start: 1241
end: 1320
orientation: inline

BBa_K283038
type: SequenceAnnotation
persistent identity: annotation2256019
display id: annotation2256019
version: 1
top level: BBa_K283025
location: https://synbiohub.org/public/igem/BBa_K283025/annotation2256019/range2256019/1
component: BBa_K283038

https://synbiohub.org/public/igem/BBa_K283025/annotation2256019/range2256019/1
type: Range
persistent identity: range2256019
display id: range2256019
version: 1
direction: direction/reverse
top level: BBa_K283025
start: 1
end: 500
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2256027
display id: annotation2256027
version: 1
top level: BBa_K283025
location: https://synbiohub.org/public/igem/BBa_K283025/annotation2256027/range2256027/1
component: BBa_B0012

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type: Range
persistent identity: range2256027
display id: range2256027
version: 1
direction: direction/forward
top level: BBa_K283025
start: 1329
end: 1369
orientation: inline

BBa_E1010
type: SequenceAnnotation
persistent identity: annotation2256024
display id: annotation2256024
version: 1
top level: BBa_K283025
location: https://synbiohub.org/public/igem/BBa_K283025/annotation2256024/range2256024/1
component: BBa_E1010

https://synbiohub.org/public/igem/BBa_K283025/annotation2256024/range2256024/1
type: Range
persistent identity: range2256024
display id: range2256024
version: 1
direction: direction/reverse
top level: BBa_K283025
start: 527
end: 1232
orientation: inline

BBa_K821001
type: ComponentDefinition
persistent identity: BBa K821001
display id: BBa_K821001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RFP Reporter with o-RBS
creator: Qinfeng Wu, Bin Jia
created: 2012-09-16T11:00:00Z
modified: 2015-05-08T01:13:29Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1079_
m user id: 0
owner id: 7151
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: RFP Reporter with o-RBS
mutable notes: Using the orthogonal system to regulate protein expression
mutable provenance: From BioBrick
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: RFP
sequence annotation: Terminator
sequence annotation: orthogonal RBS 1
sequence annotation: Lac Promoter
sequence: https://synbiohub.org/public/igem/BBa_K821001_sequence/1

RFP
type: SequenceAnnotation
persistent identity: annotation2184117
display id: annotation2184117
version: 1
top level: BBa_K821001
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K821001/annotation2184117/range2184117/1

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type: Range
persistent identity: range2184117
display id: range2184117
version: 1
direction: direction/forward
top level: BBa_K821001
start: 215
end: 895
orientation: inline

Terminator
type: SequenceAnnotation
persistent identity: annotation2184118
display id: annotation2184118
version: 1
top level: BBa_K821001
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K821001/annotation2184118/range2184118/1

https://synbiohub.org/public/igem/BBa_K821001/annotation2184118/range2184118/1
type: Range
persistent identity: range2184118
display id: range2184118
version: 1
direction: direction/forward
top level: BBa_K821001
start: 923
end: 1059
orientation: inline

orthogonal RBS 1
type: SequenceAnnotation
persistent identity: annotation2184116
display id: annotation2184116
version: 1
top level: BBa_K821001
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K821001/annotation2184116/range2184116/1

https://synbiohub.org/public/igem/BBa_K821001/annotation2184116/range2184116/1
type: Range
persistent identity: range2184116
display id: range2184116
version: 1
direction: direction/forward
top level: BBa_K821001
start: 203
end: 208
orientation: inline

Lac Promoter
type: SequenceAnnotation
persistent identity: annotation2184115
display id: annotation2184115
version: 1
top level: BBa_K821001
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K821001/annotation2184115/range2184115/1

https://synbiohub.org/public/igem/BBa_K821001/annotation2184115/range2184115/1
type: Range
persistent identity: range2184115
display id: range2184115
version: 1
direction: direction/forward
top level: BBa_K821001
start: 1
end: 194
orientation: inline

BBa_I2031
type: ComponentDefinition
persistent identity: BBa I2031
display id: BBa_I2031
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: GFP expression device controlled by an arabinose inducible pBAD promoter
creator: Bartholomew Canton
created: 2007-03-27T11:00:00Z
modified: 2015-08-31T04:07:40Z
discontinued: false
dominant: true
group u list: _11_
m user id: 0
owner id: 135
owning group id: 84
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part synthesizes GFP using a common RBS and terminator. The promoter is inducible by arabinose. This small molecule binds to the ara repressor (contained within I0500), drepressing the reporter.
mutable notes: n/a
mutable provenance: n/a
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_B0032
component: BBa_B0010
component: BBa_B0012
component: BBa_I0500
component: BBa_E0040
sequence annotation: BBa_E0040
sequence annotation: BBa_I0500
sequence annotation: BBa_B0012
sequence annotation: BBa_B0010
sequence annotation: BBa_B0032
sequence: https://synbiohub.org/public/igem/BBa_I2031_sequence/1

BBa_B0032
type: Component
persistent identity: component1922752
display id: component1922752
version: 1
top level: BBa_I2031
access: public
definition: https://synbiohub.org/public/igem/BBa_B0032/1

BBa_B0010
type: Component
persistent identity: component1922755
display id: component1922755
version: 1
top level: BBa_I2031
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
type: Component
persistent identity: component1922757
display id: component1922757
version: 1
top level: BBa_I2031
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_I0500
type: Component
persistent identity: component1922750
display id: component1922750
version: 1
top level: BBa_I2031
access: public
definition: https://synbiohub.org/public/igem/BBa_I0500/1

BBa_E0040
type: Component
persistent identity: component1922754
display id: component1922754
version: 1
top level: BBa_I2031
access: public
definition: https://synbiohub.org/public/igem/BBa_E0040/1

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation1922754
display id: annotation1922754
version: 1
top level: BBa_I2031
location: https://synbiohub.org/public/igem/BBa_I2031/annotation1922754/range1922754/1
component: BBa_E0040

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type: Range
persistent identity: range1922754
display id: range1922754
version: 1
direction: direction/reverse
top level: BBa_I2031
start: 1238
end: 1957
orientation: inline

BBa_I0500
type: SequenceAnnotation
persistent identity: annotation1922750
display id: annotation1922750
version: 1
top level: BBa_I2031
location: https://synbiohub.org/public/igem/BBa_I2031/annotation1922750/range1922750/1
component: BBa_I0500

https://synbiohub.org/public/igem/BBa_I2031/annotation1922750/range1922750/1
type: Range
persistent identity: range1922750
display id: range1922750
version: 1
direction: direction/reverse
top level: BBa_I2031
start: 1
end: 1210
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1922757
display id: annotation1922757
version: 1
top level: BBa_I2031
location: https://synbiohub.org/public/igem/BBa_I2031/annotation1922757/range1922757/1
component: BBa_B0012

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type: Range
persistent identity: range1922757
display id: range1922757
version: 1
direction: direction/forward
top level: BBa_I2031
start: 2054
end: 2094
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1922755
display id: annotation1922755
version: 1
top level: BBa_I2031
location: https://synbiohub.org/public/igem/BBa_I2031/annotation1922755/range1922755/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I2031/annotation1922755/range1922755/1
type: Range
persistent identity: range1922755
display id: range1922755
version: 1
direction: direction/forward
top level: BBa_I2031
start: 1966
end: 2045
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation1922752
display id: annotation1922752
version: 1
top level: BBa_I2031
location: https://synbiohub.org/public/igem/BBa_I2031/annotation1922752/range1922752/1
component: BBa_B0032

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type: Range
persistent identity: range1922752
display id: range1922752
version: 1
direction: direction/forward
top level: BBa_I2031
start: 1219
end: 1231
orientation: inline

BBa_I7102
type: ComponentDefinition
persistent identity: BBa I7102
display id: BBa_I7102
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Standard GFP expression device with sRBS
creator: Barry Canton
created: 2005-03-18T12:00:00Z
modified: 2015-08-31T04:07:45Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_6_
m user id: 0
owner id: 135
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_B0036
component: BBa_E0040
component: BBa_B0010
component: BBa_B0012
component: BBa_R0040
sequence annotation: BBa_E0040
sequence annotation: BBa_B0036
sequence annotation: BBa_B0012
sequence annotation: BBa_R0040
sequence annotation: BBa_B0010
sequence: https://synbiohub.org/public/igem/BBa_I7102_sequence/1

BBa_B0036
type: Component
persistent identity: component1438056
display id: component1438056
version: 1
top level: BBa_I7102
access: public
definition: https://synbiohub.org/public/igem/BBa_B0036/1

BBa_E0040
type: Component
persistent identity: component1438058
display id: component1438058
version: 1
top level: BBa_I7102
access: public
definition: https://synbiohub.org/public/igem/BBa_E0040/1

BBa_B0010
type: Component
persistent identity: component1438063
display id: component1438063
version: 1
top level: BBa_I7102
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
type: Component
persistent identity: component1438073
display id: component1438073
version: 1
top level: BBa_I7102
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_R0040
type: Component
persistent identity: component1438051
display id: component1438051
version: 1
top level: BBa_I7102
access: public
definition: https://synbiohub.org/public/igem/BBa_R0040/1

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation1438058
display id: annotation1438058
version: 1
top level: BBa_I7102
location: https://synbiohub.org/public/igem/BBa_I7102/annotation1438058/range1438058/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_I7102/annotation1438058/range1438058/1
type: Range
persistent identity: range1438058
display id: range1438058
version: 1
direction: direction/forward
top level: BBa_I7102
start: 74
end: 793
orientation: inline

BBa_B0036
type: SequenceAnnotation
persistent identity: annotation1438056
display id: annotation1438056
version: 1
top level: BBa_I7102
location: https://synbiohub.org/public/igem/BBa_I7102/annotation1438056/range1438056/1
component: BBa_B0036

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type: Range
persistent identity: range1438056
display id: range1438056
version: 1
direction: direction/forward
top level: BBa_I7102
start: 63
end: 67
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1438073
display id: annotation1438073
version: 1
top level: BBa_I7102
location: https://synbiohub.org/public/igem/BBa_I7102/annotation1438073/range1438073/1
component: BBa_B0012

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type: Range
persistent identity: range1438073
display id: range1438073
version: 1
direction: direction/forward
top level: BBa_I7102
start: 890
end: 930
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1438051
display id: annotation1438051
version: 1
top level: BBa_I7102
location: https://synbiohub.org/public/igem/BBa_I7102/annotation1438051/range1438051/1
component: BBa_R0040

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type: Range
persistent identity: range1438051
display id: range1438051
version: 1
direction: direction/forward
top level: BBa_I7102
start: 1
end: 54
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1438063
display id: annotation1438063
version: 1
top level: BBa_I7102
location: https://synbiohub.org/public/igem/BBa_I7102/annotation1438063/range1438063/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I7102/annotation1438063/range1438063/1
type: Range
persistent identity: range1438063
display id: range1438063
version: 1
direction: direction/forward
top level: BBa_I7102
start: 802
end: 881
orientation: inline

BBa_K1399022
type: ComponentDefinition
persistent identity: BBa K1399022
display id: BBa_K1399022
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ptet-GFP(DAS+2)
creator: Anna Stikane
created: 2014-09-18T11:00:00Z
modified: 2015-05-08T01:10:16Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1777_
m user id: 0
owner id: 22477
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: Tetracycline/aTc inducible promoter with GFP reporter tagged with NYADAS-ssrA degradation tag followed by terminator. The tagged GFP is actively degraded within cell, thus provides better temporal resolution of green fluorescence and promoter activity.
mutable notes: Part was assembled using BrickClip assembly (BBF RFC104) using other biobrick parts as templates. BrickClip assembly is a special case of more general Paperclip assembly method.[1]
mutable provenance: other parts
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_R0040
component: BBa_B0015
component: BBa_B0034
component: BBa_K1399007
sequence annotation: BBa_R0040
sequence annotation: BBa_B0015
sequence annotation: BBa_K1399007
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K1399022_sequence/1

BBa_R0040
type: Component
persistent identity: component2384279
display id: component2384279
version: 1
top level: BBa_K1399022
access: public
definition: https://synbiohub.org/public/igem/BBa_R0040/1

BBa_B0015
type: Component
persistent identity: component2384298
display id: component2384298
version: 1
top level: BBa_K1399022
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_B0034
type: Component
persistent identity: component2384285
display id: component2384285
version: 1
top level: BBa_K1399022
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K1399007
type: Component
persistent identity: component2384291
display id: component2384291
version: 1
top level: BBa_K1399022
access: public
definition: https://synbiohub.org/public/igem/BBa_K1399007/1

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2384279
display id: annotation2384279
version: 1
top level: BBa_K1399022
location: https://synbiohub.org/public/igem/BBa_K1399022/annotation2384279/range2384279/1
component: BBa_R0040

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type: Range
persistent identity: range2384279
display id: range2384279
version: 1
direction: direction/forward
top level: BBa_K1399022
start: 1
end: 54
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2384298
display id: annotation2384298
version: 1
top level: BBa_K1399022
location: https://synbiohub.org/public/igem/BBa_K1399022/annotation2384298/range2384298/1
component: BBa_B0015

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type: Range
persistent identity: range2384298
display id: range2384298
version: 1
direction: direction/reverse
top level: BBa_K1399022
start: 848
end: 976
orientation: inline

BBa_K1399007
type: SequenceAnnotation
persistent identity: annotation2384291
display id: annotation2384291
version: 1
top level: BBa_K1399022
location: https://synbiohub.org/public/igem/BBa_K1399022/annotation2384291/range2384291/1
component: BBa_K1399007

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type: Range
persistent identity: range2384291
display id: range2384291
version: 1
direction: direction/reverse
top level: BBa_K1399022
start: 81
end: 839
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2384285
display id: annotation2384285
version: 1
top level: BBa_K1399022
location: https://synbiohub.org/public/igem/BBa_K1399022/annotation2384285/range2384285/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1399022/annotation2384285/range2384285/1
type: Range
persistent identity: range2384285
display id: range2384285
version: 1
direction: direction/reverse
top level: BBa_K1399022
start: 63
end: 74
orientation: inline

BBa_J13025
type: ComponentDefinition
persistent identity: BBa J13025
display id: BBa_J13025
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: 3OC6HSL+LuxR dependent mRFP generator
creator: Jeff Tabor
created: 2005-07-17T11:00:00Z
modified: 2015-08-31T04:08:29Z
discontinued: false
dominant: true
experience: experience/None
group u list: _37_
m user id: 0
owner id: 88
owning group id: 37
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: dominantly repressed by lambda cI
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_R0065
component: BBa_B0034
component: BBa_E1010
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_E1010
sequence annotation: BBa_B0034
sequence annotation: BBa_B0010
sequence annotation: BBa_R0065
sequence: https://synbiohub.org/public/igem/BBa_J13025_sequence/1

BBa_R0065
type: Component
persistent identity: component1586358
display id: component1586358
version: 1
top level: BBa_J13025
access: public
definition: https://synbiohub.org/public/igem/BBa_R0065/1

BBa_B0034
type: Component
persistent identity: component1586370
display id: component1586370
version: 1
top level: BBa_J13025
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_E1010
type: Component
persistent identity: component1586377
display id: component1586377
version: 1
top level: BBa_J13025
access: public
definition: https://synbiohub.org/public/igem/BBa_E1010/1

BBa_B0012
type: Component
persistent identity: component1586393
display id: component1586393
version: 1
top level: BBa_J13025
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0010
type: Component
persistent identity: component1586383
display id: component1586383
version: 1
top level: BBa_J13025
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1586393
display id: annotation1586393
version: 1
top level: BBa_J13025
location: https://synbiohub.org/public/igem/BBa_J13025/annotation1586393/range1586393/1
component: BBa_B0012

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display id: range1586393
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direction: direction/forward
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start: 926
end: 966
orientation: inline

BBa_E1010
type: SequenceAnnotation
persistent identity: annotation1586377
display id: annotation1586377
version: 1
top level: BBa_J13025
location: https://synbiohub.org/public/igem/BBa_J13025/annotation1586377/range1586377/1
component: BBa_E1010

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type: Range
persistent identity: range1586377
display id: range1586377
version: 1
direction: direction/forward
top level: BBa_J13025
start: 124
end: 804
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1586370
display id: annotation1586370
version: 1
top level: BBa_J13025
location: https://synbiohub.org/public/igem/BBa_J13025/annotation1586370/range1586370/1
component: BBa_B0034

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type: Range
persistent identity: range1586370
display id: range1586370
version: 1
direction: direction/forward
top level: BBa_J13025
start: 106
end: 117
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1586383
display id: annotation1586383
version: 1
top level: BBa_J13025
location: https://synbiohub.org/public/igem/BBa_J13025/annotation1586383/range1586383/1
component: BBa_B0010

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type: Range
persistent identity: range1586383
display id: range1586383
version: 1
direction: direction/forward
top level: BBa_J13025
start: 838
end: 917
orientation: inline

BBa_R0065
type: SequenceAnnotation
persistent identity: annotation1586358
display id: annotation1586358
version: 1
top level: BBa_J13025
location: https://synbiohub.org/public/igem/BBa_J13025/annotation1586358/range1586358/1
component: BBa_R0065

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type: Range
persistent identity: range1586358
display id: range1586358
version: 1
direction: direction/forward
top level: BBa_J13025
start: 1
end: 97
orientation: inline

BBa_K1399013
type: ComponentDefinition
persistent identity: BBa K1399013
display id: BBa_K1399013
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Plac-RFP(DAS)
creator: Anna Stikane
created: 2014-09-18T11:00:00Z
modified: 2015-05-08T01:10:16Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1777_
m user id: 0
owner id: 22477
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: Lactose/IPTG inducible promoter with RFP reporter tagged with DAS-ssrA degradation tag followed by terminator. The tagged RFP is degraded within cell, thus provides better temporal resolution of red fluorescence and promoter activity. Presence of SspB is crucial for effective protein degradation.
mutable notes: Part was assembled using BrickClip assembly (BBF RFC104) using other biobrick parts as templates. BrickClip assembly is a special case of more general Paperclip assembly method.[1]
mutable provenance: other biobricks
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: start
sequence annotation: RFP
sequence annotation: BBa_B0015
sequence annotation: BBa_B0034
sequence annotation: stop
sequence annotation: LacI binding site
sequence annotation: polya
sequence annotation: BBa_R0010
sequence annotation: CAP binding site
sequence annotation: stem_loop
sequence annotation: -35
sequence annotation: conserved
sequence annotation: BBa_B0010
sequence annotation: BBa_K1399003
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: end of LacI coding region (inactive)
sequence annotation: -10
sequence annotation: barcode from BBa_E1010
sequence annotation: DAS-ssrA tag
sequence: https://synbiohub.org/public/igem/BBa_K1399013_sequence/1

start
type: SequenceAnnotation
persistent identity: annotation2384018
display id: annotation2384018
version: 1
top level: BBa_K1399013
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1399013/annotation2384018/range2384018/1

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type: Range
persistent identity: range2384018
display id: range2384018
version: 1
direction: direction/forward
top level: BBa_K1399013
start: 173
end: 173
orientation: inline

RFP
type: SequenceAnnotation
persistent identity: annotation2384022
display id: annotation2384022
version: 1
top level: BBa_K1399013
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1399013/annotation2384022/range2384022/1

https://synbiohub.org/public/igem/BBa_K1399013/annotation2384022/range2384022/1
type: Range
persistent identity: range2384022
display id: range2384022
version: 1
direction: direction/forward
top level: BBa_K1399013
start: 230
end: 901
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2384033
display id: annotation2384033
version: 1
top level: BBa_K1399013
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1399013/annotation2384033/range2384033/1

https://synbiohub.org/public/igem/BBa_K1399013/annotation2384033/range2384033/1
type: Range
persistent identity: range2384033
display id: range2384033
version: 1
direction: direction/reverse
top level: BBa_K1399013
start: 974
end: 1102
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2384020
display id: annotation2384020
version: 1
top level: BBa_K1399013
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1399013/annotation2384020/range2384020/1

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type: Range
persistent identity: range2384020
display id: range2384020
version: 1
direction: direction/reverse
top level: BBa_K1399013
start: 209
end: 220
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2384032
display id: annotation2384032
version: 1
top level: BBa_K1399013
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1399013/annotation2384032/range2384032/1

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type: Range
persistent identity: range2384032
display id: range2384032
version: 1
direction: direction/forward
top level: BBa_K1399013
start: 1095
end: 1095
orientation: inline

LacI binding site
type: SequenceAnnotation
persistent identity: annotation2384017
display id: annotation2384017
version: 1
top level: BBa_K1399013
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1399013/annotation2384017/range2384017/1

https://synbiohub.org/public/igem/BBa_K1399013/annotation2384017/range2384017/1
type: Range
persistent identity: range2384017
display id: range2384017
version: 1
direction: direction/forward
top level: BBa_K1399013
start: 166
end: 200
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation2384031
display id: annotation2384031
version: 1
top level: BBa_K1399013
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_K1399013/annotation2384031/range2384031/1

https://synbiohub.org/public/igem/BBa_K1399013/annotation2384031/range2384031/1
type: Range
persistent identity: range2384031
display id: range2384031
version: 1
direction: direction/forward
top level: BBa_K1399013
start: 1089
end: 1102
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation2384012
display id: annotation2384012
version: 1
top level: BBa_K1399013
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1399013/annotation2384012/range2384012/1

https://synbiohub.org/public/igem/BBa_K1399013/annotation2384012/range2384012/1
type: Range
persistent identity: range2384012
display id: range2384012
version: 1
direction: direction/forward
top level: BBa_K1399013
start: 1
end: 200
orientation: inline

CAP binding site
type: SequenceAnnotation
persistent identity: annotation2384014
display id: annotation2384014
version: 1
top level: BBa_K1399013
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1399013/annotation2384014/range2384014/1

https://synbiohub.org/public/igem/BBa_K1399013/annotation2384014/range2384014/1
type: Range
persistent identity: range2384014
display id: range2384014
version: 1
direction: direction/forward
top level: BBa_K1399013
start: 89
end: 126
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation2384028
display id: annotation2384028
version: 1
top level: BBa_K1399013
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1399013/annotation2384028/range2384028/1

https://synbiohub.org/public/igem/BBa_K1399013/annotation2384028/range2384028/1
type: Range
persistent identity: range2384028
display id: range2384028
version: 1
direction: direction/forward
top level: BBa_K1399013
start: 985
end: 1028
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2384024
display id: annotation2384024
version: 1
top level: BBa_K1399013
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1399013/annotation2384024/range2384024/1

https://synbiohub.org/public/igem/BBa_K1399013/annotation2384024/range2384024/1
type: Range
persistent identity: range2384024
display id: range2384024
version: 1
direction: direction/forward
top level: BBa_K1399013
start: 935
end: 937
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2384015
display id: annotation2384015
version: 1
top level: BBa_K1399013
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1399013/annotation2384015/range2384015/1

https://synbiohub.org/public/igem/BBa_K1399013/annotation2384015/range2384015/1
type: Range
persistent identity: range2384015
display id: range2384015
version: 1
direction: direction/forward
top level: BBa_K1399013
start: 137
end: 142
orientation: inline

conserved
type: SequenceAnnotation
persistent identity: annotation2384019
display id: annotation2384019
version: 1
top level: BBa_K1399013
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K1399013/annotation2384019/range2384019/1

https://synbiohub.org/public/igem/BBa_K1399013/annotation2384019/range2384019/1
type: Range
persistent identity: range2384019
display id: range2384019
version: 1
direction: direction/forward
top level: BBa_K1399013
start: 213
end: 216
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2384027
display id: annotation2384027
version: 1
top level: BBa_K1399013
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1399013/annotation2384027/range2384027/1

https://synbiohub.org/public/igem/BBa_K1399013/annotation2384027/range2384027/1
type: Range
persistent identity: range2384027
display id: range2384027
version: 1
direction: direction/forward
top level: BBa_K1399013
start: 974
end: 1053
orientation: inline

BBa_K1399003
type: SequenceAnnotation
persistent identity: annotation2384026
display id: annotation2384026
version: 1
top level: BBa_K1399013
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1399013/annotation2384026/range2384026/1

https://synbiohub.org/public/igem/BBa_K1399013/annotation2384026/range2384026/1
type: Range
persistent identity: range2384026
display id: range2384026
version: 1
direction: direction/reverse
top level: BBa_K1399013
start: 227
end: 940
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2384025
display id: annotation2384025
version: 1
top level: BBa_K1399013
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1399013/annotation2384025/range2384025/1

https://synbiohub.org/public/igem/BBa_K1399013/annotation2384025/range2384025/1
type: Range
persistent identity: range2384025
display id: range2384025
version: 1
direction: direction/forward
top level: BBa_K1399013
start: 938
end: 940
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2384029
display id: annotation2384029
version: 1
top level: BBa_K1399013
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1399013/annotation2384029/range2384029/1

https://synbiohub.org/public/igem/BBa_K1399013/annotation2384029/range2384029/1
type: Range
persistent identity: range2384029
display id: range2384029
version: 1
direction: direction/forward
top level: BBa_K1399013
start: 1062
end: 1102
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation2384030
display id: annotation2384030
version: 1
top level: BBa_K1399013
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1399013/annotation2384030/range2384030/1

https://synbiohub.org/public/igem/BBa_K1399013/annotation2384030/range2384030/1
type: Range
persistent identity: range2384030
display id: range2384030
version: 1
direction: direction/forward
top level: BBa_K1399013
start: 1069
end: 1088
orientation: inline

end of LacI coding region (inactive)
type: SequenceAnnotation
persistent identity: annotation2384013
display id: annotation2384013
version: 1
top level: BBa_K1399013
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1399013/annotation2384013/range2384013/1

https://synbiohub.org/public/igem/BBa_K1399013/annotation2384013/range2384013/1
type: Range
persistent identity: range2384013
display id: range2384013
version: 1
direction: direction/forward
top level: BBa_K1399013
start: 1
end: 88
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2384016
display id: annotation2384016
version: 1
top level: BBa_K1399013
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1399013/annotation2384016/range2384016/1

https://synbiohub.org/public/igem/BBa_K1399013/annotation2384016/range2384016/1
type: Range
persistent identity: range2384016
display id: range2384016
version: 1
direction: direction/forward
top level: BBa_K1399013
start: 161
end: 166
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2384021
display id: annotation2384021
version: 1
top level: BBa_K1399013
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1399013/annotation2384021/range2384021/1

https://synbiohub.org/public/igem/BBa_K1399013/annotation2384021/range2384021/1
type: Range
persistent identity: range2384021
display id: range2384021
version: 1
direction: direction/forward
top level: BBa_K1399013
start: 227
end: 229
orientation: inline

barcode from BBa_E1010
type: SequenceAnnotation
persistent identity: annotation2386313
display id: annotation2386313
version: 1
top level: BBa_K1399013
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1399013/annotation2386313/range2386313/1

https://synbiohub.org/public/igem/BBa_K1399013/annotation2386313/range2386313/1
type: Range
persistent identity: range2386313
display id: range2386313
version: 1
direction: direction/forward
top level: BBa_K1399013
start: 941
end: 965
orientation: inline

DAS-ssrA tag
type: SequenceAnnotation
persistent identity: annotation2384023
display id: annotation2384023
version: 1
top level: BBa_K1399013
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1399013/annotation2384023/range2384023/1

https://synbiohub.org/public/igem/BBa_K1399013/annotation2384023/range2384023/1
type: Range
persistent identity: range2384023
display id: range2384023
version: 1
direction: direction/forward
top level: BBa_K1399013
start: 902
end: 934
orientation: inline

BBa_I13574
type: ComponentDefinition
persistent identity: BBa I13574
display id: BBa_I13574
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pR + RFP + Terminator
creator: Jason Kelly
created: 2007-06-07T11:00:00Z
modified: 2015-08-31T04:07:35Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_84_
m user id: 0
owner id: 2
owning group id: 84
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: pR + RFP with 5' hairpin.
mutable notes: .
mutable provenance: .
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_B0034
component: BBa_R0050
component: BBa_B0015
component: BBa_E1010
sequence annotation: BBa_R0050
sequence annotation: BBa_E1010
sequence annotation: BBa_B0034
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_I13574_sequence/1

BBa_B0034
type: Component
persistent identity: component2252719
display id: component2252719
version: 1
top level: BBa_I13574
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_R0050
type: Component
persistent identity: component2252712
display id: component2252712
version: 1
top level: BBa_I13574
access: public
definition: https://synbiohub.org/public/igem/BBa_R0050/1

BBa_B0015
type: Component
persistent identity: component2252729
display id: component2252729
version: 1
top level: BBa_I13574
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_E1010
type: Component
persistent identity: component2252722
display id: component2252722
version: 1
top level: BBa_I13574
access: public
definition: https://synbiohub.org/public/igem/BBa_E1010/1

BBa_R0050
type: SequenceAnnotation
persistent identity: annotation2252712
display id: annotation2252712
version: 1
top level: BBa_I13574
location: https://synbiohub.org/public/igem/BBa_I13574/annotation2252712/range2252712/1
component: BBa_R0050

https://synbiohub.org/public/igem/BBa_I13574/annotation2252712/range2252712/1
type: Range
persistent identity: range2252712
display id: range2252712
version: 1
direction: direction/forward
top level: BBa_I13574
start: 1
end: 55
orientation: inline

BBa_E1010
type: SequenceAnnotation
persistent identity: annotation2252722
display id: annotation2252722
version: 1
top level: BBa_I13574
location: https://synbiohub.org/public/igem/BBa_I13574/annotation2252722/range2252722/1
component: BBa_E1010

https://synbiohub.org/public/igem/BBa_I13574/annotation2252722/range2252722/1
type: Range
persistent identity: range2252722
display id: range2252722
version: 1
direction: direction/reverse
top level: BBa_I13574
start: 82
end: 787
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2252719
display id: annotation2252719
version: 1
top level: BBa_I13574
location: https://synbiohub.org/public/igem/BBa_I13574/annotation2252719/range2252719/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I13574/annotation2252719/range2252719/1
type: Range
persistent identity: range2252719
display id: range2252719
version: 1
direction: direction/reverse
top level: BBa_I13574
start: 64
end: 75
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2252729
display id: annotation2252729
version: 1
top level: BBa_I13574
location: https://synbiohub.org/public/igem/BBa_I13574/annotation2252729/range2252729/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_I13574/annotation2252729/range2252729/1
type: Range
persistent identity: range2252729
display id: range2252729
version: 1
direction: direction/reverse
top level: BBa_I13574
start: 796
end: 924
orientation: inline

BBa_J5527
type: ComponentDefinition
persistent identity: BBa J5527
display id: BBa_J5527
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Arabinose -> mRFP
creator: Charles Yoon
created: 2006-10-30T12:00:00Z
modified: 2015-08-31T03:22:59Z
discontinued: false
dominant: false
group u list: _82_
m user id: 0
owner id: 840
owning group id: 82
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Arabinose will induce the pBad promoter and produce mRFP
mutable notes: none
mutable provenance: iGEM 2006 Registry
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_I13507
component: BBa_I0500
sequence annotation: BBa_I13507
sequence annotation: BBa_I0500
sequence: https://synbiohub.org/public/igem/BBa_J5527_sequence/1

BBa_I13507
type: Component
persistent identity: component2252294
display id: component2252294
version: 1
top level: BBa_J5527
access: public
definition: https://synbiohub.org/public/igem/BBa_I13507/1

BBa_I0500
type: Component
persistent identity: component2252282
display id: component2252282
version: 1
top level: BBa_J5527
access: public
definition: https://synbiohub.org/public/igem/BBa_I0500/1

BBa_I13507
type: SequenceAnnotation
persistent identity: annotation2252294
display id: annotation2252294
version: 1
top level: BBa_J5527
location: https://synbiohub.org/public/igem/BBa_J5527/annotation2252294/range2252294/1
component: BBa_I13507

https://synbiohub.org/public/igem/BBa_J5527/annotation2252294/range2252294/1
type: Range
persistent identity: range2252294
display id: range2252294
version: 1
direction: direction/reverse
top level: BBa_J5527
start: 1219
end: 2079
orientation: inline

BBa_I0500
type: SequenceAnnotation
persistent identity: annotation2252282
display id: annotation2252282
version: 1
top level: BBa_J5527
location: https://synbiohub.org/public/igem/BBa_J5527/annotation2252282/range2252282/1
component: BBa_I0500

https://synbiohub.org/public/igem/BBa_J5527/annotation2252282/range2252282/1
type: Range
persistent identity: range2252282
display id: range2252282
version: 1
direction: direction/reverse
top level: BBa_J5527
start: 1
end: 1210
orientation: inline

BBa_K1399020
type: ComponentDefinition
persistent identity: BBa K1399020
display id: BBa_K1399020
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ptet-GFP(LVA)
creator: Anna Stikane
created: 2014-09-18T11:00:00Z
modified: 2015-05-08T01:10:16Z
discontinued: false
dominant: false
group u list: _1777_
m user id: 0
owner id: 22477
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Tetracycline/aTc inducible promoter with GFP reporter tagged with LVA-ssrA degradation tag followed by terminator. The tagged GFP is actively degraded within cell, thus provides better temporal resolution of green fluorescence and promoter activity.
mutable notes: Part was assembled using BrickClip assembly (BBF RFC104) using other biobrick parts as templates. BrickClip assembly is a special case of more general Paperclip assembly method.[1]
mutable provenance: other biobricks
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_B0034
component: BBa_B0015
component: BBa_K1399004
component: BBa_R0040
sequence annotation: BBa_K1399004
sequence annotation: BBa_B0034
sequence annotation: BBa_B0015
sequence annotation: BBa_R0040
sequence: https://synbiohub.org/public/igem/BBa_K1399020_sequence/1

BBa_B0034
type: Component
persistent identity: component2384245
display id: component2384245
version: 1
top level: BBa_K1399020
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0015
type: Component
persistent identity: component2384258
display id: component2384258
version: 1
top level: BBa_K1399020
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_K1399004
type: Component
persistent identity: component2384251
display id: component2384251
version: 1
top level: BBa_K1399020
access: public
definition: https://synbiohub.org/public/igem/BBa_K1399004/1

BBa_R0040
type: Component
persistent identity: component2384239
display id: component2384239
version: 1
top level: BBa_K1399020
access: public
definition: https://synbiohub.org/public/igem/BBa_R0040/1

BBa_K1399004
type: SequenceAnnotation
persistent identity: annotation2384251
display id: annotation2384251
version: 1
top level: BBa_K1399020
location: https://synbiohub.org/public/igem/BBa_K1399020/annotation2384251/range2384251/1
component: BBa_K1399004

https://synbiohub.org/public/igem/BBa_K1399020/annotation2384251/range2384251/1
type: Range
persistent identity: range2384251
display id: range2384251
version: 1
direction: direction/reverse
top level: BBa_K1399020
start: 81
end: 833
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2384245
display id: annotation2384245
version: 1
top level: BBa_K1399020
location: https://synbiohub.org/public/igem/BBa_K1399020/annotation2384245/range2384245/1
component: BBa_B0034

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type: Range
persistent identity: range2384245
display id: range2384245
version: 1
direction: direction/reverse
top level: BBa_K1399020
start: 63
end: 74
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2384258
display id: annotation2384258
version: 1
top level: BBa_K1399020
location: https://synbiohub.org/public/igem/BBa_K1399020/annotation2384258/range2384258/1
component: BBa_B0015

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type: Range
persistent identity: range2384258
display id: range2384258
version: 1
direction: direction/reverse
top level: BBa_K1399020
start: 842
end: 970
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2384239
display id: annotation2384239
version: 1
top level: BBa_K1399020
location: https://synbiohub.org/public/igem/BBa_K1399020/annotation2384239/range2384239/1
component: BBa_R0040

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type: Range
persistent identity: range2384239
display id: range2384239
version: 1
direction: direction/forward
top level: BBa_K1399020
start: 1
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orientation: inline

BBa_I741100
type: ComponentDefinition
persistent identity: BBa I741100
display id: BBa_I741100
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter (I741015) and GFP (E0240)
creator: Lucien Weiss
created: 2007-06-20T11:00:00Z
modified: 2015-08-31T04:08:02Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _143_
m user id: 0
owner id: 1763
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable description: looks at promoter
mutable provenance: several different places
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_B0010
component: BBa_I741015
component: BBa_B0030
component: BBa_B0012
component: BBa_E0040
sequence annotation: BBa_I741015
sequence annotation: BBa_B0012
sequence annotation: BBa_B0010
sequence annotation: BBa_E0040
sequence annotation: BBa_B0030
sequence: https://synbiohub.org/public/igem/BBa_I741100_sequence/1

BBa_B0010
type: Component
persistent identity: component1935148
display id: component1935148
version: 1
top level: BBa_I741100
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_I741015
type: Component
persistent identity: component1935142
display id: component1935142
version: 1
top level: BBa_I741100
access: public
definition: https://synbiohub.org/public/igem/BBa_I741015/1

BBa_B0030
type: Component
persistent identity: component1935144
display id: component1935144
version: 1
top level: BBa_I741100
access: public
definition: https://synbiohub.org/public/igem/BBa_B0030/1

BBa_B0012
type: Component
persistent identity: component1935150
display id: component1935150
version: 1
top level: BBa_I741100
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_E0040
type: Component
persistent identity: component1935147
display id: component1935147
version: 1
top level: BBa_I741100
access: public
definition: https://synbiohub.org/public/igem/BBa_E0040/1

BBa_I741015
type: SequenceAnnotation
persistent identity: annotation1935142
display id: annotation1935142
version: 1
top level: BBa_I741100
location: https://synbiohub.org/public/igem/BBa_I741100/annotation1935142/range1935142/1
component: BBa_I741015

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type: Range
persistent identity: range1935142
display id: range1935142
version: 1
direction: direction/reverse
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start: 1
end: 301
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1935150
display id: annotation1935150
version: 1
top level: BBa_I741100
location: https://synbiohub.org/public/igem/BBa_I741100/annotation1935150/range1935150/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I741100/annotation1935150/range1935150/1
type: Range
persistent identity: range1935150
display id: range1935150
version: 1
direction: direction/forward
top level: BBa_I741100
start: 1147
end: 1187
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1935148
display id: annotation1935148
version: 1
top level: BBa_I741100
location: https://synbiohub.org/public/igem/BBa_I741100/annotation1935148/range1935148/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I741100/annotation1935148/range1935148/1
type: Range
persistent identity: range1935148
display id: range1935148
version: 1
direction: direction/forward
top level: BBa_I741100
start: 1059
end: 1138
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation1935147
display id: annotation1935147
version: 1
top level: BBa_I741100
location: https://synbiohub.org/public/igem/BBa_I741100/annotation1935147/range1935147/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_I741100/annotation1935147/range1935147/1
type: Range
persistent identity: range1935147
display id: range1935147
version: 1
direction: direction/reverse
top level: BBa_I741100
start: 331
end: 1050
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation1935144
display id: annotation1935144
version: 1
top level: BBa_I741100
location: https://synbiohub.org/public/igem/BBa_I741100/annotation1935144/range1935144/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_I741100/annotation1935144/range1935144/1
type: Range
persistent identity: range1935144
display id: range1935144
version: 1
direction: direction/forward
top level: BBa_I741100
start: 310
end: 324
orientation: inline

BBa_J07011
type: ComponentDefinition
persistent identity: BBa J07011
display id: BBa_J07011
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: ToxR promoter (ctx) controlling GFP
creator: bosworth
created: 2005-07-06T11:00:00Z
modified: 2015-08-31T04:08:19Z
discontinued: false
dominant: false
experience: experience/None
group u list: _21_
m user id: 0
owner id: 363
owning group id: 21
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part is a reporter construct designed to let us know the activity of the ToxR regulated promoter, ctx.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_J07007
component: BBa_B0030
component: BBa_B0010
component: BBa_B0012
component: BBa_E0040
sequence annotation: BBa_J07007
sequence annotation: BBa_B0012
sequence annotation: BBa_B0030
sequence annotation: BBa_B0010
sequence annotation: BBa_E0040
sequence: https://synbiohub.org/public/igem/BBa_J07011_sequence/1

BBa_J07007
type: Component
persistent identity: component1576795
display id: component1576795
version: 1
top level: BBa_J07011
access: public
definition: https://synbiohub.org/public/igem/BBa_J07007/1

BBa_B0030
type: Component
persistent identity: component1576803
display id: component1576803
version: 1
top level: BBa_J07011
access: public
definition: https://synbiohub.org/public/igem/BBa_B0030/1

BBa_B0010
type: Component
persistent identity: component1576811
display id: component1576811
version: 1
top level: BBa_J07011
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
type: Component
persistent identity: component1576821
display id: component1576821
version: 1
top level: BBa_J07011
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_E0040
type: Component
persistent identity: component1576806
display id: component1576806
version: 1
top level: BBa_J07011
access: public
definition: https://synbiohub.org/public/igem/BBa_E0040/1

BBa_J07007
type: SequenceAnnotation
persistent identity: annotation1576795
display id: annotation1576795
version: 1
top level: BBa_J07011
location: https://synbiohub.org/public/igem/BBa_J07011/annotation1576795/range1576795/1
component: BBa_J07007

https://synbiohub.org/public/igem/BBa_J07011/annotation1576795/range1576795/1
type: Range
persistent identity: range1576795
display id: range1576795
version: 1
direction: direction/forward
top level: BBa_J07011
start: 1
end: 145
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1576821
display id: annotation1576821
version: 1
top level: BBa_J07011
location: https://synbiohub.org/public/igem/BBa_J07011/annotation1576821/range1576821/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_J07011/annotation1576821/range1576821/1
type: Range
persistent identity: range1576821
display id: range1576821
version: 1
direction: direction/forward
top level: BBa_J07011
start: 991
end: 1031
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation1576803
display id: annotation1576803
version: 1
top level: BBa_J07011
location: https://synbiohub.org/public/igem/BBa_J07011/annotation1576803/range1576803/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_J07011/annotation1576803/range1576803/1
type: Range
persistent identity: range1576803
display id: range1576803
version: 1
direction: direction/forward
top level: BBa_J07011
start: 154
end: 168
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1576811
display id: annotation1576811
version: 1
top level: BBa_J07011
location: https://synbiohub.org/public/igem/BBa_J07011/annotation1576811/range1576811/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_J07011/annotation1576811/range1576811/1
type: Range
persistent identity: range1576811
display id: range1576811
version: 1
direction: direction/forward
top level: BBa_J07011
start: 903
end: 982
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation1576806
display id: annotation1576806
version: 1
top level: BBa_J07011
location: https://synbiohub.org/public/igem/BBa_J07011/annotation1576806/range1576806/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_J07011/annotation1576806/range1576806/1
type: Range
persistent identity: range1576806
display id: range1576806
version: 1
direction: direction/forward
top level: BBa_J07011
start: 175
end: 894
orientation: inline

BBa_J04451
type: ComponentDefinition
persistent identity: BBa J04451
display id: BBa_J04451
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RFP Coding Device with an LVA tag
creator: tammy674
created: 2005-06-08T11:00:00Z
modified: 2015-08-31T04:08:14Z
discontinued: false
dominant: false
experience: experience/None
group u list: _16_
m user id: 0
owner id: 328
owning group id: 16
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: This part contains an IPTG inducible promoter, RBS, RFP, terminator, and LVA tag.
mutable provenance: Davidson Synth-Aces
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_J04051
component: BBa_B0012
component: BBa_B0034
component: BBa_R0010
component: BBa_B0010
sequence annotation: BBa_B0034
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_R0010
sequence annotation: BBa_J04051
sequence: https://synbiohub.org/public/igem/BBa_J04451_sequence/1

BBa_J04051
type: Component
persistent identity: component1509844
display id: component1509844
version: 1
top level: BBa_J04451
access: public
definition: https://synbiohub.org/public/igem/BBa_J04051/1

BBa_B0012
type: Component
persistent identity: component1509859
display id: component1509859
version: 1
top level: BBa_J04451
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0034
type: Component
persistent identity: component1509830
display id: component1509830
version: 1
top level: BBa_J04451
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_R0010
type: Component
persistent identity: component1509820
display id: component1509820
version: 1
top level: BBa_J04451
access: public
definition: https://synbiohub.org/public/igem/BBa_R0010/1

BBa_B0010
type: Component
persistent identity: component1509849
display id: component1509849
version: 1
top level: BBa_J04451
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1509830
display id: annotation1509830
version: 1
top level: BBa_J04451
location: https://synbiohub.org/public/igem/BBa_J04451/annotation1509830/range1509830/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_J04451/annotation1509830/range1509830/1
type: Range
persistent identity: range1509830
display id: range1509830
version: 1
direction: direction/forward
top level: BBa_J04451
start: 209
end: 220
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1509849
display id: annotation1509849
version: 1
top level: BBa_J04451
location: https://synbiohub.org/public/igem/BBa_J04451/annotation1509849/range1509849/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_J04451/annotation1509849/range1509849/1
type: Range
persistent identity: range1509849
display id: range1509849
version: 1
direction: direction/forward
top level: BBa_J04451
start: 955
end: 1034
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1509859
display id: annotation1509859
version: 1
top level: BBa_J04451
location: https://synbiohub.org/public/igem/BBa_J04451/annotation1509859/range1509859/1
component: BBa_B0012

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type: Range
persistent identity: range1509859
display id: range1509859
version: 1
direction: direction/forward
top level: BBa_J04451
start: 1043
end: 1083
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation1509820
display id: annotation1509820
version: 1
top level: BBa_J04451
location: https://synbiohub.org/public/igem/BBa_J04451/annotation1509820/range1509820/1
component: BBa_R0010

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type: Range
persistent identity: range1509820
display id: range1509820
version: 1
direction: direction/forward
top level: BBa_J04451
start: 1
end: 200
orientation: inline

BBa_J04051
type: SequenceAnnotation
persistent identity: annotation1509844
display id: annotation1509844
version: 1
top level: BBa_J04451
location: https://synbiohub.org/public/igem/BBa_J04451/annotation1509844/range1509844/1
component: BBa_J04051

https://synbiohub.org/public/igem/BBa_J04451/annotation1509844/range1509844/1
type: Range
persistent identity: range1509844
display id: range1509844
version: 1
direction: direction/forward
top level: BBa_J04451
start: 227
end: 946
orientation: inline

BBa_K1399019
type: ComponentDefinition
persistent identity: BBa K1399019
display id: BBa_K1399019
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ptet-GFP(AAV)
creator: Anna Stikane
created: 2014-09-18T11:00:00Z
modified: 2015-05-08T01:10:16Z
discontinued: false
dominant: false
group u list: _1777_
m user id: 0
owner id: 22477
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: Tetracycline/aTc inducible promoter with GFP reporter tagged with AAV-ssrA degradation tag followed by terminator. The tagged GFP is actively degraded within cell, thus provides better temporal resolution of green fluorescence and promoter activity.
mutable notes: Part was assembled using BrickClip assembly (BBF RFC104) using other biobrick parts as templates. BrickClip assembly is a special case of more general Paperclip assembly method.[1]
mutable provenance: other biobricks
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_R0040
component: BBa_K1399005
component: BBa_B0015
component: BBa_B0034
sequence annotation: BBa_K1399005
sequence annotation: BBa_B0034
sequence annotation: BBa_R0040
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_K1399019_sequence/1

BBa_R0040
type: Component
persistent identity: component2384219
display id: component2384219
version: 1
top level: BBa_K1399019
access: public
definition: https://synbiohub.org/public/igem/BBa_R0040/1

BBa_K1399005
type: Component
persistent identity: component2384231
display id: component2384231
version: 1
top level: BBa_K1399019
access: public
definition: https://synbiohub.org/public/igem/BBa_K1399005/1

BBa_B0015
type: Component
persistent identity: component2384238
display id: component2384238
version: 1
top level: BBa_K1399019
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_B0034
type: Component
persistent identity: component2384225
display id: component2384225
version: 1
top level: BBa_K1399019
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K1399005
type: SequenceAnnotation
persistent identity: annotation2384231
display id: annotation2384231
version: 1
top level: BBa_K1399019
location: https://synbiohub.org/public/igem/BBa_K1399019/annotation2384231/range2384231/1
component: BBa_K1399005

https://synbiohub.org/public/igem/BBa_K1399019/annotation2384231/range2384231/1
type: Range
persistent identity: range2384231
display id: range2384231
version: 1
direction: direction/reverse
top level: BBa_K1399019
start: 81
end: 833
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2384225
display id: annotation2384225
version: 1
top level: BBa_K1399019
location: https://synbiohub.org/public/igem/BBa_K1399019/annotation2384225/range2384225/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1399019/annotation2384225/range2384225/1
type: Range
persistent identity: range2384225
display id: range2384225
version: 1
direction: direction/reverse
top level: BBa_K1399019
start: 63
end: 74
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2384219
display id: annotation2384219
version: 1
top level: BBa_K1399019
location: https://synbiohub.org/public/igem/BBa_K1399019/annotation2384219/range2384219/1
component: BBa_R0040

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type: Range
persistent identity: range2384219
display id: range2384219
version: 1
direction: direction/forward
top level: BBa_K1399019
start: 1
end: 54
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2384238
display id: annotation2384238
version: 1
top level: BBa_K1399019
location: https://synbiohub.org/public/igem/BBa_K1399019/annotation2384238/range2384238/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K1399019/annotation2384238/range2384238/1
type: Range
persistent identity: range2384238
display id: range2384238
version: 1
direction: direction/reverse
top level: BBa_K1399019
start: 842
end: 970
orientation: inline